LOCUS       DAOPSS010000001        51331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_490_length_51331_cov_9.192254, whole genome shotgun sequence.
ACCESSION   DAOPSS010000001 DAOPSS010000000
VERSION     DAOPSS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 51331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51331
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_490_length_51331_cov_9.192254"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PKN31_00005"
     CDS             complement(<1..1317)
                     /locus_tag="PKN31_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA98339.1"
                     /translation="MRQRVIVLAAVLVLVLGGGTTLAQKPTLEIWGRASFAPAQAYWF
                     NSLAYEWGAANGVDVKITWIPVADMSPKLATAIASGTEPDMVIGGYPTAKFAEADLLL
                     PLDDLVDKLGRDDFFEIKLKQGTVDGKVYSIPSGFELSWMHVRKDILEKAGVLDLLPF
                     ENEEGVLIAAEKANQVEPGVYGIGLPLGGSGADCYWTFQIYYYGFGGGFLSDRSVEGA
                     VFGKEPNRSIAKKTFAFEKDIYDRGLTPPDSGEWVDISNNMAFLEGRVAMTSNPMSIW
                     YGIMTGKPDLVPKTMIYPDAFPIDQGDESNFIFSSTPYPDLAKDLLYTFFGDKEVYRQ
                     GWCEQSQLYNLPVFKSQMQVLSENWKQGMYPFMGMDPYEAAMRTKFHTTPMAYPLGEA
                     SSVMEDLCWGWVINDMVIKAVIKGEDFDKVIDEFQVKLEDMVREAYG"
     gene            complement(1605..1763)
                     /locus_tag="PKN31_00010"
     CDS             complement(1605..1763)
                     /locus_tag="PKN31_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulforedoxin"
                     /protein_id="HOA98340.1"
                     /translation="MTEKKETYRCNVCGNVVEAKGGEEKEEELTCCGEPMVKVEEEPK
                     RGGGCCRK"
     gene            complement(1939..3273)
                     /locus_tag="PKN31_00015"
     CDS             complement(1939..3273)
                     /locus_tag="PKN31_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOA98341.1"
                     /translation="MGEKKSFEEKELFQIQEKEVKPLAFRMRPRSLDEVVGQPHLTGR
                     EGILRKIVETGFLPSLIFWGPPGSGKTSVAHIVAKACKYQFISISAVTSGVKEIREAV
                     KEAENDLKFHHKKTVFFIDEIHRFHKGQQSLLLPHVEEGIVTLIGSTTENPSFEIIAP
                     LLSRSQVIVFKKIPVEDLVRLLKNTLEDERGLKDEGIRVEEDALFFMAQLADGDARQA
                     LNFLEVASRIAQQENNGVIGIKFLQDTFHKTSYLYDKSGEEHFNLLSAYHKSLRGSDP
                     DAAIYWLARMLEGGEDPHAILRRLVACASEDVGNADPHALLIAVAAQKAYDFLGEPEG
                     RLSLAQATIYIASAPKSNASYQALNQALADVKEYGTLPVPLHLRNAPTGMMREMGFGK
                     EYRYPHDFTGAFVEQEYLPSQLKGKTYFHPHDRGYEKTMKERLRFLWKNQKY"
     gene            complement(3492..4535)
                     /locus_tag="PKN31_00020"
     CDS             complement(3492..4535)
                     /locus_tag="PKN31_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOA98342.1"
                     /translation="MKKLVDLRRDTNTLPTEEMKEAAFRSPLGDAVYGEDPRQRELEE
                     LAAFILGKERAIFLPSGTMGNLVALLTHTQRGNEIILEENAHIFTSETGGLAQVAGLM
                     VKTLRSEDGAPEPRDIERAIRPENIHYPRTALIGLENTHYRYGGIVVPLTRFEKIREV
                     ADRYNLPVHLDGARLFNAAIYLGVEAKEIARYCDSVMISLSKGLGAPVGSLLAGSCDF
                     IKEAERYRKMLGGGMRQTGWLCACGIIALSEDNISRLREDHDNAQFLAEGLQSLPGIK
                     IDRHQVQTNFVLADLSERELEATVFLERVREKGVLATRAGDFAVRFVTSREVNREDIV
                     FALQVIKEVTQVI"
     gene            complement(4532..6430)
                     /gene="aroF"
                     /locus_tag="PKN31_00025"
     CDS             complement(4532..6430)
                     /gene="aroF"
                     /locus_tag="PKN31_00025"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOA98343.1"
                     /translation="MIVSFNRKLSDLEKSELKEKLGKENLNPYFLEEETGKTVLVTEN
                     NFSSSQLDEKAVERTIKMDTPFQLAHRSFRPQGTVIKVKGVEIGGDNLTVMAGPCAVE
                     SEEQLFETAEIVKKMGAHILRGGAFKPRTSPYSFQGLGEKGLEILARAGEKFNLPIVT
                     EATSPEIADLVAQYADIIQIGARNMQNFELLKKVGFLKKPVLLKRGLSSTIEDWLLAA
                     EYILSWGNFQVILCERGIRTIERLTRNTLDLTAIPVVKDLSHLPVMVDPSHGTGLREK
                     VIPMARAAVACGADGVMVEVHPRPEKALSDGPQSLYPEQFKKLMNDLEVISILVGKEL
                     KKNDIPERMEIKSKPEFLNLRVAFLGESGSFSSQVARRAFGERAELLPCSNFREIFEK
                     VEQGEATHGLIPIENTITGSIHNNYDLLLEFPNLYVVGERFIRVSQYLGLYPGTKESE
                     IQVLFAHPQGFAQCAQFLESLRAVRVMNVGSTEEAARQAKEFGMGGSCISSEEAIKKY
                     GLELNQEEIEDNPRNFTRFLIVSPYFEPSSQDDKVSCVFAVGNYPGALHEALRIFADR
                     KVNLLKLESRPRPGKPWEYLFYVDWEGNLKEDMYQDMIKELSVKTSFWRVLGSYKNSW
                     KKAKEEKR"
     gene            complement(6559..7215)
                     /gene="hisIE"
                     /locus_tag="PKN31_00030"
     CDS             complement(6559..7215)
                     /gene="hisIE"
                     /locus_tag="PKN31_00030"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOA98344.1"
                     /translation="MEKSWEDVLFEKEELIPAIVQDAESGKVLMMAYMNREALSRTLE
                     TGKTWFYSRGRKKLWNKGESSGHFQEVQGIYVDCDNDTLLIQVKQEGVACHTGFFSCF
                     HREIKKGEISEPQAHFPPYSLSSFLQELFEVIEERAAHLSPNSYTSRLLEEGREKILR
                     KVAEEATEVILATFEDEDHNREHLGWEIADLLYHLLVLLQYEKMTYYQIMEELKKRRK
                     "
     gene            complement(7215..7994)
                     /gene="hisF"
                     /locus_tag="PKN31_00035"
     CDS             complement(7215..7994)
                     /gene="hisF"
                     /locus_tag="PKN31_00035"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOA98345.1"
                     /translation="MLARRIIPCLDVKEGKVVKGVHFENIKDVGDPVELAQRYEEEGA
                     DELVFLDITASYEQRETMIQVVEKIAEVLTIPFTVGGGIGATGQVANLLRAGADKISI
                     NTQAVLKPELISELAEMFGSQCVVVAIDVKRTWDRINRRSYWEVFINGGRTPTGKDAL
                     SWAQQVEKLGAGEILLTSMDTDGTQTGYDISLTRKVAERVRLPVIASGGAGKLEHLLA
                     ILREGKADAALVASIFHYRYFSIGEAKAYLQKRGIPVRVED"
     gene            complement(7997..8746)
                     /gene="hisA"
                     /locus_tag="PKN31_00040"
     CDS             complement(7997..8746)
                     /gene="hisA"
                     /locus_tag="PKN31_00040"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOA98346.1"
                     /translation="MGEELEEVILPIPAIDILEGKVVRLEKGDYQKVEVFSTSPLLVA
                     RKWEEEGAPFLHVVDLEGAKSGYPVNFPVIKELIQTVGIPVEVGGGIREVSSFMGYLD
                     AGAERVVLSSIVVKNPSVFEEMIKIAREKIVVSIDTRDQFVSLEGWREEIPVKAADLA
                     KDLKGKGIEHFIFTDVQRDGTLQGVRVEVIRDFIAESGVSIIVAGGISHKEDIRKIKS
                     LGEGVEGIILGKALYTGQLNFKEVCSILREE"
     gene            complement(8722..9330)
                     /gene="hisH"
                     /locus_tag="PKN31_00045"
     CDS             complement(8722..9330)
                     /gene="hisH"
                     /locus_tag="PKN31_00045"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOA98347.1"
                     /translation="MIAIVDYEIGNLYSIFKALERLGEEVVVTNQAQEIREAEAVVLP
                     GVGSFEKAMENLEKKEIKEVIKENQEDGKPFLGINLGLQLLFEKSQEAPRKKGLSLLP
                     GEVVKLPPTNKIPHMGWNRVYFKKNSPLTRDIPQGRFFYFAHSFYVLPEEPQAVVGIC
                     NYNVVIPAIVNHDNVWGFQFHPEKSSVWGMKVLENWVRSWKK"
     gene            complement(9352..9948)
                     /gene="hisB"
                     /locus_tag="PKN31_00050"
     CDS             complement(9352..9948)
                     /gene="hisB"
                     /locus_tag="PKN31_00050"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOA98348.1"
                     /translation="MVRLKKREALVKRKTRETDVEIYLSLDGEKNIQMDMAVPFFSHL
                     LESMAFYAGWDLKIKATAQEFIDDYHHVVEDTGIVLGEALNKCLGDKKGIERFATVFI
                     PMDEALSMVAVDLSGRPYFLWEKPDLGERVGSFEIVLVKEFLQALVNNAKITLHAKIW
                     WGDNGHHCLEALFKALGKSLAQAAKVTSVYLPSTKGTL"
     gene            complement(9962..11011)
                     /locus_tag="PKN31_00055"
     CDS             complement(9962..11011)
                     /locus_tag="PKN31_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA98349.1"
                     /translation="MESKIIPLFIPLFVVYLVNSGGIVLSLEPLDQAQWLLEKGEADK
                     VLALLEPLLDSFSEEELSQAVEIIYQIYSEKGESDKAIQTLEKYIDRFPNTPSSYLYR
                     YWIAKIEEERENFDKALELFYQIIDSYPPDVEDPYGLKNLVREDIAYNLQYHSKNYLE
                     AIKVYQEILDSNSDGEEKARIMMEIASCYEKLGKIEEAISYYQKVVREAPDFSYGRWA
                     ELRIVYLQSEPTWVEKSKEALAQKLEEAFRSKDIKKLESLARKGDFWVGVMFSEFEVD
                     EFYKVENYLSEHLSKATDLRVGKLEKRNDGFNLKIDNWPDPDYNILYLLIEEGLYGWE
                     WKGIILSNTELESLQ"
     gene            11408..12079
                     /locus_tag="PKN31_00060"
     CDS             11408..12079
                     /locus_tag="PKN31_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98350.1"
                     /translation="METFDFWRELKKNWNLIIRYPQIILVTLIPSLLIATSNYLTWQG
                     SLTWVSVFFQEAGSAILLILGVFLSFIALSLVIAIAWDYRYRDVIDLRRAWRIVQKRL
                     PDIVMVSLIMGFIEGFFAIWFMFLGLVFAFLLLFTIPLTVVEGDNPFSAIRNSFHLVS
                     ENLGECFTFFIIALFFLLIGYLLFWLLGFLGIAGLILNTVVGALILAYASLLLSSFYF
                     SLTRF"
     gene            complement(12076..12861)
                     /locus_tag="PKN31_00065"
     CDS             complement(12076..12861)
                     /locus_tag="PKN31_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA98351.1"
                     /translation="MDNFIKFLGTAGARVVVSKQLRASGGVWIKYQDTNLHIDPGPGA
                     LVRALSSRPPLSPPSLDALLLSHRHLDHSNDLNIMVEAMTEGATKKRGAVFLPGEALE
                     GEPVLFKYLREKLVSLEILEEGKEYHWKDISFSTPLRHLHSAETYGFRFSFPQGDVSF
                     ITDTLFEEKLIEAYQDSKILFLNTVRFKKDNHSSILHLSIPDAQVIIENIKPRLAFLT
                     HFGLTVLREKPWKIAEEMSQKTGIKVIAASDGMKIDLEEALKD"
     gene            complement(12861..14003)
                     /locus_tag="PKN31_00070"
     CDS             complement(12861..14003)
                     /locus_tag="PKN31_00070"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOA98352.1"
                     /translation="MWWRIELKDAYRIKKVKIPLSELKAGELLPFTLLNPQDQVLLSR
                     GQIITSEFVEKLKEDGYSSVLAEILEEDEGLVAKEDISPEIKKKTLENMQEILEDLSR
                     GETPSIVPLEQNVSQMAKEVKAHEELVIPLIQLKKHDDLTFTHSLNVAVISLFIAKFL
                     GLKDEELIHLGLGALLHDLGKLLIPPEILNKPSKLSEKDYEIIQKHTIFAKNILDEKT
                     RLPERVKEIPLQHHERIDGSGYILRLTKEETSVLSRITAVADVYEALTSDRPYRKALP
                     IPEVIEYLMGSAGHKLDDEVVTTFIRHLSPYQVGDLIKLSTGEEAMVSKINPVLPFRP
                     VIRIKKQDEQGNVYFSEEIDLSYSLTLTIIGEIDMKSQIDLERTIL"
     gene            14417..14767
                     /locus_tag="PKN31_00075"
     CDS             14417..14767
                     /locus_tag="PKN31_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98353.1"
                     /translation="MKQSTFKISFCTDVERMPRSSPFFLIKRLFFSLDRTKIKEWISL
                     PPPGWFYLYKYFIRKSKQCSSLFNPPIKCLVEEPQNKKNEKQRLNREMRPGVLTTLKT
                     HFLMKGEVLCDFCG"
     gene            complement(14789..16057)
                     /gene="rho"
                     /locus_tag="PKN31_00080"
     CDS             complement(14789..16057)
                     /gene="rho"
                     /locus_tag="PKN31_00080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOA98354.1"
                     /translation="MQLSLADLKSKTNAELFEIAQSLGISGYSRKRKNDLVFEILRKS
                     AESKGYLFSEGILEITPEGYGFLRTSDDFSPSDSDVYVSPSQIKRFMLSVGDKIAGQV
                     RPPKEGERYYALLRIEAINDEDPEQAKRRPLFENLTPIFPDEQLVLETVPEEISTRII
                     DLFVPIGKGQRGLIVSPPKAGKTVLLEKIANGIMANYPEVVLIVLLIDERPEEVTQME
                     RSVKGQVIASTFDQKPENHLRVAELTLERAKRLVEEGKDVVILLDSITRLARANNLVV
                     PTTGKTLSGGVDSSALHWPKRFFGAARNIEEGGSLTIIATALVDTGSRMDEVIYEEFK
                     GTGNMELHLSRALAESRIFPAIDIHRSGTRREELLLKKEDLEKIWMLRKLLSTVDAQE
                     ATQMVIERMKNTRSNREFLKIIDQVIKASR"
     gene            complement(16142..16891)
                     /locus_tag="PKN31_00085"
     CDS             complement(16142..16891)
                     /locus_tag="PKN31_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOA98355.1"
                     /translation="MTDAIVLAGGGEGEIEKKFGVVNRSLLVIKDKFMIEYVIEALKS
                     VSSIKRIIVVGPVKEIKCRIGSLVEEVVPPGENPFESAWKGLEALQTREKVLITGSDL
                     PLLKGEMVEDFLLRCSKEEADFYYPIIRREIYEKKLNDSQRTFVRLRDGCFTGGDILF
                     LNPQMMEKKKEWLSRIVESRKNPVALARLIGAKVIFKYLTKQLTIKDVEERVDKMLGI
                     KALAVITPYPEMGFDVDKVGHVQVAERILKK"
     gene            complement(16888..17790)
                     /gene="ispE"
                     /locus_tag="PKN31_00090"
     CDS             complement(16888..17790)
                     /gene="ispE"
                     /locus_tag="PKN31_00090"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOA98356.1"
                     /translation="MSEKFILPSYGKINWFLKIGETRSDGYHEIESLMQKIAFGDEIE
                     VSLEERERDYIACNFPIPTGEDGVLSRLLVELRKIAPPLREVGFNIKIKKSIPPGSGL
                     GGGSSNVASILQLINSVFKLNLDWEEVKSIAVRIGSDIPFFARGAPFAWVRGRGEEVE
                     PLFFAPHRFLVLLFPPFSISTAWAYRCWEERKGRIKENKKAGLSLEEFLKAKNPELIE
                     EIIWNDFEEVVFQCFPDLNFYKRLLMELGCKKVFMSGSGSTLVGVVESKEEGERIVKE
                     ISSPDVKAILTSTIWEAKEEGANQ"
     gene            complement(17783..18604)
                     /gene="murI"
                     /locus_tag="PKN31_00095"
     CDS             complement(17783..18604)
                     /gene="murI"
                     /locus_tag="PKN31_00095"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOA98357.1"
                     /translation="MEFKYPLGIIDSGIGGLSVVNAVRALLPAEDIIYVADPLHFPYG
                     EKSREELVEIVKPIIGYLSQHRKAKLIITACGTLSSVCLPDLAQLISIPIMGIVQSAC
                     QEAARVTRQGKIGVLATRATVKAGAFGQTLKGVNPGLEVIEEAWPEFVAAVEKGEFNS
                     PEWRVQIKNRLISLKDRGVDTLIMGCTHFSLISWFFQELGGRELQVVDPAWACAQEVK
                     NKLYSEEKRSSSSGKLSILVRGDGFSFQESLDNLSFHLKEKVSLFPVDLGVNISV"
     gene            complement(18594..20225)
                     /locus_tag="PKN31_00100"
     CDS             complement(18594..20225)
                     /locus_tag="PKN31_00100"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOA98358.1"
                     /translation="MDKGKYIFVTGGVASSLGKGITAASLGRILKSRGFRVTMQKFDP
                     YINVDAGTMNPYQHGEIFVTYDGGETDLDLGHYERFIDEELSKLSNVTTGKIYSSVIE
                     KERRGDYLGATVQVIPHITNQIKEEIFRLREMSKADISIVEIGGTVGDIEGLPFLEAI
                     RQIKNDIGKSNCLYIHVTLVPYLEAAGELKSKPTQHSVKELRSIGIQPDIIVCRSSHP
                     ITPEVMAKISLFCDLDKEAVIPLMDVDSIYEVPLVLEERNVGDLIASRLEMKPTPSDL
                     EEWSQIVQRVREADKEVNIGLVGKYTESKDAYLSVCEALKHGGIELGLKVSVKPIEAG
                     KLEGDEASCFLSGVDGIIIPGGFGQRGIEGKIQAVRYAREKEIPFLGICLGMQVATIE
                     FARNILHFHSAHSTEFDPLTPYPVIDLSPSQKDTQKMGGTMRLGNYRCVLESSLSKSL
                     YQKEEVWERHRHRYELNSLFVDELERAGLRMAGYNPDYQVVEIIELKDHPFFIGVQFH
                     PEFKSRPNQAHPLFRGFVEAAHCYRQERKGDRLGI"
     gene            complement(20316..21980)
                     /gene="argS"
                     /locus_tag="PKN31_00105"
     CDS             complement(20316..21980)
                     /gene="argS"
                     /locus_tag="PKN31_00105"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOA98359.1"
                     /translation="MSRLITEMLKEEIKKVLAVDFPEVVDEKEITVEPTRDKSHGDFA
                     TNLAFILSRKVKKPSRELATSLQLSLSRNLQNFARVEVAGGGFINFFLEDEILLSFIQ
                     EVEGEGEDFGAVNLGKGKKTQVEFVSVNPTGPLHVGHGKCAAFGDSLSRILKKAGFQV
                     EKEYYVNDAGRQIDLLGASLEARFRQLLGEKVEVPEEGYQGEYLIPIAEELLREKGSG
                     VLDLPPTDRSEVFQKFAVQKILDNICRDLADFRVHFDVWFRESSLYQKKEAEEILSLL
                     KERGYVYNKDGALWLRTTDWGDDKDRVLVRENGLPTYFASDIAYHWNKWKRGFQLVID
                     IWGADHHGYIPRMHAAMCALGLPEDFLEIFIVQFVTLLRGGQPERMSTRQGEFVPLRQ
                     LIDEVGVDVARYYFLMRDPATHLEFDIEEAKKQSMDNPVYYVQYAHARISSLFKEAEK
                     RGFTSFPLTHTLSEFPTEEEREIVKKIAYFPETVKRSALLRQPYLICNYLQDLCGDFH
                     SFYNLYRIIDEDNIELTSFRLSLCRAVQIVLKNAFQLLGISAPDTM"
     gene            22337..23815
                     /gene="xylB"
                     /locus_tag="PKN31_00110"
     CDS             22337..23815
                     /gene="xylB"
                     /locus_tag="PKN31_00110"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOA98360.1"
                     /translation="MEQFLGIDLGTQGVKVIGWDEEGNFLGSTSREYPILTPQIGFAE
                     QNPEIWWEKTKEAIKELVNLIPQVNVKAIGFSGQMHGTVIIDDNLKPLYPAIIWADQR
                     SHQEVKIIREKLEDKLATICGSDVATGFMAPTLLWLKENQREIFNRIRWAILPKDYLK
                     IKMGLTPSTDVADASSTLLFNIRKRNWSEEVLEILGLNDNIFPPVYESTQVIGQMKEE
                     VKEELGLKGEVLLVAGAGDQHCAALGNGITEEGELLITIGTGGQVFTPLCEPLVDDKL
                     RIHTFCHAIPGFWHLLGATLCAGLALSWFKKSVLDSPLQAFDFKELDQEAQKVEAGSH
                     GLLFLPYLIGERTPYMDPRARGSFLNLHLTHGRAHFIRAIMEGVCLGLKNVTEVFKEL
                     GVEPQRIVFSGGGAKSALWRKIMASVLDAPLFTTQVREEAATGACILAMMGTEKSSEE
                     EIISKIIRYGNPVLPERKAAEIYAEIYPKFRDAYPALKKLFP"
     gene            23828..24562
                     /gene="nadE"
                     /locus_tag="PKN31_00115"
     CDS             23828..24562
                     /gene="nadE"
                     /locus_tag="PKN31_00115"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOA98361.1"
                     /translation="MRDWKEETQKIITWMRKKVEEAKAQGLVLGMSGGIDSSVVAVLA
                     QKALGEKSLGIMMPCYSIPQDLEDALKVAQMFSIPHRVIPLEKPYDAFLEVLGEKQEN
                     SRSLALANLKPRLRMCTLYYFANRLNYLVCGASNRSEITVGYFTKYGDGGVDILPIAH
                     LTKTEVIELARFLNLPSFIITKPPSAGLWEGQTDEEEMGITYEKIDEFILHKKVEEPY
                     LSQIKSKIRASEHKRKMPATPEEITI"
     gene            complement(24555..26285)
                     /gene="polX"
                     /locus_tag="PKN31_00120"
     CDS             complement(24555..26285)
                     /gene="polX"
                     /locus_tag="PKN31_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOA98362.1"
                     /translation="MRNREVAQLLENIATLLEMKGENRFKIVAYEEAARRIESWPEPI
                     EEVWKEGRLKEIPGVGESIASKIEEYLSTGKLSYLEELTKEIPPEVVHLTAIPGVGPK
                     IAKLLYDQLGIKSIEDLEKAIAEQKLRHLPRLGVKSEEKIRKGIEMLKRQSGRMLLGQ
                     ALPLVEEIIEVIQEKSGVTKISPAGSIRRMKETIGDIDILVASSTPESIMDIFTSLPQ
                     VREIIARGTTKSSIVTQEGIQVDLRVVEETSFGAALQYFTGSKAHNIKLREIALKKGF
                     KVNEYGIFRLDNDKRVGGEKEEEIYETLGMEWIPPEMREDQGEIELAQEKKLPVLIEI
                     GDLQGDLHAHSNWSDGVASIEEMAEAALSRGYKYLAICDHTQSLAVASGLSPEEIRER
                     RKEIERWNKKGKGLRLLDGVEVNIMSDGSLDFPDSELEKLEVVVAGLHSGLGQGEEKI
                     LRRLEGAMKNKYVQVISHPTGRILNRRDAYQVNREKLFYLARETRTSLEINSQPERLD
                     LSDVDARRARDLGVKLVISTDAHSPSSLDYIRYGVAQARRGWLRKEDVLNTFSLEKLL
                     EFLNCKKKAK"
     gene            26354..28417
                     /locus_tag="PKN31_00125"
     CDS             26354..28417
                     /locus_tag="PKN31_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOA98363.1"
                     /translation="MGLFLIVGTAIFFNVSLSSANETDLYALFRQGDYETIISLKPEE
                     GEELFLYLVSLLNLSREEEVEKEISSLSPEKDYFWLDHLFYLSSLHCIENGDLEKAVI
                     WKERLANSFPSSPLLPYITLRLAQSFLENDFFSSALFYSIQVLTYSEREEDRLSALKI
                     TFQSLVNLGYLEEATLLLCHIYYVFPTLSSRETRELILPVLPQIFPEKFFPSQRLSLA
                     NFLFSLGFTEKTKDFLDQIEEFLPPSQQVDIFTLRARIFVREENWEELEQLISKNLLK
                     RGETEEVLFYWGVLEQRKANYGRAAQIYERLLFLYPESGYAFNTYQNLAFSLRAMGEE
                     GRYLETMEKIISRFPEKSAPVWELFWFYYNRNQMYSAQEMLEKLKSFSEEKNKALFWS
                     FKIDSSLEDLEEIVEGGKIDYYYARAWQELSDRGVVLPFPFDEEVPLPQVDPANISYS
                     EHWTKYQFLKKIEMWENAEIEILFLLSKNSQKKGFYRELNELYTQKGDYQKSILYSLY
                     LQEGERIPLNLARQIYPEFFLPSIQKLTGDELDPYLILALIRAESFFDSAAISSAGAM
                     GLAQVMPSTASWVIEKGWADGGGEEDISILLLNAEKNLEIGVSYFSYLWNRFGGKLYP
                     AICSYNAGPGRVDQWLETLSTDPDLFAESIPFSETQNYLKKVMANYFAYSIIYQGKFS
                     PPPYL"
     gene            complement(28414..29505)
                     /locus_tag="PKN31_00130"
     CDS             complement(28414..29505)
                     /locus_tag="PKN31_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOA98364.1"
                     /translation="MANLWGKEITREEILKRVGDISQIGGVKLAELSEGVERGVRIAE
                     VNSGGGLTYTVLLDRGMDIAWTSFKGMNVSWRSATRNLAPAFFEPEGLGWLRGFHGGL
                     MNTCGLTYAGAAGRDKSNHPFLEEEDFGLHGRASYLPASNLWVDGAWQGDDYLMWVQG
                     KIKESIVFGDKIALYRKIWSRLGGTTIHIEDQVVNEGWNESPFMILYHINFGYPLLDD
                     GARLFLPVEKTVPRDEMAEEGKEEWHLFSSPQKGYFEKVYLHYPKTLEDGYGASLLVN
                     DRLKMGAYVKFSKDTIPYFTEWKMMGEGEYVLGMEPGNCFPLGRRREREEGRLVTLQP
                     GESKKISLEIGILEGEEKIEQFKKYLKMS"
     gene            complement(29530..30837)
                     /gene="ftsZ"
                     /locus_tag="PKN31_00135"
     CDS             complement(29530..30837)
                     /gene="ftsZ"
                     /locus_tag="PKN31_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOA98365.1"
                     /translation="MKKKSLERGFSEKIDFKRELIRDTTKGIIREEDSIPEKEEQKAS
                     GEAVKKNLEKTKAKIEKKAEDRLAVIKVVGIGGGGNNAVNHMADLGLDGVDLIAVNTD
                     VQSLRKSLAQEKLQIGLSLTRGLGTGGDPRLGEEAARQDKERLSQLVENADLVFVAAC
                     MGGGTGTGAAPVLANISKESGALTIGVVTKPFDFEGLKRKRQAEEGISRLQQEVDALI
                     IIPNERLFQITKKDTPLQEAFRIADHILYQAVRGITDLITSPQEINLDLADLRTVLSE
                     AGMVLIGIGHGRGREKAKQAAEEAIQSPLLEISIKGAKSIILNITGGQDMTLSEVTEI
                     VNTVTNAAGTETDILWGYRVDESLSNETMVTVIATRFELPEEKLDEIEETIDEIDSRI
                     IIEDELDVPAFLREAQRKKLSEEKRPGDRISHFFHSDGRNDSE"
     gene            complement(30794..31600)
                     /locus_tag="PKN31_00140"
     CDS             complement(30794..31600)
                     /locus_tag="PKN31_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOA98366.1"
                     /translation="MRIIAIANQKGGVAKTTTCINLGASLAYHARKVLIIDMDPQAHS
                     TLGLGFEPDEFDRTILNVLEPPRSPYRLELKEVIIPTRTPNLYLAPANIDLAGAEYRL
                     IDKLGREDFLNSGIQRANLSFDYILIDCPPSLGILTVNALKACREVIITIQPHYFAMR
                     GIEEFMETIGLMKENLKHDLEVYVLITIADVRTNLYREVVEEIKKYFGDHLFETVIHR
                     NISLAEATSHGVPVMEYEPSSSGAQCYLNLAKEVIKLEEEKPGKRLLRKN"
     gene            complement(31640..33961)
                     /locus_tag="PKN31_00145"
     CDS             complement(31640..33961)
                     /locus_tag="PKN31_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98367.1"
                     /translation="MVVTVREETRFSLSLLGVAAVALAVETLTMRIFSFIFEYHFVSL
                     IISLALLGYGAAGSLYPFLPRWFKEHIPYFLALSLLLFAAGLVFLPLDIYQFWVNRYN
                     WFYFATLLFLAFLPFFAHGSYQLYAFELYPSHFPRFYAANLVGSSLGIGISYLILLFL
                     GELKALLFLALLSLVSATRVKKVPLFLLMIIGITFFFSPLEIYLSPYAPARAIREVEE
                     NQLIEVYHSPAELLEVFYTPYSRLALGLSSNFPDLPPPSWTLVYDKHQSQLFPVYPDF
                     SLLTHTLYYLPVDVLSPREILIVEGRKGLEGYLASFLELEKLDWVISSPLFASFLKDY
                     PLFYSEPQVIFPRKFLAQGRKYDLIFLEVPVAQAAVFPGSFSFQEDFLFTKEGIQSVF
                     SSLSREGVAVFSLFLQNPPAVLPKLVNLVKESWEEGERVEKHLMIIKNLDCALLMIKK
                     SPWEEKELKKMKEKVKKECFDFIYYPDIKEEEAEVVFNTQKRYYRVVEGILQGEDIES
                     TFDLRPPRDNRPYFSNFFRLRQLREAWINLGKRWLPFGGSGFWLVVLILVLVIALAFF
                     LILFPQKGEKGNIPSAVKILKGGGMLTGVGFMMMEISLFIKLEMIVGLPFYTFSLLLL
                     VLLVFSGLGSWRMRRGLDFREVKKLAWLHPLVLFAYSGFLLLLEEKLLHLPIWGSLLV
                     TFLPLAGVAYLCGLPFPLLSNLCSTFHPRFFGQVFAWNGFLSVISSLVVHLFSVFWGL
                     EIPLFLVVFIYLTFWLLMIFYYRIFALNCDMIS"
     gene            34038..34472
                     /gene="dut"
                     /locus_tag="PKN31_00150"
     CDS             34038..34472
                     /gene="dut"
                     /locus_tag="PKN31_00150"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOA98368.1"
                     /translation="MMKEVVIRFKKLSENAFLPRFSFPDDACFDLFSPIDTIIVPQES
                     KIIDLEIASEFPRGYEVVLRPRSGMGINHGIIVHLGTIDCGYRGSWMVRIFNLGKENY
                     SIKRGDRIIQGALREIPQVKIIETNSLSPSERGKQGLGSTGR"
     gene            34507..36240
                     /gene="glgP"
                     /locus_tag="PKN31_00155"
     CDS             34507..36240
                     /gene="glgP"
                     /locus_tag="PKN31_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOA98369.1"
                     /translation="MHIFDKLKNEPIIAYLSMEIALSSDMPTYSGGLGVLAGDTVRSA
                     ADLSLPFVAVTLISRKGYLNQKISPEGWQIEQPVEWEIEKILTPLPVQVEVEIEKRKV
                     KVGAWVYPWKSARGTIVPVIFLDTNIPQNTPEDRSITDYLYGGDSRYRLKQEIVLGIG
                     GSRILKALDLEVRKYHINEGHAALVTVELLQSFKNCDVEAVKNLCVFTTHTPVEAGHD
                     KFPYDMVKDVLGEPIPLPLLKQLGGADCLNMTLLALNLSGYINGVAKKHREVSLNMFP
                     GYDIHSITNGVHSYTWTSPGFQTLFDHYIPGWAQEPELLTRADIISNQEIWTVHQKEK
                     ARLLQLIKEKNNIEMDEEALTLGFARRMTAYKRPHFIFTDVERLKKINRAGKLQIVFA
                     GKAHPHDFEGKKLIQQIIQYSRELSPEIKIAFLENYNLEVAKKIIAGVDIWVNTPMRP
                     LEASGTSGMKAAHNGVINFSVLDGWWIEGCLEGITGWSIGPESEEDGNPQEIFQKEIS
                     DFYGKLEYVIVPTYYHHREQWIKLMKNSISKVASYFNAHRMMESYITEAYFHQPFLPL
                     ANGSIVKAEKN"
     gene            complement(36377..36580)
                     /locus_tag="PKN31_00160"
     CDS             complement(36377..36580)
                     /locus_tag="PKN31_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOA98370.1"
                     /translation="MATGKVKWFSATKGYGFIESDEGGDVFVHYSAIEGSGFKTLEQG
                     QEVEFEVQQGDKGPQAVKVKRLL"
     gene            complement(37130..38614)
                     /gene="gcvPB"
                     /locus_tag="PKN31_00165"
     CDS             complement(37130..38614)
                     /gene="gcvPB"
                     /locus_tag="PKN31_00165"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOA98371.1"
                     /translation="MNNPSEVPLIFEMSSPGRRGYEYPDCDVPSEKVTDLIPSRFLRK
                     KAADLPELSESEVVRHFTQLSRLNYGVDVGFYPLGSCTMKYNPKINEDVAKFAGFQRI
                     HPYQGEETVQGALKLMYELEKYLCEIAGLYRATMQPAAGAHGELTGLMIIRAFHRDRG
                     ENHKRQEIIVPDSAHGTNPASAAMCGYRVVEVKSNPRGGVDVLNLRKITGPQTAGLML
                     TNPNTLGLFEEDIVEIAHIVHQAGGLLYYDGANMNANLGISRPGDMGFDIVHYNLHKT
                     FAAPHGGGGPGSGPIAVTEELEDFLPVPLVSMKDNSYYLDYDRPKSIGKVKSFYGNFL
                     VMVKAYAYIRSLGAQGLREVSEIAVLNANYLMRQLQKDYHLPFDRSCKHEFVLSAKKQ
                     KAKGVRALDIAKRLLDYGFHPPTIYFPLVVEEALMVEPTETESKETLDRFGEAMREIA
                     LEAMENPEILKEAPHHTPLRRLDEVKAVKELDVNWYKRNPSSAM"
     gene            complement(38607..39953)
                     /gene="gcvPA"
                     /locus_tag="PKN31_00170"
     CDS             complement(38607..39953)
                     /gene="gcvPA"
                     /locus_tag="PKN31_00170"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOA98372.1"
                     /translation="MRYIPTTEKDREAMLKSLGISSIEELFNDIPEEVRFRGELNLPP
                     PQSEIEVLNSLRALAQKNADLDRYPSFLGAGAYDHLIPSAVQHILDRAEFYTSYTPYQ
                     PEISQGTLQAMYEYQSLICNLTEMDVANASMYDGATALAEAAILACGATGRGKALIAR
                     TIHPEYRQVTQLYLESRGLEAQYVPYDRGQVDLHSLKNNLDEDVACVLIQQPNFWGLL
                     EEVEEIASLAHENGALFIVCVNPISLGILEAPGNYGADIAVGEGQSLGGPVAFGGPFL
                     GFFAARDKFIRQMPGRIAGATNDAQGRRGFVLTLQTREQHIRRERATSNICSNQALNA
                     LAATVFLSLIGKEGLQEVANLCLQKAHYAYRKIVSIKGFAPLFPGAFFSEFPVSTPLP
                     PEKINKALWERGIIGGLDLKAYYPELGPAMLFSVTEARTREDIDALTQVLEEVANE"
     gene            complement(39996..40385)
                     /gene="gcvH"
                     /locus_tag="PKN31_00175"
     CDS             complement(39996..40385)
                     /gene="gcvH"
                     /locus_tag="PKN31_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOA98373.1"
                     /translation="MYPEDLLYTKSHEWVKVEDDRAIVGVTSYAQDQLVEVVFVELPE
                     EGKEVEAGDAVATLESVKAVADVYAPVSGKIIEVNKQLENQPELVNQDPYGEGWIFVI
                     EIAGEEELHTLMKVSDYQSLIENELKP"
     gene            complement(40404..41501)
                     /gene="gcvT"
                     /locus_tag="PKN31_00180"
     CDS             complement(40404..41501)
                     /gene="gcvT"
                     /locus_tag="PKN31_00180"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOA98374.1"
                     /translation="MEELRKTPLNHIHKKMGAKMIPFAGWEMPLLYETISSEHQKVRT
                     QAGIFDLTHMGELKIYGKGCELELAKLTTNDPTKLSPGKIQYSLLCLPSGGIIDDILV
                     YRLEDGFLLVVNAANTAKDYEWIKSHLREPVKVENLTDSLALIAIQGPDSEKIMVNIE
                     PAVSELKYYNFLETKIQNFRAILSRTGYTGEDGFEIYIPWDEAPKTWELLGEAAKIAG
                     LSLAPVGLGARDTLRLEMKYPLYGNDIDETTTPLEANLEWVVKFNHSFIGRDALFEQK
                     ASGVKKRLVGFVALDRGIPRQGYPIFYQEKKVGVVTSGTLSPSLKKNIGMGYVPPALE
                     KPGTVIELLIREKKVRAEITAGPFVPPRIKK"
     gene            41992..42441
                     /locus_tag="PKN31_00185"
     CDS             41992..42441
                     /locus_tag="PKN31_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOA98375.1"
                     /translation="MEISHILVPTDFSPFSDLALEHAASLAEKFGAKITILHVLTLAE
                     VNALTSQPGNPWENIVLKIQNNMREEFEKNAGNYSIVEEPVLEVAVGEPAEEIAKFAE
                     ENGVNLIVMGTHGRTGLASIFLGSVTIGVIKKTCLPIMVVKCAESLK"
     gene            complement(42618..43472)
                     /locus_tag="PKN31_00190"
     CDS             complement(42618..43472)
                     /locus_tag="PKN31_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOA98376.1"
                     /translation="MGNLFDSQVEIAWCPGCGNFGILRALKGALTELGFSPEEVVLVS
                     GIGQAAKLPHYIAANVFNGLHGRSIPVALAVKTVNPRLLVIAESGDGCMYGEGGNHFL
                     HALRRNPDITVIVHNNQVYGLTKGQASPTSEEGFVSKAQPWGNLSLPLNPLSLAISQD
                     ASFVARGFSGDVDKLKEIIKEALTWRGFSLIDVLQPCVTFNRVNTFAWYKERVYYLDD
                     SYQPEDRIKALEKSLEWGDKIPLGVIYQHQRPTFQDKIYSQIEEEDLIYRGVNFDDLF
                     SRGLRLFT"
     gene            complement(43465..45153)
                     /locus_tag="PKN31_00195"
     CDS             complement(43465..45153)
                     /locus_tag="PKN31_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOA98377.1"
                     /translation="MDLNLLVGGEAGQGVQTVSGALSTILQRSGYFVFSIEDYQSRIR
                     GGHSFTQVRFSTNPLRAALKEVDILACLNEETYQLHRRRVKEGGWVLGAVGNEREAHF
                     QHFLPLNFEKIAEGVGNKIFANIVAVGAILGILGLDLESADSYLEEIFARKGEEIVEK
                     NKQALRRGRDEVIPYVSSPLIEETRRGNSSLLVLDGNQALGWGALLSGCQFYSAYPMT
                     PSTGILNFISSHAKEYGVVVEQAEDEIAAINMAIGASYAGARAMTATSGGGFCLMAEG
                     LGLAAMTETPLVVVDAQRPGPSTGLPTRTSQGDLEFVIHASHDEFPRFVFAPRDPQEA
                     INLVIRAFNLAEKYQVPVIILSDQYLADSKWTYENLEVKERPDAPQFSSFPLPYRRYL
                     ISPSGVSPRAIPGLGKALVVADSDEHDEFGHLTEDLNLRVEMHKKRMRKKETMRKEVR
                     LPYHREGKDLTLIGWGSTWGVIEEAGERLKAEGIDSGIIHFSEIYPLPLQIEELFSHV
                     SLPVVVENNYGGQLANLLERELKLPFPFRINSYNGRPFLVEELLLKVKEVGKNG"
     gene            complement(45509..46318)
                     /locus_tag="PKN31_00200"
     CDS             complement(45509..46318)
                     /locus_tag="PKN31_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOA98378.1"
                     /translation="MPETSVSILETVLIRALNIAIIVGVSYFILSISERLIRKILSWG
                     GKKYTDRRELARRVNTLVPLLRSILKYVVIFFAAILILRQLGVDATAIIAGAGIVGIA
                     VGFGAQTLVKDVLTGFFLLLEDSISVGDTVQIGDITGTVEDIGMRVTRIRPFSGALIV
                     VPNGEISRIANYNRGFSRAIVEVGIAYEADIDQAISVLEEVAEKYHREHPRRILEPAT
                     IHRIVRLDSSRVVLRLVLKVAPNEHWDVERDILYLIKKAFDQEGIKIVMLQ"
     gene            complement(46318..49587)
                     /gene="carB"
                     /locus_tag="PKN31_00205"
     CDS             complement(46318..49587)
                     /gene="carB"
                     /locus_tag="PKN31_00205"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOA98379.1"
                     /translation="MAKREDIQKILIIGSGPIVIGQACEFDYSGVQGCKALKSEGYKV
                     VLVNSNPATIMTDPEMADRTYIEPLVPEILEKIIEREQPDALLPTLGGQTALNLTVQL
                     EERGVLNKWGVEVLGASIQAIKKAEDRRLFKESMTKVGIEVPRSGQAYSLEEAIRIAK
                     EIGFPLVIRPSFTLGGTGGAIAYNIEEFEDLAQQALDSSLIREILIEESVAGWKEFEL
                     EVMRDAKDNVVVICSIENFDPMGIHTGDSITVAPAQTLTDQEYELMRDLAIKAIREIG
                     VDTGGCNIQFAVNPQNGRIVIIEINPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     IPNDITRKTPASFEPALDYCVVKIPRFTFEKFPETEPWLGISMKSVGETMAIGRNFKE
                     ALQKGLRSLEIGKYGLEDIKNWEDFSPGKRQEILRERLAKPNAERVFYIKMAIKEGLN
                     IEEIYNLTKIDPWFIHEIVEIVEMEEELKHIKDWKNLNPESGKRAKEFGFSDHQLAEF
                     WNTEEEEVRRWREEKGIEAVYKQVDTCAGEFPAETPYYYSTYEEECEANPSKREKAII
                     LGAGPNRIGQGIEFDYCCVHATWGLRDLGYETIMVNSNPETVSTDYDTSDKLFFEPVY
                     LEDVLNVVEKENPHGVILQLGGQTPLNLAKGLERAGVKILGTSPESIDMAEDRDRFKE
                     LVDKIGLKQPRSGIATSFEEAHLVAAQIGYPVMVRPSYVLGGRAMRIIYSEEELKEFI
                     SQAVEVSAGHPILIDKFLEDAIELDVDAISDGETTIIAGIMEHIEEAGIHSGDSACVI
                     PPFSLSDEIIEKVKQITYTLARELKVVGLMNVQYAARDDEVFVLEVNPRASRTIPFVS
                     KATGVPYARLASQVMAGRKLREIGLTEEVKIDYFAVKEAVFPFRRFSGVDPVLGPEMR
                     STGEVMGIDSDFGMAFAKSQIAAQFYLPSEGTVFISVKNRDKRGIIFIAKKLRDLGFK
                     IVATRGTAKVLSNNGIPVEIVEKVGEGRPNVVDVIKNRQIDLIINTPSGGRAQLESSI
                     IRKEAIIRSIPFVTTLSGAEATVYAIERLKSGSITVKSIQEYHQEIFERQGITLALRR
                     D"
     gene            complement(49580..50659)
                     /gene="carA"
                     /locus_tag="PKN31_00210"
     CDS             complement(49580..50659)
                     /gene="carA"
                     /locus_tag="PKN31_00210"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOA98380.1"
                     /translation="MKALLALEDGRIFQGYAFGTEGEAGGEVIFDTGMTGYQEVLTDP
                     SFRGQIVTITYPLVGNCGINSQDSESSRPQVEALIVREKSTIYSSWRGEETLEEYFRR
                     HNLLGLERIDTRALTRHIREKGALRGVISTRDSDPASLIKKARQIPPMIGRDLVREVT
                     TPAPYVWQEEGKFNILVVDCGVKFSILKELAKRNCRVVVYPATSPVEEIIKEKPHGVV
                     ISNGPGDPASLSYLVDLVQKIMERFPIMGICIGHQIMAQAVGGKTSRLKFGHHGTNHP
                     VKDLRSGWVAITTQNHGFVVDSDTLPPEVEVTHINLNDFTLEGMRHKRLPFFSVQFHP
                     EAHPGPHDTVYLFDEFIKMVEDNLG"
     gene            51142..51218
                     /locus_tag="PKN31_00215"
     tRNA            51142..51218
                     /locus_tag="PKN31_00215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51176..51178,aa:Arg,seq:tcg)
BASE COUNT        14991 a        11961 c         9169 g        15210 t
ORIGIN      
        1 tccgtatgct tcccgcacca tatcttctag tttgacctga aattcatcga ttactttgtc
       61 aaagtcttcc ccctttatca ctgcctttat taccatgtca ttaataaccc atccccagca
      121 caggtcttcc attactgacg aagcttcccc taaaggataa gccattgggg tagtgtgaaa
      181 cttggttcgc atagctgctt cataagggtc cattcccata aacggataca taccttgttt
      241 ccaattttca ctcaacactt gcatctggct tttaaatacc ggaaggttat aaagttggga
      301 ttgctcacac cacccctggc gataaacttc tttgtcacca aagaaagtgt aaagaaggtc
      361 tttagctaaa tcaggataag gggttgaact aaaaataaag ttactttcat cgccttggtc
      421 gatgggaaaa gcatcaggat agatcatggt tttaggtact aaatctggtt ttcccgtcat
      481 tatcccatac cagatactca taggattaga ggtcatagct actcttccct ccaggaaagc
      541 catattatta gatatatcta cccattcccc tgaatcagga ggggttaaac cccgatcata
      601 aatatctttt tcgaaagcga atgttttttt agctatggaa cgattaggct ctttcccaaa
      661 cactgctcct tctactgacc tatcactcaa gaatcctcct ccaaaaccat agtaataaat
      721 ctggaaagtc caatagcaat cagctccact tcctcccaaa gggagaccta ttccataaac
      781 tcccggctcc acttgattgg ctttctcagc tgctataagc actccctctt cgttttcaaa
      841 aggcaacaag tcgagaacac cggctttttc caggatatct ttgcggacat gcatccagct
      901 tagctcaaat cctgagggga tagaataaac ttttccgtct accgtacctt gtttaagctt
      961 aatctcaaag aaatcatctc tgcccagttt atctaccaga tcatcgaggg gtaacaaaag
     1021 atcagcttcg gcaaacttgg ctgtggggta acctccaata accatatctg gttcggttcc
     1081 tgacgctata gctgtggcta atttcgggga catatctgct actggaatcc aggtgatttt
     1141 tacgtccact ccgttggctg ctccccattc gtaagctaaa gagttaaacc agtaggcttg
     1201 agccggagca aaagaagcgc gtccccaaat ttctaaggtg ggtttttgag ctaaagttgt
     1261 cccccctccc aacaccaaca ccaacaccgc agccaaaaca ataactctct gtctcatggc
     1321 tgaatccctc cttttttagt tagtgaaacc agagaaatta aatagctttt acctttttct
     1381 cacctccctc ctctttttct ggtttctgca aaaacgaaca gctacagtgt aaaattaaaa
     1441 gtaaaagatg aaaaagtaac atcataataa taccacagtg ttcatcctct ggtattatta
     1501 tgatgttaga aatggattaa acagaaagat agagaaatca caacaatttt tgcgactaag
     1561 cgtatagaat tatgcgggct acctgctctt aattattagt tactttactt tctgcagcaa
     1621 ccgccacctc ttttaggctc ctcctcaact ttaaccatag gctcaccaca gcaagttagt
     1681 tcttcttctt tttcttctcc tcctttagct tccactacgt taccacacac attgcaccga
     1741 taagtttctt ttttttctgt cataagattc cacctcctga gatattttac ccttaaaaca
     1801 ttcattattc aacttatcgt ggcaaccaaa aatgaaaaaa aataatgacc agcatctctt
     1861 tccattttaa gcatgtcttt tatagttttc ttacttacta tgaaattaga gaagaaggaa
     1921 aaaccacgcg aagctaattc agtacttctg gtttttccac aaaaaacgca acctttcttt
     1981 cattgtcttt tcgtatcccc tatcatgggg atgaaagtag gttttcccct ttagttgaga
     2041 aggtaaatat tcctgttcta caaatgctcc agtaaagtcg tgaggatatc gatattcctt
     2101 accaaatccc atctccctca tcataccagt aggggcatta cgcaggtgca agggaacagg
     2161 aagcgtacca tactccttca catccgccaa cgcctggttc aacgcctgat aagaagcatt
     2221 gcttttagga gcgctggcta tatatatagt ggcctgagct aaagacagcc ttccttccgg
     2281 ctctcccaga aaatcatagg ctttttgagc agcgacagct atcagcaaag catgaggatc
     2341 agcattaccc acatcttctg aagcacaggc taccagacga cgtaaaatag catgagggtc
     2401 ttctcctccc tccagcatac gagctaacca gtaaatagca gcatcagggt cactacctcg
     2461 caagctctta tgataagcag aaagaagatt aaaatgctct tctcctgatt tatcgtataa
     2521 ataggaggtt ttatgaaaag tatcctgtaa aaacttaatc cctattaccc cgttgttttc
     2581 ttgttgagca atacgagaag caacctccag aaagttcaaa gcctgccgag catccccatc
     2641 agcaagctga gccatgaaga aaagagcgtc ttcctccacc cttattccct catcttttaa
     2701 tcctcgttcg tcttctaagg tattcttcaa caagcgaacc agatcttcta ccggaatctt
     2761 tttaaaaact attacctgag aacgagaaag caagggagca ataatttcaa aagaggggtt
     2821 ttcggtagta gaaccaatca aagtaactat tccttcttcc acgtggggaa gaagtaagct
     2881 ttgttgtcct ttatgaaagc gatgaatctc atcaataaaa aaaactgttt tcttatggtg
     2941 aaacttcagg tcattttctg cttcttttac tgcctctctt atttccttta ctcctgaggt
     3001 aactgcactg atggaaataa actgatattt acaagcctta gccacaatat gagcaacaga
     3061 agtcttacca ctccccggtg gcccccaaaa aatgagagaa ggtaaaaaac cagtttccac
     3121 tattttcctc agaatacctt cccttccagt aaggtgaggt tgacctacta cctcatccag
     3181 actacgagga cgcatccgaa aggcaagcgg tttcacttct ttttcttgaa tctgaaataa
     3241 ttccttttcc tcaaaagatt ttttctctcc cacgctaagg cacccagttt ccccctcgtc
     3301 aaactattgt tctaaaacaa acaaattata ctatttaatt aaaaattaag caacttcttc
     3361 actttcttgc tcttctctat ctatggtttc tgtttcaatt cgagaaataa aagaggatgc
     3421 ttttctttgt tctttttgaa cattatttag gattgttttt atgcttttct ctactgagat
     3481 ttaactaaac atcaaatgac ttgtgtaact tctttgataa cctgaagagc aaagacaata
     3541 tcttctctgt ttacttcccg ggaagttacg aaacgaactg cgaaatcccc ggctcgagtg
     3601 gccagaactc ctttctctct gactctttcc agaaaaacag tagcctctaa ttctctctca
     3661 cttaaatcgg ctaaaacaaa gttggtttgt acttgatgcc gatctatttt tatccctggt
     3721 aaagactgca gaccttccgc caaaaattgg gcgttatcat ggtcttcccg gaggcgagaa
     3781 atgttgtcct cagaaagagc aatgatacca caggcacaca accaaccagt ctgtctcatt
     3841 cctcctccca gcattttgcg gtatctctct gcctctttta taaaatcgca agacccggcg
     3901 agtagcgaac ccaccggagc tcctaagcct ttggaaagag aaatcataac tgaatcacaa
     3961 tagcgagcaa tctctttagc ttctactccc agataaattg cagcattaaa aagccttgcc
     4021 ccatcaagat gaacaggcaa attgtatctg tctgctacct ctcttatttt ctcaaatctg
     4081 gtaagaggaa ccactatgcc tccatagcga taatgagtat tttctaagcc aattagagcc
     4141 gtcctagggt aatggatatt ctccggtcgg atagctctct caatatcccg aggttctggc
     4201 gctccatctt cacttcgtaa agttttaacc attaaacccg ctacttgggc taaccctcca
     4261 gtttctgaag tgaaaatatg ggcattctct tccaggatta tctcgttgcc tctctgagta
     4321 tgagtaagga gagcaaccag attacccatg gtcccactgg ggaggaaaat tgctctttct
     4381 tttcccagaa taaaggcagc taattcctcc agctctcttt gccttggatc ttcaccgtaa
     4441 accgcatccc ccaaaggaga gcggaaggct gcctctttca tctcctcagt tggaagagta
     4501 ttggtatccc ggcgcaaatc taccagcttt ttcatctctt ttcctcttta gctttcttcc
     4561 aggaattttt ataacttccc agaacacgcc agaagctggt tttaacagat aattctttta
     4621 tcatatcctg atacatgtct tcctttaaat ttccttccca atctacataa aagagatatt
     4681 cccagggttt tcccggtcga gggcgagatt cgagtttcaa caggttaact ttccgatcgg
     4741 caaaaatacg aagcgcttca tgaagtgccc cgggataatt ccctacagca aaaacacaag
     4801 aaactttatc atcttgagaa gaaggttcaa aataaggaga aacaatcaaa aaacgagtaa
     4861 agttacgagg attatcttca atctcttcct gatttaattc cagaccatat ttttttatgg
     4921 cctcttcaga agaaatacaa gatcccccca ttccaaattc tttggcctga cgagctgctt
     4981 cttcagtgct ccccacattc attactcgca ccgccctcaa cgattcgaga aattgagcac
     5041 actgggcaaa accctgagga tgagcaaata gcacctgaat ctcactctct ttagtaccag
     5101 gataaaggcc caggtactga gaaacgcgaa tgaatcgctc tcccactaca taaagattgg
     5161 gaaattctaa aaggagatcg taattattat gaatactacc ggtgatagta ttttctatgg
     5221 gtatcaaccc gtgagtagct tctccctgct ccactttttc gaatatttct cgaaaattag
     5281 agcaaggtaa taattcggcc ctctctccaa aagcacgacg agccacttga gagctaaaag
     5341 aaccgctttc tcccaaaaaa gccactctca aattcaaaaa ctcgggtttg ctttttatct
     5401 ccattctctc tggtatatcg ttctttttta gttccttacc caccagaata cttatcactt
     5461 ccaggtcgtt catcaatttt ttaaattgct cagggtagag gctttgcgga ccatcagaga
     5521 gagctttttc tggacgggga tggacttcta ccattactcc atctgctcca caagctacag
     5581 ctgctcgagc catgggaatc actttttccc gcagacccgt cccatgacta gggtctacca
     5641 ttaccggtaa atgagaaaga tctttaacta ccgggatagc ggtcaaatcc agagtgttac
     5701 gagtaagcct ttcaatagtt ctaatacccc gctcacagag aatcacctgg aaatttcccc
     5761 agctcaagat atattcagca gccagtaacc aatcttctat ggtagaagat aaacctcgct
     5821 tcaggagcac cggctttttc aaaaagccca ctttcttgag aagctcaaag ttttgcatat
     5881 tgcgagctcc aatctgaatg atatcagcat attgtgccac aaggtcagca atttcaggac
     5941 tggtagcttc ggtaactatg ggtaaattaa atttttctcc tgctcgagcc agaatctcaa
     6001 gccctttctc tcccagacct tgaaagctat aaggggaagt gcgaggttta aaagctcctc
     6061 ctcgaagaat atgggctccc atttttttca ctatttctgc agtttcaaaa agttgttctt
     6121 ccgattctac agcacaggga ccagccataa ccgtgagatt atcccctcct atttccactc
     6181 cttttacctt aataactgtt ccctgaggac ggaagctgcg atgtgccagt tggaaaggag
     6241 tatccatttt gatggtcctt tccactgctt tttcatccaa ctgcgaagaa gaaaaattat
     6301 tttcggtaac cagaacggtt ttccctgttt cctcttccag gaaataagga ttcaagttct
     6361 ctttccccag cttttccttt aattccgatt tctctaaatc gctcagcttt cgattaaaac
     6421 taacaatcaa agaatttcac ccctcaaacc aatagaacca tagctctaca agcacctact
     6481 ggatattgaa atcacgaata ataccgtcct tgatgagaag atagagtaag agataaacat
     6541 caacttttat tctccgctct attttctcct tttctttaat tcttccataa tctggtaata
     6601 ggtcatcttt tcatactgta aaagaacaag gagatgataa aggaggtcag ctatttccca
     6661 ccccaggtgc tctcgattat ggtcttcatc ttcaaaagtg gccagtataa cctcggtagc
     6721 ttcttctgct actttgcgca gaattttttc tctcccttct tccagaagac gagaagtata
     6781 agagttgggt gaaagatggg cagctctttc ctctattact tcaaaaagct cttgtaaaaa
     6841 agaagaaaga gaataaggtg gaaaatgagc ttgaggttcg gaaatttctc ctttttttat
     6901 ttcccggtga aagcaagaaa aaaaaccagt gtgacaggct actccttctt gttttacctg
     6961 aataagtaat gtatcgttat cacaatctac ataaattccc tgtacctcct gaaagtggcc
     7021 actactttcc cctttgttcc acaatttttt tctgcctcta ctatagaacc aggtttttcc
     7081 tgtctccaaa gtacgagaca atgcttctcg gttcatatat gccatcatca aaactttgcc
     7141 gctttctgca tcttggacga tggcaggaat taactcttct ttttcaaaaa ggacatcctc
     7201 ccaggatttt tccatcaatc ttctaccctc actggtattc ctcttttctg gaggtaggct
     7261 ttagcctctc ctatgctgaa atagcggtag tgaaaaatag aagctacaag agcagcatca
     7321 gctttccctt ctctcaaaat ggctaaaaga tgttccagct tccccgctcc tcctgaggca
     7381 attacaggta aacgaactct ttctgccact ttccgggtaa ggctaatatc ataaccagtc
     7441 tgagttccat cggtgtccat gctggttagc aatatttctc ctgctcctaa cttttctacc
     7501 tgttgagccc aggatagggc atctttcccg gtgggagttc gccctccatt tataaatact
     7561 tcccagtaac tacgccgatt tattcggtcc caggttcttt tcacatcgat agctaccacc
     7621 acacactggc taccaaacat ttctgctaac tcactaatta actctggttt aagaactgcc
     7681 tgagtgttaa tcgatatttt gtctgcgcca gccctgagga gattagctac ctgtccggta
     7741 gctcctatcc ctccccccac tgtgaaagga atagttaaaa cttcagctat tttttccacc
     7801 acttgaatca tggtttctct ttgttcataa gaggcggtta tatccagaaa aaccagctca
     7861 tctgcccctt cttcttcgta tctttgagcc agctctaccg ggtctcctac atcttttata
     7921 ttttcaaagt ggactccttt taccactttt ccctctttta catctaagca agggataatt
     7981 cttctggcta acactttcat tcctcccgca gaatactgca tacttcttta aaattaagct
     8041 gcccggtgta aagagcctta cccaatatta ttccctcgac accttctccc agactcttta
     8101 tcttccggat atcttcttta tgacttattc ctcccgcaac tataatactt actccgcttt
     8161 cggcgataaa atctctaatc acttccactc tcaccccttg aagtgttcca tctctttgaa
     8221 catcagtaaa gataaaatgc tcaatgcctt tcccttttaa atccttagct aaatccgcag
     8281 ctttaacagg gatttcttct ctccacccct ctaaagagac aaactgatcc cgggtatcga
     8341 tactcaccac tattttttct ctcgctatct ttatcatctc ttcaaaaact gaggggttct
     8401 taactactat gctacttaga actactcttt ctgctccggc atcaagataa cccataaaag
     8461 aagagacttc cctgatccct cctcccacct caaccggtat tccaaccgtc tgaattaatt
     8521 ctttaataac agggaaatta acgggatacc ccgattttgc tccctctaaa tcaacaacgt
     8581 gtagaaaagg agcaccttct tcctcccact tccgggctac gagaagagga gaagtgctaa
     8641 agacctctac tttttgataa tcccctttct ctaaccttac cactttcccc tccagaatat
     8701 ctatggcagg aatgggtaaa atcacttctt ccagctcctc acccaatttt ctaaaacctt
     8761 cataccccag acagaacttt tctcgggatg aaactggaaa ccccagacat tatcatgatt
     8821 cacaatggca ggaattacca cattataatt gcatatgccc accactgctt gaggttcttc
     8881 cggtaagacg taaaaagagt gagcaaaata gaaaaacctc ccctgcggta tatcccgagt
     8941 aagcggagaa ttttttttga agtatactcg attccatccc atatgaggta ttttattagt
     9001 aggaggaagt ttcaccactt cccctggtaa aagagaaagg cctttcttac gaggagcttc
     9061 ttggcttttc tcaaaaagta gctgtaaacc caggtttatt cccaaaaagg gcttaccgtc
     9121 ttcctggttt tctttaatga cttcctttat ttcttttttc tctaaatttt ccatggcttt
     9181 ctcaaaagaa cccacccccg ggagaaccac tgcctcagcc tctctaatct cttgagcctg
     9241 attagtgacc actacttctt ctcctaacct ctccagggct ttaaagatgc tatataaatt
     9301 acctatctcg taatcaacaa tggcaatcat aactatccct cttcttcttt attataaagt
     9361 ccccttagta gaaggaagat aaacactcgt taccttggca gcctgagcta aagatttacc
     9421 gagagcctta aacagagctt ctaaacaatg atgaccatta tcaccccacc atattttagc
     9481 atgaagagta atttttgcat tatttactag agcctgcaga aattccttca ctaacactat
     9541 ctcaaaacta cctactctct ctcctaaatc aggtttttcc caaagaaaat aaggtcttcc
     9601 acttaaatcg acagccacca tacttaacgc ctcgtccata ggaataaaaa cagtggcaaa
     9661 acgctctatt ccttttttat ctcccaaaca tttattcaga gcttctccca gaactatacc
     9721 cgtgtcttct actacatggt ggtaatcatc aataaattct tgagcagtag cttttatttt
     9781 taaatcccaa ccagcgtaaa aagccatact ttccagaaga tgagaaaaaa aaggaacagc
     9841 catatccatc tggatatttt tttccccatc caaggagaga tagatttcga catcagtttc
     9901 tcgagttttc cttttaacca gagcttctct ttttttcagt ctgaccactc cttactccct
     9961 attattgtaa actttccagt tcggtattag aaagaatgat gcccttccac tcccaaccat
    10021 ataagccttc ttctatcaaa agataaagaa tattataatc ggggtctggc cagttatcaa
    10081 ttttcaggtt aaaaccatca tttctttttt ccagctttcc cactcgaagg tcagtagctt
    10141 tagacaggtg ttcactaagg tagttttcaa ccttataaaa ttcatctact tcaaactcgc
    10201 taaacataac tcccacccaa aaatctcctt tcctggctaa gctttcaagc tttttaatat
    10261 ctttactccg gaaagcctct tcaagttttt gagccagagc ttctttgctt ttttctaccc
    10321 acgtgggctc agattgaaga taaactatgc gcaactctgc ccaccgacca taggaaaaat
    10381 caggtgcctc ccgaaccact ttttggtaat aagaaatagc ctcttctatc tttcctaact
    10441 tttcatagca agaagcaatt tccatcataa tccttgcttt ttcttcgcca tcggagttag
    10501 aatccagaat ttcctggtaa actttaatag cttcaagata gttcttgcta tgatattgaa
    10561 gattgtaggc tatatcttcc ctgactaaat tctttagccc ataagggtct tccacatccg
    10621 ggggataaga atctataatt tggtaaaata actccagcgc cttatcaaaa ttttctctct
    10681 cctcttctat tttggctatc caatagcgat agagataaga agatggggta ttaggaaatc
    10741 tatcgatgta cttctccaat gtctggatag ccttatcact ttctcctttt tctgagtata
    10801 tctgataaat tatttctacc gcctgagaca gttcttcttc ggaaaaagaa tctagaagag
    10861 gttcgagaag agcaagaact ttatcagcct ctcctttttc taacaaccac tgagcctggt
    10921 ctaagggctc caaagataag acaattcctc cactatttac caggtaaaca acaaaaagag
    10981 gaataaataa gggaataatc ttcgattcca aaatttttct gccctccctt tccttttaat
    11041 ttaaagaaga agttcataaa aattatgatg tactttacta tctattccga ttttattaaa
    11101 caaaagtcta atattgtaaa aattcaaaaa ccaaccttct tatataccaa gtctttaaat
    11161 ttctgattaa aattagcaat gctgcgggta gtggaacaac ctttttccaa aaaccaagac
    11221 caaaataaag catattgaaa ctacaacagg aaaactattc tagtggacga aaaccctgat
    11281 gcttctgatt ttttaattta ctaaaatgaa atatgtcttc caaacagatc tttttatccc
    11341 ctggtttatt ttagcgatta aggatagtat aatcataaca tgtttttaga aaggagtata
    11401 gaaagaaatg gaaacatttg atttttggcg agaactaaag aagaattgga accttattat
    11461 tcgttaccct cagatcattc tcgttaccct tattccctct ctgttgattg ccacctctaa
    11521 ctacctgacc tggcaaggaa gtcttacctg ggtttctgtt tttttccaag aagcaggaag
    11581 cgcgatttta ttaattctgg gagttttcct gagttttatt gccctgagtt tagtaatagc
    11641 aatagcctgg gattaccgct atcgagatgt gatagactta cgtagggcct ggcggatagt
    11701 tcaaaagcgc ttaccggata ttgtaatggt ttccctcata atgggtttca tagaaggatt
    11761 ctttgctatc tggttcatgt tcttaggact tgtatttgct ttcctgttgc tctttactat
    11821 ccctctcaca gtggtcgaag gagataatcc tttttccgcc attcggaata gttttcacct
    11881 tgttagcgag aatttaggag aatgcttcac tttttttatt atcgctctat tttttctcct
    11941 gataggctat cttctattct ggcttttagg ctttttagga atagcaggac taatattaaa
    12001 tactgtagta ggagcactaa ttctagctta tgcttctctt ttgctttcct ctttttattt
    12061 ttccttaact cgtttttaat cttttagtgc ttcttccaag tctattttca tgccatcact
    12121 ggcagctatc acttttatgc ctgttttttg agacatttcc tcggctatct tccagggttt
    12181 ctctcgtaaa accgttaacc caaaatgagt aagaaaagcc aggcgaggct tgatattctc
    12241 aataataact tgagcatcag gaatacttag atgaagtatg gaggagtggt tatctttttt
    12301 aaagcgtaca gtatttagaa acaatatttt gctatcttga taagcttcga ttaatttttc
    12361 ctcaaaaaga gtatcagtaa taaaagacac atccccctga ggaaaagaaa aacgaaaacc
    12421 ataggtttca gcagaatgca agtgacgcaa aggagtggaa aaagaaatgt ccttccagtg
    12481 atattcttta ccttcttcca gaatttccag actcaccaat ttttctcgca aatatttaaa
    12541 caacactggc tccccctcta aagcttctcc aggaagaaaa acagcccccc tctttttggt
    12601 ggcgccctcg gtcatagctt ctaccataat attgaggtca ttggagtgat ctaaatgacg
    12661 atgagaaaga agaagagcgt ctaaagatgg aggagaaaga ggaggacgac tggacagagc
    12721 cctaaccaaa gccccaggac caggatcaat gtgaagattg gtatcctgat attttatcca
    12781 cactcctcct gaggcacgta gttgtttaga aactactact cgggctccag ccgttcctaa
    12841 aaatttgata aaattatcca ttataaaata gtccgctcca aatctatctg actcttcata
    12901 tctatttcac caattatagt taaagttaga gaataagaaa gatcaatttc ttctgagaag
    12961 tatacatttc cctgttcgtc ctgtttttta atacgaatta caggtctaaa gggaagaaca
    13021 gggttgattt tgcttaccat cgcctcttct ccagtggata attttatcaa atctcccacc
    13081 tggtagggag aaagatggcg gataaaagtg gttaccacct catcatcaag tttatgacca
    13141 gcgcttccca ttaaatattc aataacctcg gggataggaa gagctttacg gtaaggtcgg
    13201 tcagaagtta gcgcttcata gacatctgct actgcagtaa tgcgggacaa aacagaggtt
    13261 tcttctttgg tcaagcgaag gatataacca ctcccgtcta ttctttcatg atgctgcaag
    13321 ggtatctctt taactctttc gggtaaccgg gtcttctcat ccaaaatatt ttttgcaaat
    13381 atagtatgtt tctgaataat ctcataatct ttttctgaga gcttagaagg tttatttaaa
    13441 atttctgggg gaattagaag ttttcctaaa tcatgtaaaa gagcacctaa acccagatgg
    13501 atgagctctt cgtcttttaa tcctaaaaat ttagcaataa acaaagaaat aacagctaca
    13561 ttaagagaat gagtaaaagt aaggtcatca tgtttcttca gttgtataag agggataacc
    13621 aactcttcat gagctttgac ttcctttgcc atctgagaaa cattctgttc taagggcaca
    13681 atagaagggg tttccccccg ggataaatct tctaaaattt cctgcatatt ctccagggtt
    13741 ttctttttaa tttccggaga tatatcttct ttagctacca atccttcgtc ttcttctaaa
    13801 atctcagcta aaactgaaga atagccatcc tctttgagtt tttctacaaa ctcagaagtt
    13861 ataatctgcc ctcgactcaa aagaacctga tcctggggat taagaagagt aaaagggaga
    13921 agctctcctg cttttagctc agaaagggga atcttgactt tttttattcg ataggcatct
    13981 ttcaattcta tcctccacca caattataat aattttccct ccggcagttt gtttaaaaat
    14041 aaaagacctt aaaaaaaacc ccatatttta taacatctca aatctgaagt tatactattt
    14101 ttagttgaaa ttgaaaagaa cctcagcgaa agcataatgg aacacggaaa aaaatagcta
    14161 tgtactgaag agttcagggg gtaaaaagga gaacgaaaat ctgtagagag ggggagatac
    14221 ccggactttg cactgcaaat ggcagaataa gcctacacat gcagtgccag agctttagca
    14281 gtgctaactc cgatataaaa gagtttagat agttatctta gattctatta catagatgca
    14341 tagatagctt caataatccc cagaacacag aacaatacga catttacttt gagaaaaact
    14401 gtaaataaac ggggcgatga aacaatctac ctttaaaatc tctttttgta ctgatgttga
    14461 gagaatgcct cggtcatcac cttttttctt gatcaaacgg ttgtttttca gtcttgaccg
    14521 aacaaaaatc aaagaatgga ttagcctgcc tcctccgggt tggttttact tatacaaata
    14581 ctttatccgg aaaagcaagc agtgctcgtc tttatttaat cctcccatta aatgtttagt
    14641 agaagaacca caaaataaaa aaaacgaaaa acaaaggcta aatagagaaa tgcgccctgg
    14701 tgtgcttaca acgctaaaaa cacattttct tatgaaggga gaagtattat gtgatttttg
    14761 tggctaatta caaatagcta tacacctatc atcgcgaagc tttaataacc tggtcaataa
    14821 ttttgagaaa ttctctattg gagcgagtgt ttttcatccg ctcaataacc atctgagttg
    14881 cttcttgagc atctacagta gacaaaagct ttctcaacat ccatattttt tctaaatctt
    14941 ctttctttag caagagctct tctctccggg ttcccgaacg atgaatatct atagctggaa
    15001 atatacggct ttccgctaaa gcacggctta agtgcagctc catattacct gttcctttaa
    15061 actcttcata aatcacctca tccattcgtg aaccagtatc aaccaaagca gtggctataa
    15121 tggtcaggct accaccttcc tcaatgttgc gagctgctcc aaagaagcgc ttgggccagt
    15181 gaagagcgga agaatcaaca cctccagaga gagttttccc cgtagtagga accaccaggt
    15241 tattagctcg tgccagacgg gtaatactat ctaaaaggat aaccacatct ttaccttcct
    15301 ctactaaacg tttggctcgc tccagagtaa gttcggccac tcgcaaatga ttttctggct
    15361 tctggtcaaa agtggaagcg attacctgtc ctttgactga gcgttccatc tgagtaactt
    15421 cttctggccg ctcgtcaatc aaaagaacaa ttaataccac ttcaggataa ttggccatta
    15481 tgccgttggc gattttctcc aaaagcaccg tttttcctgc tttagggggt gatactatta
    15541 atcccctctg tcctttccca atggggacaa aaaggtcaat gatgcgggta gaaatctcct
    15601 cgggaacggt ttccaaaact aactgctcat caggaaaaat gggagtgagg ttctcaaaaa
    15661 gagggcgcct tttggcttgc tctggatctt catcatttat agcctcaatc cgcaaaaggg
    15721 catagtatct ttctccttct ttgggggggc gtacctgtcc cgctatttta tctcccacac
    15781 ttaacataaa gcgtttaatt tgtgaaggag aaacgtaaac gtcgctatca ctggggctga
    15841 aatcatcaga agtacgcaaa aacccataac cctcaggagt tatttctaaa atcccttcac
    15901 tgaaaagata acccttagac tcagcagact ttctaagaat ttcaaacact aaatcatttt
    15961 tcctcttacg actgtaacca gatataccca agctctgagc aatctcaaac agttcggcat
    16021 tagttttact tttcaggtca gctaaggaaa gttgcatagc gggtgctttt ctatttgttt
    16081 ctttctcttc cactaactct tttacctgat tgttatcact atccacttgt atttcctcct
    16141 attatttttt taaaattctc tccgctacct gaacatgtcc taccttgtct acatcaaatc
    16201 ccatttcagg gtagggagta ataacggcca aggcttttat gcccaacatc ttgtccactc
    16261 gctcttctac atccttgata gttaattgct tggtaagata tttaaaaatg acctttgctc
    16321 ctatcaatcg agcaagagca acagggtttt tgcgactttc aactattcga gaaagccact
    16381 cttttttctt ttccatcatc tgaggattta gaaagagaat atcgccgcct gtaaagcatc
    16441 catctcgcaa acgtacaaaa gtcctctgag aatcatttag cttcttttca taaatttctc
    16501 ggcggatgat gggataataa aaatcagctt cttctttact gcatctcagc aaaaaatctt
    16561 ctaccatttc accttttaga aggggcaggt cactaccagt tattagaact ttctctctgg
    16621 tttgtaaagc ttccaaacct ttccaggcac tttcgaaagg gttttcccct ggtggaacaa
    16681 cttcttcaac taaactacca attcggcact ttatttcttt gactggccca actactataa
    16741 ttctctttat agaagaaaca ctcttgaggg cttctattac atactctatc ataaacttat
    16801 cctttatgac aagtagggac cggttaacaa ctccaaattt tttttcaatc tccccttctc
    16861 cgccaccagc taaaactata gcgtcagtca ttgatttgct ccctcctctt ttgcctccca
    16921 tatggtagaa gttaaaatag ctttaacatc aggagaggaa atttctttca ctattctctc
    16981 accttcttct ttgctttcta ccacccccac gagagtggag ccactaccac tcataaaaac
    17041 ttttttacat cctaactcca ttagcagtct cttatagaaa tttaaatccg ggaaacattg
    17101 aaaaaccact tcttcaaaat cattccaaat tatttcctct attagctctg gattcttcgc
    17161 tttcaggaat tcttcaaggc ttaacccggc ctttttattt tctttaattc ttccttttct
    17221 ctcctcccag caccggtaag cccaggcagt ggaaatagaa aaaggaggga aaagcaaaac
    17281 taaaaaccgg tgaggagcaa aaaaaagagg ctccacttcc tctcctcttc ccctcaccca
    17341 ggcaaaagga gctccccggg cgaaaaaggg aatatctgag ccaatacgaa cagcgatgga
    17401 tttaacttct tcccaatcca ggtttaactt aaaaaccgaa ttaattaatt gtaaaataga
    17461 agctacgtta ctgcttcctc cccccaatcc acttcctggg ggaatacttt tcttaatctt
    17521 tatgttaaaa ccgacttctc gaagaggagg agctatttta cgtaattcta ccagcaatct
    17581 actcaaaaca ccatcttccc cggtgggaat gggaaaatta caggcaatat aatctctttc
    17641 cctttcctct aaggaaactt caatttcatc gccaaaagca attttctgca ttagcgattc
    17701 aatttcatga tagccgtcgg agcgtgtttc tccaatcttc aaaaaccagt taatctttcc
    17761 gtaagaaggc aaaataaatt tttcagacac tgatattcac gcctaaatca acagggaaaa
    17821 ggcttacttt ctctttcaaa tgaaatgaca aattatctaa agactcctga aaagaaaaac
    17881 cgtcgcctcg caccaaaata gatagtttgc ctgaagaaga gctcctcttc tcctcagaat
    17941 ataatttatt ttttacctct tgagcacagg cccaggcagg gtctaccact tgtagctccc
    18001 tcccccccag ttcttgaaaa aaccagctga taagggaaaa atgggtgcaa cccataatta
    18061 gggtgtctac tcctctatct tttagagaaa taagtctatt tttaatttgt accctccatt
    18121 ccgggctatt gaattctccc ttttccaccg cagcaacaaa ctccggccag gcttcctcaa
    18181 ttacctccaa accaggatta acccccttta aggtttggcc aaaagctcct gctttcactg
    18241 tagcccgggt agccagtact cctatcttac cctgccgcgt tactctcgct gcctcctgac
    18301 aggccgactg gacaattccc ataattggaa tgcttatcag ttgagctaaa tcaggaagac
    18361 aaacagagct aagagtgcca caagcggtaa tgattaattt tgcttttctg tgctgagata
    18421 gatagccaat aattggcttt actatttcca ctaactcttc tctacttttc tccccataag
    18481 gaaagtgaag agggtcggct acatatataa tatcttctgc cggtaaaaga gccctcactg
    18541 cattaactac gcttaaacca ccaatacctg aatcaattat tcccagaggg tatttaaatt
    18601 ccaagcctat ccccctttct ttcttgtcga tagcaatgag ctgcctccac aaagccacga
    18661 aataaaggat gagcttgatt gggtcgagac ttaaactcgg gatgaaattg cactcctatg
    18721 aagaaaggat ggtcctttaa ctctattatt tctaccacct gataatcagg gttatagcca
    18781 gccatccgta gtcccgctct ttccagttca tctacaaaca ggctattaag ttcataacga
    18841 tgacgatgtc tttcccaaac ttcttctttc tgataaagag atttacttag agaactctct
    18901 aaaacacacc gatagttacc caaacgcatg gtcccaccca ttttttgggt atctttttga
    18961 gaaggtgaca aatcgatcac cgggtaagga gttaagggat cgaattcggt agaatgagcg
    19021 ctatgaaaat gaagaatgtt tcgagcaaac tcaatcgttg ctacctgcat tcccagacat
    19081 attcctaaaa agggaatctc cttttctcgg gcataccgga cagcctgaat tttaccttct
    19141 attcctcgct gaccaaaacc tccagggata attataccat ctactccaga aagaaaacaa
    19201 cttgcctcat caccctccag ttttcccgct tctattggtt taacgctcac ttttaatccc
    19261 agttctatcc ctccatgttt aagcgcctca caaacactca agtaagcatc ctttgattct
    19321 gtatactttc ccaccaaacc aatatttact tctttatccg cttctctaac cctttgaacg
    19381 atttgagacc attcctctaa atcagaagga gtaggcttca tttccaagcg actggctatc
    19441 aggtccccta catttctttc ttccagaacc agaggcacct cataaatgga atcaacatcc
    19501 ataaggggaa tcacggcctc tttatccaag tcgcagaaga gagaaatctt agccatcact
    19561 tcgggggtaa taggatgaga agaacgacaa actattatgt ctggctgaat accaatactt
    19621 cgtaattcct taacgctatg ttgagtaggt ttagacttta gctcccccgc tgcctcaaga
    19681 taaggcacga gagtcacatg aatataaagg caattgcttt ttcctatatc attttttatc
    19741 tggcgaatag cttctaaaaa aggcaaaccc tctatatctc ctactgtccc tccaatctcc
    19801 acaatagaga tatccgcttt gctcatttct ctcaaccgaa aaatctcttc ttttatctgg
    19861 ttggttatat gaggaattac ctggacagtg gctcccaggt aatcacccct tctttctttt
    19921 tcaatgactg aactatagat ttttcctgtg gttacattac ttagtttgga tagctcctca
    19981 tcaatgaacc gctcataatg tcccaaatct aagtcagttt cccctccatc gtaggttacg
    20041 aatatttctc catgctgata aggattcata gtaccagcgt caacattgat ataaggatca
    20101 aacttctgca tagtgactcg aaatcctcta ctctttagaa ttctccctaa tgaggcggcg
    20161 gttatgcctt tccctaaaga cgatgctact ccaccggtta caaaaatata ctttccttta
    20221 tccattttta actcctttta ctcaatatgg tatcgtcaaa ttttcatctt aaggagaaaa
    20281 taaatctctt taactgagaa agaaatttag ttattttaca tagtgtcagg agcggaaatt
    20341 cccaaaagtt gaaaagcatt cttcagtaca atttgtaccg ctctacagag acttagccga
    20401 aaagaagtaa gttctatatt gtcttcatcg attatccgat aaaggttata gaaagagtga
    20461 aaatctccac acaaatcctg aagataatta caaatcaaat agggctgacg caaaagagca
    20521 ctacgtttca ctgtttccgg gaaataagct atttttttga cgatttctct ctcctcttca
    20581 gtgggaaatt cagacaaagt atgagtcaaa ggaaacgatg taaatcctct tttttctgct
    20641 tctttaaata aggaagaaat acgagcgtgg gcatattgaa cgtaatatac cgggttatcc
    20701 atagattgtt ttttcgcttc ttctatatca aattccagat gagtagctgg atctctcatt
    20761 aaaaaataat agcgggccac atctactcct acctcgtcaa tcaactgcct caggggtaca
    20821 aactcaccct gtcttgtaga cattctttct ggttgccctc ctcgcaaaag agtaacaaat
    20881 tgcacaataa aaatttccag aaaatcttcg ggaagaccca gagcacacat cgctgcgtgc
    20941 attcgaggga tgtagccgtg atggtctgcc ccccagatgt caataaccag ttgaaaccct
    21001 cttttccatt tattccaatg ataagcaata tcagaagcaa agtaagtagg caatccattt
    21061 tctctcacta atactctatc tttgtcatct ccccaatcag tagtccggag ccacagagca
    21121 ccatctttat tatagacata tcctcgctct ttcagaagag ataatatttc ttctgcttct
    21181 tttttttgat ataaagaact ctcccggaac cacacatcaa aatgcacccg aaaatcagct
    21241 aaatcacggc aaatattatc cagaatcttt tgcaccgcaa acttctgaaa tacttctgaa
    21301 cgatccgtag gaggaaggtc taaaactcca cttccttttt cccgtaataa ttcctccgca
    21361 atagggatga gatactctcc ctgataaccc tcttcgggaa cttctacttt ttcccctaaa
    21421 agctggcgaa agcgagcctc cagagaagct cctaaaaggt caatttgcct ccccgcatca
    21481 ttaacataat attccttttc cacctggaaa ccagcttttt ttaagatacg agacagactg
    21541 tctccaaaag cagcgcactt gccgtgaccc acatgcagag gaccggtagg attgacactc
    21601 acgaactcca cctgggtctt ctttcctttg cctaagttca ctgctccaaa atcttcccct
    21661 tctccctcca cttcctgaat aaaggataat aaaatttcat cctctaaaaa gaagttaata
    21721 aatcctcctc ctgctacctc cactcgagca aaattctgca aattccggga gagagagagt
    21781 tgtaaactgg tagccaattc ccgagaaggc ttcttcactt tccgagaaag aataaaagcc
    21841 aaattagtgg caaaatcacc atggctctta tcacgggtag gctcaacagt aatttctttt
    21901 tcatccacca cctctggaaa atccaccgcc agaacttttt ttatttcctc tttcagcatt
    21961 tcagtaatta agcgactcat agacctcttc cttaaaaaaa tcttaaattc aactctggtc
    22021 attatatatt tttttaaacc agggttcaat gggtatttta tggttgacag cccgtggcgc
    22081 aaaaaagtaa atgacaatta taatatttta cccggttaac acatgctttt atccttgtta
    22141 atttcagaaa ctataagggc gaagtatccc ttttatgatg agaaaatttt cccctcggtg
    22201 atttttgtat tttatgtttc tgttttgctt ttccagagag taacaggggg tgtgtttacc
    22261 ccctgtagtt atgttatact acacgtaaat taaaaaaatt agttgcttca ttttactaaa
    22321 taaaggggaa aaaagaatgg aacaattttt gggcattgat ttaggaactc agggagtcaa
    22381 agtaataggc tgggatgaag agggtaattt tttaggctcc accagccgtg aataccccat
    22441 tttaactcct caaatcgggt ttgcagaaca gaaccctgaa atttggtggg agaaaaccaa
    22501 agaagcaata aaagaattag tgaacttgat tcctcaagtt aatgttaaag ccattggttt
    22561 ttctggccag atgcatggaa cagtaataat agatgataac cttaaacccc tttatcctgc
    22621 tatcatctgg gcagaccagc gcagtcacca ggaagtaaaa atcattcgag aaaaattgga
    22681 agacaaacta gctactatct gcggaagcga tgtagctacc ggttttatgg caccaaccct
    22741 attatggctc aaagaaaacc agagagaaat cttcaaccgc ataaggtggg caatcttgcc
    22801 taaagattat ttgaaaatta aaatggggct taccccttct actgatgtgg ctgacgcttc
    22861 ttccactctt ctttttaaca ttcgaaaaag aaactggtcg gaagaagttt tagaaatttt
    22921 aggattaaac gataacattt ttcctccggt ttatgaatcc actcaggtaa tcggtcagat
    22981 gaaagaggag gttaaagaag aattaggttt aaagggggag gtccttttag tagccggagc
    23041 aggtgaccag cattgcgctg ctctgggaaa tggtataact gaagaaggag agcttttaat
    23101 aaccatagga actggaggac aggtatttac tcctctctgt gaacctctgg ttgatgataa
    23161 attgcgtatt cacactttct gtcatgctat ccccgggttc tggcatcttt taggtgccac
    23221 tctttgtgct ggtcttgctc ttagctggtt taaaaaatca gttcttgatt ctccgcttca
    23281 ggcttttgat tttaaagaat tagaccagga agctcaaaaa gtggaagctg gatcacatgg
    23341 tttacttttt ctcccttatc tcatcggaga aagaactcct tacatggacc cccgagcccg
    23401 agggtccttt ctcaacctcc acctcaccca cggaagagcc catttcattc gtgccatcat
    23461 ggagggagta tgtttaggat taaaaaatgt taccgaagtg tttaaagagc tgggagtaga
    23521 accgcaacgt atagtatttt ctggaggagg agctaaaagc gccttatgga gaaaaatcat
    23581 ggcctcagtt cttgatgctc cccttttcac cacccaggtg agagaagaag cagctactgg
    23641 cgcttgcatc ctggctatga tgggaacaga aaaatcttca gaagaagaaa ttatttctaa
    23701 aataatccgc tatggaaacc ccgttctccc ggagagaaaa gcagcagaaa tttatgctga
    23761 aatataccct aaatttagag atgcctaccc tgctttaaaa aagctatttc cctaacagga
    23821 gaaaattatg agagattgga aggaagaaac acaaaaaatc attacctgga tgagaaaaaa
    23881 agtagaagaa gctaaagctc aaggattggt actgggaatg agtggaggca ttgattcgtc
    23941 agtggtagcc gttttagccc agaaagccct gggagaaaaa tctctgggaa tcatgatgcc
    24001 ttgttacagc ataccccagg atttagagga cgccctgaaa gtggctcaga tgttctctat
    24061 tccccaccga gtcattcctt tagaaaaacc ttacgatgct ttcttagaag ttttaggaga
    24121 aaaacaagaa aatagccgtt ctttagctct ggctaattta aaacctcgcc tgcgtatgtg
    24181 taccctttac tattttgcca accgattaaa ttatttagtc tgcggcgctt ccaataggag
    24241 cgagataacc gtaggctatt tcaccaaata cggtgatgga ggagtagata ttttacccat
    24301 tgctcacctt accaaaaccg aggtaataga attagctcgg tttctcaacc tcccttcttt
    24361 tatcatcacg aaaccccctt ccgcggggct atgggaagga caaactgacg aagaagaaat
    24421 gggaatcact tatgaaaaaa ttgatgagtt tatccttcat aaaaaagtgg aggaacccta
    24481 tctttctcag ataaaaagca aaattcgagc aagcgagcac aaaagaaaaa tgcctgccac
    24541 accagaagaa attactattt agcttttttc ttgcaattaa ggaattccag tagtttttct
    24601 aagctaaagg tattcagcac gtcttctttt cgcaaccagc cccgacgagc ttgggctact
    24661 ccataacgaa tataatccag ggaagaaggg ctatgagcat cagtagaaat aaccaatttc
    24721 actcccaggt ctcgagctcg tcgagcatct acgtcactca aatccagacg ttcaggctga
    24781 gaattaatct ctaaagaggt tctggtttct ctggccagat agaaaagttt ttctcgattt
    24841 acctgatagg catctctcct gttgaggata cgcccggtag ggtgactgat tacctgaaca
    24901 tatttgttct tcattgctcc ttccagacga cgcaagattt tttcttcccc ctgtcctaga
    24961 ccggaatgta atcctgccac cactacttct aacttttcca gttcagaatc aggaaaatca
    25021 agagaaccgt cgctcatgat attgacttct accccgtcta aaagtcggag tcctttccct
    25081 tttttattcc atcgctcgat ttcctttctt ctttctcgaa tctcctccgg gctaagaccg
    25141 gaagctacag ctaaagattg agtatgatca cagatagcca gatacttgta accccgggaa
    25201 agagctgctt ctgccatttc ctctatggaa gctactccat cactccagtt agagtgagcg
    25261 tgcaggtctc cctgaaggtc accaatctcg ataagaacag gtaacttttt ctcctgcgcc
    25321 agttcaattt ctccctggtc ttccctcatc tctggaggaa tccattccat tcccagagtt
    25381 tcataaattt cttcttcctt ttctcctccc actcgcttat cgttatccag tcggaatatc
    25441 ccatactcat tcactttaaa tcccttttta agagcaattt ctcgcaattt aatgttgtga
    25501 gctttggagc cagtaaaata ttgcaaggcc gctccaaaag atgtttcttc taccactcgc
    25561 aaatccacct gtatcccttc ctgggtaaca atactggatt tagtagtccc ccgggcaatt
    25621 atctctcgca cctgaggtag agaagtgaaa atatccatta tagattcagg agtagaagaa
    25681 gccaccagaa tatcgatatc tccgatggtt tctttcatcc ggcggatact tcctgctggg
    25741 ctgattttag tcactccgct tttttcctga ataacctcta ttatttcttc caccaaagga
    25801 agagcctgac ccagaagcat tctcccactt tgcctcttaa gcatctctat acctttccgg
    25861 attttttctt cactttttac ccccaagcga ggaagatggc gcaatttttg ctcagcgata
    25921 gctttttcca aatcttcaat gctttttatc cccagctggt cgtagagaag cttagcaatc
    25981 ttaggcccca ctcccggtat agcagtaaga tgaacaactt caggagggat ttccttggtt
    26041 aattcttcca aataagatag cttaccagta gaaagatatt cttcaatttt actggcgata
    26101 ctctccccta ccccgggaat ctctttcaat ctcccttctt tccacacctc ttcaataggc
    26161 tcaggccagc tttctatgcg gcgggccgct tcttcgtaag ccactatttt aaaacgattt
    26221 tctcctttca tctccaaaag agtagcaata ttttctaaaa gttgtgctac ctcccgattc
    26281 ctcatacacc ctttcctcct ttctcttaat atgctatcat taaaaagcga tgagggtaag
    26341 gaatttatct tttttggggc tgtttttaat cgtaggaaca gctatttttt tcaatgttag
    26401 tttaagctca gctaatgaaa ctgatttata tgcgcttttt cggcaaggag actatgaaac
    26461 tataatttct cttaagcctg aagaaggaga agagcttttt ctctatctcg tatctctcct
    26521 caaccttagt cgagaagaag aagtagaaaa agaaatttcc tccctgtcgc cagaaaaaga
    26581 ttatttctgg ctagatcacc tcttttatct atcttctctt cactgtatag aaaacggaga
    26641 cttagagaaa gccgttatat ggaaagagag attggcaaac tcctttccct cctctcccct
    26701 tttaccttat atcactttac gtttagctca atcttttttg gaaaacgact ttttcagttc
    26761 tgctcttttc tattccattc aagttcttac ttattcagaa agagaggaag acaggctatc
    26821 agccttgaaa ataacatttc aatctttagt taatctcggc tacctcgaag aagctaccct
    26881 tttactctgt cacatctatt atgtttttcc taccctgagt tctcgagaaa ctcgagaact
    26941 cattttgcct gtgcttcccc agatctttcc agaaaaattc tttccctctc agcgtctttc
    27001 tttagctaat ttcctctttt ctctgggttt tactgaaaaa actaaagatt tcttagacca
    27061 gatagaagag tttcttcccc cctcccagca ggtagatata ttcactcttc gcgcccggat
    27121 tttcgtcaga gaagagaatt gggaagaatt ggaacagcta ataagtaaaa atttattaaa
    27181 aagaggagaa acagaagagg ttctattcta ctggggagtt ttagaacaga gaaaggccaa
    27241 ttatggaaga gcagctcaaa tttatgaaag gctgttattt ctttaccccg agagcggata
    27301 tgcttttaat acttaccaga atttagcttt ttctctccga gccatgggag aagaaggaag
    27361 atatttagaa acaatggaga agattatttc ccgtttccca gaaaagagcg cacccgtctg
    27421 ggaattattc tggttctatt acaacagaaa ccagatgtat tcagcacaag aaatgttaga
    27481 aaagctaaaa agtttttctg aagaaaaaaa taaagcactt ttctggtcat ttaaaattga
    27541 ttcttcttta gaagatttag aggaaatagt ggaaggcggg aaaatagatt actattatgc
    27601 ccgagcctgg caagagctgt ctgacagagg agttgtcctt cctttccctt ttgatgagga
    27661 agttcctctc cctcaggtag acccggcaaa tatttcctat tctgaacatt ggacaaagta
    27721 ccagtttctg aagaaaatag agatgtggga gaatgctgag atagaaattc tatttcttct
    27781 ctctaagaac tctcaaaaga aaggctttta tcgagaattg aatgaattgt atacccaaaa
    27841 aggagattat caaaaaagca ttctctattc tctttatctt caggaaggag aaagaattcc
    27901 tctcaattta gcccggcaaa tttacccgga attttttctg ccttctattc agaaactaac
    27961 tggagatgag ttagaccctt acctgattct tgcccttatt cgagcggaga gttttttcga
    28021 ttcagcagct atttcctctg ctggagctat gggattggct caagttatgc cctctactgc
    28081 ttcatgggtg atagaaaaag gatgggcaga tggaggagga gaagaggata tttccattct
    28141 tcttctaaat gccgaaaaaa atttagagat tggagtgagc tatttttcct atctctggaa
    28201 tcgttttgga ggaaaactct atcccgctat ttgctcttat aatgccgggc cgggcagagt
    28261 agaccaatgg ttagaaactc tctccaccga ccctgacctt tttgctgaat ctattccctt
    28321 ttctgaaacg caaaattatc taaagaaggt gatggctaat tactttgcct actctatcat
    28381 ctaccagggc aaatttagcc ctcctcctta tctttagctc atctttaaat attttttgaa
    28441 ctgctctatt ttttcttctc cttctaagat gccaatttcc agagaaattt tcttagattc
    28501 cccgggttga agagttacta atctcccctc ttctctttcc cggcgcctac ccagaggaaa
    28561 acagttgccc ggctccatac ctaacacata ttctccttcc cccatcatct tccattctgt
    28621 aaaataagga atggtatctt tgctaaactt aacataggcc cccattttaa gccggtcatt
    28681 caccaataaa gaggcaccat aaccatcttc taaagtttta ggataatgga ggtaaacttt
    28741 ttcaaaatac cctttttggg gagaagaaaa aagatgccat tcttcttttc cttcttctgc
    28801 catctcatct cgaggcacag ttttctccac cgggaggaat aacctggcac cgtcatccaa
    28861 caaaggatag ccaaagttga tgtggtataa aatcataaag gggctctcat tccagccctc
    28921 gttaaccacc tggtcttcaa tatggatagt tgtgcctcct aaacggctcc agatttttcg
    28981 gtagagagct attttatctc caaacactat agattctttt atctttccct gcacccacat
    29041 taagtaatca tccccttgcc aggctccgtc tacccacaga ttactggccg gaagataaga
    29101 ggctctacca tgcaaaccaa aatcttcttc ctctaaaaaa ggatgattag acttgtctcg
    29161 tcctgcagcc ccagcataag tcaggccaca ggtattcatt aaccccccat gaaatccccg
    29221 caaccatccc aaaccttcgg gctcaaaaaa ggcaggagct aaattccgag tagcggaacg
    29281 ccaactaaca ttcatgcctt taaagctggt ccaggcaata tccatccccc ggtccaggag
    29341 aacagtataa gtcaaacctc caccagagtt aacctcagct attcttaccc ctctctctac
    29401 accttcactt aattcagcaa gctttacccc tccaatttga gagatatcgc ctactctttt
    29461 taagatttct tccctggtaa tttcttttcc ccataaatta gccatagcta tttccccctt
    29521 aatttatttt tattccgagt cgtttctacc atctgagtga aaaaaatggg atattctatc
    29581 tccgggtctc ttttcctctg atagtttctt tctctgtgcc tcccgcaaaa aagcaggaac
    29641 atctaattca tcctctataa tgattctact atctatctca tctattgttt cttctatctc
    29701 atctaatttc tcctccggaa gctcaaaacg ggtagcaata accgttacca ttgtttcatt
    29761 agacagactc tcatccaccc gataacccca caaaatatca gtttctgttc cagctgcgtt
    29821 ggtcacagta ttaacaattt cagtaacctc actgagagtc atatcctgac caccggtaat
    29881 attaaggatg atagacttgg cacctttgat tgagatttcc aaaagaggac tttgaatagc
    29941 ttcttctgct gcttgcttgg ctttttctct tcctcttcca tgacctattc ctattaaaac
    30001 catccctgct tcgctcagaa cagttctcag gtcagctaaa tctaaattaa tctcctgagg
    30061 agaggtaata aggtcagtga tacctcgcac tgcctgataa agaatatgat ctgctattct
    30121 gaaagcttct tgtaggggag tatctttttt ggtaatctgg aaaagtcgct cattgggaat
    30181 tataatcaaa gcgtcaactt cttgttgcaa tcgactgata ccttcttctg cttgtctttt
    30241 tctctttaga ccctcaaaat caaatggttt agtcaccact ccaatggtta gagctcctga
    30301 ttccttagat atattagcga gaaccggagc tgctcctgta cccgtgcctc ctcccatgca
    30361 agcagcgaca aataccaaat cagcattttc caccagctgg cttaatcttt ccttatcttg
    30421 tcgggctgct tcctctccca ggcggggatc cccccccgtt cccagacccc gggtgaggct
    30481 taaaccaatt tgtagcttct cttgggccag agattttctt aaagactgaa catcggtatt
    30541 cactgctatc aagtctactc catctaaacc caggtcagcc atatgattaa ccgcgttatt
    30601 gccccctcct ccaattccca ctacttttat cactgctagt ctgtcttctg cttttttctc
    30661 tatctttgcc ttagttttct ccagattctt tttaactgcc tcaccagaag ctttttgctc
    30721 ttccttttca gggatagagt cttcctcccg tatgatacct tttgtggtat cccgaattaa
    30781 ttctctctta aaatcaattt tttcggagaa gcctctttcc aggcttttct tcttcaagct
    30841 tgatcacctc cttggctaag tttaagtaac actgagctcc agaagaagaa ggttcatatt
    30901 ccataaccgg taccccgtgg ctggtagcct cagccaggga aatattacga tgaataacag
    30961 tttcaaaaag atgatctcca aaatattttt ttatctcttc taccacctct cgataaagat
    31021 tggtacgaac atcggcaata gtaataagaa cataaacttc caggtcatgc ttcaagtttt
    31081 ctttcatcaa gcctatagtt tccataaact cttcaattcc cctcatagca aaataatgag
    31141 gctgaatagt gataatcacc tcccgacagg ccttaagagc attaacagtt aaaattccta
    31201 aggaaggggg acagtcaatg aggatgtaat caaaagagag attagctctt tgaataccgc
    31261 tatttaaaaa gtcttccctg cccagtttat caatcaagcg atattctgct ccagctaagt
    31321 caatattggc tggagcgaga tagaggttgg gagtgcgagt aggaataata acttctttaa
    31381 gctcaaggcg gtaagggctg cgagggggct ccagtacatt gagaatggtt ctgtcaaatt
    31441 catcaggctc aaaacctaaa cccagagtag aatgagcttg aggatccata tcaataatga
    31501 gaactttccg cgcatgataa gccaaagaag cccccaaatt tatacaggtg gtggtcttcg
    31561 ccactcctcc tttttggtta gcaatggcaa ttattcgcat agtattctct cagaaagttt
    31621 attcctttta taatttccac tatgatatca tatcacaatt tagagcaaat attcggtaat
    31681 aaaatatcat tagtaaccaa aaagttagat agataaatac taccagaaat agaggaattt
    31741 ccaatcccca gaacacagaa aataaatgaa caaccaggga agagattacc gataaaaacc
    31801 cattccaggc aaaaacctgt ccaaaaaagc ggggatgaaa agtagaacat agattagaga
    31861 gcaagggaaa aggtaacccg caaagatagg ctacaccagc taaaggtaaa aaagtaacca
    31921 gcaagctccc ccagatgggt aaatgaagta gcttctcctc taaaagaagc aagaatccag
    31981 aataagcaaa aagaactaaa ggatgtaacc aggctaactt tttgacttct cgaaaatcca
    32041 atccccgtct catgcgccag cttcctaatc cagaaaaaac caaaagaacc aaaagaagaa
    32101 gggaaaaagt gtaaaaaggc aaacccacta tcatctctaa ttttataaac agggaaattt
    32161 ccatcatcat aaaacccaca ccggtcaaca ttcctcctcc tttcaaaatt tttaccgcag
    32221 agggaatgtt tcccttttca cctttctggg ggaaaagaat tagaaagaaa gccagggcta
    32281 tcaccaaaac caaaattaaa actactaacc aaaagccact tcctccaaaa ggtagccatc
    32341 tttttcccaa atttatccag gcttctcgta actgtctcaa gcgaaaaaaa ttagaaaaat
    32401 aaggacggtt atcccgagga gggcgaaggt caaaagtaga ttctatgtct tctccctgga
    32461 gaataccttc cactactcga taatatcttt tttgagtatt aaaaactacc tcggcttctt
    32521 cttcctttat atctggataa tatataaaat caaagcattc cttttttact ttttctttca
    32581 tctttttcag ctctttttcc tcccacggcg atttcttaat catcaacaaa gcacaatcta
    32641 aattctttat tatcatcaga tgtttttcca ccctttctcc ttcctcccag gattctttta
    32701 caagattcac cagcttagga agcaccgcag ggggattctg aagaaaaaga gaaaacactg
    32761 ctaccccttc ccgagataga gaagaaaaaa cgctttgtat cccctcttta gtaaataaaa
    32821 aatcttcctg gaaagagaag ctaccgggaa aaacggctgc ctgagctacg ggaacttcca
    32881 agaaaattaa atcgtacttc cttccttgag ctaaaaactt acggggaaaa ataacctgcg
    32941 gttctgaata aaaaagggga taatccttta gaaaagaagc aaaaagagga gaagaaatta
    33001 cccaatccaa tttttccaat tctaaaaaag aggccaaata tccctctaaa ccctttctac
    33061 cttccacaat gagaatttcc cggggagaaa gtacatctac aggcaaataa tacaaagtgt
    33121 gagttaagag ggaaaaatcg gggtaaacag gaaaaagctg ggattgatgt ttatcgtaaa
    33181 ctaaagtcca ggaaggggga ggaaggtcag gaaaattaga actcaagccc aatgccaatc
    33241 gagaataagg agtataaaat acttccaaca attctgctgg actatggtaa acttcaataa
    33301 gctggttttc ctctacttcc cttattgccc gggcaggagc ataaggagaa agataaatct
    33361 caagaggaga aaaaaagaaa gtaattccaa taatcattaa taaaaaaaga gggacttttt
    33421 tcactctggt tgcactcacg agagaaagaa gggcaaggaa aagaagagct tttagctctc
    33481 ctagaaaaag aagaattaga tagcttatcc ctatccctaa ggaggagccc accagattgg
    33541 cagcataaaa gcgaggaaag tgtgagggat aaagttcaaa agcatacaac tgatacgagc
    33601 catgagcgaa gaaaggcaga aaagccaaaa agagaagagt agcaaaataa aaccagttat
    33661 aacgattgac ccagaattga taaatatcta aaggcaagaa gactaaccct gcggcaaaga
    33721 gcaaaagcga gagcgcaaga aaatagggga tgtgctcttt gaaccaccgg ggaagaaaag
    33781 gataaaggct tcccgctgct ccatagccca aaagagctaa agaaataatt aaggatacaa
    33841 aatgatactc aaaaatgaaa ctaaatatcc tcatagtgag agtttccacc gctaaagcta
    33901 ccgcagctac tcccagaaga gaaagagaaa atcttgtttc ctcccgcact gtaaccacca
    33961 ctgtatattt taactcttaa aatggccact tgataggtct gctaaaatat aacctgtatt
    34021 aatttagcgg gaggaaaatg atgaaagaag tagtcataag gtttaaaaaa ctctcagaga
    34081 atgcttttct tcctcgtttt tcttttcctg atgatgcctg ttttgacctc ttcagcccta
    34141 ttgataccat aatcgttcct caagaaagca aaatcataga tttagaaatc gcttctgaat
    34201 tccctcgagg atatgaagta gtgctgcgac ctcgaagcgg aatggggatt aaccatggta
    34261 taatagtgca tctgggtacc attgattgtg gataccgagg tagctggatg gtcagaattt
    34321 ttaatttagg taaagaaaat tacagtataa aaagaggaga caggattatc cagggagccc
    34381 tgcgagaaat tcctcaagtt aaaattatag aaactaattc attatctcct agcgaaagag
    34441 gaaaacaagg tctgggctcc accggtcgct aagtttcaaa agggaggaat agatagccat
    34501 atgaatatgc atatatttga taaactcaaa aatgagccaa ttatagctta tttatctatg
    34561 gaaatagctc tttcttcaga tatgcctacc tacagcgggg ggctgggagt tctggctgga
    34621 gatacagttc gttctgcagc cgacctctct cttccttttg tagctgtaac tctaataagt
    34681 cgcaaaggct atctgaatca aaaaataagc cctgagggat ggcaaatcga acaacctgta
    34741 gaatgggaaa tcgagaaaat cctgaccccc cttcctgttc aggtagaagt agaaatcgaa
    34801 aaaaggaaag taaaagtggg agcctgggtt tatccctgga aaagcgccag aggaacaata
    34861 gtgccagtaa tttttttaga caccaatatt cctcaaaata cccctgaaga ccgttccata
    34921 accgattatc tctatggagg agatagtcgt tatcgtctga aacaggaaat tgttttaggt
    34981 ataggaggaa gtagaatatt aaaagcctta gatttagaag tgagaaaata ccacatcaac
    35041 gaaggtcatg ctgctcttgt aaccgtggag ctattgcaaa gttttaaaaa ttgcgatgta
    35101 gaagcggtta agaacttatg tgttttcacc acccacactc cagtagaggc gggacatgat
    35161 aaatttcctt atgatatggt aaaagacgtg ttaggtgaac ctatccccct tcctcttctt
    35221 aaacagttag gaggggcaga ctgtctaaat atgactcttt tagccttaaa tctcagcgga
    35281 tatattaatg gagtagccaa aaaacataga gaggtttctc tcaatatgtt tccgggctac
    35341 gacatccatt ctattactaa cggagtacac agttatacct ggacctcccc cggcttccag
    35401 actctttttg atcactacat tcccgggtgg gctcaagaac cagaacttct gacccgagct
    35461 gatatcatat ctaaccagga aatctggact gttcaccaaa aagaaaaagc ccggcttctt
    35521 caattaatca aggaaaaaaa taatatagaa atggatgaag aagctcttac tttaggtttc
    35581 gctcgccgta tgactgccta taaaagacct cacttcatct ttaccgatgt agagagactg
    35641 aaaaaaataa accgtgcagg aaaattacaa attgtattcg ccggcaaagc tcatcctcat
    35701 gatttcgagg gtaaaaaact cattcagcaa ataattcaat attctcgaga attaagcccg
    35761 gagattaaaa tagctttctt agaaaattac aacttagaag tagctaaaaa aataatagct
    35821 ggagtagaca tctgggtaaa cacccccatg aggccgctgg aggcttcagg caccagcggt
    35881 atgaaagcag cccataacgg tgtgatcaac tttagcgtgc tcgacggatg gtggatagag
    35941 ggatgtcttg agggaataac cggatggtct attgggccag aaagcgaaga agacggaaat
    36001 cctcaggaga ttttccaaaa agagataagt gacttctacg gcaagttaga gtatgttatt
    36061 gttcctactt actatcatca ccgagaacaa tggattaagc tgatgaaaaa ctctatctct
    36121 aaagtcgcct cttactttaa tgcccaccgg atgatggaaa gctacataac cgaagcttat
    36181 tttcaccaac cttttctccc tctagcaaat ggttcaatag tcaaagcaga aaagaactaa
    36241 ttctactaaa tataaaatcc ggaggcactg ataacactga agtaaaaacc agcaatccct
    36301 aaaagcaaaa aagcctgggg caaaacccca ggcttttctc tttttcccta ctataatagc
    36361 ctctttactt tccctactat aatagcctct ttacttttac cgcttgaggt cctttatcac
    36421 cttgctgtac ctcaaactct acttcttggc cttgctctaa agttttaaag ccgctacctt
    36481 cgatagccga gtagtgaacg aaaacatccc caccttcgtc agattcaatg aatccataac
    36541 ctttggtggc actaaaccat ttaactttgc cagttgccat ttaaacttaa acctcccaaa
    36601 attgaattga aaattaataa gcttgcgcaa gctcggaaag catagtacca ccactccctg
    36661 gttttgtcaa taaccacatt ttttatttgt agttattctg tgataatttc tcgagagcca
    36721 agaggcattc cggccagctt tccaacttct ggaaaaaaag tattaacttg gatttaattc
    36781 tcttccagga agaaaccagg aaagtagacc acaattctac tttttctttc tctttggttg
    36841 ggtattggat tatgaatcaa ggtaaaactc cagagcagct cctcgctcta aaacactctc
    36901 tccacggcct tcgatcgtgt agtaaaggtc caacataatg gcactatcca tgaactaata
    36961 ctcgggaaaa gatgtcatcg aaaaggaggt ttttaaacct aataaatctc ctaaaatagc
    37021 gactctaaag aaaacacctc cgattctgtt catccttttt atttatttga caatgcttaa
    37081 atattttatt aattacaaac tatcctaaaa cccacgccca tagcggattc tacatagcag
    37141 aagatggatt ccgcttgtac cagttaacat ccagctcctt aactgctttt acctcgtcca
    37201 gacgccttag tggagtatgg tggggagctt ctttcagaat ttccgggttt tccatagctt
    37261 ctaaagcaat ttccctcatc gcttctccaa acctgtccag agtttctttg ctctctgtct
    37321 cagttggctc aaccatcagg gcttcttcga caacaagcgg gaaataaatt gtaggagggt
    37381 gaaagccata atccagcaat cttttggcta tatctaaagc cctcactccc tttgcttttt
    37441 gttttttggc tgacagcaca aactcgtgct tgcaagagcg gtcgaaggga agatggtagt
    37501 ctttttgcag ctggcgcatg aggtaattag cgttaaggac agctatctcg ctcacctcac
    37561 gcaatccttg cgccccaaga gaccttatgt aagcataggc tttgaccata accagaaaat
    37621 taccataaaa agattttact tttccgatgc tttttggccg atcatagtcc agataataac
    37681 tgttatcttt catactcaca agaggaacag gcaagaaatc ttccagctcc tcggtaaccg
    37741 caatagggcc agaacccggt cctccacctc cgtggggagc agcgaaggtt ttgtgcaagt
    37801 tatagtggac gatatcaaag cccatgtctc cagggcggga tatccccaga ttggcattca
    37861 tattagcacc atcataatat aatagacccc ctgcctggtg gacaatgtgg gcaatttcta
    37921 cgatgtcttc ttcaaaaagc cccagtgtat tggggttggt caacattaaa cctgctgtct
    37981 ggggtccggt gatttttcgc agattcaaaa catctactcc tcctcggggg ttagatttta
    38041 cttctaccac tcggtagcca cacatagccg cgcttgctgg attagtcccg tgagcagagt
    38101 cgggaacgat gatttcctgt cttttgtggt tttcacctct atcacggtgg aaagctctta
    38161 taatcatcaa tcctgtgagc tctccgtgag ctccagctgc cggctgcata gtagcccgat
    38221 acagacccgc tatttcacac agatattttt ctaattcata catgagcttg agtgctccct
    38281 gcacagtttc ttctccctgg taggggtgaa tcctttgaaa gccagcaaat ttagctacat
    38341 cttcgttaat tttgggattg tacttcatgg tacaggaccc caggggatag aaccccacat
    38401 ccactccata gttgagtcgt gatagttggg taaaatgcct gactacttcg ctttcgctca
    38461 gctcaggaag atcagcggct ttttttcgca aaaatcggga gggaatgagg tcagtcacct
    38521 tttctgaagg gacatcacaa tcaggatatt cataacctcg acgccccgga gagctcattt
    38581 caaatatcaa aggtacttca cttgggttat tcattggcca cctcctccag tacttgcgtc
    38641 agggcatcaa tatcttccct ggttcgggcc tcagttacgc taaaaagcat ggccggaccc
    38701 agttctgggt aatatgcttt taagtccagc ccccctatta ttccccgctc ccatagtgct
    38761 ttatttattt tctccggtgg aagaggagtg ctgactggaa attcgctaaa aaatgctccg
    38821 ggaaatagag gagcgaatcc cttaatgctt acaatttttc gataagcata gtgggctttt
    38881 tgcaggcaca agttggccac ctcctgtagc ccttcttttc ctatcaagct gagaaaaact
    38941 gtagcggcaa gagcattgag agcctggttg gaacatatat tggaggtagc tctttccctg
    39001 cggatgtgtt gttctctggt ttgaagagtg agaacaaaac ctctccttcc ctgggcatca
    39061 tttgttgctc ctgcgatacg tccgggcatc tgtcgaataa atttatcgcg ggcggcaaag
    39121 aaccccaaaa acggacctcc aaaagccacc ggacctccca aactctgccc ttcccccact
    39181 gctatatcgg cgccataatt gcccggtgct tccagaattc ccagagatat aggattaacg
    39241 cagacaataa acaaagctcc attttcatgg gctaaagagg ctatttcttc tacttcctct
    39301 aaaagccccc agaaattggg ttgctgaatt aatacacaag ccacgtcttc gtctaagttg
    39361 ttttttaagc tatggaggtc aacctggccc ctatcatagg ggacatattg cgcttcaaga
    39421 ccccggcttt caaggtaaag ttgagtgact tgccgatatt caggatgaat agttctcgct
    39481 atcagtgctt tgcctcgtcc ggtagcccca caagctaaaa tagccgcttc ggctaaagct
    39541 gtcgccccat cgtacataga agcattggct acatccatct ccgttaagtt gcaaattaag
    39601 ctttgatatt catacatagc ttgcaaagtc ccctggctga tttcgggctg ataaggggta
    39661 taggaggtat aaaactcagc gcggtctaag atgtgctgta ctgcactggg aattaggtgg
    39721 tcatatgcgc cagctcctaa aaaagaagga tatcgatcca ggtcggcatt tttttgagcc
    39781 agagctctca gggagtttag cacttctatt tctgattgag ggggtggtaa gtttagttcc
    39841 cctcgaaaac ggacttcttc aggaatatca ttaaataact cctcaataga ggatattccc
    39901 aagcttttta gcatggcttc ccgatctttc tctgttgtgg gtatgtatct caaatctgtt
    39961 ttcaccacct taaattttaa tgtagattac cagggttatg gttttagttc gttctcaatt
    40021 aaagattgat aatcagaaac tttcatcaag gtgtggagtt cttcttcccc ggcgatctca
    40081 atgacaaata tccacccctc tccgtagggg tcttggttga ctaactccgg ctggttttcc
    40141 aattgtttat taacttctat tattttcccc gataccggtg catacacatc tgctaccgct
    40201 tttacagatt ctaaagtggc aacagcatct ccggcctcaa cttcctttcc ttcctcggga
    40261 agctctacaa aaaccacctc gacgagctgg tcctgagcat aagatgtgac acctactatg
    40321 gctcgatcgt cttccacttt gacccactcg tgacttttgg tgtaaaggag atcttcagga
    40381 tacacggttg gtcctccttt cgattatttt tttattcggg gtggaacaaa aggacccgca
    40441 gttatttcag ctctaacttt tttttctcga atcaacagct caatcactgt tcccggtttt
    40501 tccagagcag gtggaacata acccatgcca atgtttttct tcaagctggg ggagagagtt
    40561 ccgctggtaa ccacccctac ttttttttcc tgataaaaaa tgggataacc ctggcggggg
    40621 atgccccggt ctaaagccac aaagccaacc aggcgtttct tgacccctga ggctttttgc
    40681 tcaaaaagag catctcttcc gatgaaggaa tgattgaatt tcaccaccca ttccaggttt
    40741 gcttcgagag gagtggtggt ttcatcaata tcattaccgt acagaggata tttcatctcc
    40801 aatctcaagg tatccctggc tcccagtcct actggagcta aagatagccc cgctatcttt
    40861 gccgcctcac cgagcaactc ccaggttttt ggcgcttcgt cccaggggat gtatatttca
    40921 aaaccatctt ctcctgtgta tccggtgcgg gaaaggatgg ctctgaagtt ctggattttg
    40981 gtttctaaaa agttatagta ttttaattct gaaacagcgg gttctatatt taccataatt
    41041 ttttctgaat cgggcccctg tatggctatt aaagccaggg aatcggtcag attttctact
    41101 ttcactggtt ctcgaagatg agatttaatc cactcatagt ctttggcggt gttagctgca
    41161 tttactacca gaagaaaccc gtcttctaac ctgtagacca gtatatcatc tataatcccc
    41221 ccgctgggaa ggcaaagaag agaatactgg attttgcctg gtgatagttt ggtagggtca
    41281 ttggtggtta gtttagcaag ctccaactca caacctttcc cataaatttt caactctccc
    41341 atgtgggtta aatcaaatat tccggcttga gtgcgaacct tctggtgctc gctggaaatt
    41401 gtttcataca aaaggggcat ctcccagcca gcaaagggaa tcatcttagc acccattttt
    41461 ttgtggatat ggtttaaggg ggtttttctc agttcttcca ttttaaatca cagcagaaaa
    41521 aacgctattt ttcaacacct ccttcagagt tttcaatgtt ttataaaaaa actaaagaga
    41581 gtcagattga cgctggttta gttgcaaata atgtggttct cccctctctt ctattccagg
    41641 taacattgtt attgtctttg tggtgtcttc ttgattaatt tattagtcaa gccagattac
    41701 ctaattcgta acacgccttt aaaaaattgt caatagcttt ttctgagtat ggctattcta
    41761 agatgatgtt aaacagtcta cggcataaga aaagagataa aaacataaac taaaaaatct
    41821 gccagttaaa aaagacatct tctacagcta actataaaat cttcttgaga aaatgtcatt
    41881 cggtcaggaa aaacagatta tgtctgaaaa attagaaaat ctaaaaatca tcttctcttt
    41941 ttagagaaaa taggttaagc ttaaagtaaa ttacgaaagg aggaaaaaaa tatggaaatt
    42001 tctcatattc tggtacccac tgatttttct cccttttccg atttagctct ggaacacgct
    42061 gcttccttag ccgaaaaatt cggggcaaag ataaccattc ttcacgtttt aactctggct
    42121 gaagttaatg ccctcacctc tcagcccggt aacccctggg aaaacattgt gcttaagatt
    42181 cagaacaata tgagagaaga atttgaaaaa aatgccggaa actactctat agttgaggag
    42241 ccagtattgg aagtagcagt aggagaacca gcagaagaaa tagccaaatt tgctgaagaa
    42301 aatggagtaa accttatagt tatgggaact catggaagga ctggtttggc cagtatattt
    42361 ttaggtagcg tcaccatcgg agtaataaag aaaacctgcc tgccaattat ggtagtaaaa
    42421 tgtgctgaga gtttgaaata accaaaatag aggggttttg atatactcaa aacccctcta
    42481 tttgaaacta aaaagatagg acatatacct gagatatcag gaaatttgta aaacttagta
    42541 agctcccgct atggaattat atattcaact gactgagaaa aaggaattag ttttctcact
    42601 tattcagcgt agaaaactca ggtaaataat ctcaaccccc gggaaaataa gtcatcaaaa
    42661 tttactcccc ggtaaatgag gtcttcttct tcgatttgag agtatatctt atcctgaaaa
    42721 gtgggacgct ggtgctgata tatcactccc aaaggaattt tatctcccca ctccaggctt
    42781 ttttccagag ctttaattct atcttccggc tgatagctat catctaagta ataaactctc
    42841 tctttatacc aggcaaaagt attgacccga ttaaaagtta cgcaaggttg caaaacatct
    42901 attaaagaaa agcccctcca ggtgagagct tcttttataa tttctttcag tttatctaca
    42961 tctcctgaaa atcctcgagc tacgaagctg gcatcttgag aaatagccaa actcaaagga
    43021 tttaaaggaa gagataaatt gccccagggc tgagctttag aaacaaatcc ttcttctgaa
    43081 gtaggagaag cctgtccctt ggtcaggcca taaacctggt tattatgtac aattaccgta
    43141 atatcagggt ttcgccgcag agcatggaga aagtgatttc ctccttctcc atacatacat
    43201 ccgtctcctg attctgctat cacgagaagc cgggggttta cggttttcac cgctaaagcc
    43261 acaggaatgc ttcttccatg caatccatta aacacatttg ccgctatata atgaggcagt
    43321 ttagctgctt gaccgattcc agaaaccaga actacctctt ccggggaaaa tcccaactcc
    43381 gtgagtgctc cctttaaagc tcttaaaatt ccaaaatttc cacagcctgg acaccaggcg
    43441 atttctacct gactatcaaa gagattaccc atttttcccc acctccttga ccttaagcag
    43501 taattcttcc accaggaaag gccttccgtt atagctatta attctaaaag gaaagggcaa
    43561 tttaagttct ctttctaaaa gattagccag ctgccctcca taattatttt ctaccactac
    43621 aggaagagaa acatgactga aaagttcctc tatttgtaga ggaagaggat aaatttcgct
    43681 gaagtgaatt attccgctat ctatcccctc tgctttaagt ctttcgcctg cctcctctat
    43741 tactccccag gtagagcccc aaccaattaa agttaaatct ttcccttctc tatgataggg
    43801 taatcgaact tcttttctca ttgtctcttt cttcctcatc ctctttttgt gcatttccac
    43861 tctcaaattt aaatcttcgg tgagatgtcc aaactcatca tgttcatcgc tatctgctac
    43921 taccagcgct ttacccaacc ccgggatagc ccggggagag acacccgagg gagatatgag
    43981 gtagcgccgg taaggaagag gaaaagaact aaattgggga gcatccggcc tttctttcac
    44041 ttccaaattc tcataagtcc atttagaatc agctaaatat tgatcgctta agataatgac
    44101 tggcacttga tacttttctg ccagattaaa agctcgaatc acgagattga tagcttcctg
    44161 ggggtcacga ggagcaaaaa caaaacgagg gaattcatca tgggaagcgt ggattacaaa
    44221 ctctaaatct ccttgcgagg tccgggtggg taaaccagtg ctgggaccgg gtctttgagc
    44281 atccaccacc accaagggtg tttctgtcat agccgctaag cctaaaccct cggccataag
    44341 acaaaagcca cctcctgagg ttgccgtcat agcccgagct ccagcatagg aggctccaat
    44401 agccatatta atggctgcaa tttcatcttc tgcttgttct actaccactc catactcttt
    44461 ggcatgagaa gaaataaagt tcaaaattcc cgtagaagga gtcataggat aggcgctata
    44521 aaattgacaa ccacttaata aagctcccca acccaaagct tgattgccat ccagaaccaa
    44581 aagagaagaa ttccctctcc tggtttcctc tatcagtgga gaagaaacat agggaattac
    44641 ttcgtctcta ccccgcctca gggcttgttt gtttttctct actatttctt ctcctttacg
    44701 ggcaaaaatc tcttctaagt aactatcagc gctctctaaa tctaatccca gaatacccaa
    44761 tatagcaccc acagcaacga tgttagcaaa aatcttattt cccacaccct cagcaatctt
    44821 ttcaaagttt agaggcagga aatgttgaaa atgagcttct ctttcatttc ctaccgcacc
    44881 taaaacccat cctccctctt ttactcttct ccgatgcaat tggtaggttt cttcattaag
    44941 acaggccaga atatccactt ctttcaatgc cgcccggagg ggattagtag aaaagcgaac
    45001 ttgagtaaag ctatgccctc ctcttatccg ggattgataa tcctcaatgg aaaaaacaaa
    45061 atatccactc ctttgtaaaa tagtggatag agctccagaa acagtttgca ctccctgccc
    45121 cgcttctcct cctactaata agtttaagtc caaattttac ccacctcctt tagaaaacta
    45181 atacaactca ggcatacccc cctgctatac acaaagggta tcaatgcaag aaggagcact
    45241 gcccaaatgg tatcacgagt aaatttttga agttgtttct gttacttaat ctctctgcgc
    45301 catcctatgt tttattgcta aaccagatca gtttttaagc attgtgattg cactcgatat
    45361 attaccacat caccggggaa attcgagata actgataaga gagaaatcga gtaaagagcg
    45421 actcacttct atcctaccgg atagtcactc aggaattagt ctgggttaga agatataaat
    45481 taaaaactta acaatttctg tctaatttct attgaagcat tacaatcttt atgccttctt
    45541 gatcaaaagc tttttttata agataaagta tatctctttc tacatcccaa tgttcattgg
    45601 gagctacttt gagcactaat ctcaaaacca ctctggaaga atctaaacgc actatccgat
    45661 gaatagtagc tggctctaaa atcctccggg gatgttctct atgatatttt tctgctacct
    45721 cttctaaaac acttatagcc tggtctatat ctgcttcata tgctatcccc acctctacaa
    45781 tagcccggct aaagcctcga ttgtaattgg caatgcggga aatctctcca ttgggaacaa
    45841 caataagagc tccagaaaaa ggacgaatac gggtcactcg catccctata tcttctactg
    45901 tgccggtaat atcaccgatt tgcactgtat ctcctactga aatactatct tccagaagca
    45961 agaaaaatcc ggtcaagaca tctttgacca gagtttgagc accaaaacct actgcaatac
    46021 ctacgattcc cgctccggct ataatggctg tagcatcaac tcctaactga cgaagaatta
    46081 aaatagcagc gaaaaaaata actacgtatt ttaatatact ccgcaaaaga ggtaccaggg
    46141 tgttaactcg acgagctaat tctcgccgat cggtatattt ttttcctccc caggacagaa
    46201 ttttccttat tagcctctca cttatcgata aaataaagta agaaacaccc acaataatag
    46261 caatgtttaa agctcttatg agtacagttt ctaaaatact gacactggtt tccggcatca
    46321 atctctcctc aaagccagag taataccctg tctttcaaaa atctcctggt gatattcctg
    46381 gatggattta acagttatag agccagactt gagacgctct atagcataaa ccgtggcttc
    46441 tgctcctgag agggtggtaa caaaaggaat gctgcgaata atcgcctctt tacgaattat
    46501 gctactttct aattgagcac gccctccaga aggagtatta ataatcagat ctatttgccg
    46561 gttcttaatc acatccacca cgttagggcg tccttcccct accttttcta ctatttctac
    46621 tggaatacca ttattactca aaactttagc tgttccacgg gtagctacaa ttttaaaacc
    46681 taaatcacga agctttttag ctataaaaat tatccctctt ttatcccggt ttttaacact
    46741 aatgaatacc gtaccttctg agggcagata aaattgagca gcaatttgag acttggcaaa
    46801 ggccatccca aaatcgctat caattcccat tacttctccc gtggagcgca tctccggacc
    46861 taataccggg tctaccccgg aaaaacgccg gaaagggaaa actgcttctt taaccgcaaa
    46921 ataatctatt tttacttctt cagttaagcc tatttctcgc agctttcttc ctgccatcac
    46981 ttgagaagcc aggcgagcgt aaggaacgcc ggtagcttta cttacaaagg gaatggtgcg
    47041 agaagcacgg gggttcactt ctaagacgaa aacctcgtcg tctctagctg catattggac
    47101 gttcataagg cctaccactt taagttctcg agctaaagta taggtaatct gcttcacttt
    47161 ttcaatgatt tcatcgctca gagaaaaggg aggtataaca caagcactat ctcctgagtg
    47221 gatacccgct tcttcaatat gttccattat accggcaata atggtggtct ccccatcact
    47281 gatggcatca acatctagct ctattgcatc ttctaaaaac ttatcaatta aaatgggatg
    47341 tccggcagaa acttctactg cttgcgagat gaactctttt agctcctctt cgctataaat
    47401 tattctcatc gctctccctc ccagaacata ggaaggacgc accataaccg ggtagccaat
    47461 ctgagcagcg accaaatgag cttcttcaaa agaggtagct attccactgc ggggctgttt
    47521 tagtccaatt ttatcaacta attccttaaa ccgatctcgg tcttcagcca tatcgatact
    47581 ttctggagaa gtccctaaaa tttttactcc tgctctctct aaacctttag ctaaattgag
    47641 aggagtttgt cctcccagct ggagtatcac tccgtgaggg ttttctttct ctaccacgtt
    47701 aagaacatct tctaagtaca ctggttcaaa aaacaacttg tctgaggtat cataatcggt
    47761 actcactgtc tccgggttgc tattcaccat gatagtttca taacccaggt cccttaagcc
    47821 ccaggtcgca tggacacaac aataatcaaa ttcaattccc tgccctatcc gattgggtcc
    47881 tgcccctaaa atgattgcct tttctctctt tgaggggtta gcttcacatt cttcctcata
    47941 agtagaatag taataaggag tttcggcagg gaattcaccg gcacaggtat ctacttgttt
    48001 gtaaaccgcc tctattccct tttcttctcg ccaccttctc acttcttctt cttcggtatt
    48061 ccagaattca gccagctggt gatcagaaaa accaaattct ttggctcttt tccctgattc
    48121 cgggttcaaa tttttccagt cttttatatg cttgagctcc tcttccatct ctactatttc
    48181 tactatctca tgaataaacc agggatcgat tttagttaag ttataaattt cttctatgtt
    48241 taaaccctct ttaatagcca tttttatata aaatactctt tcagcattgg gtttagccaa
    48301 cctttcccgt aatatttctt gtcgcttccc cggagaaaaa tcctcccagt ttttgatatc
    48361 ctccagacca tacttaccga tctccaggga acgaagtcct ttttgaagag cttctttaaa
    48421 attacgccca atagccatag tctctcccac actcttcatg gaaattccta accagggttc
    48481 agtttcagga aatttttcga aggtaaaacg agggattttc accacacaat agtccagagc
    48541 tggttcaaaa gaagctggag ttttgcgagt aatatcattg ggaatttcat ctaaagtata
    48601 acctatagct aatttggcgg caattttagc aataggaaag cccgtagcct tggaagcaag
    48661 agccgaagaa cgagaaaccc gggggttaat ttcaataatt acaatccgac cattttgagg
    48721 attaacggca aattgaatat tacagcctcc tgtatcaact ccaatttctc ttatagcttt
    48781 gatggctaaa tctcgcatca actcatattc ctggtcagtt aaggtttgag caggagccac
    48841 ggttatgcta tcaccagtgt gtattcccat agggtcgaaa ttttcaatag aacaaatgac
    48901 caccacatta tctttagcat ctctcataac ctccagctca aactctttcc agcctgctac
    48961 tgattcttca atcaatattt ctcgaatcaa gctactatcc aatgcctgtt gagctaaatc
    49021 ttcaaactcc tcaatattgt aggcaatagc tcctcctgtc cctcccagag tgaagctggg
    49081 tcttataacg agaggaaaac ctatctcttt agctattcgg attgcttcct ctaaactata
    49141 agcttgtccg cttcgaggga cttctattcc cactttagtc atggattctt taaaaagtcg
    49201 gcggtcctct gcttttttaa tagcttgaat agaggcaccc aagacttcta ctccccactt
    49261 atttagcact cctcgctctt cgagctgtac agttaaattt aaggcagttt gtcctcccaa
    49321 agtgggaagc aaagcatcgg gttgctctcg ctctataatc ttttctaaaa tttcaggaac
    49381 taaaggctcg atataggtac gatcagccat ctccggatca gtcataatgg tggccgggtt
    49441 actgtttacc aaaaccactt tgtaaccttc actctttagc gctttgcacc cctgaactcc
    49501 tgaataatca aattcacaag cctgcccgat tactatagga ccggaaccaa taatcaaaat
    49561 tttctggata tcttcccttt tagccaagat tatcctccac catctttata aactcatcaa
    49621 aaagataaac agtatcatga gggccgggat gagcctcagg atgaaactgc accgagaaaa
    49681 agggtaaacg cttatgtctc attccctcca gagtaaaatc gttgagatta atgtgggtaa
    49741 cttctacctc gggaggtaaa gtatcggagt caacaacaaa accgtgattt tgagtagtta
    49801 tggccaccca gccagagcgt aaatctttaa ccggatgatt agttccgtga tggccaaatt
    49861 tcaatctgga agttttccct cccacggctt gtgccattat ctggtggcca atacatattc
    49921 ccataatagg aaacctctcc attatctttt gcaccagatc taccagataa gaaagggaag
    49981 caggatctcc tggcccatta gaaataacca ctccgtgagg tttttccttt ataatttctt
    50041 ctaccgggga agtagcggga taaactacta ctcgacaatt tctcttggcc agttctttta
    50101 atatgctaaa ttttaccccg caatccacaa ccaaaatgtt aaattttccc tcttcctgcc
    50161 acacataagg agcaggagtc gtaacttccc tgaccaaatc ccgaccaatc atggggggaa
    50221 tttgccgcgc tttttttatc aaagaggccg ggtctgagtc tcgggtggag attactcccc
    50281 gcaaagcccc cttctcccgg atgtgcctgg taagggctcg agtatctatt ctttctaatc
    50341 ccagcaagtt atggcgtcta aaatattctt ctagggtttc ctctcctctc caactactat
    50401 aaatggtact tttttctcta acaattaaag cttctacttg aggacgagaa gattctgaat
    50461 cttgagagtt gatgccgcaa ttaccgacga gaggataggt tatggttacg atttgacctc
    50521 gaaaagaagg atcggtgagg acttcctggt agccggtcat gccggtatca aagattactt
    50581 ccccccctgc ttctccttcc gtaccaaaag cataaccctg gaaaattctt ccatcttcca
    50641 aagctaataa cgctttcatt ccaacaccct cggttaaata taacataact gtcaagaggt
    50701 agaacttcag attttaaaac cgggaaaagg atgaaattct ccttttatgg tatcctacaa
    50761 aacctgttat taccaataag agggatactc tcttctttca tcactgcatg accgcaaggg
    50821 aatatcttac tatatagccc ttttgagaaa taagcctctc aacgacgaca gaaacattgt
    50881 aatgattttt gcagttaact ctctccgggg gcgacatagc gatttgctaa ccaattttac
    50941 aaaatgtaga aatgcaaaat ttcttaactt aagagttcaa aatctattca gcttcctcgc
    51001 tttttcaaaa aaccattagt ttagttacac ttatatcttt ccttttccgg aatctgggag
    51061 agctaatcta taaatgttca ttgacagata agggtataaa attatagaat agtagtggat
    51121 tagattaaac cctttggttg cgcgcctgta gctcaatagg atagagcggc ggacttcgga
    51181 tccgtaggtt gggggttcaa ctccctccag gcgcgccaag gcttcacaga accattttcg
    51241 ccttctctac cctctctaaa aacccctgag tccgacgact ataaatctac tcccagaaag
    51301 aggcgatttc tgtggggcag gtagtaaaac t
//
LOCUS       DAOPSS010000002        44067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_643_length_44067_cov_9.348428, whole genome shotgun sequence.
ACCESSION   DAOPSS010000002 DAOPSS010000000
VERSION     DAOPSS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 44067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44067
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_44067_cov_9.348428"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKN31_00220"
     CDS             complement(<1..679)
                     /locus_tag="PKN31_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA98381.1"
                     /translation="MSYLDSLKAFTTEQVVKLMVNSLRNASDENIIRLTYLAEKLTPI
                     EYYRDQIRALRQMFEEQRPQIVLARRVLQETHPNVREKLVVNLIVKSILLGVPKRQKL
                     EKELGVQVPFFFVVSPTMRCNLNCYGCYAGQYRKESDMPIELLDRLFNEAEEMGMNFL
                     TISGGEPFIRKEHLDLFEKHPDIAFQIYTNGTLINQEVAKRLAQMGNVYPAISVEGYE
                     KETDERRG"
     gene            complement(727..1332)
                     /locus_tag="PKN31_00225"
     CDS             complement(727..1332)
                     /locus_tag="PKN31_00225"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOA98382.1"
                     /translation="MIKSSISKEELLEEIKSEVKTCTLCSLSQSRTKIVFGEGDPDAL
                     LMFVGEAPGEEEDRTGRPFVGKAGQLLTKILQSVGLKREEVYITNIIKCRPPGNRTPA
                     QSEMDSCFPYLEAQIAIINPSIIITLGGTSTLYLLGKKEPISKLRGQWFDWRGGKKIF
                     PMFHPSFLLRYNSRQPGSPRYLTWKDIQEVKRTYDLLKQSS"
     gene            complement(2330..3241)
                     /locus_tag="PKN31_00230"
     CDS             complement(2330..3241)
                     /locus_tag="PKN31_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOA98383.1"
                     /translation="MREQKFSSEEIPSNPLASKAYYARLEAWISILVNFTLFGIKLYY
                     GRLSQSLALTADAFETLSDVATSLAIFFIARELDRPPDREHPFGHGRLEDTTTLIIAV
                     LLFIVGIQFLIQGCQRIFHPSPIQFSLPALFAILIGMGGKEILAQVAFFLGKKASNSE
                     LLIAKAWHHRSDALDSLPVLVVFLFPRYPWLDGVSSLFTSTIIILMASKILRGSVSRL
                     LGRSISPEEKEKILNITSQFKEVDNVHSIHLHDYGGKGVITMHIEVDGSLSVKEGHDL
                     SEKIEKEIKEQTGFEGIVHIEPLSEEK"
     gene            3469..3915
                     /locus_tag="PKN31_00235"
     CDS             3469..3915
                     /locus_tag="PKN31_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98384.1"
                     /translation="MARKMGKKLQTEVSSESGMIDIFILVVVSFLLIISSWFLGNVLL
                     HYQVVDAHIEKFNEEYKIEGALVEAIQLLKDKGSTFQGDNIPSSYSSLFYFSVEEGKL
                     IISKKIEGQEKEEENMLPVAEVTFTWEENELKINRIETRYNLPYSK"
     gene            3937..4506
                     /locus_tag="PKN31_00240"
     CDS             3937..4506
                     /locus_tag="PKN31_00240"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOA98385.1"
                     /translation="MENTNKDKTKEINQRGRGNIALLGFMGSGKTTIGQQLAQKVGWQ
                     FMDTDDLIEEKTSLSISLIFERWGEEHFRSLEKVVLEEVASKKNIILATGGGLPAQED
                     NWQILREHFLTIFLQVDFSILYQRIKGDSRRPLLSQYSTPQELEKLYQKRLIYYERSD
                     VIINGNRSEEEIVEEILYHEKISSLWRKR"
     gene            4472..5560
                     /gene="aroB"
                     /locus_tag="PKN31_00245"
     CDS             4472..5560
                     /gene="aroB"
                     /locus_tag="PKN31_00245"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOA98386.1"
                     /translation="MRKLAVCGESGKQYPVFLGWDLLERDDWLEEIKFPSSFGVIVTN
                     SRVGSLYEERVSSFFSRQGFTIQVVTIPDGEEYKNLSTVEFIYKQLLNLDLDRKSFLL
                     ALGGGVITDITGFVASTFLRGIPYFSIPTTVLAQVDSSIGGKTGVNLPQGKNLVGTFY
                     PPAGVFIDLSFLRTLPPREYKEGLAEVAKTFFLAGGEILSLLEKREQILSREEEILEE
                     LIFQSLSLKKKIVEEDEKEKGIRSILNYGHTIGHALEKVEGYGKLRHGEAIALGMMGE
                     AILGEKLSITPSKVVDKQREILANFYLPTQLKFKIEREEFFAALRQDKKKERGKARLV
                     FLKDFGQPIWGVEVEEEKIGEALSYLER"
     gene            5568..6008
                     /gene="aroQ"
                     /locus_tag="PKN31_00250"
     CDS             5568..6008
                     /gene="aroQ"
                     /locus_tag="PKN31_00250"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOA98387.1"
                     /translation="MNILFICGPNLNLLGEREKEIYGEKSFEEINIILEKEAQKMGVN
                     LEIFQSNHEGNIIDKIQESRGSVDGIIINPGAYTHYSLAIRDALKVVKAPIIEVHLSN
                     IYSREEFRHHSVIAPVCWGQIAGLGYQGYILALQAFLMRGKNEP"
     gene            5998..7047
                     /locus_tag="PKN31_00255"
     CDS             5998..7047
                     /locus_tag="PKN31_00255"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOA98388.1"
                     /translation="MNHKKRMAQLKEDLEHPFLVTEREDLFYLLGFQGSTGFLLVFPD
                     KTPVFFCDGRYATQAKEEIKLEVEIQEFRDEITKNIKEAVSGNGYQKLLVEDSLKIRF
                     YELLKNQGLEVAPVSSPSRQMRMIKDEEEIEEIKKAVRISEEALKKVRSLFREGVKEK
                     DMALELDYQMRLLGGEETAFPTIVAGGKRSALPHARPSSYSFKDNDWAIVDWGARVNG
                     YCADLTRVIVIGEVDSRFYQLLRIVEKAQQLAETIIKEGVKAKEVDKVVRSFFKEEKV
                     EEYFNHSLGHGVGIEIHEDPRLGPKSEDILKKGMVVTVEPGIYFPDWGGIRLENLVVV
                     QEEGNKILDSLPLLL"
     gene            7061..7627
                     /gene="efp"
                     /locus_tag="PKN31_00260"
     CDS             7061..7627
                     /gene="efp"
                     /locus_tag="PKN31_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOA98389.1"
                     /translation="MPEYISSNDLRVGTCIEVDGNLYVVTSFQHIKPGKGGAIIKTKL
                     KDINTGFIIEKTFRAGEKIIRAILEVKKAQYMYEDAAGYHFLNLESYEEIVLPEEMVG
                     ENRDFLVENLEVEILTYQGKCIGVELPPFIELEVVETTPGIRGDTASGGSKPATLETG
                     VVVQVPLFVNEGDKVRVDTRTREYLERV"
     gene            7701..7988
                     /locus_tag="PKN31_00265"
     CDS             7701..7988
                     /locus_tag="PKN31_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOA98390.1"
                     /translation="MQALVDEVVEGFNGVESWEKNSEESLRIETKKGAISLQLFLILN
                     LKKRVPETAWELQKGLKEMLEKKTGLRVDRIDIYVQGFAVHQKNSTVLAME"
     gene            7990..8427
                     /gene="nusB"
                     /locus_tag="PKN31_00270"
     CDS             7990..8427
                     /gene="nusB"
                     /locus_tag="PKN31_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOA98391.1"
                     /translation="MLRRKARVAALQILFQMDMRDKKLEEVLPYFKVPKGWDEDTLSF
                     FFRLVQGVETKKKEIDEILSQASEEWPLYRMANVDRNILRLAVFEILFLPEVPVSVAI
                     NEAVELGKKYGTEESGRFINGILGQLVRSLEERGNYLPKRERT"
     gene            8424..9311
                     /locus_tag="PKN31_00275"
     CDS             8424..9311
                     /locus_tag="PKN31_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOA98392.1"
                     /translation="MKIEDFLQENAHLVDEYLERNFAPLSATSSLVFPILYGAKGGKK
                     IRASLTLATGFGYGVEKEHLLPVAGAIEMIHAFSLVHDDLPCMDNDDYRRGKPSMHRA
                     FGEAMAVLTGDALLIEGLRLIVGNEELAKLFGKEKVLDMTNVILKALGIEGMVGGQVL
                     DLEGEGREVSEEKVWEIYRKKTGSLLTASILCGAIAGSAPSQEKEILENFGMLLGECF
                     QIKDDILDVTQEKEVLGKTPGKDKIQDKATLVKVKGVEESEKIMKDKWEEAICLLSSI
                     PRPFPFLLEIARFVIERKY"
     gene            9314..10129
                     /locus_tag="PKN31_00280"
     CDS             9314..10129
                     /locus_tag="PKN31_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOA98393.1"
                     /translation="MKKGKIRLDELLVESGLCDSREKAQRIIGSGKVKVEGLSQHLKP
                     STLVEKGVSIQIEEPPRFVSRGGEKLEKAINEFGINPQDLVFADIGASTGGFTDCLIQ
                     RGASKVYAIDVGHGQLHEKLRHHPQVVVKEKVNARYLDLSFLGERVDGVTIDVSFISL
                     RLIFSPATSILKDNGILIALIKPQFEAGRREVKRGLIKDKKVHERVLEEILTQAQEAG
                     LSFQGLTFSPLRGAKGNIEFLGYWKKERSDLTNEGLRSIISRVVEEAHQSKRK"
     gene            10154..10432
                     /locus_tag="PKN31_00285"
     CDS             10154..10432
                     /locus_tag="PKN31_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98394.1"
                     /translation="MRDNIFLRFGSSGYYICFISLDKTPKKKNYLSRAQVAEREAASL
                     LENSGFKLVGEQPEREFFIRVNGKKIPCKIRADYLVRRGRRDIYMWLK"
     gene            10420..10593
                     /locus_tag="PKN31_00290"
     CDS             10420..10593
                     /locus_tag="PKN31_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98395.1"
                     /translation="MAEVKTGEYLRFDHSRVRKQLLECYLVYHPKAIILVNGESNELY
                     QVAFPFSSASTWR"
     gene            10697..11500
                     /locus_tag="PKN31_00295"
     CDS             10697..11500
                     /locus_tag="PKN31_00295"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOA98396.1"
                     /translation="MRDKARELDNFLQSRGINTLIVEGETHIEEKPEVVFVLGGDGTF
                     LSASWLYSPLEIPLLGIDMGGLGFLAEVSVENMFQAAEEVIKGKFEVEERMMAQCRIV
                     YGDGSSNTDFALNDVVIYRGPFAQMIRLSTYIDGEFLASFPADGLIVATPTGSSAYSL
                     SAGGPILHPILELLVVTPICAHTLYARSIVVAPSSTIEIVLESEKEGTMVTLDGQRGY
                     SIQKGEHVTVNKSPFSVRLIHVEPKRPFYSLLRSKLSWGMDIRKPVEEC"
     gene            11494..13164
                     /gene="recN"
                     /locus_tag="PKN31_00300"
     CDS             11494..13164
                     /gene="recN"
                     /locus_tag="PKN31_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOA98397.1"
                     /translation="MLKTLRIENFVIVDYQEIDFAPGFNVVTGETGAGKSLLINAFSF
                     LLGARPREEWIRRESDFARVEGWFDISFSPFLQERLREEGWNEEIKGEMVLSREVNRE
                     GRSKARINGRSVTLTTLKEVASHLVDIYGQGENNRFLTPENKLAILDSFLDEEGKLGR
                     ENYLHLREERNEIKKEIQKLKRGQEVELEEIKEMLEEMERMGLTPEKIEEVEEQFQRL
                     VNSQRYREVVEEVISLLSGSEEKEGFLSSLRRIRRSLEGKNELALFPPSVEETLKNVE
                     LELQEVLYQVEKVKDSFDLPPSKVEELEKEIAEIERWKRKYHCLTTKDLIHFIGEKKA
                     KLSQLHQELEELEEWERELEIKEKNLMEAREKLFRHREKAAKIFKEKLEKELKELSFP
                     KVALEIELEKKEEDDEVSFLISLNPGEPLFPVERVASGGELSRIILAIKSLTREVREI
                     PILVFDEIDQGVGGQTAFWVGDKLRKIAIGHQVISITHLPQIACFAHHHLKVEKRGDE
                     ESTWVEVFSLSAEERVKELARMMGDEGRDDSALQYAETLMKRAQNANT"
     gene            13151..13855
                     /locus_tag="PKN31_00305"
     CDS             13151..13855
                     /locus_tag="PKN31_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98398.1"
                     /translation="MQILKNSSFLSQEMEDKEIFVLAGPNGVGKTEIALNLTSFFRSL
                     GLDSYLLDLDFTKADFTARSERFLSQVSILPGGKGKYADTPLWDKEIPEVLSQINKTH
                     RLVVDLGGDLKGMKLWRSLQSFWKNKQVHLTLVTNFCRPFSEEEFYFDFMERANTTLN
                     LEFHSVISNTHLIEYTDGEILKMGWQKAGDFSQKENLPIFFVSIWEDLIENLSWNIFD
                     SAVVGIQRFLHLPWMK"
     gene            13870..14082
                     /locus_tag="PKN31_00310"
     CDS             13870..14082
                     /locus_tag="PKN31_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA98399.1"
                     /translation="MAKAKVIINREFCKGCSLCVNICPKKVLTIENNFNSKGYYPATM
                     VDEEKCTACAFCAQVCPDVAISIYRE"
     gene            14086..15153
                     /locus_tag="PKN31_00315"
     CDS             14086..15153
                     /locus_tag="PKN31_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOA98400.1"
                     /translation="MERILMKGNEALAEAAIQAGCRFYFGYPITPQTEISEYLSRRLP
                     EVGGVFLQAESEIAAINMVYGAASTGVRVMTSSSGPGISLKQEGISYLASAELPCVIV
                     NMSRGGPGLGNIQPAQSDYFQATRGGGHGDYRTLVLAPSSVQELVSLTHRAFYLADKY
                     RNPVKILGDGMLGQMMEPVTWEAVELPSLPPKNWAIKGKNEGERKFILCMELDPRELE
                     NFNRKLERKYQQMAEEDSAWEIFGEEDNPDLLLVGFGTVARILKSVVREGKKEGWRLS
                     LIRPITLFPFPYNEVQEMAQKAGKVLVVEMNLGQMVEDVKLGVEGKAPVHFYGRVGGM
                     IPTVDEILQKIADILRGEGRK"
     gene            15150..15905
                     /locus_tag="PKN31_00320"
     CDS             15150..15905
                     /locus_tag="PKN31_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOA98401.1"
                     /translation="MRTIFERPKTLINKPFTYCPGCHHGLAQRLIAEVIDELDIAGKT
                     IGVGPVGCSVYIYEFFDLDFVMGAHGRAPAVATGIKRSLPDRIVFSYQGDGDIAAIGT
                     AEIVHCANRGELITSFFINNAVFGMTGGQMAPTTLPGQKTTTSPLGRNTEVQGFPFKL
                     GEMLARAEGSAYIARVALTNPRLIGQAKRCIKKAFLTQIEKKGFALVEILSPCPTNWG
                     MTPPEAVQWVEEKMIPYFPLGEIKVKEDSSHAS"
     gene            15895..16473
                     /locus_tag="PKN31_00325"
     CDS             15895..16473
                     /locus_tag="PKN31_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOA98402.1"
                     /translation="MHHEILIGGFGGQGILFLGRVLATAGMIEGYEVSWFPSYGPEMR
                     GGTASCTVIISEEEIGATVTDHPDICIVMNEPSFNRFSPLVKEGGLLVVNSSLIRENC
                     SRQDIDILEVPANELAGKEVGVAETTNMVILGALIARKPVVSFSAAEKALEKILTGKK
                     ANLLSVNFKALEVGKSFEKKGGVVSVSKRSDE"
     gene            16448..17107
                     /locus_tag="PKN31_00330"
     CDS             16448..17107
                     /locus_tag="PKN31_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOA98403.1"
                     /translation="MLVKDRMNREVVTVPPSTTIIEAQKIMKDKNIRRLPVVEKEELV
                     GIVTYNDLLEASPSKATALSRFEITYLLSKMSVAEIMTRKVVYVKPDTPIEEAALIMN
                     KNQIGGLPVVEDGRVVGIITESDIFEILVETLGVREGGTRLTLELPQRPGVLHEVTGV
                     IRKYEVNILSLATFYQQQKSDYRYVVMRLATREPEKIIGELNQNPDVNVYHVWVTPEE
                     E"
     gene            17110..17667
                     /locus_tag="PKN31_00335"
     CDS             17110..17667
                     /locus_tag="PKN31_00335"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOA98404.1"
                     /translation="MFFPYLENEDGFSLPQEISKEVLLETGIFKVVKNTYREKMERIF
                     IVHPGAVAISATTQDDKLLLIKQFRAPVKEWLWEIPAGTLEIDEEPLFCAQRELEEET
                     GFVSNNWQHLFPLVLAPGYSSEIIHFFRAKDASPVVEARAGDEDELIYFREVSRKEAQ
                     EMLDRQEIKDAKTRIGIYEWMKEKE"
     gene            17664..18845
                     /locus_tag="PKN31_00340"
     CDS             17664..18845
                     /locus_tag="PKN31_00340"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOA98405.1"
                     /translation="MRVLIALLDGVGIGELPDASFYGDEGSHTLRNTAQAVGGLYLPA
                     LEKLGLGWIDDIPGVKKIENPLAFTGKMKEKSPGKDTTTGHWEIAGIILEKPFPVYPR
                     GFPPSVIEKLERAWGRKILGNKPASGTAIIEELGEEHLKTGYPIVYTSADSVLQVAAH
                     EEVIPLSELYKMCQKAREIMQGEHAVARVIARPFVGRPGQFKRTANRRDFSLPPPQDT
                     ILDVLARYGKKNIGVGKIQDIFAGRGLSENIKIANNEEGFRVLRELQKSRKGDLVWVN
                     FNDFDTIFGHRNNPRGFAQALEDWDKELSSFLSFLREDEFLFITADHGCDPTTPSTDH
                     SREYVPLLVFNPCYSQGVSLGIRETFADVAATIAQMMEVEWNGPGTSFAFQILSGKNS
                     P"
     gene            18886..19617
                     /locus_tag="PKN31_00345"
     CDS             18886..19617
                     /locus_tag="PKN31_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOA98406.1"
                     /translation="MAIVAGSGWGEIVEGELINELSYSSLGIPFFTEVEGHAGKLRLL
                     KKNNLTFLLFSGRLHLYQGYSFFEATLPVLFSRLAGVEAIILTNSAGSLSFSLPPGSV
                     VLIDDQVDLTFYPDPILFSPPFFDKEIQEIFKKVGKEEGITLNQGVYVGVLGPSFETP
                     AEVRFLSRFGDMVGMSTVKEAKLASLLGLRIGGISLVTNWGAGMSSSFLHHQEVLQTA
                     ASLQPLLHRLFSSFLEEWGKNANRS"
     gene            19601..22207
                     /locus_tag="PKN31_00350"
     CDS             19601..22207
                     /locus_tag="PKN31_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOA98407.1"
                     /translation="MQIVVSHEGTDFDALASMFAVTKLFPGTKMVIWGTANRNVRHFL
                     SLYGNFFPILKEKEVDWEKVNKIYVVDTTYWERLSKAGELIRDGKVEVVFFDHHSEGE
                     NSEGEKKEGVVIIKEYGACTTILVEKIMEQKIALSPLEATLLALGIYEDTGSFTFSTT
                     RAEDLSAASFLLRQGAKVGFVNRFTGIQLTPEQRETLKKMINLLHVEEIEGIPVHFCR
                     MKLTEYVEGISLLVHKLMDLEEIELLFVLMETENKVYVIARSRLEEVDLAKILGKMGG
                     GGHHSAASLTVKGKKIEEVEEMIREALSASLSFTRAKEVMSCPVKVISPSTTIGEALL
                     ILMRFGFSGLPIVDENGKLVGMIARRDVEKAFRHGLQNAPVKSFMSSQLVTVDPEDSI
                     FKVRNLMVEKDIGRIPVVRGDTLLGIITRSDLLRVLHQRENSVVQELTRLNLAEKLYL
                     HFSPLDLKLLHFIGDESKRAGAKAYLVGGVVRDIILGFPGYDLDIVVEGDAIELAQTI
                     SRKLEGKVVSYPPFGTAILFLKDGKRVDFASARSEYYDSPGAPPHVEYSNLRRDLFRR
                     DFTINAMAISIGTDDWGDLFDFFGGLKDLENKTLRVLHPLSFVEDPARAIRAVRFEQK
                     YGFEIEPFTLSLLKQTVREGLLNKIKPDRLKEEINLILRLPDFYRLLERLYQLDMFPY
                     IFPGSTWRREFEPVYQRLVKLLDRFSFLSLDYFLAKLSPLWGEMDPAYLGELKERLAL
                     SKASIRKISTYLDKKEELEEKLPDETLPDSQAYLLLRSLGDEFLLLLEAKWGEDSLPG
                     RRVEQYLKEWRKITPYLTGEDLRRKGIPAGPIYAKILEELKLARIDGLVKSREEEEIL
                     VEKIWRNEAHER"
     gene            22197..23198
                     /gene="trpS"
                     /locus_tag="PKN31_00355"
     CDS             22197..23198
                     /gene="trpS"
                     /locus_tag="PKN31_00355"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOA98408.1"
                     /translation="MKDKETIFSGMRPTGKMHIGHYFGALKNWVELQEKYHCIFGITD
                     WHALTTSFEDTDQLENNIIEMAADWLAVGIDPEKCVLILQSWVPEHAELHLLFSMITP
                     VGWLERNPVLKEQLQDMGLKEVVGYGHLGYPVLMASDILIYKAVKVPVGEDQIPHIEI
                     TREIARRFNYLYGEVFPEPQALLTEAPRILGIDGKKMSKSLNNYIGLSEPPEEVKKKV
                     HSMFTDPEKIRLSDPGHPERCNVFSLHRLLTNPRVLEIEKDCREGKWGCVSCKEELGE
                     LMVEFLEKVVPHREKYQQEKHLVKEILWEGSKKARQLARATLEEAKDAMKLKYDFKS"
     gene            23182..23790
                     /locus_tag="PKN31_00360"
     CDS             23182..23790
                     /locus_tag="PKN31_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98409.1"
                     /translation="MILNPESQWKKLLQETPFPQCIGVWEEILTFLENQLDEALGKET
                     EEKEEANLLQEREERFLLFTQLLSSLEEREENTLKAPEKRWEAIQNREVKEIEKLTLA
                     ELLSLYELYLTPETPPPDVSLDSQFQKLLGERKMVIKKMVREKEFRDMDEFFYDCPDR
                     KATIATFLILLDLVFRKELQMRRGEDGRIYFGKTYPPVKEPN"
     gene            23753..24301
                     /gene="scpB"
                     /locus_tag="PKN31_00365"
     CDS             23753..24301
                     /gene="scpB"
                     /locus_tag="PKN31_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOA98410.1"
                     /translation="MEKPILRLKNLIEALLFSSPHPVSCSEMVKLTEADKRTVIQTLQ
                     ELQDEYEKREGALAIREINGKWQMIVKPEYGQELKEKMKVSSTKTISRKALETLAIIS
                     LYQPLTKTQIDLKRGVDSAQTIRTLLERGLIAIAGRSDLPGKPFLYKTTDKFLEVFGI
                     RSEEELERLKNLFWEKKNGETK"
     gene            24285..25013
                     /locus_tag="PKN31_00370"
     CDS             24285..25013
                     /locus_tag="PKN31_00370"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOA98411.1"
                     /translation="MERLNKFLARAGVASRRKCDQLIKEGKVTINGRITTNPAERVSG
                     KEIIKVENKVVKKEELVYIELYKPRDYLTTLYDPRGRRTVADLLSGVTTRVFPVGRLD
                     SESEGLLLFTNDGALSYFLTHPRFEVEKKYLVYVEGKIEKRGLSILKKGITVKDIPYR
                     IRRGEIKEIYSNCSVVEVTLLEGKKHEIRIMFDYLGHPVLRLIRISMGPLVLDKNILP
                     GEWRFLSDEEVQLLQKYQEEKLGR"
     gene            25003..25524
                     /locus_tag="PKN31_00375"
     CDS             25003..25524
                     /locus_tag="PKN31_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOA98412.1"
                     /translation="MADKSGLIQVYFGEGKGKTTAALGTTLRAAGQGMKVLLVQFFKN
                     KFTGELASLQLLPGVDVYRFGSGEFIRGEVSPEDQREFFLGWHMVREALEKKEYDVVI
                     LDELPYAFYYHLLTWEECREALEQKNPEVEVIITGRKVPEELITIADLVSEIKLVKHP
                     FNKGIPARRGFEY"
     gene            25526..25909
                     /gene="aroH"
                     /locus_tag="PKN31_00380"
     CDS             25526..25909
                     /gene="aroH"
                     /locus_tag="PKN31_00380"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOA98413.1"
                     /translation="MEKKVRGIRGATVVEKDDKEEIKKAAKELFDLLLRENNISPSEI
                     VAVFITATPDLHSTFPAEAIRERDEFRYIPFICAQEMRVEGALEKCIRFLVLAYTDKD
                     QIDIHHLYLGKASSLREDLSRRENL"
     gene            25906..27228
                     /gene="aroA"
                     /locus_tag="PKN31_00385"
     CDS             25906..27228
                     /gene="aroA"
                     /locus_tag="PKN31_00385"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOA98414.1"
                     /translation="MKEVLIHPFSQKVEGEICAPRDKSLSHRAAMIGALSQGTTVIDG
                     FSFCADCFSTLSGLELWGVEIIKEEEKERVTIKSEGLGKLQEPEDVINTGNSGTTMRL
                     LAGIATGIEGLTIFTGDESLRRRPMKRIMEPLRLTGVEVGGRKGDSFPPFFVRGKNRV
                     KAFSYRLPIPSAQVKSALIFAALRGEGTSFIEEPLPSRDHTENLLCYSGVKIEKRGGV
                     IEVNPPSSLSASYLSLPGDLSSASFLIALTTLLPRSRILIKDIGLNPTRAGFLTCLRQ
                     MGGNFKIIGKREEFGELRGDIEVESSSLQGVKIGKDLVPLLIDEIPIIAVLAAKARGT
                     TIISGAEELRVKETDRLKAIAQGLKTLGAEVEEREDGLLIQGPCVFQGGRVNSYGDHR
                     IAMSLVVAGITGERDLVVEDIDCVKISYPGFFSDLEKIGYKEWEIRER"
     gene            27225..27881
                     /gene="cmk"
                     /locus_tag="PKN31_00390"
     CDS             27225..27881
                     /gene="cmk"
                     /locus_tag="PKN31_00390"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOA98415.1"
                     /translation="MKKYIAIDGPAGAGKSTVAQKLAQSLKYLYLDTGALYRAITLYF
                     WENNIPLEDCKILTSVLSRIKLEVSWEEKFRIFLNGREVTEDIRQPEVDALVSPVAKI
                     KEVRNYLCVVQREIALSRPSVTEGRDIGTVILPDADLKIFLTARPEIRAYRRWKERKD
                     KGNNVDYKVVLKNVLERDFIDSTRDDAPLKKAEDAIEVDTSFLSVDEVVEKLYKLVEN
                     "
     gene            27883..28815
                     /gene="ispH"
                     /locus_tag="PKN31_00395"
     CDS             27883..28815
                     /gene="ispH"
                     /locus_tag="PKN31_00395"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOA98416.1"
                     /translation="MEVWTANRVGFCPGVKRAFDLALRALSREKPVYFLGELVHNSQA
                     VKMLEEEGGIIVTDPEDIPPFSLVVTRSHGLEKRIKEFLESRQVKIIDTTCPRVKKVQ
                     ILAQELEKKGYNLVILGSSKHPEVRALLSFISQKALVLEKKEEWGKLRNFSPQEKLAV
                     VEQTTFPREIKEEFTQEIKEEFTHLNLEVFDTLCAETEMRQRDLVESIKLHKVNKVVV
                     VGGKNSSNTKALFLTARRLGIESIWVERVEELEEGYGDKEDRILVVSGTSTPHFSVED
                     VVKKLKAREASFYERKSADGSHHRENKCGEIHSI"
     gene            28748..30079
                     /gene="der"
                     /locus_tag="PKN31_00400"
     CDS             28748..30079
                     /gene="der"
                     /locus_tag="PKN31_00400"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOA98417.1"
                     /translation="MKEKVPMVAIIGKTNVGKSTLFNHLSRKRVATVDSHPGVTRDFL
                     ENIVDIEGKKIKLVDTGGLEILVDSQNEMQKKIEEKTWEVMEKADLLLWVLDGRENLT
                     SIDWELASRLRRIKSPVIIVINKREGMTSEAPPAEFFSLGFEPIIQISATHGDGLRQL
                     RETIAELIVGNTEICFDRHRIPVAIVGKPNVGKSALYNSLLGKERSIVSDIPGTTRDA
                     LLSTLDTSFGYYELMDTPGSIARSKIKERIDFYAALRTEESLQDAVIALLVIDPIQGI
                     TRQDKRIAQKIKEDKKGCLVFINKSDLVLNHHSEWQVKKLVEEVRRELSFLNYASFLV
                     GSAFSPSVSSDILSVLAGIATRYYQRIKTSFLNEKIREKINEEGWKVGGRTFRTYYMH
                     QKDIAPPRFYLSVNFSGTKREEELLVNRIEKLIRKEGDWEGVPISIELSRE"
     gene            30060..30983
                     /locus_tag="PKN31_00405"
     CDS             30060..30983
                     /locus_tag="PKN31_00405"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOA98418.1"
                     /translation="MNSPANEKIIHIFLDYLQKERRYSPHTVDNYGRTLRQFSKFLEE
                     RNLTLVEAKTQDIADFLAYLKTHHQLKRISQANRISALRTFYRFLRRRGMVDCDPTDG
                     LGKLNKEKKLPNFLTVGEMEFLLNYLKQKAREKSDFPSLRNWAFFELLYSSGLRVGEI
                     TELKTKDLDLTSKMVKVKGKGGKERLVPFNESTQKALRKYLNHRPFPREELFLNQRGA
                     PLTPRGARKILETIVKEAGLGGKRISPHVFRHSFATHFLGGGAELRMVQEALGHSSLS
                     TTQIYTHLNWDKMKRIYQETHPHAKKVKNDD"
     gene            30976..31503
                     /gene="hslV"
                     /locus_tag="PKN31_00410"
     CDS             30976..31503
                     /gene="hslV"
                     /locus_tag="PKN31_00410"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOA98419.1"
                     /translation="MIKSTTVIAVLKNGKAAMACDGQVTMNETVLKGGARKIRRIYQD
                     RVLAGFAGSGADGLTLLERLEEKLNYYSGNLPRAAIELAKSWRTDKVLRQLDALLLAM
                     DKEHILIISGRGDVVEPDEPVAAIGSGGNFALAAAKALWRETDKEAEDIARISLQIAS
                     EICIYTNSNMTVETI"
     gene            31519..32898
                     /gene="hslU"
                     /locus_tag="PKN31_00415"
     CDS             31519..32898
                     /gene="hslU"
                     /locus_tag="PKN31_00415"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOA98420.1"
                     /translation="MSLIEENLTPEEIVEELNKYIVGQDEAKKCVAIALRNRIRRRML
                     PPDVAEEITPKNIIMIGPTGVGKTEIARRLAKLTNAPFFKVEATKFTEVGYVGKDVES
                     IIRDLTELAVRMEKNERIKNQEEKAKEIVEELILDYLLAFNPSSLEYEDTVTAESPRV
                     DVVWPKPLSSTREKLREKLKKGELEERFIEVEIEESSIPLGIIAGGGMESLGIDFQEL
                     FSGVFPSRKKKRRVKVKEAREILFNQEVQKLIDMEEISTEAIKRVEETGIVFIDEIDK
                     IVSPGGNYGPDVSRGGVQRDLLPIVEGSTVLTKYGPVRTNHILFIAAGAFSHTKPSDL
                     LPELQGRFPIRVELSSLSEEDLYRILIEPFNSLTKQYKALLETEGVSIEFEEDALREI
                     AHISQELNQRMENIGARRLHTVMERLLQDISFRAPRLKGQKIVINRDYVLQELADILQ
                     DEDVTRYIL"
     gene            33037..33963
                     /gene="rpsB"
                     /locus_tag="PKN31_00420"
     CDS             33037..33963
                     /gene="rpsB"
                     /locus_tag="PKN31_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOA98421.1"
                     /translation="MMVVTMKQLLEAGVHFGHQTRRWNPKMKPYIFAERNNIYIIDLQ
                     KTVELAEKAYNFARDLSREGGMFLFVGTKKQAQEAIEQEAQRCGMFYVNQRWLGGMLT
                     NFSTIRQSIERLKKIEEMEEKGILERLPKKEVMQIKKRKARLEKYLSGIRDMEKLPDA
                     LFVVDPRRERNAVLEARKMDIPIIAIVDTNCDPDEIDYPIPGNDDAIRAIRLFSALIA
                     DAVLEGKRLASEGQEMIATEGIAIPQEEIEEAEVAEEIEEIVVDNNDNKEEEENVSQE
                     EDALQILEELQDSGLEEIEDKETEKEEDDDNS"
     gene            33960..34562
                     /gene="tsf"
                     /locus_tag="PKN31_00425"
     CDS             33960..34562
                     /gene="tsf"
                     /locus_tag="PKN31_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOA98422.1"
                     /translation="MSATAKEVFELRNLTGAGVMDCKKALEEAGGDIEKACDILRRKG
                     LETAAKKQGRVAQEGLIGAYVHTDGKIGVLVEVNCETDFVARTDEFRYLVKELTLQVA
                     AQAPRWVAPENVPEEIKKKEEEIYREQLKDSNKPEEVKQKIVEGKMKKFFEENCLLEQ
                     PYIREPEKKVKDLLMETIAKLGENIVVRRFVRYKLGEDNE"
     gene            34555..35298
                     /gene="pyrH"
                     /locus_tag="PKN31_00430"
     CDS             34555..35298
                     /gene="pyrH"
                     /locus_tag="PKN31_00430"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOA98423.1"
                     /translation="MSDQQLSSLRFKRILIKLSGEALMGSLPFGIDTQVLGYITKEIE
                     KVKNVGAEICIVVGGGNIFRGRTAGDKGISRVTADYMGMLATVINALALQDFLENEGI
                     PTRVQTAIEMRAVAEPYIRRKAIRHLEKGRIVIFAGGTGNPYFTTDTAAALRAAEVRA
                     EALLKATKVDGIYEADPLKEKNAYKFDRLDYLEVLNRGLKVMDSTAISLCMENNIPII
                     VFNVNEPDNLVKVVTGHKIGTLVGREGEK"
     gene            35295..35861
                     /gene="frr"
                     /locus_tag="PKN31_00435"
     CDS             35295..35861
                     /gene="frr"
                     /locus_tag="PKN31_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOA98424.1"
                     /translation="MNEDLKLVIKDVEKRMKQAINVAREELSRVRTGRASPALVEDLE
                     IDYYGTIMKLKQIASITTPDARTIIIQPWDKNALSLIEKAIWKSDLGLNPQIEGGVIR
                     IAIPPLTEERRKEIAKIAKKWIEEAKVAIRNLRREANEKIKKMEKRGEISEDESERAQ
                     AEVQKLTDEHINDLDGLWEKKEKEIMTI"
     gene            35825..36553
                     /gene="uppS"
                     /locus_tag="PKN31_00440"
     CDS             35825..36553
                     /gene="uppS"
                     /locus_tag="PKN31_00440"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOA98425.1"
                     /translation="MGKERKRNNDYIGTILRHVAIIMDGNGRWAEKRGLPPLEGHRKG
                     VEVVRILVEETAKVGISFLTLYAFSTENWKRPVNEIQGLMRLFVESMDKYGKELEDNK
                     VRVKFLGRKDGLPEDLVLQMNDLEKHTMRGENLNLNLAINYGGRDEILRAVKNIYQEN
                     HSLLASLLEENFSAYLDTGNQPDPDLLIRTGGERRISNFLLWQIAYTELWFTDTLWPD
                     FTIEEYHQAFQDFMERERKFGGRF"
     gene            36577..37359
                     /locus_tag="PKN31_00445"
     CDS             36577..37359
                     /locus_tag="PKN31_00445"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOA98426.1"
                     /translation="MRKRTWSVVWALPLFLFLILSSSWTLILVFFFLSFGSLREYGRL
                     VHEREEWTSWMCFSLVSLVLLYLSLTGIDYKAILFLGYILFLMLIGWGLKKPQRMVER
                     VAFYLLGFLYCSFFPFFWVKMGITYSRISIVFFALPVWINDIFAYLVGRKWGKHKIVP
                     QISPGKSWEGLAGGIGAATIMGLVLTTPSISFSLSQGLLTGFLIALVSFLGDIWESAL
                     KRRAGVKDSGSFLPGHGGILDRFDSFFLVGPLVYLLRLSWGG"
     gene            37365..38498
                     /locus_tag="PKN31_00450"
     CDS             37365..38498
                     /locus_tag="PKN31_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98427.1"
                     /translation="MKVAILGSTGYLGEQILEVLEKRKDFEVILLSGYSKEERLREQA
                     QELNVSYAFNPRGRKSLWANSSTTLITDYNLLEKVILQEVEGVFFAASGIEFLDLFWK
                     LIDTSRILWVASKEIIILTGELKSHLLKGRENLIPLDSEHNALWQILKGVEREEVSRV
                     YLTASGGAFYEWQGSWDDITPEMALSHPNWKMGVKITIDSANLINKGLEVIEASALFG
                     FSSEEIEVAIQRESFIHALVELKDGFSLALLSPPDMRSVVLNAISGKKRETNPFQSLD
                     FSQIKSLSFDYPVPEKFLGFYLAKEAGRRGKGYPALFCGADEALVSAFLSREICFGDI
                     PLILEKILEQEVKPPESLEEVKAIYYQGYKLAQMLILERKNIQ"
     gene            38495..39547
                     /gene="rseP"
                     /locus_tag="PKN31_00455"
     CDS             38495..39547
                     /gene="rseP"
                     /locus_tag="PKN31_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOA98428.1"
                     /translation="MIITILAFIFVISLLIIVHEFGHFIMAKWCGVQVLSFAIGYGPK
                     LVSFKSGETEFSIRLIPLGGFVKMGGMEGNVIEGYEEVSIPPEKRFDHKPLWQRSLIV
                     AAGPIMNIVLSIVLLFIVFSVTGIPAGNLKVQEVVAGGPADRAGIKVGDVILAVNGRS
                     ASNLDEVVSIISSHPGEEIELTVKRGEEEMILKVIPEWNETEKRALIQVVFGIENKRL
                     NPFLTLFHSAKTTISWFVLTALGLLYVILGKIPAEMAGPLGIAQIAGQAAQLGFLNLL
                     MFSAVVSVSLALFNLLPIPVLDGGYLLLFLYEKIRGKPLEPEKVGWIYFIGIILIFSL
                     AIFVTYQDIMRIVAGK"
     gene            39544..40614
                     /gene="ispG"
                     /locus_tag="PKN31_00460"
                     /gene_synonym="gcpE"
     CDS             39544..40614
                     /gene="ispG"
                     /locus_tag="PKN31_00460"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOA98429.1"
                     /translation="MRRENTKEIPLGKIKVGGKNPIWVEAMGRENPAKIRESIREIRE
                     AQEEGCEIFRLAVPDEESLFGLSQIKKQVDLPLIADVHFNLELGIKAAQLGIDAVRVN
                     PGTAGNKEKLEELIKVLHDKNLALRIGANTGSLPLHYREKNRVEALFDSVEELISITE
                     KNNFRNIILSAKSTDIEETIAIYDKLSRAFSYPLHVGLTEAGEGEEGIVKSSIALGII
                     LREGIGNNIRVSLTAPDPVSETRVAWSILEALKLRSKGLEIISCPTCARRKGDVVALT
                     RDFKRKVKERKIKIVGKVAIMGCEVNGPGEAREADWGLAFAPRGKALLFSRGERIKMV
                     KQEEAEEELIRLITKEMEEKKP"
     gene            40617..41867
                     /gene="proS"
                     /locus_tag="PKN31_00465"
     CDS             40617..41867
                     /gene="proS"
                     /locus_tag="PKN31_00465"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOA98430.1"
                     /translation="MSSLFVPTLKENPQEAEIASHSLMLRAGMIRQLSSGVYIFLPLG
                     LRTLNKITKIVKEEMDRAGGQELLLPALHPGELWKETGRWEIYGPELIRLKDRRDRDF
                     CLGPTHEEVITDLARREIRSYRQLPLLLYQIQTKFRDEIRPRFGVMRAREFIMKDLYS
                     FDRDFEGLRVSYEKMYNAYCRVFSRCGLSYKIVLAESGVIGGEVSHEFLIPADSGEEK
                     FLVCPACDFGTTQEEAKCPNCGDSLEEKRGIEVGHIFQLGTKYSESMKAYFVDKDGEE
                     KPLIMGCYGIGIGRTMAAAIEACHDEKGIQWPLSISPYEVVIIPVNWRKAEQRKAAEE
                     IYQELKKRDFEVVIDDREFSAGYKFSEADLIGFPLQVIVGEKMSQEGKIEIKVRQNNE
                     RWETKKEEVLTLLLKLKEELAPKV"
     gene            41880..42536
                     /locus_tag="PKN31_00470"
     CDS             41880..42536
                     /locus_tag="PKN31_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA98431.1"
                     /translation="MNVSINAKATAIIAAYNEEKTIGPIIDVLKAIPFLNQIVVVSDG
                     STDHTVDVARSRGVEVVELLYNVGKGGALYQGLMNTTSNVVLLLDADLIGLTHQHVID
                     LVKPVIDGEADVTIGIFEDGRFATDFAQKIAPFLSGQRAIRIDILQNIPDIEVSRYGV
                     EVAINRYIRKNGVRVKLVKLPGLTQVMKEEKLGLIKGLRERMKMYWDIIKNFYRGGDK
                     "
     gene            42540..43808
                     /gene="hflX"
                     /locus_tag="PKN31_00475"
     CDS             42540..43808
                     /gene="hflX"
                     /locus_tag="PKN31_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOA98432.1"
                     /translation="MEEKRGILVLVDQGNNRSFSSWEGEKREIEEVAWSAGWKILAEV
                     ESRVKKPFYKYYLGKGKVEEIKSLIEDNPEANFILFNVEITPSQQRNLEETWQVPVYT
                     KNALIHEIFAQRARTAQGKIKVELAQLRYQLSRLTGKGRELSQTGGGIGTRGPGEQKI
                     EVERRKIKDRIAHLNRELQKIERQRDVQKNLRRARKIPNVALVGYTNAGKSTLLNALT
                     GSQVYVEKKMFATLDPTTRKLFIPGVGETLFTDTVGFIRQMPTTIKDAFRATLEEIKD
                     ADLILEVIDISDEDYYLHFQVINQILEELGINNSPRILVFNKIDLLQSPLVLEREIEE
                     NYVYAFISAQKTTGLKELKEKISLSLQHEVRHLCLQVNHSQLPLIQEEIYQQGYIEEL
                     KTMKDGEVMIKCVVRKEAAAKICDLSEVRF"
BASE COUNT        13936 a         7329 c        10313 g        12489 t
ORIGIN      
        1 ttcctcgacg ttcatcagtc tctttttcgt agccttctac acttatagcg ggataaacgt
       61 tacccatttg ggctaagcgt ttagccactt cctgattaat caaagtaccg ttagtataaa
      121 tctggaaagc tatatcgggg tgtttttcaa aaagatctag gtgttctttt cgaatgaatg
      181 gctctcctcc agaaatagtg aggaaattca tgcccatttc ttcggcttca ttgaataagc
      241 gatccagaag ctcgatgggc atatcacttt ctttgcgata ctgcccagca taacaaccat
      301 aacagttaag attacatctc atagtagggc ttacgacaaa gaaaaaagga acctgtactc
      361 ctaactcttt ttctaatttt tgtcttttgg gaactcccaa aagaatgctc ttaacgatga
      421 gattaactac gagtttctct cttacattgg gatgagtttc ttgtagaaca cggcgggcta
      481 acactatctg gggtctttgt tcttcaaaca tttgacgaag cgctcgaatt tggtctcgat
      541 aatactcaat aggagtaagc ttctccgcca gataggtgag cctgatgatg ttttcatcag
      601 aagcattgcg caaagagttt accattagct ttactacttg ctcagtagtg aatgctttta
      661 aggaatctaa ataactcata ttaagtccct ccctcaaaag gaattttttt tattataaca
      721 cattgcctaa gaactttgtt tcaaaaggtc atatgttctt ttaacctctt gaatgtcttt
      781 ccaggttaaa tatcgaggag atcctggttg tcgagaatta taacggagga gaaagctggg
      841 atggaacatg ggaaagattt tttttcctcc tcgccaatca aaccactgac cccgaagttt
      901 gctgataggt tcttttttgc ctaaaaggta aagagtagaa gtgccaccta aggttatgat
      961 tatagaagga ttgataatag cgatttgagc ctccagataa ggaaaacaac tatccatttc
     1021 gctttgagca ggggttctat tgcccggggg acgacacttt attatattgg taatatatac
     1081 ttcttctctt tttaaaccta ctgattgaag gattttagtg agtaactggc cggctttacc
     1141 tacgaaaggt cttccggttc ggtcttcttc ttctccaggt gcttctccaa cgaacataag
     1201 caatgcatca gggtcccctt ctccaaagac tattttagtt ctgctctgag aaagggaaca
     1261 aagggtacat gtttttacct cgctttttat ctcttctaat aattcttctt tgctgatcga
     1321 ggactttatc actttttcac ctgcaaaaca aagatacact tcttaattca gctattacta
     1381 ctaattttat catataatta agcttttcaa aaatttttcc gataagagaa tatctttaca
     1441 tctttttaag tcaggagcat tccttgtcaa ataactgcag ccctctgcat aaggacttat
     1501 atagaacaga actatgggga aatttcacta accactcctt taaagactaa acgtaacttg
     1561 ttaaaaactt attctactca tcctcaattc ccctatctat cgtctgccct ctgcaatagc
     1621 tttcgtaatc gatatagttt tagcctgctt ggatatatca ggacgatgga gatattcagc
     1681 actagataga ggcatccagt tatacgtgtt ttccaatcaa gagaaacagc ccatgtatta
     1741 gtagtggtaa cctttcctgt tgtaagggag caatagtttt ttctatatgt agcgtgatga
     1801 atagtagttc tcttactcct tgtctcaaga tataatttcc gctcgagtgg aagcgaaaaa
     1861 ctggaattgc ttctaccagc cttttcttta ccagagaaca gttttttgct tttagcgctt
     1921 tgctttattc aggaagtaag ttggaagaaa gcgattctat catttagttg gctcaataat
     1981 gctatctttt ttgagacttt ctatatcctg gaagagagct tttttatcct ccggcattaa
     2041 gatctttaaa gttaaaagag atagcctgct ctgggcagaa gacttaatta ctagagaaat
     2101 gaaggatgac cctatcgcta atagacctgg aagtggtaat tgtaattttg gtaagaaagg
     2161 ggaaattgaa aagcaggtaa aatagtttct tctgatatag aggggctgct tagctctctt
     2221 ttagccatcg tttctctgca actcttggat tacttatata gttctggaga gaagatgtaa
     2281 tacaggctaa cctcgtcact taattaaaga cgtgacggta gaataataat cacttttctt
     2341 cgctaagagg ctctatgtgg actattccct caaagcccgt ttgttcttta atttcttttt
     2401 ctattttttc ggacagatca tgcccctctt taactgaaag gcttccatct acttcaatat
     2461 gcatagtaat aactccttta cctccgtaat catggaggtg aatagagtga acattatcta
     2521 cttccttaaa ttgggaagtt atattcaaga tcttctcctt ctcttcagga gaaatgcttc
     2581 ttcctaacag ccgagaaacc gagccccgca aaattttgct agccatcaag atgataatgg
     2641 tagaggtgaa taaactgcta actccatcca accagggata acgaggaaac aaaaacacta
     2701 ctaaaacagg cagagagtct aaagcgtcac tacgatgatg ccaggcttta gcgatgagaa
     2761 gttcggaatt agatgccttt ttccccagaa aaaaggctac ctgcgccaaa atttcttttc
     2821 ctcccatgcc tattagtatg gcgaaaagcg ccggtaaaga aaactgaata ggagaaggat
     2881 gaaaaatcct ctggcaacct tgaattaaga attgaatgcc tacaataaaa agaagaacgg
     2941 ctataataag agtagtggta tcttctaacc gaccatgccc gaaaggatgt tctcggtctg
     3001 ggggacgatc gagctctcgg gcaatgaaga aaatggctaa agaagtagct acatcggaca
     3061 atgtttcaaa agcatcggca gtaagagcta aactttgaga aaggcggcca tagtaaagtt
     3121 ttatcccaaa aagggtaaaa ttcactaata tactgatcca ggcctctaat ctggcgtagt
     3181 aagccttgga ggccagagga ttactgggaa tttcttctga agaaaatttt tgctctctca
     3241 ttttccactc tttggttttc gataaaataa tcgagggttt tctttgcctc tatattataa
     3301 tcttttaagt ttcactgtaa agctattgaa aatagcggta ctttccctgt atctgttata
     3361 atcttcctgt cctgtatagt gttagcgatt gttagaagcg ttagaaaaga aagcacatcg
     3421 atgattgatt cctgtagagc tttatctcct taaaagggag ggtagaaaat ggccagaaaa
     3481 atggggaaga aattacaaac cgaggttagc tcagaatccg gtatgataga tatcttcatc
     3541 ctggtagtag ttagtttttt acttattatt tcttcctggt ttcttggcaa tgtgcttctt
     3601 cattatcaag tagtagatgc tcacattgag aaattcaatg aggaatataa aatagaggga
     3661 gcattggtgg aagctatcca gctgctgaaa gataaaggta gtactttcca gggggataat
     3721 atcccttctt cttacagctc tcttttttac ttttcagtag aagagggtaa actgattatc
     3781 agtaaaaaga tcgaaggtca ggagaaagaa gaggagaaca tgctacctgt ggctgaggtt
     3841 acttttacct gggaagaaaa tgagttgaaa attaaccgaa tagagacgag atataatctt
     3901 ccttattcta aataaactta ttgatggagt actgaattgg aaaacaccaa taaagataaa
     3961 accaaagaaa taaatcaaag gggtcgtggg aacattgccc ttcttggttt tatgggtagt
     4021 ggaaaaacca ccatcggaca gcaattggct caaaaagtag gatggcagtt tatggatacc
     4081 gatgatttaa ttgaagaaaa gacttctcta tctatctctc taatttttga acgatggggg
     4141 gaagagcatt tccgctcttt agaaaaagtg gtactcgaag aagtggcttc aaaaaagaat
     4201 ataattttag ctacaggagg aggacttcct gctcaagagg ataactggca gatattaaga
     4261 gaacatttct taaccatttt tctgcaagtt gatttttcta tcctctatca gagaataaaa
     4321 ggagattccc gccgtcccct tctatctcag tattctactc ctcaagaatt agaaaagctt
     4381 tatcaaaaaa gacttatcta ttatgagagg tcagatgtta taattaatgg aaatagaagt
     4441 gaagaggaga tagtagagga aatactatac catgagaaaa ttagcagttt gtggagaaag
     4501 cggtaagcaa tacccggttt ttttaggctg ggatttactg gagagagatg attggttaga
     4561 agagataaaa ttcccttctt ctttcggggt gatagtaacc aactcccggg tgggatctct
     4621 ttatgaggaa agggtatctt cttttttctc ccggcaagga ttcactattc aggtagtcac
     4681 cattcccgat ggagaagaat ataaaaatct ttccacggtg gaattcattt ataaacagct
     4741 tctaaattta gatttagacc ggaagagttt tctcttagct ctggggggag gagtgattac
     4801 tgacattact ggttttgtgg cttccacctt cttacgggga ataccttatt tttctattcc
     4861 taccactgtt cttgctcaag tagatagttc cataggaggc aaaaccgggg taaatttgcc
     4921 ccagggcaaa aatctggtgg gaacttttta tcctcctgcg ggggtattca ttgacctttc
     4981 ttttcttcgt actctccctc ctcgagagta taaggaaggt cttgcagaag tagccaaaac
     5041 tttcttttta gctggaggcg agattctttc ccttttagaa aagagagaac aaattctttc
     5101 tcgagaagaa gaaattttgg aagaactaat ttttcagtct ctttctttaa agaaaaaaat
     5161 agtggaagag gatgaaaaag aaaaggggat acggagtatt ttaaattatg ggcacaccat
     5221 aggtcatgcc ttagagaaag tagaaggcta tggtaagctc cggcatgggg aagcgatcgc
     5281 tttaggaatg atgggagagg ctatcctggg agagaaatta agcattactc cttctaaggt
     5341 agtagataaa caaagagaaa tattagctaa tttctattta cctactcagc taaaatttaa
     5401 gatagaaaga gaagaatttt ttgctgctct ccgtcaagac aaaaagaagg aaagaggaaa
     5461 agccaggtta gtttttttga aagattttgg tcaacctatc tggggagtag aggtagaaga
     5521 ggaaaaaatt ggagaagcgc tttcttattt agagaggtga ggatagcttg aatattctct
     5581 ttatttgtgg tcctaatctt aatcttttgg gggagagaga aaaggagatt tacggagaaa
     5641 aaagttttga ggagataaat atcatactgg aaaaagaagc tcaaaaaatg ggagtgaacc
     5701 tggagatttt tcaatctaat catgaaggaa atatcatcga taaaattcag gaaagtagag
     5761 gtagtgttga tggtataatt ataaatccag gggcttacac tcattatagc ttagctattc
     5821 gcgacgctct aaaggtagtt aaagctccta taattgaagt gcatttatct aatatatata
     5881 gtcgggaaga atttcgtcat cactcagtga ttgctccggt atgctgggga cagatagcgg
     5941 gtttaggata tcagggatac attctggctt tacaagcttt tttgatgagg ggaaagaatg
     6001 aaccataaaa aaagaatggc tcaattaaaa gaggatttag agcatccttt tttagttaca
     6061 gaaagagagg acctttttta tcttctgggt ttccagggtt caacaggttt tctcctggtc
     6121 tttcctgata agacaccagt ttttttctgt gatggccgct atgctaccca ggccaaagaa
     6181 gaaatcaaat tagaagtaga aatccaagaa ttcagagatg aaattactaa aaacataaaa
     6241 gaggcagtat ctggaaacgg ttatcaaaaa cttctggttg aagatagctt aaaaataagg
     6301 ttttacgaac tccttaaaaa ccagggctta gaggttgctc ctgtttcttc cccctctcga
     6361 caaatgagga tgattaagga tgaagaggaa atcgaggaaa taaaaaaagc ggttagaatc
     6421 tcagaagaag cgctaaaaaa agtaaggtct cttttccgag aaggggtaaa agaaaaagat
     6481 atggctttag agttagatta ccagatgcgc ctgctgggtg gagaagagac agcttttccc
     6541 accattgtag caggggggaa gagaagcgct ctccctcatg ctcgcccttc ttcttattcc
     6601 tttaaagata atgattgggc aattgttgat tggggagcac gggtcaatgg ttactgtgct
     6661 gatcttactc gagttatagt cataggggaa gttgattctc gcttttacca gcttcttcga
     6721 atagtagaga aagcccagca actggcagaa accattataa aggaaggagt aaaagcaaag
     6781 gaagtagata aagtagtgag aagctttttc aaagaagaga aggtcgagga gtattttaat
     6841 cacagtttag gacatggagt ggggatagaa attcacgagg accctcgctt gggccctaaa
     6901 agtgaggata ttttaaaaaa gggtatggta gtaacagtag aaccaggaat ttattttcct
     6961 gattggggag ggattcgcct ggaaaattta gtagtggtgc aagaagaggg aaataaaatt
     7021 ctggattctc ttcccctatt actttgaaag gaggaccaaa gtgcctgaat acatttccag
     7081 caatgacttg agagtaggaa cttgtataga agtagatggt aatctttatg tagtcacctc
     7141 tttccagcat atcaaaccag gtaagggagg agccataatt aagaccaagc tcaaggacat
     7201 taataccggc tttataatag aaaaaacctt tagagctggc gagaaaataa tcagagcgat
     7261 actggaagtt aaaaaagctc aatatatgta tgaggatgca gccggatatc atttcctgaa
     7321 cttagaaagc tacgaagaaa ttgttttgcc cgaagagatg gtgggggaaa accgagactt
     7381 tttagtggaa aacctggagg tggaaatact gacttaccaa ggaaagtgta taggtgttga
     7441 actgcctccc tttatagaat tagaggtagt ggaaactact ccgggaatta gaggagatac
     7501 agcttccggc ggttcgaagc ctgctacttt agaaactggt gtagtagttc aagttcctct
     7561 ttttgttaac gaaggagata aggtgcgggt agatactcgt acccgagaat atttagaaag
     7621 agtatagctc gaggtgatca gttctgaaaa gtgcggaagt ttcgctacct gaaggacaag
     7681 ttctttattc tttaaaagct ttgcaagctt tagtggatga ggtagttgag ggttttaacg
     7741 gagttgaatc ctgggaaaaa aactcagaag agagcttacg gatagaaaca aagaaggggg
     7801 ctatttctct tcaattattt ctcatcctga atctaaagaa gagagtgccg gaaacagcct
     7861 gggagcttca gaagggatta aaagagatgc tggagaaaaa aactggtttg cgagtggatc
     7921 ggattgatat ctatgttcag ggctttgccg ttcatcaaaa aaatagcacg gttttagcta
     7981 tggagtaaaa tgttgcgaag gaaagcgcga gtagctgctt tacaaatttt atttcagatg
     8041 gatatgagag ataaaaaact ggaggaagta cttccttatt ttaaagttcc taaggggtgg
     8101 gacgaagata ccctttcctt ttttttccgt ttagtgcagg gggtagagac taaaaaaaag
     8161 gagattgatg aaatactttc tcaggcttca gaagagtggc ctctttatcg gatggctaac
     8221 gtagatcgca acatcttacg tttagctgta tttgaaatac tttttcttcc cgaagtgccg
     8281 gtaagcgtgg ctatcaatga ggcggtggaa ttgggaaaga agtatggaac agaggagtca
     8341 ggaagattca ttaatggaat tttagggcaa ttagttagaa gcttagaaga aaggggaaac
     8401 tatcttccta aaagagaaag aacatgaaaa tagaagattt tctccaagaa aatgctcact
     8461 tagtagacga atatctggaa cgtaactttg ctcccctgag cgctacctct tctttagtat
     8521 ttcctatcct ttatggggca aaagggggga agaaaattcg tgcttctctt accctggcta
     8581 cgggtttcgg ttatggagta gaaaaagaac atttactacc tgtagccggt gctatagaga
     8641 tgatacatgc tttttctctg gtgcatgatg accttccttg tatggacaat gatgattacc
     8701 gtcgagggaa acccagtatg catcgagctt ttggagaggc catggctgtt ttaacaggag
     8761 atgctctttt gatagagggg ttacggctca tagtgggaaa tgaagaattg gctaaacttt
     8821 tcggtaaaga aaaagtactg gatatgacaa atgttatttt aaaagctctg ggcatagagg
     8881 gaatggtggg aggacaggta ttagacttag aaggggaggg aagagaagta agcgaggaaa
     8941 aagtttggga gatatatcgc aaaaagaccg gttcccttct taccgcttct attctatgcg
     9001 gagcaatagc tggttccgct ccttcccagg aaaaagaaat tttggaaaac tttggcatgc
     9061 ttttggggga atgttttcaa attaaagacg atatattaga tgtaactcaa gaaaaagagg
     9121 ttttaggtaa aactccaggt aaagataaaa tacaggacaa agctacttta gtgaaagtta
     9181 aaggggtgga agagagcgaa aaaataatga aagataaatg ggaagaagct atctgtcttc
     9241 tttcttctat tcctcggcct ttccccttcc ttttggaaat agccaggttt gtaatagaga
     9301 gaaaatacta agaatgaaaa aaggaaaaat tcgtctggat gagctcttag tagaaagtgg
     9361 cttatgtgac agcagagaaa aagctcaaag aataatcgga agtggaaagg taaaagtaga
     9421 aggtttaagc caacatctta agccctctac tttggtagaa aagggcgttt ctatccagat
     9481 agaggagcct cctcgatttg ttagtcgagg aggagaaaaa ttagaaaaag cgataaatga
     9541 attcggcatt aatcctcaag atttggtatt tgccgatatt ggtgcttcta ccggtgggtt
     9601 taccgattgt cttatccaga gaggggcatc aaaagtttac gctattgatg tggggcatgg
     9661 ccagttgcac gaaaaacttc gccatcatcc ccaagtagtg gttaaagaga aagttaatgc
     9721 tcgttatctt gacttgagtt ttttaggaga gagagtagat ggtgttacca tagatgtctc
     9781 tttcatttct ctccgtctca ttttctctcc cgccacttcc attctcaagg ataatggcat
     9841 attaattgct ttgatcaaac ctcaatttga agctggccgt agagaggtta agagaggatt
     9901 gataaaagat aaaaaagtgc atgagcgagt tttagaagaa attttgactc aagctcaaga
     9961 agcgggactt tcttttcaag gtttaacttt ttcccctctt cggggggcaa aaggtaacat
    10021 agaattttta ggatactgga aaaaggagag gtcagattta acaaatgaag gcttaagaag
    10081 tataataagt cgagtggtgg aagaagctca ccaaagtaag agaaaatagt aatatgaatt
    10141 ttaatccctg ggtatgaggg ataatatttt ccttcgcttc ggcagtagtg gttactatat
    10201 ctgtttcatc agcttggata agacaccgaa gaaaaagaat tatctctctc gagctcaggt
    10261 ggcagaaaga gaagcagctt ctcttttaga aaatagtggt tttaaattag taggggagca
    10321 accagaaaga gaatttttta ttcgggtaaa tggtaagaaa attccttgta agatacgagc
    10381 agattatctt gtgcgtcgag gaagaagaga tatatatatg tggctgaagt aaaaaccggt
    10441 gaatatttgc gttttgatca ttctcgggtg aggaaacagt tgttagagtg ttatctggtt
    10501 taccacccta aagcgattat actggtgaat ggagaaagta atgagttata ccaggtagct
    10561 tttcctttta gtagtgcttc tacctggaga tgagtggtaa ttttcagtag cttattttta
    10621 ttttgggttt aataattgga cgatggataa agttttaagt acagcacata ttttcattaa
    10681 aaagaaaaat cagaaagtga gagacaaggc ccgagagctg gacaattttc tccagtctag
    10741 agggataaat acccttattg tggaaggaga aacccacata gaagaaaagc cagaagtggt
    10801 attcgtttta ggaggagatg gtactttcct ttctgcgagc tggctttatt ctcctttaga
    10861 gattcctctt ttaggtattg atatgggagg attggggttt ttagccgagg taagcgtgga
    10921 aaatatgttt caagcagcag aagaggtgat aaaaggaaaa tttgaggtag aagagcggat
    10981 gatggctcaa tgtcgcattg tctatgggga tggaagttct aataccgatt ttgctcttaa
    11041 tgatgtggta atctatagag gaccttttgc tcagatgatc cgtctttcca cttacattga
    11101 tggagaattt ttggcctcct ttcccgctga tggattaatt gtagctaccc ctaccggctc
    11161 cagtgcttat tctctttctg ccggtgggcc aatacttcat cctattctcg aacttttggt
    11221 ggtaactcct atttgcgctc atactctata tgcccgttca atagtggtag ccccttcttc
    11281 tactattgaa atagttctgg aatcagaaaa ggagggaacc atggtaaccc tggatgggca
    11341 acgaggttat tctatacaaa aaggagaaca tgtcactgtc aataaatctc ccttttcggt
    11401 gcgtttaatt catgtggaac ccaaaagacc gttttattct ctattacgca gtaagttatc
    11461 ctggggaatg gatattcgaa agccagtgga ggaatgttaa aaactctaag aatcgaaaat
    11521 tttgttatcg tcgattatca agaaatagac tttgcacccg gttttaatgt ggttaccggg
    11581 gaaactggag cgggtaaatc tcttctgatc aatgcttttt cttttcttct aggagctcgt
    11641 cctcgagaag agtggataag aagagaaagt gattttgccc gggtggaagg atggtttgac
    11701 atttcttttt ctcctttttt acaggaaaga ttaagagagg aaggatggaa cgaggaaata
    11761 aagggggaga tggttctttc ccgagaagta aatagagagg gaagaagcaa agctcgtatc
    11821 aacggtagaa gcgtcacctt gactacctta aaagaggtgg cttctcatct tgtggatatt
    11881 tacggacaag gggaaaacaa tcgctttctc actcccgaga acaaattagc tattttagat
    11941 tcttttttgg atgaagaggg aaaacttggt cgggaaaatt acctgcatct tagagaggaa
    12001 agaaatgaaa taaaaaagga aatccagaaa ctgaaaaggg ggcaggaagt agaattagaa
    12061 gaaataaaag aaatgttaga agaaatggaa agaatgggtt tgactcccga gaaaattgaa
    12121 gaagtagaag aacaatttca aaggcttgtt aactctcaga ggtaccgaga agtagtggag
    12181 gaggttatat ctcttctttc tggaagtgaa gaaaaagaag gatttttatc ttccttgaga
    12241 aggataagaa ggagtttaga agggaaaaat gaattagctc tttttcctcc ttctgtggaa
    12301 gaaaccctta aaaatgtaga gttagagtta caagaggtgc tctatcaagt agaaaaagtc
    12361 aaggactctt tcgatttacc cccttcaaaa gtggaagaat tggaaaaaga aatagcagaa
    12421 atcgaaaggt ggaagaggaa atatcattgt ctaaccacta aagatttaat ccattttatt
    12481 ggggaaaaaa aagcaaagct ttctcaacta catcaggagc tggaggaatt agaagaatgg
    12541 gagagggagt tagaaattaa agagaaaaac ttaatggaag cacgggagaa gcttttccgt
    12601 catcgagaaa aagcagctaa aatattcaaa gaaaaattag agaaagaatt aaaagaactt
    12661 tcctttccca aagtggcttt ggagatagaa ttggaaaaga aagaggaaga cgacgaagtc
    12721 tccttcctta tctctttaaa tccaggggaa cctcttttcc cggtggagcg ggtagcttct
    12781 ggtggagaac tatcccgaat aattttagct ataaaatctc tgaccagaga ggtgagagaa
    12841 attcccattt tagtttttga tgaaattgac cagggagtag gaggccagac tgctttctgg
    12901 gtaggagata aactaagaaa aattgccatc gggcatcaag ttatttccat cactcattta
    12961 cctcaaattg cctgttttgc ccatcatcac cttaaagtgg aaaagcgagg agatgaggaa
    13021 agcacctggg tggaagtttt ctctctttcc gcagaagaaa gagttaaaga attggctcgg
    13081 atgatgggag acgaaggtcg ggatgattca gctcttcaat acgctgaaac tttgatgaaa
    13141 agagctcaga atgcaaatac ttaaaaacag cagtttttta tctcaagaga tggaagataa
    13201 agaaatattt gttttggccg gtcctaatgg agtagggaaa acggaaatag ctctgaatct
    13261 aacttctttt tttcgctctt tgggtctgga tagttatctc ttagatctgg attttaccaa
    13321 agctgatttt actgctcgaa gtgaacgatt tttgagccag gtttctattt tacccggggg
    13381 aaaaggaaaa tacgctgata ctcctctgtg ggataaagaa attcctgaag ttctttccca
    13441 aataaataaa acccatcgcc tcgttgtaga cttaggggga gacctgaaag gtatgaaatt
    13501 gtggcggagt ttgcaatctt tctggaagaa taaacaagtt cacttaacac tggtgaccaa
    13561 cttctgtcgt cctttttcgg aagaagagtt ttactttgat tttatggaaa gggctaacac
    13621 tactttaaat cttgagtttc actctgttat ttccaatact caccttatag aatacactga
    13681 tggagaaatc ttaaaaatgg ggtggcaaaa ggctggagat ttttcacaaa aagagaatct
    13741 acccattttt tttgtaagta tctgggaaga tctgatagaa aatttatcct ggaatatttt
    13801 tgatagtgca gtggttggca ttcaaagatt tttgcatcta ccatggatga aataaaggag
    13861 tagatagaaa tggctaaagc aaaagttatt atcaatcgag agttttgtaa aggttgcagt
    13921 ttgtgtgtta atatctgtcc taaaaaagta ttaactatcg agaataattt taattctaaa
    13981 ggttactatc cggctacaat ggttgatgaa gagaagtgta cagcctgtgc tttttgcgcc
    14041 caggtttgtc cggatgtggc catatcaatt tatagggagt aaaaaatgga aagaatttta
    14101 atgaaaggta atgaggcatt agcggaagca gctattcaag ctggttgccg attttatttt
    14161 ggttatccca ttactcctca aaccgagata agtgaatatt tgtcccggcg tcttccggag
    14221 gtgggagggg ttttccttca agcggaaagc gagatagcag ccataaacat ggtttatgga
    14281 gcagcgtcta ccggggtgcg ggtaatgact tcttcttccg ggccaggaat tagtttgaaa
    14341 caggaaggaa tctcttacct ggcttctgca gaactaccct gcgtgatagt aaatatgagc
    14401 cgaggaggtc cgggtttagg taacatccag cctgctcaga gcgattattt tcaagctaca
    14461 agaggtgggg gtcatggaga ttaccgcaca ttagtgcttg ctccttcttc ggtacaagaa
    14521 ctggtaagct taactcatcg ggcattttac ctggctgata aatatcgcaa cccggtaaaa
    14581 attctgggag acggtatgct gggacaaatg atggagccag taacctggga agcagttgag
    14641 ctaccttctc ttcctcccaa aaattgggca ataaaaggga aaaatgaggg agaacgaaag
    14701 tttatcctat gtatggaatt agaccctcga gaattagaaa attttaaccg caaactggaa
    14761 aggaaatacc agcagatggc agaagaagat agtgcctggg aaatatttgg agaagaagac
    14821 aaccctgacc tgctccttgt aggttttggg acagtagcgc gcatcttaaa aagtgtggtg
    14881 agagagggga aaaaagaagg atggcgtctt tccctcattc gccccatcac tctttttccc
    14941 tttccttata atgaagtaca ggaaatggcg cagaaagcgg gtaaagttct cgttgtagaa
    15001 atgaatttgg gccagatggt agaggatgtg aaattgggag tggaagggaa agccccagtg
    15061 catttttatg gtagagtagg gggtatgata cctacagtgg atgaaattct ccagaaaatt
    15121 gctgatattt tgagaggaga gggtcgaaaa tgagaacgat ctttgagaga cctaaaactc
    15181 tgattaataa accttttacc tattgtccag gttgtcatca tggcctggct cagagactca
    15241 tagctgaggt tatagatgag cttgatattg caggaaaaac aataggagta gggccagtag
    15301 gttgctcagt gtatatttac gaattctttg accttgattt tgttatgggg gctcacggtc
    15361 gagctccagc agtggctact ggaataaaaa ggtctcttcc tgaccggata gttttttcct
    15421 atcaaggaga tggagacata gccgctattg gcacagcaga aatagtgcac tgtgccaacc
    15481 ggggagaact tattacctct ttttttataa acaatgcagt ttttggtatg acaggaggac
    15541 agatggctcc cactacctta cccggacaga aaaccaccac ttctccttta ggaagaaaca
    15601 cagaagtgca gggatttccc ttcaagttag gagaaatgtt agctcgggct gagggaagcg
    15661 cttacatcgc tcgggtagct cttactaacc ctcggttaat tggtcaggct aagcggtgta
    15721 tcaaaaaagc ttttctcact caaatagaaa agaaaggatt tgcactggtg gaaattcttt
    15781 ctccctgtcc caccaattgg ggtatgactc ctccggaggc agtgcagtgg gtagaggaga
    15841 aaatgatacc ttattttccc ttaggagaaa taaaagtaaa ggaggatagc tctcatgcat
    15901 catgaaatat taattggagg atttggaggg cagggaatcc tctttttggg aagagtgctg
    15961 gctaccgctg gaatgataga aggatatgaa gtaagctggt ttccttccta tggtccggaa
    16021 atgcgaggag ggacagccag ttgtacggta ataatttctg aagaggaaat aggagctaca
    16081 gtgactgacc atcctgatat ttgtatagta atgaacgaac cttcttttaa tcgcttttct
    16141 cccttggtta aggaaggtgg acttttagta gttaactctt ctttaattcg ggaaaattgc
    16201 tctcgtcaag atatcgatat tttagaggtc ccagccaatg aactggcagg aaaagaggtg
    16261 ggagttgctg aaaccactaa catggtaata ctgggagcac tgatagctcg caagccggta
    16321 gtgagttttt ctgcagcaga gaaagctctg gaaaaaattt taaccgggaa aaaggcaaat
    16381 ctcttatcgg tcaattttaa agctttagaa gtgggaaaga gctttgaaaa gaaaggaggg
    16441 gtagtaagtg ttagtaaaag atcggatgaa tagggaggta gtgactgttc ctccttctac
    16501 tactattata gaagctcaaa aaatcatgaa ggacaaaaat attcgtcgtt taccagtggt
    16561 ggaaaaagag gagttggtag gtattgttac ctataatgac cttttggagg cttccccttc
    16621 taaagccact gctttaagtc gatttgagat tacctatctt ttaagcaaaa tgagtgtggc
    16681 agaaatcatg accaggaaag tggtttacgt aaaacctgat actcctattg aagaagcagc
    16741 tctaattatg aataaaaacc aaattggcgg tttaccggta gtagaagatg gtcgagtagt
    16801 ggggataatt actgagtcgg atatttttga aattttagta gagactctgg gagtcaggga
    16861 agggggaact cgcttgactt tagagctccc tcagagaccg ggagttctcc atgaagttac
    16921 aggagtaatt agaaagtacg aagttaatat tctcagttta gctacttttt accagcaaca
    16981 gaaatcagat taccgttatg tggttatgcg attagctacc cgggaaccag aaaaaatcat
    17041 tggagagtta aaccaaaatc cagacgtcaa tgtgtaccac gtatgggtga ctccagagga
    17101 ggaataatat tgttttttcc ttacttagaa aatgaagatg gcttttcttt acctcaggaa
    17161 ataagcaaag aagtgctctt agaaaccgga atttttaagg tggttaaaaa cacttaccgg
    17221 gaaaaaatgg aaaggatttt tattgttcat cctggagcag tagctataag tgccaccact
    17281 caggatgaca agcttctttt aattaagcaa tttcgtgctc cagttaagga gtggttatgg
    17341 gaaatccctg cgggaacttt agaaatagat gaagaaccac tcttttgtgc tcaacgggag
    17401 ctggaagagg aaactggttt tgtcagtaat aactggcaac atctatttcc cctggtttta
    17461 gctcccggtt acagcagtga gataatccat ttctttaggg caaaagatgc ctcaccagtt
    17521 gttgaggccc gagccgggga tgaagatgaa ttgatttatt ttcgagaggt aagcagaaaa
    17581 gaggctcaag aaatgttaga ccgacaagaa ataaaagatg ctaagacgag aattggtatt
    17641 tatgagtgga tgaaagaaaa ggaatgagag tccttattgc tcttcttgat ggggtgggga
    17701 taggagaatt gcccgatgct tccttttatg gtgacgaagg aagtcatact ctaagaaata
    17761 ctgctcaagc agtaggagga ctttatctcc ctgctctgga aaaattaggt ttaggatgga
    17821 tagatgatat tccgggggtg aaaaaaatag aaaatcccct ggcttttaca gggaaaatga
    17881 aagagaaatc accgggtaaa gatactacca ctggtcactg ggaaatagcc ggtataatat
    17941 tggaaaaacc ctttccggtg tatccccgag gttttcctcc ctcggttata gaaaagctcg
    18001 agagagcctg gggtagaaaa atactgggca ataaacctgc ttcaggaacg gcgattattg
    18061 aagaattagg agaagagcac ttaaaaactg gttaccccat tgtttatact tcggctgata
    18121 gtgttttgca ggttgcagct cacgaggaag ttattcctct ttctgagctt tataaaatgt
    18181 gtcaaaaggc aagagaaata atgcagggag aacatgctgt agctcgagta attgctcggc
    18241 cttttgtggg gcgtcccggt caatttaaaa gaacagctaa tcggcgggat ttctctcttc
    18301 ctcctcctca agatacaata ctcgatgtcc ttgcccgtta tggtaagaaa aacattggag
    18361 taggtaagat ccaggatatt tttgctggaa gaggattaag tgagaatata aagatagcta
    18421 acaatgagga ggggtttcga gttttacgag aattgcagaa gagcagaaaa ggagacctgg
    18481 tgtgggttaa ttttaacgat tttgacacta tttttggtca tcgcaataat ccccgggggt
    18541 ttgctcaagc tttagaagac tgggataaag agttaagttc tttcctctcc tttttgcgag
    18601 aagatgagtt cctgtttata actgccgacc acggatgtga tcctactact cccagtactg
    18661 accactcccg agaatatgtt cccctgttag tttttaaccc ctgttattct cagggcgtgt
    18721 ctttaggaat aagagaaact tttgctgacg ttgctgccac tatagctcag atgatggagg
    18781 tggagtggaa tggtccggga acaagctttg ctttccagat attatccgga aaaaactctc
    18841 cttaagtttc aagagatcat ctcctctttt tcaaaaaaac cccgattggc tatagtagct
    18901 ggttctggtt ggggagaaat agtggaggga gagttaatca atgaactttc ctattcttct
    18961 ttaggaattc ctttttttac cgaggtagaa ggacatgctg gtaaactccg tttattaaag
    19021 aaaaacaatc ttacctttct tctttttagc ggtcgacttc atctctatca gggctactcg
    19081 ttttttgagg caactcttcc cgttcttttt tctcgcctgg ctggagtaga agctattatt
    19141 ttaaccaatt cagcaggaag cctttctttt tctctccctc ccggtagcgt ggttcttatt
    19201 gatgaccaag tggaccttac tttttatccc gacccaattt tgttttctcc tccctttttt
    19261 gacaaagaaa ttcaagaaat ttttaaaaaa gtagggaaag aagaaggtat tactcttaac
    19321 cagggagtat atgtaggggt tttaggtcct tcttttgaaa ccccggctga agttcggttc
    19381 ttatcccgct ttggtgatat ggtgggcatg tctacagtga aagaagcaaa attggcttct
    19441 cttttaggtc ttagaatagg gggaatttct ttagttacca actggggagc aggtatgagc
    19501 agttcttttc ttcaccacca ggaagttcta cagaccgctg cttctcttca acctcttctc
    19561 catagattat tttcttcttt tttagaagaa tggggtaaaa atgcaaatcg tagttagtca
    19621 cgaaggaaca gatttcgatg ccttagcttc catgtttgcg gttaccaagc tctttcccgg
    19681 aaccaagatg gttatctggg gaacagctaa tcgcaacgtt cgtcatttcc tctctctcta
    19741 tggtaatttt tttccaattc tcaaagaaaa agaagtggac tgggaaaaag taaataaaat
    19801 atacgtagtt gatactactt attgggagcg cttgagtaaa gcaggagaac tgatcaggga
    19861 tggtaaagta gaagtagtct tttttgacca tcattctgaa ggagaaaatt ctgaaggaga
    19921 aaaaaaggaa ggggtcgtca taattaaaga atatggtgcc tgcactacta tcttggtgga
    19981 aaaaataatg gaacagaaaa tagctctttc tccacttgaa gctactcttc tggctttggg
    20041 catttatgaa gatactggtt cttttacttt ttccaccact cgagctgaag atttatccgc
    20101 tgcttctttt ctcctccgac agggtgctaa agtgggcttt gtgaatcgtt ttactggcat
    20161 tcaacttact ccggaacaga gggaaactct taaaaagatg attaatttgc tccacgtaga
    20221 ggaaatagaa ggaattcccg ttcatttttg ccggatgaaa ttaactgagt atgtagaagg
    20281 tatttcttta ctggtccata aattaatgga cttagaagag attgaactgc tttttgtctt
    20341 gatggagaca gaaaataaag tttatgtcat tgctcgtagt cgtttagaag aagtggactt
    20401 agcgaaaatc ctgggaaaga tgggaggagg aggccatcat agtgccgctt ctcttactgt
    20461 caaaggaaaa aaaatagagg aagtagaaga aatgattcga gaagctctat cagcctctct
    20521 ttcttttacc cgggcaaagg aagttatgtc ctgtccggta aaagtaattt ctccttctac
    20581 taccattggt gaggctcttc taattctcat gcggtttggc ttttctggat taccgatagt
    20641 ggatgaaaat ggaaaattag tgggtatgat tgctcgacga gatgtggaaa aggcctttcg
    20701 tcatggttta caaaacgccc cggtaaagag ctttatgtct tcccagcttg ttactgtgga
    20761 tccagaagat tccatattta aagttcgtaa tttgatggta gaaaaagata ttggaagaat
    20821 accggtggta agaggagata ctcttttagg aatcataacc cgaagcgatc ttctacgagt
    20881 tttgcatcag cgggaaaatt cggtggtgca agagcttact cgtcttaacc tggctgaaaa
    20941 gctatatctc catttctccc ctctcgattt aaagctttta cactttattg gtgatgaatc
    21001 taaaagagcg ggagcaaaag catatttagt agggggagta gttagagata taattctggg
    21061 gtttcctggc tatgatttag atatagtagt ggagggagat gctatagaat tagctcaaac
    21121 catatcccga aagttagagg gtaaagtagt ttcttatcct cctttcggta cggctatttt
    21181 atttctgaaa gatggaaaaa gagtggattt tgcttctgct cgttcagaat attatgactc
    21241 tcctggagct cctcctcatg tagaatacag taacttgcgg cgagacctat ttcgccgtga
    21301 ttttaccatc aatgccatgg ctattagtat cgggacagat gattggggag atttattcga
    21361 cttctttggg ggattaaaag atttagaaaa taaaactttg cgagtgcttc atcctctcag
    21421 ttttgtcgag gatcccgccc gtgctatccg tgctgttcgc tttgagcaga agtatggctt
    21481 cgaaatagaa ccctttacct tgagtttact caaacaaacg gtgagagaag ggttattgaa
    21541 taaaattaaa ccggatcggt taaaggaaga aattaatctc attttacgtt tacctgactt
    21601 ttatcgcttg ttagagcgct tatatcaatt ggatatgttt ccctatatct ttccgggaag
    21661 cacctggaga agagagtttg aacccgttta tcaaagattg gtgaaactct tagaccggtt
    21721 ttcttttcta tctttggatt actttctggc taaactttct cctttatggg gagaaatgga
    21781 tcccgcttat ttaggagaac tgaaagaaag gttagccctt tctaaagctt ctatccgcaa
    21841 aattagtact tacttagata aaaaagagga attagaagaa aaacttcctg atgagacgtt
    21901 acctgattct caagcttatc tccttctacg ctccctgggg gatgaatttt tactcctcct
    21961 tgaggctaaa tggggagagg attccctccc tggaaggaga gtggagcaat atttgaaaga
    22021 atggagaaaa attactccct atcttacagg agaagattta aggagaaaag gaattcccgc
    22081 tgggccgatt tatgctaaaa ttctggaaga gttgaaactg gcaagaattg atggcttagt
    22141 taaaagcagg gaagaagagg aaatattagt agaaaaaatc tggaggaatg aggcgcatga
    22201 aagataaaga aaccattttc agcggaatga gacctactgg taaaatgcat attggtcact
    22261 attttggagc actgaaaaat tgggtagaac tgcaagaaaa ataccattgt atttttggca
    22321 ttactgattg gcatgcttta actacttctt ttgaagatac cgatcaacta gaaaataata
    22381 ttatagaaat ggctgctgat tggttggcag tggggattga tccagaaaag tgtgttttaa
    22441 ttcttcagtc ctgggttcca gaacatgccg agcttcactt attattttct atgattaccc
    22501 cggtgggatg gctagagaga aatccggtcc ttaaagagca acttcaggat atgggtctca
    22561 aagaggtagt agggtatgga cacttaggct acccagtact catggcttct gatattctta
    22621 tttataaagc agtcaaggta ccggtaggtg aagatcaaat cccccacata gaaattactc
    22681 gggaaatagc ccgtcgtttc aactatttat acggggaagt ttttcctgag ccacaagctt
    22741 tgcttactga ggcccctcgc attctaggta tagatgggaa aaagatgagc aaaagtttaa
    22801 ataattatat cgggctctct gaacctccgg aagaagtgaa aaagaaagtt cactccatgt
    22861 ttaccgatcc agaaaaaata cgtttatctg accccggaca tcctgagcga tgcaatgtat
    22921 tttctcttca ccgccttctt actaatcccc gggttttaga gatagaaaaa gattgtcggg
    22981 aagggaagtg gggatgtgtg agttgtaaag aggagttggg cgaattgatg gtggaatttt
    23041 tagaaaaagt ggttcctcac cgggaaaaat atcaacaaga aaaacattta gttaaagaga
    23101 ttctctggga aggaagcaaa aaggctcgcc aattagctcg agctaccttg gaagaagcca
    23161 aagatgctat gaaattgaaa tatgatttta aatcctgaat ctcagtggaa gaaacttctc
    23221 caagaaactc cttttcctca atgcatcgga gtgtgggaag aaattctcac ctttttagaa
    23281 aatcaattag acgaagcatt aggaaaagag actgaagaaa aagaagaggc taatctattg
    23341 caagaaagag aagaaaggtt tcttctcttt actcagcttc tttctagcct ggaggagcga
    23401 gaggaaaaca ctctgaaagc ccctgaaaaa cgatgggagg ctattcaaaa tagagaagtt
    23461 aaagagatag aaaaattaac tctggcagaa cttttatctc tttatgagct ttatttaact
    23521 ccggaaactc ctcctcctga tgtatcactg gactcccagt ttcaaaaatt attgggtgag
    23581 agaaaaatgg taattaaaaa aatggtgaga gaaaaagaat tccgggatat ggatgaattt
    23641 ttctatgatt gcccagatcg aaaagccact attgccactt ttttaatctt gctggattta
    23701 gtttttagaa aggaactgca aatgagaagg ggagaagatg gccgaatata ttttggaaaa
    23761 acctatcctc cggttaaaga acctaattga agctttactt ttttcttctc cccatccggt
    23821 atcctgctcg gaaatggtaa agctaactga agcagacaag agaactgtaa ttcaaactct
    23881 acaagaactt caagatgaat acgaaaaaag agaaggggct cttgctatcc gggaaataaa
    23941 cggaaagtgg cagatgatag tcaaaccaga gtatggtcaa gaactgaaag aaaagatgaa
    24001 ggtaagcagc actaaaacca tttctcgaaa ggctctggaa actctggcca tcatttctct
    24061 ttatcagcct cttactaaaa ctcaaatcga cttaaaaaga ggggtggatt cagcacagac
    24121 tatccgtact ctcttagaaa gagggttgat agctatagca ggacgaagcg acctcccggg
    24181 caagcctttc ttgtataaaa ctactgataa atttttagaa gtatttggaa taagaagtga
    24241 agaggaattg gaaaggctaa agaatttatt ctgggaaaag aaaaatggag agactaaata
    24301 aatttttagc ccgggcagga gtagcctccc gtaggaagtg tgaccaatta ataaaggaag
    24361 gcaaagttac cattaatggc agaataacca ccaatccagc ggagagagta agtggaaaag
    24421 agataataaa agtagaaaat aaagtagtaa agaaagaaga attggtttat atagaactat
    24481 ataaaccgcg agactatctt actactctat atgatccgcg agggcggcga acggttgcgg
    24541 atcttctctc cggggtaact acccgggttt tcccggtggg aagattagat agtgaaagtg
    24601 aaggattatt gcttttcaca aacgatggtg ccttaagcta ttttcttact catccccggt
    24661 ttgaagtgga aaaaaaatac ctggtgtatg tagagggaaa gatagaaaag agagggctct
    24721 ccattttaaa aaaaggaatt acagttaaag atatccctta ccgcattcgg cgaggtgaaa
    24781 tcaaagagat ttattctaac tgtagcgtgg tagaggtcac ccttttggaa ggaaagaagc
    24841 atgaaatccg gataatgttt gattatctgg ggcatcccgt gttaagatta ataaggataa
    24901 gtatgggtcc tttggtgttg gacaaaaata ttcttcccgg ggaatggcgt ttcctctccg
    24961 atgaagaagt ccagcttttg cagaaatatc aggaggaaaa acttggcaga taaatcaggg
    25021 ttaattcagg tatattttgg agaaggaaag ggtaaaacta ccgctgcttt gggaaccacc
    25081 ttgcgagcag caggtcaggg aatgaaggtt cttcttgtac aattctttaa aaacaagttt
    25141 accggggaat tagcatcttt gcaactttta ccgggagtag atgtttaccg gtttggcagt
    25201 ggtgaattca tacgtggaga agtttcccct gaagatcaga gagaattttt tttaggctgg
    25261 catatggtgc gggaagctct ggaaaaaaaa gaatacgatg tggtgatttt agatgagtta
    25321 ccttacgctt tctattatca tcttttaacc tgggaggagt gcagagaagc tttagagcaa
    25381 aagaaccccg aggtggaagt aatcataacg ggaagaaaag taccggaaga gttgatcacc
    25441 attgctgacc tggtttcaga aataaagctg gttaaacatc cctttaataa gggaatacct
    25501 gctcgacgag gatttgaata ttgaaatgga aaagaaagtt agaggaataa gaggagcaac
    25561 cgtagtagaa aaggatgaca aggaggaaat taaaaaagcc gctaaggaac tgtttgacct
    25621 ccttttgaga gaaaataata tctctccttc agaaatagta gcagttttta tcaccgccac
    25681 tcctgattta cattccactt ttcctgctga agctattaga gaaagagatg agtttcgcta
    25741 catccctttt atttgtgctc aggaaatgag agtagagggg gctttggaaa aatgtattcg
    25801 tttcctggtt ttagcctata ccgataaaga ccaaattgat atccatcacc tctatctggg
    25861 aaaagcgtct tcactcaggg aagacctgag ccggagggaa aatctgtgaa agaagtttta
    25921 attcatccct tctctcaaaa agtagaaggg gaaatttgtg cccctcgaga taaatcttta
    25981 agccatagag cagctatgat aggagctctg agtcaaggaa cgacggttat tgatggtttt
    26041 tctttttgtg ctgattgttt ttctactctt tcaggtcttg agctatgggg agtggaaata
    26101 attaaggagg aagaaaaaga gagggttaca attaaaagtg aaggtttagg aaaacttcag
    26161 gaaccggaag atgttattaa tactggaaat tcaggaacta ctatgcggct tttagcgggt
    26221 atagccaccg ggatagaagg acttaccata tttactggag atgagagcct tcgtcgacgc
    26281 ccgatgaaga gaattatgga acctttacgc ttaaccggtg tagaagtggg aggaagaaaa
    26341 ggggattcct ttcctccttt ttttgtgcga ggtaaaaata gggtgaaagc tttttcttat
    26401 cgtcttccta tacccagtgc tcaggtcaag tcggctttaa tttttgctgc tctccgagga
    26461 gaaggaacct cttttattga agaaccctta ccttctcgag accatactga aaatctgctc
    26521 tgttactcag gggttaaaat agaaaaaaga ggtggggtaa ttgaggttaa tcctccttct
    26581 tctctctctg cttcttattt atcacttcct ggcgatttgt cttcggcctc ttttttaatt
    26641 gctctgacca ctcttttgcc ccggtctcgc attttaatta aagatatagg attgaaccct
    26701 actcgagcag gatttttgac ctgccttcgg caaatgggag gaaattttaa gataattggg
    26761 aaaagagaag agtttggtga actccgggga gatatagaag tagaatcttc ttccttacaa
    26821 ggggtaaaaa ttggaaaaga tttagttcct cttttgattg acgaaatacc tataattgca
    26881 gttttagctg ctaaggcacg aggaactact attatctctg gagcagaaga actgagagta
    26941 aaagagactg accgtctcaa agccatagct caaggattga aaacattagg agcggaggta
    27001 gaggaaagag aagacggact tttgattcag ggaccttgtg ttttccaggg aggaagggtt
    27061 aatagctatg gagaccatcg gatagccatg agcttagtag tggcaggaat aacgggagaa
    27121 agagatttag tggtggaaga catagattgt gtaaaaataa gctatcctgg tttcttttct
    27181 gacttagaaa aaattggtta caaggagtgg gagataagag aaagatgaaa aaatatatag
    27241 ccatagacgg gcctgctgga gctggaaaaa gcacagtagc tcaaaaattg gctcaaagtt
    27301 taaaatatct ctatctcgat accggagcgc tgtatcgagc gataactctt tatttttggg
    27361 aaaataatat cccattggaa gattgtaaga ttcttacatc tgtactttct cgaataaaac
    27421 tggaggtttc ctgggaagaa aaatttcgga tatttttaaa tggtagagaa gtaactgaag
    27481 atattcgtca acctgaagtg gatgctctgg tctctccagt agctaaaata aaagaggtaa
    27541 gaaattattt gtgtgttgtg caacgagaaa tcgctctttc tcgtccttct gtcacggaag
    27601 gaagggatat tggaacagta atattacctg atgcagacct gaaaattttc ctcactgctc
    27661 gtcctgaaat ccgagcctat cgccggtgga aggaaaggaa agataagggg aataatgtag
    27721 attataaagt agttttaaag aatgtgctgg agcgagattt tatcgattct acccgggatg
    27781 acgctcctct gaaaaaagcg gaagatgcta tagaagtaga tacctctttt ttgagtgtag
    27841 atgaagtagt agaaaaactt tataaactgg tggaaaacta aaatggaagt atggacagca
    27901 aatagggtag gcttttgccc tggggtaaag agagcttttg atttagccct tcgtgctctt
    27961 tcccgggaaa aaccggtata ttttttagga gaactggttc ataattccca ggcagtcaaa
    28021 atgctggaag aggagggagg gataatagtt actgaccctg aagatatacc tcctttttct
    28081 ttagtggtta ctcgttctca tggattggag aaaagaatta aagaatttct ggagagccgc
    28141 caggtaaaaa ttattgatac cacctgccct cgagtaaaga aggtgcagat tttagctcaa
    28201 gaattagaaa aaaaagggta taatctggtt attttaggta gctctaaaca ccctgaagta
    28261 agagctctat taagctttat ttctcaaaaa gctctggttc tggaaaaaaa agaagaatgg
    28321 ggaaagttga ggaatttttc tcctcaggaa aaattagcag tggtggagca aaccaccttc
    28381 ccccgggaaa taaaagagga atttactcag gaaataaaag aggaatttac tcacctgaat
    28441 ctcgaggttt ttgatacctt gtgtgccgaa acagagatga gacagagaga tttagtagaa
    28501 agtatcaaac ttcataaagt caataaagta gtggtggtgg gaggaaaaaa tagttctaac
    28561 actaaagcct tatttcttac tgcccggaga ttagggatag aatctatctg ggtagaaaga
    28621 gttgaggaac tggaagaagg gtatggggat aaagaggata gaatactagt ggtaagtgga
    28681 acttctactc ctcatttttc ggtggaggat gtggtaaaaa aattaaaagc aagggaagct
    28741 tccttttatg aaagaaaaag tgccgatggt agccatcata gggaaaacaa atgtggggaa
    28801 atccactcta tttaatcatc tttcccggaa gagagtagct acagttgatt ctcaccccgg
    28861 agttactcgg gattttttgg aaaacatagt agatatagag ggaaaaaaaa taaagctggt
    28921 agacaccggg gggttagaaa ttttagtaga ctctcaaaat gagatgcaaa agaaaataga
    28981 agaaaaaacc tgggaggtaa tggagaaagc cgatcttctt ctatgggtat tagacggaag
    29041 ggagaacctt accagcatag actgggaatt ggcttctcgc ctgcgaagaa ttaagagccc
    29101 ggtgataata gtaattaata agagagaagg gatgaccagc gaagccccgc ctgctgagtt
    29161 tttttccctg ggttttgaac ctattataca aatttctgct actcacggag atggtctcag
    29221 acaacttcgc gaaacaattg ctgagctgat agtaggaaac acagaaattt gctttgatag
    29281 acaccgtatt ccggtggcta ttgtggggaa acccaacgta ggcaaatctg cactctataa
    29341 ttctctttta ggaaaagaac gttccattgt aagcgatatt ccgggtacca ctcgagacgc
    29401 gcttttatcc actttagata ctagctttgg ttactacgag ctgatggata ctccaggatc
    29461 aatagctcga tctaaaatta aagagagaat tgatttttat gctgccttga ggactgagga
    29521 aagcttgcag gatgctgtta ttgctctctt ggtgattgat cctattcaag ggattactcg
    29581 ccaggataaa agaattgctc aaaaaataaa ggaagataaa aaaggatgtt tggtgtttat
    29641 taataaaagc gatttggttt taaaccatca ttctgaatgg caagttaaaa aattggtaga
    29701 agaagttcgc cgagagttga gtttcttgaa ttatgcttcc tttctggttg gatctgcttt
    29761 ttctccttct gtctcttcgg atattttatc tgtccttgct ggaattgcca ctcgttatta
    29821 tcagaggata aaaaccagtt tcttgaatga aaaaataaga gaaaaaataa atgaggaagg
    29881 ctggaaagtg gggggcagaa ccttccgcac ctattatatg catcaaaaag atattgctcc
    29941 tccgcgtttt tatttgtcgg tcaatttttc tggcacaaaa agagaagaag aacttctagt
    30001 taaccgcata gaaaaattaa tccgaaagga aggagattgg gagggggtgc ccatatctat
    30061 tgaactctcc cgcgaatgaa aaaattatcc atatttttct tgattatctt caaaaagaga
    30121 gaaggtattc tcctcataca gtagataatt atgggagaac actgcgtcaa ttctctaaat
    30181 ttttagagga gagaaatctc acattagttg aggctaaaac tcaggacata gccgattttt
    30241 tagcctatct caaaactcat catcagctca aaaggatttc tcaagccaat cgtatttctg
    30301 ctcttcgtac tttttatcgc tttcttcgcc gccggggtat ggtcgattgt gaccccaccg
    30361 atggattggg aaaactcaat aaggaaaaga aactgcctaa ttttttgact gtaggagaaa
    30421 tggaattttt actcaattac ttaaaacaaa aagcgcgaga aaagtcggat tttccttcac
    30481 tcaggaactg ggcatttttt gagcttctct actccagcgg tttgagagtt ggtgagataa
    30541 ctgaattaaa aaccaaagat cttgacttaa cgagtaaaat ggtaaaggtg aaaggaaagg
    30601 gaggaaagga aagattggtt cctttcaatg aaagcactca aaaagcttta agaaaatatc
    30661 taaaccatcg cccttttccc cgggaagaat tgttcttgaa ccagagaggc gctcctttaa
    30721 ctcctcgagg agcacgaaaa atcttagaaa caattgtaaa agaggccggg ttaggtggaa
    30781 aaagaatttc tcctcatgtt ttccgtcaca gttttgccac ccacttttta ggagggggag
    30841 cagagctaag gatggtgcag gaagccctgg gtcattccag tctttccacc actcaaattt
    30901 atacccactt gaattgggat aaaatgaaga ggatttatca agaaactcat cctcatgcta
    30961 agaaggtgaa aaatgatgat taagtcaact acggtgattg ctgttttaaa gaatggtaag
    31021 gcagccatgg cctgtgatgg gcaggtaacg atgaatgaaa ccgtactaaa aggtggagca
    31081 cgaaaaatta gacgtattta tcaagataga gtcctggctg gctttgctgg tagtggagca
    31141 gatggactaa ctcttcttga gcgcctggaa gaaaaactta attactatag tggtaatctt
    31201 ccccgtgctg ctattgagct agccaagagc tggagaactg ataaagtttt gagacaatta
    31261 gatgctcttc tcttagctat ggacaaagaa catattttaa tcatttctgg aagaggcgat
    31321 gtggtagaac ctgatgaacc ggtagcagcc attggctcgg gagggaattt tgctctggca
    31381 gctgctaaag cactctggag agaaaccgat aaagaagccg aagatattgc tcgcatttct
    31441 ctacagattg cttccgaaat ttgtatctat actaatagca atatgactgt cgagactatt
    31501 tagctggaga ggagaaaaat gtctttaata gaagagaacc tgacacctga ggaaatagta
    31561 gaagagctaa ataaatatat agtaggccag gatgaggcta aaaaatgtgt ggccatcgcc
    31621 ttgagaaatc gaattcggag aaggatgcta cctcctgatg ttgccgaaga aataactcct
    31681 aaaaacataa tcatgatcgg ccctaccggt gtaggcaaaa ccgaaattgc ccgccgtctt
    31741 gccaaattga ccaatgctcc cttttttaaa gtagaagcta ccaagtttac tgaagtagga
    31801 tatgtgggaa aagatgtgga atccataatt agagatttaa ctgaattagc agtgagaatg
    31861 gaaaaaaatg aacgcataaa gaatcaagaa gaaaaagcta aagaaatagt agaggagctg
    31921 attttagact atcttttggc ttttaatcct tcttctctgg aatatgaaga tactgtaact
    31981 gcagaatctc ctcgggtaga tgtagtttgg cccaagcctc tttcttctac tcgagaaaag
    32041 ctaagagaaa aactgaaaaa gggagaattg gaagaacgtt ttattgaagt tgagatagag
    32101 gaaagctcta tacctttggg aattatagct ggtgggggta tggaaagtct ggggatagat
    32161 tttcaagagc tctttagcgg agttttccct tctcgaaaga aaaagagaag agtaaaggtt
    32221 aaagaagctc gagagatatt attcaaccag gaagtccaga aactaatcga tatggaagaa
    32281 ataagcactg aggcaataaa aagagtagaa gaaacaggca tagttttcat tgacgaaata
    32341 gataaaattg tttctccagg gggaaactat ggcccagatg tttctcgggg aggagtgcag
    32401 agagacttgc tccccatagt ggagggctct actgttctca ctaaatatgg tccagtaaga
    32461 accaatcata ttcttttcat agctgctggg gccttttccc ataccaagcc ttctgacctt
    32521 ttacccgagt tgcaaggaag atttcctatc cgggtagaat tgtcttcact gtcggaagaa
    32581 gacctttatc gtatattaat tgagcccttt aattctttga ctaaacaata taaagcattg
    32641 ctggaaactg aaggagtaag tatagagttt gaggaagatg ctttgcggga aatagcccac
    32701 atttctcagg agctaaacca gagaatggaa aatattggtg ctcgcagatt gcatactgtg
    32761 atggaaagac ttttgcaaga tatctctttt cgggctcctc gtttaaaagg gcaaaagata
    32821 gttataaatc gagattacgt tttgcaagaa cttgctgaca ttttacagga tgaagatgtt
    32881 actcgctata ttctttagtc cttttgatat taagttgaaa aaatttcatc ctttaggtat
    32941 acttacgggc ggtaatttaa taaaaacaca tgtagagtgt ttctggtgct ccggaggagc
    33001 gaaactctac agaggttaaa ccagaaggag gttaaaataa tggtagttac tatgaaacag
    33061 ctcctagaag ctggtgttca ctttggacac cagaccagac gttggaaccc taaaatgaaa
    33121 ccctatattt ttgccgaaag aaacaatatc tatatcatcg atctccaaaa aactgtggag
    33181 ctggcagaga aagcttataa ttttgctcga gacctatctc gagaaggagg aatgtttctc
    33241 tttgtgggta ccaaaaaaca ggctcaagaa gctattgagc aagaggctca gcgttgtggc
    33301 atgttttatg tcaaccagag atggttagga ggaatgctta ctaacttctc cactattcgc
    33361 cagagtatcg agcggttaaa aaagattgaa gaaatggaag agaaaggaat tctggagcga
    33421 cttcccaaaa aagaggttat gcagatcaaa aaaaggaaag ctcgtctgga aaaatatctg
    33481 agcggtattc gagacatgga aaaattgcct gacgctcttt tcgtggttga tcctcgaagg
    33541 gaaagaaatg cggttttaga agcaagaaag atggatatcc ccattatagc tatcgtggat
    33601 actaactgtg atccagacga aattgattat cccattcctg gaaatgatga tgctattcgg
    33661 gctattcgcc tcttttctgc tcttattgcc gatgccgtat tggaaggaaa gaggctggct
    33721 agcgaaggtc aagaaatgat agctacagaa ggaatagcta ttcctcagga agaaatagag
    33781 gaagcagaag tagcggaaga aatagaagaa atagtggtag ataataatga taataaagag
    33841 gaagaggaaa atgtttctca agaggaagat gctttgcaaa tattagaaga actccaggat
    33901 tcagggttag aagaaattga ggataaagaa acagaaaaag aggaggatga tgataattca
    33961 tgagtgctac agctaaagaa gtttttgagc ttcgaaatct gactggagca ggggtgatgg
    34021 actgtaaaaa agcgttagaa gaagcaggag gagatataga aaaagcctgt gatatactcc
    34081 gtcgcaaagg tttagaaact gcagctaaaa agcagggcag agtagcacag gaagggttaa
    34141 ttggggctta tgttcatact gatggtaaaa tcggggtcct ggtagaagta aattgcgaga
    34201 ctgacttcgt tgctcgcact gatgagtttc gctacctggt aaaggagttg accctacagg
    34261 tagctgctca agctcctcgc tgggtggctc cagaaaatgt tccagaagaa ataaaaaaga
    34321 aggaagaaga aatttaccga gagcaactaa aagacagcaa caaaccggaa gaagtcaagc
    34381 aaaaaattgt agaaggaaag atgaaaaagt tttttgagga aaattgtctt ttagagcagc
    34441 cttatattag agaaccagaa aaaaaagtga aagaccttct catggagacc atcgctaaat
    34501 taggagaaaa catagtagtc aggaggttcg tgcgctacaa gctgggcgaa gacaatgagt
    34561 gatcaacaac tatcttcttt gcgatttaag aggatcttga ttaaattatc gggagaggca
    34621 ttaatggggt ctctcccttt tggtattgac actcaggttc taggttatat cactaaggaa
    34681 attgaaaagg ttaaaaatgt gggagcagaa atttgcatag tagtgggagg aggaaatatt
    34741 tttcgaggta gaacggcagg agataagggc atcagtcgag tgactgccga ttatatggga
    34801 atgttagcca cagttattaa tgctctggcc ttgcaagatt ttttggaaaa tgaagggatt
    34861 cccactcgag tacaaaccgc tattgaaatg cgggcagtag ctgagcccta tatcaggaga
    34921 aaagccatcc ggcatttaga aaaaggaagg atagttatat ttgccggtgg taccggtaat
    34981 ccttacttta ctactgatac tgcagctgcc ttgagggcag ctgaggtgcg agcagaagct
    35041 ttgctcaaag ctactaaggt agacggaatt tatgaagctg accccctgaa ggaaaaaaac
    35101 gcttataaat ttgaccgttt agactattta gaggttttaa atcggggact taaagtaatg
    35161 gattctacag ccatttctct ttgtatggaa aacaacattc ctatcatcgt atttaatgtc
    35221 aacgagccag ataaccttgt taaagtagtt actggccata aaataggaac tttagtaggg
    35281 agggagggag aaaaatgaat gaagatttaa aattggtaat caaagatgtg gaaaaaagga
    35341 tgaaacaggc tataaatgta gctcgggagg agctgtctcg tgtcagaacg ggaagagcgt
    35401 ctccagcttt ggtggaagat ctggaaatcg attattatgg aacaattatg aaattgaagc
    35461 aaatagcttc tattaccact cctgatgccc gcactattat aatacaaccc tgggacaaaa
    35521 atgctctttc tctcattgag aaagccatct ggaaatcaga cttaggactt aaccctcaaa
    35581 tagaaggagg ggtgattcgt atagccatac ctcctcttac tgaagagaga agaaaagaga
    35641 tagccaagat agcaaaaaaa tggatagaag aagctaaagt agctatcagg aatcttcgac
    35701 gagaagctaa tgaaaaaatc aaaaaaatgg aaaaaagagg agaaatatct gaggacgaga
    35761 gtgaaagagc gcaagctgaa gtacagaaac ttaccgatga acacataaat gacttggatg
    35821 gtttatggga aaagaaagaa aaagaaataa tgactatata ggtacgatct taaggcacgt
    35881 agccatcatc atggatggta atggaagatg ggcagagaaa cgaggtttgc ctcctctgga
    35941 ggggcataga aagggagtag aagtggtaag gattctggta gaagaaacag caaaagtggg
    36001 catttctttt cttactcttt atgctttctc taccgaaaac tggaaaaggc cagtaaacga
    36061 aattcagggc ttgatgagac tatttgtaga aagcatggac aaatatggca aggagttgga
    36121 ggataacaaa gttagagtaa aatttttggg aaggaaagac ggacttcccg aggacttagt
    36181 tctgcagatg aacgatttag aaaaacacac aatgagaggt gaaaacctga atctcaatct
    36241 agccataaac tatgggggaa gagatgaaat tttgagggca gtgaaaaata tttaccagga
    36301 aaaccattct cttcttgctt ctttgctgga agagaatttc agtgcctacc ttgataccgg
    36361 aaatcaacct gaccctgacc ttttaattcg taccggagga gagagaagaa ttagcaattt
    36421 tcttctctgg caaattgctt ataccgagct gtggttcact gacactttgt ggcctgattt
    36481 caccatagaa gagtatcatc aagcttttca ggactttatg gaacgggaaa ggaaatttgg
    36541 gggtagattt tgaggactat tgctcaatca tcagatttga gaaaaaggac ctggagcgta
    36601 gtctgggctt tacctctctt cctattttta atcctttctt cttcctggac tttaatttta
    36661 gtttttttct tcctttcttt tggttctttg agagaatatg gtcggttagt tcatgaaagg
    36721 gaagaatgga cttcctggat gtgtttttct ctggtttccc ttgtccttct ttacctttct
    36781 ttaaccggca ttgattataa agcaatcctt tttttaggat acattttgtt tctgatgtta
    36841 ataggatggg gacttaaaaa gcctcaacga atggtagagc gagtagcttt ttatcttttg
    36901 gggtttttgt actgctcttt cttccctttt ttctgggtaa aaatgggtat tacttacagc
    36961 cggattagca tagttttttt tgcgcttccc gtatggataa atgatatttt tgcttattta
    37021 gtaggtagaa aatggggtaa acataaaata gttccccaga ttagtccagg aaagagctgg
    37081 gaaggtttag caggagggat aggggcagca accattatgg gattagttct tactactccc
    37141 tctatttctt tttctctatc tcagggactt ttgaccggat ttttgatagc cctggtttct
    37201 ttcttaggag atatctggga gtcagcgctg aaaagaagag caggagtgaa agattctggt
    37261 agttttttgc ccggacatgg tgggatttta gatcgttttg actctttttt cctggttggg
    37321 cctttagttt atcttttgag gttatcttgg ggaggatgat gaagatgaaa gtagccatct
    37381 taggttctac tggttatcta ggagaacaaa tattagaagt tttagaaaaa agaaaggact
    37441 ttgaggttat ccttctatcc ggatattcca aagaagaaag attgagagaa caagcccaag
    37501 aattaaatgt ttcttatgct tttaatcccc ggggtagaaa aagtctctgg gcaaattcat
    37561 ctaccacctt gataaccgat tacaacctct tagaaaaagt tattcttcag gaagtagaag
    37621 gggttttctt cgctgcgagt ggaatagaat tcttagattt gttttggaaa cttattgata
    37681 catcccgtat cttgtgggtt gccagcaagg aaatcatcat tttaaccggg gaattaaaat
    37741 ctcaccttct taaagggcga gaaaatctaa ttcctctgga tagtgaacat aacgctctat
    37801 ggcaaatact aaagggggtt gaaagggaag aagtttcccg ggtctattta actgcttcag
    37861 gaggagcatt ctatgaatgg caggggagtt gggatgatat cactccagaa atggctttgt
    37921 ctcatcccaa ctggaaaatg ggagttaaaa ttaccattga ttctgctaat ctgataaata
    37981 aggggttaga agtaattgaa gcttctgcgc tctttgggtt cagttcagaa gagatagaag
    38041 tagcgataca aagagaaagt tttattcatg ctctggtgga attgaaagac gggttttcct
    38101 tagctttgct ttctcccccg gatatgaggt cggttgttct aaatgctatc tccgggaaaa
    38161 agagagagac gaatcccttt caaagtttag acttctctca aattaagtct ttgagctttg
    38221 actatcctgt gccagaaaaa tttctcggtt tttacttagc caaagaagcg ggtagaagag
    38281 gaaagggtta tccagcgctt ttttgtggag ctgatgaagc tctggtgagc gcctttctct
    38341 cccgagaaat ttgttttgga gatatacctc ttattctgga aaaaatttta gaacaagaag
    38401 tgaagcctcc agaaagctta gaagaagtga aagccatcta ctaccagggc tataagttag
    38461 cccaaatgtt aattttagaa agaaagaata ttcaatgata ataactatat tagcttttat
    38521 ctttgtcatc agtctattaa taatagttca cgaatttgga catttcatca tggctaagtg
    38581 gtgtggagta caagttctga gctttgccat aggctatggt cccaaactgg tatcttttaa
    38641 aagtggagaa accgaatttt ctattcgtct catccctctg ggggggttcg taaaaatggg
    38701 gggaatggag ggaaacgtca ttgagggata tgaagaagtc agcattcctc cagagaaaag
    38761 atttgaccat aaacctctgt ggcaacgaag ccttatcgta gcagctggcc ctattatgaa
    38821 tatagttctt tccatagtct tgctgtttat cgttttcagc gtgacaggaa ttcccgctgg
    38881 taacctaaaa gtacaggaag tggtagcggg aggcccggcg gaccgagcag gaataaaagt
    38941 aggagatgta attttagcag ttaacgggag atctgcttcc aacctggatg aggtagtcag
    39001 tattatttct tcccaccctg gagaagaaat agaattaact gtaaaaaggg gagaggaaga
    39061 aatgatactt aaagttatcc cggagtggaa tgaaacagaa aaacgagcac ttattcaagt
    39121 ggtttttgga atagaaaata aaaggttaaa tcccttctta actttgtttc atagtgctaa
    39181 aacaacaatt agttggtttg ttctaactgc cctgggtttg ctttatgtca tcttgggaaa
    39241 aataccagcc gaaatggcag ggcctctggg aatagcccag atagctggac aagcggctca
    39301 gttaggcttt ttgaaccttc ttatgttttc tgcagtggtc agtgtgtctt tggctctttt
    39361 taatctcttg cctatacccg ttttagatgg agggtacctc ttgctttttc tctacgaaaa
    39421 gatccgaggt aaacctcttg agccagaaaa agtaggttgg atttacttta tcggaataat
    39481 cttgattttt tctttagcca tttttgttac ttatcaggat attatgagga tagtagcggg
    39541 gaaatgagaa gagaaaatac taaagagatc cccctgggta aaataaaagt gggaggcaaa
    39601 aaccccatct gggtcgaagc catggggaga gagaatcccg ctaaaattag agaaagcata
    39661 agagaaataa gagaagctca agaagaaggt tgcgagattt ttcgtttagc tgtgcctgat
    39721 gaagaatctc ttttcggtct ttcccagatt aaaaaacaag tggaccttcc cttaatagcc
    39781 gatgttcatt tcaatttaga attaggaata aaagcagctc agttaggaat agatgcggta
    39841 agagtaaacc cgggaacagc gggtaataag gaaaagctgg aggagttaat aaaagtatta
    39901 catgataaaa acctggctct tcgcatagga gctaatactg gttctcttcc tctccactat
    39961 cgggaaaaaa atcgagtgga agcacttttt gatagtgtag aagaattaat atctattacg
    40021 gagaaaaata actttagaaa tataatcctt tctgccaagt ctactgatat agaggaaacc
    40081 attgctatat atgataaatt gagtcgagcc ttttcttatc ctttacatgt agggctaact
    40141 gaggcaggag agggagaaga agggattgta aaatcttcca tcgctttagg gattatcttg
    40201 cgagaaggaa taggaaataa tattcgggtt tctttaactg ctcctgatcc tgtctcggag
    40261 acccgggtag cctggagtat tttagaagcc ttgaagttga gatctaaagg attggagata
    40321 atttcctgcc ctacctgtgc tcgaagaaaa ggagatgtag tagccttaac ccgagatttc
    40381 aagcggaaag taaaagagag gaaaataaaa atagttggta aagtggctat aatgggttgc
    40441 gaagtgaacg ggccgggaga agcgagagaa gcagattggg gtcttgcttt tgcccctcga
    40501 ggaaaagcgc ttcttttttc ccggggagaa agaataaaaa tggttaaaca agaagaagcc
    40561 gaagaagaac tgatacgttt gattacaaaa gaaatggagg agaaaaaacc ttgagaatgt
    40621 cctcactttt tgttcctact ttaaaagaaa atccccagga agcagaaatt gcgagtcata
    40681 gtttgatgtt acgggcagga atgattagac agctctcttc tggcgtgtac atttttttac
    40741 ctttagggct gcgcaccctt aataaaatta ccaaaatagt taaggaagaa atggatagag
    40801 caggggggca ggaattactt ctacccgctc tacaccctgg agaattgtgg aaagagacag
    40861 gaagatggga gatttatgga ccagaattga tcagactaaa agaccgtaga gatagagact
    40921 tttgtttagg tcctactcat gaagaggtaa tcaccgactt agcccgtcgt gaaattcgtt
    40981 cttatcgcca gctgccactt ttattatatc agattcaaac taaatttcga gacgagatca
    41041 gaccgcgatt tggagtgatg agagcccgag aatttataat gaaagacctg tacagctttg
    41101 atcgggattt tgaggggttg agagttagtt atgaaaagat gtacaatgct tattgtagag
    41161 ttttcagtcg ttgtggttta tcctataaaa ttgttcttgc cgagagtgga gtaatcggtg
    41221 gagaggtatc acacgagttt cttattccgg ctgattctgg agaagaaaaa ttcttggtat
    41281 gcccggcttg cgatttcggc accacccaag aggaggcaaa atgccctaac tgtggagata
    41341 gcttggaaga gaagagggga atagaggtag gtcatatatt tcaactggga accaaataca
    41401 gtgagtctat gaaagcttat tttgtggata aggatgggga agaaaaaccg ttaattatgg
    41461 gttgttatgg tattggtatt ggtaggacca tggctgcagc catcgaagct tgtcatgatg
    41521 aaaagggaat tcagtggcct cttagcatct caccctatga agtagtaata ataccagtaa
    41581 actggagaaa agcagaacaa agaaaagcag cggaagaaat ttaccaggaa ctaaagaaaa
    41641 gagactttga ggtagtgata gatgatcggg agttcagtgc tggttataaa tttagtgaag
    41701 cagatttaat tggttttccc cttcaggtaa tcgtgggaga gaaaatgtcc caagagggaa
    41761 aaatagagat taaagttcgt cagaataacg agagatggga gacaaaaaag gaagaggttt
    41821 taactcttct tttaaaatta aaggaggaat tggctccgaa agtctgagct aacgaaaaag
    41881 tgaatgtaag tataaatgct aaagccacag ccattatagc tgcttataat gaggaaaaaa
    41941 ccattggtcc cattattgat gttttaaaag ctatcccttt cctcaatcag attgtagtgg
    42001 taagtgatgg ctctactgac cacacagtag atgttgcccg aagtagggga gtagaagtgg
    42061 tagagctatt gtataatgtg ggtaagggcg gggctctcta tcaggggctg atgaatacca
    42121 cttctaatgt agttctttta ttagatgctg acttaatagg gcttacccat cagcatgtaa
    42181 tagatttagt taagccggtt atagatgggg aggctgacgt taccatcgga attttcgaag
    42241 atggtcgttt tgcgactgat tttgctcaaa aaattgctcc ttttctttcc ggtcagagag
    42301 ctataagaat cgacatcttg caaaatattc ccgatataga agtttcccgt tatggagtag
    42361 aagtggctat taatcgctat attcggaaaa atggagtaag agtaaagctg gttaagcttc
    42421 ccgggctcac tcaagtaatg aaagaagaaa aattaggatt gattaaggga ttgagagaga
    42481 ggatgaaaat gtactgggat attattaaaa acttttacag gggaggtgat aaataaaagt
    42541 tggaggaaaa aaggggaatt ctggttctcg tggatcaggg aaacaatcgc tctttttctt
    42601 cctgggaagg agaaaaaaga gaaattgaag aagttgcctg gagcgcgggc tggaagattt
    42661 tggcagaagt agaatctcga gtaaaaaaac ctttttataa gtattacttg ggcaagggaa
    42721 aggtagagga aatcaaaagc ttaattgagg ataatccgga ggctaatttt attctgttta
    42781 atgtagaaat aactccttcc caacagagaa atctggaaga aacctggcaa gttcctgtat
    42841 ataccaagaa tgctttgatt catgaaatat tcgctcaaag agctcgcact gctcagggga
    42901 aaattaaagt agaattagct cagctccgtt atcagctcag tcgcttaact ggaaagggta
    42961 gagagctatc ccagacagga ggaggtatag gcacccgagg tccaggagag caaaaaattg
    43021 aggtggaaag aaggaaaatt aaagaccgca ttgcccactt aaatcgagag ttacagaaaa
    43081 tagagagaca gagagatgta caaaaaaatt tgcgtcgagc aagaaaaatt cctaatgttg
    43141 ctttagtagg ctataccaat gctggaaaat ccactttgct aaatgcccta actggttctc
    43201 aggtttatgt agaaaagaaa atgtttgcta ctttggatcc taccacaaga aagcttttta
    43261 tccctggagt gggagagact ctttttaccg atactgtagg atttatccgg caaatgccca
    43321 ccactataaa ggatgctttt cgagctactt tagaagaaat taaagacgct gacctgatcc
    43381 tggaagtaat agatatttcc gatgaagatt actatctcca ttttcaagtt ataaatcaaa
    43441 tcctagaaga gctgggaata aacaattctc ctcgtatttt ggtattcaat aaaatcgatc
    43501 ttcttcagtc acccttggtt ttagaaaggg aaatcgaaga aaattatgtc tatgctttca
    43561 tatcggcaca gaaaacaacg ggattaaaag agctcaaaga aaaaatttct ctttctttac
    43621 aacacgaggt cagacatctg tgcttgcagg ttaatcactc tcagcttcca ctgatacaag
    43681 aggaaatata tcagcagggg tatatagaag aacttaaaac tatgaaagat ggagaagtaa
    43741 tgataaaatg cgtggttcga aaggaagccg ctgctaaaat ttgtgatctt tctgaagttc
    43801 ggttctgaag ttcaggttta atagtttagt tcccataatg tatattatgt aaagttttaa
    43861 ttctacttac ctagataaat tttctgccag tttaaaatcg agtttcttct agatcaataa
    43921 ttcggatagc cctgattatc cattctaaat gatgaaaaga taacctcctt tatctttaac
    43981 ccactgggct acaactatta ctatctcatg aatgtgtttg gtagaataga aatgcagcac
    44041 agcggaagtt aaaaagtaca gcaggaa
//
LOCUS       DAOPSS010000003        31346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_1128_length_31346_cov_9.921894, whole genome shotgun sequence.
ACCESSION   DAOPSS010000003 DAOPSS010000000
VERSION     DAOPSS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 31346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31346
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1128_length_31346_cov_9.921894"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1554
                     /gene="xseA"
                     /locus_tag="PKN31_00480"
     CDS             211..1554
                     /gene="xseA"
                     /locus_tag="PKN31_00480"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOA98433.1"
                     /translation="MENISQPSYLTLKELNQILKDVVENCYFSAPLWVVAEIAEIRLN
                     STTGHCYLELVEKEDDRVVARMKGIIWSSNYPLVVSSFLEATGKNLERGMKVLFLTRL
                     RFHPVHGLSLDIKEVDPSYSLGEMMRKRREILECLEKEGFLTLNRSLPFPLFPQRIAI
                     ISSPTAAGWEDFISHLQNNVYGFTFSCQLFPTLMQGEEAENSLFSSLEEIKREQNYFD
                     IVVIIRGGGSSVDLSCFDSYLLGKAVATFPLPVVVGIGHERDDTVLDYVAHTSLKTPT
                     AVAEFLINQMKMVEEEIEEEMAELKEIIENFIEKERRVLTVLVDQWTRRVGDLLLSSG
                     KELSFCRERLLVHSQRILGEIGENQRAYERQLKNLVEERVSKEWEALKRAEQAIRLLN
                     PENVLRRGYSVTYREGKVVKSFRDLQEGENISTRLMDGWIKSKVEETYGREQSEL"
     gene            1529..1732
                     /gene="xseB"
                     /locus_tag="PKN31_00485"
     CDS             1529..1732
                     /gene="xseB"
                     /locus_tag="PKN31_00485"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOA98434.1"
                     /translation="MEENNLSYSEAFQELKSIVDELENEEIEIDNLTEKVKRASFLYR
                     FLRDRLRATEEEIRKVLEEEEPF"
     gene            complement(1745..2650)
                     /locus_tag="PKN31_00490"
     CDS             complement(1745..2650)
                     /locus_tag="PKN31_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOA98435.1"
                     /translation="MEENHKFSHPHNNEKALSVAIILNFIVSLIEIIGGIWSGSLALL
                     SDALHNLGDTTSLFISLFSLRLSLKEETPQRTYGYRRAEVLGAFVNSLLIFATLGYIF
                     IEAIQRLFSPRVIETHILLEIAIIGLLGNLFSVFLLFPGSRESLNLRSSFWHLLVDTF
                     SSLAVIIGALLIKHWGWFFIDPLLSIGLSILLLRGSWKMFKEVVNILMQGAPPHLNLE
                     KLKKALETLPGVKNIHHLHVWSLNEKEHFAEFHVTTTCSSWEEIDQLREEIQKILFED
                     FRIEHSTIQFENKGCKNQDFIVKEK"
     gene            complement(2702..3097)
                     /locus_tag="PKN31_00495"
     CDS             complement(2702..3097)
                     /locus_tag="PKN31_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOA98436.1"
                     /translation="MTNFSDLFQSADWKEEKHVPVIEGPDTVKKGELTNFTATVGKEI
                     PHPNTTEHHIRWIAVYFLPTGGKFPFQLGHFEFTAHGESTEGPNQGPVYTEPRVSFSF
                     KTNQPGTIFVTSYCNIHGLWKGEKEIKVE"
     gene            complement(3110..3622)
                     /locus_tag="PKN31_00500"
     CDS             complement(3110..3622)
                     /locus_tag="PKN31_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOA98437.1"
                     /translation="MPIKRKMTRENLENAYAGESQAYMRYLIFSQVAEQEGKPNIARL
                     FKAIAYAEEVHATNHYRALGYINNTVKNLDMAIAGEIYEVEEMYPAYNAVAKLQGEKE
                     AEITTHGALEAEKIHVQMYKKAKEAAEDGKDVALGTIQICSVCGYTAEGEAPEKCPIC
                     GASREKFKAF"
     gene            4022..4831
                     /locus_tag="PKN31_00505"
     CDS             4022..4831
                     /locus_tag="PKN31_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98438.1"
                     /translation="MRALRRYVVVGLVIFVAFFLGTLAGDIPISHSKEDMKFVAERVG
                     FTAQSFYTAGSNQVYYQIERNLKALDEIINMIEKAENLDAIIDPMVDKLEEITVSYEH
                     ISDLRPQIEEELRGKIEELQELLKKSKDQIRILEDEKLELEEELKELSLEQDEEIRNI
                     RRRSLEMQVEFKAKEIQIWKEFISLEDKIYETIKNADRTIKKFLLVLSENAKVYRGAL
                     EVVRLQRDIKSLVNFMSLQQFSDELISTWQDLQYLIELLTQEVNKLPAFRE"
     gene            4851..5099
                     /locus_tag="PKN31_00510"
     CDS             4851..5099
                     /locus_tag="PKN31_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98439.1"
                     /translation="MEKGETRRGFRKIGWWIILLVMVIVFFLILGGSKRWEWFSPPRT
                     WSDIAILVQEVEEKTQKLNQILQEALQSVREKESPSEP"
     gene            complement(5100..6137)
                     /locus_tag="PKN31_00515"
     CDS             complement(5100..6137)
                     /locus_tag="PKN31_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOA98440.1"
                     /translation="MDLYLGVDVGSVTTKFVLIDEEKNILASCYLRTSGDPIGALKEG
                     LQYIERHFPSGGEIKGVGSTGSARHLAGIVVGADIVKNEITAHAIAAITRVPEVRTVF
                     EIGGQDSKLIIIRDGVVEDFAMNTVCAAGTGSFLEHQAVRLNVPIEEFGKLALRAEQA
                     VRIAGRCTVFAESDMIHKQQLGFGKPEIVKGLCEALVRNFINNLATGKTIEDPIVFQG
                     GVAANEGIVQAFRDFFQGKKKIVVPPEHGVMGAWGAAILALEYAPEKSTFSGFEVIGR
                     QFESRSFTCEDCPNACEIVILKEENDIKALWGSRCGKWTPVTVRQRWEEQEDKSEEPL
                     QIEEEESLVKG"
     gene            complement(6138..7238)
                     /locus_tag="PKN31_00520"
     CDS             complement(6138..7238)
                     /locus_tag="PKN31_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98441.1"
                     /translation="MKATFPHLLHLDIALGVLYPTLGVEIITPPPITQKTIEIGVKLA
                     PQNACFPLKVTLANFVEGIELGADTIFMAGGVGPCRFGYYGQIQNIILEDLGYNVDFV
                     LLDHPRYGIRPFIKALQTMAGGRVLSSRVIKVASLAWKVLRFTEDLETERRALRAQVK
                     DRQKLDEIVEGALEQLRQVNNEKSFQAIKEETMKKLDNLERKETEDLIKVGIVGEIYV
                     AQETKINFNVEKILGELGAYVHNSVSTVEYVLHTLPFTRSREKLEAWELAKPYLKRFV
                     GGEGIDTVGSAIRYAQWGFDGVVHLYPFTCMPEVVAKSILTAVSRDYDLPTLHLAIDE
                     HSGEAGLVTRVEAFVDVLERRKKLKKKKVEVS"
     gene            complement(7204..8202)
                     /locus_tag="PKN31_00525"
     CDS             complement(7204..8202)
                     /locus_tag="PKN31_00525"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOA98442.1"
                     /translation="MKVGIPRGLLFYRFYALWKTFLEELGAEVVISPPTNKGIIQKGL
                     TYAVEEICFPAKVFLGHVLYLKDRVDLIFIPRIVSFHRREYNCPKILALPDLARNLFN
                     IPEERIIGDEINMREEGMRGWRRGFLNIGKHFTTKESKIMKALNLAEKAHKDYQANLK
                     KGMLPEDLIDHKNPLPLREKKIVLLGHTYLLSDSYINMNITEKVNDLNYSVITPDMVD
                     EEKKRYYLSTIPKPSFWTISNEIIGSTLYHLKERKPEVRGFIHLVSFECGPDSLVGEL
                     IERLLKREKESLPYLRLEIDEHTGEAGLVTRLEAFVDMMEWRYKSLESYVSPFASS"
     gene            complement(8277..8750)
                     /locus_tag="PKN31_00530"
     CDS             complement(8277..8750)
                     /locus_tag="PKN31_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA98443.1"
                     /translation="MTIEKNLSYCQFKELIPDLVSIYQRAYENLPEYRDDSYCKIAAY
                     FSWLWKGDPEGFLVVFEGNKPVGLLGMHANWREGKEYLAEIHELVVDPQFQGRGVGKK
                     LIEEAIQIALSRGRKKIELWVGEKNRKAQEFYLHNGFQEKEQRGIWIRMIEELDL"
     gene            9339..10907
                     /locus_tag="PKN31_00535"
     CDS             9339..10907
                     /locus_tag="PKN31_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOA98444.1"
                     /translation="MGNGYRVGVVDNLDKLFAVLPDHIRILLEERENADDLIEVVMDL
                     GRPPEARFAKGFVILSDRIIEREDLDYVVQRVGMFTRDNRAGIERTLHRISCIRNRLG
                     NIVGLTLRVGRAVYGTIDIIRDVIVSGKNILLLGPPGVGKTTKLREVARVLSDEFQRR
                     VVVVDTSNEIAGDGDIPHPAIGRARRMQVSSPEKQHDVMIEAVENHMPEVIIVDEIGR
                     EEEARACRTIAERGVQLIATAHGNTLKNLLLNPTLSDLVGGIQSVILSDEEAHRRGSQ
                     KAVLERKAIPTFDVVIELRDRDTLGIYHDTAQAVDLLLRGYDPRPEVRKRTKEGTVKV
                     VEEDKRASSFVDAKMAQETEVVPEFFESQLLRVYLFAVSKNYVQRAIAHAKVPLEIVE
                     DINQADILLTLKSRYRNKGSKIREAENRGIPVHVVRSNTYNQIYKFVQDLFGYSDLSS
                     GNGDSGLIEAERAARQVLKVGEAIELSPRNSSVRRLQHKIAERYHLFSKSVGEEPFRR
                     VVIYPRILEENRHE"
     gene            10900..11556
                     /gene="tmk"
                     /locus_tag="PKN31_00540"
     CDS             10900..11556
                     /gene="tmk"
                     /locus_tag="PKN31_00540"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOA98445.1"
                     /translation="MNERPGFLITLEGIEGSGKTTHARRLFSFLKESGIPCLLTREPG
                     GTPMGEKIRDILLNPESGVIDPLAETFLFEASRREHVKEVINPALHEGKVVICVRFTD
                     STLAYQGYGRGVPLSYLCYLNDIATEGVMPDLTVLFDVEPEVGLARSFSHTEEEELRF
                     EKEFLASKEFLFAVRNGYLQLAKESPQRFVVVDTTDYSEEEVFFIIRDTIWQRLRGRG
                     "
     gene            11561..11899
                     /locus_tag="PKN31_00545"
     CDS             11561..11899
                     /locus_tag="PKN31_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOA98446.1"
                     /translation="MSPNLLLICVVRDQDVAKLLDNLRDRNISFTKLASTGGFLREGN
                     TTLLIGIEEERKEEVRKLIRETCSRREEIVESTVPLNEPIGPFVPQRIKVVRGGGVLF
                     EVPILYYERF"
     gene            11934..12932
                     /gene="holB"
                     /locus_tag="PKN31_00550"
     CDS             11934..12932
                     /gene="holB"
                     /locus_tag="PKN31_00550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOA98447.1"
                     /translation="MSWSEICGHERQKEFFERAVLSGRVVHAYLFSGPSRVGKTTFAL
                     ELVKLLNCLNPTEEGSCDRCENCRLIINLTHPDISFLSPSGDYLKIDEIRSFVRWLNF
                     KPLMGKMKVGIIEEAEKLTEEAANCLLKTLEEPPGESMVILITSQRASLLPTIISRCQ
                     VVEFSPLSSSEISNYLVEKKGWKEEEARRASSFSQGSLGKAEEIKEGRSGLEDKFWEL
                     WYKLKSERDSLSLGSWFYDNKEQLPLIMIFLEQYLRDLWVWKVTEGQCSNLLFFEKEK
                     VQEEAKEQEAETWRRLIILVDNLISDISGNLNWDIVLCRFLFLWRGEWNRASSSRN"
     gene            12910..13701
                     /gene="ricT"
                     /locus_tag="PKN31_00555"
     CDS             12910..13701
                     /gene="ricT"
                     /locus_tag="PKN31_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOA98448.1"
                     /translation="MPQVVGIKFENNPKIYHFETRGLDLRSGDKCVVETSIGLEIGIV
                     AKEVFLAPEDNFKPVIRKAQKIDLDQAEANREKEKIALQKAEELVAHYQIPMKILRVH
                     YTLDRGKLTFYFGSEERIDFRQLVKELAAIFQTRIELRQVGVRDEARMMGGCGMCGRE
                     LCCNTFLTNFDPISIKMAKEQNLVLNSAKISGVCGRLMCCLAFEYPLYRRLIHRLPKK
                     GSKIITPWGLAKILEVDIFKDSISLELEDGKKVRITEEEYHRYFL"
     gene            13768..14373
                     /locus_tag="PKN31_00560"
     CDS             13768..14373
                     /locus_tag="PKN31_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOA98449.1"
                     /translation="MDKAEVQVWYQGLTLEEEKLWFEEKVKSCESGLLRFAYHLSGNM
                     DKARDLVQEAFLRAYQYRHSYNANYSFESWMSAILLNVHRQRFKKEKFFKIFHPGRER
                     EDGEETEVWDFLESKGEGPEERTLKREVLSFLQEAIEKLPPQMKEVIVLCDVMNYSYE
                     EASEIIGCPLGTVRSRLHRARKKVRSLVEESYGEDFLSIWG"
     gene            14378..14830
                     /locus_tag="PKN31_00565"
     CDS             14378..14830
                     /locus_tag="PKN31_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOA98450.1"
                     /translation="MNCKEAGKMLSAYLDEELTAEKYRSLKRHLMSCSTCQEELEKLR
                     VIRNLMRDLDSDSNFNWNFKVDWEAVRRQARAMRRREMAFFSLAAALVIIALFFVFSQ
                     EQAQWEIEVMHPEVLVDLHQSLSGNQLDIPLKQETNFIGTLELVSGGR"
     gene            14830..15804
                     /locus_tag="PKN31_00570"
     CDS             14830..15804
                     /locus_tag="PKN31_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98451.1"
                     /translation="MRKSVFWIFVGFSFFLLIFCWGFSSFSVAGAEIVNPEEALSFLK
                     EMVKKSTDRDYWGIWSNRDCRSGREDFYEVIHLKYIGLAWRNLNEGDLVTVKKGNYRY
                     VVDLASGEVKGVYPLYTLPFLPPEENNLELLLENYLLDLQDRKGRVISRHTGKVIRSF
                     SVDEKGSLVSQAFYTSEGKLKEEGSFVYRDYSPDYTQLIKYVEAMKKPSDITIPSLSV
                     SGKKLFQPSLLPPGFHLKQVYLVKNEDRDWYQMVYTDGLQYFSLWQSVYPLTLSGEKR
                     NRKISLKKEDDIVTLMGEKSGFYLALMGNFDLETITQIFNSITWEGGN"
     gene            15807..17216
                     /locus_tag="PKN31_00575"
     CDS             15807..17216
                     /locus_tag="PKN31_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOA98452.1"
                     /translation="MWIKKTKLLSFWTVILLVSLVGGVASAVSAIPETTNIVADIVEK
                     VSPAVVNIDTLKMATYRSPLAPFFSDPFFRRFFEGIVPEERQVPIKGLGTGFVFRSDG
                     YILTNEHVVSGADEIKVTFLDGREFEGKVIGSDTLTDIAVVKIEAQGLPTISLGDSDA
                     ARVGEWIIAIGNPYGLSHTVTVGVLSAKGRPIAAGDSGQEYENFLQTDAAINPGNSGG
                     PLLNLEGEAIGINTAILPYAQGIGFAIPINMAKSILDQLITEGRVIRAWLGVVIQNLT
                     PEMAEKFGLKEAEGALVADISPDSPAEKAGIKRGDVILKVDGIETPDIKTLQQTIRSH
                     RPGDVVDVEIWRDGKVITLKVTLAELEESISVLPVTEKIDLGLEVGEITPDLVDRYAL
                     REKSGVVIVGIKAGGPADDAGLRPGDVILEVNREEIKNLKDWEAAISEIEPGETVLLL
                     ISRAGQTYFVPLKAEEPQS"
     gene            17222..18070
                     /gene="rsmI"
                     /locus_tag="PKN31_00580"
     CDS             17222..18070
                     /gene="rsmI"
                     /locus_tag="PKN31_00580"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOA98453.1"
                     /translation="MEVGKEWGKLYFCPTPLGNLQDITLRTLQVLQEVDFIACEDTRV
                     TLKILNHYHIRKPLFSYHSYNLREVSSRIVKLLKEGKNIALVSDAGMPGIQDPGMELV
                     NILEEEGLSFEVLPGPSALVVAAVYSGFSFSGFTFLGFLPRKKKERRKILEQLLYLPP
                     AIVIYESPHRVVSALEEIEEIAGSERRIVFLRELTKIHEEILRGKLGDILGILSQREK
                     IKGEVVLVIEGEKIEKQIPPEAERLVKSLSRQGISPRQILEVVSDAFSISKNKLKEVL
                     EREKSS"
     gene            18135..19019
                     /gene="pstC"
                     /locus_tag="PKN31_00585"
     CDS             18135..19019
                     /gene="pstC"
                     /locus_tag="PKN31_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOA98454.1"
                     /translation="MILKRKKREGAEIVLFLIAFSSLFFLIGIVVVLFAEGLPVFAHV
                     SLSQFLLGRRWYPTSSPPQFGALPLILGSLLVTAGGLLFAVPLGLFAGIFLAELCPPR
                     GREILKPIVELLADIPSVVYGFFGVLVVAPFIRDFLSLSTGLTAFTASLILGIMAVPS
                     IISVIEDAITSVPRSYKEASLALGATHWETLSQVVLPAASSGVGAAIILGAGRIIGET
                     MAVLMVAGGAPVIPKSFFQPVRTLTATIAVEMGEAPVGSPHYHSLFGLAIILFLFTLV
                     LNILVESLATRGREGRTR"
     gene            19016..19891
                     /gene="pstA"
                     /locus_tag="PKN31_00590"
     CDS             19016..19891
                     /gene="pstA"
                     /locus_tag="PKN31_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOA98455.1"
                     /translation="MIKEESLKKRYLVQNIWFTIFWLSMVLIAAVLGLILYFILSRGI
                     KVINWEFLTAAPRRGMTEGGIFPAIVGTFYLVLGSVVVSLPLGVFSAIYLTEYASPGH
                     PIVRAIRTAIHCLAGVPSVVFGLFGLAVFVKIFGFGVSLLSGALTLGIMSLPVVITSV
                     EEALKTVPLSFREASLALGANKWTTIWRVVLPSSLPGILTGLILAVGRVAGETAPIMF
                     TAAVFYARRLPQSLFDPVMALPYHIYGLMTEGTRPDKQIPLAYGTAVVLILLVVAIDF
                     IAIYLRGRARRARKW"
     gene            19895..20653
                     /gene="pstB"
                     /locus_tag="PKN31_00595"
     CDS             19895..20653
                     /gene="pstB"
                     /locus_tag="PKN31_00595"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOA98456.1"
                     /translation="MEEIKLQIKDLNVFFGEKQVLHQINLNIPANKVTAIIGPSGCGK
                     STLLRSINRMNDFLEDVRVEGEIIFDGSNIYDESVDPVVVRRRIGMVFQVPNPFPKSI
                     FENVAYGLRIQGWKDKKAIEEQVVKSLQAAALWDEVKDRLHSSAFDLSGGQRQRLCIA
                     RAIAVEPEILLMDEPASALDPIATARVEELIRSLIEKYTIVLVTHNMQEAARTSDYTA
                     FLLLGRLIEFGLTNQIFTNPQKKETEDYLTGRFG"
     gene            20727..21437
                     /locus_tag="PKN31_00600"
     CDS             20727..21437
                     /locus_tag="PKN31_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOA98457.1"
                     /translation="MQIFSLITKGGYVMYPIILCSIISLAIFLERWYVLRHSRERVDR
                     YLRSVKRAVAQRDWESVVDISKKNQSPPSRIILAGVRKYFQGLTGEAREAMEDVAMQE
                     LPVYEKRLSTLSVVASISPLLGLLGTVTGMIRTFDVISVVGVGKPAEMAGGISEALIT
                     TAAGLIVAIPTVLAHHYLSNLADKIISDIDRACTEVVEALEESANSHNTLPLGSENLR
                     EGEEENVPISVSEEESYS"
     gene            21397..25047
                     /locus_tag="PKN31_00605"
     CDS             21397..25047
                     /locus_tag="PKN31_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA98458.1"
                     /translation="MFRFRSQKKKAIPSIDRIKIFLLFLLLFVSFFSGIVEAQERVKV
                     AVLPLVGKDREDAYWGYFLRDIIKMGLEGEGGVEICDSLLADEIVREARISWSDVLMP
                     STAKIIGERIGCSYLLTGSFRYRLMAGRERILVSARIFEIESGDYLDIPSEIFDIDRL
                     PDLVEYIVGESLYFMGLDYRAPIYLPPFAIDNLLPLYRGVQKMDEALATYGEAQYPDK
                     PLWKEAFSLAEETIEKEPEYLDAYSYLAYMYRKTSWWAKEVETWDLYLQKLEERGEEV
                     DNRQISQTYLRLAYSYFYQKNLNLARESLQKAVEISPQWAEPYLLWARIEYEDGNIEE
                     AERLYSQAFELDPSLKEAEYFAQLAGKAKVYGKSAYEAYVAGYQSFAAGNLEKAEQYL
                     REAIHLNPEMKEAYYWLGRTLYDAGKSKEAEKAWEKVLEIDPFHSQARRFLDKTQQEV
                     KYGREALNYFRQGYELYEKGLYEEAAGLFRQALKVNPVFPDAHDYLARCYYRLGKTEE
                     YVREREESAKLLEDAEEKAWQYYQVGYELFSWGEKEKALQTLEKAIKEDPSLADAHLL
                     LGELYGEKGNWAKSGEHYVQALEGLEGESRGLALWGAAVASFHLENWEESLSLLNELV
                     VNYPYGEFIEEAEVMRMEALVREGKYEEAQRSFQQFLVRFPQSPFLERAQFWYASSFY
                     QAKDWGNAQKFLEEFLRLYPQSSFRSSTIEMLGYTYRNLGKEGEAQKYFSQLEGEEGD
                     FLTADAYYREKDWKKAISSFEDYLSRYPQGKFSLEARFKLASSYLETGNPDKAQELIR
                     GWEGELQNKFKKDFLPFSIKLHAQKEEWREVKEEILRLEKESGKLEEEYYLLLALAYT
                     NLGEEEEAKNVLARAGKDPEEILASPEKEEAQEILDLMERGDYHSALSRLKVLREEES
                     PLISKSMIDFLEGKCCYQLGDFSQAFNLLSRALASPEESFKEEALFYLFNSAYKLERW
                     DKVVEAYSQMKNKDDSSVRFRAALAYYYSGNYEESIPILRSLQDDPDLSSSVISLLVE
                     ELFLLKDYNECGKEAEKFLITFPQDPQRERVLYLASWAFYYSDDLEKAEESIKSYQTE
                     FPGGKYREELSSLLADLYLFQGELDEAITILKEMEWSSDPQRKLYTWYRLGNAYLGKE
                     DFARAFPYFERLFQAGQSEYQTAAGYWWGVCLEYLDRLEEAKEVYRAVIETGQEDQWV
                     SRAQERWDVLTR"
     gene            25073..25783
                     /locus_tag="PKN31_00610"
     CDS             25073..25783
                     /locus_tag="PKN31_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOA98459.1"
                     /translation="MQIFSLITKGGYVMYPIILCSIISLAIFLERWYVLRHSRERVDR
                     YLRSVKRAVAQRDWESVVDISKKNQSPPSRIILAGVRKYFQGLTGEAREAMEDVAMQE
                     LPVYEKRLSTLSVVASISPLLGLLGTVTGMIRTFDVISVVGVGKPAEMAGGISEALIT
                     TAAGLIVAIPTVLAHHYLSNLADKIISDIDRACTEVVEALEESANSHNTLPLGSENLR
                     EGEEENVPISVSEEETHS"
     gene            25743..26159
                     /locus_tag="PKN31_00615"
     CDS             25743..26159
                     /locus_tag="PKN31_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOA98460.1"
                     /translation="MFRFRSQKKRHIPEINMTPMIDVVLQLVIFFVVTTTFISVESGA
                     QVNLPSADFSRIEEAKTITVTITENNSIYIDGALVDEKELAQVAVAEVRQNPQAMVVI
                     EADKNVLHGKVVRVMDILRKSGAEKMAIATQPTEGR"
     gene            26156..27607
                     /locus_tag="PKN31_00620"
     CDS             26156..27607
                     /locus_tag="PKN31_00620"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOA98461.1"
                     /translation="MKQPFNEKRVWSFSLLFSIVINLVLVAVISIYWLSFHYFPLEEE
                     EPIVIELMEIPAPEQPVSEAPPQEAEVAELSPLKEVEDKKAELPPEVKSEIASEVQQK
                     KEEVKIAKPPVDLPPAETIIKKASPSGAQDFNPGEEIDVPENMSWQGVEAESTIKGKL
                     EEAGTSTRLSHLAQEGLKKVESTVGETLATGELSPPTSGVEKRSPFTKRPIAVIVENA
                     PAARPQSGLSKADIVYEILAEGGITRFLAIFNSEPAENVGPVRSARPYFILKAAENDA
                     IFVHSGGSVEAFTYLEMLAVDHIDEMKNFQPFWRTQDRHPPHNLYTSVASLRQEAQRL
                     GYNKPTRVGGFLVGDLGNPGGDSAPRLEISYTADYKVEFVYLPDKNVYRRLINGAPHV
                     DAAGGQAIQCSTIIVQVTEHQVKDEEGRLEIRFVGEGTGWVFRDGKVVPIRWEKSDLR
                     DKTRFYLADGEELKVNPGKVWIEVVDTRTKVVF"
     gene            27608..28360
                     /gene="lgt"
                     /locus_tag="PKN31_00625"
     CDS             27608..28360
                     /gene="lgt"
                     /locus_tag="PKN31_00625"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOA98462.1"
                     /translation="MYPVMFHLGSFPVYSYGVMLAAAILVGIFYAIRRAPRYQVSADA
                     VVEVSALCIIGGVVGARLLYVLINWEYYRENLAHIFLVREGGLTFYGGIAGALLLVIP
                     YVHYKKYSLPVFFDIFSPPIALGYAIARIGCFLNGCCYGRVCPFTSFPLAVKFPYLSG
                     FRYPTQLYSAGYSLIILFALLHLEKNKAFTGELFLDYLWLYGIARFLIEYLRDEPFSV
                     GGIFTLGQVACLSIVAFSLILREVIKRYGKES"
     gene            28344..29246
                     /gene="xerD"
                     /locus_tag="PKN31_00630"
     CDS             28344..29246
                     /gene="xerD"
                     /locus_tag="PKN31_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOA98463.1"
                     /translation="MERSLDAWVEAFLDFLFWEKRVADNTILGYGQDISSFVQFLKEK
                     EIASFGEVTLLVLEEFVSQESDRELEATSLARKISALRTFFSFLVREKEVPENLARLL
                     DTPRLERNLPQVLSLEEVEALLSACDKTKPRGLRDRAILELLYSSGLRVGEVVSLEFS
                     HLDLENRMLRLWGKGFKERMVPFGEEAHEAISLYLEKGRGEILKGRSSNYLFINGPSA
                     RPLTRQSVWNMVKRYAKRANLREDITPHTLRHTFATHLLENGADLRVVQEFLGHSDIS
                     TTQIYTHVSKKVLRETYDRAFPRK"
     gene            29264..30091
                     /locus_tag="PKN31_00635"
     CDS             29264..30091
                     /locus_tag="PKN31_00635"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOA98464.1"
                     /translation="MDDMKRKIEESYQFIKERIDFVPRVGIILGTGLGALVDDIEIER
                     VIPYEEIPHFPISTVETHRGNLVLGHLEGKPVVVMQGRFHYYEGYDMKQITFPVRVMR
                     KLGAEILLVSNAAGGMNRYMKRGDLMIIRDHINLLGDNPLRGPNDPELGPRFPDMSQA
                     YDRELGDLALQVALDNGIYVHQGVYVALAGPNLETPAEYRFLITIGADAVGMSTVPEV
                     IVARHSGMRVLGISCITDLAIDGVVEEVSFEDIVEAANSAEPKLTLITREVVKRLTL"
     gene            30095..30556
                     /locus_tag="PKN31_00640"
     CDS             30095..30556
                     /locus_tag="PKN31_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOA98465.1"
                     /translation="MVQELLEVALLAALPVSEVRGALPYGVLVRNLPFSWVVIVSFVF
                     NLLPFFLVMFALSFFLKFLLRWKWFSNFWSKYITGAQNRFKNYEKWGKWGIMLFVGIP
                     LPFTGVWTGSVVCFLVGLSWRDSLPFILGGLTMATILVSLAVLGGNYLFMR"
     gene            30565..>31346
                     /gene="mtnA"
                     /locus_tag="PKN31_00645"
     CDS             30565..>31346
                     /gene="mtnA"
                     /locus_tag="PKN31_00645"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOA98466.1"
                     /translation="MLKAVEWKGETFQFLDQTLLPLEEVYIETSDYREVEEAIRQLKI
                     RGAPLLGVTAAYGLCLGALEGEKGKVGDFFSFLEKVDVDLRTSRPTAVNISWALDRLK
                     KIWEQDMEDWSPRQVVNSLIDEAKKIEEEDYKNNENIARYGAQLLQDEDRVLTHCNTG
                     SLACSGWGTALGAISWAVLQEGKKIQVYADETRPLWQGARLTAFELMKNSIPVTVICD
                     NMAGYLMSQGLVDKVIVGADRVTLQGDAANKIGTYSLAVLAH"
BASE COUNT         8874 a         5551 c         7433 g         9488 t
ORIGIN      
        1 ctgccgggag aaaaagcagg agtttatctc ctgctttttt tgttggtggg atagagaaaa
       61 gaagttgcag gagctggtga gaggatgagc catttttctt aatccagcat atcggataaa
      121 agtttattat agtccggtta tagttacaac tgggcgaatg attgtataaa agctttcttc
      181 tggtaaaatt gaacagtaaa ggaggaaact gtggaaaata tttcccaacc atcttatctt
      241 actctaaaag agttgaatca gatattgaaa gatgtagtag aaaattgtta cttttctgcc
      301 cctctttggg tagtagcgga aattgctgag ataagattaa attctactac tggacactgt
      361 tacttggagt tggtagaaaa agaagatgac cgcgtggtag cccggatgaa ggggattatc
      421 tggtcttcta attatcctct ggtggtttcc tcttttctgg aagcaactgg taaaaacctg
      481 gaacggggaa tgaaagttct ctttttaact cgattgcgat ttcatcctgt tcatggtctg
      541 agtttggata ttaaagaagt cgacccttct tattccctgg gagagatgat gcgtaaaaga
      601 agagaaatat tagaatgcct ggagaaagaa gggtttttga ctcttaatcg ctcccttcct
      661 tttccccttt ttcctcagcg gattgcaata atttcttctc ctacagcagc gggatgggag
      721 gactttataa gtcatcttca gaacaatgtt tatggtttta ctttttcctg tcagttattc
      781 cccactttga tgcaaggaga agaggctgaa aactcccttt tttcttcttt ggaagagata
      841 aagagagaac aaaattattt tgatatagtg gtaataatta gaggaggggg ctccagtgtc
      901 gatttgagct gttttgatag ctacctttta ggaaaggcag tagccacctt tcctcttccg
      961 gttgtggtgg gtattggcca tgagcgggat gatacagttt tagattatgt ggctcatacc
     1021 agcctgaaga ctcccactgc agtagctgaa tttcttatta atcaaatgaa aatggtagag
     1081 gaagagatag aagaggaaat ggcggagtta aaagagataa tagaaaactt tatagagaaa
     1141 gaaagacgtg ttctaactgt tctggtcgat caatggacta gaagagtagg ggatttactt
     1201 ctttcatcgg ggaaagaact ttctttttgt cgggagagat tattggtaca ttctcaaagg
     1261 attctgggag aaataggaga aaaccagaga gcctatgaaa gacaattaaa aaatttagtg
     1321 gaagagagag tttccaagga gtgggaagcg ttaaaaagag ctgaacaggc aatacgtttg
     1381 ttgaatcccg agaatgttct ccgccggggt tacagtgtga cttatcgaga aggtaaagtg
     1441 gtgaaaagct ttagagattt acaagaagga gaaaatattt ctacccgcct gatggatggc
     1501 tggataaaaa gtaaagtgga ggagacttat ggaagagaac aatctgagct atagtgaggc
     1561 ttttcaggaa ctgaaatcca ttgtagatga actggagaat gaggaaatag aaatagacaa
     1621 tttgacggaa aaagttaaaa gagcctcttt cttgtaccgc tttttgaggg atcgtcttcg
     1681 agccacagaa gaagagataa ggaaagtctt ggaagaggaa gaaccttttt aagaaaacac
     1741 aagcttattt ttctttcaca ataaaatcct ggtttttaca acctttattt tcgaattgaa
     1801 tggtggaatg ttcgattcga aaatcctcga aaagaatttt ttgtatttct tcgcggagct
     1861 ggtcaatttc ttcccagctg gaacaggtag tagttacgtg aaattcagca aaatgttctt
     1921 tttcatttaa agaccataca tgaagatggt gaatattttt caccccgggc agagtttcta
     1981 aggctttttt caatttttcc aggtttaagt gaggaggtgc tccttgcata aggatattaa
     2041 ccacctcttt gaacattttc cagcttcctc gtaaaaggag aatcgatagt cctatgctga
     2101 ggagaggatc aataaaaaac catccccaat gcttaattaa tagagctcca attattactg
     2161 ctaaagaaga aaaggtgtct acaagaagat gccagaaaga acttcggagg ttaagacttt
     2221 ctcgtgaacc aggaaagagt aagaaaacgg aaaacaaatt tcccaaaaga cctattattg
     2281 ctatttctaa aaggatatgg gtttctatta cccggggaga gaaaagtctc tgaatagctt
     2341 ctataaaaat gtagccaagg gtggcaaata ttaacagaga attaacaaaa gcacccagaa
     2401 cttctgctct ccgatagcca taggttcttt gaggagtttc ttctttcaaa gaaaggcgga
     2461 gagagaaaag acttataaaa agagaggtgg tgtctcctaa attgtgtaga gcatcggaaa
     2521 gtagggctaa gcttcccgac catattcctc ctatgatttc gatcagggaa acgataaaat
     2581 tcaggattat ggcaactgaa agagcttttt cattgttatg aggatgagaa aatttatgat
     2641 tttcttccat aagaaaatgt aaataacaac atagtaaaaa gggggtgtta cccccttttt
     2701 actattctac tttgatttcc ttttctcctt tccacagtcc atggatattg cagtaggaag
     2761 tgacaaaaat agttcctggt tgattggttt taaaggaaaa gctcactcgt ggttcggtat
     2821 ataccggccc ttgattagga ccttcagtac tttctccatg agcagtgaat tcaaaatgtc
     2881 ctaactgaaa aggaaacttc cctcctgtag gcaggaaata gaccgctatc caccggatgt
     2941 gatgttcggt ggtattggga tgaggaatct cttttcctac agttgcggta aaattagtaa
     3001 gctctccttt tttaactgta tcaggtcctt caattaccgg aacatgtttt tcctctttcc
     3061 agtcggccga ttgaaaaagg tcgctaaaat tagtcatctt cttacctcct tagaaagctt
     3121 tgaatttttc tcgagaagct ccacagatgg gacatttttc cggagcctct ccttctgcgg
     3181 tgtaaccaca cactgaacat atctgaatgg ttcctaaagc tacatcttta ccgtcttcgg
     3241 cagcttcttt agcttttttg tacatctgga catgaatttt ttctgcttct aatgctccgt
     3301 gggtggttat ttctgcttct ttttctccct gaagtttagc tacagcattg taagcgggat
     3361 acatttcctc cacttcatat atttctccgg caatggccat atccagattt ttaactgtat
     3421 tgttgatata ccctaaagct cggtaatgat tggtagcgtg gacttcctca gcataagcga
     3481 tggccttaaa aaggcgagca atattaggct ttccctcttg ttctgctacc tgagaaaaaa
     3541 ttagatatct catgtaagct tgtgattcgc cagcataagc attttccaga ttctctcgag
     3601 tcattttgcg tttgataggc atatatgttc aggcctccct tcagtttggg atttttaaaa
     3661 gtaaactaaa ttgataatat tttattccac aggaggatgt tttaaaaacg gccgggcaaa
     3721 gaggactaaa agcttcccac tcctctggtc ttcacgacca atttcctttt acccggctat
     3781 tatacattat acaactttta atattttttg gaagctatct atctattaca gctctttttc
     3841 ttctggattt tttcttcgtt gagagaaaaa cagggggaat aaaaagggat atgtttcgtt
     3901 taaagctgtg acattatact attctaactt aagttttacg ttttttctgc aatttcctct
     3961 tattactcta tgttgcctgg gtgagccaaa gccgctatag aagaaaggag gtgttaaaaa
     4021 agtgcgagcc ttgaggcgat atgtagtcgt gggtttagtg atttttgtgg ccttctttct
     4081 gggaacctta gccggagata ttcctatttc tcattctaaa gaagatatga agtttgtggc
     4141 cgagagggta ggcttcactg ctcaatcttt ttatactgca ggaagcaatc aggtttacta
     4201 ccagattgaa agaaacctga aggctctgga tgaaataata aatatgatag aaaaagcaga
     4261 gaatcttgat gcgattatag accctatggt agacaaatta gaagagatta ccgtctctta
     4321 tgaacacatc tctgatctta gaccccagat agaagaagaa ttgagaggaa agatagaaga
     4381 acttcaggag ttgcttaaaa aatctaaaga ccagatcaga attctggaag atgaaaaatt
     4441 agaactggaa gaagagttaa aagaactatc tctggagcag gacgaggaga taagaaacat
     4501 caggagaaga agtttagaaa tgcaagtaga atttaaagcc aaagaaatcc aaatctggaa
     4561 ggaatttatt tctctggaag ataagattta tgagacaatt aagaatgctg accggactat
     4621 caaaaagttt cttctggttc tttctgagaa cgccaaagtc tatcgaggag ctttagaagt
     4681 ggttaggctc cagagggata tcaaaagttt ggttaatttc atgagcttgc aacaattctc
     4741 tgatgagcta atttctacct ggcaagacct tcagtatctt attgagcttc taactcagga
     4801 agttaataag cttcctgctt tccgggaata atgagtttcg gtattacgct atggagaaag
     4861 gagaaacaag aagagggttc aggaaaatag gatggtggat aattcttctg gtaatggtaa
     4921 tagttttttt cctcatttta ggaggcagta aaaggtggga atggttttcc cctccccgca
     4981 cctggtctga tatagctata cttgttcagg aagttgaaga aaaaactcaa aaattaaacc
     5041 agattttaca ggaagctctc caatcggtta gagaaaaaga aagcccttca gaaccatgat
     5101 taacctttta ccaaagactc ttcctcttct atctggagag gctcctcgga tttatcttct
     5161 tgttcttccc atctctgtct gaccgtaaca ggagtccact tgccgcagcg actaccccat
     5221 aaagccttaa tatcattttc ttcctttagt attactattt cgcaggcatt gggacaatct
     5281 tcacaggtaa agctccggct ttcaaactga cgaccaatca cttcaaatcc agaaaaagta
     5341 cttttttctg gagcatactc cagggctaaa atagctgctc cccaggctcc catgactcca
     5401 tgctcaggag gaacgacgat cttcttttta ccctggaaga aatctcggaa tgcctgaaca
     5461 atcccctcgt tggcagctac tcctccctgg aaaacgatgg ggtcttctat ggttttcccc
     5521 gtagctaaat tattgatgaa gttccgcact aaggcttcac aaagtccttt gacgatttct
     5581 ggtttgccaa aacctaattg ttgtttatga atcatatctg attcagcaaa aaccgtgcat
     5641 cttccagcaa tgcgcactgc ctgttcagcc cggagagcta attttccaaa ttcttcgatg
     5701 ggaacattga gacgtaccgc ttgatgctcc agaaaagaac ctgttccggc tgcgcaaacg
     5761 gtattcatag cgaagtcttc caccacgcca tctcgaatga taattaactt actgtcctgt
     5821 cctccaattt caaaaactgt tcgtacttca ggtactcggg tgatggctgc tatggcgtga
     5881 gcggtaattt cgtttttgac tatatctgct cctactacta tgccagccag gtgtcgggcg
     5941 ctaccggtac tacctactcc ttttatttct ccaccactgg gaaaatgtct ttctatgtat
     6001 tgtaaacctt ctttgagcgc tccaatcgga tcgccgctag tgcgcaaata gcaactggcc
     6061 agaatgtttt tttcttcgtc tataagcaca aacttagtgg ttactgagcc cacatccact
     6121 cctaaataaa ggtccattta actaacctcc accttttttt tcttcagttt ttttcttctt
     6181 tctaaaacat ctacaaatgc ttccactctg gtaactaatc ccgcctctcc agaatgttca
     6241 tctatggcta aatgcagagt aggtaagtcg taatccctgc ttactgcggt aagtatactt
     6301 ttagccacta cttcaggcat acaggtaaaa ggataaagat ggacaactcc atcaaagccc
     6361 cattgagcat agcgaatggc gctgcctact gtatcaattc cttctcctcc tacaaatctc
     6421 ttcaggtaag gtttggctag ctcccaggct tctaattttt cccgggaacg agtaaatggc
     6481 agggtgtgca aaacatactc taccgtagaa acagagttgt gtacgtatgc tcctagctct
     6541 cccaggatct tttccacatt gaaatttatt ttagtttcct gagctacata aatctctcct
     6601 actattccca cttttatgag gtcttctgtt tctttccgtt ctaaattatc taactttttc
     6661 atagtttctt ctttgatagc ctggaagctt ttttcattat ttacctgacg cagttgttcc
     6721 agcgctcctt ctactatttc gtctaatttt tgacggtctt ttacctgagc acgcagcgct
     6781 cttctttctg tctctaagtc ttcggtaaag cgcaaaactt tccaggccaa gctggctact
     6841 ttgataactc gggaggaaag gacccttccc ccggccatag tttgcagggc ttttataaaa
     6901 ggacgaatac catagcgagg atggtctaat aggacaaaat caacgttgta acctaaatct
     6961 tctaaaatta tattttgaat ttgtccgtaa taaccaaaac gacaaggccc cacccctccg
     7021 gccataaaaa tagtatcggc tcccagttca attccttcta caaagttagc cagagttact
     7081 ttgagaggaa agcaagcatt ctgaggagct agttttactc caatttcaat ggttttttga
     7141 gtaataggag gaggggttat gatttctacg cccagagtgg ggtagagaac tcctaaggcg
     7201 atatcaagat gaagcaaatg gggaaacgta gctttcaagg cttttatacc tccactccat
     7261 catatctaca aaagcttcta aacgagtaac caaccctgct tctccggtgt gctcgtcaat
     7321 ttctaaacgc aagtaaggga gagactcctt ttctcttttg agaagacgtt caattaactc
     7381 tcccactaaa gaatctggtc cacactcaaa ggaaaccaga tggataaacc ctctgacctc
     7441 cggttttctc tctttgagat ggtataaagt gctaccaatg atttcattag aaatagtcca
     7501 gaaactgggt ttaggaatag tagagagata gtatcttttc ttttcctcat ctaccatatc
     7561 gggagtgatt accgagtagt tgagatcgtt gactttttcg gtgatattca tatttatata
     7621 gctatcactc aaaagataag tatggcctaa aaggacaatt ttcttctccc ggagaggaag
     7681 gggatttttg tggtcaatca agtcttcggg gagcatgccc tttttgaggt tggcctgata
     7741 atctttatga gctttttctg ctaaattcag agctttcata attttacttt ctttagtagt
     7801 aaagtgttta ccaatgttta aaaatcctcg cctccagcct cgcatcccct cttcccgcat
     7861 attaatttcg tcaccaatta tcctttcttc aggaatatta aagaggttac gggctaaatc
     7921 aggaagagct aatattttgg gacaattata ttctctcctg tgaaaactga ctattcgggg
     7981 aataaatatg aggtccactc ggtctttaag ataaagaaca tgtcccaaaa acactttggc
     8041 tggaaaacaa atttcctcca ccgcgtaggt taagcctttt tggataatgc ctttgttggt
     8101 gggaggagaa attactactt ctgctcccag ttcttccaga aaagttttcc agagggcgta
     8161 aaaacgatag aataaaagac cccgcggaat gcctaccttc attttttcac ctacttttaa
     8221 aaacattcag gctttattat acaacttaaa tcctaaaaaa caaaaaactc ttcttcctac
     8281 aaatccaatt cttctatcat tctaatccat attcccctct gctctttttc ttgaaatcca
     8341 ttatggagat agaactcttg ggctttgcga tttttttctc ctacccacaa ctctattttc
     8401 tttctccctc gagaaagggc aatctggatt gcttcctcta tcaatttttt tcctactccc
     8461 cttccctgaa attggggatc taccacgagt tcatgaatct cagccagata ttcttttcct
     8521 tctctccagt tagcatgcat tcctaataat cctactggtt tatttccctc aaaaactact
     8581 aaaaaaccct caggatctcc cttccatagc caggagaaat aagccgctat tttacagtag
     8641 ctgtcatctc gatattcagg aagattctca taagctcgct gataaatgga aacaaggtca
     8701 ggaattaact ctttaaattg acaataactc aaatttttct caatagtcac ttctaccaat
     8761 ttgcttccct tccctctgaa attgaagatg aatttttcca ttattttttt ctgttatatt
     8821 taaccccaaa atcccttgca aaatccctct actcagtgtt ttgcttttct tatttgtacc
     8881 ttgatattat tatgtcaacc aatttaaccg tcaccgcgag catcagcttt gcagcctgac
     8941 aatattgaga gaaaaatata gagcacaaga gattaaaaaa tttagaatat caacgctggg
     9001 gggaatgcgt tccggcgagc gtggtgattc cgagctttta acctctgatg tagcttgcct
     9061 ttaaaagttt gagcaattta ataacttgtt tgctccataa tattatacgc cgttattggg
     9121 aaggatacga gaagtcatta cagtaacaaa aatagttaca taggagaagg aagatagaat
     9181 ttttctttct ctccataagc tgttataatt gtctaagcct attttaagcc aataatgttc
     9241 ttgagaagag tttctcttga aataaacagg gcgaatgctg gtttttgata ataaatagat
     9301 gaaagttaag tagaaggagg atagaaaaaa acagtactat gggtaatggt tatcgagtgg
     9361 gagtagtaga taacttagac aagctttttg cagttttacc tgatcatatc agaattcttt
     9421 tggaagagcg cgaaaatgct gatgatttga tagaggtggt aatggatctg gggcgtcctc
     9481 ccgaagcccg ttttgccaaa ggttttgtga ttctctctga taggataata gagagggaag
     9541 atcttgatta cgtagttcaa agggtgggaa tgtttactcg agataaccga gcaggtattg
     9601 agcggactct tcaccgtatt tcctgtattc gtaaccgatt gggaaatatt gtagggctca
     9661 ctcttcgggt agggagagca gtatatggta ctattgatat aatcagagat gttatagtat
     9721 caggtaaaaa tattttgctt ttggggccac cgggagtggg taaaactacc aaacttcgcg
     9781 aggtagctcg agtgttgagt gatgagttcc agagaagggt ggtagtagtg gatacttcta
     9841 atgaaatagc tggtgatgga gatattcctc accctgctat tggtcgagct cgtcggatgc
     9901 aggtatcatc tcctgagaaa cagcatgatg tgatgattga ggcggtagaa aaccacatgc
     9961 ctgaggttat catcgtggac gaaattggaa gagaagagga agcaagagcc tgccgaacta
    10021 ttgccgagag aggagtacag cttattgcta cagctcatgg caacactctg aaaaacctcc
    10081 tattgaatcc tactttaagc gatctggtgg gaggaattca gtcagtgata ctgagtgacg
    10141 aggaagctca tcgtcggggg agtcagaaag cggtactgga gcgcaaagct attcctactt
    10201 ttgatgtggt tattgaattg cgtgaccgag atacactggg catttaccat gatactgctc
    10261 aggcagtaga tttgttgttg cgaggttatg accctcgacc agaggtgaga aaaagaacca
    10321 aagagggaac ggttaaggtg gtggaagagg acaaaagggc ctcttctttt gtagatgcga
    10381 aaatggcaca ggagacagaa gtagttcctg aattttttga gagtcaatta ctacgggttt
    10441 acctcttcgc tgtaagtaaa aattatgtac agagagccat tgctcacgct aaggttcctc
    10501 ttgagatagt agaagatatc aatcaagcag atattcttct cacccttaaa agtcgctatc
    10561 gcaataaagg aagcaagata agggaggcag agaaccgagg cataccggtt cacgtggtgc
    10621 gtagcaacac ttataatcag atttataagt ttgtacagga ccttttcggt tatagtgatc
    10681 tctcttcagg caatggagac tcgggattaa tagaggcaga aagagcggct cggcaagtgc
    10741 tcaaagtagg ggaggctata gagctttctc ctcgtaactc ttcagtgagg cgcttgcagc
    10801 ataaaattgc cgagcgttat caccttttct ctaaaagcgt aggagaggaa cccttccgcc
    10861 gggtcgtaat ctacccccgt attctggaag aaaaccgtca tgaatgaaag acctggtttt
    10921 ttgattactt tggaaggaat tgagggaagc ggaaaaacca ctcatgctcg tcggttattt
    10981 tcttttctga aagaaagtgg tattccctgt ttgttgactc gagagccagg gggaacgcct
    11041 atgggagaaa aaataagaga tatattgcta aatcccgaaa gcggggtgat cgaccctctt
    11101 gccgaaactt ttctttttga ggcttcacgt cgagagcatg taaaagaagt gataaatcct
    11161 gctcttcatg aaggtaaagt ggtgatatgt gtccgattca ccgattccac cctggcttat
    11221 caggggtatg gtcggggagt gcctctttct tatctttgct atttaaatga tattgccacc
    11281 gagggggtta tgcctgattt aactgtgctt tttgatgtag aaccggaagt gggcctggct
    11341 cgctcttttt cccataccga agaggaagag ctgcgttttg aaaaagaatt tttagcttct
    11401 aaggaatttc tctttgctgt tcgaaatggc tatctccagc tggctaaaga atctccccag
    11461 cgttttgtgg tagtcgatac tactgattat tctgaagaag aggttttttt tattattcga
    11521 gacactatat ggcaaagatt aaggggaagg gggtgaattt gtgtctccta atttactttt
    11581 aatatgtgtg gtgcgagatc aagatgttgc taagctctta gataatctgc gagataggaa
    11641 catttctttc actaaattag cttctaccgg tggtttcttg cgggaaggta ataccactct
    11701 tttgatcggt atagaggaag agcgcaaaga agaagtgaga aaattaatca gagaaacatg
    11761 ttctcgccgg gaagagattg tagaatctac tgttcctctc aatgaaccta ttggtccttt
    11821 tgttccccag aggattaaag tagtgagagg aggaggagtt ctttttgaag ttcccatttt
    11881 gtattatgag agattttgac ctctctcgcg ggaagataag atgagttgat ttgatgagct
    11941 ggtctgaaat ttgtggtcac gaaagacaga aggagttttt cgagcgtgct gttctttcag
    12001 gaagagtcgt tcatgcttat cttttctccg gtccttcccg ggtgggaaaa accacttttg
    12061 ctctggagtt ggttaagctt cttaattgtt tgaatcctac tgaagaagga agttgtgatc
    12121 gatgcgaaaa ctgccggttg ataattaatc ttacccaccc cgatatttct tttctctctc
    12181 cttcgggtga ttatttaaaa atagatgaaa taagaagttt tgtgaggtgg ctcaatttca
    12241 agccgctaat gggcaaaatg aaagtaggga taatagaaga ggcagaaaaa ctgactgaag
    12301 aggcggctaa ttgtctttta aagactttag aagaaccacc tggggagagc atggtgattt
    12361 tgatcacttc tcaacgggct tctcttttac ccactataat ttctcgttgt caagtggtgg
    12421 agttttctcc tctttcttct tcagaaatca gtaattattt ggtggagaag aagggatgga
    12481 aggaagaaga ggccaggaga gcatcttcct tttcccaggg tagcctggga aaagcggaag
    12541 agatcaagga gggtagaagc ggtctggagg ataaattttg ggaattgtgg tacaaactga
    12601 aaagcgagag ggattctctt tctctgggct cctggttcta tgataataag gaacagctgc
    12661 ctctgattat gattttttta gagcagtatt tgagagattt gtgggtgtgg aaggtaacgg
    12721 agggtcaatg ctctaatttg cttttttttg aaaaagaaaa agtacaggaa gaagcgaaag
    12781 aacaagaagc agaaacctgg aggaggttaa taattttagt ggataatttg atctctgata
    12841 tttctggtaa tctaaattgg gatatagttt tatgtagatt tctttttttg tggagaggtg
    12901 aatggaatcg tgcctcaagt agtaggaatt aaattcgaga acaatcctaa aatttatcat
    12961 tttgaaaccc gaggtttgga tttacgctcg ggggataagt gtgtggtaga aacttctata
    13021 ggcttagaaa taggtattgt agcaaaggaa gtttttttag ctcctgagga taattttaag
    13081 ccggtaattc ggaaagctca aaaaatagat ttagatcagg cagaagctaa tcgggaaaaa
    13141 gaaaaaatag ctctccagaa agctgaagaa ttggttgctc actaccagat cccgatgaag
    13201 attttacggg ttcactatac tctggatcga ggtaagttaa ctttttattt tggttcagaa
    13261 gaaaggattg actttcggca gctggttaaa gagttagccg ctatttttca aactcgcatt
    13321 gagctacggc aagtgggggt gagagatgaa gctcggatga tgggtggatg tgggatgtgt
    13381 gggcgagagt tgtgttgcaa tactttttta accaatttcg accccatctc tattaagatg
    13441 gctaaggaac aaaatttagt tcttaactcg gctaaaattt ctggggtctg tgggcgtctt
    13501 atgtgctgtc tggcttttga atatccccta taccgtcggc ttattcatcg cctgcccaaa
    13561 aaaggtagca agataatcac tccctgggga ttggcaaaaa ttttagaagt ggatattttt
    13621 aaagatagta tttccctgga gctggaagat gggaaaaagg ttcgaatcac cgaggaagaa
    13681 tatcatcgtt attttttata aggtggtgaa gaaaggaact ttttaatgtt ccaggaatat
    13741 tttggggata gaaagtgagt gagataaatg gacaaagctg aagttcaggt ttggtatcag
    13801 ggcttaactt tggaagaaga aaaattgtgg tttgaggaaa aggttaaaag ttgtgaaagt
    13861 gggcttcttc gttttgccta tcatctgagt ggcaatatgg ataaagcccg ggatttggtg
    13921 caagaagctt ttttacgagc ttaccagtat cgacacagtt ataatgccaa ctattctttc
    13981 gaaagctgga tgtctgctat tctgcttaat gttcatcgtc agcgttttaa aaaagagaaa
    14041 tttttcaaaa tctttcatcc gggaagggaa cgagaagatg gagaagaaac cgaagtttgg
    14101 gattttctgg aatctaaagg ggaaggcccg gaagagagaa ctttgaaaag agaggttctt
    14161 agttttcttc aagaggctat tgaaaagctt cctcctcaga tgaaagaagt aatagtgctc
    14221 tgtgatgtga tgaactattc ttatgaggaa gccagcgaaa taattggctg tcccttagga
    14281 acagttcgct cccgacttca tagagctcgg aagaaagtga gaagtttagt agaagagagc
    14341 tatggagagg atttcctctc tatttgggga tgagaatatg aattgcaaag aagcaggaaa
    14401 gatgctttct gcttatctgg atgaagaatt gactgccgaa aaatacaggt ctttgaagag
    14461 acatctgatg tcgtgttcta cctgtcaaga ggagctggag aagcttcggg taattaggaa
    14521 tctgatgaga gatttagata gtgattccaa ttttaattgg aattttaagg ttgattggga
    14581 agctgtaagg agacaggcaa gagcgatgag aagaagagag atggctttct tttctcttgc
    14641 cgccgcttta gtgattatag ctttattttt tgttttttct caagagcaag ctcaatggga
    14701 aatcgaggtt atgcatcctg aagttctggt agatcttcac cagagcttaa gtggtaacca
    14761 gttggatata cctttaaaac aagaaaccaa ttttatcgga accttggagc tggtctccgg
    14821 tggtaggtag tgagaaaatc tgttttttgg attttcgtag ggtttagttt ttttcttttg
    14881 attttttgct ggggtttttc gtctttttct gtagctggag cggaaatagt caatccggaa
    14941 gaagctttat cctttctgaa agagatggtc aagaaatcta ctgaccgcga ttattggggg
    15001 atatggtcca atcgagattg tcgttcagga agagaagatt tttacgaagt tattcatctg
    15061 aaatatattg gtttagcgtg gagaaactta aacgagggag acctggttac tgttaaaaag
    15121 gggaactacc gttatgtagt ggacctggca agtggagaag ttaaaggagt ttaccctctt
    15181 tacactcttc ctttccttcc ccctgaagaa aacaatctgg aattgctttt agaaaattat
    15241 ctattagatt tgcaagatag aaaaggacga gtgatttctc gccatactgg caaggtcatc
    15301 cgttcctttt cagtagatga aaagggctca ctggtttctc aggcttttta cacttcagag
    15361 gggaagctaa aggaggaggg aagttttgtg tatcgggatt attctcctga ttacacccag
    15421 ttaataaaat atgtggaagc catgaaaaaa ccttctgaca ttaccatacc ttctctcagt
    15481 gttagcggta agaagctgtt ccagccttct ctattacctc cgggttttca tctaaaacaa
    15541 gtttatttag tgaagaacga ggatcgagac tggtaccaga tggtttatac tgatggttta
    15601 cagtattttt ctttatggca aagtgtttat cccctgacat tatccgggga aaaaagaaac
    15661 agaaaaatct ctttgaaaaa ggaggatgat atagttacct tgatgggaga aaagagcgga
    15721 ttttatttag ctttgatggg aaattttgat ttagagacga taacgcagat tttcaattct
    15781 attacctggg aaggaggtaa ttagctgtgt ggattaaaaa aactaagcta ttaagctttt
    15841 ggacggtaat tttgctggtt tctctggtgg ggggagtagc ctcagcagtg agtgcgatac
    15901 ctgagactac caacatcgtg gctgacattg tggaaaaggt cagcccggcg gtagttaaca
    15961 tagatactct taaaatggcc acttatcgtt ctccccttgc tccttttttt agtgatccct
    16021 tttttcggcg gttttttgaa gggatagtac cggaggaaag gcaggttccg attaaaggtt
    16081 tgggcaccgg ctttgtcttt cgttccgatg gctatatttt aaccaatgaa catgtggtga
    16141 gcggagcaga tgaaataaaa gttacttttt tagacgggag agagtttgaa ggtaaggtta
    16201 ttggttcgga tactctcact gatatagcgg tggtaaaaat tgaagctcag ggtcttccta
    16261 ctatttcttt gggcgactcc gatgctgccc gagtgggaga atggataatt gccattggca
    16321 atccttatgg actttctcac actgttactg taggggtttt gagtgccaaa ggcagaccta
    16381 tagctgctgg agattcaggt caggaatatg agaatttctt gcagactgat gctgctatta
    16441 atcctggaaa cagtggagga cctcttctta acttggaagg agaagctatt ggaataaata
    16501 ccgctatcct tccttatgcg cagggtattg gatttgccat tcctataaat atggctaaat
    16561 ctatcctgga tcagctcata actgaaggaa gagtaataag ggcctggctg ggggtagtca
    16621 ttcaaaacct cactcctgaa atggctgaga aatttggttt aaaagaggcc gaaggagcgt
    16681 tggtggcaga catttctccc gatagtccgg cagaaaaagc aggtattaaa agaggcgatg
    16741 tgattttaaa agtggatgga atagaaactc ctgatataaa aactctgcag caaaccattc
    16801 gctctcatcg cccgggagat gtggttgatg ttgaaatctg gagagatggg aaagttatca
    16861 ccttaaaagt aactttagcg gaattagaag aaagtatttc cgtactacca gttactgaaa
    16921 agatagattt gggcttggaa gtaggggaaa ttactcctga cctcgttgat aggtatgctt
    16981 tgcgagagaa gagtggagtg gtgatagtgg gaataaaagc tggtggtcct gctgatgatg
    17041 ctggtctgcg gcccggagat gtaattctgg aagtcaatcg ggaagaaatt aaaaacttaa
    17101 aggattggga agctgctatc agtgaaatag aaccaggaga gaccgttctt ttgctcatta
    17161 gtagggcagg acagacctat tttgttcctc tcaaagctga agaaccacag tcttagaatt
    17221 tatggaagta ggaaaggagt ggggcaaatt atatttttgc cccactcctt tgggaaattt
    17281 gcaggatatt actctgcgca ctcttcaggt gcttcaggaa gttgatttca tcgcttgtga
    17341 ggacacccgg gttacgttga agattctcaa ccactatcat attcgaaaac ctctttttag
    17401 ctatcattcc tacaacttaa gagaggttag ttcccgaata gtaaaattac ttaaagaggg
    17461 taagaacata gctctcgttt ctgacgcggg aatgccgggg attcaggatc caggaatgga
    17521 attggtgaat attttggaag aagaggggtt gagtttcgaa gttcttcctg gtccttctgc
    17581 tttggtagta gcagcagttt actccggttt ttccttctcc ggttttactt ttttgggatt
    17641 tttacctcga aagaaaaaag agagaaggaa aattttagaa caacttcttt atcttccccc
    17701 ggcaatagtt atttatgagt ctcctcatcg agtggtgtct gccctggaag aaattgagga
    17761 aatagctggg agcgagagaa ggattgtttt tttgcgggaa ctaactaaaa tccacgagga
    17821 aatattgagg ggaaaacttg gagatatttt aggaatttta tctcagcgag aaaagataaa
    17881 gggagaagtt gtgctggtga tagagggaga aaagatagag aaacagatac cgcctgaggc
    17941 ggagaggtta gttaagtctc tttctcggca ggggatttct cctcggcaaa tattggaagt
    18001 ggtgagtgat gcgttttcga taagtaaaaa taagctgaaa gaggttttgg aaagggaaaa
    18061 gtcgagttga ttttgaagag gaaaactgct ttaattacca atggtataaa tttgacttcc
    18121 tgaatgagga ggtagtgatt ctgaaaagaa aaaagcggga aggagcagaa atagtacttt
    18181 ttttaattgc cttttcttct ctgttttttt tgattggcat agtggttgtt ctctttgcag
    18241 aagggttgcc agtttttgct catgtttccc tttctcagtt tttattaggt agaagatggt
    18301 atcctacttc ctctcctccc cagtttggag cgttgcctct tatcttgggg tctctattgg
    18361 ttactgctgg agggttgctt tttgctgttc ctttaggcct ttttgctggt atatttttag
    18421 ctgagctttg ccctcctcgg ggaagggaga ttcttaaacc gattgttgaa ctgttagccg
    18481 acattccctc cgttgtctat ggcttttttg gagtgttggt tgttgctccc tttattcgcg
    18541 acttcctttc tctttctacc ggtctcactg ctttcactgc gtctttgatt ttaggtataa
    18601 tggctgtgcc ttccataatc agcgtgatag aagatgccat cacttcagtt cctcgcagct
    18661 acaaagaagc atcgcttgct ctgggagcaa ctcactggga gactttgagt caagtggttt
    18721 tacctgctgc ctcttcagga gttggagcag caatcatttt aggagcgggg aggattatag
    18781 gagaaaccat ggcggtttta atggtagcag gaggagctcc ggttatacct aaaagttttt
    18841 ttcagccggt acgtacttta actgcaacta ttgctgtaga gatgggtgaa gctccagttg
    18901 gtagtccaca ctaccactcc ctttttggtc tggctatcat tctcttcctg tttactcttg
    18961 ttctcaatat tttagtggag agtttagcta caagggggag agaagggagg actagatgat
    19021 aaaggaagag agcttgaaaa aaagatattt agttcagaat atatggttta ctatattttg
    19081 gttatccatg gttttgattg ctgcagtttt gggtctaatt ttatatttca tcctgtcccg
    19141 gggaataaaa gtaatcaatt gggaattttt gactgctgct ccccgtcggg gtatgactga
    19201 ggggggtatt tttcctgcta ttgtaggaac tttttacctg gtattgggta gtgtagtagt
    19261 gtctctacct ttaggtgttt tttctgctat ttatttaacg gagtatgctt ctcccggtca
    19321 tccgatagtg agagctattc gtactgccat ccattgcctg gcaggggtgc cttcggtagt
    19381 ttttggttta tttggcctcg cggttttcgt taaaattttt ggttttggtg tatctcttct
    19441 ttctggtgct cttaccctgg gcattatgtc tcttcctgtg gtcattacct cagtagagga
    19501 agctttaaaa acagttcctc tttcttttcg agaggcatct cttgccctgg gagccaataa
    19561 atggactact atctggcggg tggttttacc cagttccctg ccaggaatcc ttactggttt
    19621 aattcttgct gtgggtagag tagcagggga aactgccccc atcatgttta ccgctgctgt
    19681 attttatgct cgaaggttac ctcaatctct ttttgacccg gttatggctc ttccttatca
    19741 tatttatgga ttgatgactg agggtacaag gccagataaa cagattcctc ttgcttacgg
    19801 gacagcggtg gttttaattt tactggttgt agctattgat tttatcgcta tttatctgcg
    19861 aggaagggct aggcgagcta gaaagtggtg aaaaatggag gaaattaaac tacaaattaa
    19921 ggatttaaat gtattttttg gagaaaagca agtgctccat caaattaatt taaatattcc
    19981 agcgaacaag gttacggcca ttattggccc ttcgggttgt ggtaagtcta ccttgctgcg
    20041 tagcatcaat cggatgaacg attttcttga ggatgtgagg gtagagggag agattatttt
    20101 tgatgggagc aatatttatg atgaaagtgt agacccggtg gtagtgagaa gacgcatcgg
    20161 tatggttttt caagtgccca atccctttcc caaaagtatt tttgaaaatg tagcttacgg
    20221 tttgcgaatt cagggatgga aggataaaaa agccatcgaa gaacaagtgg taaaaagtct
    20281 acaggcggca gctctgtggg atgaggtgaa agataggctt cactcttcgg cttttgatct
    20341 ttctggaggt cagagacaac gtctttgtat tgctcgagcc atagcagtgg agccagaaat
    20401 cttgcttatg gatgagccag cttcagcgtt agaccctatt gctaccgcca gagtagaaga
    20461 attaatcagg agtttaatag aaaaatacac catcgtttta gttactcata atatgcaaga
    20521 agcggctcgt acttcggact atactgcctt tctcctttta ggaagactca tagaatttgg
    20581 tctcactaat cagattttca ccaaccctca aaagaaagaa accgaagact accttaccgg
    20641 aagatttggc taatagggag gaactgaaag aagcaaagta ggataacata agagtgtggt
    20701 ttctcggtag aggaggtttt ctaactatgc aaattttttc tttgataact aaaggcggat
    20761 acgttatgta tcccattatt ctctgttcta taatttctct ggctattttt ctagaaagat
    20821 ggtatgtact gcgtcattcc agagaaagag tagataggta tttacgatcg gtaaaaagag
    20881 ctgtggctca gagagattgg gaatcagtag tagatatcag taaaaaaaat caatctcctc
    20941 cttctcggat aattcttgct ggagttcgga agtactttca aggtttaacc ggagaagccc
    21001 gagaggccat ggaagacgtg gctatgcagg aattgccagt ttatgagaag aggctgtcta
    21061 ctctttcagt agtagccagt atctctcctc ttttagggtt actgggcaca gttactggta
    21121 tgatcaggac ttttgatgtg atatcagtag tgggagttgg taaaccagcg gagatggccg
    21181 ggggaatatc ggaggctttg attaccaccg ctgcaggatt gattgtggct attcctactg
    21241 ttcttgctca tcattatctc tctaatttag ctgataaaat catcagtgat atcgatagag
    21301 cctgtactga agtggtagaa gcgctggaag aaagcgccaa ctctcataat actttacctt
    21361 taggaagtga aaatctgagg gaaggagaag aggagaatgt tccgatttcg gtctcagaag
    21421 aagaaagcta ttcctagcat tgataggata aaaatatttc ttcttttcct tttgctgttt
    21481 gtttctttct tttctgggat agttgaagcg caagagagag taaaagtagc tgtcttacct
    21541 ttggtgggaa aagatagaga agatgcttat tggggctatt ttttgcgaga tatcataaaa
    21601 atgggtttgg aaggtgaagg tggggtagaa atatgcgact cgctgcttgc tgatgagata
    21661 gtgagagaag cacggatttc ctggtctgat gttttgatgc cttctactgc taaaattata
    21721 ggggaaagga ttgggtgttc ttatcttttg accggttctt ttcgttatcg tctgatggca
    21781 ggtagagaga gaattttggt aagcgctcgt attttcgaga tagaaagtgg tgattatctg
    21841 gatataccca gtgaaatatt tgatatcgat agattaccag atctcgttga atatatagtg
    21901 ggagagtctt tatactttat gggtttggat tacagagcac cgatttatct tccccctttc
    21961 gcgatagata accttttacc tctctaccgg ggagtgcaaa agatggatga ggcacttgct
    22021 acttatggag aggcacagta tccggataag cctctatgga aagaagcttt ttctttagca
    22081 gaagaaacca tagaaaaaga gcctgagtat ttagatgctt atagttatct tgcctatatg
    22141 tatagaaaaa cgagctggtg ggctaaggag gtggaaacct gggatctata tttgcaaaag
    22201 ctcgaggaga gaggagagga ggttgacaac cgacaaattt cccaaactta tcttcgctta
    22261 gcttattcct atttctatca gaagaattta aatttagctc gagaaagttt acaaaaagca
    22321 gtagaaatca gccctcaatg ggctgaacct tatctgttgt gggcgagaat tgaatatgaa
    22381 gatggcaata tagaggaggc agaaagactt tattctcaag cttttgagtt ggatccctcc
    22441 cttaaagagg ctgaatattt tgcccaattg gcagggaaag ctaaagtcta cggtaagtca
    22501 gcttatgaag cctatgtagc tggttaccaa tcttttgctg ctggaaacct ggaaaaagca
    22561 gaacagtatc tacgagaagc tattcatcta aacccggaaa tgaaagaagc ttactactgg
    22621 ctgggaagaa ctctctatga cgccggaaag tcaaaagagg cagaaaaagc ctgggaaaag
    22681 gttttagaaa ttgacccctt ccatagccag gctcgccggt tcttagataa aacacagcag
    22741 gaagtaaaat atggtagaga agcgctaaat tattttcgcc agggttatga gctttacgag
    22801 aagggtctat atgaggaagc agcaggacta tttcgtcagg cactaaaagt taatcctgtg
    22861 tttcctgatg ctcatgatta tttggcccgc tgttattatc gcctgggaaa aactgaagaa
    22921 tatgtaagag aaagagaaga aagcgctaag cttttagaag acgcagagga gaaggcttgg
    22981 caatattatc aagtgggtta cgagctgttc tcctggggag aaaaagaaaa agctcttcaa
    23041 acactggaaa aagcgataaa agaagatcct tctttagctg atgctcatct tttattggga
    23101 gagctctatg gagagaaagg aaactgggct aaatctggag aacattatgt tcaggctctc
    23161 gaaggattag aaggagaaag ccggggttta gctctatggg gagctgcggt agccagtttt
    23221 cacttagaaa attgggaaga gtctctttcc ctcttgaacg agctggtagt taattatcct
    23281 tatggtgagt tcatagaaga ggcagaagta atgaggatgg aagctctggt gagagaagga
    23341 aaatatgagg aggctcaacg tagcttccag caatttctgg ttcgttttcc ccagagtcct
    23401 ttcctggagc gagctcagtt ttggtatgct tccagttttt atcaggctaa agactgggga
    23461 aatgctcaaa aattcctgga agaattcctc cgcctttatc cccagagttc ctttcgctct
    23521 tctaccatag agatgctggg ctatacttat cgaaatctgg ggaaagaagg ggaagctcaa
    23581 aaatactttt ctcaactgga gggagaagaa ggagattttc taactgctga tgcttattat
    23641 cgagaaaaag attggaagaa agccatttct tcttttgagg actacctttc ccgttatcct
    23701 caaggtaagt tttctttaga ggctcggttt aagttagctt cttcttacct ggaaactggt
    23761 aatcctgata aggctcagga gctcataaga ggatgggaag gagaactaca aaataagttc
    23821 aagaaagatt tcttgccttt tagcattaag ttacacgctc aaaaagaaga atggagagag
    23881 gttaaagagg aaattctacg tttggaaaaa gaaagcggca agttggagga agaatattat
    23941 ttacttttag ccctggctta cactaactta ggagaagaag aagaagccaa aaatgtcctc
    24001 gctcgggcgg gtaaagatcc agaagagata ttagcgagcc ctgagaaaga ggaagcacag
    24061 gaaatattgg acctgatgga gagaggagat taccattcgg ctctttctcg cctcaaagtt
    24121 ctcagggaag aggaaagtcc tttaattagc aaatcaatga tcgactttct ggagggaaag
    24181 tgttgttatc agctgggaga tttctctcag gcttttaacc tcctttctcg ggctctcgca
    24241 tcccctgagg aaagctttaa ggaagaggct ctcttttacc tctttaattc tgcttataag
    24301 ctggagagat gggataaagt agtagaagcc tattctcaga tgaaaaataa agatgactca
    24361 tcagtacgtt ttcgagcggc tttagcttat tattactcgg gtaactatga agaaagcata
    24421 cctattttga gaagtttgca ggacgaccct gacctttctt cttctgtaat ttcgcttttg
    24481 gtggaagagc tgtttcttct aaaggattat aatgagtgcg ggaaagaagc agagaaattt
    24541 ttgattactt tccctcagga cccccaaagg gagagagttc tttatcttgc ttcctgggct
    24601 ttttattatt ctgacgacct ggagaaagct gaagaatcca taaaatctta ccagacggaa
    24661 tttccagggg gaaaatacag agaggagctt tcctctcttt tagccgacct ttatttgttt
    24721 caaggagagt tagatgaagc gataacaatt ttaaaagaaa tggagtggag cagtgaccct
    24781 cagcggaaac tttatacctg gtatagatta ggtaatgcat atttagggaa ggaggatttt
    24841 gcccgggcct ttccttattt cgagcgtttg tttcaagcag gacagagtga gtatcaaact
    24901 gcagctggtt actggtgggg agtttgttta gaatatctgg ataggttgga ggaagcaaaa
    24961 gaagtttacc gggctgttat tgagaccggg caagaagacc agtgggtgtc cagggctcag
    25021 gaaagatggg atgtgctgac cagatgaaaa ggaggataga ggttttctaa ctatgcaaat
    25081 tttttctttg ataactaaag gcggatacgt tatgtatccc attattctct gttctataat
    25141 ttctctggct atttttctag aaagatggta tgtactgcgt cattccagag aaagagtaga
    25201 taggtattta cgatcggtaa aaagagctgt ggctcagaga gattgggaat cagtagtaga
    25261 tatcagtaaa aaaaatcaat ctcctccttc tcggataatt cttgctggag ttcggaagta
    25321 ctttcaaggt ttaaccggag aagcccgaga ggccatggaa gacgtggcta tgcaggaatt
    25381 gccagtttat gagaagaggc tgtctactct ttcagtagta gccagtatct ctcctctttt
    25441 agggttactg ggcacagtta ctggtatgat caggactttt gatgtgatat cagtagtggg
    25501 agttggtaaa ccagcggaga tggccggggg aatatcggag gctttgatta ccaccgctgc
    25561 aggattgatt gtggctattc ctactgttct tgctcatcat tatctctcta atttagctga
    25621 taaaatcatc agtgatatcg atagagcctg tactgaagtg gtagaagcgc tggaagaaag
    25681 cgccaactct cataatactt tacctttagg aagtgaaaat ctgagggaag gagaagagga
    25741 gaatgttccg atttcggtct cagaagaaga gacacattcc tgaaataaat atgactccta
    25801 tgatagatgt ggttttgcag ttggtaatct tttttgtagt taccactact tttatcagtg
    25861 tagaaagcgg ggcacaggtt aatctgcctt cagctgattt ttctcgaata gaagaagcaa
    25921 aaactataac ggtaaccata accgagaata actctattta tatagatggt gccctggtgg
    25981 atgaaaagga attggcccag gtggcggtgg ctgaagtccg ccaaaacccc caagctatgg
    26041 tggttattga agcagacaaa aacgttctcc atggaaaagt ggtaagagta atggatattt
    26101 taaggaaatc gggggcggag aaaatggcta ttgccactca acccacggag ggacgatgaa
    26161 acaacctttc aatgagaaga gagtatggtc tttttctctt cttttttcta tagtaatcaa
    26221 tttagtttta gtggcggtaa tatccatcta ttggctttct tttcactatt ttcctctcga
    26281 ggaggaagag ccgatagtaa tagagttgat ggaaattccc gctcctgaac aaccagtaag
    26341 cgaagcccca cctcaggaag cagaagtggc agagttaagt cctctcaaag aagtggaaga
    26401 taaaaaagca gaattgcctc cagaggtaaa aagcgagatt gcctcagaag tgcaacaaaa
    26461 aaaggaagag gtaaaaattg ctaaacctcc tgtggattta cctccagcag aaaccataat
    26521 aaagaaagct tctccttctg gtgctcagga ctttaaccct ggagaagaaa ttgatgttcc
    26581 agaaaatatg agctggcagg gggtggaggc tgagtcaact atcaagggga agttagaaga
    26641 agcagggact tctactcgcc tatctcacct ggctcaagaa ggattgaaaa aagtagaaag
    26701 cacggtagga gaaaccctgg ctacagggga attatctcct ccaacttcgg gagttgaaaa
    26761 aagatctcct tttactaaaa gacctatcgc cgtaattgta gaaaatgccc cggcagcccg
    26821 tcctcaatcg ggtttaagca aagcagacat agtttatgaa atcttagccg agggaggaat
    26881 tactcgtttt ttagctattt ttaactctga accagcagaa aatgtaggtc cggtgcgtag
    26941 cgctcgtcct tattttatac ttaaagcggc ggaaaacgac gctatttttg ttcatagcgg
    27001 aggaagcgtg gaagcattta catacttaga aatgctggcg gtagaccata tcgatgagat
    27061 gaagaatttt caacctttct ggagaactca agaccgtcat cctcctcata atctctatac
    27121 ttctgtggct tctctgcgtc aggaagcgca gagattgggt tataataagc ccactcgagt
    27181 aggtggtttt ttggttggtg atttaggaaa tccaggagga gatagtgctc cgaggttaga
    27241 gattagctac actgccgatt ataaagttga gtttgtttat cttccagata aaaatgtcta
    27301 tcggcgcctt ataaatggtg cacctcatgt agatgcagca ggaggtcagg ctattcaatg
    27361 ttctaccatt attgttcagg tgactgaaca tcaagtaaaa gatgaagagg gaagattgga
    27421 aataaggttt gtaggagagg gcaccggctg ggtttttcga gatggcaaag tggtgcctat
    27481 tcgctgggag aaaagcgatc tccgggataa aaccaggttt tatcttgccg atggagaaga
    27541 gctaaaagtt aatccgggga aggtttggat agaggtagtt gacactcgca ctaaggtggt
    27601 cttttagatg tatccggtta tgtttcattt gggaagtttt cctgtttatt cttacggagt
    27661 gatgttagca gcagcgatcc ttgtgggtat tttttatgct attcgtcgtg cgcctcgcta
    27721 tcaggtgtct gctgatgcgg tggtggaagt tagcgcttta tgtattatag ggggggtcgt
    27781 aggagcgagg ttgctttatg ttttaataaa ctgggaatat taccgggaaa atttagctca
    27841 cattttttta gttcgagaag gagggctaac tttttatgga ggaatagctg gtgctctact
    27901 tttagttatt ccctatgttc attataaaaa atattctctt cctgtttttt ttgacatttt
    27961 ttctccaccc attgctttgg gttatgccat tgcccggata ggatgctttc taaatggttg
    28021 ttgttatggt cgagtgtgtc ctttcacttc ttttcctctg gcggtaaaat ttccctatct
    28081 ttccggattt agatatccta ctcagctata ttctgcgggt tattctctga taattctttt
    28141 tgctttattg catttagaaa aaaataaagc tttcactggt gagctttttc ttgattatct
    28201 gtggctttat ggtattgctc gttttctgat tgaatattta agagatgaac ccttttctgt
    28261 ggggggaatt tttactctgg ggcaggtggc atgcttatca atcgtagctt tttctttaat
    28321 tcttcgagag gttattaaaa gatatggaaa ggagtcttga tgcctgggta gaggcttttt
    28381 tagatttcct tttctgggaa aagagagtag cggataacac catacttggt tatggtcaag
    28441 acatatcatc atttgtccag tttttgaaag agaaagagat tgcctccttt ggggaagtaa
    28501 ctcttttagt tttggaggag tttgtgagtc aggaatcaga tagagagctg gaagctactt
    28561 ctttagctcg aaagatttct gctttacgca cttttttctc tttcctggtt cgagaaaaag
    28621 aagtaccaga gaatctggct cggcttctcg atacacctcg cttggaaaga aacctccccc
    28681 aggttttgag cctggaagag gtggaagctt tgctttccgc ttgcgataaa accaaacccc
    28741 ggggtttgag agatcgagca attcttgagc ttctttattc tagcggttta cgagtgggag
    28801 aggtggtttc tttagaattt tcccatctgg atttggaaaa tagaatgttg agactgtggg
    28861 gtaaaggatt taaagaaaga atggtccctt ttggagaaga agcccatgaa gctatctctc
    28921 tctatctgga gaagggaaga ggtgagattt tgaaaggtcg ctcgagcaat tatttattta
    28981 taaacggacc ttccgctcgt cccttgactc gccagagtgt ttggaatatg gtaaagaggt
    29041 acgccaagcg ggctaacttg cgtgaggata ttactcctca cactttgaga catacttttg
    29101 ctactcatct tctggaaaat ggtgctgatt taagagtggt acaggagttt ttggggcaca
    29161 gcgatatttc tactacgcag atttacactc atgtgagcaa aaaagttttg cgggaaacct
    29221 atgatcgggc ttttccccgg aaatagaaaa ggaggtaaat aacatggacg atatgaagag
    29281 aaaaattgaa gaaagttatc agtttatcaa ggaaagaatt gattttgttc ctcgagtggg
    29341 aattatactg ggaacaggct tgggggctct ggtggatgat atagaaattg agcgggtgat
    29401 accttatgag gaaatacccc attttccaat ttctactgta gagactcatc gaggaaatct
    29461 ggttttagga catttagagg gtaaaccggt ggtggtgatg cagggtagat ttcactatta
    29521 tgaaggatac gatatgaagc agataacttt tcctgtccgg gtgatgagaa agttaggagc
    29581 tgaaattctt ttggtaagca atgcagcagg agggatgaat cgctatatga aaagaggaga
    29641 tttgatgatt ataagagacc acatcaacct cctaggagat aatcctctcc gtggccccaa
    29701 tgacccggaa ttaggaccac gttttcctga tatgtctcag gcttatgata gagaactcgg
    29761 ggatcttgct ttgcaggtcg ctcttgataa tggaatttat gtacatcagg gagtatatgt
    29821 ggctttagct ggtccaaact tagaaactcc agctgagtat cgctttttga ttactattgg
    29881 agctgatgca gtaggtatgt ctactgttcc tgaggtaatc gtagctcgcc actcggggat
    29941 gagggttctg ggaatttctt gtattaccga tttggctatt gatggagtgg tggaagaagt
    30001 gagctttgaa gatatagtgg aggcagccaa tagtgctgaa cccaagctta ctttaattac
    30061 gagagaagta gtaaagagat taaccctcta atttatggta caagaactac tggaagtagc
    30121 gctgttggct gctttaccgg taagtgaggt ccggggagct ctcccttatg gggtattggt
    30181 gaggaattta cccttttcct gggtagtaat agtgtctttt gtttttaatc ttttgccctt
    30241 tttcctggtg atgtttgctc tgagtttctt tttgaaattt cttcttcgct ggaaatggtt
    30301 cagtaatttc tggtctaaat atataaccgg agcgcaaaat cggtttaaga actatgaaaa
    30361 atggggtaaa tgggggataa tgttatttgt gggtattccc cttcctttca ctggggtgtg
    30421 gactggatca gtagtttgct ttcttgttgg tctatcctgg agagattctt tacctttcat
    30481 attaggaggc ctgactatgg ctacaatact agtcagttta gcagtactgg gtggtaatta
    30541 tttgttcatg aggtaggtga tattatgctg aaagctgtgg aatggaaagg tgagactttt
    30601 caatttttag atcaaactct tttgcctctt gaagaggtat acatagaaac atctgattac
    30661 cgggaagtgg aggaagctat ccgccagtta aaaatccggg gagctcctct tctgggagta
    30721 acagcagctt atggcctttg tcttggagct ttggaaggag aaaaaggcaa ggttggagat
    30781 ttctttagct ttttagaaaa agtagatgtt gatttgcgca cctcccgccc tacagcagta
    30841 aatatctcct gggctttgga tcgcttgaag aagatctggg agcaggatat ggaggactgg
    30901 tctcctcgtc aggtagttaa ctccctcatt gatgaggcaa agaaaataga agaagaagat
    30961 tataaaaaca acgaaaacat cgcccgatat ggtgctcagc tccttcagga tgaagataga
    31021 gtgcttaccc attgcaatac cggatctctt gcctgtagtg ggtggggaac agctttagga
    31081 gcgattagct gggcggtgtt gcaagaaggg aaaaaaatcc aggtttacgc cgatgagacc
    31141 cgacctctgt ggcagggagc tcggcttact gcttttgagc ttatgaaaaa ttccatcccg
    31201 gttacggtga tatgtgataa catggctggt tacttgatgt ctcagggatt ggtagataaa
    31261 gtgatagtag gggcagaccg ggttactcta cagggcgatg cggctaacaa aattggcact
    31321 tattccttag ccgttctggc tcacta
//
LOCUS       DAOPSS010000004        30285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_1191_length_30285_cov_9.387694, whole genome shotgun sequence.
ACCESSION   DAOPSS010000004 DAOPSS010000000
VERSION     DAOPSS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 30285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30285
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1191_length_30285_cov_9.387694"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..274)
                     /locus_tag="PKN31_00650"
     CDS             complement(113..274)
                     /locus_tag="PKN31_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98467.1"
                     /translation="MMLELVDVWRNNLIFMMNMGITAENIKIRHYLLGRFLVSDCQEK
                     NYRRRGLRW"
     gene            complement(433..984)
                     /locus_tag="PKN31_00655"
     CDS             complement(433..984)
                     /locus_tag="PKN31_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA98468.1"
                     /translation="MKIIDLNKENKELIEQAATILYESFKDISEAWSTMELALGEVYE
                     SIGENRISRIAVNDDKVVLGWIGGKSMYNGNVWELHPLVVKREFRGQGIGKDLVNDLE
                     EKVRERGGLTIWVGADDENNSTSLSNTNLYENLYEKIVNIRNLKRHPFEFYQKMGFII
                     VGVMPDANGIGKPDIYMAKKVRK"
     gene            complement(1146..1328)
                     /locus_tag="PKN31_00660"
     CDS             complement(1146..1328)
                     /locus_tag="PKN31_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOA98469.1"
                     /translation="MQNYGRTLNTLRHSLATCLPKSGVDFIYIQELLGHKNSKINNRN
                     LYSMLVRKTLAKKSFR"
     gene            1908..2051
                     /locus_tag="PKN31_00665"
     CDS             1908..2051
                     /locus_tag="PKN31_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98470.1"
                     /translation="MKEITQYLVIKEQEELKRAKKLKEKFLRLILTGPGSPFRKQKKA
                     IGE"
     gene            complement(2038..3186)
                     /gene="wecB"
                     /locus_tag="PKN31_00670"
     CDS             complement(2038..3186)
                     /gene="wecB"
                     /locus_tag="PKN31_00670"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOA98471.1"
                     /translation="MKKVKTFFVLGTRPEVIKLAPVISSLSREKEFEIKIVNTGQHRD
                     MAGYFLQLFGLSPHYDLDVMAAKQSLFYLTSRIIKGLEGVFSQDRPDLVVVQGDTTSA
                     FCGALAAFYDKINCAHVEAGLRTREKYSPFPEEMNRVLISNLADLHFAPTPHSRKNLL
                     KEGIEEEKIYVTGNTIVDALFQVLEKNLPFQNEILLREIGEEENLKIITVTAHRRENW
                     EAGIEEVAKALRTLGERLPEIKIFFPLHPNPVVRDKIIPLLSTTSQVFLLEPLNYFDF
                     VKLLSRSTLLISDSGGVQEEASALGVPVLITRDFTERPEVIEAGLGYLVGCNQNEIVS
                     LATKLLEEREERKSRLVFGDGQASSRIVQILRRFLKLDFSPFEEFTPR"
     gene            complement(3173..4225)
                     /locus_tag="PKN31_00675"
     CDS             complement(3173..4225)
                     /locus_tag="PKN31_00675"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOA98472.1"
                     /translation="MKQVLFFSFLSCISAVVVVPFVIHFSEKKKWLDMPDGKRKVHVK
                     PVPHLGGIALFLAFFLAVFFLSLLGYFSFPFYLLAGGGVIFLTGLIDDLLFLPPWGKL
                     IGQTLGALILIAGGVTIQFFTLPWDTMIYLGNWGYPLTLLWIVGITNALNLIDGMDGL
                     SAGIGAIASFTLGIIAWQEGRIEPAVLSFLLTGATVGFLFYNFPPAKIFLGDSGALFL
                     GGMLATISVQGAVKNAATFTLAIPILILGVPILDTFFAIIRRSKNHLPVSSADRGHLH
                     HRLLERGYSPRRVVVLFYIISIFLGVIAIIVNTFVRNGTYSLLLLLLLFALFWRWGKS
                     LGVFDIPEGKKTVEKS"
     gene            complement(4222..4686)
                     /locus_tag="PKN31_00680"
     CDS             complement(4222..4686)
                     /locus_tag="PKN31_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOA98473.1"
                     /translation="MKDRPDWDTYFMSIAHLVSTRSTCLRRQVGAVMVRDKQILTTGY
                     NGVPRGITHCTPENCLRSVKDIPSGQQQELCRGLHAEQNAIIQAALHGVSTKDATLYC
                     THKPCILCAKMLINAGVVRIVYQDFYPDPLADEMLEEAGIEMCIWEGRVISR"
     gene            complement(4676..5122)
                     /gene="rpiB"
                     /locus_tag="PKN31_00685"
     CDS             complement(4676..5122)
                     /gene="rpiB"
                     /locus_tag="PKN31_00685"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOA98474.1"
                     /translation="MKLLIGSDHGGFELKEDLKIFIQELGYEVKDYGTYDIQSCDYPD
                     VAFQVAEDVAKGEGERGILICGTGIGMSIAANKVKGIRASLCHDTFSARFAREHNDAN
                     ILTLGGRVVGKGLAREIVRIWLEANFTGERHARRLNKIEEYEKNER"
     gene            complement(5103..5984)
                     /gene="prmC"
                     /locus_tag="PKN31_00690"
     CDS             complement(5103..5984)
                     /gene="prmC"
                     /locus_tag="PKN31_00690"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOA98475.1"
                     /translation="MPSVKEVWQGLVKDLKEHSFSSPAREARLLLASVLEVEPSRVYL
                     FWDEELPPPKLTHLHDIFKKRLQGVPIQYLAGEWEFFSLPFRLRKGVFIPRLDTESWV
                     EEAIVYLRSLPEEKIMVCDMGCGSGVIGISCAFWVENLYLYGADISPKAVELSKENAH
                     LLGVEKRSVFLLSDLFSAFPPVKFDVILSNPPYVREEDWDSLSPEIRLYEPREALLAG
                     EEGLDMIERVLKEGESFLKEGGLFFLEHDPEQKEAIQKLADTLFLEYLRSIPDLSGRE
                     RASVIRKRRSKNETAYR"
     gene            complement(5985..7055)
                     /gene="prfA"
                     /locus_tag="PKN31_00695"
     CDS             complement(5985..7055)
                     /gene="prfA"
                     /locus_tag="PKN31_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOA98476.1"
                     /translation="MWREKIGEIVKEYQDLTQKIANPQSMKDMATYRSLTQALAEIEP
                     VVGKYEEYEKKERELEENRELLRVESDLELRALLEEEIEKLKTSLQELEEELKVMLIP
                     RDPRDEKNTLVEIRAGTGGEEAALFVADLFRMYSHYAERNGWKVEVMSSNPTELGGFK
                     EIIFAVEGKGAYSKFKFESGVHRVQRVPVTEAGGRIHTSTATVAVLPEADEVDVEIRP
                     EDIRVDVYRSSGPGGQSVNTTDSAVRVTHIPTGMVVTCQDEKSQHKNKAKALRILRSR
                     LLEMERESQKEEIDQERRIQIGSGERSERIRTYNFPQNRVTDHRINLTLYHLESVLEG
                     ELDEIIEALAAEEKMRTLEKVS"
     gene            complement(7024..7875)
                     /locus_tag="PKN31_00700"
     CDS             complement(7024..7875)
                     /locus_tag="PKN31_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOA98477.1"
                     /translation="MMRSPFRLATAVRKPNGDIALEVKEQRPIAQKYPFLSWPIIRGV
                     VGLIDSLVVGIKALSHSASLALEEEEEKLSSMDIGISILLALGIFAGLFIALPAFLSS
                     LVDQFISSTFVYNLVEGLIRIGIFLAYLLLISQMKDIRRVFEYHGAEHKAIFTWENGE
                     ELTPENARKFTTLHPRCGTNWLIIVLVISIFVFSFLGRPGLVTRIVSRVLLIPLVAGL
                     AYEAIKILSRYQDNRLAYILTLPGLLLQRITTREPDDSQLEVAFAALKGSLGEDNYVA
                     GENRRDS"
     gene            complement(7919..8581)
                     /gene="thyX"
                     /locus_tag="PKN31_00705"
     CDS             complement(7919..8581)
                     /gene="thyX"
                     /locus_tag="PKN31_00705"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOA98478.1"
                     /translation="MKVTLLSYTPDPEITVALAARTCYSSKLPLAISKERAEHLIREL
                     IERGHESVLEHASFTFAIEGISRVTSHQLVRHRLASYSQQSQRYVSVKEADFIIPPSV
                     VENSEAQELFCKAIEESKKAYQELINLGIPQEDARYMMAQGIATNLVLSANARELLHI
                     FRLRLCNRAQWEIRELAQKMLELVKDLAPAIFEWAGPPCVEGECREGENSCGKPWKKR
                     EK"
     gene            complement(8631..8837)
                     /gene="rpmE"
                     /locus_tag="PKN31_00710"
     CDS             complement(8631..8837)
                     /gene="rpmE"
                     /locus_tag="PKN31_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOA98479.1"
                     /translation="MKKGIHPEYKETRVICACGNTFVTRSTRFPEIKVEICSKCHPFF
                     TGQQKLVDTAGRVEKFRNKYGDNY"
     gene            9898..10980
                     /locus_tag="PKN31_00715"
     CDS             9898..10980
                     /locus_tag="PKN31_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOA98480.1"
                     /translation="MFKIVSVNGGDMNPKEKKSFTIQDIAKLTGFSIGTVDRALHHRE
                     GIKPETREKILEVAEQVGYRTNRVASALSRKKPLVFAAVFPRELHYFYDDVRRGFQDA
                     IERLKDFRVAPLYKDIETLGSGEEEALSSLLSQEISGVVFAPGHSHRFNHLINNFTQR
                     GIPVVAVSTDAPESKRLTTVCVNPRKNGELAGELMANWVKGEVAILIGSQEIDDHYQK
                     TIGFQEALAQFQPSPQLVAILESQESENRAEENTRKLLKNHPELSGIYIATANSVGVC
                     RTLEESGRAQEIKVIATDLFPEVVHYLKKGVINATIFQNPYRQGFEATMSLFQFLVEG
                     IAPPAYLYLEPVIVMRSNLEFYFPAH"
     gene            10991..12328
                     /locus_tag="PKN31_00720"
     CDS             10991..12328
                     /locus_tag="PKN31_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOA98481.1"
                     /translation="MRKLTIISMIFIVLVFGFSLGYATELAVWSSPDNADALFELAQN
                     FMQAHPEIQIEVTPLSWEVLYPRILQDITAGVGAFDVTTWDLMTAGAIAPGMLDLEAF
                     AQEHPDLVDPDFDDDDFIPTARYIYGYWGDKRIGQPFYGAAMFFFYRQDLFQDKALQE
                     KFQAQYGRELRVPETWQEAKEVAQFFTKKFNPDSPTEYGIGLMFPRTHTLFYMFLNFF
                     GPYRRSPEGIEKFGEVDLDWGDLFTADHQPAFNSEEGVKAIQDMIDLLPYSSDPLGSD
                     YGETLEAFSKGMVAMVPQWTACMASWQESPDLQPFEEKVGIAIMPGKAPVSGGWGVGI
                     NAFSKNQEAAFQFIQYATSKEGDKIQWLKYRVGPTRKSVIEDPEVIADAPWLKDVYLP
                     SLEGASHRPRIPEEPRVEDVLVGTISEILLGQAPNSIETLNLVAEEWKKVLSK"
     gene            12370..13254
                     /locus_tag="PKN31_00725"
     CDS             12370..13254
                     /locus_tag="PKN31_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOA98482.1"
                     /translation="MMNSKKRKRTGFLLILPLLILFLGLTIYPLIYMIYMSFHQYSLA
                     SWEEPRFIGWENYREILRDRTASSSVSFTLFLLFTAVPLEILLGIGIAFLIRDVWGER
                     ALRSSLIIPMTIPPVVAGIAWKMLYNYEFGPINYFLSLFGLPKVSWLGSQFFARLGVL
                     LIDVWQWTPFIFLVIYAGLQSIPRDVVEASLVDGASGWSAMRYIELPLLRPLIWVTLI
                     IRVIDVLKLFDIVYMVTFGGPGSATHSYSFYIYKVGISFGWDVGYASALSVILLLVVT
                     VLTNLLIRFLRLSETLGL"
     gene            13266..14114
                     /locus_tag="PKN31_00730"
     CDS             13266..14114
                     /locus_tag="PKN31_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOA98483.1"
                     /translation="MKWLTKTIKFILIGMVIFFFLFPVYWLVTTAFKRSEDWFTWPPS
                     FFPQHWTLGNFLGLEGGFFGSVTTAISTITPYLINSIVIALSVALISTIIAALAAYAI
                     SRFKVGGMGFVSWIISIRMLPPIAAALPLYVIFSHLHLVDTWWALILSHLIFTTPFST
                     WILITFFNEIPQELDEAAFVDGANIGQTFWHITLPLSAPGLASVSTLAFIQSWGEFLM
                     ALVLTTSKNAQTLPIYLGRFITGWRIAWGPLAAAGLVTMLPVVVFSLLMQRYLLQGLT
                     FGAVKG"
     gene            complement(14118..15539)
                     /locus_tag="PKN31_00735"
     CDS             complement(14118..15539)
                     /locus_tag="PKN31_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA98484.1"
                     /translation="MKFFKSVKFRLTVWYLIVMGILLFIFAALSYFTLYHNLYENLDN
                     NLEDRLIKLRSLLRDAGEDVDIDIDMERELGEIILIYYPEGFLWKSSGPFIDAPDITP
                     MVREVAKGAPLFFNTTTTYGRVIRFYAAPITVGERSFILLVGRYFDEIKSILGRLRTI
                     FILSGLVMMVLAGVGGLFLADRALRPVDRITRTAQKIGERNLSHRIEVHSEDELGRLA
                     LTLNQMIARLEDAFRHQQQFTADASHELRTPLSVIQAEATLALGKQRREKEYREALET
                     ISQEASYMSSIIDKLLFLARSDIRREQYNFEEVNLRELVIDLATSIEALAQEKGLEFE
                     VGTVKDLVVRGDKDRLRQLLFNILENAIKYTKTGKVSISAVQKDKMAVVAIADTGAGI
                     PSQDLPHIFERFYRVDKTRSREEGGTGLGLAIAQEIAEAHGGKIEVESELNKGSTFSV
                     TLPISPVASSSGKKKTKEKHRKK"
     gene            complement(15536..16207)
                     /locus_tag="PKN31_00740"
     CDS             complement(15536..16207)
                     /locus_tag="PKN31_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA98485.1"
                     /translation="MRILVVEDEKRLAEVIKKGLVEEGYSVDVAYDGAEGQYMAESAS
                     YDLVILDIMLPKKDGVTICQELRIRGINTPILMLTAKDSVEDRVKGLDSGADDYLVKP
                     FAFSELVARVRALLRREALSKASKLQVGNLVMDTLTREVWRGDKKIELTAKEYAILEY
                     FMRHPNMVVTRTMLMEKVWDYDFEGISNVIDVYIRRLRLKLDEEGAESIIETVRGAGY
                     RLREP"
     gene            16343..16999
                     /locus_tag="PKN31_00745"
     CDS             16343..16999
                     /locus_tag="PKN31_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOA98486.1"
                     /translation="MKGNRYLVGILVALLLSVTFSVAACAQEAIPEQPIAPEEAPAGE
                     QPIAPEEQPMMPEEPPAPERFPMDEAIKAALTVFPEAQAVSAKFSDPLFGDAVYLIEL
                     SNGMEVKVDAQTGEVLGSTELSEPSPAVTLLPQTTVSLEEAVRVALSTKQDRAAVEAK
                     LVEEESNIVYVIKLDNGEEIRVDAALTEEAPAEEPTTEVEEQSEEAPAEETPVEEPVF
                     "
     gene            complement(17292..17435)
                     /locus_tag="PKN31_00750"
     CDS             complement(17292..17435)
                     /locus_tag="PKN31_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98487.1"
                     /translation="MCLSGRRESGFWTTLVWLISKSYANMAGEELVSDTKDAQRLIEF
                     LAS"
     gene            complement(17516..17662)
                     /locus_tag="PKN31_00755"
     CDS             complement(17516..17662)
                     /locus_tag="PKN31_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98488.1"
                     /translation="MATKKQIESAKKNIKKTQKIWKETSHRQRALAQPQGRDRAKMGK
                     TRGR"
     gene            18057..18422
                     /locus_tag="PKN31_00760"
     CDS             18057..18422
                     /locus_tag="PKN31_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98489.1"
                     /translation="MNKRRLNYTLSLVVVLSFLLVLGLSGCTTLTSGEKDFSGPTRGF
                     GEDSPNTFPLYAGQTIPVGEVQVWNDDENVCVKYQLSDDAIAEGWLIYETHLAVGATL
                     GDIPQTKKRQSYSGTIPLR"
     gene            18430..19578
                     /locus_tag="PKN31_00765"
     CDS             18430..19578
                     /locus_tag="PKN31_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98490.1"
                     /translation="MDGVESYQECVPLPAGVSCDDDIIIAAHAVVEKTECEIITEAPY
                     GGLKVVNYAQGLRYDYTSVRSERSNPNAVLAFEEGHNESYFFSLGFNEDRSGYSDVDA
                     WIIVEFDKPIQNGTGNDLRVVEDTWGLPYPDETADVWVSKNGTDWKYLGEADNQHPMS
                     SYHTITDFDLDDVGLDWAKFVKVQNTSVRDDFARLYPGQGSTLDGYDLNAILALQNYQ
                     ECTTYSETAWGAGDRFVEQGNWATYITYTVECEEPTPACSYETAWGGDTEGGSGKGWW
                     YYFDPSKGLTQTIWAGQIINVGNVTVSGCSDGKATITITLTDGWELQDVDKDGKPVTE
                     PVKIQGYDEIPDERPPAGKFETYKGNSPTVEVPCFNYYAIHLDVQLCE"
     gene            20395..21870
                     /locus_tag="PKN31_00770"
     CDS             20395..21870
                     /locus_tag="PKN31_00770"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOA98491.1"
                     /translation="MSKLIDNSYKLFIDGKWVDGKEGKVYQEYNPANGELLATCVEAG
                     KEDVDMAVNAAWRAFETWKDTSPQERAAMLLKIADLIDENAEKLAMIETLDNGKPIRE
                     TSTIDVPLSSDHFRYFASAIRAAEGEAVMIDKDTMSIILREPIGVVGQIVPWNFPLLM
                     AAWKLAPAIAAGDCVVLKPSSETSLSTLELAKLMSEVLPPGVVNIVTGRGSTTGNYML
                     EHEGFRKLAFTGSTEVGYTVAEAAAKKLIPATLELGGKSANIYFEDAPWDKAMEGLLI
                     GILFNQGQVCCAGSRVFVHDSIYDKFLSDAVAAFEKVKVGLPWEKDTQMGSQINEAQL
                     KKILSYIEIGKKEGAKVATGGYRITDHGLDKGYFMKPTILVDVDNKMRVAQEEIFGPV
                     VCFIRFKDEDEVIKMANDTEYGLAGAVWTKDINRALRVARGIEAGRIWVNDYNNLPAH
                     APFGGYKKSGIGRETHKVVLEHYTQMKNIFISLTEEKAGLY"
     gene            21903..22112
                     /locus_tag="PKN31_00775"
     CDS             21903..22112
                     /locus_tag="PKN31_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98492.1"
                     /translation="MITKDTPVEEIMQKYDVLAYFLENGISPFSCAGAFPQSLGKLLE
                     IKKVKDPDAFIAGLNAWIDEQERAL"
     gene            complement(22189..24060)
                     /locus_tag="PKN31_00780"
     CDS             complement(22189..24060)
                     /locus_tag="PKN31_00780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA98493.1"
                     /translation="MKDLDTVPRRLLAVLIPAWNEAEVIGPMVENLFCSINYPSSLFH
                     IFIGVYPNDSATLREVQILEERYPNVHGVVNPQPGPTSKAQNLNQVLKFILRFENQKR
                     LRFACFLIHDAEDIIHPTSFKLANYLTFQHEVVQLPVFPLQHYPTWRTFFKFITSSTY
                     ADEFAENHYRGLLAREASGAFVPSAGTGFIISHSVLDKMGSQDIFEEDSITEDYKLSF
                     RLGELGIPTRFFLEGVERVLDNGKVTTEYIATREIFPNTLREAVKQKSRWIYGISFQS
                     FSFREVIKDKNFPPIFKYSLYRDWKAKYANLLSIPGYAVFAYFILSLFYVLPPVYPVR
                     SLSWWLSVVLTFLMVERQVMRGIALKQVYGWRSAIAGCLIPPLLPLRAVWGNIINFLA
                     TVSAWRICFFGFPKNRPRWQKTKHSYLPPEVLLRYQRKLGDLALEKQIVAPSVLAELL
                     REAKEKGEKIGEVLIREGAISQEELNSILAEVLKSGYIELDNHSVRITEIAPEGLEKF
                     KKYCVMPVAITKHSVILAAGAPLPLEAQQEIKESLGGRNLSVVLSSPSLIAQALQSLE
                     EPDSLPQFPRLGEKLLDRGLINEEQLVEALRAQKFYPKPLGEILVEMGLVRPEDLEN"
     gene            complement(24212..25777)
                     /locus_tag="PKN31_00785"
     CDS             complement(24212..25777)
                     /locus_tag="PKN31_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA98494.1"
                     /translation="MNEVGERIEITPQFSETLELLENTSRSVFITGKAGTGKSTLLRI
                     FRDNTKKKVVVLAPTGVAALNVRGQTIHSFFRFKPDITQDKVKKITKNKDKNIYQEVD
                     TIVIDEISMVRADLLDCVDKFLRLNSGKAKEPFGGKQMVFIGDLYQLPPVVKAKEREI
                     FKTWYDTPYFFSAHVFQNLPFEFIELEKIFRQKDEIFIEILNAIRNGTATEELLAVLN
                     QRYKPDYVPPEGEFYIYLTTLNRLANEINEEQLARIPGLTYDLTGTIEGDFDERDLPT
                     EKTISLKIGAQVMLLNNDPYGQWVNGSVGKVVEIEKDKSGEVKTVVVELRDGEEVYVE
                     PFTWEIFEYQYDEKSASIKTHTLGSFTQFPLRLAWAITIHKSQGKTFDRIILDISGGT
                     FSPGQVYVALSRCTTLEGIVLKQPLEKRHIFMDWKVPRFLSEYQYRIAEEKMPQEEKI
                     HLLQEAVEEKKSLEIVYLKGNNEKSRRIIDPLYVGELDYEGKKFTGLQAYCHKRKEIR
                     NFRIDRILDIRKL"
     gene            complement(25770..27242)
                     /gene="gltX"
                     /locus_tag="PKN31_00790"
     CDS             complement(25770..27242)
                     /gene="gltX"
                     /locus_tag="PKN31_00790"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOA98495.1"
                     /translation="MAEVRTRFAPSPTGFLHLGGARTALFNWAFARKEGGKFILRIED
                     TDVARSTEEAVEVILDSLRWLGLHWDEGPYFQSQRLEIYRKYALLLWEKGLAYECFCT
                     PEELKERRQETMAQGESWRYDRKCWNLKEEEKEKLKKEGRKSILRFYIPPGETTFIDM
                     LRGEVHFHNEELDDFVIMKSDGMPTYNFACVVDDALMKISHIIRGDDHISNTPRQVLL
                     YQAFNFPIPQFAHIPMILGKDKTRLSKRHGSPSVTYYRDQGYLPDALFNYLARLSWAS
                     GEEEKEIFSREEIVERFSIENVTKHAAVFDLDKLNWMNSVYIRQADNKYLCSILEEIL
                     VRRGRIKPEEITPSHREYWEKVIALMKERIKYVAQLEEEASYFFTEEVEFDPVAFEEV
                     LRREGVEEILKECEEVLSRIEDFRAPVLEEAVREMAKKRGVKAAQIIHPLRVAVSGKK
                     VGPGLFELLEVLGKERVIGRIQRARQQIKEGYPLRDSGDE"
     gene            complement(27235..27645)
                     /locus_tag="PKN31_00795"
     CDS             complement(27235..27645)
                     /locus_tag="PKN31_00795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOA98496.1"
                     /translation="MNFIDDDNCLFCGSQNPYGFHLVFRQEDDFTVTETSVPWYYQGF
                     KGVVHGGIVAALLDEAMSHSLKNAGIEAVTGTLETRYIKPLLTEKPLVARGRVVKKNG
                     RIIEVQGEVIQEGEVVARGKGAFIVPRERSEKIG"
     gene            complement(27642..28736)
                     /locus_tag="PKN31_00800"
     CDS             complement(27642..28736)
                     /locus_tag="PKN31_00800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOA98497.1"
                     /translation="MRKPWFRKPALWVILFLLIWSLFLLWGEWKKGEEVSNHYLTLAR
                     LYPQNEERYLALFLLEEIKKDAPLEVVKTPEEGGGWEVQVTLTNQESREEFSRKMLDL
                     FQLLSSFGFVVKQEKEGGKDFYSIFRGAEPWFFLETFIPRRAQIALVIDDLGYNLEMA
                     ERFLDLPVKLNVAIFPHLPLAKKIASLAEEKGKEILIHFPMEAEDDRENSGEAFLLRV
                     GEREEEVREKVEEVLRLIPQAKGINNHKGSRATSDYLLMSHFMESLKGRGVYFLDSLT
                     SSHSLAYRLASQKGIPSFCRDVFLDGELSLSYVITQLQKTVEIAKKNGQAIAIGHPNE
                     VTYEAIYQFVSNFSEPGVEIVFLSEMEERN"
     gene            complement(28714..29886)
                     /locus_tag="PKN31_00805"
     CDS             complement(28714..29886)
                     /locus_tag="PKN31_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOA98498.1"
                     /translation="MRDKRIWLIGCLIALLALIVAWESWENKGAYGLGGTIDLEEWQP
                     FFETLLLLQESFYSETPLEGRALMEEAIRGVLRATGDPYARYLNEEDYKVETSDRVEG
                     EFSGLGIVIAIKDDKLTVISPFQGSPAEKAGIKAGDVIVEIDGVPALGMSSDEAVRRM
                     RGERGTSVTLTIQREGEEENIVVEVVRDIIKIQSVEYEMLSGSIGLIRISEFHGRTQE
                     ELKKALSSLQVAGVEGLILDLRNNPGGLLQSAVLASGMFVPRGNPLLIVEDREGKRES
                     ITNFVDPVWKGPIVLVINKGTASGAEILAGVLRVHLGAELVGETTFGKGVVQEIFPLS
                     HGGGVIFTVSKYLLPDGTDLNGKGLEPDILVSEEEEQIKKAEEELRRLIDEKALVS"
     gene            complement(29897..>30285)
                     /locus_tag="PKN31_00810"
     CDS             complement(29897..>30285)
                     /locus_tag="PKN31_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOA98499.1"
                     /translation="GRLFWPVQGTVVEGFGERKDPRYGISFYNPGIDIGAEKGSPVFA
                     AHDGVVILAQTLRGYGKTILLDHGDIITMYAHLSEIKVEGGQKVKGGEVIGAVGDTGL
                     VERPLLHFEVRVGSSAREEDPLKWLR"
BASE COUNT         8605 a         7023 c         5785 g         8872 t
ORIGIN      
        1 ttttatgaga ataaaactac ccctttttag gtgacatttt cacagactat tgacaattga
       61 caagaaaagc agataggttc gagctttctt cctattaaaa tggatagccc tctcaccacc
      121 tcagccctct cctccggtag ttcttttcct gacaatcgct cactaagaaa cgccccagga
      181 gataatgcct tatttttata ttttctgccg ttattcccat attcatcata aaaatcagat
      241 tatttcgcca aacatccacc aattctaaca tcaccgaata ttagtcgtca tgtggattta
      301 agagaactcc tgcttgattt ttcaccctct ttgagcccaa aacctgaagt aggaatgaag
      361 ctgtgctccg cagtataacc cggggttaca aaagatagta acctgtttct ttcttgatct
      421 acctgcaaat attcattttc ttactttttt ggccatgtaa atatcaggtt ttcctattcc
      481 atttgcatct ggcattaccc ctacaataat aaaacccatt ttctggtaaa attcaaatgg
      541 gtgccttttt aaattcctaa tattaactat tttttcataa agattttcgt ataaattcgt
      601 atttgaaagg gacgtagaat tattctcatc atcagctcct acccaaattg ttaacccacc
      661 acgttccctg accttctctt ctaagtcatt caccaggtct tttcctatac cttgacccct
      721 gaactctctc ttaactacaa gcggatgaag ctcccaaaca tttccgttat acatgctttt
      781 accgccaatc catcccagga cgactttatc atcattaact gcaattctac ttatcctatt
      841 ttctccaatt gattcataca cttctcccag ggctaactcc atagtcgacc atgcttcgga
      901 tatatcctta aaactttcat ataaaattgt agcagcctgt tctatcaatt ctttattttc
      961 tttgtttaaa tcaattattt tcatcttctc acccccaatt tttgctttta ccttataatc
     1021 ggaatcggcc aggaagggcc acgtccaatg agccaggcta tatccgatta tggatgaagt
     1081 gccagaattt tcccttctta ataaattttg tcaccaggtg cctcttccgc tcaggcttaa
     1141 accattcatc taaaagactt ttttgccaga gttttccgca ctaacattga gtataaattt
     1201 ctgttattta tcttactatt tttatgacct aataattctt gaatgtatat gaaatcgact
     1261 ccgctttttg gtaaacacgt tgcaaggctg tgccttaatg tattaagtgt tctcccgtaa
     1321 ttttgcaggt atgctcaaag attttctgaa ccgctctgac atgttgagat tattttgaag
     1381 ctccttaact ccattcaggg ctcctgcgtt aacacttgta gaaaaaccgg ctacataaca
     1441 ggatctgttt agattttccg gtctctaaat ttccattagt atttgaaaat gatcagtatt
     1501 tatcaacagg atatcagcga tgttctttga cggtttttat cttgtttaca aaaacaagat
     1561 cgtaagggaa caaagaccca gtcttgcctg actggctttt ttaatatcga cttctgacga
     1621 tggtttattt ttctctggat ttttcacttg ttgcagcaaa ttataaacga tattcataga
     1681 gtttatcaaa cctccaagtt gtctcgtgtg gctattctgt aataatgtca tcttcaaaaa
     1741 ttctcggaga aattccttta gtaataaaat gaccgcagaa agataagaaa atgggcacat
     1801 ccgcaatatt atgatgcagc tctaaaagct gatagcacaa tgacagtgaa tgagaaaaga
     1861 ttaggcaaga atatccagta aatatggggg tgcatcctca caagataatg aaagaaataa
     1921 ctcaatattt agtaataaaa gagcaagaag agctgaaaag ggctaaaaaa ctcaaagaaa
     1981 aatttctgag attgattttg actggaccgg ggagcccatt cagaaaacag aaaaaagcta
     2041 tcggggagta aactcttcaa agggagaaaa atccagtttc aggaagcgcc gcaaaatctg
     2101 gacgatccgt gaggaagcct gtccatcacc aaaaaccaac cggctcttcc tctcttctct
     2161 ttcctccaaa agcttggtgg ctaaagatac gatttcattc tggttacaac ctaccagata
     2221 cccgagaccc gcctctatca cttccggcct ttcggtaaag tctcgagtaa ttaaaactgg
     2281 tactcccaga gccgaagctt cctcctgcac cccccctgaa tcactgatta aaagagtgct
     2341 ccgggaaaga agcttgacaa aatcaaaata attcagcggt tctaagagaa aaacctgaga
     2401 ggtggtagag agaagaggga taattttatc cctcaccact ggattgggat gaagaggaaa
     2461 aaaaattttt atctctggta atctttctcc cagggttcgg agagctttcg cgacctcttc
     2521 tatccctgct tcccagtttt ctcgacgatg agcagtgact gttattattt ttaaattttc
     2581 ctcctctcct atctcccgga gaagaatttc attctggaaa ggcaggtttt tctctaaaac
     2641 ctggaaaaga gcgtccacga tagtgttacc ggtaacgtag attttttcct cctctattcc
     2701 ttccttgagg agatttttcc gagagtgagg agtaggtgca aagtgtaaat cagcaagatt
     2761 gctgattaaa acgcggttca tctcttccgg gaaaggactg tacttttctc tggtacgcaa
     2821 gcccgcctct acatgagcgc agtttatttt atcgtaaaaa gcagcaagag ctccacaaaa
     2881 agcgctggta gtgtctcctt gtactacgac taaatctggc ctatcttgag agaaaactcc
     2941 ctctaaccct tttataatcc gggaggtaag ataaaaaaga gattgtttag cagccataac
     3001 atctaaatca tagtgaggag aaagtccaaa aagttgaaga aaatagccag ccatatcccg
     3061 atgttgaccg gtatttacga ttttaatctc aaattctttt tcccgggaaa gagaagaaat
     3121 aactggagcc agctttataa cctcagggcg agtacccaga acaaaaaaag ttttaacttt
     3181 tttcaacagt ttttttccct tctggtatat caaacactcc caggctcttc ccccatcgcc
     3241 agaagagagc aaaaagaagg agcagaagga gcagagagta agtaccattt cgaacaaaag
     3301 tattgacaat gatagctatt acccccaaaa agatgcttat tatatagaac aaaaccacta
     3361 cccttcgggg ggagtacccc cgctctaaaa gacgatgatg gaggtgtccc cgatcagcag
     3421 aagataccgg taagtgattc ttactcctcc taatgatagc aaagaaagta tccagaatag
     3481 gaacccctaa aatcaaaatg ggaatagcca gagtgaaagt agcagcattt ttaactgctc
     3541 cctgcaccga tatggtagcc agcattcccc ctaaaaatag tgcaccacta tctcccagaa
     3601 aaatcttcgc tggaggaaaa ttgtagaata aaaatccaac agtagctccc gtaagaagaa
     3661 aagataaaac cgctggctct attcttccct cctgccaggc aatgattccc agggtaaaag
     3721 aagcaatagc tcctatacca gcggataagc catccatacc atcgataaga tttaaagcgt
     3781 tagttatccc tactatccag agtaatgtca gagggtaacc ccaattgccc agataaatca
     3841 ttgtatccca gggaagggta aaaaactgaa tggtaacccc tcctgcaata aggataagag
     3901 cacctaaggt ttgtcctatg agcttccccc agggaggtag gaatagaagg tcatctatta
     3961 aaccggtgag aaatataact ccccctcctg ccagaaggta aaagggaaaa gagaagtaac
     4021 ccagaagaga gagaaaaaaa acagctaaaa agaaagcaag aaataaagca attcctccca
     4081 ggtgaggaac aggtttaacg tggaccttcc tcttcccatc cggcatatct aaccattttt
     4141 tcttttcact gaagtggata acgaagggaa ctacgaccac agccgaaata caagatagaa
     4201 aactaaaaaa cagaacctgt ttcaacggga tatcacccgc ccctcccaga tgcacatttc
     4261 tattcctgct tcttctaaca tttcgtcagc taaaggatcg ggatagaaat cttgatatac
     4321 tatcctcact actccagcgt taattagcat tttagcacat aaaatgcaag gtttgtgagt
     4381 gcaatacaga gtagcgtctt tagtgcttac tccatgtaag gctgcctgta ttatagcatt
     4441 ctgctcagcg tgtaatcctc tacacaattc ttgctgttga ccagaaggaa tatctttcac
     4501 gctgcgtaaa cagttctccg gggtacagtg ggttatccct cggggaactc cattataccc
     4561 ggtagtcaag atttgtttgt ctctcaccat aactgcccct acctggcggc gcaggcaggt
     4621 agagcgagta ctcacaagat gagcaataga cataaaatag gtatcccaat ccggtctatc
     4681 tttcattctt ctcgtattcc tctattttat tcaaccggcg agcatgcctt tctccagtaa
     4741 aattagcctc taaccatatc cggacgattt cccgagccag gcccttcccc accacccttc
     4801 ctcctaaggt gagaatattg gcatcattgt gttcccgggc aaaacgggcg gaaaaagtat
     4861 catggcatag tgaagccctt attcctttaa ctttattggc agcaatggac ataccaatac
     4921 cggttccgca gattagaatc cctctttctc cctccccttt tgctacatct tctgccacct
     4981 gaaaagctac atcaggataa tcacaagatt gaatatcgta ggtcccgtaa tcttttacct
     5041 catatcccag ctcctgaata aaaatcttga ggtcttcttt cagctcaaaa cctccatggt
     5101 cgctacctat aagcagtttc atttttactc ctccttttcc gaattacact ggctctttct
     5161 ctccctgaaa ggtcaggaat agaacgtaga tattccagaa aaagagtatc agctaatttt
     5221 tgtatggctt ctttttgctc cgggtcatgt tctaagaaaa ataacccccc ttcttttaag
     5281 aaagactccc cttctttcaa aactctctct atcatatcta gcccttcttc tccagcaaga
     5341 agtgcctccc gaggttcata gagtctaatt tcgggagata gagaatccca gtcctcctct
     5401 ctgacatagg gggggttaga aagaataacg tcaaacttaa cagggggaaa agcagaaaaa
     5461 aggtcagata gaagaaaaac gcttcttttc tccactccta aaaggtgagc attttccttt
     5521 gatagctcca ctgctttcgg ggaaatatct gctccatata aatagaggtt ttctacccag
     5581 aaagcacagg atatgccaat tactccactc ccgcagccca tatcgcaaac cattattttt
     5641 tcttccggta aggagcgaag gtaaacaata gcttcttcca cccagctttc tgtatctaaa
     5701 cgagggataa acactccttt tcttaagcga aaaggaagag agaagaattc ccattccccg
     5761 gcaaggtact ggatgggaac gccttgcaag cgtttcttga aaatatcgtg caggtgagtt
     5821 aattttgggg ggggaagctc ctcatcccag aaaagataaa ctcgagaagg ctctacttct
     5881 aaaacagagg ctaaaagcaa gcgagcctct ctcgcaggag aagaaaaaga gtgttctttt
     5941 aagtctttta ctaagccttg ccacacctct ttaactgagg gcatttaact cactttttct
     6001 agcgttctca ttttttcttc tgccgctaaa gcctcaatta tttcatccag ttctccttcc
     6061 agaactgact ccagatgata aagagttaag ttaattcggt ggtcagtaac ccgattttga
     6121 ggaaagttat aagtgcggat tctttcgctt cgctccccgc taccaatttg tattcttctt
     6181 tcctggtcta tttcttcttt ctggctttct ctttccattt ccagtaaacg gctacgcaaa
     6241 attctcaaag ccttagcttt atttttatgc tgggattttt catcctggca agttactacc
     6301 attcctgtgg ggatatgggt aacccgaaca gcagagtcag tagtattgac gctttgtcct
     6361 cccggacctg aggaacgata cacgtctacc cgtatgtctt ccggcctgat ttctacatct
     6421 acctcatcag cttccggaag tactgccaca gtagccgtag aggtgtgaat acgccctcct
     6481 gcctcagtta ccgggactct ctgcacccga tgcactccac tttcaaattt aaatttacta
     6541 taagctcctt ttccttctac agcaaatatt atttccttaa atccccccag ctccgttggg
     6601 ttagagctca ttacttctac tttccaacca ttgcgctctg cataatgact gtacattcgg
     6661 aaaagatcag ctacaaataa ggctgcttct tcccctcctg tgccagctcg aatttccacc
     6721 agggtgtttt tttcatctcg ggggtcccgg gggatgagca ttactttgag ttcttcttcc
     6781 aattcttgaa gggaagtttt taacttctct atttcttctt ctaaaagagc acggagctcc
     6841 aggtcacttt ctacacggag aagctcccga ttctcttcca gctctctttc ttttttctca
     6901 tattcttcgt acttcccgac tacaggctct atctcagcca gagcttgagt taagctccgg
     6961 taagtagcca tatccttcat cgattgagga ttggctattt tttgagtcaa atcttgatat
     7021 tccttaacta tctctcctat tttctcccgc cacataatta tcctccccta aactcccttt
     7081 caaagcagca aaagccacct ccagctgact atcatccggc tctcgagtgg tgatgcgctg
     7141 cagaagtaaa ccgggcaaag ttaaaatata agctaagcga ttgtcttgat accgggacaa
     7201 aattttaatc gcctcataag ccagacctgc tactaaagga ataagcaata ccctgcttac
     7261 tatcctcgta actaaccctg gcctacccag aaaagagaaa acaaatatac tgattaccag
     7321 cactataatt aaccagttgg ttccacagcg aggatgaagg gtagtaaact ttcgagcatt
     7381 ttctggagta agctcctcac cgttttccca agtaaaaata gctttatgct ctgctccatg
     7441 atattcgaac actcgtctga tgtctttcat ctgggatatt aataataaat aggctaaaaa
     7501 aatccctata cgaattaatc cctccaccag gttataaacg aaagtagaag atataaactg
     7561 atcaactaaa gaagaaagaa aggcaggcaa agctataaac aaaccggcaa aaattcccag
     7621 agccaaaagg atagaaatcc cgatatccat actgctcaat ttctcttctt cctcctcgag
     7681 tgccagcgaa gcagagtgag aaagggcttt gatacccact accaaagaat caattaatcc
     7741 caccactcct ctaattatag gccaggataa gaaaggatat ttctgagcaa taggtctttg
     7801 ctcttttacc tctaatgcaa tatcaccgtt aggtttgcgc accgccgtgg ctaaacgaaa
     7861 aggactgcgc atcatcaccc cctcgatgag agcctgtcct cctacatcga atttcacttt
     7921 atttttctct ttttttccag ggttttccac aactattctc tccctctcga cactctccct
     7981 ctacacaagg aggtccagcc cattcaaaaa ttgctggagc caaatctttt actaattcta
     8041 acattttttg ggccaattct cgaatttccc actgagctct attgcacagc cgcaggcgaa
     8101 agatgtgcaa tagctcccga gcattagccg acagaaccag gttagtagct ataccctgcg
     8161 ccatcatata acgggcgtct tcttgaggta ttcccaaatt tatcagttcc tggtaagctt
     8221 ttttactttc ctctatagct ttacaaaata gttcttgagc ctctgaattc tcaacaacag
     8281 aagggggaat tataaaatca gcttctttta cagagacata acgctggctc tgttgagaat
     8341 aagaagccag acgatgcctc accagttgat gagaagttac ccgtgatata ccttcaatgg
     8401 caaaagtaaa agaagcatgc tctaacactg attcatggcc tcgttcgatg agttcccgaa
     8461 tcaggtgttc cgctcgctcc tttgagatag caagaggaag cttgctgcta taacaagtac
     8521 gagcagctaa ggcaacggta atctcaggat caggtgtata actcagaaga gttactttca
     8581 tcatcgcaga atgatgtagc tagaaaagat agctacctga ggaagcgaaa ttaataatta
     8641 tctccatatt tattacggaa tttctctact ctccctgcag tatctactaa cttctgttgt
     8701 ccggtaaaga aaggatggca tttagagcaa atttccacct tgatttcagg gaatctggtg
     8761 gaacgggtaa caaaagtgtt accgcaggca caaattactc gagtttcttt atattcggga
     8821 tgaattcctt ttttcatggc tttatatcac ctctacttta gagtcacacc attatagcaa
     8881 atgcttattc tctctgtcaa aaaccactgg tagaaaattg cttccgtcta tttttttcct
     8941 tttttataat atcccgcaaa gcatgctaaa ccactcaaga tttgaaagcc ggttacgttg
     9001 ggtaaaaaat tcggatcatc tcagtgaggg atacttttta ttcctcagtt ttcccctcag
     9061 ctaacttttg ttttttattc tgcctttatt ttttacgtct tacacctacg tcgtaaactg
     9121 tctatttcca tgactgaaaa agatgctttt cttcctccgt agattttgct ttttacattt
     9181 tttgtttttt tatattattg tctttcggag gattataaaa ggaagctttt cttccctatt
     9241 atagctcaga gtccgtcatt cctgaaattt agcgggaaac ctgccttctc tgccatgcct
     9301 gaagtctttt atcaagcatc caaatattct tgaatactgt ggacctctcg cagagacatt
     9361 caggggtgac agacaatggg atgataaaac cattcagggg cagactaaaa acggctcgaa
     9421 agttgccggg gtggcgaaca aaaaggtgat aggaccattc aggggtagca aaaaaagaaa
     9481 ttaggttata agggatcggt ttccccttat tgccgggttt ataaggaggt ctttttttat
     9541 actgggaaag aaaaaatgtc tttcccggta gttcttgctt ttttatttca tccttctcag
     9601 cgaaagagaa tctcactccc aaccttttat ttcttcttcc cgactggctc aattctttcc
     9661 ctgctgtttc cttagaaagc catggaatct gtatctttta gagagaatgg aaaataactt
     9721 ccactcctct tcacaactta tatgtcgctc aaactgcgag aattttcact ctcgtcttgc
     9781 gggtaaaagt cctacttcgt taaaaacaaa cttacaagaa tttttcagat gacattatca
     9841 cagaataact acaggagcct tataaaagag tagaaaataa tcttgattaa tgttacaatg
     9901 tttaaaatcg ttagcgttaa cggtggggat atgaatccta aagaaaaaaa atctttcact
     9961 atccaggata tcgccaaact aactggtttt tccatcggaa cagtggaccg ggctcttcat
    10021 catcgagagg ggattaaacc cgaaaccagg gaaaaaattt tagaagtagc agagcaagtt
    10081 ggttatcgaa ccaatcgagt agctagcgcc ctgagccgga aaaagcccct ggtttttgct
    10141 gctgtttttc ctcgagaact ccactacttt tatgatgacg tacgtcgagg ttttcaagat
    10201 gctatagaga gattaaaaga cttccgggtt gctcctcttt acaaagatat agaaactctg
    10261 ggaagcggag aagaagaagc tctaagttcc ctgctttccc aggaaatatc gggagtagtc
    10321 tttgctcccg gtcactccca caggttcaat cacctcatca acaacttcac ccagcgcggt
    10381 atacctgtag ttgccgtttc tactgatgct ccggaaagta aaagattgac caccgtttgt
    10441 gttaatcctc gcaaaaatgg tgagttagct ggagagctga tggctaactg ggtaaaagga
    10501 gaggtagcta ttttaatagg ttctcaagaa atagatgacc actatcaaaa gacaataggc
    10561 ttccaagaag ctttagccca attccagcct tcacctcaac tagtagcaat tttagaaagc
    10621 caggaaagcg aaaacagagc ggaagaaaat accagaaaac tcttaaaaaa tcatcccgaa
    10681 ctttctggta tttatatcgc taccgccaac tcagtagggg tctgtcgtac cttagaggaa
    10741 agtggtcggg ctcaagagat aaaagtaatc gctaccgacc ttttcccaga agtagttcac
    10801 taccttaaaa agggggtgat aaatgctact attttccaga atccttatcg gcagggtttc
    10861 gaggctacta tgtctctttt tcagtttttg gtggaaggaa tagctcctcc ggcttatctt
    10921 tatttagagc cggtaatagt aatgagaagt aatttagagt tttatttccc agctcattaa
    10981 ggaggaaaaa atgagaaagt taactataat aagcatgatt tttattgttt tggtttttgg
    11041 tttttctctg gggtatgcaa cagaattagc agtttggtct tctccagata atgctgacgc
    11101 cctatttgaa ttagctcaaa actttatgca ggcacacccc gagatacaga ttgaagtcac
    11161 tcccctttcc tgggaagttc tatatccccg catcctgcaa gatatcactg ctggtgtagg
    11221 agccttcgat gtaaccacct gggatttaat gaccgccggc gctatagctc cagggatgct
    11281 ggatttagaa gcttttgctc aggaacatcc tgatttagta gatccagatt ttgatgatga
    11341 cgattttata cctaccgccc gctatatcta tggctactgg ggagataaaa gaattggtca
    11401 acctttctac ggagcagcaa tgttcttctt ttatcgccag gaccttttcc aggataaagc
    11461 cctgcaagag aaattccagg ctcagtatgg tagagaactc cgagttccag aaacctggca
    11521 agaagctaaa gaagtggctc aatttttcac taagaagttt aaccctgatt ctcccactga
    11581 atacggaata ggtttaatgt tccctcgcac ccatactctt ttctatatgt ttctcaattt
    11641 ctttgggcct taccgacgca gcccggaagg tattgaaaag ttcggggaag tagatttaga
    11701 ttggggagat ttattcactg ctgatcatca accagctttc aattctgaag aaggagtaaa
    11761 agccattcaa gatatgatag acctccttcc ctactcctcc gatcctttag gctctgacta
    11821 tggagagacg ttagaagctt tcagtaaagg gatggtagcc atggtgccgc agtggactgc
    11881 ttgcatggcc agctggcagg aatcccccga tcttcagcct tttgaagaaa aagtgggaat
    11941 tgctataatg ccaggtaaag caccggttag tggaggttgg ggagtaggaa ttaatgcttt
    12001 ttctaaaaat caagaagcag cttttcagtt tatccagtac gccacctcta aggagggaga
    12061 caaaattcag tggctaaaat atcgagtagg ccccactcgt aaatcggtta tcgaagatcc
    12121 cgaagtaatt gccgacgctc cctggctgaa agatgtctat cttccttctt tagagggagc
    12181 ttctcaccga ccccgcatcc cggaagaacc tcgcgtggaa gatgtgctgg taggaactat
    12241 atcggagatt cttttgggac aagcacctaa tagcattgaa actttaaatt tagtagcaga
    12301 agagtggaaa aaagttttga gtaaataaaa agtagggggg tctcccccct actttaagag
    12361 gggaaaacta tgatgaactc taaaaagaga aagcgaactg gttttctatt gattcttcct
    12421 cttttgattc tatttcttgg cttaaccatc tatcctctca tttatatgat atatatgtct
    12481 tttcatcaat attccctggc ttcctgggag gaacccaggt ttattggatg ggaaaattac
    12541 cgagagattc taagagaccg taccgcctcc tcgagtgtta gctttacttt atttttactt
    12601 ttcaccgctg tacctcttga aatccttctg ggaattggca tagcttttct catccgggac
    12661 gtatggggag agcgagctct acggagctct ctaattattc ccatgactat tccaccggta
    12721 gtggctggta tagcctggaa aatgctctac aactatgaat ttggccctat aaattatttt
    12781 ctctctcttt tcggccttcc taaagtctcc tggctgggaa gtcagttttt tgcccgcctg
    12841 ggggtacttc tgattgatgt gtggcagtgg actcccttta tatttttagt aatttatgct
    12901 ggattgcaat ctattccccg ggacgtagta gaggcaagtc tcgtagatgg agccagtggc
    12961 tggtcagcca tgcgctatat tgagttacct cttttgcgcc cccttatctg ggtaacactc
    13021 attatccggg taatcgatgt tctaaaactt tttgatatcg tttatatggt aacttttggt
    13081 ggcccggggt cagccactca ttcttacagt ttttacattt acaaagtggg gatatctttc
    13141 ggctgggatg tagggtacgc ttctgctttg agtgtcattc ttcttttagt agttactgtt
    13201 ttaactaatc tgctcattcg ttttttgcgt ttatcagaaa ctttagggct ttaggaggaa
    13261 aaaaaatgaa gtggttaact aaaacaatca agtttatcct catcggaatg gtgattttct
    13321 tttttctttt cccggtatac tggttagtta ccacagcttt taaaagaagt gaagattggt
    13381 tcacctggcc tccttccttt ttcccccagc attggaccct gggtaacttc ttaggattag
    13441 aaggaggatt ttttggtagt gttaccactg ctatctctac tattactcct tatcttatca
    13501 atagcatcgt tatagcgcta tctgtcgctt taatttctac catcattgcc gctttggcag
    13561 cctatgccat ttctcgcttc aaagtgggag ggatggggtt tgtctcctgg atcatttcca
    13621 ttcgcatgct tccccccatt gctgctgctc tgccccttta cgttattttc agtcatctac
    13681 acctggttga tacctggtgg gcgctcattt tatctcattt aatatttacc actcctttca
    13741 gtacctggat tctcatcacc tttttcaatg aaattcctca agagctggac gaagctgctt
    13801 ttgtggatgg agcaaatatc ggacaaactt tctggcacat cactctacct ctcagtgctc
    13861 cgggactcgc ttcggtatcc actttagctt ttatccagag ctggggagag tttctcatgg
    13921 ctctggttct caccaccagt aaaaatgctc agactcttcc catttatttg ggtagattta
    13981 taaccgggtg gcggatagcc tgggggccac ttgctgcagc aggattagta accatgcttc
    14041 cagtagtcgt gttttccctt ctcatgcagc gctatctctt gcaaggtctc acttttggag
    14101 cagtgaaggg ctaaagctta ctttttgcga tgtttctctt tagttttctt tttacctgaa
    14161 ctcgaagcaa ctggagagat aggtaacgtc actgaaaaag tgcttccctt attcaattcg
    14221 ctttctactt caatttttcc cccatgggct tcagcaattt cctgggcaat ggccaaccct
    14281 aaacctgttc ctccttcttc tcgagaacga gtcttatcta cccgatagaa gcgctcaaaa
    14341 atatgaggca ggtcttgaga gggaatacca gcaccagtat cagcaatggc caccactgcc
    14401 attttatctt tttgaacagc tgagatagag actttccccg tcttggtata cttgatagca
    14461 ttctccagaa tattaaacaa aagctgtctt aacctatctt tatctcctct taccaccagg
    14521 tcttttaccg tccccacctc gaattccagc cctttttcct gggcaagcgc ttctatactg
    14581 gttgctaaat cgataaccag ctctcggagg ttaacttctt caaaattgta ttgttcccgt
    14641 ctaatgtcgc tacgagctaa aaaaagcaat ttgtcaatta ttgatgacat ataagaagct
    14701 tcttgagaaa ttgtctccaa agcttccctg tattctttct ctcttctctg tttaccgaga
    14761 gccagagtgg cttctgcttg aataacagat aagggggtgc gcaattcatg agaagcatcg
    14821 gcagtaaatt gctgctgatg gcgaaaagca tcttctaagc gagctatcat ctggtttaga
    14881 gtaagagcta accttcccag ctcatcttca ctatgtactt ctatccgatg acttaaattt
    14941 ctctctccta tcttttgagc agtgcgggtt attcgatcta ccgggcgaag tgctcggtcg
    15001 gccaaaaaca atcctccaac accagccaaa accatcatta ctaaaccaga gaggatgaag
    15061 atggtacgca atctacccaa aatacttttt atttcatcaa aataacgacc cactaataaa
    15121 ataaaagatc gctcacctac cgtaatcggc gcagcataga atcggattac tcgaccatag
    15181 gtagtggtgg tattaaaaaa aagaggggct cctttggcta cttccctcac catcggagta
    15241 atatcaggcg catcaataaa aggcccggag ctcttccaca ggaaaccttc aggataataa
    15301 attaatatga tttctcctaa ctctctttcc atgtcgatat cgatatccac atcttctcca
    15361 gcatctcgta gtaaactccg taatttaata agtcgatcct ctaaattgtt gtctaaattc
    15421 tcatacaagt tgtgataaag agtaaaataa gataaagcgg caaaaataaa aagtaaaata
    15481 cccataacta tgagatacca aacggtcaaa cgaaatttaa cacttttaaa aaatttcatg
    15541 gttctcttaa cctgtaacca gctcccctta ccgtttcaat gatactctct gccccttcct
    15601 catccagctt aagtcgcagc cgacgaatat agacatctat gacattagat ataccttcaa
    15661 aatcataatc ccatactttt tccataagca tggttcttgt aactaccatg ttaggatgac
    15721 gcataaagta ctccaaaatt gcatattcct tggcagttaa ttcgattttt ttatctcctc
    15781 gccacacctc tctggttaag gtatccatca ccaaattgcc cacctgaagt tttgaagctt
    15841 tagaaagagc ttctcgccgc aaaagcgctc ttaccctggc tacgagttca ctaaaagcaa
    15901 aaggtttcac cagataatca tcagctcctg agtccagacc ttttacccta tcctctacgc
    15961 tgtctttcgc tgttagcatc aatatggggg tattaattcc cctgatgcgt agctcctggc
    16021 aaatagtaac tccatctttt ttgggcaaca taatatcgag tattacgagg tcataggaag
    16081 cactttcagc catatattgc ccttctgctc catcataggc tacatcaacc gaatagcctt
    16141 cctccaccag gcctttttta ataacttcag ctaagcgttt ttcgtcttcc accactaaaa
    16201 tacgcatttc atttttatta tactgaaatt tttcattttt catctcaaat taatgttgaa
    16261 ttcatcacct tttcattttt cgggattata ttatcctcag acgaggaaaa acccgtcaaa
    16321 ataaaacaga gaggtgataa gaatgaaagg gaaccgttac ctcgtgggaa ttttggtggc
    16381 actgttactc agtgtgacat tcagtgtggc agcttgtgct caggaagcta taccagagca
    16441 acccatagca ccagaggaag cacctgcagg agagcaacct attgctccgg aagaacaacc
    16501 tatgatgccg gaggaaccac ctgcaccaga gcgctttcct atggatgaag ccataaaagc
    16561 agctttaact gttttccctg aagcgcaagc agtatccgcc aagtttagtg atccactttt
    16621 tggcgacgcg gtgtatttaa ttgaactcag caatggaatg gaagtcaaag tagatgctca
    16681 aactggggag gtgttgggta gcacagaatt atctgagccc agcccagcag ttactcttct
    16741 accccaaacc acagtttcct tagaggaagc agtaagagta gcattaagca ccaagcaaga
    16801 tagagctgca gtggaggcaa aattggtaga agaagaaagt aatatagtct atgtcattaa
    16861 actggataat ggagaagaaa taagagtaga cgcagcctta actgaagagg ctccagctga
    16921 ggagcctacc acagaagtcg aggaacaatc tgaggaggct ccagctgagg aaactccagt
    16981 tgaggagcct gttttttgaa aaaaataagc tcctccctaa atcaatcctc cgaggaaggg
    17041 ccgggcggcc cttcctccct ttttaaactt ttcctatcca gatatcatat caatcaaaag
    17101 aaaaatcgag tttaggagta aagctcccta tttcataata gattttttac ttattcttag
    17161 tttttgtttt tagccttgct gcttgcgctt ttttaatgct ttctctggtc tctatcgagc
    17221 gggcgtgggt tttctttttc tggaacattt ttcctatcga gctttggaca tacgccttct
    17281 acgtattcag attaagaagc taaaaattct atgagtcttt gagcatcttt ggtatcggaa
    17341 accagctctt cccctgccat gttagcataa cttttactga tgagccacac gagagtagtc
    17401 cagaaaccac tttctcttct tccagaaagg cacaagatgt aacctttttc tcccccatca
    17461 tagtagcaaa aaaaacagtg agcgcttccc gaggtttacg atgatgcaga agcactcacc
    17521 ttcccctggt tttccccatt ttagctcggt ctcttccttg tggctgggct aaagcgcgct
    17581 ggcgatgcga cgtttccttc cagattttct gggtcttctt gatgttcttt ttggctgact
    17641 caatttgttt cttggtagcc attttcttcc ctccttaaat tgaaaaactt actcttaccg
    17701 ataatattat aaatctacgc taaacaaaaa tctaatttac cttcttcatc cgatcttcag
    17761 ctcccaaagc ggatagtaag cctaaaacaa gtttccgcta ataaactctt aataaaaagg
    17821 cggggatggg cagagaaagc ctcactaccc gcaggaatta atagctcggt aaagctttat
    17881 cagtatggga agtttaaaat gaagggaagt tttacctgcc caggtccgga tatccctcct
    17941 ataaattagt actattttcc tattaagatt ggctactttt cctattttaa ttttttttag
    18001 tgatgagaga atatgaatat gaaggtgggg aggaatctcc cagaggagga acggacatga
    18061 ataaaagaag attaaattac acgctgtctt tggtagtggt attaagtttt ttactggttt
    18121 taggtttatc gggatgtacc actttaacat cgggtgagaa ggatttttct ggtcctacac
    18181 ggggttttgg tgaggattca ccaaacacat ttcctctcta tgccggtcag actataccgg
    18241 tcggagaagt acaggtctgg aatgatgacg aaaatgtgtg tgtgaaatat cagcttagcg
    18301 acgatgccat tgcagagggt tggctcattt acgaaacgca tttagcagtt ggtgctactc
    18361 taggtgatat tccccagaca aaaaaaaggc aatcctattc cgggacaatt cccttacggt
    18421 gatgatgact tggatggtgt agaatcttac caggaatgtg tacctctgcc tgctggtgta
    18481 agctgtgatg atgatattat tattgctgcc catgcggtgg tcgaaaagac agaatgtgaa
    18541 ataataaccg aagctcctta tggcggactt aaggttgtta actatgcgca gggattacgt
    18601 tatgactata catctgtcag atcagaacgc tccaatccta acgcagtatt agcttttgaa
    18661 gaagggcata atgaaagcta cttcttcagt ttgggattta atgaagatcg ctctggttat
    18721 tctgatgttg atgcctggat tatagttgaa tttgataaac caattcaaaa cggaactggt
    18781 aatgatctac gggtagttga agatacctgg ggtcttccat atcctgatga aactgctgat
    18841 gtttgggtaa gcaaaaatgg tacagattgg aaatacctgg gagaagcaga caaccaacat
    18901 ccaatgagtt catatcacac gataactgat tttgatctgg atgatgtggg actggattgg
    18961 gcaaaatttg tcaaggttca gaatacatct gtcagagatg actttgcacg tctctatcca
    19021 ggacagggaa gtacactgga tggttatgat ctcaacgcaa tccttgcatt gcaaaactat
    19081 caggaatgca ctacttatag cgaaactgcc tggggtgctg gagatcgctt tgtcgaacaa
    19141 ggaaactggg cgacctacat cacctatact gttgaatgcg aagaaccaac acctgcttgc
    19201 agctatgaaa ctgcctgggg tggggacaca gaaggcggca gtggcaaagg gtggtggtat
    19261 tactttgatc cttctaaggg gttaacccaa accatctggg ctggacagat tatcaacgta
    19321 ggtaatgtca cagtctctgg atgtagtgat ggaaaggcaa ctattactat tacccttact
    19381 gatggctggg aattacagga tgttgataag gatggtaagc ctgtcacaga accggtcaaa
    19441 atacaggggt atgatgagat tccagatgaa aggccaccag ctggaaagtt tgaaacctac
    19501 aaaggtaatt cacctactgt agaagttccc tgttttaact attatgctat tcacctggat
    19561 gttcagctct gtgagtaaca aaacctgctg tcaggggacg gttctttgac agattgagaa
    19621 ttgaaccaaa gaccgttctc gacagggtga gatgacctga taataaaagg cgacagatta
    19681 ttttttatca tcttctcgtt tttttccggt ctccctcccg cccttattat ggattccaat
    19741 cccggttctt cagctatttt aatctacctg aattcccatt atgaaaacct ccggcaatca
    19801 agccgcttcc tgatgttatc aaaggtttaa ggtctttatt gcggtaaatc ttaagtacag
    19861 tgcgacccgt aagtctgtac tttggtgaat attaccaagg attcaagcaa tagctgaact
    19921 cgtgtaacaa aatatccaca tcctcacctt aactctcctc ggttatacat caatccccgt
    19981 tggtaaaaat ttcgtttctt tctcaagaca gcatacacaa tcttaacata ttgcctagcc
    20041 aagcatgaaa gagtgtttcg atgggctttc ccttccttcc ttttcttcct gagtgaggaa
    20101 aggagaaacc tttcctcact tcctttgtct tgacaagaag atcaaatccc tttccctctt
    20161 taaagcgcta cccccttccc tgaatttccc tgcgaaaagt agattgcaag gtgactatcc
    20221 cttcccccga agacacagag catagttcct tgcttgattt ttcatatcaa taaggatatt
    20281 atggaaccaa gaaggggggt gcttatatgt gcatttagcc acagcgttat taacctccgg
    20341 tgatgcatgg gaaaagccga cattttttaa aaaacacaaa ggaggttttc tcacatgtct
    20401 aagctgattg ataatagtta taagcttttt attgatggca agtgggttga tggcaaagaa
    20461 ggtaaagtat accaggaata taacccggct aacggtgaat tgcttgccac ctgtgtagag
    20521 gcggggaaag aagatgttga catggcagta aatgcagcct ggagggcatt tgaaacctgg
    20581 aaagatacga gcccccagga gcgtgcagct atgttgctga aaatagctga cttaatcgat
    20641 gagaatgcag aaaaattagc catgatagag actctcgaca atggtaagcc aatacgtgaa
    20701 acttctacta tagacgttcc cttgagttca gaccattttc gttattttgc atcagccata
    20761 cgagcggcag aaggtgaagc ggtgatgatt gataaagaca ctatgagtat cattttaaga
    20821 gagccaatag gtgtcgtagg tcaaatagta ccctggaatt tcccgcttct catggccgca
    20881 tggaagctgg ctcccgctat agcagccggc gattgtgtgg tgctcaaacc ttcttctgag
    20941 acatctttga gcacgttaga gcttgccaag ctcatgagtg aggttttgcc ccccggcgtt
    21001 gtaaacatcg tcactggccg aggttccacc accggaaatt acatgctcga acatgaaggt
    21061 ttccgtaaat tagcttttac aggctccaca gaagtcggct acaccgtggc tgaagcagcg
    21121 gccaaaaagc ttatccctgc tacacttgag ctgggtggca agtcagctaa tatttacttt
    21181 gaagatgcac catgggataa agctatggaa ggcctgttga taggcatttt atttaaccag
    21241 ggacaggtct gttgtgcagg ctcccgggtg tttgtccacg attcaatcta tgataaattc
    21301 ctctctgatg ccgtggcagc ttttgaaaag gtaaaagtag gtcttccatg ggaaaaagac
    21361 actcaaatgg gctcgcaaat caacgaagcc cagctcaaaa aaattctctc ctatatagag
    21421 ataggtaaaa aagaaggggc taaagtagcc accggtggtt accgtatcac cgaccatggt
    21481 ctcgacaaag gctatttcat gaagcccacc attttagtag acgtagataa caagatgaga
    21541 gtagctcaag aagaaatctt cgggccggtg gtctgtttca tccgcttcaa agatgaagat
    21601 gaggtcatca agatggctaa tgataccgaa tatggtcttg ctggtgcggt gtggactaaa
    21661 gatatcaacc gggccttacg cgtagccaga ggcatcgaag ctggtagaat atgggtgaat
    21721 gattataata atttgcccgc ccatgcccca ttcggcggtt acaaaaaatc cggaatcgga
    21781 cgcgagacac acaaggtcgt tcttgaacat tatacccaaa tgaaaaatat ctttatcagc
    21841 ctcactgaag aaaaagcagg actctactga atacataata gctaataaag gtagtgattg
    21901 taatgattac taaagatacc ccggtcgagg aaattatgca aaagtacgac gttcttgctt
    21961 atttcctgga aaatgggatc tctccattta gctgtgccgg tgctttccct caaagtttgg
    22021 gcaaattgct tgaaattaaa aaagtaaaag accctgatgc ttttatagca ggacttaatg
    22081 cgtggatcga cgaacaagag cgggctttat aaagcccgct cttttatgac aatatagggc
    22141 agataacttt ttcgtttttt tctacctcgg ttgaatcaag aaaaacagtc agttctccag
    22201 gtcttctggc ctcactaaac ccatttctac caatatttct cccaaaggtt tgggatagaa
    22261 cttctgggct ctcagggctt ctaccaattg ttcttcattt attaatcccc tatctagaag
    22321 cttttctcct aaccgaggaa attgaggtaa agagtctggt tcttccaggc tttgaagtgc
    22381 ttgagcaata agagaaggac tggatagaac tacggagaga ttccttcctc ctaaactttc
    22441 ttttatttcc tgttgagctt ccaaaggaag aggagctccg gcagctaaaa ttactgaatg
    22501 cttggttatc gctactggca taacacaata ctttttaaac ttctcgagcc cctcgggagc
    22561 aatttctgtt attctaaccg agtgattatc cagctctata tagccacttt tcagaacctc
    22621 tgccaaaatt gagttcagtt cttcttgaga aatagctcct tctctgatta aaacttcccc
    22681 tattttttct cctttttcct tcgcttccct gagaagttca gctaaaactg atggagctac
    22741 aatttgcttt tccaatgcta aatctcctaa tttccgctgg tagcgcagta gaacctcggg
    22801 aggaagataa gaatgctttg ttttctgcca ccggggacgg tttttgggaa aaccaaagaa
    22861 gcatatcctc caggcagaaa cagtagccag aaaatttatg atatttcccc ataccgctcg
    22921 cagaggcaaa agaggaggaa tcaggcaacc agctattgcc gagcgccatc cataaacttg
    22981 cttcagggct atcccccgca tcacttgccg ctcaaccatt aaaaaagtga gcaccacgct
    23041 caaccaccag gataaagacc gcaccgggta aacaggaggt aaaacgtaaa acagggagag
    23101 aataaaataa gcaaagactg catatccagg aatagataaa agattcgcat atttcgcctt
    23161 ccaatcccgg taaaggctat atttaaaaat gggaggaaaa tttttatcct tgataacttc
    23221 cctaaaagaa aaactctgga aagaaatacc ataaatccag cgtgacttct gtttgacagc
    23281 ctcccgaaga gtattgggga aaatttctcg agtagctata tattctgtgg tcactttccc
    23341 gttatccagc accctctcaa cgccttccag aaaaaaacgg gtgggaatac ccagctcgcc
    23401 caagcgaaag gatagtttat aatcctcagt tatactatct tcttcaaata tatcctgact
    23461 tcccatttta tctaacacag agtgagaaat tataaaccct gtccctgctg agggaacaaa
    23521 agccccgctt gcctctcgag ctaaaagccc ccgatagtgg ttttcagcaa attcatcagc
    23581 ataagttgaa gaagtaataa acttaaagaa agtacgccag gtagggtagt gctgaagagg
    23641 gaaaactggc agctgcacca cctcatgttg aaaagttaaa taattggcca gcttaaaaga
    23701 agtagggtgt attatatctt cagcatcatg gatcagaaaa caagcaaaac gaaggcgttt
    23761 ctgattttcg aaacggagta taaatttcaa aacctggttt aaattttgag ctttactggt
    23821 tggtcccggc tgggggttca ctaccccatg cacattagga tacctctcct cgagaatttg
    23881 cacctccctc aaagtggctg agtcattggg ataaacacca atgaaaatat gaaaaagcga
    23941 ggaaggatag ttaatggaac aaaataaatt ctccaccata ggaccgataa cttctgcttc
    24001 attccaggcc gggattaaaa ccgccaataa acgccgggga acagtgtcta aatctttcat
    24061 ctgcagatga tttccggctc ttttccgccg ggaaaaataa taacaagcat cccagaagag
    24121 gtcatccaga ctaaaaaaga tgaatagaaa agcaatgata aatcccagat aataaatcgt
    24181 cgtagccaga gaaatagttt actccaatcg cttatagttt tctgatatct agaatccggt
    24241 ctatccggaa gtttcttatt tctttccttt tgtggcaata agcctgaaga ccggtgaatt
    24301 tttttccttc atagtccagc tctcccacat aaagagggtc aattatccgt ctcgattttt
    24361 cattgtttcc cttgaggtaa acaatctcca gactcttttt ctcctccaca gcctcctgca
    24421 aaagatggat cttttcctcc tggggcattt tctcttctgc aattcgatat tgatattcgc
    24481 tcaggaaacg gggtactttc caatccataa agatatgcct tttctcaaga ggctgcttga
    24541 gcacaatccc ctccagagtg gtgcaccggc tcaaagctac ataaacctgg ccaggtgaaa
    24601 aagtaccccc ggatatgtct aaaattattc ggtcaaaagt tttaccctgg cttttgtgaa
    24661 tggtaatagc ccaggcaaga cgcagaggaa actgggtaaa ggagcctaaa gtatgggttt
    24721 taatcgaagc tgatttttca tcatattgat attcaaatat ctcccaggta aaaggctcaa
    24781 catatacctc ttccccatct ctcaactcta ccaccactgt tttcacttcc ccggatttat
    24841 ccttttcaat ttccaccact tttcccacac ttccgtttac ccactgacca tatgggtcat
    24901 tgttcaggag cataacttgc gcccctattt tcagagagat tgtcttctct gtaggaaggt
    24961 ctcgctcatc aaaatctcct tctatcgttc cggtaaggtc ataagttaaa ccggggattc
    25021 tggccaattg ttcctcattt atctcattag ctaaacgatt gagagtggtc agatagatat
    25081 aaaattctcc ttccgggggt acatagtcag gcttatatct ctggttaaga acggccagaa
    25141 gctcttcagt cgcggttccg ttacgaatgg cattcaaaat ctcaataaat atttcatctt
    25201 tctggcggaa tattttttcc agctctatga actcaaaagg aagattttga aaaacatgag
    25261 ccgagaaaaa ataaggcgta tcataccaag ttttaaaaat ctccctttct tttgctttaa
    25321 ccaccggggg aagttgataa aggtcgccaa taaaaaccat ctgttttcca ccaaaaggct
    25381 ctttcgcctt accgctatta agacgcaaaa atttatccac acaatccaag aggtcagctc
    25441 ttaccataga aatttcatca ataacgatgg tatccacttc ctgatagata tttttatcct
    25501 tatttttggt aatctttttt actttatcct gagttatatc cggtttgaat cggaaaaaag
    25561 aatggatggt ttgtcctcta acattcaaag cagcaacccc ggtaggagcc agaaccacta
    25621 ctttcttttt agtattatca cgaaaaatcc gcagaagagt ggatttaccc gtccccgctt
    25681 ttccggtaat aaaaacacta cgtgaggtat tttctaaaag ctcgagagtt tctgaaaact
    25741 gaggggtgat ttcaattcgc tctcccacct cattcatcac cactatcccg gagagggtaa
    25801 ccttccttaa tctgttgcct ggctctctgg attctcccga tcactctttc ttttcccaat
    25861 acctcaagga gctcaaaaag ccctggcccc acttttttcc ccgataccgc tactcgcaga
    25921 ggatgaataa tttgtgccgc tttcactcct cgctttttcg ccatttctct caccgcttcc
    25981 tcgagaactg gcgctcgaaa atcctctatt cgagacaaca cttcctcaca ttcttttaat
    26041 atttcttcta ctccttctcg ccgtaaaacc tcctcaaaag caacggggtc aaactctacc
    26101 tcttcagtga agaagtaact ggcttcttct tcaagctgag ccacatattt aattctctct
    26161 ttcatcaggg ctattacttt ttcccagtat tctctatgag aaggggtgat ttcttctggc
    26221 tttattctcc ccctgcgcac cagaatttct tctaaaatag aacacagata cttattatct
    26281 gcctggcgaa tatagacact attcatccag tttagcttat ccagatcaaa aaccgctgcg
    26341 tgtttggtaa cattttctat agaaaatctc tccactatct cttccctgga gaatatttct
    26401 ttttcctctt ctccactggc ccagcttaaa cgagctaaat aattgaaaag agcatcagga
    26461 agataaccct gatctcgata ataggtaacc gaaggcgaac cgtgtctttt actcaaacga
    26521 gttttatctt tgcctaaaat catgggaata tgagcaaatt gaggaatggg gaagttaaaa
    26581 gcttgataaa gtaaaacttg ccggggagtg ttggaaatat ggtcgtcacc ccggattatg
    26641 tgagaaattt tcattagggc atcatctacc acgcaagcaa aattataggt gggcattcca
    26701 tcggatttca taatcacaaa gtcatctaat tcttcattat ggaagtggac ttctcctcgc
    26761 aacatatcta tgaaagtagt ctctcccgga gggatataga agcgtaaaat tgactttctc
    26821 ccttcctttt taagtttttc cttttcttct tcttttaggt tccagcattt gcggtcatag
    26881 cgccagctct caccctgagc catagtttct tgtcttctct cttttaactc ttccggagta
    26941 cagaagcact cataagccaa acctttttcc cagagcaaaa gagcatattt tcgataaatt
    27001 tccagacgct ggctttgaaa atagggtcct tcatcccagt gcaaacctaa ccatctcaaa
    27061 ctatctaaaa tcacttctac cgcttcttcg gtagagcgag ctacatcggt atcttcaatc
    27121 ctcaaaataa actttccgcc ttcttttcgg gcaaaagccc aattaaatag tgcagtcctc
    27181 gctcctccca gatgtaaaaa tccggtaggg ctgggcgcaa atcgagtcct cacttcagcc
    27241 aattttctca ctcctttccc ggggcacaat aaaagctcct ttcccccgag ccaccacttc
    27301 tccctcctga attacttctc cttgcacctc tattattctt ccattttttt taactactcg
    27361 accacgagcg acaagaggct tttcagttaa aagaggcttt atgtacctgg tttctaaagt
    27421 accggttact gcctcaattc ctgcattttt taagctatga gacatagctt catctaaaag
    27481 agccgctact atccctccat gcactacccc tttaaatcct tgataatacc agggaactga
    27541 agtttcagta acagtaaagt cgtcttcttg ccgaaatacc aggtggaaac cataaggatt
    27601 ttggctccca cagaataaac aattatcatc gtctataaaa ttcaatttct ttcctccatt
    27661 tccgaaagaa aaacgatttc cactcccggt tcggaaaaat tgctcacaaa ctggtaaata
    27721 gcttcataag tcacctcatt gggatgacca atggcaatag cctgaccatt tttcttcgct
    27781 atttctaccg ttttctgcaa ctgagtaata acgtaagaaa gagaaagttc tccatctaaa
    27841 aatacatccc ggcaaaaaga ggggattcct ttttgagaag ctaagcgata ggccagagaa
    27901 tgagaggaag ttaaactgtc cagaaaatat actccccgcc ccttcaagct ctccataaaa
    27961 tggctcatca gtaaatagtc agaggtagcc cgagaacctt tgtggttgtt tatcccttta
    28021 gcctggggaa ttagtcgcaa aacctcttct actttttctc gaacttcctc ctctctctct
    28081 cccacccgaa gcaaaaaggc ctctccacta ttctcccggt catcttctgc ctccatggga
    28141 aagtggataa ggatttcttt ccccttttct tcagctaaag aggcaatttt tttagccaaa
    28201 ggaaggtgtg gaaaaatggc aacattcagc ttcactggca ggtctaaaaa acgttcggcc
    28261 atttccagat tgtaacctaa atcatcaatt accagtgcta tctgagcccg gcgaggaata
    28321 aacgtctcca gaaagaacca gggttctgct ccccgaaaaa tagaatagaa gtcttttcct
    28381 ccttcttttt cctgctttac cacaaaacca aaagaggaaa gcagctggaa taaatccagc
    28441 atcttccggg aaaactcttc tcgagattcc tgatttgtaa gagtaacttg aacttcccag
    28501 cctccccctt cttccggagt cttaactacc tcgagtggtg catctttttt tatttcctcc
    28561 agaagaaaaa gagccagata tctctcttca ttttgaggat agagcctggc cagggttagg
    28621 taatgattac tcacctcttc cccttttttc cactctcccc acagaagaaa caaactccat
    28681 attaataaaa aaaggataac ccacagagcg ggtttacgaa accagggctt tctcatcgat
    28741 taatctacgt aactcttcct ccgctttttt aatttgctct tcttcctcac ttaccagaat
    28801 gtccggttct aatcccttac cattcaaatc cgttccatcg ggaaggagat acttagaaac
    28861 ggtaaaaatt actcctcctc catgagaaag aggaaaaatt tcctgaacta ctcccttgcc
    28921 aaaggttgtc tctcccacta attctgcacc taaatgaact cgtagaactc cagctaatat
    28981 ttctgcacca ctcgctgtcc ctttatttat cactaaaact atcggccctt tccacaccgg
    29041 atctacaaaa ttggtgatgc tttctctttt cccctctcta tcctctacaa tcaaaagagg
    29101 gttacctcga ggaacaaaca tacctgatgc taaaaccgca ctctgcagaa gcccacctgg
    29161 gttattgcgt aaatctaaaa ttaaaccttc cacccctgca acttgtaaac tactcaaggc
    29221 tttttttaac tcttcttgag ttcgaccatg aaactcagaa atacgaataa gaccaatact
    29281 cccggagagc atttcatatt ccacgctctg aattttgatt atatctctca ccacttccac
    29341 cacaatattt tcttcttctc cctccctctg gatagttaaa gtcaccgaag ttcctcgctc
    29401 tcccctcatc cttcgcaccg cttcatctga ggacataccc agagctggca ctccatctat
    29461 ttctacaatc acatctccag ctttaattcc tgctttctct gctggagagc cttgaaaagg
    29521 agaaataaca gtaagcttat catccttaat agctatgact atacccagac cggaaaattc
    29581 tccctctact ctatctgaag tttcaacctt ataatcttcc tcattcaagt agcgagcata
    29641 aggatcacca gttgctctta acacccctcg aatagcttct tccattagag ctcttccctc
    29701 aagaggcgtt tcagaataaa agctctcttg tagtagaaga agggtttcaa aaaagggctg
    29761 ccactcctcc aggtcgatag tccctcccaa accataagct cccttatttt cccagctctc
    29821 ccaggccaca attagtgcca ataaagcgat caaacagcca attagccaga tccttttatc
    29881 tctcattttt ctcctcctac ctcagccatt tgagggggtc ttcttcccga gcactacttc
    29941 ccaccctcac ctcaaagtga agaagaggcc tttctacaag accggtatca cccactgcac
    30001 caataacctc tcctcctttt actttttgtc ctccctctac ttttatctct gagagatgag
    30061 catacatagt tattatatct ccatgatcca aaagaatagt tttcccatag ccccggagag
    30121 tttgagccaa aatcactact ccatcgtgag cggcaaaaac cggactacct ttctctgctc
    30181 ctatatctat acccggatta taaaaggaaa ttccgtaacg ggggtctttt cgttctccaa
    30241 aaccttcaac aactgtgccc tgcaccggcc agaaaagacg cccct
//
LOCUS       DAOPSS010000005        25968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_1515_length_25968_cov_9.848802, whole genome shotgun sequence.
ACCESSION   DAOPSS010000005 DAOPSS010000000
VERSION     DAOPSS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 25968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25968
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1515_length_25968_cov_9.848802"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..405)
                     /locus_tag="PKN31_00815"
     CDS             complement(67..405)
                     /locus_tag="PKN31_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOA98500.1"
                     /translation="MEEEKKPDQYLFIREVASITQLHPQTLRNYEKWGLVKPRRSEGN
                     VRLYSLEDVEKIKKIKVFTQDLGINLAGVEVIFKLTEQIEELQKERDDIKKQLEEKNK
                     KIDTEPPVKE"
     gene            complement(415..1461)
                     /locus_tag="PKN31_00820"
     CDS             complement(415..1461)
                     /locus_tag="PKN31_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOA98501.1"
                     /translation="MEFKDYYKILGVSREADEKEIKKAYRRLARQYHPDLNPGNKEAE
                     QKFKEINEAYEVLSDPEKRKRYDELGSAWSSYGRQNTDEFWNDFYRRYGGGPTYTTFE
                     EEFEVGDFSDFFKTIFGDLLGGTRRASRERSSRFYTQDEGVRREAPSEVQSEVEVSFE
                     ESFKGTDRIIQVEYEEICPSCGGTGGNSEGKLCRRCGGRGVEKKSRKVNVTIPPGVSD
                     GTKLRIPQILGGRDLYLRVRVKPHPFFRREGDNVILELPITIYEATLGTEVEVPTLEG
                     KVKMKIPPETQNGTTFRLRGLGFPRTKGGGRGDQLVKVKVVVPRGLTDKEKKLLEELS
                     SLRKENPRQHLVRT"
     gene            complement(1517..1951)
                     /locus_tag="PKN31_00825"
     CDS             complement(1517..1951)
                     /locus_tag="PKN31_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOA98502.1"
                     /translation="MAINRWDPFRDLVELQEEINRVLGSSFPRRGKETTVGSWLPPVD
                     VKEEEEEFVLFVDLPGIDEKEVDITVVGDQLTIKGERKAPEEGEKFLRQERIFGPFNR
                     SFLLRVPVEVDKVSATYKNGVLEIHLPKSSAARPRKVNVQVE"
     gene            complement(2045..2599)
                     /locus_tag="PKN31_00830"
     CDS             complement(2045..2599)
                     /locus_tag="PKN31_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOA98503.1"
                     /translation="MEREEAWVLLKEHLKNHNLLKHSIACEVVMQFLARRLGEDEKEW
                     ALAGLLHDIDYEKTKDEPAEHGRWGAEILKEAGLPEEVIRTVQAHNPEATGIRPESPM
                     ERALYAVDPVSGFIVACALIHPEKKLESLDLSFLLNRFSEKSFARGANREAMKSIESL
                     GLSLEEFLEIALSAMQTKKEELGL"
     gene            complement(2601..3755)
                     /locus_tag="PKN31_00835"
     CDS             complement(2601..3755)
                     /locus_tag="PKN31_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA98504.1"
                     /translation="MGLKRKLLFLFLFLINTWIFPAWASSYHVVEQGDTLWAISRRYQ
                     IPIEKLLKLNNLTEKSTLRIGQKIKLEEGTEEAIYQIKAGDTLWGISRQFNIPLSSLL
                     AANGLSEKSVLRVGQKIILPSSSSSRKETTTTTVTSSNVTHTVEKGESLWLISRLYGV
                     DMDSIMKANNLTPDSILSIGMKLTIPQVRVASSASSSPPAKKESYEVYRIQSGDTLWG
                     ISRRYKVSLQDLMKVNNLNEKSTLQIGQSIRIPSHLPQNYEKASSSFLWPVSGRVSSS
                     FGMRGGRLHSGIDILAPSGTLIKASRSGVVSFSGWMNGYGRVVIVDHQDGWQTLYAHN
                     SVNLVNKGQKVNQGDAVARVGMTGNATTYHVHFEVRKNGKCLNPSDYLSK"
     gene            complement(3755..6913)
                     /gene="ileS"
                     /locus_tag="PKN31_00840"
     CDS             complement(3755..6913)
                     /gene="ileS"
                     /locus_tag="PKN31_00840"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOA98505.1"
                     /translation="MFKALGKLSCLAQKEEEILEFWKNRDIFKKSLKQREGSPRFTFY
                     EGPPTTNGYPHAGHVIGRSIKDLIPRYRTMCGYYAPRRAGWDTHGLPVELEVEKELGI
                     SGKPDIERYGVDKFNEKCKESVFKYIKEWEKLTERLGIWMDLEHPYITCSNEYIETLW
                     WVLKELWERELLYEGHKVLPYCARCGTSLSSHEVALGYQETEDPSLYVKFALRDREKT
                     YFLVWTTTPWTLAANVALAVGKNINYLEVVNEENGEHYILSEDSFYRLLGEDTHYKVI
                     RKYRGEELVGIKYEPLLDFVVAEKGYEVFSADFVTTEEGTGVVHIAPAFGEDDLQLAQ
                     QEDLPLLQPVRRDGKYGEEITPWQGMWVKDADPLIIDYLEEKGKLFKKETYRHSYPFC
                     WRCDTPLLYYAKESWFIRTTSFRDALLENNQKINWYPSHIKEGRFGDFLSNVVDWALS
                     RERYWGTPLNIWQCKNCNFKEAIGSIEELKNRSREKIEEIELHRPYVDRITLSCPQCG
                     GVMERVPEVVDCWFDSGAMFVAQLHFPFENEEEFQQSFPADFICEAIDQTRGWFYSLH
                     VLATALFNSPAYRNCLVTELGLDEKGQKMSKHVGNVVDPWDLIQDYGADALRWYVFSV
                     SPPWVPKRFGKEAIRGVLSKFFDTLWNVTNFFILYANIDGFEPPTSSFLPLEKRSVFD
                     RWIISRLQELIKEVRDSLDHYEVSRASKAIEDFVIEDLSNWYIRRSRRRFWKAEKDED
                     KVSAYHTLYQVLTSLTRLLAPFIPATAELLYQTLEADARGKRESVHLEDYPLPEEESI
                     DRELLLAMEVARKLTNLGRSVRNKVNIKLRQPLRKAIMVVPQEKERERALPFTSIIQE
                     ELNVKEIEWISHLPEEVTVSLKPRFSILGPRYGQKVKEIARLLKNLEEDKSRQFWEMG
                     EVSLLLEGEEVILKREEVELELQAREGLAVESEGEYAVILDTFLGQELQEEGMVRDLI
                     HAIQIWRKEAGLEVEDRIELEVREGTDPEWIDLIEKYSSLIQDEVLASSLSLCGDGER
                     EIISKEWELNGKKLVLALRRRL"
     gene            complement(6962..8266)
                     /locus_tag="PKN31_00845"
     CDS             complement(6962..8266)
                     /locus_tag="PKN31_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOA98506.1"
                     /translation="MIEVASLLKKEGIPGDLFTHRIFSQEVNYEANSFQGYKKEEREG
                     YGLRAINPEGKLGFYAFNRLLNPQEAVEKALEVSQLGDRVDFSIPTYPSTWEWEDFVD
                     PRVENLDAQEMIELGDYILSRIRKECPQALVDVVISAGREEKSLLNHHEEELKFARTF
                     FGVGVSANQTREGDILEVYAERSWGSRDIDVEGLVQEIVSKLEFSQRVADLNSGKYPV
                     VFTPSGSLVLLIALFYGLNGKNWVFGRSPLEDKEGKVIFSSLFSLEEIQEPWALGSCP
                     FDDEGIMTRAQKYLINKGKVESAFWDLWSAAKKGIKPTGNGFRGSYRDLPEPGFSSLR
                     VKNGLRDSSTLIGELEEGLIVDSVLGLGQSNVASGYFSCNVHLGFYVKGGEIQGRVKD
                     VMISGNTYQALFNLKEISRDSRWIGGRILLPYLLVEPIEVNK"
     gene            complement(8259..9635)
                     /locus_tag="PKN31_00850"
     CDS             complement(8259..9635)
                     /locus_tag="PKN31_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOA98507.1"
                     /translation="MMEVLEELIRLPRKGEYMEIRLENIFSTTIRVQNGVVERIRVGT
                     DEGGYIRLLAPRGSWWFESFQRWDDLKDKVEKAIAGASLLPQSNIEVVRRAPVRDEHK
                     VTLPDDFRFKSLEDKTSLLLLYDQVLRETSPFLVSSLVEYRDEFRQIYLANSDGSLIY
                     LEEPDISVSFVATARKEDNIEIYRNGVAGKAGFELVKNQETLAQEIGQRAVLLLEAPS
                     FSGGVYDVILDPVLAGVFIHEAFGHLSESDTLYRNEELKRIMAKGKRVSSSVLNVFDD
                     GSLDNLRGSSPYDDEGIPTGRTYLIKDGHLDGRLHSRETAFLMHEEVTGNARTINYRF
                     PPIVRMTNTAIEAGEASREDLFSDIERGLYAVEYIGGNTALELFTFSAAYGYLIEKGK
                     IKELVKDVVLSGNLFQTLDKIDAVADDFRWTEVGGCGKGGQIGLPTPTGSPHIRLREV
                     LVGGSHND"
     gene            complement(9632..10582)
                     /locus_tag="PKN31_00855"
     CDS             complement(9632..10582)
                     /locus_tag="PKN31_00855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOA98508.1"
                     /translation="MKKRAILWSVVLVLILSLGWGALAQETQEVQETQEVQETQEVQE
                     ESSEKTVVAEVNGEPIYLEDLQEIWDALPEVYRVQFPGGIKDLLEQLIRETLLAQQAK
                     KLGLEDDPVIQKRLEDIKRQLLVQELIKREIIDKVQVTDEEIEKEYTSNPSLYTEPEQ
                     VKAKHIMTSTEEKAQEVLQELQSGRPFEEVAMEKSESPDARQGGEMGYLKRGDLDPDL
                     EKVLFDLAPGKFSDIVETSYGFHIFLVEEHIDPRLKEVDEVKGEISNRLLPQKQQEAF
                     EKLIEELKSQAEIAIIEENLPKETTPQSEIPGETGGESQP"
     gene            complement(10582..10839)
                     /locus_tag="PKN31_00860"
     CDS             complement(10582..10839)
                     /locus_tag="PKN31_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98509.1"
                     /translation="MLLGILLIVVVLYGLLLFWVLNERLKDLDYLVSKVQESLKEVDD
                     KLVLLLSFKSEEEEKKKNDVLEETFSLHALQGEAKKKGDKS"
     gene            complement(10861..12114)
                     /locus_tag="PKN31_00865"
     CDS             complement(10861..12114)
                     /locus_tag="PKN31_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase family
                     protein"
                     /protein_id="HOA98510.1"
                     /translation="MSIKYAMILAGGKGDRLSVLSVERAKAAVPFGGCYRLIDFPLSN
                     CVNSGIYDVGVLTQYQPRSLIEHIGVGRPWDLDRKKGGIEFLQPYLSRTAKGWYRGTA
                     DAIYQNLNILSRKKIAHLLVLSGDHAYLMDYNPLYSYHLERDADVTLVATRVDKKDAP
                     RFGIMEVDGDNWITGFEEKPFSPRTDIAFMGIYVFRMEFLVEKLISNAREGKFDLVRN
                     IIVENVGKAKMQAYFYNDPWFDAGTVRAYWEANMHLLEPLPAFNLYNPDWMVFTNRGH
                     YPPTHTLEKADISSSIIGEGAVIKGKVVNSVIFPGVILEEGTEVVDSIIFNNTVVKEG
                     AKINLSILDKNIVVGQEAIIGYGDDFTPNEARPELLDWGVNLVGKGSEIPPRVIIHRN
                     CLIGIGIKEERFGERKELKSGEVLI"
     gene            complement(12124..13137)
                     /gene="galT"
                     /locus_tag="PKN31_00870"
     CDS             complement(12124..13137)
                     /gene="galT"
                     /locus_tag="PKN31_00870"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOA98511.1"
                     /translation="MPEFRKDPVVGRWVIVAEERGTKPYQFGEGWERESSFPCVFCPG
                     RESCTPPEVFSIREKDTLPNERGWRVRVVPNRFPALLPCEPLHQYIDGFYERMSGFGV
                     HEVIIETPDHYLDLVDLPLEQIEEVLRVYRERMRELGEDPRLRYCLIFKNQGEKAGAS
                     LPHAHSQLIATPVIPKKVQEELKRAREFYEEEGRCLFCSLLEEERKKERLVWEEEGFM
                     AFVPYAARFPYETWILPQRHCAFFSEIEEEEIQSLARILKIALSAIKALLHDPAFNFI
                     LHVAPYPRSSENYADYYHWHLEILPSLCKMAGFEWGTGFYINPVVPEKAAKALRQFIK
                     DKS"
     gene            complement(13159..14217)
                     /locus_tag="PKN31_00875"
     CDS             complement(13159..14217)
                     /locus_tag="PKN31_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOA98512.1"
                     /translation="MEKDQIDFLKNLINTPSPSGFEEKVQQVFLERIRDRVDQCYKDV
                     HGNAFGVVNPEGKVKVMLAGHCDEIGMMVEYINDQGYIYFSTVGGIDAHILPGKRVLV
                     HTSEGAIPGVIGKKPIHLLEEKDRQKVAKIEEQWVDIGAQSKEEALKQVTVGDPITFD
                     AGFEPLLGGRVAGKGFDDKVGTFIVGEVLRCLEKDKLHCAVYGVSTVQEELGLRGAKT
                     SAFGIAPDIGIAIDVTFASDCPDIDKRKVGEIALGKGPVISRGPNINPRLWQRMVNIA
                     KEGGIPYQVQAESRATGTDANVIQITREGVVTGLIGIPNRYMHTPGEIVDLEDLEHAI
                     KLLVRFLQSLEGNEEWIP"
     gene            complement(14236..16635)
                     /gene="lon"
                     /locus_tag="PKN31_00880"
     CDS             complement(14236..16635)
                     /gene="lon"
                     /locus_tag="PKN31_00880"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOA98513.1"
                     /translation="MEEEESANFLGNSPSSEENKENEFPEEISILPLKDNVLFPEIAL
                     PWVVTGEPWVRLINDAVLDKKILGVVSLRGEAKGEKLSPEDFYDVGVIGKISRLIKLP
                     DNAVQVLILGLVKFRIKEWIRWEPYPVAKIEVIKEEEVHSDEIEALIRNVVSLFQQVV
                     ELAPYLPREAFVTALNIKRPSRLANFIASNLNLDVESKMELLAAPDLSTKLTRLSFYL
                     TRELEILQIGSRIQSQVQQEMAKTQREYFLREQLKAIQQELGELDEKSLEIKELREKL
                     ESLDLPPEVRKEAEKEIERLSRLSPNSFEYPIIRNYLDWIIELPWNVSTEDNLDIDLA
                     KKILDEDHYDLEKVKERILEYLAVYKLKRDIKGPILCFVGPPGVGKTSLGKSIARALG
                     RNFVRISLGGIRDEAEIRGHRRTYVGALPGRIIQGIRKAGTNNPVFMLDEVDKIGMDF
                     RGDPASALLEVLDPEQNYAFVDHYLGVPFDLSRVMFIATANVLDTIPPPLLDRMEVIF
                     LSGYTDLEKIAIAQRYLIPKQCEENGLQPQDVIIEADIIQKIIREYTREAGVRNLERS
                     IATICRKIAKEVAQGAQGPFVVDEEKLREFLGPRRFLDEVMEGKDEIGVATGLAWTES
                     GGEILFVETVVLPGKGNLLLTGNMGEIMQESAKIALSCVRARAQEWNIPPDFYEKNDI
                     HVHVPAGAIPKDGPSAGITMVVSMISALARVPVRHDVAMTGEITLTGKVLPVGGIKEK
                     VLAAYRAGIREVILPRENEKDLQEVPEEVKKTMNFLLVENVNQVLDLALVKEELVEIQ
                     N"
     gene            complement(16680..17117)
                     /locus_tag="PKN31_00885"
     CDS             complement(16680..17117)
                     /locus_tag="PKN31_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOA98514.1"
                     /translation="MKKDFNNWWEEINYFQKEMQKFMKHISEGKVGFTEFGEETWVPY
                     CDIFETPQHVVIVIELAGVSPQEVEVIIEGKKLTVKGQRKELPYSSKEAYHLMEIRFG
                     PFYREVNLPEEVEEVRAKYSDGFLIIEGLKNIRERKISITQEI"
     gene            complement(17127..18650)
                     /locus_tag="PKN31_00890"
     CDS             complement(17127..18650)
                     /locus_tag="PKN31_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98515.1"
                     /translation="MKKIRGVLLLIFFLCSGWAGAQSFEFDVSSFEISQIDSLPLSPF
                     IFLLPDRGEWGLYYPGENIVLQYGSSKDGYVSIFDYTPDGKARIIKNNEPIAEGVQRK
                     IYGVVSGPEGVERFLMVLSDKIIPDRLLVEAMRNPSRIEEIVGYPVLLHYCRIQVVTE
                     RRTLAPSYISFDEVPGEIPAGGKLKVRAFLSDREGNALFNRPIYWEVNQGTLDNYETQ
                     TSTSGAVEVWYSAPSSLESTEVILKAYFPGDTIYEEAEGEIRFLVIPEKIQTTIVLTP
                     KSFHIAGGEVLDFQAELRDLRGKPVEGRSVRWEASIGSWEQEITYTDEAGITTNRLYA
                     PKVAGQETVEVKVSFAGVKNLLPSEDYAYGTISGLEVYPKEGLYFLDFTSGEPYTNFE
                     HLTYWGELLSGYAVNPAWVLSLLDGEFIEVGFSLSQPLHLGALYLWGKAQDEVFLKII
                     LNDQLIFSGRAAEGMVSPSEAQMIYIADYLKLGNNLLRIEVESSQGLYHLQRLLVVF"
     gene            complement(18653..19930)
                     /locus_tag="PKN31_00895"
     CDS             complement(18653..19930)
                     /locus_tag="PKN31_00895"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOA98516.1"
                     /translation="MYKREVLALILAGGEGKRLDILSEKRAKPAVPFGGKYRIIDFPL
                     SNCVNSGLYCVGIPTQYNPRSLHEHIGIGKFWDLDRMEGGVFLLQPYISDIVTHWYRG
                     TADAVYQNLRFIRDQDPYLVLILSGDHVYKMDYRQLIRFHQERKADLTIAVMEVPWEE
                     AHRFGVLSLDERGRVVDFEEKPPHPRSNLINMGVYVFDRHVLEEEVEEEALREGTSFD
                     FGKDIIPRMIGQRKVYGFCFSGYWKDVGTIDAYWEANMELLGSSPALDLYDKRWPIYT
                     YIEDRPPVKLSHRARIKRSLLGSGAIIDGSVEDSVIFSGVYVSRGAEVRHSIVMSDTL
                     IDEEAVVDRAILDKRIVVGKRAKIGYGEDLRPNKKIPDMLKSGLTIVGRNARIPPEAI
                     VGRNCRIGVDIKEEDFTSLILESGESIEKAEEG"
     gene            complement(19909..21477)
                     /locus_tag="PKN31_00900"
     CDS             complement(19909..21477)
                     /locus_tag="PKN31_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOA98517.1"
                     /translation="MAKKLMVNITWHMHQPFYWNEKKGVFDFPWVRFHATKDYLFMAK
                     LVEKFPSLRVTFNFAPCLLRQFQLYLEGEEDLVMKLAKKKASAISEEDKKLIKEKFFI
                     VSSPRTYEKWPRFAELKEKPWESFTSQDLLDLQVLYQLIWFDPLWREENPVGRELWER
                     GRNYREEDKEKIWKATEEIFKDIFACYRGLLEKRQIEISFSPLYHPILPLLINTDVAR
                     EANPSTILPHRFSFPEDAREQINKGKDFSQDIWGSDIMGMWPSEGAVSEEAIYLAIQE
                     GVKWLATGEEILFRSLGKKKETAWQELYLPHVWAKDGKEIAVFFRDTGLSDLIGFSYQ
                     HFPPQEAVKDFMNHLRAIREALPEDKPYLVNIILDGENAWEYYVDNGLPFITSLYQTL
                     SESEEFVTTTPLEYLGNFSHLPQISRIVPGSWICGNLNTWIGHPEKNWAWEELRRVRE
                     LWDREKEYLSKGERERIQDLIYQAEGSDWFWWLGDDNPSSQKESFRQHFLYLIHSVLR
                     ALAKGEKECIKEKF"
     gene            complement(21488..21982)
                     /locus_tag="PKN31_00905"
     CDS             complement(21488..21982)
                     /locus_tag="PKN31_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOA98518.1"
                     /translation="MKIAVISDTHIPQRGRNLPPYLLRFLEGVSLILHAGDITEWWFL
                     EELMKYAPVRAVRGNMDSPLVRERLSDKEIVEVEGVKIGIAHGEGSPAGIEERVGKLF
                     NGENLQVIVYGHTHQPSKEWRGEVLYFNPGSPTDWVFAPYFSFGFMELREGKIIEAEI
                     LKVD"
     gene            complement(22058..22930)
                     /gene="folD"
                     /locus_tag="PKN31_00910"
     CDS             complement(22058..22930)
                     /gene="folD"
                     /locus_tag="PKN31_00910"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOA98519.1"
                     /translation="MSAQILDGKKTSAEVKEELKPRIAYLTERGCQPGVAVVLVGDDP
                     ASQIYVRNKERTCEKLGIYSVQHRLPATTPQEELIELVNNLNQNERIHGILVQLPLPP
                     HIQEREVLYHISPEKDVDGFHPYNLGRLMIGDPVFLPCTPWGVQELLLRYNLSPEGKN
                     VVIVGRSNIVGKPLAMMLVQKGRGANATVSMCHTGTRNLSTYTRMADILIAACGSPRL
                     ITPDMVKEGAVVVDVGINRVGDKTVGDVDFEGVKEVASYISPVPGGVGPMTIAMLMAN
                     TVKAAERALQRKNA"
     gene            complement(22934..23647)
                     /locus_tag="PKN31_00915"
     CDS             complement(22934..23647)
                     /locus_tag="PKN31_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOA98520.1"
                     /translation="MNLVGDLHTHTVASGHGYSTVMEIAQVAKRKKLEVVAITDHSPE
                     MPGGPLPIYFESHGHFPREIAGVKIYFGVEADILDEKGNLALDEKLLKKLDIVIVSFH
                     PQIFPPASREINTQALLRAIENPLVDIIAHPGNPRYPLDYERVVEAAVSRGKVIEINN
                     SSFTITRRGSRENCRLIAQEVKKQGGWVAVSSDAHFCETVGEFKEAQGLLEEVGFPPE
                     RVLNSSRDNIEKFLNRERR"
     gene            complement(23658..24830)
                     /locus_tag="PKN31_00920"
     CDS             complement(23658..24830)
                     /locus_tag="PKN31_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98521.1"
                     /translation="MAKRKKSLVFSQGEKGSFFSQEERAELLDKLPLLFEEEEVIVEQ
                     FFSFYRAVLREKKNLIFLSSPVVVSFLRNFLMEEVSSRGEQFIGLVDSSRPDLLLYWK
                     EKLPPKDTALVVLARPLEELQLLLLAFSFPQWEKVLVGSNYGILAQSAQILFLPFLPT
                     EIYHHYFWQRSALLYLPLISLNLPVEELEEGFKKSDYLREEAAALSLFIKEIEERGIE
                     RVNFLVDNSFILSILQSFIPLLEYSCKGEGKMEFRAFDFSMMRDGQLFLSDNSIFILV
                     KSEKINKIKFTLPSALKRIDLFSPEGSVPTSGSWKEIKEIECRVVENLLYKEGKDFLK
                     WEYYLPDFQTTGELMTFVRYLVSYNAWLRGLDLWSDPPVAEFDNQIWRLLASNKKQ"
     gene            complement(24806..>25968)
                     /gene="cimA"
                     /locus_tag="PKN31_00925"
     CDS             complement(24806..>25968)
                     /gene="cimA"
                     /locus_tag="PKN31_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOA98522.1"
                     /translation="YDAEHFFDGFKDNPEYALKTLQVAHEAGAQILVLCDTNGGTLPL
                     EMEVIINRMKEFFGGDEGIKWGIHAHNDSGVAVANSLIAVHLGAQQVQGTINGYGERC
                     GNANLCTIIPNLKLKMNIHPITDEEMRKLRETSYFVSEIANLRPNDYDPYVGRSAFTH
                     KGGIHVSAISRYPATYEHASPELVGNERRILVSEQAGRSNLTYRARELGIELDPKDPR
                     LLTLIQKIKEAENYGYQFEAADASFELMLREALGESAEFFDLEGFRVIVEKRGEEDTV
                     AEATIKVKVNERMIHTAAEGDGPVHALDNALRKALEEMYPLLKEIRLSDYKVRVLNEK
                     EGTAARIRVLIQSTDGRDSWGTVGVSADIIEASWQALTDSIKYGLWRREKKA"
BASE COUNT         7275 a         6527 c         4359 g         7807 t
ORIGIN      
        1 gagctaaaga gataacttct ttagcttttt cagtaaattg ttcaaacctc attttatctt
       61 cctccttcac tctttaactg gaggttcagt atcaattttt ttattttttt cttctaattg
      121 tttctttatg tcgtctcttt ctttctgcaa ctcttctatc tgctcagtga gcttaaaaat
      181 cacttctact cctgccaggt ttattcctaa atcttgggta aaaaccttta tttttttgat
      241 tttctccacg tcttccaaac tataaagccg aacattaccc tcgctacgac ggggtttgac
      301 taacccccat ttttcataat tacgcaaggt ttgaggatgc aattgagtta tacttgccac
      361 ctctctaata aacaggtatt gatcgggttt tttctcttct tccatatcat caccttaagt
      421 tctcaccaaa tgctgacgag gattctcctt gcgcagggaa gataattcct ccaataattt
      481 cttttctttg tcggttaaac ctcgaggcac taccacttta actttcacca gctggtctcc
      541 tcttccccct cctttagtcc ggggaaagcc taatcctcgc aggcggaagg tagtcccatt
      601 ttgagtttca ggaggaattt tcattttgac cttaccttct aaagtgggga cttccacctc
      661 tgtacctaaa gtcgcttcgt aaatagtaat tggcagttct aaaatgacat tatccccttc
      721 ccgacggaaa aagggatggg gttttacccg gactctcaag tacaggtctc ttcctcccag
      781 tatctgggga attctcaact tcgtaccatc acttactcca gggggaatgg taacatttac
      841 cttccgagat ttcttttcta ccccccttcc tccacacctc cgacatagtt tcccctcact
      901 atttccaccc gttcctccac aactggggca tatttcttca tattctactt gtataattcg
      961 gtctgttcct ttaaaacttt cctcaaaaga aacctctacc tcactctgta cttcagaagg
     1021 tgcttccctt cttactcctt cgtcttgagt ataaaaacgg gagctccgtt cccgggaggc
     1081 tcgacgagtt cctcctaata aatcaccgaa tatagtctta aagaaatcgc tgaaatctcc
     1141 aacttcaaat tcttcctcaa aagtggtata agtgggccct cccccatatc ttctataaaa
     1201 atcattccag aactcgtcag tattctgtct tccataacta ctccaggccg atcctaactc
     1261 atcatatctt ttcctttttt ccggatcgct caatacttca taggcttcat tgatctcttt
     1321 aaatttttgt tccgcctctt tgttgccagg attgagatct ggatgatact gacgagccag
     1381 acgtcgatag gcttttttta tctctttttc atcagcttct cgagaaactc ccaaaatttt
     1441 atagtaatct ttgaattcca attttcatca cctctggggg tgggagtttt ccacccccag
     1501 aactatttca cctcttttac tctacctgaa cgtttacttt ccttggtcta gcagcagaag
     1561 atttaggtaa atggatctct aaaactccat ttttataagt ggctgatact ttgtccactt
     1621 ctacaggtac ccggaggaga aaagaacggt tgaatgggcc aaaaattcgc tcctgtcgca
     1681 aaaatttctc cccttcttcc ggtgcctttc tctccccttt aatggttagc tggtctccta
     1741 ccacggtaat atccacttct ttttcatcta tcccgggtaa gtcaacgaac agcacaaact
     1801 cctcctcttc ttctttcaca tctacaggag gaagccagct tcccacggta gtttctttcc
     1861 ccctacgagg aaaagaactc cctaaaacac ggttaatttc ctcctgcagt tcaactaaat
     1921 cccgaaaagg atcccaacga ttaatagcca ttttatctta cctcctttga cctttcttga
     1981 ccttgcaaag caattataaa agaagcccat aattttgtca agaatagtca aacaccaccg
     2041 aaaatcataa gcccagttcc tcttttttag tctgcatggc agaaagagcg atttccaaaa
     2101 actcctccag agagagacct aaactttcta tgcttttcat tgcttctctg ttagcccctc
     2161 gagcaaaact tttttccgaa aaacgattaa gaagaaaaga aaggtctaaa ctctccagct
     2221 tcttttccgg atgtatgagg gcacaggcta cgataaaacc acttaccgga tctacagcat
     2281 aaagagctct ctccatagga gactcaggcc taataccagt ggcttcgggg ttatgagcct
     2341 gaactgttct gattacttct tcaggaagac ctgcttcttt gagtatttcg gctccccacc
     2401 tcccgtgctc tgcgggttca tctttagttt tttcataatc tatatcatgc aaaagtccgg
     2461 ccaaagccca ctctttttcg tcttctccca gacgacgggc taaaaactgc ataacgacct
     2521 cacaagctat actgtgtttc aaaagattat ggtttttcaa gtgttctttc agtaaaaccc
     2581 aggcttcttc tctttccatt ttacttgctc aaataatccg aaggattaag acatttacca
     2641 tttttcctca cttcaaagtg aacatgatag gtagtagcgt ttcccgtcat ccccactcga
     2701 gcaacagcgt ctccctggtt tactttttgt cctttattaa ccagattaac tgaattatga
     2761 gcatacaaag tttgccagcc atcttgatgg tcaactatta ctactctacc atatccattc
     2821 atccacccgc taaaagaaac tacaccactg cgagaagctt taatcagcgt acctgaagga
     2881 gctaaaatgt caatcccgga gtgaagcctt cctccccgca taccaaaaga agaagaaact
     2941 cgaccagaaa caggccagag gaaagaacta ctggcctttt cataattttg aggaaggtga
     3001 gaaggtatgc gaatactttg ccctatttgc aaagtgcttt tctcattaag attgttgact
     3061 ttcattaaat cctgcaggga aactttataa cgacgggata ttccccataa cgtgtctccc
     3121 gactgtatcc gataaacttc atagctttct tttttcgctg gaggagaaga agaagcagaa
     3181 gaagcaacac gaacctgggg aatagtaagt ttcataccaa tggagagaat gctatcagga
     3241 gtgagattat tggctttcat aatggagtcc atatccaccc cataaaggcg ggatatcaac
     3301 cacaaggatt ctcccttttc caccgtgtgg gtaacattag aggaggtaac agtagtggtg
     3361 gtggtttctt ttctcgaaga agatgaagag ggcaaaataa ttttctgtcc tactcgaagg
     3421 acagatttct ccgagagacc attagctgca agaagagaag aaaggggaat gttaaactga
     3481 cgtgatatcc cccagagagt gtctcccgcc ttaatctggt aaattgcctc ttcagttcct
     3541 tcttctaatt taattttctg tcctatccga agggtagatt tttccgtaag gttattaagc
     3601 ttcaaaagtt tttcgatagg tatctggtaa cggcgagata tggcccagag agtgtctcct
     3661 tgttctacta cgtggtaact acttgcccag gcaggaaaaa tccaggtgtt gattaaaaac
     3721 agaaaaagga aaagaagctt tctttttaac cccattacaa tctcctcctt aaagccagta
     3781 ctaatttttt tccgtttagc tcccattctt tgctaattat ttctctttct ccatcaccac
     3841 ataaagaaag agaagaagct aaaacctcgt cttgaatcaa ggaactatat ttttctatta
     3901 aatctatcca ttccgggtca gttccttctc tgacttctaa ttcaattcgg tcttctactt
     3961 ccaagccggc ttctttgcgc cagatttgga tggcatgtat taaatccctt accatccctt
     4021 cctcctggag ctcctggccc aagaaagtat ctaaaataac cgcgtattct ccttcacttt
     4081 ctaccgcaag accttctcga gcttgcaact ctaactctac ttcttctcgc tttagtataa
     4141 cctcttctcc ttctaaaagt aaagacacct ctcccatttc ccagaattgt cgacttttgt
     4201 cttcttccaa attttttaat aaccgggcta tttcctttac tttttgacca tatcgaggac
     4261 ctaaaatgga aaaacgaggt ttcagagaaa cagtaacctc ttcaggaaga tgagaaatcc
     4321 attctatctc tttaacattt aattcttctt gaataataga ggtaaaagga agggcccgtt
     4381 ctctttcttt ctcctgggga actaccataa tcgcttttct gagaggctgt ctcagtttga
     4441 tattcacttt attacgaacc gaacgaccta aattggtaag cttccttgct acctccatag
     4501 ccaatagtaa ctccctatct atgctttctt cctctggtag aggatagtcc tccagatgaa
     4561 cgctttctct ttttccccga gcatcagctt ccagagtttg gtagagtaac tcagcagtag
     4621 caggaataaa aggagctaaa agtcgggtta aactggtaag aacttgataa agagtgtgat
     4681 aagcgctgac tttatcttcg tctttttccg ctttccagaa acggcgacga gagcgcctga
     4741 tgtaccaatt gcttaaatct tctattacaa aatcttctat agctttactc gctcgagaga
     4801 cttcatagtg gtctaatgaa tctcgaactt cctttatcag ttcttgcaag cgagatataa
     4861 tccaccgatc aaaaacactt cttttttcca gaggcaaaaa agaagaagtt ggtggctcaa
     4921 aaccatcaat attagcgtaa agaataaaga agttagttac attccataag gtatcaaaaa
     4981 atttactgag aactcctctt atcgcctctt ttccgaatct cttgggcacc cagggaggag
     5041 aaacagaaaa aacataccag cgcaacgcat cagctccata gtcctgaatc aaatcccagg
     5101 ggtcaaccac atttcccacg tgtttgctca ttttttgccc tttctcatcc agccccagct
     5161 cggtaaccaa acaattgcga tacgccggac tattaaaaag agcagtagct aaaacatgaa
     5221 gactataaaa ccatccccgg gtctggtcta tggcctcaca gataaagtca gcaggaaaac
     5281 tttgttgaaa ttcttcttca ttctcaaaag gaaagtgaag ttgcgctaca aacattgctc
     5341 cagaatcaaa ccagcagtca actacttctg gcactctttc cataactcct ccacattgag
     5401 ggcaagaaag agtaattcta tccacgtagg ggcgatgcaa ttcgatttct tctatttttt
     5461 ctcgtgatct gttctttaac tcttctatgg aaccaatagc ttctttaaaa ttgcaatttt
     5521 tgcattgcca gatattgaga ggtgttcccc agtatctctc tcgagataga gcccaatcca
     5581 ctacgttgga taagaaatct ccaaatctcc cctctttaat gtgagaagga taccagttaa
     5641 ttttttggtt attttctaac aatgcatctc gaaaagaagt agttctaata aaccagcttt
     5701 ccttagcgta gtagagtaaa ggagtatcac acctccagca aaaaggataa gaatgccggt
     5761 aagtttcctt tttaaataac ttcccctttt cttctaaata atcaataatc aaagggtcag
     5821 cgtcctttac ccacattcct tgccagggag taatttcctc tccatatttt ccatctcgtc
     5881 ttactggttg taagagggga agatcttctt gctgagctaa ctgcaggtca tcttcaccaa
     5941 aagcaggagc aatatggact actccagtac cttcttccgt ggtgacaaaa tcggcagaaa
     6001 aaacttcata tcctttctct gccactacga aatccagcag gggttcgtat tttattccta
     6061 ctaattcctc tccccgatat tttctgatta ctttataatg ggtatcttct cccagtaggc
     6121 gataaaagct atcttcgcta aggatataat gttcaccgtt ttcctcattg accacttcca
     6181 ggtaattaat attcttacct actgcgagag ccacgttggc tgccagagtc cagggggtgg
     6241 tagtccacac cagaaaataa gttttttctc tatcccgaag agcaaactta acataaagcg
     6301 aagggtcttc cgtttcctgg tagcccagag ctacttcatg gctggaaaga gaagtcccac
     6361 aacgagcaca gtaaggcaag accttatgcc cttcatatag taattctctc tcccagagtt
     6421 cttttaacac ccaccacaaa gtctcaatgt attcattact acaggtaatg taggggtgct
     6481 ccaaatccat ccagataccc aatctctcgg ttagcttttc ccattcttta atatacttaa
     6541 aaacactttc tttgcacttt tcattaaatt tatccactcc atagcgctca atatccggtt
     6601 ttcccgaaat tcctaattct ttttctactt ccagctctac cggaagacca tgggtatccc
     6661 agcctgctcg gcgaggagca taataaccgc acatagtccg ataacgagga atcaaatcct
     6721 taatgctgcg accgattaca tggccggcat gaggataacc attagtagta ggaggacctt
     6781 cgtagaaagt aaaacggggt gagccctctc tttgttttag gctcttttta aatatatctc
     6841 tgtttttcca gaattctaaa atttcttctt ctttttgggc caaacaagaa agcttgccca
     6901 gagctttaaa cattaatcct caccttccta cttttctcga tagactttta ctgcttgata
     6961 actacttgtt gacttctatt ggctctacta aaaggtaggg aagcaaaatt ctacccccta
     7021 tccagcgact atcccgagaa atttctttaa ggttaaaaag agcctgataa gtatttccac
     7081 tgatcatcac atctttcacc cgcccctgaa tttctcctcc tttaacgtaa aaacccaggt
     7141 ggacattaca ggagaaataa cctgaggcaa cattgctctg tcccagacct aaaacactat
     7201 ccactatcaa accttcttcc agctctccta ttaaagtgct actatcccgc aaaccattct
     7261 taacccggag agaggaaaat cccggttcgg gtaaatcccg gtaggaaccg cgaaaaccgt
     7321 ttcccgtggg tttaattccc ttctttgctg cgctccataa atcccagaaa gcgctttcca
     7381 cctttccctt gttaattaga tatttctgag ccctggtcat gattccttca tcatcaaaag
     7441 gacaggagcc cagagcccag ggctcttgta tttcttctaa agaaaaaaga gaggaaaata
     7501 ttactttccc ttctttgtcc tccagaggcg accttccaaa tacccaattt ttaccattta
     7561 atccataaaa gagagcaata agtaaaacca gactaccaga aggagtgaaa actaccgggt
     7621 acttcccact gtttaaatca gccactcttt gagagaactc cagcttggaa actatctcct
     7681 gcactaaacc ttctacatca atatcccggc tgccccagct tctttccgca taaacttcta
     7741 aaatatcccc ttccctggtc tgattggcac ttacccctac tccaaaaaat gttcgggcaa
     7801 acttcaattc ttcttcatga tgattcaaaa gagacttttc ttccctccca gcactgataa
     7861 ctacatctac cagagcctgg ggacactctt tccttattcg ggaaagaata taatctccca
     7921 gctctatcat ctcttgagca tctaaatttt ccacccgggg gtcaacaaaa tcttcccatt
     7981 cccaggtaga tggataagta ggaatagaaa aatctaccct gtcccctaac tggctaactt
     8041 ctaaagcttt ctccacagcc tcttgaggat tcaaaagacg attaaaagcg taaaatccta
     8101 atttcccctc cggattaatt gctcgcaggc cataaccttc tctctcttcc tttttgtatc
     8161 cttgaaagga attagcttca tagtttactt cctggctgaa gattcggtga gtaaataaat
     8221 caccaggtat accctctttt ttaagcaagc tcgctacttc aatcattatg gctacctccc
     8281 accagaactt ctctcaaccg tatgtgcgga cttcctgtag gagtaggaag tcctatctgt
     8341 ccacctttcc cacatccccc cacttctgtc cagcgaaaat catcagcaac tgcatctatt
     8401 ttatccagag tttgaaataa atttccactc aataccacat ctttaactaa ctcctttatt
     8461 ttaccctttt caatcaagta accataagca gcagaaaaag taaatagctc gagagcagta
     8521 ttccctccaa tatactctac cgcatacagc cctcgttcta tatcagaaaa taaatcctcc
     8581 cgagaagctt cccctgcttc tatagccgta ttggtcatcc gtactatggg aggaaaacga
     8641 tagttaatgg tccgggcatt accggttacc tcttcgtgca tgagaaaagc ggtttcacgg
     8701 gaatggagtc tcccatccag gtgaccatct ttaataagat aggttctgcc tgtaggtata
     8761 ccctcatcat cataaggaga agatcctcgc aaattatcca ggcttccatc atcaaaaacg
     8821 ttcaacactg acgaggatac tcttttgcct ttagccatta ttcttttcag ctcttcattg
     8881 cgataaagag tgtcagattc actaagatgt ccaaatgcct cgtgaataaa aaccccggca
     8941 agaactggat ctaaaatcac atcatacact cctcccgaga aagaaggagc ttccaaaaga
     9001 agaacagctc tttgtcctat ctcctgagcc agggtttcct gatttttaac cagctcaaat
     9061 cctgctttgc ccgctactcc attcctgtat atctctatgt tatcttcctt tcttgccgta
     9121 gccacaaaac tcacgcttat gtcaggctct tccagataaa ttaaagaacc atcggagtta
     9181 gctaaataaa tctgccgaaa ttcatcccgg tattctacta aagaacttac caaaaaagga
     9241 gaagtttctc tcaaaacctg atcatacaag agaagaagag aggttttatc ttcaagggat
     9301 ttaaaacgga agtcatcggg aagagtaacc ttgtgttcat cccgaactgg cgctcttctt
     9361 accacttcaa tgttgctttg aggcaagaga gaagcgccag ctatagcttt ttctacctta
     9421 tctttcaagt catcccatcg ctgaaaactt tcaaaccacc agctacctcg aggggcaagg
     9481 aggcggatgt aacctccctc atccgtcccc actcttatcc tctccactac cccattctga
     9541 actcggatag tggtagaaaa aatattttcc aagcggattt ccatatactc ccccttccgg
     9601 ggaaggcgaa tcagctcctc cagaacttcc atcatggctg gctttctcca cctgtctctc
     9661 caggaatttc tgactgagga gttgtttcct taggaaggtt ttcttctatt attgcgattt
     9721 ctgcctggct ttttagctct tcaattaact tttcaaaagc ctcttgttgt ttttgaggaa
     9781 gaaggcggtt agatatttct ccttttacct catctacctc cttaagacga gggtcaatgt
     9841 gctcttctac cagaaaaata tgaaaaccat agctggtttc cacaatatca ctaaatttgc
     9901 ctggagcaag gtcgaaaaga actttttcta aatcagggtc caggtctcct cttttcagat
     9961 aacccatttc tcctccctgc cgagcatcgg gagattctga cttttccatc gctacttctt
    10021 caaacggcct gccagattgt aattcctgca aaacctcctg cgctttctcc tcagtagagg
    10081 tcataatgtg tttagcctta acttgctccg gttcagtata aagagaaggg ttagaagtat
    10141 actctttttc gatttcttca tcggttacct gcactttgtc tattatctct cttttaatca
    10201 actcctggac taaaagctgc cgcttgatat cctctaatcg tttttgaata accggatcat
    10261 cttctaaacc cagcttttta gcttgttgag ctaataaagt ttctcgaatt aactgctcta
    10321 aaagatcctt aatgccccct ggaaactgga ctcgataaac ttctggaagc gcatcccaga
    10381 tttcctgtaa atcctcaaga taaataggtt ccccgtttac ctcagccact acggtttttt
    10441 cgctactttc ttcctgaact tcctgagttt cctgaacttc ctgagtttcc tgaacttcct
    10501 gagtttcctg agctaatgcc ccccacccta aactcaaaat cagaactaaa accactgacc
    10561 aaagaattgc tcgcttcttc atcaagattt gtctcctttc tttttggctt ctccctgcaa
    10621 agcatgaagg gaaaaagttt cttctaaaac atcgttcttt ttcttttctt cctcctcact
    10681 tttaaagctc aataaaagaa ctaacttatc gtctacctct ttcaaacttt cttgtacttt
    10741 actaactaaa taatccagat ccttcaacct ttcattaagc acccagaaaa gcagaagtcc
    10801 atacagaacc accacgatta acaaaatacc cagaagcaat ttctctacct cccgtctacc
    10861 tcagatcagg acctcgccac ttttaagctc ttttctctca ccgaaacgct cttcttttat
    10921 ccctatacct ataagacaat tacggtgtat aataacacga ggagggattt cactcccctt
    10981 ccccactaaa ttaactcccc aatctaaaag ctcaggacga gcctcattgg gggtaaaatc
    11041 atctccatag ccaataatgg cttcttgtcc taccactata tttttatcta aaatacttaa
    11101 atttatttta gccccctctt ttactactgt attattgaaa ataattgaat ctactacttc
    11161 tgttccttct tccagtatta ccccggggaa aatcacagaa ttaaccactt tccccttaat
    11221 taccgcccct tctccaatta tactggacga tatatctgct ttttccagag tgtgggtagg
    11281 aggatagtga ccccgattgg taaaaaccat ccagtcagga ttataaagat taaaagcagg
    11341 taggggttct aaaagatgca tattagcttc ccagtatgct ctaactgttc ctgcatcaaa
    11401 ccaggggtca ttgtagaaat aggcttgcat tttagctttc cctacatttt cgactatgat
    11461 attacgaacc aggtcaaact ttccctcccg agcattgctt atcaattttt ctactaaaaa
    11521 ttccatccga aaaacgtata tacccataaa agcaatatca gtgcgaggag aaaaaggttt
    11581 ttcttcaaat ccggtaatcc agttgtctcc gtctacttcc atgataccaa aacggggagc
    11641 atccttttta tctacccggg tagccaccag ggtcacatca gcatctcttt ctaagtgata
    11701 agaatacaaa gggttatagt ccatcagata agcgtggtca ccagaaagaa ctaaaagatg
    11761 agctatcttc ttgcgactta agatattgag gttttgatag atggcatctg ctgttcctcg
    11821 ataccaaccc ttagctgttc tactcaagta gggttgcaaa aactctatcc ctcctttctt
    11881 ccggtccaga tcccaggggc gtcccacccc gatatgttca ataagagaac gaggctgata
    11941 ttgagtaagg acaccaacat catagatacc cgaatttaca cagttactga gaggaaagtc
    12001 gataagacgg taacatcctc cgaaaggaac agccgctttt gccctttcca ctgaaagaac
    12061 actcaagcga tctccttttc cacctgccag aatcatagca tatttaatag acaaattcct
    12121 cacctaactc ttatctttta taaattggcg aagagctttc gccgcttttt ctggaaccac
    12181 cggattaatg taaaatcccg taccccactc aaatcctgcc atcttgcaca aggaaggtaa
    12241 aatctccagg tgccagtggt agtagtctgc ataattttcc gaggagcggg gataaggcgc
    12301 cacatgtaga ataaaattga aagccgggtc atgaagaagg gctttaattg cagaaagagc
    12361 tatcttcaga atgcgagcaa ggctttgtat ttcctcttct tctatctctg agaaaaaagc
    12421 acaatgacgc tggggaagaa tccaggtttc ataaggaaaa cgagcagcat agggaacaaa
    12481 agccataaat ccttcctctt cccagaccaa tcgttccttt tttctttctt cttccagaag
    12541 agaacagaaa agacaccttc cttcttcctc gtaaaattct cttgctcttt ttaattcttc
    12601 ctgcactttt ttaggaataa cgggtgtagc aattaactga gagtgagcgt gaggtagaga
    12661 agctcctgct ttttcacctt gattcttaaa aatcaaacaa taacgcaggc gagggtcttc
    12721 tcccagctct ctcattctct ctcggtaaac ccggagtacc tcttctattt gttccaaggg
    12781 aaggtcaacc aagtccaaat agtggtcagg agtttctatt attacctcgt gcaccccaaa
    12841 gccactcatc ctctcgtaaa atccatcgat atattgatgc aagggctcac acggtaaaag
    12901 agcaggaaat ctattaggca ccaccctcac cctccagccc ctctcattgg ggagagtatc
    12961 tttttcccgg atgctgaata cttccggagg agtacaagac tctctacccg ggcaaaacac
    13021 acagggaaaa gaactttccc tttcccaacc ttcaccaaac tgataaggct tggttcctct
    13081 ttcctcggca acaatgaccc atctccctac tactggatct tttctaaact ccggcatcta
    13141 aaaattaccg ttctcctctc aaggaatcca ttcttcattt ccttccaaac tttgtaagaa
    13201 tcgcactaaa agcttaatag catgttccag atcttctaaa tccactattt ctcctggagt
    13261 gtgcatataa cggttaggga taccaatcaa ccctgtaact actccttctc gagtaatctg
    13321 aatgacatta gcgtcagtcc ctgtagcccg ggattcagct tgcacctggt aaggaatgcc
    13381 tccttctttg gctatattta ccatccgctg ccagagacga ggatttatat tgggacctcg
    13441 agaaatcacc ggacccttac ctaaagctat ttctcccact tttcttttgt ctatgtccgg
    13501 gcaatcagag gcaaaagtaa catcaatggc aatacctata tctggagcta taccgaaagc
    13561 actggttttg gctcctcgca gtcctaactc ctcttgcacc gtgctcactc catagacagc
    13621 acagtgtaat ttatcttttt cgagacagcg tagcacttct cctactataa atgttcccac
    13681 tttatcatcg aagcctttcc ctgctactct ccctcccaga aggggttcaa aacccgcatc
    13741 aaaagttatg ggatcgccta cagtaacttg cttcaaggct tcttctttgc tttgagcacc
    13801 aatgtctacc cattgttctt caattttggc caccttttgt cgatctttct cttccagaag
    13861 atggataggt tttttgccta tcacccccgg tatcgctcct tctgatgtat gaacgagcac
    13921 tcttttcccg ggaagaatat gagcatcgat tccccccacc gtagaaaaat aaatatatcc
    13981 ctgatcgttt atatattcta ccatcattcc tatctcatca cagtggccag ccagcataac
    14041 tttgactttt ccttctggat tgactacccc aaaagcatta ccatgaacat ccttgtaaca
    14101 ttggtctact cgatctctaa ttcgctccaa aaaaacttgt tgcactttct cctcaaaacc
    14161 ggaaggactg ggcgtattaa tcagattttt aagaaaatct atctggtctt tctccataat
    14221 gttttaccta cctcctcaat tttgaatttc tactaactct tctttaacta aagctaaatc
    14281 cagaacttgg tttacatttt ctaccaggag gaaattcatt gtttttttaa cttcctctgg
    14341 cacttcctgt aaatctttct cgttttcccg ggggaggatt acctctctaa tccctgcccg
    14401 ataagcagct aaaacttttt cttttattcc gccaacaggt aatactttgc cagtaagagt
    14461 aatttccccg gtcatagcca catcatgacg aacaggtact cgagccagag cagaaatcat
    14521 agatactacc atggtaattc ctgctgacgg cccatctttg ggaatagcac cagcaggaac
    14581 atgcacatga atatcgttct tctcataaaa atcaggagga atattccact cctgtgctcg
    14641 agcccggaca caggaaagag caattttagc tgactcctgc ataatttctc ccatgtttcc
    14701 agtcaaaaga agatttccct tacccggtaa aaccactgtc tctacaaaaa gtatttctcc
    14761 tccactttcc gtccaggcta aacccgtcgc cactcctatt tcatctttcc cttccatcac
    14821 ctcatccaaa aatcggcgag ggcctaaaaa ttctcttagc ttttcctcat ctaccacaaa
    14881 aggaccctga gccccctgag ctacctcttt agctattttt cggcaaatag tagcaatact
    14941 tctctccaaa ttccgcactc ctgcttctcg agtgtattct ctgattattt tctgaataat
    15001 atctgcttct atgatgacat cctggggttg aagaccattc tcctcacatt gtttaggtat
    15061 aagatatcgc tgagcaatgg ctattttttc taaatcggta tagccagaaa gaaaaataac
    15121 ttccatccga tctaaaagag gaggaggaat ggtatccaga acgttggcag tagcaataaa
    15181 cataactcga gaaaggtcga aaggaacacc cagataatgg tctacaaaag cataattttg
    15241 ctccgggtct aaaacttcca gaagagcaga agcaggatct cctctaaaat ccatacctat
    15301 cttgtccact tcatcgagca tgaaaaccgg attattagtt cctgctttcc tgataccttg
    15361 aattattctc cccggcaacg ctcccacata agtacggcga tgaccacgaa tttccgcctc
    15421 atctcttatt cctcccaagg aaatacgcac aaagtttctt cccaaagccc gggcaataga
    15481 tttccccaaa gaggtcttcc ccactcccgg aggaccaaca aaacaaagta tcggtccttt
    15541 aatatctctt tttaacttgt ataccgccag atattctaaa attctttctt ttactttttc
    15601 taaatcataa tggtcttcat ctaatatttt tttagctaaa tctatatcta agttatcttc
    15661 ggtgctaacg ttccagggaa gttcgattat ccagtctaaa tagtttctaa ttatcgggta
    15721 ttcaaaagaa ttaggagaga ggcgggaaag tctctctatt tccttttctg cctctttacg
    15781 tacctcaggg ggcagatcca gactttccaa cttttctctc agttccttaa tttccaggct
    15841 tttttcatct aattctccca gttcttgctg aatggccttt aattgctctc gcagaaaata
    15901 ttctctctga gttttagcca tttcctgctg aacttgagat tgaatgcggc taccaatctg
    15961 cagtatttct aactctcgag tgaggtaaaa gcttaacctg gtaagtttag tagataaatc
    16021 gggagcagcc agaagctcca ttttgctttc cacatccagg ttaagattgg aagctataaa
    16081 attggccaaa cgagaaggtc tcttaatatt tagagctgtt acaaaagctt cacggggaag
    16141 ataaggagct aattccacta cctgctggaa aaggctcacc acatttctta tcagagcttc
    16201 tatctcatca gagtgcactt cttcttcttt tattacctct atcttagcta ccggataagg
    16261 ctcccacctt atccattcct taattcgaaa cttaaccaga ccaagaatta aaacctgtac
    16321 tgcattatcg ggaagtttga taagacgaga gatttttcct ataactccta catcataaaa
    16381 atcttccgga gaaagctttt ctccctttgc ttctcctcgt agcgaaacca ctcccaggat
    16441 ttttttatct aaaaccgcat catttatcag tcttacccat ggttctcccg ttactaccca
    16501 gggcaaagca atttcgggga aaagaacgtt atctttcagg ggtagaatag atatttcttc
    16561 aggaaattca ttttctttgt tttcttctga agaaggagaa tttcccagaa aattagcact
    16621 ttcttcttct tccatcaatc cttcttctaa aataaaccag gcattactcc attccatctc
    16681 tatatctcct gagttatgga aatttttctt tcccgaatat tttttaatcc ttcaatgatc
    16741 aaaaaaccat cggagtattt agctctaact tcctctacct cttctggtaa gttaacttct
    16801 cgatagaaag gaccgaaacg aatctccatc aaatggtaag cctccttaga ggaataaggc
    16861 agctcttttc tttgcccttt cacagtaagc tttttccctt ctattattac ttctacctct
    16921 tgaggagaaa caccggccaa ttctattact atcaccacat gctgaggggt ttcaaaaata
    16981 tcacagtatg gcacccaggt ttcttctcca aattcggtaa atcctacttt cccctcagag
    17041 atatgtttca taaacttctg catctctttt tgaaaataat taatttcttc ccaccaatta
    17101 ttaaagtctt ttttcataag ttcctcctaa aacaccacca ataatctctg caaatgataa
    17161 agtccctgag aagattctac ttctatccgt aataaattat tgcccaactt taaataatca
    17221 gcgatgtaaa tcatctgagc ttcagaggga gaaaccattc cttctgcagc tctacctgaa
    17281 aaaatcaatt ggtcattgag aataatttta agaaatacct catcctgggc tttaccccag
    17341 agatataaag ctcccagatg aagaggctgg gaaagagaaa atcctacctc tatgaattct
    17401 ccatcgagca aagaaagaac ccaggcagga ttaaccgcgt aaccagagag caattccccc
    17461 caataagtta aatgctcaaa attagtataa ggctcaccgg aggtaaagtc gagaaaatac
    17521 aatccctctt taggataaac ttccaatccg cttattgtgc cgtaggcata atcttctgag
    17581 ggaaggagat tcttcacccc ggcgaaagac actttgactt ccaccgtttc ttgccccgcc
    17641 actttaggag catataaacg attggtggtt atacccgcct catcggtgta agttatctct
    17701 tgctcccagc taccaatact cgcttcccac ctgacacttc ttccttctac cggttttccc
    17761 cgcaaatccc ggagttctgc ctgaaaatcg agaacctcac ccccggctat gtgaaaagat
    17821 ttaggagtga gaacaatggt ggtttgtatc ttttcgggga tgaccagaaa acgaatttcc
    17881 ccctctgctt cttcataaat ggtatccccg ggaaaataag cttttaatat cacttctgtg
    17941 ctctcgagag aagatggagc gctataccaa acctcaaccg cacctgaggt agaagtttgg
    18001 gtctcataat tatccagagt tccttgattg acctcccagt aaatcggtcg gttaaataaa
    18061 gcattccctt ctctatcaga caaaaaggct cgtactttta gctttcctcc tgcagggatt
    18121 tcgccgggca cctcgtcaaa agaaatataa gaaggagcta acgttctcct ttcggtcacc
    18181 acttgaattc tacagtaatg caaaagcact ggatacccta caatttcctc tattcgagag
    18241 ggattcctca ttgcctctac cagtaagcga tctggtatta tcttatcaga aagtaccatc
    18301 aagaaccttt ctactccttc tggcccggag accaccccat aaatttttcg ctgtactcct
    18361 tctgctatgg gctcattgtt tttaataatg cgtgctttcc catcaggagt gtagtcaaat
    18421 atgctgacat agccatcttt agagctacca tactgtaaaa caatattttc tccagggtaa
    18481 tagagacccc attctcctcg atcgggaagc aaaaatataa aaggagaaag aggcagggaa
    18541 tcaatttgac ttatctcaaa agaagaaaca tcaaattcaa aagactgagc tccagcccag
    18601 ccactacaaa ggaaaaagat aagtaataga actcctctaa ttttcttcac ttttatccct
    18661 cctcagcttt ttctatactt tcgccactct ccaaaataag agaggtaaaa tcttcctcct
    18721 taatgtctac tccaatacga caatttctac ctactattgc ctcaggggga atgcgagcat
    18781 ttctccctac tatagtcaaa ccacttttga gcatatcagg aattttcttg ttaggacgta
    18841 aatcttcacc atatcctatt ttagctcttt tacccactac tatgcgttta tctaagatag
    18901 cccggtctac cactgcctct tcatctatta aagtatcact cattacaatg gaatggcgaa
    18961 cctctgcacc tctactgaca taaactccac taaagattac cgaatcctcc acactcccgt
    19021 ctatgatggc cccgctacct aacagactcc gtttaattct cgctcgatga ctaagtttga
    19081 ccggcggacg atcttcaatg taagtgtata taggccatcg cttatcgtaa aggtccagag
    19141 cgggcgagga cccgagtagt tccatattag cctcccagta agcatctata gttcccacgt
    19201 ctttccagta accagaaaag caaaatccgt acacttttct ttgtccaatc atcctaggga
    19261 tgatatcttt accaaaatca aaagaagtac cttctcggag agcttcttct tccacttctt
    19321 cttctaaaac gtggcgatcg aagacatata cccccatatt gattaaattg ctacgggggt
    19381 gggggggttt ttcttcaaaa tcaactactc ttcctctctc atcaagagat aacactccaa
    19441 aacgatgagc ttcttcccag ggaacttcca ttaccgctat agttaaatcc gcttttctct
    19501 cctggtgaaa gcgaatcaat tggcgataat ccattttgta tacatgatcc ccggaaagaa
    19561 tgagcaccag ataagggtcc tggtctcgaa taaagcggag gttttgataa acggcatccg
    19621 ccgttcctcg ataccagtgg gtaacgatat cacttatata aggctggaga agaaaaactc
    19681 cgccttccat ccggtctaaa tcccagaact taccaatccc aatatgctcg tgaagagaac
    19741 gaggattata ttgtgtcgga atcccaacac aatatagacc agaattgacg caattactca
    19801 aaggaaaatc aatgatccgg tatttacccc caaaaggcac cgccggttta gctctctttt
    19861 ccgagagaat atccaatctt ttaccttccc ctccagctaa aatcagagct aaaacttctc
    19921 ttttatacac tctttttccc cctttgctaa agcccgcaaa acactatgta ttaaataaag
    19981 aaaatgctgg cgaaagcttt ctttctgaga cgagggatta tcgtctccta accaccagaa
    20041 ccaatcgcta ccttccgcct gatatatgag atcttgtatt ctctctcttt ctccttttga
    20101 taaatattcc ttctccctat cccagagttc cctcacccgc cgcagttctt cccaggccca
    20161 gtttttttct gggtgtccga tccaggtatt tagattgccg cagatccagg aaccaggcac
    20221 aatacgagaa atttgaggaa gatgggaaaa attccccaaa tactcaagag gagtagtagt
    20281 tacaaactcc tcactctcag agagtgtctg atatagagag gtgataaaag gcaacccgtt
    20341 gtctacatag tattcccagg cattttctcc atctaaaatg atatttacca gataaggttt
    20401 atcttcgggg agtgcttctc taatagctct caaatgattc ataaagtctt tcaccgcttc
    20461 ttgaggagga aaatgttgat aactaaaccc gattaagtct gataggccag tatcccggaa
    20521 gaatacagct atctcttttc cgtctttagc ccagacatgg gggagataaa gttcttgcca
    20581 ggctgtttcc tttttcttcc ccaaagaacg aaacaaaatc tcttctcctg tagctaacca
    20641 ttttacgcct tcctggatag ctaaatagat agcttcctca cttaccgctc cctcagaagg
    20701 ccacattccc ataatatcag aaccccatat atcttgagag aaatctttac ccttatttat
    20761 ttgctctcga gcatcttcag ggaaagagaa acgatgaggc aaaatggtag aagggttagc
    20821 ctcccgagct acatccgtat ttataaggag aggtaaaatg gggtgataaa ggggagaaaa
    20881 agaaatttct atctgtcttt tttctaataa tcctcggtag caagcaaaaa tatctttaaa
    20941 tatttcttca gtagctttcc atattttttc tttatcttct tctctataat ttctaccccg
    21001 ctcccataat tctcgaccta ctggattttc ttctcgccac agagggtcaa accagataag
    21061 ttggtagaga acttgaaggt ctaataaatc ctgagaggta aaggactccc agggcttttc
    21121 ttttaactca gcaaaacgag gccacttctc ataggtgcga ggagaagaaa caataaagaa
    21181 tttttctttt attaattttt tatcttcctc gctaatagca gaggctttct ttttagccaa
    21241 tttcattacc aaatcttctt cgccttccag ataaagttga aactgccgaa ggagacaggg
    21301 agcaaagttg aaagtaaccc ggagagaagg aaatttctct accaatttag ccataaaaag
    21361 gtaatctttg gtggcatgaa atctcaccca gggaaaatca aaaactccct ttttttcatt
    21421 ccagtagaag ggctgatgca tatgccaggt aatgttgacc atcaattttt tcgccatttg
    21481 tctcacctca atccactttt aatatttctg cttcaattat tttcccctct ctcaattcca
    21541 taaaaccaaa agagaaataa ggagcaaata cccaatcagt aggactccct ggattaaagt
    21601 ataatacttc tcctcgccat tctttactgg gttgatgagt atgaccgtat actattactt
    21661 ggagattttc cccattgaat aactttccca ctcgctcttc tatccccgca ggagaccctt
    21721 ccccatgagc aattcctatt tttaccccct ctacttctac aatttcttta tcggaaagtc
    21781 tctcccggac cagaggagaa tccatattgc cccttactgc tcgcaccggg gcatacttca
    21841 ttaactcttc cagaaaccac cactctgtta tatctccagc atgaagaatg agagaaactc
    21901 cttccaaaaa acgaagtaaa tagggaggga ggtttctccc tcgctggggg atatgagtat
    21961 cagaaattac tgctattttc atccctcttc accattttta attttatcat ggttttgctt
    22021 tttctaaaac acttcccttc aaaccacact ctttatttca agcattcttt cgctgtaaag
    22081 ctctttctgc tgccttaacc gtattagcca tgagcatggc aatggtcatg ggacctactc
    22141 ctccaggaac cggactgatg taggacgcca cttccttcac tccctcaaaa tctacatctc
    22201 ccacagtctt atcccccact ctattgattc ccacatccac caccaccgcg ccctctttta
    22261 ccatatccgg ggtaattaaa cggggcgagc cacaagcagc aattaaaata tcggccatcc
    22321 gggtataagt tgaaagatta cgagttccgg tatggcacat agagacagtg gcgttagctc
    22381 ctctaccttt ttggactaac atcatagcca ggggtttacc cacaatgttg cttctaccca
    22441 caattactac atttttccct tcgggggaca aattataacg caacaacaat tcttgcactc
    22501 cccagggagt gcagggaagg aaaaccgggt cgccaatcat caatctcccc agattataag
    22561 ggtgaaaacc atctacatct ttttccggag aaatatgata tagaacttct ctctcctgga
    22621 tatgaggagg aagaggaagt tgcaccaata ttccatgaat gcgctcattc tggttcaaat
    22681 tatttactaa ttctatcagc tcttcttgag gagtggtggc aggaagacga tgctgaacgg
    22741 aataaatgcc cagtttttca caggttcttt ccttgtttcg cacgtatatc tggctcgccg
    22801 ggtcatcccc caccagaact accgctacac ccggctgaca accccgctca gtaaggtaag
    22861 caatacgagg tttaagctct tctttaacct ctgccgaagt ttttttccca tctaaaattt
    22921 gagcactcac tttttacctc ctctctctat ttaaaaattt ttctatatta tctctactgg
    22981 agttgagcac ccgttcagga ggaaaaccca cttcctcgag caagccctgg gcttctttga
    23041 attctcccac cgtctcacag aaatgagcat cgctggagac cgctacccat cccccttgct
    23101 tttttacctc ctgagcgatc aagcggcaat tttccctgct gcctcttctg gttattgtaa
    23161 aagagctatt attaatctct ataacctttc ctcgactaac tgctgcttct actacccttt
    23221 catagtccag aggatagcga ggattcccgg gatgagcaat gatatctacc agaggatttt
    23281 ctatcgctcg gagcagcgct tgagtattta tctctcggga agcaggagga aaaatttgag
    23341 gatgaaaaga aacaatcaca atatccagct ttttcaacaa cttttcatcc agtgctaaat
    23401 ttcctttttc atccagtata tctgcctcta ctccaaaata aatttttact cctgctattt
    23461 cccgaggaaa atggccgtgg gattcaaaat aaataggcaa aggaccacca ggcatctcgg
    23521 gactgtggtc agttatcgcc actacctcca gttttttcct ctttgctacc tgcgctattt
    23581 ccataactgt actatatcca tgaccactgg ccactgtatg ggtatgtaga tctcctacta
    23641 aattcaggtt catcacctca ctgtttttta ttagaagcga gcaacctcca tatctggtta
    23701 tcaaactccg ctaccggagg gtcgctccat aagtctaagc ctcgtaacca ggcattatag
    23761 gaaacaagat accgcacaaa agtcatcaac tctcctgttg tttgaaaatc gggtaaataa
    23821 tattcccatt ttagaaaatc ttttccttct ttatacaaca ggttctccac cacccggcac
    23881 tctatctctt ttatttcttt ccaggaacca gaggtaggta ctgacccttc cggagaaaaa
    23941 agatctattc ttttgagagc agaaggaaga gtaaatttga ttttattaat cttttcgctt
    24001 tttaccagga tgaaaataga gttgtcagac agaaataatt gcccatctcg catcatagaa
    24061 aaatcaaaag cgcgaaactc catcttccct tcccctttac aactgtattc cagaagagga
    24121 ataaaacttt gcagaataga aaggataaaa ctattatcta ccaaaaaatt aactctctct
    24181 attccccttt cctctatttc cttgataaaa agagaaagag ctgctgcttc ttcccggaga
    24241 taatcacttt ttttaaatcc ttcctccagc tcctctaccg gcaggttaag actgatcaaa
    24301 ggtagatata gaagagcact cctttgccag aaatagtggt gataaatttc ggtaggtaaa
    24361 aagggaagaa agaggatttg agcactttga gccaatatac catagttact ccctaccagc
    24421 accttttccc actggggaaa agaaaaagct aaaagcaaaa gttgcaactc ctccagtggt
    24481 cgagccagaa ccacaagggc agtatccttg ggaggcaatt tctctttcca gtagagaagg
    24541 agatcaggcc ttgaactgtc aacaagacct ataaactgtt ctccccggga gcttacctct
    24601 tccattagaa aattccggag aaaagaaacc accacaggtg aagataaaaa aatgaggttt
    24661 tttttctccc ttaaaactgc tcgataaaaa ctaaagaatt gctctacaat tacctcttct
    24721 tcctcaaaaa gaagaggtaa tttatctaaa agctcagctc tctcttcctg agagaaaaaa
    24781 cttccctttt ccccctgaga gaaaactaag cttttttttc tcttcgccat aacccatatt
    24841 ttatgctatc agtgagcgct tgccaactag cctcaattat atcagcagac actcccactg
    24901 ttccccagga atctctccca tcagtagatt gaattaaaac tcgtatacga gctgctgtac
    24961 cttctttttc attcaaaacc cgcactttat aatctgacaa ccgaatttct ttcaacaacg
    25021 gatacatctc ttccagcgct ttcctcaaag cattatccag agcgtgaacc ggaccatcgc
    25081 cttctgctgc tgtatgtatc attctctcgt tcactttcac tttaatggtg gcttcggcta
    25141 ctgtatcttc ttctcctctt ttttccacaa ttactctgaa accttccagg tcaaaaaact
    25201 cagcactttc tcctaaagct tctcgcaaca tgagctcaaa agatgcatct gctgcttcaa
    25261 actgatagcc ataattttct gcttccttaa ttttctgtat cagggtaagc aggcgagggt
    25321 ctttaggatc taactctatt cccagctctc tggctcgata agtgagattg ctccttcccg
    25381 cctgttctga taccagaatc cttctctcat tacctaccaa ttcaggagaa gcgtgctcat
    25441 aagtggcagg atagcgagaa atggcactga catgaattcc tcctttatga gtgaaagcac
    25501 ttcttcctac ataggggtca taatcattag gtcttaaatt agctatttca cttacaaaat
    25561 aagaagtctc tctcagtttt cgcatttcct catcagtgat aggatgaata ttcatcttca
    25621 atttcaaatt gggaattatg gtgcagagat tagcattacc acatctttct ccatatccat
    25681 tgatagtacc ctgaacttgc tgtgctccca gatgcactgc tattaagcta ttagctactg
    25741 ctactccact atcattgtga gcgtgaatgc cccactttat cccttcgtct ccaccaaaga
    25801 attccttcat ccggttgatg attacttcca tctctaaagg aagagtccca ccgttcgtat
    25861 cacacaaaac cagaatctgt gctcccgcct catgagcaac ctggagagtt ttcagagcat
    25921 attccggatt atctttaaat ccatcaaaga aatgttcggc atcataaa
//
LOCUS       DAOPSS010000006        25903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_1524_length_25903_cov_9.304085, whole genome shotgun sequence.
ACCESSION   DAOPSS010000006 DAOPSS010000000
VERSION     DAOPSS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 25903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25903
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1524_length_25903_cov_9.304085"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..300)
                     /locus_tag="PKN31_00930"
     CDS             complement(97..300)
                     /locus_tag="PKN31_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98523.1"
                     /translation="MKVRDESLFSVSRKMRSRYSLCVVFALLFRFSSGIVPFSFLLFL
                     AIVSGFILSPGSFLFWGMFSWDS"
     gene            complement(284..1054)
                     /locus_tag="PKN31_00935"
     CDS             complement(284..1054)
                     /locus_tag="PKN31_00935"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOA98524.1"
                     /translation="MSRRWKKMEVPGLEKVLIYETVGSTNDEAKLLAREGYPEGTLVI
                     AEEQTKGRGRKGRDWFSVPGKSLTFSLLLRPPRSTSLALLCALSCAWALEEKGFHVLL
                     RWPNDILLGDKKVGGILVESSFSGDELLCVVVGVGINVNETREDFPPPLRESATSLFL
                     ASSCMWQRGEILRSFLSFFFPVYFAWKERGGDFSPWIEDYEKRSSLLGKEVVVRWGER
                     TVRGVARGVKEDGSLVLANFEGETLVSWGEATLHEGER"
     gene            complement(1014..2129)
                     /locus_tag="PKN31_00940"
     CDS             complement(1014..2129)
                     /locus_tag="PKN31_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOA98525.1"
                     /translation="MVMSNMVKTGSSPVSPPRYDPHRLKKQVLIPVFISVATIAFIFS
                     FSSFRGVTLDLSSFQLLPLGIGLVLVVCAWLIDSFRLFLTIRAWGKKIRFSHALQAML
                     SNYFLSSITPFSTGGSPAQAYVLARRGLTWGEAGSLVTVCGILSQVGLLALLFVFIFV
                     FNIGVRLEGVLLHLLYSFAILYSVVLFLLFFFLHRPSLLYRLTDWGISWVKRTFKRTK
                     FSEEAVRKWVEEFFTDFQRGFSILFLRKPQYLIWNIVCYMFNYLFTFSVAYFVLLSLG
                     VHPSYLEVVGAQIPLYYIFSFIPSPGASGGVEITLASVFLRFAGARRLGMFILLWRII
                     TYYFPMLVGGASFFQLLGEGNNNQNVPAVEKNGSSRA"
     gene            complement(2123..3085)
                     /gene="cbiB"
                     /locus_tag="PKN31_00945"
     CDS             complement(2123..3085)
                     /gene="cbiB"
                     /locus_tag="PKN31_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOA98526.1"
                     /translation="MLGALNLLTGFLLDFFLGDPGGRWHVVVGIGKLIEGLEKALFPQ
                     ERHFRREFVLGFVVVVAMVGGLGVSYFFLRRFLRSYSLFLFSVVEVYLIFNFLSLRTL
                     LRRGEEVKKALDEGDLPRARKSLKHLVGRDTEYLSEWEVVRGCVESLAESFNDGVMAP
                     LFYISLFGALGGLIYKTTNTLDSMLGYRDFRYFFFGFASARFDDILNFLPARLSILFI
                     ACAASVLGRWGRSALSVAFRDARKHESPNAGWPESAMAGALGVRLGGVNYYGGKREET
                     AFLGDFVLDLTPERIGEALSIVRLGSFLALGFFTVISILVGRIW"
     gene            complement(3316..3912)
                     /gene="cobC"
                     /locus_tag="PKN31_00950"
     CDS             complement(3316..3912)
                     /gene="cobC"
                     /locus_tag="PKN31_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HOA98527.1"
                     /translation="MKEIYLLRHGDTDATEKGYFAGWTDVPLSPKGERRINKVREILK
                     GVEFERVFVSPLCRTQETARLVAPSESWEVREEIKERSFGSWEGKSWEDLEKESPEEV
                     KKWREAPSLFVPPGGESFSTILERVVPFWEELLSRPEERVLVVTHGGVIRSIFAYLLE
                     VDFAATYCFLFDPGVLVKTRGNHSFCQVVNILNTEIEP"
     gene            complement(3909..4793)
                     /locus_tag="PKN31_00955"
     CDS             complement(3909..4793)
                     /locus_tag="PKN31_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98528.1"
                     /translation="MRKRAICPGSMGELVQGFREGKEVLISLPIDRYSEMEVIWQEEK
                     REKEKRNILWKSEKALEIALREWGMEHLVGQVAFRRLREIPEGKGLASSTADIAALLG
                     ALARLLGRPLREEEIARIALQVEPTDGVFFSELSVFDHLEGQVKLSLPLPRYLGVVVV
                     ELPGKRDTLTLDREKLRKNWERHRRDVEEAFYLVERGLEEGDLSLVGRGATLSGEIVQ
                     EAEKEDLWEKVISLYREIGAVGVNRAHTGRALGILFDRRVYAPQEMLSRVREALKGEE
                     VKIEVNAVISGGVRVVEL"
     gene            complement(4790..5557)
                     /gene="cobS"
                     /locus_tag="PKN31_00960"
     CDS             complement(4790..5557)
                     /gene="cobS"
                     /locus_tag="PKN31_00960"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOA98529.1"
                     /translation="MAAEIKKAGKETIRRLKMAWGFLTVFPTDTGEYKEGDLSRASFF
                     FPAVGAVIGAVNASLFYLLHFLTRDSLLSAFLVTFIWIFLTRGLHLDGLADSFDALFS
                     GAGKERKQEILKDSHHGTFGVLGILFLVGAKVFLLQGIEKDPLLFLFLAPLLGRVAAL
                     QWGAFFHPFPRTKKGLGEEFVGRIPSFYFFFWLVVAGIMGFMLGGVETLLKIILVFAI
                     IYPLGNRLSGIFGGLNGDLVGLGIELAEILILAGGRF"
     gene            complement(5542..6081)
                     /gene="cobU"
                     /locus_tag="PKN31_00965"
     CDS             complement(5542..6081)
                     /gene="cobU"
                     /locus_tag="PKN31_00965"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOA98530.1"
                     /translation="MQENWDKSVLFLGGARSGKSRLALEWVEESGLSPLYLATGVATD
                     PEMEERILRHQRERSAMWKVEEVPFGLGEALEKDWRGQGVVLDCVTFLVNNIFWKEKD
                     GEETYERLCRELSRIFEKRKKEHFLLLLVSNEVGMGVVPEHPAGREFRDLQGRVNQWI
                     ASQVDEAYLVVAGIPWRLK"
     gene            complement(6062..7096)
                     /locus_tag="PKN31_00970"
     CDS             complement(6062..7096)
                     /locus_tag="PKN31_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA98531.1"
                     /translation="MMTHGGRVIEWQRKSGRTYLDFSANLNPWGPPREVKERWGELFY
                     YATVYPPLDCSFYREELAKIYNLSSSFILPTNGATAGIYLLARQLPGETVLILEPCFS
                     EYARSFSRAGKKIERVFFPVPSNSLPPADIVVWGNPTNPWGVSPPSFLLDYLWEMSLK
                     KGTILVVDEAFQEFIEEKTSWVSRLGENPRLYIIRSLTKYFTLPGLRGGFIAAHPSSC
                     SFLEEYLEPWSINSLLARVLEIIAQGDLASFRATTQENLRREKRFIEENLKGEIGELF
                     PSEVNFYTIRLRQGKELFSSFLEERGILVRSLSDFFGLDDSYFRIAVRTREENQQLIE
                     AVRDYARELG"
     gene            complement(7100..9514)
                     /gene="gyrA"
                     /locus_tag="PKN31_00975"
     CDS             complement(7100..9514)
                     /gene="gyrA"
                     /locus_tag="PKN31_00975"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOA98532.1"
                     /translation="MKETGKIVPVDVNKEMKDAYLSYAMSVIVGRALPDVRDGLKPVQ
                     RRILYAMHEMNLRHNEPYKKSARLVGDVLGKYHPHGDMAVYDALVRMAQDFTYRYPLV
                     DGHGNFGSIDGDPPAAMRYTEVRMSPIASEMLRDLEKDTVDFAPNFDESLREPVVLPA
                     ALPQLLANGASGIAVGMATNIPPHNLTELINACLYLLDHPQASVEELLEIIPGPDFPT
                     YGKIVGREGIRDYFREGRGKLILRGEIEVEEIGRNRMALVVKELPYQVNKANLVENIA
                     RLVDEKKLSEISEVRDESDREGIRIVLELKRGAYPQRVIHHLYKRTALEVSFGVILLA
                     LVDGRPETLGMKEALLCFLEHRRQVILRRSAHDLREGKDRLHILEGFLRALDDIDRVI
                     QLIRSSSRVSEAKERLQKEMGFSEKQAQAILELRLQRLVALEREKILTEYEELKIEIK
                     KLEEILEKEEALKAVIREELEEIKEKYGDARRTRIVEEDGEDIEEVELYPEEEIVVTL
                     TQDGYIKRTSLDSYRRQGRGGKGVTGIVTKEEDAVSQIVVSSTWHRLLLFTSRGRVFQ
                     LLSYHLPDASRSSRGIHLVNLLPLEEGERVFTFISLRDFGAGKHLFFVTAQGNVKKVD
                     LLEFVSITRRGLKAIELKEGDELASVFISGGKDHVLLATARGYGITFSEEEVRSMGRG
                     ARGVRGISLRPGDRVVGAVPLEENKHLVLVSSGGWGKRLDHALLPRQHRGGKGVRIMQ
                     TGKKIGEVVGIALAEEKDEILLSSQEGILIRLSVADIPVQGRTARGARLIRLEEGDRL
                     ASFALS"
     gene            complement(9526..11442)
                     /gene="gyrB"
                     /locus_tag="PKN31_00980"
     CDS             complement(9526..11442)
                     /gene="gyrB"
                     /locus_tag="PKN31_00980"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOA98533.1"
                     /translation="MVEKNNRDYGADNIQVLEGLEAVRKRPSMYIGNVGTEGLHHLVF
                     EVVDNSIDEAVMGFCSRIEVALLKDGSVRVVDDGRGIPVETHGAVGKSAVEVVLTRLH
                     AGGKFDGTAYRISGGLHGVGVSVVNALSEWLEVEIHREGKVWKQRYKRGKPQGEIREA
                     GVSDHTGTVIHFKPDSLIFPDTHFRGDIISQRLKELAFLNPGLTIVFRDETQAEEPEE
                     KIYHFEGGISALVSHLNRGKEVLHPEPIYFSGEKEEIKVEVAIQYNDGYVENILSFAN
                     NIRTDEGGAHVTGFKSALTRAIVHYGEKRGILKDKEEVPMGNDVREGLCAVVNVLIPN
                     PQFEGQTKTKLGNPEARSLVEEVTFSSLSSIFEENPDLARPIMEKVMQTVKARLAAKR
                     ARELARKKDSLDSISLPGKLADCAEKNPEISEIFIVEGDSAGGSAKQARDRRFQAILP
                     LRGKILNVEKANIHRILSNQEIRNIAAALGCGLGFDFSREKLRYHKVIIMTDADVDGA
                     HIRTLLLTFFYRYMKPLVEGGNIYIALPPLYRVREGKNDYYAFDDEELEQVLQGLRGK
                     KYTIQRYKGLGEMNPEQLWVTTMNPETRTLKQVTVQDAIEAEEIFSVLMGDAVEPRRD
                     FIREHAREVVNLDI"
     gene            complement(11455..11766)
                     /locus_tag="PKN31_00985"
     CDS             complement(11455..11766)
                     /locus_tag="PKN31_00985"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOA98534.1"
                     /translation="MEKKEVKSFFGKPTPLGEVLEGFLKKEGLFPQIHSFILLSRFGE
                     WFPHLADFCQPTKLREGVLYLEVNDPLWTLEVKKCIPDILLRCEEEGLEVKKIKIQCR
                     Y"
     gene            complement(11767..12855)
                     /gene="recF"
                     /locus_tag="PKN31_00990"
     CDS             complement(11767..12855)
                     /gene="recF"
                     /locus_tag="PKN31_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889914.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOA98535.1"
                     /translation="MMYLEEVEFINWRNLEKVELSFPEKTLVLAGDNAQGKTNFLEAL
                     FLLARGTSPRRAKDEEMIRWGEEFSYLRAQIREENLHFTREVVIKKEGKKEWKRDGKR
                     ETRRTSLPLVGYFPQDREIVEGPPQKRRDFLDEVISFIYPSYNQWLREYEKLLSRRNF
                     LLRERSSGELVEIYGENLINCGTKVVETRIRYLHRFVPCFQSFYQELSGEDVSLQVFY
                     RPLGYRWEEGVEEGLRRAKKRVREEEKERGVTLFGPHRDEIMFLLRDREARNFASQGE
                     KKRLAISLRLAETSMVESFYRKEVVILVDDVFSELDKKRRALLWQKIKNGSQVFLTTT
                     EEEEVKELRGSSPLSFFRIEGGRIEKGE"
     gene            complement(12855..13943)
                     /gene="dnaN"
                     /locus_tag="PKN31_00995"
     CDS             complement(12855..13943)
                     /gene="dnaN"
                     /locus_tag="PKN31_00995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOA98536.1"
                     /translation="MDIVAKSKEFRKVLGKVYRGVASRPPYPALSGIHLEVENNSLKL
                     TATDLEVGITSRCPVEVREEGSVVLPGKVLYNLVKSLSSPELEIVSLPDGNSEIRGGK
                     SYYKLSGFPADDFPLFPLPSSITSFSVPGEKLKEGLMKVYFTASKDESSLPLSGVLFH
                     FEEEGKLRLVASDDHVLSLCSIEVQNGEVAQYILPLRAITEIIRLAEGETRIFPAKGF
                     ILFQWEDISLFSRLIEGEYPDYKRVIPQSFVTQTSLPGDELRKALERVSLVAPPESPV
                     VELLLGEGKIELSSSGEVGIAKEEVEAEIEGEEIKIFFNARYLLDTLRSFSGEKAMLE
                     ISGELAPTRLRGEDEHLQYILMPVEIKE"
     gene            complement(14127..15485)
                     /gene="dnaA"
                     /locus_tag="PKN31_01000"
     CDS             complement(14127..15485)
                     /gene="dnaA"
                     /locus_tag="PKN31_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOA98537.1"
                     /translation="MKVSPEELEDLWIKTLSLLEKQVSVPEYRAWVENIQPLSLLDHR
                     LTIAVPSELSKEKIEKKYVETIRYAFQKVAGVNFPNLEIGLVVKPSSTSLGEEVSSFS
                     SSVAGEDRLSLNPRYTFSTFIVGNSNKFAHAAAMAVAENPAHSYNPLFIYGGVGLGKT
                     HLMHAIGHHILSNNPQARIVYASSEKFTNELINSIRDDQTEAFRQKYRHVDILLIDDI
                     QFLAGKERTQEEFFHTFNTLHDAFKQIVITSDRPPKEIPTLEDRLRSRFEWGLIADIQ
                     PPDLETRIAILSKKAELEKFELPSDIIYFIAMNIPSNIRELEGALIRIKAFCSLNKIT
                     PTLEVAQEVLKDLLPRQETSSIPISLIQKVVAEHFNIKPALMKAKKRTKEVAYPRQIA
                     MYLARELTNYSLPSIGEEFGGRDHTTVLYACEKIKGDIESNPQLFQEISELIEKIKRE
                     KS"
     gene            15863..18454
                     /locus_tag="PKN31_01005"
     CDS             15863..18454
                     /locus_tag="PKN31_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOA98538.1"
                     /translation="MQIKTKISLGFIVLTLAALAIGVIAIYAFWEINTSLEEAQSYNP
                     LLLVTSRLKDLISQNNSLISSYFREEDVENLNQIEKGFTEVNNRFLVYLEALRLGTES
                     EDFQKWHYGLWEKENFPYALSSLPEDSPLFQEVQELKSLQVNYQARVNMVKTLWKQYL
                     TTQKERNQKSLEMDEPYQIVVQFTQLVDDSIKQLMNPLDSTYYLLFRYAVNGDPDGRL
                     GENINAYFASLRRGIEDSQVLSEESKKSITAQVEDLAGKWEELEKVLFTEAEKDDKFM
                     EVYRSYSRLKTTIEGLRLDNWIKLMSDINQNQKNYLLASDPVEKEAIKKKVNGLMDTL
                     GQFLEGDFLKIYATNTAQVVVNDRWKPFRNLWQEVVKGDEQLRGLNETIQTSLSDVWD
                     VGEDLSQAMETMNQEVLSSFDQALLRARELQNRLSQILYIVIGVVIVFSLIMGTTLSR
                     SITRPIQESVAFAEVLAEGDLTQEITKKRKDEMGKLFASLNQASSSLRRFLQEVREGA
                     DKIISSLDALQHTSREIREAGEQIAQTVSQVARGSEEQSQNLTEVSNHMESLVEEVKE
                     MAKRLKEQADKARQTLQETDKVIESLEVTGGNIERVKGAASSAFQATEEGEKTLEEVV
                     EAMGRIKDSVFSVGEVVKNLGVSSQEIGSITDLITGIAEETNLLALNAAIEAARAGDA
                     GRGFAVVADEVRKLAEESAHAAQRIAHLISEIQKEAERAVRSMTESQTHVEGGSEAVD
                     KAREAFQDIHEANQVVTQEADNISLSFQVVEEASQHIIQLVREVNDIAKESEEGTGRI
                     VERAEEVFTRLSSVASISEENAAAAEEVAASSQEQNAALQEVDVTIQELGKMAEGLKE
                     NLGQFKI"
     gene            18576..21746
                     /locus_tag="PKN31_01010"
     CDS             18576..21746
                     /locus_tag="PKN31_01010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOA98539.1"
                     /translation="MRGEKKLPILLILIFLLFFSFPGEAKRALLVGVCDNPPLVFRDE
                     KGRVRGMCPEVLEHIAQREDWDIEYVFLPFSLLLSHLAEGKIDLLLPIAYTAERAEHF
                     DFNQQTFLVNWGEVYTLPAVSVQSLLDLASRKVAVVKDDVYGESLKKLLRDFGVECYF
                     LEMENYPGVFAAVKEGRADAGVVSRLYGAQFARAAGLAATSIVLSPVELRFAVPRGKN
                     RGIIDIIDQHLLALKINPQSVYYQSLSSWLSVEEEGWRFPSWFWWFLGVVLLFLCLSL
                     FLSYFLRREVGRRTKELVEKSEKLAEEIERREALEEELRFGLDFQKLVAGVSFHFVTA
                     SSFEEAVRFFLEEVRRFSRADEAAFFGEEEGYLGREGNTEKVEIEEGMVLWRERLCLE
                     GPVWTEGESLPERCWRKKEPGEISFLLSFPLEIENRCLGFWELLWREKGNTDKTWIEE
                     HLDLFQVFSYLLSNALEREKTRKDLQRERGWLETILRSIADGVIVTDLEGLVSFMNRE
                     AEALTGWSLGEARGKKLEEIFPLREKNSGKPVENLVEEVLKKGKTEMKEPLLLRNREG
                     KEIVVADSGASVGREGELQGVVFVFRDVTERQRMEEALRRSERRYRLLFNQMLDGFAL
                     LEEIYDGRGEPSYEFVFLEANPAFEHITGFSREDVVGLTLREVFPFLDDSYWELFHQV
                     AEKEEAARVERFVSYKDKYLEIVAFSPSPGQVATIWRDVTEKRKREEEMRYLSFHDVL
                     TGLYNRAFFEEEIQRLDRGRHLPLSLIFLDVDGLKEVNDSLGHEEGDRLLGEIAQVLR
                     KSTRGGDVITRWGGDEFIVLLPQTTEDIAREVGKRIEKNCEEAQRECLLPLSLSWGVA
                     TKESDTEDIREIFRRAEEEAYQKKFLTRKVFLRSLISILVHRFYEEEKIRQRQMRVGE
                     LIQKMGEKRGWEEEEKKKLHFLNLLWVWEKMFLPEHLKEDGYPLLREELVEIYPEFEE
                     QLPLSYSGWSSLFSRVLSGEEYYPGEGDEVPLMARLLSVVEAYEFLVEERQLTPQEAV
                     REIQKEAGSRFDPELVAILEEIVREE"
     gene            21957..22091
                     /gene="rpmH"
                     /locus_tag="PKN31_01015"
     CDS             21957..22091
                     /gene="rpmH"
                     /locus_tag="PKN31_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOA98540.1"
                     /translation="MKRTYQPHRVPMKRTHGFRARMKTKGGRQVLSRRRKKGRKRLTV
                     "
     gene            22095..22445
                     /gene="rnpA"
                     /locus_tag="PKN31_01020"
     CDS             22095..22445
                     /gene="rnpA"
                     /locus_tag="PKN31_01020"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOA98541.1"
                     /translation="MEGLSQKKDFSRLFSRGKRARANNIKLWYLQKEEGNLRVTFVGK
                     GKKAVDRNRVRRRLREAFRVYYYPQWRDKPVDFLFMSNEKLLEESFEDLVEMMGKVLG
                     EVKLDGVKTKSEAE"
     gene            22432..22659
                     /gene="yidD"
                     /locus_tag="PKN31_01025"
     CDS             22432..22659
                     /gene="yidD"
                     /locus_tag="PKN31_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOA98542.1"
                     /translation="MKLSSLVVSGLDKILLFYQRYISPLFPPCCRFEPSCSEYARQAL
                     CKYGPWKGGLLALRRLLRCHPCSPGGYDPVK"
     gene            22680..23462
                     /locus_tag="PKN31_01030"
     CDS             22680..23462
                     /locus_tag="PKN31_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOA98543.1"
                     /translation="MFSQLLDVLAQAMEYLLRFFYNWTGNYGISIILLTLFIRLILSP
                     LIHKQNLSTRKMQEDMQELQPEIKKLQEKYGNNSQKLNEEMMKLYKEKGINPLGGCLP
                     GCLPLLIQLPILMVLYRVLMAYDYGQAGFLWLPSLSQKDPYFILPLLMGVTTFWQQRI
                     SMPPAAEGAQQQNAAMMVVMPIFLVFISWNLPSGVLLYWFVSNLFYILQQYVLNNQIQ
                     RSKSLSIEPEISVKAEPAVSLKDPSSAAPVAKKGGKINAKKR"
     gene            23446..24201
                     /gene="jag"
                     /locus_tag="PKN31_01035"
     CDS             23446..24201
                     /gene="jag"
                     /locus_tag="PKN31_01035"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOA98544.1"
                     /translation="MPRSVEVSGKSLEEALERAARYFDVLPESLSYEVISEGRGFLSA
                     FTPRVVKVRVWIEETPQERIPGEEKSPGKEATEEAYPSPASLLRAEEELGKFFKELMD
                     KMQMEVDFQVQEKDGFLSLFVEGKDAGMLIGKRGETLEALETLLRIVMSKKGFSNVGL
                     QLDISGYRERREEALRRLAEKSAKKVIKEKRRLKLEPMNARERRIIHTSLKDYPEVTT
                     YSVGEGTGRRVVVDLKKEKSSNRSGGNKRPVGK"
     gene            24170..25564
                     /gene="mnmE"
                     /locus_tag="PKN31_01040"
     CDS             24170..25564
                     /gene="mnmE"
                     /locus_tag="PKN31_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOA98545.1"
                     /translation="MEEIKDLLVSDTIVAPATPLGVGAIGIVRMSGREAAEIASRLFV
                     SRCGRAVEDFVSHRLYLGDVFDPQTQEIVDEALLVVMRAPHSYTREDVVEFQLHGGPV
                     VIQKVLQLILREGARLAEPGEFTRRAFLNGRIDLSQAEAVAGIVKARSEKALSLSLRN
                     LKGEWGRKIRSWEDRLLSILALIHAHCDFLDEEELPLEAMEKELASLIEEIEKELSSG
                     ERLKLAQEGALVAIVGKPNVGKSSLFNAIVGWDRAIVTPFPGTTRDALEENILVDGLL
                     LRLVDTAGMREGRDPVELVGVQKAEEYLEQADFVVVVLDQSIPLEKEDEVLAEKVRNK
                     PHFVVLNKSDLLPQLNKSDVSLLYPGEEILEVSALQGEGIEKLLRKIGERIKAEIGPE
                     DQTILITARQREGLKKALLSLVQARDSLREGMSLDVLGLDLEEALREMGRILGTQADK
                     ELLNTIFSQFCLGK"
     gene            complement(25722..>25903)
                     /locus_tag="PKN31_01045"
     CDS             complement(25722..>25903)
                     /locus_tag="PKN31_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98546.1"
                     /translation="RSIQGQAKNGSKVAGVTKKGRWNHSEVTPLSSCLKFSEASTSSV
                     IPEISSRESKFLLFF"
BASE COUNT         7268 a         6148 c         5785 g         6702 t
ORIGIN      
        1 ctccaccaca gataaaaaaa cctggcaaaa atccaaaagg gggattaaga agataagtta
       61 accctccaaa ggagggagaa gcaaaaaccg gaaagcctac gagtcccagg aaaacatacc
      121 ccagaaaaga aagcttcccg gagagagaat aaagccactc acaatagcta aaaaaagaag
      181 gaagctaaaa gggactatcc ctgaagaaaa gcggaacaaa agggcaaaaa ccacacacaa
      241 agaatagcga gagcgcatct tacgagaaac cgagaagagg ctttcatctc tcaccttcat
      301 gaagcgtggc ttctccccag gaaacgaggg tttctccctc gaaattagcg agcacaagag
      361 agccgtcctc tttaacgccc cgcgccactc ctctaacagt cctttctccc cagcgcacca
      421 ccacttcttt tcccagaaga gaagagcgtt tctcatagtc ttctatccag ggagaaaaat
      481 cccctcccct ctccttccag gcaaaataga cagggaagaa aaaggaaaga aaagacctca
      541 aaatctctcc tctctgccac atacaagaag aggccagaaa aagagaagtg gcgctctccc
      601 gcaaaggagg agggaaatct tctctcgtct cattcacgtt tatccccacc cccacgacca
      661 cacaaaggag ctcgtccccg gaaaaggagc tctctaccag aatccctccc actttcttat
      721 ctcctaaaag gatgtcattg ggccagcgaa gaagtacgtg aaagcccttc tcctccagag
      781 cccaggcaca agaaagggcg caaaggagag ctaaagaggt gctccgggga ggacgaagga
      841 gaagagaaaa agtgaggctt ttccccggaa cagaaaacca gtctcttcct tttctgcccc
      901 tgccttttgt ttgctcttct gctataacga gcgtcccctc aggatagcct tcccgggcaa
      961 ggagcttcgc ctcgtcgttt gtagaaccaa cagtctcata gattagaact ttttcaagcc
     1021 cgggaacttc catttttttc caccgccggg acattctggt tgttgtttcc ctctcctaaa
     1081 agctggaaga acgaagctcc tcctaccagc ataggaaaat aataggtgat gattctccat
     1141 aagagaataa acatccccag gcgccgagca ccagcaaacc gcaaaaaaac cgaagccagg
     1201 gtaatttcca cgcccccgct agctccggga gagggaataa aggagaaaat gtaataaaga
     1261 ggaatctggg ctcccaccac ttctaaataa gaagggtgta cccccaaaga aaggaggaca
     1321 aaataggcaa cagaaaaagt aaataaataa ttgaacatat aacaaacgat gttccagatt
     1381 aaatactggg gttttcttaa aaagagaatg ctaaatcccc gctggaaatc ggtgaagaat
     1441 tcctcaaccc actttctgac cgcttcttca gaaaattttg ttcgcttgaa agttcttttt
     1501 acccaggaaa ttccccaatc ggtcaggcga taaaggagag agggacggtg taaaaagaaa
     1561 aaaaggagaa ataaaaccac actatacaag atagcaaaag aatagagaag gtgaagcagc
     1621 accccctcca ggcgaactcc gatgttaaaa acgaagataa agacgaaaag caaagccaat
     1681 aaacccacct gagagagaat gccacaaacc gttaccaggc tccccgcctc accccaggtt
     1741 aaccctcgac gagctaaaac ataagcctga gccggactac ccccggtaga aaaaggagtg
     1801 atagaggaca aaaaataatt ggaaagcatg gcctgaagag catgggaaaa gcggattttt
     1861 ttcccccagg cccgaatagt gagaaaaaga cgaaagctat cgatgagcca ggcgcataca
     1921 accaacacca ggcctatccc taaggggaga agctgaaaag aagaaaggtc gagagtaacc
     1981 cctcggaagg acgaaaaaga aaaaatgaaa gcaatggtcg ccacacttat aaacaccgga
     2041 atcaatacct gctttttcag ccgatgaggg tcatagcggg gaggggaaac aggggaagaa
     2101 ccagttttaa ccatattgct catcaccata tcctgcccac caaaatggaa attaccgtaa
     2161 aaaatcccag agccagaaaa gaacccaggc gcacaatcga cagcgcttct ccaatccgct
     2221 cgggagtgag gtctaaaaca aaatccccca gaaaagcagt ctcttctctt tttcccccgt
     2281 agtaatttac ccctcctaac ctcaccccca gagcaccagc catagcactc tctggccacc
     2341 ccgcgttggg gctttcgtgt ttgcgagcat ctcgaaaagc tacagacaag gcacttcttc
     2401 cccagcgacc caaaacagag gcagcgcaag caataaaaag aatgctcaag cgggcgggaa
     2461 ggaagtttaa gatgtcgtca aatcgagcgg aggcaaatcc aaagaaaaaa tagcgaaaat
     2521 ccctgtagcc cagcatggaa tccagagtat tggttgtttt gtaaatcaaa ccccctaagg
     2581 cgccaaatag agaaatataa aagagagggg ccatcactcc atcgttaaag ctttctgcta
     2641 aagactccac gcaccccctt actacctccc actctgaaag atactcggtg tctcttccca
     2701 ccagatgctt taagcttttc cgcgcccggg gcagatcccc ctcatccaga gccttcttca
     2761 cttcctctcc ccgacgcaat aaggtgcgca aagataaaaa attaaagatg aggtaaacct
     2821 ccaccaccga aaagagaaac aaagaataag aacggagaaa acgacggaga aaaaagtagc
     2881 ttactcccag cccccctacc atcgccacca ctaccacaaa acccagaaca aattctcgcc
     2941 ggaagtggcg ttcctgggga aaaagagctt tttctaagcc ttcaattaat ttaccaattc
     3001 ccaccaccac gtgccatctt cctcctgggt ctcccagaaa aaaatctaaa aggaatcctg
     3061 tcagcaaatt caaagcgccc aacattaggg ctccacctca aaatatctat gattaaccac
     3121 agaaatcatt gtaacttcct ccctaaaaag aaagcgcttt cttcacaccg ccgccgcccc
     3181 ggcgtagttt gttttaattt ttcttatctt ttttgtgcct tttctacttg ccgcaagctg
     3241 ttttgtaccc cctcgcgata gcggctacct cgcacaatag aaaagggaga ggctgccatc
     3301 catcaaagcg ctcgcctacg gctctatctc ggtgtttaat atgttcacca cctgacaaaa
     3361 agaatggttt cctctcgttt taacgaggac tccagggtca aaaagaaagc aataagtggc
     3421 cgcaaagtcc acttccaaaa gataagcaaa tatagagcga atcacccccc cgtgggtaac
     3481 cacaagcact ctttcttctg gtctactgag gagttcttcc caaaaaggaa ccactcgctc
     3541 cagaatagtt gaaaaacttt ccccgccggg aggaacaaag agagaaggag cctctctcca
     3601 ttttttaact tcctcggggg attctttttc caaatcttcc cagctttttc cctcccagct
     3661 tccaaaagag cgctccttga tttcttctct tacttcccag ctctcagaag gagcgaccag
     3721 ccgggcggtc tcctgagtac gacagagggg gcttacaaaa actcgctcaa actccactcc
     3781 ttttagaatt tctctcactt tattaattct tctttcgccc tttggagaga gagggacgtc
     3841 ggtccagcca gcaaaatagc ctttttccgt agcatcggta tcgccatgac gaagcaaata
     3901 aatctctttc atagttccac caccctcact cctccagaaa taactgcgtt aacttctatt
     3961 ttcacttctt ccccctttag cgcttccctt acccgagaga gcatctcctg cggagcatat
     4021 actcttcggt caaataagat tcccaaggct ctcccggtat gagcccggtt tacccccaca
     4081 gcacctatct ctcgataaag agaaatcacc ttctcccata aatcttcttt ttccgcttcc
     4141 tgcacaatct cgccacttaa agtggctcct cgccccacca aagagaggtc tccctcttct
     4201 aatcctcgct ccacaagata aaaagcctct tctacgtccc ggcggtgcct ctcccaattc
     4261 tttcgcagct tttctctatc cagcgttaaa gtgtctctct ttcctggtag ttccaccacc
     4321 accactccca gataacgagg caggggcaaa gataatttaa cttgtccctc taaatggtca
     4381 aaaacagaga gctccgagaa aaaaacacca tcggtgggct ccacctgcag cgctattctc
     4441 gcgatttctt cttctcgaag aggcctcccc aggagtcgtg caagagctcc taaaagagca
     4501 gcaatgtcag cagtgctcga ggccaggccc ttaccctccg ggatttctcg cagacggcga
     4561 aaagcgacct gtccgaccag gtgttccatg ccccattctc tcagggctat ttccagcgct
     4621 ttttcgctct tccacaggat gtttctcttc tctttctctc tcttttcttc ttgccagatt
     4681 acctccatct cgctgtagcg gtcgatgggc aaagagatga gaacctcctt cccctctcga
     4741 aatccctgta ctaattcccc catgcttcct ggacaaatag ctctttttct caaaatctac
     4801 ctcctgctaa aatcaaaatt tctgccagct caatccccag acccacaaga tctccattta
     4861 gccccccgaa aattccactc aatctgttac ccagaggata aataatagca aagacgagga
     4921 taattttcaa aagggtctcc actcctccca gcataaaacc cataattcct gcaacgacga
     4981 gccagaagaa aaaataaaaa gagggaattc gccccacaaa ttcttccccc agacctttct
     5041 ttgtgcgagg aaaagggtgg aaaaaagctc cccactggag agctgccact cttcccagaa
     5101 gaggtgcgag aaacaaaaaa agaagagggt ctttttctat tccctgcaaa agaaaaacct
     5161 tggctcccac cagaaataag attcccaaca ctccaaaagt accgtgatgg gaatctttaa
     5221 gaatctcttg cttccgctcc ttgccggcac cagaaaaaag agcatcgaaa gaatcggcca
     5281 ggccatccag gtgtaagccc cgggtgagga aaatccagat aaaagtcact aaaaaagcgg
     5341 aaaggagaga atcccgggtt aaaaaatgga gaagataaaa aagcgaagca tttactgccc
     5401 cgatcactgc gcccaccgcg gggaagaaaa aactggcccg agaaaggtcg ccttctttgt
     5461 attctccagt atctgtggga aaaacggtga gaaagcccca ggccatcttt aaccgtcgta
     5521 tcgtctcttt gcctgctttt tttatttcag ccgccacggt atccctgcca ccaccaaata
     5581 tgcttcatcc acctggctgg ctatccactg attaaccctt ccctgtagat ctcggaactc
     5641 tcttccggcg gggtgttctg gcaccacccc catccccacc tcattgctca ctaaaagcag
     5701 caaaaagtgc tctttttttc ttttttcgaa tatacgcgat agctccctac acaggcgttc
     5761 ataagtttct tctccatcct tttctttcca gaaaatattg ttcaccagga aagtgacgca
     5821 atccagcacc accccctgcc ccctccagtc tttttctaaa gcttctccca ggccaaaagg
     5881 aacctcctct actttccaca tcgcggagcg ctctctctgg tggcggagaa ttcgctcttc
     5941 catctccgga tcagtagcca ccccagtagc taaatacaaa ggagaaaggc cactttcttc
     6001 tacccactca agggccaggc ggctttttcc gctccgtgct cctcctaaaa atagtacgct
     6061 tttatcccag ttctcttgca taatctctca ccgcctctat taattgctgg ttctcttcgc
     6121 gagttcgcac tgctatgcgg aagtagctat catctaaacc aaaaaaatcg gaaaggctcc
     6181 tcaccaatat ccctcgctct tctaaaaaag aagaaaaaag ttctttaccc tgtctcaatc
     6241 gaatggtgta gaaattaact tccgagggaa aaagttcccc gatctcccct tttaagtttt
     6301 cttctataaa tcgcttttct ctccggaggt tctcttgcgt ggtagctcga aaagaggcca
     6361 aatccccttg agcaatgatt tctaacaccc gggccaaaag ggagtttatg ctccagggct
     6421 ccagatattc ctccagaaaa gagcaagaag aaggatgggc agcaatgaac cctccccgca
     6481 aaccggggag agtaaaatat ttggtaagag agcgaatgat ataaagacga gggttctccc
     6541 cgaggcggga aacccaggag gttttttctt ctataaactc ctgaaaggct tcatccacaa
     6601 caagtattgt tccttttttt aagctcatct cccacaaata gtctaaaaga aaagaaggag
     6661 ggctcactcc ccaggggttg gtggggtttc cccacaccac gatatctgct gggggaagag
     6721 agttagaagg aacggggaaa aataccctct ctattttttt tccggcacgg gaaaaacttc
     6781 gggcatactc tgaaaaacag ggctctaaga tgagaaccgt ctctccgggg agctgacggg
     6841 ctaaaagata aattcctgcc gttgcgccgt tcgtgggcag aataaaagaa gaagaaagat
     6901 tataaatctt ggccagctct tctctataga aggagcagtc aagaggaggg taaactgtag
     6961 cataataaaa aagctctccc catctctctt ttacctcccg gggaggaccc caggggttca
     7021 agttggcaga gaaatccaag taggttctac cgctttttct ctgccactca ataactcttc
     7081 ctccgtgcgt catcatcaat caactcagag caaaagaagc aagacgatct ccctcctcga
     7141 ggcgaattag cctcgctccc cgggcagtgc gcccctgcac cgggatgtca gccactgaga
     7201 ggcgaattaa aattccttcc tgggaggaga gtaatatttc atccttttcc tctgctaaag
     7261 cgatgcccac cacttcgccg atttttttac cggtctgcat gattcttacc ccttttcctc
     7321 ctcggtgctg gcgaggaagc aaggcgtggt ccagtctttt cccccatcct ccactcgaga
     7381 cgagaaccag gtgtttattt tcttccaggg gaaccgctcc caccacccga tctccagggc
     7441 gcagacttat ccctctcacc cctcttgctc ctctacccat agaccttact tcttcctcac
     7501 tgaaagttat tccataaccc cgggcagtgg ctaaaagcac atggtctttc cctccagaga
     7561 tgaaaacgga agccagctcg tccccttctt ttagttctat agctttcaat ccccggcgag
     7621 tgatggagac aaattccagc aagtccacct tttttacatt gccctgagcg gtaacaaaga
     7681 aaagatgctt tcccgccccg aagtcgcgca aagaaatgaa agtaaacacc cgctctcctt
     7741 cttccagggg aagaagattg acaagatgaa ttccccgaga agagcgagaa gcatccggta
     7801 gatggtaaga aagaagctgg aaaactcttc cgcgactcgt gaaaagcaaa agccgatgcc
     7861 aggtgctaga gaccactatc tggctaacgg catcctcttc tttggttacg ataccggtaa
     7921 ctcccttccc ccctcttccc tgacggcgat aagaatctaa agaggttctt ttgatgtatc
     7981 cgtcttgagt gagggtgaca actatttctt cttcagggta cagctccacc tcttctatgt
     8041 cttctccatc ttcttctacg atacgggttc tcctcgcatc tccgtacttt tctttgattt
     8101 cttccagctc ttccctgata actgctttca atgcctcttc tttttctaaa atttcttcca
     8161 gtttctttat ctctattttt aattcctcat attcggtaag gattttttct ctctctaaag
     8221 ccaccaggcg ctgcaagcgt agttccagga tggcctgagc ctgtttttca ctgaagccca
     8281 tctccttctg taatctctct ttagcttcag aaacgcggga agaagagcgg atgagctgaa
     8341 tcacccggtc aatatcgtcc agagctctca agaacccctc cagaatgtga agccggtctt
     8401 tcccctctct caaatcgtga gcgcttctgc gcaaaattac ctgacggcgg tgctctaaaa
     8461 aacagagcaa agcctccttc attcccaggg tttccggacg tccatctact aaagcaagca
     8521 gtatcactcc aaagctgacc tccagcgccg tacgcttgta aaggtggtga atgacccgct
     8581 gaggataagc ccctcttttc agctctaaaa ctattcttat tccctcccgg tctgattcgt
     8641 ctcgaacctc cgaaatctcg ctgagtttct tctcgtccac caggcgtgca atgttctcca
     8701 ccaggttggc tttgtttacc tgataaggca gctctttaac cacaagggcc atcctgttac
     8761 gacctatctc ctctacttct atctcccctc gcagaataag ctttcctctt ccttctctaa
     8821 aataatcacg aatcccctct cttcccacta tttttccata ggtgggaaaa tccggtccgg
     8881 ggatgatttc tagaagctcc tctactgaag cctgtggatg atctaaaaga taaagacagg
     8941 cgtttatgag ttctgttaaa ttgtgaggag ggatattggt ggccatgcct actgcaatac
     9001 ctgaggcgcc attggccaga agttgaggga gggctgccgg taaaaccacc ggctcccgga
     9061 gtgactcatc aaaattggga gcaaaatcta ccgtatcttt ttctaagtcc cggagcattt
     9121 ctgaggcaat aggagacatc cggacctcag tatagcgcat ggctgctggc gggtctccat
     9181 cgatggagcc aaagttgcca tgcccgtcta ctaaaggata acggtaagtg aaatcctgcg
     9241 ccatacgcac taaagcatca taaaccgcca tgtccccgtg ggggtgatat ttacccagca
     9301 catctcccac caggcgggcg ctttttttat aaggctcgtt atggcgaagg ttcatctcgt
     9361 gcatagcata gaggatacgc ctctgtaccg gttttaagcc atcccgcaca tcggggagag
     9421 ctctccccac aatgacactc atggcatagc tcaggtaagc atccttcatt tccttgttta
     9481 catctactgg aacaattttt cctgtttctt tcataaacta tctccttata tgtccaggtt
     9541 taccacttcc cgggcatgtt cccggataaa gtcccgacga ggttctactg catctcccat
     9601 caaaacacta aaaatctctt ccgcttcaat ggcatcctgt accgtaactt gctttaaagt
     9661 acgagtttct gggttcatag tagtaaccca tagctgttcg gggttcattt ctcccagacc
     9721 tttatagcgc tggatggtat attttttccc tcgcaaacct tgcaagacct gctccagctc
     9781 ttcgtcatca aaagcataat agtcgttttt cccctctcgc actcgataaa gaggaggaag
     9841 agcaatataa atgtttcctc cctctaccag aggtttcata taccggtaga aaaaagtaag
     9901 aagaagagtg cgaatatggg ctccgtctac atcggcatcg gtcataatga taactttatg
     9961 ataacgcaat ttttcccggg aaaagtcaaa acccagccca caacccagag cagcagcgat
    10021 attcctgatt tcctggttag agaggatgcg gtgaatgttg gctttttcta cattcagaat
    10081 tttaccccgg agaggcaaaa tggcctgaaa cctcctgtcc cgggcctgct tagctgagcc
    10141 tcctgccgaa tcgccctcca cgatgaaaat ttccgatatc tcggggtttt tctctgcaca
    10201 gtcggccagt ttcccgggaa gagaaataga atccaggcta tcctttttcc gagccagttc
    10261 ccgggcgcgc ttcgcggcca ggcgagcttt tacagtctgc atcacttttt ccataatagg
    10321 gcgggcgagg tctggatttt cctcaaaaat agaagaaaga gaggaaaaag taacttcctc
    10381 caccaggctc ctggcttcag ggttacctaa tttggtttta gtctgccctt caaattgggg
    10441 gttgggaatt aaaacattga ctactgcaca cagcccttct cgaacatcgt ttcccatggg
    10501 gacttcctct ttgtctttca ggatgcctcg cttttctcca tagtggacaa tcgcccgggt
    10561 aagagcgctc ttgaatccgg taacgtgggc tcctccctca tcggtgcgaa tgttgttggc
    10621 aaaagagaga atgttctcca catatccatc gttgtactgg atagctacct ccacctttat
    10681 ttcttctttt tctcccgaaa aataaatggg ctcaggatgt agcacttctt taccccgatt
    10741 gagatgagag actaaagcag aaatccctcc ttcaaaatgg taaatctttt cctcgggctc
    10801 ctcggcttgc gtctcatcac ggaaaacgat agtcagcccc gggtttagaa aagccagctc
    10861 tttaagccgc tgagatatta tatctccccg gaaatgagta tcagggaaaa tcaaagagtc
    10921 gggcttgaag tgaatcaccg ttccggtgtg gtcgctgacc cctgcttctc ttatctctcc
    10981 ctggggtttc ccccttttat atctctgctt ccacaccttc ccctcccgat gaatttccac
    11041 ttccaaccac tccgaaagag cattaactac tgatactccc actccatgca aacccccgga
    11101 gatccgataa gccgttccat caaattttcc cccggcatgc aggcgagtta aaaccacttc
    11161 caccgctgat ttacctactg ccccgtgtgt ctctactggt atacctcgcc catcatctac
    11221 cactcttacg ctaccgtctt tgaggagagc tacttcaatt cggctacaga agcccatcac
    11281 tgcctcgtca atactattat ctaccacctc aaacacgagg tggtgcaacc cctctgttcc
    11341 tacattacca atatacatgc tggggcgctt tctaaccgcc tccaatcctt ccaaaacctg
    11401 gatattatcc gctccgtaat ctcgattgtt tttctctacc aaaactttat cacctcaata
    11461 gcgacattga attttaattt tttttacctc taaaccttcc tcctcacagc ggaggagaat
    11521 gtccggtata cattttttca cttctaaagt ccacaggggg tcattcactt caagataaag
    11581 aactccctcc cgcaacttgg tgggctggca aaaatcagct aaatggggaa accactcccc
    11641 aaaacgagaa agcaaaataa aactgtgtat ttgaggaaag agcccctctt tttttaagaa
    11701 accctccagc acctctccta aaggagtagg cttaccaaaa aaagacttaa cctctttttt
    11761 ctccatttat tccccttttt caatccttcc cccctctatc cggaaaaaag agagggggct
    11821 agaacctctc aactctttta cttcttcctc ctcggtggtg gttaaaaata cctggctccc
    11881 gtttttaatt ttctgccaca gaagagctct ccttttttta tccagctccg aaaaaacatc
    11941 atctactaat attaccactt cttttcggta aaaagattcc accatagaag tttctgccag
    12001 gcgtaaagaa atagccagcc tctttttctc tccctgcgag gcaaaatttc gagcttctct
    12061 atctctgagg agaaacatta tctcgtcccg gtgagggcca aaaagagtca ctcccctttc
    12121 tttttcttct tctcttaccc tttttttggc cctgcgtagg ccttcttcca ctccctcttc
    12181 ccaccgataa cccagaggac gatagaacac ttgaagtgaa acatcctctc cggagagctc
    12241 ctggtagaaa ctctgaaaac aaggaacaaa ccggtggagg tatcgaatgc gggtttccac
    12301 cacttttgtg ccgcaattaa ttaaattttc tccatatatt tcaacaagct ccccggaaga
    12361 gcgctcccgg agaagaaaat tacgacggga gagaagtttt tcatattctc tgagccattg
    12421 attgtaagag ggataaataa aagaaataac ctcgtccaga aaatctctcc gcttttgagg
    12481 aggaccttcc acaatttccc ggtcttgagg gaaatagccc acgagaggaa gagaggttct
    12541 tcgggtttct cttttcccat cccttttcca ctctttcttt ccctcctttt ttatcaccac
    12601 ttcccgggta aaatggagat tttcttcccg gatttgagct ctcagataag aaaattcttc
    12661 cccccacctt atcatttcct catcttttgc ccggcgagga gatgttcccc gggccagaag
    12721 aaacaaagcc tccagaaaat tggttttgcc ttgagcattg tctcctgcta aaaccagagt
    12781 tttctccgga aaagatagct ccactttttc caaatttctc cagttaataa actccacttc
    12841 ctctaaatac atcattactc cttgatttct accggcatca aaatatactg cagatgctca
    12901 tcttctcctc tcaacctcgt cggagcaagt tccccgctta tttctaacat tgccttttct
    12961 ccactgaaag accgtaaagt atctaacaga tatcgagcat taaaaaatat ttttatttct
    13021 tccccttcta tctctgcttc tacctcttct ttggctatcc ccacctctcc agaagaggaa
    13081 agctctattt tcccttctcc aagcaggagt tctaccactg gactctcagg aggagcaacc
    13141 aatgacactc tctccaaagc ttttctaagt tcatccccag gaagagatgt ctgagtaaca
    13201 aagctctggg gaataactct tttataatca ggatattctc cttcaataag acgagaaaaa
    13261 agagagatat cttcccactg aaaaagaata aatccttttg caggaaaaat cctcgtctct
    13321 ccctcggcga ggcgaataat ttccgtgatg gctctcagag gaaggatata ttgagctact
    13381 tccccatttt gtacttctat actgcataaa ctcaaaacgt ggtcatcact tgctaccagt
    13441 ctcaacttcc cttcttcctc aaaatgaaac aacacccctg atagagggag actgctctcg
    13501 tcttttgagg cagtgaaata aactttcatc aacccctctt tgagtttctc tccaggaaca
    13561 gaaaaagatg taattgaaga agggagagga aaaagaggaa aatcatcagc aggaaaacca
    13621 gatagtttat aatagctttt tcctcccctg atttcagagt taccatcagg taaagagaca
    13681 atttctaatt ccgggctgct caagctcttt accagattat ataacacctt acccggtaaa
    13741 accacactcc cctcttctct aacttccacc ggacagcggg aggtaatacc cacctctaaa
    13801 tcagtagcag ttagcttcaa agaattattt tccacctcta aatgtattcc cgataaggca
    13861 ggataaggcg gacgagaagc tactccccga tatactttac ccagtacctt tctgaattct
    13921 ttacttttgg ctactatatc catattcttt ctccccagtt tctcctcact actactatta
    13981 ttatttttta aatataaaaa cttagtagta gtagtaatag gataaaatta ttttgttaaa
    14041 aagtaattgg tactcattat ctacctatct tccctctcct caataacctg gttgtaaatc
    14101 cggtataagt ggttagtatt ctgtggttaa cttttctctc tttttatttt ttctattaat
    14161 tcactgattt cctggaaaag ctgtggatta ctttctatat cacccttgat tttttcgcag
    14221 gcataaagaa cggtggtgtg atctcttcct ccaaattcct caccaatgga aggaagagaa
    14281 taattggtta gctctcgtgc taagtacatg gcgatttgcc gaggataagc cacctcttta
    14341 gttctcttct tcgctttcat aagggcaggt ttaatattaa aatgttctgc caccactttt
    14401 tgaatcaatg aaatgggaat tgatgaagtt tcctgtctag gtaggaggtc ttttaatact
    14461 tcttgagcca cttccaaggt aggagttatt ttgttcaaac tgcaaaaagc cttaatcctt
    14521 atcagagctc cttctaattc tctaatatta gaaggaatat tcatggctat gaagtaaatg
    14581 atgtcagaag ggagttcgaa tttctccaat tctgcttttt tactcaaaat ggcgatccgg
    14641 gtttctaaat caggtggttg aatatcagct atcaatcccc actcaaagcg agaacggaga
    14701 cgatcttcta aagtggggat ttctttgggc ggtctatcac tggttatgac gatttgtttg
    14761 aatgcatcat gaagggtgtt aaaagtatgg aaaaactctt cctgggttct ttcttttccg
    14821 gctaaaaact ggatatcatc gatgagaagg atatctacgt ggcgatactt ctgacgaaaa
    14881 gcctcggtct ggtcgtctct aatggaatta attaattcgt tggtgaactt ctctgaagag
    14941 gcataaacta ttcgggcctg ggggttgttg ctcaaaatat ggtggcctat ggcatgcatg
    15001 aggtgagttt tgcctaaccc tactcctccg taaataaaca gaggattgta agaatgagca
    15061 gggttttccg ccacagccat agcggcagca tgagcaaatt tattactatt tcccactata
    15121 aaagtggaaa aagtatagcg agggttgaga ctgagcctat cttcccctgc aacagaagag
    15181 gaaaaactgg agacttcttc tcctaaagag gtagaggaag gctttaccac cagcccgatt
    15241 tctaaattgg gaaaattcac tcctgccact ttttgaaaag catagcgaat ggtttccaca
    15301 tatttctttt ctatcttttc tttggatagc tcacttggca cggctatggt gagccgatga
    15361 tcgagtagag atagaggttg aatgttttct acccaggctc gatattccgg tacagaaacc
    15421 tgtttttcta aaagagatag agtttttatc cacaaatcct ctaactcttc tggagagact
    15481 ttcatggctg aatctcccat ttttattttt ttaatccccc ggttttccac agatagcttt
    15541 atttactaaa ttgaatctta atcccataaa taaaaggaaa aaaaattttc ctccttattt
    15601 tgcctcgcaa aagaggggga ccgtttttgc tttttaattt aagcataaat atgaatttct
    15661 ggcaagttat tcacagctta tcaacttttg tgaataactc ggaaaacaag gaaaattcat
    15721 ttcccttttt agaagaatta ggttagtatt aaaaaattag ataattctct aaagtttttt
    15781 ctcaaaatgc cgatttttac tattgaagga aggttctttc tttttagacg aaccgggaaa
    15841 aatttgagag ggagggaaaa ccttgcaaat caaaacaaag atttctttag gttttatcgt
    15901 tcttactctg gcggccctgg ccattggagt tatagctatc tatgctttct gggaaattaa
    15961 tacttctctc gaggaagctc agagctacaa tcctctactt ttggttactt ctcgattgaa
    16021 ggatttgatt tctcaaaata actctttaat ctcctcttac tttcgagaag aagacgtaga
    16081 aaacctcaat caaatagaga aaggctttac cgaagtaaac aaccgctttc tggtttatct
    16141 cgaagcactt cgcctgggga ctgagtcaga agattttcag aaatggcact atgggttgtg
    16201 ggagaaagaa aatttcccct atgctctttc ctctctgccc gaagatagcc ctctttttca
    16261 ggaagtacag gagttaaaga gtttgcaggt caactatcag gcccgggtga atatggtgaa
    16321 aactctgtgg aagcagtatc tcaccaccca aaaggagagg aatcaaaaat ctttagagat
    16381 ggacgaacct taccagatag tagtgcagtt cactcaattg gtggatgact caataaaaca
    16441 gctaatgaac cccttagata gcacttatta ccttcttttc cgctatgcgg ttaacggtga
    16501 ccccgatggg cgcctgggag aaaacattaa tgcctacttt gccagtctga gaagagggat
    16561 agaggattct caggttctct cggaggaaag caaaaaaagc ataaccgccc aggtagaaga
    16621 tttagcgggc aagtgggaag aattagagaa agtcttgttt actgaagcag agaaagacga
    16681 caagtttatg gaagtttatc gctcctattc tcgcttgaaa actaccatag aaggtttgag
    16741 attagataac tggataaaat tgatgagtga tataaaccag aatcagaaaa actaccttct
    16801 ggcttctgac ccggtagaaa aagaagcaat aaagaaaaag gtaaacggtc ttatggatac
    16861 tttgggtcag ttcttagagg gagattttct gaaaatctat gctactaata ctgcgcaggt
    16921 ggtagtcaat gatcggtgga aacctttccg gaacctgtgg caggaagtgg taaaaggaga
    16981 tgaacagctg cgagggctca atgagaccat tcaaacctct ctttctgatg tgtgggatgt
    17041 gggagaagac ctctctcaag ctatggaaac catgaatcag gaggtgttga gctctttcga
    17101 tcaggccctt cttcgagcga gagagctaca aaaccgcctc tctcaaattc tctatatagt
    17161 tataggagtg gtgatagttt tctctctcat tatgggtacc accctttccc ggagcatcac
    17221 gagacccatt caagagagcg tggcttttgc cgaggtgctg gcagaaggag acctgactca
    17281 ggaaataacg aagaaaagaa aagacgagat gggtaaactc tttgcttctc tgaaccaggc
    17341 ttcttcttct cttcgccgat tcctccaaga ggtaagagag ggagcggata agattatttc
    17401 ctctctggat gccctccagc acacctctcg agaaataaga gaggcgggag agcagattgc
    17461 ccaaactgtc tcgcaagtag cccgtggatc ggaagaacag agccagaact taactgaagt
    17521 ttctaaccac atggagagcc tggtggaaga agtcaaagag atggcgaaga ggctgaaaga
    17581 acaggctgat aaagctcgcc agactttaca agagaccgat aaagtgatag agagcttaga
    17641 ggtaaccgga ggaaacatag aaagagtaaa aggagcagct tcttcggctt tccaggctac
    17701 cgaagaagga gagaagacct tagaagaagt agtggaagcc atgggaagaa ttaaagattc
    17761 agtgttttca gtgggagaag tagtgaaaaa cttaggagta agttctcagg agattggaag
    17821 catcaccgac ctcataaccg gtattgccga ggagaccaac ctcttagctc tgaatgcagc
    17881 aattgaagct gctcgagcag gagatgcagg acgagggttt gcagtggtcg cagatgaagt
    17941 gagaaagctg gctgaagaat cggcccatgc tgcccagcgc attgctcatc tcatcagtga
    18001 aatccagaaa gaagcggaaa gagcagtaag atctatgacc gaaagccaga cccatgtaga
    18061 aggaggaagt gaggcagtgg ataaagcccg agaggccttc caggatattc acgaggctaa
    18121 tcaggtggta acccaggagg ccgacaacat ctctctctcc ttccaggtag tagaagaagc
    18181 ctcgcagcac atcattcagc tggttcggga agttaatgat attgctaaag agagtgaaga
    18241 aggaacaggt aggatagtag aaagagcgga agaggtattt acaaggctga gtagtgttgc
    18301 ttctatttcg gaggagaacg cagcagccgc tgaggaagta gccgcttctt cccaggagca
    18361 aaatgctgcc ttgcaagaag tagatgttac catccaggaa ctgggtaaga tggccgaagg
    18421 cctgaaagaa aacttaggac agttcaaaat ctaataaaaa aatagccggg gggagacaca
    18481 tctcccccct atttataccc cctttttgat ttcctcttga gaaaatactc ttttagttat
    18541 agaattaata taatacggag agggacttcc tgaggatgag aggggaaaag aaactaccta
    18601 ttcttttaat tttaatcttc ctcctctttt tctctttccc tggagaggca aaaagagcgc
    18661 ttttagtggg ggtttgcgac aaccctcctc tggttttccg ggatgagaaa gggagggtgc
    18721 ggggaatgtg cccggaggtt ttagagcata ttgctcagag agaagactgg gatatcgaat
    18781 atgtttttct ccccttttct cttcttcttt ctcatcttgc agagggaaag attgacctcc
    18841 ttttacccat tgcctacacc gcagagcggg cagaacactt tgatttcaac cagcaaacct
    18901 ttttggttaa ctggggagaa gtttataccc ttcctgctgt ttccgttcag tctttgctcg
    18961 atttagcttc tcgaaaagta gcagtggtaa aagacgacgt ttatggtgag agtttaaaaa
    19021 aactccttcg agacttcggg gtcgagtgct attttctgga aatggaaaat tatccgggag
    19081 tgtttgccgc agtaaaagag ggaagagctg atgccggagt ggtgagcagg ctctatggag
    19141 cgcaatttgc tagagcggct ggtttggcag ccacctccat cgttctttct cctgtggaac
    19201 tccgttttgc cgtccctcga ggaaaaaacc gaggaatcat cgacattatc gaccagcatc
    19261 ttctggcttt gaagatcaac ccccaatctg tttactatca gtctttgagc agctggttaa
    19321 gtgtagaaga ggagggatgg cgttttcctt cctggttctg gtggttttta ggcgttgttt
    19381 tgctctttct atgcctttct ctctttttaa gttattttct gcgccgggaa gtggggcgcc
    19441 ggactaaaga gctggtagaa aaaagcgaaa aattagctga agagatagaa agacgggagg
    19501 ctttagaaga agagctccgc ttcggcctcg acttccagaa gctcgttgcc ggggtttcct
    19561 ttcattttgt cactgcttct tcctttgaag aagcagtgag atttttctta gaggaggtaa
    19621 gacgtttttc ccgggcagat gaagcggctt tttttgggga agaagaaggc taccttggga
    19681 gagaggggaa cacagaaaaa gtagagatag aagagggaat ggttctctgg agagaaagat
    19741 tgtgtttgga aggaccggtg tggacagaag gggaaagcct tcccgagaga tgctggagga
    19801 agaaagagcc tggagaaatt agttttctcc tctctttccc tctcgagatc gaaaaccggt
    19861 gtctggggtt ttgggaatta ctctggagag aaaaaggaaa cacagataaa acatggatag
    19921 aagaacatct tgaccttttc caggtttttt cctacctttt gagtaacgcc ttagaaaggg
    19981 agaaaacaag aaaggacctg cagcgagaaa gaggttggct ggagaccatt ctccgctcca
    20041 ttgctgatgg tgtgatagtt actgatttgg agggactggt cagttttatg aatcgagagg
    20101 cggaagccct tactggttgg tctttaggag aagcccgggg taagaaactg gaagagattt
    20161 ttcctctcag agaaaaaaat agcggaaagc cggtagagaa cctggtggag gaagttttga
    20221 aaaaaggaaa aacagaaatg aaagaacctc ttcttttgag aaaccgagaa gggaaggaaa
    20281 tagtggttgc cgatagtgga gcttctgttg gtagagaggg agagctccaa ggagtggtgt
    20341 ttgtattccg ggatgttact gaacgccaga ggatggaaga ggcgctccgc aggagtgagc
    20401 gccgttatcg cttgcttttt aaccagatgc tggatggatt tgctcttctg gaagagattt
    20461 atgatgggag aggagagcct tcttacgagt ttgtgttttt agaagccaac cctgctttcg
    20521 agcacattac cggttttagc agagaagatg tggtggggct tactctccgg gaggtcttcc
    20581 cctttttaga tgattcttac tgggagcttt ttcatcaggt ggcagagaaa gaagaagctg
    20641 cccgggtaga aaggtttgtt tcctataagg ataaatactt agaaatagtg gctttctctc
    20701 cttcccccgg acaggtggct actatctggc gggacgttac agaaaaaaga aaaagagagg
    20761 aagaaatgcg ctatcttagc ttccacgatg ttttgaccgg actttataac cgggcttttt
    20821 ttgaagagga aatacagcgt ctggatagag gaaggcatct tcctttgagc ctcatttttc
    20881 ttgatgttga tggcttaaag gaggtaaacg attctctggg gcacgaagaa ggagataggc
    20941 tgttaggaga aatagctcaa gtgcttcgga aatccacccg gggaggagat gtcatcaccc
    21001 gatggggagg agatgaattc attgtccttc ttcctcaaac cactgaagac attgctcgag
    21061 aggtgggcaa gaggatagag aaaaactgtg aggaagcaca aagagaatgc cttctaccct
    21121 taagcctttc ctggggagtg gccaccaaag agagcgacac agaagacata agagaaattt
    21181 tccgcagagc agaagaagag gcttatcaga aaaaattctt aacccgcaag gtttttctcc
    21241 gctcccttat ttctattttg gtacacagat tttatgagga agaaaaaatt cggcagcgtc
    21301 agatgagggt gggagaattg atccagaaga tgggagaaaa acggggatgg gaagaagaag
    21361 agaagaaaaa gctgcacttt ttgaacctcc tctgggtctg ggaaaagatg tttctccccg
    21421 agcacctcaa agaggatggc tatccgcttt tgcgagagga attggttgaa atctaccctg
    21481 agtttgaaga gcagcttccc ctttcatact cagggtggag ctctttgttt tcaagggttt
    21541 tatccggaga ggaatactac ccgggggaag gagatgaagt gcctctcatg gctcgccttt
    21601 tgagcgtagt agaggcttat gagtttctgg tggaggagcg acagctaact cctcaggaag
    21661 cggtgcggga aatccagaaa gaagcaggaa gtcgctttga tccggaattg gtagcaatat
    21721 tagaagagat agtaagagaa gaataattta tgtgccggga tattttccgg cacttattgt
    21781 aaggacgaaa aaagcgagag ctccgtggtt ctggccaagt ttcgcagcaa tctgggtgaa
    21841 ggggagctct ttgcaaaacc gggacttcac ccgattgacc tctggggtgg ttgagggtat
    21901 aatgataccc gtggtaaatt acccattttg aagcagcaaa gaagggagag acgttgatga
    21961 aaaggactta tcagccccat cgagtaccga tgaaaaggac tcatggattt cgggctcgga
    22021 tgaagaccaa agggggaaga caggttctct ctagaagaag gaaaaagggg cgcaagcgtc
    22081 ttactgttta ataaatggag ggattgagcc agaagaaaga tttttcccgg ttgttttctc
    22141 ggggaaaaag agcgagagcc aacaatatta agctgtggta tttgcaaaaa gaagagggga
    22201 atttgcgagt tacttttgtg ggaaaaggga aaaaggcggt agacagaaac cgagtgcgaa
    22261 ggcgcctgcg agaagccttt cgtgtctatt attatcctca gtggagagac aagccggttg
    22321 attttctctt tatgagcaat gagaagctgc tggaagagag ctttgaagac cttgttgaga
    22381 tgatgggaaa ggtgctggga gaagtgaagc ttgatggagt aaagacgaaa agtgaagctg
    22441 agtagcctcg tagtaagcgg tttagataaa attttgcttt tttatcagag atatatttct
    22501 cctctttttc ccccctgctg ccgttttgaa ccttcttgtt cagaatatgc ccggcaggct
    22561 ctttgtaaat atggtccttg gaagggtggt cttttggctc ttcgaaggct tttgcgctgt
    22621 cacccctgct ctccaggcgg ttatgacccg gtaaaataaa aagaaaaggt ggttaattga
    22681 tgttttcaca gctattagat gtgctagctc aagctatgga atacctgctc cgttttttct
    22741 acaactggac cggcaattat ggtatttcca taattctcct cactttattt attagactga
    22801 ttctctctcc tttgattcat aaacagaatc tctctacccg caagatgcaa gaagacatgc
    22861 aggagttgca gccggaaatc aaaaaactgc aggagaaata tggcaataac agccagaaac
    22921 tgaacgaaga aatgatgaag ctttacaaag aaaagggaat aaatcctttg ggagggtgct
    22981 tgcccgggtg tcttcctctt ttaattcagt tacccatatt gatggtgctt taccgagttt
    23041 taatggctta tgactatggg caagctgggt ttctctggct ccccagcctc tctcaaaaag
    23101 acccttactt tatccttcct cttttaatgg gagtaactac tttctggcag caaagaatat
    23161 ccatgcctcc cgccgcagaa ggagcacaac aacagaacgc ggccatgatg gtggttatgc
    23221 ccatattttt ggtttttatc agctggaacc tcccctccgg agtgcttttg tactggtttg
    23281 tgtctaacct gttttatatt ttgcaacagt atgtattgaa caaccagatc cagaggtcta
    23341 aatctctctc cattgagcca gaaatcagtg tgaaggccga gcctgctgtt tctctaaaag
    23401 acccctcttc agcagcacct gtagctaaaa aaggaggaaa aataaatgcc aagaagcgtt
    23461 gaggtttcgg gaaaaagctt agaagaagca ctggagcgag cggctcgcta ttttgatgtg
    23521 ctgccagaaa gcttaagtta tgaggttatt tcggaaggcc gtggtttttt gagtgccttt
    23581 accccccggg tggttaaggt tcgggtgtgg atagaagaaa ctccccagga gagaattcct
    23641 ggtgaagaaa aatccccggg aaaagaagct acagaggaag cttatccttc cccggcttct
    23701 cttcttcggg cagaggaaga actggggaaa ttttttaaag agctaatgga caaaatgcag
    23761 atggaggtcg atttccaggt tcaagagaaa gacggcttcc tttctctctt cgtggaagga
    23821 aaagatgccg ggatgcttat tggtaaaaga ggagaaactc tggaagcctt agaaaccctg
    23881 ttgcggatag tcatgtccaa aaaaggcttt agtaatgtag gcttgcagct ggacatttct
    23941 ggctatagag aaaggcggga agaagcgctg aggaggttgg cggagaaatc ggccaaaaag
    24001 gtaataaaag aaaaaagacg gctgaagctg gagcctatga atgcccggga acggagaatt
    24061 attcacactt ctcttaaaga ctatccagag gtgaccacct acagcgttgg ggaaggaacg
    24121 gggagacggg tggttgttga cttaaaaaag gaaaaaagca gtaatcgtag tggaggaaat
    24181 aaaagacctg ttggtaagtg atactattgt agctccggcc acccctctcg gggtgggagc
    24241 gattggcatt gtgcgaatga gcggtcggga agcggctgaa attgcttctc ggctttttgt
    24301 ttctcgctgt gggcgtgcgg tggaagactt tgtcagtcat cgcctgtatc tgggtgatgt
    24361 ttttgaccct caaactcaag aaatagttga cgaagccctt cttgtggtga tgagggctcc
    24421 tcacagctat acccgggaag acgtggtaga atttcaactg cacgggggtc cggtggtgat
    24481 tcaaaaagtt ctgcagctta tccttcgaga aggagcgagg ctggccgaac caggagaatt
    24541 tactcgccga gcctttctaa atggtcgtat agacctttct caggccgagg cggtggcggg
    24601 aatagttaag gctcgctcag aaaaggctct ttctctttct cttcgcaatt tgaaggggga
    24661 atggggaaga aaaatccgct cctgggaaga tcgtcttttg agcatcctgg ctctcattca
    24721 cgctcactgc gattttttag atgaagagga gcttcctctt gaagccatgg agaaagagct
    24781 cgcttcgtta atagaagaaa tagagaaaga gctgtcttct ggtgagcgcc ttaagctggc
    24841 gcaagagggg gcgctcgtgg ctattgtggg taaacccaat gtgggcaaat ccagcctttt
    24901 taatgccatt gtgggatggg atagggccat tgtgactcct tttcccggaa ccacccggga
    24961 tgccctggaa gaaaatattc tcgtggatgg tcttcttctt cggctggtgg atacagcagg
    25021 gatgagggag gggcgcgacc cagtagagct tgtgggagtg caaaaagcag aagagtatct
    25081 tgaacaggca gactttgtag tggtagtttt agaccagagc atccctttag aaaaagaaga
    25141 tgaagtgctg gcggagaaag taagaaataa gccccatttt gtggtgctaa acaaaagcga
    25201 ccttcttcct cagctaaaca aaagcgacgt ttccttgctt tatcccgggg aggagatttt
    25261 ggaagtgtct gctctgcagg gagaggggat agaaaagcta ctgagaaaaa taggggaaag
    25321 aataaaggcg gaaataggtc ctgaagacca gaccatttta atcaccgccc ggcaaagaga
    25381 ggggctgaaa aaagcccttc tctcattagt ccaggcccga gatagcttaa gagaggggat
    25441 gtctttagat gttctgggtt tagacctgga agaggctttg cgagaaatgg gacggatatt
    25501 aggcacacaa gcagataaag agttgttgaa tactattttt tctcaatttt gcctgggaaa
    25561 ataatttgtt ccacgtggaa cacgaacctg cccagaaaga aggacggcct tttgccacgc
    25621 tatatagcta taattctgct ttgttgcaag ggtcattgcg ccacggaccg agggtgaatc
    25681 aactataaag gaacactgct ccttcataac ccccgaaaaa atcaaaagaa aagcaaaaac
    25741 ttggattccc gactactgat ttcgggaatg acggatgatg tggatgcctc gctaaatttc
    25801 aggcatgacg atagaggcgt cacttctgaa tggttccacc gtcccttttt cgtcaccccg
    25861 gcgactttcg agccgttttt agcctgcccc tgaatgcttc tat
//
LOCUS       DAOPSS010000007        23731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_1763_length_23731_cov_9.055795, whole genome shotgun sequence.
ACCESSION   DAOPSS010000007 DAOPSS010000000
VERSION     DAOPSS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 23731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23731
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1763_length_23731_cov_9.055795"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(625..1191)
                     /locus_tag="PKN31_01050"
     CDS             complement(625..1191)
                     /locus_tag="PKN31_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOA98547.1"
                     /translation="MKIVAIMGSPRRGGNSDIMLDAFLSPRDSLHSLEWIVPSRLNLG
                     FCRGCRFCEAMGRCILRDDMDEVGEKLLEADKVVLSSPVFFYGFPASLKALVDRAQVF
                     WSRKYKMGEKFKPKEGFLLAVGATSGGKLFEGIVLTTRYFFDAWGCEYRGGLFFRGFD
                     QKGAIGECQECLEEIKEAGRSFLSSKNS"
     gene            complement(1224..1748)
                     /locus_tag="PKN31_01055"
     CDS             complement(1224..1748)
                     /locus_tag="PKN31_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOA98548.1"
                     /translation="MTIQEVLEQALKMEEDGRQFYLEGYERVSSPLSKEVLKRLADEE
                     LVHIERIKEGYNKVTKGEEFAVENWGGIRKPAREYFDNIFTEARKNMDELITPSAQEL
                     DVIQTSMDLESKSHQFYVEKSKEVEDGKVREFLDFLASEEYRHYNLLFNTYEYLSNPT
                     SWYYREEKPIFEGG"
     gene            complement(1745..2137)
                     /locus_tag="PKN31_01060"
     CDS             complement(1745..2137)
                     /locus_tag="PKN31_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015591595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOA98549.1"
                     /translation="MEEKIFVIQEHWASRHHFDFRLEKDGVLKSWAIPRSIPQNAGEK
                     RLAIEVEDHDLDYADFEGEITEGYGKGKVKIWDRGYYLADKWEEDEILLELKGNKVKG
                     KYALIRTKGSKADDNRQWLLIKRKEDVS"
     gene            2701..3918
                     /locus_tag="PKN31_01065"
     CDS             2701..3918
                     /locus_tag="PKN31_01065"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOA98550.1"
                     /translation="MPEVLENLKSLLKKAESKMVLLVADGLGGVPHPDFGYQTELEAA
                     RTPNLDELAKKSALGLMTLVNHGITPGSGPGHIGLFGYNPEELTIGRGVLEALGVGFH
                     LQPGDIAARANFATRDERGIIVDRRAGRISTEECTRLVEKISREIKEIEGVEIILRPG
                     MEHRFVLILRGEGLKEGVRDTDPQKEGLPPYEPQALDAEAEKTARVLKLFLEKAHQII
                     KDEPKANTILMRGFSQSPHIEKFPERYGVRALAIAVYPMYKGISRLFGFDTPDVTGDF
                     QDEVKLLRDKWFDYDFFFLHYKDTDKAGEDGDFKKKVDRIEHLDQFIPQVLALRPDVL
                     AITGDHSTPSLLHSHSWHPSPVLLYSKYAGVSTGTRFTEKECAHGYLGQFPSRALMSL
                     MLANALRLGKFGA"
     gene            complement(3944..5023)
                     /gene="hydE"
                     /locus_tag="PKN31_01070"
     CDS             complement(3944..5023)
                     /gene="hydE"
                     /locus_tag="PKN31_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOA98551.1"
                     /translation="MRNSGKEWDLLNKLDREENLTQEEIVILLGCRDRKVEEQLFYLA
                     DEKRKKYVGEEVHLRGLVEFSNFCRRNCLYCGLRRDNRKLKRYRMSLEELYNLAEKIA
                     LQGLKTIVLQSGEDLAYDAKSLASLIHRIKKLDVAVALSIGEREPWEYALWRDAGADR
                     YLLKQETFDRELFAKLHPDDDFDQRLECQRTLKELGYEVGSGNVVGLPYQTLQSLALD
                     IKKFQEWDFDMIGIGPFIPNPDTPLGEEVSNPEGKYLLTLKVLALTRVLTKTSNLPAT
                     TALGTLIPGGREKALSCGANVFMPNLTPSFYQSRYIIYPGKGIEESWENYLLEVKKMI
                     ERKGRVVSTGYGNRVSSGSGEAGRR"
     gene            complement(5446..6450)
                     /locus_tag="PKN31_01075"
     CDS             complement(5446..6450)
                     /locus_tag="PKN31_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA98552.1"
                     /translation="MRKNADSLYDLALRFYEKGKRAIVPLFGYSGLQLTGTTIKQNQF
                     NHLVQFRSLSRLYDRFRPDAMFFLMDLSVEASALGLPVRFPLEETPSVEAHLVKSKDD
                     LDKFRKIDILGDGRVIVYLETLKHMRAAFPCPVGAYVIGPLTLSGLLMGANQLAIKSI
                     LEPDFFEEVLEFSHQVVMRYAQALRENGADSIMALDPTAVIFGPQQFRQTISRFYREM
                     VGILDDVEITLHVCGNTFHLLEEMVDSGVTGLSLDSLVDMREASQVTGEDILLMGNVS
                     PVKMLFGSPQEVYDETWDLLESMRDFPPFILSTGCDLPQDVPLENIDAFFQAGRNWGK
                     "
     gene            complement(6786..7631)
                     /locus_tag="PKN31_01080"
     CDS             complement(6786..7631)
                     /locus_tag="PKN31_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOA98553.1"
                     /translation="MRKKRRWTNIILWIAVIVAVVWMLFPFYWAVITSIKKPADVFRL
                     SFIPFVQFEPTSENWRSELQMRGQEIKRGLKNSLIIGVCSALVALFIGALAGYGLARY
                     KYHRVKNRDITMWILSQRFLPPAAVVIPFFLIFKTLGLIDSVVGLVLINATFGIPFAV
                     LVTRDAFRDLPEEIEESALVDGCSRFQVFSRIALPLAAPALVAAGIICFAFAWNEFLF
                     AFIMSYSKAVPMTVLIAGTQDTQGIQFWYVATRMLMAVVPPTILALTVQRYIVQGLTF
                     GAVKG"
     gene            complement(7647..8525)
                     /locus_tag="PKN31_01085"
     CDS             complement(7647..8525)
                     /locus_tag="PKN31_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOA98554.1"
                     /translation="MNRKKRVPLKEQGKHFFIFPAIIWLLVFTIFPLLYTLYLSFHGS
                     RFLRVTKFVGLENYIYVLSDYRFWNAFRVTITFVVLSVLLTVGLGLLLALLFNRQMKG
                     VQFFRTICTTPLFTAPVALGYMFMIILYEEGGPVNNFLHALGANAIPWLSHPRWALVS
                     VLIADVWQWTPFAFLVLLAALQAAPRDLYEAASLDSASGWEIFRHITLPLISPTLGTV
                     IILRTVEAFKVFDLPFALTNGGPGLATRTLTFNVYTSGLRDQNLGVATATAVILLLMV
                     LAVSQIFFRRYGSNYD"
     gene            complement(8585..10213)
                     /locus_tag="PKN31_01090"
     CDS             complement(8585..10213)
                     /locus_tag="PKN31_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOA98555.1"
                     /translation="MSKFFRKSVFWGLVILLLFSLSLVAFAQEEMSELEKKTADEAFA
                     KADKGQPDPDWKGKKFTIAVLASGPHGAISGPLYFWRPYWEELTGATYDIAEIPFGEL
                     QAKIFTDFATGTGNYDCIVGPSFFYGDYIANDWIIPIDKYFDDPRMPEWDRESIAPPI
                     RELLQWGDNWYGFNNDHDGMVIYYRKDLFNDPRWQAEFKEEYGYDFPVSPVTWQEILD
                     IAKFFNGKDWNGDGKDDFGITMHLKVGEQGFFNYIALAGSFVCMPAPGDDPAKITRYH
                     NVFWFDPETMEPLINTPGHVKALEMLTELTKAGPSAMVGWGLGEAWDVFLRGNAAMCF
                     TFGDVGTLSQDPRVSVVQGKLGVAPIPGSKEVYDLESEQWVTLEEPNLVGNKCGASWH
                     GVISSYAEDPDMIAHFLSWQATPEINHWNVAWGWTGIDPGTTYDFLEPAGKAKIEDYT
                     ITGYDAEDITQFLDAYYTLWFEYPRSVPYLRIAGAADYIESLDIHMSEALTGQATPQE
                     AWNRAAQDWNRITDQLGREVQKELYKEAIGYSGE"
     gene            complement(10394..10792)
                     /locus_tag="PKN31_01095"
     CDS             complement(10394..10792)
                     /locus_tag="PKN31_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOA98556.1"
                     /translation="MPNEFTVRTHSQEEFIDITNSVKEVVTSSHIQKGVAVVYVPHTT
                     AGVTINESADPSVVEDILEVLRRVVPFNFPYRHLEGNASAHIKTSIIGSSISVLVERG
                     NLILGTWQGIFFCEFDGPRTRRVWVDVIGE"
     gene            complement(10804..12738)
                     /locus_tag="PKN31_01100"
     CDS             complement(10804..12738)
                     /locus_tag="PKN31_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA98557.1"
                     /translation="MSEFIGTVTGLEEINPYCFFVRVNSSYSSTVALDQVVRVDYRWG
                     DREFHAYGMITEVKAKWDGTISTGYQEEAYNEGIYQGVPIYLGKVVVTRVLEKKEEDL
                     VPVPYSFPPPAGEKVFVACGEELEVALGFSEIRKGKRALPCGILPSGDVAYLDLKYIL
                     GDNGAHINISGQSGVAAKTSYATFLLWMLMNYSRYVKDNAFSNQLVNSRSLVFNVKGE
                     SLLFLDYWNEEWKRAQKKDSEEGRQFAEWQEMFRLFEVEPHPFSELEVYVPPRNEKGS
                     PHIRHSRPEVKAYGWDILDIVELGLLPLMFDPEELSQNPNFLLTVTVIEDFLRDQYDG
                     LVADARRELRSGSYPVKIDLQGLTDQEIVRVYLQAAGEEPRLILESFSLPNNLISLIT
                     MLRLSLEGEGKLGEELQREQIDPRTTLAISRRLRAARRTGLNLLWRDITRLPYREVKE
                     VPEGGYHLRWNRPGGISVVDISKLTYQGQAFVVGAVLREIMHWKERERLAEPVFVYLD
                     ELNKYAPRTGGGPLGNIFRDVAERGRSFRVVLIGAEQTASQVDYRVITQAATTVVGRQ
                     KLAELHKEEYAHLEGNLRDKAATLLPGEVIIDQPFLRIPLTVKFPLPPWATSEENREE
                     LYRKEVVFTPEEELKKLLGE"
     gene            complement(12731..13804)
                     /locus_tag="PKN31_01105"
     CDS             complement(12731..13804)
                     /locus_tag="PKN31_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98558.1"
                     /translation="MEEKRGSYLTLTDFLREKMAQVDHWFYEEDSFPRFFREENDGSL
                     AASELWEIEEGDFPSWSGKRPSPGLEEIYLVDGRMRIHARIIVLMKTALLGEVAAGFA
                     WWQKDRGLSLGFSPQNPPKLERVLGAPEGLFSGEMVSRELSLGPGFVFHVWESARASR
                     YPQELEAAQQAIFSAMQVLESKVVKDLLSRSSCIIKDGTLHEGDPEFQPGVGPCGLVK
                     RVEDLHLPHSYLESLFKLERGERTPFFSAYLKKDSPTLRVFCYTRLVERDDNYPWKGL
                     VRLETLLNKERWTEEKDNLSLFFDGVVSLLPFLTADFPAKRLPENIFPVIALEEHLGQ
                     YFSSPPFVQGLIRKTLGRKEKDE"
     gene            complement(13777..16911)
                     /locus_tag="PKN31_01110"
     CDS             complement(13777..16911)
                     /locus_tag="PKN31_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOA98559.1"
                     /translation="MRPLELEVEKFLGLESLSLDFDSSFFVLVGPNGAGKSSILEAIF
                     FALFGRGIRLERGKRELIHRGYPEGPLRVKLKFLLGGNEFQVVREYSPRGGVAVLEKK
                     ENGVWRGLASGEQLVNKKIEEIMGFDYLTFKSSVFLPQGETLSFVEATPAERFKTLSS
                     LFGLELLDVIRERVKEELRSLERELLPEESKLEELEKENLEEQLNLAKEEKKDLEDKT
                     ADLAGEEKDKKRRTELLNRLTEIKNNEKELQKKHDDLEKERDRAYKKAQEDREIQKAW
                     EVKASFWQPWQIVVERVEKWEKDKKAWLGKKAKIEEEIAQLSKNLQEIGEKIKEQETK
                     KNNLASYEDKLRREALPLAEEARAIKRELQELEKDKERIRKKIEELEIILQKEEKEEK
                     ELERKITEEKEKIKKEEEIIQKVEKAEEKIEPLLKELGEKEKEISLIAVELKSLQKDI
                     ELSSERYRKLEREWEKKRKKLSLWQEEKKAREEDYRQMVKKFALWELEKEWEEKGFCP
                     LCGSNVPPPSEAENLPEVDWEGYHSFQEQGEKLVEEESSLRQEKTQEESKIAELKNKE
                     EEKLEKKKKLEEEKNRILEVLKNIDLPPLVKPESIEGFLKEKRGEREKALFNLYQLEK
                     NREVKKAKIESRIKEKDEERVKIESIEKNIKEEEKFLTEKREQLSQIFAVLRWEDDRF
                     PEEAFSDLWDKFSKRLQTLSRELEELGKEESRSEEKLKLLWQNKEEAEEKVAELLQQG
                     EELQKEEEKKRKVFQEELSRLGWSAEYFEQLRDKKAGNWQEIWHRLGGEIKQTENSLQ
                     DCQEKKISLIQQLEIKEEEAEEQLMQEKEILEELQKELGKIREKKGSLDKEIENLQAK
                     IEERDRLREGLQEKRKNWELHYQLDKALEARGFRSYLLGFLFRELEEEASSFLRDLSQ
                     GRYFLRMKMEEGKAEMVVVDNNYGGEERLPSECSGGEKTLVALSLALALSRIHLREVG
                     RERGADCLFIDEGFSALDQEHLELVADAILRLSQDGRMVGIVTHDPSFAHYFPLHLEV
                     RGGKAFWKRNEEVI"
     gene            complement(16898..18052)
                     /locus_tag="PKN31_01115"
     CDS             complement(16898..18052)
                     /locus_tag="PKN31_01115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOA98560.1"
                     /translation="MKKKIKILHTADWHLGLVSWKTRREVDRGEEQREALEELIGVAE
                     REKVDLVVHAGDLFHSSHHPDKKTIKLAVDTILRLVDIAPFVWVMGNHDWYAIEGLKK
                     IFPPQAYIIRDYTPREIEGIPVTVFPLPYVSLARLLGDYRGDEIQDRARELLRQEMKK
                     WERSFLPDRWNILVAHATIEELARYAEANPHREVFLKKADIPSAFNYGALGHLHTFSF
                     LRSPCPLYYPSSLVVDSFEHVGKEGSFLLVELEEGKESKVEPYFLSGAHLWDWELKEE
                     KEIEEIYEEISQDMTGKRNYIRLRIKEDFYTPALANKLKEMGGENWQIVVVEVIPREK
                     KEVEEEPLPVNEDSIPQLFSRYCQEQNLSEEVVKLFYRYYQEAGEISETS"
     gene            18113..18250
                     /locus_tag="PKN31_01120"
     CDS             18113..18250
                     /locus_tag="PKN31_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98561.1"
                     /translation="MITIYYIIEKLIIVNRKAHGNVPLGTMFSFHAVEFIVWQATKKS
                     L"
     gene            18718..19716
                     /locus_tag="PKN31_01125"
     CDS             18718..19716
                     /locus_tag="PKN31_01125"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOA98562.1"
                     /translation="MEVNVFILLGFLLGYGFRLEGDYLFALEASSPDYLLDLQPIRSD
                     RFTIYSPYPEEDFRFKGQIHCHTQASDGDDTLQEVMEAYQSLGYHFVVITDHDQITKA
                     PAVPGILFIPGVEKSSSSGHILGIGVKSQTDEMRPDKIIDHIQKEGGVAIYAHPNRWP
                     LFSGWTLRELLSVPGACGIEVINAKRWRTNSENKWDALLTRGYRIWGMAGDDCHSIAR
                     EANRAWVVVAAPDLREDSIIAALRNGHFYSTEGPDLSVKVYPDKIVASTSREATIEWI
                     AKGGVKRRVIRGVKEDTYFVEGDEGYIRVVVIRKSDKKRALGQPIFIEGAGASSKG"
     gene            complement(19769..20686)
                     /gene="radC"
                     /locus_tag="PKN31_01130"
     CDS             complement(19769..20686)
                     /gene="radC"
                     /locus_tag="PKN31_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOA98563.1"
                     /translation="MNSPGEELLDFMVANAIYPFCSKKGKIFLRCSKKGILTEGKKKE
                     IIEQLNIETGDECETLREEIKKQVAERRTTRPIKEWIKEERPREMLARLGAENLPLSK
                     LLAIILRTGKNGSSAEELGQTLLNHFGSLRAIDAAPISELSKIEGIGPAKAAQIKASL
                     ELGKRLFQERASTRKKLTRPDDVISYVSEYYAPYLRDKEKEFFNVILLDIKNKPIDNV
                     EISKGSVNATIVDPKEIVRAASLKSASSVILVHNHPSGETTPSQDDIELTNRIVSACE
                     LLGIKVLDHVIIGKNMEDFYSFAKEGLIG"
     gene            20995..22845
                     /locus_tag="PKN31_01135"
     CDS             20995..22845
                     /locus_tag="PKN31_01135"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOA98564.1"
                     /translation="MAKERANKISLKPSKRQLVFPFLSTFAKLITEMKKKQLKLIFLL
                     LVIFSLFSVSVQAQDVVAWRDFSQELTRLLQLPSGRIPDFGEGEKPITRLEAAKMVIQ
                     ALSYQDLLSFVDTKSVAFSDLSNLSSEDQKWMILACTLEPPLFQGDSTGKLRPKDPLT
                     KSEFLQLKEKISRYARGDVSWQREKVINQYITLLVKKEGFSPSPSLETTQAKGPLSGQ
                     VMLQVGAFQDQERAERTATWLKELGYTTAQVKKEGDLFKVRIGPYSKEEASQVSARLQ
                     KQGFVSYQITTDTTPTVTPTTPVGPFFSVGLLFDPNSPLRLEIALAQDKVLEKEKTSE
                     IAQRKGAIFAVNAGFFAQDGDPLGTLMIDGRILSEPQEGWYSVGITENNQLLFGELKF
                     KAQAQAQNGATYSITGINRRNNGKELVFYDHFLGEKTPKQEGIESIIVKGKVIETKSS
                     QGGTVIPTDGFILQGRGEASQWMWKNLIPGSSVKVKMSIYPKDEDWERWKDVRYILSS
                     GPLLFRNGQPGPFGNFNEKIVTEKHPRTVIAQTEEGKILFLVVDGRNPAHSVGLTIPE
                     LVEELKNYRVVNALNLDGGGSTTFYLQGQILNQPSDPLGERKVSNAFIIR"
     gene            complement(22851..23513)
                     /locus_tag="PKN31_01140"
     CDS             complement(22851..23513)
                     /locus_tag="PKN31_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOA98565.1"
                     /translation="MIKAIIFDIDGTITDTNPLFLRSISQTFREFMGVEKPENFFVFT
                     VGIPSPETLKALNIPPEMERAFVARWQELIREGMREVRLFSGVREVIEELHQEGLKMA
                     LVTSKIRSEMSYQFDHFMINHFFETIICAEDTPRPKPHPDPLLLALDRLGVKNKEAIF
                     IGDSIYDIQAAKKALIPFALASWGTLELEKVLELHPEFVLEEPEDIGEIVRGERDLTP
                     AL"
BASE COUNT         6832 a         5572 c         4447 g         6880 t
ORIGIN      
        1 ttaccagaaa atgggagtcc ttaaaaagaa aagcctgtta tagcgctaat ttctggcact
       61 tatagcgcaa gttgggttaa ggaagaagta gctgaattat cagatacatc attatcaaag
      121 gggaaaagag gaagaaaata aatcagaata tcgagactgt gcatcaggaa aggcaaaacc
      181 taaagatgaa agatgggagt atatatttta ggaagggtaa gaacggaaga accattccgc
      241 actaaactct tataaacccc cttctcgtct gtcgcctctg aatgtatcca tcggaggtcc
      301 atagtattta aaagcatttg gattcccgac tactgatttc aggaatgacg taaaaggggc
      361 cgttactcca gaatggttca gaataataag ggggaaagcc cctttataac gcctcttaaa
      421 acgataaaaa gcaaaaactg ctgaggaaaa aagcttcctc agtaaaaaca tatagatacc
      481 tgattagagg tttcaggcat gacagaaaag agcaagagga ggagaactct cggggttacc
      541 acgcactaga caccaggtat gaaagaaaaa tggcgcgcta atagagggaa ttaccatgcc
      601 ttttgcagtt aaatgtaaaa actctcaact atttttggag ctcaaaaaac ttctccccgc
      661 ttccttaatc tcttctaaac actcctggca ttctccaata gctccttttt ggtcaaaacc
      721 ccggaaaaaa agacctcctc tatattcaca tccccaggca tcgaaaaaat acctggtggt
      781 taaaactatt ccctcaaaaa gttttcctcc actagtggct cctactgcaa gcaagaaacc
      841 ctccttgggc ttaaacttct ctcccatttt atatttcctg gaccagaaaa cctgcgctct
      901 atccaccagc gctttcagag aagcaggaaa accataaaag aaaactggag aacttaagac
      961 caccttatcg gcctcgagta atttttctcc tacctcatcc atatcgtctc ttaaaatgca
     1021 tcttcccata gcctcacaaa accggcatcc tcgacaaaac cctaagttga gccgtgaggg
     1081 aactatccat tctaaagagt gaagggagtc cctcggggaa agaaaagcat ccagcattat
     1141 atcgctattc ccccctcgcc ggggactccc cataatggcc acaattttca tactttcaga
     1201 tcatcccctc ccccgaggag ttctcaacct ccctcaaaaa tgggtttttc ctctcggtaa
     1261 taccaggagg tcgggttact caagtattca taggtgttaa acagaaggtt atagtgacga
     1321 tattcctcag aagctaaaaa atctaaaaac tcccgtactt ttccatcttc tacttcttta
     1381 cttttttcca cgtagaattg atgggatttc gattccagat ccatagaggt ctggattaca
     1441 tccagctctt gagcgctggg agttataagc tcgtccatat ttttccgagc ctcggtaaaa
     1501 atattatcaa aatattctcg agcaggcttt ctaatgcctc cccaattttc tacggcgaat
     1561 tcttctcctt tggttacctt attataccct tccttaatgc gttcgatatg cactaattcc
     1621 tcatcggcca accttttcag cacctcttta cttaaagggc tacttactct ctcatagccc
     1681 tctaagtaaa actgacgacc gtcttcttcc attttaagag cttgctccag aacttcttga
     1741 atggtcaact gacatcctcc tttcttttaa tcaataacca ttgccggtta tcatctgctt
     1801 tactaccttt agttcgaatt aaagcatatt ttcccttaac tttattcccc ttcagttcca
     1861 acaaaatctc atcttcctcc catttatcgg caaggtaata tcctctatcc cagattttaa
     1921 ctttcccctt cccataacct tcggtaattt ccccttcaaa gtcagcgtaa tctaaatcat
     1981 ggtcttccac ctctatggct aaccttttct ctcctgcatt ttggggaata gaacggggaa
     2041 ttgcccagct ttttaaaact ccgtcttttt ccagacggaa gtcaaaatga tgacgagaag
     2101 cccaatgctc ctgaataaca aaaatttttt cttccatttt tcaccccctt caattgtatc
     2161 atgtttttgg ctttcagtga ttagcctcaa atataatatc gaagctcctt tattctatta
     2221 gtagagagag taaggagatg tttcagcaaa ttagagtata gttgatcaca aaaaacagtt
     2281 gtaacctact tcccccgggg gtaggaaacc cattttttaa gggaggttat agagagtttt
     2341 cccctcatac tgaggggtat tatcctcttt ataattttgt cattcccaaa atcagtagtc
     2401 gggaatctaa aggtgtttgc ccttaagtaa tttggattcc tgatagaacc attcaggaat
     2461 gacgggcaag gggagggtag agacattcag gaatgacgga caaggggatg atagaggctt
     2521 tcaggggtag gaaaaaacgg ctcgaaagtt accggggtgg cagatgaggc attaaggaat
     2581 aatagccacg ccgttttatt ccggctatgg gcgccaagga aagatgtaag aaaaaaatag
     2641 agaattcggg agttaactgt ggtaatataa ggggaaaata agaggaagga gatagtgaaa
     2701 atgcctgagg tattagaaaa tttaaagagc cttctcaaaa aggcagagag caaaatggtg
     2761 cttctcgtag ccgatggctt aggcggagtt ccccaccccg attttggcta tcaaacagaa
     2821 ttggaagcag ctcgaactcc caacctggat gagttagcta agaagagcgc cctggggtta
     2881 atgactctgg taaaccatgg catcacaccg ggaagcggcc cagggcatat cgggttattc
     2941 ggctacaatc ccgaagaact taccattggt cgaggagtat tagaagctct gggagtaggt
     3001 ttccatcttc aacccggaga cattgctgct cgggctaact ttgccacccg tgacgagaga
     3061 ggtatcatag tggatagaag agcagggcgc atttctactg aagaatgtac ccggttagta
     3121 gagaaaattt cccgggagat aaaagaaatt gaaggagtag agataatctt aagaccgggt
     3181 atggagcata gatttgtcct gattttacga ggagagggtt tgaaagaagg agttagagac
     3241 accgatcctc aaaaggaagg acttcctcct tatgagcccc aggcgctgga tgcggaagct
     3301 gaaaaaactg ctcgagtttt aaagctcttt ttagagaaag ctcatcaaat catcaaagat
     3361 gaacctaaag ctaataccat tctaatgcgt ggcttttctc aatctccaca tattgagaaa
     3421 ttcccggaac gttatggagt gagagcttta gctattgctg tttatcctat gtataaagga
     3481 atttcccggc ttttcggctt tgataccccc gatgtgacag gagattttca ggatgaggta
     3541 aagcttctcc gggataagtg gtttgattat gactttttct ttttgcatta taaagatacc
     3601 gataaagcag gagaagatgg agattttaag aaaaaagtgg ataggataga gcacttagat
     3661 caattcattc cccaggttct cgctctgcgt cctgatgttc ttgctatcac tggagaccac
     3721 tctactccct cccttttgca cagtcattcc tggcatcctt cgccagttct cctttactct
     3781 aaatatgcag gagttagcac aggtacgcgc tttaccgaaa aagaatgtgc tcacggatat
     3841 ttaggacagt tcccgtctcg ggctcttatg agtctaatgc tcgctaacgc cctacggctg
     3901 ggtaaatttg gagcttgaaa aagccaggtg gcgaggcata taactatctt cttccagcct
     3961 cgccactccc cgaagaaact cgatttccat agccagtaga taccactctc ccttttctct
     4021 ctatcatctt tttaacttct aaaagataat tttcccagga ttcttcgata cccttcccgg
     4081 gataaatgat atatctactc tgataaaaag agggggtaag attgggcata aagacgttgg
     4141 ctccgcagga gagtgccttt tctcttcctc cgggaataag cgttcccaaa gcagtggtag
     4201 caggaagatt agaggtttta gtgaggacgc gggtgagagc cagaactttt aaagtgagaa
     4261 ggtattttcc ctcagggttg gaaacctcct cccctaaggg agtatcgggg ttggggataa
     4321 aagggccaat gccaatcata tcaaaatccc actcctgaaa ttttttgata tctaaagcca
     4381 aagactgaag agtctgataa ggaagcccta ccacattccc acttcccacc tcgtatccta
     4441 attctttgag ggttctctgg cactcgaggc gctggtcaaa atcatcatca ggatgaagct
     4501 ttgcaaaaag ctctcgatca aaagtttcct gtttcaggag atatcgatcc gctccggcat
     4561 ctctccagag agcatactcc cagggctctc gctctccgat actcaaagcc accgctacat
     4621 caagtttttt aatccggtga atgagagaag ctaaactttt agcatcgtaa gcgaggtctt
     4681 ctcccgactg aagcacgatg gttttcagcc cctgcagagc gattttttca gcgaggttat
     4741 aaagttcttc cagagacatc ctgtatcttt taagcttccg gttatcccgg cgcaatccac
     4801 aatagagaca atttctacgg caaaaattgg aaaactctac taaacctcga agatgaacct
     4861 cttctcccac atactttttt ctcttctcat cagcaagata gaaaagttgc tcttccacct
     4921 ttctatctct acacccgaga agaatgacta tctcttcctg agtgaggttt tcttctcggt
     4981 ctaacttatt taataagtcc cattcttttc cgctgtttct catttttatt cctactcttt
     5041 ttttccagtt taaaggaaaa gagtaaataa tctgtattgg aaaaattaag gttcttctcc
     5101 caaatggttg atagaaaagc taaataaaga tgaacattac ttgcttttcc gggtaaagta
     5161 tttgtgccag caaaaccaat ccagaggagc aaagtgatcc cccggataaa gcttaatcca
     5221 ttctttgatt tttgtcgagc aaggtgaaaa aacagccttt cgatcaagaa atcaagaaaa
     5281 aaacggtgaa tattactatt tgcccggata aacattatac aagctcatta atcattaaag
     5341 attgattcct ctgcaaggat gccgcagatg gggataagag cacactcctt acaaaaaacg
     5401 aaaaaagggc tataaggaga agtatccctc tatggttctc ttcctttatt tcccccagtt
     5461 tcttcccgcc tggaaaaaag catcgatatt ctccagaggt acatcctgag gcaaatcaca
     5521 accagtactc aaaataaaag gagggaaatc tctcatgctt tctaaaaggt cccaggtttc
     5581 gtcataaacc tcttgagggg agccaaacaa cattttaacc gggctcacgt tacccataag
     5641 gagaatgtct tcccctgtta cctgagatgc ttccctcata tctaccagac tatccaggct
     5701 caaacctgtt actcctgaat ctaccatctc ttccagaaga tgaaaggtgt taccacacac
     5761 atgaagagta atctccacat catccagaat tcctaccatt tctcgataaa agcgagaaat
     5821 ggtctggcga aattgctgtg gaccaaaaat taccgcggta gggtctaaag ccatgataga
     5881 atctgcccca ttttctcgca aagcctgagc atatctcata actacttgat gagaaaattc
     5941 caaaacctct tcaaaaaaat ctggttccag aatactttta attgctaact gattagctcc
     6001 cattaataaa cctgacaagg ttaaagggcc tataacgtaa gctcccaccg ggcaggggaa
     6061 ggcggctcgc atatgcttta gggtttctaa ataaacgata actcttccat cacccagaat
     6121 atcaattttg cgaaatttat ccagatcgtc tttgctcttt acgagatgag cttccaccga
     6181 aggagtttcc tctaaaggaa aacgaactgg caaccccaga gcgctggctt ccaccgataa
     6241 gtccatgaga aaaaacatgg catcaggacg aaagcggtca taaagacggc tcagggaacg
     6301 aaattgcacc aagtgattaa actgattttg cttgatggta gttccggtga gctgaagacc
     6361 agagtatcca aaaagaggaa caatagcacg ctttcccttc tcataaaaac ggagagctaa
     6421 atcatacaga gaatcagcat tctttctcat ttccaccacc cacaatttat ataaatcatt
     6481 taattattct acctatttta attcgctctc tttctctggt caagatagta aatggttttt
     6541 tacgccatag gttgttttta ttttttcaga gtgataggtg ggtcgaggat gtaacatctt
     6601 ctcgcaattt tacttcgcta ccaaaagcca gctttttccc tgtatcacgg actgcttatt
     6661 cttctcttca gagcactatg agaggggcga ataccccccc atagtgccta caaatgagca
     6721 taagagccga aagaataagt ataaaaatta aaacttttaa caaagtttat tgctatagaa
     6781 aactatcaac cctttaccgc accaaaagtg aggccttgca ctatatagcg ctgcacggtg
     6841 agagccagaa tagtaggagg aacaacagcc ataagcatac gagtagcaac ataccagaat
     6901 tgaattccct gagtatcctg agtaccagca attaaaacgg tcatgggaac agccttagaa
     6961 taggacataa tgaaggcaaa aagaaattca ttccaggcga aagcaaaaca aataattcct
     7021 gccgcaacca aagcgggggc agccaaaggt agggcaatgc gactgaaaac ctggaaacga
     7081 gagcaaccat ctactaaagc cgattcttct atctcttcag ggaggtctcg aaaggcatct
     7141 ctggttacca aaacagcaaa gggaatgcca aaagtagcat ttatcagtac taagcctacc
     7201 actgagtcaa tcaggcctaa agttttaaat ataaggaaaa aggggataac tactgccgcg
     7261 ggaggtaaaa accgctggga aagaatccac atagttatat cccggttttt aacccggtga
     7321 tacttataac gggctaaacc atacccggct aaagcaccaa taaaaagagc aacaagagct
     7381 gaacacactc caattatgag cgaatttttc aatcctcgct tgatctcttg accccgcatc
     7441 tgaagctcag aacgccaatt ttctgaagtg ggttcaaatt gaacaaaggg aataaaagat
     7501 aatctaaaaa catcagctgg ttttttaatc gaggttatta ccgcccagta aaaagggaaa
     7561 agcatccaga ccacagctac aattaccgct atccacaaga taatattagt ccaccgacgt
     7621 ttctttctca ttagtcgctt ccccctttaa tcatagtttg aaccgtaacg acggaaaaag
     7681 atttgactga ctgccagaac cataagaagc agaatcactg ccgtagccgt agccacgcct
     7741 aaattttggt ccctcaggcc actggtgtaa acattaaaag ttaaagttcg cgtggccaaa
     7801 cccggacctc cattagttaa agcaaaaggc aaatcaaaaa ctttaaaagc ttccacagta
     7861 cgcaaaatga ttactgttcc aagagtggga ctgataagag gtaaagttat atggcgaaat
     7921 atttcccaac ctgaagcact atccaggcta gctgcttcgt aaaggtctcg aggtgcagcc
     7981 tgcagagcag ccaataaaac cagaaaggca aagggagtcc actgccacac atcagcaatc
     8041 aagaccgaaa ccagagccca ccggggatga gaaagccagg gaatagcatt agctcctaaa
     8101 gcgtgtaaaa aattgttaac cggtcctcct tcctcgtaaa ggataatcat aaacatatat
     8161 cccagagcca caggagcggt gaaaagagga gtggtacaaa tagtgcgaaa aaactgcacc
     8221 cctttcattt gccgattaaa aagaagagca agcaataagc ccagacctac agtcagtaaa
     8281 acgcttaaaa ccacaaaagt tatggttacc cggaaagcat tccaaaaacg ataatcggaa
     8341 agaacataga tataattttc taaacctaca aatttagtaa ctcgcaaaaa gcgggaacca
     8401 tgaaagctca aataaagggt ataaagaaga ggaaaaatag taaaaaccag tagccaaata
     8461 atagctggaa aaataaaaaa atgttttccc tgctctttaa gaggaactct ttttttccta
     8521 ttcaacccca gttcaccttc tttctcttga aagaggcggt gagctcaccg cctctttcaa
     8581 tgctttactc gccactataa ccaatggctt ccttataaag ctctttttgt acttccctac
     8641 ccagctgatc ggtaatcctg ttccaatctt gagctgccct gttccaggct tcctgaggag
     8701 tagcctgacc ggtgagagct tccgacatat gaatatctaa actctcaatg taatcagccg
     8761 ctccggcaat tcgaagataa ggaacactac gaggatattc aaaccataga gtataataag
     8821 catccaaaaa ctgagttata tcttcagcat catatcctgt gatggtatag tcctcaattt
     8881 tggccttgcc tgctggttct aagaaatcat aagtagttcc aggatcaatt cctgtccaac
     8941 cccaggccac gttccagtgg ttaatttctg gagtagcttg ccaggaaagg aagtgagcaa
     9001 tcatgtcagg gtcttcagca tagctggata tcactccgtg ccacgaagca ccacacttat
     9061 tacctactaa gtttggctct tctaaggtta cccactgttc actttccaaa tcgtacactt
     9121 ccttgctccc cggaatagga gctactccca gtttaccctg gactacagaa acccgggggt
     9181 cttgagagag cgtgcccaca tctccaaagg taaagcacat agcagcatta ccccgcagga
     9241 aaacgtccca ggcttcccct aatccccatc ccaccatggc agaaggaccg gctttggtga
     9301 gctcagtgag catctctaat gccttgacat gccccggggt attgatcaag ggttccatgg
     9361 tttcagggtc aaaccagaat acgttatgat agcgagtaat tttagctgga tcgtcccctg
     9421 gagcaggcat acagacaaaa gagcctgcta aagcaatgta gttaaagaag ccttgctctc
     9481 ctaccttaag atgcatggta attccaaaat cgtcctttcc atcgccattc caatctttac
     9541 cgttgaaaaa tttagcaatg tccagaattt cttgccaggt aacaggagaa acaggaaaat
     9601 catatccata ttcttctttg aactcagcct gccaccgagg gtcattaaac aagtctttac
     9661 gataataaat taccataccg tcatggtcat tattgaatcc gtaccagtta tctccccact
     9721 gtaatagctc tcggatagga ggagcaatgg attctcgatc ccattcgggc atacgagggt
     9781 catcaaaata tttatcaatc ggtataatcc agtcattggc aatataatct ccataaaaga
     9841 agctcggtcc tactatacaa tcataatttc cggttccagt agcaaagtca gtgaatattt
     9901 tagcttgcag ttctccaaag ggaatttcag caatgtcata agtagctccg gtaagctctt
     9961 cccagtaagg tcgccagaag tataaaggac cagaaattgc accatgagga ccggaagcta
    10021 aaactgcaat ggtgaacttc tttcctttcc agtccggatc aggctgtccc ttatctgctt
    10081 tggcaaaagc ttcatcagca gtcttcttct ctaattcgga catttcttct tgagcaaaag
    10141 ctaccaagct caaagaaaaa agcaataaga ttaccaatcc ccaaaataca cttttcctaa
    10201 aaaacttact catcttaatt taagacctcc cttcgatttt tcaactccga gcctaaaaaa
    10261 caaaacacac tcacctcctt ttttttgaaa tgtagcttct acaataagcc cggatttcaa
    10321 aaattgtaac acacacctga ttcgctcaca agtgcatcat ctggctaaaa tcttgaatat
    10381 tccagtaaat aagttattct cctataacat ctacccacac ccggcgagtg cgagggccat
    10441 caaattcaca gaagaaaatc ccctgccagg ttcctaaaat tagattacct ctttctacca
    10501 ataccgaaat agaagaacct attatgcttg tttttatgtg agcagaagca ttgccctcta
    10561 aatgcctgta aggaaaatta aaaggcacca ctctccgtag gacctctaaa atatcctcta
    10621 ccacgctggg gtcggcgctt tcgttgatgg taactccagc agtggtatgg gggacataaa
    10681 ccactgccac tcccttttga atatgggaag aagtaactac ctccttaacc gaattggtga
    10741 tgtctataaa ctcttcttga gaatgcgttc tcactgtaaa ttcattgggc atttttatat
    10801 ctcctattct cctaacagtt ttttcaactc ctcttcagga gtaaatacta cttccttccg
    10861 gtatagctct tctcggtttt cctctgaagt agcccaggga ggaagaggaa actttaccgt
    10921 aaggggaata cgcaaaaaag gctgatcaat gattacctct cccggcaaaa gagtagccgc
    10981 cttatcccgt aaattccctt ctaaatgagc gtactcttct ttgtgcagct cggccaactt
    11041 ctgcctgcct accactgtag ttgctgcctg agttattacc cggtagtcta cctgagaagc
    11101 agtttgttct gctccaatta aaaccactcg aaaagaacgt cccctctctg ccacatctcg
    11161 aaagatgttg cctaaaggcc ctcctccggt gcgaggagca tatttattga gctcatcaag
    11221 gtaaacaaaa actggttcag ccaaacgctc tctctctttc cagtgcatta tctctcgcag
    11281 cactgctccc actacaaaag cctgcccctg ataggtgagt ttagaaatgt ctaccactga
    11341 tattcctccc ggacggttcc aacgcaggtg atagccacct tctggaactt ctttgacttc
    11401 ccggtagggt aagcgagtta tatctctcca cagaagattc aagccagtac ggcgagcagc
    11461 tcgcaggcgg cgagaaatag ctaaagttgt gcgagggtca atctgctctc tctgtaattc
    11521 ctctcctaat ttcccctctc cttctaaaga taagcgaagc atggtaataa gagaaatcaa
    11581 gttattaggc aaactaaagc tttccagaat aagacggggc tcttctcctg cagcctgtaa
    11641 ataaactcgc actatttcct ggtcggttaa cccttgcaaa tcaattttca ccggataact
    11701 accgctgcgc aattctcggc gggcatctgc aaccagacca tcgtattggt ctcgcaagaa
    11761 gtcttcaatc acagtaacag ttaatagaaa attggggttc tgagaaagtt cctcaggatc
    11821 aaacatcaaa ggcaaaagtc ccaattccac aatatccaga atatcccacc cgtaggcctt
    11881 tacttctgga cgactgtggc gaatatgggg agaacctttc tcattgcgag gaggaacata
    11941 aacctccagt tcagagaaag gatgcggttc tacctcaaaa aggcgaaaca tttcttgcca
    12001 ttcggcaaac tgccttccct cttctgagtc tttcttctga gcccttttcc actcttcatt
    12061 ccagtaatcc agaaaaagaa gactctctcc cttaacattg aaaactaaag aacggctgtt
    12121 aactaattgg ttagaaaaag cattgtcctt gacataacga gagtagttca tcaacatcca
    12181 cagcaaaaaa gtagcataag aggttttggc tgccactcca gattgaccgc taatattgat
    12241 atgggctcca ttgtctccca atatatattt aaggtctaaa taagccacgt ctccggaagg
    12301 taaaatcccg caaggaagag cccttttccc ctttcggatt tctgaaaatc ctaaagctac
    12361 ctccagctct tctccgcaag ccacaaacac tttttccccc gcagggggag gaaaagaata
    12421 agggacagga acgaggtctt cctccttttt ttctaaaact ctggttacca ctactttccc
    12481 cagataaatg ggcactcctt gataaattcc ttcgttataa gcttcttcct gataaccggt
    12541 ggagatagta ccatcccact tagctttcac ttctgtaatc atcccgtaag catgaaattc
    12601 tctatctccc caacgataat ctactctcac cacctggtcc agagcaacag tagaagaata
    12661 agaagaatta actcgaacaa agaagcagta aggattaatt tcttctaaac cggtgactgt
    12721 gccaataaac tcactcatct ttttccttcc tccccaaagt tttccgtatg agcccctgca
    12781 caaatggagg agacgaaaaa tattgcccca ggtgctcctc cagagcaatt acaggaaaaa
    12841 tattttctgg tagcctttta gccggaaaat cagcagtcaa aaatggtaaa agagagacca
    12901 ctccatcaaa aaacagagaa agattatctt tttcctctgt ccatctttcc ttattcagga
    12961 gagtttccag gcgcactaat cctttccagg gataattatc atctctttcc accaggcgtg
    13021 tatagcaaaa aactcgcagt gtaggtgagt ccttctttag ataagcgctg aaaaaaggag
    13081 tccgctctcc tcgctccaat ttaaacaaag actccaggta ggaatgagga aggtgtaaat
    13141 cttcaactct ttttaccaga ccgcatggtc ctaccccggg ttggaattct ggatctccct
    13201 cgtgtagcgt cccatctttt atgatacagg aagagcgaga aagcaagtct tttaccacct
    13261 tgctctctaa aacctgcata gcactgaaaa tggcctgctg agccgcttct aattcttgag
    13321 gatatcggga tgcacgagcc gattcccaaa catggaagac aaatcccggt cctaaagaaa
    13381 gttcgcgaga aaccatttct cccgaaaaaa gaccctctgg cgctcccaaa actctctcca
    13441 atttaggggg attttgggga gaaaaaccta aagatagacc cctatccttc tgccaccagg
    13501 caaaacctgc tgccacttcc cctaaaagcg cagttttcat caacactata attcgagcat
    13561 ggattcgcat tcttccatct actaaatata tctcttctaa acccggggag gggcgtttcc
    13621 ctgaccagga aggaaagtct ccttcttcta tctcccatag ctcagaagca gccagagaac
    13681 catcattctc ttcccggaaa aagcgaggga aagaatcttc ctcataaaac caatggtcga
    13741 cttgcgccat tttttctcgc aaaaaatcgg ttaaagttaa ataacttcct cgtttctctt
    13801 ccaaaaagct ttccctcccc tgacttctaa atgcaaagga aaataatgag caaaagaggg
    13861 gtcatgagtt actatcccca ccattcgccc atcctgactt agacgtaaaa tagcatcagc
    13921 cactaattct aaatgttcct ggtctaaagc agaaaaaccc tcatcgataa aaagacaatc
    13981 tgcccccctt tctctgccta cctcccgaag atgaatacgg gataaagcca gagctaaaga
    14041 tagagcaacc agggtctttt ctccaccaga gcattcgctg ggtaaacgct cttcccctcc
    14101 ataattgtta tccaccacca ccatctctgc ttttccctct tccatcttca ttcgtaaaaa
    14161 atagcgtccc tgagataaat ctcttaagaa cgaagaagct tcttcttcca gttcacgaaa
    14221 gagaaagccc agaagataac tacgaaaacc ccgggcctcc agagctttat ccagctggta
    14281 gtgaagttcc caattttttc ttttttcttg tagcccttcc cttagcctgt ctcgctcttc
    14341 tattttagct tgtaagttct ctatctcttt atccaggcta cccttctttt ctctgatttt
    14401 tcccagctct ttctgcagct cttccaatat ttctttctct tgcatcaatt gctcttctgc
    14461 ttcctcttct tttatctcca actgctgtat taagctaatc tttttctcct ggcaatcttg
    14521 gagagagttc tctgtttgtt ttatttctcc ccctaaccta tgccatatct cttgccagtt
    14581 acctgccttt ttatcccgta actgctcaaa atattctgca ctccaaccca ggcgagatag
    14641 ctcttcttga aaaacctttc tttttttctc ttcttctttt tgaagctctt ctccctgttg
    14701 cagcaactca gcaaccttct cttctgcttc ttctttgttc tgccaaagga gctttaattt
    14761 ctcctcgctt ctgctttctt cttttcccag ctcttctaat tccctcgaaa gagtttgtaa
    14821 tcgcttcgaa aatttatccc acaaatcact aaatgcttct tctggaaacc gatcgtcctc
    14881 ccatcgtaaa acagcaaaaa tttgagaaag ttgttctctt ttttcggtta agaatttttc
    14941 ctcttctttt atgttctttt ctatgctctc tatctttact cgctcttcgt ctttctcctt
    15001 tatcctgctt tctattttag ctttcttgac ctctctgttt ttctccaatt gataaagatt
    15061 aaaaagtgcc ttttctctct ctcctctttt ttccttcaaa aacccctcta tactttctgg
    15121 ctttacaaga ggtggtaaat caatgttctt tagaacttct aaaattctgt tcttttcttc
    15181 ttctaatttt ttcttttttt ctaatttttc ttcttcttta ttttttaact ctgctatttt
    15241 gctttcttcc tgagtttttt cctgacggag agagctttcc tcttctacta atttttctcc
    15301 ttgttcctga aaagaatgat aaccttccca atctacctcc ggaaggtttt ctgcctcaga
    15361 aggaggaggg acattgctac cacagagagg gcaaaatcct ttttcttccc actctttttc
    15421 caactcccaa agagcaaact ttttaaccat ctggcggtaa tcttcttctc ttgctttctt
    15481 ttcttcctgc cacagtgaca gtttttttct cttcttttcc cactctctct ctaactttct
    15541 atatctttca ctacttagct caatgtcttt ttgtaaactc ttgagttcta cagcaataag
    15601 agaaatttct ttctcctttt ctcctaactc tttaagaaga ggttctattt tttcttctgc
    15661 tttttctact ttttgaatta tttcttcttc ttttttaatc ttctcttttt cttcagtaat
    15721 ttttctctct aattcctttt cttctttttc ttctttttgc agaattattt ccagttcttc
    15781 tatttttttt cttattcttt ccttatcctt ctctaattcc tgcaattctc tctttatggc
    15841 tctggcctct tcagctaaag gaagagcttc tcggcgaagt ttatcttcat aacttgctaa
    15901 gttatttttt ttagtctcct gctccttaat tttttctcct atttcttgca aatttttgct
    15961 cagttgagca atttcttctt ctatttttgc tttctttccc aaccaagctt ttttatcttt
    16021 ctcccatttt tcaactcttt ctactactat ctgccagggt tgccagaaag aagcttttac
    16081 ttcccaagct ttttggattt cccggtcttc ttgagctttt ttataagctc tatctctctc
    16141 tttttctaaa tcatcatgct ttttttgtag ctctttttcg ttatttttta tctctgtgag
    16201 cctgttcaat agctcggttc gccttttttt gtctttttcc tctccagcca aatctgcagt
    16261 tttatcttcc aaatctttct tttcttcttt agccaagttt aattgctctt ccaagttttc
    16321 tttttccagc tcctccaatt tactctcttc tggaagaagc tctctttcta aagagcgtaa
    16381 ttcctctttc actctctccc gaattacatc cagcaactca agaccaaata gactgctcaa
    16441 agtcttaaat ctctccgcag gagtggcttc tacaaaagaa agggtttctc cctgaggcaa
    16501 aaataccgaa gatttaaagg tgagataatc aaaacccatg atttcttcta ttttcttatt
    16561 caccagctgc tcccctgaag ctaaacccct ccagacgcca ttttcctttt tctctaaaac
    16621 tgccacccca ccccggggag aatactctct aaccacctgg aactcattac cccccaggag
    16681 gaatttcaat ttcactcgca ggggaccttc gggatatcct cgatgaatca attctctttt
    16741 gcctcgctct aaacggatac ctcgcccgaa gagagcaaaa aaaatagctt ccagaatgga
    16801 agatttacct gctccatttg gacccaccaa aacaaaaaaa ctggaatcaa aatcaaggga
    16861 aagagactcc aggcccaaaa acttttctac ttctaattca agaggtctca ctaatctctc
    16921 ctgcttcctg gtaatagcga taaaaaagct taaccacttc ttctgataag ttttgctcct
    16981 ggcaataacg agaaaatagt tgaggaatag aatcttcgtt caccggaagt ggttcttctt
    17041 cgacttcctt tttctctcga ggaataactt ccaccaccac tatttgccag ttttctcccc
    17101 ccatctcttt tagcttattg gcaagagccg gggtataaaa atcttccttg atgcgcaaac
    17161 gaatataatt ccttttcccc gtcatatctt gagaaatttc ctcataaatt tcctctatct
    17221 ctttttcttc cttcaactcc caatcccaca aatgcgcgcc agataaaaaa taaggttcca
    17281 ccttgctttc tttcccttcc tccaattcca ccagcaaaaa acttccctct tttcccacat
    17341 gttcaaagct atccactacc agtgaagagg gataataaag aggacagggc gagcgcaaaa
    17401 aagagaaggt gtgcaagtgg cccagtgccc cgtagttaaa agcagaagga atatcagctt
    17461 ttttcaaaaa cacttcccgg tgagggttag cttcagcgta tcgggctaat tcttcaatcg
    17521 tagcatgagc taccaggatg ttccagcgat caggaagaaa acttctttcc cactttttca
    17581 tctcctgcct cagtagctct cgagctcgat cctggatctc atctccccgg taatcaccca
    17641 aaaggcgagc cagactcacg taaggaagag gaaaaacagt gacagggatg ccctctattt
    17701 cccgaggagt ataatcacgg atgatgtaag cttgaggagg aaaaattttt ttcaaccctt
    17761 ctatagcata ccaatcatga ttgcccatca cccacacaaa aggcgcgata tccaccagac
    17821 gtaaaatggt atccacagcc agcttgatgg tttttttgtc gggatgatga gaactgtgaa
    17881 aaaggtctcc agcgtgaacc acgaggtcta ccttttctct ctctgctacc cctataagct
    17941 cttccagagc ttctctttgt tcctctcctc tatctacctc ccggcgggtt ttccaggata
    18001 ccaggcccaa atgccaatca gcagtatgga gaattttaat tttcttcttc attatctttt
    18061 ttagctccaa acccttgatt gttgcagttt aaaacgaaaa aaccgactac tattgatcac
    18121 gatttattat atcatagaga aattaattat agttaaccgt aaagcccatg gtaatgtccc
    18181 tcttggcact atgttttcct tccatgcagt agaattcatc gtgtggcaag ccaccaagaa
    18241 gtcattatga ccctcgaaca tgtcttttgt ttttttgggg ttatgagggg tatgttttct
    18301 cctttatagt catgacaggc tttgcgggtg ccatattttc tccaaagaca aatacaatgc
    18361 taattcattg tgggggtatt tagtttcttt tcattattgt tttaggtctc ttttttccat
    18421 ccaatttcaa atagtgccat ttttagccag aaacaactca tcagaaaacc ccttttcttc
    18481 tcttaacctg atatatttat tagtggctac tttcttcttc cgttatgctt taattatgaa
    18541 aggtttttac gggatactat aaaaatgagt ttttgttttg ttcgttattt gcgctctaat
    18601 aatttatttg ttctgtgtta ttttatacac ggtcttgtta tggaagggag ttactgaatc
    18661 gatgtgtctg ccacatcatc tgaaaggagg atttagctga ttaaaagcag ttggtttgtg
    18721 gaagtaaatg tttttatctt gcttggcttt ttgcttggat acgggttcag actcgagggt
    18781 gactatcttt ttgcgcttga agcatcttct cctgattatt tgcttgattt acaacccatc
    18841 cgttctgacc gatttactat ttattcccct tatccagaag aagactttcg gtttaagggg
    18901 caaatccact gtcacactca agcgtcagat ggtgatgata ctctgcagga agtaatggag
    18961 gcataccagt ctttgggata ccactttgtg gtgattaccg accatgatca gataacaaaa
    19021 gctcctgcag ttcccgggat tctatttatt cccggggtgg aaaaatcttc ttcttcaggg
    19081 cacattttgg gaataggagt gaaaagccaa actgacgaga tgcggccgga taaaattatt
    19141 gaccatattc aaaaggaggg aggggtagcg atttatgctc atcctaaccg atggcctcta
    19201 ttcagtgggt ggactttgag agagttatta tctgtacccg gagcttgcgg tatagaggta
    19261 attaatgcaa aaagatggag aactaattcg gagaataaat gggacgcttt gctgacccga
    19321 gggtatcgaa tctgggggat ggcaggagat gattgccact ctatagccag ggaagcgaat
    19381 cgagcctggg tagtagttgc agctcccgat ttgcgggaag atagcatcat tgccgccttg
    19441 cggaacggcc acttctattc taccgaaggg ccggacctct cagtaaaagt ttatcctgat
    19501 aaaattgtgg cttccacaag tagagaggcc actatcgagt ggattgcaaa agggggagtg
    19561 aaaagaaggg taatcagagg agtcaaggaa gatacctatt ttgttgaagg agatgagggc
    19621 tacataagag tagtggtgat taggaaaagc gataaaaaaa gagccttagg gcaaccaatt
    19681 ttcattgaag gagcaggggc aagctcgaag ggttagccct tttatttaag tagatccatt
    19741 tttctatact gcggttatga tttttgattc accctatcaa tccttctttt gcgaaactgt
    19801 aaaaatcttc catatttttg ccaatgatta catggtctaa cacctttatg cccagaagct
    19861 cgcatgccga tacaatacga tttgttaact caatatcatc ctgggagggg gtggtttctc
    19921 ctgaaggatg attgtggacc aggattacag aagatgcaga ctttaaagat gcagccctga
    19981 caatctcctt agggtcaaca atagtcgcat taacactgcc cttgcttatt tcaacattat
    20041 caatgggttt atttttaatg tccaggagaa tgacgttaaa aaattccttt tctttgtccc
    20101 tcagataagg agcataatat tcagaaacat agctaattac atcatctggc ctggttaatt
    20161 ttttccgggt tgaggctctt tcctggaaaa gccttttacc cagctccagc gatgctttga
    20221 tttgagctgc tttagcggga ccaatccctt caattttaga tagttcagaa attggtgctg
    20281 catctatcgc tcttaatgac ccaaaatggt taagcaaggt ctggcccagc tcttcagcgc
    20341 tcgagccatt tttccctgtt ctcaaaatta ttgccagaag cttggataac ggcagatttt
    20401 ccgctcccag ccgcgccagc atttctctcg gcctttcttc ttttatccat tcttttatag
    20461 gacgagttgt acgacgctct gctacctgtt ttttaatttc ctctctcaac gtttcgcact
    20521 catcacctgt ttcgatattc aattgctcaa tgatttcttt cttttttcct tcggttagta
    20581 tccctttctt agaacatctc aaaaaaatct ttcccttttt gctgcaaaaa ggataaatag
    20641 cattcgcaac cataaaatcg aggagctctt ctccggggct attcatgtca tcatcaatct
    20701 cctttcaact ctcttttgta aacttaaatt tagctacatt tcttgtgcca caacaatttt
    20761 acgctacata cagaacttct gcagacttca gtatttcaga ggcaattcta tcgatttcct
    20821 cgccacagaa gaggtcttct cccccctatc tcgtgaaaag agaatcaaca agttttgccc
    20881 tggtgtctgc ctcgcttaat gcttctattt cgactccctc gtattttaca caatatctca
    20941 taaattcgct attttaataa ggattctggg ctttgtgaga tttttataag tggaatggca
    21001 aaggaaagag ctaataaaat tagcttaaaa ccatcaaaaa gacaattggt ctttcctttt
    21061 ttgagtactt ttgctaaact aatcacagaa atgaaaaaga aacaattaaa gctaatattt
    21121 cttttactgg taattttttc cttattttct gtttctgtac aggctcagga tgttgtggcc
    21181 tggagagatt tctctcaaga gctaactcgc cttctccagc ttccttcagg aagaattcct
    21241 gattttggag aaggagaaaa gcctataacc cgtcttgaag cagcaaaaat ggtgatccag
    21301 gctctttctt atcaagactt gctctccttt gtagatacaa aaagcgttgc cttttctgat
    21361 ttatccaacc tctcttccga agaccagaag tggatgattc ttgcctgtac tttagagcct
    21421 ccccttttcc agggagatag cacagggaag ctgagaccta aagaccccct cactaaaagt
    21481 gaattccttc aactaaaaga aaagatatcc cgctatgccc ggggagatgt aagctggcaa
    21541 agggagaaag ttatcaatca gtatattacc ctcttagtaa agaaagaagg gttctctcct
    21601 tctcccagtt tagaaaccac acaggcaaaa ggaccgttaa gtggacaggt aatgctccaa
    21661 gtaggagcat ttcaagacca ggagagagca gagagaacag ctacctggct caaagaactg
    21721 gggtatacaa cagctcaagt gaaaaaggag ggagacctct tcaaagtaag aataggtccc
    21781 tactctaaag aggaggcctc tcaagttagt gctcgtttac aaaagcaggg ctttgtatct
    21841 tatcaaataa caacagacac tactccaact gttaccccta ccactcctgt gggacctttc
    21901 ttcagcgtgg gattgctgtt tgaccctaat tctcctctcc gattagaaat agccctggct
    21961 caagataaag tgttagagaa agaaaaaacc agcgaaattg cccaaagaaa aggagcaatt
    22021 tttgctgtaa atgcaggttt ctttgcccag gatggcgatc ctttaggaac tctaatgata
    22081 gatgggagaa tcctcagtga gcctcaagaa gggtggtata gcgtcggtat caccgaaaat
    22141 aaccagttgc ttttcggaga gctaaagttt aaagcccagg cacaggcaca aaatggtgcc
    22201 acttattcca ttaccggaat caatcgccgt aataatggaa aagaattagt tttttacgac
    22261 catttcttag gggagaaaac tccaaagcaa gaaggaatag aaagtattat agttaaagga
    22321 aaagttatag aaactaaaag tagccagggg ggaacggtta ttcccactga tgggtttatt
    22381 ttacaaggca ggggagaagc cagccagtgg atgtggaaaa atctcatccc cggctcttca
    22441 gttaaagtca aaatgtcgat ataccctaaa gatgaggatt gggagagatg gaaagacgta
    22501 aggtacattt taagttctgg ccctcttctt ttccgaaatg gacagccggg accgttcggt
    22561 aatttcaacg aaaaaatagt aactgaaaaa catcctcgca ctgtcattgc ccaaactgaa
    22621 gaagggaaga ttcttttttt agtggtggat ggcagaaatc ctgctcatag cgtgggacta
    22681 actattcccg aattagtaga agaattgaaa aattaccggg tagttaacgc gctcaacctg
    22741 gatggcggag gctccacaac tttttatctt caaggtcaaa tccttaatca accttctgac
    22801 cctctgggag aaagaaaagt ttccaacgct ttcattatcc gttaattaat tcaaagtgcc
    22861 ggagttaaat ctcgctctcc tctcactatt tctccaatgt cctccggttc ttctaacaca
    22921 aattcggggt gaagctccag aactttctcc agctccagtg tcccccagga agccagagca
    22981 aagggaatca aagctttctt tgctgcttga atatcgtaaa tgctatcacc tataaaaata
    23041 gcttccttgt tctttacccc caatctatcc agagccaaga gaagagggtc gggatgaggt
    23101 ttaggacgag gggtatcctc agcacaaatt atagtttcaa aaaaatggtt tatcatgaag
    23161 tggtcaaact gataagacat ttcagagcga atcttagaag taaccagtgc catttttagc
    23221 ccttcctggt gcagttcttc tattacttct ctaactcctg aaaaaagtcg tacttccctc
    23281 ataccttccc gaattaattc ctgccagcga gccacaaaag ctctttccat ttcgggggga
    23341 atattcaaag cctttaatgt ctcagggctg gggatgccca cagtaaaaac aaaaaaattc
    23401 tctggcttct caacccccat aaactcccga aatgtttgag agatgctccg taagaataga
    23461 gggttggtgt cagtaatggt tccatctata tcaaaaataa ttgctttaat caatttatca
    23521 ctctccaatc cttagatatt ataatagtga tgtcaacaga aaaagagaaa acagcctaac
    23581 ggcgtaagat taaaagatta aaagactact gaagatgagg aaaacatctt cctcagtgga
    23641 ttcccgcctg aatttcggga atgacagagg agaggccatt cctgagcggt tctacgagga
    23701 atctaaataa tgggaggaga tgctcctatg g
//
LOCUS       DAOPSS010000008        22848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_1861_length_22848_cov_9.692362, whole genome shotgun sequence.
ACCESSION   DAOPSS010000008 DAOPSS010000000
VERSION     DAOPSS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 22848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22848
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_22848_cov_9.692362"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..495)
                     /gene="lspA"
                     /locus_tag="PKN31_01145"
     CDS             complement(25..495)
                     /gene="lspA"
                     /locus_tag="PKN31_01145"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOA98566.1"
                     /translation="MNSKPGKGNIRWLWFSLAIALALIADQLTKQWAIKNLAEDSIVL
                     IPGILTLTLRHNFGSAFGWKIMSKEGLALITVFITIVLLWIFIKIAYIRGISLFLAGA
                     WGNLLDRWRFGYVIDFIEPSFWATFNLADVFIVMGIILILLSLWFDEKRENSSL"
     gene            complement(492..1061)
                     /locus_tag="PKN31_01150"
     CDS             complement(492..1061)
                     /locus_tag="PKN31_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOA98567.1"
                     /translation="MSWWKPEEIREIDFNRSIRGYNEHEVREFLEELASQIENMAVEM
                     EKILQDNRELKRRLAGREEKLAEMEAQMEEWEKQAEVERELAKRESQMVLKEAEIRAQ
                     KIIEEALERKKEIERGYQDLLEQYRLFQIRFRSLLQTFMDSLEWKEKDWDTGKALEEG
                     NKEEKKEEGGEIARFSFQDFQEDSRLRKS"
     gene            complement(1058..1867)
                     /gene="proC"
                     /locus_tag="PKN31_01155"
     CDS             complement(1058..1867)
                     /gene="proC"
                     /locus_tag="PKN31_01155"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOA98568.1"
                     /translation="MEDSILFVGCGNMGSALIRGLCRDNWHKKFRFFLLDQVVEKART
                     LASSLDLEWVENLNNLEAPRFVFLAVKPKDVKKVLLSLRNFSQSVFVSVAAGVKIKDL
                     EELIPQQAIVRIMPNLCVEVGEGVVPVCFSPMVKTEEREDLLFLFSSLGWVFEAEEED
                     LDLFTALSGSGPGFIALFIEALADGGVKIGIPWERSLKIATQTVLGVASLLKSRDMHP
                     AQLKNWVASPGGTTIAGIKKMEEGAFRGLVMGGVEEAYRRAKNIEDEEEKK"
     gene            complement(1873..2604)
                     /locus_tag="PKN31_01160"
     CDS             complement(1873..2604)
                     /locus_tag="PKN31_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOA98569.1"
                     /translation="MGAFTLEKCKALAGGRYGVRENWEAVKEKITIFSQRAGRNPEDI
                     KVIAVTKGVEVSKIEDAISLGLIYLGENRVQEALKKMPLLPEEVEWHFVGRLQRNKAK
                     KVVEFFQYLHSLDSIDKGEELEKRARDLNKKLPVLIQVNLTGRETQGGVREEELLSLV
                     YHIAQFSHLELRGLMTIGPVGGEEQEVRKIFSRLRELKEEVNREMPRITSFQELSMGM
                     SDDFPWAILEGATMIRLGRAIFGER"
     gene            complement(2547..4109)
                     /locus_tag="PKN31_01165"
     CDS             complement(2547..4109)
                     /locus_tag="PKN31_01165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA98570.1"
                     /translation="MVESAKNYWEEEQGIFPSSKVGKFLFALCFPGDYSLGMTNLGYQ
                     SIIRLLFEAPDWRGERFFCEYGSRSLESGLPLNSFDMIGFNFSFELDILNLLSLLQEG
                     GIPLFARDRKEKDPWVISGGPAATLNPEILAPFMDLIVIGEAEEIFPLILALWKEGEE
                     KGKSREEKKKILGNIPGVYIPGGVKPLYQGGELQGFEAEEGFSFPVKRQWVDINEFET
                     RTFIYTPFSYFQGASLVEVSRGCRYHCSFCAGRNIYHPLRNRSLELIEKMIDHVSPWS
                     DKIGLVGADLLSHPALEEIIKYVFKKNKKLTFSSLSALSLVRKKNLGELFCEGEVETI
                     TLAPESGSFPARMVLGKGLSNEEWIDLIKELLEQGIKRIKLYFMLGKPEGGVEEDLEF
                     LTKLVARIKNPQQVLVSYSFLIPKPHTALSDWRTPSIRDWEREKRIWERGLGKLKIPF
                     SGESPRLAWIQLLLARGDRLIGEKIPQLLSHPHPWSFRAWQSLLGELGRDDEWVRSPW
                     RNVKPWQVVDMG"
     gene            complement(4114..5160)
                     /gene="ftsZ"
                     /locus_tag="PKN31_01170"
     CDS             complement(4114..5160)
                     /gene="ftsZ"
                     /locus_tag="PKN31_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOA98571.1"
                     /translation="MRSNSQRLRNGKVNIKVIGVGGGGGNAINRMIQAGLKGVGFVAM
                     NTDTQVLERSLADVKLQIGAKLTRGLGAGANPEIGRRAAEEDKEKIFEILEDVDMLFV
                     TAGMGGGTGTGASPVVAGLAKELGILTVAVVTKPFTFEGRKRMRQAEEGIALLRERVD
                     SLIVVPNDRLLQIASPSTSVGEAFSMVDNVLLQGVKSISDLINIPGIINLDFADVKAI
                     MEGAGTSLMGIGRAGGENRAKEAAYLAVSSPLLEIPFSGAKGVLFNVTGGPGLSIWDV
                     NQVAEVITESASEEANIIFGAIVDEKMKDEVEITIIATGFDHGEEEKEERVSEIADSF
                     SFDDLDVPAFLRKK"
     gene            complement(5179..6441)
                     /gene="ftsA"
                     /locus_tag="PKN31_01175"
     CDS             complement(5179..6441)
                     /gene="ftsA"
                     /locus_tag="PKN31_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOA98572.1"
                     /translation="MKSWRSLDSHSPILGAVDVGTSKICTLIGQKKSSAIEVLGVGLS
                     SSRGVRRGKIIDINKAAGAIRESFSLALEIARAMPDLVYAGIAGDGVSSFNLENEVFL
                     NKGGREINARDVDKVLEGSKAGVNLEGKRILHLLPQEYIVDDQTGIADPAGMIGSSLR
                     AKVHLITVDENQFHNFSKSFQQAGLEVHRLVFQPLASALAVLHPMEKEMGITLVDIGG
                     GTADIAIFYGGSMRYSGVVPLGGESITSDLAVGLRTSRDEAERIKLHHGCAFSRHIPD
                     DEVIEAKNLGLSSLQTVKRKYACEIIEARVRELFLLIRRAIKSSGFYPLLRGGVVLTG
                     GSALLEGIAELGSSILELPVRVGSPESKDHVGMIQTVSHPMFSTACGLLQFGLEEATK
                     RGRKSLVLDLENRGSKVIDWIRDFFMLD"
     gene            complement(6438..7070)
                     /locus_tag="PKN31_01180"
     CDS             complement(6438..7070)
                     /locus_tag="PKN31_01180"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOA98573.1"
                     /translation="MFSLIFQTGIFKVKRIEISGNQWLQEEELLKVVNIPLGSNIFAL
                     RPWEIEKRLEELPQVKEARVRKSFPYLVQIEIKERQPEAWVEVGDEVFCVDEEGVEIP
                     ASSTDLVRVYLPENRKSLLLESLDLIKVWKKEFELPLSAVTVENDRLFILQLQSGIFI
                     RCEGVNNLKKKSAILKPYLRDIAVKYLDVGGFDLRVGDDLVIVPSEEDAF"
     gene            complement(7141..8052)
                     /gene="murB"
                     /locus_tag="PKN31_01185"
     CDS             complement(7141..8052)
                     /gene="murB"
                     /locus_tag="PKN31_01185"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOA98574.1"
                     /translation="MLSRWRKIINQLENIPHLSIIPQPEMKNFTTWKVGGKALALVEV
                     RNGGCLDEVISSCEREGIKWKILGRGSNVLVKDEGFPGVVIKLGGEFCELKREGNCIE
                     GGAGAPLSHLVSFAREECLGGIEFLVGVPGTVGGAVRINAGCFGKEIGPLVKKVEIRE
                     ENGNREWLNRDDLFFSYRYSNLKKEKIVILKAVFNLFPEEQEILEERIREFCYLRKEG
                     QPLEWPSAGSVFLNPEGNSAARIIEEMGFKGLRLGRAQISSKHSNFIVNLGGAKSWEI
                     EYLMNWVQKEVYKNKGIWLEREVELWP"
     gene            complement(8028..9413)
                     /gene="murC"
                     /locus_tag="PKN31_01190"
     CDS             complement(8028..9413)
                     /gene="murC"
                     /locus_tag="PKN31_01190"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOA98575.1"
                     /translation="MDKTHYLLPRNLHFIGIGGAGMSSLALIAHEQGYVVSGSDITEN
                     EAIARLKGKGIRVFLGHRLEQVRGAEAVIVSSAIPEENEELKEARRLNLPIIPRGEML
                     ASLVNSKKGIVVAGTHGKTTTTSMISLLFEMAGLDPTVSIGGELEDIGGNAKAGRGEF
                     FIAEADESDGSFLKLNSFCAVVTNIEDDHLIFYGNMEEEKKAFIRFLHQIKQEGFGVV
                     CQDNPTVRSILPYLSSLEILTYGMEEGDVRGKIKEKKKDGFIFQVDWKDKKLADFELR
                     VPGLHNVNNALAAITVGLKLGIELNHLKKAISSFRGVKRRFEKIGRAKGALVIDDYAH
                     HPTEMLTVWQTALDYAGGNLIVIFQPHRYTRTFQLYREMARVLEKFQQVFILPVYPAG
                     EKPIPGVSAELIYQEMKKDGFEKVQLVESSEDAARECLKILKPEDLVVTMGAGDVWKA
                     GWILCSQDGGK"
     gene            complement(9382..10509)
                     /gene="murG"
                     /locus_tag="PKN31_01195"
     CDS             complement(9382..10509)
                     /gene="murG"
                     /locus_tag="PKN31_01195"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOA98576.1"
                     /translation="MDTIFIAAGGTGGHIFPSLCIAQEIRKRGFQVIFLGTKRGMEEN
                     LLKEADFPLLFVRARGWNRKWDFSFFSVLMDNLIGFFQTAFYFFRYRPRALLGMGSYL
                     SLLSAIWARVLGVPIYIHEQNIYPGLSNRLVSRWARKIFISAPEAIVYWKDREEKIDV
                     VGNPLREEVMDWKGKKEEARQKLGLKKDRQTILVMGGSRGSEVINTTFLGTMDWLEKK
                     GLQVIHITGEEGFEHTKEMAEKFSSPYLVYKFLPQPGIAYAAADLAIARAGANTIFEL
                     FWFELPSVLIPYGDATDDHQLYNAQWLEKQGLARVIEEKNLTSSLLIEEVEKILISSS
                     CVFSDLRGKYLRESASQIAQTIIEELKRGKGIGQDTLSLTP"
     gene            complement(10510..11664)
                     /gene="ftsW"
                     /locus_tag="PKN31_01200"
     CDS             complement(10510..11664)
                     /gene="ftsW"
                     /locus_tag="PKN31_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOA98577.1"
                     /translation="MTWNKITLPVNDERLSFKTRWWWDIDPYLFWSILLLLLLGIVMV
                     FSASMTTALYAYGDVFYFLKRHLLGILLGFLFLTIFTFVSLDRLRSINRFLLLATFVS
                     LILVFVPGVGRVRGGSYRWINLGALSFQPSELAKLSIILYLADALANYKERVEDFWQG
                     IMPFMILVGLICLLVLIEPDLSTAFFLFLVAFLMLFLGGSKLQHLLLILIVLIPSGVF
                     LILFGGKDYWRERIYAFVDPWKDPLGRGFHIIQSLIALGSGGIVGRGLGESRQKFFFL
                     PDRHTDFIFAIIGEELGFIGTSAVVILFIIILWRGWKISQGSQDEFRGLVAMGVTLSI
                     ILQAFINMAAVLKLLPVTGVTLPLLSYGSSSLIITLSELGILFNVARGTK"
     gene            complement(11633..12976)
                     /gene="murD"
                     /locus_tag="PKN31_01205"
     CDS             complement(11633..12976)
                     /gene="murD"
                     /locus_tag="PKN31_01205"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOA98578.1"
                     /translation="MQVVLGLGKTGIAVSRFLLEQKEKVLAIDDYLKEEQIPRDIAES
                     ERFEFLSSSDFLQTDFSSISEVITSPGIPPTHPALRALEERRIPVISEIELATRFISF
                     PLIGITGSCGKSTVVFWTKQILERAGWSVFVGGNWGYPLIESLSAREKFDWGIVELSS
                     FQLSRIKKAHFSVAVVLNLFPNHLDYHSTIENYFQAKARIFENQRRDDFAIINFSLSD
                     WSTRFSRLVKSQLVPVARDKKLTEGFYISGNEVFQGEKALFPLSHFSLPGKHQQENLL
                     AAISIALLLGIEDKIVEEAISFLSSLPHRLEKVGSWRGIDYFNDSKSTTPSSTRVAVE
                     SLSCPVVLILGGRAKIDDFSELNSALQSSKVKAAVIYGESRNLIERFIPSHLERYLVS
                     SLEEAVKRARSLAQEGDAILLSPACTSWDQYRNFEERGEHFRQLVYDLEQNNTSC"
     gene            complement(12981..13943)
                     /gene="mraY"
                     /locus_tag="PKN31_01210"
     CDS             complement(12981..13943)
                     /gene="mraY"
                     /locus_tag="PKN31_01210"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOA98579.1"
                     /translation="MNEIKSLIFSLLMVFALGLFFFPFFIRWQEKTRIGQKIKREGPN
                     LHLHKENTPSMGGIIMILSGLLVLSFFQQFTPQIWIILITLLSFAGLGLLDDYFKSFR
                     GEPWSLKARHKFLLELVFSVFILILGFPLFPHYLIVPYGSGIIELGEIPFFLYGLFLI
                     LASTNAFNITDGLDGLAAGCGILTFSFWAGLFFLEGNFPLAQVAIIFSGALLSFLWFN
                     FWPARIFMGDCGSLSLGALMGILALFSGYSWLLALAGFVYLLDTLSVILQVFSFKVFG
                     KRIFLMSPLHHHFELRGEKESKITVRFWIIQGVGVVAALLGIMR"
     gene            complement(13930..15327)
                     /gene="murF"
                     /locus_tag="PKN31_01215"
     CDS             complement(13930..15327)
                     /gene="murF"
                     /locus_tag="PKN31_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOA98580.1"
                     /translation="MKFYPQEIVEITQAEMQKTGKAPFFEGVAIDSRSLKAGQLFVAL
                     KGERTDGHLFVKEAIKKGAPGVLVSRFEDGEEETYVFRVPDTFLALQHLGRGASQKLS
                     GYKIAITGTVGKTTCKSFFASLLSRRFLVEATPQSFNTVIGVASSIANFKENNDYLII
                     EAGISKKKEMEELAEIIDPHMVVFTSFGEGHLEGLGSVEEVVEEKIKLVREKTEIIYL
                     NTDQKWSGLAGEKIEGKKIISFGFSPANHIYLKSFSLDPLTWKAKIEIKMGENDFCFF
                     APFLFPEVAVMLLPAFHLACKLGIGVEEIEGSLRDWQPPQGRGNFFFYQGGIVIDDSY
                     NANPLSSRKSLQFLDFLACHGFETWGVWGDMLELGGVSAEAHREFLRELKKSSLSRTL
                     LVGEEMKRAAEEIAKEEIQEGRFLLFSTSLEVREFLSRYTPRGERWAILFKGSRKLEL
                     EKAIPPEWRENHERD"
     gene            complement(15284..16825)
                     /locus_tag="PKN31_01220"
     CDS             complement(15284..16825)
                     /locus_tag="PKN31_01220"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOA98581.1"
                     /translation="MTIGQFLSSFSFEEGKIENPEEEVRGLVFDSRRVQPGYVFFALP
                     GSNTDGHFYIEEAIKRGATLVVLEKKEFALPPQVKVSWVLVPEGRKALALASSFFYGD
                     PSSSLRVMGVTGTNGKTTTCFFLRAIWEKSGFPCGLLTTARNIIGPKEEEAINTTEES
                     LSLYRHLYEMQKMGIKNAVVEVSSHALALRRVEGIKFDSALVTNLIPEHMEFHRTFEH
                     YFESKRKLLQMVEENLSKSYPRVVVVNGDDENCLKMRKGIGVPYLIYGLNKEAEIRGE
                     NITCSLQQSSFLVKSPWGKMEVVIHFPGIHNVYNALSAISLALFYGLDINDIQEALFQ
                     VHHIPGRWELVEAGQDFTVIVDFAHNWHGLEKSLSIIRDLTPGKVITVFGCGGERDRS
                     KRPFIGETVARFSDACIVTTDNPRGESPEQTAFDALEGVKRAAKNKDLHFEVILDRED
                     AIRQALELAQKGDTVFLAGKGPERFQVYGGKAIPHNDHYIAWKILKEKKNEVLSSRNS
                     RNYSS"
     gene            complement(16822..18513)
                     /locus_tag="PKN31_01225"
     CDS             complement(16822..18513)
                     /locus_tag="PKN31_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOA98582.1"
                     /translation="MISRKFVRRANFLFFLSLFLLGVVAVRVFSLQVLQGKFYREEAL
                     SRLVLEETLPSRGSILDRSGKVLAEDVGVLSISAYPPQMDDPEKIAEELSQLLSLSAS
                     ELQQKFQSGLFWIEITDNAPLYLKEALEEKKLKGISWKEKPVRFYPQAPLFSNLLGFV
                     GKDRQGLEGLEFAFDSYIAGKPGYTFYEKDALGREIPLTTKHLSPQSGNDLLLTVDST
                     IQFFAEEALDQAMEKTKALRGVVIVNNPQSGDILAMVSRPSFDNRNFQDFPSESWKNL
                     AISMVFEPGSTVKPLVMAAALEEKKASLEERFFCPGFVMVYNHRVRDIKAHGEQKLED
                     LLINSCNVGIITLAERLGKDKLFYYLKNFGLGEKCDIGLPGEEQGLLRPSRDWSLLSI
                     GAIPIGQEMMVTPLQLLRALSALANKGVVMQPRLVSKIISPTGEIIEGFSPVSSGEVV
                     SERTASLVLEMMEKTVKEGTGKKAYIEGYRIGGKTGTGQKVGLDGRYDPRKFYSSFVG
                     FFPLPEPRFGIIIVLDEPQGEYYGGDIAAPVFREIAQNIINYAGIMSDEAEVGLF"
     gene            complement(18510..18842)
                     /locus_tag="PKN31_01230"
     CDS             complement(18510..18842)
                     /locus_tag="PKN31_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOA98583.1"
                     /translation="MKRVKSWLIFGLFFIFSLSFLCVTLQALLLEKSYLVEQKRENID
                     NLLATREKLKMEIAQLSSLERIKEVAINQLGMTYPDQTVYMVSSSALGGGEGELVLGK
                     MPEETQGE"
     gene            complement(18845..19741)
                     /gene="rsmH"
                     /locus_tag="PKN31_01235"
     CDS             complement(18845..19741)
                     /gene="rsmH"
                     /locus_tag="PKN31_01235"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOA98584.1"
                     /translation="MNTYVFHQPVMEKEVLHYLLWRKDGIYVDATVGGGGHALSILGE
                     LDEGAILVGIDRDEEAIKMAKETLKNPSLPVYLVKERFSLIDQVLFSLGIEKISGILF
                     DLGVSSWQLDKAERGFSFQREGFLDMRMDLSEERNAYQVVNEFPEKELADLIYYYGEE
                     RFSRRIARAIVKERDRKPIATTRELARVIERAVPQRGKIHPATRTFMALRIFVNEELK
                     EIEEALRKVSPLVEEGGRVVVISYHSLEDRIVKRFLKDEEEWEVLTKKPVYPTPEEVR
                     DNPRARSGRLRAGEKRSKIKEG"
     gene            complement(19744..20175)
                     /gene="mraZ"
                     /locus_tag="PKN31_01240"
     CDS             complement(19744..20175)
                     /gene="mraZ"
                     /locus_tag="PKN31_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOA98585.1"
                     /translation="MFLGHYYHTIDEKNRVVIPSSFRGEISGKVYLTQGMEKCIFLYP
                     EEVWNRFYQKITTLSLTRKETREFSRFFLANATCLDIDGQGRIVLPDYLKNYAEINRQ
                     VVWVGVGERLEIWAEEIWRPYHEEMGKKFGELTEQIVDTGI"
     gene            complement(20291..21007)
                     /locus_tag="PKN31_01245"
     CDS             complement(20291..21007)
                     /locus_tag="PKN31_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOA98586.1"
                     /translation="MAKGVFLADLHIHSVLSPCADRDMLPHCIILEAWEKNINLIAVT
                     DHNACDNVGATVTLGEKFGIWVIPGVEVETREEVHLLSFFPSLSQLHDFNQEIARFLP
                     FIPLSEELWGEEWVIDEEGEIKEKKTNLLVYPLDLSVEQVVEKVRSREGIVIPAHVDR
                     PSYSIISQLGFIPPHLEIKVVELSSNTSEEEAVNKLGLAGFRFLHSSDAHSLSQIGSV
                     TTSFLFSSFPNWRELEQILT"
     gene            complement(21000..21347)
                     /locus_tag="PKN31_01250"
     CDS             complement(21000..21347)
                     /locus_tag="PKN31_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOA98587.1"
                     /translation="MTQIVEPLKAEVVVGERMEEQDIEGGYCGDLLSDCIANAKEGSV
                     WVTIQSHPNTVAVAVLVGIPCIVVSNYQEINEETRKRAQKEGVTILRTSLTSYQAVSV
                     LFSLGIPGTKRHG"
     gene            complement(21360..22682)
                     /locus_tag="PKN31_01255"
     CDS             complement(21360..22682)
                     /locus_tag="PKN31_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOA98588.1"
                     /translation="MEEEIFHSVRLDEEKCKGCTHCIRHCPTEAIRVKEGKARIDEER
                     CIDCGECIRICPNHAKYAQSDSLEVIKKFPCAIALPAPAFYAQFKVDISLGKILQGLL
                     HLGFQEVFEVARGAEILAEEIDRYLKKREIVTPIISSACPAVVRLIEVRFPSLIENLL
                     PLDSPLGVTAKLARLQAMEKGYRPEDIGVFFITPCPAKVTESKQSEGVLGHIDGAISI
                     ANIYSRLVNIVEELEESSSLPLASFRGIGWARSGGEQESLKEGEYMAVDGIHQVISVL
                     EKIEMGELKNVVYCEAQACVGGCIGGVLAVQNPFIAKVSINHLLKKYTEFRPIRKGEM
                     EKWRREGVFERKKAYQAKEVLKLDKDFKRALEKYQMIEKLLEKLPGLDCGACGSPTCR
                     ALAEDIVRGKAEEIDCPFMLRDQLLSLSEEIHALANRAPQTMAKKKEE"
     gene            complement(22691..>22848)
                     /locus_tag="PKN31_01260"
     CDS             complement(22691..>22848)
                     /locus_tag="PKN31_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA98589.1"
                     /translation="YSTAPPHIREMGFGAGYGLSNMKKCSDRLEIDTRVGEGTKVIMD
                     FFLNSEE"
BASE COUNT         6838 a         5756 c         3734 g         6520 t
ORIGIN      
        1 atatctatcc gacaaaaagc cggctcataa acttgaattt tctctttttt catcaaacca
       61 taaacttaag aggattaaaa taattcccat cacgatgaaa acatcagcta agttaaaagt
      121 agcccagaag gagggctcta taaaatctat cacatatcca aagcgccagc ggtctaaaag
      181 attaccccag gcaccggcta aaaagagaga aattcctctg atgtaagcaa ttttaataaa
      241 aatccagaga aggacaatag tgataaaaac agtaattaag gccaatcctt ctttactcat
      301 tattttccag ccaaaggcac tgccaaagtt gtgacgtaaa gtaagcgtaa gaatcccggg
      361 gataagaaca atagaatctt cagccagatt tttaatagcc cactgtttgg tcagctgatc
      421 agcaattaag gccagagcta tagccagaga aaaccacaac catctaatgt ttccttttcc
      481 aggcttagag ttcatgattt tctcagccga ctatcctcct gaaaatcctg aaaagaaaaa
      541 cgagctattt ctcctccttc ttcttttttt tcctccttat ttccttcttc caaagcttta
      601 ccagtatccc agtctttttc cttccattct aaactatcca taaaagtctg caacaacgac
      661 ctgaaacgaa tttgaaaaag acgatattgt tccaaaaggt cctggtatcc tctttcgatc
      721 tctttttttc tctccagagc ttcttcaata attttttggg ctctaatttc tgcttctttt
      781 aaaaccattt gagattccct tttggctaat tctctctcca cctctgcttg cttttcccac
      841 tcctccatct gggcttccat ctcagccagc ttttcttccc tccccgccaa tcttctttta
      901 agctcccggt tatcttgcaa aatcttttcc atttctactg ccatgttttc tatttgagaa
      961 gccagttctt ccaaaaactc ccgtacttcg tgttcattat aaccgcgaat agagcggtta
     1021 aaatcaatct ctcgtatctc ttccggtttc caccagctca ttttttctcc tcctcatcct
     1081 ctatattctt agctcgccta taagcctcct ctacccctcc catcaccaac cccctaaaag
     1141 ctccctcttc catttttttt atcccggcaa tggtggtccc tccgggagaa gccacccaat
     1201 ttttgagctg agcagggtgc atatcccggg attttaaaag agacgctacg cccaatacag
     1261 tctgagtagc tatttttaag ctcctctccc aggggatacc tatcttcacc cctccatcgg
     1321 ctagagcttc tataaaaagg gctataaaac ctgggccact accactgaga gcggtaaaaa
     1381 ggtccaaatc ctcctcctct gcttcaaaaa cccatcccag ggaagaaaaa agaaataaaa
     1441 ggtcttcccg ttcttcggtc ttcaccatcg gagaaaaaca caccggaact acaccttctc
     1501 ccacttccac acataaattg ggcataattc gcactattgc ttgctgggga ataagctctt
     1561 ccaaatcttt gatttttact cctgctgcca ctgaaacgaa aaccgactga gaaaaattcc
     1621 ggagagaaag aagaaccttt ttcacatctt taggtttcac agccaaaaat acaaaacgag
     1681 gagcttctaa attatttaag ttctctaccc attctaaatc taaagaagaa gccagagtcc
     1741 tcgctttttc taccacctgg tccagaagga aaaaccgaaa ctttttatgc cagttatctc
     1801 gacataaacc ccggattaga gcacttccca tatttccaca accaacaaaa agtatactat
     1861 cttccatttt ctctacctct ctccaaatat agctcgacct aaacgtatca tagtggctcc
     1921 ctctaaaata gcccagggaa agtcatcact catccccata gataactcct gaaaactggt
     1981 aatccggggc atttcgcgat tcacttcttc tttaagttct cgcaaacggg agaaaatctt
     2041 ccttacttct tgttcttctc ccccaacggg accaatagtc ataagacctc tcagttctaa
     2101 atgagagaac tgagcaatat gataaactaa agatagcaat tcttcttctc tcactccccc
     2161 ctgagtctct cgaccggtaa gattaacctg aatcaaaacc ggaagttttt tattcaaatc
     2221 ccttgctctt ttttccagct cttctccttt atctatgcta tctaaagaat gaaggtactg
     2281 gaaaaattct accacttttt tcgctttatt tctctgcaat cgacccacaa agtgccactc
     2341 tacttcttca ggtaaaagag gcattttctt cagcgcttct tgaaccctgt tctctcccag
     2401 ataaatcagg cccagcgaaa tcgcatcttc aattttactc acttctaccc ctttagtaac
     2461 tgctattact tttatatcct cagggtttct tcctgctcgc tgagaaaaaa tagtaatctt
     2521 ttctttgact gcttcccaat tttcccttac cccatatcta ccacctgcca gggctttaca
     2581 tttctccagg gtgaacgcac ccattcatca tctctaccca gctccccgag taaagattgc
     2641 catgccctaa aggaccaggg gtgaggatga gacaacaatt gaggaatttt ctctcctatg
     2701 aggcgatctc cccgagcaag aagcaattga atccaggcta accgaggact ctctccagaa
     2761 aaaggaattt tcagcttacc cagacctctt tcccagattc ttttttctct ttcccaatcc
     2821 cgaatagaag gagtcctcca atcagaaaga gcagtatgag gtttaggtat taaaaaactg
     2881 taagaaacta aaacttgctg aggattcttt attcgagcaa ccaattttgt tagaaactct
     2941 aaatcttctt ctacccctcc ctccggttta cctaacataa aataaagttt tattcttttt
     3001 atcccctgtt ctaaaagttc ttttatcaaa tctatccatt cctcgttaga aagtcctttt
     3061 cccagtacca ttcgagcagg aaaacttcca ctttctggag caagagttat agtttcaact
     3121 tctccttcac aaaataactc ccccagattc tttttcctca caagagaaag agcggaaaga
     3181 gaagaaaaag tgagtttctt atttttttta aacacgtact ttattatttc ttccaaggcg
     3241 gggtgagaca ataaatccgc ccccaccaaa cctattttat cggaccaggg agaaacatgg
     3301 tcaatcattt tctctatcaa ttctaaagac ctgttgcgta aaggatggta tatatttcta
     3361 ccagcacaaa aagagcaatg atacctgcaa cccctgctta cctctacaag agaggcaccc
     3421 tgaaaatagg aaaaaggggt atagatgaaa gtacgagtct cgaattcatt aatgtctacc
     3481 cattgtcttt ttactggaaa agaaaaccct tcttctgcct cgaaaccctg aagctctccc
     3541 ccttgataaa ggggtttaac cccaccgggg atatacaccc cgggaatatt ccccagaatc
     3601 tttttctttt cttctctgct tttgcctttt tcctcccctt ccttccataa agcaagaatt
     3661 aagggaaaaa tttcttctgc ttcccctatc actataagat ccataaaggg agcaagaatt
     3721 tctggattaa gagtagcagc aggaccacca cttattaccc aggggtcttt ttcttttcta
     3781 tctcgagcaa aaagaggaat ccccccctcc tgcagtaaag agagaagatt aagaatatcc
     3841 agctcaaaag agaagttgaa tcctatcata tcaaaagaat taagaggtaa accgctttct
     3901 aaagaacggg agccatactc acaaaaaaat ctttctcctc gccaatctgg ggcttcaaaa
     3961 agaagacgga taatagactg gtaacctaag ttagtcatcc ctaaagaata atctccagga
     4021 aagcaaaggg caaacaaaaa cttacccact ttactggagg gaaaaatacc ctgctcttcc
     4081 tcccagtaat ttttggcact ttctaccatt aacttatttt tttctcagga aagcaggaac
     4141 atccagatca tcaaaggaga aggagtctgc aatctcgctc accctctctt ccttttcctc
     4201 ctcaccgtga tcaaaccctg tagcaataat ggtaatttct acctcgtctt tcattttctc
     4261 atcaactatt gctccaaaaa tgatattagc ttcttccgaa gcagattcgg tgatcacttc
     4321 tgccacttga ttcacgtccc agatactgag accaggacca ccagtaacat taaagagcac
     4381 tcccttagca ccagaaaaag gtatttccag tagaggactg cttaccgcga gatatgccgc
     4441 ttctttggct ctgttttcac caccagctct accgataccc atgagagacg tccctgctcc
     4501 ttccattata gctttgacat cggcaaagtc caggttgatt atccccggga tatttatgag
     4561 gtcagaaatg ctctttaccc cttgcaaaag aacattatcc accatgctaa aagcctctcc
     4621 tactgaagta gagggactgg caatttgcaa gagtctatca ttgggaacca caattaaact
     4681 atctactcgc tcccgcaaaa gagcaatacc ttcttctgcc tggcgcatcc tctttctacc
     4741 ctcaaaagta aaaggtttag taactaccgc tacagtcaaa atgcccagtt ctttagccag
     4801 gccagcaaca accggtgatg ctccagtgcc tgttccccca cccatgccgg cagtaacaaa
     4861 taacatatct acgtcttcca gaatttcaaa aatcttctct ttatcctctt ctgccgccct
     4921 tcggcctatc tctggattag cacccgctcc caaccctcga gttagctttg ctccaatctg
     4981 taatttgaca tcggctaaag atctttctaa aacttgagta tcagtattca tagccacaaa
     5041 gccaacccct ttaaggccag cctggatcat acgattaatt gcattacctc ccccaccacc
     5101 cacacctata acttttatgt ttacttttcc gttgcgaagc ctctgagaat tcgaacgcat
     5161 gactatcacc ctccgccctt agtccagcat aaaaaagtct ctaatccaat ctataacttt
     5221 agacccccga ttctctaagt cgagaaccag cgattttcta cctctttttg tcgcttcctc
     5281 caaaccaaat tgcagtaaac cacaagcagt agaaaacata ggatgagata cagtttggat
     5341 cattcccaca tggtctttac tttccgggga acctactctc accggtaatt ctaaaataga
     5401 agaacctaat tctgcaatac cctccaaaag ggcacttcct ccagtcaaaa ccactcctcc
     5461 ccgcaaaaga ggataaaaac ctgaagattt aatcgctcga cggatcaaca aaaatagctc
     5521 tctcactcgt gcctctatta tctcacaagc atacttcctt tttaccgtct gtagagagga
     5581 aagtcccaaa tttttagctt caataacctc atcatcagga atatgccggc tgaaagcgca
     5641 accatggtgt aatttgattc tttccgcttc gtcccgggag gttcgcagac ctacagccaa
     5701 atcagaggta atgctctccc ctcccagagg aactactcct gaataacgca tagagcctcc
     5761 gtaaaatata gctatatctg cagtccctcc tcctatatcc accagagtaa tccccatttc
     5821 tttctccatg gggtgcaaaa cagccagagc agaagccaga ggctgaaaaa ccaacctatg
     5881 aacttctaaa cccgcctgct ggaaactttt ggagaaattg tgaaattggt tctcatctac
     5941 agtaattaag tgaacctttg cccgaagaga agagccaatc atacccgctg gatcggctat
     6001 ccctgtttga tcatccacta tatattcttg aggcaagagg tggagtattc ttttcccttc
     6061 cagatttacc cctgccttag agccctccag caccttatct acatctcgag cattaatttc
     6121 tcgtcctccc ttattaagaa aaacttcatt ctccaggtta aaagaggaca cgccatcacc
     6181 ggctattccg gcatacacaa gatcgggcat cgcacgagcg atttccagag ctaaagaaaa
     6241 tgactctctg atggcccctg ctgccttatt aatatctatt attttccctc tccttacgcc
     6301 ccgagagcta ctcaatccta ctcccagaac ctcaatagca ctgctttttt tctgcccgat
     6361 cagggtgcaa attttactag ttcccacgtc caccgctccc aaaataggag agtggctatc
     6421 caaggagcgc caactcttca aaacgcatcc tcctcgctcg gcactataac cagatcatct
     6481 cctactcgaa gatcaaaccc tcctacatcc aggtacttta ccgctatatc tctcaagtag
     6541 ggcttaagaa tagccgattt tttcttcaaa ttattaaccc cttcacatct tataaatata
     6601 ccactttgta gctgtaaaat aaacagccta tcattctcca ctgtcacagc agaaagagga
     6661 agttcaaact ccttcttcca aactttaatc aagtccagtg actctaataa taaactcttt
     6721 cgattctctg gcaagtaaac ccttactaaa tcggtagatg aagcagggat ttctactcct
     6781 tcttcatcta cacaaaaaac ttcgtctccc acctctaccc aggcttctgg ttgacgttct
     6841 tttatttcta tctgcacaag atagggaaaa gatttacgca ctcttgcttc ctttacctgg
     6901 ggaagctctt ctaacctttt ttcgatttcc cagggacgga gagcaaaaat attactaccc
     6961 aaggggatat taactacctt caataattct tcttcctgta gccattgatt accacttatc
     7021 tctatccttt ttaccttaaa aattcccgtc tggaaaatta aactgaacaa ggcaaaggaa
     7081 catagtccta aaataaaata aaaaaaaaga tttgtccaaa aagcttggtt cttttctgca
     7141 ttaaggccat agttctactt ctctttctaa ccagatacct ttatttttat atacttcttt
     7201 ttgtacccag ttcataagat attctatttc ccaggatttt gctcccccca aattaacaat
     7261 aaagttagaa tgtttggagg aaatttgggc ccttcccaga cgtaagccct taaaacccat
     7321 ttcttctatt atccgagcag cactattccc ttccgggtta agaaaaacac tacccgcaga
     7381 aggccactcc aggggttgtc cttccttgcg taagtagcaa aattccctaa ttctctcctc
     7441 taaaatttct tgctcttcag gaaagagatt aaaaacagct tttaaaatca ctattttctc
     7501 tttctttaaa ttagaatatc gataagaaaa aaacaaatca tctctattta accattccct
     7561 atttccattt tcctcccgta tttctacttt ttttaccaat ggaccgattt cttttccaaa
     7621 acaaccagcg ttgatcctga cggctcctcc tactgtcccc ggcactccca ctaaaaactc
     7681 tatccctccc agacactcct cccgagcaaa gcttacaaga tgcgaaagag gagctcctgc
     7741 ccccccctct atacaatttc cttccctttt cagttcgcag aactctcctc ctaatttaat
     7801 caccactcca ggaaatccct catcttttac taaaacatta gagccccgtc ctaaaatttt
     7861 ccactttatc ccttctctct cacaggagga aatgacctca tccaaacatc ctccatttct
     7921 cacctcaacg agagcaagag cttttcctcc aactttccag gtggtaaaat tcttcatctc
     7981 cggctgagga atgatggata aatggggaat gttttctaac tgatttatta ttttcctcca
     8041 tcttgagagc aaagaatcca tcccgctttc catacatctc ctgcacccat agtaaccacc
     8101 aggtcctccg gcttcaatat ttttaaacac tctcgagctg catcttctga gctttctacc
     8161 agctgaactt tttcaaaccc atcttttttc atttcctggt aaattaattc tgcagaaaca
     8221 ccagggatgg gcttttcccc ggcagggtaa acaggcaaaa tgaagacctg ctgaaacttt
     8281 tccagaactc tggccatctc ccgatataac tgaaaagtcc gagtatagcg gtggggctgg
     8341 aatataacta tcaaattccc tccagcataa tccagagcag tttgccatac agtcaacatt
     8401 tctgtagggt gatgagcata atcatctatc accagtgccc cctttgctct ccctatcttc
     8461 tcaaaacgtc tttttactcc ccggaaagaa gaaatagcct ttttcaaatg attcagttct
     8521 attcctaatt ttagtcccac cgtaatagca gctaaggcgt tatttacatt gtgtagtccc
     8581 ggtacccgta attcaaaatc agccaatttt ttatctttcc agtccacctg gaaaatgaaa
     8641 ccatccttct ttttttcttt aatcttccct cttacatctc cttcttccat gccataggtt
     8701 aaaatttcta aagaagaaag ataaggcaat atggagcgaa ctgtagggtt gtcttgacag
     8761 accaccccga acccctcttg ttttatctgg tgtagaaagc ggatgaaagc ttttttttcc
     8821 tcttccatgt ttccataaaa aatcaagtgg tcatcttcaa tattggtaac tacagcacag
     8881 aaggaattaa gtttcaaaaa agagccatca ctttcatctg cttcagctat gaaaaattct
     8941 cccctgcctg ctttcgcatt ccctccgata tcctccagtt cccctcctat agaaacagta
     9001 ggatccaggc ctgccatttc aaaaagaaga gaaatcatag aagtagtagt agttttacca
     9061 tgggttccgg ctaccacaat gccttttttg ctgtttacca gagatgctaa catctctccc
     9121 cggggaatga tgggaagatt taatcttcta gcttctttta attcttcatt ttcttcagga
     9181 atggctgaag aaacgataac cgcttctgca ccccggactt gttccagacg gtgacccaga
     9241 aaaactcgta ttccttttcc cttcaaacgg gctatagctt cattctccgt aatatcagaa
     9301 ccactaacca cgtatccttg ttcatgagct atcagcgcta agctactcat ccccgctcct
     9361 ccaataccaa taaagtggag gttacggggt aagagataat gtgtcttgtc caatcccttt
     9421 cccccttttt agttcctcga ttatggtctg tgcaatctgg gaagccgact ctctcagata
     9481 ttttcccctt aaatcagaaa aaacacaaga ggaagaaatc aaaatctttt ccacctcttc
     9541 tataagcaag gaagaggtta aatttttttc ttctataact cgagctaatc cttgcttctc
     9601 taaccactga gcattataca gttgatggtc atcggttgca tctccataag gaatgagcac
     9661 agatggcaac tcaaaccaga aaagttcaaa aatagtgtta gctcccgccc gggcaatagc
     9721 cagatctgcc gcagcatagg ctatccccgg ttgaggcaaa aatttgtaaa ctaaataagg
     9781 agaggagaat ttctctgcca tttctttggt gtgctcaaaa ccctcttcac cggtgatatg
     9841 gattacctgt aatccttttt tttctaacca atccatggtt cctaaaaaag tggtgttaat
     9901 tacctctgag ccccggctcc cccccataac cagaatggtt tgtctatcct tcttcagtcc
     9961 taacttttgc cgggcctcct ctttttttcc cttccaatcc attacttctt ctcgcagggg
    10021 attgcccacc acgtcgatct tttcctccct atctttccag taaacaatag cctctggagc
    10081 agaaatgaaa attttcctgg cccagcgaga tactaatcgg ttggataacc cgggataaat
    10141 attttgttcg tgaatataaa tgggaacacc taaaaccctc gcccatatag cactcaaaag
    10201 agaaaggtag cttcccatgc ccaatagagc tcgagggcga taacggaaaa aataaaaagc
    10261 agtctggaaa aatcctatta aattatccat caacacagaa aaaaaagaaa aatcccattt
    10321 tctattccaa cctcgagccc ggacaaaaag aagaggaaag tctgcttctt tcaacaaatt
    10381 ttcttccatt cctcgtttgg ttcccaaaaa tattacttga aagcctctct ttcttatctc
    10441 ttgagcaata caaagagaag gaaaaatgtg ccctcctgtt cctcctgcag caataaaaat
    10501 cgtatccatc tattttgtac ctcgcgcgac attaaacaaa atccccagct cgctaagagt
    10561 gattattaaa gaagaactac catagctcaa taaagggagc gtcactccgg taacaggaag
    10621 aagcttcaaa accgcggcca tgttaatgaa agcctgcaat ataatactaa gagtaacacc
    10681 catagccacg agtcctctga attcatcttg agatccttga gaaattttcc aaccccgcca
    10741 caggataatg ataaacagta tcaccaccgc cgaggttcct ataaatccta attcctctcc
    10801 tataatagca aaaatgaaat cagtatggcg atcgggaagg aaaaaaaact tttgacgact
    10861 ttcacctaac cctcgtccca ctattccgcc tgatcccaga gctatgagag attgaatgat
    10921 gtgaaaccct ctgcctaagg gatctttcca tggatcaaca aaagcataga tacgctctcg
    10981 ccagtaatcc ttacctccaa aaagaatgag aaaaacacca ctgggtatta aaactatcaa
    11041 aatcaaaagg aggtgctgca atttactccc tcctaaaaac aacataagaa aggcaacaag
    11101 aaatagaaaa aacgcggtac tcagatctgg ctcgatcaac actaaaagac aaattaaccc
    11161 cactaaaatc ataaaaggca taattccttg ccaaaaatct tccactcttt ctttgtaatt
    11221 cgccagagca tcggctaaat ataaaattat actcaattta gctaattccg agggttgaaa
    11281 gctcaaagcc cctaaattta tccaccggta agagcctccc cttactctcc ctacaccagg
    11341 tacaaaaacc aaaatcaaag aaacaaaagt agccaagagc aaaaagcggt ttatactccg
    11401 caagcgatct aaagaaacaa aagtaaatat agttaaaaaa agaaacccca gtaaaattcc
    11461 taaaagatgc cttttcaaaa agtaaaaaac atcgccataa gcgtacaaag cagtggtcat
    11521 gcttgcacta aataccatta caatccctaa aagcaatagt aaaagtattg accaaaaaag
    11581 gtaggggtca atatcccacc accacctggt tttaaaagaa agcctctcgt cgttaacagg
    11641 aagtgttatt ttgttccaag tcataaacca attgacggaa gtgctccccc ctttcctcaa
    11701 aattacgata ctgatcccag ctagtgcagg ctggagagag aagaatagca tccccctcct
    11761 gagctaggct tcttgctctc ttcactgctt cttcaagaga agaaactaaa taccgttcaa
    11821 gatgagaggg gataaacctc tctatcagat tccgagattc tccgtaaata accgctgctt
    11881 taactttact actttgaaga gcagagttaa gttcactaaa gtcatcaatt ttcgctctac
    11941 ctcccaggat taaaacaaca ggacaagata aagactctac agctactctc gtcgaagaag
    12001 gagtggtaga ttttgagtca ttaaaataat ctatccccct ccagcttcct actttttcta
    12061 aacgatgagg aagagaagaa agaaaagata ttgcttcttc tactatctta tcctctatcc
    12121 ctaagagtaa agctatagat atggcagcga gtaagttttc ttgttgatgt ttcccgggaa
    12181 gagaaaagtg agaaagagga aaaagagctt tttcaccttg aaaaacttcg tttccagaaa
    12241 tataaaatcc ctcggtgagt tttttatctc gagcaaccgg caccaattga gatttaacta
    12301 atcgggaaaa acgagttgac caatcactga gagaaaaatt gatgatagca aaatcatccc
    12361 ttctctgatt ctcaaaaatg cgagccttag cttgaaagta attttctata gtagaatgat
    12421 aatccaagtg attaggaaac aaattcagca ctacagctac cgaaaaatga gcctttttta
    12481 tgcgagacag ctgaaaactg cttaactcta ctattcccca atcaaatttt tcacgagcag
    12541 aaagactttc tatcaaagga taaccccagt tacctcccac aaaaactgac cagccagccc
    12601 tctccaaaat ttgtttggtc caaaaaacaa cagtactttt tccacaagaa ccggtaattc
    12661 caatgagagg aaaggaaata aaccgagtgg ctaactctat ttcgctgatc accggtattc
    12721 tgcgttcttc taaagcccgt aaagccggat gagtcggtgg tataccagga ctggtgatga
    12781 cctcagaaat agaagaaaaa tcagtctgca aaaaatcgga ggaggaaagg aactcaaacc
    12841 tctcactttc tgctatatct cgaggtattt gctcctcttt aagataatca tctatagcta
    12901 aaactttttc cttttgttca aggagaaatc ggctgactgc tattcctgtt ttacccaaac
    12961 ccagaactac ctgcattttt tcacctcatt atccctaaaa gtgcggccac tactcccacc
    13021 ccctggatga tccagaacct tacagtaatt ttactctctt tttctcctcg gagttcaaaa
    13081 tgatggtgga gaggactcat aagaaatatt cttttcccaa atactttgaa ggaaaacact
    13141 tgaagaatca ccgaaagggt atccagaaga taaacaaaac cggctaaagc taatagccag
    13201 gaatagccag aaaaaagagc taaaatcccc ataagagctc ctaaagacag agagccgcaa
    13261 tctcccataa aaatccgggc gggccagaaa ttaaaccaga gaaaagaaag cagcgctccg
    13321 ctgaagataa ttgctacctg agctaaagga aaatttccct ctaaaaaaaa gagccccgcc
    13381 caaaaactaa aagtcaaaat tccacaaccc gctgccaacc catccaaacc atcggttata
    13441 ttaaaagcat tagtagaggc caaaattaaa aacaaaccat aaagaaaaaa aggtatttcc
    13501 cccaactcta tgattccact tccatagggg acaattaaat agtgagggaa aagaggaaat
    13561 cctaaaatta aaataaaaac agaaaatacc agttccagaa gaaatttgtg gcgggctttc
    13621 aagctccagg gttctccgcg aaaactttta aaataatcat ctaataagcc taaaccagca
    13681 aaagagagca aagttataag tataatccag atctgggggg taaactgctg gaagaaagag
    13741 agcaccaata atccagagag aatcataatt attcctccca tactgggagt attttcttta
    13801 tgaagatgga gattggggcc ttctcttttt attttctggc ctatacgggt tttttcttgc
    13861 cagcgaatga aaaaaggaaa gaaaaacaac cccagagcaa aaaccataag aagagaaaaa
    13921 atcagacttt taatctcgtt catggttttc tctccactct ggaggaatag ccttctccag
    13981 ctctaacttc ctcgatccct taaaaagtat tgcccaccgc tctcccctgg gcgtatagcg
    14041 agagagaaat tctcgcacct ccagagaggt agaaaatagt aaaaaccggc cctcctgtat
    14101 ttcctcttta gctatttctt ctgccgctct tttcatttct tctcccacca atagagtacg
    14161 agaaagagaa gattttttta gctccctcaa aaactctcga tgagcttcag cagaaacacc
    14221 ccctaattct aacatatctc cccatactcc ccaggtttca aaaccatggc aagctaaaaa
    14281 atccagaaac tgcaaagatt tacgagaaga aagaggatta gcattataag aatcatcgat
    14341 gacgattcct ccctggtaga agaaaaaatt acctcttccc tgaggaggct gccaatctct
    14401 taaacttcct tctatttcct ccactcctat acccagttta caagccagat ggaaagcagg
    14461 caatagcata actgccactt caggaaaaag aaaaggagca aaaaaacaga agtcattttc
    14521 tcccattttt atctctattt tagctttcca ggttaaagga tcgagcgaga aacttttcaa
    14581 gtaaatgtga ttggcagggg aaaagccaaa agatataatt tttttcccct ctatcttttc
    14641 ccctgccaat ccactccatt tctggtcggt attgagataa attatctcgg tcttttccct
    14701 gaccagttta attttttctt ctactacttc ttccacacta cctaaaccct ccagatgccc
    14761 ttctccaaaa gaagtaaaaa ccaccatgtg agggtcaata atttctgcta attcttccat
    14821 ctcttttttt ttgcttatcc ccgcttctat tattaaataa tcattatttt ctttaaaatt
    14881 ggctatagag ctcgctactc ctattactgt gttaaaactc tgaggagttg cctctaccag
    14941 aaaacgcctg gatagtaaag aggcaaaaaa gctcttgcaa gtagttttgc ccaccgtgcc
    15001 ggttatggca attttatatc ccgataattt ttgagaagcc ccccttccga gatgctggag
    15061 agctaaaaaa gtatccggta ctcgaaaaac ataagtttct tcttctccat cttcaaaacg
    15121 actgaccagg actcccggcg cacctttctt tatcgcttct ttcacaaata aatgtccatc
    15181 agtcctttcg cctttaagcg ctacgaaaag ttgccctgct ttcagggaac ggctgtcaat
    15241 ggccaccccc tcaaaaaagg gagccttccc tgttttctgc atttcagctt gagtaatttc
    15301 tactatttct tgaggataaa acttcatttt tcttttcttt caatattttc caggctatat
    15361 agtggtcgtt gtgaggaatc gctttcccgc catacacctg gaatctctcc gggcccttcc
    15421 ccgccaaaaa aactgtatct cctttttgag ccaattccag agcctgccgg atggcatctt
    15481 ctcgatccag aattacctca aaatgtagat ccttattctt tgctgctctt tttactcctt
    15541 ctaaagcatc aaaagcagtt tgctccggac tctctccccg gggattatca gtggtaacaa
    15601 tacatgcatc actgaagcga gccaccgttt ctccgatgaa aggccttttg ctcctgtctc
    15661 tttctcctcc acagccaaat acggtaatca ctttccccgg agttagatct cgaataatag
    15721 agagactttt ttctaaccca tgccagttat gagcaaaatc tactatgacg gtaaaatctt
    15781 gacctgcttc tacaagctcc catcgaccag gaatatgatg aacctggaat agcgcttctt
    15841 gaatatcatt tatatctaaa ccataaaaaa gagccaaaga aatggcagaa agagcattgt
    15901 acacattatg aatgccagga aaatgaatta ccacttccat tttcccccag ggacttttta
    15961 ctaaaaaaga gctttgctga agagaacaag taatgttctc tcctcttatc tctgcctcct
    16021 tgtttagacc gtaaatgaga taaggaactc ctattccttt tctcatttta aggcaatttt
    16081 cgtcgtcccc attcaccact actacccgag gataactctt agagagattt tcttccacca
    16141 tctgtagaag ttttctcttg ctctcaaaat aatgttcgaa agtacgatga aactccatat
    16201 gttcaggaat taaattggta actaaagcag aatcaaattt tattccttcc actcttctta
    16261 aagctaaagc gtgagaagaa acttcgacta ctgcgttttt tatacccatt ttttgcatct
    16321 cataaagatg gcgataaaga gaaagagact cctcggtggt gtttatggct tcttcttctt
    16381 taggacctat tatgttgcgg gctgtggtaa gaagaccaca gggaaaacca cttttttccc
    16441 aaatggcgcg gaggaaaaaa caagtagtag tctttccatt agtgcctgtt actcccataa
    16501 ccctgagaga agaagaaggg tcaccataaa aaaaggagga ggccagagcc agagcttttc
    16561 tcccttcagg aactaaaacc caggatacct taacttgggg aggaagagca aattcttttt
    16621 tttctaaaac tactaaagtt gctccccttt ttatcgcttc ttcaatataa aagtgaccat
    16681 cggtattaga accaggaagg gcaaaaaaaa catagcccgg ctgtacccga cgagaatcaa
    16741 aaacaaggcc tctaacttct tcctcggggt tttcaatttt tccttcttca aaagaaaatg
    16801 aagacaaaaa ctgacctata gtcaaaatag ccctacctcc gcttcatctg acattatacc
    16861 agcatagtta ataatgttct gagcaatttc tctaaaaaca ggagcagcaa tatctccgcc
    16921 ataatattct ccttgtggtt catccagaac tataataatt ccaaagcgag gctcgggaag
    16981 agggaaaaac cctacaaaag aagaataaaa cttgcgaggg tcatagcggc cgtcaagtcc
    17041 cactttctga ccagttcctg ttttcccccc tatacgatat ccttctatat aagccttttt
    17101 tcccgttccc tcttttactg ttttttccat catttccaga actaaagatg ctgtccgctc
    17161 cgatactacc tctcctgaag aaacaggaga aaatccctct attatctctc ccgtggggct
    17221 gattattttg ctcaccaagc gaggttgcat caccaccccc ttattagcca gagcagaaag
    17281 agctctcaaa agctgcaagg gagtaaccat catctcctga ccaatgggaa tagcacctat
    17341 ggagagtaaa gaccaatctc gggaagggcg taaaagaccc tgctcttctc ccggtaatcc
    17401 tatatcgcat ttttctccta atccaaaatt cttcaaataa taaaatagtt tatccttgcc
    17461 cagcctttca gccagagtaa taatccccac attgcaagaa ttaattaaca aatcttccag
    17521 cttctgttcg ccatgagctt taatatctcg cacccgatga ttatacacca taacaaaacc
    17581 agggcaaaag aatctctctt caagagaagc ttttttttct tctaaagctg cagccatcac
    17641 caatggtttt accgtagaac ctggctcgaa aaccatactg atagccaaat ttttccagga
    17701 ttcagaggga aaatcttgaa agttgcgatt atcaaaagaa ggacggctga ccatagctaa
    17761 aatgtctcca ctctgagggt tattaactat taccactcct cgaagagctt tagttttttc
    17821 catagcctga tctaatgctt cttcagcaaa aaactggatg gtgctatcta cagttaaaag
    17881 gagatcgtta ccactttgag gtgataagtg tttagtagta agaggtattt ctcgccccag
    17941 agcatctttt tcgtaaaatg tatagcccgg tttacctgca atataagaat caaaggcaaa
    18001 ctctaaaccc tccagcccct gtctatcttt tcccacaaaa cccagaagat tactaaataa
    18061 aggggcttga gggtaaaaac gaaccggttt ttccttccag cttatccctt ttagcttttt
    18121 ctcctccaga gcttccttca aatagagggg cgcattgtcg gtaatctcta tccagaaaag
    18181 cccgctctgg aatttttgct gcaattcaga cgctgaaagg gaaagaagtt gagataattc
    18241 ttctgctatt ttctccgggt cgtccatctg aggagggtaa gcgctgatag aaagaacccc
    18301 cacatcctca gccagtactt taccgcttcg atccagaatt gaccctcgag agggcaaagt
    18361 ttcttctaat actaaacgag ataaggcttc ttctcgataa aatttgccct gtaatacctg
    18421 aagggaaaaa acgcgaactg caacaactcc taaaaggaat aaacttaaaa aaaacagaaa
    18481 atttgcccgt cggacaaatt ttctggaaat cattctcctt gtgtctcctc aggcatttta
    18541 cccaggacca gctctccttc tccgcccccc aaagccgagc tggacaccat atagacagtt
    18601 tgatcgggat aggtcatacc cagctgattt attgccactt cttttattct ctccagcgaa
    18661 gataattggg caatttccat ttttaatttc tccctggttg ccaacaaatt atcaatattc
    18721 tccctttttt gttccaccaa atagcttttt tctaaaagca aagcttgcaa ggtaacacag
    18781 aggaaagaaa gagaaaaaat aaaaaataga ccaaatatca gccaagattt aacccgcttc
    18841 attcttatcc ctccttaatt ttacttcgct tttctcctgc tctcagacga ccactgcgag
    18901 cccgaggatt atctctcacc tcttcaggag tgggataaac aggttttttg gttaatacct
    18961 cccactcttc ttcatctttt aaaaatcttt ttactatccg atcttccagg gaatggtagc
    19021 taattaccac tactcttcct ccctcttcta ccagaggaga aacttttctc agagcttctt
    19081 ctatctcctt taattcttcg tttacaaaaa tacgcaaagc cataaaagtt cgagtagcag
    19141 gatgaatttt tcctcgttga gggactgctc tttctataac ccgggctaat tcccgagtag
    19201 tggctatggg ttttctatct cgctccttca ctatagcccg agcaattctt cgagaaaacc
    19261 tttcttctcc gtaataataa atcaaatcag ctaattcttt ctccggaaat tcattgacca
    19321 cctgataggc atttcgttcc tcagataaat ccattctcat atccaaaaat ccttccctct
    19381 ggaagctaaa acctctctct gctttatcca gctgccacga ggataccccc agatcaaaca
    19441 atatccccga aattttttct atacccagag aaaaaagaac ctggtcaatt agagaaaacc
    19501 tttctttgac caaatatact ggaagggaag ggtttttcag ggtttccttg gccattttta
    19561 tggcttcttc atctcggtct atccctacca atatagcccc ttcatcgagc tcacccagaa
    19621 tggatagagc gtggcctcct cctcctactg tagcatctac ataaattccg tcttttctcc
    19681 agagaagata atggagcact tctttctcca ttaccggttg atgaaaaaca taagtgttca
    19741 tatctaaata cccgtatcca ctatctgctc agtaagctcg ccaaatttct ttcccatctc
    19801 ctcatgataa ggtctccaga tttcttctgc ccagatttcc aggcgttccc ccacccccac
    19861 ccaaaccacc tgtcggttta tttcagcata attttttaag taatcaggaa gaacaattct
    19921 tccttgtcca tcaatatcga ggcaggtagc attagccaga aagaagcggg aaaattctcg
    19981 agtttctttt cgagtgagag aaagagtggt aatcttttga taaaatctat tccacacttc
    20041 ttcgggatag agaaaaatac atttttccat tccttgagtg agatagacct tcccggaaat
    20101 ttctccccta aaggaactgg gaatcaccac tcgattcttc tcatctatag tgtggtaata
    20161 atggcctaaa aacatttccc acttcctccc actttttacc attatacccc acttagcata
    20221 aactaataaa gataaaagat aaatagaacg ttcgttccaa aaaagggaaa ttttctatta
    20281 gaggaaaatt tcaggtgagg atttgttcca gttctcgcca gttgggaaaa gaagagaata
    20341 aaaaagaagt ggtaactgag ccgatttgag aaagggaatg ggcatcagaa gagtgaagga
    20401 atcgaaagcc tgctaaacct aatttattta ctgcttcctc ttcagaggta ttactcgata
    20461 actctaccac ttttatttcc agatggggag ggataaagcc aagctgagaa atgatgctat
    20521 aactgggacg gtctacatga gcggggataa ctattccctc ccgacttctg actttttcca
    20581 ccacttgctc taccgataaa tctaaggggt aaactaaaag gttcgtcttt ttttctttta
    20641 tctccccctc ctcatctatt acccactctt ctccccacag ttcttctgac agaggaataa
    20701 aaggaagaaa tcttgctatt tcttgattaa aatcgtgaag ttgagataaa gaagggaaaa
    20761 aagaaagaag atgcacctct tctctggttt ccacctccac tcctggtatc acccagatac
    20821 caaatttttc tcccagtgtc acagttgctc ctacattatc acaggcattg tgatcggtta
    20881 cggcaatcaa attaatattt ttctcccagg cttccagaat aatacaatgg ggcaacatat
    20941 cccggtcagc acaaggagac aaaacactgt gaatgtggag gtcggctaaa aaaaccccct
    21001 tagccatgtc ttttggtgcc tggtatcccc agagagaaaa gaacactaac cgcctgatag
    21061 ctggtcaaag aagtgcgtaa aatagttacg ccttcttttt gagctctttt ccttgtttct
    21121 tcgtttatct cctggtaatt agaaaccact atacaaggta tgcctactaa cactgctacc
    21181 gccactgtgt ttgggtgaga ttggatggta acccagacgc ttccctcttt agcattggca
    21241 atgcaatcac tgagaaggtc tccacagtat cctccttcta tatcttgttc ctccatcctc
    21301 tctcctacta ctacttccgc ctttaatggc tcaacgattt gagtcactct caaaattttt
    21361 cattcctcct tttttttagc catggtttgc ggagccctgt tggctaaggc atgaatctcc
    21421 tcggataagc tgagcaactg atctcgtaac ataaaaggac aatctatttc ttccgccttt
    21481 ccccttacta tatcttctgc cagagctcta caggtgggag aaccacaggc cccgcaatcc
    21541 aaaccgggga gtttttctaa aagtttttca atcatttggt atttttccag agctcgctta
    21601 aaatctttat ccagttttaa aacttcctta gcctggtaag cctttttcct ttcaaatact
    21661 ccctctctgc gccatttctc catttctccc tttcttatgg gtcgaaattc agtatacttc
    21721 ttaagtagat gattgatact taccttagca atgaaaggat tctgcactgc cagtactcct
    21781 ccaatacacc ctcccacaca ggcttgagct tcacagtaaa ctacattctt caattctccc
    21841 atctcgattt tctccagcac tgaaataact tgatggatgc catccacagc catatattct
    21901 ccctctttta atgactcctg ctctcctcct gagcgagccc agccaatgcc tcggaaactg
    21961 gcgagaggta aagaagaaga ttcttctaac tcctctacta tattaacgag acgagaataa
    22021 atgttagcta tagagatagc tccatctatg tgtcctaaaa caccttcgct ttgtttagat
    22081 tcagttacct tggccggaca aggagtgata aaaaaaactc cgatgtcttc gggacgatac
    22141 cccttttcca tagcctgtaa tctcgccaat ttggccgtta ctcccagagg agaatctaag
    22201 ggaagcaaat tttctattaa ggaaggaaat ctgacctcta tcaaccttac taccgcagga
    22261 catgcagaag aaattatcgg agtaactatc tctctttttt ttaaataacg atcaatctcc
    22321 tctgccagaa tttctgcccc ccgggctact tcaaaaacct cctgaaaacc cagatgtaaa
    22381 agaccctgga gtattttacc cagagaaatg tctactttaa attgagcata aaaagcggga
    22441 gcgggaagag ctatagcaca ggggaatttt tttataactt ctaaagaatc agattgagcg
    22501 tatttagcat ggttagggca aatccgaatg cattctccac aatcaataca tctctcttca
    22561 tcaattcgag ctttcccttc tttcacccga atggcctccg tgggacaatg gcgaatacaa
    22621 tgggtacagc ctttacattt ctcttcatct aggcgaacag aatggaaaat ttcctcctct
    22681 atcacagaac ttactcctct gagttaagaa aaaaatccat aatcacttta gttccctctc
    22741 ctacccgggt atcaatttcc aggcgatcag agcatttctt catattagaa agcccgtatc
    22801 ccgctccaaa acccatttct cttatatgag gaggagcggt agaataac
//
LOCUS       DAOPSS010000009        22016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_1982_length_22016_cov_8.487273, whole genome shotgun sequence.
ACCESSION   DAOPSS010000009 DAOPSS010000000
VERSION     DAOPSS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 22016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22016
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1982_length_22016_cov_8.487273"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKN31_01265"
     CDS             <1..795
                     /locus_tag="PKN31_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA98590.1"
                     /translation="DVFGVVWDRSIDKDIGNVQKPVLSKPSLQGYRFPNPLDHRFFQD
                     IPEKIQRYPDRFRVFQIGFSLYERAWTLRGMEQLMMDFYDHPDFVHELLHTIADYNIA
                     QVKEAMKYDIDAVYFGDDWGQQRGLQMGPKIWKEFIYPELRRMYGSAKQEGKYVFIHS
                     CGDVDELFDDLIEIGVDCFNPFQPEVMDIFQLIEQYRDRLSFHGGLSTQRTLPYGTPQ
                     DVVRETRRLIDMGRPGGYIFGPAHDVEGDVPLPNMLAFIEEAQKQV"
     gene            1298..2395
                     /gene="ddlA"
                     /locus_tag="PKN31_01270"
     CDS             1298..2395
                     /gene="ddlA"
                     /locus_tag="PKN31_01270"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOA98591.1"
                     /translation="MKRKLRVAVICGGKSVEHEISLQSAKNVIEAMDREKYDIVLIGI
                     DKDGRWHLKALADFEEEMEGSLLPHLTSRGDEVIFIPGGAGQLVNLSTGERLQPVDVV
                     FPVLHGPFGEDGAIQGLLKIAEVPFVGAGVLGSAIAMDKEVSKRLLKEAGIPAAKFLS
                     FAREEKEEIKFPEMVSQLGLPFFVKPATLGSSVGISKVKKEEEFFSALEEAFRYDGKI
                     LIEEYVEGREIECAVLGNEKPRASCPGEIIPGHEFYSYEAKYIDPEGALLQIPAPLPE
                     NIAEKIQCLAIQAFRALSCEGMARVDFFLRGEEIIVNELNTIPGFTSISMYSKLWEAS
                     GIPYPQLIDELIQLALERSEKEKSLKTFINW"
     gene            2466..2978
                     /locus_tag="PKN31_01275"
     CDS             2466..2978
                     /locus_tag="PKN31_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOA98592.1"
                     /translation="MRKTWLFVALVSVLVLSIAFPIFAQERSSPPREERDREMRQDRL
                     HQNINYSLNLSQEQKIKILEVWEKYRPNIRDLQDQMEEARISLWKLSFETPSEEVASE
                     IRTKIEEINSLEQKIQESRNAMFQEVLSILTPEQTSQLAKQYPQRRGKGFTFQRDTGF
                     PGMGFPANIR"
     gene            3149..3829
                     /locus_tag="PKN31_01280"
     CDS             3149..3829
                     /locus_tag="PKN31_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA98593.1"
                     /translation="MAIILVVEDEESVRELLRLYLEKEGFQVELASDGEEALKKMSRI
                     SPDLVLLDLMLPKKDGWEVCREIRKTSYVPIIMLTARGEEFDKVLGLELGADDYVAKP
                     FSPRELVARIKAVLRRFNASSGGGKTWEALGLRIDYEAKKVEIKGKEINLTPKEFELL
                     WFLVNHPQRVYTREQLLEYVWGYDFAGDARTVDTHIKRLREKLSEGGISSSIKTVWGY
                     GYKFEPER"
     gene            3826..5619
                     /locus_tag="PKN31_01285"
     CDS             3826..5619
                     /locus_tag="PKN31_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA98594.1"
                     /translation="MKKTLFSKLLLSYLAVVALSFLVSGILSSQFFSRHYLASQEKEI
                     AEQAKTISEFISQSPREEIPEILPRLRKGTQILLLSRQELSSPNFPLSEPPRPPWGRM
                     GREPLPREMLEKVFQGETVVFRGFSPRFDQAMLIAASPVLQNGEVSSVLLLLSPIVNI
                     QATVSAVRRIILIASAISLVFSLILSYFFSRSLTRPLQKISEAARDLASGNLQRRIEV
                     HTEDELGQLAQDFNYLSSALEETINHLHQEKRETENILLHMAEGVVAIDEEEKITSIN
                     PALLKKLKWEGEAPLGLHVAQLFTQVQIQEIFSRALKEGKEEAQEFQVENGDHFVVHI
                     TPLKEGEKVYGAVGVFQDITELRQLEELRRDFLANVSHELRTPLTSIQGFVEAMMDGV
                     IGDEELKKKYLEVIHRETLRLSRLIHDLLDLSLMESKKIEWEVNPLSLPQMIDQVLLK
                     LMPQIEDKKIQIKKDFPSQLPLVLGNMDRIEQVLINLLSNALLFSPPESTVEIKVWEE
                     EKEVAVSIKDQGEGIPEKDIPHLFERFYRVEKSRSREKGGTGLGLAIAKQIIENHGGT
                     IKVESQLQEGTVFTFTLPIASESGGDDTIDS"
     gene            complement(5841..6062)
                     /locus_tag="PKN31_01290"
     CDS             complement(5841..6062)
                     /locus_tag="PKN31_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOA98595.1"
                     /translation="MACVTPEGKPTESGKKILNALKSGAASPEEIAKTSGLPLFRVRS
                     GLRDMVQADLVKEEEGKYKLTEKGEQFIE"
     gene            complement(6078..6728)
                     /locus_tag="PKN31_01295"
     CDS             complement(6078..6728)
                     /locus_tag="PKN31_01295"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOA98596.1"
                     /translation="MEELREEIISFPRVGEMAPSFEAVTTHGTLRLEDFKGSWLVLFS
                     HPADFTPVCTTEFIAFSQAYPEFQKRGAELLGLSVDSVSSHIAWARNIQEKTGVKVPF
                     PIIADLSKEVSIKYGMIHPGESKTETIRCVFIIDPDQVIRAILYYPLTTGRNISEILR
                     IIDALQTTDKYGVATPANWQPGEKVIVPAPTTVEGAEERLKEGYECVDWYLCKKSV"
     gene            complement(6732..7187)
                     /locus_tag="PKN31_01300"
     CDS             complement(6732..7187)
                     /locus_tag="PKN31_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOA98597.1"
                     /translation="MRTIIIMEEMNKIKKTKQREAILEILKNTKSHPTADEVYQKVRQ
                     KIPRISKGTVYRNLKLLTENKEVMKIDTGGTEGRYDARVENHYHFRCESCGKVFDVDV
                     PVDTELDKKVSQKTGFNITHHVLEFRGFCSDCQKKAIISPSGDKNKEEV"
     gene            8097..11828
                     /gene="rpoB"
                     /locus_tag="PKN31_01305"
     CDS             8097..11828
                     /gene="rpoB"
                     /locus_tag="PKN31_01305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOA98598.1"
                     /translation="MSVSYIERQSFAKIPSSLELPDFIEIQKKSFDWFLQGDVPPENR
                     KKQGLQEVFLEVFPVQDFTGRMVLEFVNYRLEEPVFTVKEAKEKGRTYSVPLHARIRL
                     IDRQTNEIKEQDVYMGDIPLMTEWGTFVVNGAERVVVTQLVRSPGVFFSREVTTTGKV
                     DYGFKIIPNRGAWLEFGIDTGDIIYVRVDKRRKINAVTLVRALGYEDNEEILSMFDYH
                     PIIRKTLEKDPSTNYKEAVVEIFRRLRPSEPPTEENTRDFMRYLFFDPRRYDLGEVGR
                     YKINNKLKLEERVIRIEEPVLAEDIVIDLQDKKCLRDISLLGEDIDTSRYRIAGKKGE
                     RINRRLAQELEKLNEEYPIEYIKVFSEEELPVKIYVEREDTWEVLSEDLEGRILSEAV
                     VDEITGEVLVEKGKPLTLGLIRDLRGLGIERVRVKKYRTLTPEDVVGSIRYFLNLIDG
                     IGYEDDIDHLGNRRARMVGELLQNQFRIGLLRVEKVIRERMTIQPDVESATPQSLINV
                     RPIVAAMREFLGSSPLSQFMDQVNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHYT
                     HYGRICPIETPEGPNIGLITSLCMFAKVNDYGFIETPYRKVVDGRVTDEIVYLSADEE
                     DLYHTAPPDVPLDEEGYIQTENTIARFREKIVEVPRDKVDFMDVSPQQIISASAALIP
                     FLEHNDANRALMGTNMQRQAVPLLVSHPPYIGTGIEYKVAQDSGATVIAKRGGVVKKA
                     DALQIEIEANDGGIDVYELNKFQRSNQSTCINHKPIVRKGDKVEKGQIIADGPCTSMG
                     ELSLGKNVLVAFMPWEGENFEDAIMISERLVKEDVFTSIHIDEYEVEARDTKLGPEEI
                     TRDIPNLGEDMLRNLDEEGIAIIGSDVKPGDILVGKVTPKGETELTPEEKLLRAIFGE
                     KAREVRDTSLRIPHGEYGKVIDIKVFSRENGDELAPGVNKLVKVFVAQKRKISVGDKM
                     AGRHGNKGVVARIVREEDMPYLPDGTPVDIVLNPLGVPSRMNVGQVLETHLGWIANKE
                     RQFLSSPPFDGAWEEEIMELMRKVEAPEEQGLFDHFFDSRISSDFKLLPQGKTILYDG
                     RTGEPFDNEITVGYIYMLKLAHLVETKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEM
                     EVWALEGYGAAYTLQEMLTVKSDDIAGRVQAYEAIVKGKNILKPSLPESFKVLVKELQ
                     SLCLDVRVFDSKKREISLEEVEEEEDTPILDINLQRREK"
     gene            11847..16802
                     /gene="rpoC"
                     /locus_tag="PKN31_01310"
     CDS             11847..16802
                     /gene="rpoC"
                     /locus_tag="PKN31_01310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOA98599.1"
                     /translation="MIDVNDLGAIQIKLASPEEIRKWSHGEVKKPETINYRTLKPERD
                     GLFCEKIFGPTRDWECYCGKYKRVRDRGVVCDRCGVEITRSSVRRERLGHIELAAPVS
                     HIWYFKSIPSKMALILGILPKNLEKVLYFASGRKREDCYKVVEPGSTDLEAGSTIRDI
                     EYKIYSNYDPNFKAEPAYRIAEVYSLPFTIGDRLSVKEYTRYRSKYKESFTVEEVEED
                     QFEVVDIRVFPYQREDELSESEVKELQEIFGGLFEETPLSETVEEPCYIVIDPGQTGL
                     KVAQIILETEARLFTHYDSTFKAGVGAEAVREILSNIDVNQLARELRKELKEASGQKA
                     KKLIKRLQVVESFAKSGNRPEWMILETIPVLPPDLRPMVQLDGGRFATSDLNDLYRRV
                     INRNNRLKRLLQLGAPEIIVKNEKRMLQEAVDALFDNGRRGRPVLGPGNRPLKSLSDM
                     LKGKKGRFRQNLLGKRVDYSGRSVIVSGPRLAFDQCGIPKKMALELFKPFVMKKLVDR
                     GFAQNIKSAKRMVEKARDEEVWAALEEVIAEHPVLLNRAPTLHRLSIQAFQPVLVEGN
                     AIQLHPLACSAFNADFDGDQMAVHVPLSAEAQAESQIIMLSSHNVLSPASGKPVAVPT
                     QDMVLGSYYLTLWGGGESRRYFVDVDEALITYNLGNLDLHEPIRIREISEKYPEIEET
                     TLGRAIFNELLPDAMPYYNEPVTKKKLSEIADLCYRKLGNEATVTVLDKIKKAGFHFA
                     TRSGTTISVQDLEIPPEKWSIIEEANRRVEEINQHYAEGLITEEEREQKVIDVWTNAS
                     NKVADAILEELDEFNPVNMMAKSGARGSIRQVSQLSGMRGLMADPSGRIIEYPIKANF
                     REGLSVLEYFISTHGARKGLADTALRTSKSGYLTRRLVDVAQDVIVREEDCGTEEGVL
                     LFDLRDENDHLIESFEERVLGRVALKDIVDKNNGEILVHAGEEVDEKAVSLFKEREIK
                     EALVRSPLTCRTRYGVCQRCYGRNLATGRLVDIGEAVGIIAAQSIGEPGTQLTMRTFH
                     TGGIRVTGEDITQGLPRVEQLFEVRKPKKAAVLSDVYGTVEKIETVGMKKKIHVIPDD
                     EEEDQSVRTYLIPQDVKICVTEGERVQAGDRLTLGPIDPRDILRIKGIKAVQEYLLSE
                     IQSVYISQGVSINDKHIEVIIRQIANLNKVMVENAGDTTLLSGELVFLEDFEEENKKI
                     GEENKLLIEKAQDFLTDAVLVEEVVDMTGEVVLPQGKILNEDDIRLILSSDCKPFLVE
                     RKGSLLRVVRGYRNLGLELADKINVEEVKTSEGKVLLKAGESLKPEAIDRIAEMGPIK
                     LKARDKSIWEKLRGSILAEEVVDPQSGEVLLPADTRLDNETVELLDEHGVEKVVVWKD
                     VKVFPIKESLVKALREEILGKEVAEDVISPQNGEVIVQAGQAISRGIARRLVSEGVKE
                     IPLSDGRFFSIEGRLIELLEKEIVGKVIAQDIVSGSDGVVMVKAGQVIDRDDVVKIAG
                     DNIGFLLIREPDEDKEIKSLVEEILYLPGLKQVATGRPVVLGITKASLAVDSFLSAAS
                     FQQTTHVLADAAIKGKVDELIGLKENVIIGKAIPAGTGFSKYRHIRLSTEAEEKERIR
                     GEKLNEESLDVWELMGAKESFEEEEVEYNLDQGYFDDAGES"
     gene            17082..18170
                     /locus_tag="PKN31_01315"
     CDS             17082..18170
                     /locus_tag="PKN31_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOA98600.1"
                     /translation="MVLDKESLEFRKMKRLNLVARKSSNPVGENLYKQEPFYPFSEVQ
                     INGGESNRGLRRKLETPERKRKSKSFEPGKWLLERVGTPFLLGVLESLVSVLPPTLIK
                     KLAFFLGDLCYRFSGTQVAKIQWMQVMGEEDDLEVNRHIQQVFRHFALSIMEVMLLKK
                     KGSEVLDSLIEEVEGEEFLKEALSRGRGVILLTAHLGNWELLGAWLGSQGYPVTALVN
                     SPSVPALRRFAEELRLAMNIELVERTNMLPVRSILRQGKILGVLADQRASGGVVCNFL
                     GREAPSPATPAVLHLRLGSPIIPAFIVRQEEEMKHQIAFLPPLEWPSAPSHRENIVRG
                     TTLCNQVLGEFILQYPEQWNWFYHQWSD"
     gene            18709..18957
                     /locus_tag="PKN31_01320"
     CDS             18709..18957
                     /locus_tag="PKN31_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOA98601.1"
                     /translation="MSIEELRNKKIVVGKRETERAIKKNMAKKVFLARDVDEEVQKEL
                     IGILKEREIPWEYVPLAKDLGLACGIEIEASCAAIVED"
     gene            18976..19350
                     /gene="rpsL"
                     /locus_tag="PKN31_01325"
     CDS             18976..19350
                     /gene="rpsL"
                     /locus_tag="PKN31_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOA98602.1"
                     /translation="MPTVNQLVRQGRKQPKKKSSAPALKNSPQKRGVCTRVWTITPKK
                     PNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSIVLIRGGRVKDLPGVRYHVVRGTM
                     DTAGVENRKQARSKYGAKKPKS"
     gene            19364..19837
                     /gene="rpsG"
                     /locus_tag="PKN31_01330"
     CDS             19364..19837
                     /gene="rpsG"
                     /locus_tag="PKN31_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOA98603.1"
                     /translation="MPRKGPVPKRKTPPDPVYNNVDVACLINKVMRSGKKSVAEKIVY
                     GAFETIRDKTKRDPMDVLSQALNNVMPLIEVRPRRVGGANYQVPMEVRPDRSKSLGLR
                     WLVSYAQGRPGRDMVDKLAQEIMDAANGTGGAVKKREDTHRMAEANKAFAHYRWF"
     gene            19871..21961
                     /gene="fusA"
                     /locus_tag="PKN31_01335"
     CDS             19871..21961
                     /gene="fusA"
                     /locus_tag="PKN31_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOA98604.1"
                     /translation="MTNRVRSESEEHLISKIRNIGIMAHIDAGKTTITERILYYTRKI
                     HRLGEVHEGAATMDFLPQERERGITISSAVTTCFWRDFRINIIDTPGHVDFTAEVERS
                     LRVLDGAVAVFSGLEGVEPQSETVWYQADHYHVPRIAFINKLDRVGADFYAAVQSIRH
                     KLNANAHPLQVPIGKESDFRGVVDVIEMKAYLYDSEGEGTDYEITEVPSELMSEARDV
                     RHKLIEALAELDDHIMEKYIEGEDPSPLEIREAIRRVTIAGKFIPVLGGTALRNKAIH
                     PLLDAIVWYLPSPLDLPPVEGINPKTGEKEQRFASVEEPFSGLVFKIASDPHVGKISF
                     LRVYSGSLKSGSYVYNSTKGEKERVSRLLMIHASHREDVDEVRAGDLCGIIGLRKAAT
                     GDTLCDENHPIILGSLSFPEPVISVAIEPKTRADQDKLTIALSKLSEEDPTFRVRTDE
                     ETSQTIISGMGELHLEIVIDRLLREFKAEANVGQPQVAYKETIKGSAKAEGKFIRQTG
                     GRGQYGHVVLEVEPTEENFVFEDRIVGGAIPREYIPAVQAGVKEALDSGVIAGFPVVG
                     IKVKLIDGSYHPVDSSDIAFKIAGAMAVKEAIQKAEPVILEPIMKVQVIIPQEYLGEV
                     IGDLNARRGKIEGMETRGAVQVVNALVPLRELFGYATALRSLTQGRGSYTMQFSHYEE
                     VPSFVKSEIMEKSR"
BASE COUNT         6516 a         3952 c         5355 g         6193 t
ORIGIN      
        1 gatgtttttg gagtagtctg ggaccggagc atagataagg atatcggcaa tgtccagaaa
       61 ccagtgttat ctaaacccag cctacaggga tatcggtttc ccaacccttt agaccaccgt
      121 ttttttcagg atatccccga gaaaattcag cgctatcccg ataggtttcg ggtattccag
      181 atcggttttt cgctatatga gagagcttgg accttaagag gtatggagca acttatgatg
      241 gatttctatg accaccctga ttttgtgcac gagctgttac acactatagc cgactataat
      301 attgctcaag taaaagaggc tatgaagtac gatatagatg ccgtatattt tggcgatgat
      361 tggggacagc agcgaggttt gcagatgggg ccgaaaatat ggaaggagtt tatctatccg
      421 gagttacgcc gaatgtatgg aagtgcgaaa caggagggga aatatgtatt tattcattct
      481 tgcggagatg tagatgaact gttcgatgat ttgatagaaa taggggtgga ctgcttcaat
      541 ccttttcaac cggaagttat ggatattttt caattaatag aacaataccg ggatcgcttg
      601 agttttcatg gtgggctttc cactcagcga actcttccct acggcacccc tcaagatgta
      661 gtgcgggaaa caaggcgcct tatagatatg ggacgtccag gaggatatat ctttggcccc
      721 gctcacgatg tggaagggga tgttcctctt cccaacatgc tggcttttat tgaggaggct
      781 caaaaacagg tatagtctcg tcaattttct tcaggtaaga aaagatttca tctttttcct
      841 aattttatag tatctcgtga aattcctggc atgttcttct ttttaatact ccacctcttc
      901 actgtgtcct tggggtatta atcgcacgct gagggagatg cttttactcc ttcagcagtt
      961 ttgtttttct atgttttcag atttttctct cccggaagtc ataggaggat ttgtgtccta
     1021 tagttcgaga gatgtttctt ctctctaagt gctggaatgt tattacttca gcgttgttat
     1081 tccaaatttt ttcatctttt gtgctctctt agtcttatac cacaagctat ctgggcaatc
     1141 tctcagaggc aaagcaagct cataacatca attgtgtggt aatatcggga tgacagagta
     1201 tcccacttat ttgtagttgt gctttttgaa tctctcttgt agaaggatgg attttgtgct
     1261 aaattccttc cagaaataat ttatcggggt tggtgctatg aaaagaaaat taagagtggc
     1321 tgtcatctgt ggtggaaaat cggtagagca cgagatttct ctccaatcag caaaaaacgt
     1381 tatagaagct atggatagag agaaatatga tattgtcctt ataggtattg ataaagacgg
     1441 tcgctggcac cttaaagctt tagctgattt tgaagaagaa atggaaggct ctcttctgcc
     1501 tcatcttacc tctcgagggg atgaggtgat ttttattccc ggaggcgcag gacaactggt
     1561 taatctctct actggagaaa ggttgcagcc ggtagatgtt gttttccctg ttcttcatgg
     1621 accgttcggt gaagatggtg ctatccaggg attgctgaaa atagcagaag ttcctttcgt
     1681 gggggcggga gtgctaggtt cagctattgc tatggataaa gaggtaagca aaagattgct
     1741 gaaagaagcc ggaattcctg ccgctaaatt tttatctttt gctcgtgagg aaaaagagga
     1801 aatcaaattc cccgaaatgg ttagccaatt aggattgcct ttttttgtaa aaccggccac
     1861 tcttggctca tctgtgggta taagcaaagt taaaaaagag gaggagtttt tctctgcttt
     1921 agaggaagct tttcgctacg atggtaaaat cttaattgag gaatatgtcg aggggcgaga
     1981 aattgaatgt gccgttctgg gaaatgaaaa acctcgtgcc tcttgccctg gagaaatcat
     2041 ccccggtcat gaattttact cttacgaggc aaaatacatt gacccggaag gagctcttct
     2101 tcagattcct gctcctcttc cagaaaatat agcagagaaa atacaatgcc tggccattca
     2161 ggcttttcgg gctctctctt gtgaggggat ggccagggtg gacttttttc tccggggaga
     2221 agaaattatt gtaaacgaac tcaacaccat tcccgggttt acttccatca gtatgtattc
     2281 caagctatgg gaagcaagtg gcatccctta ccctcagctc atagatgagc taattcaatt
     2341 agcgttagaa agaagtgaaa aagagaagtc gttaaaaact ttcataaatt ggtaacaaat
     2401 cgctcactga aaagccaaaa agacccctta aaatgttctc aaaagctaaa aaggaggtta
     2461 aacacatgag aaaaacctgg ttatttgtag ctttggtgag cgtgcttgtt ttaagtatag
     2521 ctttccccat ttttgctcag gaaagaagct cccctccccg tgaggaaaga gatagggaaa
     2581 tgagacagga taggctgcac cagaatatta actattctct taacctgagt caggaacaga
     2641 aaatcaaaat cctggaggtc tgggaaaaat atcgtccgaa catccgggat ttgcaagacc
     2701 agatggagga agcgcgaatt agtctctgga aattgagttt tgaaactcct tctgaagaag
     2761 tagccagtga gatacggact aagatagaag aaattaactc cttggaacaa aagatacagg
     2821 aaagcagaaa tgctatgttt caggaagtgc tttctattct gactcccgaa cagacttctc
     2881 aattggctaa gcaatatccc caaagaagag gaaaaggctt tacctttcag agagacacgg
     2941 gctttccagg tatgggcttt ccggcaaaca tcaggtagga taattatagt agccccgaag
     3001 tagcttcggg gctactataa cgcacaacac ggttcctggc cataagaata ccaggaggag
     3061 agaacctggg aaaatcagtt aaaggagttt tgagagaaaa tagtcggcga gcattattaa
     3121 atttcttata tttaaaggtg gatagagtat ggctattatc ctagtagtgg aagatgaaga
     3181 aagtgtgaga gagctcctcc gcttgtatct tgagaaggag ggatttcagg tagaattggc
     3241 ttccgatgga gaggaagcac tgaaaaagat gtccaggatt tctcctgatc tcgttctttt
     3301 agacctcatg cttcccaaaa aagatggatg ggaagtgtgc cgggaaataa ggaaaacttc
     3361 ttatgttcct ataattatgc tcaccgcccg aggggaagaa tttgataagg tgctggggtt
     3421 ggaactggga gctgatgact acgtggctaa acctttcagc cctcgggagt tggtagcgag
     3481 gattaaggct gtccttcgtc gctttaatgc ctcttcgggg ggaggaaaaa cgtgggaagc
     3541 tcttggtctc cggattgatt atgaagccaa aaaagtggaa ataaaaggga aggaaatcaa
     3601 cctcactccc aaagaattcg aactactctg gtttctggta aaccaccccc agagggttta
     3661 cacccgggaa cagcttttag aatatgtgtg ggggtatgat tttgcaggag atgctcgaac
     3721 tgtagatacc cacataaaac gtcttcgaga aaaattaagc gaaggaggaa tttcttcttc
     3781 aataaaaacg gtatggggat acggttataa atttgagccc gaacgatgaa aaaaacccta
     3841 ttttctaaac ttctcctttc ttatcttgca gtggtggccc tttccttttt ggtctccgga
     3901 atcttaagtt cccaattttt ttctcgccac taccttgctt cccaagaaaa agaaatagcg
     3961 gaacaggcta aaaccatcag cgaattcatt tcccagtctc ctcgggaaga aataccggag
     4021 attttacccc gcctgagaaa aggaactcag attttgcttc tcagccgaca ggaactatcc
     4081 tctcctaatt ttcctctatc cgagccacct cgccccccct ggggtagaat gggtagagag
     4141 ccgcttcccc gagaaatgtt agaaaaagtt tttcaaggag aaaccgtagt ttttagaggg
     4201 ttcagccccc gttttgacca ggcaatgttg atagccgctt ccccggtctt gcaaaatgga
     4261 gaagtttctt ctgttctttt acttttatct cctatagtta atattcaggc tactgtgtct
     4321 gcggtaaggc gtatcatcct tatagccagt gccatctctt tagttttttc tctcattctc
     4381 tcctattttt tctctcgctc tctcactcgt cctcttcaga aaataagcga agcagctcga
     4441 gatttagctt ccggcaattt acagcggaga atagaagtgc acacggaaga tgaattggga
     4501 caattagcgc aagattttaa ttacctttcc tccgctttag aagaaaccat taatcacctt
     4561 catcaggaaa aacgggaaac cgaaaacata ctccttcata tggcagaagg tgtagtagct
     4621 atagatgaag aagagaaaat cacttctatt aatccagcct tacttaaaaa attgaagtgg
     4681 gagggagaag ctcctttggg acttcatgtt gcccaattat ttactcaagt ccagatacag
     4741 gaaattttct cccgagcgct aaaagaggga aaagaagaag ctcaagaatt tcaagtagaa
     4801 aatggagacc atttcgttgt ccacataaca cccttaaaag agggggagaa agtatatgga
     4861 gcggtagggg tttttcaaga tatcaccgaa cttcgccaac tggaagaact acgtcgtgat
     4921 tttttagcta acgtttctca cgagttgcgc acccctctta cctccatcca gggatttgta
     4981 gaagcaatga tggatggggt aatcggtgat gaggagctaa aaaagaaata cttagaagtt
     5041 attcaccgag agacgctgcg cctttctcgg cttattcatg accttttaga cctttctctc
     5101 atggaatcaa aaaaaataga atgggaagta aatcctttat cccttcccca gatgatagac
     5161 caggtattgc ttaagttaat gccccagata gaggataaaa aaatccagat aaagaaagat
     5221 tttccttctc agctcccttt agttctggga aatatggatc ggattgagca ggtgttaata
     5281 aatttactga gtaatgctct tcttttttct cctccggaga gcaccgtaga aataaaggtc
     5341 tgggaagagg agaaagaagt agctgtttct ataaaagacc agggggaagg aatccccgaa
     5401 aaagatatcc cccatctctt tgagcgattt taccgggtag aaaaatctcg ttctcgagaa
     5461 aaagggggga caggcctcgg gctggccatc gccaaacaaa ttatagaaaa ccacggtggt
     5521 acaataaagg tggaaagcca gctccaggag gggactgttt ttacctttac tctaccgata
     5581 gcttctgaat ccggcggaga tgacacgata gactcctaac tggtcgcaag ttttttcttt
     5641 gaaaaccttg ttttttagaa gtaagacttt tccttggcta ttccttgcag acaagattgg
     5701 agagacacaa gcattccctg agatagggag aatttgaggg tgaaaaaaat taaagtttta
     5761 acctatttga taactgcagt aaactcccgg gagagagcag gctattttcc accctctccc
     5821 ggtaaaattg acgtctcggt ttattcgata aattgttctc ctttttcagt aagtttatac
     5881 tttccttctt cttctttcac taaatcggct tgaaccatgt ctcgaagtcc acttcttact
     5941 cggaatagag gtaaaccact ggttttagct atttcttcag gagacgctgc tccagatttt
     6001 agagcgttga gaattttctt tcccgattca gtaggttttc cttctggcgt tacgcaggcc
     6061 ataaaaaccc tcctttctta gacgcttttt ttacatagat accaatctac acattcatat
     6121 ccctctttta accgctcttc tgctccttct acggtggtgg gggcgggaac tattactttt
     6181 tctcccggtt gccagttagc aggagtagcc acaccgtatt tatcggtggt ttgtaaagca
     6241 tcgattattc tcaatatttc actgatattt ctaccagtag ttagagggta atagaggatg
     6301 gcgcgaataa cctggtcggg gtcgatgata aacacacacc gaatagtttc agttttgctt
     6361 tctccggggt gaatcatgcc gtatttgata gatacttctt tactaaggtc ggcaataatg
     6421 gggaaaggaa ctttgactcc ggttttctcc tgaatgttgc gagcccaggc aatgtgagag
     6481 gacacgctat ccacgctgag tcccaggagc tctgctcctc ttttttgaaa ttcaggatag
     6541 gcctggctaa aagcaataaa ctcggtagtg cacaccggag tgaagtcggc aggatgagaa
     6601 aataacacca gccagctccc tttgaaatct tccagcctta aagttccatg agtagtaaca
     6661 gcttcaaaag agggtgccat ctctcccact cgaggaaaac tgataatttc ttctcttaat
     6721 tcttccatcg tttatacctc ctctttattt ttatctccgc taggggagat aattgctttt
     6781 ttctgacaat cagagcaaaa gccccgaaac tctaaaacat ggtgagtaat gttaaacccg
     6841 gttttttgag ataccttttt atccagttcc gtatctactg gcacatctac atcgaacacc
     6901 tttccacaac tctcacagcg aaaatggtaa tgattttcca ctcgggcatc atatctacct
     6961 tctgttcctc ctgtatcaat tttcataact tctttgtttt cggtgaggag tttcaaatta
     7021 cgatatactg tgcccttact aatgcgagga attttctgcc gcactttttg gtaaacttca
     7081 tcagcagtgg gatgagattt agtatttttt agaatttcta aaatagcttc tctttgttta
     7141 gttttcttaa ttttattcat ttcttccatt ataataatag ttctcattac tatttactta
     7201 gattatacat aatgtttaat tttttgtcaa atttttatga gaccgtttgt agttattttg
     7261 aggctgtttg aaaaatccct ttctatagtt aaaaagtaat aaagtcattt catgcatacg
     7321 atgttttttt ctcctgcaaa aggaatttta taaaccttca tagtgataat tattaaatga
     7381 aggaggcaat atatatctct aagttttttt aatcaggttt atttcaaata agtttttact
     7441 tcccttttct attatccggg ggaaagggaa ttcaaagcaa atgggaatgg ctctttcttt
     7501 ctaaaggtac agaggcagat aaaacgtctg gtattctgta taattcaaga ccatagcttg
     7561 aagcttttcc accacttttt ggacaaaaac ttccagtatt tttctatagg tctctaaggg
     7621 ttgggcaaat aatctgctta acatcttctt ttaagagaga agctttttga agatagaagc
     7681 tgtctggcta tctctgaaat aataaagctt tttgcctcaa tagccctgag attaaaagaa
     7741 ccagtcttct ttaagctggt ttctttaaat tcatctgcaa ggttacttga aatatttcac
     7801 aaaattttgc ataatatata ggaatgttaa ttcgtaaatt ccctttgggg ttatttatat
     7861 tcgttcgtga gttgcattta tatcccgcga gagggttgtt ttttagagac cttccaaaag
     7921 tgcctcaagt caggcctttg tgctattgac atttgattaa aaagcttata taatagctat
     7981 ttgtcttttc aggtgtattt aagacgggag gacgaaaagt tattattctt tcttcaaaca
     8041 aagtgttatc taacaaaaat tgctactcga ttttccctca aaaggtggtg tgattagtga
     8101 gtgtaagtta tattgagagg caaagttttg ccaaaatccc ttcttccttg gaacttcctg
     8161 atttcataga gattcaaaaa aagtcttttg attggttttt gcagggagat gtcccacctg
     8221 aaaaccgtaa aaagcaagga ttgcaggaag ttttcctgga agtgtttccg gttcaagatt
     8281 ttactggaag aatggttctc gagtttgtca attatcgatt ggaagagcca gtttttactg
     8341 ttaaagaggc taaggaaaaa gggagaactt attctgttcc tcttcatgcg cggataagat
     8401 taattgaccg gcaaaccaat gaaataaagg aacaggatgt gtatatgggt gatatacctc
     8461 tcatgaccga atggggtact tttgtagtta atggagccga acgagtggta gttactcagt
     8521 tagttcgctc tcccggggta tttttctctc gagaagttac taccaccggg aaagtggatt
     8581 acggctttaa gattattccc aatcgggggg cgtggttaga atttggaatt gatacaggag
     8641 atatcattta cgtacgagta gataaaagga gaaaaattaa cgctgtcact ctggttagag
     8701 ctctgggata tgaagataac gaggaaattt tgtctatgtt tgattatcat ccgattatcc
     8761 ggaagactct ggagaaagat ccttctacta attacaaaga agcagtggta gagatctttc
     8821 gtagacttcg tcccagcgag cctcctacgg aggagaacac cagagatttc atgagatacc
     8881 tcttttttga ccctcgtcgc tatgatctgg gtgaagttgg tcgctataaa ataaataaca
     8941 agctaaagtt ggaagaaaga gttattcgga tagaagaacc agtgttggct gaggatattg
     9001 tgattgactt gcaggataaa aaatgcctgc gagatatcag cttactgggt gaagatattg
     9061 atacttctcg ttatcgaata gcaggaaaaa agggagaaag aattaaccgt cgcctggccc
     9121 aggaattgga aaaacttaac gaagaatacc ccatagaata cataaaagtg tttagcgaag
     9181 aagaattacc ggtgaaaatc tatgtggaaa gagaggacac ctgggaggtt ctttctgagg
     9241 atctggaagg aagaattttg agtgaagcgg tagtggatga gataactgga gaagttctgg
     9301 tagaaaaagg aaaaccttta actctgggtc ttatccggga tctgcgagga ttaggaatag
     9361 aaagagtgag ggttaaaaaa taccgaactc tcactcccga ggatgtagtg ggcagtattc
     9421 gctatttcct taatcttatt gatggtattg gttatgaaga tgatattgac caccttggca
     9481 accgacgagc tcgtatggtg ggagaacttt tacaaaacca gttccgcatc ggtcttttgc
     9541 gagtagagaa agtgatcagg gaacggatga ctattcaacc tgatgtggaa tcagccactc
     9601 ctcaaagtct cattaatgtg aggcctattg ttgctgctat gcgggagttc ttaggcagca
     9661 gtccactatc tcaattcatg gatcaggtta atcctctttc tgagattact cataagcgaa
     9721 gattgagtgc cctagggccg gggggcttga gtcgagagcg ggcaggtttt gaagtcaggg
     9781 acgtgcatta tacccattac ggaagaatat gtcctattga aactccagaa ggccctaata
     9841 ttggtcttat tacttctctg tgcatgtttg ctaaggttaa tgattatggg tttatagaaa
     9901 ctccttatcg aaaagtggta gatggaagag ttactgatga aatagtttac ctttctgccg
     9961 atgaagaaga cctttatcac actgcccctc ccgatgttcc cctggatgag gagggctata
    10021 ttcagacgga gaatacaata gcccggttca gggaaaaaat agtggaagtg cctcgtgata
    10081 aggttgattt tatggatgtt tctcctcagc agattattag cgcttccgct gctttgattc
    10141 cttttttaga gcataacgat gctaaccggg cgttgatggg tactaacatg cagcggcagg
    10201 ctgttcctct tttagtttct catcctccct acattgggac agggatagaa tacaaggtgg
    10261 cgcaagattc aggagccacg gttatagcta aaagaggcgg agtggtgaag aaagctgatg
    10321 ctttgcaaat tgaaatagaa gcaaatgatg gcggaataga tgtgtatgaa ttaaataaat
    10381 ttcagagatc caaccagagt acttgcatta atcataaacc aatagttaga aaaggagata
    10441 aggtggaaaa ggggcaaatc attgctgatg gcccttgtac ttccatggga gagctatccc
    10501 tggggaagaa cgttcttgtg gctttcatgc cctgggaggg agaaaacttc gaagacgcca
    10561 tcatgattag tgaaagattg gttaaagaag atgtttttac ttctatccat atcgatgaat
    10621 acgaagtgga agcccgggat acaaaattgg gtccggaaga aattacccgg gacattccca
    10681 acttaggaga ggatatgcta cgcaacttag acgaagaagg aatagcgata ataggctcgg
    10741 atgtgaagcc gggagacatt ttggtgggta aagtgactcc caaaggagaa accgagctta
    10801 ctcctgagga aaagctcttg cgagctatat ttggagaaaa agctcgagag gtcagagata
    10861 cctcacttcg tattcctcat ggagagtatg gcaaagtcat agatattaaa gttttctccc
    10921 gggaaaatgg tgatgaatta gctccgggag tgaacaagct ggtaaaagtt tttgtggctc
    10981 aaaagagaaa aatctcggtg ggagataaaa tggctggcag gcatggaaac aagggagtag
    11041 tagcccgaat agtccgagag gaagacatgc cttaccttcc cgatggaaca ccggtggata
    11101 ttgtgctcaa tcctctgggg gttccttccc ggatgaatgt gggacaggtt ttggaaactc
    11161 acttagggtg gatagctaac aaggaaagac agtttttgtc cagccctccc tttgatggag
    11221 cgtgggaaga agaaattatg gagcttatga ggaaggttga agctccggaa gaacaaggtc
    11281 ttttcgatca cttttttgat tcacggattt cttccgattt taaacttctt cctcagggca
    11341 aaactattct ctatgatgga agaacaggag agccttttga taacgaaatt actgtaggtt
    11401 atatatatat gctcaaattg gctcatcttg tagaaaccaa aattcatgcc cgctctaccg
    11461 gtccttactc tttggtaact caacaacctc tgggaggaaa agcccagttt ggtggccaaa
    11521 gatttggcga aatggaagta tgggctctgg aagggtatgg agcagcctat actttgcaag
    11581 agatgctaac tgtaaaatct gacgatattg cagggagagt acaggcttat gaagctattg
    11641 ttaaaggtaa gaatattctt aaaccttctc ttcctgagtc tttcaaagtg ctggtgaaag
    11701 agttacagag cttgtgtctt gacgtgagag tatttgattc taaaaagaga gaaatttctc
    11761 tggaggaagt agaagaggaa gaagatactc ctattctgga tattaacctc cagagaagag
    11821 aaaaataaaa tagaggagga gaatccttga tagacgttaa tgatttaggt gctatccaaa
    11881 ttaaattggc ttctccagaa gaaattcgta aatggtctca cggagaagta aaaaaacctg
    11941 aaactataaa ctatcgaact ctgaaaccag aaagagatgg acttttctgt gagaaaatat
    12001 ttggacctac tcgagattgg gaatgctatt gtgggaagta taaaagagta agagatagag
    12061 gagtagtatg tgaccgctgt ggagtggaaa taactcgttc ttcagtaaga agagagagat
    12121 tgggacacat cgaattagct gctccggttt cccatatctg gtattttaaa agtattccca
    12181 gtaaaatggc tcttatttta gggattttac ctaaaaatct ggaaaaagta ctgtactttg
    12241 cctcgggccg gaaaagagaa gattgttata aagtagtgga gccgggctct actgatttgg
    12301 aagcagggtc taccatccgg gatatagagt ataaaatcta ttctaactat gatcccaatt
    12361 ttaaagctga acctgcttac cggattgcag aagtttacag ccttcctttt accattggag
    12421 atcggctgag tgttaaagaa tatactcgtt atcgctccaa atataaggaa agtttcaccg
    12481 tggaagaagt agaagaagac cagtttgagg tagtagacat tcgagtattt ccttatcagc
    12541 gagaagatga gctttctgag tcggaagtta aagagttaca agaaattttt ggtggactct
    12601 ttgaggagac tcctttatct gaaacagtgg aagaaccttg ttatatagta attgatcccg
    12661 ggcaaacggg tttgaaggta gcgcagataa tactggaaac ggaggcccgg ttgttcaccc
    12721 actacgattc cacatttaaa gcaggggtag gagcagaagc ggtaagagaa attttaagca
    12781 atattgatgt aaaccagtta gctcgagaat tacgcaaaga acttaaggag gcttctggcc
    12841 agaaggctaa aaaactcatc aagagactgc aggtagtgga gtcttttgct aaatcgggta
    12901 atcgtccgga atggatgatt ctcgagacta tacccgtttt gcctcctgat ttgcgtccta
    12961 tggtgcagct ggacggagga aggttcgcta cctcggactt gaatgacctt tatagacgag
    13021 taattaatcg aaataaccgc ctgaagagac ttctccagtt gggtgctcca gaaatcattg
    13081 tgaagaacga aaaaaggatg ttgcaagaag cggttgatgc cctttttgat aatggtagga
    13141 gaggtagacc ggttctaggg ccgggcaatc gccctttgaa gtcacttagt gatatgttga
    13201 aaggaaaaaa aggaagattc agacaaaacc tcctgggcaa aagagtagac tattcgggaa
    13261 gatcggttat tgtttccggg cctcgcctgg cttttgacca gtgtggtatt cccaagaaaa
    13321 tggctctgga gctttttaag ccttttgtta tgaagaagtt agtggatagg ggctttgccc
    13381 aaaatattaa aagcgccaag aggatggtag aaaaagcaag ggatgaagaa gtatgggcag
    13441 ctttagaaga ggtgatagct gaacatccgg ttctactcaa tagagctcct actcttcacc
    13501 gattgagcat tcaggctttt caacctgttc tcgtggaagg taatgcaatt caattgcatc
    13561 ccttagcctg ttcggctttt aatgctgact ttgatggaga ccagatggcg gtacacgttc
    13621 ctctttctgc tgaagcacag gctgaatcgc aaattatcat gctttcttct cataatgttc
    13681 tttctccagc gagcggtaag cccgttgctg ttcctaccca ggatatggtg ttgggtagct
    13741 actatttaac tttatgggga ggcggagaaa gtagaagata ttttgtagat gtagatgaag
    13801 ctcttattac ttacaatctt ggcaacctgg atttacacga accaattaga attagagaaa
    13861 ttagtgaaaa atacccggaa atagaagaaa ccactctggg tagagctata tttaatgagc
    13921 tattacccga tgctatgcct tattacaatg aaccagtaac taaaaagaaa ttgtctgaaa
    13981 ttgccgatct ctgttatcga aagctgggta acgaggctac ggtaaccgtg ttagacaaga
    14041 ttaaaaaggc tggttttcat tttgctaccc gttcgggaac aaccatcagc gtgcaagatc
    14101 tggaaattcc tcctgaaaag tggagtatca ttgaagaagc taaccggcgg gtggaggaaa
    14161 tcaatcaaca ttatgctgag gggctgatta ccgaagaaga aagagaacag aaggttatag
    14221 acgtctggac taatgcttct aataaagtgg ctgatgctat ccttgaagag ctggatgaat
    14281 tcaacccggt taacatgatg gctaagtcgg gagctcgagg aagcattcgc caggttagcc
    14341 agctatctgg tatgcgagga ttgatggctg atccctcagg aaggattata gaatatccca
    14401 taaaggccaa tttccgagaa gggttgagcg ttctggagta tttcatttct actcacggag
    14461 caaggaaagg tctggctgat actgctttac ggacttctaa atccggttat cttactcgcc
    14521 gtttagtgga tgtggctcag gatgttattg ttagggaaga ggattgcggg acggaagaag
    14581 gagttctgct ttttgatttg cgagacgaaa atgaccacct tatagaatct tttgaagaaa
    14641 gagttttagg aagagtagcg ttaaaagata tagtagataa aaataatgga gaaatattgg
    14701 ttcacgcggg agaagaagtt gacgaaaaag cagtctcctt gtttaaagaa agggagataa
    14761 aggaagccct ggtgagatca cctcttacct gccggactcg ctacggggta tgccagaggt
    14821 gttatggtag aaatttagct accgggcgat tggtggatat aggagaagcg gtagggataa
    14881 tagctgctca atcaattgga gagccgggga ctcagctcac catgcgtact ttccatactg
    14941 gtggtattcg agttaccggt gaagatatca ctcagggttt gcctcgggtg gagcagcttt
    15001 ttgaggtgag aaagcccaag aaagccgccg tcctttccga tgtatatgga acagtggaaa
    15061 aaatagaaac ggtaggaatg aagaaaaaga tccacgttat tcctgatgat gaagaagaag
    15121 accaatcggt gcgtacttat cttatccctc aggacgtaaa gatttgtgta actgagggcg
    15181 aaagagtgca ggcgggggat cggcttacct tagggcctat tgaccctcga gacatattga
    15241 gaatcaaggg tattaaagcg gtacaggaat acctcctcag cgagattcaa tcagtttata
    15301 tctcgcaagg agtgagtatt aatgataaac atatcgaagt tattattcgc cagattgcta
    15361 acctcaataa agttatggtg gagaatgctg gtgatactac tcttctgtcc ggggagctgg
    15421 tatttctgga agattttgaa gaggaaaaca agaaaatcgg agaggaaaat aagcttttaa
    15481 tagaaaaagc acaagatttc ttaactgatg ctgttttagt ggaagaagta gtggatatga
    15541 ccggtgaggt tgtactgccc cagggcaaaa ttctcaatga agatgatatc cggctaattc
    15601 tctcttctga ttgcaagcct ttcttggtgg agagaaaagg tagtttgctt cgagtggtcc
    15661 ggggttatcg taatctgggg ttggagctag cagataagat taacgtagaa gaagtcaaaa
    15721 catcagaagg taaagtttta ctaaaagctg gtgaatcgtt aaagccagaa gctatagacc
    15781 ggatagcgga gatggggcca attaaattaa aagcaaggga taaatctatc tgggagaaac
    15841 ttcgcggatc aatactggct gaagaagttg tcgaccccca gagcggggaa gtacttcttc
    15901 cagctgatac ccgattagat aatgaaactg ttgaactact tgatgaacat ggagttgaga
    15961 aggtagtagt gtggaaggat gttaaagttt ttcctatcaa agaaagttta gttaaggctt
    16021 taagagaaga aattctggga aaggaagtag ctgaggatgt aatcagtcct caaaatggtg
    16081 aagttattgt tcaggctgga caggcgataa gcagaggcat agctcgtcga ttagttagtg
    16141 agggggtaaa agaaatccct ctctctgatg gtcgattttt ctccatagaa ggtagattga
    16201 tagagctttt ggaaaaggaa atagtgggta aagtgatagc tcaagatatt gtatccggta
    16261 gtgatggagt ggtaatggtg aaagccggac aggtaatcga ccgagatgac gtggttaaaa
    16321 ttgctggaga taatatagga tttcttctca tccgagagcc ggatgaagat aaagaaatca
    16381 aaagcctggt agaagaaata ttgtatttac cagggttgaa gcaggtagcc actggaaggc
    16441 cggtggtttt gggaatcacc aaagcttctt tagcagtaga tagttttctt tccgccgctt
    16501 cttttcagca gactacccac gttttagcgg atgcagccat caagggcaaa gttgatgaac
    16561 tgattggtct taaagaaaat gtaatcattg gaaaagctat ccctgctggc actggcttta
    16621 gcaaatacag acacatccgt ctttctactg aggcagaaga gaaagaaaga ataagaggag
    16681 aaaaattgaa tgaggaaagc ctggatgtgt gggaattgat gggagcaaaa gaatcatttg
    16741 aagaagaaga agtggaatac aatttggatc aaggctactt tgacgatgcc ggggaaagtt
    16801 agggtttccc cggcaaatca cgttttcagg ggtttgcaaa ggtctgtggc tctcatataa
    16861 taatttatat ataactgtgg gattaactct gttggagcct actgagaaag aacccggata
    16921 caaaaggtaa gaaaacggaa acaaatcaaa aatgtaaaat acagaaagag aattgaaagg
    16981 atagcaccgg ggaaaatctg cggtaatcct gcgtttttaa ctctctttaa gaggcaatag
    17041 tactttgtaa agcaagaata tccaggaggt agcggtttat tatggttctg gataaagaat
    17101 ctttagaatt tagaaaaatg aaacggctca atcttgttgc caggaaatca tcgaatccag
    17161 tgggggagaa tttatataaa caagagcctt tttatccttt cagcgaggtg caaattaatg
    17221 gtggggaaag taaccgggga ttaagaagaa aattagaaac acctgagcgg aaaagaaaat
    17281 ctaaatcatt tgaaccggga aagtggttac tggagcgagt agggactccc tttctccttg
    17341 gggtgctgga gagtctggtt tcagttcttc ccccgacgct tatcaaaaag cttgcttttt
    17401 ttctggggga tctatgctat cgcttctccg gcacgcaggt ggctaaaatc cagtggatgc
    17461 aggttatggg ggaggaagat gatttagagg ttaatcgcca tattcagcag gtatttcgcc
    17521 actttgctct atctattatg gaggtgatgc tcctgaaaaa gaaagggagt gaggtgctgg
    17581 attctttaat agaagaggta gagggagaag aatttttaaa agaagctcta tcccgggggc
    17641 gaggagtaat tttactaact gctcatttag gaaactggga acttttagga gcctggttgg
    17701 gaagtcaggg ttaccctgtt actgctctgg ttaattctcc gtcggttcca gctcttcgga
    17761 gatttgcaga agagctgaga ctggccatga atatcgagtt agtagagcgg actaatatgc
    17821 ttccagttcg ctcaatcttg cggcagggga aaatattggg ggtactggct gatcagcgtg
    17881 cttcaggagg agtggtttgc aactttttag gtcgggaagc accttctcct gctaccccgg
    17941 ctgttttgca tcttcgatta gggagtccga ttatcccggc ttttattgta cgccaggaag
    18001 aagagatgaa acatcaaatt gcttttcttc ctcccctgga gtggccttct gctccttctc
    18061 atcgggaaaa tattgttcgg ggaacgactc tctgcaatca ggttttagga gagttcattc
    18121 tccaatatcc cgagcagtgg aactggtttt atcaccaatg gagtgattaa cttagggttt
    18181 tacgttttta ctaagtttat cgaatccgat atttgattaa attactcttt ctattttttg
    18241 ctttagaggg cttctagttt aatagaagcg cctttgaata atagccaagc attattatgt
    18301 ttttagcaag ctggcctcgg gcgaaaatcc tcctcaaaaa caccggaggt gctggcaagg
    18361 gcgagggaaa gaagaggagt ttcgcatctt ctttttggaa aagaattacc ttctgtaaaa
    18421 aggattttcc tcaaagcggt caaagttttt tcttttgctc tgggacaata aaaagccagc
    18481 tatcgatttc cccggagagt ttaactaaaa gacttttcct gggcgagctg gaatttttca
    18541 tttagagatg atatactacc tgaggccata tggagggggt ttaagctcta catagaagag
    18601 aaaaataaaa atactcctgc gatagtttgt aatcatagcc tggaaacagt tgacaggttc
    18661 tacaaatgtt gctaaaattg ctccgttgct tttgggggtg tgtttttttt gagtatagag
    18721 gagttgagaa ataaaaaaat agtggtggga aagcgagaaa ccgaaagagc aataaagaaa
    18781 aatatggcga agaaagtttt tttggctcgt gatgtggatg aagaagtgca aaaagaatta
    18841 atcggtattt tgaaagaaag agaaataccc tgggaatatg tgcctctggc taaagattta
    18901 ggattagcct gtggtatcga aatagaggct tcttgtgcag ctattgttga agattaagaa
    18961 taagggagga ataaaatgcc aactgttaat caactggtaa ggcagggtag aaaacaaccc
    19021 aaaaagaaaa gcagtgctcc agctttgaaa aactcccctc aaaaaagagg ggtttgcacc
    19081 agagtgtgga ctatcactcc taaaaagcct aattcggctt tacgtaaagt ggcccgggtg
    19141 aggcttacca atggaataga agttaccgct tatattcctg gtatcgggca taatctgcag
    19201 gagcattcca ttgtcctcat tagaggaggg agagtaaagg atttgcccgg ggttcggtat
    19261 cacgttgtcc gggggactat ggatactgcg ggagtggaaa atagaaaaca agctcgctcc
    19321 aagtatggag ctaaaaagcc taaatcttga ggaggtgaag aagttgccca ggaaaggtcc
    19381 agttcctaaa agaaagacac ctcctgatcc agtctacaat aatgtagacg tagcttgttt
    19441 gattaataaa gttatgcggt caggaaagaa aagtgtggcg gaaaaaattg tttacggggc
    19501 ttttgaaaca ataagagaca aaaccaaaag ggaccctatg gatgttttgt cccaggcttt
    19561 aaacaatgta atgcccctta tagaagtaag accccgtcga gtgggagggg cgaactacca
    19621 ggttcctatg gaagtcaggc cagataggag caaaagtctg gggttgcgct ggttggttag
    19681 ctatgctcag gggcgaccgg gaagagatat ggtagataaa ttagctcaag agataatgga
    19741 tgcagctaat ggaacaggtg gagcagttaa aaagagagaa gacacgcacc gaatggctga
    19801 agctaacaaa gcttttgctc attatcgttg gttttaatag gaggtcagga attttaaggt
    19861 gagtaaaact atgacaaata gagttcgatc agaatctgaa gaacatctta tttctaaaat
    19921 tcgtaatatt ggaataatgg ctcatattga cgcaggtaaa actacaatta ccgagcgtat
    19981 cctttactat accaggaaaa tccaccgtct gggggaagtt catgaagggg cagccactat
    20041 ggactttcta cctcaggagc gggagcgggg tataactata agttcggcag ttactacctg
    20101 tttctggaga gattttcgta ttaatatcat tgatacgcca ggccacgtgg actttactgc
    20161 agaggtagag agaagcttgc gagtgttgga tggagcagtg gcagttttct ctggattgga
    20221 aggggtagag cctcaatcgg aaacagtctg gtatcaggct gaccactatc acgtgcctcg
    20281 tattgctttt attaataagc tggatagagt aggagcggat ttttatgctg cagtccagtc
    20341 tattcgtcac aagctgaacg ctaacgctca ccctcttcag gttcctatag gtaaggaatc
    20401 tgattttcgg ggcgtggttg acgtaataga aatgaaggcc tatctttatg attcggaggg
    20461 agagggcaca gattatgaaa taaccgaagt tccttctgag ctcatgagtg aagcccggga
    20521 tgtcagacat aaattgatag aggctttagc agagttagat gatcatataa tggagaaata
    20581 catagaagga gaagatcctt ctcctcttga gataagagaa gctattcgac gagtaaccat
    20641 agcgggtaaa ttcattcctg ttttaggggg gactgctctc cggaataagg ctatccatcc
    20701 cctattggat gctatcgtct ggtatcttcc ctctcctctg gatttgcctc cggtagaagg
    20761 gattaatcct aaaaccgggg agaaagaaca gagatttgct tcagttgagg agcctttttc
    20821 tggcctggtt tttaagattg ccagtgatcc tcatgtgggg aaaatatctt ttttacgagt
    20881 ttactctggt tctttgaaaa gcggtagtta tgtttacaat tctactaagg gagagaaaga
    20941 aagagtaagt aggttgttaa tgattcatgc cagtcataga gaagatgtag atgaagtaag
    21001 agcaggagat ttgtgtggta tcataggttt gagaaaagct gccactggtg acactctctg
    21061 tgacgaaaat caccctatta ttttaggatc tctttctttc cccgaaccgg ttatctcagt
    21121 agctatcgag cctaagaccc gggcagatca agataaacta accattgctc tttccaagct
    21181 atctgaggaa gatcctactt ttcgagtgcg taccgatgaa gaaacttccc agacgataat
    21241 ttcagggatg ggagagttac atcttgagat tgtaattgat cgcctgttac gggagttcaa
    21301 agcggaagcc aacgttggtc agcctcaggt ggcttataaa gaaactatca aagggtctgc
    21361 taaagcggag ggtaaattca tccgtcagac tggtggaaga gggcaatacg gtcacgtggt
    21421 tttagaagta gagcccacag aagaaaattt tgttttcgaa gaccgtatag tggggggagc
    21481 tatcccgcga gagtatattc ccgctgttca agccggagtg aaagaagctc tggacagtgg
    21541 tgtaatagct gggtttccag tggtggggat taaagtaaaa ttaatagacg gttcttacca
    21601 cccggtggat tcttcggata ttgcttttaa aattgccgga gctatggcgg taaaggaagc
    21661 tatccagaaa gctgaaccgg ttattttaga acccattatg aaagttcagg ttatcattcc
    21721 tcaggagtac ttaggagagg taataggaga cttgaatgct cggcgaggaa aaatcgaggg
    21781 aatggaaaca cgaggagcag tacaggttgt caacgcttta gttcctctca gggaactgtt
    21841 tggctatgct actgctttgc gttctcttac tcagggaaga ggtagttaca ccatgcagtt
    21901 ttcccattat gaagaagttc cctcttttgt taaaagtgaa attatggaaa aaagtagata
    21961 gaaggaggat agatggatta tggcaaagca aaagtttgaa agaaagaaac ctcacg
//
LOCUS       DAOPSS010000010        21633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2032_length_21633_cov_9.558068, whole genome shotgun sequence.
ACCESSION   DAOPSS010000010 DAOPSS010000000
VERSION     DAOPSS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 21633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21633
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2032_length_21633_cov_9.558068"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..179
                     /locus_tag="PKN31_01340"
     CDS             6..179
                     /locus_tag="PKN31_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOA98605.1"
                     /translation="MRPGEALKLKKEDFNFASFEVTIPEDVAKTRKKRTLPLSPVVAK
                     EIKKLLFLQPLEC"
     gene            complement(180..317)
                     /locus_tag="PKN31_01345"
     CDS             complement(180..317)
                     /locus_tag="PKN31_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98606.1"
                     /translation="MREEASFLKTVAQKAGFWVSDKEADEKVRIAYQVLQRRALINLA
                     K"
     gene            complement(389..1216)
                     /locus_tag="PKN31_01350"
     CDS             complement(389..1216)
                     /locus_tag="PKN31_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98607.1"
                     /translation="MRKVFSKFSPRDFQFLADLVGEDKDKTYFIDLVTRDPDSLDLVL
                     EEEEIFKKVVQSEDVFVRISPYLYFELLLRQAIRDIKRDAYTLEQVGYKTRVPVFDSQ
                     KVLEVLSQDEIRDYLVELLVSFIRVNQFTIYLNRGRGIYFKKVLSDMDFDLLVEMSEI
                     VDEPYRFVFLKRAGDLALFLSGIFPEQLFGERSHSLAKNRRLVSWTNKGEDLEKKGSE
                     LYHKASESEIAKEKELSPVLTMMADNFTFTKKPLNVMADRYLYLRKHDIFPFIDNQN"
     gene            complement(1231..2061)
                     /locus_tag="PKN31_01355"
     CDS             complement(1231..2061)
                     /locus_tag="PKN31_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98608.1"
                     /translation="MSKRLVLVVIWLAILVYPVLANEDVILDYALCVGLDQDGLPLER
                     RDIFITEEEKILCWVFVSEVHKGDELRWEFQPPWGEVYESILIVEEDNRYVGATGELS
                     LAIYRNTIPPGEWKVNFYLNGEKYLELPFTLLESNIGESETLEEAIQKTVVVIRKLGY
                     EVLDLNVDDSNQAYLRMNMVAPTLDQRVWNQIGIGFESLKRVFPQSSWFLVQLILDNE
                     YVLSFQVRAYDFDMWRKGNLNTDEFWKKKVHRYVYDLKNKEEIQDVSSFYLEKFEVIY
                     "
     gene            complement(2115..2915)
                     /locus_tag="PKN31_01360"
     CDS             complement(2115..2915)
                     /locus_tag="PKN31_01360"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOA98609.1"
                     /translation="MLIVAERINATRKPVREALERKDENFFIQEAKKQEAAGAHFIDV
                     NAGTEASQEIENLPWLVELIQDEVSIPLSLDSANDEALARALRVYNKKEVMINSFTAE
                     EERIKAILPLAKDYNALVVGLAMGKGGVPQSSEDRLRLVDTLIEAVDSYKIGRDKLFV
                     DPLVVPIGTDHSQGKVFLETVKIVKEKYDEVRTICGLSNISYGMPNRRLLNRTFLILA
                     RGYGLDASIIDPLDQELMSSIYATEVLLGYDEFCMNYLSAYRSGKLEG"
     gene            complement(2890..4242)
                     /locus_tag="PKN31_01365"
     CDS             complement(2890..4242)
                     /locus_tag="PKN31_01365"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOA98610.1"
                     /translation="MALTYEEAVKYLYGLINYEKISFSYSDLKLDRMRELVNRLSNPQ
                     NDFPSILVAGTKGKGSTAYFLERLFLAWGWKCGFYTKPHLLTFRERIRLEGNLIPAQD
                     LANLVEEIKPVVEEMRNSPWGRPTYFEVSVALAFLYFSRKKVDVAVIEVGLGGRLDAT
                     NVCEPQLTVITPISFDHTDVLGDTLSAIAREKAGILRKGIKLILLPQEEEARKTILKE
                     AQEKSTPVSEVEKECRWQILSREKSGSLFSFSIGDWGEGVCSLSLLGDHQVVNFLSAL
                     LAAREWGLSFDQQKLGRALKDLYWPGRIQVLSLEPLVVFDVAHNQASFSQLIKTLQDF
                     LGMEKAVFLLGLLEGKDYIGIAQELKGFTSRVFLTLPLNPKAILPQIISPYLKREGIE
                     LEIVKDLFQARDLAFQFAQEEKLPLVTAGSFYLAPLFAQQLFLPQEEEKAYVDSSRKN
                     "
     gene            complement(4258..5100)
                     /locus_tag="PKN31_01370"
     CDS             complement(4258..5100)
                     /locus_tag="PKN31_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOA98611.1"
                     /translation="MTASEPEINNPYLPLLLPIAEIIEETRDIKTFRLRGNSIFSYLP
                     GQFAEVFVPGRGEAPFSITSSPTDGEILEFSIKRIGTVTTALHRMNQGEEIGVRGPYG
                     NGFPIEELKNHNLLFVGGGIGLAPLRSLINYVLSPQHRGEFGEIFILYGARSPEDLVF
                     KWELGKWEEREDLHLLVTVDRGDSSWRGRVGLVPQVLKEEVKIKAEEWKSIVCGPPIM
                     IKFTIKSLLEMGFSPSQIITTLEMKMKCGLGKCGRCNIGPFYVCQEGPVFSYEVLQNL
                     PEEY"
     gene            complement(5081..6073)
                     /locus_tag="PKN31_01375"
     CDS             complement(5081..6073)
                     /locus_tag="PKN31_01375"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOA98612.1"
                     /translation="MRKAMNEGLIAKEKWWEWVEDKKDRVFLTERAKSRYGKWFPREV
                     KVVKNGKEILLPPGEEVYRYFWTGEKWKIEEGEISASSAFLFSLPCEARAIIEVTDKI
                     FLNNPIDKNYWLRRKSLSLGIMACLQPEPTCFCSSVGGGPFWRDEGALFLVPREDDIY
                     IQGEGELFSFSEGVEEKIKEEIQALQKKAESVLPSLLPSYFPQELYRYFEDEEWNELS
                     WHCINCGACTFLCPTCYCFDLNVEGKLKGFQLRSWDSCMFPKFTLHASFHNPRPTGKE
                     RVRQRVMHKFSYFPEREGNFACVGCGVCVEKCPVNWDIREAIERMVEKIDRFRT"
     gene            complement(6070..7008)
                     /locus_tag="PKN31_01380"
     CDS             complement(6070..7008)
                     /locus_tag="PKN31_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOA98613.1"
                     /translation="MFEKEIRRKSEELLETKTVDMVIGYTSGSLPFQARPVLISSPED
                     TEQLVWNHFSSPNLARYLLPYRDSSKKIALLAKGCDARAVMVLIQEKQFPRERVHIIG
                     LPCPGMISKTKLEKELGEIKEEDFSFEKDTFLWQGKKWEIFPFLEDTCSRCRYPNPPL
                     YDELIGEKREPWGSSSTDYLEKMNGQSKEERWQFWEEELSRCIRCYACRNACPLCYCK
                     ECFAESDLPRWVSGAPTLSDNLFFHLGRSMHLAGRCVECGACERACPEEIKIGLLPLK
                     VEMIIKNNFSFEAGLSLEEDAPLLTYRETDPGEFIK"
     gene            complement(7001..7435)
                     /locus_tag="PKN31_01385"
     CDS             complement(7001..7435)
                     /locus_tag="PKN31_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOA98614.1"
                     /translation="MKNNWEPKIVAFLCHWCSYAGADLAGISRIQYPPNIRVIRVPCS
                     GAVNPLYILKALREGADGVLVSGCHPGDCHYLSGNYFARRKFFILHELLQWVGIEKDR
                     VQFSWVSASEGQKFSQVVEETVEKVKKLGPAKKLVKEFTFHV"
     gene            complement(7432..11829)
                     /locus_tag="PKN31_01390"
     CDS             complement(7432..11829)
                     /locus_tag="PKN31_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA98615.1"
                     /translation="MTENKVGKVLVVGGGIAGIQSALDLANSGFKVYIVESSPSIGGL
                     MARLDKTFPTNDCAMCILSPKLVECGRHFNIEILSYSEVESIEGEAGNFTVKVRRKTR
                     FVEEDKCTGCGDCEEVCPIDLPNEFEGELSNRKAIFRYFPQAFPGAFTIEKKGVPNCQ
                     ANCPLEQKAEGYIALLRKGQLEEALRAIYLDNPFPGICGRVCHHPCMEHCQRGILDEP
                     LGIPYLKRFLADYGREKGICPLPEPEEKKDKKVAIIGAGPSGLSCAYFLSWKGYEVTV
                     LEKRKKAGGMMTFGIPPYRLPREVVGEEIKVIENLGVKIIYGQEWGRDFSLSTLFEEG
                     FSAIYIATGAWKGMKLGIEGEDHPRVWDGLRYLVSLNQGDDVPEAENVVVIGGGNVAI
                     DCARSALRQGAKSVSVYYRRSREEMPARAEEIEEAQEEGVEFYFCASPRRFEERDGHL
                     FMETYVMRLCAPDESGRRRPEIIPGAGFEVEGDLFLVATGQRVDIPEGEVELTSYGTI
                     MVDDALHTSRGGVFAGGDVVLGPATLVEAIAQGKRAALSISSFLQGEKFSEEKRPPAP
                     LNIPWNRERKPQVKVKKLPAEERIRNFSEVSLGYSAEEAQSEAERCLTCGGCAECMQC
                     VLACQRGAINHKEEDREEEIQVGAIIYTTGAHAFDPSSAYRELKYKKYPNVITSLDFE
                     RILNASGPFRGKVIRPGDGKVPQKVAFIQCVGSRDPDRGKPYCSSVCCMYAIKEALVA
                     KEHLLLEGEKDFQVTIFYIDLRSYGKDFERYYERAKKEGIRFVQSKVDKVEELDNGDL
                     ELYYSNPQGGVEKEVFDLVVLSVGLEIEEKQKEELEKLGFSLSPEGFPYTDSLFPIRS
                     LKEGIWVAGTLGGPKDIPDTVIEASAASCEVASFLHSSRFQEVKEKEYPPERNVESEP
                     LRIGVFICHCGINIAGVVKIEEVVEKAKKLPGVAYVEANLYTCSQDTQERIKEKIEEY
                     HLNRIVVASCSPRTHEPLFQETLREAGLNPHLFEMANIRDQCSWVHQLQPEEATKKAQ
                     DLVAMAVAKASFLEPLQPVSLSVDKSVLIVGGGIAGLVAAKEAQRQGLRVYLVEKEKE
                     LGGQLRGFNHRFTLEGEDLVEFKNSLERDISEHPDIETFREAEVIEVNGFVGNFESKL
                     RIKGEEKVIRHGGVIIATGATEHQPREFHYGEDERIITQTELEKMLNEGLSSSPQRVV
                     MIQCVGSRDEEHPYCSKVCCFTALKNALTLKKFSPQTEVYILYRDMRSYGLAESYYRE
                     AREKGVVFIPFPDDLPPSLSLGEDIALHLRSSLGEEVILHSDLVVLSTGIEPQRSNEK
                     IAQLFKVPLNQDGFFLEAHVKLRPVDFATDGVYVCGLAHSPKPARESILQAKACVSRL
                     MNVLSKDTIQSEAQIAQVIKERCTACGDCEKVCQYQAVKVDQEQRIAEVNAALCKGCG
                     LCSATCKSGAIRVPGFAPEEIVSEMEYIL"
     gene            complement(11816..12307)
                     /locus_tag="PKN31_01395"
     CDS             complement(11816..12307)
                     /locus_tag="PKN31_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOA98616.1"
                     /translation="MVVDYQFKYQVANRPGGEGLLVCFSCGICTAGCPVAEVEEGFNP
                     RRIIHQVLLGEREGVLRSKFIWMCVGCYRCTAHCPQDVEFTNILKVLREMAAEEGYVD
                     THWIKTVEDIDRKTQKLRRDLIDYLWEEKIPSFQEYVDLYQEEVRKLSWVREQGEKEN
                     DRK"
     gene            complement(12312..13223)
                     /locus_tag="PKN31_01400"
     CDS             complement(12312..13223)
                     /locus_tag="PKN31_01400"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOA98617.1"
                     /translation="MSALQEILRRGEFAVTAEVGPPKGVNREVIEKKCDGLRGFVDAV
                     NITDNQTAIVRLSSFASCLIAKDKGIEPVMQMVCRDRNRIALQSDFLGACAWGVENFL
                     CLSGDHQSMGNHPQSKNVFDVDSIQLLQILKNMRDEKVFQNGEPLKGEIKAFLGAGAN
                     PFADPLEFRVIRLAKKISAGAQFIQTQAVFDLEIFSRFMEEVRKAGLDKKAAILAGVI
                     PVKSLSALRYMQNEVPGVVVPDSLVERMKKAKDPKEEGVRICVETIEQLKKIEGVRGV
                     HIMAISWESIVPEIVKEANLYPRPERS"
     gene            complement(13220..13870)
                     /locus_tag="PKN31_01405"
     CDS             complement(13220..13870)
                     /locus_tag="PKN31_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOA98618.1"
                     /translation="MIIAERKPIKEIFSFLVPGEKVLVLGCGTCVTVCMSGGEKEARE
                     LSSLLHLKGWEAEDSTIERQCEPEFNLKAKEEITHADTIISLACGVGVQTLAEYFPEK
                     KIFPGLNTTFMGMPKEQGLWEERCWGCGNCTVHLFGYICPVTRCAKSLLNGPCGGSKS
                     GKCEIKPDRDCAWQLIYDRLKKENKLEVLYAIQPPRDWSTSHYGGQRKMIREDVRL"
     gene            complement(13867..14229)
                     /locus_tag="PKN31_01410"
     CDS             complement(13867..14229)
                     /locus_tag="PKN31_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOA98619.1"
                     /translation="MKVVALGCDKSGYPALLRWGEKELLPFEEVEVFSIPCLGLVTEE
                     MIFQILENSDALLLAGCPLDSCHNQKGSRGAYLKTKRVNSLLQEADISKKVAIAFVTS
                     GKVGEIEKVSEEFIWREK"
     gene            complement(14226..15611)
                     /locus_tag="PKN31_01415"
     CDS             complement(14226..15611)
                     /locus_tag="PKN31_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA98620.1"
                     /translation="MSKLLWLIDDALPFPDFLIEEMERNNGWKIEVRYWENKPDSFID
                     YDRIFILGNSLSFPQERLSLLSWEWGVNPSRIQVFPGEEVRIIFPESFLSILEKFIVR
                     SLSQPLFPLEARMVVPENRVLVLPQASPALEKSLKERGIDTIIPPPSFSLRREGVNFY
                     LSPSGESVGAVIVLPSEEKKPPVSFSSQIQDTRRLVNLEFMKKQVEENRFTGETIIFI
                     LPKKGVSEEEWSQVFHLSWLLAQRDRAEVLVLVEEVLVAGEGLEKEYRIARSSGVIFE
                     KVDFSNLLTQPTLDMRGIEVELTTERDKLRQRITANWLVFLPERNILPFDLAPFFRGD
                     KIEGEILPLENPNISPFSSGIEGIFISLPGEEEKFSQVVEQYLRKGMVREIGRIEVDE
                     EKCVLCLTCLRTCPWGAIEIEEQKKKTRINWELCHLCGLCTSFCPAHAIEAKGLSLED
                     WISVISLGGKR"
     gene            complement(15614..16594)
                     /locus_tag="PKN31_01420"
     CDS             complement(15614..16594)
                     /locus_tag="PKN31_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA98621.1"
                     /translation="MTYDLVIIGGGVAGLTLAQTLQDRLKVLVIEESSLGGLSTDLGC
                     KATDECLYCGVCQFINLQKELFFKKFDVLEGRKITFLDKSGREFQIKTEKGERITSQL
                     LVVATGASPLEIKSLPLLGGGKFPRVYSGWDIERGLNEGIISSFRDFSSLAFIQCAGS
                     RNFKEKRGYCSQVCCRYALRIAENLKFSYPQLNIDFYYMDLQILGKKKGKLWEIADKV
                     NLIRHLPYEVKEREGKLSLVFEEQGVERKDYDAVILSVGMIPSSGTKEIGRWLGLNFD
                     STGFIKSYGEGHTSCKGVFVCGTACGPKDIETTREEAQMVAEEIWRVGRG"
     gene            complement(16605..18299)
                     /locus_tag="PKN31_01425"
     CDS             complement(16605..18299)
                     /locus_tag="PKN31_01425"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOA98622.1"
                     /translation="MLSDLEIAQKAQMKPITEIAQSLGLSSDDIDLYGKYKAKVSLEV
                     LENFSEKPQSKYIDVTAITPTPLGEGKTVTTIGLSQALNKIGKKSIVCIRQPSLGPVF
                     GIKGGAAGGGYSQVVPMEDFNLHLTGDTHAVAAANNLLAAFIDNHLHHGNKLNIDPFT
                     ISWPRVVDISDRALRKIVIGLGGKTNGVPRESSFDIAVASEVMAILALTTDIFDLRAR
                     LGRIVIGYNKDKKPVTAEDLQCAGAMTVLMKEAIKPNLLQTLENTPCFVHAGPFANIA
                     HGNSSIIADQIALRLSDYVVTESGFGADCGMEKFMDIKCRYSGLIPSCVVMVTSIRAL
                     KMHSGKYKVVAGRPLDPGLTSEDVEAVEKGSENMIKQIENARLFGVPVVVTINLFASD
                     TDREVETVRRIALENGAFAAEVSEVWEKGGEGGRNLAEAVVRACDEPQDFRFLYPLDI
                     PIKEKIEIIATKIYGADGVIYEPKAEEGIKRFTDLGWDKLPICMAKTHLSLSHDPKLK
                     GRPRGFKLPIRDIRPSIGAGFLYPLCGEMSTMPGLPSEPAGNKVDIDHQGRVVGLF"
     gene            complement(18421..19062)
                     /locus_tag="PKN31_01430"
     CDS             complement(18421..19062)
                     /locus_tag="PKN31_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOA98623.1"
                     /translation="MAEILDQIAKELFAGNAKAVAELTQKALSEGFSPQEILNEGMIK
                     GMNEVGVKFKANEIYVPEVLIAARAMKAGMEILKPKLAETGVEPVGKMIIGTVKGDLH
                     DIGKNLVAMMMEGAGFEVIDLGIDVPAEKFIQAIEEYQPQLVGMSALLTTTMIQIREN
                     IKAFQEASIRDQVKVMIGGAPVTQKFADEVGADGYAPDAASAVDKAKELLGIS"
     gene            19191..19967
                     /locus_tag="PKN31_01435"
     CDS             19191..19967
                     /locus_tag="PKN31_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOA98624.1"
                     /translation="MNQERTIIQRWCEEKTEKRRGIIIFIAAILAGAYIGFAAQLFTL
                     VTTSSLPLGLNQLLGGLAFSVGLIMVILGKTELFTGNCLLAYRCFDSPHRWRETLINW
                     LVVYAGNFSGALLLLLLYYLSGLFSMGNFLIAERAYAILEAKASLTFSQAFFRGILCN
                     WLVCLAVLLSLFSENNLTRLLVIPIPITTFVALGYEHSIANMYFLPAGMIAQNFIEGA
                     KNISWGAIFIKNLIPVTLGNIIGGSLLVGFLYWIMTSREE"
     gene            complement(20136..20624)
                     /gene="tsaA"
                     /locus_tag="PKN31_01440"
     CDS             complement(20136..20624)
                     /gene="tsaA"
                     /locus_tag="PKN31_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOA98625.1"
                     /translation="MMQICYQPIGIIHSPYLKPEGTPIQPRTAGGVSGTVEIFDQYVE
                     GLQDLEGFSHIILIYHFHLARPGSLKVKPFLDQKLRGVFATRAPSRPNPIGLSVVKLL
                     KVENNLLSIQELDILDGTPLLDIKPYLPDFDSPPVEKRGWLEEKLSNLPYARDDGRFA
                     ES"
     gene            20893..21525
                     /locus_tag="PKN31_01445"
     CDS             20893..21525
                     /locus_tag="PKN31_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA98626.1"
                     /translation="MSLIAFEQNKRLEKQINFIIEIDKVKNVIRKSLLFDGSRFENVA
                     EHSWTVSIMAILLKEYANFKVDIGKVLFMLLIHDIVEVDAGDTFLYSDERKNAHFNEQ
                     RAAERIFGILEPDQKKMFIDTWKEYEEGKTNEAKFALVFDRLQPITQNYVCKGITWKK
                     HNVTYEQVVEENKCIKEGSEEIWSFVEYILQKSVEKGYLAKNQPPREGGK"
BASE COUNT         6189 a         5212 c         3850 g         6382 t
ORIGIN      
        1 ctggcatccg ccccggagaa gcattaaagc taaagaaaga agactttaac tttgcctctt
       61 ttgaggtaac tattcctgaa gatgtcgcta aaacaagaaa gaaaagaact cttcctctct
      121 ctcccgtagt tgctaaagag attaaaaagc tcctctttct gcaacctctc gagtgttaac
      181 tactttgcta agttgatcag tgctcgtctt tgcagtacct gataggctat tcttactttc
      241 tcatctgctt ctttatcact cacccagaag cctgctttct gtgccactgt ttttagaaaa
      301 gaggcttctt ctctcataaa taagaagcat taccaccatt tttgtggcta actgcttgta
      361 aaaataagaa ataaggtcct atccaaaatt agttttggtt atcaataaag ggaaaaatat
      421 cgtgttttct gaggtataga tatctatctg ccataacgtt aagaggtttt ttagtgaaag
      481 taaaattatc tgccatcata gtcaatacag gactcaattc tttttcctta gctatttcgc
      541 tctcgctagc tttgtgatat agctcgcttc cttttttctc caaatcttcc cccttgttag
      601 tccaggaaac caaccgtcta tttttggcca gagaatgaga tcgctctcca aaaagttgtt
      661 caggaaatat accacttaag aataaagcca ggtctcccgc tcgtttcaga aagacaaagc
      721 gataaggctc atctactatt tcactcattt ctaccagaag gtcaaaatcc atatcggata
      781 aaactttttt gaaataaatg cctctacccc gattgagata aatggtaaat tgattaaccc
      841 ggataaaaga aactagcagc tccaccagat agtctctgat ctcgtcctgg gaaaggacct
      901 ctaaaacttt ttggctatca aaaaccggca ctcgagtttt gtaacctact tgctctagtg
      961 tatatgcatc tctttttatg tcccggatag cttgacggag aagaagttca aaatatagat
     1021 aaggactgat gcggacaaaa acatcctcac tctggactac cttcttaaaa atttcctctt
     1081 cttccagcac caaatctaaa gaatcaggat cccgggtcac cagatcgata aaataggtct
     1141 tatctttatc ctctcccact aaatcagcta aaaattgaaa gtctctcggt gaaaacttac
     1201 taaaaacttt cctcattgaa actcaccttc tcaatatatt acctcaaact tctccagata
     1261 aaaggaagat acatcctgaa tctcttcctt attctttaaa tcataaacgt aacgatgcac
     1321 ctttttcttc cagaactcat cagtattcag atttcctttt ctccacatat caaaatcata
     1381 agctcttacc tgaaaactta atacatactc attatctaag ataagctgaa ctaaaaacca
     1441 ggatgattga ggaaaaactc tttttaaaga ttcaaaccct ataccaatct ggttccacac
     1501 tcgttggtca agggtaggag ccaccatatt cattcttaaa taagcttgat tactatcatc
     1561 aacatttaaa tccaggactt cgtatcccaa ttttctgata actacaacag ttttctgaat
     1621 agcctcttct aaagtttcac tttcgccgat attgctctcc agaagagtaa aaggaagctc
     1681 cagatacttt tccccattga gataaaaatt tactttccac tctccaggag ggatggtatt
     1741 acgataaatg gcgagagaca gctccccggt agcacctaca tatcgattgt cctcttccac
     1801 tattaaaatg ctttcatata cttctcccca gggtggttga aattcccaac gcaactcatc
     1861 tcccttgtga acttcgctca caaataccca gcaaagtatc ttttcttcct cagtaataaa
     1921 tatatcccgg cgctcaagag gtagcccatc ctggtctaat cctacgcata aagcgtaatc
     1981 taaaattaca tcttcattag ccaagacagg gtagactaaa atagccagcc agataacaac
     2041 taaaactaat cttttagaca aaagactgta cctccggtct ttttactaaa ggggggataa
     2101 attttgatac cagttcaacc ttccaattta cctgaccgat aagctgataa ataattcatg
     2161 caaaactcat catagcccaa aagaacctca gtagcgtaga tgctactcat tagctcctga
     2221 tccagaggat caatgataga agcgtccaaa ccataacctc gagctagaat gagaaaggta
     2281 cggttaagca acctccgatt gggcatgcca taagagatgt tgctgaggcc acaaatagtt
     2341 ctaacttcat catatttttc ttttactatt ttcactgttt caagaaaaac cttaccctga
     2401 gagtgatcgg tgccaatagg tactaccagg gggtctacaa aaagtttatc tcttccaatc
     2461 ttataagaat ctaccgcttc tatcagcgta tccaccaatc tcaggcggtc ttctgaagac
     2521 tgagggactc ctccttttcc catagccaaa cccactacca aagcattata atctttcgct
     2581 aaaggtaaaa tagcttttat tctctcttct tcagcagtga aagaattaat cattacttct
     2641 tttttattgt aaactcgtag agcccgggct aaagcctcgt cgttggccga atcaagagaa
     2701 agaggaatgc ttacctcatc ctgaattaat tccacaagcc acggtagatt ttctatttcc
     2761 tggctggctt cagttccagc attcacatct ataaagtgag cccctgctgc ctcctgcttt
     2821 ttagcttcct gaataaaaaa attttcatcc tttctttcca gagcttctcg aacaggtttt
     2881 cgagtagcat taattctttc tgctactatc aacataagct ttttcctcct cttggggtaa
     2941 aaataattgt tgagcaaaaa gtggagcgag gtaaaaagaa cccgccgtaa ctaaaggcaa
     3001 cttctcttct tgagcaaact gaaaagccag atctcgtgct tgaaaaaggt ctttaactat
     3061 ttctaattct ataccttccc tctttaggta aggggatatt atctggggaa ggatagcttt
     3121 aggatttaaa ggtaaagtga ggaaaacgcg cgaggtaaat ccttttagct cctgggctat
     3181 acctatatag tcctttccct ctaaaagacc gagtaaaaat actgcttttt ccattcccaa
     3241 aaaatcctgg agagtcttaa tcaactgaga aaaagaagcc tgattatggg ctacatcaaa
     3301 taccaccaaa ggctccagag agagaacctg aatcctaccc ggccagtaca ggtcttttag
     3361 agctctacct aatttttgtt gatcgaaaga taagccccat tctcgagcgg cgagaagtgc
     3421 actaagaaaa ttaactacct ggtggtctcc taaaagagaa agactgcata caccttctcc
     3481 ccaatcacct atcgaaaaag aaaatagcga gccacttttc tctcgagata aaatttgcca
     3541 ccggcattct ttttctacct cactaactgg tgtacttttt tcttgtgctt ccttcaaaat
     3601 ggtttttcga gcttcttctt cttgaggtaa gagaattaat ttaatacctt ttcttaaaat
     3661 tcctgctttt tctcgggcaa tggcagaaag ggtatctccc agaacatcgg tatggtcaaa
     3721 gctgatagga gtgataacag taagctgagg ttcacaaaca ttggtagcat ctaatcttcc
     3781 tcctaacccc acttctatta ctgccacatc tactttcttc cgagaaaaat aaaggaaagc
     3841 taaagctacc gacacttcaa aataagtagg ccttccccag ggagagtttc tcatctcctc
     3901 taccaccggt tttatctcct caacaaggtt cgcgagatcc tgagcaggga tcagattacc
     3961 ctccaaccgt attctttccc gaaaagtcag aagatgaggt ttagtataaa aaccacactt
     4021 ccacccccag gctaaaaaga gccgctccag aaaataagca gtgctacctt tgcctttcgt
     4081 tcccgctaca agaatggagg gaaaatcatt ctggggatta cttaatctat ttaccagttc
     4141 tctcattcta tccagtttta aatcagaata ggaaaaactg attttttcgt aattgatcaa
     4201 accatagaga tactttaccg cttcctcgta agtcaaagcc actccttctt tccctcttca
     4261 gtattcttct ggtaaattct gcaatacttc ataagaaaaa accggtcctt cctggcaaac
     4321 gtagaaaggc ccgatattgc accggccaca tttccccaaa ccacacttca ttttcatctc
     4381 taaggtagtg attatctgag aaggggaaaa acccatctcc agaagagact taatggtaaa
     4441 ttttatcata atcggtggac cacaaactat acttttccac tcctctgcct ttatttttac
     4501 ctcctctttt agcacttgag gaaccaaccc tactcttccc ctccaggaac tatctccccg
     4561 gtctacagta acgagaagat ggaggtcttc tctttcctcc cattttccta actcccactt
     4621 aaagaccaga tcttcgggac ttcttgcccc gtaaaggatg aatatttctc caaactctcc
     4681 ccggtgctga ggagaaagaa cgtagttaat gagagaacgc aaaggagcca gcccgatccc
     4741 tcctcccaca aagagaagat tatggttttt tagctcctct atggggaagc catttccata
     4801 gggacctcgc actcctatct cttctccctg gttcatccga tgcagagcag tggttaccgt
     4861 tcctatcctt ttaatgctga attctaaaat ttctccatcg gtaggagaag aagttataga
     4921 aaagggggct tctcctctcc ccggaacaaa tacttcagca aattgcccgg gaagatagga
     4981 aaagattgag ttccctcgca agcggaaagt cttaatatct ctggtttcct cgataatttc
     5041 cgctataggt aaaagaagag gtagataagg attattaatt tcaggttctg aagcggtcaa
     5101 ttttttccac catcctctca atagcctctc gaatgtccca gtttaccggg cacttctcta
     5161 cgcacactcc acaacctacg caggcaaaat ttccttctcg ctctggaaaa taggaaaatt
     5221 tgtgcatcac tcgttgcctc actcgctctt ttcccgtagg acggggatta tgaaaagaag
     5281 cgtgaagggt aaatttggga aacatacagc tatcccagct gcggagctga aaccctttta
     5341 gtttcccttc tacgtttaaa tcaaagcaat aacaggtagg gcataaaaac gtgcaagcac
     5401 cacagtttat acaatgccag ctgagctcat tccattcttc atcttcaaaa taacggtaga
     5461 gttcttgagg aaaataagaa ggaagaagag aaggaagcac actttcggct tttttttgaa
     5521 gagcctgaat ttcctctttt attttttctt ccaccccttc actaaaggaa aaaagctctc
     5581 cttctccctg gatataaata tcatcttccc gaggaactaa aaataaagct ccttcatctc
     5641 tccagaaagg gccccctcct actgaagaac aaaaacaggt gggctcaggt tgtaaacaag
     5701 ccataattcc caaagaaagc gattttctcc tcagccagta gtttttatct atcgggttat
     5761 ttaaaaaaat tttatcagta acctcaataa tcgctcgagc ttcgcaggga agagaaaaaa
     5821 ggaaagctga agaggcacta atctctcctt cttctatttt ccacttctcc cctgtccaga
     5881 agtaacggta aacttcctcc ccgggaggga gaagaatttc ctttccattt ttaactactt
     5941 ttacttcccg gggaaaccac tttccatacc tgcttttagc ccgttcggtt aaaaatactc
     6001 tgtctttttt atcttccacc cactcccacc atttttcctt agctattaat ccttcattca
     6061 tcgctttcct cattttatga actccccggg gtcagtttct ctgtaagtta aaagaggggc
     6121 atcttcttca agtgacagcc cggcttcaaa agagaaatta ttttttatta tcatttctac
     6181 ttttaaaggt aaaagtccga tttttatctc ttccggacag gctcgctcac aagctccaca
     6241 ctccacgcac cttcctgcca gatgcatact tctacctaaa tggaaaaaca aattatcaga
     6301 aagagtggga gccccgctca cccagcgagg taaatcactt tctgcaaagc actccttaca
     6361 gtagcacagt ggacaggcat tgcggcaggc ataacagcgg atacaacggc tcaactcttc
     6421 ttcccaaaat tgccatctct cttccttcga ttggccattc attttttcta agtaatcggt
     6481 gctggaactt ccccagggtt ctcttttttc ccctattagc tcatcataaa ggggaggatt
     6541 aggatatcgg cagcgagagc aagtgtcttc taaaaaagga aatatctccc attttttacc
     6601 ttgccagaga aaagtatctt tctcaaagga gaaatcttct tcttttattt ctcccagctc
     6661 tttttctaac ttggttttgc taatcattcc cgggcacggt aatcctatta tatgaactct
     6721 ctcccggggg aattgttttt cctgaatcaa aaccattact gctcgagcat cgcacccttt
     6781 ggccagaaga gcaatctttt tagagctatc ccggtagggt aaaagatagc gagctaaatt
     6841 ggggctcgag aagtgattcc acaccagttg ctctgtatct tcaggagagg aaatcaaaac
     6901 tggtctggcc tgaaaaggta aagacccgga ggtatagcca ataaccatat ctactgtttt
     6961 tgtttccaaa agctcttcgg attttcttcg tatttctttt tcaaacatgg aaggtaaact
     7021 ccttcaccaa ctttttagct ggacccaatt ttttcacttt ttctaccgtt tcttctacca
     7081 cttgagaaaa cttttgtcct tcagaagctg aaacccagga aaactgcact cgatcttttt
     7141 ctattcctac ccattgtaat aattcatgga gaataaaaaa tttacgccgg gcaaaataat
     7201 taccactcag gtaatggcaa tcaccagggt gacaaccaga aactaaaact ccatctgccc
     7261 cttctcgaag agctttcaaa atataaagag gattaaccgc tcccgaacag ggaacacgaa
     7321 tcacccggat gttgggagga tattgaattc tgcttattcc tgctaaatca gcaccagcat
     7381 aactacacca gtggcacaga aaagctacaa ttttaggttc ccagttgttt ttcatagtat
     7441 atactccatc tccgagacta tttcttccgg agcaaatccc ggcacccgga tagctccact
     7501 tttacaggta gcactgcaaa ggccgcaccc cttgcacaaa gcggcgttaa cctcggctat
     7561 cctttgctcc tggtctactt ttactgcctg atactgacag actttttcac aatcaccaca
     7621 ggcagtgcat cgttccttaa tcacctgggc aatttgagct tcagattgaa tggtgtcttt
     7681 acttaaaaca ttcataagcc gggaaacaca agctttggct tgcaaaatac tttctcgggc
     7741 tggtttgggg ctatgagcca ggccacaaac ataaactcca tcggtggcaa agtctaccgg
     7801 gcggagcttt acatgagctt ctaaaaagaa gccatcctgg ttaagaggca ctttaaatag
     7861 ctgagctatt ttctcgttac tcctctgtgg ttctattccg gtgctcaaaa ccactaaatc
     7921 gctatgcagg ataacttctt ctcctaagga gctcctgagg tgcagagcaa tatcttcccc
     7981 cagagataga gagggaggaa ggtcatcggg aaaggggatg aaaaccactc ctttctcccg
     8041 agcttctcga taatagcttt ctgcgagacc ataacttctc atatcccgat aaagaatgta
     8101 tacctcagtc tgaggagaga attttttaag agtcagggca tttttcaaag ccgtaaaaca
     8161 acaaacctta ctgcaataag gatgctcctc atctcttgaa cctacacact gaatcataac
     8221 cactcgttga ggagacgatg atagcccctc gtttagcatt ttttccagtt cagtctgggt
     8281 aattattctc tcatcttccc cataatgaaa ttctcgaggc tgatgttctg tagcaccggt
     8341 agcgataatt acccctccgt gacggataac tttttcttcc ccttttattc gtaatttgct
     8401 ttcaaaattg cccacaaaac cattaacctc tatcacttct gcttcccgaa aggtctctat
     8461 atccgggtgt tcacttatat ctctctctaa actgttttta aattctacta aatcttcacc
     8521 ttctaaggta aatcggtgat taaaaccccg cagctgccct cccagctctt tctctttctc
     8581 tacaaggtac acccgcaagc cttgcctctg ggcttctttt gccgctacta agccggcaat
     8641 cccccctccc acaatgagaa ccgatttatc tactgacaaa gaaacaggct gcagcggctc
     8701 taaaaaggaa gctttagcta ccgccatagc tactaagtct tgcgcttttt ttgtggcttc
     8761 ctccggctgg agctgatgta cccaagaaca ttggtctcgg atgttggcca tctcaaaaag
     8821 atgaggattc agtcctgctt cgcggagagt ttcttgaaag agaggttcat gagtccgagg
     8881 ggagcaagaa gctactacta ttctattcaa atggtattcc tctatttttt cctttattct
     8941 ctcctgagta tcctgagagc aggtataaag attggcttcc acataagcta ccccgggaag
     9001 ctttttcgct ttctccacta cttcttctat ctttaccact ccagcgatgt ttattccaca
     9061 gtggcagata aatactccaa tacgtaatgg ttcagactcc acatttctct ccgggggata
     9121 ttctttttcc ttcacctcct ggaaccgaga agagtggaga aaagaagcta cctcacaaga
     9181 agcagcactg gcctctatca ctgtatcagg aatatctttt ggtccaccta atgttcctgc
     9241 tacccatatt ccttctttta aagagcgaat ggggaaaaga gagtcggtat aaggaaatcc
     9301 ttccgggctt aaagaaaagc ctaatttttc taactcttct ttttgtttct cctctatctc
     9361 taaacccacc gacagcacca ccaaatcaaa aacttctttt tctacccctc cctgggggtt
     9421 agaataataa agttctaagt ccccattatc cagctcttca actttatcta ctttgctctg
     9481 gacaaaacgg attccctctt ttttagcccg ttcataatag cgttcaaaat cttttccgta
     9541 agagcgaagg tcgatataga aaatggtaac ctgaaaatct ttctctcctt ccagcaatag
     9601 atgttcttta gctaccagag cttctttgat agcatacata cagcaaactg aggaacagta
     9661 aggttttcct ctatccgggt ctcgagaacc cacacactgg atgaaagcca ctttttgggg
     9721 aacttttcca tctcccggtc ttattacttt ccctctaaaa ggaccagagg cattgagaat
     9781 acgctcaaaa tcgaggctgg tgatcacatt gggatatttt ttatatttta gctctcggta
     9841 agctgaagag gggtcaaagg catgggctcc ggtagtgtaa attattgctc ctacctgtat
     9901 ttcttcttcc ctgtcctcct ctttatggtt tatagctcct ctctgacagg ctaaaacaca
     9961 ctgcatacac tctgcgcatc ctccacaggt aaggcacctt tctgcttcac tttgcgcttc
    10021 ttcggcgctg tagcccaagc tcacttccga gaaatttcta atcctctctt ctgcaggaag
    10081 ctttttcact tttacttgcg gctttctttc cctgttccag ggaatattca aaggtgcagg
    10141 aggtcttttt tcttctgaaa acttttcacc ctgtagaaag ctggaaatgg aaagagcggc
    10201 tctttttccc tgagcaatgg cctccactaa agtagcaggg cctaaaacca catctccccc
    10261 ggcaaaaact ccccctcgag aagtatgtag ggcatcatcg accattatcg ttccatagga
    10321 agtgagctct acttctcctt ctggtatatc tactctttgc cctgtagcta ccaggaaaag
    10381 gtctccctcc acttcaaaac cagccccggg aataatttcc ggtcttcttc ttcccgactc
    10441 atcaggagca cataacctca taacataagt ctccataaaa agatgtccgt ctctttcttc
    10501 gaatcggcgg ggactggcac aaaagtaaaa ctctacccct tcttcttgtg cttcttcaat
    10561 ttcctcagct cgggcgggca tttcttcccg agagcggcga taatacacag agacactttt
    10621 tgccccctgg cgaagagcgc tgcgagcaca atctatggcc acgttgcctc ctccaattac
    10681 caccacattt tcagcttcag gaacatcatc tccctggttg agagaaacga gataccgaag
    10741 accatcccat actcgagggt gatcctctcc ttctatccct aatttcatcc ctttccaggc
    10801 tcctgtggct atgtatatag cagagaagcc ctcctcaaat agagtagaaa gagagaaatc
    10861 cctgccccac tcctgaccat agattatttt cactcccaaa ttttcgataa cttttatctc
    10921 ttctcctacc acctctcgag gcaggcgata gggaggaatg ccaaaggtca tcatccctcc
    10981 agcttttttt cgcttttcca gcactgttac ctcgtaacct ttccagctca aaaaataggc
    11041 gcagctcaaa ccgctgggtc ctgctccaat tatggccact tttttatctt ttttctcttc
    11101 tggctcggga agtggacaga tacctttttc tcttccataa tcagctaaaa atcgcttaag
    11161 gtaaggtatc cctaagggtt catccaggat acctcgctgg cagtgctcca tacaggggtg
    11221 atggcagact cttccacaga taccgggaaa aggattatcc agatagatag ctcgtagagc
    11281 ttcctctaat tgtccttttc gtagaagagc gatgtatcct tctgcctttt gttctaaggg
    11341 acaatttgcc tgacagttgg ggactccttt tttttcaatg gtgaaagcac cagggaaagc
    11401 ctggggaaaa taacgaaata tggcttttct gttactcagc tctccttcaa actcgttggg
    11461 taaatctatg ggacaaactt cctcgcaatc gccgcaaccg gtacatttat cttcttctac
    11521 aaatcgagtc tttcttctca cttttacagt gaaatttccc gcttctccct caatggactc
    11581 tacttccgaa taggagagaa tttcaatatt gaagtgcctt ccacattcta ctaacttagg
    11641 agagagaata cacatagcac aatcattggt ggggaaagtt ttgtccaggc gggccattag
    11701 acctccaatg gaaggagaag actccacgat atacactttg aaaccagagt tagctaaatc
    11761 gagagcggac tgaatacccg ctattcctcc tcccaccacc aatactttac ccactttatt
    11821 ttcggtcatt ctctttttct ccttgttctc tcacccagga gagcttccgg acttcctcct
    11881 ggtacaaatc tacatattct tgaaaggagg gaattttctc ttcccacagg taatcaatca
    11941 aatctcggcg tagtttctgg gtctttcggt ctatatcctc taccgttttt atccagtggg
    12001 tatctacata accttcttcg gcggccattt ctcgtaaaac tttcaaaata ttcgtgaact
    12061 ctacatcttg agggcaatgg gcagtgcagc gatagcatcc cacgcacatc cagataaatt
    12121 tggaccgcaa aacaccttct ctctctccta aaagcacctg gtgaattata cgccggggat
    12181 taaaaccttc ttctacctct gctaccggac acccggcagt gcagataccg caggaaaagc
    12241 aaactaataa tccttcccct cccgggcgat ttgctacctg gtatttaaat tgataatcta
    12301 cgaccattat ctcagctcct ctccgggcga ggatacagat tggcctcttt tactatttct
    12361 ggaactatgc tttcccagga tatagccata atatgcactc ccctcactcc ctctatcttt
    12421 ttcagctgtt caatggtttc cacgcaaatt cttactcctt cctccttggg gtctttagct
    12481 tttttcattc tctctactaa agaatcggga accaccactc ccggaacttc attttgcatg
    12541 taacgcagag cagaaagaga tttaaccgga atcacccccg ctaaaatagc agctttttta
    12601 tccaggcccg cttttcttac ctcttccata aatcgggaga aaatttccag gtcaaaaact
    12661 gcctgagttt gaataaattg tgctccggcg gagatttttt tagctaagcg aatcacccgg
    12721 aattccagag ggtcagcaaa agggttggct cctgctccta aaaaagcctt tatttcccct
    12781 tttaggggtt ccccattctg gaaaactttt tcgtctctca tattttttaa aatctggaga
    12841 agctgtatag aatccacatc aaagacattt ttggactgag ggtgatttcc catactctgg
    12901 tggtctccgc tgaggcagag aaaattctcc actccccagg cacaagcacc caaaaaatca
    12961 ctttgtagag caatgcgatt tctatctctg cagaccatct gcatcacagg ctctatcccc
    13021 ttatctttag ctatcagaca actggcaaaa ctggagagac gaacaatggc agtctggtta
    13081 tcggtgatat tcaccgcatc cacaaaacca cggagcccat cacatttttt ctctattacc
    13141 tctcgattta cccctttggg aggcccaact tccgccgtaa cagcaaattc tccccgacga
    13201 aggatttctt gcaaagcgct cataaccgga catcctctcg tatcatcttt ctctgtcccc
    13261 cgtagtgaga agtagaccaa tcccgaggag gctgaatggc atagaggacc tccagtttat
    13321 tttctttctt cagccgatcg tagataagct gccaggcaca atctctatca ggttttattt
    13381 cacatttgcc acttttcgag cctccacagg gtccattcaa aagactttta gcacaacgag
    13441 taaccgggca gatatagccg aaaagatgta cggtacaatt cccacaaccc cagcatcgct
    13501 cttcccacag accctgttct ttgggcatac ccataaaggt agtattgagg cccggaaaaa
    13561 tctttttctc cggaaagtac tcagctaaag tctgcacccc cactccacag gccagagaaa
    13621 ttatcgtatc cgcgtgtgtt atttcttctt tggctttcag attaaactcc ggctcacatt
    13681 gtctctctat agtggaatct tctgcttccc atcccttgag atgcaaaaga gaagaaagct
    13741 cccgagcttc tttttctcct cccgacatgc agactgtaac gcaagtcccg caacccagaa
    13801 ctaatacttt ctctcctggc actaaaaatg aaaaaatttc ttttatcggc tttcgttcag
    13861 ctatgatcat ttttccctcc agataaactc ctcacttact ttttctattt ctccaacttt
    13921 accggaagtg acaaaggcta tggccacttt tttggaaata tcagcttcct gtaaaagaga
    13981 atttactctt tttgttttga gataagctcc tcggcttcct ttttgattgt gacagctatc
    14041 taaggggcaa ccagctaaaa gcagggcatc actattttcc agaatttgaa aaatcatttc
    14101 ctcagttacc aaacccagac aggggataga aaagacctct acctcctcaa aaggtagcaa
    14161 ctctttttct ccccatcgaa gaagagcagg gtatccactt ttatcgcaac ccaaagctac
    14221 tactttcatc tttttcctcc taaagagatc acagatatcc agtcctcaag agataaaccc
    14281 tttgcttcga tggcatgagc ggggcagaaa gaggtacaga gtccacagag gtggcagagt
    14341 tcccagttta tcctggtttt ctttttttgt tcttctattt ctatagcccc ccaggggcaa
    14401 gtgcgcaaac aagtaagaca cagaacacac ttttcctcat ctacctctat tctacctatc
    14461 tctcttacca ttccctttct caagtattgt tctactactt gagaaaactt ctcctcttct
    14521 ccgggaagag aaataaaaat cccttctatt cccgaagaaa aaggagaaat attggggttt
    14581 tcaaggggca agatttctcc ttctatttta tctccccgaa agaaaggagc cagatcaaag
    14641 ggaagaatat tcctctcagg caggaaaacc agccaattag ccgttattct ttgcctcagt
    14701 ttatctcgct cagtagtgag ctctacttct atccctcgca tgtctaaagt aggttgggta
    14761 aggagatttg aaaaatctac tttttcaaaa ataaccccgg aagagcgcgc tattcggtat
    14821 tctttttcta agccttctcc ggctactaaa acttcttcta ctaaaactaa aacctccgct
    14881 ctgtctcttt gcgctaaaag ccaggagaga tggaatacct gagaccattc ttcctcactt
    14941 acgccctttt tggggagaat gaaaatgatg gtctctcctg taaatctatt ttcttctact
    15001 tgctttttca taaactccag gttcaccaat cgccgagtat cctgaatttg tgaagaaaaa
    15061 gaaactggag gttttttttc ttcagaaggc aacactatca ctgccccgac actttccccg
    15121 gagggagaaa gataaaaatt tactccctct cgccggagag aaaaagaagg aggaggaatt
    15181 attgtatcta tacctctctc ttttaaagat ttttccagtg ctggagatgc ttgaggcaaa
    15241 actaaaactc tattttctgg tactaccatc cgtgcttcaa gaggaaaaag gggttgcgac
    15301 agagaccgaa cgataaattt ttcgaggata gagagaaaag actccgggaa aataattcta
    15361 acttcttctc cggggaaaac ttgaattctg gaaggattaa ctccccactc ccaggaaagc
    15421 aaagataacc tttcttgagg aaaggaaaga ctgttcccca ggatgaagat cctatcgtag
    15481 tcgataaaac tatcgggttt gttttcccaa tatcttacct ctatcttcca accattattt
    15541 ctctccattt cctctattag aaaatcagga aaagggagag catcatctat taaccagaga
    15601 agtttactca aagttatcct ctccccaccc gccagatttc ttctgctacc atttgtgctt
    15661 cttcccgggt ggtttcaatg tctttaggac cacaagctgt accacataca aaaactccct
    15721 tgcaagaggt gtgtccctca ccgtaagatt ttataaaacc agtagaatca aagttgagcc
    15781 ctaaccacct tcctatttct ttagtgccag aggaaggaat cattcccact gaaagaatca
    15841 cagcatcgta atcttttctt tccactccct gttcttcaaa aactaaagac aattttccct
    15901 ccctctcttt tacctcataa ggtagatgac gaattaaatt aactttatcg gctatttccc
    15961 ataattttcc ctttttcttt cccaaaattt gtaaatccat ataataaaaa tcgatgttaa
    16021 gttgaggata ggaaaatttc agattttccg ctatacgcaa agcataacga cagcaaactt
    16081 gagaacaata accccgtttc tctttgaaat ttcgagatcc agcacactgg ataaaagcta
    16141 aagacgagaa atctcgaaaa gaggaaataa taccttcgtt taagcctctt tctatatccc
    16201 agccactata aacccgggga aatttccctc cccctaaaag agggagactt ttaatctcca
    16261 gaggagaagc accagttgct actacgagca attgactggt aattctttct cctttttcgg
    16321 tcttgatttg aaactcccta ccggatttat ccagaaaagt tatttttctg ccttctaaaa
    16381 catcaaattt tttaaagaaa agctcctttt gtagattaat aaactggcaa actccacaat
    16441 aaaggcactc atcagtagcc ttacaaccta aatcagttga tagccctccc agagatgatt
    16501 cttctatcac taaaactttc aacctatcct gcaaagtttg agcaagagtt aatcccgcca
    16561 ctcctccccc aattattact aaatcgtaag tcatttttcc tcccttaaaa aagacctact
    16621 actctcccct gatggtcaat atccacttta tttcctgccg gttctgacgg caggccaggc
    16681 atagtgctca tttctccgca aagaggatag agaaatccag caccaataga ggggcgaata
    16741 tcccggatgg gaagcttaaa ccctcggggt ctacccttta acttggggtc atgagagaga
    16801 gaaagatgag tcttagccat acagatggga agtttatccc agcccagatc ggtaaatctt
    16861 ttaatccctt cttctgcttt cggctcatag attactccat cagcaccata aattttggta
    16921 gctatgattt caattttttc tttaatagga atatctaaag ggtaaagaaa gcgaaaatct
    16981 tgaggctcat cacaggctct tactactgct tctgccaggt ttcttcctcc ttctcctcct
    17041 ttttcccata cctcagatac ttccgcagca aaagctccat tttctagagc aattctcctt
    17101 actgtttcca cttctctatc ggtatcagaa gcaaaaagat tgatagtcac cactaccggt
    17161 actccaaaaa gacgagcatt ttcaatctgc tttatcatat tctccgaacc tttttctacc
    17221 gcttctacgt cttcagaagt aagccccggg tccagaggcc taccagcaac tactttatac
    17281 tttccgctgt gcattttcaa ggcccggata gaggtaacca ttaccacaca actgggaatt
    17341 aagccactgt agcgacattt gatatccata aatttttcca tcccacaatc tgcaccaaaa
    17401 ccgctctctg taaccacgta atcactcaaa cggagagcaa tctggtcagc aataatagag
    17461 ctatttccat gggcaatgtt agcaaaagga ccagcatgaa caaagcaagg agtattctcc
    17521 agagtttgca aaagattagg tttaatagct tctttcataa gaacagtcat cgctccagca
    17581 cactgtaaat cttcagcagt aactggtttt ttgtccttat tataaccgat aactattcgt
    17641 cccagtcgcg ctcgcaggtc aaaaatatca gtagtcagag ctaaaatagc cattacctca
    17701 gaagctacgg caatatcaaa gctactttct cgaggaactc cattagtttt tcctcccaaa
    17761 ccgatgacta tttttcgcaa tgcccgatca ctgatatcta ctactcgagg ccagctgata
    17821 gtaaaaggat cgatattcaa tttgttgcca tgatgaagat gattatcgat gaaagcagcc
    17881 aataagttat tagcagcagc cacagcatgc gtatcgccgg tgagatgaag attaaaatcc
    17941 tccataggga ctacctgcga gtacccacct cctgctgctc ctcctttaat cccaaaaacc
    18001 ggacctaatg atggctgacg aatacaaact atactttttt taccgatttt attaagagcc
    18061 tgggaaaggc caatagtggt tactgtttta ccttccccca gaggcgtggg agtaatagcg
    18121 gtgacgtcaa tgtatttact ctggggtttt tcagaaaaat tttctaacac ttctaaagaa
    18181 acttttgctt tatatttacc atagagatcg atatcatcag aagataatcc cagactttgt
    18241 gctatctcag taataggctt catttgtgct ttttgagcaa tttctaaatc acttaacatt
    18301 tctaccctcc taaatttaaa ggttaaatta tttattaatt aaaataatta attaagttga
    18361 taaacaacta agagaggaca ttttccctct cttagttgtt agaatagaaa gctatacagc
    18421 tcagctaata cccagtaatt ccttggcttt atccacagct gaggcagcat caggagcata
    18481 accatctgct cccacttcat cagcaaattt ttgagttacc ggagctcctc caatcataac
    18541 ttttacctga tctctgatgc ttgcttcctg aaaagcttta atgttctctc gaatttgtat
    18601 catagtagta gtcaaaagag cagacattcc taccagctgg ggctgatatt cttctatggc
    18661 ttgaataaac ttctccgccg gcacatctat acctaaatca attacctcaa aaccggctcc
    18721 ctccatcatc atggccacta aattcttacc aatatcgtga aggtctcctt ttaccgtccc
    18781 aatgatcatc ttgcccaccg gttctactcc agtttctgcc agtttgggtt tgagaatttc
    18841 catccctgct ttcatggccc gagcagcaat caaaacctct ggaacataaa tctcattggc
    18901 tttaaacttt acccccactt cgttcatgcc ttttatcata ccttcattta aaatctcttg
    18961 aggagaaaac ccctcactga gagctttttg agtaagctct gctacagctt tagcattacc
    19021 ggcaaaaagc tcttttgcta tttgatctaa aatttccgcc attttagacc ctccctctct
    19081 taattttttg ctatattagc ataagataat agaaaaatct ataactatgc tatataattt
    19141 ttccataaaa ttaattttag cttttattta atttataagg agggttaaaa atgaatcagg
    19201 aaagaacgat catccagaga tggtgtgaag aaaaaactga aaaaagaaga ggaataatta
    19261 tctttatcgc agctatattg gctggagcat atatcgggtt tgctgctcaa ctcttcaccc
    19321 tggttaccac ttcctctcta cctttagggc ttaaccagct tttaggaggg ttagcttttt
    19381 cggtgggatt gattatggta attctgggga aaacagaact ctttaccggc aattgccttt
    19441 tagcctaccg atgtttcgac tctccccatc ggtggcggga aactttaatt aactggctgg
    19501 tggtctacgc tggaaacttt tcaggagcac tattattact tcttctctat tacctatcag
    19561 ggctattctc catgggtaac tttctcatag cagaaagagc ctatgccata ctagaagcca
    19621 aagcaagtct gaccttttcc caggcttttt tccggggtat tctctgcaac tggttagtgt
    19681 gtcttgcggt tttactttcc ttattttcag aaaataacct taccagactt ttggtaatac
    19741 ccattcctat cactactttt gtggctttag ggtatgaaca cagtatcgct aatatgtatt
    19801 ttctaccggc aggaatgata gcccagaatt tcatagaagg agcaaaaaat ataagctggg
    19861 gagcgatatt tataaaaaat ctaattcctg ttaccctagg aaacatcata ggaggaagcc
    19921 tgttagtagg atttctttac tggattatga ccagtaggga agagtaaaaa agccgggcta
    19981 aaaagataaa tcggaggcct acactacaca gaaatttgga gcaatgtgta tttttttatt
    20041 tttctaaagg agtcaggtct aaggttcgaa atatctgagt attataattc tctttagcta
    20101 tattcgctaa aaaacctctg gcgggagagt aatattcagc tctcggcaaa acgaccatcg
    20161 tcacgagcat aaggtagatt ggaaagcttc tcttccagcc atcctcgttt ttccactggt
    20221 ggagaatcaa aatcaggtaa atagggctta atatctaaaa gaggagttcc atccaggata
    20281 tctaattcct ggatagaaag gagattgttc tctactttca gtagttttac caccgaaaga
    20341 ccaataggat tgggtcggga aggagccctg gtggcgaaca ctcctctcag cttctggtct
    20401 aaaaagggct taactttaag gctaccaggc ctggccagat gaaaatgata aataaggatg
    20461 atgtgagaaa acccctccag gtcttgtaat ccttccacat attggtcgaa tatttccact
    20521 gtccctgata ccccaccagc agttcggggc tgaataggag ttccctccgg ttttagataa
    20581 ggagagtgaa taattccaat tggttgataa caaatttgca tcatcttttc ccccgtctca
    20641 attttagctc caggtaatta tactctttaa agggagaaaa gataaccgat tatgggaaaa
    20701 tgataagact tgtcatataa tagatactcg aatcattaca gtttgaaaat acgagaggac
    20761 acagcgcaaa attcattaaa aaacgtattt agcggttttt caagccggag ttagaaattc
    20821 agatttctct ggtgaatgag cgtgttagac aattgcttgt ataaataaaa actgaggagt
    20881 ttgaggctgg aaatgagttt aattgctttt gagcaaaaca aaagattaga aaaacagatt
    20941 aactttataa tcgaaataga taaagttaaa aatgtcatcc gaaaatcgtt attattcgat
    21001 ggtagtcggt ttgaaaatgt ggcagaacat tcctggaccg tatctataat ggcaatttta
    21061 ttgaaagaat acgccaactt taaagtagat ataggaaaag tgctatttat gctgctaatt
    21121 cacgatatcg tggaagttga tgctggtgac acatttttat attctgacga gagaaaaaac
    21181 gctcatttca atgaacagag agccgcagaa agaatattcg ggatactgga gcctgaccag
    21241 aaaaagatgt tcattgatac ctggaaagaa tatgaagaag ggaaaacaaa cgaagctaaa
    21301 tttgctctgg tgttcgatag attacagccc attacccaga attacgtatg taaaggtatc
    21361 acctggaaaa agcataatgt tacttatgaa caagttgtag aagaaaataa atgcatcaaa
    21421 gagggctcag aggaaatatg gagctttgta gaatacattt tacagaaatc agttgaaaaa
    21481 ggttatttgg ccaaaaacca acccccgagg gagggaggga aataaatcaa attgtactgg
    21541 aaagcaatta tcgccatgca cgccgggacg tgttttccag gaatagttct ttatcttttt
    21601 gtctctttgt tttatgttta caccggagca ctg
//
LOCUS       DAOPSS010000011        20735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2165_length_20735_cov_8.210397, whole genome shotgun sequence.
ACCESSION   DAOPSS010000011 DAOPSS010000000
VERSION     DAOPSS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 20735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20735
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2165_length_20735_cov_8.210397"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..585)
                     /locus_tag="PKN31_01450"
     CDS             complement(127..585)
                     /locus_tag="PKN31_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOA98627.1"
                     /translation="MEERKEEMTAQNNVEEMTTENNKEEMIKEEEIAGEITISPDIIA
                     TLAAITTMKVPGVTSMGGLPAAPLSTLVGRKDINKGVKVELKDKTVNLEISLVVNIDS
                     SLIEVAKEVQKEVKRVIENKTGMTVNKIDISIRDVSYSEKEKEEEENTAY"
     gene            1071..1589
                     /locus_tag="PKN31_01455"
     CDS             1071..1589
                     /locus_tag="PKN31_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOA98628.1"
                     /translation="MDIMDTKEEKLIEYLESLPQLEVSEDFAQQVMHRIEGRKKRSCY
                     ADYKKWAVVSLAAVFLVLIFFSDLPLLPELEISPSLGYRKIELVFYSPSQSSRTVAVA
                     GDFSGWDYLQMEKVKEDCWRITLKVKPGKYQYGFLVDGKEWVADPNSFRQVPDGFGKF
                     NSVLVVNGESSS"
     gene            1586..2554
                     /locus_tag="PKN31_01460"
     CDS             1586..2554
                     /locus_tag="PKN31_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98629.1"
                     /translation="MKKEIFVLFLVFFLFFVGIAPLWAASDPFSLIEKSSYPEEDKEY
                     LRDQVDSLIKEAQEKNLPLSPIVSKLKEGLTKKVTPNELVKTLNHKKDSLEKAQEILQ
                     RNGVKEEDLIINLALSLELSVSPEVVEETLNKALANDGKRLQLIVDSLSSLLEMGVDP
                     ERAGEIVEKVADKNLSSREMKKMAQLLERARRAGADSEQVAKVLADALDKYDNFNLVE
                     MEVQQFIASSREKPALRSGQGVTVSSPGITSGGTPAQEGGTPLETTTTPSPTGKPPIQ
                     EGGTPLETAPSSETKPPVGEGGGTAGEQQSSSTGKPPAQEGGTPLD"
     gene            complement(2558..3616)
                     /locus_tag="PKN31_01465"
     CDS             complement(2558..3616)
                     /locus_tag="PKN31_01465"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOA98630.1"
                     /translation="MKTYRLGVIPGDGTGPEVVREGLKVLEAVSKKSGFSYTTIFYPF
                     GGEHYLKTGETLPDSAIEEFKQLDALYLGAIGHPEVKPGILERGILLKLRFSLDQYIN
                     LRPVKLYPRVWTPLKDKGPEDIDFVVVRENTEGLYVGAGGVLHRGTPHEVATQESINT
                     RRGVERCIRFAFEFTRKRNKNNKLTLCGKTNVLTYAFDLWERTFQEVAQEYPDIKTDY
                     AHVDATTMWMVKNPEWFDVIVTDNMFGDIITDLGAMIQGGMGIAAGGNINPEGVSMFE
                     PIGGSAPKYTGKNMINPLAAISALQMLLSHIGEEEAAQKVEEALIKALESGKIKSMSA
                     GQMGLTTSEVGDLIASLV"
     gene            4066..5625
                     /gene="murJ"
                     /locus_tag="PKN31_01470"
     CDS             4066..5625
                     /gene="murJ"
                     /locus_tag="PKN31_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOA98631.1"
                     /translation="MREENSLVKNTLVVGIGTFLSRISGFFREILISSSFGATWVTDA
                     FLIAYTIPNLLRRLFAEGALSTSVVPVFSRYGEEKKSSQEFISASFTGFTLIVSLVCI
                     LGMVFSPLLVHLIAVGFRGEVEKISLTTDFTVIMFPFLLLISWSALLMGFLNTFRIFS
                     WSAVAPIFFNLGAIFSIYFFKPFLGPYALAWGVILGGIGQFLFQIPPLYRAGFRLHWD
                     WKFWKNKGFRQVIGLMLPVSFSLAVSQINTIVDRVIASTCQEGAVSALYFADRLMELP
                     LGVFGVALSVAILPSLSQKFAGEEEEEWKNTLAQGMRWILFLMLPVAAFLVIFSTPLV
                     KLVYERGAFDFIASSMTAQALIFYSLGLPFFSLNLLLTRAFYSRGNTKTPVKVSLIAV
                     FSNVVLDLLLVRWMDFSGLALASSLAAFLNSFLLILCLSRVSESLEIKTLLPGKWMGK
                     ILAQTTAFIFFCHFLQGRLYSGGEGKGLLSFIGIIVLVSGFYFLLTILFQLEEGQTIV
                     YLFRNWRNNAG"
     gene            5625..7427
                     /gene="lepA"
                     /locus_tag="PKN31_01475"
     CDS             5625..7427
                     /gene="lepA"
                     /locus_tag="PKN31_01475"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOA98632.1"
                     /translation="MDRSKIRNFGIIAHIDHGKSTLADRILEICRVVPKEKMKEQFLD
                     RMDLERERGITIKAKAVRMEYFSPRDGEKYILNLIDTPGHADFSYEVSRSLAACEGVL
                     LVVDASQGVEAQTLAHARLAVSQGLVILPVINKIDLSSADPERAMREVEAILGEDHLP
                     FTLVSAKLGTGVEELIEKIVQFIPPPQGEDSPFLQALIFDSIYDSYRGVIPFIRVKEG
                     KIKKGMEIMMFSTRKSFSVSEVGVFTPERTEVDSLGPGEVGYVVANINQLEDSQVGDT
                     ITSSSHPSPSPIPGYRKIKPLVFCSFYPVSYEDHSKLKEALERLQLNDAALSFEADFS
                     EALGFGFRCGFLGLLHMDITQERMEREFGVEVVATAPHAVYHVLTKKGEVLEVRSPKD
                     FPPPSEIEEAEEPYVEMSITVPAEYLGGVMDLCQSRRGIFKDMIYLSEKFVLLKYDLP
                     LAEILLDFYDRLKAVSRGYASLDYEFAGYRPANLVRVDILVNREKVDGLSFISPEENA
                     YYRGRELISRLKELIPRQLFTISLQAAVGGKIIAREDIPALRKDVLQKCYGGDITRKR
                     KLLEKQKEGKKRMKAVGKVKIPQEAFLAVMKSEK"
     gene            7424..8569
                     /gene="hemW"
                     /locus_tag="PKN31_01480"
     CDS             7424..8569
                     /gene="hemW"
                     /locus_tag="PKN31_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOA98633.1"
                     /translation="MKKLSLYFHFPFCVKRCRYCDFVSYPRRDRHTHLKYKKAVFQEI
                     ALKVEEEGLRGSELSTVYLGGGTPSLLFSEDVEEILNYLRHFFAFSSSIEITMEVNPE
                     TVNGDKIRAFREAGINRFSVGVQSFNPHFLCFLGRSSSLEQIENVLSCMGKINCSSWS
                     LDLLYSLPFQSFLEWQREVESALVYHPPHFSIYDLTFSPRVPLYQFAKRHPGIFPSSD
                     EEALYFEWAMERLDSEGYRHYEISNFAQPGAECRHNLNYWSDGEYLGVGTSAWSYLGG
                     RRQKNTSSLKNYFTRLREGRTPLTFQEELAPHQKLTEDIMLRLRTEEGVKTSYLLAQY
                     KREEVESKLKCLEFLSKEGFIIKEEERFYLSQKGMLVANQIFQEILD"
     gene            8596..9279
                     /locus_tag="PKN31_01485"
     CDS             8596..9279
                     /locus_tag="PKN31_01485"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOA98634.1"
                     /translation="MIPIRDELPTQKLPIVTVSIIVANVVCFIYELLLGPQLETFFFQ
                     AGLVPAVLWGKVRPLSPFIHPFLTIFTSMFLHGNLFHLLSNMLYLWIFGNNVEDFLGH
                     FHFLLFYLGCGLVAGLSHALIFPFSTIPTVGASGAISGVMGGYLILFPFARVLVLVLW
                     GFFFTVVRVPAALLLGIWVFFQLIYSLTSLLSPTLGGTAWFAHLGGFAMGCLWCKIAS
                     NHYERQVYW"
     gene            9321..9755
                     /locus_tag="PKN31_01490"
     CDS             9321..9755
                     /locus_tag="PKN31_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOA98635.1"
                     /translation="MLVEEIMLPEVIMVNDFDLIADVIRFLARRKVNGAPVVDLEQRA
                     VGYFSSSVFFRQVIELAQKEVAITGNFVVAIREKIKDFSSREVSEFMSADFDYLEADE
                     GLEEAIDLLLDTDLELVPVMQGGKIVGVINHNSVLQYFMEKK"
     gene            9771..10964
                     /locus_tag="PKN31_01495"
     CDS             9771..10964
                     /locus_tag="PKN31_01495"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOA98636.1"
                     /translation="MNKKTIRDIPQEELKGKRVLVRVDFNVPLDENRQITDDTRIVES
                     LPTIKYLKERGAKVILVSHLGRPKGKFTEELKMDPVARRLEELLGEKVYKVDDCIGEK
                     VEEAVSKLKEGEILLLENIRFYPAEEANDEEFARALSRLADIYVNDAFGTAHRAHAST
                     AGVAKFLPAYAGFLMEKELEVLGEKLNNPVRPFLAILGGAKVSSKIGVIRRLLEKVDV
                     LLLGGGMAYTFVKALGYEVGNSIVEESMIGEASDVMRIARERGVRLELPEDFVVAPEA
                     KEGVEYKVVKWNQIPPDMAGFDIGPETVEKFGRYIQEARTIFWNGPLGVFEIEPFSRG
                     TVAIAKKIAQSGATSVVGGGDTIAAIKKAGVEDKITHISTGGGASLEFLEGRVLPGVE
                     VLQDK"
     gene            complement(10975..11424)
                     /locus_tag="PKN31_01500"
     CDS             complement(10975..11424)
                     /locus_tag="PKN31_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOA98637.1"
                     /translation="MVFPKFLADCMLGKLARWLRILGYDTKYSRYLDDPKIVALARFE
                     HRILLTRDKALAEGASDVAYLISSSRVEEQVQEVIKAFHLPYPTPFPRCPHCNELLFD
                     IYQEEAKREVPLFVSLTFSQFKKCPQCHRIYWPGTHWQRIIQTLSSS"
     gene            11477..13036
                     /locus_tag="PKN31_01505"
     CDS             11477..13036
                     /locus_tag="PKN31_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOA98638.1"
                     /translation="MPNTKPPYFLGIDVGTQGLRSGIFDARGNVVALSFYSYPEHHPR
                     PGWAEQDPADWWKAIQDTVRRCVIQGEVQPEEIAALSVSASSCTVLPVDQFGNPKHRA
                     IMWMDVRAFEQAERINATHNPVLRYAGGSESPEWMIPKALWLKENLPDVFANSDYLVE
                     CQNWIVFKLTDRWVTSLNNAICKWNYCPPEGGWPVSLLRELNFEELLDKWPRDLIPVG
                     ERVGELTKRAASDLGLIPGIPVIQGGIDAYAAMVGLDVVHPHRLAMVIGSSTCHLALS
                     DSPIFSTGIWGPYQGAVLPDMWILEGGQSSTGSIIKWFRDNFLQEEFREREELENIFD
                     AMDRMAAQISPGSDGLLVLDNFQGNRSPYQDPLARGAIWGLSLSHTRAHILRAIYEGT
                     AYGTFHILETLARDGFQAEEIYVSGGGARSKLWLQIHADVADIPIYLTTVEEASTLGT
                     AIYAAVGVGFYGSIPEATLKMVQISGQIYPCQQNREIYHFYYEQYVKSYWQLRDLMHR
                     VSTRLGTTPEA"
     gene            13224..14525
                     /gene="lysA"
                     /locus_tag="PKN31_01510"
     CDS             13224..14525
                     /gene="lysA"
                     /locus_tag="PKN31_01510"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOA98639.1"
                     /translation="MLGNKSINKDNHLEIAGCDVVNLAQTMGTPLYVYDEALLRSNMQ
                     EYEKSFRENYPHFSLAYAGKAFLCSALCSIIKEEDWWLDVVSGGEYYLASTANFPVEK
                     IIFHGNNKTNEERRLVLREGVGRWVVDNEVELNLLLEEASSYASYKVPLFFRLTPGVD
                     PHTHQYITTGKIDSKFGFLLGEAENAILKAIQSEDLEVWGLHCHIGSQIDHIEPFLKA
                     AEVMLQFMVDFRKKHGFVFRELDLGGGLGVPYTDEEKSRFPSSHFYVKEISEKVKEMC
                     GELSYPLPRLYIEPGRSVVNTAGSTIYRVGSVKEIPGIKKYVAVDGGMTDNPRPVLYG
                     AKYQAHIGNRVAGEREKVSVVGKCCESGDVLIEEIELPSPSPGDVLVVEATGAYNYSM
                     ASNYNLIPRPGVVFIREGEPELVVRPENWYDLVRRDITPLE"
     gene            14544..15347
                     /gene="dapB"
                     /locus_tag="PKN31_01515"
     CDS             14544..15347
                     /gene="dapB"
                     /locus_tag="PKN31_01515"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOA98640.1"
                     /translation="MKEIGVFLVGAAGKMGKEIVRAIHKEENIVLRGAADKQEVGRDT
                     GEVSGVGPQGIIIEENLAEAINRVKPDVICDFTHASALRENLPLFLDYKIPLVIGTTG
                     LSSEEIETLRQSCEREGIGCVIAPNFALGAVLMMHFSRIAARYLERAEIIEYHHPQKK
                     DAPSGTAIKTAQGIEEVKNTPFSDKSEEILSGARGALLGSVNIHSVRLPGLVAHQEVI
                     WGGEGEILTIRHDSLSRSSFMPGVMMSIKKVYEEPRFYYGLEEVIGLTS"
     gene            15387..16241
                     /gene="dapF"
                     /locus_tag="PKN31_01520"
     CDS             15387..16241
                     /gene="dapF"
                     /locus_tag="PKN31_01520"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOA98641.1"
                     /translation="MQFVKMHGLGNDFIIFPEEETGGVSSWPSFSLKICDRHKGVGGD
                     GVILVLSSSVADLRMRIFNSDGSEAEMCGNGIRCLAKFAFERGIVNKKEFTVETKAGI
                     IIPRITEEEEGKVRRVRVNMGKPRFQPREIPVDLEGKEVKNYPLEVEGRTFLISALSL
                     GNPHVVIFEIPRDWEKYGREIENHPLFPEKTNVEFVEVRNKKEAVVKVWERGAGVTLA
                     CGTGAAAVLVASVREGKLDRKAAIHLPGGTLDVEWQEEVYIEGPAEEVFSGEINEEVE
                     KEWKLLSV"
     gene            16220..17383
                     /locus_tag="PKN31_01525"
     CDS             16220..17383
                     /locus_tag="PKN31_01525"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOA98642.1"
                     /translation="MEVAQRIKNLPPYLFAEIEKKVNQARAEGKEIIDLGIGDPDIPT
                     PSFIVERLCEEAWRPENHRYPSYQGLDAFREAVVLWYKKRFGVELDPEQEVVSLIGSK
                     EGIAHLPWCLVNPGDLVLASDPGYPVYKIGTMLAGGIPLELPLLAENDFLPDLYSFPD
                     SIWEKVKIVFLNYPNNPTASIAPLSFFEEIVELARRYDFVIAHDLAYSEISYDGYRAP
                     SILEVEGAREVAVEFHSLSKTFNMTGWRIGFAVGNEKVIEALGRVKTNIDSGIFNAIQ
                     WAGVEALNRVDEVLKNIIPVYTERRNLVVETFSSLGFDITPPQASFYLWVPVPPGFTS
                     LEFTNYLLDKSQVVVTPGTGFGRYGEGFVRISITTPSPVLEKAMMRIKETWDR"
     gene            17384..18616
                     /gene="dapG"
                     /locus_tag="PKN31_01530"
     CDS             17384..18616
                     /gene="dapG"
                     /locus_tag="PKN31_01530"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOA98643.1"
                     /translation="MTVKIVVQKFGGTSVNTRELREKAALKVQETQKEGFSPVVVVSA
                     MGRKGAPYATDTLISLAREEFSNLDPRDLDLLLSCGEIISAVVMSQTLRRMGMKSVAF
                     SGAQAGIITDNHFGDASILRVEPDRIKKYLDKGYVVVVAGFQGATEEGEVTTLGRGGS
                     DTTAVVLGIYLQADYVDIFTDVNGVFTADPRIVPEAQILQGLTYTEAAEMIQQGAKVI
                     HHKAMNLAREYRIPLRVRSLSDEGKGTLICDISTLTKEGLRILSQEEPVYSIAYRRGL
                     AQVRVNLGDSENGELKIFQSLAQEGISIDLVSLFPEGTAFVVDETVGSKVEEILRREG
                     FEVNLTLPCAKVSLVGSDMADRPGVVAQLVEALKDANIEILQTGDSHITISCLVKSQD
                     MERAIQVLHHKFRLEKAC"
     gene            18633..19514
                     /gene="dapA"
                     /locus_tag="PKN31_01535"
     CDS             18633..19514
                     /gene="dapA"
                     /locus_tag="PKN31_01535"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOA98644.1"
                     /translation="MGYFGSLLTAAVTPFDQDLTVNHFEFRKLLRALVENGSDGVVVS
                     GTTGESPTLSREEKLCLFEVALEEVGDQAKVIAGTCNYNTKESVLLSKEAESLGVHGI
                     LAVAPYYNKPPQEGLYQHFRAIAEAVNIPVMLYNIPSRTGVNIEPETIARLSEIPNIV
                     ALKEAGGSTDQISRIKALVPPDFYLYSGDDIMTLPLLALGGVGVVSVASHLVGKEIKK
                     MIQLFDEGKTKEALKVHLELYPLFKALFISTNPIPVKAALNLAGWRVGGVRPPLSSLE
                     QEKEEKLKSVLEKFHLI"
     gene            19800..>20735
                     /gene="argC"
                     /locus_tag="PKN31_01540"
     CDS             19800..>20735
                     /gene="argC"
                     /locus_tag="PKN31_01540"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOA98645.1"
                     /translation="MMVRVGVMGATGYTGLELLRILAYHPGVEVCWVGSESFPGIKLE
                     DYCLAWQGRSNLTLQKQEVDSLEGIEAIFLALPSGVAMDYVPPLLQKGIRVIDLGADF
                     RFKNVEVFQKYYQLEHRVPHLLQEAVYGIPEICRERLKEAQLVANPGCYPTSVIIPLY
                     PFLKEGVISPSIIVDSKSGVTGAGRKPTEDNQFCEVDENFKAYKVGEHRHQPEMKEQL
                     ELATGREVNLIFTPHLLPLKRGILTTVYLTLEKDIEEEELLSIWKDYWGRKPWVRIFP
                     LHRFPELRWVVGSNFVDLGLKKVGENQLIVISALDN"
BASE COUNT         5718 a         3729 c         4985 g         6303 t
ORIGIN      
        1 atacaaaaaa agataacatc ctgcagtaac agcatacaaa atcactccga gattgcgctg
       61 tggtcatagg gatgagcttt ccaaaaagag taggctatag tgatttttat gattaactat
      121 gcttccctaa taagctgtgt tttcttcctc ttccttttcc ttttcggaat aagaaacatc
      181 tctaatgctt atatctattt tattaaccgt catcccggtt ttattttcga ttaccctttt
      241 tacttctttt tgtacttctt tcgctacttc aattaaagag gagtcaatat taaccactaa
      301 agatatttct aaatttaccg ttttatcttt tagctctact ttcacccctt tgttaatatc
      361 ttttctacct actagagtgc ttaagggagc agcaggtaat ccccccatgc tggttacccc
      421 gggaactttc atagtggtga tagctgccag agtagctata atatctggag aaatagtaat
      481 ttctcccgct atttcttcct ctttgatcat ctcttctttg ttattttccg ttgtcatttc
      541 ttctacatta ttctgtgctg tcatctcttc ttttctttct tccatagatt taacacctcc
      601 ttttttatag gacctacaca cttccattac attttacaac aaaatgataa aaaatagaac
      661 tgtaaataac gtttacttct ctcttcgact aaaaaataca tattaaagac ctttataaca
      721 ggaaagactt tatactactt gtaaatataa tcacgcaaaa gattaggatt ggaaaagggt
      781 ttttaagacg gatttaaata aatgattttc tacagcaaac taatttatac gagatatagc
      841 tcttatgagg gcagcgataa ccttaaaaga agaaatggtt tagcgatgaa tttactggtt
      901 ttttgaaaaa agtaattggg gagtaatatt gtgagctcat gaggtgctgc atgaatgctt
      961 tatgggtggt cacactatat gttgtggagg gagcaatata ttgaacaaga taaacttatc
     1021 ttttgaaact gaaaccccca ggtttacgta taaagaatca ggtgaagagt atggatataa
     1081 tggatacaaa agaagagaaa cttattgaat atctggaaag cttacctcag cttgaggtaa
     1141 gcgaagattt tgctcagcaa gttatgcacc gaatagaagg gcgcaaaaag cgttcgtgtt
     1201 atgctgatta taaaaaatgg gcagtggtct ctttagcggc tgtgtttctc gttttgattt
     1261 tctttagcga tcttcctttg cttcctgagc tggagatttc tccttctcta ggttatcgga
     1321 aaatagagtt ggttttttat tctccttctc aaagctcccg aacagtagca gtagcaggag
     1381 attttagcgg ttgggactac ctgcaaatgg agaaagttaa agaagattgt tggagaatca
     1441 cgttaaaggt taaaccaggt aaatatcagt atggtttttt agtagatggt aaagaatggg
     1501 tagctgaccc caattctttc cgacaggttc ccgatggatt tgggaaattt aactcggtgc
     1561 tggtggttaa cggtgaatcg agctcatgaa gaaagagatt tttgttttat tccttgtctt
     1621 tttcttgttt tttgtgggaa tagctcccct atgggcggca agcgaccctt tttctctcat
     1681 tgaaaaatcc tcttatccag aagaggataa ggagtatctt cgggaccagg tagattcttt
     1741 aataaaagaa gcacaggaaa aaaatcttcc tctctctccc attgtttcca aactcaaaga
     1801 aggtttaacc aagaaagtca ctcctaatga actggtgaaa actttgaatc ataaaaagga
     1861 ttctctggag aaagctcagg aaattttgca aaggaatggg gtgaaagaag aagatttaat
     1921 aatcaatttg gctttatctc tagagttatc cgtttctcct gaggtagtag aagaaacttt
     1981 aaacaaagcc ttggctaatg atggaaagcg tttgcagttg atagtggatt ccctgtcttc
     2041 tcttttagag atgggggtgg atccggagag agctggggag atcgtagaaa aggtggcaga
     2101 taagaatctt tcctctcgag agatgaaaaa gatggctcaa ctcctcgaga gggcccgcag
     2161 agcaggagca gattctgaac aagttgccaa agtattagct gatgctctgg ataaatatga
     2221 caattttaat cttgtagaga tggaagtcca acagtttatt gcttcttctc gggaaaaacc
     2281 agctcttcgg tcgggacaag gggtgacagt ttcttcaccg ggaataacct ctggaggcac
     2341 tccagcacaa gaaggaggaa cacctctgga aactactact accccatctc ctacgggtaa
     2401 acctcctatc caggaaggag gaacgccttt ggaaaccgct ccttcctctg agacaaaacc
     2461 cccggtagga gagggaggag gaacagctgg agagcagcag tcctcctcta caggtaaacc
     2521 tccggcacaa gaaggaggaa cacctttaga ctgatcccta aactagggag gctattaaat
     2581 cccctacttc agaagtggtg agtcccattt gcccggcact catacttttg atttttccac
     2641 tttctaaagc tttaatcagg gcttcctcta ctttttgagc cgcttcttct tctcctatat
     2701 gagatagtag catttgcaaa gcagaaatgg ctgccaatgg attgatcata tttttccccg
     2761 tgtatttagg ggcggaaccc ccgataggct caaacataga aactccctcg gggttgatgt
     2821 tgccaccggc tgctattccc atacctcctt gaatcatagc tcctaaatca gtaatgatgt
     2881 cgccgaacat gttatcggtt actattacat cgaaccactc ggggtttttg accatccaca
     2941 tggtagtagc atctacatga gcatagtcag ttttaatatc gggatattcc tgagctactt
     3001 cttgaaaagt tctttcccat aagtcaaagg cataagttaa aacgttggtt ttaccgcaca
     3061 gggtgagttt attattttta tttctttttc tggtaaattc aaaagcaaaa cgaatgcatc
     3121 gttctactcc ccgacgagtg ttgatagatt cctgggttgc tacttcgtga ggggtacctc
     3181 gatgcaagac tcctcctgct cctacgtata agccctcggt attttctctc accaccacga
     3241 aatctatgtc ttctggccct ttatctttga ggggggtcca cactctggga taaagtttta
     3301 ctggtcggag attaatgtac tggtctaaag aaaagcgcag tttcagtaaa attcctcgct
     3361 ccagaattcc tggctttact tcaggatgcc ctatggctcc cagataaaga gcgtctaatt
     3421 gcttgaattc ttcaatagcc gaatcgggta aagtttctcc tgttttaaga taatgttctc
     3481 ctccaaaggg ataaaaaata gtagtgtaag aaaaacctga ctttttagaa acagcttcta
     3541 aaaccttgag tccttctctt actacttctg gtcctgtccc atctccgggt atcacgccca
     3601 ggcgataagt tttcattttt ttctcctttc tgttgttttt ggtctatatt ttatcatttt
     3661 ccttttactt ttgctgactt cgtaactgtc atcaaacatt tcttacccat ctttagcaat
     3721 tatgctgatg aactgtcatt cttcctgaaa aattaatatt tcaaattgcc gagtgcgcat
     3781 agtacagttt tttgggatta tgaggggggt ttccccctct cgacaaaaat tgaataatga
     3841 gttaggctat attggattga cttttcgata taataccttg ctcagaaaag cgagcgatgt
     3901 ccacctttct ttatattttc tactatagag gagaagagtt aaaattgaat atttgtcgaa
     3961 gagagaaaaa gagaaaagct gattatatag cgaagaaaaa cattttccgc tctgttctcc
     4021 tttttggcaa atagcctctt cttccttgcc aggataaaat atgagatgag agaagaaaac
     4081 tccttagtaa aaaatacctt agtagtggga attggcacct ttctttcccg gatctcgggt
     4141 ttttttaggg aaattttaat ttcctcttcc tttggggcta cctgggttac cgatgccttt
     4201 ctaattgcct ataccattcc caacctttta cgccgacttt ttgcagaagg agcactgagt
     4261 acctcagtag ttccagtttt ttctcgctat ggagaggaaa aaaagtcctc tcaagaattt
     4321 atttctgctt cttttactgg ctttacttta attgtttcat tggtttgtat tctgggaatg
     4381 gttttttccc cactgctcgt gcatctcata gcagtgggat ttcgaggtga ggtagaaaag
     4441 atatctttaa ctaccgattt taccgttatt atgtttcctt ttcttctttt gatttcctgg
     4501 tctgctttgc ttatgggttt tcttaatact tttcgtattt tcagctggtc agcagtggca
     4561 ccgattttct ttaatctggg agctattttc tctatttact tctttaagcc ttttttaggc
     4621 ccttatgcct tagcctgggg ggtaattttg ggagggattg gtcaatttct attccagatt
     4681 ccccctcttt atcgagcggg tttccgtctc cactgggatt ggaagttctg gaaaaacaaa
     4741 ggattccgac aagttattgg tctcatgctt ccagttagct tttctctggc agtatcccag
     4801 atcaatacca tagtagatag agttattgct tccacctgcc aggaaggagc agtatctgct
     4861 ctctattttg ctgatagatt aatggagcta cctctgggag tctttggagt ggcactttcc
     4921 gttgccattt taccgtctct ttctcaaaag tttgctgggg aagaggaaga agaatggaaa
     4981 aatactttag ctcaggggat gcggtggatt ttatttttga tgttgcccgt agctgctttc
     5041 ctggtaattt tttctacccc ccttgtgaaa ttggtttatg aaagaggggc atttgatttt
     5101 atagcctctt ctatgactgc tcaggcctta attttttatt ctctggggtt gccctttttt
     5161 tctttaaatc ttctccttac ccgggccttt tattctcgag gaaataccaa aaccccggtt
     5221 aaggttagtt tgattgcagt attttctaac gtagttttgg accttctcct ggttcgatgg
     5281 atggatttca gtggtctggc tttagccagt tctctggctg cctttttgaa ttcttttttg
     5341 cttattttat gtctgagtcg ggtgtctgag tcgctggaaa ttaaaactct tttacctgga
     5401 aaatggatgg ggaaaatttt agctcagacc acagctttta tttttttctg ccactttctc
     5461 cagggaaggc tttattctgg aggagaaggt aaaggtttgc tttcctttat tggtataata
     5521 gttttagtta gtggatttta ttttttgctt accattcttt ttcaactgga ggagggacaa
     5581 actattgttt atttgtttcg caactggagg aataatgctg gctaatggat agaagtaaaa
     5641 ttagaaactt tggtatcatt gctcacattg accatggcaa atcaactctc gctgatagga
     5701 ttctggaaat ctgcagggtg gtgcccaagg aaaaaatgaa agagcaattt ctggaccgta
     5761 tggatttaga aagagaaaga ggcattacca ttaaggccaa agcagtgagg atggagtatt
     5821 tttcccctcg ggacggggaa aaatacatct taaatttaat tgataccccc gggcatgccg
     5881 attttagcta tgaagtttcg cgaagcttag cagcttgtga gggagtttta ttagtggtag
     5941 atgcctctca aggggtagag gctcagactt tagctcatgc ccgtcttgct gtttctcaag
     6001 gtcttgttat tctcccggta atcaataaaa tcgatctttc ttctgctgat ccagaaaggg
     6061 ccatgaggga agtggaagct attttgggag aagaccatct tccctttact ctggtgagtg
     6121 ccaaattggg aactggagtg gaagaactaa tagaaaagat tgttcaattc attcctcccc
     6181 ctcaagggga agactctcct tttcttcaag cattaatttt tgattcgatt tacgattcct
     6241 accggggggt tattcctttc attcgggtaa aagaagggaa aattaaaaaa gggatggaaa
     6301 tcatgatgtt ttccactcgc aaaagctttt ctgtttcaga ggtaggagtt tttactcccg
     6361 aaagaacaga agtagatagt ctgggtcctg gagaggtagg ttatgtggtt gctaatatta
     6421 accagttgga agatagtcag gtgggagata ccattacttc ttcctcgcac ccctctcctt
     6481 ctcccattcc aggatacaga aaaataaaac ctctggtttt ttgtagtttt tatccagtga
     6541 gctatgaaga tcattccaaa ttaaaagaag ctttagaaag attgcagctt aacgatgctg
     6601 ctctaagctt cgaagctgat ttttcagaag ctctgggttt tggtttccgt tgcggctttt
     6661 tgggtctcct gcatatggac ataacccagg aaagaatgga gcgagagttc ggagtggaag
     6721 tggtagctac tgctccccac gcagtatatc atgttttgac taaaaaagga gaggtgctag
     6781 aggtaaggtc tcccaaggat tttcctcctc cttcagaaat agaagaagcg gaagaacctt
     6841 atgtagagat gagtataacc gttccagcag aatacttagg gggagtaatg gacctctgtc
     6901 aatctcggcg ggggattttt aaagacatga tttatttaag tgagaagttt gttcttttga
     6961 aatatgacct gcctctcgcg gaaattttac tcgatttcta tgaccgtcta aaagcggtga
     7021 gcagaggcta tgccagtttg gactatgagt ttgcagggta tcgcccggca aacctagtaa
     7081 gagtggatat tctagttaat cgagaaaaag tggatggcct ttcttttatt tctccagaag
     7141 agaatgctta ttaccggggt agagagttga tttccagatt gaaggaactt attccccggc
     7201 aactttttac catttctttg caagcagctg tggggggaaa gataattgct cgagaagata
     7261 taccagcctt gcgcaaagat gtgttacaaa agtgttacgg tggagacata accagaaaga
     7321 ggaaactcct ggaaaagcaa aaagagggca aaaagagaat gaaagcggtg ggcaaagtta
     7381 aaattcctca agaagctttt ctggcagtga tgaaaagcga gaaatgaaaa agctatctct
     7441 ttattttcat tttccttttt gtgttaaaag atgtcgttac tgcgactttg tttcttatcc
     7501 tcgaagggac cgacatactc atcttaaata caaaaaagca gtttttcaag aaatagcttt
     7561 gaaggtagaa gaggaaggcc tccggggaag tgagttgtct actgtttatc tgggaggagg
     7621 aactccttct ttgttatttt ctgaagatgt ggaagaaata cttaattatc tccggcattt
     7681 ctttgctttc tcgtcttcta tagaaataac tatggaggtg aacccggaaa cggtaaatgg
     7741 agacaaaata agagcttttc gagaagccgg aatcaaccga ttcagtgtag gagttcaatc
     7801 ttttaaccct cattttttat gctttttagg tcgttcttcc tccctggaac aaattgagaa
     7861 tgttttgtcc tgtatgggaa aaattaattg ctcctcctgg agtttagacc ttctctatag
     7921 cttacctttc cagagttttc tggagtggca aagggaagta gagagcgctc ttgtctatca
     7981 tcctcctcat ttttccatct atgatcttac cttttctccc cgggttcctc tttatcaatt
     8041 tgctaagcgc caccccggga tttttccttc ttctgatgaa gaagctctct attttgaatg
     8101 ggcaatggaa agattagaca gcgaaggata tagacattat gagatttcta attttgctca
     8161 acccggagcg gagtgccgcc ataatctcaa ttactggagt gatggagaat acttaggggt
     8221 agggacttcg gcgtggtctt acctgggagg gagaagacaa aaaaatacca gtagcttaaa
     8281 gaactacttt acgcgcttga gggagggaag aactcctctt acttttcaag aagaacttgc
     8341 ccctcaccaa aaattaacag aagatattat gctccggtta aggactgaag agggagtaaa
     8401 aacttcttat cttttagcgc aatacaaaag agaggaagta gaaagcaagt taaaatgttt
     8461 ggaatttttg tctaaagaag gctttataat taaagaggaa gaaaggtttt atctttctca
     8521 aaaaggaatg ctggtggcta atcaaatatt tcaagaaatt cttgattgag gagggtagtt
     8581 gtgttattgt aaattatgat accaattaga gatgagttac caacccagaa gcttcctata
     8641 gtaactgtat ctatcattgt agctaatgtg gtatgtttta tttacgaatt actcttaggt
     8701 cctcagctgg aaactttctt tttccaggcg ggattggttc cggcagtttt atggggtaaa
     8761 gttcgtcctt taagtccttt tatccaccct tttctcacta tttttacttc tatgtttctt
     8821 catggtaatc tcttccatct tctgtctaac atgctctatc tctggatttt tggcaacaat
     8881 gtggaagatt ttttaggcca ttttcatttt ttactattct atttgggctg tggtctcgtg
     8941 gcaggcctct ctcatgctct gattttccct ttctctacta ttcctacagt gggagcaagt
     9001 ggtgctataa gtggagtgat gggtggatat ttaatacttt ttccctttgc tcgggtgctg
     9061 gttttggtgc tatgggggtt tttctttact gtggttcgag ttccggcggc tctgcttctg
     9121 ggcatctggg tttttttcca attaatttat agtttgactt ctcttctttc tcccactttg
     9181 gggggaacag cgtggtttgc tcatctgggg gggtttgcta tgggttgctt atggtgtaaa
     9241 atagcttcca atcactatga acgtcaggtc tattggtgat aactatagag agtaataaat
     9301 cgatagggag gaatggttaa gtgttggtag aagaaattat gttacctgag gttattatgg
     9361 taaacgactt tgatctgatt gctgatgtca taagattttt ggcccggaga aaagttaatg
     9421 gagcacctgt tgtagacttg gaacaaagag ccgtgggcta cttttcttct tccgtttttt
     9481 tccggcaggt tatagaatta gctcaaaaag aagtagctat tactggtaac tttgtagtcg
     9541 ccattagaga aaaaatcaag gatttttcct ctcgggaagt ttccgaattt atgagtgctg
     9601 attttgatta tctggaagca gacgaagggt tggaagaagc tatagattta ctgcttgata
     9661 ctgatttgga acttgtgccc gtgatgcagg gagggaaaat tgtaggggtg ataaatcaca
     9721 atagcgtctt gcaatatttt atggaaaaaa aataagggag gtgtaggaag atgaataaga
     9781 aaaccattcg agatattcct caagaagagc ttaaaggaaa aagagtactg gtgagagtgg
     9841 attttaatgt tcctctggat gaaaaccggc agattaccga tgatacgagg attgtagaaa
     9901 gtttgcctac tataaagtat cttaaagaaa gaggagctaa agttattttg gtttctcatc
     9961 tgggccgccc taaaggcaaa ttcactgaag aacttaaaat ggatcctgtg gcccggcggc
    10021 tggaagaact cttaggggaa aaagtttata aagtagatga ttgtatagga gaaaaagtgg
    10081 aagaggcggt ttctaagcta aaagaagggg aaatacttct tctcgaaaat atccgcttct
    10141 atcccgcaga agaagccaac gatgaagagt ttgctcgagc tctttctcgc ttggctgata
    10201 tttacgttaa tgatgctttt ggaactgctc accgagccca tgcttccact gccggagtgg
    10261 ctaagttttt gcctgcctat gccgggtttt tgatggagaa agagctggaa gttctggggg
    10321 aaaaactcaa taatccggtg cgaccgttct tggctatcct aggaggagcc aaagtgtcaa
    10381 gtaaaattgg agtgattaga aggcttctgg aaaaagttga cgttcttctt ttgggaggag
    10441 ggatggctta tacttttgtt aaagctttag gctatgaagt gggtaattct attgtggaag
    10501 aatctatgat aggagaagcc tctgatgtta tgcggatagc tcgagaaaga ggagtgcgtc
    10561 tggagcttcc agaagatttt gtagtggctc cggaagctaa agaaggggta gagtataagg
    10621 tggtaaagtg gaaccagatc cctcctgata tggcagggtt tgacattggt ccagaaacgg
    10681 tggagaaatt tggtcgttac attcaggaag ctcgaactat tttctggaat ggacctctcg
    10741 gggtttttga gatagagcct ttttctcgag ggacagtggc catagccaaa aagattgccc
    10801 aaagcggtgc tacgagtgta gtaggcggtg gagatactat agctgctatt aagaaggcag
    10861 gggtagaaga taaaattact catatttcta ccggtggcgg tgcttcttta gaatttttag
    10921 aagggcgagt tttacccgga gtggaagtat tacaggataa atagttttgg gaggtcagga
    10981 gctcgagaga gtttggatta ttctctgcca gtgagttcct ggccaatata ttcgatgaca
    11041 ttgaggacat tttttgaatt gagaaaaggt taaagaaacg aaaagaggga cttctctctt
    11101 agcttcttcc tggtagatgt caaagagtag ttcattgcag tgggggcaac ggggaaaggg
    11161 agtaggataa ggaagatgaa aagcttttat gacttcttga acttgttctt ctacccggga
    11221 agaagagatg aggtaagcaa catcagaagc accttctgct aaagctttgt ctctggtgag
    11281 aagaattcga tgttcaaaac gggctaatgc tactattttg gggtcgtcca gatatcggga
    11341 gtacttggta tcgtatccta aaattctcag ccatcgagct aacttaccta acatgcaatc
    11401 agctaaaaat ttggggaaaa ccatgttatt attttaacat gataaagtaa tttgctgttg
    11461 tggaggtctg aaaattatgc cgaataccaa accaccttat tttttaggta ttgatgtggg
    11521 aactcaagga ttgcgaagtg gtatctttga tgctcgtggt aatgtggtag ctctttcttt
    11581 ctattcttat cctgaacatc atcctcgtcc gggatgggca gaacaagatc ctgctgattg
    11641 gtggaaagcg attcaagata ctgttcgtcg ctgcgttata cagggagaag tgcaaccgga
    11701 agagattgcc gccttgagcg tgagtgctag ttcttgcacc gtgcttcctg tagaccaatt
    11761 tggtaatcct aaacaccggg ccattatgtg gatggatgtc cgggcttttg agcaagcaga
    11821 gagaattaat gctactcata accctgttct tcgctatgca ggagggagtg aatcgccgga
    11881 gtggatgatt ccaaaggctt tgtggcttaa ggagaattta cctgatgttt ttgctaactc
    11941 tgactatttg gtagaatgcc agaattggat agtttttaaa cttactgatc gctgggtaac
    12001 ttccttgaat aatgctattt gtaaatggaa ttattgtccc cctgaaggag ggtggccagt
    12061 ttctttattg cgagaactta actttgaaga attgctggat aagtggcccc gggatttaat
    12121 cccggtaggg gaaagagtag gggagctaac caaaagagca gcttctgatt taggacttat
    12181 cccgggtatc cctgttattc aaggagggat agatgcttat gccgctatgg taggattaga
    12241 tgtagttcat cctcatagat tagccatggt aattggttct tctacctgtc atctggctct
    12301 gtctgatagt cctattttca gtaccggtat ctggggacct taccagggag cagtactacc
    12361 tgatatgtgg attttagagg gaggacagag ctcaaccggc tctatcatca aatggtttag
    12421 ggataatttt ctgcaggagg agttcagaga aagagaagaa ttggagaaca tttttgatgc
    12481 tatggatagg atggcggctc aaatctctcc cggttctgat ggtcttttag tgttagataa
    12541 tttccagggt aaccgtagtc cttatcagga tcctctggct cgaggtgcca tttgggggct
    12601 ttctctgagt catacccgag ctcatatttt gcgggctata tacgaaggca cagcttacgg
    12661 aacatttcat attctggaaa ctttagcccg ggatggtttt caggcggagg agatatatgt
    12721 aagtggaggg ggagcgagga gtaaactgtg gttacaaatt catgctgatg tggctgatat
    12781 tcctatttac ctgaccactg tggaagaagc cagtacctta ggaacggcaa tttatgccgc
    12841 tgtgggagtg ggtttttatg gaagtattcc cgaagctacc ctcaaaatgg tgcaaatatc
    12901 aggtcaaata tatccttgcc aacagaacag ggagatttat catttttatt atgagcaata
    12961 tgttaaaagt tactggcaac tccgagatct gatgcatcgg gtttctacta ggctgggaac
    13021 cactccagag gcttaattca cccccccggg gcagtgctct ttttttataa atgtttatgt
    13081 ggatgaccat aattttaatc tgttttataa ttgacgaaaa ttggaggttt agaaaaccaa
    13141 ttttctcgta gtgggtatgg agaatgaata tcctatcttc aaaaccactc caactgtgga
    13201 aactaaaaag cgggaggaag gaaatgctgg ggaataaaag tatcaacaag gataatcact
    13261 tagaaattgc cggatgtgat gtagtaaatt tagctcaaac tatggggact cctctttatg
    13321 tttatgatga ggctctactc cgttctaata tgcaggaata tgagaaaagt ttcagggaaa
    13381 actatcccca tttttccctg gcttatgcag gtaaagcttt tttgtgttcg gctttgtgct
    13441 ccataataaa agaggaagat tggtggttgg atgtagtgtc aggaggagag tattatctgg
    13501 cctctaccgc taattttccg gtagagaaaa ttatctttca tggtaataat aaaactaatg
    13561 aggaacgccg cctggttctc cgggagggag tagggcggtg ggtggtagat aacgaagtag
    13621 agctaaatct tctcctggag gaagcttcat cgtatgcttc ttacaaagtc cctctttttt
    13681 ttcgtcttac ccccggagtc gacccccaca ctcatcagta tatcaccacc ggtaaaatcg
    13741 atagtaagtt cggttttctc ttaggagagg cagaaaatgc tatattaaaa gcaattcagt
    13801 cggaagattt agaagtgtgg ggtctacact gtcatattgg ctcacaaatt gaccatatag
    13861 agcctttttt gaaggcggca gaagttatgc tccaatttat ggttgatttt aggaaaaagc
    13921 acggttttgt gtttcgagaa ttagacctgg gaggaggatt aggagttcct tatacagatg
    13981 aggaaaaaag ccgttttccc tcttctcatt tttatgtaaa ggaaataagt gaaaaagtaa
    14041 aggaaatgtg cggggagctt tcttatcctt tacctcgtct ttatatagag ccgggtcgat
    14101 cggtcgttaa tactgccggt agcaccattt accgcgtggg gagcgtgaaa gagattccgg
    14161 gaattaaaaa atatgtggct gtagacggag gaatgaccga taaccctcgt cctgttttat
    14221 acggtgctaa atatcaagct cacattggta atcgagtagc aggagaaaga gagaaagttt
    14281 ctgtggtggg aaaatgttgt gaatcgggag atgttttaat agaagaaatt gaacttcctt
    14341 ctccttctcc cggggatgtt ttagtagtgg aagccactgg agcttataat tattctatgg
    14401 cttccaatta taatcttatt ccccgccccg gggtggtttt tatccgggag ggtgagccgg
    14461 aattggtggt gcggccagaa aactggtatg atttagtgag aagagacatt actcctctgg
    14521 agtgataaag aaggtgagag ataatgaaag agattggagt atttttagta ggagcagcgg
    14581 gcaaaatggg gaaagaaata gtaagagcta tccataaaga agaaaatata gttttacgag
    14641 gagctgctga taaacaagaa gtgggcagag atacaggaga ggtatcgggc gtgggacctc
    14701 agggaattat aatagaggaa aatttagctg aagcgataaa tagagtaaag ccagatgtta
    14761 tttgtgattt tacccacgct tcagctctgc gggaaaacct tcctctattt ctggattata
    14821 aaattcctct ggtgattgga acgacgggtt taagtagtga agaaatagaa actctgcgcc
    14881 agagttgtga gagagaaggg ataggttgtg taattgctcc taattttgct ctgggtgcgg
    14941 tattgatgat gcatttttct cgcatcgctg ctcgatattt ggagcgagca gaaataattg
    15001 aatatcatca ccctcagaaa aaagatgctc cttcaggaac agcgataaag actgctcagg
    15061 ggatagagga agttaaaaat actccttttt ctgataaaag cgaagagata ttatccgggg
    15121 cacgaggagc acttctgggc tctgtgaata tccacagtgt gagattgcct gggcttgtag
    15181 cccatcagga agttatctgg ggaggagagg gagagattct caccataaga catgattctc
    15241 ttagccggtc atcttttatg cctggagtga tgatgtccat taagaaagtt tatgaggaac
    15301 cccgatttta ttacggattg gaagaagtta taggtcttac ctcttgatag atgtgataaa
    15361 atttacttca aattgaggag aggaaaatgc agtttgtaaa aatgcatggt ttaggtaatg
    15421 attttattat tttccctgaa gaggagaccg gaggggtctc ttcctggcct tctttttctc
    15481 taaaaatttg tgaccgtcat aaaggggtgg gaggagacgg agtgatttta gttctatctt
    15541 cctcagtggc tgacctccgg atgcgtattt ttaactctga tggtagtgaa gcagagatgt
    15601 gtggtaatgg aattcgctgt ctggctaagt ttgcctttga gcgggggata gtaaacaaaa
    15661 aggagttcac agtggaaact aaggcgggca tcatcatccc ccggattaca gaagaagaag
    15721 aggggaaggt aaggagggta agagtgaata tgggaaaacc ccgcttccaa ccccgggaaa
    15781 tacctgtgga tttagaggga aaagaggtaa agaattaccc tttagaggta gaaggtagaa
    15841 ctttccttat ctctgctctc tctctgggta accctcatgt ggtgattttt gaaattcctc
    15901 gcgattggga aaaatacggg agagaaatcg aaaaccatcc tctgtttcct gaaaaaacca
    15961 atgtagagtt tgtagaggta agaaacaaga aagaggcggt ggttaaagtc tgggaaagag
    16021 gagcaggggt cactctggct tgcggaactg gcgcggcggc ggtattagta gcttcggtaa
    16081 gagaggggaa attagacaga aaagctgcta tacacttgcc cggcggaaca ttagatgtgg
    16141 agtggcagga ggaggtctac attgaaggac cggcagaaga agtgttctcg ggagaaatta
    16201 atgaggaggt agagaaagaa tggaagttgc tcagcgtata aagaaccttc ctccctacct
    16261 ttttgctgaa attgagaaaa aagtaaatca agctcgagcg gaaggtaaag aaattattga
    16321 tttggggatt ggagaccctg atattcccac tccctctttc attgtagagc ggttatgtga
    16381 agaggcgtgg cgtcctgaaa atcatcgtta cccttcttat cagggattag atgcttttcg
    16441 agaagcggtg gttttatggt ataaaaaaag atttggtgtt gaactggatc ccgagcaaga
    16501 agtagtttct ctcattggtt ctaaagaagg gattgctcat cttccctggt gtctggtaaa
    16561 ccctggagat ttagttttgg ccagtgaccc gggttatccg gtatataaga ttggtacaat
    16621 gcttgccgga gggatacccc ttgagcttcc tcttctggct gaaaatgatt ttcttcccga
    16681 tttatatagt tttcccgata gtatatggga aaaggttaaa atagtttttt tgaattatcc
    16741 taataatcct acagcaagca ttgctcctct ttcttttttt gaggagatag tagagttagc
    16801 ccgacgttat gattttgtaa tagcccacga tttagcttat tcggagataa gctacgatgg
    16861 ctatcgagct ccttccattc tggaggtaga aggagctcgg gaagtagcgg tggaatttca
    16921 ttctctttct aagaccttta atatgacggg ctggagaata ggttttgcag taggcaatga
    16981 aaaagtgata gaggctctgg ggagagtgaa aactaatatc gattcaggga tttttaatgc
    17041 cattcaatgg gcaggggtgg aagctctaaa tagggtggat gaggtattaa aaaatattat
    17101 tcccgtctac accgagcgcc gtaacctggt ggtggaaact ttttcctcat tgggttttga
    17161 catcacccca cctcaggcca gtttttattt gtgggttcca gttcctccag ggtttacctc
    17221 cctggaattt accaactatc ttctggataa atctcaagta gtggttactc ccgggactgg
    17281 ttttggtagg tatggagaag gatttgttcg aatttctatt actactccct ctcctgtttt
    17341 agaaaaagct atgatgagga taaaagaaac ttgggatagg tgaatgaccg tgaaaatagt
    17401 ggtacaaaaa tttgggggaa cttcagttaa tacccgggaa cttagagaaa aagcagcttt
    17461 gaaagtgcag gaaactcaaa aagaaggatt ttccccggtg gtggtggtat cggctatggg
    17521 aaggaaagga gcaccttatg ctaccgatac tttgatttct ctggctcgag aggaattttc
    17581 taatcttgac ccccgggatc tagaccttct tctctcttgc ggagagatca tctcagcagt
    17641 agtgatgtct caaaccttgc gccggatggg aatgaaaagt gtagccttct ctggagctca
    17701 agcaggtatc attaccgata atcattttgg cgatgcttct atactgaggg tagagccgga
    17761 tagaatcaaa aaatatctgg ataaaggata tgtagtagtg gtggctggtt ttcaaggggc
    17821 aacggaagaa ggggaagtca ccactttagg aaggggggga agcgacacta cagcggtagt
    17881 tctggggatt tatttacaag ctgattatgt agatattttt actgatgtga acggagtttt
    17941 taccgctgat ccccgcatcg ttcctgaggc tcaaattctt caggggctaa cctacactga
    18001 agctgcagag atgattcagc agggagcaaa agttattcat cacaaagcta tgaatctggc
    18061 ccgggagtac cggatacctc tccgagtcag gagcttaagc gatgaaggca aaggaacttt
    18121 aatctgtgat atttccactc tcactaaaga aggattgaga atcctttctc aggaagagcc
    18181 ggtttatagc atcgcttacc gccggggttt agctcaagtg cgagtgaatt tgggagattc
    18241 ggaaaacgga gagctaaaaa tttttcaatc tctggcccag gagggcatca gcattgacct
    18301 ggttagcctt tttcccgaag gcacggcgtt tgtagtagat gagacggtag ggagtaaggt
    18361 agaggaaatt ttgcgtcggg aagggtttga ggtaaacctg actctccctt gtgctaaagt
    18421 ttctctggta ggttctgata tggccgatcg tccgggagta gtagctcaat tagtagaagc
    18481 ccttaaagat gccaatatag agattctcca gactggagat tctcatatta ccatttcttg
    18541 tctggttaaa tcccaggata tggagagggc cattcaagtt ttacaccaca aatttcgact
    18601 ggaaaaggcg tgttaggggg gagcaaaaag caatgggata ttttggcagt cttttaactg
    18661 cagcggttac tccctttgac caggatttaa cggtaaatca ttttgaattt cggaaattat
    18721 tgcgggcttt ggtggaaaat ggttcggatg gagtggtggt ttccgggact accggggaat
    18781 ctccgacttt atcccgggaa gagaaactct gtctctttga agtggctctc gaggaagtgg
    18841 gagatcaggc aaaagttata gcaggaacgt gcaattacaa tactaaggaa tccgtacttc
    18901 tttctaaaga agcggaaagt ctgggagtac atggtattct ggcagtggct ccttattata
    18961 acaagccccc tcaagaaggg ctttaccagc attttcgtgc catcgctgag gcagtaaata
    19021 ttccagttat gctttataac atcccttctc gcaccggagt gaatattgag ccagaaacca
    19081 tagctcgcct atcagaaatt ccgaacattg tagccctaaa agaagcagga ggaagcaccg
    19141 atcagatttc tcgaattaaa gctctggttc ctcctgattt ttatctttac agtggagatg
    19201 atatcatgac tcttcccctt ttagctttgg ggggagtggg agtggtaagc gtggcttctc
    19261 atctggtggg caaagagata aaaaagatga ttcagctatt cgatgaagga aaaactaaag
    19321 aagctctgaa agtccatctt gagctttatc cactctttaa agctctcttt atctccacta
    19381 atcctattcc agtaaaagct gctctaaacc tggcagggtg gagagtggga ggggttcgac
    19441 ctcccctgtc ttccttagaa caagaaaaag aggaaaaatt aaagtcagtt ctggaaaaat
    19501 tccacttgat ttaattacca gaaaatgttt aaatatctat caaagcgatg atagggaaga
    19561 gattagcgtt atggttcagc gaggaaggta gggtgcgagc ttccaccgtg gcgttaagga
    19621 agatagagcc ctggagctga ctccgaagaa aagagttttt ctctaagtag gagctccggt
    19681 tgagccgtta aaacaaagag tatagcaggg tggcaccgtg caccaagtgt tgcacccctg
    19741 cccgtctttg aaaacctcgt ctaaagacga ggttttttta ttggtaagaa ggaggaataa
    19801 tgatggtacg tgtaggagta atgggagcta ctggatacac tggtcttgag cttttacgca
    19861 ttttggctta ccaccccggt gtagaagttt gctgggtagg aagtgaaagt tttccgggta
    19921 taaaattgga ggattattgt ctggcctggc aaggaagaag caatttgact ttacaaaagc
    19981 aggaagtgga tagtttagaa ggaatagagg caatttttct ggctcttccc tcaggagtag
    20041 ctatggatta tgttcctcct cttttgcaaa aagggatacg ggttattgac ctgggggctg
    20101 attttcgctt taaaaacgta gaggttttcc aaaaatatta tcagttagaa catcgtgttc
    20161 ctcacctttt acaggaagca gtatatggta ttccggaaat ttgtagagag cgcttaaaag
    20221 aggcgcaact ggtggctaat cccggttgtt atcctacttc ggtgattatt cctctgtatc
    20281 cctttttaaa agagggagta atttcacctt ctataatagt ggatagcaaa tcaggagtaa
    20341 ccggagcagg tagaaagcct actgaagaca accagttttg tgaggtagat gaaaatttca
    20401 aagcctataa agtgggagag catcgtcatc agccggagat gaaagaacag ctggagttag
    20461 ctaccgggag ggaggtcaac ctcattttta ctccccatct tttgcccctg aagagaggga
    20521 ttttaactac tgtttatctt accctggaaa aagatataga ggaggaggag cttttatcta
    20581 tatggaagga ttattgggga aggaaaccct gggtgcggat tttccctctc catcgttttc
    20641 ctgagctgcg ctgggtagta ggctctaact ttgttgatct gggactcaaa aaggtgggag
    20701 agaatcagct gatagtaatt tctgctctgg ataac
//
LOCUS       DAOPSS010000012        20722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2168_length_20722_cov_9.399139, whole genome shotgun sequence.
ACCESSION   DAOPSS010000012 DAOPSS010000000
VERSION     DAOPSS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 20722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20722
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_20722_cov_9.399139"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="PKN31_01545"
     CDS             complement(<1..1326)
                     /locus_tag="PKN31_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOA98646.1"
                     /translation="MAGITGIFAPGKKKEVEKMLNKISHRGLKGKAILELKEATLGVV
                     WPALYEPSLARWKKEKMVKDDRGTDHFALARVREKEPELKRDYIGVAPLYYIFNERGD
                     LCFSSEVKGLLSYGRKIKEMPPGSFFYKEKVNPYFRWKKLPLFRGELKIVAQKLRDKL
                     SKAVKKRIKSNPAGSLLSGGLDSSVVASLARPRVQKMYTFSVGVEDAPDLYYAREMAK
                     FIGSEHREIVVDLEGMLEVLPKVIYHLESFDAPLVRSSIINYMVANLASDYVPEVFSG
                     EGGDELFAGYDYLKSLNKDRLPQELRDITFSLHNTALQRVDRCASAHGIVAQVPFLDQ
                     EIVDYAFQIPVDLKIKNGVEKWILRKAVEDLLPEEILWREKSKFWQGAGVEELLYQYA
                     EENITDEDFGKERVLPNGWVLRSKEELMYYRIFRDFFGELQDLSWMGRTK"
     gene            complement(1342..2604)
                     /locus_tag="PKN31_01550"
     CDS             complement(1342..2604)
                     /locus_tag="PKN31_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA98647.1"
                     /translation="MTSRERVISSLSHREPDKVPLDLGSTLITGIHVSSLHKLKVALG
                     LIKPEEPVKVIDPFQMLGEVDDELRKVLGIDTVPLLSLYNFFGFKNENWKPWTFFDGT
                     PLLVPEKFNTTPDSEGNIYQYPRGDSTLSPSAKMPRGGFYHDAIIRQKPFREEELKVE
                     DQIEEYSLLSEEELRYYESEAQKLYEETEYSIVSSGVPGINLGDIAYVPGSALPDPRG
                     IRDVEEWYVSLLTRQDFIREVFSHITDIGLQNLELFHQAVGEKIQVIVVSGTDFGSQN
                     GLFISQEVYRKLFKPFHQKINNWIHHNTTWKTFIHTCGSVYDLLPDIKEAGFDILNPV
                     QISAAKMDPENLKKDFGHQFTFWGGGVDTQRTLPFGAPDEVKEEVERLIKTFKPGGGF
                     VFATVHNIQANIPEENLLALFEAINEYR"
     gene            2807..3340
                     /locus_tag="PKN31_01555"
     CDS             2807..3340
                     /locus_tag="PKN31_01555"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HOA98648.1"
                     /translation="MKRFYFLLVLSILVTSFSPLSFAQESLTTYISSAGFSLTFPPDW
                     EVYNPPSTPNPYLVMLGQDSEAQVGVTLEYYNYSSLEDFFKKVKEEVNQFPGIEIISQ
                     GGESINGAPSCWFTYTFLVSSEPSASQEPFQAIIYLFSQSNNFFRIICLTKESQFNDY
                     LNTFQAIARSFTTSVGN"
     gene            3395..4033
                     /locus_tag="PKN31_01560"
     CDS             3395..4033
                     /locus_tag="PKN31_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOA98649.1"
                     /translation="MEKEGAKRILCFGDSLTWGWDPETQERYSAGSRWTTILQKNLGE
                     SYWIIEEGLNGRTTIWDDPIEGNKNGRRHLPILLESHRPLDLLIIMLGTNDLKARFQV
                     TALQIAQSAGTLVEIAKRSDAGRGGNSPSVLLISPPPLGKLAIWQEEFGEGTEKSQNL
                     ARYYSYFAQVNQCHFFDAGEVVKTSDIDGIHWEVAANRQFAEALTNKVKDIL"
     gene            complement(4030..4269)
                     /locus_tag="PKN31_01565"
     CDS             complement(4030..4269)
                     /locus_tag="PKN31_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98650.1"
                     /translation="MAIKREKYRDKGIQEVYNTPYEKKLKKLPWIQDKRVRERYLRPE
                     EENKILDDLVCLKCGEKCGGNCEDRESLRATNIFS"
     gene            complement(4276..4998)
                     /locus_tag="PKN31_01570"
     CDS             complement(4276..4998)
                     /locus_tag="PKN31_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA98651.1"
                     /translation="MENDLMLRIEDLHVFYGGIHALKGINIEVPAGKIVALVGANGAG
                     KSTTLRTVSGLVRARQGKIEFQGIDITRESAHHIVEMGLAMVPEGRRIFPNLTVWENL
                     LLGSYTRRDKEEIKEDLDWIFRIFPRLKERVRQKGGTLSGGEQQMLAVARALMSRPHL
                     LMMDEPSLGLAPLLVKEIFNIIREINQNGTTILLIEQNAKAALQIAHYAYVLETGSVV
                     LEGRGKELLENEKVRKAYLGEE"
     gene            complement(4998..5780)
                     /locus_tag="PKN31_01575"
     CDS             complement(4998..5780)
                     /locus_tag="PKN31_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA98652.1"
                     /translation="MKVENKEPLLSLENLTVAFGGLVAVSDFSLQLFSGDLVGLIGPN
                     GAGKTTVFNLITGQYRPTKGRVIFQGKDITGFSPDLVTTVGIARTFQNIRLFGNLTVL
                     ENVLVSFHVRLQSSFLAPMFSLPSYWREEKAIRREAYDLLEAVGLADVTKEEAQSLPY
                     GEQRRLEIARALATHPSLLLLDEPAAGMNPQETQELMEFIKKMRDDFNLTIFLIEHDM
                     KVVMGICERIAVMDFGMKIAEGTPEEIRNNPRVIEAYLGVED"
     gene            complement(5764..6825)
                     /locus_tag="PKN31_01580"
     CDS             complement(5764..6825)
                     /locus_tag="PKN31_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOA98653.1"
                     /translation="MGREEKRDLALTLILFAVLMLGVSLAERYLNTYLIRILNTACIY
                     VVAAVAYNLINGITGQFSLGPNGFMAIGGYTVAILMLPLVQKEQVYFLQPLIWPFNSF
                     SFPSHLFLLALFLGGVAAALAALLIGIPTLRLRGDYLAIATFGFGEIINVLANNFIPL
                     TNGALGIKGIPEYTNLWWTVNWAFWSVFMIKGLVNSSFGRALKAIRENEVAAEAMGVN
                     LFRHKLLSFVLSGFFAGVAGGLFVTLISTVSPSLFTFTMTFNLLIIIVLGGLGSITGS
                     VVTAFIFAFLQEILREVEAPITIGAWTFPGIPGMRMVVFSVLLVVMMIFYRRGLFGDK
                     EISWQLIIDRIKGRNESGK"
     gene            complement(6830..7723)
                     /locus_tag="PKN31_01585"
     CDS             complement(6830..7723)
                     /locus_tag="PKN31_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOA98654.1"
                     /translation="MTWVSFFQQIMNGVSLGSLYALLAIGYTLVYGILGFINFAHGDV
                     FMLGAYFSFFMIGVFLLPWWLGVIIGVALCAIVGMSIERIAYRPLRDAPRISALITAV
                     GVSFFIENLGLVTVGARPKGFPRPELMKNVYILAGARIQGVTFWVPIISVSVLLVLLY
                     LVYRTKMGMAMRAVSKDMETTRLMGVDIDRIILYTFALGSALAGIGGVLWACKYPQIN
                     PLMGTFPGWKAFTAAVVGGIGNVVGAMVGGFIIGLTEIMIVAFFPGLSGYRDAFVFTI
                     LIIFLLVKPSGILGETLKEKV"
     gene            complement(7778..8953)
                     /locus_tag="PKN31_01590"
     CDS             complement(7778..8953)
                     /locus_tag="PKN31_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA98655.1"
                     /translation="MVILWQVFTKILERGILELRRLISLFTVFLLVLSFSGIVLAQET
                     VKIGANLEITGGVAAYGQMIWEGVNIAKEVVGDEVLGKKVELVLVDNKSDRVESANAA
                     TRLIDYEKVVVMVGPAISGSMLAVGPICEEKQIPVISASATSPLVTQGKKFVFRACFR
                     DDDQAKAAAQFARSELQAQTAAILSDIAQDYCVALGNFFKDAFIEKGGQVVSEQNCRS
                     GDQDFSAQLTTIINANPDVIYVPNYYTETALICQQARDLGYEGMILGADGADAPELVS
                     IGGDSVEGVYFTAHADTTQAITEIGQKFFDLYREKHGREPSAFEALGGDSYVIILDAI
                     KRAGELDPVKIRDAMEDTENLEVITGVATIVDGDNKKPIVVKRVENGVFKFVTTIEY"
     assembly_gap    9242..9341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9469..10599
                     /locus_tag="PKN31_01595"
     CDS             9469..10599
                     /locus_tag="PKN31_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA98656.1"
                     /translation="MLFLSHFLADFFASFPSPLSPYFIGVYGVDSRTIAMVIATLSLL
                     SSLSQIFFATRPLFQKSEVQFYISLSLLILPWSLIPFSSHIYTLFLILVVAFLANASF
                     HPQGAALSGESGSQGITLFVSGGIAGAALGPIFITWIVSSGGISGIVPLTLGIWICWT
                     ILFLIILRKRDYNISSSIPRQKFSLQFFLPLLPIWIMVSIRTFFTDIFHTFVPIYLNF
                     QGYSLVMGGMLLSGGVMSGLLANSIGSRIRTQFNNRLVNLLSFGGLGVAILIFTRAEG
                     IISLTLSYLLADFSSFLSMSTNVCEAQDLFPQHRAFASSVAMGLSWAFGHFLHLLYSS
                     LLGNRVTLVIESAGFFSLGMTVFLLLFPSFFQRKAYCGVESS"
     gene            10596..11786
                     /locus_tag="PKN31_01600"
     CDS             10596..11786
                     /locus_tag="PKN31_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA98657.1"
                     /translation="MRILHNWSKKRGSTNFFIFLSIAFASNFIFALTSVLFSIFATDM
                     GFSPPEVGLAISAFTAGSLIGAPLWGKVVDKFSKRKVTLLISLLGQALFCLLIPLMQC
                     LLGLVIVRFFFGFFMVAQALTLNELTVKIEEEERRSKEISAINVARAAGYSLGCLLSG
                     FLGDLHPNSSFYLGSSVVILIFFLTFYLQEETGKAVPFSQEDNPAKHWILQKRVALFY
                     FSIFLRSTAVNGLSYFLPLFWKNMGQTTALIGSVIAISNLAQLFFFPLSGKLSSRSDQ
                     MAFRSTRFGLLLTVIPFLIAPFFHQGVLILLPQCILSISWAFFYIGTILSLRSFVPLQ
                     KQGEALGWLEVALNLGGIVGPNLFAFSLSSFNNNFPSAFFSLSFLPLIAFFISRKNEK
                     NFLS"
     gene            complement(11794..12045)
                     /locus_tag="PKN31_01605"
     CDS             complement(11794..12045)
                     /locus_tag="PKN31_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOA98658.1"
                     /translation="MDNRVGVVGIIVTDREKQAERVNEILGEFGELIVGRMGIPYRER
                     NVSVIALIVDGSTDELGALTGKLGSIPGVKVKSALVSSS"
     gene            complement(12038..13513)
                     /locus_tag="PKN31_01610"
     CDS             complement(12038..13513)
                     /locus_tag="PKN31_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOA98659.1"
                     /translation="MALLLGIDVGTTNTKLRVYNHDGEIKEERSFPTPLFPDEEGELY
                     HPQKILHQILQAISQFSGELRREIIALSVSSYAEVLVKVGENGEPVGGSIPWYDNRTE
                     KYFREIELDKEEVYRISGLYPYHIYSFYKLFWHREREKEAFEKTKFWTSMSGFILFYF
                     SGIPSFDYSLASRTMLFRQKERCWWEEMLARAGLSAKDMAPLYPSGVVLGTVREEVAR
                     DCGLSPDTLVVTGGHDHLCAALSVGVYREGRALISTGTTESLTMALENLPSLSPSGEE
                     KPFSWGHHVAYPYYYAMNGIYSGGYAVDWILRLLKEDYAFLENLSPHFSPRVPIFFPY
                     LLGGDYEGAKGAFLNLTGGAEREELILGLLLGLCFEYRNIWEKMEKNLGFRIKETANV
                     GGGTQNRVWMELKATTLDKNIPVPRDREGSCRGAALLAGLGAGVYKDFEEAFQKTFRV
                     REVFEPQKEWQESLEKWFRVYVEVEKDLREINEKLGVLYDR"
     gene            complement(13515..14360)
                     /locus_tag="PKN31_01615"
     CDS             complement(13515..14360)
                     /locus_tag="PKN31_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOA98660.1"
                     /translation="MDILMPTVLIGVLSLIFGVMLAWFSQKFAVEVNPLVQKAEEILP
                     GANCGACGFPGCSNLAEKIVAGEAPPDACPAGGPKVWAELSALVGKEVDSHREAQKAV
                     LLCQGAREVAGEVAVYQGLKDCKAAHLLGNSFKSCRYGCLGFGNCVRVCPFEAMIMDE
                     EKGLPIIIEDKCTGCGKCVEECPRSIITLLPAREQVVEACVSLASPRETRQSCKKGCI
                     KCSICVKSCPEGAITLEGGRIKIDRDKCNLCGVCIEKCPTKCLIWVGNELVLEEKELE
                     AKVGG"
     gene            complement(14370..14945)
                     /locus_tag="PKN31_01620"
     CDS             complement(14370..14945)
                     /locus_tag="PKN31_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOA98661.1"
                     /translation="MDLFKIFISAALVDNIILARYIGLCPYIGMSSNVANSLGMGMAV
                     TFVMVLASIATWALWNFIFVPLGLEYLQIMVFILVIAVLVQLLEAILKKTVPNLYQAM
                     GIYLPLITTNCAILAVTFFGVDYNYNLLQVVVYSIGVALGFTIALLLLAGIREKLRFS
                     KVPQFYQGYPIVFISTCLLAIAFMGFKGLVK"
     gene            complement(14951..15550)
                     /locus_tag="PKN31_01625"
     CDS             complement(14951..15550)
                     /locus_tag="PKN31_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOA98662.1"
                     /translation="MKEFWDCFKNGIVGENPVLRLMIGLCSVLAVSVRLENCLFMGAA
                     VIFVLTGSNLVISAIRNFVPNEVRIPIFIVVISTFTTIVDLVMKAYLPALSEALGVFI
                     PLIVVNCIIMARAEAFASRKPVILTIADSLGMGVGYTLVIAAIGVVRELFGYGQFFYH
                     QIFPSSYQGMMIMILPPGAFLLIGIYIALLNHFHRRDNL"
     gene            complement(15547..>15963)
                     /locus_tag="PKN31_01630"
     CDS             complement(15547..>15963)
                     /locus_tag="PKN31_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOA98663.1"
                     /translation="IAPFAQEFKKWETLPEAQDSIKDLSILEVYEALQEGKREGLVFR
                     VSTSGYGGPIILLVGISEEGKLTGMEVLEHQETPGLGSNIESADFLNQFIGKSLIDPF
                     EINRDIEAISGATISTRAIVRACQGLASYWEEVREQ"
     assembly_gap    15964..16063
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16064..16959)
                     /locus_tag="PKN31_01635"
     CDS             complement(<16064..16959)
                     /locus_tag="PKN31_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOA98664.1"
                     /translation="MNEELKLVITSSPHLRDETTVPWIMRQVVLALLPATIWGIYFFG
                     VSSTLLTILLSVGSTIGFEALNCKIFKKPLSITDGSAVITGLLLGLSLPPSAPFWLPL
                     VGGGAAIFLGKEMFGGLGQNIFNPALVGRAVLLVSWPKAMSTWWVSAPFGFSGFSGGG
                     AASTFSGVEMVTTPTPLSIAQMYGYSSLHQADPRILWRLFVGQVPGSIGEISALLLLI
                     GFLYLWWRGVVHWDIPLSYFVGLSCVAILGGQSALFHILSGGAIMGACFMATDYVTSP
                     ISSKGRYIFGLGAGAITGVIRL"
     gene            complement(16956..18281)
                     /gene="rsxC"
                     /locus_tag="PKN31_01640"
     CDS             complement(16956..18281)
                     /gene="rsxC"
                     /locus_tag="PKN31_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOA98665.1"
                     /translation="MRLNTFPGGIHSPTFKEFTATKPIKEFPFPSRVVLPLLQHTGAP
                     LSPLVKKGDRVKRGQKIADVEAFISAPLHSPLAGKIEGVEDYFHPLLSKFPAITIEND
                     GSGEEERKDPLCDIENKDPAFLREWIREGGMVGLGGAAFPTHVKVSPPPGKKIDTFIL
                     NGAECEPYLTCDYRVMIERSSAILTGLRVLVRACGAERVFIGIEENKIEAIEKFDKLV
                     KDLPFPARVVPLLTKYPQGSEKQLIVAILGREVPPGGLPLDVGVVVNNVQTAWAVGRM
                     AEEGLPLIERVITLSGECLKSPENLQVPLGTVLSDLVDFCGGWVETPKKVIMGGPMMG
                     VAQTSLQVPVVKGTSGFVFLSNLYEKKEYPCIRCRRCIDACPMRLFPTMLGKFSEYQA
                     FDEARDQRVLDCMECGACSYVCPSNISLTHLIRLAKAEVMRRSRKGGNQ"
     gene            complement(18305..19651)
                     /locus_tag="PKN31_01645"
     CDS             complement(18305..19651)
                     /locus_tag="PKN31_01645"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOA98666.1"
                     /translation="MFGKILIANRGEIALRVVRACRELGIKTVGVFSDPDYNLLHLKF
                     VDQKVALGGSLPQDTYLNEEKIIRACELTEVDAIHPGYGFLSENGAFARKLEEKGITF
                     IGPDADLLERIKDKLQAKRILEEAGIPVVPGSREPLKDENEARKIAEEIGYPLFLKAS
                     RGGGGRGIRVVREPGQLAGALESAKREGKLAFGSEEVYMEKLLTKARHVEVQIVRDKE
                     GRVAHLGERECSLQRRQQKILEEAPAPFLTEETREKLREAAIKVAEVLNYTTCGTVEF
                     LVTEDGSFYFMELNARIQVEHPITEMVSGIDLVREQICLAAGEKLSFSPEEVSLRGHA
                     LEMRINAEDVAHNFSPSPGKISRFDVPRGPGIRVDSHCYSGYEVPPYYDSLLAKLIVW
                     DCDRDFAIRRAREALHSFAVEGVSTTIPFHMKILQNRDFMTGNLHTKLIEENLEEY"
     gene            complement(19664..>20722)
                     /locus_tag="PKN31_01650"
     CDS             complement(19664..>20722)
                     /locus_tag="PKN31_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOA98667.1"
                     /translation="YMTLNTILALALRRRIEYRTRLAIRDSFSLDFPGGVVRFVLSVL
                     KYTFLIEGIGFMILFLNFLRYFTPGEAIFPALFHSISAFCNAGFSIFSSNLEGFVLDP
                     VVNFTIMGLIILGGLGFVVLREIVEKRGLSSFHSKVVVRTTLFLISLGTILILLLEWG
                     NPSTLAELPPGGKIMAGLFQAVTPRTAGFNTVPTAGLREASAFLIMLLMFIGASPGGT
                     GGGVKTTTFALLLGVVMNIVRGGDKVEFFHRRVAPATVFRAISVVVLAFFLVFGVIMV
                     LLVIQPAPFLDICFEVFSAFGTVGLSRGITAGLLDLSKFLLIITMYVGRVGIMTLVML
                     RPSREKDLITYPEDRILI"
BASE COUNT         6161 a         4958 c         3524 g         5879 t
ORIGIN      
        1 tttcgttctt cccatccagg acaaatcctg tagttcccca aaaaaatccc gaaagatacg
       61 ataatacatc aattcttctt tgctcctgag cacccagcca ttgggaagaa ctcgctcttt
      121 gccaaaatct tcgtcagtaa tattctcctc agcatactga taaagtaatt cctctacccc
      181 ggcaccctgc cagaatttgg atttctctct ccataatatt tcttccggta ataaatcttc
      241 cactgctttt ctcagaatcc atttttctac cccgtttttt atctttaagt caactggaat
      301 ctgaaaggca taatctacta tttcttgatc taaaaaggga acttgagcca ctatcccatg
      361 agcagaggca caacgatcta ctctctgcaa cgccgtatta tgaagactaa aagttatatc
      421 tctcaactcc tgaggtaatc tgtccttgtt caaagatttt agataatcat agccagcgaa
      481 aagctcatct ccgccctcac cagaaaaaac ctccggaaca taatcagaag ccaaattggc
      541 taccatataa ttaataatac tggaacgaac cagaggagca tcaaaagatt ccagatgata
      601 aataacctta ggtaatactt ctaacattcc ttctaaatct actactatct ctctatgttc
      661 tgagccaata aatttagcca tttccctggc ataataaagg tccggagcat cctctactcc
      721 cacggaaaaa gtatacattt tttgcactct cggtcgagct aaacttgcta caacgctaga
      781 atcgagaccc ccggaaagca aagaacctgc tgggttactt ttgattctct tttttactgc
      841 tttactaagt ttgtcacgaa gtttttgagc tacaattttc agttctcccc gaaaaagagg
      901 cagctttttc cagcgaaaat aaggattgac tttttcttta taaaaaaagc ttcccggagg
      961 catttctttt atttttcttc cataagacaa aagtcctttt acttccgaag aaaaacaaag
     1021 atctcctctt tcattaaata tataataaag aggagccact cctatataat ctcgctttaa
     1081 ctccggctct ttttctctta cccgtgccag cgcgaaatga tcggtccctc tatcatcttt
     1141 taccatcttt tcttttttcc atcgggcaag cgatggctca tagagagcgg gccacaccac
     1201 tcctaaagtc gcctctttta attctaaaat tgctttacct ttcagacctc gatgagagat
     1261 cttatttagc attttttcta cttccttttt ctttcctgga gcaaaaattc cagtaatacc
     1321 agccaaaata attttacctc ctcatctata ttcatttatt gcttcaaata gggctaataa
     1381 gttttcctca ggtatattag cctgaatatt atggactgta gcaaacacaa atcctccacc
     1441 aggtttaaag gtttttatca accgctctac ttcctctttt acctcatctg gagctccaaa
     1501 aggaagagtt ctctgcgtgt ctactcctcc accccagaaa gtaaattgat gtccaaagtc
     1561 tttcttcaga ttttcaggat ccatcttagc tgcggatatc tgcaccgggt ttaagatatc
     1621 aaaacccgct tcttttatat caggtagtag gtcataaact gagccgcagg tgtggataaa
     1681 agttttccag gtggtgttgt gatgaatcca gttattaatt ttttgatgaa agggcttaaa
     1741 taactttcta tacacctcct gggaaataaa aaggccattt tgcgaaccaa aatcagttcc
     1801 tgaaaccact attacctgga ttttctctcc cactgcctga tgaaataact ctaaattttg
     1861 caagccgata tcagtaatgt gagaaaaaac ttcccggatg aagtcctgac gagtcagaag
     1921 agatacgtac cattcttcca catctctaat tccccgagga tccggcaaag ctgaaccagg
     1981 aacgtaagca atatctccca gattaattcc cggaacccca cttgacacta tactatactc
     2041 ggtttcctca tagagcttct gagcttcact ctcataatag cggagttctt cttcagaaag
     2101 taaactgtat tcttctattt gatcttctac tttcaactct tcctctcgga agggcttttg
     2161 cctgataata gcatcgtggt aaaaaccgcc ccggggcatt ttagctgagg gagaaagagt
     2221 actatccccc cgggggtatt gataaatgtt accttctgag tccggagtag tgttaaactt
     2281 ttccggcact aaaagggggg tgccatcaaa gaaagtccaa ggcttccagt tttcattctt
     2341 aaatccaaaa aaattataga gagagagaag aggaacggta tctattccca gaacctttcg
     2401 caactcgtca tccacttctc ccagcatctg gaaaggatca attactttca ccggctcttc
     2461 cggcttgatt aaccccagag ctacctttag tttgtgcaag ctggaaacat gaattccggt
     2521 aatcaaggta gaacctaaat ccaggggaac cttatctggt tcccgatggg aaagagaact
     2581 tatcactcgt tctcgagacg tcatattctt tcctcctttt atattcttta tttctttaat
     2641 tataactaat attttcaaat agattataca agaataacgt acagcttaac attaaaaata
     2701 aaaagatgag aaaaaactaa aataaattat gctggaattt aactatgaag aacaaaacat
     2761 atttataaaa tgatataatt taaaaaaagc cttctaggag gaaaatgtga agaggttcta
     2821 ttttctcttg gttttgagca tattagttac atctttctct cctctatcct ttgctcaaga
     2881 aagtttgaca acttacattt cttcggcagg attctccctc acttttcccc ctgattggga
     2941 ggtctataat cctccttcta ctcccaatcc ctatcttgtg atgttaggcc aagactcaga
     3001 agcccaggtc ggggtcactt tagaatatta taattactct tcattggaag atttctttaa
     3061 aaaagtaaag gaggaagtaa atcaattccc gggaatagaa ataatctctc agggaggaga
     3121 atccatcaac ggagctccta gctgctggtt tacctatact ttcttggttt cctcagagcc
     3181 ttctgcttct caagaaccct ttcaggctat tatttatctt ttttcccaga gcaataattt
     3241 tttccgcata atttgtctga ccaaagaaag ccaatttaat gactatttga acacttttca
     3301 ggctattgct cgcagtttca ctacttcggt aggaaattaa gagtgtttta taaaaagtta
     3361 taaaactata taaaaactaa agaggagcaa aaaaatggaa aaagaagggg caaaacgtat
     3421 actttgtttt ggagattccc tgacctgggg ttgggatcct gaaactcaag agcgctattc
     3481 agcaggttct cgctggacta ctatactgca aaaaaacttg ggggagagct actggataat
     3541 agaagaaggg ttaaacgggc gcaccaccat ctgggatgac cccattgaag gaaataaaaa
     3601 cgggagaaga catctcccca tattactgga atcccaccga cctctggact tattaattat
     3661 aatgctggga accaatgacc tgaaggcccg ctttcaagtt actgctcttc agatcgctca
     3721 aagcgctgga actctggtag aaatagcaaa aagaagcgat gctggaagag gaggcaattc
     3781 tccttcggtt cttcttattt ctcctccacc tttaggaaaa cttgcaatat ggcaagaaga
     3841 gtttggagag ggaacagaaa aatctcaaaa tttggctcgt tattactctt actttgccca
     3901 ggttaatcag tgccactttt ttgatgctgg agaagtagtt aaaaccagcg atatagatgg
     3961 catccactgg gaagttgctg caaaccgaca atttgccgaa gcactaacaa acaaagtaaa
     4021 ggatatcctt taagagaaaa tattagttgc ccttaaactt tctctatctt cgcaattacc
     4081 tccacacttt tctccacatt ttaggcatac cagatcatcc agtattttat tttcctcttc
     4141 cgggcgaaga tagcgttctc tcactctttt atcctgtatc cagggaagct tttttaattt
     4201 cttttcgtaa ggggtattat atacttcctg gattccttta tctcgatatt tttccctttt
     4261 tatcgccatg tttttttatt cctccccgag ataggcttta cgtacttttt cattttccag
     4321 aagttctttt ccccttcctt cgagaaccac agatccggtc tccagaacat aagcataatg
     4381 agctatctgc aaagcggctt tggcattttg ctctattaat agaatagtgg ttccgttctg
     4441 gttaatttct ctgataatat taaaaatttc cttcacgaga agtggagcta aacccagaga
     4501 gggttcatcc atcatcaaaa ggtgaggccg agacatcaaa gctctggcta ccgcgagcat
     4561 ttgttgttcc cctccactca aagtacctcc tttttgcctc actctctcct ttaaacgagg
     4621 aaaaatacga aaaatccaat ccagatcctc tttaatttcc tctttatctc gccgcgtgta
     4681 acttcctaaa agcaggtttt cccaaacagt gagattaggg aaaatcctcc ttccttccgg
     4741 gaccatagcc agccccatct ccacaatatg atgagcggat tctcgagtga tgtctatacc
     4801 ctgaaactct attttaccct gccgagctct cactaatcct gatacagtcc tcaatgtagt
     4861 actcttgcct gcaccgtttg ccccaactaa agctacaatt tttcccgccg gaacttcaat
     4921 gttaattcct ttaagagcgt gaataccacc atagaacaca tgaagatctt ctattcggag
     4981 cattaagtcg ttttccatca atcttctacc cccagataag cctcaataac ccggggatta
     5041 ttccttatct cctctggcgt accttcagct attttcattc caaaatccat cactgctatt
     5101 cgctcacaaa ttcccattac tactttcata tcatgttcaa tcagaaatat agtaagatta
     5161 aaatcgtccc gcatcttttt gataaactcc atcagttctt gagtttcttg aggattcatt
     5221 cccgctgctg gctcatctaa aagtaaaaga gagggatgag tagctaaagc tcgggcgatc
     5281 tccaaacgtc tttgttctcc atagggcaaa gattgagcct cttcttttgt aacatcagct
     5341 aatcccactg cctcgagaag gtcataagct tctctcctta tagctttttc ttccctccag
     5401 taagaaggaa gactgaacat aggagctaaa aaagagcttt gtaagcgaac atggaaagaa
     5461 accagaacat tttccaggac ggtcaaattt ccaaagagac gaatattctg aaaagtgcga
     5521 gcaatgccaa cagtggtaac taaatcgggg gaaaagccgg tgatatcttt tccctggaaa
     5581 attacccgac ccttcgtggg acgatactgc ccggtaatca ggttaaacac tgtagtttta
     5641 cctgctccat tgggcccaat taaacctacc aggtctccag agaaaagttg caaagaaaag
     5701 tcactaactg ctaccagccc gccaaaagca acagtaagat tttctaaaga aagaaggggt
     5761 tctttatttt ccactttcat ttcttccttt tatccggtca atgataagct gccaggaaat
     5821 ttctttgtca ccaaataatc cccgtcgata aaaaatcatc atgactacta aaagaacgga
     5881 aaatactacc atcctcattc ccggtattcc gggaaaagtc catgccccga tagtgatagg
     5941 agcctccact tctcgcaaaa tctcctgcaa aaaagcaaaa ataaaagcag taaccactga
     6001 ccctgtgata ctacctaatc cccccaagac gatgataatc agtaagttaa aagtcatagt
     6061 aaaagtaaaa agagaaggag aaacagtgct aattaaggtt acaaacaatc ctcctgccac
     6121 accggcaaaa aatccgctta atacaaaaga caaaagctta tgacggaaaa gattcacccc
     6181 catagcctct gctgctactt cattttctct gatggctttt aaagcccggc caaagcttga
     6241 gttcacgaga cccttaatca taaacaccga ccagaaagcc cagtttaccg tccaccacag
     6301 gttagtatat tccggtattc ctttaattcc cagagctcca ttagttaaag gaataaagtt
     6361 gttggccaga acattaatga tctccccaaa accaaaagta gcaatagcta agtaatcacc
     6421 tcgtaagcga agagtgggaa taccaatcaa taaagcagca agagcagcag ctacacctcc
     6481 taaaaaaaga gctaaaagaa aaagatgaga aggaaaagaa aaagaattaa aaggccagat
     6541 aagaggttgc aaaaaataaa cctgttcttt ttgcactaaa gggagcatta agatagctac
     6601 agtgtaacca ccaatagcca taaagccgtt gggccccaaa gaaaactgtc cggtaatccc
     6661 attaattaaa ttataggcca ctgctgctac tacgtaaata caggcggtat tcaaaatgcg
     6721 aatcaaatag gtatttagat atctctctgc taaacttact cccaacatta aaactgcaaa
     6781 aagaatcaag gttaaagcta aatctctttt ttcttctctt cccatgctac tatactttct
     6841 ctttcagggt ttcacccaga ataccactgg gtttaaccag caaaaatatg attaatatag
     6901 tgaagacaaa ggcatctcga taaccagata aaccggggaa aaaggccacg atcataattt
     6961 ctgtcaatcc aattataaaa ccgcctacca tggctcccac cacattgcca attcctccta
     7021 ctactgccgc agtaaatgct ttccagcccg gaaaagtgcc cattaagggg ttaatctgag
     7081 gatatttaca agcccacagt actccgccaa tcccggctaa tgccgaacct aaagcaaaag
     7141 tataaagaat tatcctatct atatctacgc ccatcaggcg agtggtctcc atatctttag
     7201 atacagctcg catagccatt cccattttag ttcggtaaac cagataaagc aaaacaagaa
     7261 gaactgaaac actgatgata ggcacccaga aagtcacccc ctgtatacga gcaccagcta
     7321 aaatatagac atttttcata agctccgggc gaggaaaacc tttaggacga gcaccaacag
     7381 ttaccagacc cagattttca atgaagaaag aaactcccac tgcagttata agagcagaaa
     7441 tacgaggagc atcacgtaga ggacgataag ctattctttc aatactcatt cccactatag
     7501 cacacagtgc tactcctatt attactccca gccaccaggg gagaagaaaa actcctatca
     7561 taaaaaaaga aaaataagct cccagcataa acacatcgcc gtgagcaaag tttataaatc
     7621 ccagaatgcc atagaccaga gtatagccta tggctaaaag ggcatataaa cttcctaagg
     7681 aaactccatt cattatctgc tgaaaaaaag agacccaggt catggcctaa gttatataag
     7741 taccaggtaa ctgttaaggt tacctggtac tattaaatca gtattcaata gtagtcacaa
     7801 acttgaagac accattttcc actcttttca ccactatagg tttcttgttg tctccgtcga
     7861 caatagtagc cactccagtt ataacttcca ggttttcagt atcttccatg gcatcgcgta
     7921 ttttaaccgg atctaattct cccgctcttt ttatggcatc taaaataata acataagaat
     7981 caccgcccaa agcttcaaaa gcgctgggtt ctctaccgtg tttctcccgg tataagtcga
     8041 agaacttttg tcctatttca gttatcgcct gagtggtatc agcatgagca gtgaaataaa
     8101 ccccctctac tgaatctcca ccgatgctga ccaattccgg tgcatctgcc ccatctgctc
     8161 ctaaaatcat cccttcatac cctaaatccc gggcctgctg acaaatcaga gcagtttcag
     8221 tgtaataatt agggacataa ataacatcag gattagcgtt tataatggta gtaagctgag
     8281 cggaaaagtc ctggtctcca cttctacaat tttgctcgct taccacttgt cctccttttt
     8341 ctataaaagc atctttaaag aagttgccca aagctacaca atagtcttga gcaatatcag
     8401 ataaaatagc tgcggtctgg gcttgaagct cactacgggc aaactgggca gctgctttag
     8461 cctgatcatc atcccggaag caagcccgga atacaaattt tttcccctga gtaaccagag
     8521 gactggtagc actggcagaa ataaccggaa tctgtttttc ttcacatatt gggcctactg
     8581 ctaacatact accacttatt gccggaccaa ccataactac cactttttca taatctatca
     8641 agcgagtggc agcattggcc gactctaccc ggtcactctt attatccact aaaaccagct
     8701 ccactttttt ccccagaact tcatctccta ctacttcttt agcaatgttt accccttccc
     8761 aaatcatctg tccataagct gctactcctc cagtaatttc caggttagct ccaattttca
     8821 ctgtttcttg agctaacact attccggaaa aactcagaac taaaaggaaa actgtgaata
     8881 aactgattaa tcttctcaat tctaaaatcc ccctttcaag aattttggta aaaacttgcc
     8941 ataaaattac cattgcaccg gctccttgtc aagaaaaatc ggactccata tgctacagaa
     9001 agtggaaaaa atgtcaaaat atttgaaata cgagataatt aaagccggtt tttggtcaat
     9061 ttcatatttc atgtatcaac gttctatcag taaaattttt gctttttctt aagcttcctc
     9121 tcttaatttc ccgagaattt agcctttact tctactattc cgtattttca cctccggcgg
     9181 ccaaactcag gtaaatactt ttccattcaa agaaaaggga aattttcctt atttcacccc
     9241 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacattttat tatagtagag
     9361 ccaccgaaac cttgagttta tatattacca tgataaaatt atataaacct accaaattta
     9421 ttagcagtaa acaaagattg atattagaag agaggaaaat aaaaacttat gctttttctt
     9481 tctcactttt tagctgattt ttttgcctct ttcccttcac cgctcagccc ctattttata
     9541 ggagtatatg gagttgattc tcgaactata gctatggtta tcgctaccct atctcttttg
     9601 agctctctca gccagatttt ctttgctacc cgacctctgt ttcaaaaatc agaagtacaa
     9661 ttttatattt ccctttctct tttaatatta ccctggtctc tcattccttt ttcttctcac
     9721 atctataccc tcttccttat ccttgttgta gcttttttag ccaacgcttc ttttcatcct
     9781 caaggtgcgg ctctctctgg tgaaagtggc tctcaaggga taactttgtt tgtctctgga
     9841 ggcattgctg gagctgccct aggccctatt ttcatcacct ggatagtgag cagtggaggc
     9901 atatctggaa tagtgcctct aactttaggg atctggatat gctggactat tctgtttttg
     9961 ataatcttga gaaaaagaga ttataatata tcttcctcta ttcccaggca aaaattctcc
    10021 cttcagtttt ttcttccctt gctgcctatc tggataatgg tgagcatccg tacttttttc
    10081 actgatattt tccatacttt tgtccccatt tatttaaatt ttcaaggata tagtttagta
    10141 atgggaggga tgttactctc tggaggagta atgtccggac ttttagctaa ctcgatagga
    10201 agtagaataa ggacacagtt caataatcga ttagtaaatt tgctatcttt tggagggtta
    10261 ggtgtggcta ttttaatttt tacccgggcg gaaggaatta tttccctcac cctgtcttac
    10321 ctcctggctg acttttcctc atttttgagc atgagcacca acgtctgcga agctcaagac
    10381 ctatttcctc aacatcgtgc ctttgcttct tcagtagcta tgggtctatc ctgggctttt
    10441 ggacattttc tgcatcttct ttattcctct ctactgggta atcgagtaac tctggtgatt
    10501 gagagcgctg gctttttctc tttaggaatg actgtctttc ttcttctttt tccttctttc
    10561 tttcaaagaa aagcctactg cggggttgag agtagttgag aatattacac aactggtcaa
    10621 aaaaaagggg ttctacaaac ttttttatat tcttaagcat tgctttcgct tctaatttta
    10681 tttttgcctt aacctcagtt ctcttttcca tctttgccac agatatgggt ttttctcccc
    10741 cggaagttgg tttggctata tctgctttta ccgcagggtc tttaataggt gctcccttat
    10801 gggggaaagt ggtagacaaa tttagcaaaa gaaaagtaac tcttttgatc agtcttttag
    10861 gacaagctct tttttgcctt ctcattcccc tgatgcaatg tttattgggt ctagtaatag
    10921 tgcgattttt ctttggattc tttatggtag ctcaggcttt gacccttaat gagttaacag
    10981 tcaaaataga ggaggaagaa aggagaagta aagagatatc agctatcaat gttgcccgag
    11041 cagcaggcta ctccttaggt tgtcttttaa gcggattttt aggagacctt catcctaact
    11101 ccagtttcta tttaggaagc tcggtggtca ttctgatttt cttccttact ttttatcttc
    11161 aggaggaaac gggcaaagcc gtccccttct ctcaggaaga taatcccgcc aaacactgga
    11221 ttttgcaaaa aagagttgct cttttttact tctccatttt tcttcgttct accgcagtca
    11281 acggattatc ttatttctta ccccttttct ggaaaaatat gggtcaaacc actgctctca
    11341 ttggctcggt cattgctatt tctaatttag cccaattatt tttcttcccc ctgagcggaa
    11401 aactaagctc ccggtcagat cagatggctt tccgatccac ccgctttggg cttttactaa
    11461 ccgttattcc tttccttatt gctccttttt ttcatcaagg agtattaatc cttcttcctc
    11521 agtgtatttt gagtatcagc tgggcattct tttacatcgg gactattctt tccctccgct
    11581 ctttcgttcc tctacagaaa caaggagaag ctttaggatg gttagaagta gccctgaact
    11641 taggaggcat agtgggcccc aatctttttg ctttttctct ctcctctttt aacaataact
    11701 ttccctctgc ttttttctct ctttcttttt taccgctcat agctttcttt atttccagga
    11761 aaaacgaaaa gaactttctt tcctgattga aactcaagaa gaggaaacca gagccgattt
    11821 cactttcacc cccgggatac tccctaattt accagttaaa gctcccagct cgtctgtgct
    11881 accatctaca attaaagcaa ttaccgagac attcctttct cgataaggaa tacccattct
    11941 ccccacgatt agctctccaa actctcccag aatttcattc acccgctcag cctgcttttc
    12001 tcgatcggta actattatcc ctaccactcc tactctatta tctatcatat agaactccta
    12061 atttttcatt gatttctctc aagtcttttt ccacctccac atacactcta aaccattttt
    12121 ctaagctttc ttgccactcc ttttgaggtt caaacacttc tctaacgcgg aaagtttttt
    12181 gaaaagcttc ttcaaaatct ttatagactc cggcacctaa accagccaaa agagcagcac
    12241 cccggcaact tccttctcta tcccggggaa cagggatatt tttatccaga gtagtggctt
    12301 ttaactccat ccatactcta ttttgagttc ctcctcctac attagcggtt tcttttattc
    12361 gaaaacctaa atttttttcc attttctccc agatatttcg atattcaaaa cataaaccca
    12421 aaagaagacc caggattaat tcttctcttt ctgcaccccc tgtgagattc aaaaatgctc
    12481 cctttgctcc ctcataatct ccccccagaa gataagggaa aaagatagga acacgaggag
    12541 aaaaatgagg agaaagattt tccagaaagg cataatcttc ctttaataat ctcaatatcc
    12601 aatcaacagc gtagccccca ctataaatcc cgttcatggc atagtaatag gggtaagcca
    12661 catgatgtcc ccaggagaaa ggcttttctt ctccagatgg agaaagagaa ggcaaatttt
    12721 ccagggccat ggttagagac tcagtggttc cagtagagat taaagctctc ccctctctgt
    12781 atacccccac cgaaagagcg gcacagagat ggtcatgccc cccggttact accagtgtat
    12841 caggagataa gccacagtct cttgctactt cctctctcac tgttcccaaa actacccctg
    12901 agggataaag aggcgccata tcttttgctg ataatcctgc tcgtgctaac atttcttccc
    12961 accagcatct ttctttctgt cggaaaagca tggtacgaga agccagggaa taatcaaaag
    13021 aaggaatccc gctaaagtag aagagaataa aaccagacat agaagtccag aatttggttt
    13081 tctcaaaagc ctctttttct ctttctcgat gccagaacaa cttatagaaa gaatatatat
    13141 ggtagggata aaggccgcta atccgataaa cttcttcttt gtctaattct atttcccgaa
    13201 agtatttttc tgtgcgatta tcgtaccagg gaatacttcc acccaccggt tctccatttt
    13261 cacccacttt aaccagtact tcagcataac tggatacact gagagctata atttctcgcc
    13321 ttagttcccc ggagaattgg ctgatagcct gtaaaatttg gtgtagaatt ttctgggggt
    13381 gatataactc tccctcctca tcaggaaaaa gaggagtagg aaagcttctc tcctccttta
    13441 tctctccatc gtggttataa acccgcaatt tagtattagt ggttcctaca tctatcccta
    13501 aaagcaaagc cactttatcc tccaactttc gcttctaatt ctttttcttc cagaacgagt
    13561 tcatttccca cccatatcaa acacttggta ggacacttct ctatacaaac cccgcataaa
    13621 ttgcatttat cccggtctat ttttattctt cctccctcca aagttatagc tccctccggg
    13681 caagacttca cacatatact gcattttata catcccttct tacaagactg cctggtctcc
    13741 cggggggaag ccagagagac acaagcttct accacttgtt ctcgagcagg aagaagggta
    13801 ataatagaac gaggacactc ctccacacat tttccacaac cagtgcattt atcctcaata
    13861 attatgggta atcccttttc ttcgtccata atcatagctt caaaaggaca aactcttaca
    13921 cagtttccaa aacctagaca tccatagcgg caacttttaa aagaattacc cagaaggtga
    13981 gcagctttac aatctttcaa tccctggtaa actgctacct ctcctgctac ttctcttgcc
    14041 ccttgacaga gaagaacagc tttctgcgct tcccgatgag agtcaacttc tttccccact
    14101 aaagcggaca gctcagccca cacttttggc cctcctgcgg ggcaggcatc aggaggagct
    14161 tctccggcaa caattttctc tgccagatta gaacaccctg gaaagccaca agctccacaa
    14221 ttagctccgg gaagaatttc ctctgccttt tgcaccagag ggttgacttc tactgcaaac
    14281 ttttgagaaa accaagccag cattactcca aaaatcaggc tcaaaacccc aataagaaca
    14341 gtgggcatta gaatatccat atctacctcc tatttcacca ggcccttaaa gcccataaaa
    14401 gcgatagcta aaagacaagt ggaaataaaa actatgggat aaccctggta aaactgcgga
    14461 actttagaaa atctcagttt ttccctgatt ccagctaaaa gcagcaaagc tatcgtaaac
    14521 cctaaggcta cacctatact atataccact acttgaagaa gattataatt atagtctact
    14581 ccaaaaaagg taactgctaa aatagcacaa ttagtggtaa taagagggag atatataccc
    14641 atggcctggt ataagttagg caccgttttc ttgagaatcg cttccagtaa ttgcactaaa
    14701 acagcaatta ctagaataaa caccataatc tggagatatt ccaaccccag aggcacaaaa
    14761 ataaagttcc acaaagccca agtagctata gaagctaaaa ccatgacaaa ggtaactgcc
    14821 atccccatcc ccagggaatt cgccacatta gaagacatcc caatgtaagg acatagcccg
    14881 atatagcgag ctaaaatgat attatctacc aaggcagcac tgataaaaat tttgaaaagg
    14941 tccatagcac tcatagatta tccctccgat ggaaatgatt tagcaaagca atgtaaatac
    15001 caataagaag gaaagctccg gggggaagaa tcataatcat catgccctgg taagaagaag
    15061 gaaatatttg gtggtaaaaa aattgcccgt agccgaaaag ttctctcacc acaccaatgg
    15121 ctgcgattac taaagtgtac cctaccccca ttcccagaga atcagctatc gtcaatatca
    15181 ctggctttcg agaggcaaaa gcttctgccc gagccataat aatacagtta actacaatca
    15241 gaggaatgaa aacccctaaa gcctcagaaa gggcagggag ataggctttc atcaccagat
    15301 ccacaatagt agtaaaagta gaaattacca caatgaagat gggaatacga acttcattgg
    15361 gaacaaaatt tcttatcgcc gatatcacca ggttggatcc ggttaaaaca aaaataactg
    15421 ccgctcccat aaagaggcag ttttccagcc tcaccgatac tgctaatact gagcataaac
    15481 caatcatcag gcgtaaaacg gggttttctc ccacaatgcc atttttaaaa caatcccaaa
    15541 actccttcat tgctctctca cctcctccca gtaacttgct aaaccctgac aagcccggac
    15601 tatagcccgg gtagaaatag tggctccact tatagcctca atatctctgt tgatttcgaa
    15661 gggatcaatt aaagacttac caataaattg atttagaaaa tcagcgcttt caatattaga
    15721 acccagtcct ggagtttctt gatgttctaa tacttccatt cccgtaagtt ttccttcctc
    15781 agaaatccct accaaaagta taattggtcc gccatatcct gaagtgctca ctcgaaacac
    15841 caacccttcc ctctttcctt cttgtagtgc ttcataaact tctaaaatgg aaaggtcttt
    15901 gatagaatct tgtgcttcgg gcagagtttc ccactttttg aactcttgag caaaaggagc
    15961 aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaaagac gaatgacccc
    16081 cgtaatagct ccggctccca gaccaaaaat atagcgacct tttgagctaa taggagaagt
    16141 tacataatct gtagccataa agcacgctcc catgattgct cccccgctta agatatgaaa
    16201 aagagcgctt tgccctccca gaatagccac acaagagaga ccaacaaaat aagaaagggg
    16261 aatatcccag tgcaccaccc ctcgccacca cagatataaa aatccgatga ggagaagaag
    16321 cgcagaaatc tctccaatag aaccaggaac ctgccccacg aaaagacgcc acaaaatacg
    16381 aggatctgct tgatgcaatg aactatagcc atacatttga gcaatactga gaggtgtagg
    16441 agtggtaacc atctccactc ccgagaaagt tgaagcagca ccgcctccgg agaagccaga
    16501 aaaaccaaaa ggagcgctca cccaccaggt agacatagcc ttcggccagg aaaccaacaa
    16561 aactgctctt cctaccaaag cggggttaaa tatattttgc cccagacctc caaacatttc
    16621 tttccccagg aaaatagccg cccctcctcc caccagaggt aaccagaaag gcgcggaagg
    16681 aggaagagat aagcccagaa gcaaaccggt aatcacagca cttccatcag taatagagag
    16741 aggtttctta aaaatcttac aattgagagc ttcaaatcct attgtgctcc ccacagataa
    16801 aagaatagtt aacaaagtag aggaaactcc aaaaaaatag attccccaga tagtagcagg
    16861 tagaagcgcc aatactactt gcctcattat ccagggaaca gtggtctcgt ctcggagatg
    16921 aggcgaagaa gtgataacca attttaattc ttcattcatt gatttccccc ctttctggaa
    16981 cgacgcataa cttctgcttt agctaacctg ataagatgag taagagaaat gttggaggga
    17041 caaacgtaag aacaggctcc acattccata caatccagca cccgctggtc tctggcctca
    17101 tcaaaagcct gatactcaga aaatttgccc agcattgtgg gaaaaaggcg cataggacag
    17161 gcatctatgc atctccggca acgaatacaa ggatattcct ttttttcata gagattgctc
    17221 aaaaagacaa acccagaagt tcctttcacc actggaactt gcagagaggt ttgtgctacc
    17281 cccatcatcg gtcctcccat aatcactttt ttaggagttt ccacccatcc tccacaaaaa
    17341 tccacgaggt ccgataaaac agttcccaga ggtacttgaa ggttttctgg acttttaaga
    17401 cactctccgc ttaaagttat aacccgctct ataaggggaa gaccttcttc agccatcctt
    17461 cccactgccc aggcagtttg cacattgttt accaccactc ccacatcgag gggtaatcct
    17521 ccaggaggaa cttctcttcc cagaatagca acaatcaact gtttttctga accctgagga
    17581 tatttagtaa gaagaggaac tactcgagcg gggaaaggaa ggtcctttac taatttatca
    17641 aatttttcta tagcctcaat tttattttct tcgattccta taaaaactct ttctgcccca
    17701 caagccctca ccagaactcg caagccggtc aaaatagcag atgatctttc tatcatcacc
    17761 cggtaatcgc aggttagata aggttcacat tctgccccgt ttaaaataaa agtgtcaatt
    17821 tttttacctg gaggaggaga aaccttcaca tgggtgggga aagctgcccc ccccaaaccc
    17881 accatccctc cctctctaat ccactcccgg agaaaagcgg ggtctttatt ctcaatatcg
    17941 cagagaggat cttttctttc ctcttctcct gaaccatcat tttctatggt tatagcagga
    18001 aatttactca atagagggtg gaaataatct tctacccctt ctattttacc agccaaggga
    18061 gaatgcaggg gagcagaaat aaaagcttct acatcagcaa ttttctgtcc tctttttacc
    18121 ctatcacctt tttttactaa aggtgatagg ggagcaccag tgtgctgaag aaggggtaaa
    18181 accacccgcg aaggaaaagg aaattcttta ataggttttg tggcagtaaa ttctttaaaa
    18241 gtgggggagt ggatcccccc agggaaagta ttaagcctca taaatttact cctcctttaa
    18301 tattttaata ttcctccaga ttttcctcta ttaatttggt atgtaaatta ccggtcataa
    18361 agtctcggtt ttgcaaaatt ttcatatgga agggaatagt agtggaaaca ccttctaccg
    18421 caaaagaatg caaagcttct cgagccctgc ggatggcaaa atcccgatca caatcccata
    18481 ctatcaattt agctaaaaga gaatcataat aaggaggaac ttcgtaaccg gagtagcagt
    18541 gagaatctac cctgattcct ggccctcgag gaacatcaaa tcgagaaatt ttgccgggag
    18601 aaggagaaaa gttatgggcc acatcttctg catttattct catctccaga gcatgtcctc
    18661 gtaaagaaac ttcttccggg gaaaaagaca gcttctcccc tgctgcaagg caaatctgct
    18721 cccttactaa atctatccct gaaaccattt ctgtgatcgg atgttctact tgaatacgag
    18781 cgtttaattc catgaaataa aaagaaccgt cttcagttac cagaaactct actgttccgc
    18841 aagtggtgta gttcagcact tcagctactt ttattgctgc ctctcgcaat ttctctctcg
    18901 tctcctcagt caagaagggg gcaggagctt cttctaaaat tttctgttgc cttctttgta
    18961 gagaacattc tctttctccc agatgagcca ctcttccttc cttatctctt acaatttgca
    19021 cttccacgtg cctcgcctta gtcaagagct tttccatata cacttcttcg ctgccaaaag
    19081 ctaattttcc ctcccttttc gcactttcta aagcccctgc cagttgacca ggctctctta
    19141 ccaccctgat tcctctccct ccccctcctc gagaagcctt gagaaaaaga ggataaccta
    19201 tttcttctgc tatttttctt gcctcattct catcttttaa gggctcccta gagccaggaa
    19261 ccaccggtat tccagcttct tctagaattc ttttagcctg taatttatct tttatccgtt
    19321 ccagcaaatc cgcgtccggc ccaataaaag taataccttt ttcttctaat ttcctagcaa
    19381 atgcaccgtt ttcagataga aaaccgtaac caggatggat agcatcaacc tcagttaact
    19441 cacaagcacg aataattttc tcttcgttca aataggtgtc ttgaggtaaa cttcctccta
    19501 aagctacttt ctggtctaca aactttaaat gtagaagatt ataatccggg tcagaaaata
    19561 cccccactgt tttgattccc agctctctgc aggctcgcac tacccggaga gcaatttctc
    19621 ctcgattggc tatcaatatt tttccgaaca aagttttaac ctcctaaata agaatcctat
    19681 cttccggata agtgattaaa tctttttccc gggagggccg caacattacg agagtcataa
    19741 tccccactct tcccacatac atagtgatga taagcaaaaa tttactgaga tctaaaagac
    19801 cggctgttat tcctcgagaa agacccactg ttccgaaggc agaaaatacc tcaaaacaaa
    19861 tgtccagaaa aggagcgggt tgaataacca atagaaccat aatcactcca aaaaccagga
    19921 agaaggctaa aaccaccacg cttatagccc ggaataccgt tgcaggcgct actcggcgat
    19981 ggaaaaattc cactttatct ccccctctta ctatattcat tactaccccc aaaagcaagg
    20041 caaaagtggt agttttcacc cctccccctg ttcctcccgg agaagcacca ataaacataa
    20101 gtaacattat aagaaaagca gaagcttctc gcaaaccagc agtaggaaca gtattaaaac
    20161 cagcagtgcg gggagtcaca gcttgaaaaa gaccggccat tattttcccc cccgggggaa
    20221 gttcagccag agtggaagga ttaccccatt ctaaaagtaa aattaaaatg gtccctagag
    20281 aaattaaaaa taaagtagtc ctcactacca ccttagaatg aaaactcgac aaaccacgct
    20341 tttccactat ttctcgaagt accacaaaac cgaggcctcc cagtattatg agtcccataa
    20401 ttgtaaaatt aactactgga tccagaacaa aaccctccag attgctactg aatattgaaa
    20461 aaccagcatt acaaaaagca gatatagagt gaaaaagagc aggaaaaata gcttcccctg
    20521 gtgtaaagta acgcaaaaag ttaagaaaga gaatcataaa acctattcct tcgattaaaa
    20581 aagtatattt caaaacggaa agaacaaaac gtaccactcc tcctggaaaa tccaggctaa
    20641 aagagtcacg aatagctaat cgagttcgat attcaatcct ccgccggaga gctaaggcta
    20701 aaatagtatt taaggtcata ta
//
LOCUS       DAOPSS010000013        20097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2272_length_20097_cov_8.332152, whole genome shotgun sequence.
ACCESSION   DAOPSS010000013 DAOPSS010000000
VERSION     DAOPSS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 20097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20097
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2272_length_20097_cov_8.332152"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1063)
                     /locus_tag="PKN31_01655"
     CDS             complement(41..1063)
                     /locus_tag="PKN31_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA98668.1"
                     /translation="MAQISLGKTGLYVEQNGFGALPIQRVSKQDAVYLLRKAYDGGMN
                     FYDTARSYSDSEEKLGEAFSGMWDKIVIATKIPAVDVPRFWDDLHTSLRMLRRDHIDI
                     FQFHNPPISPRPGDDGGLYDAMLEAKQEGKIRFIGMTNHRLDVAWEAINSGLYDTLQF
                     PLSYLSTDKELELVQACKEKEIGFIAMKALSGGLITDSAAACAWLSRFGHVVPIWGIQ
                     REGELDEFLSYIPIPPALSDEMMDRIEADRQELHGNFCRGCGYCMPCPQGISINMCAR
                     ASLLLRRAPSEIILSEDGQQMMRKIEDCTECGQCSEKCPYGLDVPDLLKKNYEDVKEV
                     LAGKPI"
     gene            1374..2360
                     /locus_tag="PKN31_01660"
     CDS             1374..2360
                     /locus_tag="PKN31_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA98669.1"
                     /translation="MKKRKLGNSGLEVSAIGFGCMGMSQSYPPFPEKGEAISLLRSAV
                     ELGVTFFDTAEVYGPYTNEELVGEALEPYRDKVVIATKFGYHIQNGKSAGLDSRPETI
                     REAVEGSLKRLRTDHIDLLYQHRVDPNVPIEEVAETVGQLMKEGKVLYWGLSEAGAQT
                     VRRANAVLPLAAVQSEYSMWYRKPEEGLFPALEELGVGFVAFSPLGKGFLTGRFNKDT
                     RFDSTDFRSSIPRFKPENLEVNQDLLELIKDIAAKKNATTAQIALAWVLAQKPWIVPI
                     PGTKKLERLKENLGAQDIEFSAQELAEINEALSKINIAGERYPEEVEKLTGK"
     gene            2381..2869
                     /locus_tag="PKN31_01665"
     CDS             2381..2869
                     /locus_tag="PKN31_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOA98670.1"
                     /translation="MRGNILIAYYSRSGNTRKLAQLIHQKVGGTVHEIQPEIPYPASY
                     NATVEQAKKEIQAGFRPPLKTKIEHIEAYDTIFLGSPNWWSTIAPPVATFLEENDLSG
                     KAIIPFCTHGGGGRGRIERDIAKLCPQSTVMPALAIYGSGGKDAEAQVSAWLKSMGFT
                     EK"
     gene            complement(2993..4354)
                     /gene="rlmD"
                     /locus_tag="PKN31_01670"
     CDS             complement(2993..4354)
                     /gene="rlmD"
                     /locus_tag="PKN31_01670"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOA98671.1"
                     /translation="MDKGQIIEGKVVDFALPEGQGVLKEGGRVVFVPGVLIDEVCRVR
                     ITKLKKNYALGEVIDILEPSPSRVEPICPHFLEGCGGCRFQFMDYEEQVKAKERNARQ
                     TLEKVGGISWEEVDYEGFIPSPSPWEYRNKMEFNFGEKEGELILGLRPRGRYWDLVDL
                     KVCYLMKKDLTQTLLKLFRDYGRNSSLSGYDPRKKEGVLRNLLVRYSSSQELLLGLST
                     VSVDLPRQEDLIEELRKNFPSLEGLVHIINNSPASALIFEDKKVLWGKDHFFERVGSV
                     NYKVSIESFFQVNSFLSSTLYEKAAHYASLSGGETVLDLYCGSGGVGLFVADSAQQVV
                     GVEENPQAVEDAKFNAWLNGRNNFTCIPGRVDKVLPLFSNVGVDVVIVDPPRAGLDKK
                     VVRRVSSLSPQRLVYISCNISTFARDLLLFREQGYHLEKITFLDLFPQTPYFESVALL
                     KKR"
     gene            complement(4354..5100)
                     /gene="rlmB"
                     /locus_tag="PKN31_01675"
     CDS             complement(4354..5100)
                     /gene="rlmB"
                     /locus_tag="PKN31_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOA98672.1"
                     /translation="MKEELIYGRQPVREALKSEKRKVKRLFVMEGAQGKIIEDIKQLS
                     QKRKIPWEIKDKTFLEELVGKNVNHQGVVLLAQPRASLTWQEILKKNKTSPEATVVFV
                     DRVEDPANLGTIARTAAFFGVEGILISKARSAPLDSPVVRASAGGIESIDIAQINNFT
                     QVIKEFQKAGFWIVALEADGDKDIWDADLRGMPLGLVVGGEHEGIRRVVRSSCDMVLR
                     IPSRGVINSLNVAVALGIGIYEVMRQRSTN"
     gene            complement(5097..6521)
                     /gene="cysS"
                     /locus_tag="PKN31_01680"
     CDS             complement(5097..6521)
                     /gene="cysS"
                     /locus_tag="PKN31_01680"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOA98673.1"
                     /translation="METQLFNTLTQKKETFFPQEGDKVRFYVCGPTVYDLIHIGNARV
                     FVVFDSLRRFLEHRGWEVIMVQNFTDIDDKIIKRAEELGESPQELAERYIAEYWHDAE
                     NLKIRKATFHPRATEHIEDIIALIKILEEKGYTYEKEGDVLFDVSRFPSYGKLSRKNL
                     DELVEGARVEARYEKKNPADFVLWKKAKPGEPSWESPWGEGRPGWHIECSAMSMKYLG
                     PTLDIHAGGIDLIFPHHENEIAQSEAATGKPFARFFLHNGYVNIDQEKMSKSLGNIVT
                     VRELTQKYEPSVLRLAIFSAHYRSPINLDENLLQSSRQFEERIKNFLRNIGFIEEKIK
                     DNSALLKEDKEEYHKIDEFSRSFQEALADDFNTPQALGVLGEFLRFFNQYLSPRRGKW
                     DVQVLEKIKDFLKMVEEILGILPGKEMEISDEAIKKLVEERERARRDKDWERADQLRE
                     EIKSRGYVVEDTPLGPRFFKMEEQ"
     gene            complement(6502..7947)
                     /gene="gltX"
                     /locus_tag="PKN31_01685"
     CDS             complement(6502..7947)
                     /gene="gltX"
                     /locus_tag="PKN31_01685"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOA98674.1"
                     /translation="MGRVRTRFAPSPTGFLHLGGARTAAFNWALARKEAGDFILRIED
                     TDRERSKKEFEEAILEDLRWLGIEWDEGPDRGGPYAPYRQSERGEIYQSFIARLKAEN
                     LIYPCYCTVEELEEERKKALKMGKAPRYSGRCFFLSPEERKEKEERGIKPSWRFRVPR
                     GKEIVLEDLVRGKVVFHTNSLGDFIVLKSDGVPTYNFACVIDDALMDISHVLRGEEHL
                     SNTPYQILLYQALELTPPLFAHVPIILDEERTKLSKRRGEVSVRFFREEGFLPEAILN
                     HLLTLGHSFPDGREIVEGEELYTLFSLERIGKGGAIFDLSHLNWWNKTYIRRMDIREL
                     EEKLTPWGGEEIREFKKELGEERFYLLLDLVREESVTLKEIAERLRDYISVKEEKVED
                     EEKLQVLKFLLSSLNDFPVPWEEEEVKKNLRRWQKESGFPPSIFYPVLRWALIGAEEG
                     PELDRLLVALGREKVLEKIKAKVGDNGNSTV"
     gene            complement(7935..8684)
                     /locus_tag="PKN31_01690"
     CDS             complement(7935..8684)
                     /locus_tag="PKN31_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOA98675.1"
                     /translation="MFCCKPFAEERINKILALVICGLVITAPATESAAEELISLESQS
                     HYCSINMEGRTILRIRYGGGYSTLEERAKVIAQSLAKLFKNSQKIVNFHVVWEKDKAT
                     AYAGQHKLFTVLREDALVNKSDPLSLALSWLNNVQIEFYRVKEEKEVKEIAEGIASWC
                     SNGFSGRATASGEIYTPYAFTAAHRTFPIGSVLLVTNLEDGREVVVRVNDRGPWREDR
                     VVDLSLAAAKVLDIHEDGLSKVKVEVLEWGG"
     gene            complement(8636..9133)
                     /gene="ispF"
                     /locus_tag="PKN31_01695"
     CDS             complement(8636..9133)
                     /gene="ispF"
                     /locus_tag="PKN31_01695"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOA98676.1"
                     /translation="MSSWRVGLGYDVHPLKEGRKLFLAGVHIPYELGLHGHSDGDVLC
                     HALMDSLLGAAGLQDIGYWFPPTSPSTQGVRSTLLLEEVCKKIREKGWEIENVDTTLI
                     AEQPLIAPFVGEMKKTLSLTMGVSEEAIGIKATTNEGLGSLGKGEGIGCFAVSLLRKK
                     ELTKF"
     gene            complement(9130..9822)
                     /gene="ispD"
                     /locus_tag="PKN31_01700"
     CDS             complement(9130..9822)
                     /gene="ispD"
                     /locus_tag="PKN31_01700"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOA98677.1"
                     /translation="MFFAIIVAAGKGERVNSVVPKQYLPLLGKPVLAHTLDVFERSPW
                     IGEIIVVINSLHEEMFKKEVWEKYGYRKIKKCVSGGETRQDSVYCGVRELKDRGADFV
                     LIHDGVRPLVDDKILARCVEAVVKFEAACCALPCADTIKLSLDGEIIDETLNRQKIWR
                     AQTPQAFRISLIWEALEKAKEEGFQGTDDSALVERMGKPVRLVLGSEDNLKITTPQDL
                     ILAEELLRWRKK"
     gene            complement(9849..10814)
                     /locus_tag="PKN31_01705"
     CDS             complement(9849..10814)
                     /locus_tag="PKN31_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOA98678.1"
                     /translation="MSKSGWLILALIVFVLILFYFLSRKKENSFLGLFESLAQFFNHL
                     SAIDVLVALGGLVVGLVIGVLLTYPISFLPFKNPSLPLAVTLICGVLGFAVFMWRKED
                     IVTIFTGIPRLKPPRGGGNSAKILDTSAIVDGRIADICKTGFVEGDLLIPRFVLRELQ
                     QVADSQDPLKRQRGRRGLDILNRMRKEKKVNVKIIDRDFPEIRDVDAKLIKLAKVLEA
                     KVITNDFNLNKIAELEGVEVLNINDLSNALKPYVLPGEELRVQVIREGKEENQGVGYL
                     DDGTMVVVEEGKKYINTTIDTVVTSVLQTPAGRMIFAKLKGSGRS"
     gene            complement(11536..12897)
                     /gene="radA"
                     /locus_tag="PKN31_01710"
     CDS             complement(11536..12897)
                     /gene="radA"
                     /locus_tag="PKN31_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOA98679.1"
                     /translation="MNYCCSNCGYSTPRWLGKCPQCGEWNSFLPVSREETKKERSSSY
                     SPPLSLAEVKEKKFQLGDRFSTSLKEVDRVLGGGLVEGSLILLSGDPGIGKSTLLLKI
                     AEGIAREGKKVLYASSEESLYQVYLRAERLGLDCNPPLFLLSTNSFEEIREGISSLQP
                     QAVIVDSIQNLGRENLEFLPGSVATLRDLSSRFMEIAKKENIVLFLVGHVTKEGAIAG
                     PKALEHLVDVVLYLEGDANHPLRLLRSMKNRFGSTQEVGILEMQERGLVEVKDPSHYF
                     MEESKLAGIGTARTVWAEGKRAMVVEIQSLVHPSYFDFPRRVSLGMDLNRLHLLLAII
                     EKNTRLRFSRQDVYLSMVGGIKTSETALDLPVCFSLVSSRLNREIDKETVYLGEVGLS
                     GEIRPVPLLEQRINESARMGIKRVVVSSYHSRDFLSERWGEIALVGYSNVREALEKEG
                     LIG"
     gene            complement(12907..15372)
                     /locus_tag="PKN31_01715"
     CDS             complement(12907..15372)
                     /locus_tag="PKN31_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOA98680.1"
                     /translation="MFERYTERARKVIILAQDEAVRLKHNHIGTEHLLLGLLREREGI
                     AAKILDSFDITMDLVRTELENFVDQREYQGSREVAFTSRAKRVLELALDETRRLGHNY
                     VGTEHILLGILREGEGIGAQILQRLGLDLEMVRAKLYEILSENPDEVGGLSETSKPSQ
                     ESRTPVLDEFSRDLTRLAIENKLDPVIGREKEIERVVQILSRRTKNNPVLLGEPGVGK
                     TAIVEGLAQKIVKGDIPEILKGKRVVSLDLAAIVAGTKYRGEFEKRLKKIVSEIVQSK
                     EVILFIDEIHTLVGAGAAEGAIDAANILKPALARGELQTIGATTTMEYRKYIEKDSAL
                     ERRFQPVYVDEPTAEVSVEILRGLKERYEEHHRVKISDQAVEAAVKLSQRYIADRYLP
                     DKAIDVMDEASSRVKLQATILPESLKELEKEAEEIKKLKEKAVKNQDFEEAAHYRDEE
                     EKLKKKYRDRKENWLKEVDSSRPLVGEEDIATVVSIWTGIPISRLDTEEKEKLVNMEK
                     EIHERIVGQEEAVEAVCRAIRRSRAGLKDPNRPISSFIFLGPSGVGKTELAKVLAEFL
                     FGKEDNLISLDMSEYMEKFTVSRLIGSPPGYVGYEEGGQLTEKVRRKPYSVVLFDEIE
                     KAHPDVFNILLQVMEEGRLTDAQGRVVDFKNTIIIMTSNLGARLISSQSNIGFGERSE
                     KEVMSYQDIKQKVTEEVKKVFTPEFLNRVDEIIVFRPLNQKEIEAIVDLMMKETQERM
                     REHKMEIDLTPEARSLIAQEGYDPNFGARPLRRAIQKLIEDPLSDLILKGVFKEGDRI
                     LVGVEEGEIHFTKLLPLEFSLKN"
     gene            complement(15365..16396)
                     /locus_tag="PKN31_01720"
     CDS             complement(15365..16396)
                     /locus_tag="PKN31_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98681.1"
                     /translation="MSFTLGKRLFSSRKSRLLDGFNRVALVLSRNLARIPFPSSIYFS
                     PLWEEEVKERVEKAYSSLARKGFSWVEIGSHSREVEPLSWVFPQLLSDSPEKILIHKG
                     NLGAIINHQDHLSLFAEGEYRNLESYWKRLNKWDDFWESKLEYAYSEQWGYLTSSPSR
                     LGTGLKIYMRLHLPALSFLYGEKRVFQWFKDNDRFLVEVYGEDDQALAHLFVFSNLFT
                     LGVKETEILKMMQDFGKGIWEWEKQVRTSLVKSSSRQNVFWQMMERLYEELMEGNEEQ
                     NEKKILDFLSFWFLARDEGILPEGWGFEFMKSADLWDKIINGGLLRGQSLKVLGFLGI
                     ELGRIWNDV"
     gene            complement(16393..16878)
                     /locus_tag="PKN31_01725"
     CDS             complement(16393..16878)
                     /locus_tag="PKN31_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98682.1"
                     /translation="MKCDICGWREAKVVVYLIGGGELKNKLFLCPACSQEVSISRVVA
                     PGSYKSPPSTRFFPLKKEGGKRCSFCQWEWEDFLKGGAVGCSHCYSSYSKEMQSWLSK
                     NQLGMHHRGKIPINWQRQKKIQNAIFRLGRDLENAIEQENYEKAGHLKKVMEKLRSQL
                     K"
     gene            complement(16879..17361)
                     /locus_tag="PKN31_01730"
     CDS             complement(16879..17361)
                     /locus_tag="PKN31_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOA98683.1"
                     /translation="MYSLSDDIEQHIIKLIDGSPDQMITIQRGRLANEFDCAPSQINY
                     VLATRFNVFRGFIVESRRGGSGYVRIRRVPIDRLEPVVVYLDDNEESLRESDVEDLVS
                     WLEREEFITPREGALVRAATIDALNKVAGLDFSSVADRNRLRSRIFREILLQVLRWVE
                     "
     gene            complement(17427..18911)
                     /gene="lysS"
                     /locus_tag="PKN31_01735"
     CDS             complement(17427..18911)
                     /gene="lysS"
                     /locus_tag="PKN31_01735"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOA98684.1"
                     /translation="MDEEVTRYEENTEYEYKREKIQALKDLGIDPYNGSFCKTHDIEE
                     IKNHADNLVDKDIKVKIAGRIMALRRHGKAVFADLQDRTARIQIYFRKDIIGEDSFTL
                     LREIEVGDIIGVEGKVFRTHAGELTVLVENFTLLSKCLRPLPEKWHGLKEVEVRYRKR
                     YLDLIMNPNVRRIFYLRFQLLKELRAFLDQRGFWEVETPMMQVIPGGALARPFKTFHN
                     ALGMELYLRIAPELFLKRLIVGGLEKVYEINRSFRNEGLSTRHNPEFTMLELYQAYAD
                     YQVMMELTEEMLSHVVKKITGSYEIEYQSMVLDFTPPWHRISLPQVIQELVGVDIVED
                     SLETIKAKAKERGVAYEDNWDRGKFVNEFLEKFVQPRLVNPTFVFDYPVEISPLAKAK
                     KDNPQLAERFELFIGKEEIGNAYSELNDPFEQKKRFADQLRKRDEGDLEAHLIDEDYI
                     EALEYGMPPTGGLGIGIDRLMMLITNSSSIREVILFPLLRPSSD"
     gene            complement(18918..19415)
                     /gene="greA"
                     /locus_tag="PKN31_01740"
     CDS             complement(18918..19415)
                     /gene="greA"
                     /locus_tag="PKN31_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOA98685.1"
                     /translation="MEKKKDDGKIVLTREGYKKLEEELKYLKTVKRREIADKISHALS
                     FGDLSENAEYEEAKNEQAFIEGKILSIEEKLRKAEIVDTEGGNTDRVTLGSRVILENL
                     DQNKKIEYLIVDSVEADPSARKISFESPLAQALLNKKEGEEVEIKVPAGVVRYRIIEV
                     KGKDE"
BASE COUNT         5748 a         5017 c         3499 g         5833 t
ORIGIN      
        1 tccagaaacc ctcaaagagc taaaatgaat cctttatcat ttaaatgggc tttcctgcca
       61 gcacttcttt aacatcctca taattctttt ttagcaaatc aggaacatcc agaccataag
      121 gacatttttc agaacattga ccacattctg tacaatcctc aatttttctc atcatctgct
      181 gaccatcttc agataaaatt atctcagaag gcgcccgcct gagaagcaac gatgctctgg
      241 cacacatgtt aatggagatt ccttgcggac agggcataca atatccgcag ccccggcaga
      301 aattaccatg caattcctgg cgatctgcct caatcctatc catcatttca tcgctcagag
      361 cgggaggaat gggaatataa gagagaaatt cgtccagctc cccttcccgc tggatacccc
      421 agataggtac aacatgccca aagcgggaca accatgcaca ggcagctgca gaatcagtga
      481 taagccctcc cgaaagggct ttcatggcaa taaagcctat ttctttttct ttgcaggcct
      541 gaaccagctc cagttcttta tcggtgctca ggtagctgag tggaaactgg agggtgtcat
      601 ataatcctga gttaattgcc tcccacgcaa cgtcaaggcg gtggttggtc ataccaataa
      661 agcggatttt cccctcttgt tttgcctcta acattgcatc atacagcccc ccatcatcac
      721 cgggtcgggg agagatgggg ggattgtgga actggaaaat gtctatgtga tccctcctca
      781 gcatacgcag gctggtgtgt aaatcatccc agaaccgggg aacatcaaca gctggaattt
      841 tggtagcaat cacaatttta tcccacatac cagagaacgc ttcacccagc ttctcctcac
      901 tatctgaata actacgtgca gtatcgtaaa aattcatacc gccatcatat gctttgcgca
      961 gaagatatac tgcatcctgc ttagatactc tctggatagg aagcgcccca aaaccatttt
     1021 gctccacata taatccagtc tttcccagtg atatttgcgc catttttatt actcctttct
     1081 aatcagcaat ccatgcccac caatagctga caacagacga aaaagctggt agaggttata
     1141 tcttagaaat atttttaatg atttcatcaa actctttcat aaccccgggg atgtcaatct
     1201 ccgatgaatg aggattttta caactctttt agatatccta tcacttagag ttaactccat
     1261 gtcaaggata aaattttaaa agctcttgac atggagttaa ctctaaactg taaaatgact
     1321 acagactgca aaaacaagtt tataaatcta gaaatttcag gaggtgcttt accatgaaaa
     1381 aacgtaaact gggaaacagt ggtcttgaag tctcagcgat tggctttgga tgtatgggta
     1441 tgagtcagag ctatccaccg tttcctgaaa aaggggaagc gatttctcta ctccgctctg
     1501 ccgttgagct gggggtgact tttttcgata ccgccgaggt gtatggtcct tacactaacg
     1561 aagagctggt tggtgaagct ctggagcctt accgagacaa agtagtaatt gctactaaat
     1621 ttggctacca cattcaaaac gggaaatcgg ccggattgga tagccgtccg gagaccatca
     1681 gagaagcagt agaagggtct ttgaaacgcc tgagaactga ccatatcgat ttattgtatc
     1741 aacatcgtgt agacccgaac gtgcccattg aagaagtagc tgaaacagtg ggacaattga
     1801 tgaaagaagg taaggttctg tactgggggc tttctgaagc cggcgcccag accgttcgtc
     1861 gcgctaatgc agttttacca cttgctgccg ttcaaagtga gtattctatg tggtacagaa
     1921 agccagaaga aggattattt cccgctcttg aggagctggg agttggtttc gttgctttca
     1981 gtccactggg taagggcttc ttaaccggcc ggttcaataa agataccaga tttgacagta
     2041 ccgattttcg cagttcaatt cctcgtttta aaccggaaaa tctcgaggta aaccaggatt
     2101 tgctcgaact gataaaggat atagcagcaa agaaaaatgc tacaacggct cagattgcac
     2161 tggcatgggt gcttgcccaa aaaccctgga ttgttcctat tcccggtacc aaaaaactgg
     2221 aacgcctgaa agaaaatctg ggagctcaag atatagaatt ctcagcccag gaattagccg
     2281 aaatcaacga ggcgctctca aaaattaata ttgccggaga acgttacccc gaagaagtag
     2341 aaaaactgac tggaaaataa atttagaaag gaagatttgc atgagaggaa atattcttat
     2401 cgcctattat tcccgctccg ggaatacccg aaaacttgct caactgattc atcaaaaagt
     2461 gggtggaact gtgcatgaaa tccagcccga gattccctat cctgcttcat acaacgcaac
     2521 tgtggaacag gccaaaaagg aaattcaggc cggtttccga cccccactaa aaactaaaat
     2581 tgagcacatt gaagcatatg atacgatttt tttaggttct cctaactggt ggagcacaat
     2641 tgcccctcca gttgcaactt tcctggaaga aaatgattta tcgggaaaag ccatcatacc
     2701 attctgtacc catggtggag gggggagagg acggattgaa agagatattg caaagctctg
     2761 tcctcaatca acggtgatgc ccgctctcgc catctatgga agtggaggca aggatgcaga
     2821 agcacaagtg tctgcatggc tgaaaagtat gggctttact gaaaaataac cacagttaaa
     2881 gagacaggat atgagttgac attataaatc tgtttttgct ttttaatctt acggcataag
     2941 ctttttctca taaccccatg acgatagaaa agggagagag acaaaagtaa gcttatcttt
     3001 tcttcaggag agccaccgat tcaaaataag gagtttgagg aaaaaggtct aaaaaggtaa
     3061 ttttctccag atggtatccc tgttctctaa aaagcaataa atcacgagca aaagtactga
     3121 tattgcagga gatataaacc aggcgctgag gagaaagaga ggaaactctc cttaccactt
     3181 tcttatccag accggcccga ggagggtcta ctatgaccac gtccactcct acattggaaa
     3241 aaagaggaag aactttatct acccggccag ggatacaggt aaaattattc cgcccgttca
     3301 accaggcgtt aaacttggca tcttctaccg cctgggggtt ttcttccact cccaccacct
     3361 gctgagcaga atcagctaca aaaagcccca ctcctccact tccacaataa aggtctaaaa
     3421 ccgtctctcc cccgcttaag gaggcatagt gagctgcctt ttcataaagt gttgaagaaa
     3481 ggaagctatt tacctgaaaa aaactctcta tagaaacttt ataattgact gaccccaccc
     3541 gttcaaaaaa atggtctttg ccccacagaa cctttttatc ttcaaaaatc aaagcactgg
     3601 ctggtgagtt atttatgatg tgcacaagac cttcaagaga aggaaaattc tttcgcaact
     3661 cttctatcaa atcttcctgg cgagggagat ccacactgac ggttgagaga cccagaagta
     3721 gttcttgaga agaggaataa cgcaccagca agtttctcaa cactccttct ttttttcggg
     3781 ggtcataccc tgaaagagag gagtttcgcc catagtctct aaacagcttt agaagggtct
     3841 gggttaagtc ttttttcatc aagtaacaga ctttcaggtc taccaaatcc cagtatcgcc
     3901 ctcggggcct taaacccaga attaactctc cttctttttc tccaaaatta aattccatct
     3961 tatttctgta ttcccacggg gaaggagaag ggataaaacc ctcataatct acttcttccc
     4021 aggaaatacc tcctaccttt tccagagttt gtcgagcatt tctttctttc gctttaacct
     4081 gctcttcata atccataaac tgaaaacggc aacctccaca cccttctaaa aagtgggggc
     4141 atattggctc tactcgagag ggagaaggtt ctaaaatatc tatcacctct cccagagcat
     4201 aatttttctt caatttagta attctcaccc gacacacttc gtcaattaaa acccccggga
     4261 cgaataccac ccggcccccc tcttttaaaa ccccctgccc ttcaggaaga gcaaaatcaa
     4321 ccactttccc ttctataatc tgtcctttat ccattaatta gtgctcctct gtctcataac
     4381 ttcataaatt ccaatcccta aagccactgc cacattgaga gaatttatta cccctcttga
     4441 tggtatcctc aaaaccatat cgcatgaaga acgcaccact cttctaattc cttcatgctc
     4501 tccccccacc acgagaccca gaggcatacc ccggaggtct gcatcccaga tgtctttatc
     4561 tccatctgct tccagagcca ctatccagaa acctgctttt tgaaactctt ttatcacctg
     4621 ggtaaaatta ttaatttgag ctatatctat gctctctatt cctcctgctg atgctctcac
     4681 aactggtgag tctaagggag cggagcgggc tttgctgatt aaaattcctt ccactccaaa
     4741 aaaagcagct gttctcgcaa ttgttcccag attagcaggg tcttccactc gatctacaaa
     4801 aaccactgtc gcttccggag aggttttatt tttctttaaa atttcctgcc aggtgagtga
     4861 tgcccgaggt tgagccagta gtaccactcc ctggtggtta acgtttttcc ccactaattc
     4921 ttctaaaaaa gttttatctt ttatttccca gggaattttc cttttctggg atagctgttt
     4981 aatatcttct attattttcc cctgtgctcc ttccatcaca aacaatctct tcactttcct
     5041 tttctctgac tttagagctt ctcttaccgg ctggcgccca taaataagtt cctctttcat
     5101 tgttcctcca tcttaaaaaa acgaggaccc aggggagtat cttctacaac atatcctcta
     5161 ctttttattt cttctcgtaa ctggtctgct ctctcccagt ctttatctct tcttgctctt
     5221 tctctctctt ctaccagctt tttaattgcc tcatcactta tttccatttc ttttcccgga
     5281 agaattccta atatttcttc taccattttc agaaaatctt taattttttc caatacttgc
     5341 acatcccact ttccccgacg aggagaaaga tactggttga aaaagcggag gaattctccc
     5401 aatactccca gagcttgagg agtgttaaaa tcgtcagcca aagcctcttg aaagctccgg
     5461 gagaattcat ctattttatg atattcttct ttatcttcct tcaaaagagc agaattatcc
     5521 tttatttttt cctctatgaa acctatattg cggagaaagt ttttaatccg ctcttcaaat
     5581 tgacgagagg attgtaaaag gttctcatcc agattgatag gactgcgata atgagcggaa
     5641 aaaattgcta acctcaaaac cgagggttca tatttttgag taagctcccg cactgttaca
     5701 atgttgccca gagatttaga catcttttcc tggtcgatgt taacataacc attgtgaagg
     5761 aaaaatcgag caaatggttt accagtagct gcctcacttt gagcaatttc attttcatga
     5821 tgaggaaata ttaaatctat tcccccggca tgaatgtcca gagtgggtcc taaatacttc
     5881 atagacatag ctgaacactc aatatgccat cccggtcttc cctcccccca aggactttcc
     5941 caagaaggct caccaggctt ggcttttttc cacagtacaa aatcagcagg attttttttc
     6001 tcgtatctag cctccactcg agccccttcc actaactcat caaggttctt gcgagaaagt
     6061 ttcccatagg aaggaaaacg agaaacatca aagagaacgt cgccttcttt ctcataagta
     6121 taacctttct cttctaaaat cttaatgaga gcaattatat cttcaatatg ttctgtagct
     6181 cgaggatgaa aggtggcttt tcgtatcttc aggttttccg catcgtgcca gtattcagca
     6241 atgtaacgtt cggctaactc ctgggggctt tcacctaatt cctctgctct ttttatgatt
     6301 ttatcgtcaa tatcggtaaa attctgcacc attataactt cccagccccg gtgttctaaa
     6361 aaacgacgca gagagtcaaa aaccacaaaa acgcgcgcat tcccaatgtg gattaaatca
     6421 taaacagtag gaccacaaac atagaagcga actttatccc cttcctgagg gaaaaaagtt
     6481 tcttttttct gggtaagagt attaaacagt tgagtttcca ttatctccca cttttgcttt
     6541 aattttttct aaaacctttt ctcttcctaa agccacaagc aagcggtcta actctggtcc
     6601 ttcttcagcc ccgattaacg cccagcgcaa aaccggataa aaaatagagg gaggaaaacc
     6661 gctctctttt tgccagcgac gcagattctt cttcacttct tcttcctccc agggaacagg
     6721 aaaatcattc agggaggaga gcaggaattt taatacttga agtttttctt catcttctac
     6781 tttttcttct tttactgaga tatagtctct caaacgctcc gctatttcct tcagagtaac
     6841 cgattcttct ctcactaaat ccagcaatag ataaaatctc tcttctccca gctctttttt
     6901 aaactccctt atctcttctc ctccccaggg ggtgagtttt tcttccagtt ctctaatatc
     6961 cattcggcga atataggttt tattccacca gttcaagtgg gagaggtcaa aaatagcccc
     7021 tcccttacct attcgctcta aagaaaagag agtgtagagc tcttctccct ctactatttc
     7081 cctcccatca gggaaagaat gtcctaaagt aagaagatga ttcaaaatag cttcaggtaa
     7141 aaacccttct tctctaaaaa atcgcacgct cacttctcct cttcttttgc tcaacttagt
     7201 tctttcctcg tccagaataa tgggcacatg ggcaaaaaga ggaggagtaa gctctaaagc
     7261 ctgataaagt aaaatttgat agggagtgtt agataaatgt tcttctcctc gtaatacatg
     7321 tgaaatatcc atcaaagcat catctataac acaagcgaaa ttataagtgg gaacaccatc
     7381 tgactttaaa acgatgaagt ctcccaagga attagtgtga aaaaccactt tccctcttac
     7441 taaatcctcc agtactattt ccttacctct cggaacccgg aagcgccagg atggttttat
     7501 tcccctctct tctttttcct ttctttcctc gggagaaaga aaaaaacagc gaccagaata
     7561 gcgaggagct ttgcccatct tcagcgcttt tttcctttct tcttccaact cttccacagt
     7621 acaataacag ggataaataa gattctcagc cttcaatcgg gcaatgaaag attgataaat
     7681 ttctcctctc tctgactgcc tgtaaggagc ataaggtcct cctcggtccg ggccttcatc
     7741 ccattctata cccaaccacc ggaggtcttc caggattgct tcttcaaatt ctttttttga
     7801 cctttcccgg tcggtatctt caatacgtaa aataaaatcc cctgcttctt tacgagctaa
     7861 agcccagtta aaagcggctg ttcttgctcc tcctaggtgc aaaaatccag tgggactggg
     7921 agcaaaacga gttctcaccc tccccattcc aacacctcaa ctttaacctt gctcagacca
     7981 tcttcatgaa tgtccagaac tttagcagca gcgagagata aatccacaac tcggtcttct
     8041 ctccagggac cacggtcatt cactctcacc actacttctc gcccatcttc cagattagtc
     8101 accaaaagaa cacttcctat ggggaaagtg cgatgagcag cagtaaaagc ataaggagtg
     8161 taaatctccc cgctggcggt ggccctccca ctaaacccat tactacacca ggaagctatt
     8221 ccttctgcta tttctttaac ctctttttct tcttttactc gataaaattc tatctggaca
     8281 ttgttcagcc aggagagagc caagctcaga gggtcagatt tattaaccaa agcatcttct
     8341 ctgagaactg taaaaagctt atgctgacca gcataagctg tagctttatc tttttcccat
     8401 accacatgaa aattcactat cttctgagaa tttttaaaca atttggcaag ggattgagca
     8461 atcactttag ccctttcttc taaagtagaa tatcctcccc cgtagcggat gcggagaatg
     8521 gttcgtcctt ccatatttat agaacaatag tgagattgag actcaagaga aatcaactct
     8581 tctgctgcgc tttctgtcgc tggagcagtg atcaccaacc cacaaatcac cagagctaaa
     8641 attttgttaa ttctttcttc cgcaaaaggc ttacagcaaa acaaccgatt ccttccccct
     8701 ttcccagact acccaaacct tcattagtag tggcttttat acctatagct tcctcgctca
     8761 cccccatggt caaagagaga gtttttttca tctcccccac aaaaggagca atgagaggtt
     8821 gctcagcaat aagggtggtg tccacatttt ctatttccca ccctttttct cgtattttct
     8881 tacatacttc ctctaaaagc aaagtactcc ttaccccttg cgtagagggt gaagttggag
     8941 gaaaccaata cccgatatct tgaaggccag cagctcccag aagactatcc atcagagcat
     9001 ggcaaagcac atccccatcg gaatggccat gtaatcccag ctcgtagggt atatgcaccc
     9061 cagccaaaaa caattttctt ccttctttta agggatgaac atcatacccc agacctactc
     9121 tccaggagct catttcttcc gccacctcaa taattcttca gccaaaataa ggtcttgagg
     9181 agtagtaatt ttcagattat cttctgagcc taacaccaga cgcaccggct tacccattct
     9241 ctcgaccaat gctgaatcat cagtcccttg aaatccttct tctttagctt tctccagggc
     9301 ttcccagatc agggaaatcc gaaaagcttg aggagtttgg gctcgccaga ttttttgtcg
     9361 atttagtgtc tcatcgataa tttctccatc caggctgagt ttaatggtat ccgcacaagg
     9421 cagagcacaa caagcagctt caaacttaac cactgcttcc acacatctcg ctaatatttt
     9481 atcatccacc aaaggcctca ctccatcgtg aataagcaca aaatccgccc cccggtcttt
     9541 cagctcccgg actccacaat aaaccgaatc ctggcgggtc tccccacctg aaacacactt
     9601 tttaattttt cgataaccat acttctccca cacctctttc ttaaacatct cctcatggag
     9661 agaattaatt accactatta tttctcctat ccagggagac ctttcaaaaa catccaaagt
     9721 atgagctaaa actggttttc ccagaagagg taagtattgc ttgggaacca ccgagtttac
     9781 tctctctcct ttgccagctg ccactattat agcgaagaac aaaatcttat tctcctttcc
     9841 agcttacttc aagaacgacc gctacctttt aatttcgcaa atatcattct acctgctgga
     9901 gtctgcagaa cactggtaac tactgtatct atggtagtgt taatatattt ctttccttcc
     9961 tctactacca ccatagttcc atcatcgaga taacccaccc cttgattttc ttccttaccc
    10021 tctcgaatga cctgcactcg caattcttct ccaggaagaa catagggctt tagagcatta
    10081 gaaagatcgt taatattcag tacttctacc ccttctagct cagcaatttt attcaagtta
    10141 aaatcattgg taattacttt tgcttccaat actttagcca gttttatcag tttggcatcc
    10201 acatccctga tttcaggaaa atctcggtct ataattttaa catttacttt tttctctttc
    10261 ctcatccggt tgagaatatc cagacccctc cttccccgtt gcctttttaa ggggtcttga
    10321 gaatcagcca cttgttgaag ttctcgtaaa acaaagcgag gaattaaaag gtctccctcc
    10381 acaaagccag ttttgcatat atcggcaatt ctcccgtcta caatagcact ggtatctaaa
    10441 atcttagctg aattaccccc tccccggggc ggcttcagtc ggggaatgcc agtaaaaata
    10501 gtaacaatat cttcttttct ccacataaac accgcaaaac ccagaacccc gcaaattaaa
    10561 gtgacagcga ggggaagaga aggattcttg aaaggtaaaa aagaaatggg ataggtgaga
    10621 agcactccaa ttactaaacc gactaccaat ccccctaaag ccaccaaaac atcaatagcc
    10681 gaaagatgat taaaaaattg agccagactt tcaaatagac cgagaaaaga gttttccttt
    10741 tttctgctaa gaaaataaaa taaaattaaa acaaaaacta ttaaagctaa tattagccat
    10801 ccacttttag acataaaatt tcctccttgc ctactagata attgccctac aactaaaaga
    10861 ttatatcatt ttatataaaa aaaattccgg ccgaggcaga tatccagcct ttttatcgaa
    10921 aaatatggta atttatctct aaaacaacaa gaaaagaact aaaactttat tataacctct
    10981 gaaaagataa aagacaaaat atcgcgagga gaaaaagcat ttcctcgtta agggagaggt
    11041 aaaccccacc tttaagaaag aataacctac aacgtataat aaaacttacg ctggaggaag
    11101 ccaggtgaag ctccctccag cgttgacatt ccaaatcttt ttaatctctt atgctttttt
    11161 ctcaacattg tccggccgcc aaggtaaggc tcacaacgac cgttataaat aggttaacaa
    11221 ggcacagata agagaaggaa tccctgagta gatgtgaatt agacagggaa aaagcaaaac
    11281 tgccacacat atctggtctt cgttttttag tttacactgt cagagtccct gctgtctggc
    11341 aatgatatgt ggtcataaga ggcgaataaa attaatgtag tggtagaatt actcaatttt
    11401 gaccagaggt ctctaacgcc acactgcaaa aagagaagaa ataattacag tgaatcttac
    11461 ggttgattaa tcggtttccc tctgctgtct tcgggagaaa aacagagatt ccccaacgca
    11521 atgcgtgtgt ctgagttagc ctatgagacc ttctttttcc agcgcttctc taacgttaga
    11581 ataccctacc agcgcgattt ctccccagcg ctctgaaaga aaatcacggg aatgataaga
    11641 agagactacc actcttttaa ttcccatccg ggcagactca ttgattcgct gctccaaaag
    11701 aggaacagga cgaatctctc cgctcaaccc cacttctcct aaataaaccg tttctttgtc
    11761 tatttctcgg ttcagacgag aagaaaccag actgaagcat accggaaggt ctaaagcagt
    11821 ttctgaggtt ttgattcccc ccaccatact gagataaaca tcctgtcttg agaagcgcag
    11881 acgagtgttt ttttcaataa tcgccaaaag cagatgaagg cgatttaaat ccatacctaa
    11941 acttacccga cggggaaaat caaaatagct ggggtgaacc agggactgga tttccaccac
    12001 catcgctctt tttccttctg cccagacggt gcgggctgtg cctatcccgg ctaatttact
    12061 ctcctccata aaataatgag aggggtcctt tacctctacc agccctctct cttgcatctc
    12121 cagaattccc acttcttgag tagaaccaaa acggtttttc atgctccgca aaagacgcag
    12181 aggatgattg gcatctcctt ccagataaag cactacatcc actaaatgtt ccagagcttt
    12241 aggcccggcg atggcgcctt ctttagtcac atgtcctacc aaaaaaagaa caatattttc
    12301 ttttttggct atttccataa agcgagagct taaatcccgg agggtggcca cgctaccggg
    12361 caaaaattcc aaattctctc gtcccaagtt ctggatagaa tcaacaatta ccgcttgagg
    12421 ttgaagagaa gaaatccctt ctcttatctc ctcaaaagaa ttagtagaaa gaagaaaaag
    12481 ggggggattg caatccagac ctaaccgttc tgctcgtaga taaacctgat aaagagactc
    12541 ctcactacta gcataaagga cttttttccc ctcccgggca ataccctcag caatcttcaa
    12601 taataaagta gatttaccga tgcccgggtc tccactcagg agaattaaag acccttctac
    12661 taaacctcct cctaaaaccc ggtctacttc tttcaagctg gtagaaaagc gatcacccaa
    12721 ctggaatttt ttttctttaa cttccgctaa agaaagaggg ggagaataag aagagcttct
    12781 ttctttttta gtttcttctc gggaaacagg caaaaaagaa ttccactccc cgcattgagg
    12841 gcacttacct aaccagcgag gagtggaata accacagtta ctgcaacagt aattcatatt
    12901 acccttttag tttttcaaag aaaactccag aggaagtagt ttggtaaaat gtatctctcc
    12961 ctcttctact cccactaaaa ttcgatctcc ttccttaaag acacctttga gtataaggtc
    13021 agaaagagga tcttctatca atttttgaat agcccggcgt aaaggtcgag caccaaagtt
    13081 agggtcatat ccttcctgag caataagaga gcgagcctca ggagtcaggt ctatctccat
    13141 tttgtgttcc ctcatacgtt cttgagtttc tttcatcata aggtccacta tagcttcgat
    13201 ttctttctga tttagaggac gaaatacgat gatctcatcc acccggttta ggaactcggg
    13261 agtaaagact ttttttacct cttcagtaac tttttgctta atgtcctgat aagacataac
    13321 ttctttttcc gacctctcac caaagccaat gttggactgg gaagaaataa gacgggctcc
    13381 caggttagaa gtcataatga taattgtatt tttaaagtct accactctac cttgagcgtc
    13441 ggtcagacgg ccctcttcca taacctgcaa caatatgttg aaaacatcag gatgggcttt
    13501 ctctatctca tcaaagagaa ctaccgaata tggttttctt ctaacttttt cagttaattg
    13561 tcccccctct tcataaccta catatcctgg aggagagcca attaaacgag aaacagtgaa
    13621 tttttccata tactctgaca tgtccaagct aataagatta tcttccttgc caaagagaaa
    13681 ctctgcgaga accttagcca gctcggtctt gcccactccc gaaggaccaa gaaatataaa
    13741 agaactaata ggacgattgg ggtctttgag cccggcccgg gaacgccgaa tagcccggca
    13801 tactgcctcc accgcttctt cctgccctac tatacgttca tggatttcct tttccatgtt
    13861 taccaatttt tctttttctt cggtatctaa ccgagaaatt ggaataccag tccagataga
    13921 aaccactgtg gcaatatctt cttctcccac cagaggtcga gaactatcca cttccttcaa
    13981 ccagttttct tttctatctc gatatttctt tttcagcttc tcttcttcat ctcgataatg
    14041 cgctgcctct tcaaaatcct ggtttttaac cgctttttcc tttagttttt ttatctcctc
    14101 tgcttccttt tccaactctt ttaaactttc tggcaaaatt gtagcctgca gtttaacccg
    14161 gctggaagct tcgtccatga catcaatagc tttgtcagga agataacggt cagcaatata
    14221 acgctgggaa agtttaaccg ctgcttctac tgcctgatcc gatattttta cccgatggtg
    14281 ttcctcatag cgctctttaa gacctcgcaa tatttctacg cttacctccg ccgtgggttc
    14341 atccacataa acgggttgaa accttctctc taaagcggaa tctttctcta tatacttacg
    14401 atattccatg gtggtggtag cacctatggt ctgcaattca cctcgagcca gggctggttt
    14461 taagatattg gcagcgtcaa ttgctccttc tgctgcccct gctcccacca gagtatgaat
    14521 ttcatcaata aaaaggatta cttccttact ctgcacgatt tctgaaacaa tcttttttaa
    14581 tcgcttctca aactctcctc gatatttagt accagccact atggcagcca aatccaggct
    14641 taccactctt ttacccttca aaatttccgg aatatctccc tttactattt tttgcgccag
    14701 accttcaaca attgcagttt tacctacccc tggttctcca agaagaaccg ggttgttttt
    14761 ggtacgacgg cttaaaatct gcaccacccg ctcgatttcc ttttcccggc caattaccgg
    14821 atctaactta ttttcaatag ccaagcgggt taaatcccgg ctgaattcat caagcaccgg
    14881 agtgcgactt tcttgagaag gcttactggt ttcggacaaa cctcccactt catcaggatt
    14941 ctcacttaaa atttcataaa gcttagcccg aaccatttct aagtctaatc ccaggcgctg
    15001 caaaatctgt gctcctatcc cttctccctc gcggagaatg cccagaagaa tatgttctgt
    15061 acctacataa ttatggccta aacgacgagt ctcatccaga gccaattcca gaactctctt
    15121 ggctcgagaa gtaaaagcta cttcccggga accctggtac tctctctgat ccacaaagtt
    15181 ctccaattct gtccgaacta aatccatagt tatatcaaag ctatctaata tcttagctgc
    15241 gatgccttct cgctctcgta acaaacccag aagaaggtgt tctgtcccaa tatgattgtg
    15301 cttgagacgt acagcctcgt cttgagctaa gataatcact tttctcgctc tctctgtata
    15361 tctctcaaac atcgttccat atcctcccta attctattcc taaaaagcct agtactttca
    15421 gactctgccc tcttagtaac cccccgttta ttattttatc ccataaatca gcactcttca
    15481 taaactcaaa accccaaccc tcaggcaata ttccctcgtc cctggccaaa aaccaaaaag
    15541 aaaggaaatc taaaatcttt ttctcatttt gttcttcatt cccctccatt aactcctcat
    15601 atagacgttc catcatctgc caaaaaacat tctgacgaga ggaagatttt actaaagacg
    15661 tcctaacttg tttttcccat tcccagatac ctttcccaaa atcttgcatc atcttcaaga
    15721 tttctgtttc cttcactccc aaagtaaaaa ggttagaaaa tacaaataga tgagctagcg
    15781 cctggtcgtc ttcaccatag acctctacca gaaatctgtc attatcttta aaccattgga
    15841 aaactctctt ttccccgtat aagaaggaaa gagcaggcaa atgtagcctc atataaattt
    15901 taagacctgt ccccaaacgc gaaggagaag aggtgaggta tccccactgt tcactgtagg
    15961 cgtactccag cttactctcc caaaaatcat cccatttatt taatctcttc cagtaagatt
    16021 ctaaattcct gtattcacct tctgcaaaca aagacagatg gtcctgatgg ttgataatag
    16081 ctcccaaatt acctttatga atcaaaattt tttccggaga atcggatagc agttgaggaa
    16141 aaacccagct caatggctcg acttcccggg aatgagaacc tatttccacc caggaaaacc
    16201 cctttcgagc taaagaagaa taggccttct ccactctttc tttcacttct tcttcccaca
    16261 gaggagaaaa gtaaatagaa gaaggaaagg ggatacgggc gagatttcga gataaaacca
    16321 gagctactct attaaaacca tcaaggagtc tgctttttcg gctcgaaaac aaccgcttac
    16381 cgagagtaaa actcatttta gctgagaacg aagcttctcc atcacctttt ttaaatgtcc
    16441 tgctttctca taattttctt gctctatcgc attttccaaa tctcgcccca ggcgaaaaat
    16501 agcattttgt atttttttct gtctttgcca gttgatgggt attttgcctc tatgatgcat
    16561 ccccagctga ttcttagata gccagctttg catctcttta gagtaggaac tgtaacaatg
    16621 agaacagcct actgctcctc cttttaagaa atcttcccac tcccactggc aaaaagagca
    16681 ccgcttgcct ccctctttct ttagaggaaa aaatctggtt gacggtgggc tcttatatga
    16741 accaggggca actacccgag aaatagagac ctcctgagaa caagcagggc aaagaaaaag
    16801 cttattctta agttctcctc ctcctataag gtagactact actttagcct ccctccagcc
    16861 acagatatca cacttcattt attccaccca tctcaaaact tgaagaagga tttctctaaa
    16921 aattcgagac cgtaatcgat ttctatctgc taccgaggaa aaatctaagc cagctacctt
    16981 atttaaagca tctatggtag ctgctcggac gagagcaccc tcgcgaggag tgataaactc
    17041 ttccctttcc agccagctta ctaaatcttc tacatcgctt tctctcaaag actcctcgtt
    17101 atcatctaag taaacaacta caggttctaa gcggtcaata ggcactctcc tgatcctcac
    17161 ataaccgcta cctccccgtc ggctctctac tataaatccc cggaatacat taaaacgagt
    17221 ggctaaaaca tagttaatct gagaaggagc gcaatcaaac tcattagcta accttcccct
    17281 ctggatggta atcatttggt caggggaacc gtctattagt ttgattatat gttgttcgat
    17341 gtcatcgctt agagagtaca ttctccttcc ctcccttgac tattattgac tttcccctac
    17401 attatataaa gaaaaccccc tatttgtcaa tccgatgagg ggcgcaggag gggaaaaaga
    17461 ataacctctc gaatagaaga agaattggta atcaacatca ttaatcggtc aattccgata
    17521 cccaaacctc ccgtaggtgg cataccatat tccagagctt caatgtaatc ttcatcaatt
    17581 aaatgagcct ccaaatcccc ctcatctctt ttccggagct ggtctgcaaa tctctttttc
    17641 tgctcaaaag ggtcgttaag ctcactatag gcatttccta tttcttcttt accaatgaaa
    17701 agctcaaaac gttccgccaa ttgaggattg tcttttttag ctttagctag aggagaaatc
    17761 tccaccgggt aatcaaaaac aaaggtgggg tttactaaac gtggctggac aaatttctct
    17821 aaaaattcgt taacaaattt ccctcgatcc cagttatctt cataagccac tccccgctct
    17881 ttagctttag ctttaatggt ttccagagag tcttctacaa tgtctacccc caccaattcc
    17941 tggatgactt gcggcaaact tattctgtgc cagggaggag tgaaatctaa aaccatagac
    18001 tgatactcta tttcatagct accagtgatt ttttttacca cgtgcgataa catttcttct
    18061 gttaattcca tcatcacttg atagtcagca taagcctggt aaagctccag catagtaaac
    18121 tccgggttat gtcgggtgga tagaccctca tttcggaagc tgcgatttat ttcataaact
    18181 ttctctaatc ctcctacaat taagcgtttg agaaaaagct ccggggcaat gcggagataa
    18241 agctccattc ctaaagcatt atggaatgtt ttgaatggac gggccagagc tcctcccggg
    18301 atcacctgca tcatgggagt ctccacctcc cagaaacccc tttgatctaa aaaggctcgc
    18361 aattctttta gcagttgaaa gcgaaggtaa aaaattctcc tcacattggg attcataatc
    18421 aggtctaaat aacgcttacg atagcgaact tccacttctt tgagtccatg ccacttttca
    18481 ggaagaggac gcaggcattt actaagaaga gtgaaattct ctaccagaac tgtcaattcc
    18541 ccggcatgag tgcgaaaaac tttcccctcc actcctatta tatcgcctac ttctatttct
    18601 cgaagaagag taaagctatc ctctcctata atatcttttc ggaaataaat ctgtattctt
    18661 gccgtccgat cttgcaaatc agcaaatacg gctttgccat gcctcctcaa agccattatt
    18721 cttcctgcta tctttacctt tatatcttta tccaccagat tatcggcatg gttttttatt
    18781 tcttcaatat catgagtttt acaaaaacta ccattgtagg ggtctatacc taaatctttt
    18841 agagcctgga ttttctctct tttatattca tactcagtat tttcttcata tcttgttact
    18901 tcctcgtcca ttagttttta ctcatccttc ccttttacct ctattatacg ataacgtacc
    18961 accccggcag gaacttttat ttccacttct tcgccttctt ttttattgag gagagcctgc
    19021 gctaaggggg attcaaagga aattttccga gctgaaggat cagcttctac cgagtcaaca
    19081 ataagatatt ctatcttttt attctggtct aaattctcca aaattacccg cgaacccaga
    19141 gtaaccctgt cggtgttacc tccctcagta tctactatct ctgcttttcg taacttctct
    19201 tcaatagata aaatcttccc ctcaataaaa gcctgctcat tctttgcctc ttcatattca
    19261 gcgttttcac tgagatctcc aaaagaaaga gcatgactaa tcttgtctgc tatttctctc
    19321 ctcttcactg tttttaaata ctttaattct tcctctaact ttttatatcc ttctctagta
    19381 aggacaatct ttccgtcatc ttttttcttt tccatcacta ctacgcctcc aaccaagaac
    19441 tttgtcagat gagaattata gcataaaata taggaattac tacaaattgt caagactctg
    19501 ggaggctgct tgaaaaatac taccttttcc agagcataat tcctttctat gatagaagct
    19561 ttataggtct cagctacccc cttataaacc cggctataga aggagttccc ttataacccc
    19621 tttaaggaat taaaataaaa atgtaaaaac ttaaaaacat aaaaacccac tgagaaagac
    19681 gcttcttctt tctcaatata ggaggaaact gatcccctat gatccccttt atccctcatt
    19741 cctgaatgcc tcttttgccc cttattcgtc acccctgagt gtttctacga ggggtccaca
    19801 gtcttttaaa ccaaagaaaa aagcaaaaac gtggattccc gataaaagat ttcgggaatg
    19861 acggcggggg tggattccct gaatgcctct attacccctt gtttgtcacc cctgaatggt
    19921 tctacgaggg gtctaaattg tttaagggca aaaaacttgg attcccacct cctcgtcacc
    19981 ccggcaattt ttaagccggg gtccagaact ataaagggga gaccccttta taaaccccca
    20041 aaaagacagc agcctacact aaaaaaaaca aaaaatgcaa aagactactg aggatga
//
LOCUS       DAOPSS010000014        19949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2298_length_19949_cov_8.699005, whole genome shotgun sequence.
ACCESSION   DAOPSS010000014 DAOPSS010000000
VERSION     DAOPSS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 19949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19949
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_19949_cov_8.699005"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..987)
                     /locus_tag="PKN31_01745"
     CDS             complement(130..987)
                     /locus_tag="PKN31_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA98686.1"
                     /translation="MRIIKPFDPWQGKWCSCPAKYTLNPYTGCAHRCLYCYTTSFIPR
                     AFEVREKPGILKKVEKDLEEIDSSRYLSLSNSSDPYPPEEEKRGLTRAILNLCREKGL
                     PVLILTKSTLVTRDIDILKTMKAAVSITVTTFNSSIASVLEGGAPSPQKRAQALIELH
                     EAGIPTILRIDPLIAGINDKPEEWKEILSLLSPYVNQVVVSTFKPRPDSWKRITTTFP
                     SLQKTKDWYTKKIGNSIYLEDNRRVKLLEEARATIHSFSLPFSSCREGLANWNDLPCD
                     GSDLLEINR"
     gene            1044..1562
                     /locus_tag="PKN31_01750"
     CDS             1044..1562
                     /locus_tag="PKN31_01750"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOA98687.1"
                     /translation="MSFRKSDWLEAGKIALETLENLGGVFLISNDKESLRPNVMTIGW
                     LELGIVWRRPIITVLVRPSRHTFSLLEENPFFVVGVPPSSWKKEIDFCGSTSGREVDK
                     FSQCGFNPVNINDFPVPVIEGCEVAFGCEIIQKTRVEAGNFIASILDEFYPQDDYHSI
                     YFGEIKESWSRG"
     assembly_gap    1709..1808
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1809..2152)
                     /locus_tag="PKN31_01755"
     CDS             complement(<1809..2152)
                     /locus_tag="PKN31_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOA98688.1"
                     /translation="MSLVIWVIVVIAVIFLASAIRVVREYERGVIFRLGRLVGAKGPG
                     LFFVVPFIDQLIKIDLRVVTMDVPKQSLITRDNVPVTVDAVVYFRVLSPEDAITKVEN
                     YIAATSLISQTTL"
     gene            complement(2155..3441)
                     /locus_tag="PKN31_01760"
     CDS             complement(2155..3441)
                     /locus_tag="PKN31_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOA98689.1"
                     /translation="MRDYILPYFYDTIKTMKRETKFSFLLLLLIIISFLWVPLVQAQE
                     KVILGKVEGIINPVIEQFVVRLIEEGKKEEAKVILILLDTPGGLEESMRNMIKAILQS
                     PIPVTTYVYPPGSRAASAGSFIVMASHHAFMSPNTTIGAAHPVTVEGQEVSEKIVNDT
                     ASFIRTLAETRGRNPEIAEKMVRESISLTEKEAQEKGMIDGIAESPEEIIEFLGIKNP
                     QIIEVKMKEKEKFLHFLLNPTLAYLFLVIGALGIIFELSNPGAIIPGVFGTILLLLAF
                     YAFSIFPINLAGILFIFLGLLLFILDLLVTPGIGVLTAGGAVALFFGSLMLFDIQGGV
                     TLPLNVIITTVLCVAAFFVFALGMALRTMRKKVVTGIESLIGEVGTAKEDLNPEGFIT
                     VEGELWWARAESPVKKGEKVIVSQKEGQTLIVKKEE"
     gene            3468..4802
                     /locus_tag="PKN31_01765"
     CDS             3468..4802
                     /locus_tag="PKN31_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA98690.1"
                     /translation="MNILLVAPGTDNLQRKVRVVPFPQASLPLLAALTPSHHEVKIID
                     ERLENIDFDGKWDLVGITVMTATAYRAYYIADEFRKRGTKVALGGIHPTALPDEAIQH
                     ADSVVIGEAEGLWEELLGDLEEGKLKKFYRHDCPPPLAGLPCSRLDLLDKRYLLRHVF
                     QTTRGCPHNCGFCSVSTFMGRRYRHRPVEEVIEEIGHYPNARILGFLDDNIVGNPHYS
                     RELFQALIPLRKKWASQGTLKLAENEELLKLAAKSGCIALFVGLESVNEDNLKKMHKN
                     FNQVDKYKKLIDRFHEYGIMVIGSFVFGFDEDDYSIFDRTLNFLESANLDFAQFSILT
                     PLPGTEVFKQFTAEERIFSYDWRKYDFAHVVYHPLRMTPQELQDGYNYISRQFYSLPR
                     IGKRTIRNWRYAHYFLPASIYYHWVCRHPKSLPEEAASAPLSLKDKKQSPVV"
     gene            4836..6008
                     /locus_tag="PKN31_01770"
     CDS             4836..6008
                     /locus_tag="PKN31_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOA98691.1"
                     /translation="MSLPASELEIRLQKVRELLTLKNLSCGLIYYDELNIANGWYLSG
                     WCPQFESGAVLVPVQGEPMILGGPESEPFAQIDSAIKKTRNIPVFMVPEEEYPNAYIS
                     SFAEVFKEIGMSEADRRVGIVGLEKMPLGVYQRLLAELEGVELVDLTPEYENFRKLKS
                     PWEIEQIKQAFAIADESIKIMEELVREGVFETEIAGAGEGRARSLGANWFAFKTIVAS
                     GIRSNGVVPTASSEKSLQNGETLMVGISPRFNGYAGVAGYTYVVGNKINEAQRTCFND
                     MVEAYRITKSNLVPGAKGRDIDSKTREFLVKKGYAKYLVCPFVHTIGLMEAEAPFFGP
                     NSNDTLQPGMTVCIDVSVFSVPEVNGIRLESGYLITENGAVPLSPFMEQKILNSRI"
     gene            complement(6208..7653)
                     /locus_tag="PKN31_01775"
     CDS             complement(6208..7653)
                     /locus_tag="PKN31_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA98692.1"
                     /translation="MEEELTKSHHANRYRILTAVMLGGIMGPIDASVINVNYHIIGNH
                     FGVALTEAQWVVMVYLLTVSSLLLFYGRLGDILGYKKIYLIGLSGFTITSLLCSLSYF
                     LPSISWLILFRAIQGTTAGMMMSVPYAIIVSSFPPQERGRALGINAISVSTGLAIGPS
                     LGGFITHLAGWPYIFLINVPIGIIGLWWGNKVIPELKGKPGKIDVGGAITNCLFLFSF
                     LFFLNRFPAQGINSTTLFTAVVALVAFFSFLIIEKRADSPLINLQLFHNLSFSLANLC
                     ALLNFMSQYILVTLTPSYLNRVLQYEQSEVGLLLTIFPLTTLVVAPFAGSLSDRINPR
                     FLAFLGATFCTLSLFSLSQLPIYVGTADIAWRLALFGLGTGIFQSPNMNIAMGSVPRE
                     HLGVASSILATVRNVGMVLGTATGGGIISASVPSSILQGLEKLLDKEASIFMQGLKNA
                     YLWGAILTGIAALVSLLIPHYNSGASYQSHN"
     gene            complement(7703..9112)
                     /locus_tag="PKN31_01780"
     CDS             complement(7703..9112)
                     /locus_tag="PKN31_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOA98693.1"
                     /translation="MEEKVVENEEDFLEGEDFNTILPEILDKKHIRSKAFSLSLPALL
                     NMFLISFVGMVDMIMVGRLGPAAISAIGMVNQPVLLIISVFMALTVGTTALVARFIGA
                     KDIDSAKDVARQSIVVSIFFGIVLLPLLYIFAPQIERAMGADPEVLVLGVAYMRMIAL
                     GTPFNVLAMALGAILRGSGDMKTPLIADIVANLSNILGNYILIFGKLGAPALGVAGAG
                     IATSIARFVASAILFYVLYREKTVLKLSLKESYRPNFSTLKRIFNIGIPSALEQFVLR
                     AGQLTFVRIVAELGTIAFATHQIAMNIQSLSFMPGQAFSMAATTLVGQLLGADKPDIA
                     EESARQTRLLGMIVSGISAFTIFFFGKYITMLYTNDSAIIEQSRICLRIIALIQPAQS
                     TQFILAGALRGAGDTRFPLYSTIIGIWGMRVAMAYLFVMTFGWGLTGAWLAIALDQIV
                     RAMVIYSRFKSGKWKYFRI"
     gene            9372..9551
                     /locus_tag="PKN31_01785"
     CDS             9372..9551
                     /locus_tag="PKN31_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98694.1"
                     /translation="MQRLEVYWRKISPGFMPRTDVSTHVGIESLKTIETTGKDVSLQC
                     CCGFCSEPLEMWRKG"
     gene            complement(9615..11279)
                     /locus_tag="PKN31_01790"
     CDS             complement(9615..11279)
                     /locus_tag="PKN31_01790"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOA98695.1"
                     /translation="MKILEGLALHFLTEEIEKEAKGARIQRVFTTPRRETWLEIYSPG
                     DREKSLVISLYPQENFIFTTSRKEVQKEKPNNWQQLLNKYLVGGKIISLTQVGWDRVL
                     KIEIKNPQLFEEGNLYLFVELTGRNTNAILTDQSKPPHILGVYRRVGKDQNRFRTLLP
                     GEKYSLPPQKNKIDPFFFLQEKEKDLLFSSSPEDLQRELVQQIDGIGPFLSQIIAEKV
                     GGGKSPREAMKETISPLRESNYQIRVFFSSSGEPQGIFWDSVTPYSSLSYQEFPSFNQ
                     AVECFHFSWWERQEEFIQQKIKEKRMKEELDFLEKEREKIESLIKNEEELEELKIKGE
                     LLKILPGLEVISKTEEGITVKNVFSSPPQDIFIPLNPRLSINQNMQNYFRLYRKGREK
                     NLKLRERLKELEEKERKVEKIFEEELLPEKEGNKAFLSSPEKALRFKTSQGNEIWVGK
                     NAKSNHFLVTKIASPQDYWLHVRDLPGSHVILKIKNKENEEEEIQKAARVAAYFSSVR
                     GESRAEVIVTQVKYVRTLPSQIGKVTYRREKTLLVKPQLPGDVTPW"
     gene            complement(11312..13546)
                     /gene="glgB"
                     /locus_tag="PKN31_01795"
     CDS             complement(11312..13546)
                     /gene="glgB"
                     /locus_tag="PKN31_01795"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOA98696.1"
                     /translation="MSNSPVTISKEKMESIINADCHDPFSILGMHPLEEEKGVCVRVF
                     IPAAKKIKVIDRYNSQNSWLMNKVEERGFFELTLPGKDFFAYFLEIEEEEGIRVTADP
                     YSFLPFFSEFDLHLFNEGTYHFAYERLGAHLIEVGGIKGVYFSVWAPNARRVSVVGDF
                     NRWDGRIHQMRVLGSSGIWEIFVPQAEEGAKYKFEIKTQEGNLLLKADPYAFFSELRP
                     ATASIVYNLSPFPWKDEEWMEKRKSQNFLSSPWSIYEVHLGSWKRGKDNSFLNYRDLA
                     HQLVAYVKEMGFTHIELLPIAEHPFDASWGYQVTGYFAPTSRFGTPEDFQYFVDYCHQ
                     EGIGVILDWVIAHFPKDAHGLGRFDGTALYEHLDPRRGEHPHWGSYIFNYGRNEVRTF
                     LISNAFYWFKEYHIDALRVDAVASMLYLDYGRNEGDWIPNPYGGKENLEAISFLHYLH
                     ENIYHYFPGIATIAEESTAWPGVTLPPYLGGLGFLFKWNMGWMNDFLTYISKDPVYRK
                     HHHQNLTFPLLYAFSENFILPISHDEVVHGKRNLIDKMPGDWWQKFANVRLSFATMFG
                     YPGKKLLFMGTEFGQWREWNHDEELDWFLLGYETHRQLQRLMKDLNHLYTTEKSLFEL
                     DYSWQGFEWIDCNDADNSVISFMRKSKNPGDFLVFVSNFTPVPRFNYRIGVPREGFYQ
                     EILNTDSEIYGGSNLGNWGGVWTESIPAHGRSFSLNLTLPPLGSLIFKWRGAPGILNR
                     EDKL"
     gene            complement(13543..14409)
                     /locus_tag="PKN31_01800"
     CDS             complement(13543..14409)
                     /locus_tag="PKN31_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOA98697.1"
                     /translation="MLLQTFCHIPGVGEKIERRIWEEGITTWEEALGYPALEKIVPSK
                     FSRSARSFLYRSLQKLEGGEARFFKEHLDTHDHWRLFPHFQNDTVFLDIETTGLNPPQ
                     DYVTAITIFDGKRIKTFIQGINLPEFKEEIMKYKVIVTFNGKCFDIPFLEQHLGIKFP
                     HVHLDLRFILRSLGFRGGLKACEKAIGIDRGDLRGVNGYTAVLLWQKYREGCPEALET
                     LLAYNVADTVNLEKLMVFAYNEKLRNYPFPQLSLSLPPKKIITPYQVHPEILKEINHE
                     YWRTVSQKEVKV"
     gene            complement(14421..15347)
                     /locus_tag="PKN31_01805"
     CDS             complement(14421..15347)
                     /locus_tag="PKN31_01805"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HOA98698.1"
                     /translation="MKEALTTILISFLAILTTPLYADMGAIVPTGGVKVEEVAQKAII
                     AHDGFEEIIILSTDLIASQNTPVLRLIPFPSLPEIIEVDNEIWKRLSHICEKHNLQYL
                     TFYKGGEKEEAIKVEYQVTQGAHKITVVKVNDSEEFLEWVRSFLLQNNLPSRFQEEKL
                     QNLVDDYLKRGINYFVLDIVDFQENRERVEPVGYIFLSRNFYYPVKISGLFENSGEVE
                     LFILSDRGEILESLIDFWPGWSGGGISSTTVLEEKDLEFLHPEIHHLLGKRGILCAFR
                     HPRFQELQEDIIFEMPVKEIKPQKSFHFTKKN"
     gene            complement(15905..17281)
                     /locus_tag="PKN31_01810"
     CDS             complement(15905..17281)
                     /locus_tag="PKN31_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98699.1"
                     /translation="MIPNNPREQLIKLGAEKLADALLDLSTRSDQAAQLVEQLLSPPR
                     ENLRRFKARIRGLRQRKRFLGAREAQSYASDLSDLLADIQRNIEDPKIGLELVVAFFE
                     SDNKVLESCDDSYGNVGEVFRYDATELFTHYARDIEDKSYLSDLVFKLYEEDEYGVRE
                     ELVDHAFQFLPEETLRSLAQRFWENAENIDKTVKDNQYDARHSLFAVESLARQLHDAP
                     LFERAALATWPDLSSKTCLDIAEVYLEAQESEKALDWIKKVPPEMALDDYKRDKLLLD
                     IYRKTNNQEKLAEVAWKIFRQHKGVDNLEELLSIIGEDQREKVIAETSQEIMANPSSF
                     YYDISFLLDTNQVDLAQKYVLENEDTLNGDQYGLMLTLAQRFEKENRFLVSTIIYREL
                     LESILHRAQSKYYQYGVRYLKKLEKLAPQVSDWQGVLPHELYFKKIAETHARKKSFWD
                     KYEREEQK"
     gene            17402..17830
                     /locus_tag="PKN31_01815"
     CDS             17402..17830
                     /locus_tag="PKN31_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98700.1"
                     /translation="MKNKLIVAGVIFTCLVLLVGCGDEKWNPLDVEIVDHETGCLALE
                     EESITTLWEGEDIVATTIFSTSVPCYQIRSISAREKEDKIEVKIKLEREAGACIECIG
                     FQKITFRIKNPRLYPEINLELQVEVDGQVLIQTIIEQPAT"
     gene            17963..18760
                     /locus_tag="PKN31_01820"
     CDS             17963..18760
                     /locus_tag="PKN31_01820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOA98701.1"
                     /translation="MEKLNQLLLNRVAIITGGTRGIGLGIARVLSQAGAKVALVYRSD
                     NQRANEAIEELKKEGCEAISIQGDVGIKKEAERVVSLVAEKWGKVDILVNNCGILDFT
                     FLENLTEEQFDQVLRANLKSALFCTQAVLPFMKQEKFGRIINASSISGHFADVGQIAY
                     GVSKAGVEMFTKIASAELAPYGITVNAYAPGIIETDMTRSMIEERGHLQKEQIPLNRF
                     GTPDEVGALVLFLSLEEAGYITGEIIGVDGGMLKVQNAWRARYEGKE"
     gene            18824..>19949
                     /locus_tag="PKN31_01825"
     CDS             18824..>19949
                     /locus_tag="PKN31_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA98702.1"
                     /translation="MKKQFLIVPFFLALIVIGLGLSGMGAVIPEMAQQFSVSYSVVGR
                     VFLFHGLGYFVSILLAGILGDIINQVSILRLGIFLSLAGFGGISFLPSFPLVMASFLL
                     MGVGLGFIDAMISPVANSVFTEKPATTLNIIHAFFGLGSLAAPRLYSFLVVEGYGWRN
                     LYQICTLFTLITLVIFLFPFIPRKNQPSQFKELLGVFKQPALWIMGFTTLFYAGGVST
                     LNGWLVSYLEGEGIVRGEGAIFLSYFWLGIMLGRLILSPVSDKVGHLNTIRLNALGGI
                     VFVSLFLLLPFHSTSAVLLLFGTGFMLSTLIPTTVSYALATFPEVTSTASSWVLFNNS
                     LGSFLFPWLGGVIGTAFSLRLTMIFVPLFLILMFLFQQWLA"
BASE COUNT         5764 a         3819 c         4115 g         6151 t
ORIGIN      
        1 acactctgag caaacctcga gatatagatt taatgaagga gttagccata tccattctct
       61 gtagtggtga tgaaacagcg gaggattttt atccctcgag tgatatttct cgctttaagg
      121 aaatggactt tatcgattaa tttctaataa atcacttcca tcgcaaggca aatcgttcca
      181 gttagctaac ccttcccgac acgaagaaaa aggaagggag aaagaatgaa tggtggctct
      241 cgcttcttcc agcaatttta ctcttcggtt gtcttccaga taaatactgt ttcctatttt
      301 tttggtatac caatccttgg ttttttgtaa ggagggaaaa gtggtggtaa tccttttcca
      361 ggaatcgggc cgtggcttaa aagtagagac taccacctgg tttacatagg gagagagaag
      421 agatagtatt tctttccatt cttcaggttt atcattgatt ccggcaataa gagggtcaat
      481 cctcaatata gtgggaatac cggcctcgtg gagttcaatt aaagcctgag cccgcttttg
      541 aggagatggg gctcctcctt ctaaaacaga agcaatagag gagttaaaag tggtgacagt
      601 aatacttact gctgctttca tggttttcag gatatctata tcccgggtaa ccaatgtgga
      661 tttagtgaga ataagaacgg ggagaccttt ctcccggcat aggttgagaa tcgctcgggt
      721 gagtcctcgc ttttcttcct ccggaggata ggggtcagaa gaattagaaa gagagagata
      781 tcgggaagaa tctatttctt ccaggtcttt ttctactttt tttaagatac caggtttttc
      841 tcgcacctcg aaagcccggg ggataaaaga agtagtataa caataaaggc agcgatgggc
      901 acaaccggtg taagggttga gagtatattt ggcaggacaa ctgcaccact tcccctgcca
      961 aggatcgaaa ggtttaatta ttcgcattga ccttctcctg tttctgtttt attataatat
     1021 aaattatcat gggaggtgga attttgtctt ttcggaaaag tgactggtta gaagctggaa
     1081 aaatagctct agaaacttta gaaaatctgg gaggagtttt ccttatttct aacgataagg
     1141 agagcctccg tcctaatgtg atgaccattg gttggttaga attagggata gtatggagaa
     1201 ggccaataat taccgtttta gttcgacctt ctcgccatac cttctctctt ttagaggaaa
     1261 accctttttt tgtggtgggt gttcctccct cttcctggaa aaaggaaatt gacttctgtg
     1321 gtagcacctc aggaagagag gtggataaat tttcccagtg tgggtttaac cccgtcaata
     1381 taaatgattt tcctgttcca gttattgaag gatgcgaggt agcctttggc tgcgaaataa
     1441 tacagaaaac cagggtagaa gccggaaact tcattgcctc tattctcgat gagttctatc
     1501 cccaggacga ttaccattct atttattttg gagagataaa agaaagctgg agcagaggtt
     1561 aaaaagctta atcctttatt ccaaaggctt tcaaaaactc cagggggagg gggaaaaata
     1621 ttgttgtagc agtctctgct gagatatcgc ttaaggtttg aagatagcgt aactgtaagg
     1681 caacggggtt tctggcaata atctcagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnag agtagtttgg gaaattagag aagtagcagc tatatagttt tctactttag
     1861 ttattgcatc ctcagggctt agcactcgaa aataaactac cgcgtctaca gtaacaggaa
     1921 cgttatcccg ggtaataaga gactgcttgg gaacatccat agttaccact ctcaggtcta
     1981 ttttaattag ctgatcgata aatggtacga caaagaaaag gcctggtcct tttgctccca
     2041 ccagacgccc cagacggaag attactcctc tctcatactc tcttactact cgaattgccg
     2101 aggctagaaa aataactgca ataactacta ttacccaaat tactaaactc atttttattc
     2161 ctccttttta acaatcaaag tttgtccctc cttctgagag acaatcactt tttctccttt
     2221 tttaaccggg ctttctgctc tggcccacca tagttctccc tctactgtta taaaaccctc
     2281 gggattcaaa tcttctttag ctgtacctac ttctccaatt aaagattcta tccccgttac
     2341 tacctttttc ctcattgttc ttagagccat acctagggca aatacaaaga aagctgcaac
     2401 acaaagtaca gtggttatga taacatttag tggcagcgtt acccctcctt ggatatcaaa
     2461 aagcattaag gagccaaaaa agagtgctac tgcacctcct gcagttagta ctcctattcc
     2521 tggagtaact aaaagatcca gaataaaaag taagagccct aaaaagatga agagaatgcc
     2581 agctaaatta atgggaaaaa tagaaaaagc ataaaaagcc aaaagcaaca atatagttcc
     2641 aaagactcca gggataatag ctccgggatt agaaagctcg aatataattc ccagagcgcc
     2701 aatcactaaa aagaggtaag ctagggtggg attgaggaga aagtgaagga atttttcctt
     2761 ttctttcatc tttacttcta taatctgagg atttttaatc cctaaaaatt ctataatttc
     2821 ttccgggctc tcagcgattc cgtcgatcat acctttttct tgcgcttctt tttcggtgag
     2881 ggaaatgctt tctctaacca ttttttccgc tatttctgga ttacgtcctc gagtttctgc
     2941 taaagttcta atgaaagaag ctgtgtcatt gactattttt tcacttactt cctgtccttc
     3001 cacagttaca ggatgagcag caccaatggt agtgttgggg ctcataaaag cgtgatgaga
     3061 agccataacg ataaaacttc ccgctgaggc agctcggcta ccggggggat agacataggt
     3121 ggttacagga atgggagact ggagaatagc tttaatcatg ttacgcatgg attcctctaa
     3181 ccctcccggg gtgtctaaga ggattagaat gactttagct tcttcttttt ttccctcttc
     3241 tattaaacgg acgacaaatt gctcaataac cgggttaatg attccttcta cttttcctaa
     3301 tataactttt tcttgagctt gtacaagtgg aacccataaa aagctgatga taattagtaa
     3361 tagcagcagg aaggaaaatt tagtttctct tttcatagtt ttaattgtat cataaaagta
     3421 tggtaaaata taatccctca ttccgaggaa gaaaggatgg aaattttttg aatatacttc
     3481 ttgttgctcc gggcacagat aatttacagc ggaaggtgag agttgttcct ttcccccaag
     3541 ccagcctgcc ccttcttgca gctttaactc cttctcatca tgaagtaaaa atcattgatg
     3601 agcgcctgga aaatatagat tttgatggaa aatgggacct ggtaggaatt acagttatga
     3661 ctgctacggc ttatcgggcc tattatattg ctgatgagtt tagaaagaga ggaacaaagg
     3721 tagcgttggg gggtattcac cctacagctt tacctgatga ggctattcaa catgccgata
     3781 gtgtggtaat aggagaggca gaaggcctct gggaagaact tctgggagat ttggaagaag
     3841 gaaaattaaa aaagttttac cgtcatgatt gtcctccgcc tttagcgggg cttccctgtt
     3901 ctcgcttgga tttactagat aagaggtatt tactgagaca tgtcttccaa actactaggg
     3961 ggtgtcccca taattgtgga ttttgttctg tctctacttt tatggggcgc agatatcgtc
     4021 atcgcccggt ggaggaggtg atagaggaga tcggtcatta tcccaatgct cgtatccttg
     4081 gttttctgga tgataatatt gtaggcaatc ctcattactc tcgggaactt tttcaagctt
     4141 taattccttt gcgaaaaaaa tgggcaagcc aggggacttt gaagctggca gaaaatgagg
     4201 agttattgaa actggcagct aaaagtggct gtattgctct ctttgttggt cttgagtcag
     4261 ttaatgaaga taatctaaaa aaaatgcata aaaactttaa ccaggtagac aaatataaga
     4321 aactaataga caggttccat gaatacggca ttatggtaat aggttctttt gtttttggtt
     4381 ttgatgagga tgactattct atttttgatc gaaccctcaa ttttttagaa agtgccaatt
     4441 tagattttgc ccaattttct attcttactc cccttccggg gacagaagtt ttcaagcaat
     4501 ttacagcaga agaacgaatt ttttcttatg attggagaaa atatgatttt gctcatgtag
     4561 tttaccatcc tcttcgtatg accccccaag agcttcagga tggttacaat tacatttctc
     4621 gccagttcta ttccctgccc cggattggaa aaagaactat aagaaactgg agatacgctc
     4681 attatttttt gcccgccagc atttactacc attgggtatg tcgtcatcct aaatcacttc
     4741 ctgaagaagc agcttctgct ccgctttctc taaaagacaa aaaacaatct cccgtagttt
     4801 aggtaagtaa atgaatttaa aaaggcggtg attttatgtc tttaccggca agtgaactag
     4861 aaatcagatt gcaaaaagta agggaacttc ttaccctgaa aaatctttct tgcgggctaa
     4921 tttattatga tgagttaaac attgccaacg gttggtacct ttcagggtgg tgtccccagt
     4981 ttgaaagcgg agccgttctg gttcccgtgc aaggcgagcc gatgatatta ggaggaccag
     5041 agtctgaacc ttttgcccaa attgattcgg ctattaaaaa gaccaggaat attccagttt
     5101 ttatggtacc agaagaggaa taccctaatg cctatatctc ttcttttgct gaagtcttca
     5161 aagaaattgg gatgagtgaa gccgaccggc gggtggggat agttggtttg gagaaaatgc
     5221 cgttgggggt ataccagcga ttgctagcgg agttggaggg agtagaatta gtagacctta
     5281 ctccagagta tgaaaatttt cgaaaattaa aatccccctg ggaaatagaa caaattaaac
     5341 aagcttttgc cattgctgat gaatccataa agataatgga agaattggtg agagaaggtg
     5401 tttttgaaac cgaaatagca ggagctggag aaggaagagc tcgttctttg ggggctaact
     5461 ggtttgcttt taaaaccatt gtagccagtg gaattagaag taatggggta gtacctactg
     5521 cttcgagcga aaagtctctt cagaatggcg agacattgat ggtaggaatt agtcctcgat
     5581 ttaacggata tgcgggagta gcaggttata cttatgtagt gggtaacaaa ataaacgaag
     5641 ctcaaaggac ctgttttaat gatatggtgg aggcttatcg tatcacaaaa tcaaatttag
     5701 tgccaggagc aaaaggacga gacatcgatt ccaagaccag ggaatttctg gtgaaaaaag
     5761 gatatgctaa atatctggtg tgcccatttg tccataccat cggtcttatg gaagcagaag
     5821 cccctttttt tggcccaaac agcaatgata cactgcaacc agggatgaca gtttgcattg
     5881 atgtaagtgt gttttctgta ccagaagtga atgggattcg cttagagagt gggtatttaa
     5941 tcactgaaaa cggagcggta cctctttctc cttttatgga gcaaaaaatt cttaattctc
     6001 gaatatagtt tagatttttc tcttcagaga attttgttag cttatctatt aattaattct
     6061 tttgtggtaa tagctcaaaa aactcgtggt gtaggcaggc cggctttata tggccttata
     6121 aatacaccta ttttcttctt ttctgaagct atcattttct cttaggataa ttggaaggga
     6181 aactagctaa gtactttgta gaccttccta attatgggat tgataagatg cacctgagtt
     6241 atagtgggga ataagcagag aaacgagcgc tgctattccg gttaaaattg ctccccagag
     6301 gtaagcattt tttagtccct gcataaaaat tgaagcctct ttgtctaaca gcttttctaa
     6361 cccttgaagg atggaggagg gaactgaagc actgataatt ccacctcctg tggcagttcc
     6421 cagaaccatt cctacgttac gcactgtggc cagaatactt gaggctacac ctagatgttc
     6481 tcggggtacg cttcccatgg caatgttcat attgggggac tgaaaaattc cagtgcctaa
     6541 gccaaagaga gctaacctcc aggcgatgtc tgctgtacca acatagatgg gtaactggct
     6601 taaagaaaac aagcttagag tgcagaaagt agcacccagg aaagcaagaa agcgagggtt
     6661 aatacggtca gaaagtgaac cagcaaaagg ggctaccact aaagtagtga gggggaaaat
     6721 agttaggaga agccctactt cactttgctc atattgtaac actcgattaa gataagaggg
     6781 agtgagggtt actaagatat actgagacat gaaatttaat agagcacata gattggctaa
     6841 ggaaaaagac aggttatgga ataattgtaa atttatcaga ggcgaatcag cccttttttc
     6901 tattatcaaa aaactgaaga atgccacaag cgcaactaca gcggtaaaaa gagtagtaga
     6961 atttattcct tgagctggaa agcgatttaa aaaaaagaga aaggaaaaaa gaaacaggca
     7021 attggttatt gctcctccca catctatttt tcctggtttc ccttttaatt ctggaataac
     7081 tttatttccc caccacaggc caataatgcc gataggtacg tttatcagga aaatataagg
     7141 ccaaccggca aggtgagtga tgaagccgcc taaggatggc ccaatggcta atccagtgga
     7201 tacactgata gcgttaattc ccagagctcg acctcgctcc tggggaggga aagaggaaac
     7261 aattatagca taaggtacag acatcatcat tccagctgta gttccctgaa tagcgcgaaa
     7321 aagaattaac caggaaatag agggtaaaaa ataggagagg ctgcatagga gagaagtaat
     7381 agtaaaacca gaaagaccaa ttaaatatat ttttttatat cctaaaatat cgcccaagcg
     7441 accatagaac aggagtaaac tgctaacagt caagaggtag accataacca cccattgagc
     7501 ttcagtgagg gcaacaccaa aatgatttcc tataatgtgg tagttaacgt taatcacact
     7561 ggcatcgata ggtcccatga tgccacctaa cattactgcc gttaagattc gataacggtt
     7621 agcatgatgc gatttggtaa gctcttcttc catctatttt tttgggcttc ggtatcagcc
     7681 aggtaggata atttacattc tactatatcc taaaatattt ccattttcca gacttaaacc
     7741 gggaataaat taccatagct cttactatct gatccaaagc tatagctaac cacgcacctg
     7801 taagccccca accaaatgtc attacgaaaa ggtatgccat tgccactctc attccccaga
     7861 tgcctatgat agtggaatag agagggaaac gggtatcacc tgctcctctt agggcgccag
     7921 ccagtataaa ttgagttgac tgggcaggtt gaataagggc tataattctc aagcatatac
     7981 gactttgttc aatgatagcg ctatcattag tataaagcat tgttatatat tttccaaaga
     8041 aaaatattgt aaatgcgctt ataccagaga ctatcattcc caataatctt gtttgacgag
     8101 cactttcttc tgcgatatct ggcttatcag ctcctaaaag ctgtcctaca agagtggtag
     8161 ctgccataga gaaagcttgt ccgggcataa atgataaaga ttggatattc atagctatct
     8221 gatgagtggc aaaggctatg gttcccaatt ctgctactat tcttacaaat gttagctgcc
     8281 ctgctcttaa tacgaattgt tccagggcag aaggtatgcc tatattaaat attcttttca
     8341 aagtgctaaa attcgggcga tagctctctt ttaaggaaag cttaagcact gttttttctc
     8401 tatagagtac ataaaacagg atggctgaag cgacaaatct tgcgatggag gtagctatgc
     8461 ctgcacctgc aactcccagg gccggggctc caagtttacc aaatattaag atatagtttc
     8521 ctaatatatt cgataaattg gcaacaatat cagctataag gggagttttc atgtcaccag
     8581 aacctctaag tatagctccc agcgccattg ctaagacatt aaagggtgtt cctaatgcta
     8641 tcattctcat ataggcgaca cctaaaacca acacctcagg atccgctccc atagctcttt
     8701 ctatttgagg agcaaatata tataaaagag gtagcaacac tatgccgaaa aatatactca
     8761 ccacaatact ctgccttgcc acatcttttg cgctatctat atcttttgct cctataaaac
     8821 gggccactaa tgcagtagtc ccaaccgtaa gagccataaa gacagagata ataagaagaa
     8881 cgggctggtt taccattcct atagctgaga tggcggcggg tcctagtctt ccaaccataa
     8941 tcatatctac catacctaca aaactgataa gaaacatgtt tagcagagca ggtagagaca
     9001 gagaaaaagc cttggagcgg atatgttttt tgtccaagat ttctggcagg atagtattaa
     9061 aatcttctcc ttctaaaaaa tcctcttcat tttctactac tttttcttcc aacttcatct
     9121 tcctttaaga tatattatat tgattcttat ttatataaag agaatgcagt caatttgtca
     9181 aggccaaatt gaactttctt tggaacacgg aaggaagatg gtatcccccg gtaggcatta
     9241 aagttctcag agaaagagaa aaaaatgaga gcttgattta aattgtttca ataatggaat
     9301 atatcaaagt tataaagagg gttgtaatac caagattaag atgctaaaga gaaatattgg
     9361 gggttgaggg aatgcaaagg ttagaggttt actggagaaa gatttcaccg ggattcatgc
     9421 caaggacaga tgtttccaca catgttggca tagagtctct aaaaactata gaaactaccg
     9481 ggaaagatgt ttctcttcag tgttgttgtg gtttttgtag tgaaccgtta gaaatgtgga
     9541 gaaagggata gtagcgagag ctttatttag aaaacgcaat cgtccattac gaaattgtct
     9601 tgcaggataa tgatttacca gggagtaaca tctccgggta gctggggttt aacgagaaga
     9661 gttttttctc gtcggtaagt tacttttcct atctgagagg gaagagtgcg aacatatttt
     9721 acctgagtta caatgacttc tgctctggat tctcctcgaa cagaagagaa ataagcggct
     9781 acccgggctg ctttttgtat ctcttcctcc tcattttcct tgttttttat ttttaaaatc
     9841 acgtgggagc caggcaagtc tcttacatga agccagtagt cctggggaga agcaattttg
     9901 gtgaccaaaa aatggttgct ctttgcattt ttccccaccc atatttcatt gccttgtgaa
     9961 gttttaaatc ttaaagcttt ttctggagaa gaaagaaaag ctttgtttcc ctctttttca
    10021 ggcaggagtt cttcttcaaa aattttctct acttttcttt ccttttcttc taattctttt
    10081 agtctttctc ttagtttgag gtttttttct cggcctttac gataaagtcg aaaataattt
    10141 tgcatattct ggtttatgga aagtcgagga ttaagaggaa taaaaatatc ttgaggagga
    10201 gaagaaaaga cgtttttaac cgttattcct tcttcggttt tgcttattac ttccaagcca
    10261 ggaagaattt tcaggagctc cccttttatc tttaactctt ctaattcttc ctcgtttttt
    10321 atcaaacttt caattttttc tctttccttt tctaagaaat ccagttcttc tttcatcctt
    10381 ttctctttta ttttttgctg aataaactct tcttgccttt cccaccagga aaaatggaaa
    10441 cactccactg cctgattgaa ggaaggaaac tcttgataag aaagagagga ataaggggta
    10501 accgagtccc agaatattcc ctgaggttct ccggaggaag aaaaaaacac tcttatttga
    10561 taattactct ctcgaagggg agaaatagtt tctttcattg cctcccgggg actcttgcct
    10621 cctcccacct tttccgctat tatttggctt aagaaagggc caatgccatc tatttgttga
    10681 acaagctcac gttgtaaatc ctcaggagaa ctggaaaaaa gcaaatcttt ttctttttcc
    10741 tgaaggaaaa agaaggggtc aattttattt ttctggggag gcagagaata tttctccccg
    10801 ggaagaagag tccggaaacg gttctggtct ttaccaaccc gacggtacac tcccaggatg
    10861 tgtgggggtt tactctgatc agtcaaaata gcattagtgt ttcttcccgt tagttctacg
    10921 aacaaataaa gatttccctc ttcaaagagt tggggatttt taatttctat ttttaatacc
    10981 ctgtcccaac ctacttgggt aagagaaatg attttccccc ctaccaaata tttattcaaa
    11041 agctgctgcc agttattagg tttttctttc tgtacttctt tccgagaagt ggtaaaaata
    11101 aaattctcct ggggatagag agaaatgacc agggacttct ctctatcccc aggagaataa
    11161 atctccagcc aggtttctct tctgggagta gtaaaaactc tctgaattcg tgctccttta
    11221 gcttcctttt ctatttcttc tgttaaaaag tgtaatgcca gaccttccaa aatcttcatc
    11281 agttccacca aaaaagtgaa ttatagttta cttatagttt gtcttctcta ttcaggattc
    11341 cgggtgctcc tctccacttg aaaatgagag aaccgagagg tggaagagta agatttaaag
    11401 aaaaagatcg gccgtgcgca ggaatacttt ccgtccacac tcctccccag tttcccagat
    11461 tgctacctcc ataaatttcc gagtcggtat tcaggatttc ttgatagaat ccctcccggg
    11521 gtactcctat gcgataatta aaacgaggaa caggagtaaa attagaaaca aaaactaaaa
    11581 aatcccccgg atttttactt ttgcgcatga aagagattac agagttgtcg gcatcattgc
    11641 aatcaatcca ttcaaatccc tgccaggaat agtccaattc aaagagagat ttttcagttg
    11701 tatagagatg gtttaggtct ttcattaatc tttgaagttg acgatgagtt tcgtagccca
    11761 gtaaaaacca atccagctct tcatcatgat tccattctct ccattgtccg aactctgtac
    11821 ccatgaaaag gagcttcttt cccgggtaac caaacatggt agcaaaactc aggcgcacgt
    11881 tggcaaactt ctgccaccaa tctcctggca ttttatcgat tagatttctc tttccatgca
    11941 ctacttcgtc atgagaaatg ggtaaaataa aattctcaga aaaagcatag agtaagggga
    12001 aggtaagatt ttgatgatgg tgttttcgat aaacagggtc tttactaata taagtgagga
    12061 aatcgttcat ccatcccata ttccatttga aaagaaatcc taatcccccg agatatgggg
    12121 gtagtgttac tccaggccag gcagtagatt cttcagcgat ggtggcgatt ccaggaaaat
    12181 agtgatagat attttcatgc aaataatgga ggaaactaat agcttctaag ttctcctttc
    12241 ctccgtaagg attaggtatc caatctcctt catttctacc ataatcgaga taaagcatag
    12301 aggctactgc atcaactcgc aatgcatcaa tatgatattc tttgaaccag taaaaagcgt
    12361 tggagattaa aaaagttctt acttcgtttc ttccatagtt gaaaatataa cttccccaat
    12421 gggggtgttc tccccgtcgg gggtctaaat gttcgtacag tgcggtacca tcaaatcttc
    12481 ctaagccatg ggcgtcttta ggaaaatggg caatgaccca gtctaatatt actcctattc
    12541 cttcctggtg acaataatct acaaaatatt gaaaatcttc tggagtgcca aagcgactgg
    12601 tgggagcaaa atatccggtt acctgataac cccaggaggc gtcaaaaggg tgttcggcga
    12661 tgggaagaag ctctatatga gtgaatccca tctctttgac ataggctacc agttggtgag
    12721 ctagatctct atagtttaaa aaagaattat ctttccctct tttccaggag cctaaatgaa
    12781 cttcataaat actccaggga gaggaaagaa agttttgaga ttttcttttt tccatccact
    12841 cttcatcttt ccagggaaaa ggagaaagat tgtacactat agaagcagta gcggggcgta
    12901 attcagagaa gaaagcatag gggtcagctt taagaaggag attaccttcc tgggttttga
    12961 tttcaaactt atattttgct ccttcttctg cttgagggac gaatatctcc catattcctg
    13021 agcttcccaa tactctcatt tgatgaatac gtccatccca cctgttgaaa tctcccacta
    13081 cgcttacccg gcgggcatta ggagcccaaa cgctaaaata gactcctttt atgcctccta
    13141 cctctatcag gtgagcacct aaccgttcat aggcaaagtg ataagtgccc tcgttaaaaa
    13201 gatggaggtc gaattcagaa aaaaaaggaa ggaaagagta gggatcagcg gtaactctta
    13261 ttccttcttc ttcctctatt tctaaaaagt aggcaaaaaa atctttccca ggaagagtca
    13321 gctcaaaaaa ccctctttct tctactttat tcataagcca gctattctgg ctgttatatc
    13381 ggtctattac ttttattttt ttagctgcgg gaataaaaac tcttacacaa actccctttt
    13441 cttcttctaa aggatgcatt cccagtatag aaaaagggtc atgacaatcg gcattgatta
    13501 tagactccat tttttcttta cttatagtaa cgggtgaatt actcatactt ttacctcctt
    13561 ttgggaaacg gtacgccagt attcgtgatt tatttctttc agtatttccg ggtgaacctg
    13621 atagggggtg attatttttt taggaggaag agaaagagaa agctgaggaa aagggtaatt
    13681 tcttagtttt tcattgtagg caaaaaccat caatttttcc aggttgaccg tgtctgcaac
    13741 gttataggct aaaagtgttt ccagtgcctc aggacacccc tcccggtatt tttgccatag
    13801 tagcactgcc gtataaccat ttactcctcg caagtctcct ctatcgatgc ctatggcttt
    13861 ttcacaagct tttagtcctc ctcgaaagcc caggcttcgt aaaataaagc ggagatctaa
    13921 atgtacgtgg ggaaatttta tccctaaatg ctgctccaga aacggaatat caaaacattt
    13981 gccattgaaa gttacaatga ctttgtattt cataatctct tccttgaatt ccggtagatt
    14041 gattccctga ataaaagttt ttattctttt tccatcaaaa atagttatgg cagtgacata
    14101 gtcctgagga gggtttaatc cagtggtttc aatatctaaa aatacggtat cattttgaaa
    14161 gtgagggaac aacctccagt ggtcatgagt atctaaatgt tccttaaaaa agcgagcttc
    14221 tcctccttcc aatttttgaa gggagcgata aaggaaagag cgcgctgaac gggaaaactt
    14281 agaagggaca atcttttcca gagcgggata acctaaggct tcttcccagg tagtgatgcc
    14341 ctcttcccag attctccttt ctattttttc cccgacaccg ggaatgtgac aaaaagtttg
    14401 taaaagcatc ttttattccc tcaattcttt ttagtaaaat ggaaagattt ctgtggtttt
    14461 atttctttaa cgggcatttc aaaaattatg tcttcctgta attcctgaaa acgaggatgt
    14521 cgaaaagcgc atagtattcc tcttttaccc aagaggtgat ggatttcagg atgcaaaaat
    14581 tctaaatcct tttcttccag cacagtagtg gaagatattc ctcctccgct ccatcctggc
    14641 caaaaatcaa ttaagctttc cagtatttct ccccgatcgg agagaatgaa aagctctact
    14701 tctccactgt tctcaaaaag accggaaatc tttaccggat agtagaaatt tcgggagagg
    14761 aaaatataac ccacaggttc aaccctctcc ctgttttctt gaaaatccac aatatccagg
    14821 acaaaataat ttattcctct ttttaagtaa tcatcgacta ggttttgtaa tttttcctcc
    14881 tgaaagcgag agggaaggtt attctgaagc aagaaacttc tgacccattc caaaaattct
    14941 tcgctatcat ttacttttac caccgtaatt ttatgagctc cttgagtaac ctgatattcc
    15001 acttttattg cttcttcttt ttctcccccc ttataaaatg ttagatattg caaattatgt
    15061 ttttcacaga tatgagaaag tctcttccat atctcgttgt ctacttctat tatttcaggg
    15121 agggagggaa aaggaataag gcgtaaaaca ggagtatttt gagaagcaat gaggtcggta
    15181 gagagaataa taatttcttc aaaaccgtcg tgagcaataa tggctttttg ggccacctct
    15241 tcaaccttca ctccccctgt tggaactatg gctcccatgt cggcataaag aggagtggtt
    15301 aaaattgcaa gaaaggagat gagtattgtt gtcagggctt ctttcattgt ttgctccttt
    15361 ctattccgtt acctattata ttgtaagata atttaacata ttttccaata gctaactata
    15421 ttacagcaat attatgtact aaacgttgtc ataaggttct tttcaaaaat cttgtgtgac
    15481 aggttagtta ttcgaaagct tttctaaagt ggatagaaag atcggaaaat actgcttccc
    15541 gcagagaagg cgctcggagt tagattattt tcctggttaa tttagcattt ctttgtatta
    15601 ctcatccggt ttaccaccag tttccggcag tcttttatat tcccaacccg ggttctttca
    15661 caatctttag ggggccagat acaccaacta cccatctcgt ttttctttgt tgtttcctga
    15721 gtaaactgct cggctatttt ctgtttccca ccagggagag gcttttttgc tccacttgca
    15781 atcgttgcct gtaattcctg ttagtttcag actctttgta agtgaattga ttattttaag
    15841 ctggtgaagg cagcaatatt cttttcacaa atttttatca gcgtttcgat ctggcttaga
    15901 acttctattt ttgctcttct cgctcgtatt tatcccagaa acttttcttt cgagcatgag
    15961 tctcggctat ttttttaaag tacagctcat gaggaagaac cccctgccaa tcagagactt
    16021 gaggggctaa cttctccaat tttttcaaat accgcacccc gtattgataa tatttggatt
    16081 gggctcggtg cagaatagac tctaaaagtt ctcgatagat aatagtggag acaaggaatc
    16141 gattttcttt ttcaaaccgc tgagctagag ttagcattag accgtattgg tcgccattta
    16201 aagtgtcctc attttctaat acatattttt gagccaggtc aacctggtta gtatcgagca
    16261 aaaaggaaat atcataatag aaagaagaag ggttagccat aatttcctgg ctggtttcag
    16321 ctatcacttt ttctcgttgg tcctcaccga taatagataa aagttcttct aggttatcga
    16381 cgcctttgtg ctggcgaaaa attttccagg ctacttctgc taatttttcc tggttattgg
    16441 tttttcgata aatatctaac aataatttat ctcgtttata gtcatctaag gccatttctg
    16501 gtggaacttt ctttatccag tcgagtgctt tttctgactc ttgcgcttct aaataaactt
    16561 cagcaatatc taaacaagtt ttagaagaaa gatcaggcca ggtagctaat gcagctcttt
    16621 caaaaagagg agcgtcatgt agctgacgag ctaaagattc tactgcaaag agagaatgtc
    16681 gagcatcgta ttggttatct ttaacagtct tatcaatatt ttcggcattt tcccagaacc
    16741 gttgagccag agagcgcaag gtttcctcgg gtaaaaattg gaaagcatgg tctaccaatt
    16801 cttcccggac cccgtattca tcttcctcat agagcttgaa aactaagtcg gacaaatatg
    16861 atttatcttc tatatctcga gcataatgag taaaaagttc agtggcatcg tagcgaaata
    16921 cctctcccac atttccgtag gaatcatcac aggactctaa aactttgtta tcactttcaa
    16981 agaaagctac tactaactcc aacccgattt tagggtcttc aatgtttctt tgaatatcag
    17041 ctaaaagatc acttaaatct gaagcataac tctgagcttc tctggctcct aaaaaccttt
    17101 ttctttgcct taaccctctt attctggctt taaaacggcg gagattttct cggggaggag
    17161 ataatagttg ttcaacgagc tgagcagctt gatcgctgcg ggtggacaaa tctagaaggg
    17221 catcagctaa cttctctgcg cccaatttaa tcagttgttc tctagggtta ttaggtatca
    17281 ttaaacaaac gtgacctcac ttgggtaact taaattaatt tttattttat cactttttat
    17341 ctttggatgc ttttgttgtg ttatatttat tctgagatta ttgaataata ggaggtaata
    17401 aatgaagaac aagctgatag tagcaggagt aatttttacc tgtttggttc ttctggtagg
    17461 ttgtggtgat gaaaaatgga accctttaga tgtggagata gttgaccatg agacgggatg
    17521 cctcgcttta gaagaagagt ctatcaccac tctctgggag ggagaagata tcgtagctac
    17581 cactattttc tccacgtctg ttccttgcta tcaaatacgg tccatttctg ctcgagagaa
    17641 ggaggataaa atcgaagtaa agataaaact ggagcgagaa gcaggggcgt gtatagaatg
    17701 tataggcttt cagaaaataa cttttcgaat aaaaaatcct cgcctttatc cggaaattaa
    17761 cttggaattg caagtagaag ttgatggcca ggtattgatt caaaccatta tagaacagcc
    17821 tgcgacgtaa acaaaaaagc aaaatatata acaaaagaat tcgaacaatt acgctggaga
    17881 aaaagcactc cagcgctcct tcggcgtaag gtagtggttt ttcggttaga taaaatatct
    17941 tcttgtttag aaaggaagaa aaatggaaaa gttaaaccaa cttttgctta atcgggtggc
    18001 tattattacc ggaggcactc gaggaattgg tctgggtatt gccagggttt tgagtcaagc
    18061 aggagcgaaa gtggcattgg tttatcggtc agataatcag agagctaatg aggctataga
    18121 agagcttaag aaagagggct gcgaggctat atctattcaa ggagatgtag gcataaagaa
    18181 agaagccgaa agagtggtta gcttagtagc tgagaagtgg ggaaaggtag atattttggt
    18241 caataactgt ggaattttag attttacttt tttagaaaat ttaactgaag agcaatttga
    18301 ccaggtattg agagccaatt taaaaagcgc tcttttttgt acccaggcag tattgccctt
    18361 tatgaaacag gaaaagtttg gaagaatcat aaacgctagt tctatatctg gccactttgc
    18421 tgatgtagga cagattgctt acggtgtgag taaagctgga gtagagatgt ttactaaaat
    18481 tgcctcagct gagttagcgc cttatggtat tacagtcaat gcttatgctc cggggattat
    18541 cgaaacagat atgactcggt ctatgattga agagagagga catcttcaga aagagcaaat
    18601 tcctctgaat agatttggaa ctccggatga ggtaggagct ttagtgcttt tcctttcgct
    18661 ggaggaagct ggatatataa ctggggagat aattggagta gatggaggaa tgctcaaagt
    18721 gcaaaatgcc tggagagctc gctacgaggg aaaagagtga gaataaaaaa ttgaaggtag
    18781 tatgttcaat atattcgcaa aataacttga ggagtggata acattgaaaa aacagttttt
    18841 aatagtccct ttctttcttg ctctcatagt tattggcttg ggtctttcag ggatgggtgc
    18901 ggtaatcccc gaaatggctc aacagttttc cgtttcttat tctgtagtgg gaagagtttt
    18961 cttgttccat ggcttgggct attttgtctc tattcttctt gctgggattt taggagatat
    19021 tattaaccaa gtttctatcc tgaggttagg aattttccta tctttagctg gttttggagg
    19081 gatttctttc cttccttctt ttcctttagt tatggcttct tttttgctta tgggagtggg
    19141 gttgggattt attgatgcta tgattagccc cgtagctaat agtgttttta ctgaaaaacc
    19201 tgccactacc ctaaatatca ttcacgcttt ttttggatta gggtctttag ctgctccccg
    19261 tctttactct tttttagtag tagagggtta tgggtggcgg aatctttacc aaatatgtac
    19321 tctctttact ctgataactt tggttatttt tctttttcct tttattcctc gaaaaaatca
    19381 gccttctcaa tttaaggaat tgttaggagt gtttaagcag ccggcgttat ggattatggg
    19441 tttcactact ctcttttacg cgggaggagt gagtacctta aatggctggt tagtgtctta
    19501 tttagaagga gaagggatag taagaggaga aggagccatt tttctctctt atttctggtt
    19561 gggtataatg ttagggaggc ttatcctttc tccagtttcg gataaagttg gtcatctcaa
    19621 tactattcgc cttaatgctt tagggggaat agtgtttgtt tccttgtttt tgcttttacc
    19681 ttttcattct acttctgctg tgcttttgct ttttggcact ggttttatgc tctctacttt
    19741 gatacctacc accgtttctt atgccctggc gacttttcca gaagtaactt ctactgcttc
    19801 cagttgggtt ctatttaaca atagtttagg tagtttccta ttcccctggt tgggaggagt
    19861 aatagggaca gcttttagtc tgcgcctaac tatgattttt gttcccctat ttctcatttt
    19921 gatgttttta tttcagcaat ggttagcta
//
LOCUS       DAOPSS010000015        19336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2402_length_19336_cov_8.455319, whole genome shotgun sequence.
ACCESSION   DAOPSS010000015 DAOPSS010000000
VERSION     DAOPSS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2402_length_19336_cov_8.455319"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..683)
                     /locus_tag="PKN31_01830"
     CDS             complement(15..683)
                     /locus_tag="PKN31_01830"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOA98703.1"
                     /translation="MRRKSFFLLTIFGLILVILVAGCSNSINTPETNFGISSSPTSSE
                     IRDKFIEFSDSRIVLDVPFLPQVPPGDWVNTRNCGPACAVMMRAYYFNIQPSPDHIIQ
                     ADNWLNAHFGLPVNGYNGDWTNIFQVRAWLDSEGVPTKVGMGNLEQARAMLSEGKPFL
                     VAVYSNMNPNGGAKHAMLVVGIDSDNIYVNDPGKIAGANNSYPLSQFLSAWSAQGNWY
                     LALN"
     regulatory      complement(773..858)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1049..1858)
                     /gene="truA"
                     /locus_tag="PKN31_01835"
     CDS             complement(1049..1858)
                     /gene="truA"
                     /locus_tag="PKN31_01835"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOA98704.1"
                     /translation="MVLEYKGSNYQGWQRQSRGKTIQGVVEEVGEGILREKITLFASG
                     RTDAGVHAAGQVASFTTSTYMSTEAIKKAFNSLLPPDIRVVDVLEAPLSFHPRKTAIR
                     KIYIYVFSLMYPPSVFWKPFVYSFSPKNFNLARVKECLSLLEGTHDFTSFSSSGGADF
                     SGVRTIYSIEIKEKPSGLLQLQVEGDGFLYNMVRIIAGELWKVGMGKKNPEEIKFMLE
                     NPSRSFNRLALPPRGLFLIQVCYPPDFNPYQNLELVDEGWIVPLWQEKKSI"
     gene            complement(1879..2679)
                     /locus_tag="PKN31_01840"
     CDS             complement(1879..2679)
                     /locus_tag="PKN31_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HOA98705.1"
                     /translation="MWGRSLAIGQYVPLDSPIHRLDPRVKIVFVGISIINLFILQDWF
                     SFLLWGIALIFLVSLSHISFTYLLKSLRPLLFLLILTLVLHFFFTPGEYIWEYGFLHI
                     SREGVSKGLLIVTRLCLLILVTSLLTLTTSPVELTDGMEYLLSPLKRWGFPAHELAMM
                     MTIALRFIPTLLEEADRIMKAQMARGMNLDSGGIMQRAKNLIPLLVPLFVNSFKRADE
                     LAIAMESRCYRGGKGRTRLRVLELTSSDYYFLVLSFSFIALLSLRSFI"
     gene            complement(2669..3541)
                     /locus_tag="PKN31_01845"
     CDS             complement(2669..3541)
                     /locus_tag="PKN31_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOA98706.1"
                     /translation="MFIKLENISFTYLPGTPLAVTALEDIDLEIERGESIGIVGRTGC
                     GKSTLIQHFNALLIPQSGRVLVDGVDTGKKGAPLQEIRRKVGLVFQYPEDQFFEETVF
                     KEVAFAPRNLGVEGKELEDKVKWSLEAVGLDFEAVKDKSPFELSGGQMRRVAIASILS
                     MEPEALVLDEPTAGLDPEGKRIILGQLEKLQKERGLTLIIVSHSMEDLARVAKRIIIM
                     DKGKIKFDDDIRQVFSQVEWIKSLGIFPPVTAQVAWLLRERGFDVTPGVLTPQELAEE
                     IQLKVLEGKRVNVG"
     gene            complement(3532..4362)
                     /locus_tag="PKN31_01850"
     CDS             complement(3532..4362)
                     /locus_tag="PKN31_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005955923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOA98707.1"
                     /translation="MIEFKKVSFWYGEEYESEARNEEILREVDLEIHQGKFMVLLGRN
                     GSGKSTLAKHTNGLLLPKSGEVIVDGMNTRDEESIWEIRRRVGLVFQNPDNQIIATTV
                     EEDVAFGPENLGLPREEILARVEEALTLVGMTEYRNKEPHLLSGGQKQRVAIAGVLAM
                     HPEYLILDEATSMLDPEGREELMKVIASLREKIAVLHITHNVEEAIEADEVLVMDEGK
                     IVARGKPRKIFSQVENIEEWGLELPQVTETALLVKKQFPSLFKTLPLSPGELVEELCS
                     "
     gene            complement(4389..4742)
                     /gene="rplQ"
                     /locus_tag="PKN31_01855"
     CDS             complement(4389..4742)
                     /gene="rplQ"
                     /locus_tag="PKN31_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOA98708.1"
                     /translation="MRHRRRTEKLGRTTAHKKSMMSNLLVSLIKNEKIQTTEAKGKAL
                     KRHFDRVVSLAIKGDNASLREVISYLRDKEAFKILLREVAPRFRERRGGYCRLVKMGY
                     RRGDGAPLVLVEIVE"
     gene            complement(4720..5739)
                     /locus_tag="PKN31_01860"
     CDS             complement(4720..5739)
                     /locus_tag="PKN31_01860"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOA98709.1"
                     /translation="MLDIRDITRCDLLEWEEDHATKVQYGKFSIEPLEVGWGITVGNA
                     LRRVLLSSIEGAAVTGVQIEGIIHEFSSLPGVREDIIDIVLNLKGLVLRSYSDDEETL
                     YLEVEGVPGELREVTARDIQPNSNVEIINLDHHLAEIDEEAKLSMKISVKKGRGYLEV
                     EGRDIKEDYNFIPIDALFTPVKKVNFQVLDTRVGQLTNYDKLIIEIWTNGAITPQQAL
                     SQSLDLLAEEFSRFGGLLKEKIGESEPAEEVEEDEETSEAETSIEELELSVRAYNCLK
                     RAHIDTVQELVSILQERPEDLKKIKNLGQKTFEEIVEKVEKWSSEVESKEEKGVNKGE
                     APAED"
     gene            complement(5756..6376)
                     /gene="rpsD"
                     /locus_tag="PKN31_01865"
     CDS             complement(5756..6376)
                     /gene="rpsD"
                     /locus_tag="PKN31_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOA98710.1"
                     /translation="MSRYTDAKCRLCRRHGSKLFLKGPRCYTDKCAMEKRPFPPGVHK
                     GSRRRLSSYGIQLKEKQRVRFMYGITENQFKNYFEKASRLPGVTGENLLSLLERRLDN
                     VVFRAGWADSRADARQMVLHGHFLVNGRKVDIPSYLVDEGDVVELSSRGEKHKKIKEL
                     MEEERTPTPAWLGVSEDGKATILREPQREDIDYAVEEQLIVEFYSK"
     gene            complement(6392..6778)
                     /gene="rpsK"
                     /locus_tag="PKN31_01870"
     CDS             complement(6392..6778)
                     /gene="rpsK"
                     /locus_tag="PKN31_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOA98711.1"
                     /translation="MAKAGRKKKREKRTVREGIAHIKSTFNNTVVSITDKEGNVLSWS
                     SAGSAGFKGTKKGTPFAAQLAAEQAAKKAMDQGLREVEVLVKGPGSGRETAIRSLQAA
                     GLIISSIKDVTPIPHNGCRPPKQRRV"
     gene            complement(6814..7182)
                     /gene="rpsM"
                     /locus_tag="PKN31_01875"
     CDS             complement(6814..7182)
                     /gene="rpsM"
                     /locus_tag="PKN31_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOA98712.1"
                     /translation="MARIAGVDLPNHKRVDIALTYIYGIGRKLSAKILEEANVEPGIR
                     VKDLSDDELVRIQKEVEKYPVEGELRAQVSQNIKRLISIGCYRGMRHRQGLPVRGQRT
                     RTNARTRKGPRRTAGAKRGR"
     gene            complement(7197..7310)
                     /gene="rpmJ"
                     /locus_tag="PKN31_01880"
     CDS             complement(7197..7310)
                     /gene="rpmJ"
                     /locus_tag="PKN31_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOA98713.1"
                     /translation="MRVSASVKPRCEKCKVIRRRGKVMVICSNPRHKQRQG"
     gene            complement(7327..7545)
                     /gene="infA"
                     /locus_tag="PKN31_01885"
     CDS             complement(7327..7545)
                     /gene="infA"
                     /locus_tag="PKN31_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOA98714.1"
                     /translation="MVKEDIVEVKGTVLEALPNAMFKVELETGKVILAHISGKMRMHY
                     IRILPGDKVTVQISKYDPTKGRIVYRYK"
     gene            complement(7545..8306)
                     /gene="map"
                     /locus_tag="PKN31_01890"
     CDS             complement(7545..8306)
                     /gene="map"
                     /locus_tag="PKN31_01890"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOA98715.1"
                     /translation="MSKLISDDEVEKIRQSGKILAEVIREIKRLIIPGVKTSFLDQVA
                     ADLIKSHGARPAFLGYRGYPASICISINEQVVHGIPGERVIESGDLVSVDIGVEFQGF
                     YTDAAFSVVAGDGNSLAQKLVEVTEKSLYEGIKRALPGNRIGDISHAIQETIESAGFS
                     VVRDFVGHGVGRALHEEPQIPNFGKKNRGVVIEEGMVLAIEPMANEGGYQVEILDDDW
                     TVVTKDGGLSAHFEHTVWVTKKGPVILTHSGDKEQ"
     gene            complement(8303..8962)
                     /locus_tag="PKN31_01895"
     CDS             complement(8303..8962)
                     /locus_tag="PKN31_01895"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOA98716.1"
                     /translation="MRIVLLGPPGAGKGTQAKMMEKDLGVSTLSTGDIIRLAIKNQTP
                     WGKKAEEFVKSGRLVPDDVVIGIVKEKIDQDNLWSGFILDGFPRTIKQAEALEEMLKE
                     KGLSLDVVLYFAVDKEEVIKRLSSRRVCENCQTPYNLLSNPPQRDELCDRCGGKLIQR
                     DDDKPEVIRKRLETYEEETKPLIDFYEERNLLRVIPSSAPIEEVYDRVKEALKEVAAG
                     Q"
     gene            complement(8979..10247)
                     /gene="secY"
                     /locus_tag="PKN31_01900"
     CDS             complement(8979..10247)
                     /gene="secY"
                     /locus_tag="PKN31_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOA98717.1"
                     /translation="MWESLLKLFRIPDLKRRILFTLFMLVLFRVGAHIPVPGIDSRAL
                     ADVFSQGGILGFLDMFAGGALSNFSILALGIVPYINASIIIQLLTAVIPSLEELSRSG
                     EEGRDKIAQYTRWFAIVLALVQGFGITSWIEGMGVVLYPGWGYKITVLLTLTAGTIFL
                     MWLGELITDLGVGNGTSLIIFAGIVARVLPQLVRTFSLIKVGEINLLYFILSLVMNVA
                     IIAVVVAFQEAQRRIPVQYSKRVVGRKMYGGQSTHIPIRIVQGGVLPIIFASSILLFP
                     ATLAQFFQGSKVMASIASALSPNSPLYLSLYAALIIFFTYFYTAVSFNPVELAENMQK
                     YGGFIPGIRPGRPTVEYLDRTLSRITLWGALFLTFIAIVPNIITDLTKVTTFYFGGTA
                     ILIMVGVAIETVRQLEAQLLMRHYEGFIRK"
     gene            complement(10249..10689)
                     /gene="rplO"
                     /locus_tag="PKN31_01905"
     CDS             complement(10249..10689)
                     /gene="rplO"
                     /locus_tag="PKN31_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOA98718.1"
                     /translation="MGVNDLKPRDGSFHYRKRVGRGISSGHGKTSGRGHKGQKSRSGG
                     KIPPYFEGGQMPLVRRIPKRGFRSWNRKEWQIINLEALNRFEEGEEVNKELLRKAGLI
                     KKLDLPVKILGGGELTKKLIVKADAFSLSAKEKIEEKGGRAEVV"
     gene            complement(10691..10900)
                     /gene="rpmD"
                     /locus_tag="PKN31_01910"
     CDS             complement(10691..10900)
                     /gene="rpmD"
                     /locus_tag="PKN31_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007203533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOA98719.1"
                     /translation="MGEKLLKITLKKSPIGRPESHKRTVKALGLRKLNQTVYHKDSSS
                     LRGMVNKISYLLEVEEVESAEEGER"
     gene            complement(10914..11423)
                     /gene="rpsE"
                     /locus_tag="PKN31_01915"
     CDS             complement(10914..11423)
                     /gene="rpsE"
                     /locus_tag="PKN31_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOA98720.1"
                     /translation="MRRIKPDGLELKERLIEVNRVSKVVKGGKRFGFRALVAVGNGEG
                     VVGIGTGKAKEVPEAVRKATEKARKSLIQFPMRGTTITHELVGRAGASRVVLRPAAPG
                     TGVIAGGAVRAVLEVAGIKDVLTKSLGSNNPVNLARATMQGLMSLKDPQEVARLRGKE
                     VKDFFTVSR"
     gene            complement(11442..11804)
                     /gene="rplR"
                     /locus_tag="PKN31_01920"
     CDS             complement(11442..11804)
                     /gene="rplR"
                     /locus_tag="PKN31_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOA98721.1"
                     /translation="MMLVEEKREKRDKRHRRVRKKVRGSAERPRLVVFRSLRHIYGQI
                     IDDSEGRTLVAVSSLTSELRGKDGSGVEIAKEVGKLLAQKAREKGIETVVFDRGGYKY
                     HGRVKALAEGAREGGLQF"
     gene            complement(11817..12353)
                     /gene="rplF"
                     /locus_tag="PKN31_01925"
     CDS             complement(11817..12353)
                     /gene="rplF"
                     /locus_tag="PKN31_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOA98722.1"
                     /translation="MSRVGKKPVVIPDGVNVKIEGRTIVAKGPLGTLEREIHPRVKVE
                     KEGNTLQVIRKGEDKLDRSLHGLTRTLVANMVEGVSQGFEKALEISGLGYRAQKKGQN
                     LVLNLGFSHPIEIEPPSGITFEVEGQVVKVKGIDKEKVGQVAANIRGLRKVEPYHGTG
                     IKYVGEVTRRKQGKATAK"
     gene            complement(12367..12768)
                     /gene="rpsH"
                     /locus_tag="PKN31_01930"
     CDS             complement(12367..12768)
                     /gene="rpsH"
                     /locus_tag="PKN31_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOA98723.1"
                     /translation="MAHTDPIADMLTRIRNALKAGESIVKVPSSRSKIEITRILREEG
                     YINDYKVLTQEGGKSDLIIELKYGPNRERVINGLKRISKPGLRVYAGKNEIPVLLGGM
                     GTVVVSTSQGMMTGKEAKKLGVGGEVICYIW"
     gene            complement(12784..12969)
                     /locus_tag="PKN31_01935"
     CDS             complement(12784..12969)
                     /locus_tag="PKN31_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOA98724.1"
                     /translation="MARKAKIEREKKDLKFKVRYRNRCSLCGRPRGYLRDFGLCRICF
                     RNLASRGEIPGVTKSSW"
     gene            complement(12983..13522)
                     /gene="rplE"
                     /locus_tag="PKN31_01940"
     CDS             complement(12983..13522)
                     /gene="rplE"
                     /locus_tag="PKN31_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOA98725.1"
                     /translation="MSITKEKYQKEVIPSLVETFGYRNVMQVPKVEKIVINVGVGDAT
                     QNSRYLDLAIEELALITGQKPLVTRAKKSISNFKLRRGAPIGCKVTLRKNTMYEFMDR
                     FLNIAVARIRDFRGFPDSSFDGRGNCTIGIEEQLIFPEISYDRVERVRGMNITFVTTA
                     STDEEAKALLEALGLPFRK"
     gene            complement(13540..13887)
                     /gene="rplX"
                     /locus_tag="PKN31_01945"
     CDS             complement(13540..13887)
                     /gene="rplX"
                     /locus_tag="PKN31_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOA98726.1"
                     /translation="MESREKKASVAIKKGDSVEIVRGKNRGKRGKVLKVFPREGKVVV
                     EGINIVKKHTRPTQDNPQGGIINRENPVKISNLRLICSRCNQPTKIRRVKVAESRSRV
                     RVCKKCGEIIDKV"
     gene            complement(13902..14270)
                     /gene="rplN"
                     /locus_tag="PKN31_01950"
     CDS             complement(13902..14270)
                     /gene="rplN"
                     /locus_tag="PKN31_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOA98727.1"
                     /translation="MIQSRTMLEVADNTGAKKIMCIRVLGGSGRRYASIGDVIIATVK
                     EAEPRSDVKKGEVVRAVVVRTKKEVGRPDGSFVRFDDNAAVLVTNQDVPRGTRIFGPV
                     GRELRSRNFSRIISLAPEVV"
     gene            complement(14245..14535)
                     /gene="rpsQ"
                     /locus_tag="PKN31_01955"
     CDS             complement(14245..14535)
                     /gene="rpsQ"
                     /locus_tag="PKN31_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOA98728.1"
                     /translation="MGARKRLTGEVVSNAMDKTVVVKVVRISEHPLYRKKIRKASRFS
                     AHDENGICAVGDRVLIEETRPLSKTKRWRVVTVLQKARVEGGEEVDSVENYA"
     gene            complement(14537..14749)
                     /gene="rpmC"
                     /locus_tag="PKN31_01960"
     CDS             complement(14537..14749)
                     /gene="rpmC"
                     /locus_tag="PKN31_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOA98729.1"
                     /translation="MKALELRNLTNEELNQRFKDLRTELFNLRFQSITGQLGNPKRIK
                     MVKKDIARVKTILRERELSEEKQGVK"
     gene            complement(14761..15171)
                     /gene="rplP"
                     /locus_tag="PKN31_01965"
     CDS             complement(14761..15171)
                     /gene="rplP"
                     /locus_tag="PKN31_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOA98730.1"
                     /translation="MLMPKKVKYRKQHRGRMKGVASRGNKVDFGDFGLQALEPAWITN
                     PQIEAARVALSRSMKKGKIWIRVFPDKPYTKKPTETRMGKGKGPVEGWVAVVKPGKIL
                     FEIGGVDRETAQEALRLASHKLPIKTRVVERSID"
     gene            complement(15149..15895)
                     /gene="rpsC"
                     /locus_tag="PKN31_01970"
     CDS             complement(15149..15895)
                     /gene="rpsC"
                     /locus_tag="PKN31_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOA98731.1"
                     /translation="MGQKVNPIGLRLGIVKDWQSRWYAEKNKFKDYLIEDLKIRQEVK
                     NRFYRSGISRVEVERLANQIKIIVKTSRPGIVIGRRGSEVEKLRQDIQNMTGNQNIQI
                     SVEEVPIPEIDAQLVAENVASQIERRVSYRRAMKQAINRALRMGAQGIKISCSGRLAG
                     AEIAREEWYREGRLPLHTLRADIDYGFTESLTTYGKIGVKVWIFKGEVISPGELLEAE
                     GAALSGKEKKEKEEGSYEEVAEDYVDAQES"
     gene            complement(15896..16237)
                     /gene="rplV"
                     /locus_tag="PKN31_01975"
     CDS             complement(15896..16237)
                     /gene="rplV"
                     /locus_tag="PKN31_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOA98732.1"
                     /translation="MEVKATAKYLRISPRKARQIVDLIRGKSAQEALTILQFIPKKSA
                     EMVYKVLKSAVANAENNYNLDVDQLVVSKAFVDQGPTLKRVRPRAMGRGYLIRKRTSH
                     ITVAVSEKEEQ"
     gene            complement(16254..16538)
                     /gene="rpsS"
                     /locus_tag="PKN31_01980"
     CDS             complement(16254..16538)
                     /gene="rpsS"
                     /locus_tag="PKN31_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOA98733.1"
                     /translation="MGRSTKKGPYVDSRLRERIEELNRKGEKRVIKTWCRACVIIPEM
                     VGHTIAVHNGKKHIPVYITEQMVGHKLGEFAPTRTFKGHGSPTERSTALR"
     gene            complement(16541..17365)
                     /gene="rplB"
                     /locus_tag="PKN31_01985"
     CDS             complement(16541..17365)
                     /gene="rplB"
                     /locus_tag="PKN31_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOA98734.1"
                     /translation="MANLKQYKPVTPGRRHMSGHTFEEITPGGPEKSLLKPLKSKAGR
                     DNQGRISVRHRGGGHKRKYRCVDFKRDKFGIPAKVERIEYDPNRSARIALLKYADGEK
                     RYIIAPLGVTVGDVLMSGEEADIRPGNALPLRNIPLGTFIHNVELRKGKGGQLARSAG
                     AYAQLMAKEGDYAQVRLSSGEVRLINIDCLATVGQVGNVEHENVAIGKAGRKRWLGVR
                     PTVRGVAMNPIDHPHGGGEGKSHGGRHPVSPWGVPTKGFKTRKNKKTDKWIVKRRK"
     gene            complement(17381..17668)
                     /gene="rplW"
                     /locus_tag="PKN31_01990"
     CDS             complement(17381..17668)
                     /gene="rplW"
                     /locus_tag="PKN31_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOA98735.1"
                     /translation="MSNQREILINPIITEKTVNLQKESKYVFKVDSRATKAEIKKAVE
                     DAFGVTVTRVNTVRVKGKTKRLGRFEGKTSSYKKAIVFLKPGERIKAFDIT"
     gene            complement(17661..18287)
                     /gene="rplD"
                     /locus_tag="PKN31_01995"
     CDS             complement(17661..18287)
                     /gene="rplD"
                     /locus_tag="PKN31_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOA98736.1"
                     /translation="MELEVWNKEGKVVDTITLEDTVLSDEDYHLLYLSVKAYLDNQRL
                     GTAKTKTRGEVRGGGRKPWRQKGTGRARHGSIRSPIWKGGGVTFGPAPRSYYHSLSKK
                     ERRRALILALEEKINQGLLRLIEEIDFKSPKTKEAKQFLSSLTGDELRKVLVVTEESK
                     ENVRRAFSNLPDVRVKSVGELTTYFVVDSQLVVMEREAFSSLWRQEDE"
     gene            complement(18307..18921)
                     /gene="rplC"
                     /locus_tag="PKN31_02000"
     CDS             complement(18307..18921)
                     /gene="rplC"
                     /locus_tag="PKN31_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOA98737.1"
                     /translation="MTYGMIGEKVGMTRIFTPEGISVPVTVIKCGPCYVTGKKTKERD
                     GYEAWQIGYGEVKERKLNKPRLGHLKKGGVPPLKFLKEFSFKDTQYQPGEVLKVDGFQ
                     IGDRVDVTGKSKGKGFAGVVKRLGYKGGETSHGSMFHRAPGSIGGTDAGRVFKGKGLP
                     GRMGGKRVTVRNLEVVGINAERDLLILKGAVPGPAGSPVIIIRK"
     gene            complement(18957..19262)
                     /gene="rpsJ"
                     /locus_tag="PKN31_02005"
     CDS             complement(18957..19262)
                     /gene="rpsJ"
                     /locus_tag="PKN31_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOA98738.1"
                     /translation="MAQKIRIKLKAYDHKILDQSALKIVHAVERTGAEVSGPVPLPTE
                     IAKYTVLRSPHVDKKSREQFEMRTHKRLIDIVDPNPKTVDALMHLDLPAGVDIEIKL"
BASE COUNT         5088 a         5170 c         3211 g         5867 t
ORIGIN      
        1 gaggaggctt acttttagtt caaggccaag taccagttcc cttgagcact ccaggcagag
       61 agaaactgag aaaggggata agaattatta gctcctgcaa ttttacccgg gtcgttgaca
      121 tagatattat cagagtcaat gccaactact aacatggcat gttttgctcc cccattgggg
      181 ttcatgttag aataaactgc taccagaaaa ggcttcccct cagaaagcat agctcgagcc
      241 tgctccagat ttcccatccc cactttagtt ggcactcctt cgctatctaa ccaggcacga
      301 acctgaaaaa tattagtcca gtcaccgtta taaccgttga ccggaagtcc aaagtgagca
      361 ttaagccagt tatccgcttg aatgatatgg tcgggggagg gttgaatgtt gaaatagtaa
      421 gctcgcatca tcaccgcaca ggcaggccca caattgcggg tgtttaccca gtcaccaggt
      481 ggaacctggg ggaggaaagg cacgtcaagt acgattctgc tatcagaaaa ctcgataaac
      541 ttatcccgta tctcgctgct ggtgggcgac gaggaaatac caaaattggt ctccggagta
      601 tttatagaat tactgcaccc cgcaacaaga ataaccagga taagcccgaa gatggtaaga
      661 agaaagaaag acttgcgcct catctctcgt acctcctcca gggctttctc cgaaggaatg
      721 tcgagagtga cgcgctaaaa gcttattgct taacgaatct caatcactga gatccggcaa
      781 cccgactgcc ttagcaaggt ctgctttagg ctcgcggctt tgcgccctct cctttcggag
      841 agtttgccct tgaaaatatt aagataaaaa accatactga ccgaatacta ttataatata
      901 tcggcagatt ttttaaaagc tttagggggt ttttagaaag tttttttcga ctcgagttcc
      961 acctcgtttg ggcggaaatt ctcaaaaaat aaggaagtta tccattatgg tctttatttg
     1021 gtagttcctt gctcacttat cttcagagtt atatactctt cttttcctgc cagagtggta
     1081 ctatccagcc ctcatctacc agctccagat tttgataggg attaaaatcc ggggggtagc
     1141 aaacctgtat caaaaataat ccccggggag gaagagccag tcggttaaag gaacgagagg
     1201 gattctccag cataaatttt atctcctcag gatttttctt ccccatgccc accttccaca
     1261 gctctccggc aataattctc accatattat ataaaaatcc atctccttcc acctggagtt
     1321 gaagaagacc agaaggcttc tcctttatct cgatagaata aatagttcga accccggaaa
     1381 aatctgctcc tcccgaggag ctaaaagaag taaaatcatg agtcccttca agaagggaaa
     1441 ggcattcttt cacccgggct aaattaaaat ttttcgggga gaaagaatac acaaaaggtt
     1501 tccaaaaaac tgaaggagga tacataagag aaaaaacata gatgtagatt ttgcgaatag
     1561 ccgtctttcg gggatggaaa gaaagaggag cctccagaac atcaaccact ctaatatcag
     1621 ggggaaggag agaattaaag gcctttttta tggcttccgt ggacatataa gtggaagtgg
     1681 taaaagaagc aacttgccct gcagcatgga ctcccgcatc agttctacct gaagcaaaga
     1741 gggttatttt ctctcttaaa attccttccc ccacctcttc tactactccc tgaatggtct
     1801 ttcctctgct ctggcgctgc cagccttgat aatttgaccc cttatattcc agtaccaaac
     1861 tgaggttgcg gagagacatc agataaaaga gcggagagaa aggagggcaa taaaagaaaa
     1921 agataaaacc agaaaataat aatcagaaga agtgagctcc agaacccgca agcgggtcct
     1981 tccctttcct cctcgataac atcgagattc catggcaata gccagctcat cggctctttt
     2041 gaaagagtta acgaaaagag ggactaaaag gggtattaaa tttttagctc tctgcattat
     2101 acctcccgaa tctaagttca tacctcgagc catttgagct ttcataatcc gatctgcctc
     2161 ttctaaaaga gtgggtataa aacgaagggc aatggtcatc atcatagcta actcatgagc
     2221 cggaaatccc caccttttca gggggctcaa taaatactcc atcccatcgg ttagctctac
     2281 tggtgaagta gtgagagtta aaagagaagt gactaaaatc agaaggcaca gccttgttac
     2341 aattaaaagg cctttcgata ctccctcccg ggaaatatgt aaaaaaccgt attcccagat
     2401 atactctcct ggagtaaaaa agaaatgcaa caccagagta aggataagga gaaaaagaag
     2461 aggcctcaag cttttcaaga gataagtaaa agaaatgtga gaaagagaaa ctaaaaatat
     2521 taacgctatc ccccataata gaaaggaaaa ccaatcttgt aagataaaaa gattaataat
     2581 ggaaatcccc acaaaaacga ttttaacccg ggggtctaag cggtgtatgg gagaatctaa
     2641 tggaacatat tggccaatag ctaaacttct accccacatt aactcttttt ccctccagaa
     2701 ctttaagctg gatttcttct gccagttcct gaggagttaa aactcccgga gtgacatcaa
     2761 aacccctctc tcttaaaagc caggctacct gagcagtcac aggagggaaa atacccaggc
     2821 tttttatcca ctccacttga gagaaaacct gccggatgtc atcatcaaat tttatttttc
     2881 ctttatccat tattattatc ctttttgcta cccgtgctaa atcttccatg ctgtgagaaa
     2941 caattataag agttaagcct ctctctttct gaagtttttc cagttgaccg agtataattc
     3001 tctttccctc ggggtccagg ccagcggtgg gttcatccag aactaaagcc tcgggctcca
     3061 tagagaggat actggcaatg gctactcgtc tcatctgccc cccggagagc tcaaaaggac
     3121 ttttatcttt gacagcctca aaatccagcc ctactgcctc caaagaccat tttactttat
     3181 cttccagctc tttcccctcc acccccaggt tgcgaggggc aaaagctacc tccttaaaaa
     3241 ccgtctcttc aaaaaactga tcttcgggat actgaaaaac cagacccacc ttacggcgaa
     3301 tttcctgcag gggagctcct tttttacccg tatccactcc atccaccagt accctgccac
     3361 tttggggaat caaaagagca ttaaaatgct gaatgagagt ggacttgcca caaccagttc
     3421 tacctacaat accaatactc tctccccgct cgatttccag gtctatatct tccagtgctg
     3481 taacagccag aggtgtaccc ggcagataag taaaagatat attttctaat tttatgaaca
     3541 caactcttcc accagttccc cgggagaaag aggcaaagtc ttaaagagag aaggaaattg
     3601 cttctttacc agaagagcag tttctgttac ctggggaagc tccagccccc actcttctat
     3661 gttttctacc tgagaaaaaa tttttcgcgg tttcccccga gcaacgattt tcccttcgtc
     3721 catgaccagc acttcatctg cttcaatagc ttcttccacg ttatgggtaa tatgtaagac
     3781 agcaatcttc tcccttagac tggcaatcac tttcataagt tcctcccttc cttcggggtc
     3841 taacatagag gtggcttcat ccagtattaa atactccggg tgcatagcca gaaccccggc
     3901 aatagccact cgctgctttt gacctccaga aagaagatga ggttctttat tacggtattc
     3961 ggtcataccc accagagtca gcgcttcttc cactcgagcg agaatttcct cccggggtaa
     4021 acctaaattc tccggaccaa aagctacatc ttcttctact gtagtagcga taatctggtt
     4081 gtcggggttt tggaaaacca gacctacccg acgcctgatt tcccagatag attcttcatc
     4141 gcgggtattc atcccgtcta ctattacctc cccacttttg gggagcagta agccattggt
     4201 atgttttgcc agagtagatt ttcctgaacc attacgaccc agtaaaacca taaacttacc
     4261 ctgatgtatc tctaaatcta cctctctcaa aatctcttca tttcttgctt ctgactcata
     4321 ttcttctcca taccaaaagc ttaccttttt aaactctatc aatttattgg cactccagag
     4381 gcaatgaact actctactat ttccaccaga actaagggag cgccatctcc tcggcgatat
     4441 cccatcttca ccagacgaca gtagcctcct cttctttccc gaaaacgagg cgctacctcc
     4501 cgcaaaagaa tcttaaatgc ttccttatct ctaaggtagg aaatcacttc tcgcaaagaa
     4561 gcattatccc cttttattgc cagagaaacc acccggtcaa aatgtctctt cagtgcttta
     4621 ccttttgctt ctgtagtttg tatcttctcg ttttttatca aagaaactaa aaggttagac
     4681 atcatgctct ttttatgagc agtagtcctt cctaactttt cagtcctccg ccggtgcctc
     4741 acctttattc actccctttt cctctttgct ttctacttcg gaactccact tctctacttt
     4801 ttctactatt tcttcaaaag ttttttgacc cagatttttg atttttttca agtcctcagg
     4861 tcgttcttgg agaatactca ccagttcctg caccgtatcg atatgagctc ttttaagaca
     4921 attgtaagct cttactgaaa gctccagctc ctcaatgctg gtttctgctt cagatgtttc
     4981 ttcatcttct tcgacctctt cagccggttc actttcccct attttttctt tcaataaccc
     5041 cccaaaacgg gagaactctt cagccaaaag atccagagat tggctgagag cctgctgggg
     5101 ggtgatagct ccattagtcc atatttctat aatcagctta tcatagttgg taagctgtcc
     5161 cacccgggta tccagaacct ggaaatttac ttttttcacc ggggtgaaca gagcatcaat
     5221 agggataaag ttataatcct cttttatgtc tcttccttct acctcaagat aaccccgtcc
     5281 cttttttacc gatatcttca tagaaagctt tgcttcctca tctatttctg ctaagtgatg
     5341 gtctaagttg attatttcca cattactgtt aggctgtata tcccgggccg tgacttctcg
     5401 taattctcca ggaactccct ccacttctaa ataaagggtt tcttcatcat cagaataaga
     5461 acgcaaaacc agacccttga ggtttagaac tatgtcgatt atgtcctctc ttaccccggg
     5521 cagagaggaa aattcatgaa ttatcccttc aatttgtact ccggtaaccg ccgcaccttc
     5581 tatagaggaa agtaaaaccc gacgcagagc attacccacc gtaatacccc aacccacctc
     5641 cagaggctca atagagaatt taccatactg aaccttagtg gcgtggtctt cctcccactc
     5701 taaaaggtca caccttgtta tatctcgaat atccaacatt accagcacct caaaattatt
     5761 tagaataaaa ttctactata agctgttctt ccactgcgta atcaatatct tctctttgtg
     5821 gttctcgcaa tatagtagcc ttgccatctt cactcactcc cagccaggca ggagtcgggg
     5881 ttctttcttc ttccattaat tctttaattt ttttatgttt ttccccccga gaagataatt
     5941 ccaccacatc accttcatct accaggtaag acggtatatc caccttgcga ccattaacta
     6001 aaaaatgacc gtgaagaacc atttgccttg catcagcacg agaatctgcc catcccgccc
     6061 gaaaaactac attatctaat cttctctcca aaagagaaag aagattctct cccgtcactc
     6121 caggaaggcg actggctttt tcaaaataat tcttaaactg attttcagtt ataccataca
     6181 taaatcgtac ccgctgtttt tccttaagct gtataccata actggacagc cttcgacgag
     6241 accctttgtg caccccgggc gggaagggtc gtttttccat agcacattta tcagtatagc
     6301 agcgaggccc ttttaaaaaa agcttgctgc catgtcttcg acagagtcgg catttagcat
     6361 cagtgtacct tgacattttc tgtatcctcc ttcataccct tctttgttta ggtggccgac
     6421 aaccattatg aggaatagga gtaacgtctt ttatagagct aatgattaag ccagctgctt
     6481 gcaaagaccg gatagcagtt tctctccccg agccagggcc tttcaccaac acttccactt
     6541 ctcgcaaccc ctgatccatg gctttctttg ctgcctgttc agctgctagc tgagcagcaa
     6601 agggggttcc ctttttcgtc cctttaaatc cagcacttcc agcactagac caggacaaaa
     6661 catttccctc tttatcagtt atggaaacaa cggtattatt aaaagtggac tttatatggg
     6721 caataccttc cctgaccgtc cttttttctc ttttcttttt tctacctgct ttagccaaat
     6781 tagtctaagc ctccctttat catactgttt taattaccta cctctttttg ctcctgcagt
     6841 tcttctggga cctttgcgag tccgggcatt agtacgggtt ctctgtcccc tcaccggtag
     6901 cccttgcctg tgccgcatac cccggtaaca tccaatggaa atcagacgct taatgttttg
     6961 agaaacctga gccctcaact ctccttccac cggatatttc tccacttctt tctggattcg
     7021 cactaattca tcatcactca aatctttgac tctgattcct ggttctacat tagcttcttc
     7081 caaaattttt gccgataact ttctgcctat tccataaatg taagtgagcg caatatctac
     7141 ccttttgtgg ttgggtaaat caactccggc aattctggcc ataagaaaaa aacctcctaa
     7201 ccctgtctct gcttatgtcg gggattggaa cagataacca ttactttccc ccgtcgacgt
     7261 atcactttac acttttcaca tctcggtttc accgaggcac taactctcat cttcttgcct
     7321 cctttttcac ttataccgat acacaattcg ccctttagta gggtcgtatt ttgatatctg
     7381 cacggtaact ttatcaccag gaagaattct aatataatgc atcctcattt tacctgaaat
     7441 atgagccaat attaccttac ccgtttctaa ttctacttta aacatggcat tgggtaaagc
     7501 ctctaaaacc gttcctttta cttcaacaat atcttcttta accattattg ctctttatct
     7561 ccggaatgag ttaaaatcac cggacctttt ttagtaaccc aaacggtatg ctcaaaatga
     7621 gctgacaaac caccatcttt agttaccacc gtccaatcat catccaatat ttctacctgg
     7681 tatcctcctt cattagccat aggctcgata gccaacacca tcccttcttc aataactact
     7741 ccccgatttt tctttccaaa attaggtatt tgaggttcct catgtagggc tctacccacg
     7801 ccatgcccca caaagtccct caccaccgaa aaacccgcag attcaatagt ttcctgaata
     7861 gcatgagata tgtcaccaat tctattaccc ggcaaagctc tttttattcc ctcatataga
     7921 gatttttctg taacttctac cagtttctga gccaaagagt taccatcacc tgctaccacg
     7981 ctgaaagcag catcggtata aaatccctga aactccactc ctatatccac actcactaaa
     8041 tctccagact cgatcacccg ttctccagga ataccatgca ctacttgctc attaatagag
     8101 atacagatgg aagccggata acctcgatag cccaaaaaag ccggtcgagc tccgtggctt
     8161 tttattaaat ctgcagccac ctggtccaga aaagaggtct taactccagg aattatcagt
     8221 ctttttattt cccggataac ctctgctaaa atctttccac tctggcgaat tttttcaact
     8281 tcatcatcac ttattaattt actcattgcc cggctgccac ttctttcaaa gcttccttaa
     8341 ctctatcata cacttcctct ataggagcgc tggatggtat aactcgcaat aaatttcgct
     8401 cttcatagaa atcgattaaa ggtttggttt cttcttcgta agtctccaat cgtttacgaa
     8461 ttacttctgg tttatcatca tctctctgta tcaatttacc tccgcatcgg tcacaaagtt
     8521 cgtctcgctg tggaggatta gaaaggagat tataaggagt ttgacagttt tcacaaaccc
     8581 gccgggaaga aagtctcttt attacttctt ctttatccac tgcaaaatag agaaccacat
     8641 ccaaggataa acccttctcc ttcagcattt cttctaaggc ttctgcctgc tttatggtac
     8701 ggggaaaacc atccaggata aaacctgacc ataaattatc ctggtcaatc ttctctttaa
     8761 ctatacctat taccacatca tcagggacta atctcccact tttaacaaac tcttccgcct
     8821 ttttacccca gggggtttga ttttttatag ccaggcgaat tatatcaccg gtggataggg
     8881 tactaacacc taaatctttt tccatcattt tagcctgtgt ccccttaccc gctcccggag
     8941 gtcccaataa aactatgcgc aatttctctt ctcctttctc atttacggat aaaaccttca
     9001 taatgtctca tgagcaattg agcttccagt tgtctcaccg tctcaatggc cactcctacc
     9061 ataatgagga ttgctgttcc tccaaagtaa aaagtggtaa ccttggtcaa atcggttatg
     9121 atattgggaa ctatagcaat aaaggttaaa aacaaagctc cccacaaagt gatacgagaa
     9181 agagtacggt ctaaatattc taccgttggc cttcccggtc ttattcccgg aataaaacca
     9241 ccatactttt gcatattctc agccagctcc acagggttaa aagaaaccgc ggtataaaaa
     9301 taggtgaaaa atatgatcaa ggctgcataa agagaaagat aaagaggaga attaggagaa
     9361 agagcactgg ctatgctggc cataaccttc gagccctgaa aaaattgagc taaagtggca
     9421 gggaaaagga gaatagaaga ggcaaaaatg ataggaagca ctcctccctg cacaattcta
     9481 ataggtatat gggtgctctg tcctccgtac atcttccttc ccaccactct tttagaatac
     9541 tgaaccggga ttctcctctg cgcttcctga aaagctacta ctacagcaat gattgccaca
     9601 ttcatcacca gagacaaaat gaaatacaaa agattaattt cccccacttt gattaaggaa
     9661 aaagttctca ccaattgggg aagtacccgg gctacgatac cggcaaaaat gataagagaa
     9721 gtcccgttac ccacccctaa atcagttatc aactctccca gccacatcaa aaatatggtc
     9781 ccagcagtaa gagtaagaag aacagttatt ttatatcccc agcccgggta aagaaccact
     9841 cccattcctt ctatccagga ggttattcca aacccttgca ccagagctaa aacaatggca
     9901 aaccacctcg tatactgagc aattttatct cttccctctt ctccgcttcg gcttaattcc
     9961 tctaagctag ggataaccgc cgtcagaagc tggatgataa tggaagcatt aatataggga
    10021 acgatgccca gagctaagat agaaaagtta ctcaaggcac cgccagcgaa catatccaga
    10081 aaacctaaaa ttcctccctg agaaaaaaca tcggctaaag ctcgagaatc aattcccgga
    10141 acaggaatat gagcaccaac gcgaaaaagc accaacataa agagagtaaa gagaattctt
    10201 cttttcaggt ccggtattct aaataatttt aagagggatt cccacatctt atactacctc
    10261 tgctcttccg cctttttctt caatcttctc tttagccgaa agagaaaagg cgtcagcttt
    10321 cactatcaac tttttagtta gctctccccc acctaaaatc ttaactggaa gatccagttt
    10381 cttaatcaac cccgcttttc gtagaagctc tttattcacc tcttctcctt cttcaaaacg
    10441 attaagagct tctaagttga ttatctgcca ttcctttcta ttccagctcc gaaaacctct
    10501 tttaggaatc cgacgcacca gaggcatctg tcccccttca aagtaaggag gaattttgcc
    10561 accagagcga gatttctgtc ctttatgtcc tcgtccacta gtctttccat gacctgaact
    10621 tatgcctcga cccactcttt tccgataatg aaaagaacca tctcggggtt ttaagtcgtt
    10681 aactcccata ttatctttct ccttcctctg cactttctac ttcttctact tctaaaagat
    10741 aagatatctt gttaaccata cccctcaagg aggaagagtc cttatgatac acagtttgat
    10801 ttaatttgcg aagacccaga gctttgacag tccttttatg agactctggt ctgccaatgg
    10861 gacttttctt caaagtaatc ttcagtaatt tctctcccat tttcttctct cctttagcga
    10921 gaaacagtaa agaagtcttt cacttctttc cctcgtaagc gagccacttc ctgagggtct
    10981 ttcaaagaca tcaacccctg catggtagct cgagccagat tcacaggatt attactcccc
    11041 agagatttag tcaacacatc ctttatccct gccacttcta gaactgctct aaccgctcca
    11101 ccggcaataa ctccagttcc cggggcagca ggccttaaaa ccacccggct ggctcctgcc
    11161 cttcccacca attcatgagt gatggtggta cctctcatgg gaaactgaat caaagatttc
    11221 cgagcttttt ccgtggcttt tctcactgcc tcaggaactt ctttagcttt acctgtacca
    11281 atacctacca ctccttctcc gttccccacc gctactaaag cccggaaacc aaaacgcttg
    11341 cctcctttga ccactttgct aactcgattt acttctatca atctttcttt aagctccaag
    11401 ccgtctggct ttattctcct catctttcga cctccatctg gttaaaattg caatccgcct
    11461 tctctggctc cttcagctaa agccttaacc cgaccgtgat atttatatcc tcctctatcg
    11521 aaaaccacgg tttctattcc cttctcccgg gctttctgcg ccagaagctt tcccacctct
    11581 ttagctattt cgactccaga gccatcttta cctctgagct ctgaagtaag ggaagagacc
    11641 gccaccagag ttctgccttc actatcatca atgatctggc catatatatg cctgaggctg
    11701 cggaaaacga caagacgagg acgctcggca ctaccccgta ctttttttcg tacccttcta
    11761 tgtcttttgt ctcttttctc tcttttttcc tctaccagca taataagact ctcccttcac
    11821 ttcgcagttg ctttaccttg ttttcttctg gtaacttcgc ccacatactt aattcctgta
    11881 ccatggtaag gctctacctt tcttagcccc ctgatattag ctgctacctg acctactttt
    11941 tctttgtcaa taccttttac tttcactacc tgtccctcca cctcaaaggt aatgccggag
    12001 ggaggttcta tctctatggg atgggaaaat cccaggttaa gaactaaatt ttgtcctttt
    12061 ttctgagctc gatagcctaa tccgctaatc tccagagctt tttcaaaacc ctgggacact
    12121 ccttccacca tattagccac cagagtccgg gttaagccat gaagagaacg atctaattta
    12181 tcttctcctt ttctaattac ctgaagagta tttccttctt tctccacttt cactcgagga
    12241 tgaatttccc tctctaaggt tcccagagga cctttagcca ctatcgtgcg accctcaatt
    12301 tttacattta caccatctgg tatcactacc ggtttctttc ctacgcggga catggttaat
    12361 cctccttcac caaatatagc aaataacttc cccacctacg cctaattttt tagcttcttt
    12421 tcccgtcatc atcccttgag aggtggaaac taccactgtt cccattcccc ccagaagcac
    12481 aggtatttca ttttttccgg catacactct caaacctggt ttacttattc ttttcaaacc
    12541 attaattact ctttctcgat taggaccata tttcaactcg ataatcaagt cactctttcc
    12601 accttcctga gtgagaactt tataatcgtt aatgtagcct tcctctctaa ggattcgagt
    12661 tatttcaatt ttagaccggg aggaaggaac tttaactata ctctctcccg cttttaaggc
    12721 gttcctaatc ctcgtcaaca tatcagcaat gggatctgta tgggccacta tgtttttcct
    12781 cctttaccag ctcgacttgg ttaccccagg tatttcacct ctgcttgcca aattacgaaa
    12841 acaaatccta cacagaccaa aatcccgcaa gtaaccccga ggtcttccac ataaactgca
    12901 tctatttcta tagcgcacct taaacttcaa atcctttttt tctctttcta ttttagcttt
    12961 acgagccatt aaaaaacctc ctctacttcc taaatggtaa acccagcgct tccagcaaag
    13021 ctttagcttc ttcatcagtg gacgcagtgg ttacaaaagt gatattcata cctctaaccc
    13081 gctccaccct atcatagcta atttcgggga aaattaattg ttcttcaatg ccaatggtac
    13141 agtttcctct cccatcaaaa gaactatccg gaaatcccct gaaatcacga atacgagcca
    13201 cagctatatt caggaaccga tccataaatt catacattgt attttttctc aaggtaacct
    13261 tacaaccaat tggtgcgcct cggcgcaact taaagttaga tatagatttt ttagctcgag
    13321 tcaccaatgg tttctgcccg gtaataagag ccaactcttc tattgctaaa tcaaggtagc
    13381 gggagttttg agtagcatcc cctaccccca cattaataac tattttttct accttgggca
    13441 cttgcataac atttcgataa ccaaaagtct caacaagact gggaattact tctttctggt
    13501 atttttcctt ggtgatggac acagccacaa tcctccttgc tacaccttat ctatgatttc
    13561 tccacatttt ttacatactc tgactcgaga acgggactca gccactttaa ctctgcgaat
    13621 tttagtaggt tgattacagc gactacaaat cagtcttaaa ttagaaattt ttaccggatt
    13681 ttctctgttt attattcctc cctgagggtt gtcctgagtg ggacgggtat gttttttaac
    13741 tatgttaatt ccctctacca ccacttttcc ttcccgggga aaaaccttca gaactttccc
    13801 tctttttccc cgattcttac cccggacaat ctctactgaa tcaccttttt ttatggctac
    13861 actagctttt ttctctcgac tttccatttt aaccaccttc ctcataccac ctcaggagcc
    13921 agtgagataa tacgcgaaaa attacggctt ctcagctctc ttcccaccgg accaaaaatt
    13981 cgagttcctc gaggcacatc ctgattggtg accaaaacag cagcattatc atcaaaacgg
    14041 acaaagctac catctggtct ccctacttcc ttcttggttc tcacaaccac cgctcttacc
    14101 acttcccctt tttttacatc agagcgaggt tctgcttctt ttactgtagc aataattacg
    14161 tcaccaatac ttgcataacg gcgaccagag ccacccagta ctcgaatgca cattattttt
    14221 ttagctcccg tattgtcagc tacctcaagc atagttctcg actgaatcaa cttcttctcc
    14281 tccctctacc ctggcttttt gcaacaccgt aacaactctc catcttttag tcttgctcaa
    14341 gggccttgtt tcctcaatca gaacccgatc ccccactgca caaattccat tctcgtcatg
    14401 agcagagaac cgactcgctt ttctaatttt ctttctataa agagggtgtt cagaaattct
    14461 caccactttt accactactg ttttatccat agcattacta actacttcgc cagttaatct
    14521 ttttcttgct cccattctac ttaaccccct gcttctcttc agacaattct ctttctctca
    14581 aaatcgtctt cactcgagca atgtcttttt taaccatttt tattctctta ggattaccca
    14641 attgaccggt aatgctttga aaacgcaaat taaaaagttc agtacgtaaa tctttgaatc
    14701 tctgatttag ctcttcatta gtcaaattgc gaagctctaa agccttcata tctctcaccc
    14761 ctaatcaatg cttctctcca ccaccctggt cttgatcggc aatttgtggg aagccagtcg
    14821 gagagcttcc tgtgccgttt ctctatctac tccccctatc tcaaaaagaa tctttcccgg
    14881 ttttaccact gctacccatc cttctaccgg cccttttccc ttacccattc ttgtttctgt
    14941 gggcttttta gtatagggct tatcaggaaa gacacgaatc catatttttc cttttttcat
    15001 actacgagat agagccactc gagccgcctc aatctgagga ttagtaatcc aggctggctc
    15061 cagagcctga agaccgaaat ctccaaaatc aactttatta ccccgggagg ccacaccctt
    15121 cattcttcct cggtgttgtt ttctatattt aactttcttg ggcatcaaca tagtcttctg
    15181 ctacctcctc ataacttcct tcttccttct cttttttctc ttttcccgaa agagcagcac
    15241 cttctgcctc taacaactct ccaggagaaa tcacttctcc cttgaatatc cacactttta
    15301 ctccaatttt tccataagta gtgagagatt cagtaaagcc ataatctatg tctgcccgca
    15361 aggtatggag aggaagacga ccttctcgat accactcttc ccgcgcgatt tctgctccgg
    15421 ctaaacgacc ggaacaggat atttttatac cctgagcacc catccttaaa gctctattga
    15481 tagcctgttt catagctcgc ctataagaaa ctctccgctc tatctgggaa gcaacgttct
    15541 cggccaccag ttgagcatca atctccggga tgggcacctc ttccaccgaa atctggatgt
    15601 tctgatttcc ggtcatattc tggatatcct gacgaagctt ttccacctcg ctgcctcttc
    15661 tgccaatgac aatgccaggc cgggaggttt taacaattat ctttatctgg ttagccagtc
    15721 gctccacttc cacccgagaa atacctgaac gataaaatct atttttaact tcctggcgaa
    15781 tcttcaaatc ctctataaga taatctttaa acttattttt ttctgcgtac cagcgagatt
    15841 gccaatcctt aacaattccc aaacgcaacc caattggatt tactttctgc cccaattatt
    15901 gttcctcctt ttccgaaact gccactgtaa tatgagaggt tcttttcctg attagatacc
    15961 ctctccccat agcccgggga cgtacccgtt ttaaagtcgg cccctgatct acaaaagctt
    16021 tagacactac taattgatcc acatccagat tatagttatt ctctgcattg gcaaccgctg
    16081 acttcaaaac cttatacacc atttcggctg actttttagg aataaactgt aaaatagtta
    16141 aagcttcctg cgccgatttt ccccgaatta aatcaacaat ttgccttgct ttgcgaggcg
    16201 agatgcgcaa atattttgcc gttgctttta cttccatatt ttccaatcct ccctcacctc
    16261 aacgccgtag agcgttccgt gggactacca tgtcctttaa aagttcgagt aggagcaaat
    16321 tctcctaact tatgccctac catttgctca gtgatataaa caggaatgtg ctttttcccg
    16381 ttgtgaacag caatagtatg ccctaccatt tcagggatta tcacacaagc tcgacaccag
    16441 gttttaatta cccttttctc tccctttcga ttaagttctt caattctttc tcgcaagcgt
    16501 gaatctacat aaggaccctt ctttgtagat ctacccatcc ctacttcctc ctcttaacaa
    16561 tccatttatc cgtctttttg ttcttcctgg ttttaaagcc tttagtaggc actccccagg
    16621 ggctcaccgg atgtcttcct ccgtgggatt tcccttctcc tccaccatga ggatggtcga
    16681 taggattcat agctactcct cgcacggtag gcctcactcc taaccatcgt ttccttcctg
    16741 ctttgccaat ggccacattc tcatgttcta cattgcctac ctgacctacg gtggccaaac
    16801 aatctatatt aatcaagcgt acttccccgg aggaaagtcg aacttgagca taatctcctt
    16861 ctttagccat taactgggca taagcccctg ctgatcttgc taactgtcca cctttgccct
    16921 ttcttaactc cacattatga atgaaagtcc ccagcggtat attacgaaga ggcaaagcat
    16981 taccaggacg gatgtccgcc tcttctccgc tcattaatac atcccccacc gtgactccta
    17041 aaggagcaat gatgtaacgc ttttctccat cagcatattt caaaagagcg attcgagcag
    17101 accggttggg gtcatactca attctttcta ctttagcagg aatgccaaat ttatctcttt
    17161 tgaaatcaac acaccgatat tttctcttgt ggcctcctcc tcgatgtctt accgaaattc
    17221 tcccctggtt atctctcccc gctttacttt tcaacggttt taaaagagat ttttccggcc
    17281 caccaggagt aatttcttca aaggtatgac cactcatatg tcttcggcct ggtgtaactg
    17341 gcttgtattg tttcaaatta gccatattgt tctctccttt ttaagtaata tcaaaggctt
    17401 ttattctttc tcctggcttc aagaaaacta tcgctttctt atatgagcta gttttaccct
    17461 caaagcgtcc cagccttttt gttttccctt taaccctcac cgtgtttact cgagttactg
    17521 tcaccccaaa ggcatcttcc actgctttct ttatctctgc cttagtggct cgagaatcaa
    17581 ccttaaaaac atatttactc tccttctgga ggttcactgt cttctcggta atgatagggt
    17641 taatcaatat ctctcgctga ttactcatct tcctgcctcc aaagtgacga gaaggcctct
    17701 ctctccatca ccaccagctg ggaatccacc acaaagtaag tagttaactc accaactgat
    17761 ttcactctaa cgtcagggag attggaaaaa gctcttctca cattttcttt actttcctca
    17821 gtaaccacca acacttttct tagctcatcc ccagttagag aagaaaggaa ttgcttggct
    17881 tctttagttt tagggctctt aaaatcgatc tcctctatca aacgcaacaa tccttgattt
    17941 attttttctt ctaaagctaa aatcagagct ctccttcttt ctttcttaga aaggctatgg
    18001 tagtaagagc gaggagcagg accaaacgtt acccctcccc ccttccagat gggagagcgg
    18061 atacttccat gtcttgctcg accagttcct ttctgtctcc agggttttct ccctccccct
    18121 cttacttccc cccgggtttt agttttggcc gttcctaatc tttgattatc taaataagcc
    18181 ttgaccgaga ggtaaagaag gtgataatct tcatcggaca gaactgtatc ttctaaggtg
    18241 atggtgtcta ccacctttcc ctctttgttc cacacttcta actccatctt ctatccccct
    18301 atctggttac tttcgaatta ttattactgg acttcctgcc gggccaggaa ctgctccttt
    18361 taaaatgaga aggtctcttt ctgcatttat tcccactact tccagattgc gaactgttac
    18421 cctcttccct cccattctcc cgggaagacc ttttccttta aaaaccctgc cggcgtctgt
    18481 accacctata gagccaggtg ctctatgaaa catagaacca tgagaggttt ctcctccctt
    18541 gtatcccagt ctttttacca ctccggcaaa ccctttaccc ttggacttac cggttacgtc
    18601 cacccggtct cctatttgaa agccatctac tttcaatact tcacccggct gatattgagt
    18661 atccttaaaa gaaaactctt tcaaaaactt aagaggaggc actcctcctt ttttaaggtg
    18721 tcccagacga ggtttattga gttttctttc cttaacctct ccataaccta tttgccaggc
    18781 ttcataccca tcccgctctt tagtcttttt gccggtaaca taacagggac cacatttaat
    18841 taccgttacc ggaacagata ttccttccgg ggtaaaaatc ctggtcattc ctactttttc
    18901 tcctatcatt ccataagtca ctgttttcat ctatatctcc ttccccagct acctcatcaa
    18961 agcttgatct ctatatctac cccagctggt aaatcgagat gcatcagagc gtcaaccgtc
    19021 ttagggttgg ggtctactat atcgatcaat cttttgtggg ttcgcatttc aaattgttct
    19081 cgagattttt tatccacatg aggagatcgt aaaacagtat acttagcaat ctcagtgggt
    19141 aaaggcacag gccccgagac ttctgccccc gttctctcca ctgcgtgcac tattttgagt
    19201 gccgattgat ccagaatttt atgatcgtaa gcttttaact ttattcttat tttttgagcc
    19261 acttcctacg cctccctctc cccctattca ataatatcag ctactactcc tgctcctact
    19321 gttcttcctc cctcac
//
LOCUS       DAOPSS010000016        18983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2457_length_18983_cov_8.810915, whole genome shotgun sequence.
ACCESSION   DAOPSS010000016 DAOPSS010000000
VERSION     DAOPSS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 18983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18983
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2457_length_18983_cov_8.810915"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..966
                     /locus_tag="PKN31_02010"
     CDS             31..966
                     /locus_tag="PKN31_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA98739.1"
                     /translation="MREEGSNLLIIKSDISLLLVNLIWGATFVTMKNLLESVPPLAIL
                     FWRFAIATFLLLLISLFSKWHKIDWVSGVSLGMCLFAGYLFQTWGLIYTTPARSAFIT
                     GLSVILVPPLAFLLLKSSIDQFSIVGTIISLFGLGLLAWQKGETKSIFLLGDFLTLLG
                     AAAYALQIVLVEKFTKENESLPLATIEMGTVALLSLVFGILFHQNMNPCFSGREWGSI
                     LFLGIFATALAFTLQKRAQKHTSSIHAGLIFASEPVFAAIFSYFLWQEKFTPRTIAGC
                     ALILGGILFSQLGLLSNQSTDSMSSIFKSNSKPST"
     gene            complement(909..1061)
                     /locus_tag="PKN31_02015"
     CDS             complement(909..1061)
                     /locus_tag="PKN31_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98740.1"
                     /translation="MMRIVLSNFRRKGDVLNRAEEQIVLQKIIDLLYVEGLEFDLKIE
                     DIESVD"
     gene            complement(1058..2284)
                     /locus_tag="PKN31_02020"
     CDS             complement(1058..2284)
                     /locus_tag="PKN31_02020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOA98741.1"
                     /translation="MKVAERVKKASQLKDWVADRVFRLKGEGAFEVLAKAQKLEKQGR
                     EIIHLEIGEPDFDTPINVKEKAIQAIQDGSTHYVPSAGIDELREAVCVYLRKSRGIEV
                     FPEEVVITPGAKPVIFLSFLLLLEEGDEVIYPDPGFPAYSSIIEFIGAKAVPLRLREE
                     NGFRVDVDELRSLITSKTKMIVINSPHNPTGSVLSEEDIEAIARLAQEEDLLVFTDEV
                     YSEIIYDQKHVSILQFPGMKERTVMLDAFSKSFAMTGWRLGYAVLPEEWARFLSLLVT
                     NSNSCTCTFTQIAGVEALLNGEESVQVMAQEFRRRRDFMEKRIAQIEGIKIIKPQGAF
                     YFFPNITSFGLSSKELADYLLKEGGVAVLPGTSFGHYGEGYLRISFANSLENLARAMD
                     KMEEALNKLTKRGGRK"
     gene            complement(2392..3006)
                     /locus_tag="PKN31_02025"
     CDS             complement(2392..3006)
                     /locus_tag="PKN31_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOA98742.1"
                     /translation="MNKREKILEGARKVFAEKSFFEATLEDISLLSGIKKSTIYYYFD
                     SKLELLLEIVDEIIQRAIESIADIPFSLSPRETLERVIEGYFTFFHQERDSILIFRRV
                     GYDFFAHPEALQELRQSFQKFREIRESLGKRIGVLKTQRGNEIDGTEIIRIILSSITA
                     YWIEEIVEGREIKLEDKEIFKDIFTSFIKEGNNGKAKRLSVAEP"
     gene            complement(3238..4533)
                     /locus_tag="PKN31_02030"
     CDS             complement(3238..4533)
                     /locus_tag="PKN31_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98743.1"
                     /translation="MDGKRILILASFGLLLFALPLVAGEASEITGEAKIGASYDLEKG
                     ESAWGGSLNLKYDTSISSNYYFTADLLLSYQSEGYLQPVMINELFLQGIETPSEEVDF
                     RLGLLQLSWGASDIMSPVDVINPTPFSLSFSEDALKNKIPVPALDLEWYHSWDWSLEF
                     FYQPDFVPNFVPEGLEKQILAYELASLLSLPPQNLSLSLTKEEPLVDFSHPIWGVRAR
                     GIVSSVDVAVSYLQGYYLSPYPRETNVFLGLDGSSVVDVTMGYPRRSMLGLEFQGEFP
                     LLEGSTFRGDIALFVPERWINRINITQPSGEVISYPQEVFQNPYWKGSLGIDYTTDNN
                     TLLNLVWMWGNPYEEGEDISPYLFLAAERPSDDNKWTALLTSGVSLQDGSMVNVVGLT
                     YKPEDDWNISLSYSFSSGAPGSKLAGVGDGVFLGVKYTF"
     gene            complement(4545..5309)
                     /locus_tag="PKN31_02035"
     CDS             complement(4545..5309)
                     /locus_tag="PKN31_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOA98744.1"
                     /translation="MKKILSLMVWGLVILIGVVPAWAITADEILDEVEARTAESETQK
                     AIAKMILVDSKGREEVREVIMYAKDEGEDVISIIVRFLSPADVKNVTLLSLEGGESMY
                     LYMPAYKKVRRIASSEKSEKFMGTDFTYDELGGSYSREDYDSTLIEETEEIYKLELIP
                     RDEESSYSKLLMEVDKEKFYFRRIEFYDKDGNLWKVMEILEIEEEEDGTITVNKIDLT
                     DLKENHKTIVEMEHIERDIPLPDNFFSVRTIQRPEL"
     gene            complement(5315..8107)
                     /locus_tag="PKN31_02040"
     CDS             complement(5315..8107)
                     /locus_tag="PKN31_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOA98745.1"
                     /translation="MQKISQWIVKNAWLILIAVLVVTIFFGFQVVNVKFKDDVTEYLP
                     EDDPEISFYNSLSDKFASFQKKSMIVALEFDDLFTPHNMELLQEIVESIEELSSVRSI
                     SALTNMPKVIATDFGIEVREVVEVLPQTPEEAEELRRDLEEDKLIWGKMVTEDGKGTI
                     IAVSFYEAVDEEEAVDEVRAVCRPYEEKVQITYFGLPIIIEQTSYDAQRTLRVLAPLS
                     GLLLLLILYWGFRSLQGVILPVFVALLASLWTLGLAALVGESLTVVSATLPVILLAMV
                     TAYGIHFVNRYYEERSRMGGDDVVKVTLQEIFIPILLSALTTMGGFISLLSADIKPIT
                     EFGLYSTLGIFFGFLLSTFFLGAFFTVFVPRRLPRYYSQEEEEDSQDLIGRFLLFMAR
                     GITHHRNLIIAIIVVALAVLTIGIPRIQVETTVEEQIGVNHPLTKLLKYFQERFGGTS
                     YNYLYIETEKVKEPFVLREMIRISRFAERYETFADPSSIADFICNLNEAMDGWKAIPD
                     TREKVENLWFFAQDNSFIQDRISEDEDATLVEFKARETTSRQLEEEVEALKSFLAQRP
                     QEVKVVSVTEEGAAEYLATSIVEDLEIFGLSFSDPEAVEQMVFQFIGQPLRDFMAFDE
                     EFAKKVAHDAFLEIEGLGLEEEEVEEALLSYFSHPHFSLSSVLETDLGITEDDALYLA
                     EVLDLSMERVARNNKASALRQEIENLVGQTLDSDFDFVFYQVLDEYVFLPQENGDILV
                     YYQITGTPIIANHINQQLFTRQIESLILAFVIIYVLLLLQLRSAKKSLVAIIPILMAV
                     YTSFGLMGLLGIPLNTATLMVASIAIGAGIDYTIHLTSRWYMEVEKGSSNPLRTTLVH
                     TGRGIVLNSLAVSGGIYILTLSRIRMLRMFGDLVATVLLVTMFYTLMVLPLLLHAAEY
                     GNNKNNNKKNKDMERG"
     gene            complement(8442..9800)
                     /gene="mgtE"
                     /locus_tag="PKN31_02045"
     CDS             complement(8442..9800)
                     /gene="mgtE"
                     /locus_tag="PKN31_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOA98746.1"
                     /translation="MGERNIVSFKESAEEMLASQDYEALRELLEELHPYEVATLLEEL
                     EEPEQLIILELMPTDMAAETLEHLEYEDQYRLLDHLEDTVARKIIAAMPSDVIADLVM
                     AIHPHQAQKILGMVPEEYLQKIEYLMTYPEDSAGGIMSLEYIAVRERWTVERVIEHFR
                     KVGRKESIASYIYVVDNIGQLVGVVSLKELLLSHPKTPISEIMHTNVVAIPVDADQEE
                     AAKLMSQYDLLVLPVVDSRGRLVGVITADDIMDVIEEESTEDIHKLGGSKPLDLPYMH
                     SSLITLFSKRINWLIFLFAVQAVTSNILKHFQSVLSSVVALSFFIPLFTDTGGNSGTQ
                     ASSLIIRSLALGEVTTKDATRILIKELQVSAMLGATMGIVGYFFAWAVAGSVEIAIAV
                     AITLFVVLVVSSTIGAMLPIIGSKLGIDPAVFSAPLITTVVDIVALTLYFNIATLILG
                     IK"
     gene            complement(10412..11266)
                     /locus_tag="PKN31_02050"
     CDS             complement(10412..11266)
                     /locus_tag="PKN31_02050"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOA98747.1"
                     /translation="MITAQTRLLGVIGYPIEHSLSPLFQNAALSYLGLPFVYLAFDIL
                     PSNLERALEAMRIFSIRGFNVTIPYKEKVIPFLDALEGEARVIEAVNTIVNEKGKLIG
                     YNTDGVGLYRSLERKGVNVEGKKALLLGAGGAAKAAVWALWKRGVKSIFVINRTRERG
                     EDLANWARAEFFLEVEVVEWGKMKEQDSLPLWQEAGVIVNATSLGLKGEEISLPWEKL
                     SPSVWIVDVVYRRGLTPLVREAKKRGIQSFDGKEMLLQQGAESFFLFTGEKAPLKVME
                     EALDWKPL"
     gene            complement(11263..11760)
                     /locus_tag="PKN31_02055"
     CDS             complement(11263..11760)
                     /locus_tag="PKN31_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOA98748.1"
                     /translation="MSWWKKLTPDLMTESLSDISLDFLGKKGIRGLIIDLDNTLVPWG
                     GFEFPPGVKAWIQEAQERGFKIFLVSNALEERVNHFRESLDIPGISQAQKPRRGAFRL
                     ALAQMGLEKNQVAVIGDQLFTDVWGGNRLGLFTILVSPVNEKELFFTRVVRRLERRVL
                     KRLKA"
     gene            complement(11757..12731)
                     /gene="mltG"
                     /locus_tag="PKN31_02060"
     CDS             complement(11757..12731)
                     /gene="mltG"
                     /locus_tag="PKN31_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOA98749.1"
                     /translation="MRSKFILYLSLSWLLCLFLALAFYYGGEEGERKIVVIENGLSGR
                     EISYILEEEGIVPSFLFRLFLFLSNQDKLIAGNYFLYPGEAVGEVVKKLTQGPSYEKI
                     TFPEGFTSEQMARVLEEKGICSARDYLDLINKPELFSAGWLGGITNLEGFLFPDTYYF
                     SPFTSPQKVIETQLHHFEEVFLPHYWLSRSSLSLKEVVILASMVEKEAKRKEEKPVVA
                     SVFINRLKQGMKLQSCATVVYALYREKGLKVENLKEEDLKVASPFNTYLISGLPPQAI
                     CNPGLDSLLSVLYPQKTDYLYFVLQGDGKHAFSQTYEEHLHYKEGNNP"
     gene            complement(12721..13182)
                     /gene="ruvX"
                     /locus_tag="PKN31_02065"
     CDS             complement(12721..13182)
                     /gene="ruvX"
                     /locus_tag="PKN31_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOA98750.1"
                     /translation="MGRANFSFRRLGREFMKRVMAIDWGEKRIGVAFNQGTGFSFPCE
                     VIEAEGEEAINKILALAKELKAEVIVVGLPLHLDASFQEEARRVEKFKKKLEQSFEGE
                     VVLWDERLSTKEAEKRLIEADLSRRKRKKVIDKVSAALILETYLSHMGNEV"
     gene            complement(13134..15752)
                     /gene="alaS"
                     /locus_tag="PKN31_02070"
     CDS             complement(13134..15752)
                     /gene="alaS"
                     /locus_tag="PKN31_02070"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOA98751.1"
                     /translation="MTSAEIRKAFLQFFEEKEHKILPSASLIPEDPTILLTIAGMVPF
                     KSIFEGRVSPPFHRAVTVQKCVRISDLDKVGYTSRHHTFFEMLGNFSFGDYFKKEACS
                     WAWEFLTQRLLLPPDKLWISVHHRDEEAFTVWREEVGISEEKIVFLGDEDNFWASGPV
                     GPCGYCSEIYYDTGEERGCGQPDCRPGCDCDRYLEIWNLVFMEFDRGNDGLLRELPHK
                     NIDTGMGLERITSVMQKAQTDFETDLFLPIIEKIEKMSGISYQREDLRPYFRIIADHI
                     RAITFLIGDGVYPANEGRGYVLRRLIRRAHRYGHKLSLYHPFLPELAFVVIEIMGHHY
                     PELKDRKKLIAQVVLQEERKFEETLQLGMGYLKDLISRFQEKEERVIPGREIFYLYDT
                     YGFPPDIASEILEEEGLSFSQEEFETEMERQKTRARRAYEEKTQEFKERKDEEEIFSG
                     LESEFVGYEEKEIETQVLALFSQGERVKEVKKGEEVIVVLEKTPFYPERGGQEGDKGF
                     ITSSQGEIRVEQVSAPSSSLIIHRGVVEEGIVREGEKARAQIEARRRKAMEIHHTVTH
                     LLHRALREVLGEQVKQTGSWVGEEGLRFDFTHFAPLSSEEIKAVESLVNEKIFEDLPV
                     QITFSTLEEAQKRGVIALFEEKYEEMVRIVRVGNYSAELCGGTHLAHTAQALLFKITS
                     ESSIGRGLRRIEGVAGPSALSYLNQKEQILQDVVKTVNVEEGKVKEYIEKVVGENKEL
                     KKALEKERRRRIEEKIKSQISSVSNEETTLVSYLGEEDGLDLDFLKEVVDHLRPFIQK
                     GVVVLGLKKEGKIQGVLAGIGLPPEANLGRMIKEWSQKIGGGGGGRPHLAQFGGLSEE
                     KWEELISLLEDWVESL"
     gene            complement(15709..16740)
                     /locus_tag="PKN31_02075"
     CDS             complement(15709..16740)
                     /locus_tag="PKN31_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family protein"
                     /protein_id="HOA98752.1"
                     /translation="MTDRTNPSLTRAQDVIQELKEVSSSFERVVVAFSGGMDSTLALF
                     LSLQALGKEKVISCTVDWDIYFPSLARESVDYWVDNLGVDHVYLPGRKVMEEIMKTGP
                     ACNRCTKEAKLGTIRRYFGNRVLIVGGANQSDSWGKRGVKLLNNTYSPLFELSKEEIV
                     NLASFLSLPLRRMGENKLREGCLLKHLLKPLASPYQAQAVVKSNEYLLKILNERNIER
                     DIANVKIIGPLNRNIALVNVKPLPSLALREEITAVLSSIEEIDEVSWVDSPITLVVRA
                     NLGQYRNLSSLYWLEKGKLQPEFAFPITVRWMPSSNRRLHTFQVVDFKKENTNYDQCR
                     NQESLSSVF"
     gene            complement(16737..18668)
                     /locus_tag="PKN31_02080"
     CDS             complement(16737..18668)
                     /locus_tag="PKN31_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOA98753.1"
                     /translation="MENGERMELTHEDLGEIRIVRPGDLVKGKVIKKGEENYFVNINS
                     KSEGLLPLREKMVGKEGEVENQLEEDDETWVVVTHIDEQGYVWLSREQARYQGAWIDI
                     EESQNENKTLTAKVKKRVKGGLIVDVGINAFLPASCVDITPKNLDEFIDQTIPVKVIE
                     ADRKTKNVVVSRKAVLEEERERQKKELFDSLEVGQVRKGIVKNITKFGVFIDLGGVDG
                     LLHVSEVSWKKTFGNLEDLFQKGEEIEVKVIAFDPEKEEISLSLKRLQPDPWEDIDEK
                     IRPGDIVKGKVVSVRDFGVFVEIEEGLEGLIHISDLSWGYVKHPQEVVKVGSIVEARV
                     LEIDKNKRRVSLGIKQVLPDPWEEVDEKYPLGSTVRLRVSSITPRGVWGEIEEGVEGR
                     IPLEELSWKRVNRVSEIVRRNQVVEAKILEVDTQNRQILLSIRQLRPNPWEEIAGKWK
                     VGDMVEAKVRRLMNFGAFVELVPDVEALLPLSELDWEPVKHPNQILKRGQTLSLKIIE
                     FNPEEQRIVVSRKATLPDPWEEIKNSYPVGSVHLKKIVRLVDFGAFIELEKGWDGLVH
                     ISEISNQRISSPSEVLKEGEEVKVKVIKLDDQERKIGLSIKQASQEEEKDYASSNGKI
                     TLGDMVGGKLTDFLNSLKK"
     gene            complement(18705..18861)
                     /gene="ssrS"
                     /locus_tag="PKN31_02085"
     ncRNA           complement(18705..18861)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKN31_02085"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT         5655 a         4611 c         3143 g         5574 t
ORIGIN      
        1 tccattgata agtagtatat actattattc atgagagaag aaggttctaa tctactaatc
       61 atcaagtctg atatctctct ccttttagtc aatctgatat ggggagctac ctttgtcacc
      121 atgaagaatc tcctagagag cgttcctcct ctcgctattc tcttctggag atttgctatc
      181 gccactttct tacttcttct aatttctctt ttctccaaat ggcataaaat tgattgggtt
      241 agcggtgttt ccttaggaat gtgtcttttt gccggttatt tatttcaaac ctggggattg
      301 atatacacca ctccagctcg ctctgctttt ataactggcc taagtgtgat actcgtaccc
      361 cctttggcct ttttacttct taaatcctca attgaccagt tttcgatagt gggaaccata
      421 atatctctct ttggactggg gcttttagcc tggcaaaaag gagaaacaaa atctattttt
      481 ctcctcggtg atttcctcac cctcctagga gcagctgcct atgctttaca gatagtatta
      541 gtagaaaagt tcactaaaga aaatgaaagc ttaccccttg ccaccataga gatgggaacg
      601 gtagcccttt tgagtttagt gtttggaatt ctattccacc aaaacatgaa tccctgcttt
      661 tctgggagag aatggggaag cattttattt ttaggaatat ttgccacagc tttagctttt
      721 accttacaaa agagagctca aaaacacacc tcttccatac acgctggatt aatatttgct
      781 tctgagccgg tatttgctgc tatcttttct tattttctct ggcaggaaaa attcacaccc
      841 cggactatag cggggtgtgc ccttatattg gggggaatcc tattttctca actgggttta
      901 ttatctaatc aatcaaccga ttctatgtct tcaatcttca aatcaaactc taaaccttct
      961 acataaagaa gatctataat tttttgcaaa acaatctgtt cttctgctcg attcaaaaca
     1021 tctccctttc tcctgaagtt agataggaca atcctcatca ttttcttcct cctcttttcg
     1081 taagtttatt aagtgcctct tccattttat ccatagcacg agccaaattt tctagagagt
     1141 tagcaaatga tattctcagg taaccttctc cataatgacc aaaagaagtt ccgggtaaaa
     1201 ccgctactcc accctctttt aaaagataat cagccagctc cttagagctc aaaccaaaag
     1261 aagtaatatt aggaaagaaa taaaaagctc cctgaggttt aattattttt atcccttcaa
     1321 tctgggctat tctcttctcc ataaaatctc tccgtcgacg aaactcttga gccataacct
     1381 gcaccgattc ttccccattc aaaagagctt ctaccccggc tatctgagta aaagtacagg
     1441 tgcaagaatt agaattagta actaaaagag agagaaacct tgcccactcc tcaggtaaaa
     1501 cagcgtatcc caatctccaa ccagtcatag caaaactttt agaaaaagca tccagcatta
     1561 cggttctttc tttcataccc ggaaattgca aaatgctcac gtgtttttga tcataaataa
     1621 tttcactata gacctcatcg gtaaacacca gtaaatcttc ttcctgagct aatcgggcaa
     1681 ttgcttctat atcctcctcg ctcagaactg aacctgtggg gttatgggga gagttaatca
     1741 ctatcatctt agttttgctg gtaataaggc tcctcagctc atctacgtcc actctaaatc
     1801 cattctcttc tctgagacgc agcggtactg ccttcgcgcc tataaactca ataatagaag
     1861 agtaggcagg gaaaccagga tcgggataaa taacttcatc cccttcttcc aataaaagaa
     1921 gaaaactcag gaaaataact ggtttagccc ccggagttat cactacttct tcagggaaaa
     1981 cttcaatacc tcgacttttt cgtaaataaa cacatactgc ttctctcagc tcatcaattc
     2041 ctgcagaagg aacataatgg gttgacccat cctgaatagc ctgtatagct ttctccttga
     2101 catttatggg tgtatcgaaa tctggttctc ctatctccag atgtattatt tccctacctt
     2161 gtttttccag cttctgtgcc ttcgccagaa cctcaaaggc tccttctcct tttaatctaa
     2221 aaaccctatc tgctacccag tctttaagtt gagaggcttt tttgactcgc tctgctacct
     2281 tcatcgcttt cacctacctt attgagcaat actttaacgg gattaaggca ttttggcaat
     2341 accctgacaa aaaattcttt gagtttttag taaaactaac tgtaaaatct tttaaggctc
     2401 agctaccgac agcctctttg ccttcccatt attaccttct tttataaaag aagtaaaaat
     2461 atctttaaaa atttctttat cttctaattt tatttctctt ccttcgacta tctcttctat
     2521 ccagtaagca gtaatgctag ataaaattat gcgaataatt tctgtaccat ctatttcgtt
     2581 tcctctttgg gtttttaaga caccaattcg ttttcccaag ctttctctta tttctcgaaa
     2641 tttttgaaaa gattggcgaa gctcttgaag tgcttcagga tgggcaaaaa aatcatagcc
     2701 cacccgccga aaaattaaaa tagagtctct ttcctggtga aaaaaagtaa aatacccctc
     2761 gataactctc tccaaagttt cccgaggact caaagaaaaa ggaatatcgg cgatgctctc
     2821 tattgcacgc tgaataattt catcgacaat ttccaacagc aattctaatt tactatcaaa
     2881 ataatagtaa atagtggact tcttaattcc cgaaagaagg gagatatctt ctaaagtagc
     2941 ttcaaaaaaa ctcttttcgg caaaaacttt tcttgcccct tctaatattt tctctctttt
     3001 attcacctca attgcatggc cccccaagat taaatcccct acttaccatc taacccctcc
     3061 tcgcttgaaa agctgtcttt gtgatgacag agtagttttt atctcctgca aaacccaact
     3121 tttaacaaac ccacacctta tctcatagag ggattcttta cctcccaaca ttagttaata
     3181 aatatatgga tcggcttaaa tttcggtatt aaaataccaa gtctcttcag ctttacccta
     3241 aaaggtgtat tttacaccta aaaacactcc atcccccacc cccgctaact tactccccgg
     3301 agctccacta gaaaaagaat aagaaagaga aatgttccag tcgtcttctg gcttataagt
     3361 gagccctact acgtttacca tgcttccgtc ctgaagactc acgcctgagg taagcaaagc
     3421 agtccattta ttatcatcac tgggtctttc tgcagccaga aaaaggtagg gagaaatgtc
     3481 ttctccctct tcgtaagggt taccccacat ccacactaaa tttagcagag tattattatc
     3541 tgttgtataa tcaatgccca gacttccttt ccagtaagga ttttgaaaaa cttcttgagg
     3601 ataagaaatc acctcaccag agggctgagt tatatttatg cgatttatcc agcgctccgg
     3661 tacaaaaaga gcaatatctc ctcgaaaagt agaaccctcc agaagaggaa attctccctg
     3721 aaactccaga cccagcatac tccttcgggg atagcccatg gtaacatcta ctacgctgct
     3781 tccatcgagc ccgagaaaaa cattggtttc tcgaggataa ggagaaagat aataaccttg
     3841 caagtaactc accgccacat ctacgctgct tactatccct cgggctctca ctccccagat
     3901 aggatgagaa aaatcaacca atggctcctc tttagttaag ctcaaactca aattctgagg
     3961 ggggagcgag agtaaagaag ccagctcata ggctaaaatt tgtttttcca acccttcagg
     4021 aacaaaatta ggcacaaaat caggctgata gaaaaattcc agcgaccaat cccaggaatg
     4081 ataccattct aaatccaggg caggaacagg aattttattt ttcagagcat cttcactaaa
     4141 actcaaagag aaaggagtgg gatttataac atcaaccggg ctcataatat cgctggctcc
     4201 ccagctcaac tggagaagcc ccaagcgaaa gtccacctct tctgaggggg tctctattcc
     4261 ctgtaagaaa agctcgttaa tcatcaccgg ctgaagatag ccctcactct gataactgag
     4321 gagaagatcg gcagtaaagt aataattaga agatatagac gtatcgtatt ttaaatttag
     4381 acttcctccc caggcacttt cacctttctc taaatcgtaa gaagctccta ttttagcttc
     4441 tccggttatc tcactcgctt cccccgctac caggggaagc gcaaaaagga gtaaaccaaa
     4501 actcgccaaa atcaaaattc ttttaccatc caccttccct cacctcacag ctctggtcgc
     4561 tgaatggttc tcaccgagaa aaagttgtca ggaagaggaa tatctctttc tatatgctcc
     4621 atctccacga tggttttgtg gttttccttc aaatcagtaa ggtcaatttt attaaccgtg
     4681 atagttccgt cttcctcttc ttctatctcg aggatctcca taactttcca cagattaccg
     4741 tctttatcat aaaattcaat ccttcggaaa taaaacttct ctttatctac ttccatcagt
     4801 agtttagagt aactagattc ttcatcccga ggaataagtt ccagcttata aatttcctcg
     4861 gtttcttcta tcagagtaga atcataatct tctcgactgt agcttcctcc cagctcatca
     4921 taggtaaagt cggtgcccat aaatttctca cttttttctg aactggcaat ccgtcgcact
     4981 ttcttataag ccggcatgta aagatacatg ctctctcccc cttcgaggct gaggagagta
     5041 acatttttca cgtctgccgg gcttaaaaaa cgaacaatta tagaaataac gtcttctcct
     5101 tcgtctttag catacataat cacttctctc acttcttccc tacctttaga atctaccaga
     5161 atcattttag ctattgcctt ttgagtttca gactctgcag tacgggcttc tacctcatcc
     5221 agaatttcat ctgctgttat agcccaggcc gggacaacgc caattaaaat cactaacccc
     5281 cacaccatca aagatagtat ttttttcatt aaattcatcc cctttccatg tctttattct
     5341 ttttattgtt atttttatta ttaccatatt cagctgcatg aagaagcagc ggtaacacca
     5401 ttaaagtgta aaacatggta accagcaaca ccgtagccac tagatcacca aacatgcgaa
     5461 gcattcgaat acgagagaga gtaagaatat aaatccctcc tgagacggct aacgagttaa
     5521 gcactattcc tcttcccgta tgcaccagag tggtgcgaag aggattggaa gaacctttct
     5581 ccacttccat ataccagcga gaggtaagat gaatagtgta atcaattcct gctccaatgg
     5641 caatactggc taccatcaag gtggcggtat tcaaaggaat acccaacaat cccatgagac
     5701 caaaagaagt gtaaacagcc atcaaaatgg gaatgatagc aacaagagac tttttagcag
     5761 agcggagttg caaaagtagc aaaacgtaga taatgacaaa agccaggatt aaactttcaa
     5821 tctgccgggt aaagagctgc tgatttatgt gattggcaat gatgggtgtt ccagttattt
     5881 ggtaataaac caagatgtct ccattctctt gaggaagaaa aacatactca tcaagaacct
     5941 ggtaaaacac aaaatcaaaa tcgctatcta aggtttgacc caccagattt tctatctcct
     6001 ggcgtaaagc gctggcttta ttattccggg ctactcgttc catggataaa tctaaaactt
     6061 cagccagata taaagcatca tcttcggtaa tccccagatc ggtctccaaa accgaagaga
     6121 gcgagaaatg cgggtgagaa aaataggaaa gaagtgcctc ttcaacctct tcctcttcta
     6181 accccaaacc ttcaatttct aaaaaagcat catgggctac ttttttagca aattcctcat
     6241 caaaagccat aaaatcacgg agaggctgcc ctataaattg gaaaaccatt tgctctaccg
     6301 cttccggatc agagaaagat aaaccaaaaa tctccaaatc ctccactata gaggtagcca
     6361 gatattctgc tgctccctct tcggtaacgg acaccacctt aacttcttga ggtctttgag
     6421 ccaggaagga ttttaaggct tccacttcct cctccagctg gcgggaagta gtctctcgag
     6481 ctttaaactc tactaaagta gcatcctcat cttcacttat cctgtcttga atgaagctat
     6541 tatcttgagc aaaaaaccac aggttttcca ctttttcccg ggtatcagga atagctttcc
     6601 atccatccat agcttcattc aaattacaaa tgaaatcagc tatcgaggaa ggatcggcaa
     6661 aagtttcata gcgttcagca aagcgggaga tacgtatcat ctctctcaaa acaaagggct
     6721 cttttacttt ttctgtttct atatacaaat agttataact ggtgcctcca aagcgctctt
     6781 gaaaatattt caataattta gtaagagggt gattgactcc tatctgctct tctactgtag
     6841 tttccacctg aatgcgagga attcctattg ttaaaaccgc cagtgctact acaataatag
     6901 caattatcag gtttcgatgg tgggtaattc cccgagccat aaaaagcaag aaacggccga
     6961 tcaggtcttg agaatcctcc tcttcctcct gggaataata acgaggtaag cgacggggaa
     7021 caaaaacggt aaaaaaggct cccaagaaaa aagtggataa gagaaaacca aagaaaattc
     7081 ccagagtgga ataaagtcca aattcagtaa taggtttaat atcagcggaa agcaaagata
     7141 taaatccacc catagtagtt aaggcactga gaaggatggg aataaaaatt tcctgcagag
     7201 taactttaac aacatcatcc ccccccattc ttgaccgttc ttcataataa cggttaacaa
     7261 aatgaatacc ataagcggta accatggcta agaggataac gggaagggtg gcagatacaa
     7321 cggtaagaga ttccccaact agtgctgcta atcccaaagt ccagagggag gctaaaagag
     7381 caacgaaaac aggaagaata actccctgca aagaacggaa accccagtaa agaatgagca
     7441 gcaataataa cccagaaaga ggcgccagaa ctcttaaagt tctttgagca tcataagaag
     7501 tctgttctat tattatgggg agaccgaaat aagttatctg aactttctct tcataaggac
     7561 ggcagaccgc tcgtacttca tccactgctt cttcttcgtc tactgcctca taaaaagaaa
     7621 ctgctattat ggttccctta ccatcttcgg taaccatttt cccccagatc aatttgtctt
     7681 cttctaaatc acgacgcaac tcttctgctt cttcgggagt ttgaggtaaa acctccacta
     7741 cttcccttac ctctatgcca aaatcagtcg ctataacttt gggcatgttg gttagagcag
     7801 aaatgcttct tactgaagat agctcctcta tcgattccac tatttcctgt aggagctcca
     7861 tattgtgagg agtgaaaagg tcatcaaatt ccagagctac aatcatcgat ttcttctgga
     7921 aagaagcaaa tttgtcactc aaagaattat aaaaggaaat ctccgggtca tcttcgggaa
     7981 gatattcagt aacatcatcc ttaaacttaa cgtttactac ttgaaaaccg aagaaaatag
     8041 taactactaa aacagcaatt aaaattagcc aagcattttt aactatccat tgagaaattt
     8101 tctgcataaa tgtcttcctc cctaactgac cattcagtcg aaaatttacc ttcctataat
     8161 aaatgatagc gaataatttt tcaagagtcg aacaaggctt tctatcaata gtctgtgaaa
     8221 atgtcacgag ggggatatag cgtgatttgt atataaatag attttactaa ataatggtta
     8281 tagctatgga aaattaagtt gtataacgca cccctctaaa ctgaacagaa aaaataagct
     8341 gtattttaca gcctgtgatt acctatgagg tcaagaaaaa aatatgggtt ataaagatac
     8401 cttagatctc tttagtttat aatcacgata caataattaa actactttat tcctaaaatc
     8461 aaggtagcaa tgttaaagta aagcgttaac gctacaatat ctaccactgt agtaatgagc
     8521 ggagcagaaa atactgctgg atctataccc agcttgctac ctattatcgg cagcattgct
     8581 cctatagttg aacttacaac taaaaccaca aaaagagtga tagccaccgc gatagcaatt
     8641 tctactgatc cagcaacagc ccaggcaaaa aagtacccga ctatacccat cgtagcgccc
     8701 agcatagcac ttacctgaag ctctttaatc aaaattctcg ttgcatcttt agttgtgact
     8761 tcacccagag ccagagacct gataatcaaa gaagaagcct gagttcctga attaccacct
     8821 gtatcagtaa ataaaggaat gaaaaaagaa agagctacaa cactacttaa aacactttga
     8881 aaatgcttga gaatgttgct ggttaccgcc tgaacagcaa agagaaaaat caaccagtta
     8941 attcgcttac taaaaagggt gattaaacta gagtgcatgt aaggcaaatc cagaggttta
     9001 cttcccccta acttatgaat atcctcagta ctttcttctt caataacgtc cattatatca
     9061 tctgcggtaa taactcccac caggcgtccc ctgctgtcta caaccggaag aaccagcaaa
     9121 tcatactgac tcatgagttt tgccgcttct tcctggtcag catcaaccgg tatagccacc
     9181 acattggtgt gcattatttc cgaaataggc gttttaggat gactcaatag caattccttt
     9241 agagacacaa ctcccaccag ctgtccaatg ttgtcaacta cataaatgta gctggcaata
     9301 ctttctttac ggcctacttt gcggaaatgc tcgatcaccc gttcaactgt ccagcgttct
     9361 ctcactgcaa tgtattccag agacattatg ccccctgcag aatcttcagg gtaagtcata
     9421 agatattcaa ttttttgcag atactcttca ggaaccattc ccagaatttt ctgggcctgg
     9481 tggggatgaa tagccataac caggtctgct ataacatcac tgggcatcgc agcgattatt
     9541 tttctggcta cggtatcttc caaatggtca agaagccggt attgatcttc atactccaga
     9601 tgttccagag tttccgccgc catatctgta ggcatgagct ccaaaattat tagttgctct
     9661 ggttcttcta actcttccag cagtgtcgct acctcataag ggtgcagttc ttctaataat
     9721 tctcgtaaag cttcatagtc ttgagaagca agcatttctt cagcagactc tttaaaagat
     9781 acaatgtttc gctctcccac gagtgtcacc tccaatggca aaatttttaa aagctcaaaa
     9841 taactttatc gaaaatcgca atattcgatt cttatcctcg aacggacttt agaattcttt
     9901 aaggctgctg cggggccagc agatagaaaa gagaggaaag tatcagttgc ccgttaacag
     9961 agtttagccc aggctttcag aatacactcg atgcattcaa catatcagaa aaggcagtgg
    10021 ccctggcaca aaacctcaaa gaaaccagca cacaaagaaa tatatagctc acaatggaac
    10081 tttcgatatt taatcgctcc tggcatctat gactaccagg ttcggtgtcc attccatacc
    10141 accaccttca gcaacacaaa aataaccgtt cataagttta tcacacctat aaaaaaattg
    10201 cagtcatcac caaaaataaa tccaattcag cccgaaatat agaatatgag gataactttt
    10261 aagaggaaaa aagcgcgaag cagagaaaat catagtgtta gagagtctta agatgcaaaa
    10321 agaagggtga cataaatttg tagagaacgg gaaacaccag aatttgccct acaaatagca
    10381 cgataggttc acagatgcga tactaaaaga cttaaagagg tttccaatcc agagcttcct
    10441 ccataacctt aagcggtgct ttttccccgg taaaaagaaa aaagctctct gctccctgtt
    10501 gaagcaacat ttcttttccg tcaaagcttt ggattcctct cttttttgct tctctgacta
    10561 aaggtgtcag accccgccga taaaccacgt ctactatcca aaccgaagga gagagtttct
    10621 cccagggtag ggatatttct tctcccttta aacctaaaga agtggcgttc actatcactc
    10681 ctgcctcctg ccagagaggt agggagtctt gttctttcat ttttccccat tccaccactt
    10741 ctacctccag aaaaaattcc gctcttgccc aattggccag gtcttctccc ctctcccgag
    10801 ttcgattaat gacaaaaata gattttaccc cccttttcca tagagcccaa actgctgctt
    10861 ttgccgcacc acccgcccct aaaagcaaag cttttttacc ttctacgttt accccttttc
    10921 tctccaggct acgatacaga cctaccccat cggtgttata accaatcaat tttccctttt
    10981 cattaactat ggtattaact gcctctataa cccgggcttc tccttctaag gcatccagga
    11041 agggaattac tttttcttta taaggaatgg taacattaaa acctctgata gagaaaattc
    11101 gcatagcctc cagcgccctc tcaagattag agggtaaaat atcaaaagct aaatagacaa
    11161 aagggagacc aagataagag agagcagcat tttgaaaaag aggagaaaga gaatgttcaa
    11221 tggggtagcc aataactccc agaagccggg tttgagcagt aatcatgctt ttagcctttt
    11281 cagcactcgc ctttctaacc gccttaccac acgggtgaaa aatagctcct tctcatttac
    11341 cggtgaaaca agaatggtaa aaagacccaa acgattcccc ccccacacgt cagtaaaaag
    11401 ctggtcacct atcaccgcta cttgattttt ttctaaaccc atttgcgcca gggccagacg
    11461 aaaagcaccc cggcggggtt tttgagcctg ggaaatcccc ggaatgtcga ggctctctct
    11521 aaaatgattc actctctcct ctaaagcatt agaaaccagg aaaattttaa aaccccgctc
    11581 ttgagcctcc tgtatccagg ctttcactcc cggaggaaat tcaaaacctc cccagggcac
    11641 cagggtatta tccagatcga taattaagcc ccgaatacct tttttcccaa gaaaatctaa
    11701 agaaatatca gaaagacttt ctgtcataag gtcaggggta agttttttcc accagctcac
    11761 ggattattac cctctttata atgtagatgc tcttcataag tttgggaaaa agcatgtttt
    11821 ccatcacctt gtaaaacaaa atagagataa tcagtctttt gaggataaag aacagaaagc
    11881 aaagaatcta aacccgggtt acatatagcc tgaggaggaa ggccagaaat gagatatgta
    11941 ttaaagggag aggcaacttt caaatcctct tccttcaggt tttctacttt taatcctttt
    12001 tctcgataaa gagcataaac cacagtggcg caagactgca gcttcatgcc ctgtttcaag
    12061 cgattgataa aaacggaagc taccacaggt ttctcctcct tccgctttgc ttccttttct
    12121 accatggaag ctaatattac cacttctttt aaggataaag aagaacgaga taaccaataa
    12181 tgaggaagaa agacctcttc aaaatggtgc agctgggttt ctatcacttt ttgaggagaa
    12241 gtaaaaggag aaaaataata agtatcaggg aaaagaaaac cctccaaatt ggtgattcct
    12301 cccaaccaac cagccgaaaa aagctctggt ttatttatga gatccagata atctctggca
    12361 gagcaaatac ccttttcttc taaaactctt gccatttgct cggaggtaaa accttctgga
    12421 aaagtgattt tttcataact gggaccctga gtcaactttt taactacctc ccctaccgct
    12481 tcaccggggt ataaaaaata gttaccagct atcaacttat cctgattgga taaaaaaaga
    12541 aagagtcgaa aaagaaaaga aggaacgata ccttcctctt ccagaatata ggatatttct
    12601 cgtcccgaaa gaccattctc aattactact attttccttt ctccttcttc cccaccataa
    12661 taaaaagcca acgctaaaaa aagacatagt agccagctga gagaaagata aagaatgaac
    12721 ttagacctca tttcccatgt gagaaagata agtttccaga atcaaagcag cagaaacttt
    12781 atctataacc tttttccttt ttcttctact gagatcagct tctatcaatc tcttttccgc
    12841 ttccttagtg gataacctct catcccacaa cactacctca ccctcaaaag actgttccag
    12901 ttttttctta aatttctcca ccctccgggc ttcttcctga aaggaggcat caagatgaag
    12961 aggtaagccc accactatta cctcagcttt tagctctttt gctaaagcta aaattttatt
    13021 tatcgcctct tcgccctctg cttctattac ctcacagggg aaagaaaaac cggttccctg
    13081 attaaaagcc actcctatcc ttttttctcc ccaatctata gccattactc tcttcataaa
    13141 ctctctaccc agtcttctaa aagagaaatt agctcttccc atttttcctc cgataatcct
    13201 ccaaactggg ctaaatgagg cctcccccct cctccccctc ctattttctg gctccactcc
    13261 tttatcattc ttcccaaatt tgcctccggt ggtaatccta taccagccag aaccccctgg
    13321 atttttcctt ctttctttaa gcctaaaact actactccct tctggataaa aggccgaagg
    13381 tggtctacta cctccttcag aaaatcaagg tctaacccat cctcttctcc caaataggaa
    13441 acaagagtgg tttcctcgtt actgactgat gatatctggc tcttaatctt ttcttctatt
    13501 ctccttcttc tttctttttc cagagctttt ttgagttctt tattctcacc taccactttt
    13561 tctatatact ctttcacttt tccctcttcc acattgacag ttttaacaac atcctgtaag
    13621 atttgctcct tctgattaag ataagaaaga gcagaagggc cagctactcc ttcaattctt
    13681 cttaatcccc ttccaatgct ggattcagac gttatcttaa aaagcaaggc ctgcgcagtg
    13741 tgagcaaggt gagtcccccc acagagttct gctgaatagt ttcctactct aactattctt
    13801 accatctctt catatttttc ctcaaaaaga gctatcactc ctcttttctg agcttcctcc
    13861 agagtggaaa aggtaatttg caccggtaaa tcctcgaaaa tcttttcgtt aaccagagac
    13921 tctactgctt ttatctcctc cgaagataaa ggggcaaagt gagtaaaatc aaaccgtaat
    13981 ccctcttctc ctacccagga gccggtttgt ttaacctgct ctcctaaaac ttctcgcaga
    14041 gcacgatgga gaagatgagt aactgtgtgg tgaatctcca tcgcttttct tcgccgagcc
    14101 tcgatttgag cccgcgcttt ttctccttcc ctcacaattc cctcttccac cactcctcgg
    14161 tggataatca aagaggaaga gggagcgctt acttgctcta cccttatttc cccttgcgaa
    14221 gaagtaataa agcctttatc tccttcttgt cctcctcttt caggataaaa aggagttttt
    14281 tctaacacta ctattacctc ttctcctttt ttcacttcct tcactctttc tccctgagaa
    14341 aaaagagcta aaacttgagt ctctatttct ttttcttcat agcctacaaa ttcgctttcc
    14401 aggccagaaa agatttcttc ttcatctttt ctctctttaa attcttgagt cttttcttca
    14461 taagcccgac gggctctggt tttttgtctt tccatttccg tttcaaactc ttcttgagaa
    14521 aaagacaaac cttcctcttc taatatttct gaagctatat cagggggaaa accataagta
    14581 tcataaagat aaaatatttc tcttcctgga attactcttt cctctttttc ttgaaagcga
    14641 gaaatcaaat ctttcagata tcccattccc agctgtagag tttcttcaaa ctttctttct
    14701 tcctggagca ccacttgagc aatcaatttc ttcctatctt tcagctctgg ataatgatgg
    14761 cccataatct ctatcaccac aaaagccaat tccggcaaaa agggatgata cagagaaagt
    14821 ttatgcccat aacgatgagc ccggcgaatt aaacgacgca aaacataacc tctgccttcg
    14881 ttcgcaggat aaactccatc ccctattaga aaagtaatgg ctcgaatatg gtcagcaatg
    14941 attcgaaaat agggtcgtaa atcttctctc tgatagctaa ttccactcat cttttctatt
    15001 ttttcaatta tgggcaaaaa aagatcggtt tcaaaatcag tttgagcttt ttgcattacc
    15061 gaggtaatcc tctccaagcc catgccagta tcaatattct tatggggtaa ttctcgtagt
    15121 aaaccatcat tccctcggtc aaactccata aataccaggt tccatatctc taaatatcgg
    15181 tcgcaatcac aacccgggcg acaatctggc tgtccacatc ccctctcctc tcctgtatca
    15241 taataaattt cggaacaata accacaagga cccaccggcc ctgaagccca aaaattatct
    15301 tcatccccca gaaatactat cttttcctct gaaatgccca cttcctctct ccaaactgta
    15361 aatgcctctt catcccgatg gtgaacggaa atccataatt tatcgggagg aagcaatagc
    15421 ctttgggtga gaaactccca ggcccaggaa caggcttctt ttttaaaata atcaccaaaa
    15481 gaaaagttgc ccagcatttc aaaaaaagta tgatgacgag aagtatagcc tactttatcc
    15541 aaatcgctta tccgtacaca cttctgcact gtaactgctc gatgaaaggg aggtgatact
    15601 cgaccttcaa aaatagattt aaaaggcacc ataccggcaa tagtcagaag tatagtaggg
    15661 tcttcgggga taagagaagc actgggtaaa attttatgtt ctttttcttc aaaaaactga
    15721 agaaaggctt tcctgatttc ggcactggtc atagtttgta ttctcctttt taaaatccac
    15781 tacttgaaaa gtatgaagtc ttcgattaga agagggcatc cagcgaacag taataggaaa
    15841 ggcaaattca ggttgtagct ttcctttttc caaccaataa agggaggaaa gattacgata
    15901 ctgtcctaaa ttagctctca ccaccaaagt aataggacta tccacccagc taacttcatc
    15961 tatttcctct atagaagaaa gaacagcagt tatttcctcc ctcaaagcta aagaagggag
    16021 gggtttaaca ttaaccagag caatattgcg gttaaggggg ccaattattt tcacattagc
    16081 tatatccctt tctatgtttc tttcattcaa tattttgagg agatactcgt tgctctttac
    16141 caccgcctgg gcttgataag gagaagccaa gggttttaaa agatgtttta gtagacatcc
    16201 ttctcgaagc ttattctccc ccattcttcg taagggaaga gagagaaaag aagccagatt
    16261 tacaatttct tctttactaa gttcaaaaag gggagaataa gtgttattaa gtagctttac
    16321 ccctcttttc ccccagctat cactctggtt agcgcctcct acaattaaaa ccctatttcc
    16381 aaaataacgg cgaatagttc ctaactttgc ttctttagtg cagcgattgc aggcgggacc
    16441 ggttttcata atctcttcca tcacttttcg cccggggaga taaacatgat ctactcccaa
    16501 attatctacc cagtagtcaa cactctcccg agctaaagaa ggaaaataaa tatcccaatc
    16561 cacggtacaa gatattactt tttcttttcc caaggcctga agagaaagaa aaagggcaag
    16621 ggtgctatcc attcctccag aaaaagccac taccactcgc tcaaaagagg aagacacttc
    16681 ctttagttcc tggataacat cctgagcccg tgttagagag gggttggtcc gatctgtcat
    16741 tttttgagac tgtttaaaaa atcagttaat ttacctccca ccatatctcc taaagttatt
    16801 ttaccgttgg aagatgcata atccttctct tcctcttgtg aagcttgttt gatactcagg
    16861 ccaatctttc tttcctggtc gtcaagctta ataactttta ccttaacttc ttcaccttct
    16921 tttaatactt cagaaggaga agaaatccgc tggttagaaa tttccgagat atggactaaa
    16981 ccatcccacc ctttttctaa ctctataaaa gcaccaaaat ctaccaatct aactatcttc
    17041 ttgagatgaa cactgcccac gggataacta ttcttgattt cttcccacgg gtcaggaagg
    17101 gtcgcttttc ggctaactac gattctctgt tcttcaggat taaactctat aattttgaga
    17161 gataaggttt gaccccgctt caggatctga ttaggatgtt taactggctc ccaatccagc
    17221 tccgaaagag gaaggagagc ttccacatct ggaaccaact ctacaaaagc accaaaattc
    17281 atcagtctcc gcactttcgc ttctaccatg tctcccactt tccactttcc agcaatctct
    17341 tcccaggggt taggacggag ctgtcgaata ctcaaaagga tttgtcgatt ctgggtatca
    17401 acttctaaaa ttttcgcttc cactacttga tttcttctaa ctatctcgct aacccggtta
    17461 actcttttcc atgataactc ctcaagcggt attcgtcctt ccactccttc ttcaatctct
    17521 ccccataccc cacgaggagt aattgaagat actctcaacc ggacagtgct acccaaggga
    17581 tatttctcat ccacttcttc ccaggggtca ggcaaaacct gtttaatgcc cagagagact
    17641 cttctcttat ttttatctat ttccaacact cgagcttcta ctatactacc taccttgacc
    17701 acttcttgag gatgtttcac ataaccccag gaaaggtcgg aaatgtgaat taatccttct
    17761 aatccttctt ctatttctac aaacactcca aaatcccgaa cagaaactac tttgccttta
    17821 actatatcac cgggccttat tttctcatct atatcttccc atgggtcagg ttgaagtctc
    17881 ttaagactta aagatatttc ttccttctcc ggatcaaaag caataacctt tacttctatt
    17941 tcttctcctt tttggaagag atcttctaaa tttccaaagg tttttttcca ggaaacttct
    18001 gaaacatgta aaaggccatc aactcctccc aaatctataa aaactccaaa tttggtaatg
    18061 ttcttcacta ttccttttct cacctgtcct acttccagag aatcaaagag ttccttcttc
    18121 tgacgctctc tttcttcctc cagaaccgcc tttctagaca ccaccacatt ctttgtcttc
    18181 ctatcagctt caatcacttt aactggaata gtttgatcta taaactcatc caaatttttg
    18241 ggagtaatat caacacaaga ggcaggtaaa aaggcattga ttcccacatc tacaatgagg
    18301 ccacctttta ccctcttttt aactttggcg gttaatgttt tattctcgtt ttgactctcc
    18361 tctatatcta tccaggctcc ctggtaacga gcttgctctc tggagagcca cacataacct
    18421 tgctcatcaa tgtgagtaac tactacccac gtttcatcgt cttcctctaa ctgattttcc
    18481 acttctcctt ctttgcccac cattttttct cgcaaaggca acagaccttc cgacttggag
    18541 tttatgttga caaagtaatt ttcctctcct tttttaatca ctttgccttt tacgaggtca
    18601 ccaggtcgga caatgcggat ttcacctaaa tcttcatgtg ttaactccat tctctcacca
    18661 ttttccacaa ccaaacaact ccttcctcca tttcctttac actaaacggc cccgctttgc
    18721 cgtgtgccaa ccgctttgat cctcgccctc aaaaaggtgg gtgccctccc aactgaacca
    18781 cggctcaatt agcgaacagg ctcccaagac aaggatgttg gttcaaagcc ttctctgaca
    18841 tcaccaacaa agcagggcca catccattcc aaaccccatc aaaatgatag cacaagatgg
    18901 tgctcttttc aaccaaaggt agatgaatgg ctaagcatct tcccgaggag gacagatact
    18961 aaaaatatag agtctatagt taa
//
LOCUS       DAOPSS010000017        18184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2589_length_18184_cov_8.675547, whole genome shotgun sequence.
ACCESSION   DAOPSS010000017 DAOPSS010000000
VERSION     DAOPSS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 18184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18184
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2589_length_18184_cov_8.675547"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="PKN31_02090"
     CDS             complement(<1..1867)
                     /locus_tag="PKN31_02090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOA98754.1"
                     /translation="MRFENREQLEKYIIELKNKNGKKKVLICGGLGCLAKGAEEVLDT
                     FRKVIEREKVEAEVEKLEESPHLVKTGCMGLCEAGPLVRIEPEGILYLRVKPEDVEEI
                     VDKSLKEGEVVERLVYQEKVNGEVHYWPHLKDVPFYRKQVKIALRNCGSIDPQNLDEY
                     LARDGYLALAQVLNEKTPDEVIEEITKSGLRGRGGGGFPTGRKWQFARVVKADKKYII
                     CNGDEGDPGAFMDRSVMEGDPHSILEGMAIAGYAIGAQEGYIYVRTEYPLAVKHLRKA
                     IEDAERAGFLGDNILGSDFSFRIIVREGAGAFVCGEETALIASIEGKRGMPNPRPPYP
                     AYRGLWGKPTVINNVETLTNVPIIMLRGADWFRSFGTASSAGTKTFALAGKIKNTGLV
                     EVPMGVTLRDLIFGIGGGIQDDKKFKAVQIGGPSGGCLTERHLDLPIDYDSLSGAGAI
                     MGSGGLVVLDEDTCIPEVARFFMNFTQNESCGKCVPCREGTKHMLGLLQKVVKGEAST
                     SDLELLEETAQVVRDSSLCGLGKTAPNPVLTTLFYFRDEYLDHVVHKVCPAHICQAMK
                     EYRIDPEKCRACAKCARVCPVSCISGRPRVPYVIDQEKCTKCGACFEACPFDSIIV"
     gene            complement(1857..2372)
                     /gene="nuoE"
                     /locus_tag="PKN31_02095"
     CDS             complement(1857..2372)
                     /gene="nuoE"
                     /locus_tag="PKN31_02095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOA98755.1"
                     /translation="MEIEKESLVLSRQFEKVNAILRKYSHNVDNLIAILQEIQSEYRY
                     LPEEILTYVATALQVSPAHVYGVATFYAQFSLEPKGEHIIRVCDGTACHVKGSAQILK
                     AVRENLGLREGENTTSDLMFTVETVACIGACALAPALMVDEEVYGQMDEEKVQQLVDH
                     LLGEGEKVDAV"
     gene            complement(2406..3560)
                     /locus_tag="PKN31_02100"
     CDS             complement(2406..3560)
                     /locus_tag="PKN31_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOA98756.1"
                     /translation="MKDLFVEFAYAQLPKWGEELCGDSVSYDKGNNSTILAWSDGLGS
                     GVKANILSTLTSKIIVSMLKRESTLEEVIATLAETLPVCKVRKIAYSTFSVVQVLSSG
                     ETYVAEFDNPPLFWLRNGRLMHLDRKEREIGERVVLESIMRVQKNDWIVGVTDGVVHA
                     GIGGLWNLGWRWEKIASFLENTVNDQLDALTLANKVIATTKRLYQDRLGDDATCVVAK
                     IRLPRYLIVWAGPPEDPALDEYIVKRVMEFPGKKAVCGGTTGNILARVLGKEIEVDLT
                     SLQKDIPPVGMMEGVDLLTEGILTLATVLRRLRKGVELEEVIYQRDGASRLLSLLLEA
                     DEITFIGGRAINPAHQNPKLSGELSLKARLLEDIVSELQKRGKIVNLEYY"
     gene            complement(3557..5275)
                     /locus_tag="PKN31_02105"
     CDS             complement(3557..5275)
                     /locus_tag="PKN31_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOA98757.1"
                     /translation="MGVISTIEARCRDCYKCIRNCPVKAIRVSLGHAEVVDERCIKDG
                     RCVLVCPQDAKKIQNDLPRVQELLQGEDPVVVSLAPSFVAAFSEVTEGQIVAALKKLG
                     FQEVRETAEGAEWVAREHARLLDEGKRNIISSSCPAINALIYKYYPQYVSYLAPVVSP
                     MVAHARLIRQEYEKVKVVFIGPCIAKKAEIEEEEVKGEVDAVLTFEELKTWFQEENIS
                     LSALEEEEMGLSSVEWGRAFPVEGGLLKTASLDTGILSKEVMVITGIDRCRQFLDDLK
                     DTKGDFEMVEMMSCEGGCIDGPVLRSSLSPYERRKKVIDYTEKAKMASNPPPEKKIIS
                     VPTFSRNFAPHPIPQPEVPEEEIKRILSLTSKFSPQDELNCGACGYNTCREKAQAVYQ
                     GMAEAEMCIPFMRARAESFSSFLIAVTPNGIVLVDENLRIVDINPALRRMFGLTGKLV
                     VGGKLDQLTDPSPFVEALRTKKMVTREVFYPQYQNLYVQLSVFYLEKSQLLMGVFVDL
                     TKEKEREVLMEQIKEETLEKAQQVINNQMRVAQEIASLLGETTAETKVLLDKLMKILR
                     GESVEG"
     gene            complement(5262..5564)
                     /locus_tag="PKN31_02110"
     CDS             complement(5262..5564)
                     /locus_tag="PKN31_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOA98758.1"
                     /translation="MFFFLGEVRMVTISVCVGSSCHLKGAYEIIEICEELIEKLKLKD
                     QIELKGNFCLGRCTEEGVTVMVDDEILSGVSPQNFREIFQEKILKKLGEDGTDGSN"
     gene            complement(5697..6329)
                     /locus_tag="PKN31_02115"
     CDS             complement(5697..6329)
                     /locus_tag="PKN31_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA98759.1"
                     /translation="MKIRWFGHSFFLVTSGDTKIAFDPFDDSVGYPLPQVEADLVVVS
                     HDHFDHNNVSLVRGHPEIIKEPGVYEKNGIKVEIFSTFHDEVGGKKRGKNNVARLELE
                     GMSLIHAGDLGIIPPDEELSSWKPVDILLLPVGGIFTIDAQEAQELVSKLSPHLVIPM
                     HYRTEYTKFELNPLEPFLQGFREVKRLGSSEVEVTREILPTNLEVWVLEI"
     gene            complement(6622..7407)
                     /gene="trpA"
                     /locus_tag="PKN31_02120"
     CDS             complement(6622..7407)
                     /gene="trpA"
                     /locus_tag="PKN31_02120"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOA98760.1"
                     /translation="MADILKEKLEERRKKGKLLIPYLTFGYPDIPTFLDLIQICEEEG
                     VDALEVGIPHSDPVADGPVIQTTSQRALEKGVTPPLVVETLRKERTALPLIAMTYGNI
                     VYQYGFSRFAQDFKDAGFSGLIIADFPWEARHFEKEMEGYLSRVLLASVTSSPARIKQ
                     IARESEGFVYLVSGKGTTGQVEMEEERLREVVELIKEVSSSLVLIGFGVNSPEKAQKL
                     AQIADGVIIGSALLQVIIQEERSPQRGFRRLLSSYCQALDKTS"
     gene            complement(7400..8584)
                     /gene="trpB"
                     /locus_tag="PKN31_02125"
     CDS             complement(7400..8584)
                     /gene="trpB"
                     /locus_tag="PKN31_02125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOA98761.1"
                     /translation="MEKAGFFGEFGGKFVPEILIHPLEELEEAYEYYRKDEKFQEELN
                     YYLKNYAGRPTPLYLAERLTESWKGARVYLKREDLNHTGSHKINNTLGQALLAKKMGK
                     KRIIAETGAGQHGVATATVAALLGLECQVYMGAKDVERQKLNAFRMKILGAEVIPVYS
                     GSQTLKDAINEAMRDWVRSLDTTHYVIGSVVGPHPYPQMVRDFQSVIGRETKKQIVEK
                     EGRLPDYALACVGGGSNAMGMFYEFFPDNVALIGVEAGGRGIETGKHAASIGGGRKGI
                     LHGSLSYVLQDEFGQIQETHSVSAGLDYPGVGPEHSYLFSSGRAQYVSVTDEEALQAF
                     LELSKKEGIIPALESAHAVAYASKIAPHLPGKVMVICLSGRGDKDVEEVLRYLEEEKR
                     NG"
     gene            complement(8586..9191)
                     /locus_tag="PKN31_02130"
     CDS             complement(8586..9191)
                     /locus_tag="PKN31_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOA98762.1"
                     /translation="MPRVKVCGVKEKRDVLLLGEFPIWALGFILVEDSPRFILPEKAK
                     EVVNSLPSRIMKVGVFQNQSVEEVNKLRELCSFDLIQLHGEESPVFCEKLGKGVIKAF
                     SVGEDFDVAMVEEYLPVVDFLLFDSKKAGSGITFPWEKIKGISFPKPIIIGGGLKEEN
                     IWQCLSLLHPFALDLNSGVEIYPGKKDPEKVKRILEKIGGK"
     gene            complement(9178..9978)
                     /gene="trpC"
                     /locus_tag="PKN31_02135"
     CDS             complement(9178..9978)
                     /gene="trpC"
                     /locus_tag="PKN31_02135"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOA98763.1"
                     /translation="MMEGFLEKIVVGKKKRLLEGKQYSSREVVDVFSEREKKKNTLLS
                     SLRNSPEPNIIAEIKKASPSRGVLIEGSFLPFLEEYEEGEAVAISVVTEEDYFQGSLE
                     MFREVRKITPLPLLRKDFVIEETQIYESAQNGADALLLIAGLLSRERLQRFVYLCTLL
                     HLLPWVEVHDEEDVEKALQAHAPLIGINNRNLATFQVSLQVSERLCRKIPPSVIVVAE
                     SGIKSRNDIKRLMDKGINNFLIGETLITSCHPAEKLRELRGGVEVAPG"
     gene            complement(9975..11009)
                     /gene="trpD"
                     /locus_tag="PKN31_02140"
     CDS             complement(9975..11009)
                     /gene="trpD"
                     /locus_tag="PKN31_02140"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOA98764.1"
                     /translation="MLKTARDFLEYIIEGKRLTRKDSYSLMRGLMEGEFTSSQMGALL
                     SSLRMRKEDGEEIAGFAEAMREKAMNFSVSSLSVADNCGTGGDGQGTFNISTASAFVA
                     LSSGLKIVKHGNRSVSSRCGSADLLEILGIKVDMEREKMQTLFEETGFAFLYAPLYHP
                     ATRTVQGVRKELGIRTVFNLLGPLTNPCPMDYKLVGVYSPELIEPVASTLATLGVKRG
                     LVVWGEPGLDEISTTGESKLAFVEGGKITFSSFHPEEAGFPVRNIEELRGGTPAENAR
                     IFVNILGGEEKGARREAVVLNSAFLLWIGEEAQDLEEAIEKVEEAIHSGRALQTLKRV
                     VKVSRALEGE"
     gene            complement(11003..11579)
                     /locus_tag="PKN31_02145"
                     /pseudo
     CDS             complement(11003..11579)
                     /locus_tag="PKN31_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
     gene            complement(11579..12982)
                     /locus_tag="PKN31_02150"
     CDS             complement(11579..12982)
                     /locus_tag="PKN31_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOA98765.1"
                     /translation="MEIKPDREKFKKLSAEGYDFIPLYAEQLADRLTPVSLVDKFRGE
                     TPLILLESAERGEKWGRYSFLIRGGEEVKIEDFTDIISLLEREFASYSVYPEPSLPFL
                     GGAVGFLSYDAVKTWEKTISRRKISYPLAYWIKSNRFLYFDHLKHLLGAVYVARGGEE
                     EDYNQGKKWIEETYERIGQSSSPSSFFATKGEVECALSEEEFREKVEKVRDFIREGHA
                     SQVVISQRFSQAFSGDPFIPYRFLRSLNPSPYLFYLETDQFQMAGSSPEMLIKIENGK
                     IFTKPIAGTRKRLSSIPEEEIIGDLLNDEKEIAEHVMLLDLGRSDLGRVCDRGTVQVE
                     KFMDIERYSHVFHIVSTVSGRLAPCISPWRALQASFPAGTVSGAPKVRAMEIIEELEP
                     LPRGPYAGALGYVSLNGNLDTCIIIRSIFFREGKAEVQAGAGIVYDSLPQKEYEETRS
                     KAEALLLALNLAGRGND"
     misc_binding    complement(13010..13257)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13469..14254)
                     /locus_tag="PKN31_02155"
     CDS             complement(13469..14254)
                     /locus_tag="PKN31_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOA98766.1"
                     /translation="MKKVVRVLVDPPLSGYENMSRDEALLEMGFPDAPLTLRFFEWEE
                     ETLSVGRFQKTAEMDFSYLEAKNIPLVRRPTGGRAILHDKEVTFSLFIPTSSFEKHTF
                     YYKELQKVLTRMMEKMGIKVDEKTESGIYTSSPYCFSINVAHEITIGGRKIIGVAQAR
                     GERGNLFQASFILEGNREEVASCFKKKEKIQKELENSLLALCEVLSPLPEKRKIYRLL
                     LESLREEWGVEIEEGEWSVEELEKAEELFSTKYYPGSWYHTER"
     gene            complement(14251..14631)
                     /gene="gcvH"
                     /locus_tag="PKN31_02160"
     CDS             complement(14251..14631)
                     /gene="gcvH"
                     /locus_tag="PKN31_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOA98767.1"
                     /translation="MNIPDNLLYTREHFWVKKGDLATCGITDYAQNELGDVIFVELPE
                     EGKKVKQGDKIGEVESIKTVSYLYAPLSGEVMEVNRELDKKPELINQSPYGEGWICKI
                     QPENEEEFKNLMNKEEYEVFLKTQ"
     gene            complement(14637..15728)
                     /gene="gcvT"
                     /locus_tag="PKN31_02165"
     CDS             complement(14637..15728)
                     /gene="gcvT"
                     /locus_tag="PKN31_02165"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOA98768.1"
                     /translation="MTLRELPLSDFHRSCGARMGVFCGWEMPLYYRSIREEYFACTNS
                     CGLFDISHLGKFLLRGKEATPFLDYATCRKVSEIKEGRGKYIFFLNWRGGIVDGATLF
                     REKEEEWLLISNAGAREKLFRWLDYLIKTNKWEVEIEDVTEKKALFAIQGPEAAVIAK
                     ELFQVSGDNWPKFSFRKFPPGFQVTHLAFSKEDGFEIMSDVSLGSGLWEKLFKEVERR
                     GGALCGLGARDLLRFEATFPLYGQEIDETITPLELGREDLVDWEKADFIGKKPLLLEK
                     EEGSKKRLVGLEILGRRIPRYGYPLYTKEGMPCGYVTSGNYSFSWHKVLALGFISSIS
                     GEKGETLLVAAPRTRLEAIIVNLPFGEGG"
     gene            complement(15740..17128)
                     /gene="lpdA"
                     /locus_tag="PKN31_02170"
     CDS             complement(15740..17128)
                     /gene="lpdA"
                     /locus_tag="PKN31_02170"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOA98769.1"
                     /translation="MKEAEVVVIGGGPAGYAAATRAGREGWETILVEGRELGGTCLNR
                     GCIPTKVFFKSQEVANLVKKAQDFGIKADFQGVDWEKVLNRKNRVVKQLTSGVQFLLR
                     KGKVEVIEGFASFLDSHTIRVEKEGGEKEEIRGKYIVLASGSRSATLPVEGANLEGVI
                     DSDQALELSSLPESLVVVGGGVIGMEMACIFNAFGVKVEVVEMMPKILPPVDEEITNL
                     LTQLVEKRGMRVHVNAQVKKIHRSGNLRVTFIQEGEEKEVEGDCVLVATGRVPQVENL
                     GVENLGLKMENRGVWTDEFLRTSISHIYAPGDVNGKYLLAHVAYREAEVAINHIKGEK
                     KGISYKAIPNCIFSFPEIASVGLTEKEAEAKGYEVKVGRFPFRANGKALIEGETEGLV
                     KIVSEAKNGEILGVHILGPHASDLIGEAVLALNLECTPQEIAETIHPHPTLSEALMEA
                     AQGVFASPLHFS"
     gene            complement(17130..>18184)
                     /locus_tag="PKN31_02175"
     CDS             complement(17130..>18184)
                     /locus_tag="PKN31_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA98770.1"
                     /translation="ADYEREKGVKAPSLPPPRDKKVAVIGSGPAGLTCAGDLARMGYK
                     VTIFEALHAPGGVLVYGIPEFRLPKAIVAQEVDYVQSLGVELKVSRVIGKTFTLDDLK
                     DEGYQAFFIGTGAGLPYFLEVPGENLNGIYSANEFLTRSNLMKAYLFPRYDTPLKVGQ
                     SVAVIGGGNVAMDSARTALRLGAKEVCLVYRRTKKEMPARIEEVERAEEEGVNCIILT
                     NPVRFLGNENGWVTGIECIQMELGEPDESGRRRPVPIPGSEFVIPIDTVVVAIGQGPN
                     PLLLETIPGLQLNRRGYIAADAETGATSLAGVFAGGDIVTGAATVISAMGAGKRSAQA
                     IDRFFTDSNSFPEWRQ"
BASE COUNT         5094 a         4674 c         3088 g         5328 t
ORIGIN      
        1 ccacaattat ggaatcaaag ggacaggctt caaaacaagc cccacattta gtacactttt
       61 cctgatctat aacataaggc acccgaggac ggccagaaat gcaggatacc ggacaaactc
      121 gcgcacactt ggcacaagca cggcactttt ccgggtcaat cctgtattct ttcatagcct
      181 ggcaaatatg agcagggcaa accttgtgga ccacatggtc taaatactca tcccggaaat
      241 aaaaaagagt ggtaagaaca ggattcggag cagttttgcc taaaccgcac aaagaggaat
      301 cccttaccac ctgggcggtc tcttctaaaa gttccagatc agaagtgctg gcttctccct
      361 ttactacttt ctgtaacaag cctaacatat gtttagttcc ctctcgacaa ggaacacatt
      421 tgccacaaga ttcattttga gtaaagttca tgaaaaagcg agccacttct ggaatacagg
      481 tatcctcatc taatactact aacccaccag accccataat agcccctgct ccagataggg
      541 aatcgtaatc aatgggtaaa tcaagatgtc tctcagttaa acatcctcct gaaggccctc
      601 caatctgcac cgctttgaac tttttgtcgt cctgtattcc tcctccaata ccaaaaatca
      661 aatctcgcaa ggtaactccc ataggaactt ctaccaaccc ggtattttta attttaccgg
      721 ccagggcaaa agttttagtt cccgcactac ttgcggtgcc aaaagagcga aaccaatctg
      781 ctccccggag catgattatc ggcacgttgg tcaaagtttc aacgttattg atcactgtag
      841 gtttacccca cagaccccga taggcaggat aaggaggacg agggttgggc atacctcttt
      901 taccttcaat agaagcaatc aaagctgttt cttctccgca gacaaaagct cctgcccctt
      961 cccgaactat aatccggaaa gagaaatcac tacctaaaat gttatctccc agaaatccag
     1021 ctctttctgc atcttctata gcttttctaa ggtgcttcac tgccagagga tactctgttc
     1081 gcacataaat atatccttcc tgtgctccta tggcataacc ggcaatggcc ataccttcta
     1141 aaatagagtg ggggtctccc tccatcacgc tcctatccat gaaagcgccc gggtcacctt
     1201 catcaccgtt gcaaataatg tattttttat ctgctttaac cacccgagca aattgccatt
     1261 tcctcccggt ggggaaacct cctcctcctc ttccccgcaa accagattta gtaatttctt
     1321 cgataacctc atcaggagtt ttttcattga gaacttgagc taaagccagg taaccatccc
     1381 gggctaaata ttcatccaga ttctgagggt caatagaacc acagttgcga agcgcaattt
     1441 taacttgctt tcggtaaaaa ggaacatctt taagatgggg ccaatagtga acttctccat
     1501 tcactttttc ctggtaaact aatctttcta ccacttcccc ctctttcagg ctcttatcta
     1561 ctatttcctc tacgtcttca ggcttgaccc ttaaataaag aatcccttcc ggttctatcc
     1621 tcacgagagg tccggcttca caaagtccca tacaaccagt tttaaccagg tgaggacttt
     1681 cttctaattt ctccacttct gcttctactt tttctctttc tatcactttc ctgaaggtat
     1741 caagtacttc ttctgctcct ttagctaagc accctaaccc tccacaaatc aggactttct
     1801 tctttccgtt tttgtttttt aattcaataa tatatttttc taattgttcc cgattttcaa
     1861 accgcatcta ctttctctcc ttcccctaac aggtgatcaa caagctgctg cactttttct
     1921 tcgtccattt gaccataaac ttcttcatct accataagag ccggagccag agcacaagcc
     1981 ccaatacaag ccaccgtctc cacagtgaac atcaaatctg aggtggtgtt ttctccttct
     2041 ctcaatccca aattttctcg aactgccttc aaaatttgcg cagaaccctt tacatgacac
     2101 gcagtaccgt cacaaaccct gataatatgt tcacctttgg gctccagaga aaactgagca
     2161 taaaaagtag ccaccccata gacgtgcgct ggagaaacct gtagagcagt agcaacatag
     2221 gtgagaattt cttctggcag atagcgatat tccgactgaa tctcctgtag tatggcaatc
     2281 aaattgtcca cattatgaga atattttctc agaatagcat tgaccttttc aaactgacga
     2341 ctgagaacta agctttcttt ttctatttcc atcactctac cccctttata gataacatta
     2401 gttttttagt aatattccag atttactatt ttccctcttt tttgtaactc actaactata
     2461 tcctctaaca accgagcttt tagcgaaagc tctccagaga gtttaggatt ttggtgagca
     2521 ggattgatag ctcttcctcc tataaaggta atctcatccg cttctaggag aagtgagagt
     2581 aaacggctag ccccatctcg ctggtaaata acttcttcta actccactcc tttccgcagt
     2641 cggcgaagaa ctgtggccag agtcaggatc ccttcagtaa gtaaatccac tccttccatc
     2701 attcctaccg gaggaatatc tttttggaga gaagttaaat ctacttctat ctctttgccc
     2761 aaaacccggg ctaaaatgtt tcctgtagta ccaccacaca ctgctttctt acccggaaat
     2821 tccattaccc ttttgactat atattcatct aaagccgggt cttcaggagg cccggcccac
     2881 actattaagt aacgaggaag acgtatttta gctaccacac aggtagcatc atcacctaat
     2941 ctatcctgat aaagcctttt agtagtagct atcactttat tagctagagt caaagcatcc
     3001 agctggtcat taacagtatt ttctaaaaaa gaggcgattt tttcccatct ccatcccaaa
     3061 ttccacaagc ctccaatccc agcatgaacc actccatcgg ttactcctac aatccaatca
     3121 tttttttgca ccctcattat agattctaaa accaccctct ctcctatttc tctttctttt
     3181 ctatccaggt gcatcaacct tccattacgc aaccagaaaa gagggggatt atcaaactct
     3241 gctacatagg tctctccact ggagagaacc tgtactacag aaaaagtact ataggcaatt
     3301 ttcctcacct tacacaccgg taaggtttct gctaaagtgg ctatcacttc ttccaaagta
     3361 gattctcttt tcaacatgga aacaataatt tttgaagtca gagtggaaag aatattagcc
     3421 tttactccac tacccaaacc atcagaccag gccagtatgg tagaattatt tcccttatca
     3481 taactcacac tatctccaca aagctcttcc ccccatttgg gaagctgagc ataggcaaac
     3541 tctacaaaga ggtctttcat ccctctaccg attctccccg gagtattttc atcaacttat
     3601 ctagaagaac tttagtttca gcagtagttt cccctaataa gctggcaatt tcctgagcta
     3661 ctctcatctg gttattaatg acctgttgag ctttctctaa agtttcttct ttgatttgct
     3721 ccatcaaaac ttctctttcc ttttctttgg tcaaatccac aaataccccc ataagaagct
     3781 gagacttctc cagataaaaa acagacaatt gaacgtaaag gttctggtat tgaggataaa
     3841 acacctctct ggtgaccatc tttttagtgc gcaacgcctc cacaaaagga gatggatcag
     3901 taagctggtc taatttcccc cccaccacta acttccccgt caaaccgaac atcctacgaa
     3961 gagccgggtt aatatccaca atgcgcagat tttcatcaac cagcacaata ccattgggag
     4021 ttactgcaat caaaaagctg gaaaaagatt ctgctcttgc ccgcataaaa ggaatacaca
     4081 tctccgcttc cgccatgccc tgataaaccg cttgagcttt ttctcgacag gtgttatacc
     4141 cacaagcacc acaatttagc tcatcttgag gagaaaattt actggtaaga gataaaatcc
     4201 tttttatctc ttcttccgga acttctggct gaggtatggg atgaggtgca aagtttcgac
     4261 taaaagtggg aacagaaata atttttttct ctggtggagg attagaagcc atcttagctt
     4321 tttcggtata gtcaatgact tttttccgcc gttcgtaagg agaaagcgaa gagcggagca
     4381 ccggtccatc aatgcagcct ccctcacagg acatcatttc caccatttca aaatcacctt
     4441 tggtgtcttt taaatcatct aaaaactgac gacaacggtc gattccagtt attaccataa
     4501 cttctttgga caaaatcccg gtatccaaag aagcagtttt caggagtcct ccttccacgg
     4561 ggaaagctct tccccactcc actgaagata aacccatttc ttcttcctct aacgcagaaa
     4621 gagagatatt ctcttcttga aaccaggttt ttaattcttc gaaagttaaa actgcatcta
     4681 cttctccttt tacttcttcc tcttcaattt ctgctttttt ggcaatgcaa ggaccgataa
     4741 agaccacttt tactttttca tactcctgac ggattaaacg agcatgagca accataggag
     4801 agacaactgg agcaaggtaa gaaacatatt gaggataata cttataaatc aaagcattga
     4861 tagctggaca ggaactagaa ataatatttc ttttcccctc atctaaaagc cgggcatgct
     4921 ctctggctac ccactctgcc ccttccgctg tttctcgtac ttcttgaaaa cccagttttt
     4981 tcaaagccgc cactatctgt ccttctgtga cctcagaaaa agcggctaca aaagaaggag
     5041 ctaaactgac cactaccggg tcttctccct ggagcagttc ttgaacccgg ggaaggtcat
     5101 tttgaatttt tttagcatct tgagggcaaa ccagaacaca gcgtccatct tttatgcatc
     5161 tttcatccac aacttcagcg tgtcctaaac ttacccgaat ggccttcacc ggacaattgc
     5221 gtatacattt atagcaatcc cggcatcttg cctcaatggt gctaattact cccatcagta
     5281 ccatcttctc ccagcttttt caaaatcttc tcctggaaaa tctccctgaa attctgagga
     5341 gaaaccccac tgagtatctc atcatccacc attacagtaa ccccctcttc tgtgcatctt
     5401 cccagacaaa agttaccttt taattctatc tgatctttaa gctttaattt ttcaatcaac
     5461 tcttcacaaa tttcaataat ctcataagcc cccttaagat gacaagaact acctacacaa
     5521 accgatatag taaccatcct tacctctcct aaaaaaaaga atataccact ttatctttaa
     5581 gataaccaaa aacatcgaac ataaaaacag agggagtgaa atcacctccc tcagttttta
     5641 tgtttttcat aataaattaa tcaaattata ttttagctgg ttattataac ataaatttag
     5701 atttctaaaa cccacacttc cagattagtc gggagaatct ctcgagtaac ttctacctca
     5761 gaactaccca acctcttcac ctccctaaaa ccctgcagaa aaggttccag aggattaagt
     5821 tcaaatttag tatattcagt tcggtagtgc atcggtatta caagatgagg agaaagcttg
     5881 gaaaccaatt cttgagcttc ttgagcatca atagtgaaaa tccctcctac cggaagcaat
     5941 aaaatatcta ccggtttcca ggaagatagc tcctcatctg gaggtattat tcctaaatct
     6001 ccagcgtgaa tgagagacat cccctccaat tccaaccggg caacattatt tttaccccgt
     6061 ttctttcccc ctacctcatc atgaaaagta gagaaaattt ctaccttaat cccatttttt
     6121 tcataaaccc ctggttcttt aattatctca gggtgtcccc gcaccagaga aacgttattg
     6181 tggtcaaaat ggtcatgact tactacaact aaatctgcct ctacttgagg gaggggataa
     6241 ccaacactat catcaaaagg gtcaaaggct attttcgtgt ctccactcgt cactaaaaag
     6301 aaagaatgtc caaaccaacg aattttcaac ttcctcacct cctgcattta ttctactata
     6361 atttactata aaatagataa ccaaaaccct tgtactttct ttccctgctt tagaaaagag
     6421 aactataaga acccaggtta aggatagtcc ggtgacgggg tttaccacct actctataac
     6481 tctcataata attctagaac tacaaagggt ttttagataa cctactaaga agaaaggctg
     6541 ataaatcaag gatctgtacc tcaagtatac ctgaaaatag gagatttttc tgggaaaaag
     6601 ccgcaagttg gatgaagctt tttatgatgt cttatcaaga gcctggcaat aagaagaaag
     6661 aagacggcga aatcctcgtt ggggacttct ttcttcttga ataatgacct gcaaaagagc
     6721 agagcctatt attaccccat cagcgatttg agccaatttt tgagcttttt ctggtgagtt
     6781 cactccaaaa ccgataagca ctaaagaaga agatacttct tttataagct ctactacttc
     6841 ccgcaggcgc tcttcctcca tctccacctg accggtcgta cctttaccgg aaaccagata
     6901 aacaaaaccc tcgctctctc tggcaatctg ttttatcctc gccggggaag aagtaacaga
     6961 agcaagtagt acccgggata agtatccttc catttctttt tcaaaatgtc gtgcctccca
     7021 gggaaaatca gcaatgatta accctgaaaa cccggcatct ttaaaatctt gagcaaagcg
     7081 ggaaaatcca tactggtaaa cgatgttgcc ataagtcata gctatcaaag gtaaggcagt
     7141 tctttctttt cgcaaagttt ccacaaccag aggaggggta actcctttct ctaaggctct
     7201 ctgagaagta gtttgaatta cagggccatc ggcaacaggg tcagagtgag gaatacccac
     7261 ttccagcgca tccactcctt cttcttcgca aatttgtatc aaatccagaa aagtaggaat
     7321 atcaggataa ccaaaagtaa gataaggaat gaggagcttc ccttttttcc ttctctcttc
     7381 taatttttct tttaatatgt cagccatttc tcttttcctc ctctaagtag cgtagaacct
     7441 cttctacatc tttgtctcct cgcccagaga gacatatcac cataactttc cccggaagat
     7501 gaggtgctat tttagaagca taagctacgg catgagcact ctccagagcc ggtataattc
     7561 cctctttttt agaaagttct aaaaaggcct gcagggcttc ttcatcagta actgatacat
     7621 actgggctcg accggaagag aaaagatagc tatgctctgg ccccaccccg gggtagtcaa
     7681 gaccagcaga tacggagtga gtttcttgaa tctgcccaaa ctcatcctgt aatacatagc
     7741 tcaagcttcc atgaagaatt ccctttcttc ctcctccaat actggcagca tgttttccgg
     7801 tctctattcc ccgtcctcct gcttctacac ctatgagagc tacattatcg ggaaaaaatt
     7861 cataaaacat tcccatagca ttgcttcctc cccccacaca agctaaagca tagtcgggga
     7921 gtctcccttc tttttctact atctgctttt tagtttctct accaattacc gattgaaaat
     7981 cacgcaccat ttgaggataa ggatgaggtc ctacaacaga gccaataaca tagtgagtag
     8041 tatctaagct tcttacccag tctctcatag cttcgtttat ggcatctttc aaggtctgac
     8101 ttccactata aactggaatc acctctgctc ccaaaatctt catacgaaag gcgttgagtt
     8161 tttgtctttc tacgtccttt gctcccatat acacctgaca ttccaagccc aaaagagcgg
     8221 caacggtagc tgtagccact ccgtgttgtc ccgctccggt ttcggcaata attctctttt
     8281 tacccatttt tttagccagt aatgcctggc ccagagtatt gtttatttta tgagagccag
     8341 tatggtttaa gtcttctctt ttcaaataaa cccgcgctcc tttccaggat tcggttaagc
     8401 gctctgccag gtaaagagga gtaggccttc cagcataatt tttcaggtaa taatttaatt
     8461 cctcctggaa tttttcatcc tttcggtaat attcgtaagc ttcctctaac tcctctaagg
     8521 ggtgaattaa aatttcggga acgaacttcc ccccaaattc tccaaaaaat cctgcctttt
     8581 ccattttatt ttcctcctat cttttctaaa attcttttta ctttttcagg atcctttttc
     8641 ccggggtaaa tttccactcc gctattcaag tccaaagcaa aaggatgcag aagagaaaga
     8701 cactgccaga tattttcctc ttttaagcct cctccgatta ttattggttt gggaaaactg
     8761 atccctttta tcttctccca ggggaaagtt atcccgcttc ctgctttttt gctatcgaaa
     8821 agcaggaaat ccactactgg aaggtattct tctaccatag cgacatcaaa atcttctccc
     8881 acagaaaaag ctttaatcac tcctttcccc aatttttcgc aaaaaacagg gctttcttca
     8941 ccatgtaatt gtattaagtc aaaactgcac aactctcgta atttattcac ttcctctacg
     9001 ctttgattct ggaaaacccc tactttcatt atccgagaag gtagagagtt aactacctct
     9061 ttcgctttct cgggaagaat aaaacggggg ctatcctcca ccaaaataaa gcctaaggcc
     9121 cagataggaa attcacccag aagtaaaaca tctctttttt ctttaactcc acaaacctta
     9181 acccggggca acttccactc cccctctaag ttcccttaat ttctcagctg ggtggcagga
     9241 agtaatcaag gtctctccta tcaaaaaatt gtttatccct ttatccatta atctttttat
     9301 atcatttcta cttttaattc cactttctgc caccactatc accgagggag ggatttttcg
     9361 acaaagtcgc tctgaaactt gaagagaaac ctgaaaagtg gccagattac gattgttgat
     9421 gccaattaaa ggagcgtgag cctgtagtgc tttttctaca tcttcctcat catggacttc
     9481 cacccagggg aggagatgca aaagagtgca gaggtaaaca aagcgttgca gtcgttcccg
     9541 ggaaagaaga ccagcaatta aaaggagagc gtctgctcca ttttgcgctg attcataaat
     9601 ctgggtttct tctattacga aatctttacg gagaagagga agaggggtaa tttttctcac
     9661 ttcccgaaac atctccagac tcccttgaaa ataatcttct tcagtcacca ctgatattgc
     9721 taccgcctct ccttcttcgt attcttctaa gaaaggaagg aaacttcctt ctatcaacac
     9781 cccccgagaa ggagaagcct tcttaatctc agctatgatg ttaggttcgg gtgaattcct
     9841 caaggaggag agaagagtat ttttcttctt ctctcgctca ctaaatacat ctaccacttc
     9901 ccgggaagaa tattgcttcc cttccagaag cctctttttc tttcctacta ctattttttc
     9961 cagaaacccc tccatcattc tccctccagg gctctgctta cctttaccac tcttttcaac
    10021 gtttgcagcg ctcttccaga gtggattgct tcttctacct tttctatagc ctcttctaaa
    10081 tcttgagctt cttctcctat ccagagcaaa aaagcgctat ttaaaactac tgcttctctg
    10141 cgtgctcctt tttcttctcc tcccaaaata ttcacaaaaa tccgggcgtt ttcagcaggt
    10201 gttcctcctc ttagctcctc tatatttctt actggaaagc ctgcttcttc gggatgaaaa
    10261 gaggaaaaag ttattttacc tccttctaca aaagctaact tactctctcc agtggtactt
    10321 atctcatcta aacctggctc tccccacact accaaacctc ttttcacccc aagagtagcc
    10381 agggttgaag caacaggctc aataagttcc ggagaataaa cacccaccag cttataatcc
    10441 atggggcaag gattggtaag aggtcctaag aggttgaaaa cggttctaat tcccagttct
    10501 tttctcactc cttgcactgt ccgcgtagca ggatggtaga gcggtgcata aagaaaggca
    10561 aatcctgttt cttcaaaaag agtttgcatt ttttctcttt ccatatctac ctttatcccc
    10621 aggatttcca gtaaatctgc actaccacag cggctggaaa ctgaacgatt accgtgtttt
    10681 actattttta gcccggaaga taaagctaca aaagcggaag cagtagagat gttgaaggtt
    10741 ccctgtccat ctcctcctgt cccacaatta tcagccacag aaagggaaga aactgaaaaa
    10801 ttcattgctt tttctctcat cgcttctgca aatccggcaa tttcttctcc atcttctttg
    10861 cgcatccgca gagaagatag gagtgctccc atctgggaag aagtaaactc tccttccatc
    10921 aagcccctca tcagagaata ggaatccttc ctggtgagcc ttttaccttc tattatgtac
    10981 tctaagaaat ctcgagcggt ttttaacatg agagtcccac ctccagaaaa ttctttaaaa
    11041 ttgtttttcc ctgaggagtc agaactgatt cagggtgaaa ttgcacccca aaaagaaggc
    11101 gagaaatatg ctgcaaagcc attatttctc cactttctgt ttgggcgatg acctctaagg
    11161 aaggtggtaa actttctcgc tcgacaatga gagagtgata tctacccacc cagatccggg
    11221 gaggtaatcc tcgaaaaaga ggagagggaa aaagataaac taaagacttt ttcccgtggt
    11281 aaggtcgagg ggcttttact acctttcccc cttcagccca ggcaacacac tgatggccaa
    11341 agacacaccc ctaaagtggg gatagaggga aaacaggaaa ttatctcatt gctaattccc
    11401 gcctcctgtg gctctccaga gccaggagag ataacaatct ttgccggttt catttgctcc
    11461 acttctgaga tagaaatttc atcgttacgg aatactttga tttcttctgc agttagctct
    11521 cctaaatatt gcaccaggtt ataggtgaaa gaatcgtaat tgtcaatcaa gagaatcatt
    11581 aatcattccc ccttccggct aaatttaaag ctaaaagtaa agcctcggct ttacttcgtg
    11641 tttcctcata ttctttctga gggagagaat cataaacaat ccctgccccc gcctgaactt
    11701 ccgcttttcc ttcccggaaa aagatgctgc ggataatgat gcaggtatcg aggttgccat
    11761 ttaaactcac atatcctaac gctccagcat aaggcccccg ggggagaggt tccaactctt
    11821 cgattatctc catagctctc accttaggcg ccccactaac tgttcctgca ggaaaagatg
    11881 cctgtagtgc cctccaggga gaaatgcaag gggctaacct accagaaaca gtagatacga
    11941 tatgaaaaac atgggaatag cgttctatat ccataaattt ttccacctgt actgttcctc
    12001 tatcacatac tctccccaga tcacttcttc ccaggtctaa gagcatcaca tgttcggcaa
    12061 tttctttctc gtcattaagt aagtctccta ttatttcttc ctcaggaatg gaagacagtc
    12121 tctttctcgt tccagcaatg ggtttggtga aaattttccc attctctatt tttatgagca
    12181 tctccggaga agatccagcc atctgaaact ggtcagtctc cagataaaaa agataaggag
    12241 aagggttcaa cgagcgcaaa aaacgatagg ggataaaagg gtcgccagaa aaagcttgag
    12301 aaaagcgctg ggaaataact acctgggaag catgtccttc ccggataaag tctctcactt
    12361 tttctacttt ttctctaaat tcttcctcac taagagcaca ctccacctct cctttagttg
    12421 caaaaaagga ggagggagag gatgattgac ctattctctc ataagtttct tctatccact
    12481 ttttcccctg gttataatct tcttcttcgc ctccccgggc aacatatact gctcccaaaa
    12541 gatgtttgag gtggtcaaaa tagagaaaac ggttgctttt tatccagtag gctaatggat
    12601 agcttatctt ccggcgagaa atagttttct cccaggtttt cactgcatca taactcagaa
    12661 aacccactgc tccccccaga aaaggaaggg aaggttcagg ataaacggag taagaagcaa
    12721 attctctctc cagaagggaa ataatgtcag taaagtcttc aatttttacc tcttctcccc
    12781 ctcggattaa aaaagaataa cgcccccatt tttcccctct ttctgcactc tccagtaaaa
    12841 tcaggggagt ttctccccga aatttgtcta ctaacgatac cggagtaagt cggtcggcca
    12901 gctgctcggc ataaagggga ataaaatcat aaccctcagc cgacaacttt ttaaattttt
    12961 ccctatctgg ttttatctcc atgttttcct cccataaaaa aagccctcgt caagagacga
    13021 gggcttaaac tcgcggtacc actctcatta acggcgttaa ccgttcactc tttcggtaca
    13081 aaaactacta tacctacttt ctttagcggg aaagaaccgt ctcagtctac tcgagtattc
    13141 cctttcgatg agctgctccg aggcccattc acctttctct ccaacaccag gcttccacct
    13201 accctggctc tctgtagttg aaggaaaaaa gctactctcc ctcatcatcg cttttcttat
    13261 tggcgcttat taaagcacaa ctttttctct tcgtcaagag gtaacgggct ttatatagtt
    13321 ttgggttaat ctggagatga tgagtactta tgaaaacatg tactgttgtc attcagtaac
    13381 atccagtaaa agggatgata ttttatgaga ataaaactac cccccttcag gcgagatttt
    13441 aggagaatat tgacataaat tcacctgctc atctttctgt gtgataccaa gaaccaggat
    13501 aatatttagt ggaaaagagt tcttctgctt tttctaactc ttcaacactc cactctcctt
    13561 cttctatctc tacgccccac tcttctctta aactttctaa aagtaaccgg taaattttcc
    13621 ttttctcggg taaaggagaa agaacttcac agagggcaag aaggctattt tctaattctt
    13681 tctgaatttt ttcttttttc ttaaagcagg aagcaacttc ttctctgttc ccctctaaaa
    13741 taaaggaagc ttgaaagaga tttcccctct ctcctcgagc ttgagccact ccaataattt
    13801 tccttcctcc gatcgttatc tcatgagcaa cattgataga aaaacaataa ggggaagaag
    13861 tataaatccc tgattctgtt ttttcgtcta cttttattcc catcttttcc atcatccggg
    13921 tcagtacttt ttgcaattcc ttataataaa aagtatgttt ctcaaaagaa gaagtgggaa
    13981 taaagagact gaaagtaacc tccttatcgt gcaaaatagc tcgtcccccg gtaggtcggc
    14041 gcactaaagg aatattttta gcttcaagat agctaaaatc catttcggca gttttctgga
    14101 atctccccac cgagagagtt tcttcctccc actcaaaaaa acgcaaagta aggggagcat
    14161 cgggaaaacc catctccagg agtgcttcat ctctgctcat attttcatag ccagagaggg
    14221 gaggatcaac gagaactcta accacttttt tcattgagtt ttcaaaaata cttcgtactc
    14281 ctctttattc atcaaattct taaattcttc ttcgttttcc ggctggattt tacaaatcca
    14341 gccctctccg tagggactct gattaattaa ctcgggtttt ttgtccaact ctctgtttac
    14401 ttccattact tctccagaaa ggggagcata taaataagaa actgttttta tcgattctac
    14461 ttcccctatt ttatctcctt gctttacttt cttaccctct tcgggcagtt ctacaaatat
    14521 tacatctccc agctcatttt gagcgtaatc agtaatgcca caagtagcta aatctccttt
    14581 ttttacccag aaatgttccc gggtataaag taaattatcg ggaatattca catttattaa
    14641 cctccttccc caaaaggtaa attcactatt attgcttcca gtcgagtccg gggtgcagct
    14701 accagcaaag tctccccttt ttcaccggag atagagctaa taaaccccag agctaaaact
    14761 ttatgccagg aaaaagaata gttaccactg gttacataac cgcagggcat cccttcttta
    14821 gtgtagaggg ggtagccata acgaggtatt cttcgcccca gaatctctaa ccccaccaat
    14881 ctctttttag aaccttcttc tttctccaga agaagaggtt ttttaccaat aaaatcggct
    14941 ttctcccagt ctacaagatc ctctctcccc agctcaagag gagtaatggt ctcatctatc
    15001 tcctgaccat aaagagggaa ggtagcttca aaacgcaaaa ggtctcttgc ccccaaccca
    15061 cacagcgcac cccctcggcg ctctacctct ttaaacaatt tttcccagag tcctgaaccc
    15121 agagagacat cactcattat ctcaaagcca tcttctttag aaaaggcaag atgtgttact
    15181 tgaaatccgg gaggaaactt tcgaaaagaa aactttggcc aattatcacc ggaaacctga
    15241 aataactcct tcgctattac tgccgcttca ggaccctgta tggcaaaaag cgctttcttt
    15301 tccgttacgt cttctatctc tacttcccat ttattagtct ttatcagata atcgagccac
    15361 cggaaaagct tttcccgcgc cccggcattg gaaatcaaaa gccattcttc ttctttctct
    15421 ctaaagagag ttgccccatc tactattcct ccccgccagt tgagaaaaaa aatgtactta
    15481 cctcttcctt ccttaatttc agaaactttg cgacaggtag cataatctaa aaaaggagtt
    15541 gcctctttcc ctcgaagaag aaacttcccc aggtgagaaa tatcaaagag tccacagcta
    15601 ttagtacagg caaaatattc ctctcggata cttcggtaat aaaggggcat ctcccaacca
    15661 caaaacaccc ccattcgagc accacaagag cggtgaaaat cagaaagagg tagctctcta
    15721 agtgtcatct atcctccctt taagaaaaat gtaagggaga ggcaaaaact ccttgagctg
    15781 cttccattaa agcctcagag agagtgggat gaggatgaat agtctcggct atttcttgcg
    15841 gagtgcattc taagtttaga gctaaaactg cttctccaat caaatctgaa gcgtggggcc
    15901 ctaaaatatg tactcccagt atctctccat ttttagcttc actaacaatt tttaccagac
    15961 cctccgtttc cccctcaatc agagccttac cgtttgctcg aaaggggaat ctccccactt
    16021 taacttcata tccttttgct tctgcttctt tctcggttaa tcctacagag gcgatttctg
    16081 gaaaagaaaa aatgcaattg ggtattgcct tatagcttat tccctttttt tctcctttaa
    16141 tgtggtttat cgctacctct gcctctctgt aggccacgtg agctaaaaga tatttaccat
    16201 ttacatctcc cggggcatag atgtgagaga tagaggtgcg caaaaactca tcggtccaga
    16261 ctccccgatt ttccattttt aaacccagat tttctactcc taaattttct acctggggca
    16321 ctcttcctgt agccactaaa acacaatctc cttctacctc tttttcttct ccttcttgaa
    16381 taaaggttac tcgcaggttt cctgagcgat gaatcttttt cacctgagca ttaacatgaa
    16441 ctctcatccc tcttttttct acgagctgag tgagaaggtt ggttatctcc tcatccaccg
    16501 gaggaagaat cttgggcatc atctccacta cttctacctt gactccaaaa gcattaaaaa
    16561 tgcaggccat ctccatccct ataactcctc ctccgacaac taccagactc tcaggaagtg
    16621 aagaaagttc cagtgcctgg tcactatcga ttaccccctc taaattagct ccttctaccg
    16681 ggagagtggc agagcgggaa ccactggcca gaactatata ttttccccgg atttcttctt
    16741 tttctccccc ttctttttct acccttatag tatgagaatc cagaaaagaa gcaaaaccct
    16801 ctattacctc cacttttccc ttccggagga gaaattgtac tccactggtc agctgtttta
    16861 ctacccgatt ttttcgattc aggacctttt cccaatcaac tccttgaaaa tccgctttta
    16921 tcccaaaatc ctgtgccttt tttaccagat tagctacttc ctgagattta aaaaagacct
    16981 tggtaggaat acacccccgg ttaagacacg ttccacccaa ctctcttcct tcgacaagga
    17041 tagtttccca tccctctcgc cctgctcgag tggctgcagc ataacctgct ggacctcctc
    17101 caataaccac aacttctgct tctttcattt tattgcctcc attcaggaaa actattagag
    17161 tcggtaaaaa aacggtctat agcttgcgct gaccttttac ctgcacccat agccgatatc
    17221 acagtagccg ccccagttac tatatccccg ccggcgaaaa ctcccgctaa actagtagct
    17281 ccggtttctg catcagcggc gatgtaacct cttctattca attgtaagcc aggaatagtc
    17341 tctaagagca gagggttagg accctgtcct atagccacca ccacagtgtc aataggaata
    17401 acaaattctg aaccaggaat gggaacaggc ctccttcttc ccgactcatc cggctctccc
    17461 aattccattt gaatacactc aatacccgtt acccaaccat tttcattgcc taaaaaacga
    17521 accgggttag tcaaaatgat gcagttcact ccctcttctt ctgctctttc tacttcttca
    17581 attcgagcag gcatctcctt tttagtacgt cgatatacca ggcacacctc ttttgctccc
    17641 aaacgtaagg cggttcgtgc agaatccata gccacattcc ctcctccaat cactgctacg
    17701 ctttgcccca ctttcaaagg agtgtcataa cgagggaaaa ggtaagcttt catcaggttg
    17761 gaacgggtga gaaattcgtt ggcagaataa ataccattca agttttcccc gggaacttcc
    17821 aaaaagtaag gaagacccgc tcctgttcca ataaagaaag cctgataccc ttcatctttt
    17881 aagtcatcca gagtaaaagt tttacctatt acccggctta cttttaactc tacccccaaa
    17941 gactgtacat aatcgacttc ttgcgccaca atagccttgg gaaggcgaaa ttcaggaatg
    18001 ccgtaaacta aaaccccacc tggcgcatgc agagcttcaa aaatggtaac tttatagccc
    18061 attctggcta aatctccagc acaggttaaa ccagccggac ccgagccaat caccgccact
    18121 ttcttatccc gaggaggagg aagagaagga gctttcactc ccttttctct ttcataatca
    18181 gcta
//
LOCUS       DAOPSS010000018        18157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2597_length_18157_cov_8.885758, whole genome shotgun sequence.
ACCESSION   DAOPSS010000018 DAOPSS010000000
VERSION     DAOPSS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 18157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18157
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2597_length_18157_cov_8.885758"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PKN31_02180"
     CDS             <1..1271
                     /locus_tag="PKN31_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOA98771.1"
                     /translation="RRRKREDSSISLPIKKTDGAVAGVLNLNRTDCPFEEEDIPQLEA
                     LTINIAIILEENSLRRRREHIIVALSEIVGLFSSLFCTDTSSDVFEKIFYSVKTLTGA
                     RKAAVFKLSKKRPFLVFEKDWPKQLRWKDFAEVDDQIQECIEQKEVITIEHSSRIFFL
                     FIPLVSGNHPPYLFTGILKKPITIIDYLVLSIVVNMGNAALENISLYQKSRRLAQERE
                     RNRLARELHDGLTQIMVTTQIYLHLLEKSLENADSNDLLILQKVQSLNKLGLEESRFI
                     LSELKGKPITPLQFREKVDETINLFATPDKEVEFDYKVDVKEIPYQVYQTVLKILQET
                     LSNIQKHAQAQKVKICIMNSKQQMIFSIEDDGVGFDPEALEEKESEHFGLRSLKERVR
                     VLRGKFDIDSQPGWGTKIMVKVPLPREIAV"
     gene            1288..1935
                     /locus_tag="PKN31_02185"
     CDS             1288..1935
                     /locus_tag="PKN31_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA98772.1"
                     /translation="MKIRVALIDDHPIFIAGLKQLLESDGRYEVKAVAQSGEEARKSI
                     DFNEVDVALVDVNMPGGSGIELIRFIKRQKGDCRVIMLTVEEDEETINRAIKEGAQGY
                     ILKQESPEHLLKAIQASMDGEILLSNHVYTRMVDRIRKTSPLDKEQSILYNKLTEREI
                     EVTRLIVQGKNNKQIAQELYISESTVKNHISNIMDKLSIKSRTELVTIAVREGIQ"
     gene            2007..2447
                     /locus_tag="PKN31_02190"
     CDS             2007..2447
                     /locus_tag="PKN31_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA98773.1"
                     /translation="MFSIYSSIQDLELQSLEDGKLTTEVKISQKKVFVVDDNRFFLES
                     LVFLLNRQKDIKVVGTYHRARGVLQKIKAAQPDVVILDVQLPDGDGISILQEIKSQLG
                     IPVIMLTIYEEGRERAMKNGAYAYLVKGEELDILYQKIRQIGEI"
     gene            2824..4377
                     /locus_tag="PKN31_02195"
     CDS             2824..4377
                     /locus_tag="PKN31_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA98774.1"
                     /translation="MAVVEKKEKNSKEGKTVEVAFRSITKRFPPDILANDRVSFEVEK
                     GTVHALLGENGAGKTTLMNILYGLFPPDEGEIIIRGMPTKISSPREAINLGIGMVHQH
                     FKLISTHTVSENIILGLASTPFFFPAEKAESEIRFLSERYGLKIDPRARIWQLSAGEK
                     QKVEILKALYRRAEILVLDEPTSVLTPQEAEELFATLRLMKEEGKTCIFITHKLNEVK
                     EIADAVTVMRRGKVISTILPSSVSLEELARMTVGKEVISVVPRKTVSGKIILQLQKVS
                     AMGDREIPALSNLSLTVREGEIVGVAGVAGNGQKELAEVIVGLRQATEGHVFLQDKEI
                     TNLPPRDIASLGVAYIPEERHMGLVGEMSVAENLLLRGYSRPPFSQGFWVDWEKAEAY
                     SEKLISNYHINPPIADIPVRLLSGGNRQRVIIARELERKPKLVIASNPTAGLDVSGVE
                     AIHELLVKAQEEGAGILLISGDLDELLKLSNRIVIMFKGEIVGEKEKEHWTEEDRREI
                     GLGMGGVRI"
     gene            4378..5484
                     /locus_tag="PKN31_02200"
     CDS             4378..5484
                     /locus_tag="PKN31_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA98775.1"
                     /translation="MGRRKLTSFRVELSLQPTLYSVIFFSLLAIVLAFACGSIIFLFY
                     HISPWIAYQTFFRGAFGSWYAFSETLKRTIPLLLVGEGLALAFRAQVLNIGAEGQILL
                     GAVAATWVALFSGLPSFLLLPTMFLLGFLAGAGWALIPAFFKTRFAVNEVLSTLMLNY
                     VASNLVLYLIGGPWKGKGVRGFTYTDTFPEAAWLKTMGITRVPYFTLLTGIVLAVGGY
                     IFLKKSALGFEFRIVGNSSETAKLLGISEEKIVLFAMFLSGGLAGFAGVGEVAGIHHL
                     LRHPDHISLGYGYTAIIVAWLARANPLATIFTSFLFGALLSGGDALRVSLGIPFQVVS
                     VFNGLILFFLISTERLSRYRIIWGEEKAVERSGS"
     gene            5474..6403
                     /locus_tag="PKN31_02205"
     CDS             5474..6403
                     /locus_tag="PKN31_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA98776.1"
                     /translation="MALELVLGTLNRALAYGTPLLLATLGEIYVERAGVLNLGIEGMM
                     MLGAFSSFCVAYLTSNPWLGILTGAIAGGLFSLIHAFSSVTLRANQTVSGLALTMLGT
                     GIAETLGRSWEGKVLTSPIRPFSEDFLSLFPLWMRTLFRDSNLLVVGSIILALVLWFF
                     LFKTKPGLFLRSCGENPAACDALGVNVWKVRYIAVFWGGIMAGLGGAYIAVAYRPTWS
                     SGMTQGMGWLAVALTIFSFWNPLYAPLGAYLFGALFHLAFRLQVFVAPEILNSLPYLF
                     PVVALVFFSRLAFRKKIGVPGSLGKPYERGESE"
     gene            6434..8482
                     /gene="tkt"
                     /locus_tag="PKN31_02210"
     CDS             6434..8482
                     /gene="tkt"
                     /locus_tag="PKN31_02210"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOA98777.1"
                     /translation="MEASVDQLTVKTIRMLALDTIQKAKSGHPGLPLGAAPMGYVLFR
                     RVMKHNPKNPRWFDRDRFILSAGHGSALLYTLLYLSGYEKMSLDELKSFRQWGSHTPG
                     HPERCIDCGIEATTGPLGQGFAMGVGAAIAEAHLAARFNRPGFPIINHYTYALVSDGD
                     LMEGVAQEAASLAGHLKLGKLIYLFDNNRVSLSGETSICFTENIRMHFEAYGWQVLEV
                     KDGNNLEELVRAIQEAQKDESRPSLIMVSTHIGYGSPKQDSFEVHGSPLKPEEVVETR
                     KFFGWPLDQDFYVPEEALNHMREIIREGEEKEKEWNNLFSSYAREFPELAQELTMLIK
                     GELPPRWDEGLPVFDPASGDIATRSAGGKVLNALADKIPSLIGGSADLDPSTNTVLKG
                     KGSFQAPGSCPPGTQGLVEGPLGYEGRNIAFGVREHAMGAILNGIAIHGGLIPFGATF
                     FVFSDYMRPATRVAALSHCKVIYVWTHDSVALGEDGPTHQPVEQLMSFRAMPNLVCIR
                     PADANETREAWKIAIERKEEPVALILTRQNVPVIDRDKYGKAEGLRKGAYIIADCAPH
                     LPQLIIIATGSEVSLALEAYEKLSSEGVRVRVVSMPSCELFEEQPEAYRREVLPPEVK
                     KRIAIEAGATLGWYKYVGEEGKVIGIDRFGASAPGKVVLEKLGISLENLLAQARSLLE
                     "
     gene            8964..10130
                     /locus_tag="PKN31_02215"
     CDS             8964..10130
                     /locus_tag="PKN31_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA98778.1"
                     /translation="MNKKAVLAVVLLLSLLITGVAFSEEAKLKAGFIYVGPIGDLGWS
                     HAHEQARQVLEEVYPWLDTIYVEAVPEGEVEGIIDRLINQEKVDIVVTTSFGFMDGTL
                     NAAKRYPDKIFFHCSGFKRAPNLATYMADFHQVYYLNGIMAGALTKSGKIGYVGTFPT
                     PELKRHINAFTLGVRRVNPQAEVNVRWLQSAWYDPQGAKEAAEALIAEGIDVLAFTED
                     SASVAQVAEEKGLPSFGHYSPMLSYAPNHLVSGQLVHWEAIYLDFFAKVYAGLYNNEN
                     LENVDYWWLLSNGAVELGADFGVPVNPAFEEALKSYMVNDPVYGEMSAYDLVFELEKA
                     MADPEYAFSPFTGPIKDRKGNLLVPSSVRLALEDLITMEWAAEGVAGPWPGEPE"
     gene            10241..10315
                     /locus_tag="PKN31_02220"
     tRNA            10241..10315
                     /locus_tag="PKN31_02220"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10273..10275,aa:Gln,seq:ttg)
     gene            10483..11871
                     /gene="glmU"
                     /locus_tag="PKN31_02225"
     CDS             10483..11871
                     /gene="glmU"
                     /locus_tag="PKN31_02225"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOA98779.1"
                     /translation="MDLSQWGLIILAAGQGKRMRSSLPKVLLPAWGRPIMQYLLDELK
                     WEEFGQRFLVVSPSMEELIKSSFGQDITIVVQKEPKGTAHAASTVFPLMQEGIDWFLI
                     LYADMPLLQGETIFSFLSRVEENRYPFSFLTSFSPDPAGFGRVVRDEKGEPLGIVEEK
                     DCSTQEKKISEVSLGVFAFQRKAFQEILPLIDNSNAQGEYYLTDAVRVAREKGYTPEA
                     FAVEWDEQFLNVNTPADFSKAILILKERKIASLWDEGVKILDPSTTYVDWDVVVEEDV
                     WLYPGTVIEGKSVIEKGARIGPFSRISESKVGRNGSVEYSVVEGSTIGEEARVGPFAH
                     LRPGTKLERGVKVGNFVEVKNSHIGEETRALHLSYLGDAQIGKKVNIGAGTITCNYDG
                     EKKNPTFIEDEVFIGSNNSLVAPVSIGKGAYTAAGSTITQNVPPYSLGVGRAKQKNIE
                     DWTKRKTRRNKD"
     gene            11872..12840
                     /locus_tag="PKN31_02230"
     CDS             11872..12840
                     /locus_tag="PKN31_02230"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOA98780.1"
                     /translation="MLTSREFSRHLKVFCGTANPVLAQEVCWYLDIPLGKADVGRFPN
                     GEIRVQIEESVRGCDVFVIQPTSPPANDHLMELLIMIDALHRASAERVTAVIPFYGYC
                     KQDRKTKGREPISAKLVANLLVTAGTDRVLTMDLHAGQIQGFFDIPVDNLAAQSLLAR
                     YFLSRGWKEAVVVSPDVGGAARARSFANKLGADLAIIDKHRPTYSQVMVMNVVGEVEN
                     KRAIIVDDLIDTAGTLVEGAEALYRRGAKEIYACATHPVFSEPAFARIENSCLREVVV
                     TNTIEVSQEKLSQQGANKIKVLSVAPIFAEAIKRIHGELSVSELFI"
     gene            12861..13565
                     /locus_tag="PKN31_02235"
     CDS             12861..13565
                     /locus_tag="PKN31_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOA98781.1"
                     /translation="MSKGNNVVVNLERREEKGKEKSRKMRREGWIPAVLYSRDSRDGK
                     AELVKVREKELKRILQIPGITHHMLDLSLEGETRKGIIRDLQKNPIKEEIWHVDFYEV
                     QTGQRITLSLPVRIKGEARGVEAGGTLELVTQELEVECPSGAIPDEGIEVDVSELEIG
                     DVVRVGDLKVPQDVTIMEDPDEVIAVVIPPRVVEEEVEVEEEVEAGVEEETEEEAKEE
                     VGESQTTEKGKTEEEK"
     gene            13569..14138
                     /gene="pth"
                     /locus_tag="PKN31_02240"
     CDS             13569..14138
                     /gene="pth"
                     /locus_tag="PKN31_02240"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOA98782.1"
                     /translation="MYLIVGLGNPGEKYSHTRHNAGFRVVDRFSRQYNWKWQNIGHRL
                     SWTKGEVEGKQVYLLKPLTYMNQSGEGLLYFPRFKLFSEGILIVVHDEMDFPPGVVRI
                     KKGGGTAGHRGLNSLIETLKTDDFLRVRVGIGKPLSREEAIDYVLGVPEGEEKALLEK
                     GEERAVSALSSILEEGWERAMNKYNAQED"
     gene            14122..14727
                     /locus_tag="PKN31_02245"
     CDS             14122..14727
                     /locus_tag="PKN31_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98783.1"
                     /translation="MRKKIEVEKMEKKKLAETFSLVLQPSAIAVLLYLVVSFSFATGW
                     RAWFFFLIGLLFVGIFPILLVVFLTKKGKVSDPDLPNREERFFPYLMICGLYLLALVI
                     FLLTSAPLPLVAITCCYLGVTFVGAIVSLFWKISLHLAGVAGPVTAMAMIISPYWAFL
                     YLLLLPLGWARIYLAKHDLYQVVAGSVMSIVITFIIVSSIV"
     gene            14738..15481
                     /locus_tag="PKN31_02250"
     CDS             14738..15481
                     /locus_tag="PKN31_02250"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOA98784.1"
                     /translation="MDESLFKKAASFMLERKPWLVLTGAGISTESGIPDFRSPGSGLW
                     DKYDPMEILSTDVLFNRPHVFYQEGLPVLMKFEEAQPNRAHYILAEWEKEGLVEAVVT
                     QNIDSLHYKAGSQKVLEIHGHLRSGHCLKCRASFPIRVMKEKVDEGDIPPHCQCGGII
                     RPDVVLFGDMLPPCFEEAVELAHRLPLLVIGSSLQVSPANFLPSYSPYLIIVNLEPTP
                     FDQRAHFVLQGKASEVLWRLDEAMKNSGS"
     gene            complement(15470..16546)
                     /locus_tag="PKN31_02255"
     CDS             complement(15470..16546)
                     /locus_tag="PKN31_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOA98785.1"
                     /translation="MNNSEEGKIIKNFYYLILIVIALYLAYLLKDVLIPFALGGLLAY
                     ALSPVVQLLNSRGLSWKLSVIIVFVAILVFFLSLFIFLVPEVISQFRTLTFQWPQYSE
                     TLIEKIIEGLDKLDERYPDSNISQAVESFLTNISTNLQNYLTHVISGIPNLVSILANI
                     IFLGFIMTPVALYFFMADATKVRRSLVRLFPPSRRREYIALLREIDQVLGGFIRGRAL
                     IALFVGTCASVGLALMKIDFALAIGVIAGIVEVVPYLGPVLGAIPAIILAATKSPWLV
                     LGVILLFGGINFVEGIFISPQLLGKETGLHPLTVLFALAVGGKLYGALGLIIAIPVAG
                     ILKGLLSHHRKTNKLKTNKLSSTS"
     gene            16646..17356
                     /locus_tag="PKN31_02260"
     CDS             16646..17356
                     /locus_tag="PKN31_02260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA98786.1"
                     /translation="MLQSQKITVLIPTYNEAPRLPRVLEVVCKIKWVDEVVVIDSHSQ
                     DGTLDVARSFPVEVIPLDKDRGKGAALAEGIRLKKDSDIYVFLDADLIGLTEEHVLAL
                     VKPLIEDPSLAMTIGVFERGRRLGDLAQKFFPILNGMRGVRGEWVRSLPDFSWTRFGV
                     ETFLTRVALENKANVEFPALPGLAHYHKEEKYGFFLGFYHRIKMYKEIVQTLFLYKKR
                     IENWPKLVGEGEKHGVMV"
     gene            17435..18076
                     /locus_tag="PKN31_02265"
     CDS             17435..18076
                     /locus_tag="PKN31_02265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOA98787.1"
                     /translation="MKKIYDLTLPLTTDIPVFPGQPPLSLLPWSDLKIHGSATNAFFM
                     VEHTGTHLDVPAHFIEGGRTVEEIPLERFWGEALVLDVSSYLGRGELRLPDLERVLGE
                     KDIKEGDIVLFYSGVDKLLGKPEYFAQGVFLTEEVAKFLIDKKVKGVGTDSPSIDSQP
                     FAVHRLLLAREIIIYESLTNLRELWSKRIIFVGFPLKLKGCTGSPIRAVAICG"
BASE COUNT         5028 a         3300 c         4526 g         5303 t
ORIGIN      
        1 ctcgtcgaag gaaaagagaa gattcttcta tctcgcttcc tatcaaaaag actgatggag
       61 cggtagctgg tgtccttaat ttaaaccgga ctgactgccc cttcgaggaa gaggacattc
      121 cccagctgga agcactcacc attaatattg ctatcatcct ggaagaaaac agtcttcgcc
      181 ggaggcggga gcacattatt gttgccctat ctgaaatcgt tggccttttt tcctctttat
      241 tctgtactga tacctcaagt gatgtctttg agaaaatatt ttattcggtg aaaaccttga
      301 cgggtgcgag aaaggcagca gtattcaagc tttctaaaaa aagacctttt ctggtttttg
      361 agaaagattg gccaaaacag ctccggtgga aggattttgc tgaagttgat gaccaaatcc
      421 aggagtgtat tgagcaaaag gaagttataa ctattgaaca tagttcccga atttttttcc
      481 tctttatacc tttagtatca ggaaatcatc ctccctacct ttttacgggc attttgaaga
      541 agcctattac tattatcgat tatcttgtgc tttccattgt ggtgaatatg ggaaatgctg
      601 ctcttgagaa tatctccctc tatcaaaaga gtagaaggct ggctcaggag agagaaagaa
      661 atcgcctggc acgtgaactt catgatggat taacgcagat tatggttacc actcaaattt
      721 atcttcatct tctggaaaaa agtctggaaa atgccgattc caacgacctc ttgattttgc
      781 agaaagttca atctttgaat aaattgggtc tggaagaatc gcgctttatt ctttctgaac
      841 tgaagggcaa accaattact cctctacaat tccgggagaa agttgacgag actatcaatc
      901 tttttgctac tcctgacaaa gaggttgagt ttgactataa agtggatgtt aaagaaattc
      961 cttatcaggt ttaccaaacg gtgctgaaga ttttgcaaga aactttatct aacattcaaa
     1021 aacatgctca agctcagaaa gttaaaattt gtataatgaa tagcaaacaa cagatgattt
     1081 tttctataga agatgatgga gtgggctttg accctgaagc tttggaagaa aaagaatctg
     1141 aacattttgg tcttagaagc cttaaagaaa gggttcgggt tttgcgaggg aagtttgata
     1201 ttgatagtca accgggatgg ggaacaaaaa ttatggtaaa agttccctta ccgagagaaa
     1261 tagcggttta atttttagta tagagctatg aaaataagag ttgctcttat tgatgaccat
     1321 cccattttta ttgctggttt aaaacaactg ctggaaagtg atggccggta tgaagttaag
     1381 gcagtggctc aaagtggcga agaagcgcga aaatctattg attttaatga agttgatgtt
     1441 gctctggtag atgttaatat gccgggtgga agtgggattg agttaataag attcataaaa
     1501 cgacaaaaag gagactgccg ggtgataatg ttaacggttg aggaagatga agaaaccatc
     1561 aatcgggcga taaaagaagg agcccaagga tatatcctga aacaagaatc acctgaacac
     1621 ctgttaaaag ccattcaagc ctctatggat ggagaaatac tcttgagtaa tcatgtctat
     1681 actcgcatgg tagatagaat cagaaaaacc agtcccctgg ataaggaaca atcaatttta
     1741 tataataaat tgaccgagcg ggaaattgaa gttactcggt tgattgttca ggggaaaaat
     1801 aataagcaga ttgctcagga gctgtatatc agtgaaagta cggtaaaaaa tcatatcagc
     1861 aacattatgg ataagctatc gataaaaagt cgaacagagc tggtcactat agcagtgagg
     1921 gaggggattc aataatagta cttctcattt tccaatatag tacacttggc ttttaaaaat
     1981 aggaatttag tcctatatga agcaggttgt tttccattta tagtagtatt caggatttag
     2041 aactgcagag tctggaggat ggtaaattga caacggaggt caaaatcagc cagaaaaaag
     2101 tctttgttgt ggatgataac cgttttttcc tggaaagcct ggtgtttctt ctcaaccgtc
     2161 aaaaagatat aaaagtggtg ggtacttatc atcgtgcccg gggagttttg caaaaaatca
     2221 aagctgctca acccgatgtt gttattctgg atgtgcagct tcccgatggt gatgggatta
     2281 gtatcttgca ggagattaaa tctcagctgg gcattccggt aattatgctt actatttatg
     2341 aagaaggtag agagcgagct atgaaaaacg gggcttatgc ttatctggta aaaggagaag
     2401 aattggatat cctttaccag aagattcgcc agatcgggga gatttaaccg caagaatcat
     2461 tttaatctac ttcatttatg gggagagttg ttcttcgcgc tataaggcct cttctcaagg
     2521 agggttataa gaagaggcca cctactgccg ggggagacgc tttccccggc atagttttta
     2581 atcttacgtc gtagatactg agggatgctt tctccctcag taatttttgt ctttttattt
     2641 tgtttttagt gccgcaggct gcctttttag tcttacaccg taggtagttc tgtttccttt
     2701 ttatcttttt ctgtttttct tttaatccct taaagggggt gataggaggt ttccccctat
     2761 agtataatac tctggtgagt tttttgagaa ataaggtata atcctgtgtg gagatgaatt
     2821 tatatggcgg tagtagaaaa aaaagagaaa aacagcaagg aaggaaaaac agtcgaggtt
     2881 gctttccgct ctatcaccaa acgttttcct cctgacattt tagctaacga ccgggtatct
     2941 tttgaagtag aaaaaggcac tgttcacgct ttactgggag aaaatggagc aggcaagaca
     3001 acgctcatga atattctcta tggacttttt ccccccgatg aaggagaaat aatcattcgg
     3061 ggtatgccca ctaagatttc ttcccctcgg gaggccatta atctggggat tggcatggtt
     3121 caccagcatt ttaagctcat ctctactcac acggttagtg aaaatatcat tctggggtta
     3181 gcatctactc cttttttctt tcccgcagaa aaagcggaat ccgaaatcag gtttctctca
     3241 gaaagatatg gtttgaaaat agatcctcga gctcgtatct ggcaactttc agcgggcgag
     3301 aagcaaaaag ttgagattct taaagctctt tatcgccggg ctgaaattct tgttctggat
     3361 gaacctacct cggttctgac tcctcaagaa gcagaagagc tatttgccac tctccggttg
     3421 atgaaagaag agggtaaaac ctgcattttt atcactcaca aactcaatga ggttaaagag
     3481 attgccgatg ctgtgacagt gatgcggaga gggaaggtga tttctactat tcttccctcc
     3541 tcggtttctc tggaagagtt agcccgaatg actgtgggaa aagaagtgat ttctgtggtt
     3601 ccccgtaaaa ccgtatcagg taaaattatt cttcagcttc agaaagtttc cgctatggga
     3661 gatagagaaa ttcctgctct ttcaaatctc tctcttaccg taagagaggg agaaattgta
     3721 ggggtggcag gtgtggcggg aaatggacag aaagaattgg cggaagtaat agtgggttta
     3781 cgtcaggcta cagaagggca tgttttctta caagataaag aaataactaa tctcccgcct
     3841 cgagatatcg cttctttagg agtagcttat attcctgaag agcggcatat ggggttggtg
     3901 ggggagatga gtgtagcgga aaacctcctg ctgcgaggtt attctcggcc tccgttttct
     3961 cagggtttct gggtggactg ggaaaaggca gaagcttaca gtgaaaaact tatttccaat
     4021 tatcacatta atccacctat tgcggatatt ccggtgcggc ttctttcagg aggaaaccgc
     4081 cagagagtaa tcattgcgcg ggagctggag cggaaaccaa aattggtgat agcttccaat
     4141 cccactgctg gtttggatgt ttccggtgta gaggctattc atgagttact ggtaaaagct
     4201 caagaagaag gtgcaggaat tcttcttatt tctggagacc tggatgagct tttgaaactt
     4261 tccaatagaa tagtgattat gtttaaggga gaaattgtag gagagaaaga gaaagagcac
     4321 tggacagaag aagatcggcg agaaattggg ttggggatgg gaggcgtaag gatataagtg
     4381 ggtagaagaa aacttacgtc gttccgcgta gagttatcgc tacaaccaac tctttactct
     4441 gtcatctttt tctctctttt ggctattgtt cttgcttttg catgtggcag cattattttt
     4501 ctgttctacc atatttctcc ctggatagct tatcaaactt ttttccgggg agcttttggg
     4561 agctggtacg ctttttccga gactctaaag cgaaccattc ctctactttt ggtaggtgag
     4621 gggttagctt tagcttttcg ggctcaagtg ctcaatattg gagcggaagg gcaaattctt
     4681 ctgggagctg ttgccgctac ctgggtggct cttttttccg ggcttccctc ttttctttta
     4741 cttcccacga tgtttctgct gggattctta gctggagcag gatgggcttt gattcctgct
     4801 ttctttaaaa ctcgttttgc agtgaacgag gttctttcta cccttatgct taactatgtg
     4861 gcttctaatc tggttctcta tctaattgga ggtccctgga aaggtaaagg agtgcgaggt
     4921 tttacctaca ctgatacttt tcctgaggcg gcctggctta aaacgatggg gataaccaga
     4981 gttccctatt ttactctttt aacaggcatt gttctggctg tggggggcta tattttcctt
     5041 aaaaaaagcg ctttaggttt tgagttccgt attgtgggta acagttctga aacagcaaag
     5101 cttctgggaa taagtgagga aaaaattgtt cttttcgcga tgttcctctc aggggggtta
     5161 gccgggtttg caggagtagg agaggtcgct ggtatacatc atcttttgag gcatcccgac
     5221 catatttcct taggttacgg atacacggcg ataattgtgg cctggttagc tcgagccaat
     5281 cctctggcta caatttttac ctcttttttg tttggtgctc tcctctccgg tggcgatgct
     5341 ctccgggtgt cgttgggtat tccttttcag gtggtatcgg tattcaatgg acttattctc
     5401 ttttttctca tttctactga acgtttgagc cgatatcgta tcatatgggg ggaggagaaa
     5461 gcggtggaga gaagtggctc ttgaacttgt tttaggaacc ttaaatcggg ctctggctta
     5521 tggcactccc cttcttttag ccacgctggg agagatttat gtcgagcggg ccggggttct
     5581 aaatctgggg atagagggaa tgatgatgct gggggcattt tcctcttttt gtgtggctta
     5641 tttgacttct aacccctggt tgggaattct gacgggagcc atagcgggag gattgttctc
     5701 tctgattcat gctttttcta gtgttactct gcgtgccaac caaactgtat cgggactggc
     5761 tctcactatg ttgggaacgg ggattgctga aactttggga agaagttggg agggaaaggt
     5821 gctcacctca cctatcagac ccttctcgga agactttttg tctctgttcc ctctctggat
     5881 gagaaccctc tttagagata gcaatctcct tgtggtgggg agtatcattc tggctctggt
     5941 gctctggttt tttcttttta aaactaaacc gggacttttt ttgcgttctt gtggggaaaa
     6001 ccctgcagct tgtgatgctc tgggggtcaa tgtatggaaa gtacgctata tagcagtttt
     6061 ttggggagga attatggctg ggttaggagg ggcgtatatt gctgttgcct atcgtcccac
     6121 ctggtcttca ggtatgactc aggggatggg atggttagca gtggcattaa ctattttttc
     6181 tttctggaat cctctctatg ctcctcttgg tgcttacctt ttcggagcat tgtttcactt
     6241 agcttttcgc ttacaggttt tcgttgctcc agaaatttta aattccctac cttatctttt
     6301 cccggtggtg gctctggtgt tcttttctcg tctcgctttt cggaaaaaga ttggtgttcc
     6361 gggaagttta ggaaaacctt atgagcgggg agaatcagag taaatatagg aaactaagaa
     6421 aggagtgaaa aaattggaag cgtctgtgga tcagttaacg gtcaaaacta ttagaatgct
     6481 ggctctggac acgatccaga aagcaaaatc gggacatcct ggattacctt taggagctgc
     6541 tccgatgggt tacgttcttt ttcgacgggt tatgaaacat aatcctaaaa atccccggtg
     6601 gtttgatagg gatcgtttta tcctctcggc agggcatggt tcggccttgc tttatactct
     6661 tctttatctg agtggatatg agaagatgag tctcgatgaa ctaaagagtt tccgccagtg
     6721 gggaagccat acccctggac atccggaacg ctgcatagat tgtggtatag aagctacaac
     6781 tggccctttg ggacagggct ttgcgatggg cgtgggagcg gcaattgctg aagctcacct
     6841 tgctgcccga ttcaaccgtc ctggctttcc cattattaac cactatacct atgctctggt
     6901 gagtgatggg gatttaatgg aaggagtggc gcaggaggcg gcttctttag ccgggcattt
     6961 gaaactgggg aaactgattt atctctttga taataaccgg gtgagtcttt caggagagac
     7021 gtcaatttgt tttactgaaa acatcagaat gcactttgaa gcttatggat ggcaggtgct
     7081 tgaagtaaaa gatggaaaca atttagaaga gcttgttcgg gcaattcagg aagcacaaaa
     7141 agatgaaagc cgcccctctc ttataatggt ttctactcat atcgggtatg ggagtcctaa
     7201 acaggatagc tttgaagtcc atggctcacc tctaaaacca gaagaggtag tagagacgag
     7261 aaagtttttc ggctggcctc tggatcagga tttctatgtt cccgaagagg ctttaaatca
     7321 catgcgagaa attataaggg aaggtgaaga aaaggagaaa gaatggaata acctcttttc
     7381 ttcctatgct cgagagtttc ccgaattggc acaggagttg accatgctta taaagggaga
     7441 gctccctccc cggtgggacg aaggccttcc tgtttttgat cctgcttcag gagacattgc
     7501 cactcgctcg gcaggaggca aagttctcaa cgccctggca gataaaattc cttctctcat
     7561 cggtggctcg gcggatttag acccttccac aaacacagtc ttgaaaggga aagggagctt
     7621 tcaggcgccc ggttcgtgcc cccctggaac tcaaggtctt gtggaaggac ctctgggtta
     7681 tgagggaaga aatattgctt ttggcgtgcg agagcacgct atgggggcta ttttaaatgg
     7741 tattgccatt catggggggc ttattccttt tggggcgaca ttttttgtat tttctgatta
     7801 tatgaggcca gccactcgag tggcagctct ctcccattgt aaagtcattt atgtgtggac
     7861 tcatgattcg gtagctctgg gggaagatgg gccaactcat caaccggtag aacagcttat
     7921 gagctttcgg gcaatgccta atttagtttg cattcgaccg gctgatgcta atgaaactcg
     7981 tgaagcgtgg aaaatagcca tagaaaggaa agaagaaccg gtagcgctca ttttaacccg
     8041 gcaaaacgta cctgtaatcg atcgggataa atatggaaaa gcggaaggct tgagaaaagg
     8101 agcctacatc atcgccgatt gtgcaccgca tttacctcag ctcatcatta ttgctacagg
     8161 ttcagaagtg agtctggctc tggaggctta tgagaaacta tcttctgagg gagtccgggt
     8221 gcgggtagta agtatgcctt cctgtgaatt atttgaagaa caacctgagg catatcgccg
     8281 ggaagttctt cctccggaag tgaaaaaacg aattgccatt gaagctggag caacactggg
     8341 gtggtataaa tatgtagggg aagagggtaa ggtcattggc attgatcgct ttggcgcctc
     8401 tgctcctggt aaagtggtgc tggaaaaatt aggtatcagt ctggaaaatc ttttagctca
     8461 agctcggtct cttcttgaat aaagagagaa gtgcaccttg aaaattgaat aatgtataca
     8521 ataggggcat cctggaaaag aaataaactt agaagcggaa gattaaaaat cggattaaaa
     8581 atataaaaga acaaaaacta ttggggagag aagacgtctc cccggatacc ccctataaac
     8641 tctaaaattg ccatgcatgc tggaatcttt tttctccggt gttattatct cggtttttag
     8701 tctttcctac ctttttcttg cgctcaggca ccagatgaga tttgcaatga cagctgaagg
     8761 taagggatag atggtaaatc gggattgcca tggtaagcga aacaaatgat cctcttgacg
     8821 gtggcacgag atgtgagata ttttgtagca aataaaggga gttgagttgt gcagcaatcg
     8881 atagaactat gcttatttag tttgatgtca caagattttg gaacttccga aaatttcatt
     8941 atcatgctta aggaggcgtt tcaatgaata aaaaggcagt tttagcagtg gttcttcttt
     9001 tgtctttgct tattacagga gtagcttttt cagaagaggc taagttgaag gcaggattta
     9061 tctatgtagg gccaataggg gatttaggct ggagtcatgc tcatgagcaa gctcgtcaag
     9121 tgttagaaga ggtctatccc tggctggata ccatatatgt agaagcagtt ccagaaggag
     9181 aagtagaagg aatcatcgat cgattaatta atcaagaaaa agtggatata gtggtgacca
     9241 caagttttgg ctttatggat ggaactttga atgcagccaa aagatacccg gataaaatct
     9301 tctttcattg ttccgggttt aaacgagctc ctaatttggc cacctatatg gctgatttcc
     9361 atcaagttta ttatttgaac ggaattatgg ctggagcact aactaaatcg ggaaagattg
     9421 gttacgtggg gactttccct actccggaat taaaaaggca cattaatgct tttactctgg
     9481 gagtgcgccg ggtaaatccc caggcagagg ttaatgtgcg gtggttgcaa agtgcctggt
     9541 atgaccctca aggggcaaaa gaggcagctg aagctctcat tgctgaggga atagatgttc
     9601 tggcttttac tgaagattcg gcaagcgtgg ctcaggttgc agaagaaaaa ggacttccca
     9661 gttttggtca ttattctccc atgttgtcct atgcgcccaa tcatctagtt tccgggcaat
     9721 tggtacactg ggaggcaatt tatctcgact tttttgctaa ggtgtatgct ggactctata
     9781 acaatgaaaa tttggaaaac gtcgattact ggtggctttt aagcaacgga gcagtggagt
     9841 taggagctga ctttggcgtg cctgttaacc ctgcatttga agaagctttg aaaagctata
     9901 tggtaaatga cccggtatat ggagaaatga gcgcctatga tttagtcttt gagttagaaa
     9961 aagctatggc cgaccctgaa tatgctttca gtccttttac tggacctata aaagaccgta
    10021 aggggaatct acttgttccc tcttcggttc gtcttgctct tgaagatttg atcaccatgg
    10081 agtgggctgc agaaggagtg gctggcccct ggcccggaga accagagtaa tctgagaggg
    10141 agagattctc tccctctttt tttatgatta tgaatctatt cctagacggt gagaatttga
    10201 ttgacagggt ttttttactc ttatataata aaggagctat tggggcgtgg ccaagcggta
    10261 aggcaaggga ctttggatcc cttactcgga ggttcgagtc ctcccgcccc agccatcttc
    10321 ttttagttct ctcatcgaat agttaagtct tacagctttc ctcaattgat agctaaagat
    10381 gaagaatctg agttgattaa ggatagaaat ttgataaaat agttaatagt ttccataaat
    10441 cgggcgtgct ttggtcacgt tttgtctggt gtggtgaaaa atatggatct ttctcaatgg
    10501 ggtttaataa ttttagccgc gggtcagggt aaaaggatgc gctcttctct tccgaaggtg
    10561 cttctccctg cctggggtag gcctattatg caatatttac tggatgagtt gaaatgggaa
    10621 gagttcggtc aaagatttct ggtggtttct ccctctatgg aagagttgat aaaaagtagt
    10681 tttggtcagg atataaccat agtggttcaa aaagagccta aaggtactgc tcatgctgcg
    10741 agtactgttt tccccctgat gcaagaaggc attgattggt ttttaatact ttatgctgat
    10801 atgccccttc ttcagggaga aactattttt tcttttctct ctcgagtgga ggaaaatcgc
    10861 tatccctttt cttttctaac ttctttttct cctgaccctg ctggctttgg acgggtagtg
    10921 cgggatgaaa aaggagaacc tctggggatt gtagaggaaa aagattgttc cacccaggag
    10981 aaaaaaatat ccgaggtgag cttgggggtt tttgcttttc agagaaaagc ctttcaagaa
    11041 attcttccct tgattgacaa ttctaacgct cagggggaat attacctaac tgatgcagtt
    11101 cgagtggctc gagaaaaagg ctacacgcca gaggcttttg ccgtggagtg ggatgaacaa
    11161 tttttgaatg ttaatacccc tgctgatttt agcaaagcta ttttaatatt aaaagaaagg
    11221 aaaattgctt ccctgtggga tgagggggta aaaatattag acccttctac tacctacgtt
    11281 gattgggatg tggtggtaga ggaggatgtg tggttatatc ccgggacagt catagaagga
    11341 aaaagcgtaa tcgaaaaagg agctcggatt ggtccttttt ctcgaatatc ggaaagcaag
    11401 gtgggaagga atggctctgt agaatatagt gtggtggagg ggtctaccat aggagaagaa
    11461 gcgagagttg gcccttttgc tcatttgcgt ccgggcacca aactggagcg aggagtaaag
    11521 gtgggcaatt tcgtagaggt gaaaaattct catattgggg aagaaaccag agctttgcat
    11581 ttaagttatt taggagatgc acagataggt aaaaaagtaa acattggagc aggaaccata
    11641 acttgtaact atgatggaga gaagaaaaat cccaccttta tagaagacga agttttcata
    11701 ggaagcaata attccttagt ggcaccggtg agtatcggaa aaggtgctta tacagcagcc
    11761 ggctctacca taactcaaaa tgtccctccc tattctctgg gggtggggag agccaagcag
    11821 aagaacatcg aggattggac gaaaagaaaa acacggagga ataaagacta aatgttaacc
    11881 agtcgggaat tttcccgcca tctaaaagtt ttttgcggta cggctaaccc tgttctggct
    11941 caggaagtgt gctggtatct cgatatcccc ttaggaaaag cagatgtagg gcgctttccc
    12001 aatggagaaa taagggtgca aattgaggaa agtgtgcgtg gttgtgatgt ttttgtgata
    12061 caacccacct cccctccggc caatgaccat ttgatggagc ttttaataat gattgatgcc
    12121 ttacatcgtg cgtcagcgga gagagttacg gcggtcattc ctttctatgg ttattgtaag
    12181 caagaccgaa agactaaagg tcgggagcca atttctgcaa aactggtggc taacctttta
    12241 gttaccgccg gtacagaccg ggttttgacc atggatctgc atgctggaca aattcagggt
    12301 ttctttgata taccggtaga taatttggct gctcagtctc ttctggctcg ctatttcctc
    12361 tctcgaggat ggaaagaagc ggtagtagtt agccctgatg tgggaggagc agctcgagct
    12421 cggtcttttg ccaataaact gggagcagat ttagcaatta ttgataaaca ccgacctact
    12481 tactctcaag tgatggtgat gaatgtagtg ggggaagtgg aaaataaaag agcgatcatt
    12541 gtcgatgacc tcattgatac tgcgggaact ctggtggagg gggctgaagc gttatacaga
    12601 cggggagcta aagaaatcta tgcctgtgct acccatcccg tttttagtga gccagctttt
    12661 gctcgaattg agaattcttg tctccgggaa gtagtggtta ccaataccat tgaagtaagc
    12721 caggaaaaac tttctcagca aggagcaaat aagataaagg tactttctgt ggctcctatt
    12781 tttgctgaag caattaagag gattcatggt gaattgtcag ttagtgaact ttttatataa
    12841 gaagagcgag gtaagataat atgtcaaaag gcaacaatgt tgttgttaat ctggaaagaa
    12901 gagaggaaaa aggcaaagag aaaagccgaa agatgagaag agaaggctgg ataccagcag
    12961 tcttatattc tcgagatagc agagatggaa aggcagagct ggtgaaggtg agagaaaaag
    13021 aactgaagcg catcttacaa ataccgggta ttactcacca tatgctggat ttgtctttag
    13081 agggagaaac gagaaagggg attatccgcg atttgcaaaa gaatcccata aaagaagaga
    13141 tctggcatgt ggatttttat gaagtgcaaa ctggtcaaag aataactctt tcactacccg
    13201 tgagaataaa aggagaagct cgaggggtgg aggccggtgg gactctggag ttagttactc
    13261 aagagttgga ggtggagtgt cccagcggtg ctatacctga tgaaggtatt gaggtagatg
    13321 ttagtgagtt ggaaattgga gatgtcgtcc gggtgggaga tttaaaagtt cctcaggatg
    13381 tcacgataat ggaagaccca gatgaagtga tagcagtagt tattcctcct agggtagtag
    13441 aggaagaggt agaggttgag gaagaagtag aggcaggagt agaagaggag acggaggaag
    13501 aagctaagga agaggtagga gaatctcaga caacagagaa agggaaaact gaggaagaaa
    13561 aatagttggt gtatttaata gtaggtctgg gcaatccagg agaaaaatac tcgcataccc
    13621 ggcataatgc gggttttcga gtagtagatc gtttttctcg ccaatacaac tggaaatggc
    13681 aaaatatcgg tcatcgtctt agctggacaa aaggagaagt tgagggaaag caagtttatc
    13741 tattgaagcc ccttacctat atgaatcaaa gcggggaggg gcttctctat tttccccgat
    13801 ttaagctttt ttctgaggga atcttaattg tggtccacga tgagatggac tttcctccgg
    13861 gggtggtgcg aatcaaaaaa ggaggaggca ctgccggaca ccgaggattg aattctctaa
    13921 tagaaacctt aaaaacagac gattttttac gcgttcgagt gggaataggt aaaccacttt
    13981 ctcgagagga agccatagat tacgttctgg gagtgccaga aggggaagag aaagctctct
    14041 tagagaaagg agaggaaaga gcagtttctg ctctttcctc tatcttagag gaaggatggg
    14101 agagagctat gaataaatac aatgcgcaag aagattgagg tggaaaaaat ggaaaaaaag
    14161 aaattagctg aaactttctc cctggtgcta cagcccagcg ctatagcagt gcttttatat
    14221 cttgtggtga gctttagttt tgcaacaggc tggagagcct ggtttttctt tttaattggc
    14281 ctgctttttg tgggcatatt cccgatacta ctggttgtct tcttaactaa aaagggaaaa
    14341 gtttctgatc ctgatttgcc caacagagaa gagcgttttt ttccttattt aatgatttgt
    14401 ggtctttacc ttttagctct ggttattttc cttcttactt ctgcaccttt acctcttgta
    14461 gctattacct gttgttatct gggagtaacc tttgtgggag caattgtttc tcttttctgg
    14521 aagattagtc ttcatctcgc tggtgttgcc ggtcctgtta ctgcgatggc catgattatt
    14581 agcccttact gggcgtttct ttatcttttg cttttgcctc tgggctgggc gagaatttat
    14641 ttagcaaaac atgatttgta tcaggttgtg gctggaagtg tgatgtctat agttattact
    14701 tttattattg tatcaagcat agtttaagga gagaaagatg gacgaaagtt tatttaaaaa
    14761 agcggcatct tttatgctgg aacgtaaacc ctggttggtg ctcactggag caggaatatc
    14821 aacggaaagt ggcatccccg attttagaag cccaggtagt ggtttgtggg ataaatatga
    14881 ccccatggag attttatcta ccgatgttct ttttaatcgc cctcatgttt tttatcagga
    14941 aggccttccg gttcttatga agtttgaaga ggctcagcct aatagagctc actacattct
    15001 ggctgagtgg gaaaaggaag gtttagtaga ggcggtagtc acccaaaata tagacagctt
    15061 gcattataaa gctggttctc aaaaagtgct ggagattcat ggccatcttc gctctggcca
    15121 ttgcctcaag tgtcgggctt cttttcccat tagagttatg aaagaaaaag tagatgaagg
    15181 agacatacca ccccattgcc agtgtggcgg cattatccgt cctgatgttg tgctgtttgg
    15241 cgatatgtta cctccttgct tcgaagaggc cgtggagctg gctcatcgtc ttcctctttt
    15301 ggttattggt tctagtttgc aagtttctcc ggccaatttt ctcccttctt atagtcctta
    15361 ccttattata gttaatctgg agcctactcc ttttgaccag cgagctcatt ttgttttaca
    15421 agggaaagcc agtgaggttc tctggagact cgatgaggct atgaagaatt caggaagttg
    15481 aggagagttt attagttttc agcttattag ttttccggtg gtgggaaagc agtcctttta
    15541 agataccggc cactggtata gcaatgatta accctaacgc cccataaagc tttcccccca
    15601 ctgcaagagc gaaaagaaca gttagaggat gaagaccagt ttctttgccc agaagctggg
    15661 gagatataaa tattccttct acaaaattaa tgcctccaaa aagaagtatt actcctaaaa
    15721 ccaaccatgg tgatttagta gctgctaaaa taatagccgg tatagcacca agcacgggac
    15781 cgaggtaggg gactacttct actattccag caataacccc tatagccaga gcaaaatcta
    15841 tcttcatcag ggccaagcca acactagcgc atgttcctac aaaaagggca attaaagctc
    15901 tccctcttat aaaccctcct aaaacctggt caatttcccg gagtaaggct atgtattccc
    15961 ttcgtcgaga aggaggaaac aatcttacta aagagcggcg gactttagta gcatcagcca
    16021 taaaaaagta aagagccaca ggggtcataa taaagcctaa aaagataata ttagccagga
    16081 tagatactaa gttgggaata ccgctaatta cgtgagttaa gtagttttgg agattggtgg
    16141 aaatattagt taagaaactt tctacagcct gagaaatgtt tgaatctgga tagcgttcat
    16201 caagtttatc taatccttca attattttct caatcagggt ttctgagtac tgaggccact
    16261 gaaaggtcag ggttctaaat tgagaaatta cttcgggaac cagaaagata aaaagagata
    16321 agaaaaatac cagtattgcc acaaacacta taatcaccga gagtttccag gagagccccc
    16381 gagaattcag gagttgaaca acaggagaaa gagcataagc taaaagccca cctaaggcaa
    16441 agggtattaa aacatctttt agtaaatagg cgagataaag agcgatgacg atgagaatga
    16501 gatagtagaa attttttatt atttttcctt cttctgagtt attcattttc cctttccctg
    16561 ctttatatga tagtattatt gtaaacgtac aaggggagat ttccaatagg ggaaataata
    16621 aaattgaaga acgggtgaag aagtaatgct acaatctcaa aaaatcaccg ttttaattcc
    16681 cacttataac gaagctccca gattacccag ggtgctggag gtagtctgca agatcaagtg
    16741 ggtagatgag gtagtggtaa tcgatagtca ctcccaggat gggactctgg atgtagctcg
    16801 tagttttccg gtagaggtta ttcctctgga taaagacaga ggaaagggag cagctcttgc
    16861 agaaggaatt cggctgaaga aagacagcga tatttatgtt tttctggatg ctgatttgat
    16921 tggtcttact gaagagcatg tcctggcgct ggttaagcct ttgatagaag acccctcttt
    16981 agctatgacc attggtgttt ttgagagagg tcgaagattg ggtgaccttg ctcaaaaatt
    17041 ttttcccatt ctcaacggga tgcgaggagt gcgaggagag tgggtgagaa gcttacctga
    17101 tttttcctgg actcgttttg gagtggaaac ttttctgacc agagttgccc tagaaaataa
    17161 ggcaaatgta gagttcccgg cacttcctgg tttagcccac tatcataaag aggagaaata
    17221 tggctttttt ctgggatttt accatcggat taagatgtat aaagaaatag tgcaaactct
    17281 ttttctttat aaaaaacgaa tagaaaattg gcctaaactg gtaggagagg gagaaaaaca
    17341 cggtgtcatg gtatagcgta atataaaatg aattttatca gttgtttttt aattatggtc
    17401 gtttcatgag ttggtttttc ctccatcaac cgaaatgaag aaaatttatg atttgaccct
    17461 tcctcttact accgatatcc cggtttttcc cggccaacct cctctttctc ttttaccatg
    17521 gagtgacttg aaaattcatg gtagtgctac caatgcgttt tttatggtag aacataccgg
    17581 cactcatctc gatgttcctg ctcacttcat tgagggagga agaacggtgg aagagatacc
    17641 cctggaacgt ttctggggag aagctctggt tttagatgtc tcctcttacc ttggtagagg
    17701 agagctaaga cttcctgacc tggaaagggt tttaggagaa aaagatataa aagagggaga
    17761 cattgtactt ttctatagcg gtgtagataa gctattgggt aagccagaat actttgctca
    17821 aggggttttc ctgactgagg aagtagctaa atttttgata gataaaaaag taaagggggt
    17881 aggaactgat agtccgagta ttgattccca gccctttgcg gttcatcgcc ttcttttagc
    17941 tcgagaaatc atcatttacg aatctttaac caacctcagg gagctctgga gtaaaagaat
    18001 cattttcgtc gggtttcctc tgaaactaaa agggtgcact ggctcaccca ttagagccgt
    18061 agctatttgt ggttaactgt aaatattatg ccgaggggag aagatttttt gttttcccct
    18121 cggcataatt cggtctctaa gcagtttttt tgttttt
//
LOCUS       DAOPSS010000019        17832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2660_length_17832_cov_9.047477, whole genome shotgun sequence.
ACCESSION   DAOPSS010000019 DAOPSS010000000
VERSION     DAOPSS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 17832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17832
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2660_length_17832_cov_9.047477"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PKN31_02270"
     CDS             <1..948
                     /locus_tag="PKN31_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOA98788.1"
                     /translation="RLGFDRVFDTNFAADLTIIEEGTEFINRLQKGGKLPLITSCSPG
                     WIKFMEHFYPELMENVSTCKSPQQMFGAVAKTYYAQKLGIDPQDMVVVSIMPCTAKKF
                     ECQREEMKSSGCQDVDYSLTTREAARMLKEKGIDLREMPEEEFDDPLGISTGAAAVFG
                     ATGGVMEAALRSVYEIVSGKTLESLDFYDVRGMEGVKEATVNIDGLEVGVAVAHSLGN
                     ARKILDQIKEGNSKYHFIEIMACPGGCIGGGGQPIPTDLDIRMKRIEAIYQVDKNLSL
                     RKSHENPAIKQLYEEFLGEPNGEKAHQLLHTHYLPREKM"
     gene            1094..1540
                     /locus_tag="PKN31_02275"
     CDS             1094..1540
                     /locus_tag="PKN31_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98789.1"
                     /translation="MIFAVDYNSEEEVILSIDPGKEKVGVAVLSSQGEVWFRKILTEE
                     EVEEEIQILKKQYGYSQIVVGDSTGKEKILEILRKMGLAFALVDERNSSEEARKLYFR
                     AHPPRGWRKIVPLSFLYPDAPFDDWQAVVIGRRYLDQKKSEKNGRK"
     gene            1527..2066
                     /gene="amrA"
                     /locus_tag="PKN31_02280"
     CDS             1527..2066
                     /gene="amrA"
                     /locus_tag="PKN31_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOA98790.1"
                     /translation="MEENSELRKVIPSLARRAIEYYLKEGKYLPLPPGLPSYLISEKR
                     GVFVSLKKKGRLRGCIGTYIPRYENIAQEIIHNAVSAATQDPRFPPLQEEELPFVSVS
                     VDILSYPEEVNSLEELDPKKYGVIVEKGWKRGLLLPDIEGVDTVDEQIRIALAKAGIS
                     PPEKFTIYRFTVDRYEEED"
     gene            2063..3064
                     /gene="amrS"
                     /locus_tag="PKN31_02285"
     CDS             2063..3064
                     /gene="amrS"
                     /locus_tag="PKN31_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOA98791.1"
                     /translation="MKVARFFTDLENKRVQCFLCPHRCIIAEGRNGTCRVRKNDGGVL
                     YAQTYGKISSFALDPVEKKPLYHFYPGRNIFSVGSVGCNLHCSFCQNWSISQTNPDNF
                     SLREISPEDLARRAQRDGSIGLSYTYNEPFIWWEFVYDTAQLIRKEGLKNVLVTNGYV
                     EEEPLRELLPWIDAMNVDLKSMDDDFYRRYCGGTLQPVLKTIEISVREGTHVEITNLI
                     IPGLNDRWDLIEKLVSWIYDLNPSIPLHFSRYFPTYKLDNPSTPVSFLEEVYHFARER
                     LPFVYLGNIGGGKGNITFCPTCRREVVIRRGYQVDTSGLLGNRCRFCGSSLPFYHET"
     gene            3084..4013
                     /locus_tag="PKN31_02290"
     CDS             3084..4013
                     /locus_tag="PKN31_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOA98792.1"
                     /translation="MFFIFLFSGFSFRDKEEKKTFFGFDTWIEVELPQDSALLFPRIS
                     SLVTHLDQLWNRFSPRSMVFKINHSSFPLSVDEDTFRILEEAQKGGEETQGFFNILIA
                     PLLDLWGFPGQPSIPGKEEIEETVDKIGKSKLHLEKESKRVFIEGEGQIDLGGIAKGY
                     LIDEIVSLLQKERVSQALINAGGEIFALGKEIKAGVRHPREEKLLGYIIIKDEAVSTS
                     GDYYRFFEEEGVRYHHILNPFTGYPGRDFWSVTVVSKKATLADILSTVIMAGGREALV
                     VVEKNFPEIKILAVEEGGNVYLSPSMEKVFFTE"
     gene            4016..4831
                     /gene="mreC"
                     /locus_tag="PKN31_02295"
     CDS             4016..4831
                     /gene="mreC"
                     /locus_tag="PKN31_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOA98793.1"
                     /translation="MNGNRNQKLLILIVLVVISSQILIWAVRAEDNKIENLHSFFLPL
                     YRGSEWIKDIFWGWGKFFQDQKSLREENQKLKEELEKLRADNILLQERIEELKIKIEA
                     GAMEEKLPFEVIPAQVIGRDPYDWWGKAIIDQGKEEGVGEGLVVVTYQGMVGKIEEAY
                     PHHSVVRLLLSPSLSVGVLVQRTRDLGVVTGNGEGLCIMKYIYRTSDLREGDIVITSG
                     WGENTPRGVVVGRVSQIGEDDGSLFKDVIVEPSCDFSLLDKVFVIKALSEKLE"
     gene            4897..5316
                     /locus_tag="PKN31_02300"
     CDS             4897..5316
                     /locus_tag="PKN31_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98794.1"
                     /translation="MYLWFPLSHLHIFSFLMVSLFWEGKESTLDFLLILLLGITSDLL
                     LFSPWGAYLFILSSLALFTYLWVKFFSTSFVSLSVIFFCLPLLELGLEWVVAALGETT
                     LPFLPLLGAKLLSIPLNIFLFYWWQKRETRRGLEYVE"
     gene            5306..7060
                     /gene="mrdA"
                     /locus_tag="PKN31_02305"
     CDS             5306..7060
                     /gene="mrdA"
                     /locus_tag="PKN31_02305"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOA98795.1"
                     /translation="MWNDEIQERVNQRITYLSRLFLIILILLSFRFWYLSVLQGDWYK
                     QKSEQNRVRIFSLPPIRGSIYDRNNQVLAEDVPRFRLIFEKGGVEVEEIEKRVERILG
                     RELSPPFPERAVGEIILVDQLSMEEVVKIEEAQNELPGVIVESYPVRHYPCGEPFAHA
                     VGYVGKINAEEFKMLRSEGYEMEDRIGKTGVELEYESLLRGEKGYRRIEVNALGKVVG
                     VLDYRPPYFKNSVVLTLDRELQEYSYDLLSNKNGVIIVGDPYTGELFALVSKPSFDPQ
                     LLSWGMSAEEWNALSQNKDRPLTNRPLQALYPPGSLFKLVIATGALEEGLTGRNRKIF
                     CPGFLDYNEWRYPCWRRGGHGSVGIEEAIAQSCNVTFYTLGLELGPDKIINWANRLGV
                     AQPSGIDLPGEKNGFLPTPEWKKRQLKEMWYPGDTINLSIGQGYLLVTPLEMYRVVSS
                     IATRGEVYKPHVLKKVLNSKREVVREVAPVLQDRIELRNETWESLIRGMEKVVNSGTG
                     RVCRGLPVKLAAKTGTAQNPQGEDHSWFSGFFPSSHPQVAFLVLIEHGGDGSGEAAQT
                     ARKLIEWWEENRGGKSGK"
     gene            7050..8150
                     /locus_tag="PKN31_02310"
     CDS             7050..8150
                     /locus_tag="PKN31_02310"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOA98796.1"
                     /translation="MENKVEDKAMVLVLIALSLIGVLAVYSVDPLRGEGWGESLFSRQ
                     IIWNLIGWVAFLFFSQIDYYQLVKRGWLWYFLMVLSLGAVLIFGEGDETGSRRWLFSQ
                     SVQPSEGAKVALALFLAHYFSEQRESFDFWPLLIKSGILSFIPLVLIFEEPDLGSSLV
                     LLVIWLGALFVCGFSAKKWAVIGGIGGGLLPLSWVFLEDYQKDRLLSFIDPGRDPLGS
                     GYHILQSQIAIGAGGFTGQGWLSGTQSQLYFLPARHTDFIFASWCEQLGFLGGMVIIF
                     LLGLLSYLFLVVALRSPNLEGKILASLLGISLIFQWTVNIGINLGVMPVTGIALPFIS
                     YGGSSLVVNLASAGLVYNISKSGGKESWKLWI"
     gene            8141..9925
                     /locus_tag="PKN31_02315"
     CDS             8141..9925
                     /locus_tag="PKN31_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOA98797.1"
                     /translation="MDLEKTLSRQEMLSISKPARYIGREWNISLKKEEDVEISVALAF
                     PDLYEIGMSHLGLKILYYILNSLPGVRAERAFAPWPDMGKMMKEKNIPLYSLENKIPL
                     RNFDAVGISLQHELNYTNVLYLLDLAGIPLRSEERGEEFPLILGGGPCAFNPEPVSPF
                     FDVFLLGEGEEAIVEIVEILRVMKGEKRSDVLSELAKIEGCYVPLLYSVSYHPDGKIK
                     NISPSRVVRKRWVKNLDGAPYPQKIIVPYLNIVHDRIPLEIARGCTRGCRFCQAGMIY
                     RPHRERSPEKVIELAEKLVKSTGYEEVSLLSLSSTDYSAIEYLVRDLSFRLKESKVNV
                     SLPSLRMDAFSVEVAQSLQEVRRSGLTFAPEAGSERLRRVINKGLTDEDIFQTVEKTF
                     SSGWREVKLYFMIGLPGEREEDIRDIANLVKEILGRGKKKGVHLGISAFIPKSHTPFQ
                     WTKQEEIKSLEEKMAILHSLLRPLGRRVRYNWSDFEVNLVEGALSRGDRRISSVIERV
                     YRKGGVMDNWREFFSLRRWQEGFEEEGLDLDFYTHRERDREEIFPWEHLSPGVSKDFL
                     WKEYQLSQEEKITPPCQEGKNCYNCGVC"
     gene            9927..10496
                     /locus_tag="PKN31_02320"
     CDS             9927..10496
                     /locus_tag="PKN31_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOA98798.1"
                     /translation="MDKFRYRVKHQKMAPFHFISQLDLSRLWSRAFRRAGLPVAYSQG
                     FNPRPLLSFGPALPLGVESRAEYWDVFLYRELSPEEILMILNREVLSELKAEEADILP
                     LSFPSISRSTKGVRYSYYFSQSIEEKAGLSPEMGIEEEKREVVGELFVVLFLFKEEKI
                     LYSPAKWAEILRKEWGESPVRIVKEEVLW"
     gene            10513..11994
                     /locus_tag="PKN31_02325"
     CDS             10513..11994
                     /locus_tag="PKN31_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOA98799.1"
                     /translation="MKKEIVIDMSEIETRAALLEEGKVVEFFFDRATQSRLVGNIFKG
                     VVENVLPGIQSAFVNIGLDKNAFLFVGDMLLDDKNKAQKVEELVRSGEEIVVQVTKEP
                     MGTKGARISTKISLPGRYVVLMPGIDTVGISHRIGSAEERERLKKALERLRPVDVGII
                     ARTAAEGVSPEKLKEDIDSLLRLWDRILKKAEIMSAPCALYEEASLVYTLVRDVLDEE
                     VESVWINSFFGYQKIKDFVDSIAPSLSNRVKLFEGRENIFEHFGLNREIEKALSRRVW
                     LKSGGYLVFDRAEAMTVVDVNTGKFVGKGNLQETIFKTNMEAAEEIARQVRLRDIGGI
                     ILVDFIDMEKKEHRRQVVRKLEEALLKDRSRCTVVEMSELGLVEMTRKRLRKEIDSFL
                     REPCSFCEGEGRILSLETIAVNFLRKLEEICSNTSSSTLAFSVGEELGEYLQEEGQKF
                     LDNLKKLYGKEILWKIDPSLAPRRYNIIGVGSEEIREKMEKVI"
     gene            12048..12362
                     /gene="rplU"
                     /locus_tag="PKN31_02330"
     CDS             12048..12362
                     /gene="rplU"
                     /locus_tag="PKN31_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOA98800.1"
                     /translation="MFAIIEAQGKQYKVQEGSIIKLDSWLPQEEEEVVFDRVLMWKKD
                     DALEVGKPYVEGARVTGKVLRRGKDKKVVVFKYKPKVNYRKKTGHRQPITLVRIEKIE
                     GE"
     gene            12367..12627
                     /gene="rpmA"
                     /locus_tag="PKN31_02335"
     CDS             12367..12627
                     /gene="rpmA"
                     /locus_tag="PKN31_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOA98801.1"
                     /translation="MARKKAGGSARNGRDSNSKRLGVKVYGGQFVKAGGIIVRQRGTR
                     IHPGVNVGRGKDDTLFALCDGYVVFEDRKNRKQVSVFSESMS"
     gene            12642..13910
                     /gene="obgE"
                     /locus_tag="PKN31_02340"
     CDS             12642..13910
                     /gene="obgE"
                     /locus_tag="PKN31_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOA98802.1"
                     /translation="MFVDRVVIRVQGGRGGDGAISFRREKYIPRGGPDGGKGGEGGRV
                     FLRASSHRETLRGLAFSPLYQAPSGEGGKGRKQEGRKGEDLVIEVPRGTQVFDNQTGE
                     LIADLLYPGMEVMVARGGRGGKGNVSFASPDNRTPRMAQKGEEGEERELRLELKLFAD
                     VGLVGLPNAGKSTLLSRMSKAKSPVAPYPFSTREPFLGTVGEGEEKFVLLDIPAIVEG
                     ASEGKGLGSEFLRHLERAHLLLYVIDLSSSFSGTNPREDLRILREELRKYNSLLEGKP
                     FLVVANKLDLPGARENWDSFKKTIERERIEGWGISALSGEGVVELLERIREILKEERG
                     KGEAIVAKAEIKEKDISPTIYRFSSTYLEKLLQEADLYPGEEEEFLNRELEKSGFKRY
                     LLSLKSSSVIEVGERKLVWKEKRLKFLADE"
     gene            13903..15033
                     /gene="proB"
                     /locus_tag="PKN31_02345"
     CDS             13903..15033
                     /gene="proB"
                     /locus_tag="PKN31_02345"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOA98803.1"
                     /translation="MNREEFQKNSNLIVVKVGSSLLVDGGALQLDKVRELAHNIARLK
                     EKGKKVILVSSGAVACGMSVMGFTKKRGGIPFKQAMSAIGQSVLMQAYREAFSPLGIQ
                     VAQILLAPEDTHNRVKYLNARNTFEALLHLGVLPVVNENDSVAVAEIKFGDNDRLSAL
                     VASLIGADLLVILSDVEGLFTVDPRLSSQASLVEEVKKIDLEIEEMAGGEGNYFSTGG
                     MKSKIIAARMATLSGIGVIIASGKDFSVLQEIGEGKKRGTFFWPEEKHLPGKKRWIAF
                     AMIPRGSVLIDKGAKKAILDQKSLLPAGVIAVEGNFDLGDCVEILGPSRELVGRGITN
                     YSSEELKKILGLHTDEVREVLGGKDFDEEVVHTDNLVLLGGE"
     gene            15035..16285
                     /locus_tag="PKN31_02350"
     CDS             15035..16285
                     /locus_tag="PKN31_02350"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOA98804.1"
                     /translation="MEKNLKDVARKTKEASYFLANISSFHKDQFLLQLADNLERDEEE
                     IKEENRKDLERAKDMGMKPSLLDRLELSSSRIKAMASGLREVANFPDPVGEVIEGSKR
                     PNGLLITKMRVPLGVLAIIYEARPNVTIDSVGLGIKSGNALLLRGSSSAIHSNQVLVG
                     KTKETLSQCGFPPDVVNLLECKSHEEVQELLTLRDYIDVIIPRGGADLIKLVVENSQV
                     PVIETGVGNCHVYVDGSADWEMAEKIVINAKTQRPSVCNACETLLVDNKIAPQFLPRM
                     IHALEEREVEIRGCEKTRELVKEVKLATEEDWYAEYLDLILAVKVVGGIEEAIKHINS
                     YGSHHSEAIVTQDYTVARKFLEEVDAAAVYVNASTRFTDGGEFGMGAEIGISNQKLHA
                     RGPMGLRELTTVKYMVWGTGQIRE"
     gene            16310..16879
                     /gene="nadD"
                     /locus_tag="PKN31_02355"
     CDS             16310..16879
                     /gene="nadD"
                     /locus_tag="PKN31_02355"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOA98805.1"
                     /translation="MGGTFDPIHHGHLVTAEEVRDYFDLDEVVFVPSARPPHKIGQEV
                     SDPEHRYLMVVLATVTNPYFQVSRAEIERPGPSYSIDTVRYFKDLWGERTEIFFITGA
                     DAFSQISSWNNPEELLELCTFVAASRPGYRLLGVEKKYQGRVKTIEVSALAISSSEIR
                     WRVKRGESIKYLVPETVENYIYKNGLYRN"
     gene            16860..17417
                     /gene="yqeK"
                     /locus_tag="PKN31_02360"
     CDS             16860..17417
                     /gene="yqeK"
                     /locus_tag="PKN31_02360"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOA98806.1"
                     /translation="MDYIEIKDQIIFTLKNMLTEARFYHCLRVAEEAQRLASRWGEEE
                     SKLYIAGLLHDCARDLPYQELIPLLPSYLGEEVYSIPAIFHALAGPAIVDREFGIKDY
                     KIWHAIRWHATSCDMMSRFDKILFISDFCEPGREFPEANLIRKIAEKDWEEAYRRVLK
                     DKLFFLLAHNFPIYSATWKAWNKTK"
     gene            17414..>17832
                     /locus_tag="PKN31_02365"
     CDS             17414..>17832
                     /locus_tag="PKN31_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOA98807.1"
                     /translation="MKTNKKGWKIFYIILGFFLGLFLLFLSTLLGFRIPLRWTVIKEV
                     MGYSEPENAFILVVGQDSIEPRRSDTIILVGINSQIDEILLFSIPRDSRLLVPGRGYD
                     KVNHSYAQGGIGLLRQTLESALGIKIPYFVEVDYEGF"
BASE COUNT         5191 a         2856 c         4561 g         5224 t
ORIGIN      
        1 agattgggat ttgaccgggt gtttgatact aattttgctg ccgacctcac cattattgaa
       61 gaggggacgg aatttatcaa tcgtttgcaa aagggaggga aattacctct cataacttct
      121 tgcagtccag gatggataaa gtttatggag catttttatc ctgagctaat ggagaatgtt
      181 tctacctgta aatctccgca gcaaatgttt ggagcagtag ctaaaactta ctatgctcag
      241 aaattaggaa tagatcctca ggatatggta gtggtttcca ttatgccctg tacggctaaa
      301 aaatttgaat gtcagcggga agagatgaaa tccagtggtt gtcaggatgt agattattct
      361 ctcaccactc gagaagcagc ccgaatgctt aaagaaaaag gtatcgattt aagagagatg
      421 ccggaagaag aatttgatga ccccctgggt atttctaccg gggcagcagc ggtttttggt
      481 gctacaggag gagtgatgga agctgctctt cgctcagttt atgaaatagt tagtggtaaa
      541 actttagaga gtctggattt ctatgatgta cggggaatgg aaggagtgaa ggaagctaca
      601 gttaatattg atggtttaga ggtaggtgta gccgtagctc acagcttagg taatgcccgt
      661 aaaattttgg accaaattaa ggaaggaaac tccaaatacc attttattga gatcatggcc
      721 tgtccggggg gatgcatcgg tggaggaggg caacctatac ccaccgatct ggatattaga
      781 atgaagagaa ttgaggctat ctaccaggta gataaaaatc tttctctgcg aaagtctcat
      841 gaaaacccgg caataaaaca attgtatgag gaatttctgg gtgagcccaa tggagaaaaa
      901 gcccatcaat tgctacatac ccattatctt cctcgggaga aaatgtagaa ctataaaggg
      961 gcagcacccc ttataaccct cgaaaggagg aaaggcaaga aacttaaaaa atttgaatac
     1021 tactgaggaa ggaaaagaat cttactcagt ataggggaac cccccattat taactaaaaa
     1081 gggtgttatt aatatgattt ttgcagttga ctacaattcc gaagaagagg tgattctctc
     1141 catcgacccg ggaaaagaaa aagtaggggt ggcggttctc tcatcgcaag gagaggtctg
     1201 gtttcggaag atattaacgg aagaagaagt agaggaagaa attcagattc tcaagaagca
     1261 gtatggatat tctcaaatag tagtgggaga cagcactggt aaagagaaaa ttctggagat
     1321 cttgagaaaa atgggtctcg cgtttgcttt ggtagatgag cgtaatagct ctgaagaagc
     1381 ccgaaaattg tattttcggg ctcatccccc tcggggttgg agaaagattg ttcccctttc
     1441 ttttctctat cccgatgcgc ctttcgatga ttggcaggca gtggtgatag gaagacgtta
     1501 tcttgatcag aaaaagagtg aaaaaaatgg aagaaaatag tgaattaaga aaagttattc
     1561 cttctttggc ccgtagagct atagaatatt atctgaaaga agggaaatat ctccctcttc
     1621 ccccggggct tccttcttat cttatcagtg agaagcgagg ggtttttgtt tctttgaaaa
     1681 agaaaggaag attacggggc tgtattggca cttatattcc cagatatgaa aatatagcac
     1741 aagaaataat tcataacgca gttagcgctg ccactcaaga cccccgcttt ccccctcttc
     1801 aggaggagga gttgcctttt gtaagtgtgt cggtagatat tttatcttat cccgaagaag
     1861 tgaatagttt agaagaatta gaccctaaaa aatatggagt tatcgtggaa aaaggttgga
     1921 aaagggggtt attgcttccc gacattgagg gagtagatac cgtagatgag caaattcgta
     1981 ttgctctggc taaagcaggg atttcccctc cagagaaatt tactatttat cgctttaccg
     2041 tggataggta tgaggaagaa gattgaaagt agctcgcttt ttcactgact tagaaaataa
     2101 aagagtacaa tgctttctct gtcctcaccg gtgcataatt gctgaaggga gaaatggaac
     2161 ttgccgggtg agaaaaaatg atgggggagt attgtatgct cagacttatg ggaaaatctc
     2221 ttccttcgcc ttagaccctg tagaaaaaaa gcccctctat catttttatc ctggaagaaa
     2281 cattttctca gtagggagtg tggggtgtaa tcttcattgt tctttttgtc agaactggtc
     2341 gatttctcaa actaacccgg ataatttctc tttacgagag ataagtccgg aagatttagc
     2401 tcgccgggct caaagagatg gttctatagg tttatcttat acttataacg agcctttcat
     2461 ctggtgggaa tttgtttatg atacagctca gctgatacga aaagaaggac ttaaaaatgt
     2521 gctggttacc aatggttatg tggaagaaga acctctccga gagttgttac cctggataga
     2581 tgctatgaat gtggacctca aatctatgga tgatgatttt tatcgccgtt actgtggagg
     2641 aactcttcag cctgttttga agaccattga aatttctgtt cgagagggca ctcatgtgga
     2701 gataaccaat ttaataatcc cgggcttaaa tgatcgctgg gatctaattg aaaagctggt
     2761 gagctggatt tatgatttga atcccagtat tcctctgcat ttttcccgct actttccaac
     2821 ttacaaactt gacaatcctt ccactccggt ttctttttta gaagaagttt accattttgc
     2881 tcgggagagg ttaccttttg tttatctggg taatatagga ggaggaaaag gaaatattac
     2941 tttttgtccc acctgtagaa gggaggtggt gataaggaga ggatatcaag tagacacctc
     3001 agggcttttg ggaaatcgtt gtcgcttttg tggttcttct ttgccctttt accatgaaac
     3061 ataaaaaaat attgttagtt tttttgtttt ttattttttt gttctcggga ttttcctttc
     3121 gagataaaga ggaaaagaaa actttttttg gttttgatac ctggatagaa gtggaacttc
     3181 ctcaggatag tgctcttctt ttcccccgga tttcttcttt agttactcat ctggatcaac
     3241 tctggaatcg tttttctcct cggagtatgg tttttaaaat aaaccactct tcctttcctc
     3301 tttcagtgga tgaggatacc tttcgaatat tagaagaagc acaaaaggga ggagaagaaa
     3361 ctcaggggtt tttcaatatc ttgattgctc ctcttttgga tttgtggggt ttccccgggc
     3421 aaccttctat tcctggaaaa gaagaaatag aagaaacagt ggataaaata ggaaagagca
     3481 aactgcatct ggagaaagaa agtaaaagag tatttattga aggagaaggc cagatagatt
     3541 taggtggtat agctaaaggt tacctgatag atgaaatagt ttctctgctg cagaaggaga
     3601 gagtttctca agcattaatc aatgcaggag gagaaatttt tgccctggga aaagaaatta
     3661 aagcaggagt ccgtcatcct cgtgaggaaa aacttctggg ctatattata attaaggatg
     3721 aggcagtatc tacctctgga gattattatc ggttttttga ggaagaaggt gtgcgttatc
     3781 atcatatctt aaatcctttc accggttatc cgggaagaga cttttggagt gtgacagtgg
     3841 ttagcaaaaa ggcaactctt gctgatattc tctccactgt tattatggca ggaggaagag
     3901 aggccctagt agtggtagag aaaaattttc cggagataaa aattttagcg gtagaagagg
     3961 gaggtaacgt ttatctttct ccttctatgg agaaagtttt ttttactgag tgaaaatgaa
     4021 tggaaacaga aaccaaaaac ttttgatact gatagtatta gtggtgatat catctcagat
     4081 tttaatctgg gcagttcgag ctgaggataa taaaatagag aatctccact ctttttttct
     4141 tcctctttat cgagggagtg agtggataaa agatattttt tggggatggg ggaaattttt
     4201 tcaggaccaa aaatctctga gagaagaaaa tcagaagctc aaagaagagt tagagaagct
     4261 tagagctgat aacattttat tacaggagag gatagaggaa ttgaaaataa aaatagaagc
     4321 tggagcaatg gaagaaaagc taccttttga ggtgataccg gctcaggtga taggacgtga
     4381 cccttacgat tggtggggta aagccattat tgatcaagga aaagaagaag gagtgggtga
     4441 ggggttggta gtagttacct accaaggtat ggtgggcaaa atagaagaag cttatcccca
     4501 tcattcagtg gtaagacttc ttttaagccc ttctctatcg gtaggagtct tggttcaaag
     4561 aacccgggat ttaggggtag ttactggaaa cggagaaggt ttgtgcatca tgaaatatat
     4621 ttatcgaact tccgacctta gagaaggaga tatagttata acctcgggtt ggggagaaaa
     4681 tactcctcga ggagttgttg tgggaagggt gtctcagata ggggaggatg atggcagtct
     4741 tttcaaggat gtaatagtag aacccagttg tgatttttct ctattggaca aggtttttgt
     4801 aattaaagct ctctctgaga aattggaatg attgagaaaa ttggtgtctt tttttttctc
     4861 ccccttttat gggagtggat aagttcttcc tttctgttgt atctttggtt tcctcttagc
     4921 catttgcata ttttttcttt tttgatggtt tctttgtttt gggagggaaa agaaagtact
     4981 ctggattttc tccttatttt gcttttagga ataacttctg accttttact tttttccccc
     5041 tggggagcat atctttttat tttgtcgagc cttgctcttt ttacttattt gtgggtgaag
     5101 tttttttcta ccagctttgt ctctctttca gttattttct tctgccttcc tcttttagag
     5161 ttgggcctgg aatgggtagt ggctgctctg ggagaaacga ctttgccctt tcttcctctt
     5221 ttgggagcta aattgctgag cattcctctt aatatttttt tgttttactg gtggcaaaaa
     5281 agagaaacac gccgaggttt agagtatgtg gaatgacgaa atacaggagc gggtgaacca
     5341 gagaattact tatctttctc gcttgttttt aattatactc atacttttat cttttcgatt
     5401 ttggtatctc agtgttttgc aaggggattg gtacaagcaa aaatcagaac aaaaccgagt
     5461 aagaattttt tctcttcctc ccattagagg tagcatttat gatcgcaata atcaagtatt
     5521 agctgaagat gtccctcgct tccgcttgat ttttgaaaaa ggaggagtag aagtggaaga
     5581 aatagaaaaa agagtggaaa gaattttagg aagagagctt tctccccctt ttccggaaag
     5641 agcagtagga gagataatct tggttgacca actgagcatg gaagaggtgg ttaaaataga
     5701 ggaggcccag aatgaattgc ctggagtgat agtggaaagt tatccggttc gtcattatcc
     5761 ttgtggtgaa ccatttgccc atgcagtggg ttacgtagga aagataaatg ccgaggagtt
     5821 taaaatgctg cgctctgaag gctatgagat ggaagacaga ataggaaaga caggagtaga
     5881 gctggaatat gaatctcttc ttcgaggaga aaaaggatac cgaagaatag aagtcaacgc
     5941 tttaggaaag gtagtaggag ttctggatta tagacctcct tactttaaaa attcggtagt
     6001 attgacttta gatagagagc ttcaggaata tagttatgat ttgctgagta ataaaaatgg
     6061 agtgattata gtaggagacc cgtatacggg agagcttttt gctttggtaa gtaaaccgag
     6121 ttttgaccct cagctcttga gctggggaat gagcgctgag gaatggaatg ctttatctca
     6181 aaataaagac aggcctctta ctaatcgccc tcttcaggct ctctatcctc ctggctctct
     6241 ttttaaatta gtgatagcta ctggagcttt agaagaggga ctaacaggca gaaatcggaa
     6301 aattttttgc cctggttttc tagattataa tgaatggcgt tacccttgct ggagaagggg
     6361 ggggcacggg tctgttggta tagaagaggc tattgcccag tcttgtaacg ttacttttta
     6421 taccctgggc ttggaactgg gaccggacaa aattataaac tgggctaata ggctgggagt
     6481 ggctcaacct tcgggcatag atttaccagg agagaagaac ggttttttgc ctactccaga
     6541 atggaagaaa agacagctta aagagatgtg gtatcctggt gatactatta atttgtccat
     6601 cggacagggg tatcttttgg tcactcctct cgaaatgtat cgagtggtga gtagcattgc
     6661 tacccgagga gaagtttaca agcctcatgt acttaaaaaa gtgctaaata gtaaaaggga
     6721 agtagttaga gaggttgctc cggttttaca ggatagaata gagttgagaa atgagacctg
     6781 ggagtctctc atccgaggga tggaaaaagt ggtgaatagt ggaaccggcc gggtttgccg
     6841 aggtttaccg gttaaactgg cagcgaagac tggtacggct caaaatcctc aaggagagga
     6901 tcattcctgg ttttcggggt tttttccttc ttctcatcct caagtagctt ttttagtatt
     6961 gatagaacat ggaggagatg gaagtggtga ggcggcacaa acggctagga aattgattga
     7021 atggtgggaa gaaaatcgag gaggaaaaag tggaaaataa agtggaagat aaagccatgg
     7081 ttttagttct tattgctctt tccctcattg gggtattggc agtatatagt gttgacccct
     7141 tgcgaggaga gggatgggga gaaagcttat ttagtagaca aattatctgg aacttaattg
     7201 gctgggtagc ctttctcttc ttcagtcaga tagactacta tcaattggta aaaagaggat
     7261 ggctctggta ttttttaatg gttttgtccc tgggagcagt tttaattttt ggagaaggcg
     7321 acgaaacagg ttctcgtcgg tggctttttt ctcaatcggt acaaccttca gaaggagcga
     7381 aagtcgccct ggctttattt ctggctcatt atttttctga gcagagggaa agctttgact
     7441 tctggccgct tctcattaaa agtggaatac tttcttttat ccctctggtg cttatttttg
     7501 aggagccgga tttagggtct tctctggttt tactggttat ctggttagga gcgttgtttg
     7561 tttgtggttt tagtgctaag aaatgggcag taataggagg aataggaggg gggcttttac
     7621 ctctttcctg ggttttcttg gaggattacc aaaaagatag gttgctctct tttattgatc
     7681 cagggagaga ccctttaggt agcggatacc atattttaca gtcacaaatt gccattggag
     7741 cagggggttt taccgggcaa ggctggcttt ctggcactca gagtcaactg tatttcttgc
     7801 ctgcccggca cactgatttc atttttgctt cctggtgtga acaactggga tttttaggag
     7861 gaatggtaat aatattttta ctggggctcc tatcctatct ttttctggtt gtagctctcc
     7921 gctctcctaa tttagaagga aagattttag cttctcttct gggaataagc cttatttttc
     7981 agtggacagt gaatataggt ataaacctgg gggttatgcc agttaccggc attgctctgc
     8041 cttttataag ctatggagga agctcgctgg tggtaaattt agcctcggct ggtctggttt
     8101 ataatattag taaaagtgga ggtaaggaaa gctggaaatt gtggatttag aaaaaaccct
     8161 atcacggcag gaaatgctct ctattagtaa accggcacgc tatataggga gagaatggaa
     8221 tataagtctg aaaaaagagg aagacgttga aatatctgtg gctttagctt ttcccgacct
     8281 ttatgagata ggaatgtctc acctgggact gaaaatcctt tactatattc tcaattctct
     8341 tcctggagtc agggcagaga gagcttttgc tccctggccg gatatgggaa aaatgatgaa
     8401 agaaaaaaac attcctcttt actctttaga aaacaaaatt cctcttcgta actttgacgc
     8461 ggtagggata agcttgcaac atgaattgaa ttatactaat gttttatatc ttctggatct
     8521 ggctggcata cctctgcgga gtgaagaacg aggagaagaa tttcctctaa ttttaggagg
     8581 aggaccttgt gcttttaacc ctgaacctgt atctcctttt tttgatgtct ttttgcttgg
     8641 agaaggagaa gaagcgatag tggaaatagt ggaaatatta agggttatga agggtgagaa
     8701 gcgaagcgat gttttgtcag agttagctaa aatagagggt tgttatgttc ccctccttta
     8761 ttcagtttct tatcatcctg atggaaaaat aaaaaatatt tctccttctc gagtggtgag
     8821 aaaaagatgg gtgaaaaatc ttgatggcgc tccttatcct caaaaaatca tagttcctta
     8881 tcttaatatt gtccatgacc ggattcctct ggaaatagct cgaggttgca ctcggggatg
     8941 ccgtttctgc caggcgggaa tgatttatcg tcctcaccgg gagcgttccc ctgagaaggt
     9001 gatagaatta gcagaaaaac tggtaaaaag caccggatat gaagaagttt ctctcctttc
     9061 tttgagcagt actgactatt cagctattga atatctggtt agagatttgt cttttagatt
     9121 aaaagagtct aaagtaaatg tttctcttcc ctcgctgcga atggatgctt tttccgtgga
     9181 agtagctcag tctttgcaag aggtacgtcg cagtggatta accttcgctc ctgaagcggg
     9241 tagcgaaaga ctgcggcgag ttattaataa aggcttgact gatgaggaca tttttcaaac
     9301 ggtagaaaaa actttttctt cgggatggag ggaagtaaaa ctctatttca tgattggtct
     9361 tcccggagaa agggaggagg acataagaga tatcgctaat ttagtaaaag agattttagg
     9421 tcggggaaag aaaaaaggag tgcatctcgg tatctctgcc tttattccta aatctcatac
     9481 tccttttcaa tggacaaaac aggaggagat aaaatctctg gaggaaaaaa tggctattct
     9541 tcattctctt ttacgccctc tgggtcgccg agttcgttat aattggtcag atttcgaggt
     9601 gaatctggta gagggagcgc tgagccgggg agaccggcga atttcttcgg ttatcgaacg
     9661 ggtgtatcga aaaggcggtg tgatggacaa ctggagagag tttttttctc tccggcggtg
     9721 gcaagaaggg tttgaagagg aaggattgga tttagatttt tatactcacc gggaacgaga
     9781 tagagaagaa attttcccgt gggaacatct ttccccggga gtgagtaaag attttctatg
     9841 gaaagaatat cagttatctc aagaagaaaa aataactcct ccctgtcagg aaggaaagaa
     9901 ttgctataat tgtggagttt gctgatatgg ataaatttcg ctatcgagtg aaacatcaaa
     9961 aaatggctcc tttccatttt atttctcagt tagatttgag tcgtctatgg agtagagctt
    10021 tccgccgggc agggcttccg gttgcttatt ctcaaggctt taatcctcgc cctcttcttt
    10081 cttttggtcc ggcgctgccc ttaggggtag agagcagggc agaatactgg gatgtttttc
    10141 tctatcgaga gctatctcca gaggagatat taatgatttt gaatagagaa gtgctttctg
    10201 aactaaaagc cgaggaagca gatattcttc ctctatcttt cccttctatt agccggtcta
    10261 cgaagggagt gcgttactcc tattattttt ctcaatcaat agaggagaaa gcagggttga
    10321 gcccggagat ggggatagaa gaagaaaaaa gagaagtggt tggggaatta tttgtggtat
    10381 tattcctttt taaagaagaa aagatattat atagccccgc gaaatgggca gaaatactaa
    10441 gaaaagaatg gggtgaaagc cctgtaagga tagttaaaga agaggtttta tggtagaacg
    10501 gaggttgaat aattgaagaa agaaatcgta attgatatgt cggagataga aacaagagcg
    10561 gctctcttag aggagggaaa agtagttgaa ttttttttcg atcgagccac tcaatctcgc
    10621 ttggttggca acatcttcaa gggagtagta gaaaatgttc ttcccggcat tcaatcagct
    10681 tttgtgaata ttggtttgga taagaatgct tttcttttcg ttggcgatat gcttctggat
    10741 gataagaata aagcgcagaa ggttgaagag ctggtgcgct ctggcgaaga aatagtagtg
    10801 caagtgacca aagaacctat gggaactaaa ggagcgcgga taagcactaa aatttcttta
    10861 cctgggagat acgtggtttt gatgcccggt atagatacgg tagggatttc ccatcgaata
    10921 gggtcagcag aagagcgaga aagattgaaa aaagcattag aaaggttgag accggtggat
    10981 gtaggcataa tagcacggac agcggcagag ggggtaagtc ctgaaaagct gaaagaggat
    11041 atagatagtc tgcttcgact gtgggataga attttaaaaa aagctgaaat tatgtctgcg
    11101 ccctgtgctc tttatgaaga agccagtctc gtctatactt tggttcggga tgtgctggat
    11161 gaagaagtgg agagtgtctg gattaattct ttttttggtt accagaagat aaaggacttt
    11221 gtggattcca tcgctccctc tttgagcaac cgggttaagc tttttgaagg tcgggagaat
    11281 atatttgagc acttcgggct taatcgagaa atagagaagg ctttaagccg gagagtttgg
    11341 ttgaaaagtg gaggatattt agtttttgat cgagctgaag ctatgaccgt tgtggatgta
    11401 aacactggta agtttgtggg aaagggaaac ttgcaagaga ccatttttaa gaccaatatg
    11461 gaggcggcag aagaaattgc ccgtcaggtg agattgcgag atataggagg tataattctc
    11521 gttgatttta ttgatatgga aaaaaaggaa caccgccgac aggtggtgag aaaattagag
    11581 gaagccctct tgaaggatag aagccgctgc acagtggtag agatgagtga attgggtctg
    11641 gtagaaatga ccagaaaaag attgagaaaa gagatagatt cctttttacg agagccttgt
    11701 tctttctgcg agggagaggg aagaatattg tctctggaaa ctatagcagt taattttttg
    11761 cgaaagctgg aggaaatatg cagcaatacc tcttcttcta cactggcatt ttcagtagga
    11821 gaggaattgg gagagtatct ccaggaagag gggcaaaagt ttctggacaa tttaaaaaaa
    11881 ttatacggta aggaaatact gtggaaaata gacccatctc ttgctcctcg ccggtataac
    11941 atcatcgggg ttggcagtga agagattcgg gaaaaaatgg agaaggttat ttgactaaat
    12001 tccaatttct atggtaaatt ctttatcgtt ctctggaggt gaggatcatg tttgctatta
    12061 ttgaggcgca gggaaaacag tacaaggtgc aagaaggaag tataattaag ctggatagct
    12121 ggttgccaca ggaagaggaa gaggtggttt ttgatcgggt gcttatgtgg aaaaaagatg
    12181 atgcactgga agtgggaaag ccttacgtag aaggagcaag agtaaccgga aaagttctta
    12241 gaagaggcaa ggataaaaag gtagtggtgt ttaaatataa acctaaggtg aattatcgga
    12301 aaaaaaccgg tcatcgtcag cctattactt tggtgcgtat tgaaaaaata gaaggggagt
    12361 gaaactatgg ctcgtaaaaa agcgggagga agcgcgcgta atggtagaga tagtaattcc
    12421 aaacgtttgg gagtaaaggt ttatggcgga caattcgtta aagccggggg gattattgtt
    12481 cgccagcgag gcactagaat tcatcctgga gttaatgtag gtagaggtaa agacgatact
    12541 ctttttgctc tctgtgatgg atatgtggtt tttgaggata ggaaaaatag aaagcaggtc
    12601 tccgtttttt ctgaaagtat gagttaaggc gaggagagta attgtttgta gatagagttg
    12661 ttatcagggt gcagggaggc aggggaggag acggagcgat tagctttcgg cgggagaaat
    12721 atattcctcg gggcgggcca gatggcggaa aaggaggaga aggaggaaga gtttttctcc
    12781 gtgcctcttc tcacagagag actctccggg gtttagcttt ttctccttta taccaggctc
    12841 cttctggaga aggaggtaag ggtagaaagc aagaaggtag aaaaggcgaa gatttagtga
    12901 ttgaggttcc ccgaggaacg caggtttttg ataaccagac aggagaatta attgccgacc
    12961 ttctttatcc gggaatggaa gtaatggttg cccgaggagg aagaggaggg aaagggaacg
    13021 tttcttttgc ttctcccgat aatcggactc ctcgaatggc gcaaaaagga gaagaggggg
    13081 aggagcgaga gcttcgcctg gaattaaagt tgtttgccga tgtgggttta gtgggattgc
    13141 ctaatgctgg taaatccact ctcctttccc gaatgagcaa agcgaagtct cccgtagctc
    13201 cttatccttt ctctactcgg gaaccatttt tagggacagt gggcgaaggt gaagaaaaat
    13261 ttgttttgct ggatatcccg gcaattgtag agggagcaag cgagggaaag ggattaggga
    13321 gtgaatttct tcgccattta gagagggcac atctcctttt atatgtaatt gatttatctt
    13381 cttctttttc gggaactaat ccccgggaag atttaaggat tttacgggag gagttaagaa
    13441 aatataattc tcttcttgag gggaaacctt ttcttgtagt ggctaacaag ttagaccttc
    13501 ccggagctcg agaaaattgg gatagcttta aaaaaaccat agaaagagag agaatagagg
    13561 gatggggaat atcagctctc tcgggggaag gagtggttga gcttctcgag cgaataagag
    13621 aaatattaaa agaggagaga gggaaagggg aggcaatagt agcaaaggcg gaaataaaag
    13681 aaaaagatat ttctccaacg atttatcgtt tttcttccac ctatttggag aagctgcttc
    13741 aggaggcaga cctttacccc ggagaggaag aggagttttt aaatcgggaa ttagaaaagt
    13801 ccgggtttaa gcgttattta ttatctttaa aatcctcaag tgtaatagaa gtgggagaaa
    13861 gaaagttagt ttggaaggag aaaaggttga agtttttagc tgatgaatag agaagagttt
    13921 caaaaaaata gcaatttgat agtagtaaaa gtggggtcct cgcttcttgt ggatgggggt
    13981 gctttacaat tagataaagt cagagaatta gctcataata tagcccgctt aaaagaaaaa
    14041 gggaaaaaag ttattctggt ttcctctgga gcagtagcct gcgggatgag cgttatgggt
    14101 tttactaaaa agcgaggagg cattccgttt aaacaagcta tgtcagctat cggacaaagc
    14161 gttttgatgc aagcttaccg cgaagctttc tccccattgg ggatacaggt agctcagata
    14221 cttctggcac ctgaggacac tcataatcga gttaaatacc tgaatgcccg taatactttt
    14281 gaagcccttc tccatttggg agttcttcct gtggttaatg aaaacgactc ggtggcggta
    14341 gcagaaatca agtttggcga caacgatcgt ctttcagctc tggtggcttc cttaataggt
    14401 gcagaccttt tggttattct ctctgatgta gaaggccttt ttactgttga ccctcgtctt
    14461 tcgtctcagg cctcgttagt ggaggaagta aaaaagatcg atctggagat agaagaaatg
    14521 gccgggggag aaggtaatta cttttctacc ggggggatga agagtaaaat aatagctgct
    14581 cgaatggcca ctctttccgg gataggggtg ataatcgctt cgggtaaaga tttctcggtt
    14641 ctgcaagaga ttggagaagg aaaaaaacgg ggcactttct tctggcccga agaaaagcat
    14701 cttccgggaa agaaaaggtg gattgctttt gccatgatac cccggggtag tgttttgata
    14761 gacaaaggag ctaaaaaggc tattttagat cagaagagtc tccttcctgc tggagtgatt
    14821 gcagtagaag gaaactttga tctgggagat tgcgtagaaa ttttaggtcc ctctcgagaa
    14881 ttggtgggaa gaggaataac taattattct tcggaggagc taaaaaaaat attaggcttg
    14941 catactgatg aagtgaggga agttttagga gggaaagatt ttgatgaaga agtggttcat
    15001 actgataact tagtacttct gggaggagag tgatatggaa aaaaacttga aagatgttgc
    15061 ccggaaaact aaagaggctt cctattttct tgctaatatt tcttctttcc ataaagatca
    15121 gtttctttta cagctggctg ataatttaga aagagatgaa gaagagataa aagaggaaaa
    15181 tagaaaagat ttagaaaggg ctaaagacat gggaatgaag ccttcgcttc tggatcgtct
    15241 ggaactgtct tcttcccgca taaaagccat ggcttcaggt ctgcgggaag tagctaactt
    15301 tcctgatccg gtgggggaag tgatagaagg ctctaaaaga cccaatggcc ttttgatcac
    15361 caaaatgagg gtgcccttgg gtgtgttagc tattatttat gaggctcgtc ctaatgtaac
    15421 gatagatagc gtgggtctgg gtataaaatc gggtaacgct cttcttcttc gaggtagtag
    15481 ctcagccatt cattccaatc aagttctggt gggtaaaacc aaagagactt tatctcaatg
    15541 tggttttccg cctgatgtgg ttaatttgct ggagtgtaag agccatgaag aagtgcaaga
    15601 acttttaact ctccgggatt atatagatgt gattattccc cggggaggag ctgatttaat
    15661 aaaattggtg gtagaaaatt ctcaagttcc agtaatagaa acaggggtag gaaattgcca
    15721 tgtctatgtg gatgggagtg ctgattggga aatggcagaa aaaatagtta ttaatgctaa
    15781 aacccagcgt cccagtgttt gcaatgcctg cgaaactctt ttagtggata ataaaattgc
    15841 tcctcaattt cttccccgga tgattcatgc tctggaggaa agagaagtag aaataagagg
    15901 gtgtgaaaaa acgagagaat tagtgaaaga agttaaatta gcaacggaag aagactggta
    15961 tgcagaatac ctggatttaa ttttggcagt taaagtggtg ggaggcatag aggaagctat
    16021 aaaacacata aatagctatg gttctcatca ctcagaagcc atcgtgactc aggattatac
    16081 ggtagcaaga aagtttctgg aagaggtgga tgctgcggcg gtttacgtca atgcctctac
    16141 tcgttttacc gatggaggag aatttggaat gggagcagaa attggtataa gcaatcaaaa
    16201 gctccatgct cgaggcccta tgggcttgag agagcttact actgttaaat atatggtttg
    16261 gggaacagga caaatacggg agtgatattc tacggataaa aaaggaatta tgggaggaac
    16321 ttttgatcct atccatcacg gccatttggt tactgcagag gaagtgagag actactttga
    16381 cttagatgaa gtagttttcg ttccttctgc tcgtcctcct cacaaaatag ggcaagaagt
    16441 gagtgacccc gagcatcgct accttatggt tgttttagct actgttacca atccttattt
    16501 tcaagtttct cgggctgaga tagagagacc tggtccttct tattccatag atacagttcg
    16561 ttactttaaa gatttatggg gagagagaac agagattttt tttataaccg gagctgacgc
    16621 tttttctcag atttccagct ggaataatcc ggaagaattg ctggaactct gtacttttgt
    16681 agctgcttct cgtccgggtt accgattgtt aggggtggag aagaaatacc aaggaagggt
    16741 aaaaactatc gaggtctctg ctttagctat ttcttcttct gagatacgat ggagagtaaa
    16801 aagaggcgaa tcaatcaagt atctagtacc agaaacagta gagaattata tttacaaaaa
    16861 tggattatat agaaattaaa gatcaaataa tttttacttt aaagaatatg cttaccgagg
    16921 cccgatttta tcactgtttg cgagtagcag aagaagctca acgtttagct tcccgctggg
    16981 gagaagagga gagcaagcta tacattgccg gtcttttaca tgattgtgct cgagatttac
    17041 cctatcagga attgattcct cttttacctt cttatctggg agaggaggtt tattctattc
    17101 ccgctatttt tcatgcttta gccgggcctg cgatagtaga tagggaattt ggaattaaag
    17161 actataaaat ctggcatgcc atacgctggc atgctacgtc ctgtgatatg atgtccaggt
    17221 ttgataaaat tttatttatt tctgattttt gtgaacctgg tcgagaattt cctgaagcaa
    17281 atttgataag aaagatagca gaaaaagact gggaggaggc ttaccggcgg gttttgaagg
    17341 ataaattatt ttttctcctt gctcataatt ttccaattta ttccgctacc tggaaggcct
    17401 ggaataaaac aaaatgaaaa ctaataaaaa aggctggaaa atcttttata ttatcttggg
    17461 ctttttttta ggactttttc ttcttttttt atctactctt ctgggattca ggatacctct
    17521 gcgctggacg gtaataaagg aagtgatggg ttatagtgaa ccagaaaatg ctttcatttt
    17581 agtagtggga caggatagta ttgagccccg gaggagtgat actataattt tagtgggtat
    17641 taattcccag atagatgaaa tccttctttt ctctattcct cgggattctc gtcttttggt
    17701 acccggaagg ggatatgata aagtcaatca ttcttatgct caagggggga tagggcttct
    17761 ccgtcaaacc ttggagagcg ctctggggat aaaaattccc tattttgtgg aggtagatta
    17821 tgaagggttt ga
//
LOCUS       DAOPSS010000020        17679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2694_length_17679_cov_8.737574, whole genome shotgun sequence.
ACCESSION   DAOPSS010000020 DAOPSS010000000
VERSION     DAOPSS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 17679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17679
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2694_length_17679_cov_8.737574"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2308
                     /gene="mfd"
                     /locus_tag="PKN31_02370"
     CDS             <1..2308
                     /gene="mfd"
                     /locus_tag="PKN31_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOA98808.1"
                     /translation="ERIPSFWGKIDDLKDYLETSLWNKIFLLLPPEKLEILSSLLEEE
                     KVDFGLNLEENKKVILFPGYLREGFGIPEIGLAFISSWEIFGKEFVPSPEVPKKSLVS
                     LSSLSPGDYVVHEEEGIGIFRGWKEMTVEGIKRVYVEIEYGRGDILYLPADKIYLLQK
                     YVGAGEEKPRINRLGKDEWARTKERAKRAAEKVAQDLVEVYARRQLEEGYSFSPDTPW
                     QKELELSFPFLETPDQKKAIEEVKKDMESPRPMDRLICGDAGYGKTEIAIRAAFKAVM
                     DGKQVAVLAPTTLLSEQHYVTFRERMKDFPVTIAVLNRFRTPQEQKSILKKVEKGEVD
                     IIIGTHRLLQKDVFFKDLGLLIVDEEQRLGVRHKEQLKKLKSNLDVLTLTATPIPRTL
                     YLSLLGLRDISVVETPPEARKPVYTLVMPRDKKVIKEALEREIEREGQVFYVCPRIRN
                     LMPVKEELDDLLPGVKVALAHGRMGGKELEKVMEDFYRGKISVLLCTTIVGIGLDVPN
                     ANTIIVDPATLFGLAQLYQLRGRVGRFDREAYAYFLYPPRLTGEARERLEALLEFGAT
                     GSGMKLALRDLEIRGAGNILGMEQHGFIQEVGFSLYTRLLEEEIARLRGEEEISSITP
                     HIDLKEEAYLPSFYLSRDSERFYFYQKLLGVRKEKEIEEIKEELEDRFGRLPRQAENL
                     LQITKIRHYAKIAQIESIEEKDGELYFVASLPVLLGLSQYMEERGKEGKLFNYQGREA
                     FKTKMLPLQDLVSLLEGMVIESVQWQSR"
     gene            2286..3083
                     /gene="mazG"
                     /locus_tag="PKN31_02375"
     CDS             2286..3083
                     /gene="mazG"
                     /locus_tag="PKN31_02375"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOA98809.1"
                     /translation="MSSGRAGELFEELIEVVAALRGEGGCPWDREQTRETLKPLMVEE
                     LYEALDAIDKKDEVRLKEELGDMLLHIVFHAQIGKENHTFTIEEVLEHLIYKMKRRHP
                     HVFGEAKVKDVDGVLLNWEKIKETERDKSGMLSTLPRSLPALLRAQAMQERASRVGFD
                     WEAPEEVEKKVEEEWGEFREAWKSKNPQKMKEEWGDLVFALVNLARHLNIQAEEALQG
                     ANNRFAERFGFIERKIKEQGRELSGATLEEMDALWEKAKEKFTPEEK"
     gene            3041..3478
                     /locus_tag="PKN31_02380"
     CDS             3041..3478
                     /locus_tag="PKN31_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98810.1"
                     /translation="MGKSEREIHAGGKISLAEFLKRLKVILPLVQGWNPDLLIAVNPE
                     GLILSGFLASFLDKEVRNVAFKEEPFQITWDNLGNLEGKRTVLVAGRFREEKSREEIE
                     KWVKDHGALSVLVMVAVGGKGDYSLFPEWGENILLPWEDNEVR"
     gene            3465..3716
                     /locus_tag="PKN31_02385"
     CDS             3465..3716
                     /locus_tag="PKN31_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOA98811.1"
                     /translation="MRLDKFLKSSSLIKRRPLAQAACKGGRVLVNGKVAKPGTGLKLE
                     DEITIFTSAYKIVVRVKSLSEKERGEELYEVIKKERLEE"
     gene            3713..4861
                     /locus_tag="PKN31_02390"
     CDS             3713..4861
                     /locus_tag="PKN31_02390"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOA98812.1"
                     /translation="MRILVAPDSFKGSLKSTEVASLVKKGLQRVDKGWLVVERPLADG
                     GEGTVEVLVQGTGGKIIEKEVSGPRGEKVKAHIGVVSCSCGEVAVLEMAQVAGLTLLP
                     PEERNPRFTTTYGVGELIKEAVNLGFRRIVLGVGGSATNDGGMGALRALGVRFFDREG
                     KELKGVGDDLLLVSSIDSQHLFEKPEEVELVIACDVKNPLYGEEGAAYVYGPQKGANP
                     EEVEALDQGLRNYARLVREHTGIEIDHLPGAGAAGGIGAGLYAFWGAQFASGIQLIMD
                     ILHLEEEIKKADLVISGEGKIDKQSFYGKVVSGVEEKCRKWGKPLLLVGGKISEEAFS
                     LWEERVLALFSIANGPITEEEAFQKAPFLLEHLAFNLGRVWKGGEIYA"
     gene            4854..5255
                     /locus_tag="PKN31_02395"
     CDS             4854..5255
                     /locus_tag="PKN31_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HOA98813.1"
                     /translation="MPEKIEFSFPERGITIEAELSDSSLAEEIFRILPVEARVSRWGK
                     EIYFAIPLKAPIVSPQEVVEEGDIAYWPDGACLCLFFGPTPVSKNGEIRPASAVEVVG
                     KILSPWAILDEIPSGDIVKITQFDRKGVDKG"
     gene            5252..6538
                     /gene="eno"
                     /locus_tag="PKN31_02400"
     CDS             5252..6538
                     /gene="eno"
                     /locus_tag="PKN31_02400"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOA98814.1"
                     /translation="MSTIFDVHAREILDSRGNPTVEVDVILASGAFGRASVPSGASTG
                     TFEAVELRDGDKRRYLGKGVRKAVENVNEIIAPEIIGLDALDQAVVDKTLIELDGTPN
                     KAKLGANAILGVSLAVARAAADYSGLSLYRYFGGANARELPVPLMNILNGGKHADSGV
                     DIQEFMIVPCGARSFGESLRMGAEVFHNLARVLKRSGYSTGVGDEGGFAPNLHSSEAA
                     IEVILEAIQLAGYEPGKDVYLALDVAASELYKDGIYVFEREGVRRNVEEMIDFYRRLV
                     ENYPVISIEDGLAEEDWEGWKKLTFALGDKVQLVGDDLFVTNKERLLRGIREKCCNSI
                     LIKLNQIGTLTETLETIEIAKRAGYTSVISHRSGETEDSTIADLAVGCNAGQIKTGSL
                     CRSERTAKYNQLLRIEEELDNAAIFRGKEVFKVSGY"
     gene            6573..6827
                     /locus_tag="PKN31_02405"
     CDS             6573..6827
                     /locus_tag="PKN31_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOA98815.1"
                     /translation="MALSLFSFNLRLSNKLVDYFDLAREMKELEKRESALSEEVERLE
                     KEREYLDEDWYVEKLARERLQLVKPGEIVVKVINPESPEH"
     gene            6872..6949
                     /locus_tag="PKN31_02410"
     tRNA            6872..6949
                     /locus_tag="PKN31_02410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6907..6909,aa:Pro,seq:ggg)
     gene            6991..7773
                     /locus_tag="PKN31_02415"
     CDS             6991..7773
                     /locus_tag="PKN31_02415"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOA98816.1"
                     /translation="MPSNVKDLFSLQGEVAIVTGGAQGLGEQMSIALGEAGAQVVVAD
                     LNLEKAQKVASHIQSLGVDSLALCVDVTKKGEVGEMVEKTLERFGKIDILVTSAGVGQ
                     WMKAEEMPEEDWKKVLDVNLNGVFLCCQAVGREMIKRKKGSIINISSMSGLVVNTPQA
                     QSHYNSSKAGVIMLTKSLASEWAPYNIRVNTIAPGYMETQLVGDLLKEYPDYADLWKK
                     LTPFGRLGNPEEIKGPCVFLASPASSYVTGSVLVMDGGYTIW"
     gene            7863..9290
                     /locus_tag="PKN31_02420"
     CDS             7863..9290
                     /locus_tag="PKN31_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOA98817.1"
                     /translation="MRRRLLTVVFVLCFLLVPTMVFAQVANPDWWKKAAEPYKGVVIH
                     GVSESTPPSKAIAEIAVPAFEELTGIKVELETTSWDEMYNKEIQDMQAGAGIYDFVYL
                     EQDAVYSYIEQDWLTDLTPFVDNADLTEPDFDLPDFTSFIDYFKGKDGELYGIPFEAF
                     LKTYIYRTDLFNDSDIQAAFKEKYGYDLAVPTTWEQYEDIAKFFTEYGKEKGIELYGH
                     VAQAKTHPCLAYELCETIWPPWGVYNWGINLENMRTSVENGGTLNSDTAKEALGWYVD
                     MLQYAPPGVRTYTWDEAAAVFGTGIIAQGFLYMENLGWLAHDPDRSVVIGKVAIDIPP
                     AKPGVMEAAEAGEGYIGYYDGGAWGIPYCSKNKEAAWLFIQWCTRKEWQPEFAELATT
                     VVRKSAFEDPIIDELNVAMGGYYTVMQEKGHLFAGCPPLPMQTPLNELYLQWISRAVA
                     GEVSPEECLDNLAKEADELMEELGY"
     gene            9303..10172
                     /locus_tag="PKN31_02425"
     CDS             9303..10172
                     /locus_tag="PKN31_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOA98818.1"
                     /translation="MRVKKTQVWGLISPTLILMILIGVIPVIYVIYLSVFKYSVFSKT
                     GMVYVGFDNFRKLVFDASFLKSLSLGLTFVAICCLVEIPLGLLIANVLTYEFKGKGVF
                     RTIMALPLAMAPVSIGSMWVLLTNPDVGPLPYFLGKIGVDYNIGVNATQAFATTVFMD
                     VWHWTPFVILTFMAGLASLPKDPYEASLVDGANRWQTFRYITLPLLRPVILTTLFIRI
                     MDTFRVFDEVWMLTSGGPGTATRFVSIHLVREVLRSMNYGYSSAMSLFLLYLTIVICW
                     LLLTFISISQEEV"
     gene            10173..11003
                     /locus_tag="PKN31_02430"
     CDS             10173..11003
                     /locus_tag="PKN31_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOA98819.1"
                     /translation="MKISKKGLRLFFIYVLCILIAIATLFPIYWTFIISAQSKLEIFS
                     GPNFFPSTFHSENYTRPFLQDVYGRYLANSLIVATGNTLLVIVLAVMSTYAFSRFQIP
                     GSSNIFFWTITNRMAPAAVFIVPFFLIFSRLGLRDTRIGLIILYCIFNLPFAIWLLKG
                     MMDSIPKELDEAAMIDGCSIWGVLVRVIIPLAKPGIMVTAILTWIFAWNEYLFASILT
                     NVKARTITTGLIEFVTVIGTNWGEMAAVSMVCLIPAVVFIGIAQQHIITGLTFGAVKD
                     "
     gene            11013..11183
                     /locus_tag="PKN31_02435"
     CDS             11013..11183
                     /locus_tag="PKN31_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98820.1"
                     /translation="MRILPMKTNLFDRIFIGAVVMFGLHLFWVRFVEQFIPLYVATIG
                     SLIFLVTLIITG"
     gene            complement(11354..12763)
                     /locus_tag="PKN31_02440"
     CDS             complement(11354..12763)
                     /locus_tag="PKN31_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOA98821.1"
                     /translation="MAQTFGLIVGNRGFFPDELAKEGRSKILEVLEKKGFEVVAPSTE
                     DTKFGTIETWEDAQKCARLFDENRHRIEGIIITLPNFGDERAAAQTIRLSGLNVPVLV
                     HAFPDQINTLDVQNRRDSFCGKLSICNNLKQFQIPFSLTSYHTEDPDSSIFGEDLEWF
                     AGVCRVVKGFKNLKAGAVGARPAAFNTVRFSEKILESYGISVETIDLSEIIGNMNRLD
                     NNNLKVKGKLQEIKDYIDTAGIPEEALVKMAKLLVVLEEWIEATGVSLFSFQCWNSIE
                     ENYGIMPCTVMSMFSEKLIPAACEVDLVGGLSMYALQLASQKPSALVDWNNNFGDDPD
                     KAILFHCSNFPKSLLQGARMGYGDIISSSVSKEKAYGTCYGTIPNGELTLARLATNDE
                     EGNLISYIAEGEITPDKVNTFGGVGVAKIEDLQDLLKYICINGFEHHVAISLSQSGDI
                     LSEAFENYLRIETYYHNIY"
     gene            12885..13724
                     /locus_tag="PKN31_02445"
     CDS             12885..13724
                     /locus_tag="PKN31_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOA98822.1"
                     /translation="MKKVLLSSLVLCFILSLSGLSWGVEKVDVQGSTTILPIMQIIAE
                     EFMAQNEDIEVTVGGGGSGVGISALLDGVTNVAMASRDMKEEEWQKAKEKGLDIREIE
                     IAKDALSVIVNYLNPISEIDVDTLQKIYTGGISNWQELGVALDGEIVIISRDTNSGTF
                     EVFNEHVLRGEELADKALRLPSNQAVLNEVVSNPNAMGYVGLGYAQQGGDKIKALTVN
                     GVEASPEKALSGEYPLSRGLYLYVNGEPQGAVKKLIDFVLGPEGQTIVEEEGFIPLSL
                     NED"
     gene            13841..14527
                     /locus_tag="PKN31_02450"
     CDS             13841..14527
                     /locus_tag="PKN31_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA98823.1"
                     /translation="MGLDIVLIEDEKDVVDFVTYFLQKEGYQVFPFLRGEDFLEKMDT
                     LLPDVIILDLMLPGIDGLEVFRYIREYPRFRNVPIIMLTAKSDPLDVVLGLEIGADDY
                     VTKPFHPRELLARIKAVVRRTSSPSFSEKEVLKIGDFWLDSDHLMVKENGKEIYLSWR
                     EFRLFELLIKNRGKVISRQQILDSIWGEDSFITDRVVDVYIANLRKKLGSSGNKIVTV
                     RGVGYKFEST"
     gene            14622..15641
                     /locus_tag="PKN31_02455"
     CDS             14622..15641
                     /locus_tag="PKN31_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA98824.1"
                     /translation="MLLERENAEQVLSLKENILQAMDSLSLAMAFYDGQKEKVMFNSS
                     FGKFIGGKEKGEAFLWEIPLLGTWLRKEESLQVIEVGSLKLHLRIWGGENDKVVFLED
                     IGEKERKSREMNYLSTALWHELRTPLTALEGNVVILEEEVKDQFLLDISYKMEQQINR
                     IETTLDNLRQLTSIFQEENQFSTAGELKRTLEEVINNFEDDIRRLDLSLSVDTSLLDK
                     VKAIFPFSSADFLILFSNLFSNALKFNYPKGRAEIKLNREEEGVFLELSNTTSNTSRE
                     FIKWLFEPSSTFSPRGADGRGVGLYLAKEAVARMGGRISAHYGEGDSVIFRVLLCVED
                     KNEEN"
     gene            complement(15810..16676)
                     /locus_tag="PKN31_02460"
     CDS             complement(15810..16676)
                     /locus_tag="PKN31_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HOA98825.1"
                     /translation="MPLVSTQTILKLAEEKNFALPAFNVPIPEFILWVLEEGEEMRAP
                     LIIQVAPVEYEALDIRMFYGTLLFLSQKFSIPFSLHLDHAHHLEDILLAIQNGFSSVM
                     IDGSRLPLEENINLTNKVIEIAHPVEVLVEGELGRVGGLEGDESWEEKSKEEDFFTSP
                     QEAQEFTGKAPVDMLAVAVGTRHGLYKGRTPQLQFERIKEIRAKTSLPLVIHGGSGTP
                     REQLRKAAQLGVRKINFSTALRVQYLSAFQEYLANHPEELMVGKILQGIEGKVKEAIR
                     ECIIASCAEGKV"
     gene            complement(16889..>17679)
                     /locus_tag="PKN31_02465"
     CDS             complement(16889..>17679)
                     /locus_tag="PKN31_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA98826.1"
                     /translation="LGHSEEIIAKALRGMSEKPIIATKCGLVWNEKGEVFGCLKRDSI
                     RKEVEDSLRRLEVEVIDLYQIHWPDPDPDIEEAWSTMADLVKEGKIRYAGVSNFNVEQ
                     MKRIEKIHPIASLQPPYSMIMRDIEKEILPYCAEKNMGVIVYSPMQKGLLTGKFTPER
                     VKNLPPDDHRRSDPQFNEPLLSINLELVEKLKPIAEKHGRTLAQLAIAWVLRRPEVTA
                     AIVGTRRPSQIEETFPAGDWELAAEDIEAIEKLLQEREEKIKNL"
BASE COUNT         4962 a         3109 c         4300 g         5308 t
ORIGIN      
        1 agagcgaata ccttcttttt ggggcaagat tgacgatttg aaagattatc tggaaacaag
       61 cctctggaat aaaatttttc tccttcttcc tccggaaaag ctggagattc tttcttctct
      121 tttagaagag gagaaagtgg attttggctt aaacctggaa gaaaataaaa aggtgattct
      181 ctttcctgga tacctgaggg agggttttgg gataccagag attggtttag ctttcatctc
      241 ttcctgggaa atttttggga aagaatttgt tccctctccg gaggttccca agaaatctct
      301 ggtctctctt tcctctcttt ctcccggaga ttatgtggtg catgaggaag aaggcattgg
      361 tatttttcga ggatggaaag agatgactgt agaggggata aaacgagtgt atgtggagat
      421 agaatacggt agaggagaca ttctttacct tcctgcagat aaaatctatc tcctgcaaaa
      481 atatgtgggg gcgggagagg agaagccccg aattaatcgt ctggggaaag atgaatgggc
      541 gaggaccaaa gaacgggcta aaagagcagc agaaaaagta gctcaagact tagtagaagt
      601 ttatgctcgg cgtcaattgg aggaaggtta tagtttttct ccggatactc cctggcaaaa
      661 agagctggag ctatctttcc cttttctgga gactccggac cagaaaaaag ctattgaaga
      721 agttaaaaaa gatatggaaa gccctcgccc catggaccgc ttgatctgtg gagatgcggg
      781 atacgggaaa acagagattg ccatacgggc agctttcaaa gcagtaatgg atggaaagca
      841 agtagctgtt ttagctccca ctactcttct ttctgaacaa cactatgtta cctttcggga
      901 aagaatgaaa gactttccag tgactattgc cgttttaaac cgctttcgta ctccccagga
      961 gcaaaaatca atactgaaaa aagtggaaaa gggagaagtg gacatcataa tcggcactca
     1021 tcgccttttg caaaaagatg tattttttaa agatttaggt ttattgattg tagatgagga
     1081 acaacgctta ggggtgaggc ataaagagca gctgaaaaaa ctcaagtcca acctggatgt
     1141 tttaaccctc actgccactc ctattcctcg tactctttat ctttctcttt taggtcttcg
     1201 ggatatcagt gtggtagaaa caccaccgga agcgagaaaa ccggtttata ctctggtgat
     1261 gcctcgagat aaaaaagtga ttaaagaagc cctggagaga gagatagagc gagaaggtca
     1321 ggttttttat gtctgccctc ggattcggaa tttaatgccg gtgaaagaag agctggatga
     1381 cctacttccc ggggtaaaag tggccttagc tcatggaagg atgggaggta aggagttaga
     1441 aaaagttatg gaagattttt atcggggtaa aatttctgtt ttactttgta ccactattgt
     1501 aggaattggt ctggatgtcc ccaatgccaa cactatcatt gtagatccgg ctaccctgtt
     1561 tggattggct cagctttatc agttgcgggg aagagtgggg aggtttgata gagaagccta
     1621 tgcctatttt ttgtatcccc ctcgtctaac tggagaggcc agggagagac tggaagctct
     1681 tcttgagttt ggtgccaccg gttcaggtat gaaactggct ctccgggatt tggaaattcg
     1741 gggagctggt aatattctgg gaatggagca gcacggtttt attcaggaag tgggtttttc
     1801 tctttacact cgtcttttag aggaagaaat tgctcgcctg cgaggagaag aagagatttc
     1861 ttccattacc cctcatatag atttaaaaga agaggcttat ctaccttctt tttacctgtc
     1921 cagggatagt gaaaggtttt atttctacca gaaactgctg ggggtgagaa aagaaaagga
     1981 aatagaggaa ataaaggaag aattggaaga tcgctttgga cgtcttcccc ggcaggctga
     2041 gaatcttttg cagattacaa aaataaggca ttatgctaaa atagctcaga ttgaaagcat
     2101 agaagaaaaa gatggagaac tttattttgt ggcttctctt ccggttcttt tgggtttgag
     2161 ccagtatatg gaagagaggg gaaaagaagg aaagctattc aactatcaag gcagagaggc
     2221 attcaaaact aaaatgttgc ctctccagga tttagtttca cttctggaag ggatggttat
     2281 tgagagtgtc cagtggcaga gccggtgagc tttttgagga attaatagaa gtagtggctg
     2341 ctttacgggg agagggaggc tgtccctggg acagggaaca aactcgggag acactaaaac
     2401 ctctcatggt ggaagagctc tatgaagcct tagatgccat agataaaaaa gatgaggtga
     2461 gattgaagga agaattaggg gatatgctgc tgcatatcgt ttttcacgct cagataggaa
     2521 aggaaaacca tacctttact attgaggagg tgttagaaca ccttatttat aagatgaaga
     2581 gacgccatcc tcatgttttt ggagaagcta aagtaaagga tgtagatggg gtgctcttaa
     2641 actgggaaaa aataaaggaa acagaaagag ataaaagcgg gatgctttcc actttgcctc
     2701 gcagtcttcc tgctcttttg cgagctcagg ctatgcaaga gcgagcctct cgagtgggtt
     2761 ttgattggga agcacctgag gaagtagaaa aaaaggtaga agaagagtgg ggagaatttc
     2821 gagaagcctg gaaaagcaaa aaccctcaga aaatgaaaga ggaatgggga gatctggttt
     2881 ttgctttagt taacttagct cgtcatctga atattcaggc tgaagaagct ctgcagggag
     2941 ctaataatcg ttttgctgag agatttggtt ttatagaaag aaaaatcaaa gaacaaggca
     3001 gggaactttc tggtgcaact ttagaggaga tggatgcttt atgggaaaaa gcgaaagaga
     3061 aattcacgcc ggaggaaaaa taagcctcgc tgaatttttg aaacgtttaa aagttatttt
     3121 acctctggtt cagggatgga atcctgacct tttgatagca gttaacccag aaggattgat
     3181 tttgagtggt tttttagcct cttttctgga taaagaagtg agaaatgtgg ctttcaaaga
     3241 agaacccttc cagattacct gggataattt agggaatctg gaaggtaaaa ggacagtttt
     3301 ggtggcagga agattccgag aggaaaaaag cagagaagag atagagaaat gggttaaaga
     3361 ccatggagcg ctttcggtgc tcgtgatggt agcagtaggc gggaaaggag attattctct
     3421 tttcccggaa tggggggaaa atatcctttt gccctgggaa gacaatgagg ttagataaat
     3481 ttctcaaaag ttcttctttg attaaaagaa gaccactggc tcaagcagct tgtaaggggg
     3541 ggagggtttt ggtgaatggt aaagtagcca aaccagggac aggacttaaa ttggaagacg
     3601 aaatcaccat tttcacctct gcttataaaa tagtggtgcg agttaaaagt ctcagcgaaa
     3661 aagaaagagg agaagagttg tacgaggtga taaagaagga gagattagaa gaatgagaat
     3721 actggttgct cctgattctt ttaagggcag tttaaaaagt accgaagttg catcgctggt
     3781 taaaaaaggg ctacagaggg tggataaagg ctggttggta gtggagcgac ctttagctga
     3841 tggaggggaa ggaacggtag aagttctggt gcaagggacg gggggtaaaa taatagaaaa
     3901 agaagtatct ggcccccggg gagagaaagt gaaagcccat atcggggtag tatcttgctc
     3961 ttgtggagag gtggcagttt tagaaatggc tcaagtagcg ggattaacac ttttgccacc
     4021 tgaggaaagg aatcctcgtt ttaccactac ctacggtgtg ggagagttga taaaagaggc
     4081 agtaaacctg ggatttcgtc gcatcgtatt aggagtgggg ggtagtgcta ccaacgatgg
     4141 ggggatggga gcgctgagag ctttgggagt gaggttcttt gaccgggaag ggaaggagct
     4201 aaaaggagtg ggtgatgatt tactgctggt atcttccatt gactctcagc atttgtttga
     4261 aaaaccggaa gaagtggagt tagttatcgc ctgtgatgta aaaaaccccc tttatggaga
     4321 agaaggggca gcttatgtgt atggtccgca aaaaggggcc aatcctgaag aggtggaagc
     4381 actggaccag ggtttacgta attatgcgcg attagtcaga gagcacaccg ggatagaaat
     4441 tgaccattta ccgggtgcag gagcagcagg aggaatagga gcggggttat atgctttttg
     4501 gggagcacaa tttgcctctg gcattcagtt gattatggat attttgcatc tggaagaaga
     4561 aattaaaaaa gcagatttag tgataagcgg agaggggaag atcgataagc aatcttttta
     4621 tggtaaagta gttagtgggg tggaggaaaa gtgtaggaaa tgggggaagc ctcttcttct
     4681 ggtgggagga aaaatcagcg aagaagcttt ttctctgtgg gaagagagag ttttagctct
     4741 tttttccata gctaatggtc ctatcactga agaagaagca tttcaaaagg cgccttttct
     4801 tctggaacac ctggctttta acttaggacg agtgtggaag ggaggagaga tttatgcctg
     4861 aaaagataga gttttccttt ccggagaggg gaattacgat agaagcggag ctaagtgaca
     4921 gttccctggc tgaagaaatt tttcgcattt tgcctgtgga ggcacgagtt agtcggtggg
     4981 gaaaggagat ttattttgct attcctctga aggctcctat cgtttctccc caagaagtgg
     5041 tagaagaagg tgatatagct tattggccgg atggagcctg cctttgtctt ttctttggtc
     5101 ctactccggt aagtaaaaat ggggagatta gaccggccag tgcagtagaa gtggtgggga
     5161 aaatcctttc tccctgggct attctggatg aaataccctc tggtgacata gtaaaaatta
     5221 cccaatttga tagaaaagga gttgataaag gatgagtact atttttgatg ttcatgctcg
     5281 ggaaattctt gattctcgag ggaaccccac agtagaagtc gacgtaattt tggcttctgg
     5341 agcttttggt cgggcatctg ttccttctgg agcttccacc ggtacttttg aagcagtgga
     5401 gttgcgagat ggtgataaaa ggcgctattt gggcaaagga gtacggaaag cggtagaaaa
     5461 tgttaacgaa attatcgctc cggaaatcat tggtttagat gcattagacc aggcggtagt
     5521 ggataagact cttatcgagc tggatggaac gcccaataaa gccaagttag gagccaatgc
     5581 tattctggga gtttctttag cggtagccag agcagcagct gattatagtg ggctttctct
     5641 ttatcgttac tttgggggag ctaatgctcg agaattacct gttcctctta tgaatattct
     5701 caatgggggg aaacatgctg atagcggagt ggatatccag gaatttatga ttgttccctg
     5761 tggagctcgt tcttttggtg agagcttaag aatgggggcg gaagtctttc ataacttagc
     5821 cagggtttta aaacggagtg gttactcaac aggtgtagga gatgaaggag gttttgctcc
     5881 caatctccat tcttctgagg cagctatcga ggtaattctg gaagctatac agttggctgg
     5941 ttatgaaccg ggtaaggatg tttatctggc gttagatgtt gctgcttctg agctttataa
     6001 agatggaata tatgtttttg agagggaagg ggtgcgccgt aacgtagagg aaatgattga
     6061 tttttaccgc cgtttggtag aaaattatcc tgtcatttct atcgaagatg gtttagccga
     6121 ggaagactgg gaaggatgga aaaagctcac ttttgccctg ggtgataagg tgcagttagt
     6181 aggtgatgac ctgtttgtaa ccaacaaaga aaggttactc cgggggattc gggagaaatg
     6241 ttgtaattcc attcttatta agttaaatca aattggaact ctcaccgaga ctttagaaac
     6301 tattgaaata gccaagagag ctggatatac ctcggttatc tctcaccgct caggagaaac
     6361 tgaggatagc actattgccg atcttgcggt gggatgcaac gctggtcaaa taaaaaccgg
     6421 ttctctctgt cgttcagaga gaacagctaa gtataatcag ctattaagaa tcgaggaaga
     6481 gctggataat gccgctattt tccgaggaaa agaggttttc aaagtttccg gttactgaaa
     6541 agggatggga gaagtttctg ttcatcgctt tcttggcgct ttctctgttt tcttttaact
     6601 tgcgcctgag taataaattg gtagattatt ttgaccttgc tcgggagatg aaagagttag
     6661 aaaagaggga aagcgcacta tctgaggaag tggagaggtt agaaaaagag agagaatact
     6721 tagacgaaga ttggtatgta gaaaaattgg ctcgagagcg attgcaattg gtaaaaccag
     6781 gagaaattgt agtaaaagtg attaatcccg agtcaccgga gcattgacaa tctttgttct
     6841 tctacctata atcgttttat acaaatgtgg tcggggcgtg gcgcagcctg gcaagcgcac
     6901 tcgcttgggg tgcgagtggt cggccgttca aatcggctcg ccccgaccaa aaccctgacg
     6961 caattttctt agtataagga ggataagaat atgccctcaa atgtcaaaga tctttttagc
     7021 ctgcaaggag aagtagctat tgttaccggt ggagcacaag gtttaggaga acaaatgtcc
     7081 atcgctttag gggaagcagg agctcaggta gtagtggcag atttgaatct ggaaaaagct
     7141 cagaaagttg cctctcatat tcagtctttg ggagtggatt ctctggctct ctgcgttgat
     7201 gttaccaaaa aaggagaagt aggggaaatg gttgagaaaa ctctggagcg tttcggcaaa
     7261 attgatattc ttgtaacttc tgctggggtg ggacaatgga tgaaggcaga agaaatgcca
     7321 gaggaggatt ggaaaaaagt tctggacgtc aacttaaatg gagtattttt gtgttgccag
     7381 gcagtgggaa gagagatgat taaaagaaaa aaagggagta ttataaatat ttcttctatg
     7441 tcgggattag tagtcaatac tcctcaggct caatctcatt ataactcctc aaaagcggga
     7501 gtaattatgc taactaaatc tctggcctcc gagtgggctc cttacaatat tcgggttaac
     7561 accattgctc ccggttacat ggaaacgcaa ttagtgggtg accttttgaa agaatatcct
     7621 gattatgctg acctgtggaa aaagcttact ccttttggtc gtctgggtaa cccggaagaa
     7681 attaaaggtc cctgtgtttt tctggcttct cctgcttctt cttatgttac gggaagcgtt
     7741 ctggttatgg atggaggcta tacgatatgg tgagggaggt gatagtaagg ttttaagatg
     7801 ggagaagttt atttagtttt tgggattgtg aaagaaccaa aaaagaaagg agacgatttt
     7861 ttgtgagaag acgtttatta actgtagttt ttgttttgtg ttttctgctg gttcctacca
     7921 tggtttttgc tcaggtagct aatcctgatt ggtggaaaaa agcagccgag ccttataaag
     7981 gagtggtgat tcacggtgtg tcggaaagca ctcctccatc taaagctata gcagagatag
     8041 ctgttcccgc gtttgaagag ttaaccggaa ttaaagtaga gcttgaaacc acctcctggg
     8101 atgagatgta taataaagaa attcaggaca tgcaggcggg agccgggata tacgattttg
     8161 tctacctgga gcaggatgca gtttacagct acatagagca agattggctg accgatttaa
     8221 ctccttttgt cgataatgct gatttaactg aacctgattt tgatcttcca gattttacca
     8281 gttttattga ttactttaaa ggcaaagacg gagaacttta tggtatcccc tttgaagctt
     8341 ttctgaagac ctatatttac cgcacggact tgttcaatga ttctgatatt caagctgctt
     8401 ttaaagagaa gtatggatat gacttggctg ttcctactac ctgggagcaa tacgaggata
     8461 ttgctaaatt cttcactgaa tacggaaaag aaaaaggtat agaactttat ggacacgtag
     8521 cgcaagcaaa gacccatcct tgtttggctt atgaactttg tgaaactatt tggcctccct
     8581 ggggtgtata caattgggga ataaatctag aaaatatgag aaccagcgta gaaaatggag
     8641 gcactctaaa cagcgatacc gcgaaagaag ctcttggctg gtatgttgac atgttacaat
     8701 atgctcctcc aggagtcaga acttatacct gggatgaggc agcagctgtg tttggaaccg
     8761 ggataattgc ccaagggttc ctttatatgg aaaacttagg ttggttagct catgaccccg
     8821 atcggtcggt ggtgatcggc aaagttgcaa ttgacattcc acctgctaaa ccgggtgtta
     8881 tggaagcagc tgaagctggc gaaggataca tcggttacta tgatggtgga gcttggggaa
     8941 ttccttactg ctccaaaaac aaagaagctg cttggctttt catccagtgg tgtactcgta
     9001 aagaatggca gccagagttt gctgaattag ctactactgt ggtaaggaaa tctgcctttg
     9061 aagatccaat aatcgatgaa ttaaatgtag caatgggtgg ctactacact gtcatgcagg
     9121 aaaagggtca tctttttgcc ggatgtcctc ctctacccat gcaaacacca ctcaatgagc
     9181 tttatctcca gtggatttcc cgggcagtgg ccggagaagt ttctcctgaa gaatgcctgg
     9241 ataacttagc taaagaagct gacgaattaa tggaagagct aggctattag gaggtagaga
     9301 gtttgcgagt aaaaaagact caagtgtggg ggttaatatc ccccacactt attttaatga
     9361 ttcttatcgg ggtaatcccg gttatatacg tcatttattt gagtgttttt aaatacagtg
     9421 tgttttccaa gacgggaatg gtttatgtcg gtttcgataa tttccgcaaa ctggtatttg
     9481 atgcctcttt tttaaagtct ttaagcttgg gtcttacctt tgtggctatt tgctgtctcg
     9541 ttgaaatacc tttaggactt ttgattgcca atgtcctcac ttatgagttt aaaggtaaag
     9601 gtgtttttcg gacgattatg gcattgcctc tggctatggc tcccgttagc attgggagta
     9661 tgtgggtatt actcactaat cctgatgtcg gtccacttcc ctattttttg ggaaaaatcg
     9721 gggttgacta taatatcggg gtaaatgcta ctcaggcgtt tgccaccact gtttttatgg
     9781 atgtctggca ttggactcca ttcgtaattt taacttttat ggctggattg gcttctttac
     9841 ccaaagaccc gtatgaggct tctttggtag atggggccaa tcgctggcaa acttttcgtt
     9901 atattactct gcctctcctt cggccagtga ttttaactac cttgtttatc cggattatgg
     9961 atactttccg tgtctttgat gaagtatgga tgttaaccag tggcggccca ggaacagcta
    10021 ctcgttttgt cagtatccat ttggtgagag aagttttgcg atctatgaat tatggataca
    10081 gttcggctat gtctcttttt ctgctttatt taaccatagt tatctgctgg ctattgttga
    10141 cttttatttc catctcgcag gaggaagtct aaatgaaaat tagcaaaaaa ggactccggc
    10201 tcttttttat ctatgttttg tgtattttaa tagcgatagc tactctgttt cctatctact
    10261 ggacatttat aatctctgct caatctaaac tggaaatttt ctctgggcca aattttttcc
    10321 cttctacttt tcatagtgaa aattataccc gtcctttctt gcaagatgta tacggacgct
    10381 atctggctaa ttctttgatt gtggccaccg gtaatactct tttggtaata gttttagcag
    10441 tcatgtctac ttatgctttt tcccgttttc agatccccgg gtcaagtaat atctttttct
    10501 ggactattac caaccgtatg gctccagcgg cagtttttat tgttcccttc tttttgatat
    10561 ttagtcgcct ggggttgaga gatacgagaa ttggtttaat tatcctttac tgtattttta
    10621 acctcccctt tgccatctgg cttttgaaag ggatgatgga ttctataccg aaagaactgg
    10681 atgaggcagc catgatcgat ggatgttcta tttggggagt gttggtgaga gtaattattc
    10741 ccttggccaa accaggaatt atggttactg ctattttgac ctggattttt gcctggaatg
    10801 aatatttgtt tgcctcgatt ttgactaatg ttaaagctcg gactataact actggtttaa
    10861 ttgagtttgt taccgtgatt gggactaact ggggtgaaat ggcggcggtg tctatggtct
    10921 gtttaattcc ggctgtggta tttattggta tagcccagca gcatattatc actggcctta
    10981 cttttggagc ggtgaaagat taggaggata ttatgcggat tttacctatg aagactaact
    11041 tatttgatcg gattttcatt ggagcagtgg ttatgtttgg tttacatctt ttttgggtac
    11101 gcttcgtcga gcaattcatc cccctttatg tagccactat aggcagtctc atttttctgg
    11161 tgactttaat tataactggg taaaaaaaga gtaggggagg tgacatccgc ctccctattg
    11221 ctactttatg tactgtacgg ttcgcttata gtttactacc cgaaatgtat gtttatctct
    11281 accgctccag tttctatttc ttaataaggt acaaaaaaat agggagggaa actgaggttt
    11341 ccctccctgg aagctaataa atgttgtggt agtacgtttc tatccttaag taattttcaa
    11401 aagcttcaga caaaatatct ccgctttgag ataagctaat agctacgtgg tgttcaaaac
    11461 cattgatgca aatgtatttc agtaaatctt gaaggtcttc gatttttgct acccctactc
    11521 ctccaaaagt gttaacctta tcgggagtga tttctccttc tgctatataa gatattaagt
    11581 tgccctcttc atcattggtg gccaagcgag ccagagttag ctctccatta ggtatagttc
    11641 cgtaacaagt cccgtaagct ttttctttgg aaaccgaaga ggagatgatg tcgccatatc
    11701 ccattctggc tccctgcaga agcgatttgg ggaagttgct gcagtggaaa agaatggctt
    11761 tgtcggggtc gtcgccaaaa ttattattcc agtccacgag agcagatggt ttttgagaag
    11821 cgagctgcag agcatacatg ctcaatcctc ctaccaggtc tacttcgcag gctgcgggta
    11881 ttaatttttc gctgaacatg ctcattactg tacagggcat gatgccataa ttttcttcga
    11941 tagaattcca gcattgaaaa gaaaagaggg atacaccggt agcttcaatc cattcttcta
    12001 ataccacgag aagtttagcc attttgacca acgcttcttc gggtattcca gcagtatcaa
    12061 tataatcttt tatttcctgc aatttcccct ttactttcag attattgtta tctaatctat
    12121 tcatgttgcc gattatttcc gagaggtcta tggtttctac cgatattcca tagctctcca
    12181 gaatcttttc tgagaagcga acggtattaa aggctgcagg tcgtgctcca acagctccag
    12241 cttttaggtt tttaaatccc tttactactc gacatactcc cgcaaaccac tctaaatctt
    12301 ctccgaagat tgaagaatca gggtcttcag tgtgataaga agttagagaa aaggggatct
    12361 ggaattgctt caggttattg catatagaaa gcttaccaca gaagctatcc ctccgatttt
    12421 gaacatccag agtattgatt tgatcaggaa aggcatgtac taaaaccggg acgttcaacc
    12481 ccgagagacg aatagtttga gctgctgctc gctcatcgcc gaagttgggc agagtgatga
    12541 taattccctc tatgcgatgg cggttttcat cgaaaagacg ggcgcatttc tgagcatctt
    12601 cccaggtttc aatagtacca aatttggtgt cttcggtgct gggagccact acttcaaaac
    12661 cttttttttc taaaacttcc aggattttac tccttccttc tttggctaac tcatcaggaa
    12721 agaaacctcg gttacctaca attaaaccaa aagtttgtgc catacttatt tctcctttca
    12781 tgttaaaata ttaaaaaagt ttaattttat ttaaataccg agttaacatt cttatcttac
    12841 catattttca gaataaaaaa ttaagaaaaa gggaggtaag ataaatgaaa aaggtgctgt
    12901 taagtagctt ggtgttatgt tttatcctct ctctttccgg cttgagctgg ggggtagaga
    12961 aagttgatgt gcagggttcc actactattt tacctattat gcagataata gccgaggaat
    13021 ttatggctca aaacgaagac attgaagtta cagtaggtgg aggaggctca ggagtgggca
    13081 tttctgctct tttggatgga gtgaccaatg tggctatggc ttcccgcgac atgaaagaag
    13141 aagagtggca aaaggctaaa gagaagggtt tagatatccg ggaaatagaa atagctaaag
    13201 atgctctttc agtcatagtt aattatctta atcctatttc ggaaatcgat gtcgacactt
    13261 tgcaaaaaat ctatactgga ggaataagca actggcaaga attaggggtt gctttagatg
    13321 gggaaatagt gattatttct cgcgatacta attctggaac tttcgaagtc tttaacgaac
    13381 acgtcttaag aggagaagaa ctcgctgata aagctttacg tcttccctct aaccaggcag
    13441 tgttgaatga agtggtcagt aaccccaatg ctatgggata tgtgggattg ggctatgcac
    13501 agcagggagg agataaaatc aaagctctta ctgtgaacgg agttgaagca agtcccgaaa
    13561 aagcactttc tggagaatat ccactgtctc gaggattata tctctatgtt aatggcgaac
    13621 cccagggagc ggtcaaaaag ctgattgatt ttgtcctggg tcctgagggg caaaccatag
    13681 tggaagaaga aggctttatt cctctttctc taaatgaaga ttaaaaccat ggggggagaa
    13741 tatcccccca tatagtttac tagatagcac ctcggggaaa taacctcttt atcccccgga
    13801 aaggtgccag aactttgtaa tggtggggtg aaaggataat ttgggtttag atatagtttt
    13861 gatagaagac gagaaagacg tagttgattt tgtcacttat tttcttcaga aagaaggtta
    13921 tcaggttttt ccctttttgc ggggtgaaga tttcctggaa aagatggata ctcttcttcc
    13981 cgatgtcatt attttagacc ttatgcttcc aggtattgat ggtttagagg ttttccgcta
    14041 tattcgagag tatcctcgat ttagaaacgt tccaattatt atgcttacag ctaagagcga
    14101 ccctctggat gtggtgttag gattggaaat aggagcagat gattatgtta ctaaaccttt
    14161 tcatcctcga gaacttttag cccgaatcaa agcagtggtg aggagaactt cctctccttc
    14221 tttttctgaa aaagaagttt tgaagatagg agatttctgg ttagattcgg atcatctgat
    14281 ggtaaaagaa aatggtaaag aaatttacct ttcctggaga gaatttcgct tatttgaact
    14341 tcttattaaa aatcggggaa aagttatcag tcgccagcaa attttggata gcatctgggg
    14401 agaagatagt tttattactg atagagtagt agatgtttat atcgctaatt tgcggaaaaa
    14461 actgggtagt agtgggaata aaattgtgac ggtacgagga gtgggttata aatttgaatc
    14521 tacttaatat ttttctgttt ataattctta tagttttttt tctattggga atttactttt
    14581 atcgaaagtc aattagcaat cagcagtcca aaacccatta tatgcttcta gaacgagaaa
    14641 atgctgaaca ggttctatcc ctgaaagaaa atattctaca agctatggat tctctatctc
    14701 tggcgatggc tttttatgat ggtcaaaagg aaaaagtgat gtttaactct tcttttggta
    14761 aattcattgg tgggaaagaa aaaggagagg cgtttctctg ggaaataccc cttctgggaa
    14821 catggttgag aaaagaagag agtttacaag taatagaggt aggaagtttg aaacttcacc
    14881 tccggatttg gggaggagaa aatgataagg tagtttttct tgaagatata ggagaaaaag
    14941 aaagaaaatc ccgggaaatg aactaccttt ctaccgccct ctggcatgag ttacgcactc
    15001 ctcttactgc tcttgaaggt aatgttgtta tcttagaaga agaagtaaag gaccaatttt
    15061 tgcttgatat ctcctataaa atggagcagc aaataaatag gatagagact actttggata
    15121 accttcgcca gttgacctct atttttcaag aagaaaacca gttttctact gctggagaac
    15181 taaagcgaac cttagaagaa gtgataaata attttgaaga tgatattcgg agattagatc
    15241 tcagtttgag cgtagatact tctcttttgg ataaagtaaa ggccatattt cctttttctt
    15301 cagctgattt tttgatcctt ttttctaacc tgttttccaa tgctctcaag tttaattatc
    15361 ctaaagggag agcagaaatt aaattgaata gagaagaaga gggtgtattt ctggaactca
    15421 gcaatactac ctctaacact tctcgagaat tcataaaatg gctttttgaa ccttcttcta
    15481 ctttttctcc tcgaggagct gatggaagag gagtggggtt atatttagct aaagaggctg
    15541 tggcgcggat gggcgggaga atcagtgctc attatgggga aggagattcg gtaatttttc
    15601 gggttttgtt gtgtgttgag gataaaaacg aagaaaactg attgagccga atctacaatt
    15661 tttaaactta tttacattcc cgattactga cttcgggaat atcggaaaca atgaaatctc
    15721 aaaaaaaaga ctctcctctc ctagaaaaaa agagaggaga gataggcgtt tgataaataa
    15781 tctggaaata atgcggagag taacttacgt tacactttac cctctgcgca agaggcaatt
    15841 atacattccc ttatcgcttc ttttactttg ccttctatcc cctgtaaaat tttacccacc
    15901 atgagctctt cgggatggtt cgctaagtat tcttggaaag cacttaagta ttgcactcgc
    15961 aaggcggtgc taaagtttat ctttctcact cccagttgcg ctgcctttcg tagttgctct
    16021 cgaggtgtac cgcttcctcc atgaatgacg agaggtaaac tggttttcgc tctgatttct
    16081 ttaatgcgtt caaattggag ctggggtgtt cttcctttgt ataatccgtg acgagttcct
    16141 actgccacag ccagcatatc tactggtgct ttaccagtaa actcctgcgc ttcttgaggg
    16201 gaagtaaaga aatcctcttc tttgcttttc tcttcccagc tttcatctcc ttctaatcct
    16261 cctactcttc ctaattctcc ttctactagt acctccactg gatgggcaat ttctattact
    16321 ttattggtta ggttgatatt ttcttctaaa ggtagacgag aaccgtcgat cattaccgat
    16381 gaaaaaccat tttgtatggc taaaagaata tcttctaggt gatgagcatg gtctaagtgg
    16441 agagaaaagg gtatagaaaa tttctgagat agaaaaagta acgttccata aaacatcctt
    16501 atatccaggg cttcatattc caccggagct acctggataa tcaggggagc acgcatttct
    16561 tctccttctt ccaaaaccca caaaataaac tcaggtatag gaacgttgaa agcaggaaga
    16621 gcaaagttct tctcttcagc taattttaga atagtttgag tagagactaa aggcataaat
    16681 aaagctcctt tctttttggc tgttctgaaa ttataacaga ttttttctct tttcctggaa
    16741 atattttttc aatgttcaga agagctcatc atttcttgtg atggggacag aaactattta
    16801 ctatccttat cgtaaggggg tattttggag gtataaaaca caaaaggtgt ctaccaggcg
    16861 gtagacacct ttttatagca agcagaagtt aaagattttt tattttttct tctctttctt
    16921 gtaatagttt ctcgatggct tctatatctt cagcggctaa ctcccaatct ccagcgggaa
    16981 atgtttcttc aatctgagaa ggacgtcggg ttcccactat agcagcagta acctccggac
    17041 gtcgtaatac ccaggcaatg gccagttgag ccagggttct tccgtgtttt tcagcgatgg
    17101 gttttaattt ctctactaat tccaggttga tactgagcaa gggttcatta aattgggggt
    17161 cactccggcg atggtcatca ggaggaagat ttttcacccg ctccggggta aatttgccag
    17221 ttagcaatcc cttttgcatg gggctataca ctattactcc catatttttt tctgcacaat
    17281 aaggcaaaat ctctttttct atatctcgca taatcatgct gtaggggggt tgcagagaag
    17341 cgatgggatg gattttttca attcttttca tttgttccac gttaaaatta gaaactccag
    17401 cgtaacggat tttcccttct tttactaaat cggccatggt gctccaggcc tcttcaatat
    17461 cggggtcggg atctggccag tggatttgat agaggtctat cacttccact tccagtcgtc
    17521 tgaggctgtc ttctacttct ttgcgaatgc tgtctctttt taagcaccca aaaacttctc
    17581 ccttctcgtt ccataccaat ccgcatttag tagcaataat gggtttttcg ctcatacctc
    17641 ttaaggcttt agctataatt tcttctgaat gtcctaatc
//
LOCUS       DAOPSS010000021        17658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2697_length_17658_cov_8.220076, whole genome shotgun sequence.
ACCESSION   DAOPSS010000021 DAOPSS010000000
VERSION     DAOPSS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 17658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17658
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_17658_cov_8.220076"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PKN31_02470"
     CDS             complement(<1..485)
                     /locus_tag="PKN31_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98827.1"
                     /translation="MLLSELNPGQRFLLFLGKNWVVFFLLFLCVLFSFTGTGFLSPKT
                     LNNILITSSPALLLAAGETFVIITGGIDLSVGFVRGLISVISAIIMRDLYAAGYSPYV
                     AILWGIVVGIGIGLLPGLVNGILVAKFRVPPFIATLGIYGVANGFALRLSQGFPITFL
                     PP"
     gene            complement(534..1523)
                     /locus_tag="PKN31_02475"
     CDS             complement(534..1523)
                     /locus_tag="PKN31_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA98828.1"
                     /translation="MRKNFLTIFIVSLLVLVLVGGVLAQEKKYKIAYISGILDPFQVL
                     IGEGIKDKAAELGMDAFIAEHPKAWGPEVQVPILEAVAARGDIDIIAIVPTSQEALIA
                     PLKRIYDSGIDIITVDTYIGDGDYSKPSDWSFPLAYIGTDNFQGGVEVAHHLAEMIDY
                     KGKVFVETTNPDTSSVVERVEGFKKGISEYPDIELVGVEYCLDVQEKAQQQVAAALQA
                     HPDIVGIFGTNLYSAQGAYQAVANAGLAGVVKIAAWDATEDLIKALKEGQVDLVLAQK
                     PYEIGTLAAEWAYKYLVEGQEVPKRIVPGFFFFTRDNVDDPEAQQYIYRMPQD"
     gene            1795..2967
                     /locus_tag="PKN31_02480"
     CDS             1795..2967
                     /locus_tag="PKN31_02480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOA98829.1"
                     /translation="MNNFEFLSPTKIVFGKGTENRVGQEIKKCQAEKVLLHYGGGSIK
                     RFGLYDRVVASLKESDIDFIELGGVKPNPRVSLVREGIKLCRERGVDFILAVGGGSVI
                     DSAKAIGIGVPYDGDVWDFYTGKAEPQETIPVGVVLTIPAAGSEASPGSVLTNEDGWY
                     KRAVNAEIMRPRFAILNPELTFTLPPYQTVCGASDIMAHVMERYFTPTRHVDFTDRLC
                     ESTLKTVIKNVPVVLDQPDNYDARAEIMWAGTIAHNDLLSTGRVGDWASHDIEHEISG
                     IYDVAHGAGLAVVFPAWMKYVYRENRDKFVQFAVRVWNVDMDFEDPERTALAGIDQLK
                     RFYKNVGLPTSLKDLGISDDRLEEMADKCTAGGTKTVGNFKRLGKQDVLNILKMAL"
     gene            3874..5430
                     /gene="pckA"
                     /locus_tag="PKN31_02485"
     CDS             3874..5430
                     /gene="pckA"
                     /locus_tag="PKN31_02485"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOA98830.1"
                     /translation="MQSLFYPLEKAGIHNPRYVYYNAVVSQLLEEAIKREEGIFTSTG
                     AFNVLTGKYTGRSPEDRYIVDDEITHNSIDWGKINVPIEPEKFNRLHGKLCAYLQGRD
                     IFVYDGFVGVDPQCRLAVRFINEYAYQTVFVENMFLEATREELTNFKPEFTVICAPNF
                     KADPEEDGIRSEAFVIINFREKMVIIGGTSYGGEIKKSIFTVMNFLLPQQGVLPMHCS
                     ANVGERGDDVAIFFGLSGTGKTSLSADPRRRLIGDDEHGWSEKGVFNFERGCYAKCIN
                     LSREKEPQIWEALRYGAIMENVVVDPVTREADYNDARYTENTRASYPIEYIPNALIPG
                     MAGHPEVVILLTADAFGVIPPVAKLSREKAVEFFLLGYTSKLAGTERGIVEPQATFSP
                     CFGAPFMPLHPQIYAKMLGEKISQHGSRTYLVNTGWMGGPYGVGKRIDITYTRAIVHS
                     LIDGSIENYPFREDPIFGFLVPEEIKDVPRELLHPRSTWRNGSEFDEQARKLQSDFAK
                     TINRFKFSAV"
     gene            complement(5597..6025)
                     /locus_tag="PKN31_02490"
     CDS             complement(5597..6025)
                     /locus_tag="PKN31_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOA98831.1"
                     /translation="MARQLSIFAENKPGRIEKITRLLSQEGINIRAITISSANGFGVI
                     KILASEPQKAFELLRNHNFPVYLQEVIAVVMNDEPGGLHRVARALAENQINIEDAYGF
                     VIKSGEKAVLVIQVENQPQAHNILERNGFQLLTDEEIYQL"
     gene            complement(6040..7338)
                     /locus_tag="PKN31_02495"
     CDS             complement(6040..7338)
                     /locus_tag="PKN31_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOA98832.1"
                     /translation="MYWEKQLETIPREELSLLQAERLKKTIANAQRTSFYRRLFSHNK
                     IDPEKIKSPEDLKFIPPTTKVDLREGFPFGFLAVPLEEAVRLHSSSGTTGTPTVIYYT
                     ASDLDRWTNQVARSMYAVGMRKNDVFQNMMSYGLFTGGLGMHYGAERLGALTIPIGAG
                     NSKRQIWFLKFFKSTAIHIIPSYALLLAQLIKAEDLDPRQDFNIRIMLLGAEPHSEET
                     RRRIEEIYGAKAYNSYGLSEVNGPGVAFECVFQEGMHLWEDYYILEILNPQTLSPTPP
                     GEVGEIVLTTIYRDATPLIRYRTGDLASLLEEPCPCGRTHRRISRIQGRTDDMFIFRG
                     VNIFPMQIEKVLMSNPEVGNNYRIILEREDYRDKMIVEVEIKPELFLGDLQKLARLRE
                     RIQSELHSEILVTPEVILVEPGSIPAPEGKVQRVIDHRQL"
     gene            7573..8298
                     /locus_tag="PKN31_02500"
     CDS             7573..8298
                     /locus_tag="PKN31_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOA98833.1"
                     /translation="MSLDLNVFISFLAGLLSFLSPCVLAIAPAYVSYISGVESTEKDR
                     TKTITHTLFFVTGFTGIFVLMGLGASFLGSIIAPYRVWFNRLAGIIIIIFGLQILGVL
                     KIRWLYADKHFRPREINLSQVRSLLLGISFGLGWTPCVGPILGSILLYASTMGKIGQG
                     GILLFFYALGLALPFIFLGIGWGHLTTFFRGLQKRGRWVEITSGILLVFLGVVIFTGE
                     MNALFSLLGVDASFNPEFLLFRE"
     gene            8310..9128
                     /locus_tag="PKN31_02505"
     CDS             8310..9128
                     /locus_tag="PKN31_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOA98834.1"
                     /translation="MPIIGKDEQKVKAFGDVSKRLNLSNRVYRGMQAIPVDKIVGSVG
                     RAGDLLPGFRLKNPDTRYYRIRRAMERGEVLPPIIVYQVGDEYYVLDGNHRVAVAKEL
                     GVKYIDAEVIEFFPSELRVSRALRKKRAEFENLTGLKGIDASEPRHYDSFLSYIKDYA
                     KSMETFLGREVPLKEASLNWFLSVYTPAVQAIVERGLEEAFPDKSLADIFAYVLDYKW
                     YKSQKEGYDIGFEQALEGFIQEIVGKQESEENKRTFMQKVGGLLEEVLPWLRKE"
     gene            9110..>9727
                     /locus_tag="PKN31_02510"
     CDS             9110..>9727
                     /locus_tag="PKN31_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOA98835.1"
                     /translation="MAEKGINWHELSPEEVIKILNSNEKKGLSEEEVKQRLEKYGRNT
                     LPQPRKKTIFTMFLEQFKDFLVLILVGAALVSLLLGEITDSLVIIAILILNAVLGVVQ
                     EWRASQALEALKKMTVPECEVIREGKPLKVNSEEIVPGDIVILREGDFVPADLRLIEI
                     HALKIDESSLTGESVPVEKDISPLPLDIPITDRLNMAYSGTLVTYG"
     assembly_gap    9728..9827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9828..11360
                     /locus_tag="PKN31_02515"
     CDS             <9828..11360
                     /locus_tag="PKN31_02515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOA98836.1"
                     /translation="QIAVLCNDAIVEEGEEGEEVRLGDPTELALLDYVWEQNVDFNSW
                     RKLYPRQEEVPFDSKRKMMSTLHNIEGEMALLVKGAPDIVITRCSFYEKEGEIIPLSP
                     EKEQEIKKMMEEMAEKALRVLAFAWRKMEEGIVPSPEEEKDLIFVGLIGMIDPPRPEA
                     KLALEEARQAGITTVMVTGDNPLTARAIAEELGMFSPQDLVVTGQELANYSPEELKAN
                     IKKIKVFARVWPEQKLKIVEALQAHEEVVAMTGDGVNDAPALKKADIGVAMGISGTEV
                     AKESADMILTDDNFATIVQAIKEGRVIFDNIRKFVVYLLSCNLGEIFTIFIPILVGWH
                     SPLVPVQILLINLVTDGLPALALGMDSPEPDVMMRKPRHPREGIVNPHYMGIVLFNAF
                     FITLAVVASFGIGLALWGREISRTIAFATLGFDELWRAYSFRSEKRNFWQIDPRGNLY
                     LVGACLLSFLVIILVVLLPPLQGVFQTATLSLNQWILVVLFSILPVSAYEVRKLFHKK
                     EE"
     gene            11365..11847
                     /locus_tag="PKN31_02520"
     CDS             11365..11847
                     /locus_tag="PKN31_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOA98837.1"
                     /translation="MEEKIVKIENPQGYNLILGQAHFIKTVEDLYEALITSSPHLKFG
                     LAFCESSGPCLVRYAGNDEELTELAKNNAWNISAGHTFIIFLKDGYPINVLNTVKMVP
                     EVCHIYCATANPVEVIIKETEQGRGVMGVIDGFKSKGIEGEKEIADRHKLLRQIGYKL
                     "
     gene            11858..>12702
                     /locus_tag="PKN31_02525"
     CDS             11858..>12702
                     /locus_tag="PKN31_02525"
                     /inference="COORDINATES: protein motif:HMM:NF017380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOA98838.1"
                     /translation="MKRATADLPLHGGKCPPWLFERMKTLGGLIMEAIVEFFGEEEIL
                     CRISDPVFFQSLGCLLGFDWHSSGLTTTLCGALKEGVRGKEKDLRLFICGGKGATSRK
                     TPEEICLHAQKHALPFADQMVYRSRMSAKVDNSALQDGYNLYHHTFFFTFSGKWAVVQ
                     QGMNPDTKYARRYHWWGEGVKDLVCEPHQGIWGEKKEREVLNLVARESTDCREGIVKL
                     AQEKPEKLKKEMALVPHLSLPSRHFIKEEDFNLGRIDKTLHMIYEEKPQNFENLLALR
                     GVGPKT"
     assembly_gap    12703..12802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12803..13186
                     /locus_tag="PKN31_02530"
     CDS             <12803..13186
                     /locus_tag="PKN31_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOA98839.1"
                     /translation="SANNSQPWEFIVVTDPDKRREIANATDYGKFIAQSPVCVAVFCR
                     DTHFYLEDGCAATENILLAARDLGLGSCWVAGDKKHYSPQIAEILNVPDDYRLVSLIA
                     LGYPAVEPAMPTKRSLEEVLHWEKF"
     gene            13198..13677
                     /locus_tag="PKN31_02535"
     CDS             13198..13677
                     /locus_tag="PKN31_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA98840.1"
                     /translation="MKVGILSDSHDNLASLRQALDILEKEKVDLILHAGDYVAPFSVE
                     LLTRFSIPWEGVLGNNDGEILGIFQKSGGKVNAPFWEGEKNSFRIWISHFYLPAQLAW
                     ESRKYNLVVYGHTHEAVIKEENGVFLINPGEVCGLLSGRATLALCDLKEGVAKLIDI"
     gene            13709..14371
                     /gene="phoU"
                     /locus_tag="PKN31_02540"
     CDS             13709..14371
                     /gene="phoU"
                     /locus_tag="PKN31_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOA98841.1"
                     /translation="MEIIRERFTQQLKELEEDLLNMTHTAAKMLQMSIESLKERNIEK
                     AEEAIALDDVVDDFNFKIEEKCLSLIALQQPVAKDLRVIAAILKVITDVERIGDYSVD
                     IAKFSIRLAEKPLFKPLIDVPKMADLVRKMLEEAITALVQRDLTIVQKVVEADDQVDA
                     LYRSVHEEVVEHIEKDPSVTRQAIWILMIARYLERVGDHITNIAERVYYMETGELIEL
                     HQ"
     gene            14381..14968
                     /locus_tag="PKN31_02545"
     CDS             14381..14968
                     /locus_tag="PKN31_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOA98842.1"
                     /translation="MEEKKIWYFPQSGPDNTLATLDIALERAKELGIKHMVVASRSGD
                     TALSLEKKAREVDYSGNLVMVTYHAGFCGEDSISFTPSRREELEKKGVKIVMSSHALS
                     GVGRSFRQKFGGVSIPEIIAESYRRISQGFKVAVEIAIMAADGGVIPTTDDVISMGGR
                     GKGVDTAILLRAAHQNSFFDLKIKEILCLPREEKT"
     gene            14984..15433
                     /locus_tag="PKN31_02550"
     CDS             14984..15433
                     /locus_tag="PKN31_02550"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOA98843.1"
                     /translation="MINTFKATGEWKGGLRIDTQVRNFTLSFDEPPSLGGEDSACNPV
                     EGVLASLAGCLGIVSRVVAQEKNLPIEKIEIEVEGDLDPRGFMGQYDQVRPGLLAIRY
                     QVKVNGDLSDEELKEFEEEVDRRCPVSDTLKNGTKVEGRVTLEQSTA"
     gene            16032..17069
                     /locus_tag="PKN31_02555"
     CDS             16032..17069
                     /locus_tag="PKN31_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOA98844.1"
                     /translation="MMKAAILEDIDRLVVREVEDYEVGKGEVLIRVKACAICGSDIRI
                     MHHGNPRVKFPHIIGHEIAGVVVEVGEGVEKFEIGDRVAVGADVPCGKCFWCQNGMGT
                     NCAINYAIGYQFPGGFAQYILLNSLSVEQGSVHKIPENLSFDEASLAEPLACAINGLE
                     MANLEVGDTLCIIGAGPIGCMMIEMGRHMGATNIIVIQRSQLRLDLARKFNADHYFLS
                     DRHVEEKVKEVTDGEGADVVMVTCASPEAQEQALLLARHRARINFFGGLPSGARNISI
                     SSNLIHYQECMVLGSHGSLPRHHQKALRVMEKGMVNPAEYITHRFPLEEIQEAFRVAE
                     SHEGLKVVVNP"
     gene            17066..>17658
                     /locus_tag="PKN31_02560"
     CDS             17066..>17658
                     /locus_tag="PKN31_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOA98845.1"
                     /translation="MKALVLEEKGVLSLREVKTPVPREGEVLIKVRAAGICGSDLPRI
                     FGGQAYFYPLIPGHEFSGEVAKVAEEEDEEWLGERVTVYPLLPCRDCEYCEVGQYELC
                     NNYGYLGSRQNGGFAEFVVAPVENLLPLPTSVSWEEGALSEPGAVTLHALRRAHPEVG
                     DRVAVFGLGPIGLLCGMWAKLGGARMLTGYDVDEQKFP"
BASE COUNT         5049 a         3227 c         4147 g         5035 t
ORIGIN      
        1 gggggaagaa aggtaatggg aaaaccctga gaaaggcgca gagcgaaacc attggctacc
       61 ccataaatcc ctaacgtcgc aataaaagga ggaactcgga acttggctac caggataccg
      121 tttaccagcc cgggtaagag acctattcct attcctacca ctattcccca gagaatggct
      181 acataagggg aataacctgc agcgtaaagg tctcgcatga taatagccga aattacggaa
      241 attaatcctc gcacaaatcc cacagaaaga tcgataccac ctgttatgat gacaaaagtt
      301 tctcccgccg ctaaaagaag agcgggtgag gaggtgatga gaatgttatt taaagtctta
      361 ggagataaaa aacctgttcc ggtaaaacta aaaagaacgc acagaaaaag gaggaagaaa
      421 actacccaat ttttacctaa aaaaagaaga aatcgttgtc caggatttag ttcagaaagt
      481 aacatttttc accctttttg aaaggaggga gggtagacct ccctccttta gcttcagtcc
      541 tgaggcattc gataaatata ttgttgagct tctggatcat ctacattatc tcgggtgaag
      601 aagaagaaac caggaacaat tctcttgggg acttcttgac cttccaccag atacttataa
      661 gcccattctg cggctaaagt tccaatttca tagggttttt gagccagaac cagatctacc
      721 tgtccttcct ttagcgcttt aatcaaatct tcagtagcat cccaggcagc aatttttacc
      781 actccagcta atccagcatt ggccactgcc tggtaagcac cctgggcgct atagaggttg
      841 gtaccaaaaa ttcctacgat atccgggtga gcctgcagag cagctgctac ctgttgctga
      901 gctttttctt gcacatccag gcagtattct actcccacta attcaatatc cggatattca
      961 gatattccct ttttgaaacc ttctactcgc tctactacgc tcgatgtatc agggttggta
     1021 gtttctacaa atacttttcc tttataatca atcatctccg ccaggtgatg agctacttcc
     1081 acccctccct gaaaattatc cgttccaata taagccaagg gaaatgacca gtcactgggt
     1141 ttagaataat ctccatcgcc gatgtaggta tcaacagtaa taatatcaat accactatca
     1201 taaatcctct tcaggggagc gattaaggct tcttgagaag taggtacgat ggcaattata
     1261 tcaatatctc ctcgggctgc caccgcttct aagataggaa cctgtacctc tggcccccag
     1321 gctttgggat gttcagcgat gaaagcgtcc atccctaact cggcggcctt gtctttaatt
     1381 ccctctccaa tcagaacctg gaaggggtca aggatacctg aaatgtatgc gattttgtat
     1441 tttttttctt gagctaaaac tcctccgact aacaccaata ccagtaaaga aacaatgaag
     1501 atagttaaga aattctttct catagcaaat cccccttctt ttataagatt ttaaaacttt
     1561 tttggttcct tctctcccct ttatttttca cctcctagag ggggaaagga taaccactta
     1621 gattataatt catcctttcc ctttttgcca acattttgaa attatttttg tagaaaaggc
     1681 tcttgtaact ggaactaaca aaaaatcatt gctgatgatt tagtacttct tgaccagagg
     1741 gaggtttttg ttattaaata taataaaatt tcaaaagcta aaaaggagga agatatgaac
     1801 aattttgagt ttttgagccc tactaagata gtttttggta aaggtacaga aaatcgggtg
     1861 ggacaggaaa ttaaaaaatg tcaggctgaa aaagtgttgc ttcactacgg tggcggaagc
     1921 ataaagcgtt ttgggcttta cgacagagtg gtggcttcac tcaaagagtc ggatattgat
     1981 tttatagagc ttggcggtgt gaaaccaaac ccccgggtga gtttggtacg tgagggtata
     2041 aagctgtgtc gggagagagg tgtagatttt attctggcag taggtggtgg aagtgttata
     2101 gattctgcca aagccatagg tataggagta ccatatgatg gtgatgtgtg ggatttttat
     2161 actggtaaag ctgaacctca agaaacaata cctgtaggtg tggtgttgac tataccggca
     2221 gcagggagcg aagcgagccc tggctcggtt ttaaccaacg aagatggatg gtataaaaga
     2281 gcggttaatg cagagattat gagacccagg tttgccatat taaaccccga gttaactttt
     2341 actctaccac cttatcagac agtatgtgga gcttcagata taatggctca tgtgatggag
     2401 cgttacttta ctcctacccg acatgttgac tttactgata ggctatgtga aagtacgctt
     2461 aagacagtta taaaaaatgt acctgtagtt cttgatcagc ctgataacta tgatgccagg
     2521 gctgagataa tgtgggctgg tacgatagcc cacaatgacc ttttgagcac tggtcgagtg
     2581 ggtgattggg catcacatga catagagcat gaaattagtg gcatatacga tgtggcccac
     2641 ggtgccggat tagcagtagt gtttccagca tggatgaagt atgtgtacag agaaaacagg
     2701 gataaatttg tccagtttgc agtgcgagta tggaatgtag atatggattt tgaggacccg
     2761 gagcgcacag cactggccgg aatagatcaa ctaaaaagat tttataagaa tgtgggactt
     2821 cccacttcgt taaaggactt aggcatatcg gatgatcgat tagaagagat ggctgataag
     2881 tgtacagctg gtggtacaaa gactgtcggc aattttaaga gattaggaaa acaagacgta
     2941 ttaaatattt taaaaatggc attgtagacc aatcaaagca tgggtgcttg cttgtctggt
     3001 cctatccatt ttgattttag gtggccttta ttgatgcggg cagtatagta tgtatttata
     3061 gtcgataaaa ggggaaagcg taaattttaa gtatagaggt acttatatct cttacgtata
     3121 gcctgcagtc ttttgaagcc cccagagctt ccctccatgg gctcaagttc gctccttctg
     3181 gagctatggt tattgagtgt tcttatgtgg atttaatcag ccctgaaaag aatccctatc
     3241 tgatgttcta gtcgaactat atttcctctt tcttgatatt caaatcgttt tttttttgtg
     3301 ataaccgtaa aattgccaac gaatgggaaa tgattcaaga gaatttatca attttatagg
     3361 gagcagaaca gcttacagtg aaactaaaaa cataaaaaat caccgagggg ggagaaagat
     3421 ctttctcaat atctgcagcg caaaataact tacggtgtga gataaaagaa aagggcttat
     3481 agaaagtgta aaagacaaaa actacgaaga agacgctttt tccctatcgg tgtgagggct
     3541 gccaccttat aacccctgga aaacaaaacg acaatagttt aaataaaaaa ataaagattg
     3601 ctgaggggag gtgctccagt gttgtgaggg tctcgctttt tagtcctcta tgctggtttt
     3661 agatacaaac actttacgca gaaaagcaag ggatgtctat ttttatttaa tttttttgtc
     3721 aactattcgg gaaaagaacc agagctttaa catctatcag agagccactt tctttcccta
     3781 tttttcttct aaagtctgtc tgacctttac ttttttcttt attgtggtaa aattctggaa
     3841 atttaagtga gttaagtaag ggaggaaaag taaatgcaat ctcttttcta tcctctggaa
     3901 aaggctggta ttcataaccc tcgctatgtt tactataatg cagttgtttc tcagctttta
     3961 gaagaagcca tcaaaagaga agaaggaatt ttcacttcta ccggagcttt taacgtgtta
     4021 accggtaaat atactggtcg ttctcccgaa gaccgttata tcgtagatga tgaaattacc
     4081 cataattcta tagattgggg taaaataaac gttcccatag aaccagaaaa attcaatcgg
     4141 cttcatggta aactatgtgc ttatctccaa ggtagagata tttttgttta tgatggtttt
     4201 gtaggagtag acccccaatg taggctggca gtgcgtttta taaatgaata tgcctaccag
     4261 acagtttttg tagaaaatat gtttcttgaa gcaacgaggg aagagttaac taattttaag
     4321 ccggaattta cggtaatttg cgctcccaat tttaaagctg accctgaaga agatggtatt
     4381 cgctctgaag cttttgttat catcaatttc cgggagaaaa tggtgattat tggaggtact
     4441 tcttacggag gagagattaa gaaatctatc tttacagtga tgaatttcct ccttcctcag
     4501 caaggggttc ttcctatgca ttgttcggcc aatgtgggag agagggggga cgatgtagct
     4561 atcttttttg gactttccgg cacagggaaa acctctcttt ctgctgaccc gagacggcgt
     4621 ttaattggag atgatgaaca tggctggtcg gaaaagggag tttttaattt tgagagaggg
     4681 tgttacgcta agtgcataaa tctctctcga gagaaagagc ctcagatttg ggaagctttg
     4741 cgctatggag ctattatgga aaatgtggta gtagaccctg tcactcgaga agcggattac
     4801 aacgatgccc gctatactga gaatactaga gctagctacc ccattgagta tatcccaaac
     4861 gctctcattc ctggtatggc tggacaccct gaagtagtga ttttgttaac tgccgatgct
     4921 ttcggagtga tacctcctgt agctaaactt agcagagaaa aggcggtgga gtttttctta
     4981 ctgggctata ccagtaagtt agcaggaacc gaaagaggta ttgttgaacc ccaggctact
     5041 ttttcacctt gttttggtgc tccttttatg cctcttcatc ctcaaatata cgctaagatg
     5101 ttaggagaaa aaatatcgca gcacggctcc cggacttacc tggttaatac tggttggatg
     5161 gggggtcctt atggggttgg taaacggata gatataactt atacgcgagc cattgttcat
     5221 tctttaatcg atggtagcat tgaaaattat cctttccggg aagaccctat attcggtttc
     5281 ttagttcccg aagaaattaa ggatgtacca cgagagctac ttcatcctcg ttctacctgg
     5341 aggaacggga gtgagttcga cgagcaagct agaaaattgc aaagcgactt tgcaaaaaca
     5401 attaaccgtt ttaagttctc tgcagtttga agtaagctca aatctgccat tactttaagc
     5461 tcaccagccg tagctggtga gctttttgtg tgcgcccggt atggacggtg gcttggtggt
     5521 tttgttttcc gggttgttat tgcgagctcg cagttttatc tggtaagtga cgattcaatc
     5581 ccacttagtt ttctcgctac aattggtaga tttcttcatc ggttaatagc tggaagccgt
     5641 ttctttccag tatgttatga gcttgaggtt gattttctac ctgtatgacc agaactgctt
     5701 tttccccgct tttgattaca aacccgtagg cgtcttcgat gttaatttga ttttcggcta
     5761 aagcccgggc tacccgatgt aacccccctg gctcatcatt catcactacg gcaatgacct
     5821 cttgcaggta aaccggaaaa ttatggttgc gaagaagctc aaaagctttt tgaggttcgc
     5881 tggctaaaat tttaatcact ccaaatccat tggcgctgga gatggtgata gcccgaatgt
     5941 tgattccttc ctgagaaaga aggcgagtaa ttttctcaat tcttcctggt ttattctcgg
     6001 caaaaatgga aagctggcga gccattttat tcctcctttt tataattgac ggtggtcaat
     6061 cactctttga acttttcctt ccggggcagg tatgctccct ggttctacta aaatcacctc
     6121 tggagttacg agaatttctg aatgaagctc agattgtatt ctttctctca atcttgctaa
     6181 tttttgtaaa tcacccagga atagctccgg ttttatttcc acttctacta tcattttatc
     6241 tcgataatct tctctttcta aaatgatgcg gtaattgttg cccacttcgg gattgctcat
     6301 taacactttt tctatctgca tggggaatat gtttactccc cggaaaatga acatatcatc
     6361 ggttcttccc tgaatacggg aaatacgccg gtgggttcta ccacaagggc agggttcctc
     6421 caggagagaa gctaaatctc cagttcggta gcgaataagg ggagtagcat cccgataaat
     6481 agtagtgaga actatttctc ctacttcccc gggaggtgtg ggagaaagag tttggggatt
     6541 gagtatttct aaaatgtaat aatcttccca caagtgcatt ccttcctgaa aaacacactc
     6601 aaaggctacc cctggtccgt tgacctctga taatccatag gaattataag ctttagctcc
     6661 ataaatttct tctatgcgac gtcgagtctc ttcgctgtga ggttcagctc ctaaaagcat
     6721 gatgcgaatg ttgaagtcct gtcgaggatc taagtcttca gctttgatta gttgagcaag
     6781 aagaagagca tagctgggga taatatgaat tgctgtactt ttaaaaaatt tcaaaaacca
     6841 gatttgtctt ttgctgtttc ctgctcctat aggaatggta agcgctccta acctctctgc
     6901 cccgtagtgc atgcctaaac ctccggtaaa aagcccataa ctcatcatat tctggaaaac
     6961 gtcatttttc ctcatcccta cagcatacat tgaacgagct acttgattag tccaccggtc
     7021 caggtctgaa gcagtgtaat atattactgt aggagtgcca gtagtcccag aagaggaatg
     7081 gaggcgtacc gcttcttcta aaggtacagc taagaaacca aaagggaaac cttctcgtaa
     7141 atctacctta gtggtagggg gaataaattt tagatcttcg ggagacttaa ttttttccgg
     7201 gtctattttg ttatgcgaaa aaagacgacg ataaaagctg gttctctgag cattagctat
     7261 ggtctttttg agcctttcag cttgtagtaa ggataattcc tcccggggta tagtttctag
     7321 ttgtttttcc cagtacaatt ttcttctctc ctttttttat ttatgtaacg tataatggat
     7381 taagacaatt atatctcact tgagaaaata tgaggaatcg gtataatcaa aagcgccttt
     7441 gcatatttat tggtagttac ttcaacgaat aaacattaag aatagctttg cgttgcgcat
     7501 tttctctttt ggaacggaga gagtaataat agaatagtaa tatgatgtgt ctaaaataga
     7561 gaggtgaatc aattgagttt agatctgaac gtctttatta gctttttagc tggtcttctt
     7621 tcttttcttt ctccttgtgt tttagctatt gctcctgctt atgtaagtta tattagcgga
     7681 gtagaatcaa ccgaaaaaga taggactaaa acgattactc atactctctt ttttgttaca
     7741 ggctttaccg ggattttcgt gttaatgggt ttaggagctt cttttttagg cagtatcatt
     7801 gctccttacc gggtgtggtt taatcgttta gcaggaatta ttattataat ttttggcttg
     7861 cagattttag gagttttgaa aattcgctgg ttatatgctg acaaacactt tcgcccccgg
     7921 gaaataaatc tatctcaagt gcgaagcctg cttctgggta tatcttttgg cctggggtgg
     7981 acgccttgtg tgggtcctat tctggggtcc atccttcttt atgcctctac tatggggaag
     8041 attgggcagg gaggaatact tcttttcttt tatgctctgg gcctggcttt acctttcatt
     8101 tttttgggaa taggctgggg acacctcacc acattttttc gtggtttaca aaaaagagga
     8161 agatgggtag aaataacgag tgggatattg ctcgttttcc tgggtgtggt aatatttact
     8221 ggagaaatga atgctctttt ttctctcctg ggggtggatg cttcttttaa tcctgaattt
     8281 ttgcttttca gagaatagga gtttttctta tgcctatcat tggtaaagat gaacaaaaag
     8341 taaaagcttt tggtgatgtt tctaaaagat taaatctctc taatcgagtt tatagaggta
     8401 tgcaagctat accagtggac aaaattgtgg gtagtgtggg aagagcagga gatttgctcc
     8461 ccggttttcg tctaaaaaac cccgatactc gttactatcg cattagaaga gcgatggaaa
     8521 gaggagaagt tctccctcct attattgtat atcaggtggg agatgaatat tacgtattag
     8581 atggtaatca tcgggtagca gtagctaagg agctgggtgt aaaatacatc gatgctgagg
     8641 taattgaatt tttcccttcc gagctaaggg tgagtcgagc cctgcgcaag aagagagcag
     8701 aatttgaaaa tttaaccgga cttaagggta ttgatgccag tgagcctcga cattatgatt
     8761 ctttcttaag ttatattaag gattatgcta agagtatgga gacattttta ggaagagaag
     8821 tacctcttaa agaagccagc cttaactggt ttctgtcggt ttatactcct gcagtccagg
     8881 ctattgtaga gagggggttg gaagaagcct ttcctgataa gagcttggct gacatttttg
     8941 cctatgttct tgactataaa tggtacaaaa gtcaaaaaga aggatacgat attggttttg
     9001 aacaggccct ggaaggtttt atccaggaaa tagtgggtaa acaagaaagc gaagaaaata
     9061 aaagaacttt tatgcaaaaa gtgggaggat tattagagga ggtattacct tggctgagaa
     9121 aggaataaac tggcacgagc tttctccgga agaagtaatc aagattttaa acagtaacga
     9181 aaaaaagggg ttaagcgagg aagaagtaaa acaaaggtta gaaaaatatg ggagaaatac
     9241 tctccctcag cccaggaaaa aaactatctt taccatgttt ctggagcagt tcaaagattt
     9301 cctggtattg attttagtgg gagcagcttt agtttctctt cttttgggag aaattactga
     9361 ttctttagta atcattgcta ttttgatttt gaatgctgtt ttaggagtgg tgcaggagtg
     9421 gagagccagt caagctctgg aagctttgaa gaaaatgact gttccggaat gcgaagtaat
     9481 aagagaggga aaacccctta aagtaaattc ggaagaaata gttcccggag atatagtgat
     9541 tctccgggaa ggagattttg ttcctgctga cttgcggctc attgaaattc atgccttgaa
     9601 aattgatgaa tcgagcttaa ctggtgaatc ggtaccggta gagaaagata tttctcctct
     9661 tcctctcgat attcctatca ccgaccgcct caatatggct tattctggaa ctctggttac
     9721 ctacgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa attgccgtcc
     9841 tctgtaacga tgccatagtg gaggaaggtg aagaaggtga agaggtgcgt cttggagacc
     9901 ccactgagct tgctctttta gattatgtct gggagcaaaa tgttgatttt aactcctgga
     9961 gaaaacttta tccccgccag gaggaggttc cttttgactc caaacggaaa atgatgtcca
    10021 ctctccacaa catagaagga gaaatggctc ttttagttaa aggtgccccc gatatagtta
    10081 ttactcgctg ctctttctat gagaaagaag gagaaattat ccctctttcc ccggaaaaag
    10141 agcaagagat aaagaaaatg atggaagaaa tggctgaaaa agctctccgg gttttagctt
    10201 ttgcttggag aaagatggaa gaagggattg taccttcccc ggaagaggaa aaagacctga
    10261 tttttgtggg gctaataggc atgattgacc caccccgccc agaggcgaaa ctagctttag
    10321 aagaagcaag acaagcaggc attactaccg tgatggttac cggtgataat cctctcactg
    10381 cccgggcaat tgcagaagag ctggggatgt tttctcctca ggacctggta gtcaccggac
    10441 aggagttagc taactattct ccggaggaat taaaagcgaa tattaaaaaa atcaaagtgt
    10501 ttgcccgggt gtggccagag caaaaactca aaattgtaga agctctacaa gctcacgaag
    10561 aagtggtagc tatgaccggt gatggagtta atgacgctcc ggctctaaaa aaagctgata
    10621 ttggcgtggc tatgggtatc tcaggtacag aagtggccaa agaaagcgct gatatgattt
    10681 taactgatga taattttgcc accatcgtgc aggctattaa agaaggtaga gtgatattcg
    10741 ataatatcag gaaatttgtg gtttatcttt tatcctgtaa tttaggagaa atatttacta
    10801 tttttattcc cattctggta ggttggcaca gtcctttagt accggtgcaa atcctcctca
    10861 ttaacctggt aaccgatggt cttccagctt tagccttagg gatggatagc cccgaacccg
    10921 atgtgatgat gagaaaacct cgccatcccc gggaggggat tgttaatccc cattatatgg
    10981 ggattgtact cttcaatgct ttctttatca ccttagcagt ggtagcctcc tttgggatag
    11041 gcttggcttt atggggacgg gagataagcc gaaccatagc ttttgccact ctgggttttg
    11101 acgagctatg gagagcttat agcttccgct ccgaaaagag aaatttctgg caaattgacc
    11161 ctcgaggtaa tttatatctg gtgggagcat gtttactgtc ttttttggta ataatactgg
    11221 tagtactttt acctcctctg cagggtgttt ttcaaacagc aactttaagc cttaatcaat
    11281 ggattttagt tgttcttttc tctatccttc ctgttagtgc ttatgaggtc agaaaattat
    11341 tccataaaaa ggaggaataa aaatatggaa gaaaaaatag tgaaaatcga gaatccccag
    11401 ggttataatt tgattctggg gcaggctcat tttattaaga ctgtggaaga cctttacgaa
    11461 gctttgataa cttcttctcc tcatttgaag ttcggtctgg ctttttgcga gtcttcagga
    11521 ccgtgtctcg tgcgttatgc gggaaacgat gaggagctca ccgaattagc caaaaataat
    11581 gcctggaata tatcggcagg acacactttc ataatttttt tgaaagacgg gtatcctatc
    11641 aatgttctca atactgtgaa aatggttccc gaggtatgcc acatatattg tgctactgcc
    11701 aatcctgtgg aggtaataat aaaagagact gaacaaggta ggggagtaat gggggtaata
    11761 gatgggttta aaagtaaagg gatagaggga gaaaaagaaa ttgccgatcg ccataaatta
    11821 ttgcgccaga tcggctacaa actttaagga gtgctggatg aagagagcca ctgctgacct
    11881 tcctcttcat gggggaaagt gccctccctg gctttttgag cggatgaaga ctttgggagg
    11941 actaattatg gaagctatag tagaattttt tggggaggaa gaaatactgt gtcggatttc
    12001 tgacccggtc tttttccagt ccctgggttg tctcttgggt tttgattggc actccagtgg
    12061 cctgaccact actctgtgtg gagcgctgaa agagggagta agaggcaaag aaaaagacct
    12121 gcgacttttc atctgcggcg gaaaaggagc aacttcccgc aagactccag aagaaatatg
    12181 ccttcatgcc cagaaacatg ccctcccttt tgctgaccaa atggtttacc ggagccgcat
    12241 gtcagccaaa gtagacaatt ctgctctcca ggatggctac aatctctatc accatacttt
    12301 cttttttacc ttctccggaa aatgggcagt ggtgcagcag ggtatgaatc ctgacactaa
    12361 atacgcccgc cgatatcatt ggtggggaga aggagtaaaa gatttagttt gtgaacctca
    12421 ccagggaatc tggggagaga aaaaagaaag ggaagttctc aatctggtag ctcgagagag
    12481 cacagattgc cgagagggta tagtaaagtt agcccaggaa aagccggaaa aattaaaaaa
    12541 ggagatggcc cttgttcccc acctttcact tccttctcgc cacttcatca aggaagaaga
    12601 ttttaatctg ggacgaatag ataaaaccct gcatatgatt tacgaggaaa agcctcaaaa
    12661 ctttgaaaac ctcctggctc tccggggagt gggccctaaa acnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nntcggccaa caattctcag ccctgggaat ttatagtggt
    12841 aaccgatcct gataaacgga gggaaattgc taatgccact gattatggga aatttattgc
    12901 tcaatctccg gtttgcgtag ctgtattttg ccgagacact catttttacc tggaagatgg
    12961 ttgtgctgct acggaaaata ttcttcttgc tgctcgagat ttaggtttag gttcttgctg
    13021 ggtagccggt gataaaaagc actatagtcc acagatagct gaaattctca atgtcccgga
    13081 tgattatcgt ctggtaagct tgatagccct gggatatcct gcagtagagc ctgctatgcc
    13141 aacaaaaaga agtttagaag aagttttaca ctgggagaaa ttttgagagg gataacaatg
    13201 aaagtgggta ttttatccga cagtcatgat aatcttgcct cgctaaggca agctctggat
    13261 attttagaaa aggagaaagt agacctaatc cttcatgctg gagactatgt tgcccccttc
    13321 tcagtagaac ttttaactcg cttttctatc ccctgggagg gggtactggg caataacgac
    13381 ggagagatac tgggcatttt tcagaaatca ggagggaagg taaatgctcc tttctgggag
    13441 ggagaaaaga atagtttccg catctggatt tcccattttt atttgccggc gcaattagcc
    13501 tgggaaagta gaaaatacaa cctggtagtt tacgggcata cccatgaagc ggttataaaa
    13561 gaggaaaacg gcgttttctt gattaatccg ggggaagtgt gcggtctact cagcggaaga
    13621 gctactttgg ctctttgtga tttgaaagaa ggagtagcaa aattaattga tatttgatat
    13681 ttgattaaaa ttgagaaggg ggataacagt ggaaatcatt cgggagcgtt tcacccaaca
    13741 gttaaaagag ctggaagaag atttactaaa tatgactcac acagcagcga aaatgttaca
    13801 gatgtctatt gagtctctaa aagaaagaaa tatagaaaaa gcagaagaag ctattgcttt
    13861 agatgatgta gtggatgact tcaactttaa aattgaagaa aagtgcctat cactcattgc
    13921 tttgcaacaa ccagtggcta aagatttacg agtaattgct gccatactga aagttattac
    13981 tgatgtggag agaattggtg actattctgt tgatattgcc aaattcagca ttcgcttggc
    14041 cgaaaaaccg ttatttaaac ctctcataga tgtgccaaaa atggcagact tggtaaggaa
    14101 aatgttagag gaggctatca ctgctcttgt gcaaagagac ttaaccatag tacaaaaagt
    14161 ggtggaagcc gatgatcagg tagatgctct ttatcgttct gttcatgaag aagtggtaga
    14221 gcacatagaa aaagacccca gtgttactcg gcaggctatc tggattttga tgattgctcg
    14281 ttacttagag agagttggag accacattac taatattgct gagcgggtct actatatgga
    14341 aaccggagaa ttaatagaat tacatcagta gggggaaaaa atggaagaga aaaaaatctg
    14401 gtattttcct cagtccggtc cagataacac attagccact ctggacattg ctctagaaag
    14461 ggcaaaggag ctggggataa aacatatggt ggtagcttct cgctctggag atactgcttt
    14521 atctctggaa aagaaagccc gagaggtaga ctactctggt aatttagtta tggtaactta
    14581 tcatgctggc ttctgtggcg aagatagcat atcttttact ccttcccgga gagaagagct
    14641 ggaaaaaaag ggagtgaaaa tcgtcatgag ctctcatgct ctaagcggag ttggtcgctc
    14701 tttccgacag aaatttggag gggtgagtat cccggaaatt attgctgaga gctatcgacg
    14761 tatttcccag ggctttaaag tggcagtgga gatagctatt atggctgccg atgggggagt
    14821 tattcccacc acagacgatg ttatctctat gggaggaaga ggcaaaggag tggataccgc
    14881 gattctcctc agagctgctc atcaaaattc ttttttcgat ttaaaaatta aagaaatact
    14941 ttgtttaccg cgagaagaaa aaacttagaa aggaggaaat ctaatgatta acacttttaa
    15001 agctactgga gagtggaaag gaggcctacg gatagatact caggtgagga attttactct
    15061 ttcctttgat gaaccacctt ctctaggtgg agaagatagt gcctgtaacc cggtggaggg
    15121 tgtactggct tctctcgccg ggtgtctggg gattgtcagc cgggtggtag ctcaggagaa
    15181 aaatttaccc atcgagaaaa tagagataga ggtagaagga gatttagacc ctcgaggatt
    15241 tatgggacaa tatgatcagg ttcgtcccgg actgttagct attcgctatc aggtcaaagt
    15301 gaatggtgac cttagcgatg aagaattaaa agagtttgaa gaagaggtag acagacgctg
    15361 cccggtatcc gatactttaa aaaatggcac taaagtagag gggagagtga ctttagagca
    15421 gtctacggcg tgagattaaa agcagaatag ataaaaaaaa tataaggcac tgagggacgc
    15481 ttttcctctc agtgcttgca gtacaaagtt aaaagcaaga tagatggaaa aaccaaacac
    15541 aaagacacgg ggttaatact tcagtgtgcc tgcgtgttcc tatcgagggt ttgaactata
    15601 ggggaattag ttcccttgta actccctgga agacaaaaac acaaggatta aaaataaata
    15661 atattggaaa gaaaaacaaa cagcttacga cttgagataa agatatataa aaaatcagga
    15721 atgacggaaa aggggagtga cagaagaaga taaattcctg gctaaattcc gggaatgaca
    15781 taccaaaatg acagcaggat aatttctgtg gcttctgaag aagaggaagt ataaataatc
    15841 caccagggaa ggaaaagagc tttctcggta tagtagaaat tcccggcact gctacagctt
    15901 attcgtagca caaagttaaa gagggagcta cgtataccac cgaagagaca gtctttctat
    15961 tccctggtat atatagttag ctataaaaat agtacaggaa gaaatgtgtt ttcttcaata
    16021 gaaaggagat tatgatgaaa gctgctattt tagaagatat agatcgctta gtggtaagag
    16081 aagtagagga ttatgaggta gggaaaggag aggtgctgat aagggtcaaa gcctgtgcca
    16141 tttgtggttc agatatccga attatgcacc atggcaatcc ccgggttaag tttccccata
    16201 tcatcggtca tgaaatcgca ggtgttgtgg tagaagtggg tgaaggagta gaaaaattcg
    16261 agataggaga tcgagtggca gtgggggcag acgttccctg cggaaaatgt ttctggtgtc
    16321 agaacggaat gggaacaaac tgtgctataa actatgctat tggttatcaa ttccccggag
    16381 ggtttgctca atatattcta cttaattcct tatcagtgga acagggatca gtgcacaaaa
    16441 tccctgaaaa tctttctttt gatgaagcgt ctttagctga acctttggcc tgtgccatta
    16501 acgggctgga gatggctaat ctggaagtgg gagacactct atgtatcatt ggagcaggtc
    16561 ctattggctg tatgatgata gaaatgggcc gtcatatggg ggcaactaat atcatcgtta
    16621 tccagcgttc tcagctgcgg ctggatttgg cccgtaaatt taatgccgac cattattttt
    16681 tgagcgatag acatgtggaa gagaaggtga aagaggtaac agatggagaa ggagcagatg
    16741 tggtgatggt gacctgtgcc tctcctgaag cacaagagca agctcttctt ctcgctcgcc
    16801 atcgagcccg gataaacttt tttggtggat taccttccgg ggcacgtaat ataagcattt
    16861 ctagtaacct tatccattat caggaatgta tggtgttggg tagccatggc agtctccccc
    16921 gtcatcatca aaaagccctg cgggtaatgg aaaagggaat ggtaaatccc gcagagtata
    16981 ttactcatcg cttccctcta gaggaaattc aggaagcttt tagagtagct gaatcccacg
    17041 aagggctgaa agttgtggtt aatccatgaa ggctctggtt ctggaagaaa aaggagtttt
    17101 gtctctcagg gaggtaaaga cccctgtccc tcgggaggga gaagtcttaa taaaagttcg
    17161 ggcggcagga atatgtggct cagacctccc ccggattttc ggtggccagg cttattttta
    17221 ccctctcatt cctggccatg agttttcagg tgaagtagca aaagtggcag aggaagagga
    17281 tgaggaatgg ttgggagaaa gagttacagt ttatcctctt cttccttgcc gagattgcga
    17341 atattgtgaa gtgggtcagt atgagttatg taataattac ggatatttag gctctcggca
    17401 aaatggaggt tttgccgagt ttgtagtagc tcctgtagag aatctgcttc ctctgcctac
    17461 cagcgtttcc tgggaagagg gagcgctgag tgaaccagga gcggtaactt tgcacgcctt
    17521 gcgacgtgct cacccggagg tgggtgatag ggtggcagtt tttggtttag gccctattgg
    17581 gcttctctgt ggcatgtggg caaagttagg aggagctcga atgctcacag gatatgatgt
    17641 ggatgagcaa aaatttcc
//
LOCUS       DAOPSS010000022        17468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2736_length_17468_cov_8.705967, whole genome shotgun sequence.
ACCESSION   DAOPSS010000022 DAOPSS010000000
VERSION     DAOPSS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 17468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17468
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_17468_cov_8.705967"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PKN31_02565"
     CDS             complement(<1..845)
                     /locus_tag="PKN31_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98846.1"
                     /translation="MRKITFSLLIFLVLLLFFFNLPGGAQDLEKEISEQQAKLEKLRS
                     EQLKLDRELDKLLKDEKNLVKEIANLDKQIENLEKNAAQSRAKILQLEAERAKLQKEI
                     EVLSQKIEEDKGKVKDILVRAYKYNGGVSFWELLMTTSHPAQWEEQWFLLRKYYKSQA
                     DNLERYLGEKETKEKKEEELAQMIRLELVLKEQALLENEKLAKLKDTREKMRQKIASD
                     KKQFERTKNELVKAQKEMESLIAKLQEDLKKKREGQPPISITPVKQGRLFWPVQGTVV
                     EGFGER"
     gene            complement(842..1711)
                     /locus_tag="PKN31_02570"
     CDS             complement(842..1711)
                     /locus_tag="PKN31_02570"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOA98847.1"
                     /translation="MPKSKEAFHHRGTSYIVMGFLSIFITQLLLNLFIFGYLELEQMG
                     GVWQSSFTIRAFFPEEVSPEVVEDVKERVKALPLVGEVKLVDSEEAKSRFLSYFNLQE
                     EDLGLEENPFPPSLEVQAQRVEELPFLAQELEKIEEFEEVFYGGENTDALLRFYRFFL
                     TAGGLLLLALFVFSLLVVTTVIRASIQARETEIEVWNLMGATKRFIRAPFIYQGVMEG
                     LSGGVTAFVVSLVFFHFLLDFLNTSFPLFFWINWEELILPLGVVSILMSSFLGFLGAL
                     LACNSIEEESAKK"
     gene            complement(1724..2389)
                     /locus_tag="PKN31_02575"
     CDS             complement(1724..2389)
                     /locus_tag="PKN31_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA98848.1"
                     /translation="MGFLEFKKVYKVYPAGVKALDNVNLSIEKGEFVFLWGATGAGKT
                     TLMKLITREELPTSGEVWLERCKISSLEDRYLPILRRNLGVVFQDLKLVPHWTVFENL
                     ISPLLVLGVKKRDAHRLAEGILDLVHLQDKKHHKTEWLSGGEKQKLCLGRALIHRPHL
                     VLADEPTGNLDGATALGLVGTLFEFSERSGATVIIATHSEKLVNRFPGRLVVLDKGKI
                     IQS"
     gene            complement(2416..3156)
                     /locus_tag="PKN31_02580"
     CDS             complement(2416..3156)
                     /locus_tag="PKN31_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOA98849.1"
                     /translation="MKITTRELFGLSFYDCSLPELLEVLEDKIEKKEKTHVITANPEI
                     LARGFQEEELNKVLKEAEIVVADGIGVVMASRFLGEAISHRLPGVEIGEALMMQAEEK
                     EWRFYFLGGREEVVEEAVKELKEKYPHLEIGGFHHGYFGDNDEEVIKDINRASPDILF
                     VGLGSPRQEYWIRENRERINALVMMGVGGSFDVWSGTKKRAPRWIRKLNLEWAYRIIC
                     EPQRLQRVVPAFFNFARLVWKEKRDPTV"
     gene            complement(3153..4190)
                     /locus_tag="PKN31_02585"
     CDS             complement(3153..4190)
                     /locus_tag="PKN31_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOA98850.1"
                     /translation="MLGYYGFGNWGDELSLRAVLGDLERIREELSLPFFYQVLYRESD
                     FLLPLPENAFPLERDSFFSVLQAIKSSDYILVGGGSLLQDVTSFRSLLYYFSLLRWAQ
                     LWNKKVIFYRVGLGPLIRRESRFLVSRLLRKSSLSIFRDEESLNLALDLGAPSSFLGA
                     DPLFSWGGKEKKEEGEKVALFVRSFPEENREALYRAFLRFSKVEEKRLEIVAFHPEKD
                     REIAKDIAQYLRCPCVILSQFSQIIPYFEKLKAVFTLRLHPAILATLLDIPWFALDLD
                     PKIRAFSRWWGEENLLSFSDLEENRLEEVYQRRDILRERGKEVKRELKQKSEDSVKIL
                     GEFLKNAEKVR"
     gene            complement(4198..6072)
                     /locus_tag="PKN31_02590"
     CDS             complement(4198..6072)
                     /locus_tag="PKN31_02590"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOA98851.1"
                     /translation="MRKNIFLLVGFLIIFFFSLPILSERWERESQSRVVELVVDWNDL
                     EWVGKTEGVDPLLLAEELKKEGFNSLGISELTWRQLVSQGRAVPVAPSPFSSPILRYF
                     QISDPLLREAVKTHLLLLGKEVEEKGEILGVNILYEEEDKIGVGWDYRILQALQEQGF
                     RLILRPYNRYNIPPSVLESLLEEKAWEMTEGIIFSGDSVLGAPRTDSLRFLSSFLEEK
                     GLFWGYLEFVGQKGETTLASLAPHFTLRAHSIPPEELKGYTPGEARDRFLRAVKERSV
                     PILYLRLFTDPPAFESWEKNLSYLLSVKKELVDNGYTPGEVSVPSTSFSPPLYSLLFF
                     SLALSFFAFYLWGSFFRPSFWIIPLCFIPLAGLIFYNPLWGMRVLGLLAGIVYPTSGV
                     IILIDAISSGKGKLQSLGWAFMTLFSGALTVSMGLYHWLFLLRIYQYWGVKVSLIAPP
                     LLVLFYLFKKSYWGVSIQQVLLEDLKRFELLIVAFLGIAALLYLTRSGNFPLLPAGGL
                     ETRIRSLLERLLFMRPRTKEFLIGYPALWLLFYWDKDKVRPAYSLILWLGVSVGFATF
                     FNSFCHIHTPFLYILLRFLNALVLSLPVAVIYIVVIKLALFLWQRIGRWGERGHSAEN
                     "
     gene            complement(6069..6425)
                     /locus_tag="PKN31_02595"
     CDS             complement(6069..6425)
                     /locus_tag="PKN31_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOA98852.1"
                     /translation="MRRFLVRVNGEEYEVEVAEVREEGESHWNIRDIKKVVPSPSPLQ
                     VTTTLRAPMPGRVLAINVAKGEKVEAGQVVIILEAMKMENEIAAEREGIVKEVYVEEN
                     DTVDSGDPLLEIEEGR"
     gene            complement(6429..6587)
                     /locus_tag="PKN31_02600"
     CDS             complement(6429..6587)
                     /locus_tag="PKN31_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98853.1"
                     /translation="MKLKPQVVAAIAAALFEILEEEKPSLPYVPKKNKKWKELGKHER
                     CWMLRLGR"
     gene            complement(6595..8154)
                     /locus_tag="PKN31_02605"
     CDS             complement(6595..8154)
                     /locus_tag="PKN31_02605"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOA98854.1"
                     /translation="MSKKTLLELVEELEKEKALLLRGGGEKALKKQHEAGKLTARERI
                     ELLVDEGSFEEIDLFIEHRADRFGMAERKLPADGVVTGFGTIEGRVVFVYSQDFTVMG
                     GSLGEMHARKITKIMDMALKVGAPIIGINDSGGARIQEGIDSLSGYGQIFFRNTQASG
                     VIPQIALILGPCAGGAVYSPALMDFIFMVEGESKMFVTGPQVVKAATGEEISAEDLGG
                     ATTHATKSGVAHFVASTEEECFLKVRTLLSYLPSNNVEDPPVGDSSDPWNRHNTPLLE
                     IVSPHPNKPYDMKEVVEELVDRGSFLEVQKDFARNIIVGFSRIGGQVVGVVANQPYHL
                     AGCLDINAADKASRFIRFCDCFNIPLLTLVDVPGFLPGSNQELGGIIRHGAKMLYAYS
                     EATVPKITVILRKAYGGAYLAMCSRDLGADLVLAWPTAEIAVMGAEGAVDIIFRQEIE
                     KALDSEVEREKLLEQYRQEFYNPYQAARRGYVDEVIDPRETRIKVAKALEVFWSKREE
                     GKGKKHGNFPV"
     gene            complement(8147..8974)
                     /locus_tag="PKN31_02610"
     CDS             complement(8147..8974)
                     /locus_tag="PKN31_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOA98855.1"
                     /translation="MKVYALIGPSGTGKSYHVPHLAQKYKIDCIIDDGLLISGGRIIA
                     GRSAKAEINKIRATKIALFYFSPDREAVKEALRKLAPQNIMILGVSLRMVEKICEHLE
                     LPLPEEVIEIEKIVSPQEIELALSARENEGKHTIPVPLVELKRNLWSSLMDTVPVAIW
                     RWFYSSREKTVVRPPFSYLGKLVISEKALLSLLDFLLSRFPWIEEVKGLSIHYSERGV
                     EIVVNCIINPDFSLSQVGHLTQKRLKEGLEYLTGVDVRAVNVNVEGIADREEQLVYV"
     gene            complement(8980..10084)
                     /gene="prfB"
                     /locus_tag="PKN31_02615"
     CDS             complement(join(8980..10020,10022..10084))
                     /gene="prfB"
                     /locus_tag="PKN31_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143156243.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOA98856.1"
                     /translation="MPDVIEELEEVKGKLEEIGGYFDQLNLEEKIREIEKKMSREGFW
                     DNRGGENQILGEYKSYRQIWEQYQNLQERWREAQEFSSLISETDSEFSELKKEIKSLT
                     QEAEDLKIRLLFSETEDSHNAIVTIHSGAGGVESQDWVEMLFRLYLRFCERKGFEVRV
                     TDILEGEEAGLKYVTFLVQGDYAYGYLKSERGVHRLIRISPFDANRRRHTTFALVEVT
                     PEMGDDIKVEIEPQDLKIETFRAGGHGGQHVNVTDSAVRIIHMPTGIVVQCQNERSQH
                     QNKALAMRILKSRLYEYYQEEQKKKIMEMKGERKDIAWGNQIRTYVFHPYSLVKDHRT
                     GHETGNVERVMNGEIDDFVEAFLEKSQISSEKE"
     gene            complement(10101..12779)
                     /gene="secA"
                     /locus_tag="PKN31_02620"
     CDS             complement(10101..12779)
                     /gene="secA"
                     /locus_tag="PKN31_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOA98857.1"
                     /translation="MGWIKDFWADRAQAKQIRELKDKYVEAVNAQEKEVSRLSDAELR
                     HKTEEFQRRIQEGSSLDDLLVEAFAVVREVAKRTTGMRPFDVQIMGGVVLHQGKIAEM
                     ATGEGKTLVATMPAYLNALEGKGVHIVTVNDYLAKRDRYWMGPIFEFLGLSVGLIQHD
                     SSPAERKKAYLCDITYGTNTEFGFDYLRDNMVIRFEDVVQRELNYAIVDEVDSILIDE
                     ARTPLIISGPAEEDTEIYYKVNKVVRRLSYPEDFDYEEKTRSIWLTEKGVTKVENLLH
                     IDNLYEETGKGSLEHLVRQALRAHHLYKKDVDYVVKDGEVIIVDEFTGRLMHGRRYSD
                     GLHQAIEAKEGLKVANENQTLASITYQNYFRMYNKTSGMTGTAKTEESEFIYTYGMPV
                     VVIPPNRPLHRTNHPDVIYRREEEKFAAVVEEIEKQNKAGRPVLVGTVSIEKSERLSN
                     LLHQRKIPHQVLNAKHHEKEAEIVAQAGKKGAVTISTNMAGRGTDILLGGNPDFLARE
                     EMRSKGFKEEIILEAAEGLPTDLPEVKEAREVYRELLSRFKEITDREGEEVKAAGGLH
                     VIGTERHESRRIDNQLRGRAGRQGDPGSSQFFLSLEDDLLRLFGSDKISQLMEKLGME
                     EGVPLEHSLVTRAIESAQKRVEGHYFQIRKTLLQYDDVMNKQRGVIYEQRRMVLREEN
                     LRPHILGMMEEVVKDIVSAYAPEKVYPEEWDWEGLSQRLFSVLGWEWKVDEEKKLSYY
                     PSLLQEDILQSLREIYESKVQEIGEPLFRELERYVLLRVVDSYWKDHLRNMDHLREGI
                     GLRAIGQRDPFVEYQLEAFDMFQDMIAHIREDTIRYILHLRVVTEAKKPSSRASSPKP
                     PREKVATARSKNKVGRNDPCPCGSGLKYKYCCGKNE"
     assembly_gap    13218..13317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13678..14469)
                     /gene="rsmA"
                     /locus_tag="PKN31_02625"
     CDS             complement(13678..14469)
                     /gene="rsmA"
                     /locus_tag="PKN31_02625"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOA98858.1"
                     /translation="MTSFHISPRWGQHFLVDKAVRDIIIDKADLKKEDTVVEVGVGKG
                     ILTEVLVQKAGKVIGWEIDERLYRMVREKLKDYSNLVLYREDFLSADLASLLSSFSSL
                     KLVSNIPYAISSPFFSKILESGIKWDLLVLMVQREFGEKVLQKKGSPLAVGVNLLYEV
                     EKVREVSPGSFSPPPAVRSLILKFIPLSPQIEPDAYKEIMRWVHFLFSQRRKKVEKLL
                     RPQLGREKTREILARAHIHPEWRAEHLEIEHWIALVSQLETIANS"
     gene            complement(14502..14687)
                     /locus_tag="PKN31_02630"
     CDS             complement(14502..14687)
                     /locus_tag="PKN31_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98859.1"
                     /translation="MYNPEGLCQVCRHCDAEMKKKGIYKCLLGEQKERQEPVDRCDYF
                     FPGHLEHPEENYSMLNP"
     gene            complement(14711..15058)
                     /locus_tag="PKN31_02635"
     CDS             complement(14711..15058)
                     /locus_tag="PKN31_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOA98860.1"
                     /translation="MPFSEEPGEIRVSVSTLVEMAARSAFLVEGVMGDFNHLLRNTKV
                     VVQGETLSPNDRVSTKEKGVFINISLKVDSRKNWEEIAQEVQKRVANMIREWGFPLEG
                     VNVEVSEVEWFLE"
     gene            complement(15048..15539)
                     /locus_tag="PKN31_02640"
     CDS             complement(15048..15539)
                     /locus_tag="PKN31_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOA98861.1"
                     /translation="MNHNWIIGVIGQHSCSAELYESAYRVGKGIAERGAILLCGGLGG
                     VMEAACRGAKEAGGFTVGILPGNSREDANPYVDLKIVTGLGEARNLIIVLTASALIAC
                     GGQSGTLSEIALALSHDKILAGLNTWKIQDSTGKEDFFPQFQDAKEALHYVWEKLESN
                     NAL"
     gene            complement(15529..16020)
                     /locus_tag="PKN31_02645"
     CDS             complement(15529..16020)
                     /locus_tag="PKN31_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOA98862.1"
                     /translation="MIKMKVQGLMFDQKNTMAVVVLSDEEEKRSLPIWIGLFEAQAIL
                     LGLQGVKTPRPLTHDLMASLIHQLGGELEKIVITRIVDNTFYALLYIQVEGNEITIDA
                     RPSDGIALSLRMGAPIFASEEVLSSTTVATGEIDDKEIEEFRQFLDNLKPEDLKKYLG
                     YES"
     gene            complement(16041..16835)
                     /locus_tag="PKN31_02650"
     CDS             complement(16041..16835)
                     /locus_tag="PKN31_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOA98863.1"
                     /translation="MSFWVDIHAHLEGEEFLKDREEVINRAQNAGVEIIVNAATSLSS
                     IQETLKLVERYPSLYASVGIHPQEIGDELPDFSILEEYVRKEKVIAIGEVGLDYYWDR
                     SHIEKQKEAFIQQIEVAEKFSLPLIVHSRESNKDLWQIIKEKVRDVVVIWHCFSGDEE
                     ILQEALPRAVYFSLGGVVTYPGATHLREVVQKIPLSRVLLETDAPYLPPQSRRGRRNE
                     PAFLVETASFLAQLWGVSLLTLREKVWENFCSIFGRKLDLRYNNKT"
     gene            complement(16832..17167)
                     /gene="metG"
                     /locus_tag="PKN31_02655"
     CDS             complement(16832..17167)
                     /gene="metG"
                     /locus_tag="PKN31_02655"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOA98864.1"
                     /translation="MVLPEINLEDFQNIDLRVGEVVKAERVEGTKALLALRVNLGEEE
                     RTLVAGLANYYTPEELMGKKIIVLTNLKPANIRGIKSEGMLLAADDGKGAVSILTVDR
                     DIAPGSKVR"
     gene            complement(17181..>17468)
                     /locus_tag="PKN31_02660"
     CDS             complement(17181..>17468)
                     /locus_tag="PKN31_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOA98865.1"
                     /translation="HFANRYIEREAPWKLHKEGKEKEVENLLYRLLDNARLLSFFLYP
                     FMPDTTLKLRELLGLQQDPGKESLEESLCWQGGPEVYSVEEAQPLFPRIIE"
BASE COUNT         4973 a         4447 c         2928 g         5020 t
ORIGIN      
        1 cgttctccaa aaccttcaac aactgtgccc tgcaccggcc agaaaagacg cccctgtttt
       61 actggagtta tagaaatcgg gggttggcct tctcttttct tttttaaatc ctcctgcagt
      121 ttggcaatca agctctccat ctccttctgg gctttaacca gctcattttt agtcctctca
      181 aattgttttt tatctgaggc aattttctgc cgcattttct cccgagtgtc ttttagctta
      241 gccagtttct cgttttctaa aagcgcttgc tcttttaata ccagctctaa acgaatcatc
      301 tgtgctaatt cttcctcctt tttttccttt gtttcctttt ctcctaaata acgttcaaga
      361 ttatcggctt gagatttata atatttacgg aggagaaacc actgctcttc ccactgagca
      421 gggtgactgg tagtcatgag caactcccag aaactaactc ccccgttata tttataagcc
      481 cggactaaaa tatctttcac ctttcctttg tcttcctcta ttttctggga aagaacttca
      541 atttccttct ggagttttgc tctctccgct tccagctgga gaattttggc ccgggactga
      601 gcagcatttt tctccagatt ttcgatttgt ttatctaagt ttgctatctc tttaaccaga
      661 tttttttcat ccttcaataa tttatctaac tcccgatcta atttcaactg ctcgcttctc
      721 agcttctcca actttgcctg ctgctcggaa atttctttct ctaagtcttg agctcctccg
      781 gggagattaa agaaaaataa aagtaaaaca agaaatatca gtaacgagaa agtaatcttc
      841 ctcatttttt agcactttcc tcttctatcg agttgcaagc caaaagtgct cccagaaacc
      901 ccaaaaaact gctcatcaaa atacttacca ctcccagtgg caaaatcagc tcttcccagt
      961 ttatccagaa aaaaagagga aaagaagtat taagaaaatc gagcaagaag tggaaaaaaa
     1021 ccaggctaac tacaaaagct gtcactcctc cacttaaacc ttccatcact ccctgataga
     1081 taaaaggtgc tcgaataaac cgtttagtgg ctcccatgag gttccagact tcaatttctg
     1141 tttctcgggc ttgaatagaa gctctgataa cagtggtaac cactaaaagg gaaaaaacaa
     1201 aaagagccag aagtaaaagc cccccagcag taaggaaaaa gcgataaaag cgcagaagag
     1261 catcggtatt ttctccccca taaaaaactt cttcaaattc ttctatcttt tctaactcct
     1321 gagccagaaa gggcaattct tctacccgtt gtgcttgaac ttccagagaa ggaggaaaag
     1381 gattttcttc taaaccgaga tcctcttctt gcaggttaaa atagctcaaa aatctacttt
     1441 tggcttcctc tgaatcaacc aactttacct ctcctacaag aggcagggct ttgaccctct
     1501 cctttacgtc ttctactacc tcaggagata cctcttccgg gaaaaaagct cggatggtaa
     1561 aactactctg ccacactcct cccatctgct ccaattctaa ataaccaaaa atgaaaaggt
     1621 ttaaaagcag ttgagtgata aaaatactca agaatcccat cactatataa gaagttcccc
     1681 gatgatgaaa ggcttctttg cttttaggca aaactcattc tccttaagac tggattatct
     1741 tacctttatc taacacaact aaacgacccg ggaatcgatt taccagtttt tcgctgtgag
     1801 tggctataat cactgttgct cctgacctct cagaaaattc aaacaaggtt cccaccaaac
     1861 ctaaagcagt cgcaccatcc aggtttcccg tgggctcatc agccagaact aaatgaggac
     1921 gatgaattaa agctctaccc agacataact tctgtttttc cccgccggaa agccattctg
     1981 ttttatgatg ttttttatct tgtaggtgca ctaaatccag aattccttcc gccaggcgat
     2041 gggcatctct ctttttcact cccagaacca gaagcggaga aataagattt tcaaaaaccg
     2101 tccagtgagg caccaatttc aaatcctgaa aaacaactcc taaattgcga cgcaaaatag
     2161 gtagataacg atcctccaga gagcttattt tacatctttc cagccatacc tctcccgaag
     2221 ttggtagctc ttccctggtg ataagtttca tcaaggtagt ttttcccgct cccgtggctc
     2281 cccagagaaa aacaaactct cccttctcta tggaaagatt tacattatct aaagctttta
     2341 cccccgcagg gtaaacttta tagacttttt taaattccaa aaatcccatc ttacttcctc
     2401 ttgttcgata ttatactata ccgtcgggtc tctcttctct ttccatacta atcgagcaaa
     2461 attaaagaaa gcgggaacaa ctctttgcag acgttgtggt tcacaaatga tgcgatatgc
     2521 ccactccaga ttcaatttcc ttatccatcg aggggctctc tttttagtcc cgctccacac
     2581 atcaaaactt cctcccactc ccatcatcac cagagcattt atcctctctc gattttccct
     2641 gatccagtat tcctggcgag gggatcccag acctacaaat aggatatcag ggctggcccg
     2701 attgatatct tttataactt cttcatcatt atctccaaaa taaccatgat gaaaaccgcc
     2761 aatctcaaga tgaggatatt tttctttcag ctcttttact gcctcttcta ccacctcttc
     2821 tcttcccccc aggaaataaa atcgccactc tttctcctcc gcttgcatca taagagcttc
     2881 tcctatctct actccgggca aacggtggct tatcgcctct cccagaaaac gggaagccat
     2941 caccactcct ataccatcag ctaccacaat ttctgcttct tttagcacct tattcaactc
     3001 ttcctcctga aatccccggg ccaaaatttc cggattggcg gttattacat gcgttttttc
     3061 ctttttctct atcttatctt ctaacacttc taaaagctcc ggcaaagaac aatcataaaa
     3121 agaaagaccg aaaagttctc gagtagtgat tttcatctca ctttctcagc atttttcaaa
     3181 aattctccca gtattttcac actatcctct gatttttgct tcagttccct ttttacctct
     3241 tttcctcttt cccttaaaat atcccttctc tgataaacct cttccagcct attttcttcc
     3301 aggtcagaaa aggaaagaag attttcctcc ccccaccacc gagaaaaagc ccggatttta
     3361 gggtctaagt ccagggcaaa ccagggaata tccaacaaag tagccagaat tgccggatgc
     3421 aagcggagag taaaaactgc ttttaacttt tcaaaataag ggattatctg agaaaattga
     3481 gaaagaataa cacaaggaca tctcaagtat tgagctatat ctttagctat ctccctatct
     3541 ttttccgggt gaaaagccac tatctccagt cttttttcct ctaccttgct gaatcgtaaa
     3601 aaagcccgat ataaagcttc cctgttttct tccgggaaag agcgaacaaa caaggctact
     3661 ttctctcctt cttccttttt ttcttttccc ccccaggaaa aaaggggatc ggctcccaaa
     3721 aaagaagaag gagctcctaa atctaaagcg agatttaagc tttcctcatc acgaaaaatg
     3781 gagagagagc tttttcttag aaggcgagag accaaaaaac gactttctct tcggataaga
     3841 gggcctaagc ccacccggta aaaaataact tttttattcc acaactgtgc ccagcgcaaa
     3901 agggaaaaat aataaagaag agaacgaaaa ctggtaacat cctgcaggag gcttcctcct
     3961 cctaccagga tataatcact acttttgatt gcttgaagaa cagaaaaaaa actgtctctc
     4021 tccagaggaa acgcattctc aggaagagga agtaaaaaat cgctctctcg gtacaaaacc
     4081 tgataaaaaa aggggagact tagctcttct ctaattcttt ctaaatctcc cagaactgcc
     4141 cggagactga gttcatctcc ccagtttcca aaaccataat accctaagat aaagagttta
     4201 attttctgct gaatgacctc tctctcccca tctccctatt ctctgccaca gaaagagagc
     4261 caattttatc actacaatat agattactgc tacaggaaga ctcagcacca aggcattgag
     4321 aaatcgtaaa aggatataaa gaaaaggggt atgaatatga caaaaagaat tgaaaaaagt
     4381 ggcaaatccc accgacactc ccaaccacag aattaagctg taggcggggc gaactttgtc
     4441 tttatcccag taaaataaaa gccagagggc cgggtaacca atcaaaaatt ctttagtgcg
     4501 gggacgcata aacaaaagac gctctaaaag agagcgaatc ctggtctcta aaccaccggc
     4561 gggcaagagg ggaaagttac ccgagcgagt aaggtaaagt agtgccgcta ttcctaaaaa
     4621 agctactatt aataactcaa atctctttaa gtcctccagt aaaacctgtt gaatgctcac
     4681 cccccagtag ctctttttaa aaagataaaa gaggacaaga agaggaggtg caataagaga
     4741 aacttttact ccccagtact ggtaaattcg caaaaggaac aaccagtggt aaagacccat
     4801 agatacagtt agagccccgg aaaatagagt cataaatgcc catcccaggc tctggagctt
     4861 cccctttcct gaagaaatag cgtcaatcaa aataattact cctgaagtag ggtaaacaat
     4921 accagcaaga agtcccaaaa ccctcatccc ccacagaggg ttatagaaaa ttaatcctgc
     4981 aagaggaata aaacacaggg gaatgatcca gaaagaagga cggaaaaagg aaccccagag
     5041 ataaaaagca aaaaaagata gggccagaga aaagaaaagt aaagaataaa ggggaggaga
     5101 aaaagaagta gaaggaacgc tcacctctcc tggagtatac ccattatcaa ccagctcctt
     5161 tttaactgag agtaagtaag ataagttttt ctcccagctt tcaaaagcag gaggatcggt
     5221 aaaaagacgg agataaagga tgggaactga cctttccttt accgcccgta aaaaccggtc
     5281 ccgggcttct cccggggtat agcccttcag ctcctcagga gggatactgt gagcccgaag
     5341 agtaaaatga ggggcgagag aagcaagggt ggtttctcct ttttgtccca caaactcaag
     5401 atatccccag aacagcccct tttcttccag aaaagaagag agaaaacgca gtgaatccgt
     5461 gcgaggtgct cctagaacgc tatctccaga aaaaataatc ccttccgtca tttcccaggc
     5521 tttttcttcc agcaaacttt ctaaaaccga gggagggata ttataacgat tataaggacg
     5581 gagtataagg cgaaacccct gttcttgtaa agcctgtaaa attctataat cccagcccac
     5641 tcctatttta tcttcttctt catagaggat atttaccccc aatatctctc ctttttcttc
     5701 cacctctttc cctaaaagaa gaagatgggt tttcactgct tctcgcaaaa gaggatcaga
     5761 aatctgaaaa taacgcaaga taggagaaga aaaaggagag ggagctactg gaactgccct
     5821 tccctgactg actaattgcc tccaggtaag ttcactaatt cctaaactat taaatccttc
     5881 ctttttcagt tcttcagcaa gaagtagggg atctactccc tctgtcttcc ccacccattc
     5941 taaatcattc cagtccacca ccagctctac cactctggat tgagattccc gctcccatct
     6001 ctcagaaaga ataggaagag agaaaaagaa gatgattaaa aatcccacca gtaaaaaaat
     6061 attcttcctc atcttccctc ctcaatttct aaaagagggt ctccactatc tacggtgtca
     6121 ttttcttcca catagacctc ttttactatt ccctctcgct ctgcagcaat ctcattttcc
     6181 atcttcatag cttccagaat aattaccacc tgtcctgctt ccactttttc tccttttgct
     6241 acattaatgg ccaaaactct tccgggcata ggagcccgaa gagtggtggt aacctggaga
     6301 ggagaagggg aaggaaccac tttttttata tctcttatat tccagtggct ctctccttct
     6361 tctcttactt ccgctacctc aacttcatat tcttctccat tcactcttac taaaaatctc
     6421 ctcatcgctt acctccccaa gcgaagcatc cagcaacgct catgcttacc cagttctttc
     6481 cactttttgt ttttcttcgg tacatagggc aaagaaggtt tttcttcttc cagaatttca
     6541 aaaagagcag cagcgatagc cgccaccact tggggtttta atttcacacc caaatcatac
     6601 tgggaaatta ccatgtttct ttcccttccc ttcttctcgt ttagaccaga aaacttcgag
     6661 agccttggct actttaatcc gagtttctcg aggatcaatg acttcatcca cgtagcccct
     6721 tcttgccgcc tgataaggat tataaaactc ctgacgatat tgttccagta gtttttccct
     6781 ctctacttca gaatccagcg ctttttctat ttcctgacga aaaatgatgt ctactgcccc
     6841 ctctgcaccc atgacagcaa tttcagcagt aggccaggct aaaactaaat cggcgcccag
     6901 gtctctacta cacatagcta aataagctcc tccgtaagct ttccgtaaaa taaccgttat
     6961 tttaggaaca gtggcttctg aataagcata gagcatttta gccccatggc gaataatccc
     7021 ccccagctcc tgattgctac ccggtaaaaa accaggaaca tcaactaaag ttagaagagg
     7081 aatgttaaaa caatcgcaaa aacggataaa ccgggaagct ttatccgcag cgttaatatc
     7141 cagacaccct gctaaatgat aaggctgatt agccaccacc cccactactt gccctcctat
     7201 ccgggagaac cccactatta tattacgagc gaagtctttt tgcacctcta agaaactgcc
     7261 tctatccact aactcttcca ctacttcttt catatcatag ggcttattgg gatgaggaga
     7321 gactatctcc aaaagagggg tattatgtct gttccagggg tcggaagaat cccctaccgg
     7381 aggatcttct acattgttgg aaggaaggta actcaagaga gtacgtacct ttaaaaagca
     7441 ctcttcttca gtagaagcca caaaatgagc tactccactt tttgtggcat gtgttgttgc
     7501 tcctcctaaa tcttcagccg aaatctcttc gccagtggct gcttttacca cctgaggtcc
     7561 ggtaacgaac attttgctct ctccctctac cataaagatg aaatccatga gcgctggaga
     7621 ataaaccgcc cctccagcac agggccctaa aataagagca atctgaggga taacaccaga
     7681 agcctgggtg ttgcgaaaaa aaatctgtcc ataaccagaa agagaatcta tgccttcctg
     7741 gatacgagca cctcctgaat catttatgcc tataataggc gcacccactt tgagcgccat
     7801 atccataatt ttggtaattt tccgggcgtg catctctcct aaagacccac ccatcacagt
     7861 gaaatcttga gaatagacaa acactaccct tccttctatt gttccaaaac cagttaccac
     7921 tccatcagcg ggaagctttc tttctgccat accaaaccga tcggcccgat gctctataaa
     7981 aagatcgatt tcttcaaaac ttccctcatc caccaacaac tctatcctct cccgagcggt
     8041 gagtttccct gcctcatgtt gctttttcag ggctttttct cctcctcccc gaagaaggag
     8101 agctttctct ttttccaact cttccactaa ctctaataaa gttttcttag acataaacca
     8161 gctgttcctc tctatctgct attccttcta cattgacatt cacagctctc acatccaccc
     8221 cggtcagata ttctaatcct tccttcaatc tcttctgggt taaatgcccc acttgagaaa
     8281 gagagaaatc aggattgatg atacaattca ccactatctc tactcctctt tcggagtagt
     8341 gaatagacag cccttttact tcttctatcc agggaaaacg agaaagaaga aaatcaagaa
     8401 gagaaagaag ggctttctcg gaaatcacta atttacccag ataactaaaa ggaggtcgta
     8461 ccactgtttt ctctcttgaa gaataaaacc acctccagat agcaaccggc acagtatcca
     8521 tcaagctgct ccacaagttc ctcttcaact ccacaagagg cacaggaata gtatgtttac
     8581 cttcgttctc ccgtgccgat agcgccagct ctatctcctg aggagaaact atcttctcaa
     8641 tctctataac ttcctcagga agaggcagct ccagatgctc acagattttc tctaccatac
     8701 gaagagagac tcccaatatc attatatttt gaggagcaag cttcctcaga gcttccttaa
     8761 ccgcttcccg atccggagaa aaatagaaaa gagctatttt agttgcccta attttattaa
     8821 tctccgcttt tgccgagcga ccagcgataa ttctcccccc cgaaataaga agtccgtcat
     8881 caattataca atcaattttg tatttctggg ctaaatgagg aacatgataa ctcttacccg
     8941 ttccgcttgg cccaatcaaa gcataaactt tcatgaatcc tactcctttt cggaagaaat
     9001 ttgactcttc tctaaaaaag cttctacaaa atcatcaatt tctccgttca tcactcgctc
     9061 aacgttgcct gtttcgtgac cggtacgatg atctttcacc aaactataag gatggaaaac
     9121 ataggtgcga atctgattgc cccaggcaat gtctttacgt tctcctttca tctccataat
     9181 ctttttcttc tgttcctctt gataatattc ataaagtcga gactttaata tcctcatagc
     9241 cagagcttta ttctgatgtt gagagcgctc attctggcac tgcaccacta tgccagtagg
     9301 catgtgaata atacgaactg ctgaatcagt tacattcaca tgctggccac cgtgacctcc
     9361 tgcccgaaaa gtctctattt ttaaatcttg aggttcaatt tccactttaa tatcgtcccc
     9421 catctcagga gttacttcta ctaaagcaaa ggtggtatgg cgacgacgat tggcatcaaa
     9481 aggagaaata cgaataaggc gatgcactcc cctttctgat ttgagataac cataggcata
     9541 atctccctgc accaggaaag tcacatattt taagcccgct tcttcccctt ctaaaatatc
     9601 ggtcactcga acttcaaaac cttttctctc acaaaaacgg agataaaggc gaaaaagcat
     9661 ttccacccaa tcttgagact ccactcctcc tgctccggaa tggatggtaa ctatggcatt
     9721 atgactatct tctgtctcag aaaaaagaag ccggatcttt aagtcctctg cttcctgggt
     9781 taagctttta atttcctttt tcagctcgga aaattctgag tccgtttcgc taatcagaga
     9841 actgaattct tgtgcttctc tccatcgctc ttgtaaattc tgatattgct cccagatctg
     9901 gcgataagat ttgtattctc ccagaatctg gttttctcca cctctattat cccagaaacc
     9961 ctcccgggac attttctttt ctatttccct tattttctct tctaaattta actggtcaaa
    10021 gatagccccc tatttcttcc agtttacctt taacttcttc taattcttct attacatcag
    10081 gcatcttttc tcttctcctt tcattcattt ttcccacagc aatatttata ttttaaacca
    10141 cttccgcaag gacaggggtc atttcttccc accttatttt tactccgcgc tgtcgccact
    10201 ttttcccgag gaggtttagg tgaggaagcc ctggatgagg gctttttagc ttcggtaacc
    10261 actcgtagat gtaaaatata gcgaatggta tcttcccgaa tatgagctat catatcctgg
    10321 aacatatcaa aagcttccag ctgatactcg acaaaggggt ctctttgccc tatcgctcgt
    10381 aatccaatcc cttcccggag atggtccata ttacgcagat gatctttcca gtaactatcc
    10441 accacccgca agagcacata tctttctaac tcccggaaca gaggttctcc tatttcctgt
    10501 actttgcttt cataaatttc tcgtaaagac tgcaaaatat cctcttgcaa aagagaagga
    10561 taatagctga gttttttttc ttcgtctact ttccactccc atcctaaaac ggaaaacaag
    10621 cgctgactca gcccttccca atcccattct tcaggataaa ctttttctgg agcataagca
    10681 gaaactatat ctttaaccac ttcttccatc attcccagaa tgtgaggacg aagattttct
    10741 tctcgaagca ccattcttct ttgctcataa attacccctc tttgtttatt catcacatca
    10801 tcatactgaa ggagagtctt tctaatctgg aaataatgcc cttctactct tttttgagcc
    10861 gattctatag ctcgggtaac caaagaatgc tccagaggaa ctccctcctc cattcccaat
    10921 ttttccatga gttgagatat tttatctgaa ccaaaaaggc gcaacaggtc atcttctaaa
    10981 gagaggaaaa attgcgatga gccagggtca ccctgccttc ctgcccgtcc tcgtaattga
    11041 ttgtcaattc tcctgctttc atgtcgctct gtaccaataa catgcaaacc accagcagct
    11101 ttaacttctt ccccttctcg gtcggttatt tctttaaacc gggaaagaag ctctcggtaa
    11161 acctctcgag cttcttttac ctctggtaag tcagtaggta agccttctgc ggcctcgaga
    11221 attatttctt ctttaaaccc cttacttctc atttcttctc gagccagaaa atcaggatta
    11281 cctcccagaa gaatatctgt tcctcgtcca gccatattag tagaaatagt aaccgctcct
    11341 tttttcccgg cctgagcaac aatttccgct tctttttcat gatgtttagc attaagcacc
    11401 tggtggggaa ttttccgctg gtgaagaaga ttactcagcc tttctgactt ttcaatggaa
    11461 accgtcccca ctaaaaccgg tctccctgct ttattctgtt tttcaatttc ttcgactact
    11521 gcagcaaact tctcctcttc cctgcggtaa ataacatcag ggtgattggt tctgtggaga
    11581 ggtcgattag gaggaattac cactaccggc ataccataag tgtaaataaa ctcactttct
    11641 tcagttttag ctgttcccgt catcccgctg gttttgttat acattcgaaa ataattctga
    11701 taagtaatgg aagctaaagt ctgattttca ttagccactt ttaggccttc tttggcttct
    11761 atagcttgat gaagaccatc gctatagcgg cgtccatgca tgagccgacc agtgaactca
    11821 tctacaatga ttacttctcc atctttcact acataatcga cgtctttttt ataaagatga
    11881 tgagcccgta aagcttgcct caccaggtgt tctaaagaac ctttcccggt ttcctcataa
    11941 agattatcga tatgaagcaa gttttccact ttggtgactc ctttttccgt caaccatatg
    12001 gaacgggtct tctcttcata atcgaagtcc tcaggatagg atagcctcct caccacttta
    12061 ttcactttgt aataaatttc tgtgtcttcc tctgctggcc cagaaatgat taaaggagta
    12121 cgcgcctcat caatcaaaat actatccact tcatcaacaa tagcgtaatt taactctctt
    12181 tgcactacat cttcgaagcg gataaccata ttatctcgca ggtaatcaaa accaaactcg
    12241 gtattagtcc cgtaggtgat atcacagaga taagcctttt tacgctccgc aggagaggag
    12301 tcatgctgaa tcaaacccac cgataacccc aaaaactcaa aaataggccc catccagtaa
    12361 cgatctcttt tggccagata atcattaaca gtaacgatat gcactccttt tccttctaaa
    12421 gcattgagat aagcaggcat ggtagctacc agggttttcc cttctccggt agccatctca
    12481 gcaatttttc cctgatgaag caccacgcct cccatgattt gtacatcaaa aggacgcata
    12541 ccggttgttc tctttgctac ctctcgaact acggcaaaag cctccaccaa taaatcatct
    12601 aagctgcttc cttcctgaat tcttctttgg aattcttcag ttttgtgccg taattctgca
    12661 tccgagagac gggaaacctc tttctcctga gcgttaaccg cctccacata tttatctttc
    12721 aactctcgaa tttgcttagc ttgagctcta tcagcccaaa aatctttaat ccagcccatt
    12781 attttcctcc tgaatacacc cctaaattat aacacaacca taacccttat aaacccggca
    12841 ataaggggaa aaccttccct ataactccct tttgcattcg ccaccccctc aaattcgtca
    12901 ttcttgaata aatgtttccc ctttatgaac tcccttcttt ttgccgtcac cccttgagtg
    12961 tttctatcac ctttactcct gaatggttct gcgaggggtc cacagtattt aaaagcgttt
    13021 ggattcccga taaaaaattt caggaatgac ggaaagaggc gtcattcctg aatgtcccca
    13081 tcagaggtcc aagttataag gggaagaaaa actctccctt ataactcccc aaaagactaa
    13141 aacaaaaata cagaactact tacggtgtaa gattaaagaa aagagattaa aagcgaaaaa
    13201 gattaaaaat tacgctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac
    13321 atttagatga tatcgcctca acctgataga taattattcc tttttgtcaa tttagttcac
    13381 atcaaaagct cctccggcct agagcagata gatatgggta gcaataaagc ccgaaaagtg
    13441 gaaggaaaat acaggagagg acaatgtgcc atcctctcca aggctttcgg tattcggatg
    13501 tctgatgaaa aactggaggt ttggctctcc aaacctccag agagaaattc ctttataatc
    13561 gcgaagaaga agcataaaaa aaactggagg gggaccctcc tctaacaaac taaaaatctg
    13621 agagtgctct ctcatctccc ctctccgggg acttttcctt cctcagtgtt ctcgtagtca
    13681 actattggct atcgtctcga gttgagaaac gagagctatc cagtgttcta tttccaggtg
    13741 ttctgcccgc cactcaggat gaatatgagc ccgagctaaa atttctctgg ttttctctct
    13801 tcccaattga ggacggagaa gtttttctac tttcttccgc cgctgagaaa atagaaaatg
    13861 aacccagcgc ataatttcct tataagcatc aggctctatc tggggagaaa gaggaataaa
    13921 tttaagaatt agagaacgaa ccgcaggggg aggagaaaaa gaaccaggag aaacctctct
    13981 tactttttct acttcataaa ggagattcac tcccactgca agagggcttc cttttttctg
    14041 caacactttt tctccaaact cccgctgaac cattaaaact agaagatccc acttaattcc
    14101 actttccaaa attttagaaa aaaagggaga agagatagca taaggaatgt tagaaactaa
    14161 cttgagggaa gaaaaagaag aaaggagaga cgctaaatca gctgataaaa aatcttctct
    14221 ataaagaact aaattagaat aatcttttaa cttttcccga accatccggt aaagccgctc
    14281 atcaatttcc caccctatta ctttaccggc tttttgcacc aaaacttcag taaggatccc
    14341 cttccctact cccacctcta ctactgtgtc ttcctttttc aggtcggctt tatcgattat
    14401 tatatctcgg acagccttat caaccagaaa atgctgcccc catcggggag agatatggaa
    14461 gctggtcaaa attagcccct taaatcaccg aaactaccag actaagggtt taacatggaa
    14521 tagttctcct ccgggtgttc caggtgtcca gggaaaaaat aatcacagcg gtctactggc
    14581 tcttgtcttt ctttttgctc tcctaataga cacttataaa tccccttttt cttcatttct
    14641 gcgtcgcaat gcctgcacac ctggcataaa ccttcaggat tgtacaattc aaatcacccc
    14701 tttttttatt ttattctaaa aaccattcca cttcagaaac ctctacattc accccctcca
    14761 gagggaatcc ccattctctt atcatattag ccactcgctt ctgcacctct tgcgctatct
    14821 cttcccagtt tttccgagaa tccactttta aggaaatatt aataaatacc cctttttctt
    14881 tagtcgagac tctatcattg ggggagagag tttctccttg cacaacgacc ttggtattcc
    14941 ttaagagatg gttgaaatcc cccattaccc cctctaccag gaaagcgctc cgcgccgcca
    15001 tttctaccag ggtagacaca ctcactctaa tttctcctgg ctcttcacta aagggcatta
    15061 ttactctcca gtttctccca aacataatgt agtgcttcct tagcatcctg gaattgagga
    15121 aaaaaatctt ctttgcccgt agaatcctga attttccagg tattcaatcc ggcgagaatt
    15181 ttgtcatgag aaagagctaa ggcaatttca cttaaagttc cagattgacc cccacaggct
    15241 atgagagccg aagcagttag gacaattatc aggttacggg cttctcccag accggtcaca
    15301 atttttaaat ccacataagg gttggcatct tccctggaat tgccgggcaa aatacctaca
    15361 gtgaaaccgc cagcttcttt tgctcctcga caagccgctt ccatcacccc tcctaatcct
    15421 ccgcaaagga gaatagcacc tcgttcggca atcccttttc ctactcgata agcactttca
    15481 tatagttctg cactgcagga atgttgacca ataactccta ttatccagtt atgattcata
    15541 acccagatat ttttttaaat cttccggttt taaattatct aaaaactgac gaaactcctc
    15601 tatttcctta tcgtctatct ctcctgttgc tacagtagta gagctgagaa cctcctcgga
    15661 agcaaagatg ggcgccccca ttcgtaaaga aagtgctatt ccatcgctgg gacgagcatc
    15721 tatggtaatt tcatttccct ccacctgaat atatagaaga gcataaaaag tgttatccac
    15781 aattctagta atgactattt tttccagttc tcctcctaat tggtggataa gagaagccat
    15841 gaggtcatgg gtaagagggc gaggagtctt aactccctgt aaacccagaa gaatggcctg
    15901 cgcctcaaac aagccaatcc agataggaag gctccttttt tcttcttcat cgctcagaac
    15961 taccactgcc atagtgtttt tctggtcgaa catcaaaccc tgaactttca ttttgatcat
    16021 aactaaaaac cctttctatc ttacgtttta ttattatagc gtaaatcaag ttttcttcca
    16081 aaaatcgagc aaaaattctc ccatactttc tccctcaaag tcaaaagaga aacaccccat
    16141 aactgagcca gaaatgacgc agtctcaact aaaaatgctg gctcatttct cctgcctctc
    16201 cgcgattgag gaggaaggta aggagcatcg gtttctaaaa gaacacgaga aagggggatt
    16261 ttttgaacta cttctcgcag atgggtagcc ccggggtagg tcaccactcc ccccagggaa
    16321 aagtaaactg ctcgaggtag tgcttcctgt aaaatctcct cgtcccccga aaaacaatgc
    16381 cagattacca ccacatcccg caccttctct tttataatct gccataaatc tttattagat
    16441 tcccgggagt ggacaattaa aggaagagaa aacttttctg ctacttcaat ttgctggata
    16501 aaagcttctt tttgtttttc gatatgactt ctatcccagt aataatctaa tcctacctct
    16561 cctatagcta taaccttttc ctttcgtaca tattcctcga ggatagagaa gtcgggcaat
    16621 tcatccccta tctcctgggg atgaatgccc acactagcat aaagcgaagg ataacgctcc
    16681 actaacttta aagtctcttg aatcgaggag agagaagtag cagcattgac tataatctct
    16741 actccagcgt tttgtgctct gtttataacc tcttctcgat cttttagaaa ttcctcccct
    16801 tctaaatggg catgaatatc tacccaaaaa ctcatcttac tttactccct ggcgctatat
    16861 ctcgatccac agtcaagatg ctaactgctc cttttccatc atcagcagct aaaagcatcc
    16921 cttctgattt gatcccccgg atattagccg gttttaaatt agtgagaaca atgatttttt
    16981 ttcccatcaa ctcctcaggt gtgtaataat tagccaagcc cgccaccagg gttctttctt
    17041 cttctcccag gttgactcgc aaagccaaaa gagctttagt tccttccact ctttctgctt
    17101 ttactacctc tcccacccgc aggtcaatat tttgaaaatc ttccagattg atttccggca
    17161 ataccatttt tttcactcct ctactctatg attctcggga acaaaggctg agcctcctcc
    17221 actgaataaa cttccggtcc tccttgccag cacagacttt cttccaggct ttcttttccc
    17281 ggatcctgct gcaaacctaa aagctctctc aacttcaaag tagtatcagg cataaaaggg
    17341 taaaggaaaa aactcagtaa ccgagcattg tctaataggc gataaagaag attttccacc
    17401 tctttttctt tcccttcttt gtgcaatttc cagggagctt ctcgctcgat gtagcggtta
    17461 gcaaaatg
//
LOCUS       DAOPSS010000023        17107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2808_length_17107_cov_8.707718, whole genome shotgun sequence.
ACCESSION   DAOPSS010000023 DAOPSS010000000
VERSION     DAOPSS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2808_length_17107_cov_8.707718"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..357
                     /locus_tag="PKN31_02665"
     tRNA            282..357
                     /locus_tag="PKN31_02665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:315..317,aa:Gly,seq:tcc)
     gene            453..701
                     /locus_tag="PKN31_02670"
     CDS             453..701
                     /locus_tag="PKN31_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98866.1"
                     /translation="MADLLLIKVGNRAQNALKVQELLSRYGCNIEVRLGLHEFAPECE
                     GKDEGIIILKLSGNLEDFKKLKEDLEKIEGVKAIYQEL"
     gene            741..3221
                     /locus_tag="PKN31_02675"
     CDS             741..3221
                     /locus_tag="PKN31_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA98867.1"
                     /translation="MESNVDLSQYRLSPEQLRWICPPDAFSFECTDELEELEGFLGQE
                     RALSSIEFGLRMEKSGYNLFVVGPTGTGRASAVKNRIERLVKEKKEKGITPEINDWCY
                     IFNFDDPDRPQVLKLPRGEGKVLSRLVDELLKNLRDAIPKTFAGDEYKNQKQILIDEQ
                     QRKNRQIIQELEKEALEEGFAFRMTPMGPILIPLVEGKPMTQEQYLALGEEEKEIIES
                     KRRELLTQISEAFDKIHQLEVELKNKIAELDRRVAEFAVNPLFKGLLDRYASSPEVLK
                     FLESLQNFTLSYLHIFRDLSETPQQLPQLLPYQRLQDPFLPFKINVFVDNAPLEDPPI
                     ILETHPNWSNLFGKIERRAFMGAYFSDHTMLKSGSLHQANGGYIIMYFRDLISNPGVW
                     EGLKRTLKNQEIRLEDPFEQFGIIAPQGLRPDPLPFDAKVIIIGDEYYYRALTLLDED
                     FRDLFKIKADFDYQIQREEEVLHSFACFVNNCCKEEGLLPFDRTGVAKVLEYGARAVS
                     HQKKLSSQFGFMKDILVEADFWAREEGADRVYAPHVQKTLQERKKRLSLIADRLQEAI
                     LEDVLLIDTQGKVVGQINGLAVYDLGDYSFGRPSRITARTYLGKQGVVSIERESRLSG
                     RIHDKGVLILGGYLGYKYAQNKPLSVAASICFEQSYEGVEGDSASLAETCAVLSSLAE
                     VPIRQDIAVTGSINQKGEVQAIGGVNQKIEGFFRTAQGKGLTKEQGVIIPRSNIQNLM
                     LEEEVVEAVREGKFHIYAVSAVDQAMEILTGLPAGEKKPDGTFEEGTLNYLVDEKLAK
                     TVELLRSFEAKEKEEEKREENTSENKEG"
     gene            3225..3737
                     /locus_tag="PKN31_02680"
     CDS             3225..3737
                     /locus_tag="PKN31_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOA98868.1"
                     /translation="MEEISHQGLRWLVRTALLFALTLVIQMLGLPQFFTGPLVNAFLL
                     LAALLNGIWSGVIIGLFTPWIAFARGILAPPLGPMIPFIMAGNAILVIVFSLVAGKRE
                     NLRREITGIILGSFLKYLLLSQAVAWVVQVPPPVAKAMQTPQLFTALAGGAIALIVYR
                     VIPARFKSYK"
     gene            complement(3774..4127)
                     /locus_tag="PKN31_02685"
     CDS             complement(3774..4127)
                     /locus_tag="PKN31_02685"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOA98869.1"
                     /translation="MSEEKTARPSKTKDLGFYFLGLLSAKAWQYLGLVVHPETGEMLV
                     DLEEARKSIDLYSVILEEVKKDLEGSELRELEAQLSQLQLNFVEKSKETKKPEETKKP
                     EEAEESKEAEESEES"
     gene            complement(4124..5062)
                     /gene="miaA"
                     /locus_tag="PKN31_02690"
     CDS             complement(4124..5062)
                     /gene="miaA"
                     /locus_tag="PKN31_02690"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOA98870.1"
                     /translation="MVQLKGKKLPAIVGPTATGKTDLSLALAERLGKVELIYADSLAV
                     YKYLDIGTDKPPASFREIVPHHLVDFLDPGERWSAFQFKKEAIYLQREIRERGNLPLV
                     VGGTAFYLQALYRSFLFVGAPAENNIRIAFERLPTEKLYALLQNVDPRRAQKIGKHDR
                     KRLIRALEIFEKTGRPPSLFSPRPSSLSPLLIGIRWDRDKLKERICRRVEEMFERGIV
                     EEVEELFSRGYSEDIPALDNFTYRPIVKLLRGDISRKEAQEEIVRGTFRFLKTQINWF
                     KKAPVIWIEGEGKPLSILVEETCAIIKRNLVEEDVK"
     gene            complement(5043..6413)
                     /gene="miaB"
                     /locus_tag="PKN31_02695"
     CDS             complement(5043..6413)
                     /gene="miaB"
                     /locus_tag="PKN31_02695"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOA98871.1"
                     /translation="MNLVEIKRKPDFPAGKVAEATFWVETFGCQMNKSRSEHLSYRLQ
                     ELGFSPAEESGEADILVFNTCAVREHAAERAISIISKFARERKEKNPDSIIVVCGCLG
                     QLEREKLLGKDREINVLAGTHNWEKIPLLIKKAWQEKVKIVDFQEEPPSLYEEKGYRY
                     SSKISAYLPITFGCDNFCSYCVVPYTTGKQRSKPREIIEEELKNLVEEGFREVTLLGQ
                     NVNSYGRDREREGAFEELLLDIEEWLRGIKIWVRFLTSHPRDFTEDIVKIVKDSSVLC
                     PYFHLPLQAGSNKILQLMRRGYTREDYLNKINFIRKSIPSARIGTDIIVGFPGETDSD
                     FRETLDVVREAQFDVAYTYVYSPRPGTAAYLLEDDIPVCEKKERLHILNDTLRSIYQE
                     KLPSLQGKVMEILIDQEEGGLLSGRGANNLRVYLKELSSAEVGEWREVVLTPGEGNKV
                     YGSVKR"
     gene            complement(6424..6690)
                     /locus_tag="PKN31_02700"
     CDS             complement(6424..6690)
                     /locus_tag="PKN31_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOA98872.1"
                     /translation="MDVLKVSSKSNPNAVAGALAGAIREKGIVEVQAVGAGAVNQAIK
                     AIAIARGYVAPSGIDLVCIPAFTDINIDGEERTAIKLVVKPRSA"
     gene            complement(6721..7494)
                     /locus_tag="PKN31_02705"
     CDS             complement(6721..7494)
                     /locus_tag="PKN31_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOA98873.1"
                     /translation="MRFLIVGDIVGKPGRQVLADNLEVIKEKLNIEGVIVNGENAAGG
                     LGIVPEVCEEILSYGVDVITTGNHIWDKKEIVDYIDYQPRLIRPLNYPSGVPGRGWVA
                     VEGKGKKWIVANMSGRVFMPSLDCPFRRIEEELEDFRRITNMILLDFHAEASSEKIAM
                     GWFLDGKVSCVFGTHTHVATADERILPQGTAYITDIGMTGAHDSVLGVKTEDILSRFL
                     TQMPIRYRVAKDNVKLNGIVVEVDEITGRATEISRLSIS"
     gene            complement(7510..9039)
                     /gene="rny"
                     /locus_tag="PKN31_02710"
     CDS             complement(7510..9039)
                     /gene="rny"
                     /locus_tag="PKN31_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOA98874.1"
                     /translation="MELIYILIGIAVGLVAGFSFLNSQLQKERKIAQEEIERVREETL
                     GQVETLRKEALILAKEEVLEERKSLEEEIQRKRKELQNFESRILRREEMLERKLEQVE
                     KTENLLRRRQEELEKEKEVVESIKAREVEELSRIANLSWEEARQELLERVEKTLDYEV
                     GVKIKEAEERAKREAGKMAQEVVAQTIQRYAADFTVENTVSVVNLPSDEMKGRIIGRE
                     GRNIRTFEMITGVDLIIDDTPEAVLISSFDPIRREVARLALEKLIVDGRIHPARIEEL
                     VDKAKLQVEEKIIQEGEQALFDAGVKNVNSELVKLLGKLYFRTSYGQNVLQHSKEVAY
                     FASLMASELGVDTTLAKRAGLLHDIGKALDHEVEGPHAKIGAELASRYGEKKEVVHAI
                     EAHHGEVEAQTVEAVLVQAADAISASRPGARRESLESYIKRLEKLEKIADGFEGVEKS
                     YAIQAGREIRIMVKPDRIDDARAAKLAYDIARKIEKELEYPGQIKITVIRETRTVEYA
                     K"
     gene            complement(9122..9553)
                     /locus_tag="PKN31_02715"
     CDS             complement(9122..9553)
                     /locus_tag="PKN31_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOA98875.1"
                     /translation="MKDIIRQGFRYLNRKMYTRGELFQKLARDGFSREEIEEALQFLE
                     ERGYLNDEEFVKIYVETRRRLNPRGLQAIKDELRRKGVSHELLDSLREFFPREEEVKD
                     ARGLIREWSGEGKEKEDINNRLLRRGFSWEVVEEAWYREES"
     gene            complement(9550..10581)
                     /gene="recA"
                     /locus_tag="PKN31_02720"
     CDS             complement(9550..10581)
                     /gene="recA"
                     /locus_tag="PKN31_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOA98876.1"
                     /translation="MSEEKSSKDLREDVINQAISSIEKKYGKGAIMYLSQDVLTADIE
                     VIKTGSVLLNIALGVGGFPRGRVVEIFGPEASGKTTIALHAIAEAQKQGGIAAFVDTE
                     HALDPSYASKIGVQIDRLLISQPGSAEEALDIVDALVRTGGVDIVVLDSVAALVPQME
                     LEGGMSDSYIGLQARLMSQALRRLTAYISKTKTVAIFVNQLREKIAMAYGPSETTPGG
                     RALKFYASVRLDVRRREYLRSGDEIIGALTRIKVVKNKVASPFKEAELELIYGEGISQ
                     EGEILSLGETTGVVKKSGSWFSFGEYRLGQGRDNARQFLKENPEVAKEILSAALSQME
                     VDPVIAFNS"
     gene            complement(10574..11149)
                     /gene="thpR"
                     /locus_tag="PKN31_02725"
     CDS             complement(10574..11149)
                     /gene="thpR"
                     /locus_tag="PKN31_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOA98877.1"
                     /translation="MSSTTIRSFVALDLPSEAQDFLEELINEEKKLYPQAKWVKREQL
                     HLTLHFFHAFPLGEVEEMNKILEEVSNKFTAFPVTLEKWGAFPSLHRARVVWIGVDRE
                     GEGKIKSIREEVEKGIAAKGWEREDREFSPHITLARFRSPFALPSLGVENINFSTEIK
                     KLVFYQSTLTPRGSVYQQLGVFSLEGVKKNE"
     gene            complement(11100..12395)
                     /locus_tag="PKN31_02730"
     CDS             complement(11100..12395)
                     /locus_tag="PKN31_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOA98878.1"
                     /translation="MKAAILGIGTELLTGLVRDYNAYFLAQKLVDKGILPQFILFVPD
                     QMEKIVDALQFIMKEEEVKLVIVSGGLGPTEDDLTREAVAQAFGRSLIFSPEAWDRIQ
                     FFYRELRNTEPPENNRRQAFIPQGAEILYNERGTAPGFKLEVNGKSIFVIPGVPREAE
                     FFWSLIEKDLPREEEYFYRSPIFKFCGIGESNLAKEVEPFLRHLPSSFNLAFLPNYGE
                     VWLYVYSSRFSPEEKESALIFLEEVARHLDRFFFSPYGDTLEEAVGNMMKEKGLKLGL
                     AESCTGGLVANRITDIAGSSQYFERGYVVYSNEAKKEDLKVPEEILQEKGAVSEETAR
                     FLARGVRHKGKVDLGLGITGIAGPGGGSKEKPVGLVYIALSSEEGVRVQKFHFSGDRL
                     MNKRFASQSALVMIFQYLKEKFGNEFNYHQKLCGFRPSL"
     gene            complement(12392..13723)
                     /gene="rimO"
                     /locus_tag="PKN31_02735"
     CDS             complement(12392..13723)
                     /gene="rimO"
                     /locus_tag="PKN31_02735"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOA98879.1"
                     /translation="MRKIAFISLGCDKNLVDTEVALGKLLSRGYNFFSSPEEAEILIL
                     NTCAFIEEACRETEAWIEKLSFLKEAHPPKKLLVMGCYAQRWGRKKGFVYPAVDYWVG
                     VNDFPSLVDILEDKFPGKYFLDSPPFLYHHETERILSTPSHYAYLKIAEGCNHHCSFC
                     LIPSIRGSLRSRTMESIEKEALNLVSQGVKEIILVAQDLSSYGLDLYHQRMLSPLLRR
                     LDEILPSGVWLRLLYFSPEGLDDEFIKTMRDSSHLVKYLDIPLQHVDSTILQKMRRPS
                     SIEKIREKLEKLRECVPGIFLRTTFLVGFPGEDEEAFQRLVDFVEDFRFERMGAFVYS
                     EPPDTPSAFLYPKVEEKVKQERYKALMEVQEKVMGKFHSSLVGRELEVIVDEAQRGNG
                     VTNFWGRTYGDAPEVDGNISFSSRRNKLKRGEIVKVKVTESSSYELKGEKI"
     gene            complement(13720..14469)
                     /locus_tag="PKN31_02740"
     CDS             complement(13720..14469)
                     /locus_tag="PKN31_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOA98880.1"
                     /translation="MAEGQLKLGEILREKREERELTRDEVSGVTHVSSRYIAALEEED
                     WDKLPGRAYALGYLKIYSRFLGLNEEEMVSLFNQVYSVKPVDWKSLDWKKTEEPELRS
                     ARDRKSDRSTLFKRLLVLLVSLAVFFTFLFIFFSFHSLPEGEEVVVNPPVSAIEPEEI
                     LPSPSAEVGPQFSLTVTLFPEKAAWAEIWSGGNEVFSGILVPNKEYVVKSNYPIEVEI
                     KGGNEVKAVVNGKDWGYLSEKEEASRQVFEP"
     gene            complement(14471..16561)
                     /locus_tag="PKN31_02745"
     CDS             complement(14471..16561)
                     /locus_tag="PKN31_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOA98881.1"
                     /translation="MNKKLLVVCFLSFFAIYSLLIVFSFDVGELGELIGFYLFRFLGV
                     GAYLLPFFLGYLAYEIYHFQPARKFRVTSRIISFSLWIVIFLTLSQRAAERAGFSFSD
                     GDFAGIVGAFLYRHLVYYLGDTGTILALFFTAFLAVYLGGEGKLLRQLSQGWRFLKRK
                     GGELFSRRREIPQREIASSGVSRPIKVKEKEITSSSWEGEALDEVFAPLELKAKEEAQ
                     AKKKLTSSSRKKKSTTWTLPSPQILRVYPSRGGEKTKREIIGEIDKLEQTLAEFNVLG
                     KVVNVQVGPTITRFDFQPAPGIKVQKITSLSNDLALAFAVAAVRIEAPIPGRSAVGIE
                     VPNRHKELVSLRDLIESEEFKKSESPLTVALGRDVSGRPLVWDLREAPHLLIAGATGS
                     GKSVCINSILTSLLYRATPNDLRIGLVDPKRVELSLYSGLPHLCAPVISEARWVVRFL
                     KWLCREMDNRYELLSEVSARNIEEYNQMVEEGEKLPYVVIVIDELADLMMTAPTEVES
                     SLCRIAQIARAVGMHLVVATQRPSVDVITGLIKANFPSRLAFAVSSQVDSRTILDSPG
                     AEKLLGNGDLLFAPLGASKPIRGQGAFVSTSETKKLVECWLNQSGDIYYPLEYVPREE
                     RGVGEEEEDELYQEAVRVVIESGKASTSLLQRRLRVGFNRAARLIEQMEREGIVGPYE
                     GSKPRKVITPKGEG"
     gene            complement(16585..>17107)
                     /locus_tag="PKN31_02750"
     CDS             complement(16585..>17107)
                     /locus_tag="PKN31_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOA98882.1"
                     /translation="RPRSSVSPYAPRIEILEVDPDRIGALIGPGGKVIKKIVEESGAT
                     IDIKDDGKVIVFSRTEEGMNKAINMIKNITQDVEVGQVYLGRVTRVTNFGAFIEIFPG
                     REGLCHISQFSSERGKKVEDFVKVGDDVLVKVTGIDSMGRIALSHKEALGDSDNSEKS
                     NRSKEPRHRSRER"
BASE COUNT         4742 a         4232 c         3198 g         4935 t
ORIGIN      
        1 atacccgtag gggtaaactg gatccctata aagaaaccat agataagtgg cttaccgaag
       61 acctaaaatc ccgaccccag aaaggcatac tacgaaatgt atttaaatat caaagtcaaa
      121 aaatatcagg ctgtttttat caaccaggcc tctctatctt ccagtttttc tcagactaat
      181 caatcaaaaa ttccttctaa tatttgattc aactcctcac ctttcctccc ccttgacgaa
      241 gtctattcca ttatctataa tcattgtgct aacagtagcg agcgggaata gctcagatgg
      301 tagagcgcca gccttccaag ctggatgtcg cgggttcgag tcccgtttcc cgctccattt
      361 ttttatttaa agaacttttt ccagaaagtg taaaagagca aaaaatattc tataataaga
      421 aaaaaataag gcttccaggg agggataaga gtatggctga tttacttctc attaaagtgg
      481 gaaacagagc acaaaatgct ttgaaagtgc aagaattact cagccgttac gggtgcaata
      541 ttgaagttcg cttaggactc catgagtttg ctccagaatg tgaggggaaa gatgaaggca
      601 tcataatctt aaagctttcc ggtaatttag aagattttaa aaagcttaaa gaggacctgg
      661 aaaaaataga aggagttaag gctatttatc aagaattata acgagggttt ttcttttatg
      721 gaaaaacgaa gggaggacct atggagagca atgttgacct ttctcaatat cggttaagcc
      781 cggagcagtt gcgctggatt tgtcctccgg atgctttttc ttttgagtgt actgacgaat
      841 tagaagagct ggaagggttt ttgggtcagg agagagccct gagctctatc gagtttggat
      901 tgcgcatgga aaaatcgggg tacaatcttt ttgtggttgg tcctacagga accggaagag
      961 ccagtgcagt taaaaaccgc attgagagat tggtgaaaga aaagaaggaa aagggaatca
     1021 ctccagagat aaatgactgg tgttatatct ttaactttga cgatccagac cgccctcaag
     1081 ttttaaaact tccccggggg gagggaaaag tcctttctcg cctggtggat gagttgttga
     1141 aaaaccttcg agatgcaata cccaaaactt ttgctggaga tgagtataaa aaccaaaaac
     1201 aaattctgat tgatgagcag cagagaaaaa atcgccaaat aattcaagag ttggaaaaag
     1261 aagctttaga agaagggttt gctttccgca tgactcccat gggtcctatc ctcattccct
     1321 tagtggaagg aaagcctatg actcaagagc aatacttagc tttaggcgaa gaagaaaaag
     1381 aaattataga aagtaaaagg cgagagcttt tgactcaaat cagtgaggct tttgacaaaa
     1441 ttcaccagtt ggaagtagag ctgaaaaata aaatagcgga acttgaccgg agagtggcag
     1501 agtttgccgt taatcctctt tttaagggac tattagacag gtatgcttcc tctcccgagg
     1561 tgctcaagtt tcttgagtca ctgcaaaact ttaccctttc ttatcttcac atcttcagag
     1621 acttatcaga aactcctcag caacttcctc agcttctccc ctatcaaagg ctgcaagatc
     1681 ctttcttacc ttttaaaata aatgtttttg tggacaacgc tcctttagaa gaccctccaa
     1741 taattctgga aacccatcct aactggagca atcttttcgg gaagatcgag cgccgagctt
     1801 ttatgggagc ttatttcagt gaccatacta tgttgaaatc aggctccctt catcaagcca
     1861 atggtggtta tataataatg tacttccggg atttaatcag caatccaggc gtctgggaag
     1921 gactgaaaag gactttgaaa aatcaggaaa tcagattaga agaccctttt gagcaatttg
     1981 gcatcatcgc tcctcaagga ttgcgtcctg accccttacc tttcgacgct aaagttatta
     2041 tcatcggcga tgaatattat tatcgtgctt taactcttct cgatgaggac ttccgagatc
     2101 tatttaaaat aaaagctgat tttgactatc agattcagag agaagaagag gttttgcact
     2161 cctttgcctg ttttgtcaat aattgttgca aagaggaagg gcttctgcct tttgaccgga
     2221 cgggagtagc aaaagttctg gaatatggtg cgcgggcagt ttctcaccag aagaaactca
     2281 gttctcaatt tggttttatg aaagatatcc tggtggaagc agatttctgg gcacgggagg
     2341 aaggcgcaga ccgggtctat gctccccatg tccagaaaac gctacaagag agaaaaaaga
     2401 ggctgagcct tatagctgat cgcctgcaag aagctatttt agaagatgtg ctgttgattg
     2461 atacccaggg taaagtggtg gggcaaatca acgggttagc agtttatgac ctgggagact
     2521 atagttttgg tcggccctcc cgcattactg ctcgcactta ccttggaaaa caaggagtag
     2581 tcagcatcga gcgagaatct cgcttaagcg gccgcatcca tgataaagga gttcttattc
     2641 tgggaggata cttaggctat aagtacgccc aaaataaacc actttctgtg gctgccagca
     2701 tttgttttga gcagtcttat gagggagtag aaggagacag tgcttccctg gctgaaacct
     2761 gcgcagtgct ttccagtctg gcagaagtac ctattcgcca ggacatcgcc gttactgggt
     2821 ctattaatca aaaaggagaa gtgcaggcca tcggaggagt aaatcaaaaa atcgaaggat
     2881 ttttccgcac cgctcaagga aaagggttaa ctaaagaaca gggagtaatt attccccgga
     2941 gtaacattca aaaccttatg ttagaagaag aagtggtaga ggcagtgcga gaaggtaaat
     3001 tccacattta tgccgtctct gctgtagacc aggccatgga gatattaact ggtcttccag
     3061 ctggagaaaa aaagcctgat ggaacttttg aagaaggaac actaaattac ttagtggatg
     3121 aaaaacttgc taaaaccgtg gaactattga ggagttttga agcaaaagag aaagaagaag
     3181 agaaaagaga ggaaaatacc tcagagaata aggagggatg aactatggaa gaaatttctc
     3241 accaggggct tcggtggtta gtcaggactg ctcttctttt tgcccttact ctggtcattc
     3301 agatgctggg gctacctcag tttttcaccg gccccttagt aaatgccttt cttcttttgg
     3361 ctgctctttt aaatggaata tggagcgggg taataatagg gttatttacc ccctggatag
     3421 cttttgcccg gggaattctt gcccccccac tggggccgat gattcccttc atcatggccg
     3481 gcaatgccat actggtaata gttttcagcc ttgtggcagg aaaaagagaa aacttgcgga
     3541 gagaaataac aggaattatt ctgggctctt tcttaaaata tctccttctc tctcaagcgg
     3601 tagcctgggt ggtgcaagtg cctccaccgg tagccaaagc catgcagact cctcagcttt
     3661 ttacagcttt agcaggggga gccatagcct taatagttta ccgggtaatc cccgccagat
     3721 ttaaaagtta taagtaaagg agagaggttt ctcttcttgc gagagagaat aatttatgac
     3781 tcctcactct cttcagcttc tttactctct tccgcttctt caggtttttt agtttcctcg
     3841 ggttttttag tttctttaga tttttctaca aagttgagct ggagctgaga aagctgagct
     3901 tccagttctc gtagctcgct tccttctaaa tcttttttga cttcctccag aatcacggaa
     3961 tagaggtcaa tagatttacg tgcttcttct aaatctacca gcatctctcc agtctcaggg
     4021 tgaactacca accccagata ttgccaggcc ttagctgaaa gaaggcctaa aaagtaaaat
     4081 cccagatcct tagttttact gggtcttgcg gttttttctt cgctcatttt acatcctcct
     4141 ccactaaatt tctttttata attgcacaag tttcttctac cagaatggat agaggttttc
     4201 cctctccctc tatccagatt acaggggctt ttttgaacca gttaatttga gttttcaaaa
     4261 atcgaaaagt gccccgcact atttcttctt gagcttcttt ccgggaaata tcccctctaa
     4321 gtagttttac tatcgggcga taagtaaagt tatcgagagc aggaatatcc tccgaatatc
     4381 cccgagaaaa aagctcctcc acttcttcta ctattcctct ttcgaacatc tcttctaccc
     4441 ggcggcaaat tctttctttt aatttatccc tgtcccagcg aataccgata agaagaggag
     4501 aaagagaaga gggacgggga gaaaaaagag aagggggacg accggttttc tcaaatatct
     4561 caagagctct gatcaatctt tttcggtcat gctttcctat tttttgcgct ctccgggggt
     4621 ccacattttg caaaagggca tagagttttt cagtaggtaa acgctcgaaa gcaatgcgga
     4681 tattattttc agcaggagct cccacaaata gaaaagaccg ataaagcgcc tgcaggtaaa
     4741 aagcagtccc tcccaccaca agcggaagat tccctcgctc tctaatttct ctctggagat
     4801 atatcgcttc ttttttaaac tggaaagcac tccatctctc tccggggtcg aggaaatcca
     4861 cgagatgatg aggaactatt tctcgaaagc tggccggagg cttatcggta cctatatcca
     4921 ggtatttata aactgctaag gaatcagcat aaatcaactc cactttcccc agtcgctcag
     4981 ccagagctaa agaaaggtca gttttcccgg tagcagtagg accaacgatg gcggggagct
     5041 ttttaccttt taactgaacc ataaacttta ttgccctctc caggggttaa aactacctcc
     5101 ctccactccc ctacctccgc agaggagagt tctttcaggt atacccgcag attattagcc
     5161 cctctacccg ataacaggcc tccttcttcc tgatcaatca atatttccat tacttttccc
     5221 tgcagggagg gtaatttttc ctggtagata cttcgcagag tatcgttcaa aatatggaga
     5281 cgctcttttt tttcacagac aggaatatca tcttctaaaa gataggctgc agtacctggc
     5341 cggggagaat aaacataggt ataagccaca tcaaactgcg cctccctgac cacatccaaa
     5401 gtttcccgga aatcagagtc cgtctccccg ggaaagccca caatgatatc tgtcccgatt
     5461 ctcgccgaag gtatagactt acggatgaag ttgattttat tcagataatc ctcccgggta
     5521 taaccccgcc tcataagctg taaaatctta ttggagccag cctgaagggg aagatgaaaa
     5581 taagggcaaa gcaccgagct atcttttact atttttacta tgtcctctgt gaagtctcga
     5641 ggatgagaag taagaaaacg aacccagatt tttatacccc ggagccactc ctcgatatcc
     5701 agaagcaact cctcaaaagc tccttctctt tctctatccc gaccataact attgacattt
     5761 tgcccgagga gggtaacttc tcgaaatcct tcctctacca ggttttttag ctcctcttct
     5821 attatttctc ggggcttgct cctctgtttc ccggtggtat aaggaaccac acaatagcta
     5881 cagaaattat cgcaaccaaa agtaataggt aaataagcag aaattttact ggaataccgg
     5941 tatcctttct cctcataaag agaaggaggc tcctcctgga aatccactat ctttactttt
     6001 tcctgccagg cctttttaat tagaaggggt attttctccc aattatgagt gcccgccaga
     6061 acatttattt ctctatcctt cccaaggagt ttttccctct ccagctgtcc caggcatcca
     6121 cataccacaa ttatagagtc tggatttttc tcctttctct cccgagcaaa tttacttata
     6181 atacttatcg ctcgttctgc agcatgctct cgcaccgcac aggtattaaa tacgagaata
     6241 tctgcttccc ctgattcctc agcaggggag aaccccagct cctgcagtcg ataagacagg
     6301 tgttcagaac gacttttatt catctgacaa ccaaaagttt ccacccagaa agtagcttca
     6361 gccacttttc cggccggaaa atccggtttt cttttaatct ccactaaatt catagcaaaa
     6421 cactcaagcg cttcgaggtt tcaccaccag tttaatagct gttctctcct ccccatcaat
     6481 attgatatcg gtaaaagctg gtatgcaaac gaggtcgata ccgctgggag caacataccc
     6541 ccgtgctata gcaatagctt taatagcctg gtttactgct cctgctccta ccgcttgtac
     6601 ttccactatg cctttttccc ggatggctcc ggctaaagcg ccagctacag cattgggatt
     6661 agacttagaa gatactttta ggacatccat ggccattcct ccttcatttt ttttctaatt
     6721 ttaagatatg gataagcgag agatttccgt agctcttccc gtaatctcat ctacctccac
     6781 tacaatacca ttaagcttaa cgttgtcttt agccactcga tatctgatag gcatttgagt
     6841 tagaaatctg cttaaaatgt cttctgtttt taccccgaga accgaatcat gagctcctgt
     6901 cataccaatg tccgtaatat aggctgtacc ttgaggaaga attcgctcat cagcagtagc
     6961 cacatgggta tgagtgccaa aaacacaaga tactttacca tccagaaacc atcccatggc
     7021 tatcttctct gaagaagcct cagcatgaaa atcaagcaaa atcatgttgg ttatcctcct
     7081 gaagtcctcc agctcttcct ctatccgacg aaatggacaa tccagggaag gcataaacac
     7141 ccggccactc atattagcca ctatccattt tttacccttc ccttccactg ccacccagcc
     7201 ccggcccgga actccagaag ggtaattgag agggcggata agccggggtt ggtaatcaat
     7261 atagtctacg atttcctttt tatcccagat gtggttgcca gttgtaatca catctactcc
     7321 ataactcaaa atttcctcgc acacttccgg cacaattccc agacccccgg cagcattttc
     7381 tccgttgaca atcactccct cgatatttaa tttttcctta ataacttcta agttatcagc
     7441 cagtacttgc cgtcctggct ttcccactat gtctcccact attaaaaatc gcaaaagttc
     7501 attcctccat tatttagcat actctacggt tctggtctct cgaatcactg ttattttaat
     7561 ctgtccagga tattccagtt ctttttctat tttacgagct atatcataag ccagcttcgc
     7621 cgctcgggca tcgtcaatcc tatcaggctt caccatgatt ctaatttctc ttcccgcctg
     7681 aatggcataa gatttctcca ctccttcaaa accatcggca attttttcca gtttttccag
     7741 acgcttgata tacgactcca ggctttctct tcttgctcca ggccgagaag ccgatatggc
     7801 atcagctgcc tgcaccagaa ctgcttccac agtttgagct tccacctctc catggtgagc
     7861 ctcaatggca tgcactactt cttttttctc tccatatcga gaggctaatt ctgctccaat
     7921 tttagcatga ggaccctcca cttcatggtc taacgcttta cctatatcat gtaaaagacc
     7981 agctctttta gctaaagtgg tatccactcc cagctcgcta gccattaagc tggcaaaata
     8041 agctacctct ttggaatgtt gcaaaacatt ctgtccgtag ctggttctga aatacaactt
     8101 acctaaaagc tttaccagct cactattaac gttcttcact cccgcatcaa aaagagcttg
     8161 ttctccttcc tgaattatct tctcttccac ctgtagcttg gccttgtcta ccaattcttc
     8221 tattctcgcc ggatggatac gaccatcaac aatcaatttt tctaaagcta agcgcgccac
     8281 ttctcttctt atagggtcaa aagaagaaat gagcaccgct tcaggagtgt catcaatgat
     8341 taaatccacg ccggtaatca tctcgaaagt cctgatgttg cgtccttctc ttcctataat
     8401 tcttcccttc atttcatcac tgggcaaatt aaccaccgat acagtatttt ccactgtgaa
     8461 atcagcagca taacgttgaa tggtttgagc tacaacctct tgagccattt tccctgcctc
     8521 tcttttagcc cgctcttctg cttctttgat cttcacccct acttcgtagt ccagagtttt
     8581 ttctactcgc tccagcaatt cctgtctcgc ctcttcccag gaaagattag caatccggga
     8641 taattcctcc acttctcgcg ctttaatact ttccactact tctttttctt tttccagctc
     8701 ttcctggcgc ctgcgcaaga ggttctccgt tttctctacc tgttccaatt ttcgctccag
     8761 catttcttcc cggcgcaaaa tccgggattc gaaattctgc agttcttttc tcttccgctg
     8821 tatttcttct tccagagatt ttctctcttc taaaacttcc tctttagcta aaattaaggc
     8881 ttctttcctg agagtttcta cctggccaag cgtttcttct ctcactctct ctatttcttc
     8941 ctgagcaatc tttctctctt tctgaagctg ggagttcaag aagctaaatc ccgctaccaa
     9001 tcctacggct attcctatca aaatatatat gagctccata ttctggattc accccttatt
     9061 cacttttcaa tgtgcgattc atgtaggtaa aagaatattt taacggtacc ttaaggccaa
     9121 gtcaagattc ttccctgtac cacgcttctt ctactacttc ccaggaaaaa ccccgacgaa
     9181 gaagacgatt attaatgtct tctttttcct ttccctcccc tgaccattct ctgattagcc
     9241 ctcgagcgtc ttttacctct tcttcccggg ggaaaaattc ccgcagagaa tcgagtagct
     9301 cgtgagaaac acccttccta cgcaactcgt cctttatagc ttgtaaaccc cgaggattca
     9361 gtcttcttct tgtctctaca taaatcttta caaattcctc atcatttaag taaccacgct
     9421 cttccaaaaa ctgaagtgct tcttctatct cttcacgaga aaagccatct cgagctaatt
     9481 tctggaaaag ctcgcctcga gtatacatct tccggttgag atagcgaaaa ccctgtcgaa
     9541 tgatatcttt cacgaattaa aagcaatcac cgggtcaact tccatctgag aaagagcagc
     9601 agagagaatt tctttcgcta cttccggatt ttcctttaag aattgccggg cgttatctcg
     9661 accctggcct aaacgatatt ctccaaaaga gaaccagctg cccgattttt taactactcc
     9721 cgttgtctct cccaggctca atatttctcc ctcctgggat attccctcac cataaatcaa
     9781 ttctaactcc gcttctttaa atggagaggc tactttgttc ttaaccactt taattctggt
     9841 cagggcacct attatttcat ctcccgatct cagatactct cttcttctca catctaacct
     9901 tacactggca taaaatttga gagccctgcc acccggtgtg gtctccgaag gcccataagc
     9961 catagctatt ttttcccgga gctggttaac gaaaatagcc acggttttag ttttactgat
    10021 ataggcagtc aatcttctca aggcttgaga catcaagcga gcctgcaggc caatataaga
    10081 atcagacatg cccccttcta attccatctg aggaactaaa gcagctaccg aatcgagcac
    10141 cactatatcc actcctccgg tacgcaccag ggcatctact atatctaaag cctcttctgc
    10201 gcttccaggt tgagaaataa gtaatctgtc aatctggacc ccaattttgc ttgcatagct
    10261 ggggtctaaa gcatgttctg tatccacaaa ggcagcaata ccaccttgct tctgagcttc
    10321 agcaatggca tgcaaagcaa tggtggtttt acccgaagct tcagggccaa aaatctctac
    10381 cactctccct cgaggaaatc ctcctactcc cagagcaata ttgagaagaa ccgacccagt
    10441 ttttatcact tctatatcag cagttaaaac atcttgagat aagtacataa tagcgccttt
    10501 gccatatttt ttttctatag agcttatagc ctggttaatt acatcttctc tcaaatcttt
    10561 agagcttttt tcctcactca ttttttttca ccccttctaa actaaaaaca cctaattgct
    10621 ggtaaaccga accccgaggg gttaaagtac tttgataaaa aaccagtttt ttaatttcgg
    10681 tagaaaagtt gatattctct acccctaaag agggaagagc aaaaggagaa cgaaagcgag
    10741 ccagggtaat gtggggagaa aactctctat cttctctctc ccatccctta gcagctattc
    10801 ctttttcaac ttcctctctt atacttttta tctttccctc tccctctctg tccaccccta
    10861 tccacaccac tcgagcccga tgaagagaag gaaaagcccc ccatttctct aaagttacag
    10921 ggaaagctgt aaatttgttg cttacctctt ccagtatttt attcatttcc tctacctcac
    10981 caagaggaaa agcatgaaaa aagtggagcg taagatggag ttgctctctt ttaacccatt
    11041 ttgcttgagg gtaaagtttt ttctcctcgt taatcaactc ttctaagaag tcttgagctt
    11101 cagagggaag gtctaaagcc acaaagcttc tgatggtagt tgaactcatt accaaatttc
    11161 tcctttaaat attgaaaaat cataaccaga gcggattgag aggcaaatct tttgttcatc
    11221 aatctatccc ctgaaaaatg gaatttttgc actctcacgc cttcttcgct cgatagagca
    11281 atataaacca ggcctacggg cttttcttta cttcctcctc ctggccctgc aataccagta
    11341 atcccgagac ccaaatctac ttttccctta tggcgcactc cgcgagctaa aaatcgagca
    11401 gtttcttcac ttactgctcc tttttcttgc aaaatttcct cgggaacttt taaatcttcc
    11461 tttttagcct cgttactata gaccacgtat cctcgctcaa aatactgaga gctacccgct
    11521 atatcagtta tccgattagc taccagccct ccggtgcacg actctgccag tcccagtttc
    11581 aagccttttt ccttcatcat gttgcccacc gcttcttcta aagtgtcacc atagggactg
    11641 aagaaaaatc tatccaggtg gcgagctacc tcctctaaaa aaataagggc agactctttt
    11701 tcctctggag aaaagcggga gctgtaaaca tagagccaca cttctccata gttaggaaga
    11761 aaggctaaat tgaaagaaga gggcagatga cgaagaaacg gttccacctc tttagccaga
    11821 ttagattctc caatcccgca aaatttgaag ataggagaac gataaaaata ttcctcttcc
    11881 cggggaaggt ctttctctat taagctccag aaaaactccg cctcccgagg gacaccgggt
    11941 atgacaaaaa tacttttccc gttcacctct aacttaaaac cgggagcagt tccccgctca
    12001 ttataaagta tctctgctcc ttgaggaata aaagcctgcc ttctattatt ttccggaggc
    12061 tcggtattcc tcagttcccg atagaaaaac tgaattctat cccaggcttc agggctaaaa
    12121 atcaaacttc tgccaaaagc ttgagccact gcttcccggg tcaggtcatc ttcagtaggt
    12181 cctaaccccc ctgatacaat caccagcttt acttcctctt ccttcatgat aaattgtaaa
    12241 gcatctacta tcttctccat ctggtcaggc acaaaaagaa taaattgagg caatatccct
    12301 ttatctacca gtttttgagc cagaaagtag gcattataat ccctgactaa cccggtcaac
    12361 agttctgttc ctataccaag aatagcggct ttcaaatttt ttctcctttt aattcatagc
    12421 tactgctttc agtaacctta acttttacta tctcccctct ttttaattta ttcctccggg
    12481 agctaaagct tatattccca tctacttccg gagcgtctcc gtaggtcctt ccccaaaaat
    12541 tagtaactcc attacctctt tgagcttcgt ctactattac ttccaattcc cgacccacta
    12601 aagaactatg aaactttccc atcacttttt cctgcacttc cataagtgct ttgtagcgct
    12661 cctgtttcac tttctcctcc actttaggat aaaggaaagc tgaaggtgta tcaggtggtt
    12721 cagaatagac aaaagctccc attcgctcaa agcgaaaatc ttccacaaaa tcaaccaagc
    12781 gctgaaaagc ttcctcgtct tctcctggaa aacccactaa aaaagtggtc cgcaaaaata
    12841 tgccgggaac acactctctc agtttttcta acttttctct tattttttct atcgatgaag
    12901 ggcgcctcat cttctggagt atagtagaat ccacatgctg aagaggtata tccagatatt
    12961 ttacaagatg agaagaatct ctcatcgttt ttataaattc atcatccagc ccttcgggag
    13021 aaaaatacaa caaccttaac catactccag agggcaaaat ttcgtccaac cggcgaagaa
    13081 ggggcgataa catcctctgg tgatagaggt ctaagccata agaagatagg tcttgagcca
    13141 ccaggattat ttctttcact ccttgagata ccagattaag agcttctttt tctatcgact
    13201 ccatagttct actgcgcaaa gaaccccgga tagaaggtat taagcagaaa ctgcagtgat
    13261 gattgcagcc ttcggctatt ttgagataag cataatgaga aggagtagaa agaattctct
    13321 ctgtttcgtg gtggtaaaga aaaggagggg aatctaaaaa gtactttccg gggaacttgt
    13381 cttccagaat atcaacgagg gaaggaaaat cattcactcc cacccagtag tctacagcag
    13441 gatatacaaa accttttttt ctcccccagc gctgggcata gcaacccata accagtaact
    13501 ttttgggagg atgagcttct ttgagaaaag acaatttctc aatccatgcc tcagtttccc
    13561 gacaggcttc ctcaataaaa gcacaggtat tgagtatcaa tatttctgct tcttccggag
    13621 aagaaaaaaa attatatcct cgagaaagca atttacccag ggctacttcg gtatcaacta
    13681 aatttttatc acaacccaaa ctgataaagg caatttttct cacggctcaa atacttggcg
    13741 tgatgcttcc tctttctcac tgagataacc ccaatctttt ccatttacta ccgctttaac
    13801 ctcattccct ccttttattt ctacttctat gggataattg ctttttacaa catattcctt
    13861 gttgggaact aatattccag aaaaaacctc attccctccc gaccagattt ctgcccaggc
    13921 tgctttttca ggaaaaaggg taacggtgag agaaaattga ggtccaactt ctgcagaagg
    13981 agaaggaaga atctcttctg gctcaatcgc cgaaaccggg ggattaacca ctacttcttc
    14041 tccttcgggt aaagaatgaa aactaaaaaa gatgaagagg aaagtaaaaa acactgccag
    14101 agaaactaaa agaaccagaa gccttttaaa cagggtagat ctatcgctct ttctatctct
    14161 ggcgcttcta agctccggtt cctcggtttt tttccaatcc agacttttcc aatccacagg
    14221 ctttaccgaa taaacctgat tgaagagaga aaccatctct tcctcattta atcccagaaa
    14281 acgactgtaa atcttcaaat atcctaaagc ataagccctt cctggcaatt tatcccaatc
    14341 ttcttcttct aaagcggcaa tgtatcgaga ggaaacatgt gtaactccac ttacctcatc
    14401 acgggtaagc tctctttctt ctcttttttc ccgcaaaatt tctcctaatt taagttgtcc
    14461 ttctgccatt ttatccctct cctttgggag taattacttt tcgaggttta cttccttcat
    14521 aaggaccaac aatgccctcc cgttccatct gctcaatcag acgagctgcc cgattaaagc
    14581 ctactctcag tcttctctgc aaaagggaag tggaagcttt gccgctttct attaccactc
    14641 taaccgcttc ctggtaaagt tcatcctctt cttcctctcc cactcctctt tcttctcgag
    14701 gaacatattc cagaggatag taaatgtctc cagactgatt caaccagcat tctaccagct
    14761 ttttagtttc tgaagtactg acaaaagctc cttgcccccg gatgggttta ctcgctccca
    14821 gaggagcaaa aagtaaatct ccattgccta aaagtttctc cgcccccggg ctatccagga
    14881 tagttcggga atccacctga gaagaaacgg caaaagccag acgagaggga aaattggctt
    14941 taattaagcc ggtaataacg tccaccgagg gtctttgagt ggccacaaca agatgcattc
    15001 ccaccgcccg ggctatttgt gctatccggc acagagaact ttccacctcc gtaggagcag
    15061 tcatcatcaa atcagccagc tcatctataa ctatcaccac ataggggagc ttttcccctt
    15121 cttctaccat ctggttatat tcctctatat ttcgggcaga aacttcagaa aggagctcat
    15181 aacggttatc catctcccga catagccatt taagaaatct caccacccac cgagcctcag
    15241 aaataaccgg ggcacacagg tgaggaagcc cggaataaag agaaagctcc actcttttgg
    15301 gatctacaag gccgattctc aaatcgttag gagtagctcg atagagaagg ctggtcaaaa
    15361 tggaattgat gcacacactt ttacctgaac cagtggctcc ggcgatgaga agatggggtg
    15421 cctctcgtaa atcccagacc agaggcctac cgctcacatc tctacctaaa gcaacggtaa
    15481 gaggagattc acttttttta aactcttcac tttcaatcaa atctcgcaaa gaaacaagct
    15541 ctttatgacg gttaggaact tcaatcccca ctgctgacct tccaggaatg ggcgcctcaa
    15601 ttctcaccgc agcaacggca aaagccagag ctaaatcatt agaaagactg gtaatttttt
    15661 gcactttaat tcctggagca ggctgaaaat caaaacgagt tatagtggga cctacctgca
    15721 catttaccac tttccccagc acgttaaact cggctaaagt ttgttctaat ttatctattt
    15781 ctccaattat ctctctttta gttttctctc ccccccgaga aggatagacc cttaaaatct
    15841 ggggggaagg aagagtccag gtggtacttt ttttctttct ggaggaagaa gtgagtttct
    15901 ttttagcttg agcttcttct ttagccttca attccagcgg agcaaaaacc tcatctaatg
    15961 cttccccttc ccaggaagaa gaggtgattt ctttctcctt tacctttatt ggccgggaaa
    16021 ctccagacga agcaatttct ctttggggaa tttctctcct gcgagaaaag agctctcccc
    16081 ctttcctctt caagaaacgc cagccctgtg aaagctgacg gagtagcttt ccctctcctc
    16141 ccagataaac ggctaaaaaa gctgtaaaaa agagcgcaag aatagtgccg gtgtccccga
    16201 gataatacac cagatgacga tagagaaacg ctccgactat tccggcaaaa tccccatcag
    16261 aaaaggaaaa gcctgccctt tctgcggctc gctggctcaa ggtgagaaaa attactatcc
    16321 acagagagaa gctaattatc cggctcgtaa ctctaaactt acgagcgggc tgaaaatggt
    16381 aaatctcata agccagatag cccagaaaaa agggaagaag gtaagcacct actcctaaaa
    16441 agcgaaaaag gtaaaacccg attaattccc ctaactctcc cacatcaaaa gagaaaacga
    16501 tgagcaggct atatatggca aaaaaactca ggaaacaaac aactaaaagt tttttattca
    16561 cttcgcctaa ctccaaagat gtatttacct ttctctggat cgatgtctcg gttctttact
    16621 ccggttgctt ttctccgaat tatcgctatc acccagggct tctttgtggc tcagggcaat
    16681 acgacccata gagtctattc ccgttacttt taccagtacg tcatctccca ctttaacaaa
    16741 gtcttccacc ttttttcctc tttctgagga aaactgggaa atatggcaca aaccttctct
    16801 ccccggaaat atctctataa aagctccaaa gttagttact ctggtaactc gacccagata
    16861 cacttgaccc acttccacat cctgagtgat atttttaatc atattaatgg ctttattcat
    16921 accttcttcg gtacgagaaa aaactattac tttcccatca tctttgatgt caatagtggc
    16981 tcctgattct tctacgattt tcttgatgac tttccctccc ggaccaatta aagcaccgat
    17041 gcggtcaggg tcgacctcca gaatttctat ccggggagca taaggagata cagaactccg
    17101 gggtcgg
//
LOCUS       DAOPSS010000024        16658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_2919_length_16658_cov_7.843161, whole genome shotgun sequence.
ACCESSION   DAOPSS010000024 DAOPSS010000000
VERSION     DAOPSS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 16658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16658
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2919_length_16658_cov_7.843161"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            626..1876
                     /locus_tag="PKN31_02755"
     CDS             626..1876
                     /locus_tag="PKN31_02755"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOA98883.1"
                     /translation="MENQKEQQQSIVLLIIFLLALATRLLLAPFIPGYVTDVRCFKGW
                     ADQVAKVGFSRFYSSDMFVDYPPGYILVLYPIGKIRLLFNLDFNSPLFLLLVKLPSLI
                     TDLATGYLLLCAARKAKLNFSLSLLLILYLFNPAILINSAVWGQVDSFFTFFVLLSLW
                     FIEEKRFPLASSTMVYALLVKPQALFFFPILLFALLENKNWKTFFKSVVSGLVVLFLV
                     ILPFSWGNGIYWLIQKYQGTISSYPYASLNAFNFFALTGGNWVEETHPFLFLNYKTWG
                     YIFLLTISGTGAFLFFRWKNPYKIYPLSFFLISSIFMFSSKMHERYLYPALILALLVF
                     VYFPHRRILLTYSGFSITHFLNVGLVLLYSWYNVFHLPRYSPYVLAISLSNLLLYVYA
                     LYAGFHLFQNEKPMTSKESQVLSP"
     gene            complement(2356..2520)
                     /locus_tag="PKN31_02760"
     CDS             complement(2356..2520)
                     /locus_tag="PKN31_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98884.1"
                     /translation="MGQVVKLPQLKSWKNFLELFLFEKKAEGKAPRTIHNYKFHVSSF
                     FKHFALRKRA"
     gene            complement(2614..2690)
                     /locus_tag="PKN31_02765"
     tRNA            complement(2614..2690)
                     /locus_tag="PKN31_02765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2654..2656),aa:Arg,seq:ccg)
     gene            complement(3376..4017)
                     /locus_tag="PKN31_02770"
     CDS             complement(3376..4017)
                     /locus_tag="PKN31_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOA98885.1"
                     /translation="MKILAVSDRIDPRVYSDFCQERFKDVECVISCGDLPEHYLDFIV
                     TNLNVPLFFVHGNHDPSGGKRILAGGTNLDEKVITYQGIIIAGLEGSYWYNGGIHQYT
                     EREMYFKYLRLLPSLRWCKIRYGRFLDILVTHAPPQGVHEGSDLAHRGFSTFTHIIKK
                     YQPRYHLHGHVHIYDHNQSREDQLFNTRVVNCYNYYIIEIDPSELSPKTSVAL"
     gene            4292..5455
                     /gene="dnaJ"
                     /locus_tag="PKN31_02775"
     CDS             4292..5455
                     /gene="dnaJ"
                     /locus_tag="PKN31_02775"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOA98886.1"
                     /translation="MKRDYYEVLGVSRNATSEDIKRAYRRLARQYHPDVNQGDSNAAE
                     RFKEINEAYQVLSDEQKRATYDRFGHSAFEGANQGNYGGFGADFDPFADFSNFGDIFD
                     FFFGGGTQYRRERRPRATKGQDIKIEITLEFEEAARGVEKEISFSRTEICSDCQGIGG
                     KKKVACPHCQGKGEVRHTQTSLFGSVVTSRPCSYCQGRGWIAEDLCSSCRGSGKLKKE
                     RKIKIKIPAGVDSGYRLRVAGEGEAGENGGPPGDLYVHIKVKPHPVLERKGSDLYYDL
                     ELSYPQLVLGDEVEIPTLRGTEKIRIPAGTESGASLRLRGKGLPDPRIGSRGDQIIRV
                     KLRMPRRLTLEHRQALEKLMELERVENSLEGNGDNRKQSGVFERLKEAFNRPE"
     gene            5458..6183
                     /locus_tag="PKN31_02780"
     CDS             5458..6183
                     /locus_tag="PKN31_02780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOA98887.1"
                     /translation="MSRFLFYSPLVTNEVILLSPEESHHLLVERIKPGSSVFLSDGKG
                     NLFRGIYEGEKRGRAQIRVEEKVGEEKRSFDLVVWQAVLKSPSRMDWVVEKLTEIGVS
                     QIGFFSSHRSLKSTIPPEKMERWEKIVLSACKQSGRTIFPDLIFLEQWEDFIFSLSNS
                     PGTVILADPEGNEALWSFLQERKERSFQIVIGPEGDLTREEREEILKKAQGKRIKLSK
                     KILRSETASLFSASVVTSFLEDT"
     gene            6180..7433
                     /gene="mtaB"
                     /locus_tag="PKN31_02785"
     CDS             6180..7433
                     /gene="mtaB"
                     /locus_tag="PKN31_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOA98888.1"
                     /translation="MKVAIKTLGCKVNQVESDILTTTFSQEGIEVVNFGEKADLYIVN
                     SCAVTREAENKTRQLVNRVLRNNLAPRVILTGCYARLISQEEKNPFEEPVFLLPDFPK
                     EKEILNLVGKWWGKKFTLAEYPLPSRARVWLKIEDGCDHFCSYCLIPHLRGNIKSEKK
                     EKIIRQAQALERRGIREIVLCGVNLGYYGKNRGEKELINLLEELVDNTTLVRFRLSSF
                     EPFLLDEEFIKRYFSLQDRVCPHFHLPLQSGSDRVLKSMRRGYDTAYYRDLVSLLRSY
                     CPAVAISTDIIVGFPGEEKEDFENTISFSQEVGFSRTHIFVFSPRPGTPAFLWEKEKG
                     IPKKEKKNREKILREVARETQKNYHRLFLGEKLKVLVESVQDGMGSGYSENYIPVRFR
                     GNPEEGEIVEVTIEKEENGFLEGKS"
     gene            7527..7649
                     /locus_tag="PKN31_02790"
     CDS             7527..7649
                     /locus_tag="PKN31_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98889.1"
                     /translation="MKKTELPAALNKSEKGRMKKTRTTERKKQTAYGVSKKERN"
     gene            8014..8547
                     /locus_tag="PKN31_02795"
     CDS             8014..8547
                     /locus_tag="PKN31_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOA98890.1"
                     /translation="MRKWWIILISVLCFLSFFPGKGYTQEELLSVRVIKCTRPHYLKA
                     EVISPDEGKEVSIKLAGIKSPIGRAEIYKKALKRVKELTLNQDLKFDFALGYSEEEEV
                     WVGYLYIPEGDEWKIVNAELLREGLVELDEKTAGENMLGYLISAQEEAQEQKVGLWSS
                     PEPQKREKREECPGCIR"
     gene            8535..9062
                     /locus_tag="PKN31_02800"
     CDS             8535..9062
                     /locus_tag="PKN31_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOA98891.1"
                     /translation="MHKIKKSLKPQNWFILLILGLSSAFLLYGCVPTPTPPEKAPDFT
                     LPDMEGNLFTLSEKEGQPVVLCFFKTDCPACIREVPYLNAIYQEYKNTHGLLVVGIAI
                     GEPQDEVQNFIDTHGVEYPVLLDSQEEAADSYGIRSVPHLFFIDREGYTLKIEDYTGG
                     LIDQNKLESYVQRII"
     gene            complement(9208..10851)
                     /gene="hcp"
                     /locus_tag="PKN31_02805"
                     /gene_synonym="priS"
     CDS             complement(9208..10851)
                     /gene="hcp"
                     /locus_tag="PKN31_02805"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOA98892.1"
                     /translation="MFCYQCEQTAGGTGCTNFGVCGKDPETAALQDLLIYAAQGISMY
                     AKRAQDLGSRDREVDVFVLEALFTTVTNVDFDPKRLEGLIHKAYQMKEKARNMYLAAC
                     AQEGISPEELSGPAAWVPASNFGELVKQGEEVTLEKDIERWGESVAGAKHLIIYGLKG
                     MAAYADHAQILGVEDEEIYTFFHRTLDYLASDNFTLDELLSRALQVGEYNLKVMALLD
                     EAHTSTYGHPVPTPVRITPVIGKAILISGHDLKDLEELLKQTEGRGINIYTHGEMLPA
                     HGYPGLKKYPHLVGNYGGAWQNQQKEFDVFPGAILMTTNCIQKPRESYKDRIFTSGLV
                     AWPGVHHISNRDFTPVIEAALREKGFSESEEEKNIMVGFARNAVLGVADKVIKGVKEG
                     NIRHFFLIGGCDGAKPGRNYYTQFAQMVPSDCLILTLACGKYRFNKLEFGEIDGIPRL
                     LDVGQCNDAYSAIQIARALADAFQTDVNSLPLSMIISWYEQKAVAILLTLLHLGIKNI
                     RLGPSMPAFVKDPVYQVLEEQFNLIPITTPEEDLRAILG"
     gene            complement(10881..11282)
                     /locus_tag="PKN31_02810"
     CDS             complement(10881..11282)
                     /locus_tag="PKN31_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOA98893.1"
                     /translation="MHLVQISEAASLAIHSMALLIQNRGGFLTVKDMAQECEASEAHL
                     AKVMQKLAKARLVTATRGPQGGYTLTKEGEEASILTIYEAVEGAIMPRECLLKSKICL
                     FSTCIFGGVIEKAEREIKEYFASKKLKDLVK"
     gene            complement(11365..12051)
                     /locus_tag="PKN31_02815"
     CDS             complement(11365..12051)
                     /locus_tag="PKN31_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOA98894.1"
                     /translation="MEKVILEDIPWEVEEGKLLRKLHLEEGSEDAQRVRELLREAKKV
                     SSPRGMYRVAYIDKKGENKITIEGITFCSKVLRTNLEDVYRVFPFVATSGRELEEWSS
                     RFEDMLEAFWAAEIKEWALRQAISHLLEDLKEKFHLEKVAAMNPGSLPDWPMEEQRKL
                     FALLGDPVKEVGVELTESCLMIPVKSVSGIWFPAKIDFENCQLCSRENCPGRRAPYDE
                     NLARKYQVRV"
     gene            complement(12711..14171)
                     /gene="gguA"
                     /locus_tag="PKN31_02820"
     CDS             complement(12711..14171)
                     /gene="gguA"
                     /locus_tag="PKN31_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA98895.1"
                     /translation="MEKISKAFPGVQALSEVDLEVYKGEILALVGENGAGKSTLMKIL
                     TGVFKKDEGKIIFKGKEIEARNPHHARQLGISMIYQEFSLVPNLDIATNIFLGNEPTR
                     KFDIFDYERAKTEAERLLKMVGLGVSPDVLVEDLTVAQQQIVEVAKALASESELIIMD
                     EPTSALSKEESERLFGIMKKLKEEGISIIFITHRLEEVFAVADRIVVLRDGRRVGELS
                     CEETRISEVIRLMVGRDVRVVPRVEVSSDDVVLEVRNLSRLPMIKDVSFSLKKGEVLG
                     IAGLVGAGRTEIMRALFGADLKTSGEIFIEGEKVEIQSPNDAVKMGIGLVPEDRKFQG
                     LILIMAVYENISLPSLPFMFSNGIINFEKEYSLAESLVERLSIRTPSIFQAVNNLSGG
                     NQQKVVLAKWLALKPKVLILDEPTRGIDVGSKAEIHHLIDELAKEGIGVILISSELPE
                     ILALSDRILVISKGRITKELKREEADQETIMQYAII"
     gene            complement(14255..15277)
                     /locus_tag="PKN31_02825"
     CDS             complement(14255..15277)
                     /locus_tag="PKN31_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOA98896.1"
                     /translation="MRKLKLSLIFALCFLILFVSAGYAEEIHVAVIGKSVHPYWAEVE
                     LGVKKAAEDLGVKATFFVPQKEDIQAQISQMESFIAMGVDGIAIAPSDPTALAPTIAT
                     AMSKGIPVITLDTDAPESERLAYIGTDNYSAGKLAGQVMSQLLGPEGGKVAIGTGSLT
                     AMNSLERMRGFTDGIAENENIVVVTKPALCDFEDTGRAVTLVEQALLTYPDLKGFFGV
                     YAINGPAAAKAIKTAGKVGEVLVVCFDTTAEHMQLIKEGAIAATIGQRPYMMGYKSVE
                     LLSKMVADGVDAALAELPEDRIVDTGVDVVAADAYVETLRVLEADSVEEYREKLIGLG
                     IPVEGW"
     gene            complement(15365..16321)
                     /locus_tag="PKN31_02830"
     CDS             complement(15365..16321)
                     /locus_tag="PKN31_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA98897.1"
                     /translation="MTKRKNSKSFRFREMNILIALILMCLLITIMNPRFLNPFNLQTI
                     ARQVAIYGLLAVGETLVIITGGIDLSVGSLVALIGVLVALFMKGGLSVAFSIIIVLLI
                     SSLIGLTHGLFVTKLNVPPFIITLGTLTAARGLASAITKGWPIIGLPESFFFIGQGHI
                     IGIPFPLIILLIFAAIAFFITRYTVLGRQLYATGGNIEAARLSGVNVDRRRIFAYVFN
                     SFLAGVTGIIVASRLSQGQAGVGSGYELNAIAAAVIGGASLAGGEGTILGALIGATIM
                     SVIYDGLILLEISSYWHDVVMGLVIVAAVTIDVWRKRKSKAG"
BASE COUNT         5005 a         3619 c         3225 g         4809 t
ORIGIN      
        1 atcctaaatg tatttatcac cctttaggtg tcactcctga atgtatttat caggaatcta
       61 cattatttaa ggacgaagac ctttagattc ccgattaaag cgctcgggaa tgacagaaaa
      121 gtactataag ggggcgctgc cccctcataa ccccctgaaa aaaacgacaa atatgaaaaa
      181 caaaaactac tgaggaagga aatgcgtctt cctcagtaga tgcctgatta aggatttcag
      241 gcatgacaga aggggaagcc cttgaaggga gacccctgtt tttccttctc ccttgattcc
      301 ttttcctttc cctcattttt agttagggtg agggtgaagt ttccacaata atcttatttg
      361 caacagctta ctcctctcct tatctctccc gcaaaaatag taagaaaatc cagggttgtc
      421 acgggaagga atatacgacc ctcacgatga cgccccggaa taaattgcca tagtaatgcc
      481 ctggcacaaa aaatgaataa agcataaaaa gaaacattac catggatttt gcggttaact
      541 atggctgact atgaattgca tggtaaaata ccataaaaac gtaaataatc ttatgaagcg
      601 ctatttattt ttcgatgagg acaaaatgga aaaccagaaa gagcagcaac aatctattgt
      661 tttgctcatt attttcttgc tagccttagc gactcgcctc ttactcgctc cctttattcc
      721 tggatatgtc accgatgtaa ggtgttttaa aggatgggca gaccaggtag caaaagtagg
      781 attttctcgg ttttactcaa gcgatatgtt cgtcgactac cctccaggct atatcttagt
      841 tttataccct atcgggaaaa ttcggttgct atttaatcta gatttcaatt ctcctctctt
      901 cctcctcctg gtaaaattac cctctttgat taccgatctg gctaccggat atttgctcct
      961 ctgtgctgct cgaaaagcca aacttaactt ttctctttct ctccttttga ttttgtatct
     1021 ctttaaccct gccatcctta tcaattctgc tgtctgggga caggtcgatt ctttctttac
     1081 tttcttcgtg cttctttctt tgtggttcat agaggaaaaa cgttttcccc ttgcctcatc
     1141 tactatggta tacgctctcc tcgtaaagcc tcaagccctt ttctttttcc ctattttact
     1201 cttcgcgctt ttagaaaata aaaactggaa aaccttcttt aagagtgtag tttctggatt
     1261 ggtggtactt ttcttggtga ttcttccttt ctcctgggga aatggaatct actggctgat
     1321 tcagaaatac cagggaacta tttcttctta tccttatgct tcactcaatg cttttaactt
     1381 ttttgctcta accggaggga actgggtaga ggaaactcat ccttttcttt ttctcaacta
     1441 taaaacctgg ggctatatct ttctcttaac tatatcagga actggggctt ttttattttt
     1501 ccggtggaaa aacccctata aaatctatcc tttaagtttt ttcctcatca gttctatttt
     1561 tatgtttagt tctaaaatgc acgagcgcta tctatacccg gctctgattc tggctcttct
     1621 cgtttttgtt tattttcccc atcgccggat tttgctcact tattcagggt ttagcataac
     1681 ccatttccta aacgtggggt tagtgctcct ttatagctgg tataatgttt tccacctgcc
     1741 ccggtatagc ccttatgttt tagccatttc tctatctaac ctccttctat atgtttatgc
     1801 tctttacgct ggttttcatc ttttccagaa tgaaaagccg atgacttcta aagaaagtca
     1861 ggttctatct ccctaaccaa ttaataattc cagaactacg ggataaagag tcctgtgcca
     1921 atatatgcaa aaacattttc tttaatggtg tgaacgggag aaacatcttt ctccagcaaa
     1981 ctctatgctc ctcagaccct gaaaatccgc tttagcatct caataaacac tttccccgga
     2041 aagggaaact taacctctct tcttctgctg aggagttata atcaaaagtt gcgtatggcc
     2101 aaatgaaaac aaaaacaggc ggcaatactg gggtgaaatt tcaaagaaaa aatatcacct
     2161 taatttctaa aaggagaata cgccatgaaa aaagaaaagc agattaagga gaaactaagc
     2221 aaaataaagt ctaccgggtt tttctcaata acgttctctc tcactaagaa atcaaagaaa
     2281 gctttaagat attttcggtg aagattaaaa gtagcaggtt taatatcatc ggagaaatac
     2341 ttgaggatgg aagctctaag ctctttttct gagcgcaaag tgtttaaaaa agcttgagac
     2401 atgaaactta taattgtgga tagttctcgg agctttacct tcagctttct tttcaaacag
     2461 gaacagctct aaaaaatttt tccagctttt gagttgcgga agttttacta cttgccccac
     2521 agaaatcgcc tctttctggg agtcgatttt atagtcactg gactcagggg tttttagaga
     2581 gagtagagaa ggcgaaaata gctctgtgaa ggctggcgcg cccgagagga tttgaacctc
     2641 cagcctacgg ctccggaggc cgtcgctcta tccgattgag ctacgggcgc atgcccaaaa
     2701 tcctgattta cttgctacta ttttatattt tatgcttttc tctgtcaatg aatcgttaag
     2761 gctatttgaa aaatcatgcc ttctacagaa cataattcct tcttatactc aagatacatg
     2821 tttatcttta taggtcttga actttcccct ttataacccc gatcgtaggg gtagacccct
     2881 ataaacccct gaaaaaagga caaaaacaaa aggcagaaaa tataaaaaca aagtccactg
     2941 aggagcgtca cattcctttt taggatattt ttattttcct ctgttattag cttttaaatc
     3001 tctctctgtt cagtttcagc attgtttata accagaacct cattcagaaa aagcctttag
     3061 ttaagctctc gaggctcgaa agcaactggt aactgacttg tcataggctt ttatagcgat
     3121 ttctcccctt tttccccctt cgcagtaatt tcttggtcca gagcaaaggg agaaagattt
     3181 tgatgataga ttctgaagca ccaaagtatg aaaaacaact tctattcctc tccaatctgt
     3241 ggataccggt atattgacct taaccttcac atgatatgtg ttctgcaaaa tatgcaccac
     3301 taaaccagat gacgaggtct ctctggccat gattaaaaat gccacaattc tggagacaga
     3361 tactggcttt tagcttcaga gtgctacgct ggtcttaggg gaaagttcag aaggatctat
     3421 ctcgattatg taatagttgt agcagttgac aacccgggta ttaaaaagct ggtcttctcg
     3481 actttgattg tggtcataga tgtgaacgtg gccatggagg tggtaacggg gttgatattt
     3541 tttgatgata tgggtaaagg tggaaaaacc ccgatgagcg agatcgcttc cctcatgaac
     3601 cccctgggga ggagcatggg ttaccagaat atctaaaaac cttccatagc ggattttaca
     3661 ccaccggagt gagggaagta accgcaggta tttaaaatac atctctcttt cggtgtattg
     3721 atggatgcct ccattgtacc agtaggagcc ctctaaccca gcgataatta ttccctggta
     3781 agtaatcact ttttcatcta aatttgtccc tccggccaat atcctctttc cccccgaggg
     3841 gtcgtgattt ccatggacaa aaaagagggg aacgttgaga ttggtaacta tgaagtcaag
     3901 atagtgctca ggaaggtctc cgcaggaaat gacgcactct acgtctttaa acctttcctg
     3961 gcaaaaatca ctataaactc gggggtctat ccgatcgctt acggctaaaa tcttcattta
     4021 ttgaattata caataggttt gaaattatgt taagaaaaac tacgaattat agcgcaaaac
     4081 aacttacagc gtgagattaa aataaaaaac aaaaaaaatg agaaaaaact aaaataattg
     4141 cgtcgggaaa atatattccc gtactacagc gggaactctc atagctctct ccttgaaaaa
     4201 attttccata gaagggaaca ttacagcgtt ctttgtggtt aactatgatt tgtagtataa
     4261 tagggagcga atttaaaaaa ggtgaaaagc tatgaaacga gattattacg aagtcttggg
     4321 agtaagcaga aacgctacct cagaagatat aaaaagagcc taccgtcgcc tggctcgtca
     4381 atatcaccct gatgtcaatc aaggagatag caacgcggca gaaaggttta aagagattaa
     4441 cgaagcttat caggtgttat ctgatgagca aaagagagct acttacgatc gttttggcca
     4501 ctctgctttt gaaggagcta atcagggaaa ctatggggga tttggagcag attttgaccc
     4561 ctttgctgat ttttctaact ttggcgatat ttttgacttc ttctttggtg gaggaactca
     4621 gtatcgtaga gagcgccgcc ccagagcaac gaaagggcag gacataaaaa tagaaattac
     4681 cttagaattt gaagaagcag ctcgaggagt ggaaaaggag atttctttct cccggacaga
     4741 aatttgttcc gattgtcagg gaataggagg aaagaaaaaa gttgcctgtc ctcattgcca
     4801 gggaaaagga gaagttcgtc atacccagac ttccttgttc ggttcggtgg tcacctctcg
     4861 accttgctct tattgtcagg gtcggggatg gatagcagaa gacctttgtt cgtcttgtcg
     4921 aggaagcggt aagctcaaaa aggagagaaa aattaaaatc aagatccccg ctggagtgga
     4981 tagcggttac cgcttacgtg tggctggaga gggagaagcg ggagaaaatg gaggaccacc
     5041 gggtgactta tatgttcaca ttaaggtcaa acctcatcca gttctggaga gaaaaggctc
     5101 agacctctat tatgatctgg agctgagtta tcctcaattg gttctgggcg atgaagtaga
     5161 aatacccacc ttgcgaggca cagaaaagat ccgtattcct gctggaactg aaagcggagc
     5221 ttcattgcgt ctgcgaggta agggattacc tgacccccgg attggtagcc ggggagatca
     5281 aataatcaga gtcaaactcc gtatgcctcg tcggttaact ctggagcacc gacaggctct
     5341 ggaaaagctt atggaactgg agcgagtaga aaacagcctc gaaggaaacg gcgacaatcg
     5401 caaacaaagc ggagtatttg agcgcttaaa ggaggctttt aatcgccctg agtaataatg
     5461 agtcgtttcc tcttttactc tcctttggta accaatgaag taattttact ctctccagag
     5521 gagagccatc atcttttagt agaaagaata aaacctggtt cttctgtctt cctgtccgat
     5581 ggaaaaggaa acttatttcg gggaatttat gagggagaaa aaagagggag agcccagata
     5641 agagtggaag aaaaagtagg ggaagaaaag cggagttttg atcttgtggt gtggcaagcc
     5701 gttttgaaaa gtccctcccg gatggactgg gtagtagaaa aattgaccga gataggagta
     5761 tcacagatag gttttttctc ctcccatcgt tcacttaaaa gcactatccc tccagaaaaa
     5821 atggaaagat gggagaaaat cgttctgagt gcctgcaaac aatcagggag aacaattttc
     5881 cctgatttga tatttttaga gcaatgggaa gattttatat tttcccttag caattctcct
     5941 ggaacagtga ttttagccga cccggaagga aatgaagctc tctggtcttt tctccaggag
     6001 agaaaagaga ggagttttca aatagtgata ggaccagaag gtgacttgac cagagaagaa
     6061 agagaagaaa ttttaaaaaa agctcagggg aaacggataa agttaagtaa aaaaattttg
     6121 cggagtgaaa cagccagcct tttttctgcc tcagtagtaa cttcttttct ggaggacaca
     6181 tgaaagtagc gataaagact ttaggatgta aagtaaatca ggtggaaagc gatatattaa
     6241 ccaccacttt ttcccaggaa ggaatagaag tggtaaattt tggagagaaa gctgacctgt
     6301 atatagtgaa cagttgtgca gtgaccagag aagcagaaaa caaaactcga caactggtaa
     6361 acagagtctt gcgaaataac cttgcccctc gagtaatatt gaccggctgt tatgcccgcc
     6421 tgatctctca ggaggagaaa aatccatttg aggagccggt ttttttgtta cccgattttc
     6481 ccaaagaaaa agaaatttta aatttagtgg gaaaatggtg ggggaaaaag tttactcttg
     6541 ctgaataccc cctcccctct cgagctcgtg tctggctcaa gatagaagat ggttgcgatc
     6601 atttctgcag ctattgctta attccccacc ttcgaggcaa tataaaaagc gagaaaaaag
     6661 aaaaaattat aaggcaagct caagcacttg agagaagagg aataagagaa atagtccttt
     6721 gtggagtgaa tttaggctat tacggaaaaa atagaggaga aaaggagctc atcaaccttt
     6781 tagaagagtt ggtggacaat accactctgg tgcgttttcg gttgagctct tttgagccat
     6841 tcctgctgga tgaagaattc attaagcgtt acttttcact gcaagataga gtttgtcctc
     6901 attttcacct tcctctgcag agtggtagcg accgagtttt gaaaagcatg aggagaggct
     6961 acgacactgc ttattatcga gatcttgttt ctctgctgcg cagctactgt cctgcagtcg
     7021 ccatttccac tgatatcata gtgggatttc ccggagaaga aaaagaggat tttgaaaaca
     7081 ccatcagctt ttctcaagaa gtaggctttt ctcgaactca catttttgtg ttttctcctc
     7141 gcccgggaac gccagctttc ctctgggaaa aagaaaaggg aatccccaaa aaagagaaaa
     7201 aaaaccgaga aaaaattctc cgggaagtgg ccagggaaac tcaaaaaaat tatcaccgcc
     7261 ttttcctggg tgaaaagctg aaagtcttag tagaatcagt acaggatggg atgggctcag
     7321 ggtattctga aaattatata ccagttagat ttagaggaaa ccccgaagag ggagaaatag
     7381 tagaggtaac aatagaaaaa gaagaaaatg gttttttaga agggaaatca taactcctct
     7441 acataataaa agtttagaaa aagcagccca cagcgtagaa aagaactata agggagtgaa
     7501 atcccatatg gctgactccc ggaagagtga aaaaaacaga actacctgca gcactaaaca
     7561 aaagtgagaa aggcagaatg aaaaaaacaa gaactactga gagaaaaaaa cagacagcct
     7621 acggcgtaag caaaaaagaa agaaactgaa aacgcagaag aacaaaaact aatgagagga
     7681 taaaataccc ctcggtagat ttccgattaa agatttcggg cctgacaaaa aaagaacaat
     7741 aggaaaacct cccctataac ccctcgaaaa ggacaatata gaaaggctga ataataacgc
     7801 tgaaaaaata tcgtggtgtt gagagataaa aagtgtctct cccgacttgc gataaaccgt
     7861 tcaggaaatt accacataga cgaaaagacg agccccaatg acagttcaga aagaatgtat
     7921 tactgtagaa aagaatattg caatggtttt gcggttaact ataattgaaa ctatataatt
     7981 taagaagtcg tatactcaag aggaggtgaa aaagtgcgga aatggtggat tatcttaata
     8041 tcagtattat gtttcctttc tttcttcccc ggaaagggat atactcagga agagctttta
     8101 tcagttagag taataaagtg tacacgcccc cactatctaa aagcagaagt catctctccc
     8161 gatgagggaa aggaagtttc gataaagtta gcaggaatca agtctccaat agggcgagct
     8221 gaaatctata aaaaagcgct aaaaagagtg aaagagctaa ctttaaatca ggacttaaag
     8281 tttgattttg ctttaggcta cagcgaggaa gaggaagtat gggtggggta tctttatata
     8341 ccggaaggag atgagtggaa aatagttaat gctgaactcc taagggaagg gttggtagaa
     8401 ttagacgaaa aaacagcagg agaaaacatg ctgggttatt taatttctgc tcaagaagaa
     8461 gcacaggaac aaaaagtagg attgtggtct tcgcctgaac ctcaaaaaag agaaaagaga
     8521 gaggagtgtc ccggttgcat aagataaaaa aaagtttgaa gcctcaaaat tggttcattt
     8581 tattaatttt aggactgagt agcgcttttt tgctttacgg ctgcgtacca acaccgactc
     8641 cccccgagaa agcaccagat tttactctcc cggacatgga aggaaacctc tttactttga
     8701 gtgaaaaaga aggacaaccg gtagtacttt gctttttcaa gaccgattgc ccagcttgta
     8761 ttagagaagt accttacctc aacgctattt atcaagaata taaaaatact cacggacttt
     8821 tagtggtagg aattgcaata ggtgagcctc aagatgaggt gcaaaatttt atagatactc
     8881 acggagtaga atatcctgtt cttcttgact cccaagaaga agcggccgat agctacggga
     8941 ttcgctccgt ccctcacctt ttctttattg accgggaagg atacaccctt aaaatagaag
     9001 attatactgg tggactaata gaccaaaata aattagaaag ttatgtgcaa agaataattt
     9061 agtcatctta caattaaagt attcagcata atgtgaaagc aaagacagta gagacactca
     9121 aagattagac tttgcaaaga gggtatcata gcaccatgtg cccacctata ataaaacacc
     9181 ggggaaggaa aaatcccccg gtgtttttta tcctaatatg gctctcaaat cttcttctgg
     9241 cgtggtaata ggaataagat taaattgttc ttcgagtact tgataaaccg ggtcttttac
     9301 aaaagcaggc atacttggcc ctaagcgtat attttttatt cccagatgaa gaagagtgag
     9361 taaaatagcg actgcctttt gctcatacca ggaaataatc atagaaagtg gaagagagtt
     9421 aacatcggtt tggaaagcat cagctaaagc tcgggcaatc tgaatggcag aataagcatc
     9481 attacactgc cccacatcaa gaagtcgagg aataccatca atttctccaa attctaattt
     9541 attgaagcga tatttaccac aggccagggt gaggatgagg cagtctgaag ggaccatctg
     9601 ggcaaactga gtgtagtaat ttctccctgg ttttgcccca tcacatcctc caatcaaaaa
     9661 gaaatggcga atattgcctt cttttactcc tttaatcact ttatctgcta ctccaagaac
     9721 tgcattacgg gcaaatccta ccattatgtt tttctcttct tcgctctcag agaaaccctt
     9781 ttccctcaga gcagcttcaa ttaccggagt gaaatcccga ttacttatat gatgtacccc
     9841 cggccaggct accaaaccac tggtaaaaat tctatcttta tagctttccc tgggtttttg
     9901 aatacaattg gtagtcatca gtatagctcc agggaaaaca tcgaattctt tttgttgatt
     9961 ctgccaggca ccaccataat ttcccactaa atgaggatat tttttgagac ctggataacc
    10021 atgagcagga agcatctccc catgagtata aatattgata cccctaccct cagtttgttt
    10081 gaggagttct tctaaatcct tcaagtcatg cccggagatc agaatggctt tacctatcac
    10141 aggagttatt ctcaccggag tgggtacagg atgaccatag gtagaagtat gggcctcatc
    10201 cagaagagcc atcactttca aattatattc tcccacctgc aaagcacgag agagaagctc
    10261 atccagagtg aaattgtcac tggctaaata atcaagggtg cggtggaaaa aagtatatat
    10321 ttcctcatct tctactccta aaatttgcgc atgatcagca taggctgcca tacctttaag
    10381 tccataaata attaaatgtt tagccccggc taccgattct ccccatcgct ctatatcctt
    10441 ttccagagtt acttcttccc cttgttttac cagctcacca aaattagaag ccggaaccca
    10501 ggcagcagga ccagaaagct cttccggtga aattccctct tgagcgcagg cagcaagata
    10561 catattcctg gctttctctt tcatttgata agctttatga attaacccct ccaatctttt
    10621 cggatcaaaa tccacattgg taacagtagt aaaaagagct tccaggacaa aaacatcaac
    10681 ttcccggtct cgacttccta agtcttgcgc tcttttagca tacatagaaa tcccttgagc
    10741 agcgtaaatt agaaggtctt gcaaagctgc agtttctgga tctttaccac acactccaaa
    10801 attagtacat cccgttcctc ccgcagtttg ctcacattga taacagaaca tgtaaggcac
    10861 ctccttacga ttttaagttt ctacttaact aaatctttca attttttact ggcaaaatat
    10921 tcttttattt ctcgttctgc cttttctatt actcccccaa aaatacaggt agaaaaaaga
    10981 caaatcttgc ttttaagcag acactcacga ggcataatag ccccttccac cgcttcataa
    11041 atagttaaga tactagcctc ttctccctct tttgttaaag tatatccacc ctgaggacct
    11101 cgggtagcag tcaccaatct cgctttagct aatttttgca ttactttagc taaatgggct
    11161 tctgaggcct cgcattcttg agccatgtct ttgacagtga gaaagccccc tctattctgg
    11221 attaaaagag ccatgctgtg aatggccaag gaagctgctt ctgaaatttg aaccagatgc
    11281 atttttttct cctttcaggt attatgatac ctaaatacct aaatttgtca agaaacgatt
    11341 ctaaaaaatt tttccaggga aaaattagac tcttacttga tattttctcg ccaggttttc
    11401 atcataagga gctcttcttc ccgggcaatt ttcccgagaa cagagctgac aattttcaaa
    11461 atctatttta gcaggaaacc agattcctga aacagattta accggaatca tgagacaact
    11521 ctcggttaac tccactccca cttcttttac cgggtcaccc aacaaagcga aaagctttct
    11581 ttgttcttcc atgggccaat caggcaggga gccaggattc atggctgcta ctttttccag
    11641 gtgaaacttc tcttttaaat cctcgagcag atgagagata gcttgtctca aagcccattc
    11701 ttttatttct gccgcccaaa aagcttccag catatcctca aatcgactgg accactcctc
    11761 caattccctt cctgaggtag ccacaaaagg aaaaactcga taaacatctt ccagattagt
    11821 cctcaaaacc ttgctacaaa aagttatccc ttcaatggta attttgtttt cccctttttt
    11881 atctatgtaa gccacccgat acattcctcg gggactgctc acttttttag cttctcttaa
    11941 caactctctg actctctggg catcctcact tccctcttct aagtgaagtt ttctcaaaag
    12001 ttttccttct tctacttccc agggaatatc ttccaaaatt accttttcca cagcttttcc
    12061 tccctcttct atattaaatg actcaattct accatattgc tattatcttt aaatactctt
    12121 ggattcctga tagaaacatt caggaatgac agataagaga agcaacagaa cccactcagg
    12181 agtggcaaca cggagatctt ttcctccgtg ttattttttt atctttctct tttgtctttt
    12241 tgtatttttg tcttttcagg ggggttatga gggggaatat tccccctcat agttatttca
    12301 ccttcaccct ctccctccag agggtgaagg tgaagaagtc tcccgttaag gcttcccccc
    12361 tcctccgtcg ttcccaaaat tcttaattgg gaatctaatg agggtatttt atcccctcat
    12421 tagtttttgt tcttctgcgt tttcagtctc tttctttttt gcttacgccg taggctgtct
    12481 gttttttcct ctcagtagtc tttgtttttt tatgtctttt tgtatttttt cttttaactc
    12541 tttaaaaggg gccagaggga acctccctct atagttagag gtcataaagg ggtactcccc
    12601 tttattgttc tacgccatag gtagttctgt atttttgcct ttcagggagt gatagggggc
    12661 gcatcccccc tatagttcgg agttataatg aggacattct ccctgttttt ttatataatc
    12721 gcatattgca ttattgtttc ttgatctgcc tcttctctct ttaactcttt ggttattctc
    12781 cctttggaaa ttaccagtat tctatcagag agagcgagaa tctctggaag ctccgaagat
    12841 atgagaataa ctccaatacc ttcctttgcc aattcgtcaa ttaaatggtg aatctctgct
    12901 ttcgacccca cgtctattcc tctcgtgggc tcatccaata tcaaaacttt gggcttcaaa
    12961 gctaaccatt tagctaaaac cactttctgc tggtttccac ccgagagatt attaactgct
    13021 tgaaaaatac taggagttct tatactaagc ctttcaacaa ggctctctgc cagggaatac
    13081 tctttctcga agttaataat accgttggaa aacataaagg gcaaactagg aagagaaata
    13141 ttttcatata ctgccataat aagaataagg ccttgaaatt ttctatcctc aggaacaagc
    13201 ccaataccca tctttactgc atcgttgggg gattgtattt ctactttctc tccctctata
    13261 aatatctcac cagaagtttt aaggtccgct ccaaaaagcg ctctcatgat ttccgttctc
    13321 cccgctccta caagtccagc aatccctaaa acctctccct ttttaaggga aaagctcaca
    13381 tctttaatca tagggaggcg ggaaaggtta cgaacctcta aaacaacgtc gtcagagctt
    13441 acctctactc tgggtacaac ccttacatcc cttcctacca tcaatcttat gacttcactt
    13501 atcctggttt cttcgcaaga aagctctcct acccgtcggc catctctcaa tactactatt
    13561 ctatctgcaa ctgcgaaaac ctcttctaac ctgtgagtta tgaatattat agatattccc
    13621 tcttctttta actttttcat tattccaaag agcctttctg actcttcctt tgacagggca
    13681 gaagtgggct catccataat aattagctct gattctgaag ccaatgcttt tgctacttct
    13741 actatttgct gttgagctac cgtaaggtct tccactaaaa catcaggtga aacccctaaa
    13801 cccaccatct tcaacaatct ttcagcttcg gtttttgctc tctcgtaatc aaaaatgtca
    13861 aattttctgg taggctcatt ccccagaaat atattagtag caatatcaag attaggaaca
    13921 agactaaatt cctgataaat catagatata cccagttgcc gggcgtggtg ggggtttctt
    13981 gcttcaattt ctttaccttt aaatataatt ttcccctcgt ccttcttaaa aacaccggtt
    14041 aaaattttca taagagtgga tttgcctgct ccattttccc ctactaaagc taaaatttct
    14101 cctttataaa cctccaaatc cacctcagaa agagcctgaa ccccgggaaa tgccttggag
    14161 atcttctcca tcttcaatag taactcttgt tccaaatgta ttctcctccc aatccctcct
    14221 gataaaagag ggggggaacc cccctctcag tttcttacca gccttctaca ggaattccca
    14281 ggccgatgag tttttctcta tactcttcta cactatctgc ctctaaaacc cgcaaggtct
    14341 cgacataggc gtcagcagct acaacatcta ctccagtatc cactattcta tcttcaggca
    14401 attctgcaag ggcagcatct acaccatctg ccaccatctt agacagaagc tctacactct
    14461 tataacccat catatagggt ctctgtccta tggttgctgc aatggcacct tcttttatca
    14521 attgcatgtg ttctgctgta gtatcgaaac agaccaccag cacttctccc actttccctg
    14581 cagttttaat agcttttgct gcagcaggac cattaatggc ataaactcca aagaatcctt
    14641 taaggtccgg gtaagtgagt agcgcttgtt ctaccagagt tacagctctt cctgtatctt
    14701 caaagtcaca gagtgcaggc tttgtcacta ccactatatt ttcattttca gctatgccat
    14761 ccgtaaaacc tctcattctc tctagggagt tcatagcagt gagagaacca gtacctatgg
    14821 ctaccttacc cccttcaggt cctaagagtt gtgacatcac ttgccctgcc aatttaccag
    14881 cactgtagtt gtcagttcca atataagcta gcctttcact ttcgggagca tcggtatcca
    14941 gggtgattac cggtatcccc ttgctcattg ctgttgcaat agtgggagca agagcagtag
    15001 gatcagaagg agcaattgct atcccatcaa ctcccattgc tataaatgat tccatttgag
    15061 aaatttgtgc ctgaatgtcc tccttttgag gtacaaagaa tgtcgctttc acccccaggt
    15121 cttcagctgc tttctttact cccagctcta cctctgccca gtaaggatgg acagattttc
    15181 caatgactgc tacgtgaatt tcttcagcat accctgcaga aacaaacaaa atcaggaaac
    15241 aaagagcaaa aattaaggat aatttaagct tcctcatttt actgaacctc ctttttaagt
    15301 atttttatgt tagttctaaa acagaaaatg cttaaaatct tatcaccccc cttaaacatt
    15361 tgcctcaccc cgccttactc tttcgctttc tccagacatc tattgttaca gctgcaacaa
    15421 taacaagacc cataaccaca tcatgccagt aagaagaaat ctccaaaaga atcagcccgt
    15481 cgtaaataac gctcattatg gtagctccta taagagcccc caggattgtc ccctctcctc
    15541 ctgcaagaga tgctcctcca attactgcgg cagctatagc atttaattcg tatccgctac
    15601 ccactcctgc ttgtccctgg cttaatcttg aagcaacgat tattcctgta actccagcca
    15661 aaaaggaatt aaatacgtaa gcaaatattc tccttctatc cacattaact ccagaaagcc
    15721 tcgccgcctc aatatttccg cctgtagcat agagctgcct tcctaaaacc gtgtatctcg
    15781 taataaagaa agcaatagca gcaaaaatca ggagaattat aagagggaag gggataccta
    15841 tgatatgtcc ctgccctata aagaagaaac tttcgggaag gccaataatg ggccatcctt
    15901 tcgtgattgc tgatgccagc cctcttgcag cagtaagagt tcctaaggta attataaatg
    15961 gaggaacatt gagcttagtc acaaacaaac catgtgtaag cccgataagg gaagagataa
    16021 gcaaaactat tattatagaa aaagccacgc ttaaaccgcc cttcataaaa agagccacca
    16081 gcaccccaat aagagctacc agagagccca ccgaaagatc aattcctcca gtaataataa
    16141 ctaaagtctc ccccacagcc agaagtccat atatagcaac ctgtcttgca atcgtctgaa
    16201 ggttgaaagg gtttaaaaag cgggggttca taatggttat taataaacac ataaggataa
    16261 gggcaatcaa aatgttcatt tccctaaaac ggaagctttt ggagtttttt cttttagtca
    16321 tttcttgttg catagtagat cagcttaccc tcctctcgta taccttactc taaatattaa
    16381 tgacaaccat gctaaaagtc aacccctcgg gggctctata ataaagtgtg tgaaaaaata
    16441 tctaaaaaga agtatgggtg gtaaagatct cacaatggga aatcaaaaag aaagaagacc
    16501 ttaccaccat ggccagaggg aaacaatttt atcgggggtt aaagacttaa aaatagaata
    16561 gcagttaaag cttcaattaa aaaggagaaa agggaaatgc ccgctctccg gatcggtaaa
    16621 actctataaa catacacaga aagagggaag ttctccat
//
LOCUS       DAOPSS010000025        16105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_3048_length_16105_cov_8.526480, whole genome shotgun sequence.
ACCESSION   DAOPSS010000025 DAOPSS010000000
VERSION     DAOPSS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 16105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16105
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3048_length_16105_cov_8.526480"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1154)
                     /gene="ychF"
                     /locus_tag="PKN31_02835"
     CDS             complement(60..1154)
                     /gene="ychF"
                     /locus_tag="PKN31_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOA98898.1"
                     /translation="MGLKVGIVGLPNSGKTTIFNLLTKAKAPVASYPFCTIDSNVGIV
                     EVPDERLEVLASLLHPPRVVRATIEFIDVAGLVEGASKGEGLGNQFLSNIRGVDAVIE
                     VIRFFQDEKVPHALGKIDPLQDKEIVDLELMLSDLEIMERRLEKLQDLTRKVKDERLK
                     LEKEVLEKGKRWLEEGKPLRFLEFKEEEKEILKPYQFITLKPLIYVANLDESESSKYL
                     FEEVKKELEESGEKIIPVFAKLEEELEEIPVEERKVFIQEFGIEDTGLNLVVKEAYQL
                     LGLITFYTFGDKELRAWELLRGSKAPQAAGKVHTDMEKGFIKAEVINYEELVAIGSWG
                     KSREEGRIRQEGKDYEIKDGDLVYFRFSPH"
     gene            complement(1158..1385)
                     /locus_tag="PKN31_02840"
     CDS             complement(1158..1385)
                     /locus_tag="PKN31_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOA98899.1"
                     /translation="MAVIKLEIGDILTLKKAHPCGSKEWKVVRVGVDIGLRCMGCDRF
                     VMVSRRKLEGKIREIYRKGHRLKPQESIIEA"
     gene            complement(1397..1912)
                     /locus_tag="PKN31_02845"
     CDS             complement(1397..1912)
                     /locus_tag="PKN31_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOA98900.1"
                     /translation="MDFKELIQTRRSIRKFRAEEVEEEDLREILEAARLAPSASNLQP
                     WKFIVIKSDKKKRLIAQAAGGQEFVGEAPVVIIACATGRGGFIGGYMESWPVDVAISV
                     THLLLAAWDRGLGTCWIGDFDEEKVKEICEIPPEVRVVAIVPMGYPVKIPQATSRRPW
                     EKIVSMETFGE"
     gene            complement(1986..4196)
                     /locus_tag="PKN31_02850"
     CDS             complement(1986..4196)
                     /locus_tag="PKN31_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA98901.1"
                     /translation="MKNWLNFIIALVALIISVVLVFQVSGWKTSLVDLEKRMGGVEEK
                     AKKVEDSLSQIQDKIQSLPVDLLDWGERLGKIEASIAKTDESIANFISVMEELKGEFA
                     PRDEVIQRIEGELENWKKELEKINQLVLEAQNQFLSQAEWESAASQWEERFKKAEEEI
                     SNLESYLEVIQEQQFQDLEKLKATVESEVKAIEERANLLEQSLEEAWVSLKKEIDKLN
                     IDQRFTMLLDRINEQSAEIDSSIRELEKRIDALENLFQDLQGAMEKLESVQTQQSQQI
                     EDVRSLLERETTSLEEKIIFLENQGKEAEDILRLLVQEKEKLKETIDTLHQSLSSVED
                     ELNTISLQWDSKWQGISEDISNWQEATLEDIEAKIVSIKQELPDRSELEEWEKKINIL
                     QEEGERLKRELVRLEGEKDRALQEGEERLSKLEEEILLWEKELADYQKLMEQSISEIN
                     KQVSELTTFLNSAEERLREIETYSVNWSKESQDAWESLKISLSQLSERLEEVSNFSQN
                     VLKKELEAKIDSIEEKIEEMRKELNKEKQTLEISHSVTSTTEEDGETSEEGVVFSSGM
                     GGEEVLLVEEPLESKEKLSAEIKTLAEEKMELEEELSKKQEEMLKIDQELEELREEKG
                     SWEKIEELEGEKVEKEKAIADLNQKLEEKNAQIEALQREITELTAQLEEAEKYTSYVI
                     LPWDNLWDIARRYYRDGRKWPLIMEANKDVIEDPLKLRPYTEIRIPRCSNDEDC"
     gene            complement(4346..4480)
                     /locus_tag="PKN31_02855"
     CDS             complement(4346..4480)
                     /locus_tag="PKN31_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98902.1"
                     /translation="MVFSSLPDILRKSLVGSNEDRDREDWGKKGFEVQNWGLKGILQT
                     "
     gene            complement(4592..5458)
                     /locus_tag="PKN31_02860"
     CDS             complement(4592..5458)
                     /locus_tag="PKN31_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98903.1"
                     /translation="MEGALRIQDIIGLILAILIVAILISIILILRENARERRMWREED
                     YEDWREQKSKRDFSFSSREQEGVSPILEDRIPEELEKRRGWDALPRQREVEEIRKEEK
                     KESVIPPPPEEVFTPQEEPIIARESVRRGEPKQVVLERALRKIEELGVDINSLKPQIT
                     EDTIHFSPLLWEVREEVRAGRELGDMVIHTVFKNIPGYSRDEVVELYRSGDVSVVEKL
                     EARVKTSVGRDYLVATLDLEHFRIPDLERLWRKRSLEDEEYELLLSLRFVDRNTKKDF
                     ARVVYARITAED"
     gene            complement(5469..5996)
                     /locus_tag="PKN31_02865"
     CDS             complement(5469..5996)
                     /locus_tag="PKN31_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOA98904.1"
                     /translation="MEEVLKQVANFMAVSARTAPKSGGKDFINIQIVKGEEVKRLGEA
                     MVDCGEKQGIKGFIRDGKNVLDSPVVLLIGIKDAAVMNLNCSGCGYSSCSELQAAEKR
                     EADFKGPQCAFRLLDLGIALGSAVKTASLFNVDNRIMYRIGAVARKIGISQDDVVIGI
                     PLSAEGKSIYFDRKE"
     gene            complement(6011..6901)
                     /locus_tag="PKN31_02870"
     CDS             complement(6011..6901)
                     /locus_tag="PKN31_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA98905.1"
                     /translation="MTFVVNYHKENIINHLWDRLADCDLCPRNCGVNRLRGERGFCGG
                     GTLAKVASFHPHRGEEFLLSGSQGSGTIFFSGCNMRCVYCQNYSISQRREGEELTEKE
                     LTRIMLYLQNRGCHNINLVTPSHFIPQIFRSLLKARENGLTIPVVYNTGGYDKKEVLE
                     KIEGLVDIYLPDMKYAREESAYKYSEAQDYPRINREAVREMFRQVGEVEWGEEGVAKR
                     GLIVRILIIPSLEDEARENLRFLAENVSQNVFISLMRQYRPCYKADLFPELSRSVDER
                     TYREIVAYARELGLRNLILQ"
     gene            complement(7298..7639)
                     /locus_tag="PKN31_02875"
     CDS             complement(7298..7639)
                     /locus_tag="PKN31_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOA98906.1"
                     /translation="MELVREEDKAYRFGDWGIKYLFRGPRIDWGVFYLKPGTNLGAHY
                     HNQVEETFFILEGKGLLRTEGQEIALEKGCALYLAPGEKHDLYASEDSFLRGIFIKSP
                     YLPEDKVDCSI"
     gene            complement(7680..8822)
                     /locus_tag="PKN31_02880"
     CDS             complement(7680..8822)
                     /locus_tag="PKN31_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOA98907.1"
                     /translation="MKENFVFRLPPEIYFGGGEGKKAGELVKRWGEKALLVTGKKSMQ
                     ETGILEEILKSLEGAGVQCSIFDQVEPEPSLETVDRGLDLAYRERCDFVISLGGGSAQ
                     DCGKAIAGLCGNSHTVLPYFEGKVLSSPGIPWIAVPTTAGTGSEMTTNAVLTDYRTKL
                     KKSFRSPYLLAKLVIIDPLYTRFMSPYLTATSGIDALVQALEAYVSQEVNPVSEVLAE
                     EAIELLWKYLPQAVKEGQNLNYRKQVAKGSMISAMAFANSASGPAHGLSHIVGPEFSV
                     VHGEACGIFFPRVVEFNREVLKDRYEKIARIVEIEGEEDLIDKFIEAFLNLMKEIGLR
                     TSLGELGIKEDMLQGIVKEERIGRNLRENPRPMGEEEIYTLLKASL"
     gene            complement(8819..9355)
                     /locus_tag="PKN31_02885"
     CDS             complement(8819..9355)
                     /locus_tag="PKN31_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98908.1"
                     /translation="MRKKEKISPRQEEILLFIQDYYRKNGYPPTVREIGEGVGLLSSC
                     TVHYHLEKLEEKGMIRRNRFKARTIELCFPDREDGLVDVPFLNSLSRGPVSLPRNFLG
                     EGDFFFWQVEKNNWQDWHILKGDWLLIRKEEEIREEGLALIIKSDGGKIKRIDGNLSL
                     SADDGEIIGRIMGVWRKL"
     gene            complement(9361..9594)
                     /locus_tag="PKN31_02890"
     CDS             complement(9361..9594)
                     /locus_tag="PKN31_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOA98909.1"
                     /translation="MSVQAYILVQVQTGKAQAIKKELTQLSQFLRVDRVMGPFDLIAL
                     VEVENNREIGELILKEIQSLSGVKRTLTCPIIP"
     gene            complement(9617..10864)
                     /locus_tag="PKN31_02895"
     CDS             complement(9617..10864)
                     /locus_tag="PKN31_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOA98910.1"
                     /translation="MKVKEDYLREIKERAEKALNKKAPLGPDVDLNEFYLCSSQEEVD
                     SVESISASLREAALYAGVELEENKSSATYLQVDRSAVYQKIQDAFQGKLEIMAVSEAL
                     EKYPELLEYWWGLVPVDADKYTAFSEICPMEGYFIRVFAHQKIEVPLQACLLLSEESR
                     IQSVHNLVILEEGAEANLITGCASVRGNESGVHIGVSEFYLGDNSRLTFTMVHNWGEN
                     FHVRPRTVVKMGNNSFYSNTYVLLKPLSSLQTFPQAIMEGENADANFQSIVFAKGDSY
                     IDVGSSLLLKGGGCRGNSISRVCAADSSKVYTRGKLISYHDEAQAHLECKGILFSQRA
                     EIISIPELEVHGAPRSRLSHEAAVGPIEEEAVNYLRSRGLSREESLSLITRGFLNIDI
                     PGIPVVLKQYIDEVIRLTSRDIM"
     gene            complement(10861..11586)
                     /locus_tag="PKN31_02900"
     CDS             complement(10861..11586)
                     /locus_tag="PKN31_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA98911.1"
                     /translation="MLEIKELGVRVGGEEVLQDVNLEIGPGEVHILLGPNGAGKTTLL
                     MAIMGMPGYEVTRGDILFQGESILPLTVEERAKMGIGMAFQKMPVIPGVKLRTLGEII
                     VNGGSSLIEEVAEKLNCTYLLERSIGLGFSGGEAKRAELFQLFLQRPLLSLIDEPESG
                     VDLDNIALIGKALSELLEREQVKTKKRSGLIITHTGYILEYLNADVGHVLMEGRIVCR
                     GNPRDLFVDIREKGYQECLRCER"
     gene            complement(11629..12774)
                     /locus_tag="PKN31_02905"
     CDS             complement(11629..12774)
                     /locus_tag="PKN31_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOA98912.1"
                     /translation="MDRTNQVWERAMPVSEEEIISLVKQFDTPLFVVSEEKLLQNYRA
                     FQEFFPGIEIFYAVKANPHPFIIRTLKEIGSSFDVASFQEIELVSSQGVPPERMIFAN
                     TIKRKKGLRYARQIGVNLMTYDNEEELKKIAMVYPEAQLLVRIKTPSNGSRANLSYKF
                     GAEPDMAPELLLQAKNMGLKPAGISFHVGSPCSNVNNYLYSLQKVREIVEKVSGEISI
                     SVIDIGGGFPLSLYEEEGEGSLEAFSKAINPHLQEFLKDGYRVIAEPGRVIVGSAAFL
                     ITKVIGKAVRSGITWYYLDDGIYGTFSAIPFDKAAFRFLPLRESKEELPAVLAGPTCD
                     SLDVVAENVLLPPLELEDLIVVPDIGAYSWASATNFNGFERAQVVMI"
     gene            13376..14374
                     /locus_tag="PKN31_02910"
     CDS             13376..14374
                     /locus_tag="PKN31_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOA98913.1"
                     /translation="MDLRVLSREDIFSPRPEWRFEPEAPDYFAMYSSLWGGIVKDPAL
                     MLIPLDDHMVHRGDGIFEAFKCVKGHIYNFDAHLSRLENSARLISLNLPFDLETIKEI
                     TLKTVAIAGVKDCRIRIYVSRGLGNAFSCSPVDCRSQLYVVVLKESVPPPYYYTDGAS
                     AITSCIPTKASFFAQVKSCNYLPNALAHLEAHEKGADFAIMLDQQGYLAEGPTQSIAI
                     VDHQKIFKYPRLDNTLKGTTLLRAVALAEELVEAGELKSISSSDISREEAYNSLEMFA
                     FTVGIAVVPIVKYDEKVIGTGKPGPVFRRLLKLFQRDYTENRELLTPVPYDEVANG"
     gene            complement(14479..15360)
                     /locus_tag="PKN31_02915"
     CDS             complement(14479..15360)
                     /locus_tag="PKN31_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98914.1"
                     /translation="MRRYFLLLVILIILGGGSPLPGQEAPQVLLLSSSLDYFSTFLEV
                     HLRRLLSPSRVHLIPLCSLEELSLGFPPEKAFLIVFLGEEKYFSSILSQIPQDKEPLL
                     LFWEGVAPPLGFPSCKIRVEVEEVIEVFRREVVEKLFLDDHFTIWGVGKIAPRLRQGL
                     NEEFYPRFREIGENDKDTLKQLISNREKSLIIAGNAEAGRQLIDKFSEVESPPPLVVL
                     EASPEMLFALLQGKIEAVVDFKPSQLAQEIANLIDCFCANKPVPEFITIHPRLILPHN
                     IEEADGYEVISRCLMCQ"
     gene            complement(15357..>16105)
                     /locus_tag="PKN31_02920"
     CDS             complement(15357..>16105)
                     /locus_tag="PKN31_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA98915.1"
                     /translation="AIVIGVLIGRVGLSPSLAVVIGMLTGLAAGLFNGLLVSVGKILP
                     LIVTLAGMYIYRSLALIIAVDPQFGPNPMPVSNFPKSFYFIGQYRIGGIPFQVLFIFV
                     PVLLILHFLLSRTAWGRKLYGVGSNETAATFSGIDVSRVRLTVYALSGLLSALAAWVI
                     TSRIASARPDIGTGYELQAITIAVLGGISIQGGEGTVGGVLLSVAIITMLNNGLQLAG
                     IHQIWQLGALGLVLIGSVLLNQFLIRRRKI"
BASE COUNT         4597 a         3996 c         2613 g         4899 t
ORIGIN      
        1 tcagtaaact ccagaaactc ttgaattact tcgctcttta gcaagcgtca gggtagctct
       61 caatgaggag agaagcggaa gtaaaccaaa tctccatctt ttatttcata gtctttacct
      121 tcctggcgaa tccttccttc ttcccgggat tttccccatg aaccaatagc tactaattcc
      181 tcatagttta taacttcggc cttaataaat cccttttcca tatcggtatg tactttacct
      241 gcagcctggg gggctttgct ccctcggaga agttcccagg ctcgcaattc cttatctcca
      301 aaagtataaa aagtaatcaa gcctaagagt tggtaagctt ctttcactac aagatttaat
      361 cccgtatctt caataccaaa ttcctggata aatacttttc tttcttcaac aggaatctct
      421 tctaactcct cttccaattt agcaaaaact ggtattatct tttctccgct ttcttccagt
      481 tcctttttaa cctcctcaaa aagatactta ctgctttcgc tttcgtccag attagccaca
      541 tagataagag gttttagagt tatgaattgg taaggtttca atatttcttt ttcttcttcc
      601 ttgaactcta aaaagcgaag tggtttacct tcttctaacc agcgttttcc tttttctaga
      661 acttcttttt ccagttttaa ccgttcatct ttaacttttc tggttaaatc ttgcaatttc
      721 tccagtcgac gctccataat ttctaaatcc gaaagcatca actccagatc cactatttct
      781 ttatcctgca aggggtcaat tttccccagg gcatgaggta ccttttcatc ttgaaaaaac
      841 ctgattacct ctattaccgc atctacgccc ctgatattac tcaaaaactg attgcccaga
      901 ccttcccctt tactcgctcc ttctaccagg ccagccacgt ctatgaactc gatggtggct
      961 ctcaccactc gaggaggatg taaaagagaa gctaaaacct ccagccgctc gtcaggaacc
     1021 tctactattc ccacgttgga atcaatagtg caaaaaggat aactggctac cggtgcttta
     1081 gctttcgtca aaagattaaa aatagtggtt ttccccgaat taggtaaacc cactatacct
     1141 actttaagac ccatgctcta tgcctcgata atactttctt ggggttttag acgatgtcct
     1201 tttcggtaga tttcccgaat ttttccttcc agtttccgtc gggaaaccat cacaaaccta
     1261 tcacagccca tacacctcaa gccaatatcc acccctactc tgaccacctt ccattcctta
     1321 cttccgcaag ggtgggcttt tttcaaagtc aaaatatcac cgatttccag cttaattacc
     1381 gccaaaatcg tcctccttac tcaccaaaag tctccatact cactattttt tcccagggtc
     1441 gacgagaagt ggcttgaggg atttttaccg ggtatcccat aggaactatt gctacaactc
     1501 tcacctcggg aggaatctcg caaatttctt ttactttttc ttcatcgaaa tcccctatcc
     1561 agcaagttcc cagacctcta tcccaggccg ccagcaaaag atgagtaaca gaaatagcta
     1621 cgtctaccgg ccagctttcc atatacccac caataaaacc acctcttcct gtagcacagg
     1681 caataataac cacaggagct tctcccacaa actcttgacc gcctgctgct tgagcaatca
     1741 gtcttttttt cttatcactt tttatcacta taaacttcca gggttggaga ttactagccg
     1801 agggagccaa acgtgctgct tccaatattt ctctcaaatc ttcctcttcc acttcttctg
     1861 ctcgaaattt tcgaatgctt cttcgagtct gtatcagttc cttaaaatcc atagatatct
     1921 actcctctct cctatgttca caagagtaaa gcttataaag ctacaaaccc tctcgcacaa
     1981 ttcagctaac aatcctcatc attagaacag cgaggaatcc ttatttccgt atagggtctc
     2041 aactttagag ggtcctcaat aacatccttg ttggcttcca taatcaaagg ccactttcgt
     2101 ccatctcggt aataacgacg ggcaatatcc cataaattat cccagggaag aatcacatag
     2161 gaagtgtact tctccgcttc ttccagttga gcagtcagtt cggttatttc cctttgcaga
     2221 gcttctatct gagcgttttt ctcctctaat ttctggttca aatctgctat agccttttct
     2281 ttttccactt tttctccttc taactcttca attttttccc aggacccttt ttcttctctc
     2341 aattcttcta attcctgatc tattttaagc atctcttcct gttttttact taactcttct
     2401 tctaactcca ttttctcctc agctaaagtt tttatctctg cagacaattt ctctttactc
     2461 tctaagggtt cttctaccaa taatacttct tcccctccca tcccggaaga aaaaacaacg
     2521 ccttcctctg aagtttctcc atcttcctct gtggtagaag ttacagagtg gctaatctcc
     2581 agggtttgct tttctttatt cagttctttc ctcatctctt ctattttctc ttctattgaa
     2641 tcaatcttgg cctccagctc tttctttaaa acattctggc tgaaattgct cacctcttct
     2701 aatctctcag agagctggga taaagaaatt ttcaagcttt cccaggcatc ctggctctct
     2761 ttagaccagt taacgctata ggtttcaatt tccctcaaac gttcctccgc tgaatttaga
     2821 aaagtggtta attcactaac ttgtttgttt atttcactaa tggattgctc cattaatttc
     2881 tggtaatctg ccagctcctt ctcccacaaa agaatttcct cttctaattt actcagcctt
     2941 tcttcccctt cttgcaaagc tcgatctttt tctccctcga gacgaacaag ctctctcttt
     3001 aacctttccc cctcttcttg cagaatattt attttctttt cccactcctc cagctcgctt
     3061 ctatccggta actcctgttt aatacttact atcttggcct caatgtcctc aagagttgct
     3121 tcttgccagt tactgatatc ctcgcttatt ccctgccact tactatccca ctgaagagaa
     3181 atagtgttta gctcatcttc cactgaggat agactctggt gcaaggtatc aatggtttcc
     3241 ttgagctttt ctttctcctg aactaagagg cgaagaatat cctccgcctc ttttccctga
     3301 ttttccagaa aaattatttt ctcttccaga gatgtggttt ctctttctaa aagagaacgt
     3361 acatcttcta tctgttgaga ttgctgagtt tgcactgatt ctaatttttc cattgctccc
     3421 tgaagatcct gaaacaaatt ctccagggca tcaattcttt tctccagctc ccggatggag
     3481 ctatcaattt ctgcactttg ctcattaatc cggtctaaaa gcatagtgaa acgctgatcg
     3541 atgttcagct tatcaatttc ctttttgaga gagacccagg cctcctccaa gctctgctcc
     3601 agaagattag ctctttcctc tatagctttt acttcggatt ccacagttgc cttcaatttt
     3661 tctaaatctt gaaattgctg ttcttgaata acttccagat aactttccag attacttatt
     3721 tcttcttctg cttttttaaa tctttcctcc cattgactgg ctgcactttc ccactctgct
     3781 tgactgagga attgattctg agcttctaac accagttggt ttatcttttc cagctctttt
     3841 ttccaattct ccagttcgcc ttctattctc tgaataacct catcccgcgg ggcaaactct
     3901 cctttcaact cctccattac agagataaaa ttagctatac tttcatcagt tttcgcaata
     3961 gaggcttcaa tctttcctaa tctttctccc caatccagta aatcaacagg aagagattga
     4021 attttatctt gaatttgaga tagagaatcc tctactttct ttgctttttc ttccacccct
     4081 cccatccttt tttctaaatc aaccaaagat gttttccatc ctgaaacctg aaacactaaa
     4141 actacagaaa taatcaaagc taccagagca attataaaat ttagccagtt cttcactgtt
     4201 atcacctcct agaaaaatta tagcattctt ttgctctaaa tcttaatatt taccaactta
     4261 atagatacct atctcgttaa atcggctcat ggtaaaaaaa attacccact ctccccaccg
     4321 gggtttttaa ggtcccgggc ttcaactatg tttgcaagat gcccttcaaa ccccaatttt
     4381 gcacctcgaa tcctttttta ccccaatctt ctctatctct atcctcattg ctaccaacta
     4441 aagatttacg aagtatgtca ggaaggctgg aaaaaaccat aatttacggt tctttacgat
     4501 ataaaagatt tctatcttta gtcctgaaat attcaggaaa aagatagaaa tcaccagaat
     4561 ttagaatggc aaatataact tctatacagt attaatcttc agcagtaatt cgagcgtaaa
     4621 ccacccgggc aaaatctttt ttagtattgc ggtctacaaa cctcaaagag agcagaagtt
     4681 cgtattcctc atcctctaag cttcttttcc tccacaaccg ctccagatcg gggatacgga
     4741 aatgttctaa atctaaggta gccactaagt aatctcgtcc cacgctggtc ttaactcgag
     4801 cttctaattt ttctaccacc gatacatcgc cagaacgata tagttctacc acttcgtctc
     4861 gagaataacc gggaatattc ttaaaaacag tatgaataac catatcaccc aattctcttc
     4921 ccgctctcac ttcttctcgc acctcccaca acaggggaga aaaatggatt gtatcttctg
     4981 taatctgtgg ttttagagaa ttaatatcta cacccagctc ttctattttt cttaacgctc
     5041 gctccagaac cacttgcttc ggttctcctc ttcttactga ttctctggct ataattggct
     5101 cttcctgagg agtaaaaacc tcttctggag gtggaggaat aacgctttcc tttttctctt
     5161 ctttccttat ctcttcaact tctctttgtc gaggaagggc atcccatccc ctcctctttt
     5221 ctaactcttc ggggattctg tcctcgagaa taggagaaac tccctcctgt tctcgagaag
     5281 aaaaagaaaa atctcgttta gatttctgct ctctccaatc ttcataatcc tcttcccgcc
     5341 acattctcct ctcccgggca ttttctcgga gaataagaat gatgcttatc aaaatagcca
     5401 caatcaaaat ggctaaaatc aaacctatga tgtcctgaat cctcaaagct ccctccatag
     5461 ttacacctct attctttccg atcaaaataa atactcttcc cctctgccga aaggggaatg
     5521 cctattacta catcatcctg ggaaatccct attttccttg ccactgctcc aattcgatac
     5581 ataatacgat tatccacatt aaaaagagag gcagttttga ccgctgaacc cagagcgatt
     5641 cctaaatcta aaaggcgaaa agcacattga ggacccttaa aatcagcctc tcttttttcc
     5701 gctgcctgaa gttcagaaca gctgctataa ccacatccac tacaattaag gttcatcact
     5761 gccgcatcct ttatccctat taacaaaacc actggagaat ccaaaacgtt cttcccatcc
     5821 cttataaatc cttttattcc ctgtttctca ccacagtcaa ccattgcttc gcccaatctt
     5881 tttacttcct ctcctttcac tatttgaatg tttataaaat ccttacctcc cgatttaggc
     5941 gcggtgcggg cagaaacagc cataaaatta gctacctgtt tcaaaacttc ttccaatttt
     6001 catacctcct ttattgtaaa atcaagtttc ttagccccag ttctcgtgca taagctacta
     6061 tctcccggta agtcctctca tctacactcc gactcaattc cggaaataaa tcagccttat
     6121 aacaaggacg gtattgcctc ataagagaga taaaaacatt ctgagaaaca ttctcagcca
     6181 ggaatcgcaa gttttccctg gcctcatctt ctaaggaggg gatgattaaa atcctcacta
     6241 tcaaccctcg cttcgctacc ccttcctccc cccactctac ttctcccacc tgacggaaca
     6301 tctctcttac tgcttctcta ttaatccggg ggtaatcttg agcttcagaa tatttataag
     6361 cactttcttc ccgggcgtat ttcatatctg gtaaatatat atctactaat ccctcaattt
     6421 tctccagaac ttctttttta tcataccctc cagtattata aaccactgga atggtaagac
     6481 cattctctcg agccttaagg agagaacgaa aaatctgggg aataaagtga ctgggggtaa
     6541 ccaggtttat attatggcaa cctcgattct gcaggtaaag cataattcgc gttaactctt
     6601 tttcggtaag ctcctctcct tctcttctct ggcttataga ataattctgg caataaacac
     6661 atctcatatt acaaccagaa aagaaaatag tacctgaacc ctggctacca ctcaaaagaa
     6721 actcctctcc tcggtgggga tgaaagctgg ctaccttcgc cagagttcct cccccacaga
     6781 aacctcgctc tcccctcaaa cgattcactc cgcaatttcg gggacataag tcacaatccg
     6841 ctaatctatc ccagagatga ttaattatat tttctttatg atagttaacc acaaaagtca
     6901 ttataattgc accccttcag ggggttaccg ggaaaggttt tcctttctga caatatttat
     6961 gtttcttgcg ttttttatca attattgctt gtttttttat tttctcaatc ttttagtgtt
     7021 tttaatcccg caccatcaga ctctctcgca atcccagact tttcacacaa tacaagcctc
     7081 tttttaaatt atattcagat ctgtcaggca aaaaaaacga ggctcacaat gaccagagaa
     7141 agtaactaac aggggaagcg tcctctttac agttttgccg caatcttgac cgatattcaa
     7201 tttccaaggt acacttgcat tcctctaaag cctcaatctc tataaactac cacccctatt
     7261 ttcctgccat ataacgatag gggagtgtcc cctatcgtta tatggaacaa tccactttat
     7321 cttcaggtaa ataaggactt tttataaaaa taccccgtaa aaaggagtct tctgatgcat
     7381 aaaggtcgtg tttttctcct ggagcaagat ataaggcaca tcctttctct aaagcaatct
     7441 cctgtccttc agtccttaat aatcctttac cttccaaaat gaaaaaagtt tcctctacct
     7501 ggttatggta gtgagcccct aaattagtcc ctggcttaag ataaaacact ccccagtcta
     7561 tgcggggacc tcggaaaaga tattttattc cccaatctcc aaagcgataa gctttatcct
     7621 cttctctaac taattccata agtcaagccc tcctcggatg ataaattgct ctctatttct
     7681 taaagagagg cttttaaaag agtatagatt tcttcttccc ccataggacg gggattttct
     7741 cgaaggtttc tccctatcct ttcctccttt actatccctt gaagcatgtc ttcttttatt
     7801 cccagctccc ccaagctagt tcgtaaacca atctccttca ttagatttaa aaaggcttct
     7861 ataaatttat cgatcaggtc ttcctctcct tctatctcca caatgcgagc tattttctca
     7921 tatctgtctt ttagcacttc tcgattaaat tctaccaccc gggggaaaaa aattccgcag
     7981 gcttccccat gaaccacaga aaactctgga ccaacaatat gagacaaacc atgagctgga
     8041 ccagaagcag agttggcaaa agccatagca cttatcatgc tccctttggc tacctgtttg
     8101 cgataattta gattctgtcc ttctttcact gcctgaggaa gatacttcca tagtaactct
     8161 atcgcttctt cagccaaaac ctcgcttacc ggatttactt cttgactcac ataagcctcc
     8221 agagcttgaa ccagtgcatc tataccgctt gtcgcagtga gataaggact cataaaccga
     8281 gtatacagcg ggtcgataat cactaattta gctaaaagat aaggactgcg gaagcttttt
     8341 ttaagcttcg tgcggtaatc agtaagaact gcattggtgg tcatctctga acctgtcccg
     8401 gcagtggtag gaacagctat ccagggaatt cctggtgagg aaagaacttt cccctcaaaa
     8461 taaggcaaca cagtatggga attaccacat aatccagcaa tagctttacc acaatcttga
     8521 gcacttcccc ctcccaggga aatcacaaaa tcacatcgtt ctcgataagc taaatctaaa
     8581 cctctatcta ctgtttctaa agaaggctca ggttctacct ggtcaaagat agaacattgt
     8641 accccggctc cctcaagaga cttaagaatt tcttccaata tcccggtttc ttgcatcgat
     8701 tttttcccgg tcacaagaag tgctttttct ccccatcttt ttactaattc tccagctttt
     8761 ttgccttctc ccccgccaaa ataaatttcg ggaggaagac gaaaaacaaa gttttctttc
     8821 atagtttcct ccatactccc attattcttc ctataatttc tccatcgtct gctgatagag
     8881 atagattgcc atcaattctt ttaatctttc ctccatcgct cttgatgatt aaggctaaac
     8941 cttcttccct tatttcctct tcttttctaa ttaaaagcca gtctcctttc agaatatgcc
     9001 aatcttgcca gttatttttt tctacttgcc agaagaaaaa atctccttct cctaaaaaat
     9061 tgcggggtaa agaaacagga cctcgagaaa gactgtttaa aaagggaaca tccactaaac
     9121 catcttccct atcagggaaa cacagttcta tagttcgggc cttaaaacga tttcttctta
     9181 tcattccttt ctcttccagt ttctcgagat gatagtgaac ggtgcaagaa gataataaac
     9241 ctactccttc tccaatttct ctcaccgtag gaggataacc gttctttcta taataatcct
     9301 gaataaaaag caggatttct tcctgacggg gagaaatttt ctcttttttt ctcatggcta
     9361 tcagggtatt atcgggcagg taagagttct tttcactccg ctcaaggatt gaatttcctt
     9421 gaggatcaat tcaccaattt cccggttatt ttctacctcc actaaagcaa tgaggtcaaa
     9481 aggccccata actctatcta cccgcaaaaa ctgagacaat tgagttaact cctttttgat
     9541 ggcttgtgct ttaccagttt gtacctgaac cagaatataa gcttgaactg acaaaaccaa
     9601 aaccccctat gctacactac ataatatctc gagaggtgag acgaatcact tcatcaatgt
     9661 attgctttaa cacaacagga atcccaggaa tatcgatatt taaaaatccc cgggtaatga
     9721 gggatagaga ttcttcccga gaaagccctc gagagcgtag ataatttact gcttcttctt
     9781 caatgggacc cactgctgcc tcatgagaca gtcgactacg gggagctcca tgcacttcca
     9841 gctccggtat agaaataatt tctgctctct gagaaaatag tattcctttg cattccagat
     9901 gggcttgcgc ttcatcatga taagaaatca gctttcctcg agtataaact ttagaactat
     9961 ctgcagcaca tactcgagaa atcgaattgc ctcgacaccc gcctcccttc aaaagcaaag
    10021 aagaaccaac atcgatgtag gaatctcctt tagcaaaaac aatactctga aaattggcat
    10081 cggcgttttc tccctccatg atagcctgag gaaaagtttg caaagaagag agaggtttaa
    10141 gtaaaacgta ggtattactg tagaaagaat tattacccat ctttactacc gtacgagggc
    10201 gaacatgaaa attctctccc cagttatgaa ccatggtaaa agtgagacga gaattatctc
    10261 ccaagtaaaa ttcactaact ccaatatgca cgccgctttc gttacctcgc actgaagcac
    10321 aaccagtaat caaattagct tctgcccctt cttctaatat taccagatta tgaacactct
    10381 gtattcgaga ctcctcagag aggagaagac aggcctggag aggaacttct attttttgat
    10441 gagcaaaaac ccgaataaaa taaccctcca tgggacagat ttcagaaaaa gcagtgtatt
    10501 tatcagcatc tacaggcact aatccccacc agtactccag aagttcggga tacttctcca
    10561 gagcctcact cactgccata atttccaatt taccctgaaa ggcatcctgt attttctgat
    10621 aaactgccga gcggtctacc tgaagataag ttgctgagct tttgttttct tctaactcca
    10681 ctcccgcata aagggctgct tcccggagag aagcagaaat gctttccacg ctatctactt
    10741 cttcctgaga agaacagaga taaaactcat taaggtctac atcgggacct aaaggtgctt
    10801 ttttatttaa agctttctct gccctttctt ttatttccct caagtaatct tcttttactt
    10861 tcatcgttca cacctcaagc attcctgata acctttctct ctaatatcaa caaaaaggtc
    10921 tcgaggatta ccacggcata caattcgccc ctccataagg acgtgtccca catcagcatt
    10981 caaatattct aaaatatatc cagtgtgggt aattatcagg cccgacctct ttttcgtctt
    11041 tacttgctct ctctccaaaa gctcacttaa agcttttcca atcaaagcaa tgttatcgag
    11101 gtctacccct gattctggct catctatgag agaaagaaga ggacgctgca aaaaaagctg
    11161 aaagagctcc gcccttttag cttcccctcc cgaaaaaccc aaaccaatgc tgcgctccag
    11221 aagataggta cagttaagtt tttccgctac ttcttctata agagaagaac ccccattaac
    11281 tattatctcc cctaaggtcc taagcttaac cccgggaatg acaggcattt tttgaaaagc
    11341 catacctatc cccatcttag ccctctcttc cacggttaaa gggagaatgc tttccccttg
    11401 aaaaagtata tctcctctcg ttacctcata tccaggcatc cccataatag ccattaaaag
    11461 agtagtttta cccgctccgt taggaccgag caaaatgtgg acctcgcccg gtccaatctc
    11521 cagatttaca tcctggagga cttcttctcc tcctactcta actcctaact ccttaatttc
    11581 taacatatca tcaccttcgc tataattata acactttatt actccaggct aaatcatcac
    11641 cacctgtgct ctttcaaagc cattaaaatt ggtagctgaa gcccaggaat aagctccaat
    11701 atcaggaacc acaatcagat cttccagctc taagggaggt agtaaaacat tctctgccac
    11761 cacatctaaa gaatcacaag taggaccggc cagaacagct ggcaactctt cctttgattc
    11821 tcgcaaaggt aagaaacgaa aagcggcttt atcaaaagga atggcagaaa aagttccgta
    11881 tattccatca tccagatagt accacgtaat cccggagcgt accgcttttc caattacctt
    11941 ggttatcaga aaagctgcac tacccactat tactctcccc ggttcagcta taactcgata
    12001 accatctttc aaaaactctt gaagatgggg attaatggcc ttgctaaatg cttccaggct
    12061 tccttctcct tcttcctcat agaggctaag aggaaaacct ccccctatat ctatgacaga
    12121 aatagagatt tctcccgaaa ctttttctac aatttccctg actttttgca gagaataaag
    12181 ataattatta acatttgagc agggagaacc cacatggaaa cttatacctg ctggcttaag
    12241 tcccatgttt tttgcttgaa ggagtagttc tggagccata tccggctcgg caccaaattt
    12301 gtaggaaaga ttggctcgag acccgttaga aggagttttt atcctgacca ataactgggc
    12361 ttcaggataa accatggcaa tttttttcag ttcttcctca ttatcgtatg tcataaggtt
    12421 tacccctatt tgccgagcat agcgcagacc ctttttcctt ttgatggtat tagcaaaaat
    12481 cattctctcc gggggaactc cttggcttga aaccagctct atttcctgga aggaagccac
    12541 atcaaaagaa gaccctatct ctttcaaggt tctgattatg aaaggatgag gattagcttt
    12601 tacagcatag aaaatttcta tgcccggaaa aaattcttga aaagcacggt aattctgcaa
    12661 caatttttcc tcgctgacta caaaaagagg agtatcaaac tgcttcacca gagaaataat
    12721 ttcttcttcg ctaactggca tagctctctc ccaaacttga ttagttctgt ccattaacag
    12781 gcgtcaccca ccatgattta tatgtcacgc ttactttttt acttttcagc gtccgtgtgc
    12841 agtataatag ctcaaagcag tttttgcaag aatcaaactt tatttttgtg gctattttat
    12901 gatagcgtta tagctacggg ggaaaagcct ttgtagctct cctaaacaca aaattatgca
    12961 gatgagagca agaggtcaat tcccttagag aatatatttt ctatagcgag ggtacagaga
    13021 acgatgtaaa aatataataa aggaagtagc atgatgcaga agttgctggt atcaactctc
    13081 ccgaattgtg aaagaaaatt caggagagtt aagggatatg ctttgattcc agaaatgctc
    13141 aagaaatatc gaggcagaag aaaatataga aactgccatg ttagtgatat agagttttat
    13201 aagagctgcc aaaatatttt taactgaaaa cttgacaggc tctaaactta taattcctga
    13261 cagtcatgtt ttgtatttcc actctttgca ctctggagat atactgttaa agtattattg
    13321 caagatattg aagccttata tcgtttatca atattttagg aaggagaaga agggcatgga
    13381 tttgagagtt ttatctcgag aggacatttt ctcaccccgg ccagaatggc gttttgaacc
    13441 agaagctccc gattattttg ccatgtattc aagtctttgg ggaggaattg ttaaagaccc
    13501 cgctttaatg cttattccct tagatgacca catggtccac cggggagatg gaatatttga
    13561 agctttcaag tgtgttaagg gacatattta taattttgac gctcacttat cccgattaga
    13621 aaattctgcc cggttaattt ctctaaactt gcctttcgat ctggaaacta ttaaagaaat
    13681 cactttaaaa acggtagcta ttgcaggagt gaaagattgc agaatacgca tctatgtttc
    13741 ccggggtctg ggaaatgcat ttagttgtag cccggtagat tgtagaagcc agctctatgt
    13801 tgtggtatta aaagaaagcg ttcctccacc ttattattat accgacggag catcggcaat
    13861 aacgagttgc atacctacca aagcctcctt ttttgctcaa gtgaagtctt gcaattatct
    13921 ccccaatgcc ttagctcact tagaagctca tgaaaaggga gcagattttg cgattatgct
    13981 cgaccagcaa ggctacttag cagaaggacc gactcaaagt atagccatag tggaccatca
    14041 aaaaatattt aaatacccca gactggataa taccctgaaa ggtactactt tactccgggc
    14101 agtggcactc gctgaagagc tggttgaggc tggagaatta aaatcaattt ccagcagcga
    14161 tatctccagg gaagaagcct acaatagcct ggagatgttc gcttttactg tgggaattgc
    14221 tgtagtgcca atagtaaaat atgatgagaa agtcatcggt actggaaaac cggggccagt
    14281 atttcgtcgt ctcttgaagc tatttcaaag agattataca gaaaataggg agcttttgac
    14341 ccccgttcct tacgatgaag tagctaatgg ttagacgata attatcacaa aatcccggta
    14401 ttagctatgg ctttcgaacc cttagaatgc tacttttcga tagaattaat aattaagata
    14461 tattaaaaaa aaataaaact actgacacat aaggcaccga gaaatcactt cataaccatc
    14521 ggcctcttct atgttatgag gtagtatcaa acgaggatgg atggtaataa attcaggaac
    14581 aggcttattt gcacaaaaac aatctatcaa atttgctatt tcctgggcca gttgagaggg
    14641 cttaaaatcc accactgcct ctatctttcc ctgaagcaga gcaaaaagca tctccggaga
    14701 agcctccaat accacaaggg gtggaggaga ttcgacctca gaaaatttat caatcaactg
    14761 cctccctgct tcagcatttc ctgctattat taaagatttt tctctgttgg aaatcaactg
    14821 cttgagtgta tctttatcat tttctcctat ctctctgaaa cggggataaa actcttcatt
    14881 tagcccctgc ctcaggcgtg gagcaatttt acccactccc cagatagtga agtggtcatc
    14941 cagaaaaagc ttttctacta cctctcttcg gaaaacttct attacctctt ctacctccac
    15001 ccttatcttg caagaaggga accccaaagg aggcgcaact ccttcccaga aaagaagcaa
    15061 aggttcttta tcttgaggga tttgtgacag aatagaagaa aaatattttt cctcacccaa
    15121 aaacactata agaaaagcct tttctggggg aaagcccaga gatagctctt ctaaagagca
    15181 aagaggaatt aaatgcaccc gagaagggga aaggagcctt cttaaatgaa cttctaaaaa
    15241 agtagaaaaa taatcgagag aagaagaaag aagcaatact tgaggagcct cctgccccgg
    15301 aagcgggctt ccccctccca gaattatgag aataactaat aataaaaaat aacgtctcaa
    15361 atttttcttc ggcgaatgag aaattggtta agcagaacac tgcctatcaa aactaatcct
    15421 aaagctccca gttgccagat ctgatgaata ccagcgagtt gtaaaccatt attgagcata
    15481 gttatgatag ccacactcaa gagcactcct cctactgttc cttctcctcc ttgaatacta
    15541 atacctccca aaactgcaat agtaatagcc tgcaattcat aacctgttcc gatatccggt
    15601 cgagcactgg caatacgaga agtaattacc caggcagcta aagcactcag taacccagaa
    15661 agagcataaa cagtaagccg tactctactt acatcaattc cagaaaaagt tgccgctgtc
    15721 tcattgcttc ccacaccata aagttttctt ccccaggctg tgcgggaaag caaaaagtgg
    15781 agaatcagaa ggacgggaac aaaaatgaag agaacctgga aaggtattcc ccctattcta
    15841 tactgtccta tgaagtaaaa agacttagga aaattggaaa ctggcatagg gttaggccca
    15901 aactgggggt ctacagcaat gattagagcc agagaacgat aaatatacat accagctaag
    15961 gtaactataa gaggaagtat cttgcccacg ctaactaata acccattaaa taaacctgca
    16021 gctaaaccgg ttaacatacc aatcaccaca gccagagaag gagacaatcc tactctacca
    16081 ataagaactc caataactat agcta
//
LOCUS       DAOPSS010000026        15856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_3114_length_15856_cov_10.524524, whole genome shotgun
            sequence.
ACCESSION   DAOPSS010000026 DAOPSS010000000
VERSION     DAOPSS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 15856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15856
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3114_length_15856_cov_10.524524"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2192)
                     /gene="uvrA"
                     /locus_tag="PKN31_02925"
     CDS             complement(<1..2192)
                     /gene="uvrA"
                     /locus_tag="PKN31_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOA98916.1"
                     /translation="MNLNGKIVVKGAREHNLKNVDLEIPRNHFIVITGLSGSGKSSLA
                     FDTIYAEGQRRYLESLSSYARQFLERMEKPDVDYIEGLSPAIAINQKAASRNPRSTVG
                     TVTEVYDYMRVLFARCGKAFCPECGKPITRQTVTQITDEILSLPSGTRVMILAPLVRG
                     RKGEYRKLLGDMLKNGFARVIVNGEMIELEDWENVELDRNKKHEIDLVVDRIVVEEDA
                     ARRISDSVEIALRYGKGVVKMRIKEGEEDRDVLFSEHFACVDCGVSFPEITPRMFSFN
                     NPYGACPACSGLGFLSFVDPDLVVPDRSKSLREGAVEPWGELDSDSYSGERIIKFLKS
                     HGISLNKPFQKLKEEEKDLIFYGDGGFEGVINYLERKLRSATGWWEEEEISPYLSTRT
                     CPTCQGDRLRKESLSVKIQNLSIGDLSRLTIAELRSFFSQLEFSGSELIIAQPIMREI
                     LSRLKFLEEVGLDYLTLNRPAATLSGGEAQRIRLATQIGSGLVGVLYVLDEPTIGLHP
                     RDNQKLISTLKNLRDLGNTVLVVEHDADTIRNADFIVDMGPGAGERGGEVIAQGSLLE
                     IMNDPHSITGLYLSGKREVPLPRERKKPGEEWLIVKGARAHNLKNITVEIPLGLLVGI
                     TGVSGSGKSTLMDDVLYRSLARTFYRSRWEAGEHDSIEGVEFLDKVVVIDQSPIGRTP
                     RSNPATYTGVFTEIRNLFTKLPESRARGYRPGRFSFNVKGGRCEACQG"
     gene            complement(2189..4219)
                     /gene="uvrB"
                     /locus_tag="PKN31_02930"
     CDS             complement(2189..4219)
                     /gene="uvrB"
                     /locus_tag="PKN31_02930"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOA98917.1"
                     /translation="MKKDSFHLSSLYEPCGDQPQAIEQLVDGLKKGYRYQTLLGVTGS
                     GKTFTMANVIAQINLPTIIISHNKTLAAQLYSEMRAFFPDNAVEYFVSYYDYYQPEAY
                     VSEYDLYIEKDASINQQLDRLRLKATSSLMERRDVVIVASVSCIYGIGSPREYEKRIF
                     HLRVGQEWKREDLVRRFIDLLYERNEIEFSPGYFRMKGDTVEIYPAYSETALRLEFFG
                     PELECIKVFEPVSGKTLERLEEVFIYPAKHYLVDESEFERALQNIEREMEERVNFFLS
                     AGRLVEADRLRRRTKYDLELLRETGYCPGIENYSRHLDGRKPGEPPYTLLDYFPSDYL
                     VIIDESHVTIPQLRGMQEGDRSRKKSLVDFGFRLPSAFDNRPLTFEEFESKVKRCIFV
                     SATPSAFELEHSEQVVEQLIRPTGLLDPQAEIRKARGQVEDLLGEIRKVVKKNQRVLV
                     TTLTKKSAEDLCDFLYGMGIKVRYLHSEIDTLERAKIVRDLRRGEFDVLVGVNLLREG
                     LDLPEVALVAILDADKEGFLRSEVSLIQTMGRAARNVEGRVILYADEMTGSLKRAVEE
                     TRRRRKVQEEYNREHGIIPQSIVKEVRTLLPEEGAVSKEAEEVVELVEGLKEKGETKE
                     KWIEKLTQEMQEAARRLEFEKAAILRDEILKIKSGNRIIKEKRKELKVGNKT"
     gene            complement(4276..4863)
                     /gene="coaE"
                     /locus_tag="PKN31_02935"
     CDS             complement(4276..4863)
                     /gene="coaE"
                     /locus_tag="PKN31_02935"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888955.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOA98918.1"
                     /translation="MSPVWAIGGGTAAGKSTVARILREKGIEVWELDKLSRELSKKGG
                     PLWKSIVKTFGPQFLTEKGELDRTKLGKMVFRDWENLFNLNSVTHPVLLQEVRKKLAS
                     VPPSRIVAIEGAVLFEAGFEPLWDKLIFVEADEAIREKRLLKTTGLKAREIRDRLRLQ
                     RFLNCLKRRADFILKNEGSLEELEETIEDWLEELK"
     gene            complement(4873..6297)
                     /locus_tag="PKN31_02940"
     CDS             complement(4873..6297)
                     /locus_tag="PKN31_02940"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOA98919.1"
                     /translation="MEEVILVATEAYPYAKSGGMGDVIGALFKYLPDYDFSVTLFLPA
                     YRGVLDNYSFSLEREVDFYFGDAAVTAQFFKMEEGKQKIIAVQGKDFFSRERMYGYPD
                     DLERFIFFSRAVFEYLVRWQEGNFLVHAHDWQSALLCAYIVKYWPSYRTKPEKIVFTI
                     HNLAYQGIGRGDLFRLINLPGQFFTHEYLEFYGNLNLMKAGLIFSDVITTVSPTYARE
                     ICSPEYGEGLDGVLRAISQKKKIVGIVNGIDREVFNPATDSLIPANYDLSSREKKAIN
                     KEKLIRQFFPKAKDFSLPVISFVSRLVEQKGIDLLLHDPAHFFNLPAYWFFLGTGEEI
                     YEKALKDMEERYPKVKAAIRFDDELSHLVYAASDLLLLPSRFEPCGISQMIAMNYGTL
                     PLVRRVGGLIDTVIDYPFNPCLSTGFQFENYSFSDLSKALTRALNTYFENQELWNTMV
                     INAMQSDFSWENSIPRYLEIYRER"
     gene            complement(6324..7235)
                     /locus_tag="PKN31_02945"
     CDS             complement(6324..7235)
                     /locus_tag="PKN31_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOA98920.1"
                     /translation="MEDKKLVVIDGSSLIYRAFYAMPHLTTSSGLPTGAALGFLNMVL
                     KLLDDYRPFNMVVVFDHPQKTFRHELQESYKAQREAMPDELKPQIETVKELLELMGFV
                     ILEMPGWEGDDLMGTVKEKAKPDFQVLLVTSDLDMLQLLDERTVLLQPHRGVTNLKKI
                     DLEEFEKEWGISPEQWIDVLALSGDPSDNIAGVPGIGEKTAVKLIRQFGSWEGILKNL
                     SLLPPKLREAIESNRDKVEENRKLVALERDLPLDLEIKAWGWSKVDKNKLFQKLDALE
                     FRKVKERLLKNRVNSGSLFSRSSEEKE"
     gene            7292..7379
                     /locus_tag="PKN31_02950"
     tRNA            7292..7379
                     /locus_tag="PKN31_02950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7326..7328,aa:Leu,seq:caa)
     gene            complement(7382..8218)
                     /locus_tag="PKN31_02955"
     CDS             complement(7382..8218)
                     /locus_tag="PKN31_02955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA98921.1"
                     /translation="MKNKIGKLIIIVWLVLGIAPGLLAQTKEECYQNGYIYFSQGKYE
                     EAREWYRKALEIDPNFWEARYWLGKTEEQLGRIEEALKEWVVVLMAQPDNRDVFQKWR
                     YYAPSYVSLQESDIQRLKSIFLEGGTPSFSEEEAWQELMPKAFLLLGRKDFTSPYLGA
                     RIMRWGGRNLSSLFYSYERRGYEQALDNLLLNLDEDQELVYQFIKECEERFGEDENFR
                     EKLSLLYEKIFAQQLNLSGEQAEGIKKVELEVEGREVKVSATLSEEDALLHPDTGFYL
                     GE"
     gene            complement(8215..11625)
                     /locus_tag="PKN31_02960"
     CDS             complement(8215..11625)
                     /locus_tag="PKN31_02960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOA98922.1"
                     /translation="MSFSHLHVHTEYSLLDGAIRIDDLINKVKSQGMEAVAITDHGVM
                     YGVVDFYARAKKAGIKPIIGCEVYVAPGSRFEKTVVRGTESAYHLVLLAENQEGYRNL
                     VQLVSKAFLEGFYYKPRIDKALLREHREGLIGLSACLAGEIPSLILNGEREKAEEVAL
                     EYAEILGKDNFFLEIQENKIPEQKIVNEALLEIGSKYGIEIVATNDCHYLEAEDASVH
                     DVLLAIQTATTLDDPKRLRFPTTEFYLKSPEEMERDFSYYPRALENASLIAERCQVDL
                     ELGKVNLPDFPVPPGFDAPGYLRSLCLQGLARRYGEPPPERAREQLEYELKVIEDMGY
                     STYFLIVWDFVNFARQKGIVVGPGRGSAAGSIVAYTLGITSIDPLQYGLLFERFLNPE
                     RVSMPDIDIDFCFERRGEVIEYVEEKYGKTNVAQIITFGRMMARQAVRDVGRALGWSY
                     GEVDKIAKLIPGGPGVTLKKALESNSQLRELSKQNKELQHLLEIASRIEGMCRHASIH
                     AAGVVISNQPLTDYVPLQRMGEGEIVTQFEMDTLQELGLLKMDFLGLRTLTVIEKTLQ
                     IIKNTRHQEIDLEKIPLNDEATFSLLSRGETTGVFQLESSGMKELLKRLNPSDIEDLI
                     AILALYRPGPLGSGMIEDFINRKHGKVKIDYPHPALAAILRETYGVIVYQEQVMRIAA
                     ELAGFTLGEADILRRAMGKKQPEVMKEQRAKFVEGALKRGCSKEKAEEIFDLIEYFAG
                     YGFNKSHSAAYALISYQTAYLKAHYPTEYLAAYLTSVANDNEKVAKFIGECKRLGIQV
                     LLPDINESLANFTVVGENKIRFGLAAIKNVGDIAVEEILTRRREGKFLSIFDFVERVD
                     SRVINKRVLESLVQAGAFDELHPHRAQLLASLEDISLLSRKKKSKKKQKSLFGEENNS
                     GESFSLREAEELPREELLRMEKEMLGLYISDHPLKLESISSSWSAVPLERISNFSQEV
                     RVRVRGVVSKSKKITTKNGGNMYFVTLEDETGSVEVVFFPRLASQLEDYLSHKQVIWV
                     VGKVDLLDNGERKIIAEELIDIEEKKNNISTVHIAIPEKEDTLKTVYFLKDYLRRFPG
                     SFPVILHIRGEKENWAVELGKSFCIDWNEKIEKTIKNSLEEGEVWLAG"
     gene            complement(11628..13073)
                     /gene="gatB"
                     /locus_tag="PKN31_02965"
     CDS             complement(11628..13073)
                     /gene="gatB"
                     /locus_tag="PKN31_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOA98923.1"
                     /translation="MSDFTTTIGLEVHCQLLTQAKMFCHCSTDYIGKEPNTLVCPVCL
                     GLPGSLPVLNQKVVELALRAALALHCQILPQGVFHRKNYFYPDLPKGYQISQYDLPIG
                     VDGFLEFYSRGVKKKVRIKRVHIEEDAGKLIHEGVFLPPGTSGVDCNRCGIPLAEVVT
                     EPELSSPEEARDCLMMLRNIVCYLGVSDGNMEEGSLRCDANISLSSSSLLGVKVEVKN
                     MNSFRSVYRALSYEVERQRKLLEEGKPIFQETRHWDEVKGVTVSARGKEGAEDYRYFP
                     DPDLLPFSIKGEMIEEIKSELPELPLQREERFMTEYELSQGEAEILVQDKEIADFFED
                     TLRDFNEPRMVCNWITTEVFKNLKELGIDISQASITPAHLAELLKMVDKKEISGTIAK
                     SVLEEMFATGARAREVITRRGISFISDEKELENIVREVIEKNPQSVADYLSGKEKALQ
                     FLIGQVMKETRGQADPELTKSILIKVLSSEE"
     gene            complement(13066..14544)
                     /gene="gatA"
                     /locus_tag="PKN31_02970"
     CDS             complement(13066..14544)
                     /gene="gatA"
                     /locus_tag="PKN31_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOA98924.1"
                     /translation="MKIEEFRDFSIGDYHRLFKDKEISPLELVNLFLERIKEGEDKIH
                     AFLFVNEEKAKDEAQKKAENLPDELPPLWGLPVAIKDNICTRGIPTTCGSKMLENFTP
                     PYDATVVERLKKAGAIILGKTNMDEFAMGSSTQTSAFGPTYNPFDRRRVPGGSSGGSA
                     ASVCSLEAPFALGSDTGGSVRQPAAFCGVVGLRPTYGRISRYGVVSFASSLDQVGLIT
                     RKVEDAITLLEVVSGKDDKDSTCAPYPPFGRSEIPASEEIRKLKVGVPREYFPSGLEK
                     VIKTKIQEVLEKLKEEEIEIVEVSLPHTSYALESYYIVAAAEASSNLARYDGVVYGLR
                     VEGENLQDMYFKTRTLGFGREVKKRIILGTYALSSGYYDEYYLKGMKVRTLVRQDFEE
                     AFGKCDLLITPTTPSLPFFPEKKGRDPYEMYLNDMFTVPSAMAGLPSLSLNCGYDEGL
                     PVGLQIIGRPFEEGKLLGLALFLEKLLALPSPFPLFKEDNNE"
     gene            complement(14525..14851)
                     /gene="gatC"
                     /locus_tag="PKN31_02975"
     CDS             complement(14525..14851)
                     /gene="gatC"
                     /locus_tag="PKN31_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOA98925.1"
                     /translation="MREVISLQELKRIAQVSKLELSEEELQGFFEDINNVVSRFEKLN
                     DLDLQDISPTSHLSWSEPPFSSDEPRAGEGGEEVLKGAPQVANGYFVVPRVVDKEEKE
                     NEDRRV"
     gene            complement(14868..>15856)
                     /gene="ligA"
                     /locus_tag="PKN31_02980"
     CDS             complement(14868..>15856)
                     /gene="ligA"
                     /locus_tag="PKN31_02980"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOA98926.1"
                     /translation="PVAILEPVNVGGAIVKRATLHNEDEIKRKDVRIGDWVMVTRAGE
                     VIPEITEVLKERRTGEERVFAMPEECPVCHSRVVREEGEVAWRCINASCPAQIKERIR
                     HWASRDAMDIEGLGEKLIDRLVEGGWVKTIPDLYRLNKETLLQLERMGNKSADNLLRA
                     IEKSKKRELPRFIYALGIPHVGQFIARLLAEHFGSLDRLKEAKEEELLTIEGIGPTVA
                     DSVRQFFSNPQNRQMLEELKSLGVEPEELKRREEGTLAGKTFVFTGALSKLSRDKARA
                     LVEERGGKVADTVSHRVDYVVVGENPGSKLAMARERGIKILTEEEFYQLLGI"
BASE COUNT         4311 a         4115 c         2824 g         4606 t
ORIGIN      
        1 ttaccctgac aggcttcaca ccttcctcct ttaacgttaa aactaaagcg tcccggacga
       61 taacctcgag cccgggattc aggaagcttg gtaaagagat tcctgatttc tgtgaaaact
      121 ccggtgtagg tagcagggtt agaacgagga gttctaccaa tgggagactg atcaataacc
      181 accactttgt ccagaaattc caccccttct atagaatcgt gttcccctgc ttcccaacgg
      241 gaacgataaa aagtccgggc taaagaccga tacaaaacat catccatcaa ggtgcttttt
      301 cccgaaccag agacgcccgt tatccccacc agaagcccca gaggaatttc cacggtgata
      361 tttttcaaat tatgggctcg cgctcctttg actatcagcc attcctctcc cggtttcttt
      421 ctttctcgag gaagaggcac ttctcttttt ccgctcaaat acagacccgt tatactatga
      481 gggtcgttca ttatctctaa aagggagccc tgggctatta cttctccccc tctttctcct
      541 gcccctggtc ccatatccac gataaaatca gcatttctga tggtatcggc atcatgttcc
      601 accactaaaa ctgtattgcc taaatcccgc aaatttttta gagtagaaat cagcttctgg
      661 ttatctcgag gatgcaggcc aatggtaggc tcatctaaaa catagagaac tcccaccaga
      721 cccgagccaa tttgagtagc caaacggatt cgctgcgctt ctcctcctga aagagtggca
      781 gcggggcgat taagcgtgag ataatcaaga cccacttctt ctaagaattt caaacgagaa
      841 agaatctccc gcattatggg ttgagcaatt atcagttcgc tcccggaaaa ttccagttga
      901 gaaaaaaagg agcgtaactc agcaatagtc aggcgagaaa ggtctccaat ggagagattc
      961 tggattttca ccgacaaact ctcttttcgc aggcggtctc cctggcaggt cggacaggtt
     1021 ctcgtagaca gataaggaga tatttcttct tcctcccacc atcctgtagc agaacgcagt
     1081 tttctctcta aataattgat gactccctca aaacctccat caccatagaa aattaagtct
     1141 ttctcttctt ccttcagttt ttggaagggt ttattcaagc ttatcccgtg agatttcaaa
     1201 aactttataa ttctctctcc actataacta tcagagtcta actctcccca gggttctacc
     1261 gctccttccc tcaggctttt cgaccgatcg ggaactacaa ggtcagggtc aacaaaactt
     1321 aaaaatccca gcccactaca ggccggacat gctccataag gattattgaa agaaaacatt
     1381 cggggagtga tttcaggaaa actaactccg cagtccacac aggcaaaatg ctcgctaaag
     1441 aggacgtctc tatcttcttc cccttcttta attctcatct ttactactcc cttaccgtag
     1501 cggagggcta tctccactga atcacttatc ctccttgccg catcttcttc taccactatc
     1561 cggtccacca ctaaatcaat ttcatgtttt ttatttctgt ctaactctac attttcccag
     1621 tcttccagct ctatcatttc cccgtttaca atcacccgag caaaaccatt tttcagcata
     1681 tctccgagga gcttgcgata ttcccctttt cttccccgca ctaaaggtgc taaaatcatc
     1741 acccgcgtgc cagatggcag agataagatt tcatcagtta tctgagtaac agtctggcgt
     1801 gtgatgggct ttccacactc cggacaaaaa gccttcccgc agcgagcaaa caagactcgc
     1861 atataatcgt aaacctcagt aactgtcccc actgtagagc gggggttgcg agaagcagct
     1921 ttttgattta tagcaatagc tggagataag ccttcgatgt agtccacatc gggcttttcc
     1981 attctctcca aaaactgtcg agcataagag gaaagagact ccagatacct tctctgtcct
     2041 tcagcataaa tggtgtcgaa agccagagaa gacttccccg accccgataa accagtaata
     2101 actataaagt gattacgagg aatttctaaa tccacatttt taagattgtg ttcccgtgct
     2161 cctttaacta cgattttccc gtttagattc atgttttatt cccaactttc aattctttcc
     2221 ttttttcttt gattatccgg ttaccagatt ttattttgag aatttcatct ctcaaaatag
     2281 ctgctttttc aaactctaag cgacgcgctg cctcttgcat ctcctgagtc agtttctcta
     2341 tccacttctc tttagtttct cctttttctt tcaacccttc cactaattcc accacctctt
     2401 ctgcttcctt agaaacagct ccttcttcag gaagaagagt acgcacctct ttaactatac
     2461 tctgaggtat tattccgtgt tcccggttgt attcttcctg aaccttccgc cggcgtctgg
     2521 tttcctctac cgctcttttt aaagaaccgg tcatctcatc agcataaaga ataactctac
     2581 cttctacatt gcgagctgcc cgacccatag tttgaataag agacacttcc gagcgcaaaa
     2641 acccttcttt atcggcatcc aggatagcta ccagtgccac ctccggtagg tctaaccctt
     2701 ctcgcaacag gttaactccc accaggacgt caaactctcc tctcctcaaa tccctgacaa
     2761 tttttgctct ttctaaagta tcaatttcgg agtgaagata gcgcactttt attcccatcc
     2821 cgtagagaaa atcgcacagg tcttcagcgc tctttttggt aagagtagtt accagaaccc
     2881 tctgattttt ctttaccact tttcggattt ctcctaataa gtcttccacc tgccctcgcg
     2941 cttttcttat ttctgcctga gggtctaaaa gaccggtagg tctgatcaat tgctctacta
     3001 cctgttcact atgctccagc tcaaaagcag aaggagtagc agaaacaaaa atacacctct
     3061 tcactttgct ttcaaattcc tcaaaagtaa gaggacgatt gtcaaaagca gaaggaagcc
     3121 gaaaaccaaa atctaccaga gatttcttcc gggagcggtc tccttcctgc atgcccctca
     3181 attgaggaat ggtaacgtga gattcatcta ttataaccag ataatcagag ggaaaataat
     3241 cgagcaaggt atagggaggc tctcccggct ttctcccatc taaatgacgg gagtagtttt
     3301 ctatccccgg acagtaaccg gtctcccgca aaagctccag gtcatattta gtccttcttc
     3361 tcaatctatc cgcctccacc aggcgtcctg cagagaggaa aaagtttacc ctctcttcca
     3421 tctctctttc aatattctgg agagctcgct cgaattcact ttcatctacc agataatgtt
     3481 tggcagggta aatgaacact tcttccagtc gctccagagt tttaccactc accggttcaa
     3541 atacttttat acactccagc tccggaccaa aaaattccag gcggagggca gtttcgctat
     3601 aagcaggata tatctctact gtatctcctt tcatccggaa atacccgggg ctgaattcaa
     3661 tctcatttct ttcgtaaagc aagtctatga aacgtctgac taaatcttcc cttttccatt
     3721 cctgccctac cctcaagtga aaaattctct tctcatattc ccggggagaa ccaatgccat
     3781 aaatacagga aaccgaagcc actattacca catctcttct ctccataaga gaagaggtag
     3841 ctttcaaccg caggcggtct aattgctggt taatggaagc atctttttcg atatagaggt
     3901 catattcaga gacataagcc tccggttgat aataatcgta atagctgaca aaatattcta
     3961 cagcattatc aggaaaaaag gctctcatct cgctgtataa ttgagcagct aaagttttat
     4021 tatgagagat aatgatggtg ggaagattga tttgagcgat gacattagcc atggtaaagg
     4081 tcttacccga gccggtaaca cccagtaaag tctggtaccg atatcctttc ttcaagccat
     4141 ccaccagttg ttcaatagcc tgaggttgat ccccgcaggg ctcataaaga gaagaaagat
     4201 gaaaactatc ttttttcatt ggccttactg ctccttgcta ttttttcctc caataaaaac
     4261 ccatcagagg attttctatt tcagctcctc cagccaatct tcgattgttt cctccagctc
     4321 ctctaaagaa ccctcatttt tcaggataaa atctgctctt cttttcaagc aatttaagaa
     4381 tctctgtaag cgcagacggt ctcttatttc tctcgctttt agtcctgtgg ttttcaataa
     4441 cctcttttcc cttattgcct catccgcctc tacaaatatc aatttatccc agagaggctc
     4501 aaaaccagcc tcaaaaagga cagctccctc aattgctact atccgggaag gaggaactga
     4561 agctaacttt ttacgcactt cctggagaag aacaggatgg gtaacgctat tgagattaaa
     4621 aaggttttcc cagtcccgga aaaccatttt tcccagttta gtgcggtcaa gctctccctt
     4681 ttcggtcaaa aactgcggcc caaaagtttt tactatgctc ttccacagag gccctccctt
     4741 tttagaaagc tccctgctca atttatccag ctcccatact tctattcctt tttcccggag
     4801 gatacgggct acagtgcttt tgcctgccgc agttcctcct cctatagccc acactggaga
     4861 catgaatttc tcctaccttt ctcgataaat ttctaaataa cggggaatgg aattttccca
     4921 ggaaaaatca gattgcatag cattaatcac catggtattc cacagttctt gattttcaaa
     4981 ataagtgttc agagcccgag tgagtgcttt agataaatca gagaaggaat agttttcaaa
     5041 ctgaaagcca gtgctgagac aaggattaaa aggatagtca atcacagtgt caatcaagcc
     5101 ccccacccga cgcacgagag gtagtgttcc ataattcatg gcaatcatct ggcttattcc
     5161 gcagggctca aaccgggaag ggagaagcag caaatcagaa gcagcataca ctaaatggga
     5221 taattcatca tcaaacctga tagccgcctt tacttttggg tatctctctt ccatgtcttt
     5281 taaagctttc tcataaatct cctcacctgt tcccaggaaa aaccagtaag ctggtaaatt
     5341 aaaaaagtga gccgggtcgt gcaaaagaag gtctatccct ttttgttcta ctaaacgaga
     5401 gacaaaagaa atcaccggta gagaaaagtc ctttgccttc gggaaaaact gccgaatcaa
     5461 tttttctttg tttattgctt tcttttctcg ggaggataaa tcatagtttg ccggaatcaa
     5521 agaatcagtg gccggattaa agacttctct atctatgcca tttacaatac ccactatttt
     5581 ctttttctgg gaaatagctc gtaaaacccc atccaatcct tctccatact cgggagagca
     5641 tatctctcga gcataagtgg ggctgactgt ggttataaca tcagaaaata tcaagcctgc
     5701 tttcatcaaa ttcaaattac cataaaattc cagatattca tgagtaaaaa actgaccggg
     5761 aagattaatc aaacggaaaa ggtcacctcg accaatgccc tgataagcca gattatgaat
     5821 ggtaaatact attttttctg gcttagtacg gtatgaaggc cagtatttta ctatgtaggc
     5881 acaaagtaga gcgctctgcc agtcgtgagc atgaactaag aaattccctt cctgccatct
     5941 caccagatac tcaaaaaccg cccgggaaaa gaatatgaac ctttctaaat catcgggata
     6001 accatacatc ctctcccgag agaaaaaatc tttcccctgt acagctataa ttttttgttt
     6061 tccttcctcc attttgaaaa attgagcagt cactgccgca tcgccaaagt aaaaatccac
     6121 ctctcgctcc agagaaaagg aataattatc cagcactcct cgataggcag gaagaaaaag
     6181 agtaacagaa aaatcgtaat cgggcagata cttaaaaagc gctccaatta catctcccat
     6241 tcctccactt ttggcataag gataggcttc agtggcaacc agtattactt cttccatatt
     6301 catcctccct tataattaat atatcactct ttttcttcgc tcgagcggga aaaaagagaa
     6361 ccgctattca ctcgattttt caaaaggcgt tctttaactt tccgaaactc caaagcatcc
     6421 agcttctgga acaatttgtt tttatccact tttgaccatc cccaggcttt tatctctaaa
     6481 tctaagggga ggtctctctc cagggcaacc aattttctat tttcctccac tttatcccgg
     6541 ttgctttcga tggcttcccg gagcttagga ggtaaaagag agaggttctt tagaattcct
     6601 tcccagctac caaattgtct gattagtttt actgccgttt tttctcctat ccccggaaca
     6661 ccagcaatgt tatctgaggg gtctccgctc aaagctaaaa catcaatcca ctgctcgggg
     6721 gagatacccc actctttttc aaattcttct aaatctattt tcttcaaatt agtaactcct
     6781 cggtggggtt gaagaagtac ggttctctca tccagaagtt gcagcatatc taaatcagaa
     6841 gtaaccagta aaacctgaaa gtcgggtttt gctttttctt taacagttcc catcaaatca
     6901 tcgccttccc agccgggcat ctctaagata acaaatccca tgagctccag aagttcttta
     6961 accgtctcta tttgcggttt tagctcatcg ggcatagctt cacgctgtgc tttgtagctt
     7021 tcctgtagct catggcgaaa ggtcttttgg ggatggtcaa aaacaaccac catattaaaa
     7081 ggacgataat cgtcaagaag tttcaaaacc atattcaaaa aacccagagc agctccggtg
     7141 ggcaaaccag aagaagtagt taaatggggc atggcataaa aagcacgata aatcaaagaa
     7201 cttccatcta tcactaccaa ttttttatct tccatttcct gacctcaact aattgacaaa
     7261 aagataattt tccttataat tttctgatag agccgaagtg gcggaacagg cagacgcact
     7321 agactcaaaa tctagcgagc tctgcgctcg tgcgggttcg actcccgcct tcggcaccac
     7381 ttcactcccc gagataaaat cctgtgtccg gatgaagtaa tgcatcttct tctgataaag
     7441 tagcactaac tttcacttct cttccttcca cctctaattc aacttttttt attccttccg
     7501 cttgttcccc ggacaaattc agttgttgag caaagatttt ctcatacaga agagaaagct
     7561 tctcccggaa attttcgtct tccccaaaac gctcttcaca ctcttttata aattgataaa
     7621 ccagctcctg gtcctcatcc agattaagaa gcaggttgtc taaagcttgc tcataccctc
     7681 tccgctcata ggagtaaaag agagagctta aatttcttcc tccccacctc attatccgag
     7741 cccccaggta aggggaagta aaatcttttc ttcctaatag cagaaaggcc ttaggcatta
     7801 gttcttgcca ggcttcctct tcgctgaaag aaggagtgcc tccttccaga aatatacttt
     7861 ttaatctctg gatatcactt tcttgcagag agacataaga gggagcataa tagcgccatt
     7921 tttgaaaaac atcccgatta tcaggttgag ccattaaaac cacaacccac tctttaagtg
     7981 cttcttctat cctgcccagt tgctcttcag tttttcccag ccagtatcga gcttcccaga
     8041 aattggggtc tatttccaga gctttcctgt accactctct ggcttcttca tacttacctt
     8101 gagaaaagta aatatagcca ttctggtaac actcctcctt ggtttgagcc aaaagccctg
     8161 gagctattcc caacaccaac cacactatga taatcagttt cccaatttta tttttcatcc
     8221 tgctaaccag acctctccct cctctaaaga attcttaatt gtcttttcta tcttttcatt
     8281 ccaatcaata caaaatgact ttcccagctc taccgcccag ttttcctttt ctccccggat
     8341 atgaagtata accggaaaag aaccgggaaa acggcgaaga tagtctttta aaaagtaaac
     8401 agttttaagg gtatcttctt tctcgggaat tgcaatatgc acggtggaga tgttattttt
     8461 cttttcttct atatctatca gctcttcagc gattatctta cgctctccgt tatccagcag
     8521 gtccactttt ccaactaccc agatcacctg cttgtgagaa agataatctt ctaactggga
     8581 agccagcctg ggaaagaaaa ctacttcaac gctaccagtt tcatcctcca gagtgacaaa
     8641 atacatattg cctccattct tggtggtgat ttttttactt ttgctcacca ctcctctcac
     8701 tctgactctc acttcctggc taaagttaga aatccgctcc agaggaacag cagaccagga
     8761 agaagagata gattccagct tcagaggatg gtcactgata tagagaccca gcatttcttt
     8821 ttccattctc aagagctctt cccggggtag ctcttctgcc tcccggaggg aaaagctttc
     8881 tccgctattg ttttcttccc cgaaaagact tttttgtttt tttttgcttt ttttcttacg
     8941 ggaaagaaga gatatatctt ctaatgaagc caaaagttga gcccgatggg ggtgaagttc
     9001 atcaaatgct cctgcctgca ccaggctttc caaaactctt ttatttatga ctcgagaatc
     9061 cactcgctct acaaaatcaa aaatactaag gaatttcccc tctctgcgtc gggtcaaaat
     9121 ttcctccact gcgatatcgc ccacattttt aatggctgcc agaccaaacc tgatcttatt
     9181 ttctcccact acggtgaagt tggccaaact ctcgtttata tcaggcaaca aaacttgaat
     9241 ccccaatctc ttgcactccc cgatgaattt agccaccttt tcattgtcat tggccacgct
     9301 ggtaagatag gccgctaaat attctgtggg atagtgagct ttcaagtatg ccgtttgata
     9361 ggaaattaaa gcataggcag cgctatgaga tttattgaat ccataaccgg caaaatattc
     9421 gatgaggtca aatatctctt ctgctttttc cttgctgcat cctctcttta gcgctccttc
     9481 cacgaattta gctctctgct ctttcataac ctctggctgt ttcttaccca tcgcccggcg
     9541 caaaatatcc gcttctccta aagtaaaacc ggccagttct gcagcaattc tcataacttg
     9601 ctcctgataa actatcactc cataagtctc tcgcaaaata gctgccagtg ctggatgagg
     9661 gtaatctatt ttcacttttc catgttttcg gttgatgaaa tcttcaatca ttccgctacc
     9721 taaaggaccg gggcgataaa gagctaaaat agctatcagg tcttcaatat cggaagggtt
     9781 tagcctcttt aataattctt tcatcccgct actttccagc tgaaaaactc cagtggtctc
     9841 tccccgcgag agcaaagaaa aagtagcttc atcgttcagg ggaatttttt ccaggtctat
     9901 ttcctggtgc ctggtatttt taattatctg caaggtcttt tctattaccg tcagagtccg
     9961 caaccccaga aagtccatct tcagaagccc cagctcttgc agagtgtcca tttcaaactg
    10021 ggtaactatt tccccctccc ccatcctctg cagaggaaca taatcggtaa gcggctgatt
    10081 agatatcacc accccggcag catgaattga ggcgtgacgg cacatccctt ctattcgaga
    10141 agctatctcc agaagatgct gcagttcttt attctgtttg gataactccc gcagctgaga
    10201 gttagattcc agggcctttt tcagggtaac tcccggacct ccgggaatta gcttggcaat
    10261 cttatccacc tcgccatagc tccagcccag agctcttcct acgtctctta ctgcctgacg
    10321 cgccatcatt cttccaaaag taataatctg tgccacgttg gtttttccat acttctcttc
    10381 tacatattcg ataacttctc cccgccgttc aaaacaaaaa tcgatatcaa tatcgggcat
    10441 gctcacccgc tcaggattca aaaaacgctc aaagagtaag ccatattgca aagggtcaat
    10501 gctcgtaatc cccagagtat aagccactat actaccggcg gctgagcctc tccctgggcc
    10561 caccactatg cccttttgcc gggcaaaatt cacaaaatcc cagacaatca ggaaataggt
    10621 agaatagccc atatcctcaa tcacttttaa ttcatattcc agctgctccc gggcacgctc
    10681 gggaggaggc tctccatatc ttctcgctaa accctgaaga cataaactgc gaaggtatcc
    10741 tggcgcatca aatcctggag gaacaggaaa atcgggaaga ttgactttcc ccaattctaa
    10801 atccacctga catctttctg caatcaaaga agcattttct aaagcccgag gatagtaaga
    10861 aaaatctctc tccatctcct ccggtgactt gagataaaac tctgtagtag ggaaacgaag
    10921 tctcttgggg tcatccagag tggtagcagt ttgaatagct aaaagtacat catgcactga
    10981 agcatcttcc gcttctaagt aatggcagtc attggtagcc actatttcga tgccatattt
    11041 acttcctatc tctaaaagtg cctcgttaac tatcttctgc tcgggaattt tgttttcctg
    11101 aatttccagg aaaaaattat cctttcccag gatttctgca tactccagtg caacttcttc
    11161 tgctttttcc ctttctccgt tcaaaatcaa gctgggaatt tctcctgcaa gacatgccga
    11221 aagaccaatt aagccttccc gatgttctcg aagaagagct ttatcaatac gaggtttata
    11281 gtaaaaacct tccaggaagg ctttgctcac cagctgtact aaatttctat acccttcttg
    11341 attttcagct aaaagaacca ggtgataagc ggactcagtc ccccgcacca ctgttttttc
    11401 aaaccggctt cccggtgcca cataaacctc acaaccaatg atgggcttaa tccctgcttt
    11461 cttagcccgg gcataaaaat ccaccactcc atacattact ccgtggtcgg tgatggctac
    11521 cgcttccatc ccctgggatt ttactttatt tatcaaatca tcaatccgaa tagctccatc
    11581 taaaagactg tattcggtat gaacatgtaa gtgggaaaaa ctcattttca ctcctcactg
    11641 gaaagtactt taatcaaaat acttttggtc aattccgggt ctgcttgtcc ccgagtctct
    11701 ttcattacct gaccgattaa gaactgaaga gctttttctt tgccactcaa gtaatcggct
    11761 acgctctggg gatttttttc gatgacttcc ctaactatgt tctccagttc tttttcatcg
    11821 cttatgaatg aaattccccg ccgggtaatg acttcccgag ctcgagcccc agtagcaaac
    11881 atctcttcta aaacactttt cgctatggtt ccactgattt cttttttatc caccatctta
    11941 agcagctcag ctaaatgtgc aggagtaata cttgcctgag agatgtctat tcccagttct
    12001 ttcaaatttt taaaaacctc cgttgttatc cagttacaaa ccatccgggg ttcattaaaa
    12061 tctcgcaaag tatcttcaaa aaaatcggct atttccttat cctgcaccag gatttctgct
    12121 tctccctggg agagttcata ctcggtcatg aacctctctt ctctctgtaa tggtaactca
    12181 ggaagctccg attttatttc ctcaatcatt tctcctttaa tagaaaaagg taacaaatcc
    12241 gggtcgggga agtagcgata atcttctgct ccttctttcc ctcgagcaga aaccgtcact
    12301 ccttttacct catcccagtg gcgagtttcc tgaaaaatgg gttttccttc ttctaacaat
    12361 ttcctttgcc tctccacttc gtagctcaga gcccggtaaa ccgaacgaaa agaattcata
    12421 ttcttaactt ctaccttaac tcccaaaagt gaagaagaag aaagagaaat attggcatca
    12481 caccgcagtg acccttcttc catattacca tcactcactc ccagatagca aactatgtta
    12541 cgcaacatca tcaaacaatc tcgagcctcc tcgggagaag aaagttcggg ttcagtaacc
    12601 acctccgcaa gagggattcc acatcgatta caatctacac ctgaagtccc aggaggaaga
    12661 aagacgcctt cgtgaatcaa tttaccggca tcttcttcta tatgtaccct ttttattctc
    12721 actttctttt taactccccg gctgtaaaat tccagaaatc cgtccactcc gatgggtaaa
    12781 tcatactgag agatttgata tcctttgggt aaatcaggat aaaaataatt tttacggtga
    12841 aatactccct gagggagaat ctggcaatgg agggctaaag ctgcccggag cgccagctct
    12901 accacttttt ggttgagaac aggaagcgat cccggtaggc cgaggcaaac cggacaaacc
    12961 agagtgttag gttcttttcc aatgtaatca gtactgcagt ggcaaaacat tttcgcctga
    13021 gtgagtaact gacaatgaac ttctaaacca atggtcgttg taaaatcact cattgttatc
    13081 ctccttaaat agcggaaaag gagaagggag agccagaagt ttttctaaga aaagagccaa
    13141 tcccagtagt ttcccttcct caaaaggtct tcctatgatc tgcaacccca cgggaagccc
    13201 ctcatcatag ccacaattca gagaaagaga gggaagccca gccatagctg agggaacagt
    13261 aaacatgtca tttaagtaca tctcataggg gtctcttcct tttttttcag gaaagaaagg
    13321 aagagaggga gtggtagggg taatcaaaag gtcgcatttc ccgaaagcct cttcgaaatc
    13381 ctgtctcacc agagtcctca ctttcatacc ttttaaatag tattcatcat agtatccgga
    13441 gctcaaagca taagtcccca gaattattct ttttttcacc tctctaccaa aaccaagggt
    13501 gcgagttttg aaatacatat cctgcaggtt ctctccctca acacgcaagc catacaccac
    13561 cccatcatag cgagctagat tagagctcgc ctccgccgct gccactatat agtaagattc
    13621 taaagcataa ctggtatggg gaagagaaac ttctactatt tcaatttcct cttcctttag
    13681 tttctccaga acctcttgaa tttttgtttt gattactttt tctaatccag aaggaaaata
    13741 ctctcgagga actcccactt taagtttacg aatttcttct gaagctggga tttcacttct
    13801 cccgaaagga ggataaggag cacaggtaga atccttatcg tcctttccac tcaccacttc
    13861 taacagagtg atagcatctt ctactttacg agtgattaac ccgacctgat ccagagaaga
    13921 ggcaaaactc accactccat agcgagaaat tcgaccataa gtagggcgca aacccaccac
    13981 tccacagaaa gccgctggtt gtctcaccga gcccccggta tctgaaccta aagcaaaagg
    14041 agcttcaaga gagcaaacgc tggctgcaga accaccggac gatcctcccg ggactctcct
    14101 ccggtcaaaa ggattatagg tgggaccaaa agcactggtt tgagtagaag atcccatagc
    14161 aaactcgtcc atattagttt tacccagtat tatagctccg gcttttttaa gtctctccac
    14221 tactgtagca tcatagggag gagtgaagtt ttccagcatt ttagagccac aagtggtggg
    14281 tatacctcga gtgcagatat tatccttaat agctaccgga agtccccaca gaggcggcaa
    14341 ttcatcaggc aggttttcgg ctttcttttg cgcctcatct tttgcctttt cttcgtttac
    14401 gaacaaaaag gcatgaattt tatcttcccc ttcctttatt ctttctaaaa agagattaac
    14461 caattctaaa ggagagattt ccttgtcctt aaaaagacga tggtaatctc caatggaaaa
    14521 atccctaaac tcttctatct tcattttcct tctcctcttt gtctactacc cggggtacta
    14581 caaaataacc atttgccacc tgtggagctc cttttagcac ctcctcgcca ccttcccctg
    14641 cccggggctc atcagaggag aaaggaggct cactccagga aagatgggaa gtaggagaaa
    14701 tatcctgtag atcaagatcg tttagctttt caaaacgaga aacgacgtta ttaatgtctt
    14761 caaaaaaccc ctggagctct tcttccgaaa gttccagttt agaaacctgc gctatccttt
    14821 tcaattcttg aagggaaatc acttccctca ttttaccacc tccctcttca aattcccagt
    14881 agttgataaa actcttcctc ggtaagaatt tttatccctc tttctcgagc catagctaac
    14941 ttgctccccg ggttttctcc caccaccaca taatccactc gatgagaaac cgtatcagca
    15001 acttttcctc ccctctcctc cacgagagcc cgggctttat ctcgggaaag cttagatagg
    15061 gcaccggtga aaacaaaagt ttttcctgcc agagtccctt cttctcttct ttttaattct
    15121 tccggctcta cccctaagct ctttagctct tctaacatct ggcggttttg agggttagaa
    15181 aaaaattgtc tcactgaatc tgcaaccgta ggaccaatac cttctatagt taaaagttcc
    15241 tcttcctttg cctctttgag tctatctaaa gagccaaaat gctcggctaa aagccgggca
    15301 atgaattgcc ccacatgggg aattcccaga gcatagatga aacgaggtaa ttctcttttt
    15361 ttagacttct ctatggcccg cagcaagtta tcggctgatt tgtttcccat tctctccagc
    15421 tgtaaaagag tttctttatt caaacggtaa aggtcgggta tggttttaac ccatcctcct
    15481 tctactaacc ggtcaatcaa tttctctccc aatccttcaa tatccatggc atcccgggaa
    15541 gcccagtgac gaattctctc cttgatctga gcgggacaac tagcattgat acatcgccag
    15601 gccacttccc cttcctctct caccacccgg gaatgacaga ccggacattc ctcaggcatg
    15661 gcaaaaaccc gctcttctcc cgtccttctt tctttcaaaa cttcagtaat ttcgggaatc
    15721 acctctccag ctcgggtcac catcacccag tcaccaattc taacatcttt ccttttgatt
    15781 tcatcttcat tgtgtaaagt agctctcttt actatcgctc cccctacatt taccggctcc
    15841 agtatcgcca ccggag
//
LOCUS       DAOPSS010000027        14950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_3390_length_14950_cov_7.700235, whole genome shotgun sequence.
ACCESSION   DAOPSS010000027 DAOPSS010000000
VERSION     DAOPSS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 14950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14950
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3390_length_14950_cov_7.700235"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..915
                     /locus_tag="PKN31_02985"
     CDS             142..915
                     /locus_tag="PKN31_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98927.1"
                     /translation="MGWFTFSFKLPDGRHPAELAAQAILKEPKLSLALDTIQKARYIT
                     GVVTKVINDRGFDMELEDEKFTVNSPMLSHILEKRQLVSAHILPNGQGRWLVGPGWLT
                     WPFSPGPHLRPQLKTLFQPNPIEVERFLQEKTKTPEEVKNVEHPQDDTLEAAVARMSE
                     MARKEGKPNLIMSPEEWKNIVLSYMANNDAIGFGEEVHQRAENIDSIEEANKWLALAM
                     NIWNTTPQPDRGGKSAIEIIRQQQGKNRKSRKPFPPDSH"
     gene            1277..1822
                     /locus_tag="PKN31_02990"
     CDS             1277..1822
                     /locus_tag="PKN31_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98928.1"
                     /translation="MKKYIFLGLTIIVFFNFIPTLHTQEISQPQEIPEGYIFYQYQYA
                     FLDISLIYPEDWINYSSQERGVLIDTPDSKGSIMLLGTPLFGEEYTLEKLWAENMENL
                     KSAGATFQESVPDTLSGYSAIKSLYTIKIGEDTHQYICYSSIIGDIFYTFSFHAPVND
                     FEKYREKVLTVVRSVSIKTRE"
     assembly_gap    1961..2060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2061..4600)
                     /locus_tag="PKN31_02995"
     CDS             complement(<2061..4600)
                     /locus_tag="PKN31_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOA98929.1"
                     /translation="MRKAKRGYFLNNLVVILIILLSLSSGNNVFAEVNVGKQLVNPKD
                     FVVEETAVYPAIPGHSLPLAISIVTNFEDFSGKIPLSSTAIELLQKNAFVVIPTPLNI
                     GEQEIYLPPSWEATNPKDDFVAYYKALKNIDVPIFITSDSLLHYYHIFFDTTLMRMER
                     DLFYQDIWTISRELFEASQKDYEQSEGDLKEAARRNMAYLSVALELLKPEKDQVISDE
                     ILKEEYCSPEMDPEYCELMIAGVKDVYGEKASYQYFSETEFERYHFEAPEVVRELVQS
                     EIELIEAHEGWEYSPIFIYQEDYSQYVPRGHYTKSERLKNYFKALMWYGRMTALIEGS
                     PFLSPGESLCSGRIGGIISDYDARIQTLQAFLLAEKFINNSELQECWNRVYALTSFLV
                     GFSDDLGPYEYAEVLQEIGEEEGGAVNIDKLVEKYDQLKEAVRDLPYNPKIYSGLGAC
                     ELLMPCPPLEEDDIQELQKEAERLLSQTKGFRLMGQRFTLDSWLFSEIVSPYSGEYNG
                     PKLPLPTEEKPFTFAWNDDYEEFRNNRPFTWVKTDVAACPPPAEREVRGFPRGLDLMA
                     LLGSQRAWDILENSGDTWYSDYEKVFSQLKDYLDSLRAEDWFGNLYLNWLYVLKSLHT
                     EFGEGYPTFMQTSAWQDKELHTTLASWSELRHDTILYVKQSYTMAERGGAFEPPVVGY
                     VEPVPEFYARLLNLTQMTLAGFKNLIPPEEMENLMIEAGLNRFAEILTRLLDISRREL
                     ENVLLDEEDYDFIENFGSISRDLIGIISGGDLDPEAFQTVLVADVHTEGNTGKVLEEG
                     TGFIKTAVVAYRLPQGHIILGAGPVFSYYEFKQPMDERLTDEAWREIL"
     gene            complement(4625..6337)
                     /gene="amrB"
                     /locus_tag="PKN31_03000"
     CDS             complement(4625..6337)
                     /gene="amrB"
                     /locus_tag="PKN31_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOA98930.1"
                     /translation="MRNLTQLLLLLFLFHVTVVPALGNSGEALSVVSGVVPHHLLAKE
                     IMVDFFSFIARGEQLPDTIILLSPDHLNSAALERGNSFISVNWELNDIRLGDIAVDGE
                     LLKKIATKTRIRPNPGAVLSEFGIMNLLPLIKEHLPGAKIVPILIPANISREEVGQLV
                     NAINQISSAQTIMVASVDFSHYLPSRAASFHDVKSIRVLLNGEEEHFENIEVDSWQSL
                     YAVRLFARLRKKEKPVVIAHKNSVDFLSLSPNETTSYFSVVFEEGEAENEVKAETILF
                     AGDMMLGRGIAELNKKNGIYYPFQKIGRLLRGVDVVFANLEGPVGENPPEFLGDKLQF
                     IFLPPVLEGVSWSRINLVSLANNHITDRGAEGLQETRDWLDRYQICHIGNPLYSYQDE
                     PNPCFSTEHSVFLAFNRVLPFIHREEEIVREIRLAKIDNPGKFIIVSVHWGEEYQLKS
                     SPSQRQLARRMIAAGADIVVGHHPHVVQEIELIQGRPVFYSLGNFIFDQQSIPETQEG
                     LIVGLAVEPDRATCYLFPIQHHLGQPMLMSKGGVDEFLIDLSEKSDKKLGEDIRRGII
                     EIKR"
     gene            complement(6433..7269)
                     /locus_tag="PKN31_03005"
     CDS             complement(6433..7269)
                     /locus_tag="PKN31_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOA98931.1"
                     /translation="MNVRARALTIKFFQYLVMVVVAIIMFFPILWIIASSFKPLIEFN
                     SYPPSLFPSHLYFKNYTELFLQSKIFIYLRNTLILIIGNTLGTLLSSSIVAYPLARME
                     FKGRDLIFGIILATMMVPSFVTIIPQYLLFREFRWLDTFLPMIVPAFFAYPYNVFLFR
                     QFYRTIPRELDEAAKIDGCNSWQIFWRIIVPLSRPIFITIGVLSSIFWWNEFFTPLIF
                     IDSENLKPLSVGSYSFSRTMFIVRWDLLMAMSTIMIVPPMILYLFTRRYIMEGIKTTG
                     IK"
     gene            complement(7286..8170)
                     /locus_tag="PKN31_03010"
     CDS             complement(7286..8170)
                     /locus_tag="PKN31_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOA98932.1"
                     /translation="MAFKKESIVPYLYLLPWVIGFILFTGGPIIAAIFISFTKWDLLS
                     PPVWVGLANFTNMFRAGSEFWGILKVTLIFMVFSVIITMCWSLFTALLLNQKVRGTGV
                     FSFFYFAPAVVPLISLTFVFQLMFSKELGIVNYLLSLTGIEGPNWLMDSRLVLWVVTV
                     LCIYTYFTGQMMLIFDSALKEVPKELYEAAEIDGANAVDKFFKITIPAISPIFFFNLV
                     TGTINSLNTSFALIYPLTGGGPGKATQVMSLDIYVNAFKNFRMGYASAEAVILFAITA
                     ALSLLQFRLAKKWVYYEA"
     gene            complement(8234..9493)
                     /locus_tag="PKN31_03015"
     CDS             complement(8234..9493)
                     /locus_tag="PKN31_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOA98933.1"
                     /translation="MKKRLLVGLLVLGLVCVLAPAAYSADTTVKYAHWNALSEEYNYV
                     KAFEEKYPDIKIEFTLIPEAEYSQKLTTMVMAGTAPDVMCLWECDISRFANGGFVDPL
                     DDYVSESETLTLDDFIPAVQDLIEMQGKLYGLPWCFATEVMYYNKDAFDKAGVPYPDE
                     NWTWEDFENAAKKLTVVENGKVEQWGCDALGFQGLWYSLIGTFGDDIVNDKGQFSIGE
                     GAKEFLNWWYRLTNEEKVVAQPQISTSGIISTDLFVAGKAAMAFNGSWMVSVYRDIEN
                     FKWDIAPIPKGVRQYSTLHTGFFTINSASKVKDAAWKFIEFCMSEEGQELINKAANNP
                     SARISILEKGYYRVQGPMGPENWDAIDKTAQFAQWGYVLLPSGLTFDIVKDFNAAILG
                     QMGIDEVVGKAIDRAKEILGEEGVASE"
     gene            complement(9967..11820)
                     /locus_tag="PKN31_03020"
     CDS             complement(9967..11820)
                     /locus_tag="PKN31_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOA98934.1"
                     /translation="MSNTFVVDTSKSKYVRLKPVSISSVKLTDSFWANRIKLMGEVTL
                     PLQYQIMEETGRMDNFRIAAGKIPGEFSGFFFNDTDVYKWIEAVSYFLAINKDEKLDK
                     LVDSVIEEIRDAQDTDGYLDTYFTFSRKQDRWTNLKDMHELYCAGHLFQAAIAHYRVT
                     GKTNFLDIAIRFADHITEVFGPGKREGVPGHPQIEMALVELYRETNDRKYLDLAKFFV
                     DERGKGIIGGGTYHIDHKPFRELDEIVGHAVRSLYLNSGATDLYMEIGDKTLWSALER
                     LWDNLVTKKMYITGTAGARHEGESFGDPYELPNETAYAETCAAIANFMWNYRMLLTSG
                     EGRFADVMELALYNGVLSGISIDGKEYFYVNPLADRGGHRRQRWFACACCPPNVARTI
                     TSIPGYMYSISPEGIWVNLYASNVSEFDINGSKVAIEEKTDYPWEGLVEFKVDADSSF
                     SIFLRKPEWSREFSLNVNGERFEPALEKGYMVINRRWQKGDTVTLDLHMRPEFLESHP
                     GVKDNVGRVAIRRGPVVYCVEAEDNPGVDVWDLEIPKDTKISKEYRDTLGGIVAIKGE
                     GYINEPGLWEKLYLPLDEANRLKEGIEFTAIPYYAWANRTPGPMEVWIKTK"
     gene            complement(11878..12558)
                     /locus_tag="PKN31_03025"
     CDS             complement(11878..12558)
                     /locus_tag="PKN31_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOA98935.1"
                     /translation="MKPINRVKLTESIMEAIVEYASSNDLKVGDRLPSERELSSALKV
                     SRPLLREALRKMESLNLIEVLPGKGTFIKVPLKDNMSYLVLHIDEEKDKVLEVLKIRR
                     ALEKLALEEAIKNITDDEIKELERRVNLLEEKQRRGDDSREENWAFHSLIYRLAGNKL
                     LFDILDGLKEFHLFWEDPLECPSFAEKTYFFHRELFKCIEERDLKRAHAVTDKLLDML
                     EGEIKKSY"
     gene            complement(12749..13498)
                     /locus_tag="PKN31_03030"
     CDS             complement(12749..13498)
                     /locus_tag="PKN31_03030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOA98936.1"
                     /translation="MGKLSFHTALVTGGSRGIGRAIALSLARAGARVAVNYLQNEEAA
                     QEMVEEIESSGSMAIKVKGDVSQKKEVEKMIEQVQQKLGIIDILVNNAGIGDPYYSSA
                     LSIEEETWDKILAVNLRGTFLVSQAVLPDMINKKWGRIINISSTSGITGGTSGAHYAA
                     AKGGVIALSKALSQEFAPYGITVNVVAPSKIDTDLLRSVTPPGKEKEVIKKIPVGRLG
                     TPQDVAEAVLFFSREEASFITGQVLVVSGGY"
     gene            13593..>14950
                     /locus_tag="PKN31_03035"
     CDS             13593..>14950
                     /locus_tag="PKN31_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOA98937.1"
                     /translation="MRKQEPLFLGIDLGTQGVRCLVSTPQGEIVAEGKEKISHIYREG
                     ERFEQEPEEWWEVTLRALQGTLRDLRKKGKDPNSIQALSCDSTSGTVLALNREGRTLS
                     RAIMYNDGRAKEEAEIINREATSFTERSGYQFGASFALAKVLWWKRNNPELFDRAHFF
                     LHAADFIVGKLTGEWGVSDTSNSLKMGYDLIDFKWPSFIESRLGIPVYKLPRVVNPGE
                     VVGNVRPEIAQKLGLSPHCKVVAGCTDGTASFLASGATRPGDVSCALGTTLVVRSISY
                     QLIKDPEGRIYCHRHPEGYWLPGGASNTGGECLEVYFPGEDYREWDEKITLENLPTSL
                     LVYPLTRRGERLPFSSGEASFFQVGEEKSREEFYAACLEGVGYIERLSLELLESLGAD
                     TITRVFASGGGSRSPIWRKIRANILNRPVFLAQTTESAFGSCVLSASPFWGKISQAVQ
                     EMV"
BASE COUNT         4380 a         3422 c         2739 g         4309 t
ORIGIN      
        1 gaggccatag ggataagcct gcagggggag tgatttatat gactaatagg ttggtggaat
       61 ggtgttatac gaatgagtcc attgctaacg agtgattatc tggctgatac tggtagcttt
      121 gccggccgat agaggcggtt tatgggttgg tttaccttta gttttaaatt acctgatggg
      181 cgacatccgg ctgaattggc agctcaggct atactaaaag agcccaagct ttctctcgct
      241 cttgatacca tccaaaaagc aagatatata actggcgtgg tcaccaaagt tattaatgat
      301 cgaggttttg atatggaact ggaagatgag aagtttacag tgaacagccc gatgttaagt
      361 catattttgg aaaaaagaca gcttgtctct gcccatattt tgcccaacgg gcaaggccga
      421 tggttggtgg gtccaggctg gttaacttgg ccattttctc cagggccaca cttgcgtcca
      481 cagttaaaaa ctctctttca gcctaatcct atagaagtag agagattcct tcaggaaaaa
      541 acaaaaacac cggaagaggt aaaaaatgtc gaacatcctc aagatgatac gcttgaagca
      601 gcggtagccc gtatgtctga gatggccagg aaagaaggaa aaccgaatct catcatgtcg
      661 cccgaggaat ggaaaaatat cgtcctatcc tatatggcaa ataacgatgc tatcggcttt
      721 ggtgaagaag ttcaccagcg agcggagaat attgactcta ttgaggaggc aaataagtgg
      781 cttgccctgg ctatgaacat ctggaataca acacctcagc cagaccgggg aggaaaatca
      841 gctatcgaaa taatacgcca gcaacagggt aaaaatagaa aatctcgaaa gcctttccca
      901 ccagatagcc attagctggc catttggttt ttaagcgagc tacccggtgg aaaaaaggag
      961 taacagcaac tacccctgta actgaacctg cattacctaa atcgctgcat tttataattt
     1021 atgtttttca tgtagatttt tgatctcgta ttactgcaaa ataaaaaggt ggtttagcaa
     1081 aagttcagca gactataaaa ggtcgctcta taaattcagc cagattatat tgtaaataaa
     1141 caagaataga tttttagaaa aacctctcat acccgataga aaacattttg aaaagagact
     1201 tttcgacatt ttataggaaa taatgctgat aaaaaactat tctcttaaga gaataacaaa
     1261 taaaaggagg gaaaatatga aaaaatatat tttccttggt ttaactatca tagtattttt
     1321 taattttatc ccgacattgc atactcagga aatttcacaa ccccaggaaa ttccggaagg
     1381 atacattttt taccaatatc aatatgcttt tctggatatt tccctgattt atcccgaaga
     1441 ctggatcaac tattcttccc aggagcgggg agtgttaatt gacaccccgg actccaaagg
     1501 ttcaattatg cttctcggca cccctctttt cggggaagaa tatactttag aaaaactatg
     1561 ggcggaaaat atggaaaatc tgaaatcagc cggtgcaacg ttccaggaat ctgttcctga
     1621 taccctctct ggatattcgg ccatcaaatc tttgtatact attaaaattg gcgaagacac
     1681 ccaccaatat atctgctaca gctccattat cggagacata ttttacactt ttagctttca
     1741 tgctccggtc aatgattttg aaaaataccg ggaaaaggtg cttaccgttg tccgttctgt
     1801 atctatcaaa accagagaat aaaacgttaa accgccatcc ccgatagaat aataaattat
     1861 tctgaaaaac ttttcaccca gtccggttgt ggtggaggat tgctatctaa tatttctctc
     1921 caggcttcat cggtcagccg ctcatccata ggttgcttaa nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn tctaatattt ctctccaggc ttcatcggtc agccgctcat
     2101 ccataggttg cttaaattca taataactga aaaccggtcc ggctcccaaa atgatatgtc
     2161 cctgaggtaa cctataagcc acaaccgctg tttttataaa accagtacct tcttctaaaa
     2221 ctttcccggt atttccctcg gtatggacat cagcaactaa aacagtctgg aaagcttctg
     2281 gatctaagtc tcctcccgat atgataccga ttaaatccct ggagatgctg ccaaagtttt
     2341 cgataaaatc gtaatcttct tcatccagga gcacattttc taattctctt ctggaaatat
     2401 ccagcaatct ggtgagtatc tcggcaaatc tgttcagccc tgcttcaatc atcaggtttt
     2461 ccatttcttc cggtggaatt aaatttttaa atcccgcgag cgtcatctga gttaagttta
     2521 aaaggcgagc atagaattca ggcactggtt caacatagcc taccacagga ggctcaaacg
     2581 ctcctcccct ttcagccatg gtatagctct gtttcacata caatatagtg tcatgtctta
     2641 gctcggacca ggaagcaagg gtggtatgga gctctttatc ctgccaggcg gaagtctgca
     2701 taaaggtggg gtaaccctcc ccaaactcgg tatgcaacga ttttaataca taaagccagt
     2761 ttagataaag attaccaaac caatcttccg cccggagaga atcaagataa tctttcagtt
     2821 gggaaaatac cttttcatag tcgctatacc aggtatcacc agaattttct aaaatatccc
     2881 aggctctctg tgaacccagc aaagccatta aatccaatcc tcgaggaaac ccccgaactt
     2941 ctctttctgc cggaggggga caggctgcaa catcagtctt cacccaggta aagggccggt
     3001 tattcctgaa ctcttcataa tcatcattcc aggcgaaagt aaaaggtttt tcctcggtag
     3061 gaaggggaag ttttggacca ttatactctc cactgtaagg agaaacaatt tcagagaata
     3121 gccaggaatc caaggtaaac ctctgaccca tcaggcgaaa tcccttggtc tgagatagaa
     3181 gtctttcggc ctctttttgc aactcctgaa tatcatcttc ctcgagagga ggacagggca
     3241 tcagcaactc acaagctccc aatccactgt atattttggg gttataaggg aggtctctca
     3301 ccgcttcttt taactggtca tatttttcaa ccaatttatc tatgttaacc gctcctcctt
     3361 cttcctctcc tatctcttga agcacttcgg cgtattcgta aggtcctaaa tcatcggaga
     3421 aacccaccag aaaagaagta agggcgtaaa ctctgttcca gcactcctga agctctgagt
     3481 tgttgataaa tttttcggct aacaaaaacg cttgcagagt ctgaatccgg gcatcatagt
     3541 cagaaataat tcctcctatc cgaccactgc agagagattc tcctggtgat aaaaaaggag
     3601 agccttctat taaggccgtc attctaccat accacatgag cgctttaaaa tagtttttca
     3661 acctttccga tttagtataa tgtccccggg gaacatattg agaataatct tcttgataga
     3721 taaaaatggg tgaatattcc caaccctcgt gtgcttctat caactctatc tcagattgca
     3781 cgagctccct aaccacttca ggggcttcaa aatggtagcg ctcaaactct gtctcactga
     3841 aatattgata gctggctttc tctccgtaaa catctttcac accagctatc attagctcac
     3901 aatattccgg gtccatttct ggagaacagt attcttcttt taagatttca tcgctgatta
     3961 cctggtcttt ctcgggcttt aaaagctcca gggcgacaga aagataagcc atatttcttc
     4021 gggctgcttc ttttaaatca ccctccgact gctcataatc tttttgtgat gcttcaaaca
     4081 attctcggct gatagtccag atatcctgat agaaaaggtc tctttccatc cgcatcagcg
     4141 tggtatcaaa aaagatatgg taataatgca aaagagaatc agaagtaatg aaaattggta
     4201 catcaatatt ttttaacgct ttgtaataag cgacaaaatc atctttggga tttgttgcct
     4261 cccaggaagg aggaagataa atttcctgtt ctcctatatt aaggggggtg gggatgacca
     4321 caaaggcatt tttctgcaac aactctattg cagtagaact taagggaatc ttccccgaaa
     4381 aatcttcaaa attagtaaca atagagatag ctaaaggcaa ggaatggccg ggaatagcag
     4441 gataaactgc ggtttcctct accacaaaat ctttcggatt aaccagttgc ttaccaacat
     4501 taacttcagc aaagacattg ttccccgagg aaagagatag caagataatc aggataacta
     4561 ccaaattatt taaaaaatag cctctttttg cttttctcat ttttacccct ccttattttt
     4621 ccatctatct ttttatctct attatacctc tccttatatc ttcccctaac tttttatcag
     4681 acttctcaga taggtcgata agaaattcat ccaccccgcc tttactcatc agcatcggct
     4741 gacctaaatg gtgttgtata gggaaaagat aacaggtggc cctatcaggc tcaacggcca
     4801 agcccactat cagtccttcc tgagtttcag gtatactttg ctggtcaaag ataaagttac
     4861 ccagagaata aaagactggc ctaccttgta tcaattcaat ttcctgtacc acatggggat
     4921 gatgtcccac gacgatatcc gctcccgcag caatcattcg acgagccaat tgccgctggg
     4981 atggcgagct ttttaactga tattcttctc cccagtgcac acttacgatt ataaatttac
     5041 ccgggttgtc tattttggct aatcttattt ctctaacaat ttcctcctct ctgtggataa
     5101 acggtagcac tctattaaaa gccaaaaaaa cggagtgttc agtagaaaaa cagggatttg
     5161 gctcatcctg atagctatat aaaggattac caatatggca aatctgatat ctatccagcc
     5221 aatctctggt ctcttgtaag ccttctgctc ccctatctgt aatatgatta ttggctaagg
     5281 atacgagatt aatcctactc cagctaaccc cttcaagaac cgggggaaga aagataaatt
     5341 gtagtttatc acccaaaaat tccggcgggt tttccccaac gggaccttct aagttggcaa
     5401 aaacaacatc aacgcctctc agtaatcgtc caattttttg aaaaggataa taaataccat
     5461 tctttttatt cagctctgct atccctcgcc ccagcatcat atccccagcg aataaaattg
     5521 tttctgcctt gacctcattt tcagcttctc cctcctcaaa aaccacgctg aaataggaag
     5581 tagtttcatt tggagaaagg gataagaaat ccacagaatt tttgtgagca atcacgaccg
     5641 gtttttcttt tttccgcaac cgggcaaata acctcacagc atacagtgat tgccagctat
     5701 ccacctctat attttcaaaa tgctcttcct caccgttcaa taaaacccta atgcttttaa
     5761 cgtcatggaa tgatgctgcg cgagagggga ggtaatggga aaaatcaaca ctggctacca
     5821 taatagtctg agcggaggaa atctgattaa ttgcatttac cagttgaccc acttcttccc
     5881 gggaaatatt cgctggtatc agaataggca caatttttgc tcctggaaga tgctctttga
     5941 ttaaaggcaa gaggttcata atgccaaatt ctgaaagcac cgcgcccggg tttggcctta
     6001 tcctggtttt agtagctatc ttttttaaca attccccgtc aacagctata tcacccagcc
     6061 tgatatcatt caattcccag ttgacactga taaaggaatt tcctctctcg agagccgcgc
     6121 tattcaaatg gtccgggctt aatagaatga tggtgtcagg aagctgctct cctctagcaa
     6181 taaaactgaa aaaatccacc ataatttctt tagccagcag gtgatggggc acaacaccgg
     6241 aaaccacaga caatgcctca ccagagttac cgagagcagg caccacggtt acatggaaaa
     6301 gaaacaaaag caataacagt tgagttaaat ttctcatatt gatgatattt tacagcattt
     6361 tacagcatgc gagttcctgc acagaagata taaaacatcc gcttcttgca ggttttcagt
     6421 atacaaatgt atctatttta tccccgtagt cttaattccc tccattatat atctccgagt
     6481 aaagagatat aaaatcatgg ggggtactat cattattgta gacatagcca tcagaagatc
     6541 ccatcttacg ataaacatag tccgtgaaaa actataagag cccacgctta aaggcttcag
     6601 gttttctgag tctatgaata ttaaaggcgt gaaaaattca ttccaccaga atatggatga
     6661 caaaacccca atggttatga atataggacg ggaaagaggc actattattc tccagaatat
     6721 ttgccagcta ttacagccgt ctattttcgc cgcttcgtct aactccctgg gaattgtcct
     6781 ataaaattgt ctgaaaagaa aaacattata gggataggca aaaaaagcag gtactatcat
     6841 tggtaaaaat gtatccagcc atctgaactc tctaaaaaga agatattgag gaataatggt
     6901 aacaaagctg ggcaccatca ttgtcgccag tataatccca aatataaggt ctcgtccttt
     6961 aaactccatc ctcgccagag gataagccac aatagagctc gatagcaaag tccccagggt
     7021 atttcctatg attaaaatta gcgtgtttct tagataaatg aatatcttac tctggagaaa
     7081 caattcagta tagttcttaa aatagagatg tgatggaaac aaagatggag gataagagtt
     7141 aaattcaata agaggtttga aagaagaagc gataatccat aaaatgggga agaacattat
     7201 tatggcaaca acgaccatca ctaaatactg aaaaaatttt attgttagtg ctcttgctct
     7261 tacgttcata gtcttctcat cctcctcaag cctcataata tacccatttt ttggccagtc
     7321 ggaactgcag tagagatagc gctgcagtaa ttgcaaataa aatcaccgct tctgcactcg
     7381 catatcccat cctgaaattt ttaaatgcgt taacatagat gtctaaactc atcacctggg
     7441 tagcttttcc aggcccacca cctgtaagag gataaatgag agcaaaggaa gtattcaaag
     7501 agttaatagt acctgtgact aaattaaaga agaaaatagg agatatagca ggaatggtga
     7561 ttttgaagaa cttatccaca gcattagcac catcaatctc tgccgcttca tagagctctt
     7621 tggggacttc tttcagagcg ctatcaaaaa tcaacatcat ttgccccgta aaataagtat
     7681 aaatacaaag gaccgttaca acccataaca ctaacctcga atccatcaac cagtttggac
     7741 cttctatccc agtcaaagat aagagataat ttactattcc cagctctttg ctgaacatca
     7801 gctggaatac aaatgttaaa gatataaggg gaaccaccgc tggagcaaaa taaaaaaagc
     7861 taaaaacacc agtaccccgg accttttgat ttaaaagtaa agcagtaaaa aggctccagc
     7921 acatagtgat aatcacagag aacaccatga aaataagagt aacttttagt atgccccaga
     7981 attctgaacc agcccggaac atattggtga agtttgcaag tcccacccag acagggggag
     8041 acaacaaatc ccacttagta aaactaataa agatagcagc aattatgggg cctcccgtaa
     8101 ataagataaa acctattacc cagggaagta aatacagata tggaactata gattcctttt
     8161 taaaagccat gacgaatccc ttcctcctta aattaaaggg ggattctcgc agaatccccc
     8221 tttaatttaa acatcattca gaagcaactc cttcttctcc cagaatctct tttgccctgt
     8281 ctattgcctt tcctactacc tcatctatac ccatttgtcc taatattgcc gcattaaagt
     8341 cttttacaat atcaaaggtc agcccggagg gaagaagcac atatccccac tgagcaaact
     8401 gggcagtctt atcaatggca tcccagttct ctggccccat aggtccctga actctgtagt
     8461 aacccttttc aaggatagag atgcgagctg acggattatt agcagcttta tttattaact
     8521 cctgtccctc ttcactcatg cagaattcga tgaatttcca tgctgcatct ttcaccttgc
     8581 ttgcagaatt aatggtgaag aacccggtgt gaagtgtaga atactgtctt acccctttag
     8641 gaattggggc aatgtcccat ttaaagttct ctatgtctct atagactgag accatccagc
     8701 ttccattaaa tgccattgct gctttaccag ctacaaagag gtctgtagaa attatacctg
     8761 aagtacttat ttgtggctga gcgaccactt tctcctcgtt ggtcagacga taccaccagt
     8821 taagaaattc tttagctcct tcacctatag aaaattgtcc tttatcattt acaatgtcgt
     8881 ctccaaaagt cccgatgaga gaataccaca gaccctggaa gccaagagca tcacatcccc
     8941 attgttctac tttgccattc tccaccacag tcagcttttt ggcagcgttt tcgaagtctt
     9001 cccaggtcca gttttcgtcc ggatatggaa cacctgcttt atcaaacgca tctttgttat
     9061 agtacattac ttctgttgcg aaacaccagg gaagcccgta gagtttcccc tgcatttcta
     9121 tcaggtcttg aactgcagga ataaaatcat ccagagttaa agtctcgctc tcacttacgt
     9181 aatcatctaa tggatccacg aagccgccgt tagcaaatct agatatatca cactcccaca
     9241 ggcacatgac atctggagca gtgccagcca ttaccatagt agtaagcttt tgagaatatt
     9301 ctgcttcagg aattagcgta aattctattt taatatcagg atatttttct tcaaatgcct
     9361 taacatagtt gtattcttca gaaagagcgt tccaatgagc atattttact gtagtatcag
     9421 ctgaataagc agcaggcgcc aatacacaca ccaatcccag aacaagcaaa ccaacaagta
     9481 accgcttctt catgttactt tccctccctc tctttagagt tttgtcaggt cctaataatt
     9541 ttccttttaa ataaatatct taaatttcat caccttacac aaaattacac aaaattacta
     9601 ccctcttctt ccgaagcaac ctctttagac ttaatatcgt tgtcgcattt ttcccttctc
     9661 aaatcacccc cttgtgaatg ccagcacgat aaactggcaa accacctatt tctttactgg
     9721 tcttaccatc ttaccaaata aaagtataat catcctgtaa aatattgtca atccttcact
     9781 gttattttta tgttactgta taaccttact ttgaagattc caaaaccagg tcggaaaccc
     9841 gatcccgagt gaatataaat attactcagc ggtctatcga ataaattcct aaaaaaaatg
     9901 cggttacaca caaaacttat acatgtaacc gcagccagtt tcagttcttg agaatcggaa
     9961 aaattcttac tttgttttta tccagacctc cataggcccc ggagtcctgt tagcccaggc
    10021 gtaataggga atggcagtaa actctattcc ttctttaaga cgattagctt cgtcgagagg
    10081 cagatagagt ttctcccaga gtccgggctc gttaatgtat ccttctccct ttatggccac
    10141 tattccaccc aatgtatctc tatattcctt actaatcttc gtatctttcg gtatctctaa
    10201 gtcccagaca tccaccccgg gattatcctc agcctccaca caataaacaa ctggccctct
    10261 tcgaatagct actctcccta cattatcttt cacgcctgga tgactctcca gaaactctgg
    10321 cctcatatgt aaatctaagg ttactgtgtc tcccttttgc catcttctat ttattaccat
    10381 atatcccttt tctaaagcgg gttcaaatct ttctccatta acgttcaaag aaaactccct
    10441 gctccattcc ggttttctta aaaagatgga aaatgaagaa tctgcatcta ccttaaattc
    10501 tactaatccc tcccaggggt aatctgtctt ttcttctatt gccactttag agccattaat
    10561 atcaaattca gacacattac tggcatagag attgacccat atcccctcag gagatattga
    10621 atacatataa ccaggaatag aagttatagt cctcgctaca ttaggcggac aacaggcaca
    10681 ggcaaaccat ctctgtcttc tgtgaccgcc tctgtctgcc agaggattta cataaaagta
    10741 ttccttccca tctatggaaa ttcccgacaa aactccatta taaagtgcca attccatcac
    10801 atcagcgaat ctaccctcgc ctgatgtcag aagcattcta tagttccaca taaagtttgc
    10861 gatggccgcg caggtttctg cataagccgt ttcattgggt agctcatacg gatctccaaa
    10921 agattctcct tcgtgtctcg cccccgcagt cccggttata tacattttct tggttaccaa
    10981 gttatcccat aatctctcca aagcactcca taaagttttg tcccctatct ccatatataa
    11041 atctgtagcg ccgctattca ggtatagaga cctcactgca tgaccgacaa tctcatccag
    11101 ctctctaaat ggtttatggt ctatgtgata agtacccccg ccaatgatgc cttttcctct
    11161 ctcatctaca aagaatttag ccagatccag atatttccgg tcattggttt ccctgtaaag
    11221 ttctacaaga gccatctcta tttgcggatg tccgggaact ccctctctct tccccgggcc
    11281 aaatacttcg gttatgtggt ctgcaaatct tatcgctata tctaaaaagt ttgtcttccc
    11341 tgtaaccctg tagtgagcaa tggccgcctg gaagagatgc ccggcacagt acaattcatg
    11401 catatctttc aagttagtcc agcggtcttg ctttctggaa aaagtaaaat aagtatctaa
    11461 ataaccatct gtatcttgag catctcttat ctcttctata acgctatcta cgagtttatc
    11521 cagtttctca tctttgttaa tagccagaaa atatgatacc gcctctatcc atttgtagac
    11581 atcagtatca ttaaagaaaa aaccagaaaa ttctccagga atctttcccg cagcaatcct
    11641 gaaattatcc atcctcccgg tttcttccat aatctgatat tgaagaggta aagtgacctc
    11701 gcccataagc tttattctat ttgcccagaa gctatctgta agcttcactg aacttataga
    11761 gacaggcttc aacctgacat atttactttt gctcgtatca actacaaagg tattggacat
    11821 aaacattcct ccttagcatt aaattgtatt ctttcccaat attacttcag aaagatgcta
    11881 ataactcttt tttatttcac cttcgagcat atctaacaat ttatccgtca cagcatgcgc
    11941 tcttttgagg tctctctctt ctatacattt aaaaagttct ctatggaaaa aataagtctt
    12001 ttctgcaaaa gaaggacatt ccagaggatc ttcccagaaa aggtgaaatt cctttaaccc
    12061 gtccaaaatg tcaaacaaga gtttattacc cgccaaacga taaatcaggc tatgaaaagc
    12121 ccaattctcc tccctgctgt catctcccct tctctgcttt tcttcaagga gatttactct
    12181 tctttccagc tcttttattt catcatcggt aatattctta atggcttcct cgagagccag
    12241 cttttctaag gctcttctga tttttagaac ctctaaaact ttatcttttt cctcatctat
    12301 atgaagaact aaataagaca tattgtcctt caggggaact ttaataaatg tcccctttcc
    12361 cggcaacacc tcaatcaggt tcagactctc catcttcctt aacgcttctc tcaacagagg
    12421 gcggcttacc ttaagagcac tggatagctc cctttccgat ggaagtctgt cccctacttt
    12481 tagatcgtta gaagaggcat attctactat agcttccatt atgctttcag ttaattttac
    12541 tctattaatt ggtttcatag ctcacctggt cttaccatct taccgaggag tataaccaca
    12601 ctacagggtg ttgtcaaccc ccaagaggct ttagacacac ctgttgagaa agcagagcgc
    12661 tccccccatc ataagagaaa gaaaagaatg ggattttatc cctatttttc tagtagcact
    12721 tcttttcaaa cttaccggcg aaagtggttc agtatcctcc cgataccacc agaacttgcc
    12781 cggtgataaa acttgcttct tcccgggaga agaaaagcac tgcttcagct acatcctgag
    12841 gggtgcctaa gcggccaacg ggtattttct ttattacttc cttttctttg ccgggaggtg
    12901 ttactgaacg caaaagatca gtatcgattt tactgggagc gacaacattt acagttattc
    12961 catagggagc aaattcttga gataatgctt tacttaaagc tataactcct cctttagcag
    13021 ccgcataatg agctcctgaa gtgcctccgg ttattcccga agtagaagat atattgataa
    13081 ttctccccca ttttttattt atcatatcag gtaaaacagc ctgggaaact aagaaagtgc
    13141 cccgtaaatt tactgccaga attttatccc aggtttcttc ttctatcgaa agagccgaag
    13201 aatagtaagg gtcacctatc ccggcattat ttaccagtat atcaataatt cccaattttt
    13261 gttgaacttg ctctatcatc ttctcaactt ctttcttttg agatacatct ccctttactt
    13321 ttatagccat agaccctgaa ctctctatct cttctaccat ctcttgagcg gcttcctcat
    13381 tctgaagata attcaccgct acccgggcac cagcccgagc caaagataaa gcaatggccc
    13441 gaccgatacc ccggcttcct cctgtaacca gggcggtatg aaaagaaagt tttcccatag
    13501 ttttttctcc ttttgcatac ttgaatgatt agatttctct ttaaataaat tataatagaa
    13561 tcatcaaggc aaaagaaagg aaaattggta aaatgagaaa acaagaacca ttgtttttgg
    13621 gaattgattt aggaactcaa ggggttagat gcctggtttc tactcctcag ggagagatag
    13681 tggcagaagg caaagaaaaa atttctcata tttaccggga aggagaacgt tttgagcaag
    13741 aaccggagga atggtgggaa gtaactcttc gagctttgca gggaactctc cgcgacctca
    13801 ggaaaaaagg aaaagatccg aattccattc aggctttatc ctgcgattct acttctggca
    13861 ccgtcttggc tctcaaccga gaaggacgaa ctctcagtcg ggctattatg tataatgatg
    13921 gaagagctaa agaggaagca gaaattataa accgtgaagc tacctcattt accgagcggt
    13981 caggttacca gtttggagct tcttttgcct tagctaaagt attgtggtgg aagagaaata
    14041 acccggaatt gtttgataga gctcattttt tcctccatgc tgctgatttt atagtgggaa
    14101 aattgactgg agagtggggg gtgtcagata cctctaactc cttgaagatg ggatatgacc
    14161 ttatcgattt taaatggcct tcttttatcg aaagccggct gggtattcct gtctacaaac
    14221 ttcctcgggt ggtcaatccc ggggaagtag taggtaatgt cagacccgag atagctcaaa
    14281 aactgggttt gtccccccac tgtaaggtgg tggcaggatg caccgatggc acagcatctt
    14341 ttttagcttc cggtgctacc cgtccggggg acgtatcctg tgctttagga actactcttg
    14401 tggtccggag tatcagttat caattaatca aagacccgga ggggcggatt tactgccatc
    14461 gccaccctga aggttattgg ttaccaggag gagcgagtaa caccggagga gaatgtctgg
    14521 aggtatattt tccaggagaa gattatcgtg aatgggatga aaaaataact ctggagaacc
    14581 ttcccacctc tcttctggtt tatcctctca cccggagagg agagcgtctg cctttttcct
    14641 caggggaagc ttcttttttc caggtgggtg aagaaaaaag cagggaagaa ttttatgctg
    14701 cctgtttaga gggagtggga tatatagagc gactgagcct ggagctttta gaaagtttag
    14761 gagcagatac cataactcga gtgtttgctt caggaggcgg ctctcgtagc cctatctggc
    14821 gaaaaatccg ggccaacatt ttaaatcgtc ctgttttcct ggctcaaact accgaatcag
    14881 cttttggctc ttgcgttcta tcagcttccc ctttctgggg gaaaatatca caggcggtgc
    14941 aagaaatggt
//
LOCUS       DAOPSS010000028        14860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_3424_length_14860_cov_7.967781, whole genome shotgun sequence.
ACCESSION   DAOPSS010000028 DAOPSS010000000
VERSION     DAOPSS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 14860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14860
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3424_length_14860_cov_7.967781"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            451..630
                     /gene="rpsU"
                     /locus_tag="PKN31_03040"
     CDS             451..630
                     /gene="rpsU"
                     /locus_tag="PKN31_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOA98938.1"
                     /translation="MTEIKVGKGESIDEALRRFKKKCQRNGVISEMKRREHYEKPSEK
                     KRKKAQAAARRKRRG"
     gene            627..1082
                     /locus_tag="PKN31_03045"
     CDS             627..1082
                     /locus_tag="PKN31_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOA98939.1"
                     /translation="MKEVSSLKVQIEEQMKAALKAHDQLRLDTLRLLLSEIKNKEIEK
                     RGNLEEGEIVALIRKEIKKRQESEEYFEKGNRSELLEKAEKEIAILQDFLPPQLSEEE
                     VERVINDVLASWPGEASLFPVMKEVMSRLQGRAGGEVISKVVCQKINVG"
     gene            1092..2084
                     /locus_tag="PKN31_03050"
     CDS             1092..2084
                     /locus_tag="PKN31_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOA98940.1"
                     /translation="MSQGIDLKDREVRFSLESVENIKALFGYLDSKIRVVESVFKVEV
                     TIEEEDLVIRTKSESGEVSLARDFLQELSSFSPPEELSSDDLQKMALSFKENKKAWWN
                     DKVEPTILTTAYHKPVRAKSPGQMKYVQTVRNADITFCIGPAGTGKTYLAMAMACAAL
                     QTKKVSRIVLVRPAVEAGESLGYLPGDLKEKIEPYLRPLYDALYEMLSPDKFQKYSEK
                     GVIEVAPLAYMRGRTLNDSFIVLDEAQNTTPEQMKMFLTRMGFGSKVVVTGDITQIDL
                     PSGKKSGLVVVQEILKDIKGIEFVYLNEKDVVRHQLVQKIILAYEKFEEKALSR"
     gene            2059..4152
                     /locus_tag="PKN31_03055"
     CDS             2059..4152
                     /locus_tag="PKN31_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOA98941.1"
                     /translation="MRKKLSLAKSHLLSFRFLNFLLYGIMLFFLFWFSSWGEEWKIKE
                     GEVVSRDIIAPRSVEIIDIPSTAEAQKRLLSAIPPVYRVDPQTLDKIEQELEEFFKSL
                     EEARPTYSLLSTDFKDTFSKKWRITPGVLEWLIASPQEEYERVKGVTFDIINRYMANP
                     IREEELEEKILEAYSELERMDLGESEIRVAEALVFRFLRPTAVVDSEGTQRERLKALE
                     SLQPVKRIIKRGEVILRKGEIATKEDIEILRAMGFGGEGDQWFQLLAWALVITGAVVA
                     EYFYIRKFVPSLLEDDSLLLLRLIAVGGIVAMNTLTFRLSSFFIIISALPLILLPLLG
                     EDYALGESLILFPLILLGDRVDFIQGFYIYVNLFLPLFWLSKISKTGDLVKAGMMMAF
                     TNMGLSFVFGLQEGKELTKVLADAFYGWGGGMGGAVIALGGITLLESIFHITSDFRLM
                     ELLNPTHPLLKRLLMEAPGTYSHSLMVANLAEAAAERIGANPFLVRVGAYYHDVGKIK
                     RPYFFIENQMEGHNAHNRLSPNLSSLIIQSHVKDGVEIARQYNLPPEIQEIIACHHGT
                     TIIRYFYDKAVKERKDEVREEEFRYPGPLPRTVEQALVFLADSVEAAVRSATRPTPSR
                     VDAIVNGILQKYLQEGQLDESPLTMRDIRQIAQAFVVVLNGMIHSRIPYYPEEEALKI
                     VRGKDIKDEPGRNSK"
     gene            4127..4957
                     /locus_tag="PKN31_03060"
     CDS             4127..4957
                     /locus_tag="PKN31_03060"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOA98942.1"
                     /translation="MSRVEIVNRTRESFLTRKKVQKIVDFVLKEEGKDFPGCLSIAAV
                     DEEEIRSLKKRFFHQDVCTDVISFFYGFSDEDDIWGEIIICPAVVKQEAHVRGESEER
                     ALSIVLIHGLLHLLGYEDEDAEKARVMEERQEALYRLLEEEEEREKLIKRAIKVREYA
                     YAPYSSFPVGAALLGYDGDIFTGCNVENASLGLTICAERVAIFKAISQGVREFKRIAI
                     IADPSSYCFPCGACRQVLYEFAPQLEVIAAKSDRDYEIVSLHSLLPRAFFLRNDDRKE
                     "
     gene            4963..5847
                     /gene="era"
                     /locus_tag="PKN31_03065"
     CDS             4963..5847
                     /gene="era"
                     /locus_tag="PKN31_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOA98943.1"
                     /translation="MAFRSGFVTIWGQPNVGKSTFLNYVLGRKVAIVSSKPQTTRQVL
                     KGILNLPDAQVVFLDTPGLHSPRDEFGKWMVQEAEKALDGVDVLLYMIAPDNREEEDR
                     KYLNSLHSFTGPVFLVLNKKDLVQEEEARKILSRFSNLFPFQGKAAVSSITGEGISEL
                     LSEIVEALPPGPPYYGEDVISDIWEKDIVAEIIREKIWWYTYQEVPYGVEVRVEEFKD
                     RGDILYIRAIIYVERDSHKKIVIGQEGKMLKKIGEEARKEIEFNWGKKVYLDLWVKVE
                     KNWRKRPEVLGRWGYRTK"
     gene            5844..6620
                     /gene="recO"
                     /locus_tag="PKN31_03070"
     CDS             5844..6620
                     /gene="recO"
                     /locus_tag="PKN31_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOA98944.1"
                     /translation="MRERFNRYWKDEGIVIRSTNLGERDRLVTLFTQKRGKIRALAKG
                     ARKVGNRFGASLDVFNYSEFMFYSGAGMPILMQGLIKFSYRDMTSSLIRWLEGSYLLY
                     LLDRCWQWEKPEEGLLERVLYFWEIILKKGEDVYSSFLRFKLDLAFYLGIAPHLESCV
                     LCNRDVEGEAYWSNQEGGVICPSCASKVPEKVVFPQDLRAILRYLSSPGGNLSSLKLS
                     FFQFEELDKILSDYLSYEVGDRVMNWHDFRSLYQGAVFSS"
     gene            6991..7878
                     /gene="glyQ"
                     /locus_tag="PKN31_03075"
     CDS             6991..7878
                     /gene="glyQ"
                     /locus_tag="PKN31_03075"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOA98945.1"
                     /translation="MYFQDIILTLSDYWKKKGCVVVQPYDVEVGAGTMNPATFLRVLG
                     PEPWRVAYCEPSRRPADGRYGENPNRLYEHYQFQVIIKPSPSDIQDLYLESLFHLGIK
                     SEEHDIRFVEDNWESPTLGAWGLGWEVWLDGMEVTQFTYFQQAGGIDLKPIAVEITYG
                     LERMAMYLQEKENVFEVEWNEYLTYGDIRWQEEREHSISSFDESNPPALFTLFGLYEG
                     EAKRLLDKRLILPAYDYVLKCSHIFNLLDACGEISVMERNQYMGKIRQLANRCAQLYL
                     EERKALGFPFSSKIKTAAS"
     gene            7907..10006
                     /gene="glyS"
                     /locus_tag="PKN31_03080"
     CDS             7907..10006
                     /gene="glyS"
                     /locus_tag="PKN31_03080"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOA98946.1"
                     /translation="MTQEKDLLLEIGMEELPISLISDIFQEWETKFTRGFREARIDYK
                     DLEVVGTPRRMTVYLRGVAERQNPVLKEIKGPAKKVGISETGEFLEPAHAFARSQGID
                     LSDLVVKETERGVYIFGVKKEEGRLVEEILPLLLPRIISSLEFPRSMLWGEGQFRFVR
                     PLRWICALWGEKEIVFEIAGVVSSRFTRGHRFLAPQPVELKEPSQYFTSLREAKVIVC
                     QEERRKKIEEALSREEKLIGGHWLKDESLIQEVNFLVEYPDAVTGRFDSQYLKLPHRV
                     LTTVMKHHQKYFACVDDRGELLPFFFIVLNRPREGGEKIVKGNERVLKARLEDALFFF
                     EEDRKKPLRDRVEALDGIIFQESLGTMKQKVERLEKLARYLGEELGFDSGRIKRAEEA
                     AHLSKADLACEMVKEFPELQGYMGRVYALLDGEEEEVAIALEEQYYPLPGEEIYPSTV
                     AGMILSLVDKMDNIVSSFSLGRIPSGSQDPWGLRRMAQGIVNIILHCQWYLSLSPWVE
                     KNLHLLKEQGLGGENEGIKAEVVEFLFNRLRSRLLSSGFNYSVVNAVLNTSVDDLYEA
                     FKKIEVLEKLRRERRSFLSEIVTGFTRANNISKEFSGNGDLREDLLQEELEIELYKNL
                     QKVEQDFNQAVAGGNYFAAFEIFYSLLPLLNQFFDQVLVMCEEKDLRENRLNLMKKVV
                     NLWKDVADLSQVVIEGE"
     gene            10018..10869
                     /locus_tag="PKN31_03085"
     CDS             10018..10869
                     /locus_tag="PKN31_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HOA98947.1"
                     /translation="MNSAYKVSDDFNIFVISDGTGKTATSMVQAALLQFDLPRVNIVR
                     FARIRSREEIYNILDMVQPKKDIIAYTVVNEELAQALRVESLNRGILALDLLGPVVEA
                     LKKVSSRQPRGVPGLLRRQEEGLSPKIEALEHAWKESSGFSVSNLEEADVVILGVWYP
                     RREEGILMLAERGIRAAFLILDPALPLFPNLEETVAHSVDKPVIGLQMDVDYLTRVRE
                     ERVRALGLDRLLYKAEKEVVKEELDFAQRVYDKLGCQVIDVTGLSSRDLVNAIMSQIK
                     KKREEMR"
     gene            10866..13529
                     /gene="ppdK"
                     /locus_tag="PKN31_03090"
     CDS             10866..13529
                     /gene="ppdK"
                     /locus_tag="PKN31_03090"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOA98948.1"
                     /translation="MKKKYVYFFGEGDASMKLLLGGKGANLAEMTRIGLPVPPGFTIT
                     TEACTHYYKNNQTWPEGLAEEVEENLKRLEEETGKKFGDSQNPLLVSVRSGAPASMPG
                     MMDTILNLGLNDQVVEGLAQLTGDRRFAYDCYRRFIQMFGGVVMGIGHDEFEAILSEV
                     KREVGAKSDADLDANALIKVVERYKELYKKATGEDFPQDPKEQLYRAVNAVFESWNNK
                     RAITYRRLHNIPEDWGTAVNIQVMVFGNMGFDSGTGVGFTRNPSTGEKEYYGEYLLNA
                     QGEDVVAGIRTPKPIIEMQKDLPEVFEELKKIYDILEKHYRDMQDFEFTIEKGKLYIL
                     QTRTGKRTAQAAIKIAVDMVKEGLIDEKTAVRRVEPEQIDQLLHPRIDRSQPLNVIAK
                     GLPASPGAAYGKVVFDADEAEQLANNGERVILVSPETTPDDIHGMAAAQGVLTSRGGM
                     TSHAAVVARGMGKPCVAGCEAIKIDLDKRIFSVNGVVVKEGDYITIDGSTGEVILGQV
                     ETIPPQLSPDFQTLLSWADKFRRLGVRANADTPTDARKALEFGAEGIGLCRTEHMFMA
                     QDRLPWVQKMIMAQSPEERKEPLSKIGPMQKGDFKEIFRVMEGKPVTIRLIDPPLHEF
                     LPSLEELLVEVTTLRVKGVTGPELEEKEKLLKVVQGMHENNPMLGLRGCRVGILYPDI
                     VEMQVRAIIEAACELSQEGVKVIPEIMIPLVGHRNEIMILKEKLDEVAREVIDNYKAD
                     VNYSFGTMIEVPRAALVADQIAEYAEFFSFGTNDLTQMTFGYSRDDAEGKFLFFYEEN
                     GILEENPFQVIDQEGVGQLMRMGVEKGRSSRPDLKCGICGEHGGEPESVKFCHEIGLD
                     YVSCSPFRVPIARLAAAHAALGSEEGKKFNV"
     gene            13666..14739
                     /locus_tag="PKN31_03095"
     CDS             13666..14739
                     /locus_tag="PKN31_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOA98949.1"
                     /translation="MAREIADIKKFWETKEREILSPFATLSTSSRGREKEEPECELRN
                     CFQRDRDRIIHSKSFRRLKHKSQVFLSPEGDHYRTRLTHTLEVAQIARTVARALCLQE
                     DLVEAISLGHDLGHTPFGHAGEEVLNEVLQPQGFRHNEQSLRVVDFLEKDGEGLNLTW
                     EVRDGIAKHSKTLAEDLVFSVEELPSTLEGQVVRWADVVAYVNHDIDDALRGKVITED
                     ELPACALQVLGNTHRERIDRMVKDLILSSFDQPVVKMSDEVREATEILRRFLFNRVYL
                     SEEQKVERDKAKRLVKELYFFFLQNPDILGKEYKLYQKGASDLEQSVCDFISGMTDRY
                     ALYIYQKYFIPSSWPGEGRRNTT"
BASE COUNT         4252 a         2631 c         3938 g         4039 t
ORIGIN      
        1 aggtgataga accattcagg aatgagggac ttgggcggtc attgcgagcc tcgtattctc
       61 gtatggcgtg gcaatcccgt ttttctttgc cgaggagaat ccaaaaagag atcgccacgc
      121 atggcaaaat acgccatgct cgcgatgaca agaaaaagac accctcaccc taaccctctc
      181 ccgtcgaggg cgagggaaaa tatggagagt ctcctgaaga ggatgagggg aagcgagatc
      241 gccacctcaa tcgctcctcg cgatgacaga tgataaaggg gggttatgag gggacagcgc
      301 tcccttatag tcgatactat agttctcttt attgtttctt tctcatactg acattactat
      361 aggataacta cagccagcca ggtagtttat ttgacacttt tcttttaatt tcttataatg
      421 gttttaactt ggatttaggg aggaataagg gtgactgaaa tcaaagtcgg taagggagaa
      481 agcatagacg aagctttgcg ccggtttaag aaaaagtgcc agcgtaatgg agtaatttcg
      541 gagatgaaac gcagggagca ttatgagaaa ccgagtgaaa agaaaagaaa gaaggctcaa
      601 gcagctgccc gaagaaaaag aagaggatga aagaggtatc gtctttaaaa gttcagattg
      661 aagagcagat gaaagcagca ctgaaggctc acgaccagct gaggctggat actttgaggt
      721 tgctcctttc agagataaaa aataaagaga ttgaaaaaag gggcaatctg gaagaggggg
      781 aaatagttgc cttaatccgg aaggagatta aaaagaggca agaaagtgaa gaatattttg
      841 aaaaaggaaa tcgttccgag cttttagaaa aggcagagaa agagatagcc attctgcaag
      901 attttcttcc tcctcaactt tcagaggagg aagtggagcg agttataaat gatgtattag
      961 ccagctggcc tggagaagca tctcttttcc cggtgatgaa ggaagtgatg tctcgccttc
     1021 agggcagggc agggggggaa gtgatttcta aagtggtatg tcaaaagata aacgtggggt
     1081 gatagaaaaa cttgtctcaa gggattgacc tgaaagatag agaagtgcgt ttctctttag
     1141 aaagtgtgga aaacattaaa gcgctttttg gctatctgga tagtaaaatc agagtcgttg
     1201 aaagtgtgtt taaggtagag gtaactatag aggaagagga tctagtgatc agaactaaaa
     1261 gtgagagtgg agaggtatct ttagcccgag attttttgca agaactttct tctttttctc
     1321 ctccagaaga attgagttct gatgacctgc aaaaaatggc tctcagtttc aaagaaaaca
     1381 aaaaagcgtg gtggaatgat aaagtagaac ccactattct gactactgct tatcataaac
     1441 ctgtccgggc taaaagtccg ggacagatga aatacgttca gacggtaaga aatgcggata
     1501 taactttctg catcggtccg gccgggacgg ggaaaactta tttagccatg gctatggcct
     1561 gtgctgccct gcaaaccaag aaagtgagca gaattgtcct ggtaaggccg gcggtagaag
     1621 caggagaaag cctgggatat ctacctggag acctgaagga gaaaattgaa ccctatctac
     1681 gacctcttta tgatgccctc tatgaaatgc tctcccctga taaatttcag aagtacagcg
     1741 aaaaaggagt gattgaggtg gctcctcttg cctatatgag agggaggacg ctcaatgatt
     1801 ccttcatagt tctggacgaa gcacagaata ccactcctga acagatgaaa atgtttttga
     1861 cccgcatggg cttcggttcc aaggtggtgg tgaccggcga tatcacccag attgaccttc
     1921 ccagtggtaa aaaatcgggg ttagtggtgg tgcaagaaat ccttaaggac attaagggaa
     1981 tagaatttgt ttatttgaac gaaaaagatg tggtcagaca tcagttagtc cagaagatta
     2041 ttctggctta tgaaaaattt gaggaaaaag ctctctctcg ctaagtctca tcttctttct
     2101 tttcgcttcc tgaattttct cctttatgga ataatgcttt tctttctctt ctggttttct
     2161 tcctggggag aggaatggaa aataaaagag ggagaagtgg tcagtcgaga catcattgct
     2221 cctcgctcgg tggagattat cgatattccc agcactgcag aagcacaaaa gaggcttctc
     2281 tctgctattc ctcctgttta tcgtgtcgac cctcaaacgc tggataaaat tgaacaagag
     2341 ttagaagaat tttttaaatc tctggaagaa gctcgtccta cctactctct tttatctacc
     2401 gattttaagg atacctttag taagaagtgg agaattaccc cgggggtgtt ggagtggctt
     2461 attgcctccc ctcaagagga atatgagaga gtcaaaggag ttacttttga tataatcaac
     2521 cgctatatgg ccaaccctat ccgggaagaa gaattagaag agaagatttt agaggcctat
     2581 agtgagctgg aaagaatgga tctgggagaa agcgaaataa gagtggcaga agccctggtt
     2641 ttccgttttc tccggcccac tgcagtggtg gatagcgagg gcacacaaag agaacgcttg
     2701 aaagctctgg aatctttgca accggtaaag agaattatta agcgagggga agtgatttta
     2761 agaaaaggag aaatagctac taaagaggac atagaaattt taagagccat gggcttcggg
     2821 ggagaaggag atcagtggtt ccagctttta gcctgggctc tggtaataac tggagcggtg
     2881 gtggcagaat atttctatat ccgaaaattt gtcccttctc ttttggagga cgattctctg
     2941 ctcctgttgc gtttaatagc ggtagggggc attgtagcca tgaatacact gacttttcgc
     3001 ctttcctcct tttttataat catcagtgct cttcctctta ttcttttgcc tcttttaggt
     3061 gaagattatg ctctggggga atccttgatt ctttttcctc ttattctttt gggggacagg
     3121 gtggatttta ttcagggatt ttatatctat gttaatttat tcttgccact tttctggtta
     3181 agcaaaatct ctaaaacagg agacctggta aaagcgggaa tgatgatggc ctttaccaat
     3241 atgggtttga gctttgtttt tggtttgcag gagggtaaag agttaaccaa agttttagct
     3301 gatgcttttt atggatgggg aggaggaatg ggaggagcag taatcgctct gggtggaatt
     3361 actcttttgg agagtatttt tcacataact tccgattttc ggttgatgga attgctaaat
     3421 cctactcatc ctcttttgaa aagacttctc atggaagctc ccggcactta tagccacagc
     3481 ttgatggtgg ctaatctcgc tgaagctgca gcggagagaa ttggagccaa tcctttttta
     3541 gtgagggttg gagcttatta tcatgatgta ggtaagataa aaaggcctta cttttttatt
     3601 gagaaccaga tggaggggca taatgcccac aatcgtcttt cccctaactt gagctcttta
     3661 attatccaga gtcatgttaa agacggggtg gaaatagcca ggcaatacaa tttgcccccc
     3721 gaaattcagg aaatcatcgc ttgccatcac gggaccacta taatacgcta tttttacgat
     3781 aaggcggtaa aggaaagaaa agatgaggtg agggaagaag agtttcgcta tcctggacct
     3841 ctaccgcgta ctgttgaaca agcactggtg tttttagctg atagtgtgga agcagcggtg
     3901 cgcagtgcta ctcgtcccac tcccagtcgg gtggatgcca tagttaacgg tattttgcaa
     3961 aaatatttgc aggagggaca gctggatgaa tctcctctca ctatgagaga tatccggcaa
     4021 attgctcaag ctttcgtggt ggttttgaac ggaatgattc actcccggat tccctattat
     4081 ccggaagaag aggcattaaa aatagtcagg gggaaagata taaaagatga gccgggtaga
     4141 aatagtaaat agaaccaggg aaagcttttt aaccagaaaa aaggtgcaga aaatagtaga
     4201 cttcgtttta aaagaggaag ggaaagactt tccggggtgt ttgagtatag cggcagtgga
     4261 tgaggaggag attcgaagcc tgaagaaaag gttttttcac caggatgtct gtaccgatgt
     4321 tatatccttt ttttacggat tttctgatga agatgatatc tggggagaga taataatttg
     4381 tcctgctgtt gtcaagcaag aagcacatgt ccggggagag agcgaagaga gagcgttgag
     4441 catcgtttta atccatggct tgcttcatct tctgggatac gaagacgaag atgctgaaaa
     4501 ggccagagta atggaagaaa gacaagaagc tctgtatcgg cttttagaag aggaagagga
     4561 aagagaaaag ttgataaaaa gagctatcaa ggtgagagaa tatgcctatg ctccttattc
     4621 ttcttttccg gtgggggcgg cgctgctggg atatgacggg gatattttta ccggttgtaa
     4681 tgtggaaaat gcctccctgg gtttaaccat ttgtgccgaa agggtggcga tttttaaggc
     4741 tatatcacag ggggtaagag aatttaagag aatagccatc attgctgacc cttcttctta
     4801 ttgttttccc tgcggagcct gccggcaggt tctttatgaa tttgctcctc aactggaggt
     4861 aatagcggct aaaagtgata gagattatga gatagtttct cttcactctc ttttaccgcg
     4921 ggcttttttt ctgagaaatg acgatagaaa ggagtaaata acttggcttt ccgctctggt
     4981 tttgttacca tatgggggca acccaatgta ggaaaatcta cttttttaaa ttacgttttg
     5041 ggacgcaagg tagctattgt ttcttccaag cctcaaacta cccgtcaggt tttaaaggga
     5101 attctcaacc tccctgatgc ccaggtggtt tttttagata ctccgggttt gcattctccc
     5161 cgggatgaat ttggtaaatg gatggttcag gaggctgaaa aagctctgga tggtgttgat
     5221 gtgcttctct atatgattgc tcccgataac agggaagaag aggatagaaa atatctgaat
     5281 agtttacatt cttttaccgg cccggttttt ttggttctta acaaaaaaga cctggtgcaa
     5341 gaagaagaag cccggaaaat tctttcccgg ttttctaatc ttttcccctt ccaagggaaa
     5401 gcggcagttt cttctatcac tggagagggg atttctgagt tgctttctga gatagtggaa
     5461 gctcttcctc cggggcctcc ttattatgga gaggatgtta tctctgatat ctgggaaaaa
     5521 gatatagtgg cagaaataat aagggagaaa atctggtggt atacttatca ggaagtccct
     5581 tacggggtag aggtgagggt ggaggagttt aaagaccggg gtgatattct ctatatcaga
     5641 gctattatat atgtggagag ggattcccat aaaaaaattg tcattggtca ggaaggcaaa
     5701 atgttaaaaa aaattggaga agaggccaga aaagaaatag aatttaactg gggcaaaaaa
     5761 gtttatctcg atttgtgggt taaagtggag aagaactgga ggaaaagacc cgaggttctg
     5821 ggaagatggg gatacaggac taagtgagag agagatttaa tcgttactgg aaagacgagg
     5881 gaatagtgat tcgctctact aaccttggag agcgagaccg tctggttacc cttttcactc
     5941 agaagagagg aaaaattaga gctttggcaa aaggagctcg aaaagtggga aatcgttttg
     6001 gagcttcttt agatgttttt aattattccg aatttatgtt ttattccggg gcgggaatgc
     6061 ccatactcat gcagggttta attaagtttt cttaccggga tatgacttct tcactgataa
     6121 ggtggttaga gggaagttat ttgctgtatc ttttagaccg ctgctggcag tgggaaaagc
     6181 cagaagaggg gcttttagaa agagtgctat atttctggga gataattttg aaaaaggggg
     6241 aggacgttta ttcttctttt ctccggttca aactggattt ggctttttat ttaggaatag
     6301 ctccacatct cgagagctgt gttctctgca accgagatgt ggaaggagaa gcttactgga
     6361 gcaaccagga aggtggtgta atctgtcctt cctgtgcttc aaaagtgcca gaaaaagtgg
     6421 ttttccctca ggatttgagg gctatactca gatatctttc ctctccgggg ggaaatttat
     6481 cgtcgctgaa gctcagtttt tttcagtttg aggaactgga taaaattctt tctgactatt
     6541 tgagctatga agtgggagat agagttatga actggcatga ttttcgatct ctttatcaag
     6601 gagcggtttt ttccagttga caggattttc taaaattgct atattttagg cttggtgtgt
     6661 tcggggagga ataggataat ttatactcat aaaaaagaca tccagctaag tatcaggcag
     6721 tggctatgat gaaagataag gcgttgttaa ctaaaagttt agcagcaaaa aatctcggaa
     6781 tactaaaaac tctgtcctcc ctgccaccat tgagcctctt ttcaggaaat aatgagtaaa
     6841 aacaagtact tttgagcttt gctctttggg gggagagcga ggaggctgtc ttaaaaccgc
     6901 gggctgagcg aaggcagctt gaagaaggcg ggataaaagt atctcagcaa actatgccgg
     6961 ttgtggtata gtttagttag ccaggaagcc gtgtattttc aagacatcat tctgaccctc
     7021 agtgattatt ggaaaaaaaa gggttgtgtt gttgtccaac cttatgatgt ggaagtggga
     7081 gcaggcacta tgaatccggc tactttcttg cgagttctgg gaccggagcc ctggagagtg
     7141 gcttactgtg aaccttctcg ccgtcctgcc gatggtcgat acggagaaaa ccctaaccgg
     7201 ctttatgaac attatcaatt tcaggttatt attaaacctt caccttctga tattcaggat
     7261 ttatatttag aaagcctttt ccacctgggc attaagtcag aagaacacga tatccgcttt
     7321 gtggaggata actgggaatc tcccaccctg ggagcctggg gtctgggctg ggaagtctgg
     7381 ttagacggaa tggaagttac ccagtttacc tattttcagc aagccggagg aatagactta
     7441 aagcccattg ctgtggagat aacttatggt ttagaaagaa tggctatgta cctgcaggaa
     7501 aaggaaaatg tattcgaagt agagtggaat gaatatctga cctatggaga tatccgctgg
     7561 caagaggaga gagagcactc tatatccagc tttgatgaaa gcaatcctcc tgcacttttt
     7621 accctcttcg ggctttatga aggagaagct aaaaggttgc tggataaaag gttgattctc
     7681 ccagcttatg attacgtttt gaaatgttcc catattttta acctattaga tgcttgtgga
     7741 gaaataagcg ttatggaaag gaaccagtat atggggaaaa taaggcaatt agccaatcgt
     7801 tgtgctcaac tgtatctcga ggaaagaaaa gctctggggt ttcccttttc ttccaagata
     7861 aaaactgctg catcttagtg gctctggacc ggagaggtaa ggataaatga cacaagaaaa
     7921 agaccttttg ttagagatag ggatggaaga gctgcctatc agtttaatat ctgacatttt
     7981 tcaggaatgg gaaactaaat tcacccgggg ctttcgggaa gcccgcatag attacaaaga
     8041 cctggaagtg gtgggaaccc cccggcggat gaccgtttat ctgcggggcg tagcagaaag
     8101 gcagaaccct gttcttaaag agataaaagg tccggctaaa aaagtaggta tttctgaaac
     8161 aggagagttt ctggagcctg ctcatgcctt tgctcgctct cagggtattg atttatccga
     8221 cctcgtggta aaggagactg aaaggggggt ttatattttc ggggtgaaaa aagaagaagg
     8281 aagactggtg gaagaaattt taccccttct ccttccccgg attatttcct ctctggaatt
     8341 tcctcgttcc atgctctggg gagaaggaca attccgattt gttcgtcccc ttcgctggat
     8401 ttgtgctctg tggggggaga aagagatagt tttcgaaata gcgggagtgg tatcttcccg
     8461 ttttacccgg ggacatcgct ttcttgcgcc acaacctgtg gaattaaaag aaccctctca
     8521 gtattttact tcgctgcggg aagctaaagt tatagtttgt caggaagaaa gaaggaagaa
     8581 aatcgaggaa gctcttagca gggaagaaaa actcatcggg ggccactggc tgaaagatga
     8641 gagcttgatc caggaagtaa attttttagt ggaatatccc gatgccgtaa ccggtcggtt
     8701 tgattctcag tatctgaagc ttccccatcg agtgcttacc acagttatga agcatcacca
     8761 gaagtatttt gcctgtgtag atgaccgggg ggaactgctg ccctttttct ttatagtttt
     8821 aaatcgaccc cgggaagggg gagaaaaaat agtaaaggga aatgagcgag tacttaaggc
     8881 ccggctggaa gatgcgcttt ttttctttga ggaagacagg aaaaagcccc tgagggatag
     8941 agtagaggct ctcgatggca taatctttca agagtctctc gggaccatga aacaaaaagt
     9001 agagcgatta gaaaaactgg cccgttacct gggagaggag ttgggctttg actcaggaag
     9061 gataaaacgg gccgaggaag ctgctcatct atctaaggca gatctcgcct gtgagatggt
     9121 aaaggaattt cctgaactac agggttatat gggtagagtc tatgctcttt tagatgggga
     9181 ggaagaggaa gtggcaatag ctctggaaga gcaatattat cccctaccag gggaggaaat
     9241 ctatcccagc acagtagcgg ggatgattct ctctttggtg gataaaatgg ataatattgt
     9301 ttccagtttt tccctgggga gaatccccag cggttctcaa gacccctggg ggttgaggag
     9361 aatggctcaa ggcattgtga atataatatt acactgccag tggtatctgt cattatctcc
     9421 ctgggtagag aaaaatcttc accttttaaa agagcagggt ctgggagggg aaaatgaggg
     9481 gataaaagcg gaagtggtag aatttctgtt taatcgcctt cgttctcgtc ttctgagctc
     9541 cggctttaat tattcggtgg ttaatgcagt tttgaacact tctgttgatg acctctatga
     9601 ggcttttaaa aaaatagagg ttctggaaaa gctgagaaga gagagaaggt cgtttttatc
     9661 tgagatagtc accggcttta ctcgagctaa caatatcagc aaagagttct ccggtaatgg
     9721 agatttgcga gaagacctct tacaagagga gttagaaata gaattatata aaaatctcca
     9781 gaaagtagag caagatttta atcaggcggt ggcgggggga aattatttcg ctgcttttga
     9841 aatattttat tctcttttgc ccttacttaa tcaatttttt gaccaggttt tagtgatgtg
     9901 tgaagaaaaa gatttaagag aaaatcgctt aaatttgatg aaaaaagtgg taaacttgtg
     9961 gaaggatgta gctgacctgt ctcaggtggt aatagaggga gaataaagtt gaaagaaatg
    10021 aattcggctt ataaagtgag cgatgatttc aatatttttg tgatttctga cggaacgggt
    10081 aaaactgcga catcaatggt acaggctgct cttttgcagt ttgacctgcc tcgagttaac
    10141 attgtgcgtt ttgcgcgtat tcgtagtcga gaggaaatat ataacattct ggatatggtg
    10201 caacccaaaa aagatatcat agcttatacg gtggtaaatg aagaattagc tcaggctcta
    10261 cgagtggaat cacttaatcg agggatactg gctctggatc ttctgggtcc ggtggtagaa
    10321 gcgctgaaaa aagtcagttc tcgccagccc cggggagtgc ctggtctcct ccgtcgtcag
    10381 gaagaagggt tatcccctaa aatagaagct ctggagcatg cctggaaaga aagtagcggt
    10441 ttcagtgttt ctaatctgga agaggctgat gtggttattt taggagtgtg gtatcctcgc
    10501 agagaagaag ggatactgat gctggcagaa agagggatca gggcagcatt tttgatatta
    10561 gacccggctt tacctctttt ccccaacctg gaagagacag tggctcactc tgtggataag
    10621 ccggtgattg gtctccagat ggatgttgat tacctgaccc gggtgaggga agaaagagtc
    10681 agggctctgg gtctggatcg cctcttgtat aaagctgaaa aagaggtggt aaaggaagaa
    10741 ctggattttg ctcagagagt ttatgataaa ctgggttgcc aggtgataga tgtaactggt
    10801 ttgagcagta gagatctggt aaatgcaatc atgagtcaaa ttaaaaaaaa gagggaggaa
    10861 atgcgatgaa gaagaaatac gtctattttt ttggcgaagg tgacgcctca atgaaattac
    10921 ttctgggtgg taaaggagca aacctggcgg agatgacccg tattggttta ccggtgcctc
    10981 caggttttac catcaccacg gaagcctgca ctcattacta taagaataac cagacctggc
    11041 cagagggtct ggctgaagag gtagaggaga atttaaaacg cctggaggag gaaaccggta
    11101 agaaatttgg tgattcccag aatcctcttt tagtttctgt ccgctctggt gctccagctt
    11161 ctatgccagg aatgatggat accattctta acttgggtct taatgaccag gtggtagaag
    11221 gcctggctca actcaccggt gatcgtcgct ttgcctacga ctgttatcgt cgtttcattc
    11281 agatgttcgg tggcgtggta atgggaatag ggcacgatga attcgaggcc attttgagtg
    11341 aggtcaaaag agaggtaggg gccaaatcag atgccgatct ggatgctaat gctttaataa
    11401 aggtggttga gagatacaaa gagctttata aaaaagcaac cggagaagat tttcctcaag
    11461 accccaaaga gcaattatat agagctgtta atgccgtgtt tgaatcctgg aataataaaa
    11521 gagctatcac ttaccggagg ctccataaca ttcctgaaga ttggggcaca gcagtaaata
    11581 ttcaggtgat ggttttcggt aacatgggat ttgattcggg aacaggggtg ggctttactc
    11641 gtaacccctc aaccggagag aaagaatatt acggcgaata tcttttgaat gcccagggag
    11701 aagacgtggt ggccggtatc cggactccca aacccatcat agagatgcaa aaagaccttc
    11761 ctgaagtttt tgaagagctg aagaaaatct atgacatatt agaaaagcac tacagagata
    11821 tgcaggactt tgagtttacc atcgaaaaag gtaaacttta cattttgcag actcgaacgg
    11881 ggaagagaac tgctcaggca gcgattaaga ttgccgtgga catggtaaaa gaaggactga
    11941 ttgatgaaaa gacagcggta cggagagtgg agcccgaaca aatcgaccag ctgctccatc
    12001 ctcgtattga ccgcagccag cctttgaatg tgatagctaa aggtttacct gcttctcctg
    12061 gtgcagctta tgggaaagta gtttttgatg ctgatgaggc agagcagtta gctaataatg
    12121 gtgagagagt tattctggtc agcccggaaa ctactcctga tgacattcat ggaatggcgg
    12181 cagcacaggg agttttgacc agcagaggtg gaatgaccag tcacgcggca gtggtggctc
    12241 gaggtatggg taaaccctgt gtggcgggtt gtgaggcgat taagattgat ctggataagc
    12301 gaattttttc ggtcaatgga gtggtggtaa aagaaggcga ttatatcacc attgatggga
    12361 gcaccggaga agtgatttta gggcaggtgg aaaccattcc tcctcaattg tctcctgatt
    12421 ttcagactct cctttcctgg gcggataaat tccgtcgttt aggagtgcga gccaatgctg
    12481 ataccccgac agatgctcga aaagctctgg aatttggagc ggagggaatc ggtctttgcc
    12541 gaaccgagca catgtttatg gctcaagatc gcttgccctg ggtacaaaag atgatcatgg
    12601 ctcagagtcc cgaggaaaga aaagaaccct tatccaagat tggtccgatg cagaaagggg
    12661 acttcaagga aatattccgg gttatggaag gcaaaccagt taccattcgt ttgattgacc
    12721 ctcctttgca tgagttttta cccagcttag aagagctctt agtagaggtg actactttac
    12781 gggttaaggg agtaaccggt ccagaactgg aagaaaaaga gaaattgcta aaagtagttc
    12841 agggaatgca cgagaataac cccatgctgg gtttgcgggg ttgccgagtg ggcattctct
    12901 atcctgacat cgtggagatg caggtgaggg ctattataga ggcagcttgc gagctatctc
    12961 aggaaggagt taaggttatt cccgagatca tgattcctct ggttggccat cgcaatgaga
    13021 ttatgatttt aaaagaaaaa ctggatgagg tggcacggga ggttatcgat aattataaag
    13081 cagatgttaa ttacagcttt ggtactatga ttgaggttcc ccgagcagca ctggtggcgg
    13141 accagattgc tgaatatgcc gagtttttct cctttggaac caatgacctt actcagatga
    13201 cttttggtta tagccgggat gatgcggaag gtaagttttt gttcttctat gaggagaacg
    13261 gtatactgga ggaaaacccc ttccaggtta tagaccagga aggagtgggg caactgatgc
    13321 ggatgggagt agaaaaaggt cgtagcagtc gtccggatct aaaatgtgga atctgtggag
    13381 aacacggagg agagccagaa tcggtgaagt tctgccacga aattggtctg gattatgtca
    13441 gctgttctcc tttccgtgta cctattgctc gcctggctgc tgctcatgct gctttaggga
    13501 gtgaagaagg aaagaaattc aacgtttgag tttttctccc ctgccccgag ggggcagggg
    13561 agagctgtat tggaactccc gggaggaggt atcttcccgg gtaggcatta tacaagtgag
    13621 aagacagcag gcctttagag ggtaaataca ggagtgggtg aggcgatggc tagggaaatc
    13681 gcagacatta agaaattctg ggaaacaaaa gaaagagaaa tattatcgcc atttgctact
    13741 ttaagcacca gtagtcgggg aagagaaaag gaagagccag aatgtgagct acggaattgt
    13801 tttcagagag atcgagatcg catcattcac tccaaatctt tccggcgcct gaaacacaaa
    13861 agtcaggttt ttttaagccc ggaaggcgat cactatcgta ctcgtctgac tcatactctg
    13921 gaagtagcac aaattgcccg cactgtggct cgggctttat gtctgcagga agatttagtg
    13981 gaagcaatca gcttaggtca cgacctgggg catacccctt ttggccatgc gggagaagaa
    14041 gtgcttaacg aagttcttca acctcaaggt ttcaggcata atgagcagag tttaagggtg
    14101 gtggattttc tggaaaagga cggagagggg ctcaatctca cctgggaagt aagagatgga
    14161 attgctaaac actctaaaac tctggctgaa gacctggttt ttagtgtaga ggagcttccc
    14221 tctactttag agggtcaagt agtgcgctgg gctgatgtgg tggcttacgt taaccatgat
    14281 atcgatgatg ccctgcgagg taaagttata accgaagatg aacttcctgc ttgcgccttg
    14341 caagttttag gcaataccca tcgagagcgg atagaccgaa tggttaaaga tttgatttta
    14401 agtagctttg accagccagt ggtgaaaatg agcgatgaag tacgagaagc caccgagatt
    14461 ttgcgccgat tcctttttaa tagagtgtat ttgagtgagg aacaaaaggt agaaagagat
    14521 aaggctaaaa ggctggttaa agagttatat ttcttttttc tgcagaatcc agatatcctg
    14581 ggaaaggagt ataaacttta ccagaaggga gcatctgatt tagaacaatc ggtttgtgat
    14641 tttatttcag gaatgacgga tagatatgct ctatacattt atcaaaaata cttcattccc
    14701 tcttcctggc cgggagaggg aagaaggaat actacttgaa taaatagaag gaggtgtatt
    14761 atgggagtcg caatttttac tctgattgct ggtttaatcg ctgtaattta tgctgttttt
    14821 ctcgttcgcc ggatctattc ttttgacccg ggaaatgaga
//
LOCUS       DAOPSS010000029        14762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_3450_length_14762_cov_7.554090, whole genome shotgun sequence.
ACCESSION   DAOPSS010000029 DAOPSS010000000
VERSION     DAOPSS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 14762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14762
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_14762_cov_7.554090"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            532..2409
                     /locus_tag="PKN31_03100"
     CDS             532..2409
                     /locus_tag="PKN31_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOA98950.1"
                     /translation="MTLKTKILSGFIIMIVLVAIVGCIGFFATSRLNDSLYKVYAVRM
                     PALDYLIEADRDLQQLLVAERSMLSAPSDSEIFKRLLADYEENFNQSIERFNKYKELA
                     QAPEELQLISGYEEVRKEWETVSKQVINYLTEGGEEERNLAIELSLGKAAEKFEETRE
                     YLNQLTDVILNLTEEENLSAQKARTMSLSLITWTIIISIVLGLTFALLLTRSIRKPLQ
                     EISSAAEKVASGNLQVEVKKLTVKDELGKLSNSFAEMVNSLRNLVHNTLNSSSLLASS
                     SEELASSVSEASRATAEIAQTISQVAEGSIRQSEELQKIAEEGDTMAKKAQIIVNTSD
                     DNLRLLEETIKNLEENSHILSTISKMNNSVSDQSKITQEKAQNGKKLLIDLVASVEDI
                     AEVSQEVAQSVELLTERSQEIGKIVDLITGIAEQTNLLALNAAIEAARAGDAGRGFAV
                     VAEEVRKLAEESAQAAEQIGALISEIQKNTGETSEAMQRAKNQVETGSKQSEKVSQSF
                     EDIIAATGEVANSIGSLTRTIGELETNQQATAENSQSLVKHTEDTLHDLRSISESISK
                     LHETITTVASIAEENAASSEEISASVEEQNAAMEEINSMAASLSKLAEELQEVVNNFT
                     V"
     gene            2824..3714
                     /locus_tag="PKN31_03105"
     CDS             2824..3714
                     /locus_tag="PKN31_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOA98951.1"
                     /translation="MAIYHIGTCSWTEKTLIEDGSFYPPQLKNPAERLNFYAQHFNTV
                     EVDSTFYAFPAERNARLWVERTPSSFLFNIKSFSLFTFHKTSLSVIPQFLKEELPERL
                     QKRPHFYLAEVPEEWLKIALSAFLSAINPLQEENKLGYILFQFPPWIEKSRETMEYLS
                     WLRENIKGTLAIEFRHHSWLSEENRDDTFCFLRKERLAYTCVDEPQLPWTVPPIVEVT
                     TPELITRFHGRNKPAWEKKGAPVEEKFAYLYQKEELNRWKEVVKKKSPGAERVFLMFN
                     NCFRDYAIRNAKQMKLLLAE"
     gene            3724..4344
                     /locus_tag="PKN31_03110"
     CDS             3724..4344
                     /locus_tag="PKN31_03110"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOA98952.1"
                     /translation="MEIIDISLPLSEETPTYKGRKEKKPRLERIRTLSQGANETRLTL
                     ETHTGTHVDAPLHMLSPANSIDQLPLSSFLGPALVVEIRGVEAIAAEHLSPFQQFLSP
                     RQFLILKTDNSFSDLSRENFVYLTVSGASYLAQQGAKGVGIDSLGIERDQPGHPTHRL
                     LLEKGILIFEGLYLREVEPGFYYFLSFPLKIKEGDGSPTRAVLFRM"
     gene            4346..5731
                     /locus_tag="PKN31_03115"
     CDS             4346..5731
                     /locus_tag="PKN31_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98953.1"
                     /translation="MKTLIFFGATGNLFTQKIFPALSSLFYHYEIRDLALIALGRRFS
                     KEGDYRRFLQKIHQREEKELLERVFYLPGDLENPEIPSKLSQLINKTGKEEAIFYLSL
                     PPSLYSSALKTIEKTLSLSSPSTKKVALEKPFGSSEKDFEELEKALKKIFREEEIFYV
                     DHYLGKATVQNLIILKAENLFIEKLLSRSFVKEVRIAVSEKEGVGERKKFYEETGAIK
                     DVFQNHLLQLLTLLAIDIPPLCEEDRKECQRFLENVGQAKSHLLTEIQLPPPEAIYLG
                     QYQNYQEEVENPHSRIETLVYLPLYISSERWQGVPFRMLTGKRMAERESFLEVVFHSH
                     FPEDNLLRIEIQPEERIDLLIRVKTPGMGLYSSPTRLNFTYSGSFGANTPEAYEKIIY
                     DLLAGDRTLFPDSTFIRHAWQIVDRLVSIIHKERIKPHLYPVDTLTPGDFWSSPPQIT
                     ISRKTSHDYRG"
     gene            complement(6339..7295)
                     /locus_tag="PKN31_03120"
     CDS             complement(6339..7295)
                     /locus_tag="PKN31_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA98954.1"
                     /translation="MSSNPFLKRRNPQLLILVIILIAIFLLFGLLTKGKIFEPRSLQA
                     IAFQLPLLGLLTLAQMSPMLTGGIDLSIVSTANLSAIVIALSMTQFELSPFWAIVLGL
                     LLSLGLGVLKGTLVAFCSIPAMIATLGMMIFIRGIALVVTKGYVIAGFPRSFLFLGDG
                     TVLGIPMPFIIFLITAAFMSLLLDRTPFGIQLYLLGSNPTATLFSGVNNRRVIFRVYL
                     LCSFLAGVASVVMTARFNAAQAGYGESYLLLTVLACVLGGVNPAGGFGKVGGLILSMV
                     VLQVVATGFNLLGLSSHLASALWGIILVSVILTNRLIFARET"
     gene            complement(7285..8289)
                     /locus_tag="PKN31_03125"
     CDS             complement(7285..8289)
                     /locus_tag="PKN31_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA98955.1"
                     /translation="MINKKIFQQTEFYLLGIIIVLSFFLTLVNPKFLTLENWFDMLRS
                     NTFLGILALGELVVLISGGIDVSFTATATIAQYIMGLIITTYYIDNVLVAFLIPIPIG
                     IALGAINGILVYYTKAHPVIITISTLNAFFGFLMFFSGGKWIYNFPPSFRNFSRVQLF
                     SLTNPQGVKYGFSIFILIWIILAIFTWVILKYFPIGRKIYAMGGNMEAARRAGFNIFR
                     VQLFVYCFMGALAGLASFVHASLNQMIQPNSLVGGELDVLAAVILGGASVFGGSGSAL
                     GAFLGVLLIAIIKNGLILMKASAYWHEVIMGAIIVVAAGISAYQRKSQAKREGEILVQ
                     "
     gene            complement(8309..9826)
                     /locus_tag="PKN31_03130"
     CDS             complement(8309..9826)
                     /locus_tag="PKN31_03130"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA98956.1"
                     /translation="MSQKIEEDQKKPYLVLQNASKRYGGVVALDGVDLTILPGEVHGL
                     VGENGSGKSTLVKIITGVVRPEKGAKVEISGQKVEIANPFDAFRLGIYVVHQDLSLFP
                     NLSVAENIFSHRYLEKAQKLVNWKEIKAEATRVMDKLGVDIDPEIEVSRLSVADQQLV
                     AISRAIAVDARFIILDEPTSSLTRKEVIRLFAFIRELKARNISILFISHRLDEVLEIS
                     DRITVLRDGKKVGTFQREEVDKKRLSFLMTGKEISSRPVEAGAYPREEEILRVENLTK
                     KGHYEKISFSLYQGEILGLIGPRGSGRTELALTLFGLNPPDEGKIYFEGKPQVISSNQ
                     VAIGLGIGYVPENRLVQGLVLDHSVENNLVITVLDQIINGMGLIDFQKKRSVAEKAVQ
                     EFNIKTESMEAPVKNLSGGNQQKVVLAKWILTSPHLLILDTPTHGVDVAAKESIYQSI
                     RELSQKNLSLIFISDEETEVLNNCDRILVMKNGQIIREFSPAEVSEEELRKIILE"
     gene            complement(9885..10910)
                     /locus_tag="PKN31_03135"
     CDS             complement(9885..10910)
                     /locus_tag="PKN31_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOA98957.1"
                     /translation="MGMKKALISFLLVLAIVFSFALGVGAEEQHEIAVVVKVAGIPWF
                     SRLEEGVKQAAKDFGVNAYMVGPTTADPAPQVQMVEDLVTRGVDAICVVPSDAKSLAP
                     VFNRAREQGIVILTHESPFETEAVDWDVETIDSVQYGKAPVDAIVEKLKETGELEKYT
                     PENPAGFVMLVGSLTVPLHNFWADTALEHAAENYPFLKELTSRLPTAESVEDSRAAVL
                     DLITTYGDQLKAVIGWGSLGPVGAAQAVTEKGMEDQIIVVGSAIPSTVAPYLRTGAVD
                     WAQLWDPRDAGYAMVYIAKQMLDGEEIVEGMEIPGMGPISIDGKVISVNQIKVMRTAE
                     EAEALGF"
     gene            complement(11508..12509)
                     /locus_tag="PKN31_03140"
     CDS             complement(11508..12509)
                     /locus_tag="PKN31_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOA98958.1"
                     /translation="MRALVLEKPAPIETDPLLLCDLPLPQISEEEVLIRIRACGVCRT
                     DLHIVEEEIPHHLLPLIPGHQIVGVVEEVGEKVKDLSPGELVGVPWLNRVCGRCRFCQ
                     DGRENLCESALFTGYDVLGGYAEYVATHQEAVYKLPEGYKDEELAPLLCGGVIGYRAY
                     RMSGVKKGDTLGIFGFGSSAHLVAQIAKIEGVEIFVFTRSPEHQLLARDLGASFAGRA
                     EDHPPHLLTSAIIFAPAGNLVKNALLYLERGGAVIMAGIYSTPLPEIPYSLLYWERSI
                     KSVANSTRQDVRELLDFAQKHHLEAKTEIFPLTEANRVLQLLKESKIKASAVLKPET"
     gene            complement(12506..13429)
                     /locus_tag="PKN31_03145"
     CDS             complement(12506..13429)
                     /locus_tag="PKN31_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOA98959.1"
                     /translation="MAKVLISGGAGFIGSHIAEAYLASGFDVVVVDNLSTGKRENLPR
                     GVTFYEGDITSLSDMEEIFFREKPDYVNHHAAQINLRRSTEEPLFDARVNILGSISLL
                     ELSVKYRVKKFIFASTGGAIYGEVKTLPAREDMPALPLSPYGVAKRSVELYLNYYHKV
                     QGLDFVALRYGNVYGPRQDPRGEAGVVAIFISRIIKGEPCIIFGDGRKTRDYVYIEDI
                     ARANLLALSASGIYNLGTGKETSVLDLVEVLKKVSGEDFPVVFGEERKGEIDRIALSS
                     EKAREALGWTPSFSLEEGIGHTWEWFKEAKQ"
     gene            complement(13582..13785)
                     /locus_tag="PKN31_03150"
     CDS             complement(13582..13785)
                     /locus_tag="PKN31_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98960.1"
                     /translation="MNPESIPQVRGAEEPFLAELGAFFKRNTKSYGKTNYKEYPGDYL
                     LQIRRYSPRNNERRNTVNEITGN"
     gene            complement(14341..14598)
                     /locus_tag="PKN31_03155"
     CDS             complement(14341..14598)
                     /locus_tag="PKN31_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOA98961.1"
                     /translation="MIDHVCLIVNNLEESRKYYERILNMESRESNTDSKTILLENKEV
                     HFFIIERDFPREYIEMQNAAYINSNRINRDKEAGIGGKRDN"
BASE COUNT         4608 a         3356 c         2748 g         4050 t
ORIGIN      
        1 tgtcatgtta gcaggaatat ctcttgtttc caccttattt acttttcaag gtacggataa
       61 tgaagcaaag agtattataa taggttagag cgtttttggc aagaggtaaa actgaattct
      121 gtagttattc tgtgataatg tcacccttaa aattctttag aaaatgtccc tttagtaata
      181 aaatgaaagg atgatacact atcctcaaga agaaagaaat aaactttatg gggcacgttg
      241 tcttctggat aagaagataa ctatcagaga gggagcagaa ctcttcacct atagcgtagg
      301 agcttgagtg gttaaagagc caaagttact caatttggca gacacaattt cgctcaggta
      361 ttaatctcat ctgagattta ttaatataaa caaccggaaa ccgcatgtag ctatttaatt
      421 aattggttac cccaacatta aagatccccc tgaaaatgcc gatatataaa atagagctaa
      481 aaaagcacac cggctccaaa gacccaaaga ccaaagagag gagaagagaa aatgaccttg
      541 aagactaaga ttttgagtgg tttcataata atgatagttt tagtagcaat tgtcggatgt
      601 attggttttt tcgccacctc caggttgaac gactctctat ataaagtata tgccgtgcga
      661 atgcccgctc ttgattacct tatcgaggca gacagggatt tacaacagct tctggtagct
      721 gaacgctcca tgctttctgc tcccagcgat agcgaaatat tcaaacgcct tcttgccgat
      781 tatgaagaaa attttaatca atcaatagag cgttttaaca agtataaaga actggctcaa
      841 gccccggaag aattgcaact tatatctgga tacgaagaag tccgtaaaga atgggaaaca
      901 gtgtcgaaac aggtaattaa ttatctcacc gaaggtggtg aggaagagcg taatctggca
      961 atagaactat ccctgggaaa agctgctgag aaattcgagg aaacacgcga atatttgaat
     1021 caactcaccg atgtcatatt gaacttaacc gaagaggaaa atcttagcgc tcagaaagcc
     1081 cgcaccatgt cactatcgct gataacctgg accatcatta taagcattgt tttaggatta
     1141 acattcgctc tactactcac aagaagcatc cgcaagcccc tccaggaaat ctccagtgca
     1201 gcagaaaagg tcgccagtgg caacttgcag gtcgaggtca aaaaactaac cgtaaaagat
     1261 gagctgggta aactcagcaa tagtttcgca gaaatggtaa attctctgag aaacctggta
     1321 cataatactt taaactcttc ttctcttctg gcatcttctt ctgaagagct tgcatcatcg
     1381 gtatccgaag ccagtagagc tactgcagag attgcccaaa ctatttccca ggtcgccgaa
     1441 ggctccattc gccagagtga agaactccag aaaatcgctg aagaaggaga taccatggct
     1501 aaaaaagctc agattatcgt caacacaagt gacgataatc tacgtcttct cgaggaaacc
     1561 atcaaaaatt tggaagaaaa tagccatatt ctctctacca ttagtaaaat gaataattcc
     1621 gttagcgacc aaagcaaaat cactcaagaa aaagctcaga atggcaaaaa attacttatt
     1681 gaccttgtag ccagcgtaga agatattgcc gaagtatctc aagaagttgc tcaaagtgtg
     1741 gagcttttga ctgaacgttc ccaggaaatt ggaaagattg tagacctaat aaccggtatc
     1801 gctgaacaaa ctaacctctt agccttaaat gcagcaattg aagcagcccg agcaggagat
     1861 gccggacgcg gatttgccgt ggttgctgaa gaggttagaa agcttgccga agagtctgct
     1921 caggctgcag aacaaattgg agctctgata tctgaaatcc agaaaaacac cggcgaaact
     1981 tctgaagcaa tgcagagagc aaaaaatcaa gtggaaacag gtagcaaaca gagcgaaaag
     2041 gtttctcaaa gctttgaaga tataatagca gcaaccggtg aagtcgccaa ctccattggc
     2101 agcttgacca gaactattgg agagctggaa acaaaccagc aagcaaccgc cgaaaattct
     2161 cagagtttag taaagcatac cgaagatacc ctgcacgatt tacgaagcat atctgagtct
     2221 attagcaaat tacatgagac catcactacc gttgcctcca ttgccgaaga gaacgccgcc
     2281 tccagtgaag aaatttccgc ttcagtagaa gaacaaaatg cagccatgga agaaattaac
     2341 tctatggcag ccagcttgag taaattagct gaagagcttc aagaagtcgt taacaacttt
     2401 actgtataag taatacatag ccaatcatat aggccacccc gctcaaaatt caaaagcgat
     2461 ggcggggtgg ctgttttttc attgagactg aatccctgct ccaggtaaat attgtcagaa
     2521 ctataaaagg gagcagcact cgcgattgac ccgtaataat gccccgacac aaaagataat
     2581 cagagtacga aggaaaagaa tattacaatg gatttttttg gttaactata tttttctggc
     2641 aggttctata gaagcaaggg aacaatactc acccctgtac tcagaggcgg caaattgatt
     2701 atagctcagt gatataatca atttgccgtg ctttttctga ggcggttaaa ataaattaga
     2761 atatttttgc tgtgaatgag gttgtagaaa tacagaataa tgtaagggaa aagaggtgaa
     2821 aatgtggcta tttaccatat tggtacttgc tcctggacag agaaaactct cattgaagat
     2881 ggctcttttt accctcctca acttaaaaat cctgctgaaa gactgaattt ctatgctcaa
     2941 cattttaaca ccgtagaagt tgattccacc ttttatgctt tccctgcaga gagaaatgct
     3001 cgtctctggg tagagcgaac cccttcctct ttccttttta atataaaatc cttttctcta
     3061 tttacctttc acaaaacttc cctatctgtc attcctcaat ttttgaaaga agaattgccc
     3121 gaaaggttgc aaaaacgacc tcacttctat ttagcggaag tccctgaaga gtggctaaaa
     3181 atagctctat ctgctttcct ttctgccata aaccccttgc aggaagaaaa taaactgggc
     3241 tatattctct ttcaatttcc tccctggata gaaaaaagta gagaaactat ggagtattta
     3301 tcctggctta gagaaaatat taaaggaact ttagctatag agtttcgcca ccattcctgg
     3361 ttgagcgaag aaaatcgaga tgacactttc tgttttttgc gcaaggagag attagcctat
     3421 acctgcgtag atgagcctca acttccctgg acggtccctc ccatagtaga agttactacc
     3481 ccggagctca taactcgctt ccatggacgc aataaaccag cctgggaaaa gaagggagca
     3541 cccgtagaag aaaaatttgc ctacctttat caaaaagagg aattaaacag gtggaaagag
     3601 gttgtaaaga agaaatcccc gggtgctgaa agagtttttt tgatgtttaa taactgtttt
     3661 cgtgattatg caataaggaa tgccaaacaa atgaaactac ttttggcgga atgaggtgaa
     3721 attatggaaa tcatcgatat atctcttcct ctgtcggaag aaactcccac ttataaaggg
     3781 agaaaggaaa aaaaacctcg tttagaaagg ataagaactc tttctcaagg agcaaacgaa
     3841 actcgcttga ccctggaaac ccacaccgga actcacgtag atgctcccct tcatatgctc
     3901 tctcccgcta actctattga tcagttgccc ctttcttcat ttcttggtcc ggctttagtt
     3961 gtagaaataa gaggagtgga ggccatagcc gcagaacacc tctctccttt tcaacaattc
     4021 ctatcccccc ggcaattttt aattctgaaa actgataact ctttttctga cctgtcgaga
     4081 gaaaactttg tttaccttac tgtatctggt gcttcttatt tagctcaaca aggtgcaaaa
     4141 ggcgtaggaa ttgactcttt agggatagaa cgcgaccagc ccggacaccc cacccatcgc
     4201 cttcttttag aaaaaggtat tttaatattc gaggggctat atctccggga ggtagaacct
     4261 ggcttttact attttctatc tttccctcta aaaataaaag agggagatgg ctccccgaca
     4321 agggctgtgc tctttagaat gtaaaatgaa aactttaata ttctttggag ctacgggcaa
     4381 tctcttcacc cagaaaatct tccctgcctt atcctctctt ttctatcact atgaaataag
     4441 agatttagct ttaatagctt taggaaggcg tttttctaaa gagggagact accggaggtt
     4501 tcttcaaaaa atccaccaga gagaagaaaa agagttattg gaacgggttt tctatctccc
     4561 aggagattta gaaaacccgg aaatcccttc aaaactcagc cagctaatta acaagacagg
     4621 gaaagaagag gcaatatttt acctctccct ccctccttct ctttatagct cagccttaaa
     4681 aaccatagaa aaaaccctgt ccttaagctc gccctccaca aaaaaagtag ctttagaaaa
     4741 accttttggt tccagcgaaa aagattttga agaactggaa aaagctctta aaaaaatctt
     4801 tcgagaagaa gaaattttct acgtcgacca ctacttggga aaagccaccg ttcaaaatct
     4861 catcattctt aaagctgaaa atctatttat agaaaaactg ctatcccggt ccttcgtaaa
     4921 agaggtgcgc attgcagtaa gtgaaaaaga aggggtagga gaaagaaaaa agttttacga
     4981 agagaccggt gccattaaag acgtatttca aaatcatctc cttcagcttc tcactctttt
     5041 agctatcgat atccctcccc tttgcgaaga agatagaaaa gagtgtcaaa gattcctgga
     5101 aaatgtaggc caggccaaaa gtcatttgct taccgagatt caactgcccc ctccggaagc
     5161 catatattta gggcaatacc agaattacca ggaagaggta gaaaatcctc attcccggat
     5221 agaaacttta gtttatctgc ctttatatat ttcttctgag cggtggcagg gagtaccatt
     5281 tcgaatgttg accggaaaaa gaatggcaga gagggaatcg tttcttgagg tggtatttca
     5341 ttcccatttt ccagaggata acctccttcg catagaaatt caacccgagg aaagaataga
     5401 cctcttaata agagtaaaaa caccgggaat ggggttatat tcttctccta cccggctaaa
     5461 tttcacctat tctggaagtt ttggagccaa tacccccgaa gcttatgaaa agataatata
     5521 tgacttgctg gccggggatc gaactttgtt tcctgactct acttttattc gtcatgcctg
     5581 gcaaatagta gaccggttgg tttctattat ccacaaggaa agaattaaac cccatcttta
     5641 ccccgtagac actttaactc ccggagattt ctggagctca cctccacaga ttacaatatc
     5701 ccgcaaaacc agtcatgatt ataggggata aacccccata acccctttat ccgtcatttc
     5761 tgaatggctt tatcaagggt ccagagagta gggagaatgc acccttatga ttccaaataa
     5821 aaagacaaaa gaaaaagcaa aaacgtggat gcctaataga agcattcagg catgacgaaa
     5881 gaggagaatt cccggctact aacctcggaa atggcgtggg agagtgggtt atcgatgaaa
     5941 gatttcagaa atgacagaag aagagccctt ccaggaagaa gctcaaggca agagtggctt
     6001 ctcttcctct gagaaaatga gaaaagcaag ttatcataga acaacctaag gtgtgaaatt
     6061 aaaagataga agataagaaa aaactaaagc ttaacgctgg aacatccttt ctccagcgtt
     6121 aagagagaaa ttgtatctcc ctcggtacac agttgtttct cttttttgag tctcaacaaa
     6181 tttaaaaacg aaattatcac gggaaataaa gatgcaaatc ccaaaaggat tatgatacca
     6241 tcaggatgct atgcaaaact tgcgattagc acccgggaca aagaaaacga agtaccgagc
     6301 aaagaagcgt caccatggat ttcttggtta gctacaacct aagtctctcg ggcaaaaatc
     6361 aaccgattag tcaatataac agagacaaga atgatacccc acagagcact ggctaaatga
     6421 gaactaagcc ctaaaaggtt aaaaccggta gctactacct gtaaaaccac catagataga
     6481 attaaaccac ctacctttcc aaatcctcca gcggggttta ctcctcctaa cacacaggcc
     6541 aatacagtga gcagaaggta tgattctcca tagcctgcct gagcagcatt gaaccgagca
     6601 gtcatgacta ctgaggctac cccagctaaa aaactacaga ggagatacac ccggaaaata
     6661 accctcctat tattgacccc ggaaaaaaga gtagcggtag gattagaacc taaaagatat
     6721 aattgaatac caaaaggagt tcgatccaaa agaagagaca taaatgcagc tgtaatcaaa
     6781 aagatgataa aaggcatggg aatacctaaa accgtgccat ctcctaggaa aagaaaactc
     6841 cggggaaaac cagctattac gtatccttta gttacaacca gagctattcc tctgataaaa
     6901 atcatcatac ccagagtagc aatcatagcc ggtatagagc aaaaagccac cagcgtccct
     6961 tttagaactc ctaaccccag agagagaagc aagcctaaaa caatggccca aaaaggtgaa
     7021 agttcaaact gagtcataga taaggcaatt acaatcgctg aaaggttagc ggtggaaact
     7081 atcgataggt caatcccccc ggttaacatg ggagacatct gagctaaagt taaaagaccc
     7141 agtaaaggca attgaaaagc gatagcctgc aagctccgag gttcaaaaat cttaccttta
     7201 gtaagcaagc caaatagcaa aaatatagct attaatatga taaccagaat aagaagctgg
     7261 ggatttctgc gctttaaaaa gggattactg gacaagaatt tcaccctctc tttttgcctg
     7321 agattttcgc tgataagcgc taatacctgc tgcgactaca attatggctc ccattatgac
     7381 ttcatgccag taagctgaag ctttcattaa aatcaagcca tttttaatga tggcaatcaa
     7441 taaaactccc aaaaaagccc ctaaagcact acccgagccc ccaaaaacgc tagctcctcc
     7501 taggataaca gcagctaaaa catccagttc tcctcccacc aaagaattgg gttgaatcat
     7561 ctggtttaaa gaagcgtgaa caaaagaagc cagaccggcc agagctccca taaaacaata
     7621 aacaaataat tgaacccgaa agatgttaaa ccctgctctc cgagcagctt ccatattgcc
     7681 acccatggca tagattttcc gcccaatagg gaagtatttg agtataaccc aggtaaagat
     7741 agccaaaatt atccatataa ggataaaaat cgagaaacca tatttcactc cctggggatt
     7801 agtcaaagaa aaaagctgca cccgggagaa atttcggaaa gaaggaggaa aattgtatat
     7861 ccattttcct ccactaaaaa acatcaaaaa accgaagaaa gcattgagag tggatatggt
     7921 gattatcacc ggatgagctt tagtgtaata gactaaaata ccgtttatag cccccagagc
     7981 aatcccgata ggtataggaa ttaaaaatgc tacgaggaca ttatctatat aataagtagt
     8041 tattatcaaa cccataatgt actgagcaat agtagcagta gcggtgaaag agacatctat
     8101 ccctccagaa attaaaacca ccagttcccc cagagccagg atgcctaaaa aagtattact
     8161 tcgtaacata tcaaaccagt tctccagagt aagaaacttg gggttcacca gagtaagaaa
     8221 aaaggaaaga acaatgataa ttcccagaag gtaaaattca gtctgctgaa agatcttttt
     8281 attaatcaaa ttatatcttt cctccctttt attccagaat gatttttctg agttcctctt
     8341 ctgatacctc agcaggagaa aattccctga ttatctggcc atttttcata actaaaattc
     8401 ggtcacagtt attcaaaact tcggtttcct catcagatat aaatatcaga ctgagatttt
     8461 tctgggataa ttctcgaata gactgataaa tgctttcttt agctgccaca tctaccccat
     8521 gagtgggggt atcgagaata agcagatgcg gagatgttaa aatccactta gctaacacca
     8581 ctttttgctg gttacctcca gagagatttt tcaccggtgc ttccatactt tcggtcttaa
     8641 tattaaattc ctgaacggct ttttctgcca cacttctttt cttctggaaa tcgatcaacc
     8701 ccatcccgtt gattatttgg tctagcacag tgattactaa attattctct acactgtggt
     8761 ccagcactaa cccttgaact aatctatttt ctggaacata gccaattccc aagccaatag
     8821 ctacctggtt ggaacttata acttgaggtt taccttcaaa atatattttc ccttcatcag
     8881 ggggatttaa tccaaagaga gtaagagcaa gctccgtgcg accagacccc cgaggaccga
     8941 ttaatcctaa aatttcaccc tggtagagag aaaaacttat cttttcataa tggccttttt
     9001 tagtaagatt ttctactctt agaatttctt cctctctcgg ataggctcca gcctcaacag
     9061 ggcgagaaga gatttcttta ccggtcatga gaaaagaaag ccgcttttta tccacttctt
     9121 ctcgctggaa agtccccact tttttcccat cccggagaac agttatccgg tcactgattt
     9181 ccagcacctc atccagccga tggctaatga acaaaatgga aatattccga gctttaagct
     9241 ctcgaataaa ggcaaacaat cggattactt ctttacgagt gagagaagaa gtgggctcat
     9301 caaggatgat aaatcgagca tcaacagcaa ttgctcgtga gatagctacc aattgttggt
     9361 cagcaaccga gaggcggctt acttcaattt ccgggtcaat atctaccccc aatttatcca
     9421 tcactcgggt agcttctgct ttgatttctt tccagtttac cagcttttgg gctttctcta
     9481 aataacggtg agaaaatata ttctctgcta ctgataaatt gggaaacaag gacaaatctt
     9541 ggtgaaccac gtaaatcccc aaacgaaagg catcaaaagg attggctatc tctaccttct
     9601 ggccgctaat ttcaaccttt gctccctttt ctggcctgac caccccggtt ataattttca
     9661 ccagagtgct ttttcccgaa ccattttctc ccaccagacc atgaacttca ccaggtaaaa
     9721 tagtaagatc cactccatct aaggccacta ctccaccata tcttttgctc gcattctgga
     9781 gaactaaata gggctttttt tgatcttcct cgattttctg agacaatgct tcaactctcc
     9841 tccgaatatt taaaaaagaa gaggggggga catccccccc ctctttagaa gcctaaagcc
     9901 tctgcttctt cggcagttct cattaccttg atctggttaa ccgagattac tttcccatca
     9961 atgcttatag gacccatgcc tggtatttcc attccttcga ctatttcttc tccatcaagc
    10021 atttgtttag caatatacac catggcataa ccagcgtccc tgggatccca aagttgagcc
    10081 caatctaccg ccccggtacg tagataagga gccacggtgg aaggaatagc actccccact
    10141 acaatgattt ggtcttccat tcccttctcc gtaaccgctt gcgctgctcc aacaggtcct
    10201 aaacttcccc aaccaatgac tgccttaagc tggtctccat aagtagtgat aaggtctaat
    10261 acggctgccc gggagtcttc cacgctttca gcagtgggaa gtctggaagt tagttctttc
    10321 aggaagggat aattttcagc agcatgctcc agagcagtat cagcccagaa gttgtggaga
    10381 ggaacagtca aacttcctac cagcatgaca aaaccagctg gattttcagg ggtgtatttc
    10441 tccagctctc ctgtttcttt taatttttcg actatagcat caacaggagc ttttccatat
    10501 tgcacactat caatggtctc tacatcccaa tctacagctt cagtctcaaa aggcgattca
    10561 tgagtgagaa tcactatccc ttgctctcta gcccggttaa acactggagc caaggatttg
    10621 gcatcactgg gaaccacaca tatcgcatct actccccggg taaccaaatc ttctaccatc
    10681 tggacttgag gagcagggtc agcagtagtt ggcccaacca tataagcatt taccccaaaa
    10741 tctttggctg cctgtttcac tccttcttcc aagcgactga accagggaat tccagcaacc
    10801 ttaaccacca ctgcaatttc atgctgctct tctgctccaa ctccaagagc aaaagaaaaa
    10861 actatagcca atactaacaa aaaactaatt aaagcctttt tcatacccaa tcacctccta
    10921 ttgatttttt gtattactta ttgcaaacaa tttaattgta aaaacatctc tctttgaaaa
    10981 tcatttaaga cacaattaaa gtatattcat tacctctgga gctgtcaata agaaaatttg
    11041 acctttgttc actatttcaa aattgaaatc ctccaccttt agcaagtaag tgactaaata
    11101 acatagaaca acaaagctat aaaggagtgt ctcttcataa ctcttagaag aaaaaaacat
    11161 taaaaagcaa ctacacagcc tattctttag atgatacact taaccgcaaa aacctttgta
    11221 acacccttct actcagcatt tcctcttctt gtctgtacct ttatattatt atttaactgt
    11281 cgtttgacta aacaatccag tatgctctta ccctattttt tagtcccttt aagatatttc
    11341 tcctctaaaa ctactgcaat cattttacac caatatgatc tcattcttcc tggcaaatcc
    11401 ggatagtaac tatacagacg atatccagat tcataattaa ccccccggga attcacaata
    11461 tggggagatg gtaaaaatat ttgtgggctg cgggtgatga attctcttta cgtttctggt
    11521 ttaagcacag ccgatgcttt aattttgctt tctttcaaca actgtaaaac acggttggcc
    11581 tctgtgagcg gaaaaatctc ggtctttgcc tcaagatgat gtttttgagc aaaatccagg
    11641 agttctcgaa catcctggcg ggtactattg gccacacttt ttatactccg ctcccaataa
    11701 aggagagaat agggaatctc cggtaggggg gtagaataaa ttccggccat aatcaccgct
    11761 ccacctcgct ccagataaag gagggcgttt ttaactaagt taccagcggg ggcaaatatt
    11821 atggcagaag tcagaagatg aggagggtgg tcttctgccc ttcctgcaaa agaagccccc
    11881 agatctcgag ctaaaagttg atgttcaggg cttctggtaa aaacaaatat ttccactccc
    11941 tctatttttg ctatttgagc tacgagatga gcagaggagc caaatccaaa aatacccaga
    12001 gtatctcctt tttttacccc gctcatccga taagctcgat aaccgattac tcccccacaa
    12061 agaaggggag ccaattcttc atctttatac ccttctggca atttatacac tgcctcttga
    12121 tgtgtggcta cgtattcagc atatcctcct agaacatcat agccagtaaa aagagcactc
    12181 tcacacaaat tttctcttcc gtcctggcag aagcggcacc tcccgcacac ccgatttaac
    12241 cagggaaccc ccacgagctc ccccggagac aggtctttta ccttttctcc cacctcttct
    12301 accactccca ctatctggtg accaggaatg agaggcaaaa ggtgatgcgg aatctcctcc
    12361 tctacaatat ggaggtcagt tcgacaaacc ccacaggctc taatccggat caaaacttct
    12421 tcctcactga tttgtggaag aggaaggtcg caaagtaaaa gaggatcagt ctctatagga
    12481 gcgggttttt ccagcaccaa agctctcatt gttttgcctc cttaaaccat tcccaggtgt
    12541 ggccaatccc ttcttctaag gaaaaagaag gagtccagcc cagagcttct ctggcttttt
    12601 ccgaagagag agcaatacgg tctatttctc ccttcctctc ttctccgaac actaccggga
    12661 aatcttctcc gctaactttt ttcaaaacct ccaccaggtc taaaacactg gtttctttac
    12721 ccgttcctaa attatatatt cccgaggcag atagagctaa aagattagct ctggctatat
    12781 cttctatata aacgtagtct cgagtttttc gcccatcacc aaagatgata cagggctcac
    12841 ctttaattat ccgagagata aagatggcta caactcctgc ttctcctcga gggtcctggc
    12901 gagggccata aacgttgcca tagcgcaggg ccacaaaatc cagaccttgc actttatgat
    12961 aatagttaag atatagctct accgaacgct tagccactcc ataaggagaa agaggtaaag
    13021 caggcatgtc ttcacgagcg ggaagtgttt ttacctctcc gtagatagct cccccggtgg
    13081 aggcaaaaat aaatttttta actcggtatt ttacgcttag ctctaaaaga gaaatactcc
    13141 ccaggatatt tacccgagca tcaaaaaggg gctcttctgt ggagcgacgc aaattgatct
    13201 gggcagcgtg atgattgaca taatctggct tttcccgaaa aaatatttcc tccatatccg
    13261 acagagaagt tatatctcct tcataaaaag tcactccccg gggtagattt tctcttttgc
    13321 cggtagaaag attatccacc accactacat caaagcccga agcaagatag gcttccgcta
    13381 tatgagagcc gataaaaccc gctcctccgc ttataagtac tttagccact ttattcctcc
    13441 ttctctcctt actacctaat ataaaagcac aaaacctctc tcaaataaac cctttctgaa
    13501 gtaaatcttg ctctttcctc aagaatcaca ggaaaatccc tgaaggggag attctgcgga
    13561 agaacagctt caagctcctc attaatttcc cgttatttca ttcaccgtgt tccttctttc
    13621 gttgtttctg ggagagtatc ttctaatctg cagtagataa tcccccgggt actctttata
    13681 gttagttttt ccataacttt tagtattcct tttaaagaaa gctcccagtt cagcgaggaa
    13741 tggttcttcc gcccctctca cctgtggaat actttctgga ttcataccca ttacgatgtc
    13801 ctgatttatt ttcctcttct tttgctcttt ataatgattt atttgggaat tcaattactt
    13861 ctgcttccaa tagttgctgg aaaaccaagc tccccttttt tcatgactat attcaaccag
    13921 ggctctctat cttctaattt tcttcaggct aatcaatcaa aaattccttc taatatttga
    13981 tgcaactcct ttctcgcgct cactctttgg tattcttttc ataagtagat ggtctccttt
    14041 ctatgaaatt ttccacgaga cttactttgc cacaaaggcc atccactttt ttgttttatg
    14101 aggatacaga acataaccaa gctgccctgc tggtagcata agggatgagt tcgatgagag
    14161 ccgctcgaat attttcagct aaaaacttga ccagctctga ctcagattcg agaggttgtt
    14221 atgttgagtt cagttcctta aattgattca acatattcaa gccgtattcc atcaggatct
    14281 cgccattcaa tccatttata attgtgatat cgaaattgtc tgaattctcc cattgttaat
    14341 tcagttatcc ctttttccac caattcctgc ttctttgtct cgattgattc tgtttgaatt
    14401 gatatatgct gcattttgca tttctatata ttctctcgga aaatctcgct ctattatgaa
    14461 aaaatgcact tctttgtttt caagcaatat tgttttacta tccgtatttg attccctgct
    14521 ttccatattc aggattcttt catagtactt ccgtgactct tcaagattat tcacaatcag
    14581 gcatacatga tcgatcattt ctattcctcc ttatattcca gttctattga tctctcataa
    14641 aatcccactc tatgatagaa atcaagtact ggaaacggtg actacaccca acttgcgcta
    14701 taagtgtcag aaattagcgc tataacaggc ttttcttttt aaggactccc attttctggt
    14761 aa
//
LOCUS       DAOPSS010000030        14542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_3522_length_14542_cov_8.640298, whole genome shotgun sequence.
ACCESSION   DAOPSS010000030 DAOPSS010000000
VERSION     DAOPSS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 14542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14542
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3522_length_14542_cov_8.640298"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..348
                     /locus_tag="PKN31_03160"
     CDS             61..348
                     /locus_tag="PKN31_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOA98962.1"
                     /translation="MAEISELSSGRKEITIEENKILLLNLNGEIYAIGSRCPHKGLSL
                     AQGKIEGNIITCPYHGSRFDVVTGQVIKGPAKEGLPVYEVKVEEKDILISL"
     gene            360..824
                     /locus_tag="PKN31_03165"
     CDS             360..824
                     /locus_tag="PKN31_03165"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOA98963.1"
                     /translation="MKKIGQVLKSEEKYSWVKMEKGTSCGEEKCPLSSPLIDDSKSDF
                     YVVKAYNDLGALPGDKVLVEINDHIALGVAFLIYIFPILLTLSVYFLLRYLTPLPLYH
                     YSGVAASIIFSFFILKKGDQKLQLNYRITDFADSICERCPFSSGTTVSSLPE"
     gene            complement(887..1687)
                     /locus_tag="PKN31_03170"
     CDS             complement(887..1687)
                     /locus_tag="PKN31_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA98964.1"
                     /translation="MQYIDLYGARVPVIGLGTWDLRGETCERAVLSALNLGYRHIDTA
                     QSYANEEEIGRALQNSSVPREEIFLTTKVWYTHLRYSEVIQTLEESLRKLKTDYVDLY
                     LIHWPSEDVPLEETMSAMIEVKEAGKTRYIGVSNFDIPLLEKARQISPYPVLTDQVEF
                     HPYYSQKELLSYCQKNKVILTAYSPLARRRLSGDLLLQEIGKKYGKTANQVALRWLIQ
                     QEMVAAIPKAANPEHQKENLDIFDFELTPEEMEKISRLDRREKVASRT"
     gene            complement(2254..3171)
                     /locus_tag="PKN31_03175"
     CDS             complement(2254..3171)
                     /locus_tag="PKN31_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOA98965.1"
                     /translation="MPNQKRKWKVSYLGKWLLERVVVPSLLKILEGLVLILPPAALKR
                     FAFSLGDLIYHLPFFDPQLAKTQWMNIMGEKDGKVVNRHIQQVFHHFALSFMEVVLLK
                     KKGERVLEDLVEKVEGEEFLKKALSQGRGVILLTAHLGNWEVLGAWLGSQGYPTATLV
                     RSPSVPAIRRFMEELRSVMKLEMVERTNLLPVRSLLQEGKILGVLADQYALKGVACDF
                     LGQKTLSPATPAVFYLRLGSPIIPAFIVRQGEEMKHQITFLPPLDWPLTASYQENIVR
                     GTALCNQILGEFILRYPEQWNWFHRRWRD"
     gene            complement(3164..4486)
                     /locus_tag="PKN31_03180"
     CDS             complement(3164..4486)
                     /locus_tag="PKN31_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOA98966.1"
                     /translation="MSSDQEQLKLPWFYQVLHNFYIFLLGLIAAVFYPYISRFYAFSP
                     ASLYSSSKIKQSNQRPKIWIHGASAGEIGAALSLCSCLRERYPHSYIVCSAANPDGKR
                     MAQEILAPLVNEFISYPLDLSWVVSRFLDFIQPDIFITMEAELWPNFLFSAKRKGIKC
                     YLVSGRVSQQTFYWTQKIKNYWRNVLLSFDTLFMADQLSYERVLKFGLPEDKVLLLGN
                     IKTDALYERKKKSQPERLAQNLGLAADLPVIVAGSIHKEEEELMLEAFDKVRKMVPQV
                     KIIIAPRHLDCCSQLVQLFKEKNFAVTSLSKLPADEEWEVLIIDQMGVLFDFYGLAQV
                     AFVGGSLCKNMRGHNILEPAIWEIPVCYGPYVASFQEVASVLKSKGLSRVVHNSEELA
                     YAWYQGITDTEWRKNAQMEAHHYFAQAGRITEKVVDIITKDWEQSRNA"
     gene            complement(4546..5082)
                     /locus_tag="PKN31_03185"
     CDS             complement(4546..5082)
                     /locus_tag="PKN31_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOA98967.1"
                     /translation="MYKVIVATDYPDILQAVESFGGVAQMTSSTCECGTDRVAEVAKM
                     YPEYDLVINVQEDQVEFSPSALDDLVLSLEHDSLASMATPIQLLQDEEAFNNPNVVKV
                     VCDQDLNALYFSRSHIPYPRSSWGSWFKHIGIYAFWRDFLFQFASWSPTTLEKSESLE
                     QLQALKQGKRIKCVFDDG"
     gene            complement(5406..6260)
                     /locus_tag="PKN31_03190"
     CDS             complement(5406..6260)
                     /locus_tag="PKN31_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOA98968.1"
                     /translation="MTKLVNRVVFWIIVGLIMLYLLFPFYWAVNSSLKNENQLMMTPT
                     TFIPRDPVTKEFSPTLDNYRAVFQSDQFIRGLRNSAIVAGATTLLSLAIGSFAAFALG
                     KLRFRGKSSALYIVLSMTMFPQVTILPGLYAVITGLGLTAIPGMILSYLIFTLPFTVW
                     VLTSFFRELPIELMQAAQVDGATPFQAFRLILLPLTAPALVTAGLLAFIAAWNEYLFA
                     LTFTAIEPASRTVPVAIALFPALVARQEPFGEIMAAAVVVTIPIVVLVLIFQRRIIAG
                     LTAGAIKG"
     gene            complement(6269..7342)
                     /locus_tag="PKN31_03195"
     CDS             complement(6269..7342)
                     /locus_tag="PKN31_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOA98969.1"
                     /translation="MFKKNASSKLLQREQRLAYLLLLPTFVVLIVVALYPLGLVFVTS
                     FTDRTFASPREPNYVGLNNYTQLLGMTIRELPPLIDEETGLQVVDPKTGGPLYERPLR
                     VLPREPRRYREVTQFSLFGKRYVLGATDPDFIKAVRDTLVFTVVSVLLETILGLGIAL
                     VVNSPFKGRGVMRAAMLVPWAVITVVSARMWQWMLAPTRVGVINVIFQMLGLGDGNIA
                     FLTQPEWQLPSIIMVDVWKTTPFMALLLLAGLQLIPDELYEAARVDGAGPMRQFISIT
                     MPLLRPTMAVALVFRTLDALRVFDLFQVMLAQSRYSMASYNYYQLIGNRAMGKASAIG
                     VIMFIFIFIFAVLYIRMLGVETD"
     gene            complement(7520..8815)
                     /locus_tag="PKN31_03200"
     CDS             complement(7520..8815)
                     /locus_tag="PKN31_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA98970.1"
                     /translation="MRLNRYLVAVCNLIIAIFLIGGLAMAQEKVVITVAAGAVGQELE
                     LTREAAQRYMEAHPDVEVRVIDTPDLATDRYGLYLQFFEARSSDIDVYQIDVIWPGDL
                     AEHLLDLYEFGAKEIADEHFPAIIENNTVDGRLVGIPWFTDAGLLYYRTDLLEKYGLE
                     VPKTWAEFEEVARIIQKGERAEGNEDFWGFVWQGNAYEGLTCNALEWICSNGGGTIIS
                     PDGVITINNPNAIEAIEMAARWVGTISPAGVTGFAEEDARSLFQSGNAAFMRNWPYAY
                     ALGQSEESVIAGKFDVASLPAGRSGHGAATLGGWQLAVSKYSNNPEIAADVALFMASP
                     EEQKIRAIKGSFTPTIMTLYEDPEVLESAPFFGTLYDVLINAVARPSTVTAPNYNEVS
                     TLFFRAVHSVLTGAKDATTALEELELELQALTGFEIGEP"
     gene            complement(8953..9966)
                     /locus_tag="PKN31_03205"
     CDS             complement(8953..9966)
                     /locus_tag="PKN31_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA98971.1"
                     /translation="MSRKLLEVKSVVKIFHIGGRFLGTKLKAVNNVSFDIDSEKPEIL
                     TFAGETGSGKTTLARMILGLEQPTSGEITYKGKNVSHIQKREELLEYMKEVQPIFQNP
                     FETFNPLRKVDSYLFETAGNYNLAKTKGEKAKLVEEALKSVGLTLNEVQGRYPNELSG
                     GQLQRTSVARALITRPSLLIADEPVSMVDASLRMSIVNLFSDLKEEYNVTVLYITHDL
                     ATAYYISDRIAIMLRGDVVEFGNIDKVMLSPLHPYTQILLKSVPQPDPEGTWKEDIKL
                     SSLEVKEFSRLGCKFYERCPSAMKICEEVEPLSVFRDGREVKCHLYSEELKKEEETLP
                     GSF"
     gene            complement(9941..10966)
                     /locus_tag="PKN31_03210"
     CDS             complement(9941..10966)
                     /locus_tag="PKN31_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA98972.1"
                     /translation="MSLLEVENLSAYYITHAYGVQRDVRAVDNITFSMEENEIVGLAG
                     ESGCGKSTLIKVLLAIIKPPLRVLSGQVNYSYDGKKFNLLTLEREQLRKVQWEDISYI
                     PQGSMSTLNPVRKIRKTFEDVIKAHKKSESREEYERLMEEHIKYLGLPLEVLDSYPHQ
                     LSGGMRQRTTIALATILKPKIIFADEPTTALDVVVQRGVIQMLKQLHEEQKNTMLIVT
                     HDMAVHANVAEKIAIMYAGKIVEIAEKKELFTAPKHPYSQFLIQSLPRIGDKRRRTSA
                     PGNPPSLADPPPGCRFHPRCPYVKDVCKRHIPKLIEYEESHNIACFLYSSEIEEGEEI
                     VQEIIRS"
     gene            complement(10973..11836)
                     /locus_tag="PKN31_03215"
     CDS             complement(10973..11836)
                     /locus_tag="PKN31_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA98973.1"
                     /translation="MFVILKDLLKRDKKFLFGFIVVCILVFLAILSIFSPYDPRSWSV
                     VPKDRPPSFQYILGTNSMGQDIFWNTTHALKNSFVLGITTAVVATIIGTIVGLIAGYR
                     GGTLDKVLMSINDSFIVLPSLPILVFLSFSLRGKMTMFVLGLTLSVFSWPWGGKQVRA
                     QVLSLREREFTYTSVFSGMNMWKVVFREHLPFVIPWVIANFINTILWALGMEITLSVL
                     GLTSLETPTIGTTIHWAMQYQAIFRGIWWWLLTPIILCVLLFVALYMLSVSISEFLDP
                     RTRLQRIKMGG"
     gene            complement(11846..12856)
                     /locus_tag="PKN31_03220"
     CDS             complement(11846..12856)
                     /locus_tag="PKN31_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA98974.1"
                     /translation="MNALKTYIIPRLIQYVVVIFIGITITFIIPRLTPIDPVQTAINR
                     LTSYGGIYMEPEIVESLQNTLKELYGLEGSLLEQYFAFWKHLFTLDFGPSLSMFPTPV
                     IEIIANSLPWTVFLLLTTLLISWIIGNILGGLAGYFKDEKWSNVLAIIAMSIYPIPYY
                     VMALILVILFVYIFPVFPMMGGYSIGIQPSLSWAFIVDVLRHSILPAISLIIIGIGWW
                     FLSMRSMTSNVVAEDYVTYIEFAGLPRHKVLFSYVMRNAILPQVTALALQIGGIFNGA
                     VITEVAFSYPGVGLLLKNAITNGDFNLMMGIAVFSIIAIATASLVIDLLYPLFDPRIR
                     YR"
     gene            complement(13130..>14542)
                     /locus_tag="PKN31_03225"
     CDS             complement(13130..>14542)
                     /locus_tag="PKN31_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA98975.1"
                     /translation="ELTKSYSRMHNIFTSLVYGALFMQPKHIWEEIENPLTFDFNPPV
                     SLGPYVLDSYDSQGYWFLFKKRDDWERTSVGQLYGEPKPSYVLFIYYGTDDRKVMAQA
                     RHELDMIFDLTPEAWEVLRERNEHSVVWYRDFPWAWMDDVNSRGMFLNNARYPFDIKD
                     VRWALTLATNIEEIIISGYAGIERVSPLHSCPTQYFTEIYFKPLEPWLEDFTIDGDYK
                     PYDTEVAYRIAEWAEEQYNIEVEDPEAVWGIGWWKYDPQKAEDLLLKNGFSKDQNGKW
                     LLPDGTPWNISIIAPADFEIDATRLAFAVADQWRKFGVEVNVETLEAGPLWSRQAIGD
                     YDAGSFWGGMLASSGIPDIWTFINGFKSDFLEPLGTPVTTANNYRWKVPERVDEIIDE
                     MGALYPGDPKIIELAKEFYKLAVEEMPTIPMVINKKFNANDNYYWTNYPSADSPYMAP
                     VSWWAEFKFMFPRIEPTGRE"
BASE COUNT         4390 a         3326 c         2687 g         4139 t
ORIGIN      
        1 ttgctttgct acatttttac accattaaag aggagggaaa aacatggccg agttaaaaag
       61 gtggcggaaa tttcggaatt gtcatcaggt aggaaagaaa tcactataga agaaaacaaa
      121 atccttcttt taaatttgaa cggggaaatt tatgctatcg gtagtcgctg tccccacaaa
      181 ggtctttctc tggctcaggg gaaaatagaa ggaaatataa ttacctgtcc ctatcatggc
      241 tctcgctttg atgtagtcac tggtcaggtg ataaaaggac cagctaaaga gggcttgccg
      301 gtttatgagg tcaaggtgga agaaaaagat attttaatta gcctgtagta aaaactaaaa
      361 tgaaaaaaat cgggcaagta ttgaaaagcg aagaaaaata cagctgggta aaaatggaaa
      421 aaggaacctc ctgtggagag gagaagtgtc ccctttcctc tccactcatc gatgactcta
      481 agagtgattt ttatgtagtc aaagcttata atgacttagg tgccttgcca ggagataagg
      541 ttttagtgga aataaacgac cacatagcct tgggagtagc ttttttaatt tacatttttc
      601 ctattttact cactctctct gtttattttc ttcttcgcta tcttaccccc ttacctttat
      661 accattattc aggagtggct gccagtataa tcttttcctt tttcatactc aaaaaaggag
      721 atcaaaaact ccagcttaat tatcgaataa ccgattttgc tgattctatc tgcgagcgtt
      781 gtcctttttc ttctggtacc accgtgtctt ctttaccaga ataatagatg ccacctgacc
      841 ctccacagct atctaaaaat tcaaaaaagc gacaaactct ctccttttag gtgcgagaag
      901 ctactttttc ccgccggtcc aggcgagaaa tcttttccat ttcttcagga gtaagctcaa
      961 aatcaaaaat atccagattc tctttttggt gttcggggtt agcagcctta ggaatagctg
     1021 ctaccatttc ctgctgaatt aaccagcgca gggctacttg attagccgtt ttaccatatt
     1081 ttttgcctat ttcctgcaac aacaaatcgc cactcaacct tctccttgct aaaggagaat
     1141 aagcagtaag gatgaccttg tttttctgac aatagctcaa aagctctttt tgagaataat
     1201 agggatggaa ttccacctgg tcagtaagaa caggataagg agaaatctgt cgagcctttt
     1261 ctaaaagagg aatatcaaaa ttactgactc caatatatct ggtttttcct gcttccttta
     1321 cctcaatcat ggcgctcata gtttcttcta agggaacatc ttcgctgggc cagtgaataa
     1381 gatagaggtc tacatagtca gtttttaact ttcgtaaact ttcttccaga gtctggataa
     1441 cttcagaata acgcaagtga gtataccaaa ctttagtggt gaggaaaatt tcttcccggg
     1501 gaactgaaga attctgcaac gctcttccta tctcttcttc gttagcataa gactgagcag
     1561 tatcaatatg acgatatccc agattaagag cgcttaacac tgctctctcg caagtttctc
     1621 ctcgcaaatc ccaggttcct aaaccgataa ccggaactcg agctccatat aagtctatat
     1681 actgcattcc tctacctcct cattttttct cattttacca tgtaaatact actttcccat
     1741 catcctttta ctactccagc agttagtcct ttacaattcc tctttgaaaa atgattacca
     1801 ttgttatcaa cagcaaggcg gccaaagtgt aactgccaaa attcggtctc agggaattgc
     1861 atatcaggaa gctccgataa ctcaagtaaa actctaagat agaaatacaa tgtaaatatg
     1921 gatgcaattg cctcttcttg ctatctcact atatcacagc tactaatccc caagtcagca
     1981 atccaagtac catatttata aacttatacc cccaaatttt tcagaaagct tcttcttcca
     2041 ctattttaat ggcagagaaa acttcggttg cggttcttct caaaaatata atcctttagt
     2101 tatcaaagct tccagcataa aagcatatat tgctatttct ttcgttcata tatttccatt
     2161 acaatacctt aaacactggc ttaccattgg gaacggaaga gatattgaaa aacaggaaga
     2221 attgacaaat ttagcaagag tagagtgcat aagctaatcc cgccacctac ggtgaaacca
     2281 attccattgt tcaggatagc ggagaatgaa ctctcctaaa atctggttac agagagccgt
     2341 ccctcgaaca atattttctt gataagaagc agtgagaggc cagtctaagg ggggaagaaa
     2401 ggtaatttga tgtttcatct cttctccttg gcggacaata aaagccggaa taattggact
     2461 tcccaatcga agatagaaaa cagccggagt agcaggagag agcgtctttt ggcctaaaaa
     2521 atcgcaagct actcctttta gtgcgtattg atcggccaat actcccagta ttttgccctc
     2581 ttgcaggagt gagcgaactg gaagcaggtt ggttcgttct accatctcca atttcataac
     2641 ggatctcaac tcttccataa atcgccgaat agccggaacc gaaggggaac gaacaagagt
     2701 agcagtagga taaccttgac tacccaacca ggctcccaaa acctcccagt ttcctaaatg
     2761 agcagtcaac aaaattactc cccgaccttg agatagagct tttttcagaa attcctctcc
     2821 ttctactttt tccaccagat cctccagcac tcgctctcct ttctttttca atagtaccac
     2881 ttccataaaa gatagggcaa agtggtgaaa cacttgctgg atatggcgat taaccacttt
     2941 tccatctttc tctcccataa tattcatcca ctgggtttta gccagctgag gatcgaaaaa
     3001 aggtaaatga tagattaagt ctcccaagga aaaggcaaat cttttgaggg cggcaggagg
     3061 aagaattaaa actaaaccct ccaaaatttt caggagagaa ggaacaacca ctctttctaa
     3121 caaccatttt cccagataag aaactttcca ttttcttttt tggttaggca ttcctacttt
     3181 gttcccaatc tttggttatt atatctacta ccttttcagt tattcgacca gcttgagcaa
     3241 aatagtgatg agcttccatt tgggcgtttt ttctccattc ggtatcagta atcccctgat
     3301 accaggcata agccaattct tctgagttat gtactacccg agacagtccc ttagatttta
     3361 aaaccgaagc aacttcctga aatgatgcca cgtagggacc gtaacaaact ggtatttccc
     3421 agatagccgg ctctaagata ttgtgacctc gcatattttt acaaaggcta ccccccacaa
     3481 aggcaacttg agctaaacca taaaaatcaa aaagcacccc catctgatca attatcagca
     3541 cctcccactc ctcatctgct ggtaatttag aaagagaggt tactgcaaaa ttcttctctt
     3601 taaaaagctg aactaattgc gaacaacaat ctaagtggcg aggagctata ataattttaa
     3661 cttgaggtac catttttcgt accttatcaa atgcttccag catgagttct tcttcttctt
     3721 tatgaatgct ccctgctact atcaccggga gatctgctgc caaacccagg ttttgagcca
     3781 aacgctcagg ctgtgacttc ttcttccgct cataaagagc atcagtcttg atatttccca
     3841 aaagtaaaac tttgtcctcc ggtaaaccaa atttcaaaac ccgttcgtag cttaattgat
     3901 cagccataaa cagagtatca aagctaagta aaacgtttcg ccagtaattt ttgattttct
     3961 gtgtccaata gaaagtttgt tgagaaaccc ggccgcttac caaataacat tttatccctt
     4021 ttctttttgc tgaaaaaaga aagttgggcc aaagctccgc ttccatagtg ataaatatat
     4081 caggttgaat aaagtctaaa aagcgggaga ccacccagga aagatcaagc ggataggaaa
     4141 taaactcatt caccaaagga gcaagtattt cctgagccat tcttttccca tcagggttcg
     4201 cggctgaaca cactatataa gagtgagggt atcgctctcg cagacaagag caaagagaca
     4261 aggccgcccc aatttctcca gcactggctc catgaatcca gattttgggt cgctggttac
     4321 tttgtttaat cttactgctc gagtaaagag aggccggtga aaaagcataa aacctactaa
     4381 tataagggta gaaaacagcc gcaatcaacc ccaaaagaaa gatataaaag ttatgaagta
     4441 cttggtaaaa ccagggtaat tttaactgtt cctggtcaga actcattttc ttttttcttg
     4501 gccaaatatc tatcatagtc ctcgcggttg ctaatatccc agctttcacc cgtcatcaaa
     4561 aacacacttg attcttttcc cttgtttcag cgcttggagc tgttctaaag attcactttt
     4621 ctcaagtgta gtgggactcc aggaagcaaa ttgaaataaa aaatctctcc aaaaggcata
     4681 aatacctata tgcttaaacc aagaacccca acttgaacga ggataaggaa tatgggaacg
     4741 gctaaaatag agagcattca aatcctggtc acaaactacc ttaaccacat tagggttatt
     4801 aaaagcctcc tcatcctgga gcaattgaat aggggtagcc atggaagcca aagaatcatg
     4861 ctccaaagat agaactaagt catccaatgc tgaaggagaa aactccacct gatcttcctg
     4921 aacattgatt actaaatcat attccggata cattttagct acttcggcta ctcggtcagt
     4981 tccacattca caagtagatg aggtcatttg tgccactccc ccgaaagact ccactgcttg
     5041 aagaatatca ggatagtcgg tagccactat cactttatac acctaccgct tttttgaact
     5101 tgttgcatta ctttaaccac catcggaacc ccatctatag gtagtaaggt tttgcctgaa
     5161 aggcggctag attggtaacg agccgaaata accactatcg atttgaaaca atcaccttta
     5221 ttcaatgttt tttcgataga aatagaacaa atttttgcca atcccttgct tctcgtaaaa
     5281 taaaaccggc cttttacatc cacttgagaa aagtgcccag tttttattta atctctctga
     5341 aaacaatttg tcaagcatac gagggcttta taacaaattg tgtggaaaaa tatctagact
     5401 tctttctacc cttttatggc tcctgctgta agcccggcaa taattcgccg ctggaagatt
     5461 aagaccaaca caactatcgg tatggttacc accacagcag cagccatgat ttctccaaat
     5521 ggctcctgac gggcaaccaa cgctggaaag agagcaatag ccaccggaac agttctagaa
     5581 gccggttcaa ttgcggtaaa agttagagca aacaagtatt cattccaagc tgctataaaa
     5641 gccaaaagac cggctgttac cagagctggc gcggtcaggg gcaagagaat taatcggaaa
     5701 gcctgaaaag gggtcgcgcc atctacctga gctgcttgca tgagttctat aggaagctcc
     5761 cggaaaaaag aggtaagcac ccacacagta aaaggaagag taaaaatcag ataggataaa
     5821 atcatccccg gaatagcagt gaggcccaag ccggtaatta ctgcatatag cccaggtaaa
     5881 atggtaacct gaggaaacat ggtcatagaa agaactatat agagtgctga ggatttccct
     5941 ctaaagcgga gctttccgag agcaaaagca gcaaatgaac ctatggctaa agacaaaagg
     6001 gtagtagcac cggctacgat agcgctgtta cgcaaacccc ggataaactg gtcactttga
     6061 aacactgctc ggtaattatc cagagtggga gaaaactcct tcgttaccgg gtcacgggga
     6121 ataaatgtag ttggagtcat catcagttga ttctcgtttt tcaacgaaga attcactgcc
     6181 cagtaaaaag gaaacaatag gtacagcata ataagcccga cgattatcca aaaaacaact
     6241 cggttaacta atttagtcat agatttactc aatctgtctc cacccccagc atccggatgt
     6301 acagcacagc aaaaataaaa atgaagatga acataatcac cccaattgcc gaagctttac
     6361 ccatcgctcg gtttccaatt aactgatagt aattatagct ggccatcgaa tagcgactct
     6421 gagctaacat cacctggaat aaatcgaaca cccgtagagc gtcaagggta cggaacacca
     6481 gagctaccgc catagtgggc ctcaaaagcg gcatggtgat ggaaatgaac tgacgcatag
     6541 gacctgcccc atccactcgg gcagcttcat acagctcgtc aggaatcagc tgtaacccag
     6601 ctaaaagaag caaagccatg aagggagtgg ttttccatac gtccaccata atgatcgatg
     6661 gaagttgcca ttctggttga gtaagaaagg caatgttccc atctcctaat cctaacatct
     6721 gaaaaatcac attaattaca cctacccgag tgggagcaag catccactgc cacatccggg
     6781 ctgaaacaac ggtaatcacc gcccagggaa ccagcattgc tgcccgcatc acacctcgtc
     6841 ctttaaaagg actatttaca accagtgcaa tacccagtcc cagaatggtt tccagaagaa
     6901 ctgatactac agtgaagacc agagtatccc gtaccgcttt gataaaatca ggatcagtag
     6961 cccccagaac atagcgtttg ccaaagagcg aaaactgggt tacttcccgg tatcgacgtg
     7021 gctctcgggg aagaacgcgc agtggacgtt catacagtgg tcctccagtt ttcggatcta
     7081 caacctgaag accagtttct tcatcaatta aggggggaag ttcacggatg gtcataccca
     7141 gtagctgagt ataattattt aatcccacgt aatttggctc acgtgggcta gcaaaggtac
     7201 ggtcggtaaa actggtcaca aaaaccagcc ccaggggata tagagcaacc acgatcaata
     7261 ccacgaaagt gggtaacaat aaaaggtatg ccagtctttg ttcccgctgt agcaattttg
     7321 agctggcatt ttttttaaac atcatagctt tctccggtta ccagcaaggc ctttagcctg
     7381 gttttatgaa aaaccaaact gaccgcggaa aaaaagcaac cgaattccac ctcctctcct
     7441 tttatttccc ctacatcggt cagcgtaaaa tcagataaaa aagctccagc ccgttacaag
     7501 ggctggagct tttggtcttt tatggttctc cgatttcaaa accggtgagc gcttgaagtt
     7561 ccagttccag ttcttcaaga gcagtggttg catctttagc cccggtaaga accgagtgca
     7621 cggcccggaa gaagagagta gatacttcgt tgtagttggg agcagttact gtggagggac
     7681 gagccacagc attgattaat acatcgtaaa gagtgccaaa gaaaggagcg gattctagaa
     7741 cttcaggatc ttcgtaaagg gtcataatag taggagtaaa agaaccttta atagctcgaa
     7801 ttttctgttc ttcgggagaa gccataaaca gagccacatc agcagctatc tccggattgt
     7861 tgctatattt actcaccgct aactgccagc cgcctaatgt tgcggcacca tggccagatc
     7921 gcccagcagg tagagaagca acatcaaact taccggcaat tacactttct tcggattgcc
     7981 ctaaagcgta agcgtaaggc caattgcgca taaaagcagc gttaccagac tggaaaaggc
     8041 tccgggcatc ttcttcggca aaaccggtta ctcctgcagg actaatagtg cctacccagc
     8101 gtgcggccat ctcgatagct tctatggcat tggggttgtt aatggtaatt acgccatccg
     8161 gactgattat ggtgccacca ccattgctac aaatccattc cagagcatta caggtaagac
     8221 cttcataagc attaccctgc cagacaaacc cccagaaatc ttcattgcct tctgctcgtt
     8281 cacctttctg gataattcga gcaacttcct caaactcggc ccaggttttg ggcacttcta
     8341 atccgtactt ttccagaagg tcagttcgat aataaagaag gccagcatcg gtaaaccagg
     8401 gtattcccac cagccgaccg tcaacggtgt tattctcgat aatagccggg aagtgctcat
     8461 cggcaatttc ttttgctccg aattcgtaga gatctaagag atgctcagcc aggtctcccg
     8521 gccagattac atctatttga tagacatcaa tgtccgagct acgggcttcg aagaactgaa
     8581 gatacagtcc atagcggtca gttgccaaat caggagtgtc gattactcgc acttccacat
     8641 cggggtgggc ttccatatac cgttgagctg cttctctggt aagttccaat tcctggccta
     8701 ctgctccagc agcaacagta atcaccactt tttcctgagc catcgccaga ccaccaatta
     8761 aaaatatggc aataatcaaa ttgcataccg caacaaggta tctattcaat ctcattttta
     8821 ttaccccctc tttagaaatt aatcatacca attaccttat caccgattac tacctgaatt
     8881 accttatcag ttctttctct tttagccaca cctccccgca tcacctccta taacttaaaa
     8941 ctcaaatagg aatcaaaaac ttccaggtaa tgtttcttct tcctttttca attcctcaga
     9001 atatagatga cactttacct ctcttccgtc tctaaaaaca ctcagcggtt caacctcttc
     9061 gcagattttc atagccgaag ggcacctttc gtagaatttg cagcccaacc ttgaaaactc
     9121 ttttacttca agagaagata gttttatatc ttctttccac gttccctcgg gatctggttg
     9181 gggaaccgat tttaacaata tttgggtgta agggtgaagt ggagaaagca taactttgtc
     9241 gatatttcca aactctacta catcccctct caacattatc gcaatcctat cacttatata
     9301 gtatgcagtg gcaaggtcat gggttatgta taaaacagta acgttgtatt cttctttcaa
     9361 gtcactgaat aagttgacta tagacattcg caaagaggca tcaaccatag aaacgggctc
     9421 atcagctatt agcagagaag gccttgttat taaggctctc gctaccgatg ttctttgtag
     9481 ctgtcctcct gacaattcat tagggtatct accctgcacc tcatttaaag tcagtcccac
     9541 cgattttaag gcttcctcaa ctaatttcgc tttttctccc ttagtttttg ccaaattgta
     9601 atttcccgct gtttcgaaaa gataagaatc tacttttctc aggggattaa aagtttcgaa
     9661 ggggttttga aatatgggtt gaacttcttt catgtattct agtaattctt ctcttttctg
     9721 gatatggctg acgtttttcc ccttgtatgt tatttctccc gaagttggtt gttccagccc
     9781 cagaatcatc ctggccagcg ttgtctttcc actacctgtt tcccctgcaa aagttaatat
     9841 ttcaggtttt tcggaatcta tatcaaaact cacattatta actgccttta atttagtccc
     9901 taaaaatctt ccacctatat gaaagatttt tacaacactt ttaacttcta ataatttcct
     9961 ggacaatttc ctcaccttcc tctatttcag aggagtataa gaaacaagct atattatgag
    10021 attcctcata ctctatgagc ttaggtatat gcctcttaca tacgtctttc acataagggc
    10081 atcggggatg gaatcgacat ccaggtggag gatcagctaa agatgggggg ttcccgggag
    10141 cactggtcct tcgcctctta tctccaatcc tgggtaaaga ctgtattaaa aattgagagt
    10201 aagggtgttt cggagcggtg aataactctt ttttctcagc tatttccact atttttcccg
    10261 catacataat cgctatcttt tctgccacat tagcatgtac cgccatatca tgagttacta
    10321 tcagcatcgt atttttttgt tcttcatgta attgcttaag catctgtatt actcctcttt
    10381 gaaccacaac atccaaagct gtggtaggtt cgtcagcaaa tattatttta ggcttcagaa
    10441 tagttgccag agctattgta gttctttgtc tcatacctcc agaaagttgg tggggatagg
    10501 aatctaaaac ttctaaagga aggcccaggt attttatatg ctcttccata agtctttcat
    10561 attcctctct actttcactc tttttatgag cttttataac atcctcaaaa gtttttctaa
    10621 ttttcctcac cgggttcaga gtgctcatag agccttgggg aatataagat atatcttccc
    10681 actggacctt tcttagttgt tctctctcta atgtcaggag gttgaatttt ttaccgtcgt
    10741 aagaatagtt aacttgaccg ctcaaaaccc ggagaggagg ttttattata gctaaaagta
    10801 ctttgattaa agtacttttc ccgcagcccg actcccctgc taaccccaca atttcatttt
    10861 cttccattga gaatgtaata ttatctactg ctcggacatc tctttgtacc ccataagcat
    10921 gggtgatgta ataagcagag agattttcca cttccagaag cgacatctat aattaacctc
    10981 ccatctttat tctttgcaac cgggtcctcg gatccaaaaa ctcacttata ctcacggaaa
    11041 gcatatacaa agcaacaaaa agcaagacac aaagaattat aggggtaagc aaccaccacc
    11101 atattcctct aaagatggct tgatattgca tagcccagtg aattgtggtt ccaattgtag
    11161 gagtttccaa acttgttaag ccaagcactg acaaagtaat ttccattccc agcgcccata
    11221 gtatcgtatt tataaaattt gcgataaccc acggaataac aaaaggcaag tgttctcgaa
    11281 aaactacctt ccacatattc attcccgaaa aaacagatgt gtaagtaaat tctctctctc
    11341 ttaaacttaa gacttgtgcc cggacttgtt tgcctcccca gggccatgag aatacggaaa
    11401 gcgtcaatcc tagaacaaac atggtcattt ttccccttaa agagaaagag agaaagacaa
    11461 gaattggcaa agacggtaaa actatgaaac tatcatttat agacattaaa actttgtcca
    11521 gtgttccccc tctataccct gctatcaggc ccacaatagt accgataata gtagccacaa
    11581 ccgcggtggt tattcccaat acgaaagaat ttttcaacgc atgagtggtg ttccaaaata
    11641 tatcttgccc catcgagttg gttcctaaaa tatattgaaa actgggaggc ctgtccttgg
    11701 gaaccacact ccagcttcgg ggatcatagg gagaaaaaat cgataatata gctaaaaaaa
    11761 ccaaaataca taccacgata aaaccaaaaa gaaatttttt atctcgttta aggagatctt
    11821 ttaagataac aaacatctca tcaccttatc tgtatcttat tcttggatca aacaaaggat
    11881 acaataaatc tataaccaag gaagcagtag ctatagctat tattgaaaaa accgcaatcc
    11941 ccatcatcaa attaaagtct ccgttagtaa tggcattttt taataataac cccacccccg
    12001 gataagaaaa agctacctca gttataaccg ctccattaaa tatacctcct atttgtagag
    12061 ccaaagcagt tacctgggga agtatggcgt ttctcatgac ataactgaac agcaccttat
    12121 gtctgggcag gcctgcaaat tctatatagg taacataatc ttcagctacc acattggagg
    12181 tcatcgacct catacttaaa aaccaccagc caattccaat gattatgaga gaaatagcag
    12241 gcaatatgct atgccttaaa acatctacga tgaaagccca gctcagagag ggttgtattc
    12301 ctatactata gcctcccatc attggaaaca ctggaaatat atatacaaac aaaattacta
    12361 aaatcaatgc cataacataa tacggaatgg ggtatatgct catagctatg atagctaaca
    12421 cgttagacca tttttcgtcc ttaaaatatc ccgccaatcc tccaaggata tttccaatta
    12481 tccaggatat taacaaagta gttaaaagca aaaaaacagt ccagggaaga gaatttgcaa
    12541 taatttctat gaccggagtt ggaaacatgg ataaagatgg tccgaagtcc agagtaaaga
    12601 gatgtttcca aaaggcaaaa tattgttcca gcagacttcc ttccagcccg tatagctctt
    12661 tcaaagtatt ttgcagactt tcaactatct ccggttccat atatattccc ccgtatgagg
    12721 tcagacggtt aatagcggtt tgtacagggt ctataggagt taatctggga attataaaag
    12781 ttatggttat cccaataaaa ataaccacta cgtactgaat taagcgagga ataatataag
    12841 tttttaaggc attcacaaaa tttttccctc cactttttaa taacgctatt tttgggagct
    12901 atgcaacctg ctttaagagc gttattataa ttaactttat acccattccc acttgatctt
    12961 aaacttcccg gcattatctt cttcgcatga gctatataca ccgtgcttta tcaaaaaatc
    13021 ccgttaaaaa tttctcagtt tatagatttt acccttaaat ttagaatccc catttctaag
    13081 ttgagcccgc tcaaatgagc gggctcaatc agctctggat tagaagaact tattctcttc
    13141 cggtaggctc aatcctggga aacatgaatt tgaattcagc ccaccaacta actggtgcca
    13201 tatagggact atcagcactg gggtaatttg tccagtagta attatcgtta gcgttgaatt
    13261 ttttgttaat taccatagga atagtgggca tctcttctac agcgagttta taaaattcct
    13321 tcgccagctc tattatttta ggatcaccag gatatagagc gcccatttca tctattattt
    13381 cgtctactct ctcgggaact ttccatcgat aattatttgc ggtagtaaca ggagttccta
    13441 agggctccag aaaatcggat ttaaaaccat ttataaacgt ccatatatct gggataccag
    13501 aagaggctaa catcccaccc cagaaagatc ccgcatcata atctcctatg gcctgcctgg
    13561 accataatgg acctgcttct aaagtttcaa catttacctc aactccaaat tttctccatt
    13621 gatctgcaac tgcaaaggca agtctggtgg catctatttc aaaatcggca ggagctatta
    13681 tacttatgtt ccagggagta ccatcaggta atagccattt tccgttttga tctttagaga
    13741 atccattttt caaaagtaaa tcttctgctt tttgaggatc gtatttccac caaccgattc
    13801 cccagacagc ttcaggatct tctacttcta tgttatattg ctcttcagcc cactctgcaa
    13861 ttctataagc tacttctgta tcatatggtt tatagtcacc atctatggtg aaatcctcga
    13921 gccacggctc taatggttta aagtaaattt ccgtgaaata ttgagtggga caggagtgca
    13981 gaggtgatac cctctctatt cctgcatatc ccgatataat gatttcttct atgtttgttg
    14041 ccagggttag cgcccatctt acatctttaa tgtcaaaagg atatctggca ttattgagga
    14101 acattcctct tgagtttaca tcatccatcc atgcccatgg gaaatcccga taccatacaa
    14161 ccgagtgttc atttctttct cttaaaactt cccatgcctc aggagttaaa tcaaaaatca
    14221 tgtccagttc atgcctggct tgagccatca cttttctatc atctgtaccg tagtagatga
    14281 agagtacata gcttggtttt ggctctccat acaattgccc aacacttgtc ctttcccagt
    14341 catcacgttt tttaaacagg aaccagtacc cctgagagtc ataactatca agcacatacg
    14401 ggcccaatga gacagggggg ttgaaatcga aggtcaaggg attctcaatt tcttcccata
    14461 tgtgtttggg ctgcataaaa agagctccgt aaaccagaga agtaaatatg ttatgcattc
    14521 tagaatatga tttggtaagt tc
//
LOCUS       DAOPSS010000031        13865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_3729_length_13865_cov_7.927444, whole genome shotgun sequence.
ACCESSION   DAOPSS010000031 DAOPSS010000000
VERSION     DAOPSS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 13865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13865
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3729_length_13865_cov_7.927444"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..424)
                     /locus_tag="PKN31_03230"
     tRNA            complement(337..424)
                     /locus_tag="PKN31_03230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Ser,seq:tga)
     gene            complement(465..1562)
                     /locus_tag="PKN31_03235"
     CDS             complement(465..1562)
                     /locus_tag="PKN31_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA98976.1"
                     /translation="MEKIGIGIVGFGFIGRVHALAFSCIPYCYHDLPCLPEIRGIATS
                     RPETAQKVKEETGVAFVTSSFSELINREDIDIVVVALPNVLHQEVVNKAARAKKAIYC
                     DKPLSSNLKGALAMEEVVKEENVLLGVVFQNRFVPALWRTKELIQEGFLGEILRFRAL
                     YLHSGYLDVERPWSWRLDEELSGGGALLDLGSHLVDLLFYLTGEEVNIKASWGKTFIP
                     ERNGRDVKVDDWSLVLFDLADGGEGSIESSRISTGSADELRLEIEGRKGAIRYNSMQP
                     NYLEIYDLKDKASPLGGNRGFKALETISQFPPPFALPGKFPLGWINAHMACAHDFLLR
                     FAGRDARGATLEDGIKVQKFIEDAYHSMSGG"
     gene            complement(1566..2849)
                     /gene="serS"
                     /locus_tag="PKN31_03240"
     CDS             complement(1566..2849)
                     /gene="serS"
                     /locus_tag="PKN31_03240"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOA98977.1"
                     /translation="MLDWKWIKENKESVEEALQKRGMDSGFLEELFSWDEERRAAQRE
                     VDILRQKRNEISRLIASLKREGKDPQDALQEGEKIAGKIKEEEEKLREAEEKWREKLL
                     FLPNIPHPTVPVGKDENDNQEVKQWGEPRHFSFTPRPHWEIGEGLDILDFERGAKIAS
                     SRFTVLKGEGAFLERALTNFMLDLHTQKHHYREIFPPFLVNTKSLIGTGQLPKFYEDL
                     YHCQDDLHLIPTAEVPVTNLHAEEILREEELPLYYVAYSACFRREAGSYGKDVRGLIR
                     QHQFNKVELVKFALPENSYEELETLLQDAEEVLQILDLPYRVVVLCTGDLGFSASKTY
                     DIEVWIPSQNRYREISSCSNFEDFQARRANIRFRDQGGKVRYVHTLNGSGLAVGRTLV
                     AILENYQEEDGTVTIPEALRPYLRGLKSITPQFEA"
     gene            complement(2863..>3198)
                     /locus_tag="PKN31_03245"
     CDS             complement(2863..>3198)
                     /locus_tag="PKN31_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOA98978.1"
                     /translation="VGQPVLIPGDMGTGSYILVGTELAMQETFGSTCHGAGRVMSRSE
                     ADRRSRGRNIANELKERGVLVQAESKATLREEIPEAYKDVGEVVEVVERAGISKKVVY
                     ARPLGVIKG"
     assembly_gap    3199..3298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3299..3798)
                     /locus_tag="PKN31_03250"
     CDS             complement(<3299..3798)
                     /locus_tag="PKN31_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOA98979.1"
                     /translation="MAEAWKGPLQKVDDWRWLIPRSYKKGMLTDGMIYASEKMISAIR
                     KDQAPEQVANVACLPGIVGRSLAMPDIHWGYGFPIGGVAATQEKGGVISPGGVGFDIN
                     CGVRVIKTNLTEEEVRPHLEKLLSLLFTNIPSGVGSKGKLSFSIPELEKVLEKGARYI
                     ISRGFG"
     gene            complement(3816..4235)
                     /locus_tag="PKN31_03255"
     CDS             complement(3816..4235)
                     /locus_tag="PKN31_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOA98980.1"
                     /translation="MNKFEILDHTADVGLVAWGETKEEVFANAALGMFSLICNVEEVN
                     GGFTTQVEVEGSDYEDLLVTWLNELLYLFEAEEVLLKEFNIQELGQYYLKAQVEGEPL
                     DTQKHHVQRVVKACTYYEVKVEEIRPQLWRAQVYFDI"
     gene            complement(4245..5195)
                     /locus_tag="PKN31_03260"
     CDS             complement(4245..5195)
                     /locus_tag="PKN31_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOA98981.1"
                     /translation="MSEKIKILGAAGIPKEMVERLEQEIGPVEVLSSDSEEEIIKAIQ
                     DKQVFVVRSKPKVTAHIIQNAPQLKVIARPGVGTDNIDRKACEERGIKVVNTPEASAS
                     SVAELTIGLAIALLHYIYPTCHSLKEGKWLKGEYTGKTIEGKTWGIIGFGGIGRRVAE
                     IVKVLRAEVLAYDPYVPDEEFTKRGIKRIARLEELLEQSDIVSIHVVLNQETEGMFSE
                     EVLHKMKKGAYLINTSRGKVINQKALEKALEEGYLGGAALDVYEVEPPMDQELLSLPN
                     LICTPHIGGSTEEAFLNATEILVRKIKEIFHIKETAPLGS"
     gene            complement(5188..6216)
                     /locus_tag="PKN31_03265"
     CDS             complement(5188..6216)
                     /locus_tag="PKN31_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOA98982.1"
                     /translation="MGGLQDVFQTKNEVLILPGGETGALEGAVVNFFSPQDKVLFGIM
                     GESGNRWAQIAERFGLQVLRLGSERGEVITAPDVELLLRSEAGKDIKGVFITHNEIST
                     GVVADIERLGKVCQKHHLLFLVDALNSLGVVDLKTDEWGVDVVVASPKNLIAPDPYLS
                     LFSVNDKAWKYNAKARCPRYYWDLAQTRKYFHHPTPESPCIPVSFLFGLDETVQTIKK
                     EGLENIFSRRQKLSQMLRRGIEALGLELLVKDEYIASPSVTAIKVQEEVEAEQITSFL
                     KEEGITTIGGETKLQGKIILIEHSDCFCAFDIANIIATLGRALKAQGVAVDLAKSLTR
                     VWEVYDNE"
     misc_binding    complement(6388..6593)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6802..7290)
                     /locus_tag="PKN31_03270"
     CDS             complement(6802..7290)
                     /locus_tag="PKN31_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOA98983.1"
                     /translation="MAQKTRELVKDIADELIRLLNKAYADELIAYFYYKTAALLVKGI
                     HANTIADQLEEIAQEELEHSEELAERIIQLGGEPLDDWDAITKNANYPKIDIPEDRSD
                     YVGILKAVHVAEQGAIGVYDDLMKFLQSSQTKDPVTTHLIRHIMGEEMHHEEEIETLL
                     GL"
     assembly_gap    7619..7718
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9211..10995
                     /locus_tag="PKN31_03275"
     CDS             9211..10995
                     /locus_tag="PKN31_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA98984.1"
                     /translation="MDVFKRLWTYVRPYKLQFILTTIVLLAAVGVGMVGPYLTGLLID
                     RVIRAGELDLLRLLTVGLVTASVVGSVLQYTHGYSFEDLSQKVIYSIRHDMYNHLQQA
                     SFSFYDEAHTGELMSRLTGDLEGVRVFMVGGFPMLVSSMVTFVGVTIVLFNMNAKLTL
                     VCLSFSPLLAITAVSFEKKVKQAHSDIRHQMAAFNRFLQENITGIRVVKAFAREKYEI
                     ERFRAENSKVYEKNMIAGLLNGRFGPLLEFLSSMSVVALVSYGGWLVMRGEMTVGILV
                     AFNGYLWSLIWPMRQLGMLLNLTGWAISSGQRIFNLLDTNVSMPVKPNAYKPDKVRGD
                     VRFDKVTFKYGGDIVLEDINIDAPAGKKVAIMGATGAGKTSIVNLICRFYDPQKGRVL
                     VDGVDVRDWDLKTLRRNVSVVMQETFLFSDTIANNIAYGNDNASMDEIVAAAKAACAH
                     DFIMEMPQGYDTIVGERGVGLSGGQKQRIAIARALLINAPVLILDDATSNVDMETEHE
                     IQQALKRLMVGRTTFIIAHRISAVKDADEIIVLEKGRIVERGSHDQLLQARGFYYEIF
                     KEQYKDLVAENVAPELVTDDEDEVLWLG"
     gene            10983..12770
                     /locus_tag="PKN31_03280"
     CDS             10983..12770
                     /locus_tag="PKN31_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA98985.1"
                     /translation="MARVNRFFDDEPLRRPFNKDQFIRLLGYLGPFKKHLLAAFMLML
                     VATLFGLAGPYIIKLAIDNYIAAGDIKGMIYLTLVFAALTVAIYFINRKRVTMMTGMG
                     QRMLYNMRMDLFQHIEGLSFTFFDSRPAGKIMMRLVNDVNSLNDLLTNGLINVINDFI
                     MLIAIIVIMFSMNVRLTLVSFLLLPALVAIMFGLRSVIRERWQIVRQKNSNMNAYLQE
                     NISGMRVTQAFVQEDVNEKIFLELNGDIKTAHMNAIRVNNAFWPLLNIMGAVGNALVF
                     VVGVGMVTRGEVTVGTLVAFTSYIGRFWMPLSTMSNFYNQLLIAMASAERIFEILDTE
                     PEVADKPDAIVLPPIKGHVEFDHVYFYYEPDKPVLRDVSFVVQPGENIALVGPTGAGK
                     STIINLLARFYDVTGGRVLIDGFDVRDVTLESLRSQLGIVLQDTFLFSGTIMDNIRYG
                     KLDATDEEVIAAAKAVCADDFIREFKNGYFTEVKERGSRLSVGQRQLISFARALLANP
                     RILILDEATSSIDTHTERLVQVGLERLLEGRTSFIIAHRLSTIRNADRIMVVDEGQIK
                     ESGTHEELMATGGIYRELYTSQFKWLLAG"
     gene            complement(13244..>13865)
                     /locus_tag="PKN31_03285"
     CDS             complement(13244..>13865)
                     /locus_tag="PKN31_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA98986.1"
                     /translation="VAANKLTTAANAILLQYGAPVYVAFLGKWFLGEEVAREDWWAVG
                     AVLGGMVLFFFDQLQWGNLVGNLLAVLSGWTFAFLIIFMRMQKDSSPLESVLLGNIFT
                     ALIGLPFMWQGAPPPGSWATLIFLGTVQLGISYILYSIAIKGVTALEASLIPIIEPIL
                     NPLWVFLFMGEVPGKWAMVGAGVILISIVLWYLEKSVFRFCRNRGG"
BASE COUNT         3631 a         3023 c         2981 g         4030 t
ORIGIN      
        1 ccagggaccg accggttatg agccggctgc tctaccgctg agctatgggc cctcaaggcg
       61 aaataaatat tagcatatta ttgctttctt atcaagcttt gcccaggata ggataaaaat
      121 acatatttaa ccaccaggtc tttgcaatct cgcttccctt ttgtttttgt aactaccgag
      181 agatgttttt ctctttcggt agctttcatg tctgatagct ttttgtttac gctctgaggg
      241 gttatgaggg ggggggattt atccccttta taagttcttt ttatctcacg ctgcaggata
      301 ttctttttat tggtaggttg gtaggaggtt ttctgctggc ggagggggcg ggactcgaac
      361 ccgcatcgcc tttcggctta ccggttttca agaccggctc cttgccaatt cgggacaccc
      421 ctccccgttt ccttgtttat tatactttaa aagaagagct ttcttcaacc cccggacatg
      481 gaatgatagg catcttcgat aaatttctgt acttttattc catcctctaa agtagctccc
      541 cgggcatctc ttccggcaaa gcgcagtaga aagtcatgag cacaagccat gtgggcattt
      601 atccacccca gggggaattt tcccggaagg gcaaaaggag gagggaattg agaaatagtt
      661 tccagtgcct taaaccctct gtttcctccc aggggagaag ccttatcttt taggtcgtaa
      721 atctcaagat aattgggttg catagaatta taccggatag cgcctttcct tccctctatt
      781 tctaatctca gctcatcagc actccctgtg gatatccggc tactttctat gcttccttca
      841 ccaccatctg ctaggtcaaa aagaaccaaa gaccagtcat ctacttttac atctctccca
      901 tttctctcgg ggataaaagt ctttccccag gaagctttga tgtttacttc ttctcctgtt
      961 aaatagaata agagatccac aagatgagag cctaaatcca gaagtgcccc tccaccggat
     1021 aactcctcat ccagacgcca gctccaggga cgctctacat ctaagtagcc ggagtgaaga
     1081 tacaaagctc ggaaccggag aatttctcct aagaaccctt cctgaatcaa ctctttagtc
     1141 cgccaaaggg ccggaacaaa gcgattctga aaaaccactc ccaggagaac attctcttct
     1201 tttaccactt cctccattgc cagggctcct tttaaattag aggagagagg tttatcgcag
     1261 taaatagctt tcttagcgcg agccgctttg tttactacct cctggtgcag gacgttggga
     1321 agggcaacta ccacgatatc gatatcctct cgattgatta gctcagaaaa agaagaagtg
     1381 acaaaagcaa cgccggtctc ttctttgact ttctgggcag tttcaggacg agaggtggct
     1441 attcctcgaa tttcaggaag gcagggaagg tcatgataac aataaggaat gcaagagaaa
     1501 gcgagggcat gaacccgacc gataaaacca aaccccacta tgcctattcc tattttctcc
     1561 atttatcaag cctcaaattg gggggttata gattttagtc ccctgagata aggccggaga
     1621 gcttcgggaa tggtcactgt cccatcttct tcctggtaat tttccaggat ggctaccaga
     1681 gttctaccca ctgccaatcc cgaaccattt agagtgtgca catagcgcac tttgcctccc
     1741 tggtcgcgaa agcggatatt ggctctccgg gcctggaaat cttcaaaatt actgcaagaa
     1801 gaaatttccc ggtatctatt ttgagaagga atccatacct caatatcata ggttttgctg
     1861 gcggaaaaac ccagatcacc ggtacacaat accaccaccc gatagggaag atcgagaatt
     1921 tgcaacactt cttcggcatc ctgaagaagt gtctccagct cttcatagga gttttcagga
     1981 agagcgaact tcaccaactc tactttgtta aactggtgtt gacggattaa gcctctcaca
     2041 tcttttccat aagagcctgc ttctcttcga aaacaggcac tataagccac gtaatataag
     2101 ggaagctctt cttcccggag gatttcttcg gcgtgtaaat tggtaaccgg gacttctgcc
     2161 gtggggatga ggtgaaggtc atcctgacaa tgataaaggt cttcataaaa tttgggaagc
     2221 tgcccggtgc caattaaaga tttggtgtta actaaaaaag gaggaaatat ttcccggtag
     2281 tggtgttttt gggtgtggag gtctaacata aagttagtga gtgctcgctc caggaatgct
     2341 ccctcgcctt ttaatacggt aaagcgagaa gaggcaattt ttgctcctcg ctcaaaatcc
     2401 agaatatcca atccttcccc aatttcccag tgaggacggg gggtaaagga aaaatgtcgc
     2461 ggctctcccc actgttttac ttcttggttg tcattttcat cttttcctac tgggactgtg
     2521 ggatggggaa tgttggggag gaagagcagt ttctccctcc atttttcctc tgcttcccgg
     2581 agtttttcct cttcttcttt tatttttccc gctatttttt ccccttcctg aagtgcatct
     2641 tgagggtctt tgccctctct tttcagggaa gctatcagcc gggaaatctc gtttctcttc
     2701 tggcgcaaga tatctacttc tcgctgtgca gcacgtcttt cttcatccca ggagaaaagc
     2761 tcctccagaa acccggaatc cattcccctt ttttgcagag cttcttccac agattctttg
     2821 ttttctttta tccatttcca atctaacatt ttatttttcc tctcatcctt tgattactcc
     2881 taaggggcgg gcataaacca ctttttttga aattcccgct ctttccacca cttctactac
     2941 ttctcctaca tctttgtacg cttccgggat ttcttctcgc aaagtggctt tactttccgc
     3001 ttgaactaaa actcctcgct cttttaactc attagctatg tttcttcctc tagagcgccg
     3061 gtcggcttcg gagcggctca tcactctacc tgccccgtga caggtggagc cgaaagtttc
     3121 ttgcatggct aactctgttc ccacgagaat ataggagccc gttcccatgt ctccaggaat
     3181 taaaactggc tgacccacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     3301 tccaaaaccc cggctgatga tgtagcgtgc tcctttttcc agaacttttt ctaactcggg
     3361 gatggagaag gagagtttgc ctttggaacc tactcccgag gggatattag taaaaagaag
     3421 ggaaaggagt ttctcaagat gtggccttac ctcttcttcg gtgaggttgg tttttatcac
     3481 ccgcactcca cagttgatat caaaacctac tcctccagga gaaatgactc cccccttctc
     3541 ctgggtagct gccactccgc caatggggaa accatatccc cagtgaatat cgggcatggc
     3601 taaagagcgt cccacaattc ccggaagaca ggctacgttg gccacctgct ccggtgcctg
     3661 atctttgcga atggcagaaa tcatcttttc actggcataa atcatgccat cggtgagcat
     3721 tccctttttg taacttctgg gaattaacca ccgccagtcg tctacttttt gtaacggtcc
     3781 tttccaggcc tcagccatgg tataagtcct ccttttcaaa tatcaaaata aacttgggct
     3841 cgccagagct ggggtcttat ttcttctacc tttacctcgt aataggtaca ggctttgact
     3901 acccgctgaa cgtggtgttt ttgagtatcg agaggctctc cttctacttg ggctttgagg
     3961 taatattgtc ctaattcttg aatgttaaac tctttcaata gtacttcctc agcttcaaaa
     4021 aggtaaagaa gctcgttgag ccaggtgacc agtagatctt cataatcact tccttccact
     4081 tctacctggg tagtaaatcc tccgttcact tcctctacat tgcagatgag agaaaacatt
     4141 cccagagcgg cattggcaaa tacctcttct ttggtctctc cccaggctac taatcccaca
     4201 tcagcggtat ggtctaaaat ttcaaattta ttcatttcta tccctcaaga gcctaaagga
     4261 gctgtctctt tgatgtgaaa tatttcttta atttttctaa ccagaatttc tgtggcattc
     4321 aaaaaagctt cttctgtgct ccccccgata tgaggagtgc aaatcaaatt gggaagagag
     4381 aggagttcct ggtccatagg aggctccacc tcgtaaacgt ctaacgctgc tcctcccaga
     4441 tatccttcct ctagtgcttt ttctaaggct ttctgattta taactttccc tcgagaggtg
     4501 tttatgagat atgctccctt tttcattttg tgtaaaactt cttcagaaaa cattccctct
     4561 gtttcctgat taagaactac atgtatggac acgatgtcgc tttgctccag cagctcttct
     4621 aatcgcgcaa tcctttttat tcctcttttg gtaaattcct catcaggaac gtaggggtca
     4681 taggccagga cttcagctcg tagaacttta actatttccg ctactcgccg accgatgcct
     4741 ccgaatccta ttattcccca ggttttacct tctattgttt ttcctgtgta ttctcccttt
     4801 aaccattttc cttccttcag ggaatggcag gtaggataga tatagtgcag aagggcgata
     4861 gccagcccga tggtgagttc tgctacgctg gaagcagaag cctccggggt gttgaccact
     4921 ttgatacctc gctcttcaca ggctttgcgg tcgatgttgt ccgttcccac tcccggtcga
     4981 gctatcactt ttagttgagg agcattctga ataatgtgag cggtaacttt cggtttggag
     5041 cgaacaacaa agacctgctt atcttgaata gcttttatga tttcttcctc actatcggag
     5101 gatagaactt ctaccgggcc tatttcttgt tctaaccttt ctaccatttc tttaggaatt
     5161 cctgctgctc ccaaaatttt gattttttca ctcattatca tacacctccc aaactcgggt
     5221 taagctttta gctaaatcca ccgccactcc ctgagctttt aaggctctac ccagagtggc
     5281 aattatatta gcaatatcga aagcacaaaa gcaatctgaa tgttctatta aaattatttt
     5341 tccctgaagt ttagtctcgc ctcctattgt tgttattcct tcttccttga ggaaagaagt
     5401 gatttgttct gcttctacct cttcttgaac ttttatagcg gtaaccgagg gagaagcaat
     5461 atattcatct tttaccagta actctaagcc taaagcttct attccccgcc ttaacatctg
     5521 ggagagtttt tgccggcggg aaaaaatatt ctccaaaccc tcttttttga ttgtctgcac
     5581 agtttcatcc agaccgaaaa ggaaggatac cggaatacag ggactttctg gagtagggtg
     5641 gtggaaatat tttcgcgtct gagccaaatc ccagtagtaa cgaggacaac gagctttggc
     5701 attatatttc caggctttat catttacgct gaaaagggaa agataaggat ctggagctat
     5761 caggtttttc ggggaagcaa ccactacatc tactccccat tcatcggttt ttaaatcaac
     5821 aactcctaaa gaattcaggg catctactaa aaagaggaga tggtgtttct gacacacttt
     5881 ccctaacctt tcgatatcag ctactactcc ggtggaaatt tcattatggg taatgaaaac
     5941 tccttttatg tcttttcctg cctcacttcg caagagaagc tccacatccg gggcagttat
     6001 tacctcaccc cgttcacttc ccaggcgaag cacctgaagg ccgaaccgtt ctgcaatctg
     6061 agcccatcga tttccgctct ctcccattat tccgaatagt actttatcct gaggagagaa
     6121 aaagttaacc actgctccct ctaaagcgcc agtttctccc ccgggaagga tcaaaacttc
     6181 gttcttggtt tgaaaaacat cctgcaaacc ccccattgcc ctcccgagga gtgctttaaa
     6241 agcttcatcc tggtaacagg tgattttctg cgctctacct tgaaacgccc tccggggggt
     6301 tggggtagag cagggagcta tcaaaaaagg tgttttcact acgtttctcc ttcctcgctt
     6361 tatagtatat aacaaaaaac tctcgccccc aggggcgaga gtttctcgct gtgccaccct
     6421 cattcggtgg ttttatccac cctcgtttat ttaacgctta aggcggaggg aattacttgt
     6481 tttccttccc tcggctccag agcggattca ggaagaagaa ataccggctt ccacataacc
     6541 cggctttctg ttatttctcc tttcccccta ctactctctt tctcagccgt ttcttattct
     6601 taatgtacaa gtaatatagc acaccccagg gtaaagtcaa gaagggagag gctgcttttc
     6661 tgacactatc atctttggat taaaaaaccc ccaggtgaga aaaaagtggc ttacaactct
     6721 gataactttt ggaggctaca tgaggagggt tatctaaaaa aaagaagggg ttaaaaaccc
     6781 cttctttttt ttaggccttg cttacagtcc cagtaaagtt tcaatttctt cttcgtggtg
     6841 catctcttct cccattatgt gccggatgag atgtgtagtg acagggtctt tggtttgaga
     6901 agactgtagg aatttcatga ggtcatcata aactcctatg gctccttgct ctgccacgtg
     6961 tactgctttc agtattccca cataatcgga tctgtcttca ggtatatcta ttttgggata
     7021 attcgcgttt ttggtaatag catcccagtc atccagcggt tctcctccca gctggataat
     7081 tcgttcggct agttcttctg agtgttcgag ctcttcttgg gcgatttcct ccagctgatc
     7141 ggcgattgta ttggcatgga tacctttgac caatagagct gcggtcttgt aataaaagta
     7201 ggctattagc tcgtctgcgt aggctttgtt gagcaatcta attagctcgt cggcaatgtc
     7261 tttaactaac tcccttgttt tttgtgccat tcagatccct ccctcttaaa tgttttattg
     7321 aattacgctc actatactac aaacccattt tagcctaatc cgactcttga tgtcaaacgc
     7381 taactggagg ctgtttgaaa aacagacagc ccacggtgta agcgaaaaac atgaaggaca
     7441 aaaagtaaga gagcaaaaac cactgaggaa agaacatttt cctcagtgct cgcggcaccc
     7501 ctctttttcg tcactctgaa tgtatttatc aggagtccat gttattaagg acaaaagtat
     7561 gtggatttcc gatagaaagt ttttaaaatg acggaagggg gagggttcct tttctcatnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ccgggagaaa agtcctctct
     7741 cagcgaaaag gcgaactcgc attaactgtg atagatgtca cgatgcagat gacaagaatc
     7801 tccatctctg ggagagaaaa ttcttgtcag agagtattat ctcttaccat ggcattcatg
     7861 atagcaagaa gcttcctcgt gcgagagttt agttacttta gaggcttttt ctttctctat
     7921 aagtgttcat ggaattctct aattaccggg ttacacctta tggctgaaag ggtattaaga
     7981 aagcatattc agctatgaga tgagcatctg tagcatcagt tttagctagc ctctctgaga
     8041 ctcgaggtag gcccttactt ctggtggggc tgactactga cataggggaa ttttgagagg
     8101 atgaaaccgc tactaaaggc ctttcatatc ctcctctcct ttcactaatt aaggaaggat
     8161 ttatcttctg gagagcaaga aggagcttct ctattccttc tccggtatta gggaatgtgg
     8221 tggatttctt tgaaccataa acttatggat gtctgattaa agatttcagg catagtcaag
     8281 tccccttttt gccgtagaag tgggcggttt cctcatcatc tcaacagttc tcatagtctt
     8341 tctttcccgg ccttgagctt gaggaggact ttctattttc ccgagcacat aagatggctc
     8401 agagctatca cttgctggta attatatcca gcctgtcaat tttggcggta tttattttcc
     8461 taccccctta gctttaaatt tagctaggtt ctttttttaa aataatttgt ttttgtgttg
     8521 attcggactt ttattatatt tggtcataga ctaaaatgta agggacctct tgagctggaa
     8581 agaagagcaa gctgtattta tcctgtagat gtacaggatg gagagagtaa ggaaatgggt
     8641 agtattatga tgcagcacta aaggttggta taacaaaaga ggtactgaat gaggggagac
     8701 tatgtaaaga gtatccaggt gagtacacag atacgccccg accagataat aaggcagaag
     8761 aaataactat ttaaggaact accattgata tttagtgata agaaagcaag gctgaacatt
     8821 aaggaaactg gaaagaggac cacgttatag aaacagacaa taaggaaaag cataaaatga
     8881 tagagctaat gaataccgaa ataataatac atagacaatt tgaacttatg gaacctcctt
     8941 gctcttcctg ttgctgtggg tagtaaaaga gggtgaggat tataatctac ttcttatgaa
     9001 aatcagtaga gatggcagag attggctatt tgatagtatt ttcgcagaga ggcttcgcaa
     9061 atcctctgaa cccggaaccc ctccgggaag tgtactcagg gccggggggg gagagaaaag
     9121 aaaaacaaac aggctggaac aatttacctt aaaaatctct ttttgtcttg acgtcgggat
     9181 ctaccaatga tgtaagaaga gggcaggttt atggatgttt ttaagcggtt gtggacttat
     9241 gtacgacctt ataaattaca gtttatactg actactattg tccttctggc tgcggtaggg
     9301 gtgggtatgg ttggccccta cctgacaggg ttattgatag acagagtcat aagagccgga
     9361 gaacttgact tattgcggtt attaactgtg ggtcttgtga cagcaagtgt tgtgggatct
     9421 gtactgcaat atacccacgg ctattccttt gaggaccttt ctcaaaaagt catttattct
     9481 attcgccatg atatgtataa tcatcttcaa caagcctctt tttcctttta tgatgaggca
     9541 catacaggtg agcttatgtc gcgcctgact ggtgacctgg agggggtacg ggtctttatg
     9601 gtgggaggtt ttcccatgct ggtatccagc atggtaacct ttgtaggtgt gactattgtt
     9661 ctttttaata tgaatgctaa gctaaccctg gtatgtctat ctttttcgcc acttttggct
     9721 ataacggctg taagctttga aaaaaaggta aagcaagcac atagcgatat acgccaccag
     9781 atggcagcct tcaaccgctt tctccaggag aacataaccg gcatacgagt ggtgaaagct
     9841 tttgctcgtg agaaatacga aatagagcgg tttcgagctg agaatagtaa ggtctatgaa
     9901 aaaaacatga tagcaggatt gctgaacggc cgttttggac cactgcttga atttttaagc
     9961 agcatgagtg tggtggcact ggtatcctat ggtggatggt tagtaatgag aggggaaatg
    10021 actgtgggca tattggtagc ctttaacggt tatctctggt ctttaatatg gcctatgcgc
    10081 cagctgggaa tgttactcaa cctgactgga tgggccatat cctcgggtca gcgaatattc
    10141 aatcttctgg atacaaatgt ttcgatgcca gtcaagccaa atgcctataa accggataaa
    10201 gtgcgaggcg atgtacgttt tgataaggta acttttaagt atggtgggga tatagtgctt
    10261 gaagatataa atatagatgc tccggcgggc aaaaaagtgg ctataatggg tgccaccgga
    10321 gcaggtaaga cctctatagt aaacttgata tgtcgtttct atgatcctca aaaaggacgg
    10381 gttctggtag atggtgtgga tgtaagagac tgggacttaa aaacattgcg ccgcaatgta
    10441 tcggtggtca tgcaagaaac atttctgttc tctgatacca tagctaataa tatagcctat
    10501 ggtaatgata atgcatcaat ggatgagatt gtagcggcag ctaaagcagc ttgtgctcat
    10561 gatttcataa tggagatgcc tcagggctat gacacgatag taggtgaacg aggagtgggt
    10621 ttgtcaggag gtcagaaaca acgtatagcc atcgcccggg cattactcat aaatgcgcct
    10681 gtgttgatac tggatgatgc cacatccaac gtggatatgg aaactgagca cgagatacag
    10741 caggcactca aacgccttat ggtgggccgt actacgttta ttatagccca ccgcatctcg
    10801 gcagtaaaag acgctgatga gattatcgtt ctggagaaag gccgtatagt tgagcgtggt
    10861 agccatgatc aactgctcca agccagaggt ttttattatg agatatttaa agagcaatat
    10921 aaggacctgg tggcagaaaa cgttgctcca gagttagtga ctgatgatga ggatgaggta
    10981 ttatggctag ggtaaataga ttttttgatg acgaaccact ccgtaggcca ttcaacaaag
    11041 atcaatttat tcgtctgtta ggatatttgg gtccttttaa aaaacacttg cttgcggctt
    11101 tcatgcttat gctggtagca acgctatttg ggttagccgg cccttacata ataaagttgg
    11161 ccatagataa ttacatagcc gccggtgata taaagggcat gatttacctt actctggtat
    11221 ttgctgctct tactgtggcc atttacttta taaaccgcaa gcgtgttact atgatgaccg
    11281 gtatgggtca gcgcatgtta tataacatgc gtatggattt atttcagcac atagagggtc
    11341 tgtcgtttac cttttttgac agccgaccag caggtaaaat catgatgcgg ttggtaaatg
    11401 atgttaattc tctaaacgac cttctgacca atggtctcat caacgtgatc aatgatttta
    11461 taatgttaat agctataatc gttatcatgt tcagtatgaa tgtgcgtctt acactggtat
    11521 cgtttctcct gttgccggct cttgtggcaa taatgtttgg tctgcgcagt gttataaggg
    11581 aacgctggca gatagtgcgg cagaagaact ccaatatgaa cgcttatttg caagaaaaca
    11641 tatcaggtat gcgtgttact caggcctttg tgcaggaaga cgtgaatgag aagatatttt
    11701 tagagttgaa tggtgatata aaaacagctc acatgaacgc tatacgggtc aataacgctt
    11761 tctggcctct tttaaatatt atgggagcag tgggtaatgc gctggtgttt gtggtaggag
    11821 ttggtatggt aacgagagga gaagtaactg tgggtacgct ggtggctttc accagttata
    11881 taggccgatt ctggatgccg ctttccacca tgagtaactt ctacaatcag cttctcatag
    11941 ctatggcgtc agctgagcgc atatttgaaa tactggatac tgaacccgag gttgctgata
    12001 aaccagacgc tatcgtgctt ccacctataa aagggcatgt ggaattcgac catgtctatt
    12061 tttactatga accagataag ccagtactga gagatgtaag ttttgtggtt cagcctggag
    12121 agaatatagc attagtagga ccaactggcg cgggcaagtc tactataata aatctcctgg
    12181 ctcgtttcta cgatgtaaca ggaggaaggg tattgataga cggattcgat gtccgggacg
    12241 taacgctgga gtcgcttcgt agtcagctgg gtatagtgtt gcaggatact ttcctttttt
    12301 ctggtactat aatggataac atccgctatg gtaagttaga tgcaacggac gaagaagtaa
    12361 tagcagcagc taaagcagta tgtgctgatg atttcataag ggaatttaaa aatggttatt
    12421 ttactgaagt taaagaacga ggttccagac tgtcggtggg gcaacgtcaa cttatatctt
    12481 ttgctcgtgc actcctggct aacccgcgta tacttatatt ggatgaagct acttcaagca
    12541 tagatacaca tactgaacgc ctggtacaag tggggttgga gcgcctgctg gaagggcgca
    12601 cttcgttcat catagcccac cggctatcta ccatacgcaa tgccgatcga ataatggtag
    12661 tggatgaagg gcaaataaag gaatcaggta ctcacgaaga gttaatggcc acaggtggta
    12721 tataccggga gctgtataca tcgcagttta aatggttact ggcaggatga caactaagga
    12781 tctctcaaaa accattttag agtagaatta tgtaacctga agagagacaa cccctaaacc
    12841 ccttattaag aaaaataagt tagcttatac ccaaactgag aaacttctct ctttttgacc
    12901 cttttaaccc ctagaaccct tgataaattt acattctatt tctctgtttc ttgtcttttt
    12961 cttattttgt agtgcgttgc tttgaggtat tttatgtttc taaacatcct cacaagggat
    13021 tgatgtcacc gccggcgtat aattgaccta ggaaagccgg aataaatatt acccacccct
    13081 taagctataa tccctctaat aggattcaaa agattggagg gaaatacatg ctaaggagtg
    13141 agtcaattat gatgttacac caaatgagaa aagaagggaa gagtatcaga ggtataagta
    13201 gagagatagg gctttctcgt aatgggtctt ggggatacta taattaccct cctcgattcc
    13261 tgcaaaatct aaaaacactc ttttccaaat accacagaac aatggaaata agtatcactc
    13321 cagcccccac cattgcccac tttcctggca cctctcccat aaacaggaat acccacaagg
    13381 ggttaagaat tggttcaata atgggaataa ggctggcttc caaggcggtt actcccttta
    13441 tagctataga atagagaata taagatatac ccagctgaac tgttcccaga aaaataagag
    13501 ttgcccagct ccccggggga ggagctccct gccacataaa aggaaggcca attaaagcgg
    13561 taaaaatatt tcctaaaagc actgattcta aaggagaaga atccttttgc attctcataa
    13621 aaataattaa aaaagcaaag gtccagccgc ttaaaacagc taataggttc cccacaaggt
    13681 ttccccactg tagttgatcg aaaaagaaaa gaaccattcc ccccagaact gcccccactg
    13741 cccaccaatc ctctcgagca acctcttctc ctaaaaacca ttttcccaga aaagccacat
    13801 acaccggagc accatactga agaagaatag cattagcagc tgtcgtcagt ttattagcag
    13861 caaca
//
LOCUS       DAOPSS010000032        12896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4086_length_12896_cov_9.772214, whole genome shotgun sequence.
ACCESSION   DAOPSS010000032 DAOPSS010000000
VERSION     DAOPSS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_12896_cov_9.772214"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKN31_03290"
     CDS             <1..238
                     /locus_tag="PKN31_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA98987.1"
                     /translation="CREKKQEFISCFFCWWDRICMIFLSFNRSSIFFRKNSSLIVEHL
                     LSLWDRSIIVEIFVVNPYYILIAFLKTLLYYGKM"
     gene            266..1522
                     /locus_tag="PKN31_03295"
     CDS             266..1522
                     /locus_tag="PKN31_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOA98988.1"
                     /translation="MGKTMAEKILENKAGFPVKAGDLVIAPVDRAMGQDGTTPLAIQS
                     FEEMKGERVFDPEKIILILDHNAPSPLESVSRLHDLMRNFSRKFGVQLMEIGEGVCHE
                     LMVSRGFVVPGDLVVGADSHTCTYGAIGAFSTGVGSTELAVVMLSGELWFKVPETIRL
                     NLQGKLPAGVFSKDVILYLAGKLGADGATYQALEFHGPVIENLEVEERFTISNMAVEV
                     GAKAGLMVPDEKAIKWVRERTNREFQPVFPDPDAQYSRVIDIDCSSLSPQVALPHQVD
                     NVKPIEEVGEVPIQEAFLGTCTNGRLRDIEVAARILEGRKIHPEVRFIVAPASREIFL
                     EAIKRGWVETIVLAGGVFVTPGCGPCVGTHQGVPGDGWNVISTANRNFKGRMGNAQSF
                     IYLASPATVAASALEGKITDPRKYLR"
     gene            1529..2035
                     /locus_tag="PKN31_03300"
     CDS             1529..2035
                     /locus_tag="PKN31_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOA98989.1"
                     /translation="MILRGRAHKFGDNINTDYIISGKYKFKTLDMNELAKHVMEDIDP
                     HFYEKVKPGDFIVAGKNFGCGSSREQAPRALSAAGIRGILAQSFARIFFRNAINCGLA
                     ALECDTSLIEDGDELEIDLLTGKIVDHSQGIEIKAQPLPPVMVDILREGGLVEYFKKY
                     GSFQGVRK"
     gene            2039..3295
                     /gene="glyA"
                     /locus_tag="PKN31_03305"
     CDS             2039..3295
                     /gene="glyA"
                     /locus_tag="PKN31_03305"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOA98990.1"
                     /translation="MWELVKELDPEVYYWIIKELERERNKLELIASENFTSLAVMQAQ
                     SSLLTNKYAEGYPNRRYYGGCEFVDEVENITIERAKALFGAEHANVQPHSGSQANMAV
                     YFAILKPQDTVMGMDLSHGGHLTHGSRVNFSGMLYNFVPYGVDPSTETIDYVALEKKA
                     KEVKPRLIVGGASAYPRFIDFERMRHICDEVGAYFMVDMAHIAGLVAAGVHPSPVPYA
                     HFVTTTTHKTLRGPRGGMIFCQKEFASAIDRAIFPGIQGGPLLHVIAAKAVALREAMT
                     REFVDYQKQVVKNAKVLGESLRSRGYRLVSGGTDNHLLLVDLRKAGITGKEAEKILDE
                     VGITVNKNTIPFDEEKPAVTSGIRIGTPACTTRGMKEREMEIIAECMDKALRGRDDSR
                     ILNQVKSQVRELCQKFPLYPEITNGE"
     gene            3347..3568
                     /locus_tag="PKN31_03310"
     CDS             3347..3568
                     /locus_tag="PKN31_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOA98991.1"
                     /translation="MREKVEKALDKIRGYLQFEGGDVELVDIEDGVVKVRLKGACSAC
                     PMAMFTIKDGIEQVIKEEVPEVKSVEQVL"
     gene            3926..4684
                     /gene="deoC"
                     /locus_tag="PKN31_03315"
     CDS             3926..4684
                     /gene="deoC"
                     /locus_tag="PKN31_03315"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOA98992.1"
                     /translation="MEEIEEWKKKVEDHLGVKIKEKREFPPEDIPREEEALSQFLARH
                     IDHTLLKPHTTLGEVENLCQEAKEWNFWGASVNPFYIPACAQLLRGTKTVVVAVAGFP
                     LGQNKPEIKAREARLAFQEGAREVDMVINLGALKNGDWKLVYQDIRGVVEEVSPGIVK
                     VILENCYLSPEEKVVGCLIAQAAGAHFVKTSTGFGSGGATVEDVELMRQVVGESMGIK
                     AAGGIRSREVALQMIKAGANRLGTSSGVKIMKGE"
     gene            complement(4668..5360)
                     /locus_tag="PKN31_03320"
     CDS             complement(4668..5360)
                     /locus_tag="PKN31_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOA98993.1"
                     /translation="MFRGWQKVSYIEYPGNIATVLFTGGCNFRCPYCHNPELVENYSI
                     FPEIRAEEVLQYLEERKGLIDAVCITGGEPLLWKEELLPFLIKIKEQGFKVKVDTNGS
                     SYSSFLSFEEIVDLWGIDYKVPLSSYHLVQGEKEIQSVREVLREALKFPEKVEFRTTI
                     FPPFHQKETLREMAEEIKSAHTWYWQNFNPFKTLDKEAQKVTPYSFSLLQKWQDEVNR
                     EVGKDLVVIRPS"
     gene            complement(5363..7447)
                     /locus_tag="PKN31_03325"
     CDS             complement(5363..7447)
                     /locus_tag="PKN31_03325"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOA98994.1"
                     /translation="MKEKIDLSPKEVRKRDGRVVPFDRQRIERAIYKAFQAVGEENTT
                     IPEELSHIVTQKLFHKFGTDTTVDIEVIQDIVEETLIENGFAKVAKAYILYRRKRQEA
                     REALQVAVNVEKIVQDYLIQADWRTQENSNTTYSYPGLVLHVAGSVMAHYTLNQIYPD
                     EVKEAHVNGDIHIHDLSYGITAYCAGWSLEELIREGFGGVKDKIAAGPARHLSALTGQ
                     MVNFLGTMQMEFAGAQAFNSVDTYLAPFVRYDQLDYRATKQLIQQMVFAMNVPSRWGS
                     QAPFLNFSFDWVAPEDLKDRPVLIGGEERPDLGTYGEYQEEMDMINRAFIEVMLEGDY
                     EGRVFSFPIPTYNITPDFPWDSENAQLLWELTAKYGVPYFQNFISSSLKPGDVRSMCC
                     RLQLDLRTLRNRCGGLFGSADKTGSIGVVTINLPRLAFLSKTEEEFFTRLDKLMNIAK
                     TALEIKRKVVERNLRNGLLPYTRRYLGTFRNHFSTIGLLGMNEACLNFLGVSIAEKEG
                     KDFAVKVLDFMRNKLADFQEETGNLYNLEATPAEGASYRLARCDRRDFPDIISAGEEE
                     PFYTNSTNLPVNYTDDPFEALEHQKDLQTLYTGGTVFHAFLPEMPHPEAVKLFIKRTF
                     EHYPIPYLTVTPTFSVCPNHGYLRGKKFICPECEAETEVYSRVVGYYRPVQRWNKGKQ
                     EEFKERKEFAIP"
     gene            complement(7431..7925)
                     /gene="nrdR"
                     /locus_tag="PKN31_03330"
     CDS             complement(7431..7925)
                     /gene="nrdR"
                     /locus_tag="PKN31_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOA98995.1"
                     /translation="MHCPFCEEEDSRVVDSRIVEGGAAVRRRRECPRCQRRFTTYERY
                     EQQPLRVIKKSGERELFNRQKILQGMLKACEKRPVTMEVLESLAGEIEKELRDEGYEE
                     IPTSHIGEKVMEKLKEVDQVAYVRFASVYREFREIDQFLELLSTLNREKNTSERREVD
                     EGKD"
     gene            8429..11266
                     /locus_tag="PKN31_03335"
     CDS             8429..11266
                     /locus_tag="PKN31_03335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOA98996.1"
                     /translation="MRTSDFQGKVFPVTGGVKIVKKMELTREEILTEIINKPLPLAEE
                     QAKVVTSEAKHIRVIAGAGTGKTETLTRRLLYLLLYEGVPPESIVAFTFTEKAAASMK
                     TRIYSRIRELTGENFPELSGMYIGTIHAYCLHLLHNYFGYSEFNVLDENQEVALLFRV
                     GHELGIRENVQGRNYLQKCLNFTQAVNIVYNELIDRKRLSEKCDIFHSLLCDYEELLD
                     RYRLLTFGRMIDLAIQNLKKESNRVSYIQYLMVDEYQDINRAQEELIRLLGREASVFV
                     VGDPRQSIYQWRGSDERFFEEFEKCFPGVQTFYLMENRRSVPPIVRAANYFENTFKKG
                     YGPISAKRKDQGFVGLVSLPDPEEEARWVVKEIASAVRSGKCRFKDCAILLRSVANYS
                     VPFINELRRRDIPYLVGGNVGLFFREEAQAMGCLFAWLGKDGFWQAGESEIRGDLLVQ
                     KGVALWSKATGIALDREKIYSELREWREEVLQGKYQNLIQVFHRLLVILGYLKLDPSH
                     SLHAVLMANLGRFSSILFDYESSYRLGGAGVEWNEVVRGLCWYMNAYARGAYEEHSIE
                     DYLTTDAVFISTVHQAKGLEWPVVFVSSVVNARFPSMRAGKEKDWLLPRNLFDAKRYE
                     GSIEEERKLFYVALTRAKNVLCVTYFRTNRSKKGNIVSRRLSVFVEELSEVLPVHTDD
                     AYITFGEIDKSEGEVFQALSLPQVVAYFRCPHFYRLRELWQYRPGLAEELDYGRSLYN
                     CLRMSVELIKAGEKPHRAVERAMTEKFFLPFAGEKKSSAMKQKASATLLEFVENNLDE
                     LMKIDKMKAQIELPTDNAVLAGSIDAILEDSEDSAVWMCRTSQEVVTPEEIEFQLGLY
                     ALFIKLAQGYIPRTFFVDLGNATVEEFPADEEKVEVVAEKLKDILTGIRSGDFEVSQK
                     KEHCAKCDYSGICRFCDGGSSPPSPKKRVRR"
     gene            11494..12525
                     /locus_tag="PKN31_03340"
     CDS             11494..12525
                     /locus_tag="PKN31_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOA98997.1"
                     /translation="MREAESLREDKPTRYRSTFSRRSLLFRLYRVVQEIQKDTYPGVS
                     QLAEILEVSKRTVNRYIEMLRDDFDAPIVYDRGRGGYYFEESWSMPFPNLTEGETLAL
                     LIFLDVIEQFKGTPLEEAFNRLGEKLKLALPDSLKISYEDFEMLLSPFLVPLTMRVDV
                     SKVFGELFRAITDRKRALISYYSFSSRQVTERKVDPYHIYNFEGVWYFCGFCHLREEI
                     RDFALDRVQEVKVLEESFARPPDFSPQEYLNRAFRMYRGEATQIRIRFDSYQSAWIKE
                     RIWHPAQEIEELPDGGIIFTIYGHPEEIKRWVIGYGAHAEILEPQYLRKEIAEEIEKL
                     QRIYHNDRK"
BASE COUNT         3568 a         2533 c         3184 g         3611 t
ORIGIN      
        1 ctgccgggag aaaaagcagg agtttatctc ctgctttttt tgttggtggg atagaatctg
       61 catgattttc ttatctttca ataggagctc tatttttttt agaaagaact cttccctgat
      121 agtagaacat ctcttatctt tatgggatag aagtatcatc gtagaaattt ttgtagttaa
      181 cccctattat attctcattg cttttttaaa aaccttgctg tattatggga aaatgtaaaa
      241 aggagcttag ataaggagcg atactatggg aaaaaccatg gcagaaaaga tactggaaaa
      301 caaagctggt tttccggtta aagcaggaga cctggttata gctccggtag atagagctat
      361 ggggcaagat ggtactactc ccttagctat ccaatctttt gaagagatga agggggagcg
      421 agtttttgac ccggagaaga taattctgat tttagaccac aatgctccca gccctttaga
      481 atcggtttcc cggcttcatg acctgatgcg caacttttcc cggaagtttg gagtgcagct
      541 tatggaaata ggagaaggag tgtgccatga actgatggta tcccggggtt ttgtggtgcc
      601 gggagattta gtggtgggag ctgattccca tacctgcact tatggagcga ttggagcttt
      661 ttctaccggg gtgggcagta ctgaattagc agtggtaatg ctttctggag aattgtggtt
      721 taaagtgcca gaaactatcc gcttgaacct gcaaggtaaa ctaccagcgg gggtattttc
      781 taaagatgtt attctttatc ttgctggcaa attgggagct gatggagcca cttaccaggc
      841 tctggagttt cacggaccgg ttatagaaaa cttagaagta gaggaacgtt ttaccatttc
      901 taatatggca gtggaagtgg gagcaaaagc tggtcttatg gttcccgatg aaaaggctat
      961 aaagtgggtg agagaaagga ccaatcggga gtttcaacca gtttttcctg accctgatgc
     1021 gcaatattct cgggttatag atatagactg ttcttctctt tctcctcaag tggctcttcc
     1081 tcatcaggtg gataatgtaa aaccaataga ggaggtagga gaagttccca ttcaagaagc
     1141 ctttttaggg acctgcacca atggaagact gcgagatata gaagtggctg ctcgtattct
     1201 ggagggaagg aaaatccatc cagaagtgcg attcattgtt gctcctgctt ctcgagaaat
     1261 ctttttggaa gcgataaaga gaggatgggt agagacaatc gttcttgccg gaggagtttt
     1321 tgttaccccc ggatgtggcc cttgtgtggg aactcatcag ggagtcccgg gagatgggtg
     1381 gaatgttatt tctactgcta accgtaattt taaagggaga atgggcaatg ctcaatcttt
     1441 catttattta gcttctccag ccacagtggc ggcttcggct ttagagggta aaattaccga
     1501 tcccagaaaa tatctgaggt gagagaaaat gattttgcga ggtagagctc ataagttcgg
     1561 agataatatc aataccgatt atattatctc cgggaaatat aagtttaaaa ctttagacat
     1621 gaatgagttg gctaaacatg tgatggaaga cattgaccct catttttatg agaaagtaaa
     1681 gccaggcgat tttatagtgg cggggaaaaa ctttggttgt ggttcttccc gggagcaggc
     1741 tcctcgagcc ctctctgcag cgggaatccg ggggatcctc gctcagtctt ttgcccgtat
     1801 tttctttcgc aatgccataa attgtggtct ggcagcttta gaatgtgata cctctctaat
     1861 agaagatgga gatgaactgg aaattgacct tcttaccgga aaaatagtag accactctca
     1921 gggaatagag attaaagctc agcctctgcc tccggttatg gttgatattt tgagggaggg
     1981 gggactggtg gaatatttca aaaaatacgg gtcttttcag ggggtcagaa aataagccat
     2041 gtgggagtta gtaaaagaat tagacccgga ggtttattac tggataataa aagagttaga
     2101 gcgagagaga aataaattag aattaattgc ctcagaaaat tttaccagtt tagctgtgat
     2161 gcaagcgcag agctctcttt taaccaataa atatgctgaa ggatatccta atcggcgcta
     2221 ttacgggggt tgcgagtttg tagatgaggt ggaaaatatc accatagaga gagctaaagc
     2281 tctttttggg gcagagcatg ccaatgttca gccccattct ggctctcaag ctaatatggc
     2341 ggtttatttc gccattctta agcctcagga tacagtgatg gggatggatt tatctcacgg
     2401 aggacatctc acccacggaa gccgggtcaa tttttcagga atgttataca attttgtgcc
     2461 ctatggagta gacccctcca cggaaaccat agactatgtg gccctggaga aaaaagcaaa
     2521 agaagttaag cctcgcttga tagtgggagg agccagtgct taccctcgct tcattgattt
     2581 tgaaaggatg cgccatattt gtgatgaggt aggcgcttat tttatggtgg acatggccca
     2641 cattgccggg ttagtggcag cgggagttca tcccagtcct gtcccttacg ctcattttgt
     2701 taccaccacc actcataaga ccctccgggg tcctcgggga ggaatgattt tttgtcagaa
     2761 agaatttgcc tcagctattg accgggctat attccccgga atccagggtg ggcctctcct
     2821 ccacgttatt gccgcaaaag cagtagcgct ccgggaggca atgactcgcg agtttgtgga
     2881 ttatcaaaaa caggtagtta agaatgctaa agttttagga gagagcctcc gctcgcgggg
     2941 gtatcgtctg gtctcgggag ggactgataa tcatctgctt ttagtggatt tgcggaaggc
     3001 agggataacg ggaaaagaag cagaaaaaat tctggatgaa gtggggataa cggttaataa
     3061 aaatactatt ccttttgatg aggaaaaacc tgcggtaaca agtggcatca gaattggtac
     3121 ccctgcttgc accactcgag gtatgaaaga gagggaaatg gaaatcattg ctgaatgtat
     3181 ggataaggcc ctgcgaggaa gagatgactc ccggattctc aaccaggtca aaagccaggt
     3241 gagggaactg tgccagaaat ttcctctgta tcccgaaata accaacgggg aataaaatat
     3301 aaaaaggtgg tttacttatg gagaaactat actatggagg tagaaaatga gagaaaaagt
     3361 tgaaaaagct ctggataaga taaggggtta tctccaattt gagggcggag atgtggaact
     3421 ggtggatata gaggatggag tggtaaaagt cagactgaaa ggtgcctgta gcgcttgccc
     3481 tatggctatg tttaccatta aagatggtat tgaacaggtg ataaaagagg aagtgccgga
     3541 ggtaaagtcg gtagagcaag tgttatagag ctataaaagg gacacctcta ttgttttgaa
     3601 acattcagga ataacaacac gggggaagag cctccgtgtt gttattggct tcttctcttg
     3661 tttttgaggg ctaatagggg agatttgtct cccttatagt tctctcgtca ttcccgaaat
     3721 ttaggcggga atccaaaggc ttttaaatac tgtggacccc tcgcagaacc attcaggggt
     3781 gacgacaaaa aaagacacct tcatccgata aagctctatt ttagattgag ggttttacaa
     3841 aaaaggaaag aaggaataga atagacaatc agtagaagaa aaattgctgc agtaaaataa
     3901 ttaaaagagg aggtagaaaa tgaggataga agaaattgaa gagtggaaaa aaaaggtaga
     3961 agaccactta ggagtgaaga ttaaggaaaa gagagaattt ccccctgaag atatcccccg
     4021 ggaagaagag gctctatcgc aatttttagc ccgccacatt gaccacacgc tgctcaaacc
     4081 ccatactact ttaggagaag tagaaaatct gtgtcaggaa gctaaagagt ggaatttttg
     4141 gggagcatcc gttaaccctt tttatattcc tgcatgtgcc cagcttctca gaggcacgaa
     4201 gacagtagtg gtggcggtgg ctggctttcc actgggacag aacaaaccag agattaaagc
     4261 ccgagaggct cgcttagcct tccaggaggg agccagggag gtagatatgg taataaacct
     4321 gggagccctc aaaaatggtg attggaagtt agtttaccag gacatccggg gagtggtgga
     4381 ggaagtgtct ccagggatag tgaaagtgat tcttgagaat tgttatctat cccccgaaga
     4441 aaaagtggtg ggatgcttga ttgctcaagc tgcaggagct cattttgtaa aaacttccac
     4501 cggctttggc tctggtggtg caacagttga agacgtagaa cttatgcgtc aggtagttgg
     4561 agaaagtatg gggataaagg cagcaggcgg tatccgctcc cgggaagtag ctctgcagat
     4621 gattaaggct ggagccaacc gtttaggtac ttcaagcgga gtgaaaatca tgaagggcga
     4681 ataaccacca ggtcttttcc tacttcacgg ttaacctcat cttgccattt ctgtagtaga
     4741 gaaaaggaat aaggggtgac cttttgagct tctttatcta aagttttaaa aggattaaag
     4801 ttttgccagt accaggtatg agcgcttttt atttcttctg ccatttcccg cagagtttcc
     4861 ttctggtgaa aaggagggaa aatggtggtt ctaaattcta ctttctccgg gaattttaaa
     4921 gcttctcgca atacctctct aacagattgt atttcttttt ctccttgcac gagatggtaa
     4981 gaagagaggg gcactttgta atcgatgccc cacagatcta ctatttcttc aaaagacaga
     5041 aaggaagaat agctgctccc gttagtatcc actttgacct taaatccttg ctctttgatt
     5101 ttaataagga aagggaggag ctcctccttc cacagaagtg gctctcctcc cgtaatgcat
     5161 actgcatcta tcaagccctt tctctcttcc aaatactgta acacttcttc tgctcttatt
     5221 tccggaaata tagaataatt ttccaccagc tccggattat gacagtaagg gcaacgaaaa
     5281 ttacaccccc cagtgaaaag cactgtagca atattgcctg ggtattctat ataacttact
     5341 ttttgccagc ctcgaaacat ttttaaggga tagcgaattc tttacgctct ttgaattctt
     5401 cttgctttcc tttattccat ctctgaaccg gtcgataata gcctactact cgagagtaaa
     5461 cttctgtttc tgcttcacac tctggacaga tgaacttttt acctctcaga tatccatgat
     5521 tcggacaaac tgaaaaagta ggagtgacag tgagataagg aataggataa tgctcaaaag
     5581 tccttttgat aaatagcttt accgcttcag gatgaggcat ttcagggagg aaagcgtgaa
     5641 ataccgttcc tccggtatat aaagtttgca aatctttctg gtgttccaga gcttcaaatg
     5701 ggtcatcggt gtaattgacc ggcaaattag tactattggt ataaaaaggc tcctcttctc
     5761 cggcacttat gatgtcgggg aaatctcggc gatcacatcg agccaggcgg tagctggctc
     5821 cttctgccgg ggtggcttct aaattataaa ggttaccagt ttcttcttga aaatcggcta
     5881 atttatttct catgaaatcc aataccttga cggcaaaatc ttttccttct ttttcagcga
     5941 tggatactcc taagaagttg agacaggctt cattcatccc cagcaggcct atggtggaga
     6001 aatgattgcg gaaagttcct aaatagcgac gagtgtaggg caggagcccg tttctcaggt
     6061 ttctttccac cacttttctt ttgatttcta aagcagtttt agcaatattc attaatttat
     6121 ccaggcgagt aaagaattcc tcttcagttt tactcaagaa agcaagacga ggaaggttga
     6181 tggttacgac accaatagag cctgttttat cggcagaacc aaaaagccct ccacagcggt
     6241 tgcgcaaagt ccggagatcc agttgcagcc ggcaacacat agagcgcaca tctccaggct
     6301 ttaaggaact ggagataaag ttttggaaat acgggactcc atatttagcg gtaagctccc
     6361 agagcaattg cgcattttct gaatcccagg gaaaatcggg ggtgatatta taagtaggaa
     6421 tgggaaaaga aaacactctt ccttcgtaat ctccctctaa cattacctcg ataaaagccc
     6481 ggttgatcat atccatttct tcctgatact cgccataagt tcccaggtct ggcctttctt
     6541 ctccccctat taggaccgga cggtctttta aatcttcggg ggctacccaa tcaaaagaaa
     6601 aattcaggaa gggagcctgg cttccccatc gggaggggac attcatggca aacaccatct
     6661 gctgaataag ctgtttggta gcccggtaat cgagctggtc ataacgcacg aaaggagcga
     6721 gatatgtatc tacagaattg aaagcctgag caccggcaaa ctccatttgc atggtgccta
     6781 aaaaattaac catctgtccc gtgagagcag aaagatggcg ggccgggcct gcggctattt
     6841 tatcctttac ccctccgaaa ccctctctga taagttcttc cagagaccat cctgcgcaat
     6901 aagcagtaat tccataggat aggtcgtgaa tgtgtatatc accattaaca tgggcttctt
     6961 tcacctcgtc gggatatatc tggttcaaag tatagtgagc cataaccgag ccggcgacgt
     7021 ggagaaccaa ccccggataa gaataagtag tgttagaatt ttcttgtgtt ctccagtctg
     7081 cctggataag ataatcctga acaatttttt ccacgtttac tgctacttga agagcttctc
     7141 ttgcttcttg ccttttgcgc cgataaagaa tgtaggcctt ggctactttg gcaaatccat
     7201 tttcaataag ggtttcttcc actatatcct ggatgacctc aatgtctaca gtggtgtcag
     7261 taccaaactt atggaagagc ttctgagtaa ctatatgact tagctcttcg gggatagtgg
     7321 tattttcctc tcctacagcc tggaaagctt tataaatggc tcgctcaatc ctttgccgat
     7381 cgaaagggac taccctccca tcccttttcc gtacttcttt gggggataaa tcaatctttt
     7441 ccttcatcaa cctctctcct ttccgaggtg tttttttctc gattcaaagt agaaagcagt
     7501 tctaagaact ggtcaatctc tcgaaactcc cggtaaaccg aggcgaagcg aacataagcc
     7561 acctggtcta cttcttttag tttctccatc actttttcac caatgtgaga agtggggatt
     7621 tcttcataac cctcatcccg cagctccttt tctatttctc cagccaggga ctctaaaact
     7681 tccatagtta ccggtctttt ttcacacgct ttgagcattc cttgcaaaat tttttgccgg
     7741 ttgaacagct ctctctcccc actttttttg attactcgaa gaggttgctg ttcataacgc
     7801 tcataggtgg tgaatctgcg ctggcatcgg gggcattctc tcctccggcg cactgccgcc
     7861 ccaccttcta ctattcgaga gtcaacaact cgggaatctt cttcctcaca aaaaggacaa
     7921 tgcataataa tttacccaac atattgaatt attgtttcta acaaccacta catatagtat
     7981 acaggttttt attttaattg caagaggaaa attacgaatt tagaaaaata ttttttcttg
     8041 tggaaaactg ggaaggctaa tttatagtta aagctgtaaa aacaattatg atgtcctcta
     8101 tttggtgttc tgcttctttt ttctctcagg gctggggcat ttttccctca atgcattttt
     8161 atgctttttg agttttttgg tctgttttct tcaattttgt gcttcaggct gttctatttt
     8221 ttctcttacc cgtggtatcg gttctaactt ttttgttgtg attttttgtg ggggtcatag
     8281 gagctcttgc cttctttttg tttttagcgc tgcaggctgt ttgtggcgtc tcaagctttt
     8341 agcacgatgt aactcgcctt taaggtttga gcaatttgac aggttttgtg tgatactata
     8401 atagggtagt ttttgctcaa gctatacgat gagaacaagc gattttcagg gaaaagtttt
     8461 cccggttact ggaggggtaa aaatcgttaa gaagatggag ctcaccaggg aagaaatcct
     8521 taccgaaata attaataagc cccttcccct ggccgaagag caagcaaaag tagttacctc
     8581 ggaggcaaaa catatcaggg tgatagcggg ggcgggtacg gggaagactg aaactctcac
     8641 ccggagattg ctctaccttc tgttgtatga aggtgtgcct ccagaaagta ttgttgcttt
     8701 taccttcact gaaaaagctg ctgcaagcat gaaaaccagg atatattccc gtattcgcga
     8761 gctgaccgga gagaattttc cggagttaag cggtatgtat attggcacca tccacgccta
     8821 ttgtcttcat cttttgcata attactttgg ttattcggag tttaatgtgc ttgatgaaaa
     8881 tcaggaagtt gctttgttat tcagagtggg ccatgaactg ggtatcagag aaaacgtgca
     8941 ggggaggaac tacctgcaaa aatgtctcaa ttttacccag gcggtaaaca tagtttacaa
     9001 tgagctgata gacagaaagc gactctcaga aaagtgcgat atctttcatt ctcttctctg
     9061 cgattatgaa gaacttctgg accgctatcg tttgctcact tttggaagaa tgatagacct
     9121 ggcaattcag aatcttaaaa aagaaagtaa tcgtgtaagc tacatccagt atttgatggt
     9181 ggatgaatat caggatatca accgggctca agaggagctt atcaggctcc tggggcgaga
     9241 agcaagtgtg tttgtagttg gtgacccgcg gcagagcata tatcaatggc gggggtcaga
     9301 cgagcggttc tttgaagaat tcgaaaaatg cttccccggg gtgcagacct tttatctgat
     9361 ggagaatcgc agaagcgtac ctcccatcgt tagagctgct aattactttg agaatacctt
     9421 caaaaaaggc tatgggccta tatctgctaa aaggaaagac cagggttttg taggattggt
     9481 atctcttccg gatccggaag aggaggccag atgggtagtg aaggaaattg cctccgccgt
     9541 tcgaagcgga aaatgccgtt tcaaagactg tgccattctt ctgcggagtg tggctaacta
     9601 ttctgtacct tttataaatg agcttcgcag gcgcgatata ccgtatctcg tgggaggtaa
     9661 cgtaggactg tttttccgag aagaagctca agctatgggt tgtctctttg cctggctggg
     9721 aaaggatggt ttctggcagg cgggcgaaag cgagataagg ggtgatttat tggtacagaa
     9781 aggagtagcg ctctggtcta aagctactgg cattgccctg gatagggaaa agatttattc
     9841 ggagctgaga gagtggaggg aagaagtgct acagggaaaa tatcaaaatt taatccaggt
     9901 ctttcaccgg cttttagtaa ttcttggcta tctaaagctc gatccttctc actccttgca
     9961 tgcggtgttg atggccaacc tgggtcgttt tagctccata ttgtttgatt atgagtcttc
    10021 ctaccggctg ggaggagcag gagtagaatg gaacgaggta gtgaggggct tgtgctggta
    10081 tatgaacgcc tatgctcgcg gagcttatga agaacactct attgaggatt atctgaccac
    10141 tgatgcagta tttataagca ctgtgcacca ggccaagggg ctggagtggc cggtggtttt
    10201 tgtatcttcg gtggttaatg cccgatttcc ctccatgaga gcagggaaag aaaaagactg
    10261 gctgcttccc agaaacctct ttgacgccaa gcgctacgaa ggaagtatag aagaagagcg
    10321 aaagctattt tatgtagcac tgaccagggc aaaaaacgta ttatgtgtta cttattttag
    10381 aaccaaccgg agtaaaaagg gaaacatagt ttctcgccgt ctcagcgtgt ttgtggaaga
    10441 gttgagtgaa gtcctgccgg tacacacaga tgatgcatat ataaccttcg gagaaataga
    10501 taagagcgag ggagaagttt ttcaagcact ttctctcccc caggtcgttg cttattttcg
    10561 ctgtcctcat ttttaccggt tgcgggagct atggcaatac cgtcccgggc ttgcagagga
    10621 gctggactat ggaaggagtc tttataactg cttgaggatg tcggtagagc ttataaaggc
    10681 aggggaaaaa cctcaccggg cagtggagcg agctatgaca gaaaagttct ttttaccttt
    10741 tgccggggag aaaaagagtt ccgctatgaa acaaaaagcc agtgcaaccc tactggagtt
    10801 tgttgaaaat aatcttgatg aattaatgaa aatagacaaa atgaaggccc agattgagct
    10861 tcctaccgat aatgcggtgc tcgccggtag cattgatgcc atactggaag atagtgaaga
    10921 ttcagcggtg tggatgtgtc gaacttctca ggaagtggta actcccgaag agatagagtt
    10981 tcagctggga ctctatgccc tgtttattaa gttagctcaa ggttacatac ctcgaacttt
    11041 ttttgtggac ctgggcaatg ccacagtgga ggagtttccc gcagacgagg aaaaagtaga
    11101 ggtggtagcg gagaagctga aagatatcct gactggaatc cgcagcggtg attttgaggt
    11161 aagccaaaag aaggaacact gtgcaaaatg cgactactct ggaatatgcc ggttttgtga
    11221 tggaggcagt tccccacctt cgccaaagaa aagggtgagg cggtgaggga atttcatttt
    11281 attaacgggg gagtttccat cattagcaat ctctgtacgg atgggacacg agatgaagat
    11341 actgtctctc ttttcttagg gtataataag ttagtgccgg ctcatgttga gccagaatat
    11401 ttaggctaaa ggaaagaact ccaggaagat attgctcatc gggttagctg ataaacaact
    11461 ggccgggttt aagatacagg aggtggggaa aagatacgag aagcagaatc tctcagagag
    11521 gataaaccta cccgctatcg gagcactttt tccaggcgtt cactactttt ccggctttac
    11581 cgcgttgttc aagaaatcca aaaagatacc taccccggtg tttcccagct tgcagagatc
    11641 ctcgaagtta gtaagcgcac tgttaaccgt tatattgaaa tgttaagaga tgattttgat
    11701 gccccaattg tctacgaccg agggcgtgga ggttattatt ttgaggaatc ctggtcaatg
    11761 ccctttccta accttaccga aggagagaca ctggctcttt tgattttcct cgacgttatc
    11821 gaacagttca aaggtacgcc cctcgaggaa gcttttaatc gtctggggga gaagctaaaa
    11881 ctggcgctac cagatagcct caaaataagc tatgaagatt tcgagatgtt gctctctcct
    11941 tttcttgtgc ctcttactat gcgggtggat gtaagcaagg tttttggtga gctttttaga
    12001 gctataactg accggaagag agctttaatt agctattata gtttttcctc tcgccaggtt
    12061 accgagcgca aggtagaccc ctatcatatt tataatttcg aaggggtgtg gtatttttgt
    12121 ggcttttgcc acttgcgaga agaaattcgt gactttgctc tggaccgggt gcaggaggta
    12181 aaagtactgg aagaaagctt tgcacgacct ccagattttt cgccgcagga gtacttaaac
    12241 cgtgcttttc ggatgtatcg gggggaagct actcaaatac ggattcgttt tgacagttat
    12301 caatctgcct ggataaaaga gcgtatctgg catcctgccc aggaaataga agagcttccc
    12361 gatgggggaa ttattttcac catttatggc catcctgaag aaattaagcg ctgggtgata
    12421 ggttacggcg ctcatgccga aattttagaa cctcaatatc tgcggaaaga aattgcggaa
    12481 gaaattgaaa aattgcaaag aatttaccac aacgacagga aatgacacca tcactgagta
    12541 tgatgagata gaaggtaaaa aaatctttca tcattgaaat taattttggg ggagtggaat
    12601 gatggataac gaaacaagag ctaaagatag agtttgtcct cagtgcaaag gcactggccg
    12661 ttgcaaatac tgtagtggtt cgggaaaaca tgcttaccct ggatatggaa agccgagcga
    12721 ccagccttgt atgtggtgtt ttggtaccgg ggtttgccag cagtgtaaag gtaagggtca
    12781 ttattaaatc atttttagaa ggagggaaac ctgtagagct ctgagagttt ataaaaagcc
    12841 tggctgatgt ttgaactatg cccgggttca gatgccggct cagtaaagaa aggagg
//
LOCUS       DAOPSS010000033        12794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4125_length_12794_cov_8.225528, whole genome shotgun sequence.
ACCESSION   DAOPSS010000033 DAOPSS010000000
VERSION     DAOPSS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4125_length_12794_cov_8.225528"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1188)
                     /locus_tag="PKN31_03345"
     CDS             complement(226..1188)
                     /locus_tag="PKN31_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA98998.1"
                     /translation="MAEKGGRRAFAERNLLVIIILMAIFLSIATKGNFYSWENISNLL
                     RQSSINGVVAIGMTLIIITGGIDLSVGSIVGLSGMTYAILTSNRGEIQMSSGLAILIS
                     LGIAALIGLANGVAVHDGKVPPFIATLGMMTLVRGLVMYVSSGRMITGIPMGFRQFSV
                     ATFLNIPALAWIWIILALFMAFVLKYTMFGRNLYAIGSNKEAARLSGINIRLNMYSFY
                     TVAALFSGIAGLMLATRMAAGVPTGGQGYELDAIASVVIGGASLSGGVGTVFGTALGA
                     LIIQTMRNGGNLLGVDPFIMQIIIGALIILAVFFDQYLKGRREE"
     gene            complement(1190..2713)
                     /locus_tag="PKN31_03350"
     CDS             complement(1190..2713)
                     /locus_tag="PKN31_03350"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA98999.1"
                     /translation="MSSKKEVLKLENICKSFPAVKALDQVSMSVSAGEVRGLVGENGA
                     GKSTLIKIVTGAYDKDSGKIIFEDNEIVRNSPIVSKKLGIYAVYQDVMIAPDLSVAEN
                     FFMGNQPKKGLIIDWGKMYEESEEFLRDMELEIDVKRNIRELSVAEGRMVTIAKCLWQ
                     EPKLVIFDEPTAVLTQNETQILFRIIRDLKKRGVAVIYISHNLEEVFDVCDTVTVLKD
                     GKLVGTYTVSELESVDKLIPLMVGRSIEEMYYKKEVEIGPELLRVENLSGKRFRNIEI
                     NVRAGEILGVFGLVGSGRTEIARAIYGADRFDEGKIVVNGQEILVKRPKDSLNSGLGY
                     LPEDRKNQGIFSQQDVEFNINVINLDKVMRAGIIDYSKAYQQAQDYVDKLSIKVGSVR
                     QKLSELSGGNQQKVVIARWLCKNPDILIFDEPTTGIDVGTKAEIYKLFGEILDQGKGI
                     ILISSYLPELIGLSDRIIVISNGEIAGEVEKEDFSEEYLLKLAMKNILNREKVQEAV"
     gene            complement(2787..3767)
                     /locus_tag="PKN31_03355"
     CDS             complement(2787..3767)
                     /locus_tag="PKN31_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99000.1"
                     /translation="MRKRLIPLILAFVLLGVTFAYAADVEPFDIAFIVKATDSDFWQY
                     TIVGAKNAEYDLKGLINITVYGPPSEADIDEQVAIVENVVNTKPDAIVIASTSSDATS
                     LVLNNAYEQGIKIILIDNFVHDTGYHSFLATNNKVGGALAADKLVEAIQAAGKPLEGK
                     IAVISAMAGVQVLIDRTEGFLERLEEIAPGLEVLPVRYADNDIAVAANIAEDILTANP
                     DLLGFFANNNHTGDGVARVIEERGLQDQIVAVAYDSDPQEIDALRSGALKALIVQDPH
                     GMGYKGVMFAFMSINGEPLPEYFDTGVYVITQENLDESMWILDPFSRKKY"
     gene            4086..4502
                     /locus_tag="PKN31_03360"
     CDS             4086..4502
                     /locus_tag="PKN31_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOA99001.1"
                     /translation="MRSYRKELWFQTEKRREFINITPLVEECLRESGIQEGLLLCNAM
                     HITASVFINDDESGLHRDFERWLEKLAPEKPYDQYEHNVGEDNADAHLKRTIMGREVV
                     VAITDGRLDLGPWEQIFYGEFDGKRRKRVLVKIIGE"
     gene            complement(4858..5700)
                     /locus_tag="PKN31_03365"
     CDS             complement(4858..5700)
                     /locus_tag="PKN31_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOA99002.1"
                     /translation="MGKISLQDLLKKVDDLPALPSVVKRVMELTEDPNSTARDINEVI
                     NQDQSMTAKVLRLANSAFYGFPRRISSVTDATIMLGFQTIRSIVIAASVSKLLSQEVE
                     GYALLPGELWRHSQSAAMAARLIARRIKFSKAEVAYTAALLHDIGKVVLNSYIRETYE
                     EVMDKVEKDKVPFSQAEEEILGFNHALVGSKVAENWNLPPELVEAIAFHHSPELAKTD
                     PVLTSIAHVADFICVTMGIGIGADGLLYPLSPQAVNLLGLEEQDIYQIISQLSDLLVD
                     QDSF"
     gene            complement(5821..6849)
                     /gene="gyaR"
                     /locus_tag="PKN31_03370"
     CDS             complement(5821..6849)
                     /gene="gyaR"
                     /locus_tag="PKN31_03370"
                     /EC_number="1.1.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047964 - glyoxylate reductase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate reductase"
                     /protein_id="HOA99003.1"
                     /translation="MINIKLWSFSGEEGVKLDKKPRVLVTRRIPQEGLNILEESCQVE
                     VSDYDGVMPREILLQKVKGVDGILCLLNDDIDWEVMEAAGQNLRVISNYAVGYDNIDI
                     EEATKRKIMVTNTPGVLTEATADLAWALLMAVARRVVEGDRFVREGKFRGWEPLLLLG
                     DDVYGATLGIIGLGRIGQAMARRARGFDMRIIYFDYHQVKDIEKELGVTYHPFPELLA
                     QSDFVSLHVPLTRETYHLIGEKELKMMKKESYLINTSRGPVIDEKALVRALREGWIRG
                     AALDVFEQEPQIEPELLGLDEVVLTPHLGSASHATRTKMAVLAARNLVLALQGETPPH
                     LVNPDVVK"
     assembly_gap    7494..7593
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7741..8718)
                     /locus_tag="PKN31_03375"
     CDS             complement(7741..8718)
                     /locus_tag="PKN31_03375"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOA99004.1"
                     /translation="MRLKTTPLMKGAMIAVRDCMGVKKEEKVVVITDTGKIDIAKSFL
                     FALHALGVDATLSLMVPRSHHVEEPPSEIGVAMASVDVALLVTTTSLSHTYARMEATK
                     KGVRIASMPGITEDMLTVGAMTADYEKVSELSWKLTHLLEATDLVEIYTEKGTDVRLS
                     LKGRKPAIPPDDGLYRERGRWGNLPAGEAFVAPVEESVEGIVVVDGSMSPLGLLAEPI
                     KLTIQRGKVIKIEGGREAEELSRFLEELSDPYAYWVGELGIGTNEKARITGNILEDEK
                     ALRTVHIALGMNVSIGGKVESKTHNDGIIRNPTVKFDGELIMDKGILMV"
     gene            9033..10346
                     /locus_tag="PKN31_03380"
     CDS             9033..10346
                     /locus_tag="PKN31_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA99005.1"
                     /translation="MPVFRILIVDDEESLREMLKEFLEGEGMLVELAQNGREALEKLH
                     SLSFDLVLLDLRMPGVSGVEVLQEIKKEEPELPVVVITAYGSVDNAVETLKMGAFDFI
                     TKPFKLEELLNTISRALEVERLKREKQYLLEEMQDKFCFEGVVGQSPRMKEVMRVASL
                     VAKTDATVLIYGESGTGKELLACSIHRQSSRRDKPFVVVNCGAITETLLESELFGHEK
                     GAFTGAQARKLGKFELADEGTIFLDEIGEMSPAMQVKILRVLQERNFERVGGTSVIQV
                     DVRIIAATNRDLKKEVREGKFREDLYYRLNVVPIELPPLRERKEDLPLLCEFIIENHA
                     QKLHKKIKGISPQAMRLIKKYNWPGNIRELDNVIERAIILTEDEIIGVEDLHIFEAPR
                     EPHWKSLKELEKDYIEEVLEYTGGDIEKAAHVLGISPEELEKRKT"
     gene            complement(10343..10741)
                     /locus_tag="PKN31_03385"
     CDS             complement(10343..10741)
                     /locus_tag="PKN31_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOA99006.1"
                     /translation="MISYWKGLGQFIKFNVVGIMNTAVDFGVFMILNRYLGLIYAVSQ
                     VISYSCGMVNSYFLNKFWTFQKREGFTAIEVTKFILVNLCSLGVSLLVLYILQSKWSW
                     EVLPSKVLATGFSVGVNFLGNKFWVFEATV"
     gene            complement(10734..11696)
                     /locus_tag="PKN31_03390"
     CDS             complement(10734..11696)
                     /locus_tag="PKN31_03390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA99007.1"
                     /translation="MEDRVDFSVVIPVYNEEEVLPETYRRMKEVMSSAPGSYELIFVN
                     DGSRDKSQEILESFARGDEAVKLINFSRNFGHQVAITAGLDYAKGQAVIVIDADLQDP
                     PEVILTMIEKWREGYEVVYGKRVKREGESFFKKFTASFFYRLLKRFTTIDIPVDTGDF
                     RLMDKKVVDALRLFREKNRFMRGLVSWVGFRQTSVEYVREKRWAGETKYSLRKMLKLA
                     WDGITSFSDKPLKIATYAGIPLSLFSLVLFLVVLFQYLGGRGTFPATSLIGILNLLVG
                     GIILIALGIMGEYIGRIYEEVKGRPLYIVESTMGFEKEGEGDFD"
     gene            11963..>12794
                     /locus_tag="PKN31_03395"
     CDS             11963..>12794
                     /locus_tag="PKN31_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOA99008.1"
                     /translation="MKKRNKVILPLILAIGGILAVFFFSPRFSSSGQQTTSTANMPTA
                     KATRGTIQQTVSELGTLEPQNRIELKADVSGEVVKIFAQAGEKVQKDQPLLQLDTTKL
                     ETSLRKAKAQLKSAQADLKKLLAQPKEVELRQAEAQLKEAQIAYQSAKKTLAQNQELF
                     KSGGISQETLEQSEDQVTLKEALYLSAQAQFEDVKDGAAPEDIEKLQAQIEQIEADIE
                     SIENDLEKCTFKSPIDGTLLVVDPEEGDSVLSEERVVAIGDLSTMKVKILINEIDVPE
                     V"
BASE COUNT         3789 a         2989 c         2346 g         3570 t
ORIGIN      
        1 taaatttcga gaatgacgga taagagagct cttcggctct ttttcatttt ctctctttga
       61 gggaagaagc tatcttcttt tgctctcctt ccggagaaag cctcacatat tctttctctg
      121 gagagagtta gagtgagaca aatcagacta taaggggatt gctcctctat tattataaaa
      181 gattaacagt tgagcaatcc ccttataaaa aacgcctgta agaagttatt cttctcttct
      241 accttttaga tactggtcaa agaaaaccgc gagaatgata agcgcaccta ttataatttg
      301 cataataaaa gggtctactc ccagcaaatt accgccattc ctcatggttt gaattatcaa
      361 agcaccaaga gctgtcccaa aaactgttcc cacaccaccg ctgaggctcg ctcccccaat
      421 aaccactgag gcaattgcgt caagttcata tccctgcccc ccggtgggaa ctccagctgc
      481 cattctggta gccaacatca aaccagctat gccactaaag agagcagcaa cggtgtaaaa
      541 actatacata ttaagcctta tatttattcc cgatagcctg gccgcttctt tgttacttcc
      601 tatggcatag aggtttcttc caaacatggt gtatttcaat acaaaagcca taaaaagagc
      661 aagtattatc cagatccagg ccagagctgg aatgttgaga aaggtggcca ccgagaactg
      721 cctgaacccc atcggaatac cggtaatcat cctcccacta gatacataca taaccagccc
      781 cctaaccaga gtcatcattc ctaaagtagc aataaaagga gggactttgc catcgtgcac
      841 cgctactcca ttagcaagcc ctatcagcgc tgcaatcccc agagatatga gaatggcaag
      901 gcctgaagac atctggattt cgcccctatt agaggtcaga atggcatagg tcatccccga
      961 taagccgaca atcgaaccca cagaaaggtc aatccctccg gttatgatta tcaaagtcat
     1021 acctatagcc accactccat taatagaaga ctggcgaagt aaattagata tattctccca
     1081 ggaataaaag ttgccctttg tcgctatgga caaaaatatt gccatcaaaa ttattattac
     1141 caaaaggttc cgctcagcaa aggctctgcg cccacctttt tctgccatat cagactgcct
     1201 cctggacttt ttctctgtta agtatgtttt tcatcgccag ctttaagagg tattcttcag
     1261 aaaaatcctc tttttccact tcaccggcaa tttcaccatt agaaatcacg atgattctat
     1321 ccgataaacc aatcaattcc ggtagatacg atgaaattaa tataatcccc tttccctgat
     1381 caagtatttc accaaaaagt ttataaatct ccgctttcgt gcccacatca ataccagtag
     1441 tgggctcatc aaatatgaga atgtcggggt ttttacacag ccatcgagcg attactacct
     1501 tttgttggtt tcctccactc aactccgaaa gcttctggcg cacgctccct acttttatgg
     1561 aaagtttatc aacgtaatcc tgggcttgct gataagcttt gctataatct attatccccg
     1621 ccctcattac tttgtctaaa ttaatgacat ttatattgaa ttccacatcc tgctgggaaa
     1681 atattccctg attttttcta tcctctggaa gataaccaag tccgctattc agtgaatctt
     1741 tcggcctctt caccaaaatt tcctgcccgt taactacaat ttttccttca tcaaaacggt
     1801 cagcaccata gatggctctg gcaatctcgg ttcttccaga ccccacgaga ccaaatacac
     1861 ctaaaatttc ccctgctcta acattaatct caatattccg aaatctcttt cccgaaagat
     1921 tctcaacccg taagagctct ggaccgattt ctacttcctt tttgtagtac atttcttcga
     1981 tggaccttcc caccattaaa ggaatcaatt tatccacgct ttccagctcc gaaacggtgt
     2041 aagtgcctac taatttccca tctttgagta cggtcactgt atcacaaacg tcaaaaactt
     2101 cttctaagtt gtgcgatata taaattacgg ctacgcctct ttttttgagg tctctaataa
     2161 ttcgaaataa aatctgagtt tcattttgag ttaaaactgc agtgggctca tcaaaaataa
     2221 ctaattttgg ttcctgccag aggcacttgg caatggtaac catcctgccc tctgccacgc
     2281 tcaactccct tatattcctt ttcacatcaa tttccaattc catatcgcgg aggaactctt
     2341 ctgactcttc atacatcttt ccccagtcaa tgatcaatcc ctttttaggt tgattaccca
     2401 taaagaaatt ctctgctacg ctcaaatctg gagcaatcat tacatcttga tagacagcat
     2461 aaatccccag ctttttggaa acgatgggag agtttcttac aatttcgtta tcctcaaata
     2521 tgattttccc cgaatcctta tcgtaagcac cagtaacaat ttttataaga gtcgatttcc
     2581 ctgctccatt ttcacctacc aatcctctta cctctcccgc cgaaactgac atgcttactt
     2641 gatctagggc tttaactgca ggaaaagatt tgcagatgtt ttccagtttt aaaacctctt
     2701 ttttgctgga cataaaacct ctcctttaga ttaccaattt taaaaaaggg ggcggcatct
     2761 gccacccccc taaaaaataa tcagtatcag tattttttcc tggagaaagg atcgagaatc
     2821 cacattgatt catcgaggtt ttcctgggta attacgtaaa ctcccgtatc aaagtattcc
     2881 ggaagaggtt cgccattgat agacataaag gcgaacataa cgcccttata acccattcca
     2941 tgaggatcct ggacaatcag agcttttaga gctccgcttc ggagagcatc aatttcttga
     3001 gggtcagaat catacgctac agctactatc tggtcctgta aaccgcgctc ttcgataacc
     3061 cgggcaacac catcaccagt atgattgtta ttggcaaaga aacctaataa atcagggtta
     3121 gcagtcaaaa tatcttcggc aatattagct gcaaccgcaa tatcattgtc tgcgtatctt
     3181 acgggaagaa cttcaaggcc tggtgcaatt tcttctagtc gctcaagaaa cccttcggtc
     3241 cggtcaatga gaacctgaac cccggccatt gcgcttatta cagcaatttt gccctctaaa
     3301 ggcttaccgg ctgcctgaat agcctcaacc agtttatcgg cagctaaagc tccaccgact
     3361 ttattgttag tggccagaaa agagtggtag cccgtgtcgt ggacaaaatt atcgatgaga
     3421 atgattttta tcccttgctc ataagcatta ttgagaacca atgaagtggc atctgaactg
     3481 gtagatgcaa taacaatggc atcgggcttg gtgttgacca cattctccac aatagccact
     3541 tgctcatcaa tatcagcttc agatggtggt ccataaacgg ttatatttat aagacctttc
     3601 aggtcatatt ctgcattttt cgctcccact atagtatatt gccagaagtc agaatcagta
     3661 gcctttacga taaaggcaat gtcaaaaggc tcaacatcag ctgcgtaagc aaaggttact
     3721 cccagaagga caaacgccag aataagcgga attaatctct ttcgcataat ttatacctcc
     3781 ttttttcaaa gtacttcata gttaaacact aaagattcca cccttatcac acaacctcct
     3841 ttttatcact ctcctattga gtatctatcc ttttaaattt tctcaccccc ctattaggtt
     3901 aacatatttt atggtaatta ccatcaatta cagtatatat tttttgctta gaagcgtcaa
     3961 gggttaacga gactccgttt tttatatttt cattcggtaa aaatgtttgc gctttgctca
     4021 aaatcaattt tcaattataa ttttcaaggt acttaagatt aaagaataac gagagaggtg
     4081 aagaaatgcg cagttatcgc aaagagttgt ggttccaaac cgaaaaaaga agagaattta
     4141 tcaatatcac tcccttagtt gaagaatgcc ttcgggaaag cgggattcaa gaagggctgc
     4201 tactctgcaa tgccatgcac ataacagcaa gtgttttcat aaacgacgat gaaagtgggc
     4261 tccaccgtga ttttgagcgg tggctggaaa aactggctcc tgagaagcct tatgaccaat
     4321 atgaacacaa cgtgggagaa gataatgccg atgctcacct caaaaggact attatgggga
     4381 gagaggtagt tgtagctatt actgatggtc gcttggattt gggtccctgg gagcagatat
     4441 tttacggtga gtttgatgga aaaagaagaa aacgagttct ggtaaaaatt attggagaat
     4501 gaattctgct tttctatctc actccagaat caacacttcc ctgagctcca gagcgctttc
     4561 aaacatcact gtatcattga aaaaaagagt ctctctttga ggtactctta agctctttgg
     4621 ctaactcttt tattttccgg ggagatacca gtaatggtag ctcctctttc catggagagg
     4681 aaaatataat ataacatatc tttgagccac agacgtgtta tgaaaaggac caatttacaa
     4741 agcatttaag agtcatatag gaagctcttc aaagccgcac cccggagtca gtcagcagca
     4801 tcagtctgac ctttcctggt ttatgcttcc ttcattttta agaaaaattg atgagaacta
     4861 aaaactatcc tgatccacca ggagatctga gagctgagaa ataatctggt aaatatcctg
     4921 ttcctccaaa ccaagcaagt ttacagcctg gggagaaaga gggtataata aaccgtctgc
     4981 tccgatacca atgcccatcg tcacacatat aaaatcggcc acatgagcta tagaggtaag
     5041 taccggatcg gttttggcca gctccggact atggtgaaag gcgatagctt ctaccaactc
     5101 gggggggaga ttccagtttt ctgctacttt agaacccacc agggcatgat tgaaacccag
     5161 gatttcctcc tctgcctgag aaaaaggaac tttgtctttc tccaccttgt ccattacctc
     5221 ttcataggtt tctcgtatat aactattaag aactacctta cctatgtcgt ggagcaaagc
     5281 tgcagtgtaa gctacctccg ccttggaaaa ctttattctc cgggcaatta gtcgagcagc
     5341 catagctgct gattgggagt gcctccacag ttctccaggt aaaagtgcat aaccttccac
     5401 ttcctgactg agaagcttgc ttaccgaagc agctataaca atagagcgaa tagtctgaaa
     5461 gccaagcata atagtagcat cggtaacgga agaaatgcga cgggggaaac cgtaaaaagc
     5521 tgaattggcc aacctcagaa ccttagccgt catgctctgg tcttgattta ttacttcatt
     5581 aatatcccgg gcagtagaat tagggtcttc ggtaagttcc ataactcttt ttaccaccga
     5641 aggcaaagca ggaaggtcat caactttttt gagtaaatct tgaagagaaa ttttacccat
     5701 ttttcttttt ttcctttcga ccttaaaaat agctaaaact tttgaaaata tttctccttt
     5761 ataagaaagt cagagaaatt ttcttgccaa gtgttagctt tttatcaatc atcaggcaaa
     5821 tcatttgacc acatccggat tgacaagatg aggaggggtt tctccctgta acgccaaaac
     5881 caggttgcgc gccgcgagaa ctgccatttt agtgcgggta gcatgagaag ctgaaccgag
     5941 atgaggcgtc agtactacct catccagccc cagaagctct ggttctattt gtggttcttg
     6001 ttcaaaaaca tccagagccg ccccccgaat ccacccttct ctcaaggctc ggaccagagc
     6061 tttctcatct attacaggcc cccgagaagt atttataaga taagattcct ttttcatcat
     6121 cttcagttcc ttttctccta taagatggta agtttcccga gtaaggggga catgaagact
     6181 tacaaagtca gattgagcta aaagctcagg gaaaggatga taagtcactc ccagctcttt
     6241 ctctatatct tttacctggt ggtaatcaaa gtaaattatt cgcatgtcga atcctcgagc
     6301 tctccgagcc atagcttggc caatccggcc cagccctatg attcccaaag tagctccata
     6361 aacatcatcc cccagaagaa gcagcggttc ccatcctcta aattttcctt ctcgaacaaa
     6421 cctatcacct tctaccactc gacgagctac agccataagc agagcccagg caaggtcagc
     6481 ggtggcctct gttagcactc caggagtgtt ggtaaccatt attttccttt tagtggcttc
     6541 ttctatatca atattatcat atcccacagc gtagttagaa atcacccgta aattttgccc
     6601 tgccgcctcc ataacttccc aatcaatatc atcattcaga agacacagaa tcccatcgac
     6661 tccttttact ttttgaagga gaatttcccg gggcatcacc ccatcgtagt cgcttacctc
     6721 tacctggcag ctctcctcca ggatgttcag cccttcctgg gggattcgac gagtcaccag
     6781 aactcgaggt tttttatcca atttcacacc ttcctctccg gaaaatgacc aaagcttgat
     6841 atttatcatt attatactac ttttcgtgat ttttcacctt gaaaaagaaa gtcacattcc
     6901 ctctgcaaaa gcgaatagtt ccccttcttg agcttctcta taaagagcaa tatactgcag
     6961 aaactatccg ctttctcact tttgtgatga atgatgaaga aaatccgggg tatccttcat
     7021 taatcctgag gggaaatcaa aaatagaaaa actggttctc ttacagagac gggggaaagt
     7081 ttataaccat ttctaaaacc acagtaaatc aacccccgga gaaaaaaagt acacagccta
     7141 cccttcagat aataaaatag tcgctcttta gatggctgga aggtttggcc tgaacactcc
     7201 agcgcattct tactttgttt cctcactata gagataaagc tacacagaca acaaaagcaa
     7261 ttagctccct ccttaagaac gaaagcaacc tagcatcttc tcttcgcctt aaccatacgt
     7321 gaatctcttt ctgtcaggtc attatcgaaa gaaatctgga agtcctctat aaactgagaa
     7381 tatcttttta gtatttgatg agcctatcca gcgaattctt agctttaccc cgtttccttt
     7441 taccccgtcc gggcctatct ctttcacctc tctgggatag tccttgagag cttnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttaccc cgtccgggcc tatctctttc
     7621 acctctctgg gatagtcctt gagagcttga gtgagaatag tctgatagcg gtgatgatgg
     7681 ggacttacca gttccggctc tcggtcctat ttaccggcat atttagtttg ttctttcatt
     7741 ttagaccatc aaaatcccct tatccataat caactctccg tcaaacttca ctgtggggtt
     7801 gcgaattatg ccatcgttat gcgttttaga ttctaccttg ccacctatac tcacgttcat
     7861 acccaaagca atgtgcacgg tgcgaagagc cttttcatct tccagaatat tgcctgtgat
     7921 acgtgccttt tcattggtgc caatgcccag ctccccaacc cagtaagcat aggggtcaga
     7981 taattcctca agaaaccggg aaagctcttc tgcttctctt cctccttcta tttttatcac
     8041 tttccctctc tgaatggtaa gcttaatcgg ctcagccaat agcccaagag gcgacataga
     8101 accatcaacc accactattc cctctaccga ttcctctacc ggagcaacaa aagcttcccc
     8161 cgcaggtaaa ttgccccatc tcccccgctc ccggtacaag ccatcatcag gaggaattgc
     8221 cggttttctg cccttcaagc tcaagcgcac atcagttccc ttttccgtat atatctccac
     8281 aaggtcggtt gcttccagaa gatgggttag cttccagctc agctccgaaa ctttctcgta
     8341 atcggcagtc atagctccca cagtaagcat atcttcagta atccccggca tgctggctat
     8401 gcgcacacct ttttttgtag cttccatgcg agcataggta tggctcaagc tcgtagtggt
     8461 aacaagaagt gccacatcta ccgatgccat agctacccca atttccgagg gtggctcctc
     8521 aacatggtga gagcgaggaa ccatcaggga tagagtagca tctactccta aagcatggag
     8581 agcaaaaaga aagctcttag caatatcgat tttacctgtg tcggtaatta ccaccacttt
     8641 ttcttccttt ttcaccccca tacaatcccg aaccgcaatc attgctcctt tcattaaagg
     8701 agtagttttc aacctcatct acctccttaa tttcccatgt tccttttact tttttattat
     8761 cacgagatat cacgagaaat atgtttattt tttctgcccg caaagcatga ggttcacggg
     8821 caaacacgat aatcgtttat ttttcctctt taatcgcttg acaagtcaga tttttatccc
     8881 cggcaacatc tttcccgcga ttcacctcgc tatttcaacc aagtatagca taggaatttt
     8941 acattttttt aatttttccc gaaaaaaatc aaccatacta tctttactat atttactata
     9001 taatattaat gctctatgga ggaggtgagg ttttgccagt ttttagaata ttgattgtcg
     9061 atgatgaaga atccttaaga gagatgttaa aagagttttt agaaggagag ggaatgctgg
     9121 tagaacttgc tcaaaacggg agagaagctt tagaaaaatt gcattccctg agttttgacc
     9181 tggttttgtt agacttacgc atgccgggag ttagcggtgt agaggttctg caagaaataa
     9241 aaaaagagga acctgagcta ccggtagtgg tcatcactgc ttatggtagc gtagataacg
     9301 cagtagaaac tttgaaaatg ggagcttttg actttattac taaacctttt aagctggaag
     9361 aattattgaa taccatctcc cgggctttag aagtagaacg actaaaaaga gagaaacaat
     9421 atcttctgga ggaaatgcag gacaaattct gctttgaagg agtagtgggt caatctcctc
     9481 gaatgaaaga agtaatgcga gtagcttcgc ttgttgcgaa aaccgatgcc accgtcctga
     9541 tttacggaga aagtggaaca ggaaaagagc tattagcctg ctccattcac cgccagagca
     9601 gcagacgaga taaacctttt gtagtggtaa attgcggagc tattactgaa actcttttag
     9661 aaagtgaact gttcggtcat gaaaaaggag cttttaccgg agcccaggcc agaaaactgg
     9721 gtaaatttga gctggccgat gagggaacca tttttttaga tgaaattgga gagatgagtc
     9781 cggcaatgca ggtaaaaatc cttcgagtat tgcaggagag aaactttgag cgggtaggag
     9841 gtaccagtgt aattcaggtg gatgtgcgca tcatagctgc tactaatcgg gacctgaaga
     9901 aagaagtgcg agaaggtaaa tttcgtgaag acctctatta ccgcctgaat gtagtgccta
     9961 ttgagcttcc tcccctgaga gaacgaaagg aagaccttcc ccttctttgc gaatttataa
    10021 tagaaaacca tgctcaaaaa ttacataaga aaatcaaggg gatatctcct caggccatgc
    10081 gccttatcaa aaaatacaac tggccaggaa atatccggga actggataat gttatagaac
    10141 gagccataat tttaactgag gatgaaatta tcggagtgga ggacttacat attttcgaag
    10201 ctccacgaga gccccactgg aaaagcttga aagagttgga aaaagattac atcgaagagg
    10261 ttttagaata cactggagga gacatagaga aagccgccca tgtcttagga atttcccccg
    10321 aagaactgga gaaaagaaaa acttagacag tggcttcaaa tacccagaac ttattaccca
    10381 gaaaattgac tcccaccgag aaaccagtag ctaaaacctt actgggtaga acttcccagc
    10441 tccatttact ttgcaaaata taaagcacca gaagagaaac ccccaacgaa cataaattga
    10501 ctaaaataaa tttagttacc tcaatagcag taaaaccttc tcttttttga aaagtccaga
    10561 atttattcaa aaaatagcta ttaaccattc cacaggaata agaaataacc tgcgacacag
    10621 cataaatcaa ccccaggtaa cgattaagaa tcataaaaac tccaaaatcc acggcggtat
    10681 tcattattcc caccacatta aacttgataa actgcccgag acccttccag tagctaatca
    10741 aaatctcctt ctccttcttt ttcaaaaccc atagtacttt ctacaatgta aaggggacgt
    10801 cccttaactt cttcataaat tctacctata tattctccca taatccctaa agcaataagg
    10861 atgattccac caaccagaag gtttaaaatg cctatcaaag aagtggcagg aaaagtgcct
    10921 ctacccccga gatattgaaa aagaactacc agaaaaagca ccaggctgaa aagagaaaga
    10981 ggaatacctg cataagtggc tattttaagt ggtttatcag aaaaagaagt gattccatcc
    11041 caggctaatt taagcatctt acgcagagaa tatttggttt ctcccgccca tctcttttct
    11101 cttacatatt ccacactggt ttgacgaaag cccacccagc tcaccagccc tctcataaaa
    11161 cgattctttt cccggaagag cctcaaagca tcaactactt tcttatccat caagcggaaa
    11221 tcaccagtat ccaccggtat gtctatagtg gtaaatcttt tcaagaggcg ataaaagaaa
    11281 gaagcagtaa actttttaaa aaaactttct ccttcccgtt ttactctttt tccgtaaact
    11341 acttcatatc cttctctcca tttttctatc atggttaaaa taacctctgg tgggtcttgc
    11401 aaatcggcat caattacgat tactgcctgt ccttttgcat agtccaaccc tgcggtgatg
    11461 gctacttgat gaccaaaatt acgggagaaa ttgattagct taaccgcttc atctcctcgg
    11521 gcaaagcttt ctaaaatctc ttggctttta tctcggcttc catcattaac gaagatgagc
    11581 tcatagctac ccggggcaga gctcatcacc tccttcatcc ttctgtaagt ctcgggaaga
    11641 acctcctcct cattataaac aggaatgacc actgaaaaat caaccctatc ttccatatcc
    11701 atctccttga tttttatttt agctctattc tactctttaa ggtgttattt cgcattactt
    11761 tctcaatgtc tgtgattttc ataaattggt cacaaattgc tcaccaaaaa gccccaaaag
    11821 cgtcttataa tttccccgga aaaaggaact taaaaacaaa aggttggtat tttaggtata
    11881 ggttagttta acaaagtttg actttctcta tttgaccacc aagctataat agacaataat
    11941 gtagaaaagg agcaaaatat aagtgaaaaa acgtaacaaa gttattcttc ctctcatttt
    12001 agcaatagga ggaattctgg ctgtcttttt cttttctccc cgtttttcct cttcaggaca
    12061 acaaactacc agtacagcca atatgcctac tgctaaagca accagaggca ccattcagca
    12121 aaccgtatcg gagctgggga ctcttgaacc ccagaaccga atagaattaa aagcagatgt
    12181 gagcggagaa gtggtaaaga tttttgccca ggcgggagaa aaagtgcaaa aagaccagcc
    12241 gctacttcaa ttagatacta ccaaactgga aacctctctt cgaaaagcaa aggcgcaatt
    12301 gaagagtgct caggctgacc ttaaaaaact cttagctcaa cctaaagaag tcgagctaag
    12361 acaggccgaa gcccagctaa aagaagctca aatcgcctat cagtcggcga aaaaaaccct
    12421 ggctcaaaat caagaactat ttaaaagcgg tggtatttct caagaaacct tagagcaaag
    12481 cgaagaccag gtaacattaa aagaagcact ttatctctcc gctcaagctc aatttgaaga
    12541 tgtcaaagat ggagctgccc cggaagatat agaaaagttg caagcccaaa ttgagcaaat
    12601 agaggctgat atagaatcca ttgaaaatga tttagaaaaa tgtaccttta agtctcccat
    12661 cgatggcact cttcttgtgg tagatcctga agaaggagat tcagtcctct ctgaagagag
    12721 agtagtcgcc ataggagacc tctccaccat gaaagtaaaa atattaataa atgaaatcga
    12781 tgtcccagag gtgg
//
LOCUS       DAOPSS010000034        12770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4137_length_12770_cov_7.320252, whole genome shotgun sequence.
ACCESSION   DAOPSS010000034 DAOPSS010000000
VERSION     DAOPSS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4137_length_12770_cov_7.320252"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1180
                     /locus_tag="PKN31_03400"
     CDS             158..1180
                     /locus_tag="PKN31_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOA99009.1"
                     /translation="MSVTIKDVARLAGVSPATVSIVLNGKGKVSEETRKKVEEAAQKL
                     GYRPNIVARSLVRGKTNTILLCAFIKEQEKLSAFYGELINRLLAAISREGYYLQIVVK
                     GEFFNGHPLDKREALLNIARNSLFEGLVVLSHWPIHYSEVSDLVKENFPFVIVNQRVE
                     GEGVSYVDIDHYGGAKEAVKYLIEKGHRQIAHLRGPREHRHAEERYRAYIDTLLDFGI
                     PIKKEYVVEGNFRRMSGREAMEKLLQISPLPTAVFAANDKMAIGALQVAKERGIKVHD
                     DIAIVGFDGIEAVKYTDPPLPTVEQPLGELGKVAAELLVESIKGGEVKKIVLPCRLTE
                     WEVNHL"
     gene            1364..3208
                     /locus_tag="PKN31_03405"
     CDS             1364..3208
                     /locus_tag="PKN31_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99010.1"
                     /translation="MGFAQAGFAQLAEDIPREKTFIADVLTGRVGTPANFNVFTTAWR
                     NPDRGVQQLMLEPLWLVDYVTGEVINALAAEGPIYNEDFTQMTVKLREGCQWSDGVPI
                     TADDIVYGIELCMQDSGMSYNAPFNLYVDKVYKTDDYTVVFEFKEPNARFHANFIDRW
                     GGWRPFPKHIFEEAEDPLSFDFNPPISSGPYVLKDFDQAGYWTLWERREDWDKTPTGI
                     LYGMPQPEYVLFYYYGDAANKVIAQANHNLDMTDLTMEAFRAILERNEYSRGYRKEYP
                     WVVNVDPCMTGLTLNNDVSPFDLKDVRWALTLAIDIVDYIGIAFDGAAPMGVLHVPPT
                     PVYQEWYYKPLEEWLKNLTLDIEIDGEPFKPYDPEAPFRLVEYVKERGYDVPKEPEAI
                     REMFGPGWWKYAPDVAEQLLLRNGFTKDANGKWLLPDGTPWKIAITAHPSPSHPQHRN
                     SLAAAQQWRNFGIDVTVNPSEAYAALAQTGDYVVNDNWPAIEPWGGHPDLYRVLSPFR
                     TSYYRPIGEQAVGHSSRWYNEKLDKVIEEMEKVDWDSDKNLQLGMEGLKILVEEMPTI
                     PTFGYPGVVAWDEYYWTNYPGAENPYTQPYHHWPNFKYMLPFLRPVER"
     gene            3273..4280
                     /locus_tag="PKN31_03410"
     CDS             3273..4280
                     /locus_tag="PKN31_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99011.1"
                     /translation="MLFFKRYLLPRIVQFLVVVFVGITIVFIVPRLTPVDPVQQVINR
                     ITAQGAFLDPAAVDSMRKSLTELYGLEGSMLQQYFAFWGRLFRGDFGPSLFQFPIPVT
                     QLIMTSLPWTAGLLISTTLISWIVGNLLGGLAGYFKDRRWAKVLDNISMVIRPIPYYI
                     FALLLLILFAYLFPIFPSAGGYSVGRRIGFNWAFISDVLRHSFLPATSLVVLGIAANF
                     MTMKLIVSNIVAEDYVMYAKAGGLKESKIAFKYVLKNGLLPQITGLALSLGQIFGGAL
                     ITEIVFSYPGIGTLVYGAINSGDYNLIMGVTSLSVIGIAGATLIIDLLYPLFDPRIRH
                     R"
     gene            4291..5151
                     /locus_tag="PKN31_03415"
     CDS             4291..5151
                     /locus_tag="PKN31_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99012.1"
                     /translation="MESFKELMKDGRFKFAFIVICVLALMSILSFFSPYNPLKWNVVP
                     RDLPPRWPHILGTTSQGQDLFWQSTFAIRNSLIIALIASSISRVIAVIVGLIAGYKGG
                     RVDTILMSINDSFVVMPLLPILILIASVMRENLTMVTLGLILSLFGWAWDARVIRSQI
                     LSLREREFTYTAILSGTRTFSLVIKEYFPFIIPIMFSTVINNMIWAIGMEVTLAILGL
                     TNIEVPTIGTMIHWAVNYQSMLLGYWWWILTPVVISILLFVALYLLSSSLSEYLDPRT
                     RIQRIGKAGQ"
     gene            5154..6149
                     /locus_tag="PKN31_03420"
     CDS             5154..6149
                     /locus_tag="PKN31_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99013.1"
                     /translation="MDILKTENLKSYYILEMGETKKEVKAVNDVTINIHEDEIYGIAG
                     ESGCGKSTFLKTICKLVEPPLRMIGGTIYYQIDGKYVEVTEIKEEDFRKLRWKYISYI
                     PQGSMNALNPIIRIRESFKDFFTAHQKIENEERDFEEPLKKHLSALGLPVRVLKSYPH
                     QLSGGMRQRTTIALATILKPRIIVADEPTTALDVVAQRAVIQLLKDIQKLQKNTIILV
                     THDMAVHANITDRIGIMYAGMFVEEARTDDIFENPLHPYTKFLIASLPKMGDKTYKPS
                     VPGVPPSLSNLPPGCPFHPRCPYAMDICKKEIPQLVELNTEHKSACFLNSKERVE"
     gene            6146..7147
                     /locus_tag="PKN31_03425"
     CDS             6146..7147
                     /locus_tag="PKN31_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99014.1"
                     /translation="MNRKNDNPEKEIEKEGLIKAKKLQKVFVVGGGFARSRLLAVDDV
                     SFQVNKNEIFTLAGESGCGKTTVSKILLGFLEPTQGEVYYRDKNILELQKWEEKKQFM
                     KEVQSIFQNPFETFNPLRKVDSYLHDTAKNFGMNGKKRDKKAVEEALQSVGLGMEEVQ
                     GRYPSEFSGGQLQRISIARALITKPSLLVADEPVSMVDASLRMSIVNLFKELRDEHSV
                     SVLYITHDLATAYYVSDRIGIMFRGNIVEMGDVEEVLMNPKHPYTQMLKESIPEADPK
                     KKWTERVSITELETEEYMRKGCKFAGRCPFVEEKCKDFMPEEFFVDGRMVRCWLYEK"
     gene            7293..9554
                     /locus_tag="PKN31_03430"
     CDS             7293..9554
                     /locus_tag="PKN31_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOA99015.1"
                     /translation="METTTKLVNDLLSRMTLEEKVAQLGSVQARHLLENGQFSREKAR
                     ELLKNGIGQITRAAGGTGMEPEKVAELVNEIQKFLREETRLGIPAMIHEECLSGFTAW
                     GTTAFPQAIGMASTFEPEVIQKMTSVIGKQLRAVGAHQGLSPVLDIPRDPRWGRTEET
                     FGEDPYLVSQMASAYIKGLQGEDFESGIIATAKHFPAYGISEGGRNLAPARVSERELR
                     EIFLFPFEVAVRETRVGSVMNAYHEIDGIPCAASQLFLTKILREEWGFEGLVVSDYGA
                     IRMLENFHYVADSAQEAAIKALEAGIDIELPNIDCYGEPLLEAVRKGLISEEVINESV
                     RRMLRAKLLLGLREREKIEISLQEAKKALDSPEDRELSREIARKSIVLLKNDGVLPLK
                     KDSGTIAVIGPNANSTRNLHGDYSYTAHVASEEDAVETISVLEGIKNKVSANTKVLYA
                     QGCDLLDTSCDGFQEALELAEQSEVIIAVMGENSGLFKNSISGEGSDRVDLDLPGVQR
                     ELLQALKETGKPIVLVLVNGRPLSITWEKDNVSAILETWYPGEEGGNAIADVLFGDYN
                     PGGKLPISFPRSVGQIPVYYNRKPSSFNEYINTDTQALFPFGHGLSYTEFSYSDLKIT
                     PSQVNSLEEVKISFKVKNTGSREGDEVVQLYIHDRVASVERPVKELKGFKRLTLQPGE
                     EKEVIFTLFPEQLAFYDEFMRLIVEPGVFEVMIGSSSEDIRLSGEFEVLKEKVITKYR
                     KFSSEVIVSRKTY"
     assembly_gap    9661..9760
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10175..10561
                     /locus_tag="PKN31_03435"
     CDS             10175..10561
                     /locus_tag="PKN31_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOA99016.1"
                     /translation="MKFQGLHHVAIKTNDLDKTLDFYCGILGLEVEERFYDAGEQSDI
                     VFLSLGNTLLEVIAPHRPEEKELNFSTCFHLALATSNVEETYEKLKEKGINFTLPLTE
                     SGGFKYAYFSGPMGETLEIVERIKAK"
     gene            complement(10554..10862)
                     /locus_tag="PKN31_03440"
     CDS             complement(10554..10862)
                     /locus_tag="PKN31_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99017.1"
                     /translation="MKETCEFEKWLEQIEEKIKELPVEEKLEIIAEEIYSRYDARVWF
                     SEISGKRWSYIAGWGGYDPTPVHQVLLSPRYGMTIEEERIPEAEREALLSFLKRGLFT
                     "
     gene            complement(10840..12297)
                     /gene="hydG"
                     /locus_tag="PKN31_03445"
     CDS             complement(10840..12297)
                     /gene="hydG"
                     /locus_tag="PKN31_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOA99018.1"
                     /translation="MSVKEWMGNIIKEEEDEKFLEGGKDFINDEEIEELLQKNRNPEP
                     QKVRDIIAKSLSLERLEPEETATLLNVEDEDLWEEMYRAGGEIKRSVYGDRIVTFAPL
                     YCSNFCVNSCLYCGFRRDNNLQKRHRLTLEEVRRETETLVSLGHKRLVVVYGEHPLSD
                     ADYIASTIQTIYDTKVGNGEIRRVNVNAAPMDISRYKILKEVGIGTFQVFQETYHHET
                     YRQLHPSGIKSNYQWRLYALHRAQEAGIDDVAIGALFGLYDWKFEVMGLLYHTIDLEK
                     HFAGVGPHTISFPRLEPAVNTPLVEETKYRVSDRDFKRLVTVIRLSVPYTGMILTARE
                     PAHIRRDVIPVGCTQIDASTRIGIGAYSDNSNEQELERQQFQIGDPRSLDEVIRELAQ
                     MGYITSFCTADYRCGRTGKYFMDIAKKGKIHYFCIPNAILTFQEYLEDYASPQTKEIG
                     EKVIQEHLSSLKDGIKPKVEEYLQRIKKGERDLRV"
BASE COUNT         3862 a         2540 c         2762 g         3506 t
ORIGIN      
        1 ccatgtagct tgaagaatga gacttgctat atagcaaaag agatggtttc taatcgtttt
       61 aactcgcgac tttttttctg cttaccattg acaagtaatt tagatataat ttatactaac
      121 atcaatctat taaaacgatt tattcaagga gaaaaacgtg tctgtaacta ttaaagatgt
      181 agcaaggtta gctggtgtat ccccggccac agtatcaata gttctaaatg gaaaaggtaa
      241 agtttcagaa gaaacccgaa aaaaagtgga agaagcagca caaaaattgg gatatcgccc
      301 caatattgta gctcgaagct tagtaagagg taaaaccaat accattttgc tctgtgcttt
      361 tattaaagag caggaaaaac tttctgcttt ttatggagaa ttgattaacc gccttttggc
      421 tgccatttct cgggaaggat attaccttca aatagtggta aagggagagt tctttaacgg
      481 tcacccttta gataagcgag aggctctttt aaatattgcc cgtaatagtc tctttgaagg
      541 tctggttgtt ctttctcact ggcctattca ttattctgaa gttagtgact tagtcaagga
      601 aaacttccct tttgtcattg ttaaccagcg agtggaagga gaaggagtaa gttatgtaga
      661 tattgaccac tatggtggag caaaagaagc agtcaaatat ttaatagaaa aaggtcacag
      721 gcagattgct catctgcgag gtcccagaga acatcgccat gcggaagaga gataccgagc
      781 ttatatagac actcttttag attttggtat tcccattaaa aaagagtatg tagtagaggg
      841 gaatttccgc cggatgagtg gaagagaagc aatggaaaag ttgcttcaaa tttctcccct
      901 tcccactgca gtttttgccg ctaacgataa aatggctatc ggcgccttac aagtagccaa
      961 agagagaggc attaaagttc acgacgatat cgccatcgtc ggctttgatg gaattgaagc
     1021 agtaaaatac actgacccgc ccctccccac agtagagcaa cccttaggag agctgggaaa
     1081 agttgctgct gaacttttag tagagagcat aaaaggaggt gaggtaaaaa agatagtttt
     1141 accttgccgg ttaacagagt gggaagttaa ccatctttag ttttccaaaa ggtggatgca
     1201 tcaaagtata accagagtaa ttcctaccaa aaagataggg ggtgagaaaa actcagaaac
     1261 gacagtttaa gcacaaaaaa ataaccagat tatatttaaa atggaggtgt gattagtgaa
     1321 aagaagtttt tcaatctttt ttgtgtcttt gttagtactg gtgatgggct ttgcccaggc
     1381 aggctttgcc caattagctg aagatatccc cagagaaaaa acatttattg ctgacgtatt
     1441 aacagggaga gtaggtactc cagctaactt taacgtgttc accactgcct ggagaaatcc
     1501 ggatagaggc gtacaacaat taatgctcga acctctatgg ctagttgatt acgtaaccgg
     1561 cgaggtcata aacgccttag ctgcagaagg tcctatttac aatgaagact tcactcagat
     1621 gacggtaaag ctgagagaag gttgccagtg gagcgatggt gtccccataa ctgcagatga
     1681 catagtatat ggaatagagt tatgtatgca ggactctggg atgtcttaca acgccccatt
     1741 taatttgtat gtggataaag tatataaaac cgatgactat acagtagtgt ttgaatttaa
     1801 agaacccaat gcccgattcc atgctaactt tatagacaga tggggtggat ggagaccttt
     1861 ccccaaacac atttttgaag aagcagaaga tcctctatcc tttgatttta accctcccat
     1921 aagcagtggc ccatatgtgc tcaaagattt tgatcaggca ggttactgga ctctctggga
     1981 gcgaagagaa gattgggaca aaacccccac tggaatattg tacggtatgc cccaacctga
     2041 atatgtgctt ttctactact atggagatgc agcaaacaaa gtaatcgctc aagcaaatca
     2101 caacttagat atgaccgatt taactatgga agcattccgg gcaatattag agagaaacga
     2161 atattcaaga ggctacagaa aagaataccc ctgggtggtt aatgtggatc cttgcatgac
     2221 aggtttaact ctcaacaatg acgtatcccc attcgacctg aaagatgtca gatgggcttt
     2281 aactctggcg atagatatag tagactacat tggaatagct tttgatggag cggctcctat
     2341 gggagtccta catgtgcctc ctacacctgt atatcaggaa tggtactata aacccctaga
     2401 agagtggcta aagaacctca ccttagatat agaaattgat ggtgagcctt tcaaacctta
     2461 tgaccctgag gcacccttcc gacttgttga atatgtaaaa gagagaggct acgatgtacc
     2521 aaaagagcct gaagcaatca gagagatgtt tggaccggga tggtggaaat atgctcctga
     2581 tgtggcagaa caattactgc tcagaaatgg atttacaaaa gatgcaaacg ggaaatggct
     2641 cctgcctgat ggaactccct ggaaaatcgc tataactgct catcccagcc cctcacaccc
     2701 tcaacatcgt aactcactcg ctgccgctca gcagtggaga aactttggaa ttgatgtaac
     2761 tgtgaatccc tcagaagctt atgcagcatt agctcaaacc ggggattatg tagtaaatga
     2821 taactggccg gcgatagagc cctggggagg acatcctgat ctctatcgag tgctgagccc
     2881 tttccggact tcctactata gacccattgg cgaacaagca gttggtcatt cttcgagatg
     2941 gtacaacgag aagttggaca aagttataga agaaatggaa aaagttgatt gggacagcga
     3001 taaaaacctg cagctgggaa tggaaggttt aaaaatttta gttgaagaaa tgcccaccat
     3061 tcccacgttt ggttatccag gagtggtagc ctgggatgaa tattactgga ccaactatcc
     3121 tggtgctgaa aatccctaca ctcagccata ccatcactgg cccaacttca aatatatgct
     3181 tcccttctta agacctgtag aaagataaaa tctgatacct taccagtgcc ccggacgggg
     3241 cactggtaag ggtaagttta gaggtggggt ccgtgctatt ttttaagaga tacttattac
     3301 caagaatagt tcagttttta gtagtagttt ttgtagggat aactatagtt tttatagtac
     3361 cgaggctaac tcctgttgat cccgttcaac aagtcatcaa tcgaataacc gcgcaaggtg
     3421 ccttcctgga tcccgctgcg gttgatagta tgcgaaaatc actaactgag ttgtatggtt
     3481 tagaaggcag catgcttcag caatatttcg ctttttgggg aaggttgttt cgaggtgatt
     3541 tcggcccttc cttattccag tttcctatcc ctgtcactca actgataatg acctctctac
     3601 cctggacagc ggggttactg atttctacca ccctgatttc ctggatagtg ggcaacctcc
     3661 tgggaggatt ggcaggatat ttcaaagata gaagatgggc aaaagtactg gataacatat
     3721 ccatggtcat aagacctata ccctactata tatttgctct gttgcttctt atactgtttg
     3781 cttacctctt ccccattttc ccttccgcgg gaggctactc ggtgggaagg agaataggct
     3841 tcaactgggc ttttatttct gatgttctga gacactcttt tctccctgcc acttctttag
     3901 tagttctggg aatagcagca aactttatga ctatgaagct tatagtttca aatatagtag
     3961 cagaagacta cgttatgtat gccaaagcgg gagggcttaa agaatcaaaa atagctttca
     4021 agtatgtact aaaaaatgga ttgttacccc agataaccgg cttagcttta tctctgggac
     4081 aaatatttgg aggagctttg ataaccgaaa tagtgttttc ctatcctgga ataggaactt
     4141 tagtatatgg agccatcaac tctggggatt acaacctgat aatgggtgta acttctctct
     4201 cagttatagg gatagcagga gcaacgttga taatcgatct cctatacccc ctctttgatc
     4261 cacgaataag acatagatga ggtgactgcc ttggaatctt ttaaagaact gatgaaagat
     4321 gggagattca aatttgcttt catagtgata tgtgttcttg ctcttatgtc tatcctttct
     4381 ttcttttctc cctataatcc tcttaaatgg aatgtagtgc ctcgagacct gccgccacgc
     4441 tggcctcaca tattgggaac tacctcccag ggacaggact tattctggca atctactttt
     4501 gccataagga attcactgat catagccctg atagcttcat caatatcgag agtaatcgca
     4561 gtaatagtgg ggttaatagc cggatataag ggaggaagag tggataccat tcttatgtcc
     4621 ataaacgata gttttgtagt catgcctctt ttgcccattc tcatactgat agcttcggtg
     4681 atgagagaga acttaacgat ggtaacttta ggactgatac tgagtttatt cggatgggcc
     4741 tgggatgcta gggttataag gtctcagata ctgagcttaa gagaaagaga attcacatac
     4801 acagcgatat tgtcgggcac cagaactttc agcctggtaa ttaaagagta ttttcctttc
     4861 ataataccga taatgttttc tacagtgata aacaacatga tatgggcaat aggaatggaa
     4921 gtaacgctgg ctatattagg tttgactaac atagaagtgc ctaccatagg cactatgatt
     4981 cactgggcgg ttaattacca atctatgctc ctcgggtact ggtggtggat attgacacca
     5041 gtcgtaatat ctatattgct attcgtagct ttatatttac tttccagcag tttaagtgag
     5101 tatcttgacc cccgaaccag aattcaaaga attggaaaag caggacaata aaaatggata
     5161 tcttgaaaac agaaaattta aaatcttatt atatcctgga gatgggagaa acaaaaaaag
     5221 aagtaaaagc ggtaaatgac gtaactatta atatccatga agatgaaata tacgggatag
     5281 ccggagaaag tggctgcggg aaaagtacat ttctaaaaac catatgtaaa ttagtagagc
     5341 cacccttgag gatgatagga ggaactatat actaccagat agacgggaaa tatgtagagg
     5401 taactgaaat aaaagaggaa gacttcagaa aactacgatg gaaatatata tcttacattc
     5461 ctcagggctc tatgaacgcc ttaaacccta taataagaat cagagagtca ttcaaagatt
     5521 tcttcactgc ccatcagaaa atagaaaatg aggagagaga ttttgaggaa ccattaaaaa
     5581 aacacctatc agccctggga ttgccagtga gagtcttaaa atcttatcct catcaactct
     5641 caggaggaat gagacagaga actaccattg ccttagctac tattttgaaa cccagaataa
     5701 tagtggctga tgagcccacc actgccctcg acgtagtagc tcaaagagca gtgatacagc
     5761 tcttgaaaga tattcaaaaa ttacagaaaa acacgataat tttagtcacc cacgatatgg
     5821 cagtgcatgc caatattaca gacagaatag gaataatgta tgctggtatg tttgtagaag
     5881 aagccaggac tgatgatata tttgaaaatc ctcttcatcc ctacactaaa tttttaattg
     5941 cttctcttcc caaaatggga gacaaaacct ataagcccag cgtaccaggt gttccaccgt
     6001 cgctctctaa cttgccacca gggtgtcctt tccatcctcg atgcccttat gccatggata
     6061 tctgtaaaaa agaaatacca cagctggtgg agttgaacac cgagcataaa tcagcctgtt
     6121 tcttgaattc caaggagaga gtagaatgaa caggaaaaac gataatccag aaaaagagat
     6181 agaaaaagaa gggttaataa aagcgaaaaa attacaaaag gtgtttgttg taggaggggg
     6241 atttgcccgg agcaggcttc tggcagtgga tgatgtatcc ttccaggtga acaaaaatga
     6301 aatattcact ttagctggag aaagtggctg cgggaaaaca acagtatcaa agatcctttt
     6361 aggctttctc gaacccaccc agggagaagt atactacaga gacaaaaaca tactcgaact
     6421 ccaaaaatgg gaagaaaaga agcaatttat gaaagaagtg caatccatat ttcaaaatcc
     6481 ttttgaaact ttcaatccct tgaggaaagt agacagttat ttgcacgata ctgccaaaaa
     6541 ctttgggatg aacgggaaaa agagagacaa gaaagccgta gaagaggctc tccaatcagt
     6601 ggggttgggt atggaagaag tacagggaag atatcccagt gaattctctg gaggacagct
     6661 ccagagaata tccatagccc gggcattaat aactaaacca tctcttttag tagctgatga
     6721 gccggtctct atggtggatg cttcactgag aatgtcaata gttaacctct tcaaagagtt
     6781 aagagatgag cacagtgtca gtgttttata cataacccac gaccttgcca ccgcttatta
     6841 tgtgagcgat agaataggaa taatgtttcg aggaaacata gtagaaatgg gtgatgtaga
     6901 ggaagtgctg atgaatccca aacatcccta tacccagatg ctaaaggaat ccataccaga
     6961 agctgacccc aagaaaaaat ggacagaaag ggtgagtata accgaactgg aaactgaaga
     7021 gtatatgagg aaaggatgca agtttgccgg gagatgccca tttgttgaag agaaatgtaa
     7081 agacttcatg ccagaagaat tttttgttga tggaagaatg gtgagatgct ggctatatga
     7141 aaagtaaaac aaacagaacc accatgcttc caggtattgg taattttcaa aaaattagca
     7201 ataaattatt gtagcagcga agagccttct tcagtcggag aaaaataggc tagaatgctc
     7261 tgctctaaat cacccaatag gaggttagtt atatggaaac tacaactaaa ttagttaatg
     7321 atctactatc ccgcatgacc ttggaagaaa aagtggctca gcttggttct gttcaggctc
     7381 gtcacctgtt agaaaacggc caattttctc gggagaaagc ccgagaactt ttaaaaaatg
     7441 gcataggaca gatcacaaga gcagccggtg gaacaggcat ggagccagaa aaagtagccg
     7501 agctggtcaa tgaaattcaa aaatttctga gggaagaaac ccgcctcgga attccagcta
     7561 tgattcacga agaatgctta agcggcttta ctgcgtgggg gacaacggcc ttcccccagg
     7621 ccataggtat ggccagcacc tttgaacctg aggtcattca aaagatgacc tcggtaatag
     7681 gaaaacaatt gagagctgta ggcgctcatc agggattatc tccagttctc gatattcctc
     7741 gcgaccctcg ctggggacgg acagaagaaa cttttggcga agacccctat cttgtatctc
     7801 aaatggcttc tgcttatata aaaggtctcc agggagaaga ttttgaatca ggtatcatcg
     7861 ctaccgccaa acattttcct gcttatggaa tctctgaagg aggaaggaat ttagctcctg
     7921 cccgggtttc ggaacgagaa ttaagagaaa tattcctctt tccctttgag gtggcggtaa
     7981 gagagacccg ggttggttca gttatgaatg cctatcatga aatagatggt ataccttgtg
     8041 ctgcctctca attgttttta accaaaattc tgcgtgaaga atggggattt gagggattgg
     8101 tggtttctga ctacggagct ataagaatgt tagaaaactt ccattacgta gccgatagtg
     8161 ctcaggaagc agccataaaa gccctggaag caggaataga tattgagctt cccaatattg
     8221 actgttatgg agaaccactg ctcgaagcgg ttcgaaaagg attgatttca gaagaagtga
     8281 taaatgaatc agtaagaaga atgttaagag caaaacttct tttaggactc agagaaagag
     8341 aaaaaatcga gataagtctt caggaagcaa agaaagccct cgactctccc gaagaccggg
     8401 agctatcccg agagatagct cgaaagtcta tagttctcct gaaaaacgat ggagtgttac
     8461 ccctgaaaaa ggattcagga actatagcgg ttatagggcc caacgctaat agtacaagaa
     8521 acttacacgg agattatagt tatacagcac atgtagcctc ggaagaagat gcggtggaga
     8581 cgataagcgt gctggaagga ataaaaaata aagtctctgc gaacactaaa gttctttatg
     8641 ctcagggatg tgatcttctg gacacttctt gcgatggttt ccaggaagca ctggagctgg
     8701 cagagcaatc tgaggttatt attgcagtta tgggggaaaa tagcggtctt tttaagaatt
     8761 ctatttctgg agaaggaagt gatcgggtgg atctagacct ccccggggta caaagagaac
     8821 tccttcaggc tctcaaagaa accgggaaac ccatagtttt agttttagtt aatggccgac
     8881 cgctttccat aacgtgggag aaagacaacg tctctgccat tttagaaacc tggtatcccg
     8941 gagaagaagg aggaaacgcc atagctgatg tgctattcgg tgattataac cccggaggga
     9001 aactccccat ctccttcccg cgaagcgtag gacagattcc agtttattat aaccggaaac
     9061 cctcttcttt caacgaatac ataaacactg acacccaggc tcttttcccc tttgggcacg
     9121 gtctaagcta taccgagttt tcttactcag acctaaaaat tactccttcc caggtcaact
     9181 ccttagaaga agtcaaaata agttttaaag tcaaaaatac cggaagtcga gagggagatg
     9241 aagtagtgca actgtatatc cacgaccggg tagcctcagt ggagcgaccg gtaaaggagc
     9301 tcaaaggatt taagcgcctt actctccagc ctggtgaaga aaaagaggtt attttcactc
     9361 ttttccctga gcaattagct ttttacgatg agtttatgag gctaatagtg gagccagggg
     9421 tttttgaggt tatgataggc agttcttcag aagatatccg gctttcagga gaatttgaag
     9481 ttttaaaaga aaaagtgatt accaagtata ggaaattcag tagcgaagtt atagtatccc
     9541 gtaaaactta ttaaactgtt aaaatcttta aaaagcaaat tacatcggaa gctaaaaatg
     9601 gaaaaagcaa gggatactat aacttcttta gttttttcag gtttttattt tcttaggggg
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcaagggat actataactt
     9781 ctttagtttt ttcaggtttt tattttctta gggggttata gggggttttt ccctatactg
     9841 ggaaatcttt tcttcccagt agttttgatt ttcagtcttt taggtttttg tgagtttttg
     9901 tgtgtctggg ggttataggg ggtcttttcc ccctatagtt ggtattgtct gtggcaatct
     9961 ccccaaaggt caatcgcgag cctcgtggtt tagtttagct tggcgtggca atcgcgtttt
    10021 tcctattaga gagaatctaa aaaccagatc gccacgccgc tggagtgttt tctctccagc
    10081 gtaattgggt ttagtatttt acatctcttt tgctttaagt ttgcgccgca ggctgtattg
    10141 cggttaacta taataataaa ataggaggaa actcatgaaa tttcaaggtt tacatcatgt
    10201 agctatcaaa accaacgatc tcgacaaaac tcttgatttt tattgcggca ttttagggtt
    10261 ggaagtggaa gagcgatttt atgatgcagg agagcaatcg gatattgttt tcctctcttt
    10321 aggaaacact ctactggagg tcatcgctcc ccatcggcca gaagaaaaag aactcaactt
    10381 ctctacctgt tttcacctgg ccttagccac ttccaatgta gaagaaacct acgaaaagtt
    10441 gaaagaaaaa gggataaatt tcactctccc cctcacagaa agcggtggat tcaagtatgc
    10501 ctatttttct ggaccaatgg gagaaactct ggaaatagtg gaaagaataa aagctaagta
    10561 aagagccctc ttttaaggaa agagagaaga gcttctcttt ctgcctctgg aattctctct
    10621 tcttctatgg tcatgccata gcgaggagaa agaagaactt gatggacggg ggtggggtcg
    10681 tatcctcccc accccgcaat atatgaccat ctcttccctg aaatctcgct gaaccacacc
    10741 cgtgcgtcat agcgggagta aatctcttct gctattattt ccagcttctc ttctacagga
    10801 agttccttaa tcttttcttc tatttgctca agccatttct caaactcgca ggtctctttc
    10861 accttttttt atcctctgta aatattcttc tactttaggc tttattccat ccttaaggct
    10921 ggataggtgc tcttggataa ctttttctcc tatctcttta gtttgaggag aggcataatc
    10981 ttccagatat tcctggaaag tcaaaatggc gttaggaatg cagaaatagt ggattttacc
    11041 ctttttagct atatccataa agtacttccc cgttcttcca caacgataat cagcagtgca
    11101 aaaagaagtt atatacccca tttgggctaa ctcccgaatt acttcgtcta aagaacgagg
    11161 gtctccaatt tgaaattgtt gacgctctaa ttcctgctca ttcgagttat cgctataagc
    11221 tccaatacca atgcgagtgg aggcatcaat ctgggtgcac cccactggaa tcacatcccg
    11281 gcggatgtga gctggttctc gagcagtgag tatcattcca gtgtaaggga cggaaagtct
    11341 aataacagtc accaggcgct taaagtcccg gtcggatacc cgatatttag tttcttccac
    11401 taaaggggtg ttcactgctg gctctaagcg aggaaaagat atagtatggg ggccaactcc
    11461 agcaaagtgt ttttctaaat caatggtgtg atagagaagc cccatcacct caaacttcca
    11521 atcatataag ccaaagagag ctccaatagc cacatcatca atccctgctt cttgagctcg
    11581 atggagagca tacaacctcc attgatagtt ggattttatt cctgaggggt gaagctgtcg
    11641 ataggtttca tgatgataag tttcctgaaa aacctgaaaa gtcccgattc ccacttcttt
    11701 caaaatctta taccgggata tgtccatagg agcagcattg acatttactc ttctaatttc
    11761 tccattcccc actttagtat cataaatggt ttgaatggtg ctcgcaatat aatcagcatc
    11821 agagagaggg tgctctccgt agaccactac caatctctta tgtcctaagg acaccaaggt
    11881 ttcagtttct cttcgtactt cttctaaagt caagcgatgt cttttctgta gattattatc
    11941 ccgacggaaa ccacagtaca gacaactgtt aacacagaaa ttgctacagt aaagaggagc
    12001 gaaagtcact atcctgtccc cataaacact tctttttatc tctcctcctg cgcggtacat
    12061 ttcctcccac agatcttcat cttctacatt taataatgta gcagtttctt cgggctccag
    12121 gcgctcaaga gatagtgatt tagcaatgat gtctcgcact ttttggggct cgggattacg
    12181 gtttttctgg agaagctctt ctatttcttc atcattaata aagtcctttc ccccttctaa
    12241 aaacttttca tcctcctctt ccttgataat gttacccatc cactctttga cgctcatttt
    12301 cttcgccttc ctttcccaaa aaaataatgc ccggccctac ggttgaggta gaaccaggca
    12361 ttaaatattt gctacccttg gtttcagaaa atcttcctcg ccgcaggttc cctaaattac
    12421 aatctaaaca atataaccaa acaaatacta tataaaatcc cctgaaatgt caaataaagt
    12481 tcaatttttg tagagcctca ataaattatt tcatcatatt ctctatagtc ccctctaaaa
    12541 cctcttagtt gtatccccgt tcccgtgaag caacctgtga ttttggatgc ctgattaaat
    12601 cttctcacca cccctcgctt ctttcttttc ctctctgggg gagagggtca gggtgaggtg
    12661 tcctcaagtt cgtcacccct gaatgcctct atcaggggtc catggttttt aaaaccaaag
    12721 aaaaagcaaa aacgtggatg cctgataaaa gatttcaggc atgacggaaa
//
LOCUS       DAOPSS010000035        12713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4159_length_12713_cov_9.499210, whole genome shotgun sequence.
ACCESSION   DAOPSS010000035 DAOPSS010000000
VERSION     DAOPSS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12713
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4159_length_12713_cov_9.499210"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..75)
                     /locus_tag="PKN31_03450"
     tRNA            complement(1..75)
                     /locus_tag="PKN31_03450"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Val,seq:tac)
     gene            complement(116..1204)
                     /gene="serC"
                     /locus_tag="PKN31_03455"
     CDS             complement(116..1204)
                     /gene="serC"
                     /locus_tag="PKN31_03455"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOA99019.1"
                     /translation="MSEKRVFVFNPGPAALPLPVLQQTQEEMLNFRGTGMSILEVSHR
                     SQEFEEVLNSAVERIKRLLGLDERFHVLFMQGGASLQFAMIPMNCADNKKPVAYVDTG
                     YWATRAIKEAQNIVDDVAVVASSKDKNYIYIPKNIVLEKDFSYLHITANNTIKGTQWW
                     KFPETGNIPLVADMSSDIFSRVFDPNPFGIIYAGAQKNAGPAGVTIVIVREDMLERVP
                     QNLPTMLKYSTFVESNSLYNTPPCFAVYVVELVTRWLEETIGGIAKMEEINSKKARLI
                     YDYIDSQDFYKNPVQPEDRSWMNVIFTLPSLELESSFIKEATQEGLIGLKGHRSVGGC
                     RASLYNAVTLEAVEVLVDFMKEFVRRNG"
     gene            complement(1746..2987)
                     /gene="tyrS"
                     /locus_tag="PKN31_03460"
     CDS             complement(1746..2987)
                     /gene="tyrS"
                     /locus_tag="PKN31_03460"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOA99020.1"
                     /translation="MKLSQEARLNKDIALIERGVEEIIGIEELKKKLLRFYQTGKPLV
                     VKEGFDPSAPDIHLGHTVTLRKLRQFQDLGHQVVFLVGDFTGRIGDPTGKSETRRQLT
                     EEEVRQNAETYTQQISKILDLQKIIIEFNSKWLSKLTLADLIEITSRVTVARMLERED
                     FAKRLKEGKPIGLHEFLYPVMQGYDSIALQADVELGGRDQLFNLLVGRDLQREFGQEP
                     QVVITMPLLEGTDGVEKMSKSLGNYIGIDEPPFTMFGKIMSIPDSLIEKYFLLLTDVP
                     MEEIRRMKAEYEKGELHPRDWKKRLAFELVRMYHGEEKALLSQEDFERAFSHKEMPEE
                     AQEIVISSSILKEGKIWILKLLQKTGVSSSNTELRRLIEQGGVYFNGERIQDVNTDLP
                     IKDGDYLKVGKKGFFRIKLED"
     gene            complement(2987..3481)
                     /locus_tag="PKN31_03465"
     CDS             complement(2987..3481)
                     /locus_tag="PKN31_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOA99021.1"
                     /translation="MDKSQDREKIKKISRSALIAALYVLLTILPPFYSFSYGPIQVRV
                     AECLTVLPFLFPETIWGLGLGCFLANLIGGLGFYDFFLGTLFTIIAGYLTSRVPYSFL
                     APLPPVLVNGFGVSMYLSFWFKVPYFYSVFYVMVGEAVATYGIGYPLLTFLTTKLKYH
                     RKEY"
     gene            3558..5468
                     /locus_tag="PKN31_03470"
     CDS             3558..5468
                     /locus_tag="PKN31_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HOA99022.1"
                     /translation="MKKKKNSSPLWIFLSGLLAGGVVWFLLFAFVLSDLKTVSNQLDD
                     FSPPLTTRIYDIEGRLLAELFTENREYVELSQVPDLLKKAFIASEDQNFYRHPGIDPG
                     GIIRAALQNLINMDIVEGASTITQQLSRNLFLSHQRTWKRKIQEMFLAFTLEKKYTKN
                     EILEAYLNQIYFGHGAYGVKAAARIYFGKNLEELNLAEIAMLTGVARSPGNFSPYVDL
                     ATAQRQQRRVLRRMKEMRFISQEHLEEALSTPIVLSGREKNRSIAPYFVDYLLKELQK
                     SYDEETIFGGGLKIYTTIDLDIQKIANQALEESEYQGAILCIEPQTGYIKAMVGGRDY
                     DESKFNRAVQAYRQPGSTFKPFIYSAALDSGFTPSTILVDEPLIFPNGWKPQNYEKTF
                     NGPITLREALEKSVNIIGIKLLQASGVDKVISYARKMGITSELRRDLSLALGTSEVNL
                     LELTNAYCAFANRGLVPEPIAILRIENFSGEVLYEGQRILRRALSEDTAYIMARLLQG
                     VIERGTAKAARIDRPAGGKTGSTEDFIDAWFVGFTPDLVCGIYLGNDDRTPLGPRKTG
                     GVIAAPLFSKIMKEAHKDIPVHDFYPSPGVREVKVCMRSGKLPSPGCNTVTVPFKAGT
                     EPREWCSGCTGD"
     gene            complement(5465..7825)
                     /locus_tag="PKN31_03475"
     CDS             complement(5465..7825)
                     /locus_tag="PKN31_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOA99023.1"
                     /translation="MGIIELSPLVLETFDFDRIVERLVSFCSSSPAKEMARSITPFSP
                     EEVHLKTQEAREALDMVQFDGSLSLRGLPDLRSLFPRIEVVDASLSLENLIEILHFFR
                     LSLEIRGEAKERKIKEKYPSLSPHFDRIKDFRPLVKKMEKVISKEGYILDTASDRLWE
                     LRKRIKFLQNRIVKELEEMLNSSQLAPLLQDRIYTQRRGRYVIPLKIQHRNAIDGLII
                     DHSSSGSTVFIEPARILELDNELELNRLQEEEEIARLLRELTAELRPFLEEIKETYET
                     LVYLDLLQAKANLGNSWRGRVPQWGERLIIRGGHHPLLGEKAVPFDLELSPDKSILVI
                     SGPNAGGKTVLLKSVALIVFLTFCGIPPPLEEGSSLPLYNYLFVDIGDHQDLENELST
                     FTYRLSSLKKALQEAESPALFLIDELGGGTDPAEGSALAIAILQYLQKRGITTLVTTH
                     LPAVKQYAFREEEAVPASLAFDPQTLEPTYQLVVGEIGPSYGIHIAERVGIPPEIIQT
                     ALTIVDKKEWEFNQLLLSLSQRREDLNRLVEEYESKLRELSRKEEELRRKEKELEKKR
                     EKIEREEREEFADYLRKTRREISQLVGKLRKEERLNQEEYQKLRKRLREEEKRWEELA
                     GDAMVSSPEENWQEGEIATIDFLRQTGKIISLDFKKKEALVEVEGRKVKTSFSHLEKM
                     EKEENEENDFSSSTAINYSPLPRVKNQVDIRALSREEAREELERYFDQVILAGFQTVH
                     IIHGKGEGVLREVTHQFLRDHPYVESFRLGYPEEGGIGVTVVTFKS"
     gene            complement(7813..8352)
                     /locus_tag="PKN31_03480"
     CDS             complement(7813..8352)
                     /locus_tag="PKN31_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOA99024.1"
                     /translation="MTIDWYSILGLVILGINIIRSAFRGFSKESLGLVGFLGGLVIGL
                     QYYPAIGGFFAHLSGKYYVWFSPLGFLLLFLPLVLVSSWAGVRFRKVFEKLDITWVDS
                     ILGSFAGIAKGILWVLLLTLFILNLSFFPMVNQSVYRSSLYLRLIQPLFIYVYEWVLQ
                     VPHLQFLQGILQKGIGWVL"
     gene            complement(8349..10700)
                     /gene="pheT"
                     /locus_tag="PKN31_03485"
     CDS             complement(8349..10700)
                     /gene="pheT"
                     /locus_tag="PKN31_03485"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOA99025.1"
                     /translation="MRISYNILKKFLPDLDIPQEELAPLLTEQGLVVEKIWRREELFP
                     FPLVVATLGRIEKGMLYSEGEIHFRGQVWRVKVNNWGMPEEGSKIIVGMEEETPHFLP
                     WGGEKKEEIIDFLVTLPFPAEEGEEVSSLLLGDDIVLDVEVTANRGDCLSSLGLAREV
                     GAKLGLKWQIPPVSYEEADALSDFSLENQAPDLCPYYTGKYIRRLTVRPSPWWVIKEL
                     CFLGLRPVNNVVDFTNLVMMEVGQPLHAFDASVIKGQKVVVRRAREGEKLITLDGIER
                     ELSSGMLVIADAERPIGLAGVMGGKESEITSFTSEVFLEAAIFDRVAVRKTAKALGLR
                     TEASARFERGVDPERVTYASGRVLQLLGKDGEPASGWFIAGTPPREEREVTFSPEEIE
                     KTLGCSIKKEEIIKILAGLGFEVKSLPGEETIRAKVPSWRSDIYLPVDIAEEVGRIHG
                     YDKIISRLPQFSFDPGNQNSSTLKEEKLRRHLVDKGLKEIVSFPLISPGLVELWGAKE
                     EQVDIKNPLSQEQSYLRPCLLLSALEVLKINVTRGRNDWGLFEIGKVFRRGLNTPFQE
                     EKRLFMALTGYSLPPLWHKNGEVDIFLLKGLIEELLEIAGFDRGEVRFESGGGLALEP
                     SCSFRVEVSGKEAGRGGLLSPSFIRELGFWGTIYVAEFDLDLLTTGEEKAFQLKEVVT
                     FPSVARDVSILVDEATKWGEIEEMVRRTVSREKMEVESIELFDLFQGGNVPQGKKNFS
                     FRVTFRSPARTLNDEEVDGWVQKIKADLKEKRGLLLREELLNF"
     gene            complement(10718..11728)
                     /gene="pheS"
                     /locus_tag="PKN31_03490"
     CDS             complement(10718..11728)
                     /gene="pheS"
                     /locus_tag="PKN31_03490"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOA99026.1"
                     /translation="MQELEEYWESFQEEIQKASTLSDWNYLKGKYVGKKSRWGEFLAK
                     LKELSPEEKRVWGKKINQLKKEMEELLEERYQELLSTEEKSEVDITLPGKKGWSGSFH
                     PVLLTRDWILTIFQSLGFRVEEGPEIETEFYNFEALNIPPDHPARDDQDSFYLAPGYL
                     LRTHTSPAQIRVMEKLKPPFKVVVPGKCYRRDAFDATHSPMFHQIEGLVVARDISMGD
                     LKGTLETFVRRFFGEERKIRFRPGYFPFTEPSAEVDVSCGVCGGKGCRSCGYSGWLEI
                     LGAGLVHPQVFTHAGYDSQDVRGFAFGLGVERITMLKFGIKDIRWFWENDVQFLEQFN
                     IF"
     gene            complement(11814..12167)
                     /gene="rplT"
                     /locus_tag="PKN31_03495"
     CDS             complement(11814..12167)
                     /gene="rplT"
                     /locus_tag="PKN31_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOA99027.1"
                     /translation="MPRVKNSVASRERRKKTMKSAKGYRGARSRSYRRAKEQVMRSQA
                     YAYRDRRNRKRDFRRLWITRINAAAREEGMNYSKFMGALRKAGVNLDRKSLSQLAITD
                     QEAFQKLVEIAKSQL"
     gene            complement(12183..12380)
                     /gene="rpmI"
                     /locus_tag="PKN31_03500"
     CDS             complement(12183..12380)
                     /gene="rpmI"
                     /locus_tag="PKN31_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOA99028.1"
                     /translation="MPKMKSHRGAAKRFKVTASGKIKRQQAGKSHLLAKKDRNRKRRL
                     RKPVTLDKVEEKRVRKLLIRA"
     gene            complement(12392..>12713)
                     /gene="infC"
                     /locus_tag="PKN31_03505"
     CDS             complement(12392..>12713)
                     /gene="infC"
                     /locus_tag="PKN31_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOA99029.1"
                     /translation="KFRYEKEKKIKLSRKKQKTSELKQLNLRPKIDQHDYEVKLRNAL
                     RFLEDGDRVKITVRFRGREAAFRDRGEELLKRMIADLEEVGKVEQDIKYEGPHLTITL
                     APRK"
BASE COUNT         3716 a         3044 c         2287 g         3666 t
ORIGIN      
        1 tggtgggcga aggaggactt gaacctccga ccccttgctt gtaaggcaag tgctctccct
       61 ctgagctatt cgccccccgc ggaagattat accacaaaac gtttccaaaa actatctatc
      121 catttcgtcg aacaaattcc ttcataaaat ctaccagcac ctctaccgcc tcgagagtaa
      181 cagcattata aagagatgca cggcaccctc ccaccgagcg gtgaccttta agaccgatta
      241 acccctcttg agtagcttcc ttaataaaac tactttctaa ttctaaagaa ggcagagtaa
      301 atataacatt catccaggaa cgatcttccg gctgcactgg atttttatag aaatcctgac
      361 tgtctatata gtcataaatc aaacgagcct ttttgctatt tatttcttcc attttagcaa
      421 tgccccctat agtttcttcc agccaccgag tgaccaattc tactacataa accgcaaaac
      481 aaggaggagt gttgtaaaga gagttacttt ctacaaaagt ggaatatttc agcatggtgg
      541 gtaaattctg cggaactcgc tccagcatat cttccctaac aataactatg gtcactcctg
      601 caggaccggc atttttctga gctccggcat aaataatccc gaaagggttg gggtcgaaaa
      661 ctctgctaaa aatatcggat gacatatcgg caaccagagg aatatttcca gtttccggaa
      721 atttccacca ttgagtgcct tttatggtgt tgttggcagt aatgtgaaga taagaaaaat
      781 ctttctccag cactatattt ttaggaatat aaatataatt tttatctttg gaagaagcca
      841 ctactgctac atcatccact atattctgtg cttctttaat tgctcttgtt gcccagtagc
      901 cagtgtcaac ataagcgaca ggctttttgt tatctgcaca attcatagga atcatggcga
      961 attgcaagct tgctcctccc tgcataaaaa gcacatggaa acgctcatca aggccaagga
     1021 gtctttttat cctctctaca gcactattta aaacttcttc aaattcctgg gagcgatgac
     1081 tcacttccag aatggacatc cctgttcccc gaaagttcaa catctcttcc tgggtttgtt
     1141 gtagaacagg tagcggtaaa gccgccggac cggggttaaa aacaaaaact cttttctcgc
     1201 tcataagctc ctcaccttct aagtttatag ttagtcttct tttcaggcct ttgcccggtt
     1261 ataaatatcc ctaattgtaa cagaattttt cctctctgat aagtaatcaa atcaacaaga
     1321 aacattataa ttaaagaaat taagatgaaa aaagcctgat ttaaaattgg tcatcctcgt
     1381 ctttttctac tccattattt attatctccg ctttcactct atcgtcagga aataaaagct
     1441 atcaggacgg atataagatt ataattataa tattccaaaa tatagatagc ttgagaggga
     1501 gagtgcctcc ttctcctctt cctcctcaat agaggaaata tctagagaga aggaatctgt
     1561 ggcatagaat taaaaagaca aaaaaatcta aaaaaattac tgagggagaa actgtttctc
     1621 cctcagtgct cacaaaaaca actgacagga ctcgccatgg gaaagaatag cgagactcgc
     1681 aacaacagat gaaagaaaaa ggttagatga gagtgattgt taagatatca ccacttactg
     1741 caaaatcaat cttccaattt aatccggaaa aagccttttt tgcctacttt taagtaatca
     1801 ccatctttta ttggaaggtc agtattcaca tcctgaattc tttccccatt aaagtatact
     1861 ccaccctgtt cgataagcct ccgcagttca gtgttggaag aggaaacgcc agttttctgg
     1921 agaagtttta aaatccagat ttttccctct ttaagaatag aagaggaaat tactatttcc
     1981 tgcgcctcct cgggcatttc tttatggctg aaagctcgtt caaaatcttc ttgtgagaga
     2041 agagcctttt cttctccgtg atacatccga accagctcaa aagctaatct ttttttccaa
     2101 tcccggggat gaagctcacc tttttcatat tcagctttca ttctccgaat ttcttccatc
     2161 ggaacatcag tcaatagaag aaaatacttt tcaatcagag agtcaggaat ggacataatc
     2221 ttaccaaaca tagtaaaagg aggctcatct atgccaatgt aattacccag gctcttgctc
     2281 attttctcta ccccatcagt tccttctaaa agaggcatag ttattaccac ctgaggttcc
     2341 tggccaaatt ctctttgcaa atcccgaccc actaaaagat taaagagctg gtctcttccc
     2401 cctaactcta catcagcttg aagggcaata gagtcataac cctgcatcac cgggtaaaga
     2461 aactcatgca atcctatcgg ttttccctct ttcagtcttt tagcaaaatc ctctcgttcc
     2521 agcatccggg ccacggtaac acgagaagta atctcaatca agtcagcaag ggttaacttg
     2581 ctcaaccact tgctattgaa ttctatgata attttttgta aatcaagaat tttagaaatc
     2641 tgctgagtat aggtttcggc attttgcctg acttcttctt cagtcagttg ccgacgggtt
     2701 tcacttttac cggtagggtc tccaattctt cccgtaaaat caccaactaa aaaaactact
     2761 tgatgcccta aatcctgaaa ttgacgcaat ttccttaaag taacagtatg ccccagatga
     2821 atatcaggag cactggggtc aaaaccttct tttaccacca ggggcttacc ggtctggtaa
     2881 aaacgtaata acttcttctt caattcttct attccaatga tttcttctac tcctcgctca
     2941 atgagcgcaa tgtctttgtt taatctcgct tcctgggaca atttcatcaa tactcctttc
     3001 tatgatattt taatttagtt gtcagaaatg tcaaaagagg atacccgata ccataggtag
     3061 ctacggcttc tcctaccata acataaaaca ccgagtaaaa ataaggaact ttaaaccaga
     3121 aagaaaggta catagataca ccaaatccat tcactaaaac aggaggtaaa ggcgccagaa
     3181 aagaataagg aactcgagat gttaggtatc cagctataat ggtaaaaaga gttcctaaaa
     3241 agaagtcata aaaacctaaa cccccgatta aattggctaa aaaacaacct aaacccagac
     3301 cccagatagt ttcgggaaaa agaaaaggta gaacagtcaa acactctgca actctcacct
     3361 ggatgggacc gtaggaaaaa gaataaaaag ggggaagaat ggttaataaa acataaaggg
     3421 cagctattaa agctgaccga gagattttct tgattttttc tctgtcttga cttttatcca
     3481 taaagtgatt ttattatata tattatatta aataaagaaa ggatttcgca tcaatattat
     3541 ataaataggt gaaaaccatg aaaaagaaaa aaaacagctc tcccttatgg atttttctct
     3601 ctggtctttt agcaggggga gtggtgtggt ttttactctt tgcttttgtc ttgagtgatt
     3661 taaaaacagt ctccaatcag ttggatgatt tttctccccc tctcaccact cgtatatatg
     3721 acattgaggg acgcctttta gcagagcttt ttactgaaaa cagagaatat gttgaacttt
     3781 ctcaggtccc ggatttatta aaaaaagcct ttattgccag tgaggatcag aatttttatc
     3841 gtcaccccgg tatagaccct ggaggtatta tcagagcagc tttacaaaac ttaataaata
     3901 tggacatagt agaaggggca agcaccataa ctcaacagct ttctcgaaat ctgtttcttt
     3961 cccatcagcg gacctggaaa aggaaaatac aagagatgtt tttagctttt accctggaaa
     4021 aaaaatacac taaaaatgaa atattagaag cttatctcaa ccagatttac tttgggcatg
     4081 gtgcctacgg ggtaaaagct gctgctagaa tctattttgg aaaaaactta gaagagctca
     4141 acctggcaga aatagccatg ttaactggag tagctcgttc tccaggaaat ttttcccctt
     4201 acgtagattt agctactgct cagagacaac agagaagagt gctgaggaga atgaaagaaa
     4261 tgaggtttat ttcccaagaa catttagaag aagctctctc cacccctata gttctttctg
     4321 gacgggagaa aaatcgctcc attgctcctt actttgtaga ttacctcctt aaagaattgc
     4381 aaaaaagtta tgatgaagaa actatatttg gtggagggct aaaaatatat accaccatag
     4441 atttagacat tcaaaaaata gctaaccagg ctttagaaga aagtgaatat caaggagcca
     4501 ttctgtgcat cgaacctcaa actggctaca tcaaagctat ggtgggagga agagattatg
     4561 acgaaagcaa atttaaccgg gcagtgcagg cttaccgtca gcccggctct actttcaaac
     4621 cctttatcta ttctgctgcc ctagatagtg gttttacacc cagtacaatt ttagtagacg
     4681 aacctctaat tttccccaat ggctggaaac ctcaaaacta tgaaaaaact ttcaatggcc
     4741 ctattacctt aagagaagct ttagaaaaat cagtaaatat aatcggcata aaacttctcc
     4801 aggcttccgg agtggataaa gtaataagtt atgctcgtaa aatgggaatc acaagcgaac
     4861 ttcgccgaga tttatcccta gccttaggaa cttcagaggt aaatctttta gagctaacca
     4921 atgcttattg cgcttttgcc aacagaggcc tggttcctga acccatcgcc attttaagga
     4981 tagagaattt ttccggagaa gttctctatg agggtcaaag gattttacgc agagctctat
     5041 cagaagatac tgcctacatc atggctcgtc tcctgcaagg agtaatagag cgagggacag
     5101 caaaagcggc cagaatagac cggccagcag gaggcaaaac tggaagcact gaagatttca
     5161 ttgatgcctg gtttgtgggt tttactcctg atttagtctg cggaatatat ctaggtaacg
     5221 atgaccgcac tcccttaggt cctcgaaaaa ccggaggagt aatagctgct cccttgtttt
     5281 ctaagataat gaaggaggct cacaaagata ttccggttca tgatttttac ccttctccgg
     5341 gagtaagaga agtcaaggta tgcatgagaa gcgggaagtt gccatcccct ggttgcaata
     5401 cagttactgt tccttttaaa gcgggaacag aacccagaga atggtgtagc gggtgtacag
     5461 gagattaaga tttaaaggta actacggtaa ctccaattcc tccttcttct ggatacccca
     5521 aacggaagct ttccacatag gggtggtcgc gtaaaaactg atgagtgact tcacggagaa
     5581 ccccctctcc ttttccatga attatatgaa cagtctggaa tccagcgaga attacctggt
     5641 caaaatatcg ctctaactcc tcccgggcct cctcccgact gagcgctctt atgtctacct
     5701 ggtttttaac ccggggcaag ggggaataat taatagccgt ggaggaagag aaatcatttt
     5761 cttcgttttc ttctttttcc attttctcaa ggtgagaaaa agaagtttta acctttcttc
     5821 cttctacttc caccagcgcc tctttctttt tgaaatccag agaaataatt ttaccggttt
     5881 gcctgagaaa atctattgtg gctatttctc cctcctgcca gttttcctca ggagaagaaa
     5941 ccattgcatc tccagctaat tcttcccacc gcttttcctc ttctctcaaa cgctttctta
     6001 acttttgata ttcttcctgg ttcagtcttt cttctttcct cagctttcct accagctggg
     6061 atatttctct ccgggtcttt cgaagatagt cagcaaactc ttctctttcc tctctttcta
     6121 ttttctccct tttcttctcc agttcttttt ctttccgcct caattcttcc tctttccggg
     6181 aaagctctct taacttactt tcatactcct ctactaatct attcaaatcc tcccggcgct
     6241 gggagagaga aagaagaagc tggttgaatt cccattcttt tttatctacg atagtgagag
     6301 cagtttgaat tatttccgga ggaataccca ctctttcggc aatatgaatt ccatagctgg
     6361 ggccaatttc tcccaccacc agctgataag taggctctaa agtttgagga tcgaacgcca
     6421 gagatgccgg gacagcctcc tcttctcgaa aagcatattg tttcactgca ggaagatgag
     6481 tggtcacgag agtggtaatc cctcttttct ggagatactg aagaatagct atagccagag
     6541 cagaaccttc cgccgggtct gttcctcctc ccaattcatc aattaaaaac agtgccggac
     6601 tctcggcttc ctgcagcgct ttttttaagc tactcaaacg ataggtaaaa gtgcttaatt
     6661 cgttttccag gtcttgatgg tcaccgatat ctacaaaaag gtaattataa agaggaagag
     6721 atgaaccttc ctctaaaggc gggggaatgc cacagaaagt caggaaaact atcaaagcca
     6781 cgcttttcaa aagaactgtt ttgcctccag cattaggccc gcttatcact aaaatagatt
     6841 tatccggaga taattccaaa tcaaaaggaa ccgctttttc tcccagtaaa ggatggtgac
     6901 ccccacgaat tatcagcctt tccccccact gaggtactct ccctctccac gagttaccca
     6961 aattagcttt agcttgcaat aaatccagat agactaaagt ctcataagtc tcctttattt
     7021 cttccaaaaa agggcgaagc tcagcagtta actcacggag taaacgagct atttcctctt
     7081 cttcctgcaa ccgattcaat tccagttcgt tgtccagttc cagaatcctg gctggctcga
     7141 tgaaaaccgt tgagccactg gaagaatgat ctattatcaa accatctata gcattgcgat
     7201 gctgaatttt caaaggaatg acgtaccgtc cccgtctctg ggtgtaaatc ctatcttgaa
     7261 gaagaggagc cagttgagag ctgttaagca tttcttctaa ttctttcaca atcctgttct
     7321 gaagaaattt tattctcttc ctcagctccc agagtctgtc gctcgccgta tctaaaatat
     7381 agccctcttt agaaataact ttctccatct tctttaccag aggacgaaaa tcttttattc
     7441 tgtcaaagtg tggagaaagg gaaggatatt tctcctttat ttttcgctct tttgcttctc
     7501 cccgaatttc cagactcaat cggaaaaaat gcaagatttc tattaaattt tccagactga
     7561 gggaggcatc caccacttct atccggggaa aaagagaacg aaggtcgggc aaccctcgaa
     7621 gactcagaga cccatcaaat tgaaccatat ccagggcttc tcgggcttcc tgggttttaa
     7681 gatgaacctc ctcaggagaa aaaggagtaa tagaacgagc catctcctta gcaggagaag
     7741 aagagcaaaa agacaccagg cgctccacga ttctgtcaaa atcaaaagtt tctaaaacca
     7801 gaggagaaag ctctataata cccatcctat tcctttttga agaattccct gtaaaaattg
     7861 gagatgagga acttgtaata cccattcata aacataaata aacagaggtt gaattaatcg
     7921 gagataaaga gaagaacgat aaacagattg attaaccatg gggaaaaagg acagattgag
     7981 gataaaaagg gtgagaagca aaacccacaa gattcctttc gctattcctg caaacgagcc
     8041 cagaattgaa tctacccagg ttatatccag tttttcaaac acttttcgga atcgcacccc
     8101 tgcccaggaa gaaaccagaa ccagcggcag gaaaagtagc aaaaaaccca gaggagagaa
     8161 ccagacataa tacttaccag ataaatgagc aaaaaaacca cctattgcag gataatactg
     8221 taaacctatc accaggccac ccaggaaacc caccaatccc agagactctt tgctaaaacc
     8281 cctaaaagca ctccttataa tattgatacc cagtattacc aggcctaaga tggaatacca
     8341 atctatggtc aaaaattcaa aagttcctcc cgcaaaagta gacctctttt ttctttgagg
     8401 tctgctttaa ttttttgcac ccatccatcc acttcctcgt cattcaaagt tctcgccggg
     8461 gaacggaaag taacccgaaa agaaaagttc ttcttaccct gaggaacatt ccctccctga
     8521 aaaaggtcaa aaagctctat gctttccacc tccatctttt cccgggacac agtgcgcctc
     8581 accatttctt ctatttctcc ccactttgtt gcctcatcta ccagaataga gacatcccgg
     8641 gccacagagg gaaaagtaac cacttctttt aactggaaag ctttttcctc tcctgtggtt
     8701 aataaatcca gatcaaattc tgctacatat attgtacccc aaaatcccag ttctcgaata
     8761 aaggaagggg ataaaaggcc tcctcttcct gcttctttac cggaaacttc tacccggaaa
     8821 gagcaagaag gttccaaagc caatcctcca ccactctcaa aacggacttc tccccggtca
     8881 aaaccagcta tttccagaag ttcctctatt aaccctttca gcaaaaaaat gtctacctct
     8941 ccatttttgt gccacagagg aggcaatgaa taaccggtta aagccataaa caatcttttc
     9001 tcttcttgaa aaggagtatt taatcctctt cgaaacactt ttcctatttc gaataatccc
     9061 cagtcatttc tccctcgagt aacgttaatt tttagaacct ccagcgcaga caaaaggaga
     9121 cagggacgga gataagattg ctcttgagaa aggggattct ttatatctac ctgctcttct
     9181 ttcgcccccc atagctctac cagcccggga ctgatcagag gaaaacttac tatctctttc
     9241 aaacccttat ctactaagtg tctcctcagt ttttcttctt taagagtgct actattctgg
     9301 ttcccggggt caaaagaaaa ttggggaaga cgagagataa ttttatcata gccatgtatc
     9361 cgacctactt cttcggcaat atctacagga agatagatat cactccgcca gctgggaacc
     9421 ttagctcgaa ttgtttcctc tccaggaaga gattttacct caaaacccag cccggccaga
     9481 atttttatta tttcttcctt ttttatagaa caacccagag tcttttctat ctcctccggg
     9541 gaaaaagtca cttccctttc ttcccgggga ggagtgcctg ctataaacca gcctgaagca
     9601 ggttctccat cttttcccag aagctgaagc acccgaccag aagcataagt tactctctcc
     9661 gggtctactc ctcgctcaaa acgagcggaa gcctcagtcc tcaaccccag agctttagct
     9721 gtttttctca ctgccacccg gtcaaaaatt gccgcttcca gaaacacttc tgaggtaaaa
     9781 gaagtaattt cgctctcttt ccctcccatt actcccgcca aacctatggg gcgctccgca
     9841 tctgctatca ccagcatccc tgaagatagc tctctctcta tcccgtcaag agtgattaat
     9901 ttctctccct ctcttgctct tctcaccact actttttgcc ctttaataac tgaggcatca
     9961 aaagcatgta aaggctggcc aacctccatc atcaccaggt tggtgaaatc taccacatta
    10021 ttgaccggcc tcaaacccag aaaacaaagt tcttttataa cccaccaggg ggaaggccga
    10081 acagtaagcc gccttatgta tttcccagtg taataaggac agaggtccgg cgcctggttt
    10141 tccaggctga aatcggaaag agcatcggct tcctcgtagc taaccggagg aatctgccac
    10201 tttaaaccca gttttgcccc cacctctcga gctaaaccca gagaagaaag gcaatctccc
    10261 cggttggctg tgacttctac atccagaact atatcatcac ctaaaagaag agaggaaact
    10321 tcttcccctt cttctgcggg aaaaggaaga gtaaccagaa aatctattat ttcttccttt
    10381 ttctctcctc cccagggtaa aaaatggggc gtttcttctt ccatacccac tattatttta
    10441 cttccttctt caggcatccc ccagttattt acttttactc tccagacctg tccccgaaaa
    10501 tgaatttctc cttctgaata aagcatccct ttttctattc ttcctaaagt ggccaccacg
    10561 agaggaaagg gaaacagttc ctccctcctc cagattttct ccactactaa tccttgctcg
    10621 gtaagaagag gagctaattc ttcctgaggt atatctaaat caggtaaaaa cttttttaga
    10681 atattatagg aaattctcat acggatattg gcctcaccta aaaaatattg aattgttcca
    10741 gaaattgcac gtcattctcc cagaaccacc ttatatcttt tatgccgaat ttaagcatgg
    10801 tgattctttc tacccccagg ccaaaggcaa accctcgcac atcttgagaa tcgtaaccag
    10861 catgggtaaa aacttgagga tgaactaaac cggctcccaa aatttctaac cacccactat
    10921 atccacaaga acgacagcct ttgcccccgc atactccaca ggaaacatcc acttctgcac
    10981 tgggttcggt aaaaggaaaa tacccgggac gaaatcttat ctttctctcc tcgccaaaaa
    11041 aacgacgcac aaaagtctcc agagtccctt ttaagtctcc catagaaata tctcgagcta
    11101 ccactaaacc ctctatttga tgaaacatag gactatgagt agcatcaaag gcatctcgac
    11161 gatagcattt tcccggaacc accactttaa aaggaggttt tagtttctcc ataacccgga
    11221 tttgagcagg agaagtatga gtgcgaagca aataaccagg agccagataa aaagaatcct
    11281 ggtcatctcg agcggggtgg tcaggaggga tattcaaggc ttcaaaattg taaaactcgg
    11341 tctctatctc tggtccttcc tccactcgaa atcccaggct ctggaagata gtcaaaatcc
    11401 aatcccgggt aagaagaaca ggatgaaaag aacctgacca tcctttcttc cccggaagag
    11461 tgatatcaac ctctgatttt tcctcagtgg agagaagctc ttgatatctt tcttccagca
    11521 attcctccat ctctttcttg agctggttaa tttttttgcc ccagactctt ttttcttccg
    11581 gagacaattc ttttagctta gccaaaaatt ctccccaccg acttttcttc cctacatatt
    11641 tacctttcaa ataattccag tcactcagag tgctggcttt ttgtatctct tcttgaaaac
    11701 tttcccagta ttcctctaat tcctgcaaga tacttcctcc attcccatag aaatagctgg
    11761 aagattgtcg atgattccca attttgaaga atgaaagagc aaaatcaaag gatttaaagc
    11821 tgggatttag ctatttccac cagcttctgg aaggcttcct ggtcagtaat ggccagttga
    11881 gagagacttt tccgatccag gtttactcct gctttacgga gagctcccat aaacttgcta
    11941 taattcattc cttcctctct tgctgctgca tttattcgcg taatccacag acggcggaaa
    12001 tctcgtttac ggtttcttcg gtctctgtaa gcgtaggctt gagacctcat tacttgctct
    12061 ttagctcttc ggtaagaacg ggaacgggct ccccgataac ctttagctga tttcatagtt
    12121 ttctttcttc tctcgcgaga agctacacta ttttttactc gaggcatagt tcattcactc
    12181 ctttaagcac ggataagtaa ctttcgcact cttttttctt ccactttatc cagagtaaca
    12241 ggttttctaa gccttctctt tctatttctg tcttttttag ccagaagatg gcttttaccc
    12301 gcctgttgtc tttttatttt accactggca gtgactttaa atcgctttgc cgcacctcga
    12361 tgggatttca tcttaggcat attcgttctc ctcacttcct gggggctaag gtaatcgtaa
    12421 gatgtggccc ctcatacttt atgtcctgtt ctactttccc cacttcctct aagtcggcta
    12481 tcatcctctt taagagctct tctcctctgt ctcgaaaagc cgcctctctt cctcgaaatc
    12541 tgaccgttat ttttacccga tcaccatctt ctaagaagcg aagcgcattt ctaagcttaa
    12601 cttcataatc atgttgatcg attttaggtc tcaaattaag ttgcttcaac tcagaagttt
    12661 tctgtttctt gcgggaaagt ttaatctttt tctccttttc atatctgaac ttt
//
LOCUS       DAOPSS010000036        12706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4163_length_12706_cov_8.734725, whole genome shotgun sequence.
ACCESSION   DAOPSS010000036 DAOPSS010000000
VERSION     DAOPSS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12706
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4163_length_12706_cov_8.734725"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(533..2488)
                     /locus_tag="PKN31_03510"
     CDS             complement(533..2488)
                     /locus_tag="PKN31_03510"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOA99030.1"
                     /translation="MNHKEADITRTSLPSFRIYQLGSEKAVEFAADELKKYLQRILGK
                     DTSVVLEKKENFNQAVKDVIWVGTSEKFHSDLGLEVQNPELDDAIAIEVEGGSGIISG
                     VNPRSVLLGVYRFLTEIGCRWVRPGLDGEILPSRESPDFTVQIKERASHRHRGICIEG
                     AVSLENVRQIIDWAPKVGFNAYFIQFREAYTFFERWYTHRHNPFKQPEPFSVEKAREY
                     TRELEEEIKKRGLLYHAVGHGWTCEPLGIPGLSWEPGDYHVPPQYVGYLAEVNGKRAL
                     WEGIPLNTNLCYSQPEVRKLIVNEVVAYLQEHPEVDFLHLWLADESNNNCECDDCQRA
                     LPSDFYLQILNELDEALTREKLNNKIVFLVYFDLLWPPRETRLKNEERFVMMFAPITR
                     TYQHPYPATMPALPLPPYNRNHLQFPTTVEGNLAFLREWQNIFSGDSFTFEYYLMWEH
                     YTDPGYYKIARLISEDIKSLKSLGLNGLVTCQVQRAFFPTGLPFYLMGKLLWNDRLIF
                     EEVAEDYFLSAFGYEGKKCYEYLKNLSRLFTPLFQEENLEEKEIYEYGEKIEKLIKEF
                     HPVIEKNARGDCMTRAQSWQYLEYHAELCSQLAKILIEKQKGDKEKGRERWEELKTFL
                     QKEEDQMQPVFDLFEYIETMERKILPR"
     gene            complement(2539..3405)
                     /locus_tag="PKN31_03515"
     CDS             complement(2539..3405)
                     /locus_tag="PKN31_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOA99031.1"
                     /translation="MNNTATPQVKKRPEEIGILPQTIMYLILFGFTILTLYPLIWMVI
                     NSFKTTQEFLINPLGLPNRWFFKNYPLAWRLANFSTLFLNSILYTGISTIGIIFFSLA
                     AAFAFAKIKSKATSFLYGGFLIGILFTIESLMIPLYLMSFTLGLTDTRIGVLIPYIGL
                     GLPIGIYLCTEFIKSLPDSVVEAARIDGASYFKIFLYIIIPMAKPVIATVAIMNALGI
                     WNEFMLINILVSRSDLLSLPVGIMKFSGALASDYGKQFAALVIGTVPTVSFYLVLRNR
                     ITQGVAAGATKG"
     gene            complement(3405..4316)
                     /locus_tag="PKN31_03520"
     CDS             complement(3405..4316)
                     /locus_tag="PKN31_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOA99032.1"
                     /translation="MVKPSRHSTKSEERRAYWLLVLPAVVIYWAVTAFPLFFSIILSL
                     TNYSGGPIFAFDFKFVGLNNYIRMFKDPYFWISLKNNAYIILVSVFGQIPLGFIFAYA
                     IFRKLVRFGDFFQTMIYLPAVISPIVVGILWQTFFSPYGPLTKIVQHFKPGWRSTFLL
                     DPKRAIIPVLFVILWMYTGTYLIIFLANLQKFEPSILEAAKIDGATEFQTLWYVILPA
                     LSGVIVTTVILAISGSLKSFNLIFAMTGGNPARRTSVLALYMYDTAFRGAPNYPMASA
                     ISMFIVMLSIILIIFTKAVENRYGGRE"
     gene            complement(4471..5793)
                     /locus_tag="PKN31_03525"
     CDS             complement(4471..5793)
                     /locus_tag="PKN31_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOA99033.1"
                     /translation="MFKKSRMISILLAAFLVVAVVSGLSAQEQITLKVLNYLDLTSPG
                     TEREIKEIWEAFEKNNPDIKIEREDLFGEPFHQKLEAYAAAGNLPDVIYMWPGARSAT
                     LHDKKLVKDLAPFLGDMADEFVPAAVDPNNQRGKYLAMIPIGITATHMLYVNQEMLND
                     LGLNMPETYDELKTMVPKLQEAGKDVVIMGAQDDWVIQSVLFSMIVGRLAGDAFIDKI
                     LAGEAKFTDEPFVSTLKFYQQLFDDGVLTPDIVMTPYGTSPALFAAGEAPFTIDGDWR
                     VGAFLTDPATGEALIPPEKQDNILLTIFPAIPDEVTPNSTSMVLATGFGMSAAIPDNS
                     PKEEAAWRLISWLVSPEVQKIRLETGAAIPSRKGITAEGLEPLVEQRLRFYEKYVGTY
                     VLDDVLPPEAYMPINVGLQEIALGMSTPEDVAAKVQKGYEEWIAGLEE"
     gene            complement(5973..7151)
                     /locus_tag="PKN31_03530"
     CDS             complement(5973..7151)
                     /locus_tag="PKN31_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOA99034.1"
                     /translation="MSRSQKSLNSTRVVRLFNVNSLLRTLYREGGCSRTKLAKVTHMS
                     PATVSRIISELVDQQIIIEKGTGKSSAGRKPTILQLNYEKIYIAGACISRDQVGLAIA
                     DLKGKILSKRYFQPYSLEPESLIKELVHELNLLVQEASINQEYILGLGLAISGIVDSE
                     QKTLVRSVNLGWREVKIGEILEKELELPVLVENDANAAALAELWFGGATEVRNFLYIK
                     TDTGVGSGIISERNLLTGPRGIAGEIGHLCFIPDGRKCRCGLHGCLETYLYAPDITRR
                     YEEKTGKHLESIFHLKEKVQEGDSVANKIVDEAAEALSIAVLFAAGLLDLDLVVIGGA
                     WGALGDDFLNLIREKTMDRWERSGIGRIVPVTGSQLGESSDLLGAVGLVIHRWLASPI
                     "
     gene            complement(7594..8772)
                     /gene="larC"
                     /locus_tag="PKN31_03535"
     CDS             complement(7594..8772)
                     /gene="larC"
                     /locus_tag="PKN31_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOA99035.1"
                     /translation="MGNFLYLDCFSGVSGDMFLSSLLDLGISPSLFISEIKKISIPFE
                     IEIEKVQKQNISATQVKIREEEHYHHRPARELIRIIQESPFPPSIKEKAQEIVWKIAE
                     VEAKIHGADPQEIYLHEVGGADTLVDVIGVLMALHMLGIEEIRSSPIPTGHGWVETAH
                     GRLPIPAPATLELLRGIPVYSGEIEGELTTPTGAALVSSLSESFGDLPPCQIEKVGYG
                     AGEKDYPHPNVLRAIMGKRESRLKEEKNLMIETNIDDMSPQVVEYLTEKLFQAGALDV
                     FITPIFMKKQRPAFQLSVIAPPFLKSKIEEIILKETTTLGVREYQVDKYFLPREEIEI
                     LTPWGKVRGKLSYWGEMVKITPEYEDCKKIAAQEKIPLLQVIQEAQREGEKIAEGKCF
                     "
     gene            complement(8773..9528)
                     /gene="larB"
                     /locus_tag="PKN31_03540"
     CDS             complement(8773..9528)
                     /gene="larB"
                     /locus_tag="PKN31_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOA99036.1"
                     /translation="MPEKWEETLKDLWERKITPEEAKQKLRNYPFEDLGFAKIDYHRG
                     IRKGFPEVIFCPGKTKEEILTIMQKLATEYPNILATRANEEIYEVVKSSLPDARYHPE
                     ARAIFLEREPREKKGKIALVSAGTSDLPIAEEAALTAEIAGSKVERIFDVGIAGLHRL
                     LPYLPKLWEANVIIAVAGMEGALPGLLAGLVNRPIIAVPTSTGYGANLRGITPLLTML
                     NSCSPGVAVVNIDNGFGAGFLAHMINQLAEGVR"
     assembly_gap    9815..9914
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10110..11303)
                     /locus_tag="PKN31_03545"
     CDS             complement(10110..11303)
                     /locus_tag="PKN31_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99037.1"
                     /translation="MKKVKIIVIIVLMVLFISVSEGKERALFIDSTSSQVFTPLRSFL
                     ERGERWEWIEISPALQGAEGLDFSELQEKCDFLFRGYERDDLLFFEVVDLKTSSKILA
                     LVRKKEDEDYMLEELKEKLAREFTFHREIKIIFVREEYGKSSIIFSDIEGRRQIPVYT
                     TEEAYIESPLLSPDTRYLIFVLDRGWGKGIYRLNLENGKVETLSVTDFKDYSPALSSR
                     GHKIAFVSERKNKQSIFQMNIDGSSQEPLIEKDNLLQWPAFSPDDRYLAYCELINGQW
                     SLKLHDLLTQEEKDFDFPLNVKQPVFASGGKRIIFVGEEGGNSDIYLLDLVNQNLTRL
                     TYDNSPKENPFPSPDNNWVAFSSQKEGNNWDIFLLDMMKGTTKRLTSSWGRETHPSFS
                     PFPVY"
     gene            11367..12290
                     /locus_tag="PKN31_03550"
     CDS             11367..12290
                     /locus_tag="PKN31_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOA99038.1"
                     /translation="MANFSIITVSREVESYGDEIAEKLSQKLKMELLGKEKFVSLLRD
                     ISSQFPTDPPEDEKERDRWIIEVGSFLSRRAAKKPFILVGRGGQKILENCPFAFHILV
                     VASQVTRLRRTMEKYQQDEVTASRILAEQDQQRERFLRQTFGIDWRDPVYYHLVVNTD
                     FYGVNEATDLILEAQDKVVTPPQPKKVEEQPLFPREDLGQRITFSHPSEEEFARVLDF
                     YGVRWLYEPRTFPLEWDEEGNLSEAFAPDFYLPDFDLFIELTTQKQKLTRKKNRKVRR
                     LRELYPQVKIRVVYARDYEYILNHLKGESNE"
     gene            12283..>12706
                     /locus_tag="PKN31_03555"
     CDS             12283..>12706
                     /locus_tag="PKN31_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOA99039.1"
                     /translation="MNERRLGNLLFGREEIEERVEEVAKNISLEYENKRPVLVAILKG
                     AVYFAVDLTRNLEIPFDLDFLALSGFKPGLLRVEIEKDTDIDLKEREVLIVEDIVDTG
                     LTLNFLTRELEKRFPKGVKVCTFLNCPARRIVEVPVHYC"
BASE COUNT         3498 a         2692 c         2667 g         3749 t
ORIGIN      
        1 tgtaaaatcg gacggactca atttataatt catttcttaa cacctccagc tttttgctgt
       61 catttatttc cagagcctaa agacttttgg tccgcctgat tttactgcta acgggggatg
      121 taaatttctg ggtggtgtgc ctcaatatct gctttttgat ttcagtattc tcataaaacc
      181 attagaaagc tgctgaaaaa atgcgtatct atctttagac ctttactttt ttatataact
      241 tatattacgt tttacatcaa tactttgcaa cctggatttt accatttaac ctgaccttaa
      301 tctagtattg tctttgcgaa aatagtagcc tcacaaaacc tgttgtaatt tctctccgaa
      361 tggaaagaaa agttgatgca aagtgctcgc aaagacatcc cggggtgtaa ttatccgaaa
      421 caatgtctta attgtgaaag atgagtagag cacggaggaa agagtatact gtggattttg
      481 cggtttgcta taatatggct tcctgtcgaa attataaaaa aagcttgcta ccttatcttg
      541 gcaatatctt tcgctccatg gtctcgatat attcaaaaag gtcgaatacc ggctgcatct
      601 gatcttcttc tttctggaga aatgttttaa gctcttccca ccgctctcta cctttttctt
      661 tatctccttt ttgcttttct atcaatattt tagccagttg agagcaaagc tcggcatggt
      721 attctaaata ttgccatgat tgagctctgg tcatgcagtc tccccgggca tttttttcga
      781 taaccggatg gaattctttg atgagctttt caatcttttc tccatattcg tatatctctt
      841 tttcttcaag attttcttct tgaaacaggg gggtgaaaag ccgggagagg tttttgagat
      901 attcatagca ttttttaccc tcataaccaa aagcgctcaa gaaatagtct tctgccacct
      961 cctcaaagat gagcctgtca ttccacaata actttcccat aaggtagaag gggagtccag
     1021 tggggaaaaa ggctctctgc acctgacagg taaccaggcc atttaagccc aggctcttca
     1081 aactttttat atcctcactg attagccggg cgattttata ataccccggg tcagtataat
     1141 gctcccacat aaggtaatac tcgaaagtaa agctatctcc agaaaaaata ttctgccact
     1201 ctcttagaaa agcgagattt ccctctacag ttgtgggaaa ctgtaaatga ttccgattat
     1261 agggcggaag aggcagagca ggcatagtgg cagggtaggg atgttgataa gtcctggtga
     1321 tgggagcaaa catcatcacg aagcgctcct cgttttttaa gcgagtctct ctgggaggcc
     1381 ataaaaggtc gaaatacacg aggaacacta ttttattatt taatttctct ctggtgaggg
     1441 cttcgtccag ctcattgaga atctggagat aaaaatcaga aggaagagcc ctctggcagt
     1501 cgtcgcattc acaattattg ttagactcat cagccagcca caaatggagg aaatcaacct
     1561 ccgggtgttc ctggaggtag gcgacaactt catttactat taattttctc acttctggct
     1621 gggaatagca aaggttggtg ttgagaggaa ttccttccca gagagcgcgt tttccattta
     1681 cttctgctaa gtagcccaca tattgaggag ggacatgata atcgccgggc tcccagctca
     1741 agcccgggat acccagtggc tcacaggtcc agccatgccc tacggcgtga tagagtaacc
     1801 ctcgtttttt tatttcctcc tccagttccc gggtgtattc ccgggctttc tctactgaga
     1861 aaggttcagg ctgtttgaag gggttatgtc gatgagtata ccaccgttcg aaaaaagtat
     1921 aagcttcccg gaactggata aagtaagcat tgaatcccac tttgggagcc cagtcaatta
     1981 tctggcggac gttttctaaa cttactgctc cctcgataca gattccccgg tggcggtgag
     2041 aggccctttc ctttatctga actgtaaaat cggggctttc ccgggagggg agtatttctc
     2101 catcgagtcc aggtcggacc catcgacaac caatctcggt taaaaaccga tatactccca
     2161 gtagtacgct acggggattc actccggaga ttataccgct accaccttct acctcgatgg
     2221 caatggcatc atctaactcc gggttctgga cttcgagacc caggtcggaa tggaattttt
     2281 cgcttgttcc cacccagatg acatccttta ctgcctgatt aaaattctct tttttttcca
     2341 gaactactga tgtatcttta cccaggatgc gctgaagata ttttttaagt tcatctgcag
     2401 cgaactctac cgctttttcg ctacctaact ggtaaattct aaaagagggt agagaggttc
     2461 ttgtaatatc tgcttctttg tggttcatct tttttcctcc tttttaaaca gtaatgaatg
     2521 ggggtgaccc ggaaattttt agcctttggt tgctccggct gctacacctt gagtaatacg
     2581 attacggaga acaaggtaaa agctaacggt gggtactgtt ccaataacca gagcagcaaa
     2641 ttgtttacca tagtcagaag ctaacgctcc tgaaaatttc ataattccta ccggcaacga
     2701 taagaggtca ctgcgggaga caagaatatt tatgagcatg aattcgttcc agattcccag
     2761 agcattcatt atggccactg tggcaatcac tggtttggcc atgggtatga tgatatagag
     2821 aaatatcttg aaatagcttg ctccatcaat tctggctgcc tctactaccg aatcaggtag
     2881 gctttttata aactcagtac aaagatagat accaatggga aggcctaacc cgatgtaggg
     2941 gatcaggacg ccaatgcgcg tatcagttag gccaagagtg aaagacatca ggtacaaggg
     3001 aatcataagt gattcgatag tgaatagaat tcctatcaga aagcctccat acaggaagga
     3061 ggtagccttt gatttgattt tagcaaaggc gaaggcagcc gctagtgaaa aaaagattat
     3121 gccgatagtc gatatgccgg tatacaggat actgttgaga aacagagtag aaaaattggc
     3181 taaacgccag gccaggggat agtttttaaa aaaccatctg ttgggtagcc ccagggggtt
     3241 tatgagaaac tcctgagtgg ttttaaaaga atttataacc atccatataa gggggtataa
     3301 ggttaatatg gtaaaaccaa aaagtattaa atacatgatt gtctggggta aaataccaat
     3361 ctcttctggt cttttcttta cttgaggtgt tgctgtattg ttcattattc cctgcctcca
     3421 taccgatttt ctactgcctt ggtaaaaata atcaatatga tgctcaacat gacgatgaac
     3481 atggaaatcg cactggccat aggatagttt ggcgcacctc ggaacgcggt gtcatacatg
     3541 tagagagcta atactgatgt tctccgagca gggttgcctc cagtcatggc gaaaataagg
     3601 ttgaagcttt tcagagagcc agaaatggca agaataacgg tagttacaat cacccctgag
     3661 agagcgggaa gtatgacgta ccagagagtc tggaattcgg tagctccgtc aattttggct
     3721 gcttccagaa tggagggttc aaatttttgt aagttagcta aaaaaataat caggtaagtt
     3781 ccagtataca tccacaatat tacaaaaagg accggtatga tagcccgctt gggatcgaga
     3841 aggaaagtgc ttcgccatcc gggcttgaaa tgctgcacta ttttggtaag cggaccataa
     3901 ggggagaaaa atgtctgcca caaaatgcca actacgatgg gtgaaatcac agctggcagg
     3961 taaatcatag tttggaagaa gtctccgaaa cggaccagct ttctaaaaat tgcataagca
     4021 aaaataaaac ccaggggtat ttgtccgaaa accgaaacta gtattatata ggcgttgttc
     4081 ttgagggaaa tccagaaata gggatctttg aacatacgga tatagttgtt gaggcctacg
     4141 aatttaaaat caaaagcaaa tattggtcca ccactgtagt tggtcagact taagataata
     4201 gaaaagaaaa gcgggaaagc cgtaactgcc cagtatataa ctacggcggg caaaactaat
     4261 agccagtatg cccggcgttc ttcactcttt gtgctatgac gtgatggttt caccaccttc
     4321 acctccgtag agtgtctgtt cagctattcc acctgaaaat gaaaggtcag tgtttcaaga
     4381 ttgatggaaa agaagcgacc ctgcatggtt gtctcttctt atggtgcagg atcgcttcag
     4441 ggtgtgtttg cttacctctt tacttttgtg ttattcctct aaccctgcta tccactcttc
     4501 ataacctttc tgaactttgg cagctacatc ttccggggtg gacattccca gtgcgatttc
     4561 ctggagtccg acattaattg gcatatatgc ctcaggtggt agaacatcat cgagcacata
     4621 agttcccaca tatttttcat agaagcgaag tcgctgctcg acgagaggtt ctaagccctc
     4681 ggcggttatt ccctttctgg agggaatagc agcaccagtt tccagacgaa tcttttgcac
     4741 ttcaggagat acgagccagg agataagcct ccaagctgct tcttccttag gagagttatc
     4801 tggtatagca gcgctcatgc cgaatccggt ggctaatacc atagaagtac tattgggagt
     4861 tacctcatca ggaatggcgg gaaaaatggt gaggaggatg ttatcttgtt tttcaggtgg
     4921 aataagtgct tcacctgtcg cagggtcagt gagaaatgct cccactctcc agtcaccatc
     4981 gattgtgaat ggtgcttcgc cagctgcaaa cagtgcaggt gatgttccat agggagtcat
     5041 aacaatgtct ggggtgagca caccatcatc aaaaagttgc tggtagaatt taagggtaga
     5101 tacaaatggt tcatcagtga atttggcctc tccagcgaga atcttatcta taaacgcatc
     5161 acccgctaag cgcccgacaa tcatgctgaa aagaacagac tgtattaccc agtcgtcctg
     5221 ggcgcccata ataactacat ccttacctgc ctcctgtaac ttgggaacca tggtttttag
     5281 ttcatcatag gtttcaggca tattaaggcc taaatcattc aacatttcct ggtttacata
     5341 aagcatatgg gtggcggtta ttcctatcgg aatcatagct aaatacttac cacgctggtt
     5401 attagggtct actgcagcgg ggacaaactc atcggccata tcacctaaga agggagcgag
     5461 gtcttttacc agttttttat catgcagtgt tgcagaacgc gcaccaggcc acatataaat
     5521 gacatcggga aggttacctg ctgcagcata ggcttccagt ttttgatgga aaggttctcc
     5581 gaataggtct tcgcgctcaa tcttgatatc agggttattt ttctcaaaag cttcccagat
     5641 ttctttaatt tctctttctg tgccgggtga ggtaaggtct agatagttga gaaccttcag
     5701 ggtaatctgt tcctgagctg atagcccgct cacaaccgca accaccagaa aagcagccag
     5761 taaaatcgaa atcattcgag atttcttgaa catatgtaca cctcctttta aactctaaaa
     5821 tcaggcacct ttgtcttcta acgaaactaa cacgatttgc ctaactccac tatcctttta
     5881 gatgctttcc tgatttctaa catcacctcc gttgtttttt tagataatca gcaaaggctg
     5941 ataaatccac tatctctatc cgggcaactc gattatatgg gagaggctaa ccagcggtga
     6001 atgacaagac ccaccgcgcc taggaggtcg ctcgactccc ccagctggga tccggttacc
     6061 ggaactatcc taccgatacc agacctttcc catctatcca tagttttttc tctgatgagg
     6121 tttaaaaagt catcgcccag cgctccccat gcacctccaa tgactactaa atccaggtca
     6181 agcaggccag cagcgaataa aacggcaata gatagtgctt ctgcggcctc atccactatt
     6241 ttatttgcta ctgagtcacc ttcctgaact ttttccttca gatgaaaaat gctttctaaa
     6301 tgtttacctg ttttctcttc atatcttctc gtaatatcag gagcatacag atacgtttct
     6361 aaacacccgt gtaagccgca ccgacatttt ctaccatctg gtataaagca cagatggcca
     6421 atttctcctg ctattcctcg gggaccggtc aacaaattgc gttcagaaat tatgcccgac
     6481 cccacaccgg tatcagtttt aatgtataaa aaatttctta cttcagtagc tccaccaaac
     6541 cacaattctg ccagtgctgc tgcgttagca tcgttttcta ctaataccgg tagttccagc
     6601 tccttttcca aaatttctcc aattttcact tcccgccagc ctaagttaac tgaacgcacc
     6661 agagtctttt gttcgctatc tacgataccc gagattgcca gtcccagccc caggatatat
     6721 tcctggttga tgctagcctc ctggaccaac agattaagtt catgcaccag ctccttaatc
     6781 agggattcag gctctaaaga gtagggctgg aaatatctct tagagagtat tttccccttg
     6841 aggtcagcga tggccaaacc cacctggtct cgggatatac atgcaccggc tatataaatc
     6901 ttttcatagt taagctgaag gatcgtcggc ttacgtcccg cactggattt acctgttcct
     6961 ttttcgataa ttatttgttg gtctaccaat tctgatatta ttctgcttac cgtggcagga
     7021 ctcatgtgag taactttggc caacttagtt cgtgagcaac ccccctcacg gtagagggtt
     7081 cttaagaggg aattaacatt gaataaacga accacacggg tgctgtttaa agatttttgg
     7141 cttctcgaca tttagtaaca ccctaatttt ttcattgtga aataatctta ctatgagtat
     7201 agttcaaaac aacggaaaaa gtcaatacct aattcctttc attgtgaaaa aatatgagga
     7261 attaaattac tccagattcg gtgataccct gtttatcaaa ttaggctact ggttttgtat
     7321 ctttgaggtg taaaatacag tcaacatgcc taatcgtcga aaggagaatc aatgatctgg
     7381 ggtaaaacgg caaatttcca tcatagtttt ctattccact aagggtagca attacgacag
     7441 gttttgcagg atactataac cgggaaggga agctaaataa taacatcaga atttaccctg
     7501 tgttcagctg ataaaagctc tcgggagtat tagatttttg taatagtcgg tggaggtaaa
     7561 tgagaattat tatagaggaa atagcaagtc aggctaaaaa catttccctt cggcgatttt
     7621 ttctccttct ctctgggctt cctgtattac ctgaagaagg ggtatttttt cctgggcggc
     7681 tatttttttg caatcttcgt attccggagt aattttaacc atttctcccc agtaagacag
     7741 tttacctctc actttacccc agggagtgag tatttctatc tcctctcgag ggaggaagta
     7801 tttatctacc tggtattctc tcactcccag agtggtagtt tcctttaaaa ttatttcttc
     7861 tattttactt ttcaggaagg gaggggcaat taccgagagc tgaaaagccg gtctttgttt
     7921 cttcataaaa atgggagtaa tgaaaacatc cagagctcct gcttgaaaaa gcttttcggt
     7981 gaggtattct acaacctgag gagacatatc gtcgatattg gtttctatca tcaggttttt
     8041 ttcttctttg aggcgacttt ctcttttccc cattatagct cgcaaaacgt tggggtgagg
     8101 atagtctttt tctcctgctc catagcctac tttttctatc tggcaagggg gtaaatcacc
     8161 aaaagattca ctcagggaag atacaagagc tgctccagtg ggagtggtta gctctccctc
     8221 tatttctcct gaataaacag gtattcctct caaaagctct aaagtagcag gagcgggtat
     8281 aggaagtcga ccgtgagcag tttcgaccca gccgtgacct gtgggaatgg gggaagagcg
     8341 aatttcctct attcctaaca tatggagcgc cattaacact cctattacat ctaccaaagt
     8401 atctgctcct cctacttcat gaaggtaaat ttcctggggg tcggctccat gaattttggc
     8461 ttctacttca gcaattttcc agacgatttc ctgagctttt tcttttatag aagggggaaa
     8521 agggctttcc tggatgatgc gaattagctc tcttgccggg cgatgatggt agtgttcttc
     8581 ttctcttatt tttacttgag tggcggagat gttctgtttc tggacttttt ctatttctat
     8641 ttcaaaaggg attgatatct ttttaatttc ggaaataaaa agagaagggg aaattcccag
     8701 atctaaaaga gagctcaaaa acatatctcc actcactcct gaaaagcaat ccagataaag
     8761 aaagttaccc aattatctca ccccttcagc taattgattt atcatatggg ctaaaaaacc
     8821 agctccaaac ccattgtcta tgtttaccac cgctaccccc ggagagcagc tattgagcat
     8881 ggtaagaaga ggagtaattc ctcttaaatt ggctccatat ccagtgctgg tagggacagc
     8941 aataataggc ctatttacca acccggcaag aagcccgggc agggctcctt ccatacctgc
     9001 cacagcgatg atgacgttgg cttcccacag tttgggaaga tagggtaaca ggcgatgcag
     9061 gccggcaatg cctacatcaa agattctttc tactttgctt cccgcaatct cagcagtaag
     9121 agcagcttct tcggctatgg gtagatcaga agttccagca ctcactaaag cgatttttcc
     9181 ctttttctct cgaggctctc gctccaaaaa tatggcccgg gcttcgggat gataacgagc
     9241 atcaggcagg gacgatttaa ccacctcata aatctcttca ttagctctgg tagctaaaat
     9301 attgggatat tccgtagcta atttttgcat tatggttagg atttcttctt tagtttttcc
     9361 cggacagaaa attacctcgg gaaatccctt gcgaatacct cgatgataat caattttagc
     9421 aaaccctaag tcttcaaagg ggtaatttcg cagtttttgc ttggcttcct caggagttat
     9481 tttcctttcc cagaggtctt ttagtgtttc ttcccatttt tcaggaatgg tattttctcc
     9541 ttttcttttt ataagtcagt aacagaatcc cgagatgcag gaagagctgt tttttctctg
     9601 attgttttat tattattcgt tttggggagg ccagccagag tctttttggt gaggttccct
     9661 tctctttttc ctattaccat atttgagagt tgactaaaaa cagggaaggc atttcccgga
     9721 ctccccacaa agctttcaaa taattaccag aacagacttc taactctcgg ggtaatgtat
     9781 actttaaagt tttgacctcc cgccagttgc aatcnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnncgccag ttgcaatcag tacatataat taaaagagct atttctatgg
     9961 ctcttttgct ttcttttaac ttttgataaa gagcaaactc cttacagtcg gggacaaaaa
    10021 atgcaaaaaa gcaatattgg aagggcagag ttctatctct tctccccgat taaacataac
    10081 tccctacaat tataatggta atctgttttt tagtaaaccg ggaagggaga aaaactggga
    10141 tgagtttctc gaccccagga agaagtgaga cgttttgttg ttcccttcat catatctaat
    10201 agaaatatat cccagttatt accctccttc tgggaagaaa aagccaccca attgttgtca
    10261 ggagagggaa agggattttc tttgggggaa ttatcgtagg tgagacgggt taagttctgg
    10321 tttactaaat ctaataaata tatgtctgaa tttccccctt cttctcccac aaaaatgatt
    10381 ctcttgcctc ctgaagcaaa aacaggttgt tttacgttta aaggaaaatc aaaatctttt
    10441 tcctcctgag ttagaaggtc atgcaatttt aatgaccact gcccattaat tagctcacaa
    10501 taagccaggt atcgatcgtc aggggaaaaa gctggccact ggaggagatt atctttttct
    10561 atcagcggct cctgggaaga gccatctata ttcatctgga aaatactctg tttatttttt
    10621 cgttcggaga caaaggctat tttatggcct cgagaagaaa gagccggaga gtaatcctta
    10681 aaatcagtca cgctcaatgt ttccactttt ccattttcta agttgagccg ataaatccct
    10741 ttcccccagc ccctgtctaa aacaaatatc aggtagcgag tatcaggaga caagagggga
    10801 ctttctatat aggcttcttc ggtggtatag acaggaattt gccttcttcc ttctatgtcg
    10861 gagaaaataa tagaactttt cccgtattct tccctcacaa agatgatttt gatttctcga
    10921 tgaaaggtaa actccctggc cagtttctct tttagctctt ctaacatata atcctcgtct
    10981 tctttttttc tcactaaagc caggattttg gaggaggtct ttaaatctac aacctcaaaa
    11041 aatagcagat cgtctctttc ataaccccgg aataaaaagt cacatttctc ttgaagctca
    11101 gagaaatcaa gaccttctgc cccctgcaaa gcaggagata tttctatcca ttcccatctt
    11161 tcacctcttt ctaagaatga gcgaagaggt gtgaagacct gagaagaagt agaatcaata
    11221 aacagagccc tttctttccc ttccgatacc gaaataaaga ggaccatcaa tacaattatt
    11281 actattattt ttactttttt catttcattg cccctttttt ctaattctct tatttgatag
    11341 tataatagaa aactggtgaa aagaaagtgg ctaatttttc tattattacc gtttctcgag
    11401 aagtggaaag ctatggagat gaaattgccg agaagctttc tcagaaactg aaaatggaat
    11461 tgctgggtaa agaaaaattt gtttctctct tgagagatat ttcttctcaa tttcccactg
    11521 atcctccaga ggatgagaaa gaacgagatc gctggattat agaagtgggc tctttccttt
    11581 cccggagggc ggcgaagaaa ccctttattc tggtgggaag aggggggcag aagattctgg
    11641 agaattgtcc ttttgctttt catattctag tggtagcctc ccaggttacc cgtttgcgcc
    11701 ggacgatgga gaaataccag caagatgagg taaccgccag tcgaattctc gctgaacaag
    11761 accagcaaag agaaagattt ttaaggcaaa ccttcgggat agactggaga gatccggttt
    11821 attatcattt agtggttaac actgatttct atggtgtaaa tgaggctacc gatttaattc
    11881 tggaagctca ggataaagtg gtgacccctc ctcagcctaa aaaggtggag gaacaacctc
    11941 tttttccccg ggaggattta ggacagagaa taactttttc tcacccttca gaggaagagt
    12001 ttgcccgggt gttagatttt tacggtgttc gctggcttta tgagccacgc acctttcccc
    12061 tggagtggga tgaggagggt aacctcagtg aagcttttgc tcctgacttt tatcttcccg
    12121 actttgacct ttttattgag ctaaccaccc aaaagcaaaa attaactcgg aagaaaaatc
    12181 gtaaggtgag aaggctgagg gagctttatc cccaggtaaa aatccgggta gtctacgctc
    12241 gggattatga gtatatttta aatcatttaa aaggagaaag taatgaatga gaggcgttta
    12301 gggaatctgc ttttcggaag agaagaaatt gaggagaggg ttgaagaagt agcaaaaaat
    12361 atttccctgg aatatgagaa taaacgaccg gtactcgtgg caattctgaa aggagcggtt
    12421 tatttcgcag tagatttaac tcgaaactta gaaatacctt ttgacctgga ttttttggct
    12481 ctttcgggat ttaaaccagg tttgttgcga gtggaaatag aaaaagacac ggatatagac
    12541 ctcaaagaaa gagaggtttt gattgtagaa gatatcgtgg acactggttt gactctcaat
    12601 ttcttgaccc gagaactgga aaagaggttt cctaaaggcg ttaaggtttg tactttcctg
    12661 aattgtccag caagaaggat tgtagaagtt cctgtacatt actgtt
//
LOCUS       DAOPSS010000037        12623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4212_length_12623_cov_7.968651, whole genome shotgun sequence.
ACCESSION   DAOPSS010000037 DAOPSS010000000
VERSION     DAOPSS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12623
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4212_length_12623_cov_7.968651"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /locus_tag="PKN31_03560"
     CDS             complement(<1..1910)
                     /locus_tag="PKN31_03560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOA99040.1"
                     /translation="MNEHLLTQELEKRETRYRTILSSVQDIVFTLDKAQRCTGVYGQW
                     LERHHLVPEDFLGRSVQDIFGEEDSLAHREASEKAFRGESVVYEWSILVNGERRYFQI
                     SLSPIKNEEGSIEEVVGVSRDITDLKEAEEFYQKLLDGIPYPASLINRERRILAQNKA
                     TESFAGSKVGDICWFSFHGTDTLSASQKLEFEKSGVLPPDAHCLYCRGDEALERQEAI
                     VEEVELKGRILETGWVPLGSDIYLHYSIDITKYKEVERELRKERDLAQKYLDTADVIF
                     LILNPQAEVTLLNRKGAEVLGYTQEELVGKNWFDVCVPTRVQEEVKNAFARLMAGENE
                     TVGHIENPVVTRSGEERLIAWHNALLRDDENCIVAVLSAGIDITESRKMEEELRYLNF
                     HDKLTGVHSRNFLEKEIERLDIATQLPISVIMVDVNGLKLVNDTYGHERGDELLRCAA
                     QVLKASCREQDVIARFGGDEFVILLPETSWKEAQKVCQKIRSKAKGVFVEEVPLSLAL
                     GLATKESREESLTEVLRRAEDEMYRQKLAETRSSRSAIINALLNTLETKSYETQLHTQ
                     RMQKVGLAIAERIQLPDTEVNRLKLAIMLHDIGKITLPEEILKKTGPLLPEEWELIKK
                     HSESGYRIVRATP"
     gene            2383..4608
                     /gene="malQ"
                     /locus_tag="PKN31_03565"
     CDS             2383..4608
                     /gene="malQ"
                     /locus_tag="PKN31_03565"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOA99041.1"
                     /translation="MFAMINGEREITPAASYKYRRREEYFSNHQSISNRGSRMMTGEI
                     QDSFYQLLLKYSRPGNKKHGMILQGEVSMENWEEKVKRLSHLSGIQLYYYDIKGKKVE
                     ASLTTLISLLQVMGIEDLSETGLEEKIREKEEEKNNPFPPVVVTSERKLRFGPLSPQK
                     VVLMVKGEKGEEWREEFNIEEHSFLEWDFPPSMEWGYYELTFLPGEKEVVHPVFLVFS
                     PGSCFLPSHKIWGIQVPVFSLATLKSQGIGDWRDLLLVEDKLKKAGGEFLGVLPLHLT
                     ENRSPHGISPYLPLDRLLWNPIYLPLEEVASFLGYEESKKYLDELSPLFSSFSPEKEI
                     DYDGVWSLKQKFLRQTFGLFWERRNEDPRFLELESFFQEERIKWASFFYAFAEVHGLN
                     WRNWPSPYRYKEKKAVEDYIQAHREEVLYHAYLQWLMEKFLSSGSFLGFDLPVGASAG
                     GSECFLEEDCFAFQASLGAPPDDFAPAGQNWGFPPPFPEKERKNGYRHFIALIRKNMQ
                     FARYLRVDHILGWQRLYWIPEGSEAREGAYVENFLPDLLGILALESMRNEVTVIGEDL
                     GTVDPLLKESLHKFQVLSTRVFYFEKDAEGFPLSPSQYPPLSLATFNTHDLPPLVSFW
                     EGEDILLREKVGMYGEEEVRVYLGEREEFCKKVLQKLSQWGLWEEGGSLWEALLRFLA
                     LTPSLIKVVGLNDVLGEKRMINLPGTVDEYPNWRLRLPIQETNLEEALCQIGRIMKGG
                     G"
     gene            4610..5407
                     /gene="mutM"
                     /locus_tag="PKN31_03570"
     CDS             4610..5407
                     /gene="mutM"
                     /locus_tag="PKN31_03570"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOA99042.1"
                     /translation="MPELPEVEVIRRELKEKIEESRILFVRVEDSQFYLPSDMILGKK
                     INKLERKGKNLFLVLEDESSIFFHLGMTGRLLFSSREEPFPYLKVLLGLSSGFLSLTD
                     QRKLGKVALLTREETEELLRQVGIDPLSPEYTEENFEKVLSGRKMRVKDFLLSQRYIS
                     GIGNIYASEILFRSSIHPEKRTNDLTPEEKKKLFITIPQVLKEAIAHQGTTIRNYVRS
                     GGEQGEFQNSLLVYGKEGERCPCCGEKIVRKKIASRSTYFCPRCQMG"
     assembly_gap    5473..5572
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7185..7676
                     /locus_tag="PKN31_03575"
     CDS             7185..7676
                     /locus_tag="PKN31_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HOA99043.1"
                     /translation="MASKKFEGRREGQPVEKILEVGMHLILDGYCQDSSIIGNLGEVH
                     RFLDEFPEEMGMTKVLPPYVFQYQSSSPLDSGISGIVIIAESHISIHTFPVRNYLSVD
                     IFSCKPFDVEEAIQKLNSYFNLVEYNYQLFDRGIEYPPDLNSSLPLVIEERLKNLEKM
                     GHT"
     gene            complement(7735..8097)
                     /locus_tag="PKN31_03580"
     CDS             complement(7735..8097)
                     /locus_tag="PKN31_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOA99044.1"
                     /translation="MRAMEEREIEGLRAQLLELNKKIKLVLKRERDTTRDYNPLSESR
                     DEADLGSATVDLALQMSSQKTLLEMLRLVEKALKKIESGNYGLCELCKREISIQRLKT
                     IPYALYCVECQSRMENKK"
     gene            8462..9220
                     /locus_tag="PKN31_03585"
     CDS             8462..9220
                     /locus_tag="PKN31_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOA99045.1"
                     /translation="MGPKEISQKLDQLLRLDTFPVGVKFYTREEDLPKKPLPFKLNIC
                     QLVCMARYQKMGHSGVPEKMVCAFGAACLGLIQTPEAILSGKAAVGAYCKDQEAAKKF
                     MANVYRLGDDGKKYAGVFISPLETFTEEPDVVVVYGNPAQMMRFIHANAYDTGEKTTA
                     DTVAEGAMCSAIGFAKGTGKPTVGFPCAGDRIFGGTQHHELVYAAPYALFRDKLVDNL
                     EFTAKGGFSVYPVPPYMAFTPEMPPAYSIKPEDL"
     gene            9711..10934
                     /locus_tag="PKN31_03590"
     CDS             9711..10934
                     /locus_tag="PKN31_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOA99046.1"
                     /translation="MSNLWVGDNLSDVKVKNRSLVLRLLKKKGPLSRIDLARITGLTQ
                     PTITNIVNDLLSANLLQEVGISDTKAGRKPILLSVNDQAFYIIAINFSRRGFSIALCD
                     LGPNILFRCDSSYSLLENTDIALLELQKEFIHILEYATQSLRLILGIGISVPGPVDAE
                     SCTILAHPTFLKHRSLDLRFYLREYDLPIFMMHDADAACLHETWSGEARETQNMVYFM
                     VGEGVGAGILIDGKIYRGHHRQGGEIGHTSVNLFGPRCVCGNYGCLESYCSIPNVLKK
                     AQEVVWFGESSYLRDISSRQGGIEFHHLLEGARMGDNTSLQILNQLGQYVGVGMVNLI
                     NLYDPEMAVIGGEVALAQEFIAGTVNQVLEERLLYREYVNPQLRYSKWGRDVTLLGAA
                     SIVLDHFIAGELGKF"
     gene            complement(10936..11268)
                     /locus_tag="PKN31_03595"
     CDS             complement(10936..11268)
                     /locus_tag="PKN31_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOA99047.1"
                     /translation="MKKRKIWWVILPLFAAAGIWVGGKYSSSRDFYSILLETQDQIIE
                     LKVTPRTEKVITLEGPLGETIVKIEGGKVWVSSSPCPDHICIEMGKIPDNGGFIACIP
                     NRIIIRKR"
     gene            complement(11303..12322)
                     /locus_tag="PKN31_03600"
     CDS             complement(11303..12322)
                     /locus_tag="PKN31_03600"
                     /inference="COORDINATES: protein motif:HMM:NF003372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HOA99048.1"
                     /translation="MYNNFLKQEVNVFILIVGGTELGTIPGLSAAGSNPEAVAYTAPA
                     DAEVLWKGKPQIIDALPVDPEGHPTPALISRAAYLEAGFPVLIVRAGTLLPPTVPYVE
                     TAASPGRDPRRGKAVEEAFSLWEKGKMLGEELSKTQNCLVIAESLPGGTTSALCVLRA
                     LGYNGMVSSAFPHNPIPLKEKVWQEVSSFQGISYGSLQGAGLKAVESCGDPMQAVAGG
                     LVEGALPECQVVLAGGTQMLAVSAVLRHQGYNRPLFVATTRYVVEDKSAHFKEIAQEL
                     GVEVYSAPLDFSHSSFPGLSDYERGFVKEGVGAGGAVWYAEKLGIPVKTIISRVEELY
                     KNFSP"
BASE COUNT         3378 a         2656 c         2783 g         3706 t
ORIGIN      
        1 ggtgtagcgc gaactatgcg gtatccagat tcagagtgtt tctttatgag ttcccattcc
       61 tctggaagaa gaggacctgt ttttttcaaa atttcctctg gaagggttat tttcccaata
      121 tcgtgcaaca tgatggctaa tttcaatcgg tttacttcag tatcgggaag ctggattctt
      181 tcagcaatgg ccagtcccac tttttgcata cgctgggtgt gcagttgagt ctcataactt
      241 ttagtctcta atgtattgag taaggcgtta atgatagcgc tgcgggaact tctggtttcg
      301 gccagtttct gccggtacat ttcgtcttcg gctcttcgga gaacctcagt aagactttct
      361 tctctgcttt ccttagtggc aaggcccaga gctaaagaaa ggggcacttc ctctacaaat
      421 accccttttg ctttacttct tattttctga cagacttttt gagcctcttt ccaggaggtt
      481 tctggtagaa gtataacaaa ctcatcaccc ccaaagcggg ctataacatc ctgttcccgg
      541 caggaagctt tcaaaacctg tgccgcacat cgcaggagtt catcaccacg ctcatgtccg
      601 taagtgtcgt tgaccagttt taacccgtta acatctacca tgatgacgct tatgggaagc
      661 tgtgttgcaa tatccagcct ttctatctct ttctccagga aatttcggct atgtacgccg
      721 gtcagtttat catggaagtt cagatagcga agttcttctt ccattttccg actttcggtg
      781 atatcgatac ctgcactgag aactgccaca atgcaatttt catcgtctct caagagagcg
      841 ttatgccagg caatcagtct ttcctctcca ctccgggtca caacggggtt ttcgatgtgt
      901 cccaccgttt cattttctcc ggccatcaat cgggcaaaag catttttcac ttcctcctgt
      961 acccgagtcg ggacgcagac atcaaaccag ttctttccca ctaattcttc ttgagtatac
     1021 cccagaactt ctgccccttt gcggtttagc aaggtcacct cagcctgagg attaagaatg
     1081 aggaaaataa catcggcggt atccaggtat ttctgggcta agtcgcgttc tttccgaagc
     1141 tctcgttcta cttccttata cttggtgata tcgatggagt aatgaagata gatatcgcta
     1201 cccagaggaa cccatccggt ttccagaatt cttcctttaa gctctacttc ctccactatg
     1261 gcctcctggc gctcgagagc ctcatctccc ctgcagtaaa ggcagtgggc atcggggggt
     1321 aggacaccac ttttttcaaa ctctaacttt tgagatgcag agagggtatc tgtcccatga
     1381 aaagaaaacc agcatatatc ccctactttt gatcctgcaa aagattctgt ggccttgttt
     1441 tgagccagaa tgcgccgctc tcgattgata agggaggcag gatagggaat accatccagt
     1501 agtttctggt aaaattcttc agcctctttg aggtctgtaa tatcgcgact gactcctact
     1561 acttcttcga tggagccttc ttcatttttg atgggagaaa gagagatctg gaaatatcgt
     1621 cgctccccgt tcacgagaat cgaccattca tacaccaccg attcaccccg gaaagccttt
     1681 tcactggcct ctcggtgagc tagggaatct tcttcaccaa aaatatcctg aacacttctt
     1741 cccagaaaat cctcggggac aagatggtgc cgctccagcc actgaccata cactccggtg
     1801 cagcgctgtg ctttatccag ggtgaagacg atatcttgaa ctgaagagag gatggttcga
     1861 tagcgtgttt cgcgtttctc gagctcctgt gtcagtagat gctcattcat cgtatctggt
     1921 agcgccatca ccgactctgg atgtttcctt gaggaccaca aggggaactg gttgaactgg
     1981 taatctggta ttcagtttta tatctggaaa tgaacacgat attaccactg accttcctgg
     2041 tcatcccgtt tttaaaaact tgctctattg gattaaaaac tctcccttga gagtgaacat
     2101 tagcgatatg aaaaacctcc tcagtaattt tatcatatga ttctctggga atctatccgg
     2161 tcaacatttc tactacagat tcctgtacat tacctgactc tccgtatcag tagaaatcac
     2221 tcatccaccg gggaatgaaa ggtgggttgc cacttttact ttctttttct cttaccacat
     2281 ccagagattt ttgtagtatc tcctcttcgg aatattgtga aatatggccg caaagaggcc
     2341 ggtgttacct tataaaaaag ggaaagttcc ctggtctatg acatgtttgc gatgataaat
     2401 ggggaaagag agattacccc agctgcttct tacaaataca gaagaagaga ggaatatttt
     2461 tctaatcacc aatcaatttc caaccggggg tctcgaatga tgacgggtga gatacaagat
     2521 agtttttatc aattactttt aaaatattct cgaccgggca ataaaaagca tggtatgata
     2581 ttgcaaggag aagtgagtat ggaaaactgg gaagaaaaag taaaaagact ttctcacctt
     2641 tcaggaattc agctatatta ctacgatatc aagggcaaaa aagtggaagc ttcccttact
     2701 acccttattt ctctcttgca agtaatggga atagaggatt tgtccgaaac agggttagaa
     2761 gagaaaataa gagaaaaaga agaagaaaaa aataacccct ttcctcctgt tgtggtaacc
     2821 agtgaaagaa aacttcgctt tggccctctt tctcctcaga aagtggtttt gatggtaaaa
     2881 ggagaaaaag gtgaagaatg gagagaagag tttaacatag aggagcattc ctttttagag
     2941 tgggattttc ctccttctat ggaatgggga tactatgagc tcacttttct tccgggagaa
     3001 aaagaagtgg tacatcctgt ttttttagtt ttttctcccg gtagttgttt tctcccttct
     3061 cataaaatat ggggcattca ggttccggtt ttttcccttg ctactctaaa aagccagggt
     3121 attggagact ggagagattt actgcttgta gaggataagt taaaaaaggc agggggagaa
     3181 tttctgggag tcttgcctct ccatcttact gaaaatcgct ctccccacgg tattagccct
     3241 tatctacctt tggaccgatt gctctggaac cccatttatc ttcctcttga ggaggtagcg
     3301 tcttttctgg gatatgaaga aagtaagaaa tatttagatg agctttctcc tcttttttcc
     3361 tctttctccc cagaaaaaga gatagattat gacggagtgt ggtcgctaaa gcaaaaattc
     3421 ctgcgccaga ctttcggcct gttttgggaa agaagaaatg aagatccccg ctttttagag
     3481 ttggaatctt tttttcagga agagagaata aaatgggctt ctttctttta tgcctttgct
     3541 gaagttcatg gtttaaattg gagaaattgg ccctctccct accgatataa agaaaaaaag
     3601 gccgtagaag attatattca ggctcaccgg gaagaggtgc tatatcatgc ttatcttcaa
     3661 tggttgatgg aaaaatttct ctcttccggt tcttttttgg ggttcgattt accagtggga
     3721 gcctcggcgg gagggagtga gtgtttctta gaagaagatt gttttgcttt tcaagcttcc
     3781 ctgggagctc cgcctgatga ttttgctccg gcagggcaaa actggggatt tcctcctccc
     3841 tttcccgaaa aagagagaaa aaacggctat cggcatttta ttgccctcat tcgtaaaaat
     3901 atgcaatttg ctcgctatct ccgggtagac catatcctgg ggtggcagcg tctttactgg
     3961 attcccgaag ggagcgaggc tcgggaagga gcgtacgtcg agaattttct tcctgattta
     4021 ctggggattt tagctttaga aagtatgcgg aatgaagtaa cagtaattgg agaagacctg
     4081 ggaactgttg accctctttt aaaagagagt ctgcacaagt ttcaggtgct ttctacccgg
     4141 gtgttttatt ttgaaaaaga cgcagagggt ttccctcttt ctccttcgca atacccccct
     4201 ctttctcttg ccacttttaa cacccatgac cttcctcctc tggtttcctt ctgggaaggt
     4261 gaggatattt tgctccggga aaaagtaggg atgtatggag aagaggaagt ccgggtgtat
     4321 ctgggagaaa gggaagaatt ttgcaaaaaa gtgcttcaaa agctttccca gtggggatta
     4381 tgggaagaag gagggagcct gtgggaggca ttgctgcgct ttttggctct cactccttcc
     4441 ttgatcaaag tggtggggtt aaacgatgtt ttaggagaga agagaatgat taatcttcca
     4501 ggaactgttg atgaatatcc caactggaga ctccgcctgc ccatccagga aactaatctc
     4561 gaggaggctc tgtgtcagat agggagaatt atgaaaggag gaggataaga tgcctgaact
     4621 tcctgaggtg gaagtaataa ggagagaatt gaaagaaaaa attgaggaaa gccgcattct
     4681 ctttgtccgg gtggaggatt ctcaatttta tcttccctct gatatgattt tagggaaaaa
     4741 aataaacaag ttagagcgga aagggaaaaa tttgttttta gttctggaag atgagagctc
     4801 tatatttttt catctgggaa tgacaggaag attgcttttc tcttccagag aagaaccttt
     4861 tccctatctt aaggttcttt taggtttatc ttctggcttt ctctccttaa ccgatcagcg
     4921 gaaattggga aaagtagcat tgctaaccag agaagaaaca gaagaacttt tacgccaggt
     4981 ggggatagac cctttgtctc ctgaatatac cgaggaaaat tttgaaaaag ttttgagcgg
     5041 aagaaaaatg agggttaaag actttctcct ttctcagcgc tacatttctg gtataggtaa
     5101 tatctatgct tcagaaattc tctttcggag ctctattcac cccgagaaaa gaacgaacga
     5161 tttgacacca gaggaaaaga aaaagctgtt tatcaccatt cctcaagttt tgaaagaagc
     5221 cattgctcac cagggaacta ctattcggaa ttacgtccgc tcagggggag agcaaggaga
     5281 gttccagaat tcattgttag tctatggaaa ggaaggagag cggtgtccct gctgtggaga
     5341 aaaaattgtg agaaaaaaaa ttgcctcccg cagcacttat ttttgtcctc gttgtcaaat
     5401 gggctaaaga ggggcatcat aggtaatgag ggggaaaccc tgataacccc gattatggaa
     5461 ggggacgatt ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctctggct
     5581 atattaccag ttataaaaga aatagttccc atggttaaaa attgcgcaaa accaactgtt
     5641 tttcctttaa acaaactcaa ccagaaattg gcaaagagca taaaactaaa tagggcaata
     5701 tcatgctttt ttaggaaata actcgcagat agggtagcga aaataaaccc tgtaaagtaa
     5761 cccggtgggg tcggcgagga tagcagtgcc tccttaccaa ccggtaaatc agggaaggcg
     5821 agagtgcctg acctgattta tatagccaag cttatgcctt tccaacgagt gcccgcgggg
     5881 gagtctgacc gagaaagaaa aacctctatc tgggctaaaa gacccacaat acaggtaaag
     5941 ccaaaactaa agtggtatgt tcaaccatgc tgccatctat aaaaatcata cctcattttc
     6001 tcatatattt ctcctctgtg gccattatcg tttttcacct tcctttttaa tttttccaat
     6061 aagtgaatac aatcaccggc aaatgtccgg gtgagagagc catcgactct ttttgttatc
     6121 gcttcacctg gtatttatgc ctttttccag atttacgaaa tttagagcat cggtttcagc
     6181 attcatttct gctaatatgc cacacacctt cttttctcca ttgagaaatc gttgggccat
     6241 tttggctctg cggccaaacc gaagaatttt ctaattttaa ccttcttctc ccaggccttg
     6301 agactcggca attattgttg ttccctcttt tatcctcggt gagatggttc ccttgtccca
     6361 tctgtggagc taacttcccg aaagtagtat atcttttgcc ctaaaccagg gagctcacaa
     6421 ggaaggagtg aatctggtga tatcctttag gcaaatattc taatatagaa ccatcttgtt
     6481 ttaaatcacg aatatgcttt caggttgcaa ctccggagac atccagttct ccagctatca
     6541 tttacccaga agcataatgt ccactttttc taagagtttt caatatcttt ccttttattt
     6601 ggttaactac attgtgtttt tttgtcaagg tctttcgggt ttgttcaaaa aatacctcat
     6661 agttttagag agtagggttt ctttgtatat tcagaaacat atctaccttt ccagattttg
     6721 agctattccc ttataatctc tttatcccgt cattttgtac cctaatattt tatgaaatgt
     6781 aaaattattt ataaagcaat cctttgtatt attgacatac tctcaattaa ttatataata
     6841 ggtaaggtaa taaaaattgc cagggggtgg aaatcgaggg ctaactaaaa ggtgcttttt
     6901 tgcgccataa tatcattgtc cctccgctaa cacccagatt agggaagcgc cccttcccta
     6961 aggggggagt gtcccctgat taatttgttt tcggataagc tgatccgaaa ggggagcacc
     7021 tgatgaaaag ggttattggg cttagattct ggtttctaag gatttaatgg gacgtggtca
     7081 gggaagcaaa tatttgtttt cctgaccatc gcttttgtta aaaatatcta tcctcactca
     7141 aattggtggg gatagatatt ttgttcttta gtacttttta gccaatggcc agtaaaaaat
     7201 tcgaagggag aagggaggga caacctgtgg agaaaatact ggaagtgggg atgcatctca
     7261 tccttgatgg ttattgtcag gactcttcga ttattggaaa cttaggcgaa gtgcataggt
     7321 tcctggatga gtttcccgag gaaatgggta tgaccaaagt tctaccgccc tacgttttcc
     7381 aatatcagag cagttctccc ctggatagtg ggatatcagg tatagtgata attgccgaaa
     7441 gccacatcag tattcacact tttccggttc ggaattactt aagtgtggac atattttctt
     7501 gtaaaccgtt tgatgttgaa gaggcaatac agaagctcaa tagctacttt aatctggtgg
     7561 aatacaacta tcaattgttt gatcggggga tagagtatcc tcctgacctt aactcgagtc
     7621 tccccttagt tatagaggag aggcttaaga acctggaaaa aatgggtcac acctgactca
     7681 ctacttattt ccccggtagg gtgagtgtat gtctcaataa gtcaaacact caccctattt
     7741 tttattctcc atcctggact ggcattccac acaatagaga gcatagggaa tcgttttcaa
     7801 ccgctgaata gatatttccc tcttacacaa ctcgcaaaga ccataatttc ccgactctat
     7861 ctttttcaaa gccttctcca ccaacctcag catctcaaga agggttttct gggaagacat
     7921 ctggagagcc agatctacag tggcacttcc caagtctgct tcatctcttg attcgctcaa
     7981 tgggttatag tctcgagtgg tatcacgttc tcgcttgaga actaatttta tctttttatt
     8041 caactccaaa agctgggcgc gaagtccttc tatttctcgc tcttccatag ctctcatctc
     8101 cgaggatata ataactcata ttttttcttt atacaacctt atcactcgct gtaccgctta
     8161 taggtcaatg gttacactaa tattactaaa attgctttat tagtagaata gaactgaact
     8221 atagagatgt tgcctttcct tcccaaaaaa tagccggtct cctttcatct ctgttttctt
     8281 gtcaagttca atccatcttg gtaaaatgat ttaagcaagc tttaatactc tttgtgtgat
     8341 gcggtggaat tactttaatg gtgagctttt caatctccgg ggcagaaagc ttatgaaatt
     8401 atggcagaaa taagtgtttg ctatcattta taaatcgttt gttaagaagg aggagttttc
     8461 tatgggacca aaagaaatat ctcagaagct agaccaattg ttgcggctgg atacgttccc
     8521 ggtaggggta aaattttaca ctcgggaaga agatttgcct aaaaagccgc ttccgttcaa
     8581 actcaacatc tgtcaactgg tctgtatggc gcgataccag aaaatgggcc atagcggcgt
     8641 tccagagaaa atggtatgtg ccttcggggc ggcatgcctg ggattaattc agacacctga
     8701 ggctatccta tccgggaaag ctgccgtggg tgcttattgc aaagatcagg aagcggcgaa
     8761 gaagttcatg gcaaacgtat acaggctggg agacgatggt aagaagtacg ctggagtatt
     8821 tatctccccg ctggaaacat tcaccgagga gcctgacgtg gtggtggtct acggcaatcc
     8881 tgcccagatg atgcgtttca ttcatgccaa cgcttatgac accggagaaa agaccacggc
     8941 tgatacggta gctgaagggg ctatgtgttc tgctatagga tttgccaaag ggactggtaa
     9001 acccacggtg ggtttcccct gtgctggtga cagaatcttc ggtggcacac agcaccatga
     9061 gcttgtctat gcagcacctt atgctctttt ccgtgataag ctcgtggaca acctggaatt
     9121 cacggcgaag ggaggtttct ccgtctatcc agtgccacca tacatggcct tcacaccaga
     9181 gatgccgccg gcttattcta taaagccgga ggatctataa gtctgtgtaa tgtgcgggaa
     9241 gctataaaac aaagacgctc cccactcagc ttgcaagcag gggagcgtct tctagcaaag
     9301 tcttcagaaa agggatgtgt ttataaattc tggctatact gactttcctg caagcgtagt
     9361 gtttatcacc cctatatagg aggcaatact cgcctcagag tgataaggat ttccagtttg
     9421 cataccaggc atcaccggta gagcctgctt tagctatcgc aacgccctga ggataacaat
     9481 gtttaatatg gttttttgtt ccttttggta tgtcgaaaga agatgggttg agaaatttta
     9541 gatgctttcc atatgtttgg gatttcacgg caatgaagag aattatatat aaaaacgtat
     9601 gtcatagtag agaaaaatgg aagagttaaa aaggaaagat tgatgacaaa tgacccatat
     9661 tgctgtaaca tatttaagaa taagaaaatt agatgttaaa ggggaagttc ttgagtaatt
     9721 tatgggtagg agataatctc tccgacgtaa aagttaaaaa tcgctctttg gtgttacgtt
     9781 tactgaagaa aaaagggcct ttatcccgaa ttgatttagc acgcatcact ggtcttactc
     9841 aacctacgat tacgaacatc gtcaacgacc ttttgtcggc caatctttta caggaagtgg
     9901 gtatttccga taccaaagcg gggagaaagc ccattcttct ttccgtgaat gaccaggctt
     9961 tctacatcat cgccatcaac tttagccggc gaggcttctc tattgccctt tgtgatctgg
    10021 gtcccaacat tcttttccgc tgtgattctt cttacagcct attagaaaat accgacatag
    10081 ctcttttaga acttcagaag gagttcatcc acattttgga gtatgctacc cagtctctcc
    10141 gtcttatttt aggaatcggt atcagtgtac ccggccctgt ggatgctgag tcctgtacta
    10201 ttttagctca tcctactttc ttgaaacatc gctccctgga tcttcgattt tatttgaggg
    10261 agtatgacct tcccattttt atgatgcacg atgctgatgc agcctgcctg catgagacct
    10321 ggagtggaga ggctcgagaa acccagaaca tggtctattt tatggtggga gagggagtgg
    10381 gggcgggaat tctcatagac ggaaaaattt atcgtggtca tcatcgtcag ggaggagaaa
    10441 tagggcatac cagcgtcaat ctttttggtc ctcgatgtgt gtgcgggaat tacggttgct
    10501 tagagagtta ttgtagtatt cccaatgtct taaaaaaagc gcaagaggtt gtctggtttg
    10561 gagaaagtag ttatttaagg gatatttctt ctcgtcaggg gggaatagaa tttcaccacc
    10621 ttttagaagg agcaaggatg ggagataaca cttccttgca gattctcaat caattgggtc
    10681 aatacgtggg agtgggaatg gtaaatttaa tcaacctcta tgacccggag atggcggtta
    10741 taggaggaga agtagctctg gcccaggagt tcattgctgg aacggtcaat caggtactgg
    10801 aagaaagact gctctaccga gaatatgtaa atccccaatt gcgctattct aaatggggaa
    10861 gagatgtcac acttttggga gcagcttcca ttgttctgga tcacttcatt gcgggagagc
    10921 tgggaaaatt ctagctcacc tttttctgat tattatccga ttgggtatgc aagcgataaa
    10981 gcctccgttg tcgggaattt tccccatctc gatgcaaatg tgatccgggc agggagagga
    11041 gctaacccat acctttcctc cttctatctt cactatcgtt tctcccagcg gcccttccag
    11101 ggttataact ttttctgtcc ggggagtaac tttcaattct atgatttggt cctgggtctc
    11161 cagtaaaata ctgtaaaaat ctcgagatga tgaatatttc ccccccaccc atattcctgc
    11221 tgctgcaaac agaggcaaaa taacccacca tatttttcgc ttcttcatta atgatttacc
    11281 attaattatt aatcacaaat tttcaaggag aaaaattttt gtagagctcc tctacccggg
    11341 aaataatagt ttttaccgga atacccagtt tttctgcata ccatactgct cctcctgctc
    11401 ctactccctc ttttacgaat cctcgctcat aatcagaaag tccggggaag gaggagtgag
    11461 aaaaatctaa aggagcagag taaacttcta cccctaattc ttgcgctatc tccttgaagt
    11521 gagcgctttt atcttctact acatagcggg tggtagctac aaaaagggga cgattataac
    11581 cttgatggcg aagcactgca ctcactgcta acatttgagt ccctccagct aaaaccacct
    11641 ggcattccgg tagagctcct tctaccaatc ctcctgccac cgcttgcatg gggtctccac
    11701 agctctccac tgcttttaat cctgctccct gcaaagaacc gtagctaata ccttgaaaag
    11761 agctaacctc ttgccacact ttctctttaa ggggaatggg gttatgggga aaggccgagg
    11821 agaccattcc attgtatcct aaagcccgaa ggacacaaag agctgaggtg gttcctccgg
    11881 gtaaagattc ggctatcacc aggcagtttt gagttttaga gagttcttct cctaacattt
    11941 ttcctttttc ccagagggaa aaagcttctt ctactgcttt tccccggcgg gggtctctcc
    12001 ctggagaagc ggcagtttcc acatagggaa cggtgggggg aagaagagtt cctgctcgca
    12061 ctataagcac tggaaaccca gcctccaggt aagccgctct acttatgaga gcgggggtgg
    12121 gatgtccctc cgggtcaacg ggaagagcat cgattatttg aggtttacct ttccagagca
    12181 cttcggcatc agctggagca gtatatgcca ccgcttctgg gttagaacca gctgctgaaa
    12241 gtccgggaat agttcctaat tcagttcctc caacgattaa aataaacacg tttacctcct
    12301 gtttaagaaa attattatat atatataatt taatcacttc tggggaaaaa ggggaagagc
    12361 ataaaaggat aaaagaagga gattaaagtg atatgtcggg gaattccccg acatatagcc
    12421 agttctagtt tttcagtcta cgttagagat ggtttactga ggaaggtttc ttctttcctc
    12481 aacgctggag cgtttttcct ccagcgtaat ttttaatctt ttgcgttttt atagtattcc
    12541 gcaaaaccta ttataattgc tattccttgc gggaaagaga aagcttctcc aaaggaaatt
    12601 cgggaagtct taaactccct gaa
//
LOCUS       DAOPSS010000038        12348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4341_length_12348_cov_7.354185, whole genome shotgun sequence.
ACCESSION   DAOPSS010000038 DAOPSS010000000
VERSION     DAOPSS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12348
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4341_length_12348_cov_7.354185"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKN31_03605"
     CDS             <1..337
                     /locus_tag="PKN31_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOA99049.1"
                     /translation="VLALWEAFGYTPKRVRVVGGGAKNKLWNQIRADVLNLPVVTTSC
                     EEATVLGASLFSQVGAGIVKNLEEARSQVKIETTVWEPSSQREIYEELFTRYVELPPL
                     LASFYRERC"
     gene            complement(340..768)
                     /locus_tag="PKN31_03610"
     CDS             complement(340..768)
                     /locus_tag="PKN31_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOA99050.1"
                     /translation="MTSGEYHDQGFNCCESTLQGLGDYLGVKSDLIPSIATGFGGGIG
                     HTGGVCGAITGAVMALGIKYGRRDPQDKEKRDWLYQRVENFLQDAEKELGSLNCRDLI
                     GLTLNTDEAMKIYREKNLRKKCHQIIEQVEKLAQTYLSLD"
     gene            complement(770..1957)
                     /locus_tag="PKN31_03615"
     CDS             complement(770..1957)
                     /locus_tag="PKN31_03615"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOA99051.1"
                     /translation="MAVNSGGSSIKYELFSIEEEKEQSLSRGSVKRLYRPDSFWEGEK
                     EGIKSRKEVPDLDHEKGLRLIIDILTQEGPLHSIQEIEAVGIKLINGGAEVTQTSFID
                     SGVVQALQDLSGVTSVHNPPSLLAIEMFQKILPQTPLVGVFETTFHLSIPLPYRTYGL
                     PWELSQKYGLKKLGFHGNSHRYIAERIASLTSRAQKVISCHLGSGCSVCAIKEGRSLD
                     ISSGFTPQSGTIMSTRPGDFDPQIITYLEEKANFSPQEINRILTRESGLLGISGLSGE
                     MWELEELSQKGEERAKLAVEVFVYQVKKYIGAFVALLNGLDSLVFTGGIGENDSFIRE
                     KVGNNLDYLGIKIDREKNQEIKGKEGKISSSTSPVEVWVIPTNEELMVARETAKIVKE
                     RRK"
     gene            complement(1944..2882)
                     /locus_tag="PKN31_03620"
     CDS             complement(1944..2882)
                     /locus_tag="PKN31_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA99052.1"
                     /translation="MKCTRCKKEALVKLKRHNAKFCGDCFEVFFSRQVESAIKRKNMF
                     SEEEKVLVGVSGGKDSMSLWHYLKKAGYEVVAIHFDLGIEEHSQKSRQIVEEFARQNS
                     FPLEIIEVKKEVGFSIPEIKKRIPYRPICSMCGLIKRYLLNFWAFREHFSVYATGHNL
                     DDEAATLLGNVLHWQIDYLSHQHPTLPSPHPKMVKKVKPFYTLTEEEIMIYIRLHNIP
                     FLEERCPLSRRAKSWDYKEALNFLEEKSPGTKHMFWLGFLETGQKYFRQEEPPLHLGE
                     CSICGMPTTQEVCSFCRLIQKVKEREEKYLDYNGGK"
     gene            3370..3510
                     /locus_tag="PKN31_03625"
     CDS             3370..3510
                     /locus_tag="PKN31_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOA99053.1"
                     /translation="MKITTDELCRRSGVSPSTVLRTLNGSSAISEETKRGVSPPGKRV
                     ES"
     gene            3516..3704
                     /locus_tag="PKN31_03630"
     CDS             3516..3704
                     /locus_tag="PKN31_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99054.1"
                     /translation="MVTHISSSFFPEVIYGVEDLAFRFAYNAEVSSLWGRREMPLVDS
                     RGMGKLVYQYCLPGRWFL"
     gene            3835..5097
                     /locus_tag="PKN31_03635"
     CDS             3835..5097
                     /locus_tag="PKN31_03635"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013708650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOA99055.1"
                     /translation="MPEQNHSYANMLKVLDACAEKLGVCWDDYVPLRYPERELTVSIP
                     VEMDDGHVKVFTGYRVQHSSVRGPCKGGLRYSSDVDLDEVRTLAALMTWKCAVVNIPY
                     GGAKGGVQCDPTKLSSQELMRITRRYTAMILPLIGPEKDIPAPDVNTNAQIMGWIMDT
                     YSAFKGFTVPGVVTGKPVEIGGSLGREDATGRGVLFTINQIALKKGIDLNKASFAVQG
                     FGKVGEAIARLLYQEGYRVVAISDISGGIYKEDGLDILDLSNYVKKNPSHLIEGYKAE
                     GISSLANQELLTLGVDILVPSARENQITEDNAGDIKAKIIVEAANGPTTPEADQILNE
                     KGITVVPDILSNAGGVVVSYLEWVQNIQCLMWDEKKVNSFLQEIMERSFQEVWEFHEQ
                     NKVPMREAAYMLALDRTIKARKIRGNFP"
     gene            5801..7057
                     /locus_tag="PKN31_03640"
     CDS             5801..7057
                     /locus_tag="PKN31_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOA99056.1"
                     /translation="MKKIFVVVTTAIFLVALLVVSAGAKIEISGLFMKQAGYQESEIR
                     DMTDEFLKQNPDIEVKLSFVSYEELHDKIVVSAASGVSTYDVILVDCIWPAEFAGAGW
                     LLDVTDRLPEEDRADIFPEVLSAVTYKGRLYGMPWLNDWEYLFYNKKMLEEVGASSPA
                     YWPEVIEISKKLKEANIVQYPIIDTWGQAESLVVAWLQYVVGMDGYPFDESGNPVMNQ
                     GAPLEALKFMVDNTKEGYFNPACIESFSEEVRRVFSSGQAAFALNWTYMYNLVNDPQE
                     SQIAGDAVLTLVPGFPEGRRSASCNGGMGLSIMQSSKNPDEAWQYVTYLASREVQKKY
                     SQSALPIWKSLYDDPELIAQQPEIIKVAKEQIPYVFDRPQLSWYSQFSVIMVEELQAA
                     LSGAKAPEQAMDEAVARCKEVMERYR"
     gene            7103..7999
                     /locus_tag="PKN31_03645"
     CDS             7103..7999
                     /locus_tag="PKN31_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOA99057.1"
                     /translation="MRPKNFRKKETYFAWLLILPAFVFIGIIIFYPMILSFCMSLFQI
                     ELTRGDIGTPFIGFRNYIEVLSSSYFWDSIGRTVYFTVVSIGLEMLFGFLIALLLNQE
                     FKGRGLLRSLLLLPWALPITVDAIMWKWIFNANYGALNAFLTQIGIIDSYRAWLTRPW
                     SAMHCVIVADVWKVTPLVALLLLAGLQTIPKELYEAAMVDGAGWQSYVFRILIPLLTP
                     TFLVVLVLRTLDAFRVFDIIYIMTQGGPANATKVISFLTYQEAFKFLNFGKGAALAYI
                     ITMFVALIALVYTRLMQRQVEY"
     gene            8011..8862
                     /locus_tag="PKN31_03650"
     CDS             8011..8862
                     /locus_tag="PKN31_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOA99058.1"
                     /translation="MGIKRQKRIRSVIIGIFSIILAAVILAPPLWLFISSIANLQELL
                     KIPLRWLPEKPSFERYREILFSQGPESEFRKTMWNSFFIALFVTIICVSLGSLAAYAF
                     SRLRFPGKEKILFVLLFSYMLPPIAVIIPIYRIFSRYQMLDTKQALILIYSAILVPFV
                     IWLMRTYFDTIPKDLEDAAKIDGCSHLGTLFRVIFPLSAPGIVATSLFAFLMSWEEFF
                     IALILTSTPQAKTIPVAIAEFSGRHSIDFGMMATGGILAALPPILIAFVFQRYIVGGL
                     LSGAVKG"
     regulatory      9046..9142
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9236..10528
                     /gene="thiC"
                     /locus_tag="PKN31_03655"
     CDS             9236..10528
                     /gene="thiC"
                     /locus_tag="PKN31_03655"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOA99059.1"
                     /translation="MLKTQLDFARAGIVTEEVRCAAQNEAISPEELSRLIAAGVAVLP
                     KNVNHSFPHIKAIGHKLKTKVNANLGTSDECADLEFEKRKLSAAIKAQTDSIMDLSTG
                     GDLHLLRSFFLEESPVMVGAVPIYAVAAELARRGEPLEKMTEEMLFTSIEEQCRQGVD
                     YITVHCGITRESFRKLDSSGRIMPCVSRGGSIHLYWMRKTGKENPLYEHFDDLLEIAH
                     RYDVTLSLGDGLRPGTILDAIDGPQIEELMTLAELSKRARARGVQVMIEGPGHVPLSH
                     IQSHVQLQKRLCEGAPFYVLGPIPTDIAPGYDHITSAIGGALAASAGADFLCYVTPAE
                     HLSLPDENDVYLGVMAARVAAHVGDIAKDVPGAIERDKRISIARECLDWEGMMAEALD
                     PELVRCKFSLASDRQSCTMCGKLCAVKMVRGAYELQKE"
     gene            10534..11316
                     /locus_tag="PKN31_03660"
     CDS             10534..11316
                     /locus_tag="PKN31_03660"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOA99060.1"
                     /translation="MKLDEVTITKAIVERYFERLMDNLDIDVAIVGGGPSGLVAGYFL
                     AKAGHRVALYERKLSVGGGMWGGGMLFNEIVVQEDARHLLDEFGVRTVPYKEPGYYTA
                     SSVESVSTITSKAVKVGLSVFNCVSAEDVVVKDDKISGLVINWTATSLAGLHVDPLSV
                     RSRFVVDATGHDTEVVAMLEKKAPGKLSTPTGSIEGEKFMNPEEAERLTLENTREVFP
                     GLYVTGMACNATFGGPRMGPIFGGMLLSGEKVAHLILKELSR"
     gene            11391..11478
                     /locus_tag="PKN31_03665"
     tRNA            11391..11478
                     /locus_tag="PKN31_03665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11426..11428,aa:Leu,seq:gag)
     gene            11529..11602
                     /locus_tag="PKN31_03670"
     tRNA            11529..11602
                     /locus_tag="PKN31_03670"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11561..11563,aa:Gln,seq:ctg)
     gene            11931..12070
                     /locus_tag="PKN31_03675"
                     /pseudo
     CDS             11931..12070
                     /locus_tag="PKN31_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004436687.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         3334 a         2370 c         2868 g         3776 t
ORIGIN      
        1 cgttttagct ctctgggaag cttttggtta tactcctaaa agggtgcgag tagtgggagg
       61 aggagctaaa aacaagctgt ggaatcaaat cagggctgat gtattaaatc ttcctgtggt
      121 taccacttct tgtgaagaag ctactgttct gggagcgtct cttttctctc aggtgggagc
      181 gggaatagta aaaaacctgg aggaagccag aagtcaggta aaaatagaaa ccacagtgtg
      241 ggagccttct tctcagagag aaatatatga ggagcttttt acccggtatg tggaacttcc
      301 tcccctttta gcttcttttt atcgggaaag gtgttaagtt taatcgagag ataaataggt
      361 ctgagctaat ttttctacct gctcgattat ctggtgacat ttttttcgca ggttcttttc
      421 ccggtaaatt ttcattgcct catcggtatt tagagtgagg ccaatgaggt cccggcaatt
      481 aaggctacct aattcttttt ctgcgtcctg caggaaattt tctactctct ggtagagcca
      541 atctcttttt tctttatcct gggggtctct tcttccatat tttatcccta aagccatcac
      601 tgctccggtt attgctccac acactcctcc ggtgtgcccg attcctcctc caaagccagt
      661 ggcaatagag ggaattaaat cactctttac tcccaggtaa tcccccaaac cttgtagggt
      721 agattcgcaa cagttaaatc cctggtcatg atattcacca gaagtcattt tattttctcc
      781 tttctttgac tattttggcc gtctctcgag cgaccattaa ttcctcgtta gtgggtataa
      841 cccatacctc aactggagag gtagaagaac ttatttttcc ttcttttcct ttaatttctt
      901 gatttttctc ccggtcgatt tttattccta aataatcgag gttgttgcca actttctctc
      961 ggataaaact atcattttct cctattcctc cagtgaaaac caggctgtcc agaccattca
     1021 aaagagcaac gaaagctcca atatattttt taacttgata gacaaatact tctaccgcca
     1081 attttgccct ttcttctcct ttttgagata gttcttccaa ttcccacatt tctccactta
     1141 atcctgatat tcctaaaagc cctgattctc tggtaagaat acggttgatt tcctgaggag
     1201 aaaaatttgc tttttcttca agatatgtaa ttatttgcgg gtcaaaatct ccagggcgag
     1261 tagacatgat tgttccgctc tggggagtga agccgctcga gatatctaaa gaccttccct
     1321 ctttgatggc acaaacgcta catccactcc ctaagtggca gcttattact ttttgagctc
     1381 gggaagtaag agaagcgatt ctttctgcta tgtagcggtg agagttaccg tgaaatccta
     1441 atttttttaa gccatatttc tggcttaatt cccagggtag gccataggtg cggtagggta
     1501 agggtataga gagatgaaag gtggtttcaa agactcctac caggggagtt tgggggagaa
     1561 ttttttgaaa catttctata gctaaaagag aaggggggtt gtgaaccgaa gttactccgg
     1621 agaggtcctg aagagcctga acaacaccgg agtcgataaa agaagtttga gtaacttctg
     1681 ctcccccatt gataagcttt attcccactg cttcaatttc ctggatacta tgcaggggtc
     1741 cttcctgagt taaaatatcg atgattaagc gcaggccttt ttcgtggtcg aggtcaggga
     1801 cttcttttct gctctttatc ccttcttttt ctccttccca gaaggaatca ggacggtaca
     1861 atctctttac acttccccgg gatagggatt gttctttttc ttcttctata gaaaagagtt
     1921 catatttgat agatgaaccg ccactattta ccgccattat aatcaaggta tttttcctcc
     1981 ctttctttca ctttttgtat taaccgacag aaggaacaaa cctcttgagt ggtgggcata
     2041 ccacaaatag agcactctcc cagatgaaga ggtggttctt cttgccggaa gtatttttgt
     2101 ccggtttcta aaaatcccag ccagaacata tgcttggtgc ctggagactt ctcttctaag
     2161 aagttcagcg cttctttgta gtcccagctc ttggctctcc gggagagcgg acatctttct
     2221 tctaagaaag ggatattatg aaggcgaata taaatcatta tttcctcttc ggtgagagtg
     2281 taaaaaggtt taactttttt caccatttta ggatgagggc tgggaagagt ggggtgctgg
     2341 tgagataagt aatctatctg ccagtgcaac acattaccta aaagggtagc cgcttcatcg
     2401 tctaagttat gaccggtggc gtaaactgaa aaatgctctc taaaagccca gaagtttaaa
     2461 agataccttt taatgagacc acacatagag cagataggac gatagggtat cctctttttt
     2521 atctcgggaa tggaaaatcc tacctctttt ttaacttcta taatttccag aggaaaggaa
     2581 ttttgacgag caaattcttc tactatctgg cgagatttct gagagtgttc ttctattccc
     2641 aagtcaaagt gaatagccac tacctcatat cctgcttttt tgaggtagtg ccacagagac
     2701 atgctgtctt tccccccgga taccccgact aatacttttt cttcctcaga aaacatgttt
     2761 tttcttttta tggctgattc tacctggcga gagaagaaaa cctcaaaaca gtctccacaa
     2821 aatttggcat tatgtctttt tagcttgacc agagcttctt ttttacatcg agtacacttc
     2881 attttatctt cctttcattt tagatttaaa tgtagcacga ctcgtttgtc aagatatatt
     2941 tggctcctcg gaagatagag atacagtatc tcccgggttg tgcctggccg catcgagctt
     3001 ttggctataa aagctttcgc actccacctg agagcttatc ttaccattta tataccaggt
     3061 ttgagctatt tttagagtct gttttaatta ttttgatggt ataattccag cgtgtgtggt
     3121 tattccgctt tttttcctgt aaaaaatagc aattataata ggttttgcgg gatactacaa
     3181 tgaatgcagc agagtttgcc tgccaaaatt aatatgctaa gagaaaatac ctcaggtttg
     3241 aggtaaaaaa ctgctcttct tttctatacc acgaggtgct ttttttaaca cactttatta
     3301 tagagcctgt aatatcttgc aaatttcaaa aattgtgcta ttgtaagcac agaaaatgga
     3361 ggcagcataa tgaaaataac tacggatgaa ctatgtcgga gaagcggtgt ttctccttct
     3421 actgttttga ggacacttaa tggcagttct gcaataagcg aggaaacaaa aagaggagtt
     3481 agcccaccag gaaaaagagt agaatcataa ctcttatggt tacccatatc tccagttctt
     3541 ttttcccaga agttatttat ggagttgagg atttggcttt tcgttttgcc tataatgctg
     3601 aggtatcttc gctctggggc aggagagaaa tgcctctggt tgattcaaga ggtatgggaa
     3661 agctggttta tcaatattgt ttgcctggaa gatggtttct gtgatgtgga atatttattg
     3721 caaaagatac aacgtataca attagagaga acgagggagg taaattagtt gagcttagat
     3781 aaccgttgcg gtcacactga ggttcaaaat gaacaaaagt gttgtgattc ttctttgcca
     3841 gagcaaaatc actcttatgc gaatatgctc aaagtgttag atgcttgtgc agaaaagctg
     3901 ggagtttgct gggatgatta tgttcctcta cgttatccag agagagagct cactgtttct
     3961 ataccagtgg aaatggatga tggacatgtt aaagttttta ctggctaccg ggttcagcat
     4021 tctagcgtgc ggggtccgtg caaaggagga ttacgttatt cttcagatgt cgaccttgat
     4081 gaagtgagaa ccctggctgc tttgatgacc tggaaatgtg ctgtagttaa tatcccttat
     4141 ggtggagcca aggggggagt gcaatgtgac cccacaaaac tttcttctca ggaacttatg
     4201 agaatcaccc gccgctatac agcgatgatt cttcctttaa tcgggccaga aaaagatatt
     4261 cctgctcctg atgtaaacac caatgcccag attatgggat ggattatgga tacttatagc
     4321 gcgtttaaag gctttactgt tccgggagta gttacgggaa aacctgtaga aattggtgga
     4381 tcactgggga gagaagatgc aaccggtaga ggagtgttat ttactatcaa tcagattgct
     4441 ctcaagaagg gaattgattt aaacaaagca tcttttgctg tccagggatt cgggaaagta
     4501 ggagaagcga tagcccgatt gctctatcag gagggatatc gagttgtagc tataagtgat
     4561 atcagtggag gaatttataa agaagatggc cttgacatac tggatctatc aaactatgta
     4621 aagaaaaatc cctctcatct cattgaagga tataaagcag aaggtatttc atcccttgcc
     4681 aatcaggagt tactcacttt gggggtggat attttagtcc catcggctcg ggaaaaccag
     4741 attactgaag ataatgctgg agatataaag gcaaaaatta tcgtcgaagc agctaatgga
     4801 cccactactc ctgaagcaga tcaaattctc aacgaaaaag gcataaccgt tgtccctgat
     4861 attctatcta atgcaggagg agtggtagta tcttacttag aatgggttca aaatattcag
     4921 tgtttaatgt gggatgagaa gaaagttaat agctttctcc aggaaattat ggagagaagt
     4981 ttccaggaag tatgggagtt tcacgaacaa aacaaagtgc ccatgcggga ggctgcttat
     5041 atgctggctc ttgatcggac aattaaggcg agaaaaatac gagggaattt tccttgatgt
     5101 ttaaggcaag agagttaact gaaaacatta aaataaaagt tccccttcca aatagttgat
     5161 agagaagtta aataaaaaat agacaccgct tgcccttccg ggtaaggttt tatataagta
     5221 gagccaactc cgaggagtaa gcggtgtcct gtatacgacc tcatccatta tttgatttat
     5281 agcattggat gcccctcttc aatcattaaa aactcctttg cagggagcaa caaaactgga
     5341 gaagaaccag aaaactataa aggaaggcga ccgctaaaaa attagcttgc gtaatgtgat
     5401 gtgttatctt cctcctcgaa atatttttca ataccagcct cttttatttt attataatgg
     5461 accccgaaaa ttggacagag caataatgtg tatttagtct gcaaacggaa aggagaaaga
     5521 taagaaaaat gggtgacatc cgaaagaagc atgagcatat catcctttta acattgacca
     5581 gggttttcag tttacaatga gtttgctgag gaactgaaga aagcaggggt tcaaatcaga
     5641 gagttagaag agaggaggaa aatacgggca aacaggttag agagatccac cttaaaagtt
     5701 tttttgcctt gacatgggaa tctaacttaa catacaattt gtgtgtaggc tagtgttcca
     5761 tatttatcat gtttgaaaac tttttaaggg agggagtatt gtgaaaaaaa tatttgtggt
     5821 cgtgacaacg gcgatttttc tcgttgcctt gctggttgtt tcagccggtg ccaagattga
     5881 aattagtggg ctttttatga aacaggctgg atatcaggaa agcgagattc gtgatatgac
     5941 tgatgaattt cttaaacaaa acccggacat agaagtgaaa ctatcgtttg tatcttatga
     6001 ggaacttcat gataagattg tggtttctgc tgctagtgga gtaagcactt acgatgtaat
     6061 tctcgttgac tgcatctggc cagccgagtt tgctggggca ggttggcttc tcgacgtgac
     6121 tgaccgttta cctgaggaag accgagctga tatcttccct gaagttttgt cggcggtaac
     6181 ctataagggt aggttgtatg gcatgccctg gctcaacgac tgggagtatt tgttttataa
     6241 caagaagatg ttggaggagg taggagctag ttctcctgct tattggcctg aggtcataga
     6301 aatttccaaa aagctcaagg aagcgaatat agtccagtat cctattatcg atacctgggg
     6361 acaggcagaa tcactggtag ttgcatggct acaatatgtg gtgggtatgg atgggtatcc
     6421 atttgatgaa tctggtaatc cggttatgaa tcaaggtgct ccactcgaag cgctgaaatt
     6481 tatggttgat aatacaaaag agggttattt caatcctgct tgtatagagt ctttctcgga
     6541 agaggtaaga agggtatttt cctctggtca ggctgctttt gctctaaact ggacctatat
     6601 gtataacctg gtgaacgatc ctcaagaatc acagattgct ggagatgctg tgttgactct
     6661 tgtccctgga tttccagaag gaagaagaag tgcatcctgc aatggtggta tgggcttgag
     6721 cattatgcag agcagtaaaa atccggacga agcctggcaa tatgtgactt atcttgctag
     6781 cagagaggtt cagaaaaaat acagccagag tgctctacct atatggaaat cgctttacga
     6841 cgatccagaa cttatagcgc aacagcccga aattatcaaa gttgccaaag agcagattcc
     6901 ctacgtgttt gatcgtcctc aactttcctg gtattcacaa ttttctgtga ttatggttga
     6961 ggaattgcag gcagcactca gcggggctaa agctccagag caagctatgg atgaagcagt
     7021 agccagatgt aaagaagtta tggaaagata tcgctaatat ttgggcgggg tggaaaaacc
     7081 ccgccctctg tagaagacct tcatgagacc taaaaacttt agaaaaaaag aaacatattt
     7141 tgcctggctt cttatccttc ctgcttttgt ttttataggt attataatct tctatcccat
     7201 gattctctct ttttgtatga gcctttttca gattgaactt actcggggag atataggaac
     7261 tccttttata ggttttagaa actatatcga agttttgagt agctcttatt tttgggattc
     7321 tataggaagg acagtctatt ttactgtagt gtccattggt ctcgagatgc tttttggttt
     7381 tctaattgcc ttgcttttaa accaggaatt caaaggtaga ggtttgttga ggagtctttt
     7441 attattgccc tgggctttac ctattacagt agatgccatt atgtggaagt ggatttttaa
     7501 tgctaattat ggggcgctta atgctttttt aacccagatt ggtatcatag attcttatcg
     7561 tgcctggcta actcgtccct ggagcgctat gcactgtgta attgtagctg atgtgtggaa
     7621 ggtaacccct ttggttgctt tattgcttct tgcaggatta cagaccatac ccaaggagct
     7681 ttatgaggca gcgatggtgg acggtgcagg ctggcagtca tacgtttttc gtatacttat
     7741 ccccttgctt actcccacct tccttgtggt tctggtgctt agaactctgg atgctttcag
     7801 ggtctttgat attatctaca tcatgactca aggcgggcca gcgaatgcta ccaaggtaat
     7861 ttctttcctg acctatcaag aggcgttcaa gttcttgaat tttggcaagg gagctgctct
     7921 ggcttatatt atcaccatgt ttgttgcctt gattgctctc gtgtacacta ggctgatgca
     7981 aagacaagtg gaatattgag gtgaatcagg atgggcatta agcgtcaaaa gaggattcgt
     8041 tcggttatta ttggtatatt ttcaataatt ctggctgctg ttattctggc accacctctc
     8101 tggttattca tatctagtat tgctaattta caggaactcc tgaaaatacc tctgcgttgg
     8161 cttcctgaga agcccagctt tgagcgatat agagaaatac ttttttctca aggaccagaa
     8221 agtgagttca gaaaaaccat gtggaatagt ttttttatag cactttttgt taccattatc
     8281 tgtgtctctt tggggtcatt ggctgcctat gctttttccc gccttcgatt tcctggtaaa
     8341 gaaaaaatcc ttttcgtcct acttttttct tatatgctac cgcctattgc ggtgataatt
     8401 cctatttatc ggatttttag tagataccag atgctcgata caaaacaggc tcttattctg
     8461 atttattcgg ctattttagt tccatttgta atctggttaa tgcgcactta ttttgatact
     8521 attcccaaag acttagaaga cgctgcaaaa atcgatggat gttctcactt aggtacgcta
     8581 tttcgagtta tttttcctct ttctgctcct ggaattgttg ccacttcact ttttgccttc
     8641 ctgatgtcat gggaagagtt tttcattgct cttattctta cctctactcc tcaggctaag
     8701 accataccgg tcgctattgc cgagttttcg ggaagacatt ctatagattt cggaatgatg
     8761 gctactggag gtattctggc agctttacca ccgattttaa tagcttttgt ttttcagaga
     8821 tatatagttg gaggattgtt gtctggagca gtaaagggtt agttgttatt tttttcaaaa
     8881 ccagcaaaga aaaattcaaa atatcagttt ccctgctaaa agctcttgtt gtgatgtttc
     8941 cagatgtttt ttttaggttt acgaaagttt actccttctc atctggtcgg tgtagtcgat
     9001 tgacgcaaaa aagaaacagt ggtagtatta ctgaaggcaa tttgtatgtc gccatggggg
     9061 cgctcttgaa gctcgggctg agaatgtacc ccttgaacct gatccaggta atactggcgg
     9121 aggaaatggc gggaagaagt taagagtata gcttcggttt aatttttgag ctgctttttt
     9181 tttatcttct ttttagtcgc tttttcctct tctaaaatta tacaggagga atagcatgtt
     9241 gaaaacccag cttgattttg ccagggcagg cattgtaact gaagaggtac gttgtgccgc
     9301 ccagaatgaa gcgataagtc ctgaagagct ctcacgtctc atagctgcag gggttgcggt
     9361 tctacccaaa aacgtgaatc attctttccc tcacataaag gcaattggcc ataagctaaa
     9421 gacaaaagtg aatgccaatc tggggacgag tgatgagtgt gcagaccttg aatttgaaaa
     9481 gcgcaagctt tctgctgcta taaaagcgca aactgactcc attatggacc tctcgaccgg
     9541 gggagacctt catcttttga ggtctttttt cttagaggaa tcaccggtca tggtgggagc
     9601 tgtacccata tacgctgtgg cggcagagct ggctcgaaga ggtgaaccgc tggaaaagat
     9661 gaccgaagaa atgcttttta cctcaataga agagcaatgc cgacagggtg tagactacat
     9721 caccgttcac tgtggcataa cgcgtgagtc ttttcgtaaa cttgactctt ccgggcgtat
     9781 tatgccctgc gtcagtagag gtggttcaat tcatctttac tggatgcgga aaactggtaa
     9841 agaaaaccct ctttacgagc attttgatga ccttttagag attgctcatc gctatgatgt
     9901 aaccttgagc ttaggagatg gcttgaggcc ggggacaatt ctcgatgcta tagatggccc
     9961 acagattgaa gagctcatga cccttgctga gctatcgaaa cgagcaaggg caaggggggt
    10021 ccaggttatg attgaaggtc cggggcatgt gccactttct catattcaat cgcatgtgca
    10081 gcttcaaaaa cgtttatgcg agggagcacc tttttatgtg ttgggaccta ttcccaccga
    10141 tattgctcct ggatatgacc acattacctc tgcgataggt ggagcgctgg cggcgtcagc
    10201 gggggcagat tttctgtgct atgtgacgcc agctgagcac ctcagtttgc cagatgaaaa
    10261 cgatgtgtat ctgggtgtta tggccgcgag agtggccgct cacgtgggag acatagctaa
    10321 agatgtgccc ggggcgatag aaagagataa aagaatctcc attgcccggg aatgcctgga
    10381 ttgggaagga atgatggcgg aggctttaga ccccgagctt gtgcgctgta aatttagttt
    10441 agcttctgac cggcaatcct gtactatgtg cggtaaactt tgcgctgtga aaatggttcg
    10501 tggagcatac gaattacaaa aggagtgata gaaatgaaat tagacgaggt caccataact
    10561 aaggcgatag tggagcgtta ttttgagagg ttgatggata acttggatat tgatgtggct
    10621 atagtgggtg gtgggccttc gggacttgtg gctggatatt tcttagctaa agcaggacat
    10681 cgggtggctc tttatgagag gaaactaagc gtgggcggag ggatgtgggg tggaggaatg
    10741 ttatttaatg aaatagtggt gcaagaagac gccaggcacc ttttggatga atttggagtg
    10801 cgaactgttc cttataagga acctggttat tatacagctt cttcggttga aagcgtgagc
    10861 actataacga gtaaagcagt gaaagtcggg ctttcggtgt ttaactgtgt gagtgcagaa
    10921 gatgtagtgg taaaagacga taagatttca gggctggtca tcaactggac agctacttcc
    10981 ctggccggtc tccatgtaga tccgctttcg gtgaggtctc gatttgtcgt agatgctaca
    11041 ggacatgata ctgaagttgt ggctatgtta gagaaaaaag ctccaggaaa gctctcaact
    11101 cccacaggaa gcatagaagg ggagaagttt atgaatcctg aggaagcaga aaggttgact
    11161 ctggagaaca ccagagaggt gtttccaggc ctttatgtga ctgggatggc ttgtaatgct
    11221 acttttggag gcccccggat ggggccaatt tttggaggta tgcttctctc aggtgagaag
    11281 gtcgctcact taatcttgaa ggagctttcc cgataaggcg aataatttat tttctgtatg
    11341 tagtctgtaa cattgacgag agggctttac cttgttataa ttctctgtga gccgggctgg
    11401 tggaaccagg aagacacgct atcttgaggg ggtagtgggt ttatgcccgt gcgggttcaa
    11461 gtcccgcgcc cggcaccacc tgaaaatcac aaaggtggta aggcggtata ttggaaaaaa
    11521 ggaatggttg agaggtcgtc taagggtagg actcgtgact ctggatcacg cagtggtggt
    11581 tcgaatccac ccctctcagc caacttttcc cgacgcaatc tccctattga gtgacctacc
    11641 tgaccgggcg taacctcggt taagacaaac ctctcgattt tcatcttggg ctcttctcca
    11701 attcagttgc tctcggtaaa agagccttta gtgactggag agaagtgttc ggtaatcaca
    11761 gttaaataaa accatttgcc ctccaaaagc aaatacttac ttcttttgat tgcctcttat
    11821 ccttttctcg cgggatgcct atatatcttt ttaggagaag aacccttctt tgcaaattta
    11881 tagagtaata gctgtctact caagcttttt gtcactgtga gaaacctcct ttggaattca
    11941 gcaagtggaa tggaatattt tatgatgaga taagacagcc gccatcattg accgtttagt
    12001 tcatcacagt catttactgg tcttttccgg accaagttat cctttgcctc attctactat
    12061 gaactgttag ttctggaatt aaaggtgttg caaaaattaa cttccggaat actgcaagtt
    12121 catattacca aatacacctc tgcgaggagt ctcctttttt gtcgtcacga ggagcaggtg
    12181 gcctacggcg taagattaaa agcgaaataa ataaaaaaac taaaaatcaa taatgctggg
    12241 aaaatacatc ccagctctga taaaatctct cttttgaatc tttggcaaat actaaaagca
    12301 aattagatag agtaaaaaac ttgggattgc cacgtaagcg aaaccacg
//
LOCUS       DAOPSS010000039        12253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4373_length_12253_cov_9.996475, whole genome shotgun sequence.
ACCESSION   DAOPSS010000039 DAOPSS010000000
VERSION     DAOPSS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4373_length_12253_cov_9.996475"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKN31_03680"
     CDS             complement(<1..341)
                     /locus_tag="PKN31_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA99061.1"
                     /translation="MGEDKVLVEIEREIVGGDFQSAGDVSSELKKVLQQVGFPPSFNR
                     RIVIAAYEAEMNVVIHAYRGVFTTQIYEDQVHIEVDDQGPGIPDLELAFQEGYSTAPP
                     HIREMGFGAGYG"
     gene            complement(342..692)
                     /locus_tag="PKN31_03685"
     CDS             complement(342..692)
                     /locus_tag="PKN31_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOA99062.1"
                     /translation="MTLREIKEILQAEVLAGEEFLDREPSSACGSDLLSDVLAFTKEN
                     SVLLTGLTNSQVIRTAEMSDIIGVIFVRGKKPDNYTIELATIKKIPLLTTRLPMYESC
                     GRLYLRGLKGCSEV"
     gene            complement(704..1300)
                     /locus_tag="PKN31_03690"
     CDS             complement(704..1300)
                     /locus_tag="PKN31_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOA99063.1"
                     /translation="MSNPFAFLKRRDPVETIIILLGGLLVVVIAFFIARGDLDYYTEY
                     LLTGGIFERKIEVKEVEVPFSSYFPEEEEFLPQETPQEMIPSPVPEGEEVSPPRVVAT
                     PFPEEKRVEPPQETKTPPPRVAEAPSGEFFVQCGAFSKPENAQKMVETLKGLGYQAVS
                     EPVSGLYRVRIYGFQSRDEAEKAVSKLKSQGIESFLGK"
     gene            complement(1300..3933)
                     /locus_tag="PKN31_03695"
     CDS             complement(1300..3933)
                     /locus_tag="PKN31_03695"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOA99064.1"
                     /translation="MNERTIISPVFEPSSVEDKWYEFWEEKGYFTPPPSPAPPFSIVI
                     PPPNVTGHLHMGHALNLTLQDILIRYRRMQGYRALWVPGTDHAGIATQNVVEKQLSRE
                     GLSRHDLGREKFLERVWEWKERYHERIVKQLKKMGASCDWTRERFTLDEGLSRAVREV
                     FVRLFEEGLIYKDEYIVNWCPRCETALADIEVEYTEVPAHLYYVRYYLKDEDNYLVVA
                     TTRPETMLGDVALAVNPHDERYKDLVGKIAILPLTGRELPIIADEYVDIDFGTGVLKV
                     TPAHDPHDFELGRKHGLPLVKVIDEQGFMSEEALNFSGLSREEARERVVEKLKEEEYL
                     EKIEDYSHSVGHCYRCSTVVEPLVSKQWFVRVKDLAQPAIEVVKQKKIEFVPERWEKV
                     YFEWMENIKDWCISRQLWWGHRIPVFYCQDCGYVFAHRDTPESCPRCGGEVKQDEDVL
                     DTWFSSALWPFSVFGWPEDTEDLRTFYPTSVLITGFDIIFFWVARMIMMGLKFMGDVP
                     FRKVYITPLLRDAQGKKMSKSSGNAIDPLEIIDQYGADALRFALGWLTVQGRDINLSR
                     ERIEASRNFMNKLWNVSRFVLMNEEGFSAFEGLVDLDIKDRWIISRFHRLLGEVEEAL
                     DSLDFGRYAQLLYDFVWGEYCDWYIEWSKIDLYQGGERKKRKAQNVLIYLLSGILKLL
                     HPVVPFITEEIWQNLPVKERESIMIAPWPYKEEKWLDEEAEKTIYFLQDVIREIRYLR
                     AELGIPPKEECQVILNLSDEESYRYMKGNFSYVESLAKCSILEAGYNLKKPESVATGR
                     VQGADIYLLVGGVIDLNKEVERLNKKLSRLQKEEERLRERLNNFSFLHRAPEEVVEKE
                     KKRFADLIKEKEKLETLLEGI"
     gene            complement(3946..5199)
                     /gene="clpX"
                     /locus_tag="PKN31_03700"
     CDS             complement(3946..5199)
                     /gene="clpX"
                     /locus_tag="PKN31_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOA99065.1"
                     /translation="MPKYEEEKLKLRCSFCGKNQSEVKKLIVGPGVYICDECVELCNG
                     IIKEDDKHRKSDLALEKLPSPKEIKAFLDQYVIGQERAKKVLSVAVYNHYKRITYSSR
                     EDIEIEKSNILLIGPTGSGKTLLARTLSLLLNVPFAIADATTLTEAGYVGEDVENILL
                     RLIQNANFDIKKAERGIIYIDEIDKIARKGEGPSITRDVSGEGVQQALLKILEGTMAN
                     VPPQGGRKHPHQEFIQINTENILFICGGAFVGLDKIVESRLKDHKIGFGAREASSTTD
                     SFNPLSEVQPEDLIRYGVIPEFVGRIPVVCALDPLGEEDLVRILIEPRNSLVKQYQSL
                     LKMEGAELIFTEGALRLIAQEAMAKGTGARGLRAIMEKLMVELMFELPSRKDIEKVVI
                     NEEAVKSGTLLPFLEGESAKEEQSA"
     gene            complement(5206..5832)
                     /gene="clpP"
                     /locus_tag="PKN31_03705"
     CDS             complement(5206..5832)
                     /gene="clpP"
                     /locus_tag="PKN31_03705"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOA99066.1"
                     /translation="MKDFLLMNSYLVPMVVEQTPHGERAYDIYSRLLQDRIVFLGTEI
                     DDTIANLVIAQLLFLEAANKEKDINLYINSPGGLVSSTLAIYDTIQYINPDVATICIG
                     QAASGAAVILAAGAPGKRMALPNSRVMIHQPLGGAQGQVSDIEIHTREMIKIREKLNE
                     ILAHHTGKPVEKIRNDTERDYFMSPEEARKYGLIDKVLYPEEKNKKEK"
     gene            complement(5829..7133)
                     /gene="tig"
                     /locus_tag="PKN31_03710"
     CDS             complement(5829..7133)
                     /gene="tig"
                     /locus_tag="PKN31_03710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOA99067.1"
                     /translation="MVEVKKEEKEKHVVEMEITVEKERVNEALDKVYRELSYRMTVPG
                     FRKGKAPRGILRSFAGEDFILETMTQDLIPQVVQEVLTQESIRVIGEPDLEIVHVEED
                     EPLVLRLQVIEEPEVKLADPGELEVRKYRLEIRESDVEKEVEKLIDSRSTWKDKGEGE
                     PAREGDLVMLKIEDEDYAVVAESSLDKEKSIAREVLGMRVGEKGKITQGEEEETEIEF
                     SISGIMGKERPEVNEEFLSGLEEGLNSIEDLKNRVREKLEALAQDIVEERLEKEAVVA
                     LCRESEVYIPEALLDIDVRQRIDILKEDLEKDGLTLDRYLELMDMDFATLEKNLRDLG
                     RWELRKFFVLEKYAEENEILVTEEDLMAEFEEIAKKTGKTKEEIKEILELSDRIEGVK
                     SNIRDRKTIKDIAQKVKVKELEEPINLDQWKALGDPEEEMIR"
     gene            complement(7327..8058)
                     /locus_tag="PKN31_03715"
     CDS             complement(7327..8058)
                     /locus_tag="PKN31_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOA99068.1"
                     /translation="MKVAILADIHGNFDALERVSEEIEKLEDVMVLGDIIGYGAEPED
                     CVRWVVEKKAKVLLGNHEAACIGELPLSWFNPLALRALLWTQKRLSSFSIRFIRSLPP
                     VISDFLQALWVHGSPRQPTEEYIDHPFIAREIFESFSFSLCFFGHTHQAEAYILEESG
                     RVERISFREGGKLEILPDNRYLINCGSVGQPRDGNPQASFAIFDTEKRIVEIKRVDYD
                     ISSASYKILHSGLPEYLAYRLEGGR"
     gene            complement(8060..8680)
                     /locus_tag="PKN31_03720"
     CDS             complement(8060..8680)
                     /locus_tag="PKN31_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOA99069.1"
                     /translation="MRLNVNPNRMELMKLKSRLSMAERGHKLLKDKLDALMQDFIRTV
                     RENRYLRQEIEEEIVKAFEAQSKASLLLSEEDKKLITVFPGERLGLEVQVRHVTGVKV
                     PHFEVKVEGDPYTYSLINTPAEIDEAFSSFFQVFPLMVKLAEAEKTIELLAVEIEKTR
                     RRVNALEYVLIPNLQETIKYITLKLDELARSSVVSIMRIKGKGLTG"
     gene            complement(8692..10083)
                     /locus_tag="PKN31_03725"
     CDS             complement(8692..10083)
                     /locus_tag="PKN31_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOA99070.1"
                     /translation="MYKEFTTISEVAGSLLTVEEVEDARYMEIVEIELQDGSRRRGQV
                     LMTSRGRALVQVFEGTEGIGVGQSKVRFLGRVMELSVSPDILGRVFNGSGVPIDGGPG
                     IVPDARLDVNGNPINPVTRDYPIDFIQTGISAIDGLNTLVRGQKLPIFSASGLPHARL
                     AAQIARQAKVLGEEEKFAVVFGALGITFEEANYFISELRNTGAIERSVIFVNLADDPV
                     VERIITPRMALTAAEFLAFEMDMQVLVILTDMTNYCEALREVSAARREVPGRRGYPGY
                     LYTDLATMYERAGRIRGKKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILNRQLQ
                     HKGIYPPIDVLPSLSRLRDKGIGEGKTREDHPDVANQLFAAYARGREAEELATILGEA
                     SLTDLDRLYMRFAEEFEEKFVQQGEYEDRSIEDTLSRGWDLLRMLPREELKRIQDIHL
                     EHFYNGQSSSSEE"
     gene            complement(10076..11863)
                     /locus_tag="PKN31_03730"
     CDS             complement(10076..11863)
                     /locus_tag="PKN31_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOA99071.1"
                     /translation="MMLEGSAKGTILSVNGPVVVAIGLPGAKMYDLVKVGEKKLVGEI
                     IGLDQDKATIQVYEETAGIRPGEPVESTYEPLSVELGPGLIGNIFDGTQRPLEVIREI
                     SGDFIARGVEVPPLDRSKKWDFHPLVKVGEEVEPGDILGEVPETTAITHRVMVPPGVR
                     GKVKEIKEGSFTVEEVIAIVDQEGEEKPITLIQRWPVRTPRPYKRKINLDEPLITGQR
                     VIDTFFPIAKGGVACIPGPFGSGKTVVQHQLAKWSDADVIVFIGCGERGNEMTDVLME
                     FPELVDPYTGEPLMKRTILIANTSNMPVAAREASVYTGITMAEYFRDMGYKVALMADS
                     TSRWAEAMREISGRLEEMPGDEGFPAYLGSRIASFYERAGKVVALGKEDRTGSLTVVG
                     SVSPPGGDLSEPVTQNTLRVVQVFWGLEDRLAYQRHFPAINWLISYSLYLPHLENFWR
                     ENVAPDFSANRNLAMQILQQEAELEEIVRLVGVESLSFRERFVLEIARSIREDFLQQS
                     AFQEVDTFSSLRKQYLMLKLIITYYQEGLKALEKGLSLRDILSIPSREKIGRAKYIPE
                     EKIETLEKLIEEVQEEIANKLRGEVESNV"
     gene            complement(11875..12192)
                     /locus_tag="PKN31_03735"
     CDS             complement(11875..12192)
                     /locus_tag="PKN31_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOA99072.1"
                     /translation="MSDSRLAFVGGEENSLCFRGMGFDVYVAYSAEEAKGFLPLLRRS
                     NYALIFTEWKFYSLIKDHFEDQRSEALPVILGIPTRKEEIGKGSEFIRELVRIAAGSD
                     ITL"
BASE COUNT         3401 a         3175 c         2058 g         3619 t
ORIGIN      
        1 ccgtatcccg ctccaaaacc catttctctt atatgaggag gagcggtaga ataaccctct
       61 tgaaaggcca gctccaggtc tggtatgcct ggaccctggt catccacttc aatgtgcacc
      121 tgatcttcat aaatttgagt ggtaaaaacc cctcgataag catgaatcac cacattcatc
      181 tctgcttcat aagcggcaat cactatccga cgattaaaag aaggagggaa gcctacctgc
      241 tgaagaactt ttttcaattc actggacaca tcaccggcac tctgaaaatc tccccccaca
      301 atttcccgct ctatttccac taaaacttta tcttctccca ttcacacttc actacaaccc
      361 ttcaatcccc gtaaataaag cctcccacag gattcataca taggcaaacg agtagttaaa
      421 aggggaatct tcttgatggt ggctaactcg atggtgtaat tatcgggctt ctttcctcgc
      481 acaaaaataa cccctattat gtcactcatt tccgcagtac gaattacttg agaattggtt
      541 aagccagtaa ggagtacaga attttcttta gtaaaagcca gaacatcact taaaaggtcg
      601 cttccgcagg cagaggatgg ctctcgatcc aggaattctt cccctgctag cacctcagct
      661 tgtaatatct cttttatttc tcgcaaagtc atataatttt tccctacttt cccaaaaaag
      721 attctattcc ctggctcttt aacttgctaa ccgctttctc agcctcgtcc ctgctttgaa
      781 aaccatatat tctaactcta tacaatcctg aaacaggctc agaaacagct tgatagccaa
      841 gccctttcaa ggtctccacc attttttgag cattctctgg tttagaaaat gctccacact
      901 gaacaaaaaa ttctcctgaa ggagcttcgg ctacccgggg aggaggagtt ttagtttctt
      961 gaggaggctc aacccttttt tcttccggga aaggggttgc aactacccgg ggaggagaaa
     1021 cttcttctcc ctctggtact ggagaaggaa tcatctcctg gggtgtttct tgaggtaaaa
     1081 actcttcctc ttcaggaaaa taagaagaaa aaggaacttc tacttctttt acctctattt
     1141 ttctctcaaa aataccacca gtaaggagat attcggtgta ataatccaga tctcctcgag
     1201 ctataaaaaa agcaataact actaccaata agcctccgag aagaattata attgtttcta
     1261 ccggatcccg tcttttcaaa aaagcaaagg ggttactcat cagataccct caagcaaggt
     1321 ctctaatttt tccttctcct taattaaatc agcaaagcgc ttcttttcct tttctactac
     1381 ttcctccgga gctcgatgca gaaaggagaa gttattaaga cgttctctca gtctttcctc
     1441 ttctttttga agacgggaaa gttttttatt caaacgctcc acctccttat tgaggtcaat
     1501 cacacctccc accagaagat aaatatccgc tccctgcact cttcctgtgg ccactgattc
     1561 tggctttttc aaattataac cagcttccag tatcgaacat ttagccaggc tttccacata
     1621 agagaaatta cctttcatat atcggtagct ttcctcatca gataaattga gtattacctg
     1681 gcattcttct ttgggaggga tacccagttc cgcccgtaaa tagcggattt cccttataac
     1741 atcctgtaaa aagtaaatag ttttctctgc ctcttcatct aaccatttct cttctttata
     1801 aggccagggg gcaatcatga tgctctctct ttctttcaca ggaaggttct gccagatttc
     1861 ttcggtaata aaaggaacca cagggtgcaa aagctttaaa atccctgaaa gtaaataaat
     1921 gaggacgttt tgagcttttc ttttttttct ctctcccccc tggtaaaggt ctatcttgct
     1981 ccactcaata taccaatcac aatattctcc ccatacaaaa tcgtaaagaa gttgagcata
     2041 tcgaccaaaa tctaaactat ccagcgcctc ctctacttca cctaataaac gatgaaaacg
     2101 agaaataatc cagcggtctt taatatcgag gtccacaagt ccttcaaaag ctgaaaaacc
     2161 ttcttcattc atcaatacaa aacgagaaac attccacaat ttgttcatga aattacgtga
     2221 tgcttctatt ctctcccgag agagattaat gtctctacct tgcaccgtaa gccaacccag
     2281 agcaaagcgc aaagcatccg ctccgtactg gtctattatt tctaaaggat caatagcatt
     2341 gcccgaagac ttgctcattt tctttccctg agcatcccgc aataagggag taatataaac
     2401 tttccggaag ggaacatctc ccataaactt caggcccatc ataatcatac gtgctaccca
     2461 gaaaaaaatg atatcaaaac cggtaattaa aactgatgtg ggataaaaag tcctcaggtc
     2521 ttcggtatct tctggccacc cgaacaccga aaaaggccag agagcagagc tgaaccaggt
     2581 atccaggacg tcttcatcct gctttacttc ccccccacaa cggggacagc tctccggggt
     2641 atctcgatga gcgaaaacat aaccacaatc ctgacaataa aaaacaggaa tgcgatgtcc
     2701 ccaccacaac tggcgtgaaa tgcaccagtc tttgatattc tccatccatt caaaatagac
     2761 tttttcccag cgctcaggga caaactctat ctttttttgt ttaaccacct ctatggctgg
     2821 ctgagccagg tccttcactc gaacaaacca ctgtttagaa accagaggtt ctaccacagt
     2881 agaacagcga taacaatgtc ccactgagtg agaataatct tctatttttt ccaagtattc
     2941 ttcttctttt aatttttcca ccactctttc ccgggcttct tcccgagaaa gaccagaaaa
     3001 gttgagagct tcttcgctca taaaaccctg ctcatcgata accttgacca gaggcagccc
     3061 atgttttctt cccaattcaa aatcgtgggg gtcatgagca ggggtaacct taagcacccc
     3121 ggttccaaaa tcaatatcca cgtattcatc ggcaataatg gggagttccc ttcctgtgag
     3181 cggcagaata gctattttcc ctaccaggtc tttatagcgc tcatcatgag ggtttactgc
     3241 taaagctaca tctcccagca tggtctccgg ccttgtagta gccactacca aataattatc
     3301 ttcatctttg agatagtatc tcacataata aaggtgagcg ggaacctccg tatactccac
     3361 ttcaatatca gccagtgctg tttcacagcg gggacaccag tttacaatgt attcgtcttt
     3421 ataaattaaa ccctcttcaa aaagtcggac aaaaacttcc cgcaccgccc gagataaacc
     3481 ttcatctaaa gtaaagcgct ccctcgtcca gtcgcaagaa gctcccatct tcttcaattg
     3541 ttttacaatt ctctcatgat aacgctcttt ccactcccag actctttcca gaaacttttc
     3601 tctgcctaaa tcatggcgac tcagcccttc tcgagacagt tgcttttcta ccacattttg
     3661 agtagcaatg cctgcatggt cagttcccgg tacccagaga gcccgataac cttgcattct
     3721 tcgataacgg attaatatat cctgaagggt gaggtttaag gcatgcccca tgtgaagatg
     3781 accggtaacg ttaggaggag gaataactat agaaaaagga ggagccgggg agggaggagg
     3841 agtaaaataa cctttctcct cccagaattc ataccattta tcttccaccg aagagggttc
     3901 aaaaactgga gaaataatag ttcgttcatt cattatgtat ttcccttacg ccgattgttc
     3961 ttctttagca ctttctccct ccaaaaaagg aagaagagta ccacttttga ccgcttcttc
     4021 attgatcact actttttcaa tatctttgcg agaaggcaac tcaaacatca actccaccat
     4081 taacttttcc attatagcgc gcaatcctcg tgctcctgtg cctttagcca tagcctcctg
     4141 agcaataagg cgcagtgcac cttcagtaaa aatgagttct gctccttcca tttttaacaa
     4201 gctctggtac tgtttaacca gactattgcg aggctcgata agaatacgta ccaaatcttc
     4261 ctcgcctaag gggtcaagag cacaaactac tggaatacgt cccacaaatt cgggaataac
     4321 accatacctt atcaaatctt ctggttgaac ttcacttaaa ggattaaatg aatccgtagt
     4381 agaagaagct tctcgcgccc caaaaccaat tttgtggtct tttaagcggc tttcaactat
     4441 tttatctaaa cctacaaaag cgcctccgca aatgaaaaga atgttttcag tattaatctg
     4501 gataaattcc tgatgaggat gcttcctccc tccctgggga ggaacattag ccatggtccc
     4561 ttccaggatt ttcaataaag cctgttgtac tccctcacct gatacatctc gggtgatgga
     4621 aggaccttct cctttgcgag caattttatc tatctcatct atatatataa ttcctctttc
     4681 cgcttttttg atatcaaagt tagcattctg aatcaaacga agcaagatgt tttccacatc
     4741 ctcccctaca tatccggcct cggttaaggt agtagcatcg gcaatggcga agggaacatt
     4801 aagcaacaaa gaaagggtgc gagccaaaag agttttacca cttcccgtcg gtccaataag
     4861 taaaatattg cttttttcta tctctatatc ttccctggag ctgtaggtaa ttcgtttgta
     4921 atgattgtag actgctacag aaagaacttt tttggctctc tcctgtccta tcacatactg
     4981 gtctagaaaa gcttttatct ccttgggaga aggtagcttt tccagagcca gatctgactt
     5041 tcggtgttta tcgtcttctt tgatgattcc gttacacaac tccacgcact cgtcacaaat
     5101 gtacactccc ggaccaacta taagcttctt aacctctgat tgattcttac cacagaagga
     5161 acagcgcaac ttcaattttt cctcctcata cttaggcatt attatttact tctccttttt
     5221 gtttttttct tccggataaa gaaccttatc tattaacccg tatttccgcg cttcttcggg
     5281 agacataaag tagtctcttt ctgtatcgtt tctaatcttt tctaccggtt ttccagtatg
     5341 atgggctaat atttcattca acttttctct aattttaatc atctcccgag tatgaatctc
     5401 aatatcagac acctgtccct gggctccacc taagggctga tgaatcatca cccgggaatt
     5461 aggtaaagcc atccttttac ccggagctcc agcagccaaa atcactgccg caccactggc
     5521 tgcctgtccg atgcaaatag tggctacatc cgggtttata tactggatgg tatcataaat
     5581 agctaaagta gaagaaacca aaccaccagg gctattgata taaagattta tgtctttttc
     5641 cttgttagct gcttctaaaa aaagtaactg agcaataacc agattagcta tcgtatcgtc
     5701 tatttccgtt cctaaaaaaa caatcctgtc ttgcaaaaga cgagagtaaa tgtcataagc
     5761 tctctccccg tggggtgttt gctccaccac catcggtact aagtaactat tcatcagaag
     5821 aaagtccttc atcttatcat ctcctcttcc gggtctccca atgctttcca ctgatctaaa
     5881 tttataggct cctccagctc tttgactttc accttctgag ctatatcttt aatagttttc
     5941 ctgtctctta tattgctttt tactccttct attctatcac ttaattccaa gatttctttt
     6001 atttcctctt tggttttacc tgtcttcttc gctatctctt caaactcggc catcaaatcc
     6061 tcttccgtaa ccaaaatctc attttcttca gcgtatttct ccagaacgaa gaatttccga
     6121 agttcccagc ggcctaaatc ccgtaaattt ttttccagag tggcaaaatc catatccatt
     6181 aactctaaat agcggtccag ggttaaaccg tccttctcca gatcttcctt cagaatgtct
     6241 atgcgctggc ggacatcaat gtccaacagg gcctcaggaa tataaacttc agattcccga
     6301 catagagcca ctaccgcttc tttctccaat ctctcctcca ctatatcttg agccagggct
     6361 tctaattttt ctctaactct gttttttaaa tcttctatgg aatttaaccc ttcttctaaa
     6421 ccagatagaa actcctcatt tacctctggc ctttcttttc ccattattcc acttatcgaa
     6481 aactctattt cggtttcttc ctcttctccc tgagtaattt tccccttttc tcctactctc
     6541 atacctaaaa cttctcgggc aatagatttc tctttatcta aagaagactc tgctaccaca
     6601 gcataatctt cgtcttctat ttttagcata accagatccc cttctcttgc tggttcacct
     6661 tcccctttat ctttccaggt actacgagag tctatcaatt tctccacttc tttttctaca
     6721 tctgattctc taatctccaa acgatacttt cttacttcta actccccggg atcggctaac
     6781 tttacttctg gttcctctat tacctgaaga cgcaaaacta aaggctcgtc ttcctctaca
     6841 tgcactatct ctaagtccgg ttcaccaatt actctaatac tctcctgagt caatacttct
     6901 tgaactactt gaggaattaa atcttgagtc attgtttcta aaataaagtc ttcgccggca
     6961 aagctccgca aaatacctcg gggggctttc ccttttctaa atcctggcac ggtcattctg
     7021 tagctcagct ctcggtaaac tttatccaat gcctcattta ctctctcttt ctccaccgta
     7081 atttccatct ctactacatg tttttctttt tcctcttttt taacttctac catttgtatc
     7141 cacctttctt aaactaaagt gttagatttg tataaggtat aataaaaaca ggagttatac
     7201 ctcaaatctt tctcattgag gtataaccca gttcattcga ttttatttta ctataatctc
     7261 tctggcagtc aagaaactca tctattccaa atagcgttgt ctcttaaatt agatttgtcc
     7321 ggaaacttat ctccctcctt ctaaccggta agctaaatat tcaggaagtc cactatgtaa
     7381 tattttataa gaggcagaag aaatgtcata gtctaccctt tttatctcca ctattctctt
     7441 ctccgtatca aaaatggcaa agctagcctg ggggttgcca tcccggggtt gccctacact
     7501 accacaattt attaaatatc tattatcagg aagtatctcc aattttcccc cttctcgaaa
     7561 agaaatcctc tctactctcc cactctcctc caggatataa gcttcagcct ggtgagtatg
     7621 cccaaaaaaa cagagagaga aagaaaaact ttcaaatatc tctcgagcta taaaaggatg
     7681 atcaatatac tcttctgtgg gctgacgagg actcccatga acccacagag cctgaagaaa
     7741 atcagaaatg acaggaggca gagaccgaat aaaccggata ctgaaggaac tcaaacgttt
     7801 ctgagtccag agcaaagctc gaagagcaag aggattaaac caggaaagag gtaactcacc
     7861 aatgcaagca gcttcatgat tgcccagaag tactttagct tttttctcta ctacccacct
     7921 cacacaatcc tctggttctg ctccatagcc aatgatgtct cccagaacca tgacatcttc
     7981 taacttctct atctcctccg ataccctctc taaggcatca aagttgccat gaatatcagc
     8041 gagaatggca actttcaact catcccgtca atccttttcc tttgattctc ataatgctta
     8101 ccaccgaaga acgagctaac tcatccagtt taagagtaat atattttatg gtctcctgta
     8161 aattgggaat caggacatac tctaaagcgt taacccgtcg gcgagttttt tctatctcca
     8221 ccgccaggag ctcgatagtt ttttctgctt ctgccagttt aaccatcaag gggaaaacct
     8281 ggaaaaaaga gctaaaagct tcatctattt ctgccggagt atttattaaa ctgtaggtat
     8341 aggggtctcc ttctaccttg acctcaaaat gaggaacttt tactccggta acatggcgaa
     8401 cctgaacctc aagccccaat cgctctcccg ggaaaacagt gattaacttt ttatcttcct
     8461 cagaaagcaa aagagaagct ttactctgag cttcaaaagc tttaacgatt tcttcttcga
     8521 tttcctgacg caggtaacga ttttctctca ccgtgcgaat aaaatcctgc atcagggcgt
     8581 ctaatttatc ttttaagagc ttatgacccc gttctgccat gctcaaacga cttttaagct
     8641 tcatgagctc catacgattg ggattaacat tcaatctcaa gggaaatcct cctattcttc
     8701 agaagaagaa ctctggccat tataaaaatg ttccaggtga atatcttgaa tacgcttcag
     8761 ttcctctcga ggtaacatgc gtaataaatc ccagccccgg gagagggtgt cttcaatgct
     8821 tcggtcttcg tactctccct gttgaacaaa cttttcttca aactcctcag caaacctcat
     8881 atacaagcgg tcaagatcag taagagaagc ctctcccaga atagtagcca gttcttccgc
     8941 ctctcttcct cgagcataag cagcaaacag ctgattagcc acatcaggat gatcttctcg
     9001 agttttacct tcaccaattc ctttatctct aagtcgagaa agagaaggca aaacatcaat
     9061 gggaggataa atgcctttat gctgcagctg ccgatttaaa attatttgcc cttcagtaat
     9121 ataaccagtg aggtcgggaa taggatgagt tttatcatct tcgggcatgg tgagaattgg
     9181 aatctgagta atagagcctt tcttgccccg gattctaccc gcccgctcat acatagtagc
     9241 caggtcagta tatagatatc caggataacc tcttcttccc ggaacttctc tcctagcagc
     9301 agaaacctct cgtaaagcct cacagtagtt agtcatatca gttaatatta ctaaaacctg
     9361 catatccatt tcaaaagcca gaaattctgc cgcggtaaga gccatgcgcg gggtaatgat
     9421 tcgctctacc accggatcat cagccagatt aacaaagata accgaccgct caatggctcc
     9481 ggtgttacgc agctcagata tgaaataatt agcttcttca aaagtaatac ccagagctcc
     9541 aaaaactacg gcaaattttt cctcttctcc taatacttta gcctggcgag caatttgggc
     9601 agccagacga gcatgaggta aaccagaagc agaaaaaatg ggaagcttct gtcctcttac
     9661 cagagtattc aaaccgtcaa tagctgaaat cccggtttga ataaagtcga taggatagtc
     9721 tcgagttaca ggatttatgg ggttaccgtt aacgtccaga cgagcatcag gaacaattcc
     9781 cggaccacca tcaataggaa ctcccgaacc attaaaaact ctccccaaaa tatcgggaga
     9841 aacagaaagc tccatcactc ttcctaaaaa cctgactttg ctttgcccga cacctatccc
     9901 ttctgttcct tcaaaaactt gcaccagcgc cctgcctctg ctggtcataa gcacctgacc
     9961 ccgccgtcgc gagccatcct gcaactctat ctccacaatc tccatatatc gggcatcttc
    10021 cacttcttct acagtgagca aagagccagc cacttctgaa atagtggtaa attctttata
    10081 cattgctttc aacctcgccc ctcagtttat tggctatttc ctcttgaact tcctctatga
    10141 gtttttccag agtctcaatt ttctcttcag gaatatattt cgctctccct attttctccc
    10201 gagaaggaat agaaagaata tctctcaaag ataaaccttt ctctaaagct tttaaacctt
    10261 cctgataata agtaataatg agtttcagca tcaagtattg tttacgcaaa gaactaaaag
    10321 tatctacctc ctggaaggca ctctgttgaa gaaaatcttc ccggatagat cgagctattt
    10381 ccagaacaaa tctttcccga aaagatagcg attctactcc cactaaacgc actatttcct
    10441 ctaactctgc ttcctgctgg agaatttgca tagccagatt tcgattggca gaaaaatcag
    10501 gagccacatt ttctctccag aaattctcca gatgaggaag ataaagagaa tagctaatca
    10561 gccagttaat agctggaaaa tgacgctgat aagccaatct atcttccagc ccccagaaca
    10621 cctgtactac tcgcagagtg ttttgagtca caggttctga taggtcacca ccgggaggtg
    10681 ataccgaacc aaccacggtc aaagaaccag ttctatcttc ttttcctaaa gcgaccactt
    10741 tcccagctcg ttcataaaaa ctggctatcc ggcttcctaa ataagcagga aacccttcat
    10801 ctccgggcat ttcttccaga cggccagaaa tctctcgcat agcctctgcc cagcgagaag
    10861 tagaatcagc cataagagct actttgtagc ccatatcccg aaaatattca gccatggtaa
    10921 ttcccgtata aaccgaagct tcacgcgcag ccaccggcat attagaagta ttggcaatta
    10981 aaatagtcct tttcataaga ggttccccgg tgtaggggtc aaccaattcg gggaactcca
    11041 tcagcacgtc ggtcatctcg ttaccccgtt ccccgcaacc aatgaagact ataacatcag
    11101 catctgacca tttagccagc tggtgttgca ccactgtttt tccgcttcca aaaggaccgg
    11161 gaatgcaggc tactcctcct ttggctatag ggaaaaaggt atcgataact cgctgcccgg
    11221 tgattaaagg ctcatccagg tttatctttc ttttataggg acgaggagtc ctgactggcc
    11281 agcgctgtat taaagttatg ggtttttctt ctccctcctg gtcaactatg gcaatcactt
    11341 cttctacagt aaaagaacct tcttttattt ccttcacttt tccccgaacc ccgggaggaa
    11401 ccatgacccg gtgagtgata gcagtggttt caggtacttc tcctaatata tctcctggtt
    11461 ctacttcttc tcctacttta actaagggat gaaaatccca ttttttagag cgatccagag
    11521 gaggaacttc tactcctcgg gcaataaaat caccagaaat ttctcgaata acttccaggg
    11581 gtctctgtgt tccatcaaaa atgttaccta tcaaccccgg gcctagctcc acgctcagcg
    11641 gttcataagt tgattccacc ggctccccgg gtcggattcc cgctgtttct tcgtaaacct
    11701 gaatggtggc tttatcctgg tcaagaccaa taatctctcc caccagtttc ttttctccca
    11761 cttttaccag gtcatacatc tttgccccgg gaagtcctat cgctacgacc actggtccat
    11821 tgacactcaa tattgtacct ttagcgcttc cctccaacat catcattcct ctctctataa
    11881 agttatatcg ctcccggccg ctattcgcac cagctccctt atgaactcag atcctttccc
    11941 tatttcttcc ttccgggtag gaatgcctaa aataaccggc aaggcctcgg agcgctggtc
    12001 ttcaaaatga tccttaatca aagaataaaa cttccactcg gtgaaaatca gggcataatt
    12061 gcttctccgc aacaggggta aaaacccctt tgcttcttct gcggagtagg ctacataaac
    12121 atcaaatccc atgcctcgaa aacagaggct attttcttct cctcctacaa aagccaaacg
    12181 actatcactc atagtaacac tctcgcaatt tactttttat tttttcgctc tctaaaccaa
    12241 aatattttcc ttc
//
LOCUS       DAOPSS010000040        12141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4426_length_12141_cov_8.277097, whole genome shotgun sequence.
ACCESSION   DAOPSS010000040 DAOPSS010000000
VERSION     DAOPSS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4426_length_12141_cov_8.277097"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKN31_03740"
     CDS             complement(<1..436)
                     /locus_tag="PKN31_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99073.1"
                     /translation="MRKFLLASGLVFVFCLVMALASVGFAQVLPPGVSRENTLILPYL
                     FAPSPVPGNWNLWAGWRAQNGGLHQFVTEALWTLNPNVVEGGIINGLAAEPPIYNEDF
                     TQLTIKLRDGIYWSDGVPFTAQDLVFTILKEKDTPGLVDHGLM"
     gene            complement(626..1660)
                     /locus_tag="PKN31_03745"
     CDS             complement(626..1660)
                     /locus_tag="PKN31_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99074.1"
                     /translation="MGSGREGQKANEVCGVLKVECLKAFYVLEILGEKRIIQAVNEID
                     LEVSPNVIYGIAGESGCGKTTLLKTLFASIDPPLRLFEGKVYYQTNGKYIDALSLSPR
                     EKRALRWTYISYIPQGSMSVFNPVKKIKTTFLDVLKSHVQEKVTQDLLLLATKHLKEL
                     GLSPQVLDAYPHQLSGGMRQRVAIALATLLNPRIILADEPTTALDVVAQRAVLQLLQD
                     IQKELQNTIILVTHDMGIHAQVTTHLAIMYAGRLVEEGPTEEIFARPLHPYTRYLINS
                     LPQIGEKNRKESIPGSPPSLAFPPPGCSFHPRCSQVFGQCTVEVPALQEIYPQHKVAC
                     FLWREDHNFL"
     gene            2055..2285
                     /locus_tag="PKN31_03750"
     CDS             2055..2285
                     /locus_tag="PKN31_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99075.1"
                     /translation="MRRIRRQGAKVKKIHDTSKILCQRLLESLDIPEEVKAQLQKAFN
                     EINPAALKREIDRLLKELTKAYLKKRVEPRTN"
     gene            2716..3804
                     /locus_tag="PKN31_03755"
     CDS             2716..3804
                     /locus_tag="PKN31_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOA99076.1"
                     /translation="MPHRITLIPGDGVGPEITAVTKKVIEATGVEIEWDIQEAGAEVM
                     EKYGTPLPPSVIESIKKNRVALKGPITTPVGSGFRSVNVALRKELDLYACIRPCKTYP
                     GVRSPYQNINLVIIRENTEDLYAGIEFEKGKPETLELIEAIEKWGKGKIRPDSGISIK
                     PISVSGSERIVKFAFEYARSEKRKKVTAVHKANIMKYSDGLFLAVAREVAKNYPDIEF
                     EDRIVDNMSMQLVQKPELYDVLVLPNLYGDIISDLGAGLIGGLGVAPGANIGDEYAVF
                     EPTHGSAPKYRGMNKVNPLAMILSGMMMLKYIGEKEKAEKLEIAIKEVIKEGKKVTYD
                     LKPTPADPTACGTREVGEAIIEKLRSFS"
     gene            3829..5238
                     /locus_tag="PKN31_03760"
     CDS             3829..5238
                     /locus_tag="PKN31_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA99077.1"
                     /translation="MNILLVNPPSPGIYSWIGLKLPPLGLAYLASTLEQENHRVKILD
                     LQVAPKKELKENLKNCDLVGITCETNKAYEALKIAESAKKEGRLVVLGGYHATFCDEE
                     LLNSGLVDYIVRGEGEYIFPQLVKVLENNESPHSVLGISFREEGKIVRTPPAPLPHNL
                     DEIPFPQRELLPLSKYWMTQVEGEPLINMVTSRGCPFGCSFCASSQFSGKKWRARSPE
                     NIMEELRQVYFDLGYRAVAFVDDNFTLSPARAESICDKIEREKMKIKWWCFSRADTIV
                     KHESLVEKMARAGLRMVYLGFESAEESILQDYGKNLTAEESKKAVEILRKYGVQSWGS
                     FILGNVRETKETIKKTVEFAKQINPDIVQFSILTPFPGTETYEKYQKEGRIINRDWKA
                     FDGAHSVLQLDFLNPQELSRLAIRAYLEFYKRWSYLPQAFSFIKKFLVINLPPISRRK
                     EAWYQKERLKNANSPIPSY"
     gene            5210..5971
                     /locus_tag="PKN31_03765"
     CDS             5210..5971
                     /locus_tag="PKN31_03765"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOA99078.1"
                     /translation="MQIARFLHTEGKAPLFGVIKDDVIQEISDPFGEIKEGRGIFPRE
                     KVKLLSPTLPSKIVAVGLNYRHHAQELKQQIPENPIIFLKPSTAALSPHDFILLPSSS
                     HQVDYEGELAVIIKKKAHKVKVSQVEEYILGYTCFNDVTARDLQKKDGQWTRAKSFDT
                     FAPFGPWIETEIEPGNLNIRTYVNGHKKQDSNTRELLFPVPVLISFISEVMTLLPGDV
                     IATGTPEGVGPLHPGDIVEVEIENIGILRNPVKGE"
     gene            5974..6876
                     /locus_tag="PKN31_03770"
     CDS             5974..6876
                     /locus_tag="PKN31_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOA99079.1"
                     /translation="MYQVLAVMFLLIAVGMFLRVAKVFPPETVPLLNRFVLYLTFPCL
                     IFRSLQPAQLTQELWKIPPLAYTLISLVFLASYFLSSRLLHLKDETSVSLAMGAAFGN
                     TAFLGYPFILALLGEKGLPPAIFFDQMGNFLSTYTVGVLLCIYIQTKHLSARSLLEII
                     KLPPFLAFLLAIITNGLSIPPFLWEAINHLANATVPLTMVAIGMSLSASHLFRNWKPL
                     LLASSIKLAFLPLLFLACTKFISFSPLYQKVMVIQAATPTLMTSYTLASIYELDSEFS
                     SSVICFTTFLSLFTLPLWGLFLHP"
     gene            complement(6859..7896)
                     /locus_tag="PKN31_03775"
     CDS             complement(6859..7896)
                     /locus_tag="PKN31_03775"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HOA99080.1"
                     /translation="MDWGSWLLIFSIIILAGVIAYLGDILGRRIGKKKLSLFKLRPRY
                     TAILVSVITGILIAAITLTILSVASQDVRTALFGMKELKDQIEQLEQREKELQSSKAV
                     LEEQVKILTGSVEELKSQRQSLEEEIQVLQIELTRLRESILAIRRGEIVFQDEEEILR
                     AIAPPGMKDEEAENYLLSLIRQGEDIAIERGAGLDKEIQRAVFVLEDNFNETKERLKN
                     SEVPLVIRLVASINTLRGEPVIARFIAEENKKIFSAGEVIWSGEIDIEPQKTNIELWL
                     GELLRELNALAVKKGILPQQGRVGVISASNITEVTSLLQKMEGKVTVEVVAESDIFTA
                     GPLRIYLRVKK"
     gene            complement(7909..8991)
                     /locus_tag="PKN31_03780"
     CDS             complement(7909..8991)
                     /locus_tag="PKN31_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOA99081.1"
                     /translation="MKVLDKYILKQSVANFFFGVILFVILVSAGDLLFRLAHLWLQEG
                     FSIGEVIKIFFLSLPSLLIYILPVSVLLSVLLTVNRMSSDSEIIALRASGVSFRRLTI
                     PFFLFSLWVCAGTIIIQEVCLPWASVELKEIGERVSGGELIPENTFFRDDSEPGVERM
                     FYVKMIDPEKSLLLGVVVQEYDEEGLRRIINAQEAFNQEGKWVFKEGIIYEVGEGGEI
                     ERMIRFEKEEVATAQSMEEVQKVQKKPQEMSFLELKNFIGEERSRGRKTEKLELILWQ
                     KTAFPFASLVFVLVGVSLGIVSPRSGKALGIGLSVLIVFAYYVIFSLGSTLAEGGGLS
                     PFWGTWMANIMGFATGGGLLWWKEKN"
     gene            complement(8988..9728)
                     /gene="lptB"
                     /locus_tag="PKN31_03785"
     CDS             complement(8988..9728)
                     /gene="lptB"
                     /locus_tag="PKN31_03785"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOA99082.1"
                     /translation="MPQGKNWNALLGRGLTKEYSKKRVVNEVDLEVRRGEIVGLLGPN
                     GAGKTTTFYLLVGLVRPTEGKVFLDALELNHFPMYLRARMGISYLPQEPSVFRKLSVK
                     DNLDLVLEMQGLSKKEVKEERERLLEEFGISHLANSPANLLSGGERRRLEIARTLATF
                     PSFILLDEPFTGIDPIAVEDIQNLVKYLASKNIGVLITDHAVRETLAITDRAYIMFEG
                     KILVSGSSEEIINSDLSRKYYLGERFNL"
     gene            complement(9744..10427)
                     /locus_tag="PKN31_03790"
     CDS             complement(9744..10427)
                     /locus_tag="PKN31_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOA99083.1"
                     /translation="MRKIIFCVLAFFFFPSLLLFAQEEEVVLTITTPQAEYDAETGKI
                     VAQVSTFTWQDVKVVCPFLEIDTQTQEARSEGNISISWGDFQAQARALYYQGKENRLN
                     FLGIEGSNPEIRFSTGEAFFDFNQKRLCLTSSPLLVLQDWEVRAQNIEYSLEEKAWEA
                     HIVRVEREDWQGRAEKALYREGDEFITLEGEAEVQGDSNLLRGEKIFVYLDSGRVRVE
                     GNVEIKLVP"
     gene            complement(10424..10981)
                     /locus_tag="PKN31_03795"
     CDS             complement(10424..10981)
                     /locus_tag="PKN31_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99084.1"
                     /translation="MSHRIDWGFATLIFFSLVLLVGVIYFWWGGEAPPSPPPEIIEEE
                     YFSQIQMEKAEISRLGEKGKEWVLNAHSLDQKGDSVFLIDVSGTFFQEGAPLYQVKAQ
                     KGQVFLPEGDVELEEVKLINEERKESLQGELLLWRGKEEKFELRKAQFAGQGIEANCQ
                     LVVYNIPEEKLWLENNVELKIKVGE"
     gene            complement(10965..11738)
                     /locus_tag="PKN31_03800"
     CDS             complement(10965..11738)
                     /locus_tag="PKN31_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOA99085.1"
                     /translation="MKERKKIFLTVVVVSLLLFCGVHSLGAREAPVFQLPGLDGEIYS
                     LSDFRGEPVVISFFATWCAPCRQELPLLQKIYEEYEEKALLNVLVIDSGEPQNKVETF
                     VEELGLTFPVLLDEEGKVVRDYGVLYLPTLYFVDPLGNLAGQVIGAQEESVLRGKLDQ
                     ILWFRGLREAEVKNLVELTSEVVILDFRKESANPFPDRKNISYQVVDGVDSVISSFPR
                     EGVYLLLTENNVEGLETGKALAISGFQRVYYWMVDVPQD"
     gene            complement(11876..>12141)
                     /gene="csrA"
                     /locus_tag="PKN31_03805"
     CDS             complement(11876..>12141)
                     /gene="csrA"
                     /locus_tag="PKN31_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOA99086.1"
                     /translation="KLTKESFMLVVTRRIDQSIRIGNNIEIKILGIEKGKVKVGVSAP
                     PDIPVYREELYQSLVQDNQKALFVSEEMVSTLRKVFSPPESAD"
BASE COUNT         3675 a         2811 c         2213 g         3442 t
ORIGIN      
        1 gcatcaaacc gtggtctacg agacccggag tatctttctc tttcagaatg gtaaaaacta
       61 aatcctgggc agtaaatggt actccatcac tccagtaaat tccgtctctc aattttatgg
      121 taagctgagt aaaatcttca ttataaatag gaggttcagc tgccagcccg ttaatgattc
      181 caccttcgac cacattggga ttgagagtcc agagtgcttc ggtaacaaat tgatgcaacc
      241 cgccattctg tgctctccac ccagcccata ggttccagtt ccctggcacg gggctaggag
      301 caaagaggta gggaagaatt aaagtgtttt cccgggaaac ccccggaggg agaacctggg
      361 caaagcctac tgaagcgagt gccattacca gacaaaacac aaacaccaaa ccgctcgcta
      421 aaagaaattt acgcattttt gtatcctcct tttttaaaac caatctctaa attttccctg
      481 aaatatctga caagtgctaa acttcttgac tatgataaaa ttattttctt tttttgctca
      541 cctccttatc tttcaaacac ttttcctgaa ttctttcagt tactatctcc agattacctc
      601 ggcacagata gataagactt gagctttata aaaaattatg gtcttccctc cacaaaaaac
      661 acgccacttt gtgctggggg tagatttcct gcagcgcagg tacctctaca gtgcattgac
      721 cgaatacttg agaacaccgg ggatgaaaag aacaaccggg gggaggaaat gcaagagaag
      781 gaggactccc cgggatactt tctttacgat ttttttctcc aatctggggc aaagaattaa
      841 tgagatatcg ggtatagggg tgaaggggcc gggcgaaaat ttcttcggta ggtccttctt
      901 ctactaacct tccggcgtac atgatagcca ggtgagtggt aacctgggca tgaataccca
      961 tatcatgggt gacaagaata atagtatttt ggagctcttt ttgtatatcc tgtaaaagtt
     1021 gtagaactgc tctttgggct accacatcca gagcagtggt gggctcatca gctagaatga
     1081 tgcggggatt gagaagggta gccagagcaa ttgctacccg ttgtctcata cctccagaaa
     1141 gctggtgagg ataggcatcc agaacctggg gagaaagacc taattccttg aggtgttttg
     1201 tagcgagcaa aagaaggtcc tgggtaactt tttcttggac atgacttttg agcacatcga
     1261 gaaaagttgt tttgattttc ttcacagggt taaaaacact catcgaaccc tggggtatat
     1321 aagagatata ggtccagcgc aaagccctct tttcacgggg acttaaagaa agggcatcta
     1381 tgtacttccc attggtttgg taataaactt tcccttcaaa aagccgcagg ggagggtcaa
     1441 tggaagcaaa gagggttttc agaagggtgg ttttcccgca gccgctttct ccagcgatac
     1501 cataaatcac attgggagaa acctctaaat caatttcatt aaccgcctga ataatccttt
     1561 tttctcccag tatttcaagg acatagaaag ccttgaggca ctctactttc agtactccgc
     1621 atacctcatt tgctttttgt ccttctctcc cactacccaa gacaaaaacg tctccttccc
     1681 ctcaccggtg gtaaagtcaa aatctccata caaaaatggc aaatttttct ctgccacaaa
     1741 ttgcaggcat ttaagagtat agagatttcc aaactaaaag aagtagtcgg agtttaaggc
     1801 aaactatacc atagtccttg ttaaaaaaag aaagggaatc aatatgctcg gtctttaagt
     1861 gtcctcttgt tcagtataaa agctcccgct ctccggaaac atccttctta aataaccgcc
     1921 caaattctaa caaaaacagc ccgaacctgt caatcattcg agttgcctca tttaaaatca
     1981 aagtgaattt ccctatcaaa ggtctgaact cagataatga tagtaccttt atcaactacc
     2041 aatgttttct tactgtgagg agaataagaa gacagggagc taaggttaag aaaatacatg
     2101 atacttccaa gattctctgt caaagactat tagaatctct ggatatacct gaggaggtaa
     2161 aagcacaact gcaaaaggct ttcaatgaaa ttaatccagc tgccttaaaa cgagagatag
     2221 ataggttact gaaagaatta accaaagctt atctcaagaa gagggtagag cccagaacaa
     2281 attgacagag aacttatttt cacctagaat tttatatgag acatcaggga agtaagtggc
     2341 tctatttgtg gctattccag aataatatca aacaacctgc ggcgcaaaat agaaatgaaa
     2401 aagataaaaa cgttgagtaa cagaatcaac tttttattgt ttgtagaatc ttgttaccgc
     2461 aggcctaaaa agccctgaat ttccattaac tactgagaga aaaaagagaa tttagactgt
     2521 gaatgaggaa agaaactcta taaaaattgt caataaagtg cgttctttgt ttacctttgc
     2581 ctgggtaaat ataaagagaa aaaaccataa agggagaata gtatctagaa aacttcttcc
     2641 cttggatttt gtgatagaat aaatagtaaa gtaaatagat tactacagta aacaattgga
     2701 aaggaaggag aaaaaatgcc tcatcgtata actttaatac caggagatgg tgttggacca
     2761 gaaataactg ctgtaactaa aaaagtgatt gaagccacag gagtagaaat agaatgggat
     2821 atccaggaag caggagcaga agtaatggaa aaatatggca ctccccttcc tccttcggta
     2881 attgaatcta taaagaaaaa cagagtagct ctgaaagggc ccatcactac tccggtggga
     2941 agcgggtttc gtagcgtcaa cgtagcctta cgaaaagagt tagaccttta tgcctgtatt
     3001 cgtccctgca aaacctatcc cggagttcgc tctccttatc aaaatatcaa tttagtaatt
     3061 atccgagaaa acaccgaaga cttatacgcc ggcatagaat tcgaaaaagg taaaccagaa
     3121 actctggagc tcatagaagc aatagagaaa tgggggaaag gtaaaatccg tcccgactcc
     3181 ggcatcagta ttaaacctat ctccgtttct ggttcagaga gaatcgtaaa atttgctttt
     3241 gaatatgctc gttctgaaaa acggaaaaaa gtcaccgccg ttcacaaggc taacattatg
     3301 aaatactccg atggcctatt tttagccgtc gctcgagaag tggctaaaaa ctatcctgac
     3361 atagaatttg aggaccgcat agtggataat atgagtatgc agctggtgca gaaacccgag
     3421 ctttacgatg ttttagtttt acccaatctt tatggagata taatatctga cctgggagca
     3481 ggattgatag gggggctggg tgtggctccg ggagctaaca tcggtgatga atatgcggtc
     3541 tttgagccta cccatggcag cgctcctaaa tatcgaggaa tgaacaaagt taacccctta
     3601 gccatgattc tctcgggaat gatgatgtta aaatatatag gagaaaaaga aaaggcggaa
     3661 aaattagaaa tagcaataaa agaagtgata aaagaaggta aaaaagttac ttatgattta
     3721 aaacccactc ctgctgaccc cactgcttgt ggaactcggg aagtaggaga ggccatcatc
     3781 gagaaactaa gaagtttttc ttaaaaggaa ccaaagaggt gaaagctgtt gaacattctt
     3841 ttagtaaatc ccccatctcc gggaatttat agctggattg gtctaaagtt accccctctg
     3901 gggttagcgt atttagcaag cactttagag caggagaatc atcgggtaaa aattttagac
     3961 cttcaagtag caccaaaaaa ggaattgaag gagaatctga aaaattgcga tttagtagga
     4021 ataacatgcg aaaccaacaa agcttatgaa gcactgaaaa tagccgaatc agccaagaaa
     4081 gaaggacgcc tggtagtgct gggaggatat cacgccactt tttgtgatga ggagttatta
     4141 aatagtggct tagtagatta catagtgcga ggagagggag agtatatttt tccccagtta
     4201 gttaaggttc tggaaaataa tgaatctcct cattctgttt taggaatttc tttccgggaa
     4261 gaaggaaaaa ttgtccgcac tcctcctgct cctcttcccc acaaccttga tgaaattcct
     4321 ttccctcaac gagagcttct acctctttct aaatactgga tgacccaggt agaaggagag
     4381 cccttaataa acatggtaac cagccggggt tgcccttttg gctgctcttt ctgtgcttca
     4441 tctcagtttt cgggtaaaaa gtggagagct cgttctccag agaatataat ggaagagctg
     4501 cgccaggttt actttgactt aggatatcga gcagtagctt tcgtggacga taattttacc
     4561 ctgtcccctg ctcgggcaga aagtatttgc gacaaaatag agcgggaaaa aatgaaaatc
     4621 aaatggtggt gcttttcccg agctgacacc attgtaaaac acgaatcctt agtagaaaag
     4681 atggccagag caggcttgcg gatggtctac ttagggtttg aaagcgctga ggaatccatt
     4741 ctgcaagatt acgggaaaaa tttaaccgct gaggaatcta aaaaagcagt ggaaatcttg
     4801 cgtaaatacg gagttcaaag ctggggttct ttcatattgg gtaatgtcag ggaaactaaa
     4861 gaaactatca aaaagactgt ggaatttgcc aaacaaatta atccagacat tgtacaattt
     4921 tccattctca ctcctttccc cggtactgaa acatacgaaa aatatcagaa agaaggacgc
     4981 atcatcaatc gcgactggaa agcatttgat ggagcacatt ccgttctaca actggatttt
     5041 ctcaatcctc aagaattaag ccgtcttgct atccgagcat atcttgaatt ttacaagcgg
     5101 tggagttatc ttccccaggc tttcagtttc ataaagaaat ttttagtgat caatttacct
     5161 cctatttctc gacgcaaaga agcctggtat cagaaggagc gattaaaaaa tgcaaatagc
     5221 ccgattcctt catactgaag gaaaagctcc tctttttgga gtgataaaag atgatgtgat
     5281 tcaagaaatc tctgaccctt ttggagagat aaaagaggga agaggaatct tccctcgaga
     5341 aaaagttaaa ttgctttctc ccaccctacc ttctaaaatc gtagcagtag ggttaaatta
     5401 ccgccaccac gctcaggagt taaaacagca aatccccgaa aaccctatca tttttcttaa
     5461 accctccacc gccgctcttt ctcctcacga tttcatcctc cttccctctt cttctcatca
     5521 agtagactac gaaggagaac tggcagtaat aataaagaaa aaagctcata aagtgaaagt
     5581 ttcacaagtt gaggaataca ttttaggata cacttgcttt aatgatgtca ctgcccgaga
     5641 tttacaaaag aaagatggac aatggacgag ggctaaatct tttgatactt ttgctccttt
     5701 tggcccctgg atagaaaccg aaatagagcc gggaaatttg aatattcgca cctatgtcaa
     5761 cggacataaa aaacaagact ctaacaccag agaactctta tttcctgtgc ccgtgcttat
     5821 ctctttcatc tccgaagtca tgactttgtt gccgggagat gttattgcta ccgggactcc
     5881 tgaaggagtg ggaccactgc accccggaga tatagtagaa gtagagatag aaaatatcgg
     5941 aatacttcgc aatccagtaa aaggagaata agcttgtacc aagttctggc ggtaatgttt
     6001 cttttaatcg ccgtggggat gtttctgcga gtagcaaaag ttttcccccc cgaaactgtt
     6061 cctcttttga atcgttttgt tctctatctt acttttccct gtcttatatt tcgctcgctg
     6121 cagcctgctc aattaactca ggaactgtgg aagattcctc cccttgccta taccttaatc
     6181 agtctggttt tccttgcctc ttatttttta tcctcccggc ttttacactt aaaagacgaa
     6241 accagtgtaa gcttagctat gggagcagct tttggtaaca ccgctttttt aggatatcct
     6301 ttcattctgg cccttttagg agagaagggc ctaccacctg ctatattttt tgaccagatg
     6361 gggaactttc tctctaccta taccgtagga gtgttgctct gcatatatat ccagaccaaa
     6421 cacctatcag cccggagtct tttagaaata ataaagcttc cacctttttt agcgttttta
     6481 ctggctatta taaccaacgg tctatccatt cctccttttc tctgggaggc cattaatcac
     6541 ctcgctaacg ccactgttcc cctgactatg gtagctatag gaatgtccct ttctgcttct
     6601 catctcttcc ggaattggaa gcctcttctt ttagcttcct ctatcaaatt agcttttctt
     6661 ccccttttgt ttttagcatg tactaaattt atctccttca gcccccttta ccaaaaagtc
     6721 atggttattc aggccgccac tcccaccctt atgacttctt atacgctggc ttccatttat
     6781 gaattagata gtgaattctc ttccagcgtt atctgtttca ccactttttt atcgcttttc
     6841 accctgccct tgtggggtct atttcttcac ccgtaagtaa atacgcaaag gtccagcagt
     6901 aaatatgtcg ctttctgcca ccacctccac tgttaccttt ccttccattt tctgcaaaag
     6961 agaggtaact tcagtaatat tggaagcaga aataactccc actctgccct gttgaggaag
     7021 aatccctttt tttaccgcta aggcattgag ttcccttaac aattccccca accagagctc
     7081 tatattagtt ttttgaggtt caatgtctat ctctccagac catatcactt ccccggcaga
     7141 aaatatcttt ttattctctt ctgctataaa tcgagcaata actggttctc ctcgcagagt
     7201 attgatggaa gccaccaaac gaataactaa tggaacctcg ctatttttta atctctcttt
     7261 agtttcatta aagttatctt ctaaaacaaa aacagctctt tgtatttctt tatcaagacc
     7321 tgctcctcgc tctatggcga tatcctcccc ttgcctgatc agagaaagaa gataattctc
     7381 tgcctcttca tctttcatac ccggaggggc tatcgcccgc aaaatttcct cttcatcctg
     7441 aaacactatt tctccccgcc ggatagccaa aatactttcc cgcagacgag tcagctctat
     7501 ttgtaaaacc tgtatctctt cctcgagact ctgcctttga ctctttagct cctccacgct
     7561 tccagttaaa attttaacct gctcttccag cacagcttta gagctctgca actccttttc
     7621 tctctgttcc aattgttcaa tttgatcttt tagctctttc ataccaaaca gagccgtacg
     7681 cacatcctga gaggcaacgc ttaaaatagt aagagtaata gcagcgatta aaatcccggt
     7741 aataacactc accagaatag cagtgtagcg cgggcgaagt ttaaacaaag aaagcttttt
     7801 ctttccaatt cttcttccta aaatatcacc caagtaagct ataactccag ccaaaatgat
     7861 tatagaaaaa atcaacaacc agctacccca atccatagaa agtctccttc agtttttttc
     7921 tttccaccac aacaggcctc caccagttgc aaaacccatt atattagcca tccaggttcc
     7981 ccaaaaggga gataagcccc ctccttctgc cagagtacta cctaaagaaa aaataacgta
     8041 ataagcaaaa actataagaa cactcaatcc tattcctaaa gctttgcccg aacgaggaga
     8101 aacgataccc agagaaactc ctacaaggac gaaaaccaga ctggcaaagg gaaaggcggt
     8161 tttttgccac agaattaatt ctaacttctc cgttttcctt cctctacttc tctcttcccc
     8221 gatgaaattc ttcaattcca gaaaactcat ttcctgcggc tttttctgga ctttctgtac
     8281 ctcttccatg ctctgagcag tagcaacttc ttccttttca aaacgtatca ttctttctat
     8341 ctctccccct tctcccacct catatataat tccttcctta aatacccact tcccttcctg
     8401 attaaaggct tcctgggcat tgatgattct cctcagaccc tcttcgtcat attcttgaac
     8461 cactactccc aaaaggagag atttctccgg atctatcatt tttacataaa acatcctctc
     8521 tacgcctggt tcactgtcgt cccggaaaaa agtattttct ggtattagtt ctcctccact
     8581 cactctttct cctatctctt taagctctac tgaagcccag ggtaaacata cctcctggat
     8641 gattatagtc ccggcacaaa cccacagaga aaaaaggaaa aaaggaatag tcagccttct
     8701 aaaactaacc ccacttgctc tcaaggctat aatttcacta tcgctggaca tgcggttcac
     8761 ggtaagcagc acacttaaca ataccgaaac cggcaaaata tagataagaa gggagggaag
     8821 actcaaaaaa aatattttta taacttcccc tattgaaaaa ccttcttgca accacaagtg
     8881 agccagacga aagagcaggt ctccagcgct tactaaaata acgaacaaaa tgactccaaa
     8941 aaaaaagtta gctacgctct gcttgagaat atatttatct aatactttca aagattaaaa
     9001 cgttctccca aatagtattt tcggctcaaa tcggaattaa taatttcctc cgaactgcca
     9061 gaaactaaaa ttttaccttc aaacataata taagcccggt cggtgatagc cagggtttct
     9121 cgtacagcgt gatcggtaat caaaactccg atatttttac tggccaaata cttcaccaaa
     9181 ttttgaatgt cctcaactgc aatagggtca atcccggtaa atggctcatc taaaaggata
     9241 aaggagggaa aagtagccag tgttcgggca atttctaaac gtcttctttc tcctccggag
     9301 agtaaattgg ccggggaatt agccagatga ctgatgccaa actcttccag caggcgctct
     9361 ctctcttctt tcacttcctt tttagatagg ccctgcattt ctaaaaccag atctaagtta
     9421 tctttcactg aaagttttcg gaaaacagaa ggctcctgag gaagataact tatacccata
     9481 cgagctcgta aatacatggg aaagtgattg agttccaggg catctaaaaa aactttccct
     9541 tccgtgggtc ttacaagacc caccagaaga taaaaagtag tggtctttcc tgctccattg
     9601 ggtcctaaaa gaccaactat ctctcctctt cgcacctcta aatcaacttc atttactact
     9661 ctttttttac tatactcctt ggtcaagcct ctccctaaca aggcattcca gtttttccct
     9721 tgaggcaagc gcatttcctc cttttaagga accagtttta tctccacgtt cccttccact
     9781 ctcactcgac cactatccag gtatacaaat atcttctctc ctcgcaaaag attactatct
     9841 ccttgcacct ctgcttcgcc ttctagagtt ataaattcgt ctccttctcg ataaagagcc
     9901 ttctctgccc taccctgcca atcttctctc tctactctta ctatatgagc ctcccaagct
     9961 ttctcctcca gagagtattc tatattctga gccctgacct cccaatcttg caagaccaaa
    10021 agaggagacg aggtaagaca gagccttttt tgattaaaat caaaaaaggc ttccccggta
    10081 gaaaagcgta tttctggatt agagccctct attcccaaaa aatttaatcg gttttcttta
    10141 ccctgataat agagagctcg ggcttgagct tgaaaatcgc cccaggaaat acttatattt
    10201 ccttcacttc tcgcttcttg agtttgagta tctatctcca aaaaagggca caccactttt
    10261 acatcttgcc aggtaaaggt agaaacttga gctacgattt tcccggtctc agcatcatac
    10321 tcagcttgag gagtagtaat agttaatacc acttcctctt cttgagcaaa aagaagaagg
    10381 gaaggaaaaa agaaaaaagc cagaacgcag aagataattt ttctcattct cccaccttga
    10441 tttttaattc tacattgttc tccaaccata atttctcctc cggaatatta taaactacta
    10501 attgacaatt agcttctatt ccttgcccgg caaactgagc ctttcttagt tcaaattttt
    10561 cttcctttcc ccgccacaac aatagctctc cttgtaaact ttccttcctc tcttcattta
    10621 tcaattttac ctcttccagt tccacatccc cttccggtaa aaatacttgc cctttttgag
    10681 ctttcacctg atagagagga gctccttcct ggaaaaaagt tcccgaaaca tcgattaaaa
    10741 aaacgctatc ccccttctgg tctagcgaat gagcattcag aacccattct ttacccttct
    10801 ctcccagccg gcttatttct gctttttcca tctggatttg agaaaaatat tcctcctcga
    10861 ttatttctgg tgggggagag ggaggtgcct ctccccccca ccagaaataa attacaccta
    10921 ccagtaaaac aagagaaaag aaaattaagg ttgcaaaacc ccaatcaatc ctgtgggaca
    10981 tctaccatcc agtaataaac ccgttgaaaa ccagagatag ctaaagcttt gcctgtctcc
    11041 aggccttcaa cattattttc ggtaagtaaa aggtaaaccc cttctcgagg aaaagaagaa
    11101 ataacggaat ctacaccatc caccacctga taagaaatat tttttctatc gggaaaagga
    11161 ttagcactct cttttctaaa gtctaaaatc actacctctg aagttaactc cactaaattt
    11221 tttacttctg cctcccggag gcctcgaaac cagagaattt gatccagttt accacgcaaa
    11281 acactctctt cctgggctcc aattacctga ccggctaaat tgcccagagg atccacaaaa
    11341 taaagagtag gtaaatataa aacaccgtaa tcacgaacca ctttcccttc ctcatctaaa
    11401 agaacaggaa aagtaagacc cagctcttct acaaaagttt ctaccttatt ttgaggctct
    11461 cctgagtcta taactaaaac atttagcagg gctttttctt catattcctc gtatattttt
    11521 tgtaaaagag gaagttcctg acgacagggc gcacaccagg tagcgaaaaa agaaattacc
    11581 accggctctc ctctaaaatc ggagagggaa tagatttctc catctaaccc cggaagttgg
    11641 aacacaggtg cttcgcgagc ccccagagaa tgaacgccac aaaacaacaa aagagaaacc
    11701 actactacag tcaaaaagat cttttttctc tctttcaagc tttttcctcc tcacaccttt
    11761 attttttata tatcgattcc tttcctcaaa aattcccctg aaataattat aagaatctat
    11821 ttctgaaaac gatagtagat aacagccata aataggagaa atataccaga aacgctcaat
    11881 cggcagattc aggaggagag aaaacctttc gaagagtgga aaccatttct tcagaaacaa
    11941 agagggcttt ttggttatct tgcacgaggg attgatataa ctcttctcgg taaaccggga
    12001 tatcaggagg cgcagacact cccactttta ctttcccctt ttctataccc agaattttta
    12061 tttctatgtt attgccaatt cgaatgcttt gatctatcct ccgggtaaca accagcataa
    12121 aactctcctt agtaagtttt a
//
LOCUS       DAOPSS010000041        12129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4437_length_12129_cov_8.176826, whole genome shotgun sequence.
ACCESSION   DAOPSS010000041 DAOPSS010000000
VERSION     DAOPSS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4437_length_12129_cov_8.176826"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKN31_03810"
     CDS             complement(<1..652)
                     /locus_tag="PKN31_03810"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOA99087.1"
                     /translation="MKKLILPFLILILFLQLSPAFSKSYFHPSINQSFVLKENGDVEV
                     EEERYFSFSGSFSWAQLTIYLQGVEDIDFSGVWSIETGEPLSFQVEEGANNKSLKWFY
                     QAENETRGFKIKYILRQAVKRYQDVAEFYWKVLEDEHAEVKNLQTSFFLPQASPELLK
                     LFVHTKAEPGEIVFSPDYKRVDFYLSSIPSDAFVEARLLLEPHIFPLLPLLAENRYE"
     gene            729..1442
                     /locus_tag="PKN31_03815"
     CDS             729..1442
                     /locus_tag="PKN31_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOA99088.1"
                     /translation="MQKFLGAFFTLPGILVTVPLCLFLRSLIKKKATWSFLFLALLTY
                     FFSIGGGLYLLPREAPSRDATATPQAIVVLGGGQVSSFLRTYQAFLLWEKEQIPLILS
                     GGKIEKGGKTSEAEIMKELLLSWKVKEEYIYLEPASRTTWENALYTTEILREKGWQNF
                     YLVTSEVHLKRACFSFHHLYPEAHIVPVSSNPVYNFSTFSWMDFLPSGEAFSAWIEII
                     HEYAGYLFYRIVYGVSSST"
     gene            complement(1418..2326)
                     /locus_tag="PKN31_03820"
     CDS             complement(1418..2326)
                     /locus_tag="PKN31_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA99089.1"
                     /translation="MDKKYLSHPYFLLILVSLIWGFNFSVVKMGIMAIGPLAFSFLRF
                     ILSALLMFFVLYITEKNPWVEVKDLPYFLFLGLLGFGIYQPLWSYGLKLSLASHSALI
                     LSLSPVVVSLVAFLRKEELVGGINLLGVIVGFGGVMFLVSQGGSGDYSSESVLYGDLL
                     TLGAAISWGLYSYFGKNMVKKYSPLKTSTWSILWGLMFMFPICIGETLQIRAEDFSFS
                     LLLSLSYSVVLSSLVAYIIWMQCVREIGATRTVSSQYITQVVGVIAAWMIFGEPLGWQ
                     VFLGMAFISLGLWLVNRKPQVEELTP"
     gene            complement(2363..2740)
                     /gene="rplL"
                     /locus_tag="PKN31_03825"
     CDS             complement(2363..2740)
                     /gene="rplL"
                     /locus_tag="PKN31_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOA99090.1"
                     /translation="MTKEEIIESIEKMTVLELAELVKALEEKFGVSAAMPVAQMAAAG
                     TQGAAAPEVEEKTEFDVVLKGVGDQKLQVIKEVRAITGLGLKEAKELVDNAPRPVKEG
                     ISKEEAEEIRAKLEAVGAEIEVK"
     gene            complement(2743..3273)
                     /gene="rplJ"
                     /locus_tag="PKN31_03830"
     CDS             complement(2743..3273)
                     /gene="rplJ"
                     /locus_tag="PKN31_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOA99091.1"
                     /translation="MQKVDKEKIWQEVYEKLQKSEAVFVVNYQGIKVESMNELRRSLK
                     NAQGEIKVVKNTLARIALDKAGIPYDDELMRGQNAFAFSYLDAVQIAKLLADAGKKLP
                     QLEVKGGWLNRNPLKPEEIKRLAQLPSREQLIAQVVGGMKAPITGLVGVLQGLLRSLV
                     GVLQAIEEKKNAQKED"
     gene            complement(3465..4181)
                     /gene="rplA"
                     /locus_tag="PKN31_03835"
     CDS             complement(3465..4181)
                     /gene="rplA"
                     /locus_tag="PKN31_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOA99092.1"
                     /translation="MADRGKRYLTAKNNIEAGKLYDPETAIRLVKETANARFDETIEV
                     AINLGIDPKQSDQQVRGAVSLPHGVGKKVRVLVFAQGEKAREAQEAGADYVGGEELAD
                     KIQSGWLEFDAAVATPDMMRVVGKLGKVLGPRGLMPNAKTGTVTFDVAEAVKEIKAGR
                     IEIRNDRYGNVHAPIGKASFPVEHLLDNFCALLDAVVRLKPPASRGRYIKKIVISSTM
                     GPGIPVDPKLALESLEKRVA"
     gene            complement(4198..4626)
                     /gene="rplK"
                     /locus_tag="PKN31_03840"
     CDS             complement(4198..4626)
                     /gene="rplK"
                     /locus_tag="PKN31_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOA99093.1"
                     /translation="MAKKIIAVVKLQIPGGMATPAPPVGPALGQHGVNIMEFCKAFNA
                     ATEKDRGVLIPVEITIYGDRSFTFICKTPPASFLIKQAVGVEKGSGEPNRVKVGKIPR
                     SKIREIAEKKMPDLNASDLEGAVKIIEGTARSMGVEVIEG"
     gene            complement(4645..5169)
                     /gene="nusG"
                     /locus_tag="PKN31_03845"
     CDS             complement(4645..5169)
                     /gene="nusG"
                     /locus_tag="PKN31_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOA99094.1"
                     /translation="MSKQWYVVHTLAGSEHKVKANLERRITSMGMQDQIFRVVVPMEE
                     AIEVKRGKKRFTKKKVFPGYVMVEMIMNDRSWYVVRNTPGVTGFVGSGMKPEPLNEEE
                     VSVILRQTGVEKGVPRLDVEKGEMVKVIAGPFLNYTGTVESVDQEKGKMTVLLSIFGR
                     ETPVELDFSDVEKL"
     gene            complement(5192..5395)
                     /gene="secE"
                     /locus_tag="PKN31_03850"
     CDS             complement(5192..5395)
                     /gene="secE"
                     /locus_tag="PKN31_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOA99095.1"
                     /translation="MKRLGKWFRSLKQYFRDAWNELRKVTWPSKKELWNNTLTVLVVI
                     VLAGAFLGAVDLLLTFLVNLYLR"
     gene            complement(5509..5715)
                     /locus_tag="PKN31_03855"
     CDS             complement(5509..5715)
                     /locus_tag="PKN31_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99096.1"
                     /translation="MEEKERAREVFEEVFFKPMEKVLGPFLNEEARQHLSQARVEVLK
                     AMRSFIDAEIERVADKTEKKTSEP"
     gene            complement(5732..6133)
                     /locus_tag="PKN31_03860"
     CDS             complement(5732..6133)
                     /locus_tag="PKN31_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99097.1"
                     /translation="MAFIHSSKDKRVANLARYMVGIFEKEGWTVKVIEAEDTTSPVSL
                     RPFDLIFVGSQVLSLWGGKISQEVVNFLQGASGMEGKRAVAYVRPNLFGTDKALKKLM
                     SKMESQGAFVVDFEALKGEKEAEDLVKRHLK"
     gene            complement(6163..6969)
                     /locus_tag="PKN31_03865"
     CDS             complement(6163..6969)
                     /locus_tag="PKN31_03865"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOA99098.1"
                     /translation="MKKWFKTIITIKEEEKEKLLLQLQEQNILGVWESGFDEMIVYSE
                     NSIVLPPGVKGREEEEGEGEWAYKWRESFKPIRVGERIVVRPPWEDPQGAAVELVIYP
                     AYAFGTGQHPTTYWCLYMIDKYFQKGQSFLDVGSGSGILSILAARLEGGRIVALDIDP
                     HAGEELRRNLSLNDIDFSRVNFKEEEIEEIKENFDFIVCNIGTNFHLLHLETMNNLLN
                     EEGILVLSGGEEKDFSLLQEKAKMLSLKEKEIKVDSSWVTVAYKKSENVL"
     gene            complement(6953..7834)
                     /locus_tag="PKN31_03870"
     CDS             complement(6953..7834)
                     /locus_tag="PKN31_03870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA99099.1"
                     /translation="MKGETSHRLFWNGGVKREKKRDKNWEKRIHTDLVWLEIEPFLQP
                     GLKVLDAGGGYGRYSIPLAERGCQVTHLDLSPRMIEEAKKLAGDRGVSSIEFQVGKVQ
                     DLSSFSDRYFDLTISLDAPVSYAYPEQKKALEELGRVTKDKLIVSVVNRWGQVPVAVE
                     TELRFRNSLEISRRFFREGNWDHPSFWENLEEKIPLLYRYIFPPLHAFTPSEILDDLI
                     EEGFRPLRVVASGTLARLLPPRTLKKIVNNPSLYKEFLEFSREYDALFEVLGVGAKVA
                     SGLLVVGERRNSNEEMV"
     gene            complement(7834..9654)
                     /gene="thrS"
                     /locus_tag="PKN31_03875"
     CDS             complement(7834..9654)
                     /gene="thrS"
                     /locus_tag="PKN31_03875"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOA99100.1"
                     /translation="MNVKSKEAIAAVTEDGRLVDIGEEEKVKNYLSFADEAGREIYWH
                     STSHIMAQAVKRLFPEAKLAIGPAIEEGFYYDFDIPRPLTDEDLEEIEKEMRKIIQED
                     LPFRRKEVSKEEAQKIFAERGETYKVEILEEIEESKVTLYEQGEFLDLCRGPHVPSTG
                     YIKAFKLLSVSGAYWRGREDNPMLQRIYGISFDSEEKLQEFLKRREEAKRRDHRRLGR
                     ELDLFSLHEEGPGFPFFHPRGMVVINALLDLWRREHLKRGYQEVKTPMILERALWEQS
                     GHWEHYRDNMYFTKIDDRDFAIKPMNCPGGILIFKSQLHSYRELPLRWAELGLVHRHE
                     LSGVLHGLMRVRCFTQDDAHIFMEPHQVKQEIIGVIELADYFYRLFDFTYEVELSTRP
                     ENSMGSDEMWDLATRSLEEALRELEVDYRVNPGEGAFYGPKIDFHLRDCLGRRWQCGT
                     IQLDFAMPEKFDLYYIGEDGNRHRPVMLHRTVLGSIERFLGILIEQFAGAFPLWLAPV
                     QVVILPIAERHIDYAREIKDVIFSEEIRVEINDENATLGAKIRKAEVEKVPYLAIIGD
                     REMENRTLSIRKRKKGDIGSFTLDELLKNLKREIEEKVVD"
     gene            10271..11101
                     /locus_tag="PKN31_03880"
     CDS             10271..11101
                     /locus_tag="PKN31_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOA99101.1"
                     /translation="MEKKIAIIGCGSMGKMLLEKIINSKIIPEDNLFVANRTLEKIQV
                     LKEKYKNMHICRTNKEAAENSDIIFICVRPSDIKTVLLEIRDVLDNSKHIISLNGSIL
                     FEQIETVCKNNKISKVIPSVTAEVNESQTLVCHNSKVTKEDEIVLNKILGTFGKVIEL
                     PENEMGMGAELVSCMPGFIAAIFNEIVVVAQEHTKIPEEQITNMLLNTMMGTSKLMLE
                     NKLSFEEVIIRVATKGGITEEGTKVIHEKLPGIVKEMFEKTLEKRKLTTENAIKAFNS
                     "
     gene            complement(11666..11740)
                     /locus_tag="PKN31_03885"
     tRNA            complement(11666..11740)
                     /locus_tag="PKN31_03885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11706..11708),aa:Val,seq:gac)
     gene            complement(11754..11828)
                     /locus_tag="PKN31_03890"
     tRNA            complement(11754..11828)
                     /locus_tag="PKN31_03890"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11793..11795),aa:Cys,seq:gca)
     gene            complement(11835..11910)
                     /locus_tag="PKN31_03895"
     tRNA            complement(11835..11910)
                     /locus_tag="PKN31_03895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11875..11877),aa:Gly,seq:gcc)
     gene            complement(11917..11992)
                     /locus_tag="PKN31_03900"
     tRNA            complement(11917..11992)
                     /locus_tag="PKN31_03900"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11957..11959),aa:Phe,seq:gaa)
     gene            complement(12007..12085)
                     /locus_tag="PKN31_03905"
     tRNA            complement(12007..12085)
                     /locus_tag="PKN31_03905"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12047..12049),aa:Asp,seq:gtc)
BASE COUNT         3532 a         2995 c         2087 g         3515 t
ORIGIN      
        1 tttcatatct attttcagcc agaagaggaa gaagaggaaa aatgtgcggt tcaaggagca
       61 ggcgagcttc cacaaaagca tcagaaggaa tagaagagag ataaaaatcg actctcttgt
      121 aatcaggaga aaagactatt tcccctggct ctgcctttgt gtgaacgaaa agctttaaaa
      181 gctcgggact ggcctgagga aggaaaaaag aggtctgtaa attcttcacc tctgcatgct
      241 catcttctaa aactttccag taaaattctg ccacatcctg gtagcgtttc actgcctggc
      301 gcaagatgta ttttatttta aaacctctgg tttcattttc tgcctggtaa aaccacttga
      361 gagatttatt atttgctccc tcttctacct ggaaagaaag tggctctcca gtttctatac
      421 tccacactcc cgaaaaatcg atatcctcca ctccctggag gtatatggta agctgagccc
      481 aagaaaaact acccgaaaag gaaaagtagc gctcctcttc tacctctaca tctccgttct
      541 cctttaaaac aaaactttga ttgatggagg gatgaaaata agatttagaa aaagcagggg
      601 agagctgaag aaaaagaatt aaaattaaaa agggtaagat tagcttcttc atatttcctc
      661 cctgatatag taaactttcg ctaatatatc ataaactaag gagaaacaaa ataaaaatta
      721 tgttcttttt gcaaaagttt ttaggagctt tctttacttt accgggaata ttagtcactg
      781 tgcctctctg cttattcctg cggagcttaa taaagaaaaa agctacctgg tcgtttttat
      841 ttctggcgct attgacttat tttttctcta tcggcggggg cctttacctt ctaccccggg
      901 aagctccgtc cagagatgca accgctactc ctcaagctat agtagtattg ggaggaggac
      961 aagtttcttc ttttcttcgc acctatcagg cctttctcct gtgggaaaaa gaacaaatac
     1021 ctctaatttt aagtggagga aaaatagaaa aaggaggcaa aacttctgaa gctgaaatta
     1081 tgaaagagct actcctgagc tggaaagtaa aagaagaata catatacctt gaacccgcat
     1141 ctcgcactac ctgggaaaat gccctctaca ctacagaaat attgcgagaa aaaggctggc
     1201 aaaattttta cctggtaacc tcagaagtcc acctaaaaag agcttgcttt tctttccacc
     1261 atctctatcc cgaagctcac attgtcccgg tgagctctaa tcctgtttac aacttcagta
     1321 ctttttcctg gatggatttt ctacccagtg gagaagcttt ttccgcctgg atagagataa
     1381 tccacgaata tgccggttat ctcttttacc ggatagttta tggcgtcagt tcctctacct
     1441 gaggttttct gttaaccaac cagaggccca aactgatgaa agccatccct aaaaagacct
     1501 gccaccctaa gggttcgcca aaaatcatcc aggcagcaat tacaccgact acctgggtaa
     1561 tatattgaga agaaacagtg cgtgtcgctc caatttccct cacacactgc atccagataa
     1621 tataagctac taaagaagaa agaactacag agtaagaaag agatagtaag agagaaaaac
     1681 taaagtcttc cgctcttatc tgaagagttt cccctataca tatcggaaac ataaacatca
     1741 atccccagag gatactccaa gtagaagttt taagaggaga atattttttc accatattct
     1801 tgccaaaata agaataaagc ccccaagaga tggcagcccc caaagtcaat aagtctccat
     1861 ataaaacaga ttcagaagaa taatctccac tccctccctg gctcactaaa aacatcactc
     1921 ccccaaagcc tacaattacc cctaataaat ttattcctcc taccaactct tctttccgca
     1981 aaaaagctac taaagatacc accaccggag aaagagacaa aataagagca gaatgggaag
     2041 ccaggctaag cttaagcccg tagctccaca gaggctgata aatcccgaat cctaaaagac
     2101 ccagaaataa aaaataagga aggtctttta cctctaccca gggattcttc tcggtgatat
     2161 acagaacaaa aaacataagc aaagcactga gtataaagcg caagaaagaa aaagccaggg
     2221 gaccaatggc cataatcccc attttaacca cgctgaaatt aaatccccag atgagagaaa
     2281 ccagaattaa taaaaaataa ggatgggaga gatatttttt atccaaagca gatttaaaaa
     2341 gaggggcttt atagcccctc ttttatttaa cttctatttc tgcacctact gcttctaatt
     2401 tagccctaat ttcttctgct tcttctttgc taatcccttc tttaaccgga cgaggagcgt
     2461 tatccaccag ctctttggct tctttcaatc ctaaaccagt gatagcccgt acttctttga
     2521 taacttgaag cttctggtct cctacccctt tcagcaccac atcaaactca gttttttctt
     2581 ccacttcagg agcagcagct ccctgcgttc ctgctgcagc catctgagct acaggcatag
     2641 ccgccgaaac tccgaatttc tcttccagag cttttaccaa ttcagccagc tccagaacag
     2701 tcattttttc aatagattca ataatttctt ctttagtcat gattaatcct ccttctgagc
     2761 attctttttc tcttcaatag cttgcaaaac tcccactaag cttcgcaata atccttgcaa
     2821 aactcccacc aaaccagtaa taggggcttt cattcctcct actacctgag caattaattg
     2881 ctcccgagaa ggaagttgag ccagacgctt aatttcttcg ggctttaaag ggttacgatt
     2941 cagccaaccc cctttgactt ccagctgagg cagtttttta ccagcatcgg ccaataactt
     3001 cgctatttgt accgcatcca gataagaaaa agcaaaggcg ttttgccctc tcatgagttc
     3061 atcatcatag ggaatgcccg ccttatcgag agcaatgcga gccagagtat tcttaactac
     3121 ttttatctct ccctgggcat tcttcaagct tcgacgtaac tcgttcatac tctccacttt
     3181 aattccctgg taattaacta caaacactgc ctcgctcttt tgtagtttct catatacttc
     3241 ctgccagatt ttttctttat ctactttttg caagaattac ctacctcctt cccataaaaa
     3301 aagacccttc cgggggaaga gtcttttttg gtcaatttcc aaccttcact tctctcgcga
     3361 gccttaccat gctcgccaga gaaaagattc ctactcttgc ctccccagga tatttaagct
     3421 aaatgccccc ggttctcagg cctgtattta aatgtcttat tatattatgc taccctcttc
     3481 tctaaactct ctaacgctaa tttggggtct accgggatac ctggccccat agtcgaggaa
     3541 ataactatct tcttgatata gcgccctcga gaagcaggcg gctttaaccg taccacggca
     3601 tccagcaaag cgcaaaaatt atccagcaaa tgttccaccg gaaaagatgc ctttcctata
     3661 ggagcatgga cgttaccata acgatcattg cgaatctcta ttcgtcccgc ctttatttct
     3721 tttaccgctt cagctacatc aaaggttacc gttcccgttt tggcgttagg catcagaccc
     3781 cgcggcccta aaactttacc cagctttccc actaccctca tcatgtccgg agtggccact
     3841 gcggcatcaa attctaacca gccgctctga attttatctg ccagctcttc tccccctaca
     3901 taatcagctc ctgcttcctg tgcttcccga gctttttccc cttgagcgaa aactaaaacg
     3961 cgtacttttt tgcccacccc atgaggaagg gaaaccgcgc ctctaacctg ctggtcagat
     4021 tgcttagggt caattcccag atttatagcc acttctattg tttcatcaaa acgggcatta
     4081 gcagtctctt tcacgaggcg aatagccgtc tccgggtcat acaacttacc tgcttctata
     4141 ttattttttg ctgtcaaata tctcttacct ctatctgcca ttatccattc ctcctcatta
     4201 cccttctatg acttctactc ccatacttct ggctgtcccc tctatgatct taactgctcc
     4261 ttctaagtca ctggcgttaa gatcgggcat ctttttctcg gcaatttccc gaatcttaga
     4321 gcgaggaatt ttacccactt ttacccgatt aggttctccc gaaccttttt ccacccccac
     4381 agcctgctta atcaaaaaag aagcaggggg ggttttgcat ataaaagtaa aagagcgatc
     4441 tccgtatata gtaatctcta ccggaattaa tacccctcgg tctttttccg tagcagcgtt
     4501 gaaagcctta cagaactcca ttatgttaac tccatgctga cctaacgccg gacccactgg
     4561 aggagcaggg gtagccattc ctcccgggat ttgcaatttt actactgcta ttattttttt
     4621 tgccattctt tctctgccac ctctttacag tttctcaaca tcagaaaaat ccagttctac
     4681 tggcgtctct cgaccaaaaa tggaaagaag tacggtcatc ttgccttttt cctggtctac
     4741 gctttccaca gtcccagtat agttcaaaaa gggaccggca attactttca ccatctctcc
     4801 cttttctaca tccagccggg gaactccttt ttctaccccg gtctgacgca aaattacgct
     4861 aacctcttcc tcatttaaag gttcaggctt catacctgac cctacaaaac cagtgactcc
     4921 cggagtattg cgcaccacat accaggaacg atcgttcatt atcatctcca ccataacata
     4981 ccccgggaaa acctttttct tggtaaacct cttctttccc ctcttcacct ctattgcttc
     5041 ttccatggga acgactaccc gaaaaatctg gtcctgcatg cccatagaag taattctccg
     5101 ttccagatta gcttttactt tgtgttcgct acccgctaag gtatgaacca cataccactg
     5161 ttttgacatt tctatttcat tcactactac atcaccttag ataaagattc actaaaaaag
     5221 tgagcaataa atctactgcc cctaaaaacg ctcccgccag cacaataact accaacacag
     5281 tcaaagtatt attccagagc tctttcttgc taggccaggt tactttacgt aattcattcc
     5341 aagcatcacg aaaatactgt ttcagcgaac gaaaccattt gcccaaccgt ttcacccatc
     5401 cttctacatt acacttttag gaaaaatcaa cttcctctag cccaaagcca ctgaggaatt
     5461 ataagttttt tcttcttatc tgtcaattaa actaccaatg tttatctttt aaggttctga
     5521 agttttcttc tctgttttat ctgctacccg ctctatttct gcatctatga aagaacgcat
     5581 agctttcagt acttctactc ttgcctgaga aaggtgttga cgagcttcct cattgagaaa
     5641 aggacctaac actttttcca tgggcttaaa aaatacctct tcaaacacct ctcgggctct
     5701 ttccttttcc tccatagtct tcacctccct atcacttcag gtgtcttttg accaggtctt
     5761 ctgcttcttt ctcgcctttt agtgcctcaa aatccaccac aaaagctccc tgtgattcca
     5821 ttttagacat taatttcttt aaagccttat ctgttccaaa caggttagga cgaacataag
     5881 ctaccgccct tttaccttcc attccacttg ctccttgcaa aaagtttact acctcctgag
     5941 atattttgcc tccccacaag ctcaaaactt gagaaccaac aaatatcaaa tcaaaagggc
     6001 gcaaactaac cggagaggtg gtatcttctg cttcaataac ttttaccgtc caaccctctt
     6061 tttcaaagat accaaccata taacgggcca gattggctac acgtttatct ttgctactgt
     6121 gaataaaagc cactcgcaaa aatccattca cctcctttca tattataata cattttccga
     6181 tttcttataa gcaactgtaa cccaggagga atctaccttg atttcttttt ctttcaaact
     6241 cagcatttta gctttttctt gaagaagaga aaaatctttc tcttctcctc ctgagagaac
     6301 cagaatgcct tcttcgttaa gcaagttgtt catagtttcc aaatggagaa gatgaaaatt
     6361 agtcccgata ttacacacta taaaatcaaa gttttctttt atttcttcta tttcctcctc
     6421 tttaaagttt actcgagaaa aatctatatc gtttaaagat aaattacgcc tcagctcttc
     6481 tccggcatgg gggtcgatat ctaaagctac aattcttccc ccctcaagcc ttgccgctaa
     6541 aatggaaaga atacccgagc cacttcccac atccagaaaa ctctgtcctt tttgaaagta
     6601 tttatctatc atatataaac accagtaagt ggtgggatgt tgaccggtgc cgaaagcata
     6661 agcagggtaa atcacaagct ccacagccgc gccttgcggg tcttcccagg ggggcctcac
     6721 cactattctt tctcccaccc ggatgggctt aaaactctct cgccatttat aagcccattc
     6781 cccttctccc tcctcttctt ctctaccttt cactcccggg ggaaggacaa tggagttttc
     6841 cgagtaaact atcatctcat caaaccctga ctcccatacc cctaatatat tttgttcttg
     6901 taactgaagc aggagttttt ccttttcttc ttcttttata gttattatag ttttaaacca
     6961 tttcttcatt gctatttctt ctttctccta ccactaaaag tcctgaagct acttttgctc
     7021 ccactcccaa cacttcaaac agagcatcat actctcgaga gaattctaaa aactccttat
     7081 acagagaggg attattcact attttcttta aagtacgagg aggcaggagt cgagccagag
     7141 ttcccgaagc caccacccgc aaaggtctaa acccctcctc tattaaatca tctaaaattt
     7201 cagaaggggt aaaagcatgc agagggggaa aaatatagcg ataaaggaga ggaattttct
     7261 cctctaagtt ttcccagaaa gaaggatgat cccagttacc ttctctaaaa aaacgacgag
     7321 aaatctccaa gctatttctg aatcgcaatt ccgtctccac tgctactgga acctgtcccc
     7381 accgatttac taccgaaacg attagcttat ctttagttac tctccccagc tcctcgagag
     7441 cctttttttg ctccggataa gcataggaaa caggggcatc cagagaaata gtaagatcaa
     7501 aataacggtc agaaaaagag gacagatctt gcaccttccc tacctgaaac tcgatggaag
     7561 aaacacccct gtcccctgct aattttttag cttcttctat catccgggga gaaaggtcaa
     7621 gatgagtaac ctggcatcct ctttctgcta aaggaataga atagcgtcca tagcctcctc
     7681 cggcatccag aaccttcaat cctggttgta aaaaaggctc tatttctaac catacgagat
     7741 ccgtgtgaat ccttttttcc cagtttttat cccgcttttt ttctcttttt acccctccat
     7801 tccaaaaaag gcgatgagaa gtttctccct tcactaatca accacctttt cttctatctc
     7861 ccgctttaga ttcttcaata attcatccag ggtgaaagag cctatatccc cttttttacg
     7921 tttccggata ctcagtgtcc ggttctccat ctcccggtct ccaatgatag caaggtaagg
     7981 aactttttcc acttctgctt ttctgatttt agcccccaga gtggcatttt cgtcattaat
     8041 ctccactcgt atttcttcgc taaaaataac gtcttttatt tcccgggcat aatcgatatg
     8101 tctttcggcg ataggcaaaa ttaccacctg cactggtgct aaccacaggg ggaaagctcc
     8161 ggcaaattgc tcaattaaaa tacctaagaa tctttcgata cttcctaaaa ctgttcggtg
     8221 gagcatcacc ggtcggtggc ggtttccatc ttctccaatg tagtacaggt caaatttttc
     8281 aggcatagca aaatccagct gaatagttcc acactgccat cttcttccca gacaatcacg
     8341 gagatgaaaa tcaatctttg gcccgtaaaa agctccttct cccgggttaa ctcgatagtc
     8401 aacttctaac tctcttagcg cttcttctaa cgaccgggta gccagatccc acatctcatc
     8461 agaccccatg gagttctcag gacgagtgct caactctact tcataagtaa aatcaaacag
     8521 gcggtaaaaa taatcagcta actcaattac ccctataatt tcctgtttta cctggtgagg
     8581 ctccataaag atatgggcat cgtcttgagt gaaacaccgt actctcatta acccgtgcaa
     8641 aactccggaa agctcatgac gatgaaccaa ccctaactct gcccaccgta agggtagctc
     8701 tcgatagcta tgcagctggc ttttaaaaat taaaatcccc ccgggacaat tcatgggttt
     8761 tatagcaaaa tcccggtcat cgattttagt aaaatacata ttatcccggt agtgttccca
     8821 atggccggat tgttcccaga gagcccgttc taaaatcata ggagttttta cttcctgata
     8881 accccgcttt aagtgttccc tacgccataa gtctaaaaga gcattgataa ccaccatccc
     8941 ccgagggtga aagaagggaa aaccaggacc ttcttcgtgc agactgaata aatccagctc
     9001 tcttcccaag cgacgatggt ctcttctttt agcttcttct ctccgcttca agaactcttg
     9061 caatttctcc tccgaatcaa aagaaattcc atatatccgc tgaagcatgg ggttatcctc
     9121 tcttccccgc cagtaggctc ccgaaacgct taaaagctta aaagctttta tgtagccagt
     9181 agaaggaaca tgaggccccc ggcagaggtc taagaattct ccctgttcat aaagggttac
     9241 tttgctctct tctatctctt ctaaaatttc tactttataa gtttctcctc tttcggcaaa
     9301 tattttttgc gcttcctcct tagaaacttc ttttcgccga aaaggaaggt cttcctggat
     9361 aatttttctc atttcttttt ctatctcttc taagtcttcg tcggtcaaag gacgaggaat
     9421 atcaaaatcg tagtaaaaac cttcctcaat agctggacca atggccagct tggcttcggg
     9481 aaacaatctc tttaccgcct gagccatgat atgggaagta ctatgccagt aaatttctct
     9541 acctgcttca tcggcaaaag agaggtaatt ttttactttc tcctcttccc ctatatccac
     9601 caaacgacca tcttcggtaa ccgcagcgat ggcttcttta gatttcacgt tcataaattt
     9661 tcctccttat atatagtagc tctcctactt ccgatatcct gcattttatc acttcattta
     9721 aaccactttt gagcacaaat tattatattt aatatattac cacatcgctg aaaaatccgg
     9781 gatatatatc ctgtggataa taaaattaag gcatcaatga gagctcatcc cctcatttcc
     9841 agtagcagct ttccgagaaa gacatttacc aggtgataca actatcatct tgcgtagccc
     9901 tcgaaaaatt cccggcagtc aaacgggtac ggaaatagat cgctgagctt cgtaaccacc
     9961 tctccgaaag tagcacataa atatatacac cacttgcaaa gaacataagg gctcgccgga
    10021 acagcaacca ataataatag gttttgaaaa aacggatggt ttttaaatgg acattttcca
    10081 ggcacggctt tttaaacaga cgttctttaa cagacagttt ttaaacgcaa caagcttgtt
    10141 gttagccaaa ccgcaaaaga ttcgaaaaca ctcttgcatt tctaaattga cggatgttat
    10201 gaatagtagg tataatcaat gttagcttga cgcctcaggc gctgaatgaa gaaaaggaca
    10261 agagaaaatt atggaaaaga aaatagcaat aatcggctgc ggaagcatgg gaaaaatgct
    10321 tctggaaaaa ataatcaatt ctaaaataat accagaagac aatttattcg tagcaaaccg
    10381 tacacttgaa aaaatacaag ttttaaaaga aaagtataag aatatgcata tttgcagaac
    10441 aaacaaagaa gcagcagaaa attcagacat tatcttcatc tgcgtaagac catcagatat
    10501 aaagaccgtt ctgctggaaa taagagatgt gcttgataat agcaaacata tcatttcatt
    10561 aaacggaagt attttattcg aacaaataga aacagtatgt aaaaataata aaatatcgaa
    10621 ggtaattcca agcgttaccg cagaagtaaa tgaatcacaa acattagtat gccataatag
    10681 taaagtaacc aaagaagatg aaatagtatt aaataaaata ttgggaacat ttggaaaagt
    10741 aatagagctt ccagaaaatg aaatgggaat gggagcagag ttggtaagct gtatgccagg
    10801 attcattgca gcaatattca atgaaattgt cgttgtagcg caagaacata ccaaaatacc
    10861 agaagaacag ataacaaaca tgctactaaa tacaatgatg ggaacgagca agttaatgct
    10921 ggaaaacaag ttatcatttg aagaagttat tatcagggtt gctactaaag gcggaattac
    10981 ggaagaaggt acaaaggtaa tacatgagaa attaccagga atagtaaaag aaatgtttga
    11041 gaaaacatta gagaaaagaa aattaacaac agaaaatgca ataaaagcat ttaatagtta
    11101 agcatacaaa agctaatacc tgcatcaagc ttacttacag ccaaccgtga aggtaaaagc
    11161 catccctctc ctaatgaaag ccttaattaa gtagcccacc acttaaacca atgatagacg
    11221 ctcatgctgc tttgcagcac cagatgacaa tgaaaacatg aggtctattc ttgcagcttt
    11281 ttgttttttt taaatctttg ccttttattt ttagtttttg tctttcgggg attatagggg
    11341 gctctgctcc cttattgtct gttctgtcat tcccgaaatc ttcaatcggg aatccagata
    11401 tttttatcct gaaacaattt agattcctga tagagacatt caggaatgac ggacgcggag
    11461 gttctttctc cgggttattt ttgatctttt tcttttgtct ttatgtttct tgctttttcg
    11521 ggggttataa ggggggcttc gcccccttat tgccgtttac agtatagatg gatacttaat
    11581 acgaaaaaga accatggact attgatagaa ggggagggaa attttttaaa cttttaagga
    11641 aggctttttt tcttttatct acttctggtg ggcgacactg gactcgaacc agtggcctct
    11701 tccgcgtcaa ggaagcattc tccctctgaa ctagtcgccc actaaccatt ttctggaggc
    11761 ggcactcgga ctcgaaccga ggatgaagga tttgcagtcc tctgccttag ccacttggct
    11821 atgccgccat taactggagc ggaagacggg atttgaaccc gcgaccccca ccttggcaag
    11881 gtgatgctct accacctgag ctacttccgc tcatcgtggt gccgagaccc agaatcgaac
    11941 tggggacgca aggattttca gtccttcgct ctaccgactg agctatctcg gcgtacaaat
    12001 caactctggc ggggtcgacg ggatttgaac ccgcgatctt caacgtgaca ggctgacgtg
    12061 ttaggccagg ctacaccacg accccgcaca tctccatttt aatagttaaa accatttttg
    12121 gcaagaggt
//
LOCUS       DAOPSS010000042        12024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4484_length_12024_cov_9.653271, whole genome shotgun sequence.
ACCESSION   DAOPSS010000042 DAOPSS010000000
VERSION     DAOPSS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4484_length_12024_cov_9.653271"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..798)
                     /locus_tag="PKN31_03910"
     CDS             complement(310..798)
                     /locus_tag="PKN31_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOA99102.1"
                     /translation="MARRLLPVRREERRVSPARQLWDLFDLRTDLDEIFDELMEFPFF
                     STPSLVAGFPPVDIAETENEVIAKVEVPGVDPKDLEVHVTEDTLNIKGEVKDEWEEKK
                     VGYYARERYYGTFERTLPLPAKVKQDEVKAQYKDGVLHITLPKVEPSTPKTKKVEIEV
                     GK"
     gene            complement(1057..1950)
                     /gene="ftcD"
                     /locus_tag="PKN31_03915"
     CDS             complement(1057..1950)
                     /gene="ftcD"
                     /locus_tag="PKN31_03915"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOA99103.1"
                     /translation="MKRVIQSAINLSTSSPFLVEKIVEEISGLPGLKVADYSSDPDHN
                     RSVVTLIGEEESLEKAVLKIVEIAEKEIDISRHRGEHPRVGAVDVIPFTPWRGVDMED
                     CKKLAWKVGEKVAERFDVPVYFYGEAALREEHRDLSRIRRGGYELLKEEIDNVLERHP
                     DLGPAKIHPTLGAVVIGARGPLVAFNVNLKTQDLDIAKAIARKLRGETGGLRYVKAIG
                     VSLKSKDMVQVSMNLLDFKKSTIYQAFELVKLEAKRWGVEVKGGEIIGLLPLEALVDV
                     AGFYLGIPELSVEQVLEYHLF"
     gene            complement(1951..2466)
                     /locus_tag="PKN31_03920"
     CDS             complement(1951..2466)
                     /locus_tag="PKN31_03920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOA99104.1"
                     /translation="MKALLVIDLLNDFVHPRGALFVGEKAKEVITFSQELVSECRKEN
                     IPIIYVCDSHRPDDQEFTLFPPHAIEGSWGGKVTEELTPQAGDYIIPKRRFSAFFGTS
                     LDLLLREKKIEELEIVGVCTNICVLYTSAGARMLNYQVVVREKGVTSFDLAAHEWALR
                     EMKNTLGVEVK"
     gene            complement(2463..3128)
                     /locus_tag="PKN31_03925"
     CDS             complement(2463..3128)
                     /locus_tag="PKN31_03925"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOA99105.1"
                     /translation="MMDFDSKETIEQRKNMVERQLILRGIKNQRVIEAFLRVPRHMFV
                     PPSHRDLSYQDTPLPIGYGQTISQPYIVALMVELLNPQEGDEVLEIGTGSGYQTAILA
                     ELVKMVYSVERIPELIKRAGGILSQLGYRNVVLVEGDGSKGLPRFSPYDKIIVSACSK
                     KIYPAWVEELKEEGIIVLPLEAERGQNLVRGIKRKGKLVLEDHGGCVFVPLVEDELQG
                     ESK"
     gene            complement(3128..3409)
                     /locus_tag="PKN31_03930"
     CDS             complement(3128..3409)
                     /locus_tag="PKN31_03930"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOA99106.1"
                     /translation="MAQWVRGRLVLRGLVQGVGYRYFVLRKAGEFDVTGLVRNLPTGE
                     VEVIAEGKKEEVEKFFEEIKQGPVSAHITSYSEEWFPFSGLYSDFRVGY"
     gene            complement(3427..4221)
                     /gene="surE"
                     /locus_tag="PKN31_03935"
     CDS             complement(3427..4221)
                     /gene="surE"
                     /locus_tag="PKN31_03935"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOA99107.1"
                     /translation="MKRILITNDDGFKSPGIEALVRAFKGLGEVILVAPQDEKSCSSH
                     SITSRHPLRVREVEVGGVRGYAVEGTPADTVILALRLFDDGLIDFVISGINRGPNLGF
                     DVFYSGTVAAAMEAAMSGIPSLAVSLVVNNYFNYQLAAEVAVEVFETFEDLLRREKNA
                     VLNLNIPDVPDKKDLQGWSITTLGDRFYLTRVKEIESQNPEVREFVLEEEKREASFPE
                     NSDFQAVWCSRVSITPLRPNLTDFILGEELREILQNKGLSSLYHPG"
     gene            complement(4218..5609)
                     /locus_tag="PKN31_03940"
     CDS             complement(4218..5609)
                     /locus_tag="PKN31_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOA99108.1"
                     /translation="MRENYDVIVVGAGPAGIFTALELSERSDLQILMLDRGKNIGRRS
                     CPARENNSICQKCSPCSLLCGWGGAGAFSDGKLTLSQEVGGQLTSYIDGQLFREVVQY
                     VDSTYLRFGGTDKIYGTDSEKVEELRRKAEIAELVLVPAKIRHLGTDRCWDILKNMYD
                     YLSDKVEIKTSLPVEKILVENGKVEGVKLRGGEEVKSKFVVVAPGRIGADWLRQECLR
                     LKLPVENNPVDLGVRVEVPAAIMDEFTDALYELKLIYYTPTFDDQVRTFCMCPRGEVV
                     TEYNDGIITVNGHSYGNRESSNTNFAILVSTNFTEPFREPIAYGRYVARLANMLSGGV
                     IVQRLKDLQLGRRSTPERISKSPVKPTLQGASPGDLSFVLPFRFLQDVQEMLLAVDKF
                     IPGINSPHNLLYGVEVKFYSSRVKLNSYLESDVENLFLVGDGAGITRGLVQASASGVV
                     VAREIVRREGGKV"
     gene            complement(5610..6539)
                     /locus_tag="PKN31_03945"
     CDS             complement(5610..6539)
                     /locus_tag="PKN31_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA99109.1"
                     /translation="MRVGVIGVGYLGQHHARVYSELPGVELVGVVDINKERAKEVAER
                     YFTSAFFDYQDLFGKVDAVSIVVPTVIHRDIAGRFIEQGINILIEKPVTTSLEEAREL
                     MEMANKKNVVLQVGHIERFNSAVVELSKIVDHPIFIDCSRMGPYVNRNTDVGVVLDLM
                     IHDIDIVMSIVRDKVVKINASGYPVFSREEDIANAQLVFSNGCIANLTASRITRKKIR
                     RMEVTQLDSFISIDYLEQELAVYKKTSSSFPYLLMEKPPMQKGEPLRLELEHFIKCVR
                     NGEKPLVGLEEGTNALEVALNILEEIKKKHGDS"
     gene            complement(6574..7674)
                     /locus_tag="PKN31_03950"
     CDS             complement(6574..7674)
                     /locus_tag="PKN31_03950"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOA99110.1"
                     /translation="MVNIPLSKPCLGEEEKRAVNEVLDSGFLAQGENVRKFEEEFARY
                     VGVKEAVATSNGTTALFVALKALGVKEGDRVITTPFTFVATASSILQCGGVPVFCDVD
                     EKTFNLDPGKLEDILKKERNIKGIIAVHLYGLPCDLEEIAFLTQKYNLFLLEDCAQAH
                     GAECQGRKVGSWGDMGAFSFYPTKNMTTGEGGMITTNDSLLAQECRMLINHGSRKRYY
                     HEALGYNFRLTDIGGALGRVQLSKLEQFNEKRRRNAAFYNQELADLPFIDVPYLPPYA
                     LPVFHQYTLRVKGARDALLQFLRDRGIGCEIYYPLPLHHQPFLQNLYSASSFPVAENL
                     AREVLSLPVHPQLTREDLNTIILGVREFFLNH"
     gene            complement(7674..8864)
                     /gene="aroC"
                     /locus_tag="PKN31_03955"
     CDS             complement(7674..8864)
                     /gene="aroC"
                     /locus_tag="PKN31_03955"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOA99111.1"
                     /translation="MLEFHTAGESHGPAIITLIRGLPANLEVDIPFINRELSRRQSGF
                     GSGGRMKIEKDEVEILGGVRWGKTLGGPVVFEVKNRDYENWSTLMDPRGSAPSSYEEI
                     TIPRPGHADLGGMVKYRFSDLRNVIERASARETVGKCVAGAVAKIFLRHFGVKVGGFV
                     ESIGEISAQEEMSWEEKISRAESSPLHTYDPEREKEMIELIEKVGEEGDTLGGTFVVV
                     AQGVVPGLGSYGEIQERLDSQLAFFLMGIPGIKGVEVGEGFRSATLRGSQFHDEIFYD
                     SRRLPFPFYRQTFRSGGVEGGISVGDIMWVRCAMKPIPTLRRGLMSVDVREKKAVSAR
                     FERSDICAVPRAMVVGEAMLAWVLAAAYRKKFGGDSMEEIEAYFKDYLDYLESFHSLE
                     DGEK"
     gene            complement(8839..9156)
                     /locus_tag="PKN31_03960"
     CDS             complement(8839..9156)
                     /locus_tag="PKN31_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOA99112.1"
                     /translation="MTSHLPHILSNCFLSAVLSHREDLPQYAGPSFGDWVRIGGSSPR
                     MWRDIFITNRDKILKTIGLFQRELDKMIEIIKESEEEELLSLLQEIAHLKEESIAGVS
                     HRR"
     gene            complement(9186..9701)
                     /locus_tag="PKN31_03965"
     CDS             complement(9186..9701)
                     /locus_tag="PKN31_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOA99113.1"
                     /translation="MEELAIAIIGLGLMGGSLGLELSSWPRTKFIKGYDIDRKAAEKA
                     KELGAVNFISPSLEEAAQGVDLVFLSLPVMSVPPVFLELRPHLSPSTRVLDLGSTREW
                     VWGEISPALQGLEYSGFHPMGGGERSGIEHARKGILRNVPILVSPPPASESGKNMVEE
                     IARFLEGQVFF"
     gene            complement(9703..10719)
                     /gene="aroF"
                     /locus_tag="PKN31_03970"
     CDS             complement(9703..10719)
                     /gene="aroF"
                     /locus_tag="PKN31_03970"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOA99114.1"
                     /translation="MIIVLRSGATQEQIDDVVKRLEKLGFGAHVSQGVERTIIGAIGD
                     ERRVNLEEKIGVLPFVEKVIPVLAPYRLTSREFREADTTVKVGDVEIGMGKFVLIAGP
                     CSVESEEQIVETALRIKEAGAKILRGGAFKPRTSPYSFQGLAEEGLKFLAKAREVSGL
                     PIVTEALGVEELPLVAEYADMIQIGARNMQNFRLLEAAGRLHKPVLLKRGMMNTVEEL
                     LMSAEYILAQGNHQVVLCERGIRTFEPSTRNTLDLSCIPLVKMQSHLPILADPSHGTG
                     RSDLVETMSLAALAAGADGVIVEVHPNPVEALSDGPQSLTPEQFYHLVREIKKLAVVM
                     EKEV"
     gene            complement(10732..11757)
                     /gene="rlmN"
                     /locus_tag="PKN31_03975"
     CDS             complement(10732..11757)
                     /gene="rlmN"
                     /locus_tag="PKN31_03975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOA99115.1"
                     /translation="MRKKNLIGLLPSDIYSWLEEEGEPSFRARQIFQWIYEKKADSFF
                     DMTNLPVALREKLDSAFSLGSSQIKEIQKSKDGTVKFLIHFPPQDNVEAVLIPHRDRR
                     TLCLSTQVGCPAGCTFCATGLAGFRRNLTYQEIVEEIWVIEKETGMTANNLVFMGMGE
                     PLLNYENVHRALQVINSPEGFRKGARRMTVSTVGIPDKIVKLGRDWPEVNLAWSMHAP
                     RDELRNLLIPLNKVYPLEQVLRAIKEYLAVTHRRVTIEYTLWNEINDSKEDAQEVAKL
                     LKKLLIHVNLIPGNLVPSSAFSPPSPRRVKAFASVLEENGIQVTIRKPRGQDIQAACG
                     ELYRIYG"
BASE COUNT         3341 a         3163 c         2173 g         3347 t
ORIGIN      
        1 tttcgggaat gacggatgag gcatcacccc tgaatggttc tacgaggggt ccaaataaat
       61 aggacgggaa accctctatg tattttccct tttccctcaa ctctttttct ttccttcatc
      121 ctttgagggt gaaggaaagt caattataag ggggtgccgc ccttttataa cctccaaaaa
      181 ataaagaagt ataaaagatg caaggataat cgaaaaaact actttgcaag aggggaaagt
      241 tggctgtggg tttgtgtctg accaataaaa aaagcccgga gcaaactccg ggcttttttt
      301 atttacctac tatttaccta cttctatttc tacttttttc gttttgggtg tactgggttc
      361 aactttggga agagtaatgt gcaaaacacc atctttgtat tgagccttca cctcatcttg
      421 ttttacctta gcaggtaaag gcaaagtgcg ttcaaaggtt ccataataac gctcacgagc
      481 ataatagcct acttttttct cttcccactc gtccttcact tcacctttga tgtttaaggt
      541 gtcctcagta acatgcactt ctaagtcctt gggatccact cctggtactt ctactttggc
      601 tatcacttca ttttcagttt ctgctatgtc tacaggtgga aacccggcaa ccagagaagg
      661 tgtactgaag aaaggaaact ccatcagctc atcaaaaatc tcatccagat cagttcgtaa
      721 gtcaaagaga tcccacagtt gtcttgctgg acttactctc ctttcttctc tccgaaccgg
      781 taacaatctt ctggccattc catttccctc cttttatcta tttatttatt ttccgactcc
      841 tattataatc agagaaagtc agtttgtcaa taggttggtc agagaaagtc aaaaaaattt
      901 ttcagaacgg tccataacag aaacagagaa aaagagaggc gctccattgt ttatagcatt
      961 ctccaaacct gctacagtta tcttttccga tgagaagaaa aaaagatgga gtgtccagag
     1021 agggatactg cagtgaggtt tcacagctaa ctgtaattaa aagagatgat attctaaaac
     1081 ttgttctacc gagagctcgg gaatgcccag ataaaagcca gccacatcta ccagagcctc
     1141 gagaggcaaa agcccgatta tctctcctcc ttttacctct actccccaac gcttagcttc
     1201 cagttttacc agttcaaagg cctgataaat ggtgcttttc ttaaaatcta aaaggttcat
     1261 agaaacctga accatgtctt tgctttttaa gctaaccccg atagctttaa cgtagcgtaa
     1321 tccccccgtc tcaccccgca gcttgcgagc gattgctttt gcaatatcga ggtcttgggt
     1381 tttcaggttt acattgaaag ccacgagagg cccccgggct ccaatgacca ccgctcctaa
     1441 agtggggtga attttagccg gacccagatc cggatgtctc tcgaggacgt tatctatctc
     1501 ttccttcagg agctcatatc ctccccggcg gatgcgggaa aggtcccggt gttcctcccg
     1561 gagagccgcc tccccataaa aataaaccgg gacatcaaat ctctcggcta ctttttctcc
     1621 caccttccag gctaatttct tacaatcctc catatccact ccccgccagg gagtaaaagg
     1681 aatcacatct accgctccca ctcggggatg ctctccccga tgcctggata tatctatttc
     1741 tttttctgct atctccacaa ttttaagcac ggctttttct aagctttctt cttctcctat
     1801 gagggttacc accgagcgat tatggtcggg gtcggaagaa taatcagcca cttttaaccc
     1861 aggaagacca gaaatttctt caactatttt ttctacgagg aaaggagagg aagtgctcaa
     1921 attaatagcg ctctgaataa ctcttttcaa ttactttacc tctaccccta aagtgttttt
     1981 catctcccgg agggcccatt catgagccgc caaatcaaaa gaggtaactc ccttttcgcg
     2041 aactaccacc tggtagttaa gcattcgagc ccctgctgaa gtgtatagaa cacaaatatt
     2101 ggtgcatact cccactattt ccagttcttc tatttttttc tcccgcaaaa gaagatctaa
     2161 agatgtaccg aagaaggcgc tgaaccttct tttggggata atatagtcac cggcttgagg
     2221 ggtgagctct tctgtgactt tccctcccca gcttccctct atggcatggg gcggaaagag
     2281 agtaaattcc tggtcatcag gacggtgaga atcacaaaca taaatgatag gtatattttc
     2341 cttccgacat tcagaaacta attcttgaga aaaagttatc acttctttag ctttttctcc
     2401 cacaaaaagc gctccccggg gatggacaaa gtcgttcaaa aggtctatca caagcagagc
     2461 tttcattttg attccccctg tagctcatct tccaccaaag gtacaaaaac acagcctccg
     2521 tggtcttcta acaccagttt tccctttctc tttatccctc gcaccagatt ttgccctctt
     2581 tctgcttcca aaggtaaaac aattatccct tcctctttca gctcttccac ccaagcagga
     2641 taaatctttt tagaacaggc acttactatt attttatcgt agggagaaaa ccgaggtagc
     2701 cccttgcttc catccccttc tacgaggact acattacgat atcccagctg agagagaatg
     2761 cctcccgccc gctttatgag ctcgggaatc ctctccacgc tatagaccat tttgactaac
     2821 tcagcgagaa tagccgtttg gtatcctgag ccggtgccta tctctagaac ctcgtctcct
     2881 tcctgggggt ttaaaagctc taccatcaaa gccacgatgt aaggttgaga aatggtttgc
     2941 ccatatccaa tgggaagagg agtatcctgg taagataggt ctctgtgaga aggaggaaca
     3001 aacatgtggc gaggaacacg caaaaaagct tcaatcaccc gctggttctt tattcctcgc
     3061 aggattaatt gccgctccac catatttttt ctctgctcta tagtttcctt gctatcaaaa
     3121 tccatcatca gtagcccacc cgaaaatcag aatacaaacc agaaaaagga aaccactctt
     3181 ccgaataaga agtgatatga gcacttaccg gtccttgttt tatttcttcg aagaattttt
     3241 ccacctcttc cttttttccc tctgctataa cctccacctc tccagtggga agatttctca
     3301 ccaatcctgt cacatcaaat tctcctgctt tacgcaacac aaaataacga tagcccactc
     3361 cttgaaccag gcctcgcaaa accaatcttc ctcgcaccca ctgagccaca atctgccccc
     3421 ttaactttac ccggggtgat agagagaaga cagaccttta ttctgtaaga tttcccgcag
     3481 ctcttctccc aggataaaat cagtgagatt agggcgcagg ggagttatag aaacccggga
     3541 acaccagacc gcttgaaaat cagaattttc tggaaaactt gcctcccttt tttcctcctc
     3601 caaaacaaat tctctcacct ccgggttctg gctttctatc tccttcaccc gggttaagta
     3661 aaagcggtct cccagggtgg taatagacca gccctgcagg tcctttttat caggcacatc
     3721 aggaatattt aaattcagca ccgcattttt ctctcttctt agaaggtctt caaaagtctc
     3781 aaacacttcc actgccacct cagcagccag ctgataatta aaatagttat ttaccaccag
     3841 ggaaactgca agggagggaa ttcctgacat ggctgcctcc atagctgcgg ccactgttcc
     3901 agaataaaaa acatcgaaac ctaaatttgg accacgattt atccctgaaa ttacaaaatc
     3961 aatgagtccg tcatcaaaaa gacggagagc cagaattacc gtgtctgccg gagttccctc
     4021 caccgcatat cccctcactc ctcccacttc cacttctctc actcgcagtg gatggcgaga
     4081 ggtaatgctg tggctggaac agctcttctc atcttgaggg gcaaccagta tcacttctcc
     4141 taaccctttg aaagctctaa ccaaagcctc aatacccggg cttttgaatc catcatcgtt
     4201 agttattaaa atcctcttca tacctttcct ccttctctcc ttactatctc ccgggccacc
     4261 actactcctg aggctgaagc ttgaaccagg cctcgagtaa tccccgctcc atctcccacg
     4321 agaaaaaggt tttccacatc gctctccagg taagagttca atttcactcg agaagaataa
     4381 aatttgactt ccactccgta taaaagatta tgaggagaat taatgccagg aataaattta
     4441 tccactgcca gtagcatttc ctgaacatcc tgaagaaagc gaaaaggcaa aacaaagctc
     4501 aaatctcccg gactcgctcc ctgaagagtg ggtttcaccg gtgatttgct gattcgctcc
     4561 ggcgtagagc gcctccccag ttgcaaatcc ttcaacctct ggacaatcac tcctccactc
     4621 aacatgttag ccaacctagc tacatacctt ccataagcaa tgggctcccg aaaaggctca
     4681 gtaaaattag tgctcaccaa aatggcaaaa ttggtgttgg agctttcccg attcccataa
     4741 ctgtggccat taaccgtaat aatgccatca ttgtattctg taaccacttc tccccgggga
     4801 cacatacaaa aagtgcgcac ctgatcatca aaagtgggag tataataaat cagtttcaat
     4861 tcatagagag catcggtaaa ctcgtccatt atagcagcgg ggacttccac tctcactccc
     4921 aaatccaccg gattattttc tactggtaat ttcaagcgca aacattcctg acgcagccaa
     4981 tctgcgccta tcctccccgg agctaccacc acaaatttgc tttttacctc ttctccaccg
     5041 cgaagcttaa ccccttccac tttcccattt tcaactaaaa ttttctccac cggaagagag
     5101 gtttttatct ctactttatc actcaggtaa tcatacatat tttttaaaat atcccagcat
     5161 cgatcggtgc ctaaatgcct tatcttcgcc gggaccagaa ccaattctgc tatctctgcc
     5221 tttctacgca attcttctac cttttctgaa tctgtcccgt aaattttatc cgttccccca
     5281 aagcgaaggt aggtagaatc tacatattgc accacttctc ggaataactg gccatcaata
     5341 tagctggtaa gctgacctcc cacttcttga gagagggtga gcttcccatc actgaaggct
     5401 cccgctcctc cccaaccaca caaaagagaa caaggagaac acttctgaca gatactgtta
     5461 ttttctcgcg ccgggcaaga gcgccttcca atatttttcc ccctgtccag catgagaatc
     5521 tgtaagtcac ttctttcact caactctaaa gcagtgaaga taccggcagg tcctgcacct
     5581 actacaatta catcataatt ttctctcaac taactatcac cgtgtttctt tttaatttct
     5641 tctaaaatat taagagccac ctctaaagca ttggtgccct cttctaaacc cacgagaggc
     5701 ttttcaccat ttctcacaca tttgataaaa tgttccagtt ccagacgcag gggttcacct
     5761 ttttgcatag ggggcttctc catcaaaaga tagggaaaag aagaagaagt ttttttatac
     5821 actgccagct cctgttccag atagtcgata gaaataaaag agtctaactg agttacttcc
     5881 atccgacgaa tttttttgcg agtaatcctg ctggcggtaa gattggctat gcatccatta
     5941 gaaaaaacca gctgagcatt ggcaatgtct tcttcccggg aaaaaaccgg gtagccagaa
     6001 gcgttgattt taaccacttt atccctcact atgctcataa cgatatctat atcatgaatc
     6061 atcaagtcca gaaccactcc cacatcggta ttgcggttaa cataaggacc cattcgggaa
     6121 caatcgataa aaatagggtg gtctactatt ttggaaagct ccacaactgc cgaattaaaa
     6181 cgctcaatat gtcctacctg gagaaccaca tttttcttgt tggccatctc catcaattct
     6241 cgggcttctt ccagagaagt agtcaccggt ttttctatca aaatgtttat gccttgttca
     6301 ataaatctac ccgcaatatc ccgatgaatg acggtgggaa ctactatgct tactgcatcc
     6361 actttgccaa aaagatcctg ataatcaaaa aaagctgaag tgaagtagcg ctctgctact
     6421 tctttagctc tctctttatt aatatccact actcctacca gttccacacc gggaagttca
     6481 gaataaaccc gggcatgatg ttgacctaaa tagcccactc ctattactcc cactcgcact
     6541 ggatccaaaa ttaccacctc gagtaataat aagctaatga ttcagaaaaa attcccgcac
     6601 tcccagaatt atggtattca aatcttccct ggtcagctga ggatgtaccg gtaaagacaa
     6661 aacctccctc gccaggtttt ctgctacagg aaaggaagag gcagaatata aattctgcaa
     6721 aaagggctgg tgatgaagag gtagaggata atagatttca caccctattc ccctatctcg
     6781 caaaaactga agcaatgcat cccttgctcc ttttactcgc agagtatact ggtgaaaaac
     6841 gggcaaagcg taaggaggaa ggtagggaac atctataaaa gggagatcag ccagctcctg
     6901 gttataaaaa gcggcatttc gacgcctttt ttcattaaac tgctccagct tagaaagttg
     6961 aactcgaccc agtgctcctc ctatatcggt aagacgaaaa ttgtaaccca gtgcttcatg
     7021 atagtatctt tttctgcttc catgatttat aagcatacga cattcttggg ccaaaagaga
     7081 gtcgttggta gtaatcattc ctccctctcc cgtggtcata tttttagtag gataaaaact
     7141 aaaagccccc atatctcccc agcttcctac tttcctcccc tgacactctg ctccatgtgc
     7201 ttgagcgcaa tcctctaaga gaaaaagatt gtatttttga gttaaaaaag ctatttcttc
     7261 caaatcacag ggaagaccat aaaggtggac agctattatt cccttaatat ttctctcttt
     7321 tttaagaata tcctcaagct ttcccgggtc aagattaaag gttttttcat ccacatcaca
     7381 aaaaaccggt actcctccac actggagaat agagctcgcc gtagctacaa aggtaaaagg
     7441 agtagtaata acccggtctc cctccttcac ccctaaagct ttcagagcta caaataaagc
     7501 agtggttcca ttgcttgtgg ctacagcctc ttttactccc acataacgag caaattcttc
     7561 ctcaaacttt ctaacgtttt caccttgagc cagaaatccc gaatctaaaa cttcatttac
     7621 tgctcttttt tcttcttctc ctaaacaggg tttgctcaga ggtatattaa ccattatttt
     7681 tctccatcct caagagaatg aaagctttct aaataatcaa ggtaatcttt aaaataagct
     7741 tctatttcct ccatagaatc gccaccaaat tttttccggt aagcagcagc caaaacccag
     7801 gctaacatcg cctctcccac caccattgcc cggggaacag cacagatgtc ggagcgctca
     7861 aaacgagcag aaacggcctt tttctccctt acatctacgc tcatcaagcc tcgtcgaaga
     7921 gtgggaatgg gcttcatagc acatctaacc cacattatat cacctaccga aatcccccct
     7981 tccactcctc cgctccggaa agtctggcga taaaaaggga aaggcaaacg acgggagtca
     8041 taaaaaatct catcatggaa ttgacttccc cgaagagttg cagagcgaaa gccttcccct
     8101 acttccactc ctttaattcc tggaataccc atcaaaaaga aagccagctg actatctaat
     8161 cgttcttgaa tttcgccata gcttcccaga ccagggacta caccttgagc caccacgaca
     8221 aaagtgcctc ccagagtatc tccctcttcc cctacttttt ctattaattc tatcatttct
     8281 ttttctctct ccgggtcata agtgtgcagg ggagaagatt ctgcccggga tatcttttct
     8341 tcccagctca tctcttcttg agcgctaatt tctccgatac tttctacaaa cccccccacc
     8401 tttactccaa agtgacggag aaaaatctta gccactgctc ctgccacgca tttccctacc
     8461 gtttctcgag cgctggctct ttcaataaca ttccgcaggt cagaaaaacg atacttcacc
     8521 attcccccca aatcggcatg gccgggacga ggaatggtaa tctcttcgta ggaagaagga
     8581 gcgctacctc gagggtccat aagggtagac caattctcat aatctcgatt tttaacctca
     8641 aaaaccaccg gacctcctaa ggttttcccc caccgcactc cgcctaaaat ctctacttcg
     8701 tctttttcta tcttcatacg ccctcccgag ccaaaaccgc tctgacgtcg ggaaagctcc
     8761 cggtttatga aaggaatatc cacttccaga ttagcaggaa gccctctaat tagagttata
     8821 atggccgggc catgagattc accggcggtg tgaaactcca gcaatacttt cctccttaag
     8881 atgtgctatt tcctgaagaa gagataacag ctcttcttct tcgctttctt ttataatttc
     8941 tatcatttta tcaagttctc tctggaaaag accaatcgtc ttcaatattt tatctcgatt
     9001 ggtgataaaa atatccctcc acatccgggg agaagaaccc cctattctca cccagtctcc
     9061 gaaagaagga ccagcatatt gaggcaagtc ctccctatga gataaaactg ccgaaagaaa
     9121 acaattggaa agaatgtgag gaaggtgact ggtcatagcg catatttcat cgtgttcttc
     9181 aacggttaaa aaaaaacctg cccttccaga aaacgggcta tctcttccac catgttttta
     9241 ccactttctg acgccggagg gggagaaacc agaatcggca catttcttaa aatccccttt
     9301 ctggcatgtt ctattccact cctctcccca ccccccatag gatgaaaacc tgaatactcg
     9361 aggccctgaa gagcaggaga tatctctccc catacccact ctctggtgct tcctaaatct
     9421 aaaactcggg tagaaggaga aaggtgggga cgaagttcca aaaagacagg aggcacactc
     9481 ataaccggaa gagataaaaa aaccagatcc actccctggg cagcttcttc taaagaagga
     9541 gagataaaat tcactgcccc caattccttc gctttttctg ctgcttttcg gtctatatca
     9601 tagcctttaa taaactttgt gcgaggccag ctggataatt ccaggcccag agaacctccc
     9661 attaatccca gaccaatgat agcaatagct aactcttcca aactaaacct ccttttccat
     9721 caccactgcc agcttcttta tctctctcac cagatgataa aattgttcag gagtaaggga
     9781 ttgaggacca tctgataatg cctcaacggg gttgggatga acttcaacta ttacaccatc
     9841 agctcccgca gccagcgcag ctaagctcat agtttctacg agatcgcttc tccctgtgcc
     9901 atgactgggg tcggctaaaa tgggaagatg gctctgcatt tttaccaaag gaatacagct
     9961 caggtctaaa gtgttacggg tggagggctc aaaagtcctt attcctctct cacataaaac
    10021 tacctgatgg tttccctgag ccagaatata ttcagcagac atcaaaagtt cttccacggt
    10081 attcatcatg cctcgcttga gaagaacagg tttatgcagt ctgcctgctg cctcgagaag
    10141 gcgaaaattt tgcatattac gggctcctat ctgaatcata tcggcatatt cagcaaccag
    10201 aggtagctcc tccactccta aagcttcagt aactatgggt agcccgctca cttcccgagc
    10261 tttagctaaa aattttaacc cctcttctgc taatccttga aaactataag gagaagtgcg
    10321 aggtttaaag gcacctcccc ggagaatttt tgctcccgct tcttttatcc ggagagcagt
    10381 ttccacgatt tgttcttctg attctaccga gcagggaccg gctatcaata cgaattttcc
    10441 catccctatt tctacatcgc ccacttttac tgtggtatcg gcctctctga attctcggct
    10501 ggttaatcta taaggagcaa gaactggtat taccttttct acaaaaggca agaccccgat
    10561 tttttcttct aagttaactc tccgctcatc gccaatagca ccgattatgg ttctctctac
    10621 tccttgagaa acatgagccc caaaaccaag cttttccagt ctttttacta catcatcaat
    10681 ctgctcttga gttgcaccgc tccgtaacac aataatcatc gtttttctct cctaaccata
    10741 aatacgataa agttctccac aagccgcctg gatatcctgt cctcgcggtt ttcttatcgt
    10801 cacctgtatc ccgttttcct ccagaaccga agcaaaggct tttaccctcc tcggagaagg
    10861 tggagaaaaa gcagaagagg gaaccaggtt accaggaata agattaacat gaatcagtaa
    10921 tttcttcaat aatttcgcca cttcctgagc atcttcttta ctgtcattta tctcgttcca
    10981 gagagtatat tctatggtca ctcgccgatg ggtaacagcc agatattctt ttatcgccct
    11041 taaaacctgc tctaaaggat aaactttatt gagaggtatc aacaaattgc gtaattcatc
    11101 ccggggagca tgcatcgacc aggccaggtt aacctctggc caatctcttc ccaattttac
    11161 gattttatct ggtatgccca ctgtagagac ggtcattctc cttgctcctt tgcggaaacc
    11221 ttccggagaa tttatgactt gaagcgcccg atgtacattc tcataattga gcaggggttc
    11281 tcccatcccc ataaatacta agttgttagc cgtcatcccc gtttcttttt ctattaccca
    11341 tatctcttct actatttcct gataggtaag atttcgccgg aacccagcaa ggccggtagc
    11401 acaaaaagtg cagcctgccg gacaacccac ttgagtagaa agacaaagag ttcggcggtc
    11461 tctgtggggg atgagcactg cttctacatt atcttgagga ggaaaatgga ttaaaaattt
    11521 taccgttcca tctttcgatt tctgaatctc tttaatttga gagctaccca gagaaaaagc
    11581 gctatccagc ttttctcgca aggccacagg caagttggtc atatcaaaaa aggagtctgc
    11641 tttcttttca tatatccact ggaaaatttg tcgagctcga aaagaaggtt ctccctcctc
    11701 ttccagccag gagtagatat cagaaggcaa aagtccaatc agatttttct ttctcatcta
    11761 ttatgattaa taaccccgct ttttatttga gtcaggatag cataataatt cgatagtata
    11821 tgcgatatat agcttttcat gtaagaatac cagatatttt aaaatcaaac aaggaaaaca
    11881 atacaaagtc tgcactcacc ctgtgtccca cagaacaagc acaaaaaact acaattaagg
    11941 aatccttcaa atttccccca aatagccatt ggcggagagg gtgggattcg aacccacgag
    12001 accgctcagc acggcctact cgat
//
LOCUS       DAOPSS010000043        11917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4535_length_11917_cov_8.966279, whole genome shotgun sequence.
ACCESSION   DAOPSS010000043 DAOPSS010000000
VERSION     DAOPSS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11917
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4535_length_11917_cov_8.966279"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1656)
                     /locus_tag="PKN31_03980"
     CDS             complement(136..1656)
                     /locus_tag="PKN31_03980"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOA99116.1"
                     /translation="MALVGIDVGTSGCKVVAFTEGGEILASGYEEYPFLTPRPGWLEL
                     DPEKIWEAVINSLAKVVEQTEDAIDAIAVSSHGETLIPLGKDGQVLYGAIANFDTRAE
                     GYVDFWREHLNPFQIFQITGMPLHGMYTVNKILWLKDQRPDIFEKVDKFCCVEDFVLH
                     RLTGEEPVIDYSLAARTMMLDVVQKEWSDDILRLASLDKDRLPRLLPSGEEVGHLRSS
                     LAQDLGLSSSVVVATGGHDQPCGVLGCGVRKEGEAMYGMGTSDCVALNLGRKPLLTQE
                     MMKNGFCCYPHVVENSYLTLSYIASGGSVLRWFRDTFGGEEKEIAKREGKDPYQVLID
                     TIPSHPASLFLLPHLAGSGTPYLDGYSRGALLGLTLGASKGEVVRAILESITYEMKIN
                     LDLWEEFGIPLYDLRAVGGGARSTCWLQIRADILQKPLLPLFTEEAVALGSAMLGGKA
                     RGIFSNLDEAVAAMVKFGKEISPSPDKKEAYQIRYQIYRKIYGAVREIHEEISKLL"
     gene            complement(1616..2812)
                     /locus_tag="PKN31_03985"
     CDS             complement(1616..2812)
                     /locus_tag="PKN31_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA99117.1"
                     /translation="MESKTRLYLIIFLAFYFLAVSMIITGAVIPQWMDEFSVSAGRAG
                     RLFAFYYLPYTIVTFCSGLLSQRWGKKVVLLLGQAFLAGGFFSVSTSPSFSLIEWGLF
                     LMGVGGGFSEAPFTSLLSQIFPGEEGYALNLSQISFGLGAATAPFLAGFLLERGFSWR
                     LFYLFPGMVSLVIFALLSREKLLEEKGMEGGGKSPLRRREGNGFIIVSFLAMFLYVGA
                     EIGSSSWITTYLVKGLGGSLSWGGLALAVFWGALTIGRFVFAFLSRSLSYPVILRFSS
                     FLSLIFLFFLNFTHEVHWAILALGGVGFGYSAVWPLIVAWVAQKMGEEQEKAIGFVVA
                     FGGLGAIFFPWLGGVIGERWGLSYIFWVVWVLVLFLWLVFHSSYFREGGKEVGAGRDR
                     CGNEWV"
     gene            complement(2802..>3527)
                     /locus_tag="PKN31_03990"
     CDS             complement(2802..>3527)
                     /locus_tag="PKN31_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOA99118.1"
                     /translation="VKIDTAVTGEVAHKFNLLLDPDPGMRRSYLRWYEKMVRAASLLG
                     AEGSGIYMGSLSNKDVNDPERKAYLTEVLIEEIAYLTFVAREAGQSYLLWEPMSLPRE
                     MPSTIDETKELWERVNEKSHIPVLLCLDVGHGYYRSSDPRDLDYYSWLQELAHLSPSI
                     HMQQTDRKGSRHWTFTEENNAQGVIVPERVLEAIEASGAEEVVLVFEFFFSAHALSDE
                     SALDDMKKSVDYWREALVRFYGK"
     assembly_gap    3528..3627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3628..5519)
                     /gene="pdhA"
                     /locus_tag="PKN31_03995"
     CDS             complement(<3628..5519)
                     /gene="pdhA"
                     /locus_tag="PKN31_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOA99119.1"
                     /translation="MESKDKFLNMYRDMVRIRRFEETVSDLFSQDRIRGTTHLYIGEE
                     AVAVGACNAINPDDYITSTHRGHGHCIAKGATLDKMMAELFGKITGYCKGKGGSLHIA
                     DLNAGNLGANGIVGGGIPIAVGSGLTAKYKNTGKVTVCFFGDGAANTGAFHEALNLAG
                     VWKLPVIFVCENNLYAMSVPVKEAFPIQDIVERAPAYGMPGVIVDGMDVLAVKEAVEE
                     AAKRARRGDGPTLIECKTYRYLGHSKSDPRAYRTKEEESQWKERDPIKRYKQWLLGNG
                     VTEEEIKKIDEEVEKEIEEAVDFAEKSPYPPLEEITKDVYVEEDFAELERKKGTKVVN
                     GIDEWDGEMRTITYREALNEALREELKRDENVVLMGEDIALYGGAYGVTRGLWQEFGG
                     ERVRNTPISEAAIVGCCVGSSITGLRPVGEIMYIDFIGLCMDQLVNQGAKIRYMFGGK
                     ARVPMVIRTEGGCGRSSGAHHAQSLESWFLHTPGLKVVMPATPFDAKGLLKAAIREDN
                     PVLFIEHKMIYNSQGPVPPEEYIIPLGVADVKREGKDVTIIAYSRMLLFALQAAEMLS
                     QEGIEAEVIDPRTLVPLDIDTITESVRKTNRVVIVHEGCKTGGAGAEIGMKIQEEVFD
                     YLDAPIV"
     gene            complement(5522..6571)
                     /locus_tag="PKN31_04000"
     CDS             complement(5522..6571)
                     /locus_tag="PKN31_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HOA99120.1"
                     /translation="MKAAVFYGPGDIRIEERSMPSPGEGEILLRVGACAICGTDVRIF
                     RHGHHNVHPPQITGHEIAGVIADIGKGVRGYKEGERVAVAPILHCGECFYCRRGLPNL
                     CLNFEALGYHHPGAFAEYMIIPERALRGGNINRIPDNLSFEEASLAEPLACALNGELL
                     SQVGVGDYVLVVGAGPVGCMHITLSRALGASKVIVSEIQEKRLNLSREFGADYYINPE
                     KENLKEKLLQITEGIGPTVVIVAAPARKAQEEALDLAASRGRINFFGGLPGDNKIAQL
                     DSNLIHYKELFVHGTSGSTPLHNAWALEMMAAGRVEGKKLISKVVSLDELPQALEEAE
                     SGDYLKIVVKPGIGG"
     gene            complement(6592..7758)
                     /locus_tag="PKN31_04005"
     CDS             complement(6592..7758)
                     /locus_tag="PKN31_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOA99121.1"
                     /translation="MKKGIQFGAGNIGRGFIGHLLWESGLEIVFVEAREDLVRLLNER
                     GCYPLRLLHREGKEEDLIIDNLRALSTQEEDKVAKEIEEGDVVFTAVGVKNLPAIAPL
                     LAQGLELRLRGSADFLNIFLCENLKDAPQLLKEEIEKYLTPSGKNFLEEKVGLVGTVV
                     ARMVPVMGERYGVEDPLFVVAESYHHLPFDAQAVKGEVPKIAGLRAVSNFPAEVERKL
                     FIHNLGHAALAYLGYREGYTYIHEAIKDEKIRKILDGAWEEVSRALLQKHPDLDSQEH
                     EKLVEDLQERFANPLLLDTLERVGREPLRKLAPEDRLIGGANLCLSQGIFPHYIAIVC
                     GAALSYDCPRDEEAVKLQKMIQEKGVEKAMEEICGIKAESRLGQAILSAYENFS"
     gene            complement(7755..8705)
                     /locus_tag="PKN31_04010"
     CDS             complement(7755..8705)
                     /locus_tag="PKN31_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOA99122.1"
                     /translation="MSEITCIGILVADLVGRPIDRFPEKGKLMLVPEMELHAGGCAHN
                     TGVDLKKLGEEVKVVGKVGRDGLGEFMINSLRAHGIDAGGISEDDEHHTSATMVIVDG
                     QGERTFLHYPGANRGLREKDIEDSFLSGSQIVHIAGSFLMPGFDGEETARVLERAKNL
                     GALTSLDTAWDDTGKWLSTIEPALSYIDIFLSNRDEAHHISGKTNLPDIARFFMDYGI
                     KVVAIKMGEEGSFIMTEEEKVLVPPFSVVAVDGTGAGDAFCAGFLVGYLRGWDLYETG
                     RFANACGAMCVRKMGATEGVGSLEETLAFIEEAEGKGNSR"
     gene            complement(8698..9651)
                     /locus_tag="PKN31_04015"
     CDS             complement(8698..9651)
                     /locus_tag="PKN31_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOA99123.1"
                     /translation="MNNSDLELITRVAWLHFMEGMTQREIARHLNISQPKVARLLDRA
                     QKNGIVKISIESPLANCLTIEKKLQELFPSLEDSLVVPASPRQDLLETLGRAGAWYLE
                     RVLSDGDLVGIAWGRTLKQVALSIKSAPVKNLKFVTLVGGLTASASLNPYTIGERLAS
                     IFEGECYYLYAPAVVESEEIRDFYLRERINQKTLDLARQAKWSLLGIGAVDPRYSIYA
                     LTGFIDYHEWELLLQKGAVGDMVGQFYNREGEIIDTPLHRRTVAIPLEDIRHMPRVIG
                     IAGGDNKVEAILGALRGGFINILITDEETALKIIERGEKNE"
     assembly_gap    9736..9835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9866..10597)
                     /locus_tag="PKN31_04020"
     CDS             complement(9866..10597)
                     /locus_tag="PKN31_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOA99124.1"
                     /translation="MKNEEGGGILEGIFNFFLPQHCLVCGSYLSRSRGFPLCSACFSR
                     IEYLRSPLCVKCGRPLPGSSALLCHHCRAFSFHFDSARAVALYTSPFRECIHAFKYQG
                     FTSFRSLFGELLCEYLEKNPFLREVDFLLPVPLHPARFIERGFNQSLILAEEIKKCFS
                     LPLVSKEVRRVKPTLPQVGLRFRERRKNMRGAFEIKENNLFQNKRVLIVDDVLTSRST
                     VDSLSQTLKKGGAKEVLVLALASGK"
     gene            10730..11710
                     /locus_tag="PKN31_04025"
     CDS             10730..11710
                     /locus_tag="PKN31_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOA99125.1"
                     /translation="MSTEKPYNLPSGNREETLLRRYEKNPILKPEDWPYEVNSTFNPG
                     ACIFQDKVLLLVRVEDRRGFSHLTVATSDNGRDNWVIEKEPCLFPEDYAHEEQWGIED
                     PRIVFLEEEKRYAVTYVSFSKGGPVVSLALTDDFKHFEKKGGLLPPEDKDASLFPRKF
                     KVGSSSPRYALIHRPIIRGEAHIWLSFSPDLRHWGDHQILIPTRGGLWDQHRVGLGAQ
                     PIETPEGWLIFYHGVRATASGNLYRAGAALLDLEDPTRVLYRTSEWLLSPKEPFEWLG
                     DVPGVVFPSGAILNEEKNEILLYYGAADKCVGLAIGDYQEIMENLKANPV"
BASE COUNT         3296 a         3083 c         2201 g         3137 t
ORIGIN      
        1 tgataggggg gctttctccc cctatagtcc gttattcact tttcaaggtg cgttcgtctc
       61 ttttccaatc tcccagtaca cctatattgt accccttgtt tttttgtaaa aaggggcttg
      121 gagagtttgt ctactttata acagtttact gatttcttcg tgtatttctc tcaccgctcc
      181 ataaatcttc cgataaattt gatatctgat ctgataagcc tcttttttat ccggagaggg
      241 agaaatctct ttcccaaatt ttaccatggc cgccacagcc tcatccaggt tagaaaatat
      301 accccgggct tttcctccta acatagcgct tcccagagct actgcttctt cagtaaaaag
      361 gggaaggaga ggtttctgca aaatatcagc tcgaatctga agccagcagg tagagcgagc
      421 tcctcctccc actgctcgta aatcatatag aggaatgcca aattcctccc ataagtccag
      481 attaattttc atctcatagg taatactttc taaaatagcc ctgactacct cacccttgct
      541 tgctcccaga gtcaagccta aaagcgcccc tcgagagtaa ccatcaagat agggagtacc
      601 ggagccagcc aaatggggta gaagaaaaag ggaggcagga tgagaaggaa tggtatcaat
      661 caaaacctga tagggatctt ttccttctct ttttgctatc tctttttcct caccaccaaa
      721 ggtatctcga aaccagcgca aaaccgaacc acctgaggca atgtaagaga gagtaaggta
      781 ggaattttct accacatggg gatagcaaca aaaaccattt ttcatcatct cttgcgtaag
      841 aagaggcttt ctccccaggt ttaaagccac acaatctgaa gtccccatcc cgtacatcgc
      901 ttctccttct tttctcactc cacaacccag aactccgcaa ggctggtcat gacctcccgt
      961 tgctaccaca accgaagatg ataaacccaa atcttgagct aaagaagaac gcagatgacc
     1021 cacctcttct ccggagggta aaagacgagg gagtctgtct ttgtctaaag aggcaagacg
     1081 taaaatatcg tctgaccact ctttttgcac cacatccagc atcatggtac gggcagctaa
     1141 agaataatcg ataaccggtt cttcaccggt gaggcgatgg agaacaaaat cttccacaca
     1201 gcaaaattta tccactttct caaaaatatc cggtctctgg tctttgagcc agagaatttt
     1261 attcacggta tacatcccgt gaaggggcat accggtaatt tgaaaaatct ggaaagggtt
     1321 caggtgctcc ctccagaaat ctacgtatcc ctccgcccgg gtgtcaaagt tggctatcgc
     1381 tccataaaga acctgaccat ctttccctaa ggggatgaga gtttcaccgt gagagcttac
     1441 cgctattgca tctatcgcat cttcagtctg ctctactact ttcgctaaag agtttatcac
     1501 tgcctcccag attttttccg ggtctaattc taaccatccc ggtcgaggag ttaaaaaggg
     1561 atactcttcg taaccagaag cgagaatttc tcccccttcc gtaaaagcta ctactttaca
     1621 cccactcgtt cccacatcta tccctaccag cgccaacttc cttccctcct tccctgaaat
     1681 aagaagaatg aaaaaccagc cacaaaaaga gaactaaaac ccaaactacc cagaaaatat
     1741 aggaaagccc ccatctctct cctataactc ctcctaacca gggaaaaaat attgctccca
     1801 ggcctccaaa agctaccaca aaaccgatag ctttttcctg ttcttccccc attttttgcg
     1861 ctacccaggc cactatcaaa ggccagacag cagaatagcc aaaccctact cctcccaaag
     1921 ctaaaatagc ccagtgtacc tcgtgagtga agtttagaaa aaagagaaaa attaagctca
     1981 aaaaggaaga aaaacgcaaa ataacaggat aggagagaga gcgggagaga aaagcaaaaa
     2041 caaatcttcc tatggttaag gcaccccaga aaactgccag agccaaccct ccccaggata
     2101 agctgcctcc caggccttta accaaatagg tagttatcca ggaggaagaa ccaatttctg
     2161 ctcccacata aagaaacatc gctaaaaagc tcactattat aaagccatta ccttctctcc
     2221 tcctgagcgg agactttcct cctccttcca ttcccttttc ctccagaagt ttttccctgc
     2281 tcagaagggc aaaaatcacg agagaaacca ttcccggaaa aagataaaat aatctccagg
     2341 aaaagcccct ttctagtaaa aacccagcca gaaatggagc agtggctgcc cccagaccaa
     2401 aagaaatctg ggataaatta agagcataac cttcttctcc cgggaatatt tgagaaagaa
     2461 gagaagtaaa aggagcttca gaaaatcccc ctcccactcc cataagaaac agtccccatt
     2521 ctatcaagga gaaggaagga gaggtgctga cggagaaaaa tcccccggcc aagaaagcct
     2581 gccctaaaag caaaaccact tttttgcccc accgttgaga aagcaagcca ctacaaaaag
     2641 taactatagt gtagggaaga taataaaagg caaagagccg gccggcccgg ccggccgata
     2701 ccgaaaactc gtccatccac tgaggaatca ctgctccagt tatgatcata gaaacagcca
     2761 gaaagtaaaa ggctaagaaa atgattaaat ataaccgagt tttactttcc atagaaacga
     2821 accagggctt ctctccagta atccacgctc tttttcatat catctaaagc gctttcatcg
     2881 cttaaggcgt gggcagagaa gaaaaattca aacaccaaaa ctacttcttc agcacctgaa
     2941 gcttcaatgg cctccagaac tcgctcggga acgattactc cttgagcatt attttcttcg
     3001 gtaaaagtcc agtgccggct cccctttcgg tcggtctgct gcatgtgaat agaaggagaa
     3061 agatgggcca gctcttgcag ccaggaataa taatccagat ctcgagggtc tgaggagcga
     3121 taataaccat gccctacatc gaggcataaa agcaccggaa tgtgggattt ttcattcact
     3181 cgctcccaca attctttagt ctcatcaatg gtagaaggca tctcccgggg aagagacata
     3241 ggttcccaca aaagataact ttgtcccgct tctcgagcaa caaaggtcaa ataagcaatt
     3301 tcttctataa gcacctcagt tagatatgct ttcctctcgg gatcatttac gtctttgttg
     3361 ctcaaactcc ccatataaat acctgaaccc tcggctccta aaagagaagc cgcccgcacc
     3421 attttttcat accaccgcag atacgagcga cgcatacccg ggtcaggatc taaaagtaaa
     3481 ttgaacttgt gagccacttc cccggtaacc gccgtatcta ttttcacnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnact ataggggcat ctaagtagtc gaaaacttct
     3661 tcctgaattt tcattcctat ttctgcccct gctcctccgg ttttgcatcc ctcatgaacg
     3721 ataactaccc gattggtttt ccgaactgat tcagtgatag tgtctatatc aagaggcacc
     3781 agagtgcgag ggtctatcac ttctgcttca atcccttcct gagagagcat ttctgctgcc
     3841 tgaagggcaa aaaggagcat tcgagaatag gcaatgatag ttacatcctt tccttctctt
     3901 ttcacatctg ctacccctaa aggaattata tactcctcag gaggaacagg gccttgactg
     3961 ttataaatca tcttgtgctc aatgaaaagt accggattat cttcccgaat ggcagcttta
     4021 agcaaccctt tggcgtcaaa aggagtggca ggcatcacca ctttgagccc cggagtatgt
     4081 aaaaaccagg attccagact ttgggcatga tgagcgcctg aagagcgacc gcatccgcct
     4141 tcagtgcgga taaccatagg aacccgagcc tttcctccaa acatataccg gattttggct
     4201 ccttgattca ccaattggtc catgcacaaa ccgatgaaat cgatatacat gatttctcct
     4261 accggtcgca atccagtgat ggaagacccc acacagcaac ccacaatggc tgcttctgat
     4321 ataggagtat tacggactct ttctcctcca aattcttgcc atagccctcg agttaccccg
     4381 taagctcccc cataaagagc aatgtcttct cccatcaata ccacgttttc atctcgcttt
     4441 aactcttctc gcaacgcttc gttgagcgct tctcgatagg ttatggttct catttctccg
     4501 tcccactcat ctatcccgtt aactacttta gttcctttct ttctctctaa ttcggcaaaa
     4561 tcttcttcta catatacgtc tttggtgatt tcctccagtg gtggataggg acttttttcg
     4621 gcaaagtcca ccgcctcttc tatctctttt tccacctctt catcaatctt ttttatctcc
     4681 tcttccgtta ccccatttcc caggagccac tgtttatatc gtttaatagg gtctctctct
     4741 ttccattgac tttcttcttc tttagttcgg taagcccggg ggtcactttt ggagtgccct
     4801 aaataacgat aggttttgca ttcaattaaa gtaggaccat cccctcgccg ggctcttttg
     4861 gctgcttctt ccacggcctc ttttaccgct aaaacatcca tgccgtctac tattactccc
     4921 ggcattccat aagccggggc tctttccaca atatcctgaa tgggaaaagc ctccttaacc
     4981 ggcaccgaca tagcataaag gttattttcg cagacaaaaa ttaccggcag tttccatact
     5041 ccggccaaat tcaaggcttc gtgaaaagcc ccggtattag ctgctccatc accaaaaaag
     5101 cacactgtta cttttcccgt atttttatat ttagcagtca accccgagcc cacagcaatg
     5161 ggaatccctc cacccacaat accattagct cctaagtttc cagcgttaag gtcagcaata
     5221 tgcaatgacc cacctttgcc tttacaatag ccggtaattt taccaaagag ctcagccatc
     5281 attttatcta aggtagcccc tttagcaata cagtgcccgt gaccccggtg agtagaagta
     5341 atgtaatcat cggggttaat agcattacag gctcctactg ccactgcttc ttctccaata
     5401 taaagatgag tggtgccccg aatcctatcc tgactgaaga gatcactcac tgtctcttca
     5461 aagcggcgaa ttcgcaccat atcccgatac atattaagaa acttatcctt ggattccatg
     5521 tctatcctcc tattccaggt tttaccacta tttttaaata atctccactt tctgcttcct
     5581 caagagcctg aggaagttca tctaaactta ccactttact gatcaatttt ttcccttcca
     5641 ccctacctgc cgccatcatc tctaaagccc aggcattatg aaggggagta gaaccagaag
     5701 ttccatgaac aaaaagttct ttataatgaa tgaggttact atctaattga gctatcttgt
     5761 tatccccggg aagacctcca aaaaagttaa ttcgcccccg ggaagcagcc agatccagag
     5821 cttcctcctg agctttccga gctggagcag caacgattac cacagtagga ccgatgcctt
     5881 cggtaatttg gagaagcttt tcttttaggt tttctttctc cggattaata taatagtcag
     5941 cgccaaactc ccgggacaaa tttaatctct tttcttgaat ttccgaaact attactttac
     6001 ttgcccctaa agcccgagat agcgttatat gcatgcagcc tacaggacct gcccccacca
     6061 ccaaaacata gtctcccact cctacctgag agagtagctc cccattcaaa gcgcaagcca
     6121 gaggttcagc taagctggct tcctcaaaag acaagttatc gggaatacgg ttgatgttcc
     6181 ctcctcgcag cgctcgctcc ggaattatca tgtattcagc aaatgccccg ggatgatgat
     6241 accccagagc ttcaaagttc aaacacagat taggtaaccc tctccgacag taaaaacatt
     6301 ctccacagtg taatatagga gctacagcca ctctttctcc ctctttatat ccccgtaccc
     6361 cttttcctat atcggcaatc actccggcga tttcatgccc ggtaatctgg ggaggatgaa
     6421 cgttatggtg gccatggcgg aaaatacgca catcagttcc gcaaatagca caggctccca
     6481 cccggagaag aatctcccct tccccgggag aaggcataga tcgttcttct atccttatat
     6541 caccgggccc ataaaatacc gctgctttca ttttttccac tcctttcttt ttcaagaaaa
     6601 attttcataa gcagaaagaa tggcctgacc taacctgctc tctgctttta ttccacatat
     6661 ctcctccata gccttttcta cccccttctc ctgaatcatt ttctgcaact tcaccgcttc
     6721 ttcatcccga gggcagtcgt aggaaagagc agccccgcaa actatagcta tatagtgagg
     6781 gaaaattcct tgcgaaagac acaggttcgc tccccctatc aggcggtctt caggtgctaa
     6841 tttccgcaaa ggctctctcc ctactcgctc taacgtatct aaaagtaagg ggttggcaaa
     6901 tctctcttgc aagtcctcca ctaacttctc gtgctcttga gagtctaaat cagggtgttt
     6961 ctgcaaaaga gcccgagaaa cttcctccca ggccccatcg agaatttttc ttatcttttc
     7021 atcttttata gcttcgtgaa tataggtgta accttcgcga tatcctaaat aagctaaagc
     7081 agcatgacct aaattgtgaa tgaataactt tctttccact tccgctggaa aattgctcac
     7141 cgctctcagc ccggctattt tcggcacttc tccttttact gcctgagcat caaaaggcaa
     7201 gtgatggtaa gattccgcca ccacaaaaag agggtcttct accccgtagc gctctcccat
     7261 aaccggcacc attcgagcca ctactgttcc cactaacccc actttttctt ctaaaaaatt
     7321 tttaccggaa ggagtgaggt atttttctat ctcctccttc aatagctgag gagcatcttt
     7381 taagttctca cagagaaaaa tgtttaaaaa atcggcactc ccccgcaagc gaagctccaa
     7441 cccttgagct aaaagaggag caatggcagg aagattcttc actcctacag cagtaaatac
     7501 cacatctcct tcttctattt ctttagctac tttgtcctct tcttgagtag aaagggctct
     7561 caggttatct atgattaaat cttcttcttt cccctcgcgg tggagaagac gcaagggata
     7621 gcatccccgc tcattcaata accggaccag gtcttccctg gcttccacaa aaacaatctc
     7681 caatcccgat tcccagagca agtgaccaat gaatcctcgt ccgatgttac ccgctccaaa
     7741 ttgtatccct tttttcatct actgtttcct tttccttcgg cctcttcaat aaaagccaga
     7801 gtttcttcca agcttcccac tccttcggtg gctcccattt ttctaacaca catagctcca
     7861 caagcattgg caaagcgccc agtttcatag agatcccagc ctcgtagata ccccaccaaa
     7921 aatccggcac aaaaagcatc acccgcccct gttccatcta ctgctactac cgaaaaagga
     7981 ggaactaaaa ccttttcttc ttcagtcatg ataaaacttc cctcttcccc cattttgatg
     8041 gccaccactt ttatcccgta atccataaaa aaccgagcaa tatcaggaag attggttttc
     8101 ccggatatat ggtgagcctc atcacgattg gagagaaaaa tatcgatata agaaagagcg
     8161 ggttcaatag tggagagcca ctttcccgtg tcatcccagg cagtatccaa agaagtaaga
     8221 gctcccagat tttttgctct ctcgaggact cgggcagttt cttctccatc aaatccgggc
     8281 atgagaaagc ttcccgctat gtgcactatt tgtgagccac ttaaaaaact atcttctata
     8341 tctttttctc tcaagccccg attggcccct ggatagtgaa ggaaagttct ctccccctgg
     8401 ccatccacta ttaccatggt ggctgaggta tgatgctcat cgtcttctga gattcccccg
     8461 gcatcaatcc catgcgcccg cagagaattt atcataaatt ctcccagacc atctcttccc
     8521 acctttccca ctaccttaac ctcttctccc agctttttta aatctactcc ggtgttgtga
     8581 gcacatcccc cagcatggag ttccatctcc ggaaccagca ttaattttcc cttttccggg
     8641 aaacggtcaa ttggacgtcc tactaaatca gctactaata ttcctatgca ggttatctca
     8701 ctcatttttt tcccctcttt ctattatttt taaagcggtt tcttcatcgg taataagaat
     8761 atttataaaa ccaccccgca aagcccccag gatggcttcc accttattat cccctccggc
     8821 aataccgatt acgcggggca tatggcgaat atcttctaag gggatagcca cagttcttcg
     8881 gtgcagagga gtgtcaataa tttctccctc ccggttataa aattgaccca ccatatctcc
     8941 tactgctcct ttttgaagca agagctccca ctcatgatag tcaatgaagc cagttagagc
     9001 ataaatagag tagcgagggt ccaccgcccc gatacctaaa agagaccact tagcctggcg
     9061 agccaagtcg agagtcttct ggttaattcg ctctcgcaga taaaaatcac gaatttcttc
     9121 tgattccacc actgctggag cgtaaagata gtagcattct ccctcaaaaa tagaagccaa
     9181 tctctctccg atggtataag gattgagcga agctgatgct gtcagccccc ctactaaagt
     9241 aacaaacttg aggtttttaa cgggagctga tttaatagac aaagccacct gttttaatgt
     9301 cctaccccag gcaataccca ccaaatctcc atcgcttaga accctctcca gataccaagc
     9361 tcccgctcgc cctaaagtct ctaacaagtc ttgccggggg gatgcaggaa caaccagact
     9421 atcttcgaga gaaggaaaaa gctcttgcag ttttttctct atcgtaagac agttagccag
     9481 aggagattcg atagaaattt ttactatccc gtttttctgt gcccggtcaa ggagacgagc
     9541 tactttgggt tgcgaaatgt ttagatggcg agcgatttct cgctgagtca ttccttccat
     9601 aaaatgaagc caggcaacgc gggttatcag ttctaaatcg gagttattca tttctttcct
     9661 tttttgaatt gatattcaaa aatagaataa tctttcaata acattgtata acaaatttaa
     9721 atttaatcaa gaattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaat
     9841 atatttgctt ttgaagaata aaaattcact ttccggaagc cagagccaga actaaaactt
     9901 cttttgcccc tccttttttt aaggtttgac ttaaactatc caccgtagag cgagaagtga
     9961 ggacatcatc tactatgaga acccttttgt tctgaaaaag gttattttcc tttatctcaa
    10021 aagcccctcg catgttcttt cttctttccc tgaagcgtaa gcccacctgg ggtaaagtag
    10081 gtttaactct ccggacttct ttagacacaa gaggaaggga aaagcatttt ttaatttcct
    10141 ccgccagaat aagcgattga ttaaaccctc gctcgatgaa gcgagcagga tgaagaggaa
    10201 caggtaaaag aaaatccact tctcttaaaa agggattttt ttccagatat tcgcaaagga
    10261 gctcaccaaa aagagagcga aaagaggtaa agccttgata tttaaaagcg tgaatgcatt
    10321 ctcgaaaagg agaagtgtaa agcgccactg cccgggcact atcaaaatgg aaagaaaaag
    10381 ctcgacagtg gtgacacaaa agagcagaag agccaggtag aggtctgcca cacttcacac
    10441 ataaaggact gcgaagatac tcaatccggg aaaaacacgc agaacacaga ggaaaacccc
    10501 gggagcgaga aaggtatgag ccacaaacca ggcaatgttg aggaagaaaa aaattaaaga
    10561 taccttctaa aattcctcca ccttcttcat ttttcatata ttttcttccc ggagaaaaac
    10621 taaagttatt ttttgaatta tagcagaaat tgcccttctt taagcacttt ttggacttat
    10681 cttaaaatta ctttataata aaagaaatca agctggaaag gagaacaaga tgtccaccga
    10741 aaaaccgtat aaccttccct cgggaaacag ggaagaaact cttcttcgtc gatatgagaa
    10801 aaaccccatc ttgaagcccg aagattggcc ttatgaggtt aattccactt tcaatcctgg
    10861 agcctgtatc tttcaggata aagtcctcct ccttgtgcgg gtagaagacc gccgggggtt
    10921 ttctcatctc acagtggcca cgagtgataa cggtagggat aactgggtta tagaaaaaga
    10981 accctgtctt tttcctgaag attatgccca cgaggagcag tggggaatag aagacccccg
    11041 tattgttttt ttagaagaag aaaaaagata tgccgttact tatgtctctt tcagcaaagg
    11101 agggccggtg gtttccctgg cacttaccga cgacttcaag cattttgaaa aaaaaggagg
    11161 gcttctccct cccgaagata aagatgcctc cctttttccc agaaagttca aggtgggctc
    11221 atcttcacct cgctatgctc tgattcatcg ccccatcatt cgaggagaag cacatatctg
    11281 gctttctttt tctcccgatc tccgacactg gggcgatcac caaatcctca ttcccacccg
    11341 gggaggattg tgggatcaac accgggtagg acttggtgct caaccaatcg aaactcctga
    11401 aggctggctt atcttctacc atggagtgag agccaccgct tcaggaaacc tctatcgagc
    11461 aggagctgct cttctggact tagaagaccc cacccgggtg ctttaccgaa ccagcgagtg
    11521 gctactgagt cctaaagaac cttttgagtg gttgggcgat gttcctggtg tggttttccc
    11581 ttccggagcc atactgaacg aggaaaagaa tgaaatcttg ctctactatg gagccgccga
    11641 taaatgcgtg gggctggcca ttggtgatta tcaagaaatc atggaaaacc ttaaagccaa
    11701 cccggtatag cattctacca ggatgcggga attttccatt ttaatcctga tttaagctaa
    11761 tcctttttat cctgtaaaag tggatggtcg cttttcttta cttcttatgt gaaaagatgg
    11821 cctaccacgt gaccatccac tttttgtttt acgaaaacat cgaatacaac cacagagaag
    11881 ctttctgaaa gtgacactgt aatcaggggt ttgctcg
//
LOCUS       DAOPSS010000044        11629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4671_length_11629_cov_8.754018, whole genome shotgun sequence.
ACCESSION   DAOPSS010000044 DAOPSS010000000
VERSION     DAOPSS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4671_length_11629_cov_8.754018"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1173
                     /locus_tag="PKN31_04030"
     CDS             196..1173
                     /locus_tag="PKN31_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99126.1"
                     /translation="MSQPELESFIQFSNEVTIITHIIYPSFPFTLFPYLETYHPFLDR
                     SFFWSKGRWWIALGERKVEIAQVAEKGDGRNPFLEITVESEETVEEVSSILGVNEDPL
                     PFYQLGEKDPVMNEIIEEFYGARIPRSRSLFEAAVTTILEQQISMKVASVLRKRFVEE
                     WGRGPLYREGEAIFAFPASRDIVKTSPEELRKIGMSGAKARAIHRLTEMIEEKEIDPE
                     FLKTLPSSQVVDFLTHLPGIGAWSAQFIVARWMPTNDCFAPGDLGLRKAIGFFYQGSE
                     HPVSTADAQEILRRFSPYERWAGWYLLMAIEKEKGSPSRDSSFQIIEKI"
     gene            1193..1831
                     /locus_tag="PKN31_04035"
     CDS             1193..1831
                     /locus_tag="PKN31_04035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA99127.1"
                     /translation="MKDLFLHAKSGAENWGWRVFTDLLPAYFTEEHFENLYRRKQDPW
                     NYEHSGFEREKYLKTLQAIPGEVETIWEIGCGEGVFTRLLLEEGKRVGGVDISSTALS
                     RAKERLKDFGDKVCLQKLDIVREDIEGNFDLILASEILYYLGGKDILQSLEEKFFRHL
                     RPYGYLLLCHFYPSGKIIHNFYEENHRFYKVFEEITYHPHRDYIITLLKRNG"
     gene            complement(1840..3213)
                     /locus_tag="PKN31_04040"
     CDS             complement(1840..3213)
                     /locus_tag="PKN31_04040"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOA99128.1"
                     /translation="MLGILIGCFFLIKILPFNSIFAPLFHVYSKVYTKLIEPLIDDSE
                     RVLIFAPHPDDETLSCGGAIQKSLSEGKIVKVVFVTSGDSFGNDYEMTGLSEDLPRGV
                     ELGYLRQKEALEATAILGLEKENVIFLGYPDRGMEKMWWMYRSDDQAYRSPYTKTDKS
                     PYLSGYTLSAPYYGEQVIQDIQSILEVFHPQIIYLPHPSDLHSDHWATYNFVKEALER
                     LRQKGSDWIDDAKVYLYLVHFGRIKWPPLWGYTPHLRFTPPSQLMSTHQWVGFELDET
                     EIANKKKALDQYRSQQAVREFLLAFVKANEVYAIDNSYYLREGESVFIPDEKGEFTLP
                     RIINGGDIRGLDVMLEGSTVSLKLHHDSGIPPQARYRFYLIGYSEGEVAFQESYMLFD
                     RKKVVRLQGDYFSNSPVVLRGSGVVTLTFDFEHLPFPDALFLSVGSSAPQLALDRLPW
                     SMVILEK"
     gene            3644..5548
                     /locus_tag="PKN31_04045"
     CDS             3644..5548
                     /locus_tag="PKN31_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOA99129.1"
                     /translation="MKMNIRSKLTIISLIPLALFILLIFAYITPSLRDVAENVMEHSL
                     LMKLQGDMHSVHLFAEKHFGEIKMVDGALVDKDGVPLEGRHDMVDEITDKLGVVATVF
                     VREGNDFRRITTSIKTADGNRAVGTMLGTDSAAYEPVMAKNLYIGKAEILGANYYTAY
                     DPIVDKGEVVGILFIGVPTDEAESIVSSGISGTSRMIIIFSVAALIASIIIIFLLGSS
                     ISHPVQVATKQAELLREGNLSVEIPAHLLKRKDELGLLGRALKNMAEKWREVVQNVIG
                     VSSKLASSSGELSSSVEEVSKATQEIARTMTQVAEGSVRQEEDLQHLSGTVEGINKMA
                     QNIREKTQENVERLDTVMKERLNENATALKHIQEEIKKATQSGAQAGEEAQKGQELLG
                     LLMEGITAISRVTQEVAESIAALEGRSEEIGKIVDVITEITEQTNLLALNAAIEAARA
                     GEAGRGFAVVANEVRDLAEDSAQAAQQIAGLVGEIQRDTQLAVKNMERAQEEVKEGTG
                     KGETVARSFSQILKTIQGVVENINHIASSSQILEKAQKDLLSTQEETNIATSEVAKAV
                     EEISYSIGEATERVNSIVAVAEENAASSEEISASTEEQSASLEEITSAVESLAHMAQE
                     LQIMVSHFQV"
     gene            6207..7253
                     /locus_tag="PKN31_04050"
     CDS             6207..7253
                     /locus_tag="PKN31_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOA99130.1"
                     /translation="MSKRISKLLVISVICVLFLSLGSQVFAQKLPVVGISTGSSGTSW
                     RDIMIAAMQEVGDEYKAAGKIADYKIVNNIVNGDATEQANILRDFISQGMDIIMVNPN
                     SPDALNGVIKEAQEEGILVVSFDATVTAPDVVNITLDHYAWNKKNVEFIASTLQKGNA
                     VQIYGLEGHPANNERIRATYEVLANYPDIKLIADTSGYWDQTKAKEVATQIIASGQQI
                     DAVITQDGMAFGILSAFLDANKLPKVMYGDPGTAFFKEWKKLRDQGADFKACTQPNPP
                     GIGGTAFRIALQLYNGKNFKPDILKDNTFYYQVSMFVTDENFDEAWELLKDKPDDYLL
                     SEIMSEEEVAALFE"
     gene            7596..9089
                     /locus_tag="PKN31_04055"
     CDS             7596..9089
                     /locus_tag="PKN31_04055"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA99131.1"
                     /translation="MIVLEAIGIKKNFGGVVALSNANLRCYQGRITGLLGANGSGKST
                     MSKIICGVYQADGGEIKYKGRTVRYRSPSEARKDGIAMVFQNLSLVADLTVWQNIVLG
                     KEESNGIFLNNDSAKELSRKILAQLLPDLDINKKVSQLNPGEMQIVEIAKAISQNPQL
                     LILDEPTAALEQAQVKNLFDCMRALAKEGVAMIFTSHRLWEVMEICDDVTIFRNGENV
                     ATIDFEKDEKDPEKIISYITGDTQKTKVEKKHVAVTDETILSIKNLNYGKSLKNVSFD
                     LKKGEILGIGGLAGQGQLELMHALAGAFHSLHCDAELRGKKIKLNRPLNAIKEGIVFV
                     PGDKQLEGLFLKRSVLFNMIFPKMGLPGHPSFIPYDEYRRECEEIVEVLSIKTPTVDV
                     PVENLSGGNQQKVVVGKWLPFDINVLLLADPAKGIDVGAKRDLYQYIVNQVQEKNTSV
                     ILYASDNEELVEYCDRVLIMYEGQIVASLEGGDINQDTIIACSMRLR"
     gene            9103..10113
                     /locus_tag="PKN31_04060"
     CDS             9103..10113
                     /locus_tag="PKN31_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99132.1"
                     /translation="MNKNNQNSSRPALSNYVFRPEFSSLLILIVMFTITAILQENFFT
                     LKSIVRNINAFAPLILLAMGAAVVIISGGIDLSAGSALSLFTCVLTTVMKKSDPITGV
                     YAILVTLAVAILVGVVNGFGIGYLRIPPVIVTFATSYIWLGIGLFLRPTPGGESVEWF
                     RIFYNPGMIEGIHPYIKQFGRVIPPALILILAACLIWFIVSKTRIGRHIYAVGSNSES
                     AYASGINTAKVQMQACIINSIFIFLAALFFVGQNQSGDARMGDPLTLRAIASAIVGGV
                     AISGGKGSVYFALVGALILNFVNKIIFFADISTAYQTLVSGLIVIIAIAAPVAYTLYS
                     KE"
     gene            10133..11095
                     /locus_tag="PKN31_04065"
     CDS             10133..11095
                     /locus_tag="PKN31_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99133.1"
                     /translation="MNLGISMFFERYKKIIVAFILIVLLFALGGIIVPNFLTVEQVLF
                     TLKLASFTALFALCQMIVIAAGGSGLDLSVGYAATLTAVLTASVMDGQNANLGAAILI
                     ALALGSAIGLANGALVSFLELPPLVVTMAMANILQGIVNAYTAGRNITGRPSPILATL
                     AAKSTGKFPNILFILFTITVLVMIVLNKTKWGTILFGVGSNETTAYLSGINVKAVRCV
                     VFVISGILASLTGLLLIGNMGIAFKDMASNYVMPSIAAVVVGGVSLTGGDGNYIGVIL
                     GAIFLQALTNLLIAFGLGDAGKWLAFGIVLYVLLSFYASDRRSR"
     gene            complement(11439..>11629)
                     /locus_tag="PKN31_04070"
     CDS             complement(11439..>11629)
                     /locus_tag="PKN31_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOA99134.1"
                     /translation="GKEKASPKEIREVLNSLNFAEVEIENQQFLLKIKGTELSNKILK
                     LFKIKPPKNITPKEELSL"
BASE COUNT         3570 a         2309 c         2613 g         3137 t
ORIGIN      
        1 ataacttggc tcaaaaaata gggatttact atctgaaggc gataagcctc ttgctgagca
       61 cttccgcctc ataaaccgga ggaatggcaa agaaactaag gtatacgata tagatggaca
      121 catcaaaagt tagaggttat actataattg tcttaaaacc gcaagctgag aggacagttt
      181 caatgcggaa tagaaatgtc tcagcctgag cttgagagtt tcattcagtt cagtaatgag
      241 gtaaccatta ttactcatat catttatcct tcttttcctt ttactctttt tccttatttg
      301 gaaacctacc accctttttt agatcgctct tttttctgga gcaaaggcag atggtggata
      361 gcactgggcg agagaaaggt ggagatagct caagtagcag aaaagggaga tggaagaaat
      421 ccctttcttg agataactgt agagagcgaa gaaaccgtag aggaagtttc ttccatatta
      481 ggtgtcaacg aagaccctct tcctttttac cagttagggg aaaaagatcc ggttatgaat
      541 gaaatcatag aagaatttta tggagcacgg attcctcgct ctcgaagctt atttgaagca
      601 gcagtaacta ctattctgga gcagcaaatt tctatgaaag tggcctcggt tttacggaag
      661 cggttcgtgg aagagtgggg tcgaggtccc ctttacagag aaggagaggc gatttttgct
      721 tttccagctt cccgggatat agttaaaaca tctcctgagg agctaagaaa gataggaatg
      781 tccggtgcta aagcccgagc gattcaccga ttgactgaga tgatagaaga aaaggaaata
      841 gacccggaat tcttgaagac attaccctcc tctcaggtag ttgatttttt aactcatctt
      901 ccaggaatag gagcatggag cgctcagttt atagtggccc ggtggatgcc gactaatgac
      961 tgttttgccc ccggcgacct gggcctgaga aaggcgatag gttttttcta ccagggaagc
     1021 gagcatccag tctctaccgc agatgcccag gaaatactga ggcgtttttc tccctatgag
     1081 cgctgggcag gatggtatct cctcatggcc atagaaaaag agaaaggttc tccttcccga
     1141 gatagctcct ttcagatcat agaaaaaata tgatgaacat atgaaaagtg gcttgaaaga
     1201 tttatttctc catgctaaat ctggggcaga aaactgggga tggcgagtat ttactgacct
     1261 tctaccggca tattttaccg aggaacactt tgaaaatctt tatcgccgaa agcaagaccc
     1321 ctggaattat gaacactctg gctttgagag agaaaaatac ctcaaaactc tccaggcgat
     1381 accgggggaa gtggaaacta tctgggaaat aggatgtggc gagggagttt ttacccggct
     1441 gcttctcgaa gaaggaaaaa gagtcggggg agtagatatt tcctctactg ctctttctcg
     1501 agcaaaagaa aggttaaaag attttggcga taaggtatgc cttcaaaagc tcgatattgt
     1561 aagagaagat atagagggaa attttgacct cattcttgct tcagaaatct tatattactt
     1621 aggaggaaaa gatatccttc aatctctgga ggaaaagttt ttccgtcatc tgcgacccta
     1681 tggttatctt cttctctgcc atttttatcc ctcggggaaa attatccaca atttttatga
     1741 agagaatcac cgattctaca aggtgtttga agaaattact taccatcccc accgagacta
     1801 cattattact ctcctaaaga ggaacggatg acaatgccat catttttcta gaattaccat
     1861 gctccaaggg agacgatcaa gtgctaactg aggagcagaa gatccaacgc ttaaaaagag
     1921 agcatcggga aaaggaagat gctcaaaatc gaaggtaagg gtaaccaccc cgctacccct
     1981 caaaaccact ggagagttag aaaaataatc tccctgaaga cgcactactt tctttctatc
     2041 aaaaagcata tagctttcct ggaaagccac ttcaccctca ctgtatccaa tgagataaaa
     2101 acgatagcgt gcctgaggcg gaatacccga atcatgatga agtttcaaag agacagtgct
     2161 tccttcaagc attacatcta atcctcggat gtctcctcca ttaatgattc tgggaagagt
     2221 aaattccccc ttttcatcag gaataaaaac actttcccct tctctcagat aatagctatt
     2281 atcgatagca tagacctcat tagcttttac gaatgccagt aagaattccc gaactgcttg
     2341 ctggctccga tattggtcaa gagctttctt tttgttggca atttctgttt catctaattc
     2401 aaagccaacc cactggtgag tggacataag ctgagaagga ggggtgaagc gaagatgggg
     2461 tgtatatccc caaagaggtg gccactttat ccgaccaaaa tgaaccagat aaagataaac
     2521 tttagcgtca tctatccagt cggaaccttt ttgacgcaac cgctctaagg cttcttttac
     2581 aaaattataa gttgcccagt ggtcagaatg aaggtcagaa ggatgaggca ggtaaataat
     2641 ctggggatgg aaaacctcta aaatgctctg aatatcctga attacttgct ctccataata
     2701 aggagcagaa agagtgtatc cggatagata aggcgattta tcagttttag tataaggaga
     2761 acgataggct tggtcatcgc ttcggtacat ccaccacatt ttttccattc cccggtctgg
     2821 ataacccaaa aaaatgacat tttccttttc cagacccaaa atggctgttg cctccagagc
     2881 ttctttttgc cggagatacc ccaattccac tcctcggggc aaatcttctg agagaccagt
     2941 catttcgtaa tcattcccga aactatcgcc agaagtcaca aaaactactt tgactatttt
     3001 cccctcactc agggatttct ggatagctcc cccgcaactc agggtttcat catcaggatg
     3061 aggagcaaag ataagaactc gctctgaatc atcaattaat ggttcaataa gcttggtata
     3121 aactttggaa taaacgtgaa aaagaggagc aaatatcgag ttaaaaggaa gaattttaat
     3181 tagaaagaag caaccgatca agattccaag cacagagagg gcaatttttg cccatcgccc
     3241 tctctggctt tttgctcttt tcctcataaa ttaccagtgt cagtgtttag catgggaaaa
     3301 ttataacact ttaaaaagtg agatatggca aattatttgt tagtatgcag cttaaaaaaa
     3361 tgagccgctc ttcaatctta caaatagccc tctacactgc tggctctttg agagcgtatc
     3421 cttccttcca gatttggcaa taaaatttat ctaatagggt aaaacttgct atgaagggat
     3481 acaatccaaa ggccgaaatc agaagctaac acagataggc taaaagcgtt attaaaacac
     3541 aaaaacatcc aaccaacaca aaaagcgcgg gaatttcggc tttttagatt atctgggatg
     3601 tgactcaatc tttcctgaaa ctatcagagg aggaaatagg ctcatgaaaa tgaatatcag
     3661 gtctaaactg accatcataa gcttgatacc acttgcactg tttatactgt taattttcgc
     3721 ttacataacg ccttctttga gagacgtagc cgagaatgtg atggaacaca gtttattaat
     3781 gaagctgcaa ggcgatatgc attcggtgca tttatttgcc gaaaaacact ttggagagat
     3841 aaaaatggtg gatggtgctc tggtggataa agacggcgta cctctcgaag gcagacacga
     3901 tatggtcgat gagataaccg ataaacttgg tgttgtagca accgtgtttg tgagagaagg
     3961 aaatgacttc agaagaatca ccaccagcat caagactgca gatggcaatc gggcagtggg
     4021 aaccatgctg ggcactgata gcgcagcata tgaaccagta atggcgaaaa atttgtatat
     4081 aggcaaagca gaaattttag gagcgaacta ttacactgcc tatgacccca tcgtggataa
     4141 gggggaagta gtgggaattc tctttatcgg tgtaccgact gacgaggcag aaagtatcgt
     4201 ctcaagcggg atatccggta cctcccggat gataatcatc ttctctgtag cagcactgat
     4261 tgcgagtatc attatcattt tcctcctggg tagctctatt tcccatcctg ttcaggtagc
     4321 cacaaaacaa gctgagcttc tgagagaagg aaacctctcg gtggaaattc cagcccacct
     4381 tctgaagcgc aaagacgagc tgggtctctt aggtagagct ttaaaaaaca tggctgagaa
     4441 atggcgggaa gtggtacaaa atgtcatcgg tgtttcttct aaactggctt cttcttccgg
     4501 agagctctct tcctcggtag aagaggtttc caaagccact caggagattg cgagaactat
     4561 gactcaggtg gcggagggct ccgttcgcca ggaagaagac ctacaacatt taagcggaac
     4621 tgtagaggga atcaacaaga tggctcaaaa catccgggag aaaacccagg aaaacgtgga
     4681 gcgcctcgac acagtgatga aagagcgact gaacgaaaac gccacagcac taaagcacat
     4741 tcaagaagaa attaaaaaag caactcaatc aggagctcaa gctggagaag aagcccaaaa
     4801 aggacaagaa cttcttggcc tcctcatgga gggaattact gccatctccc gggtaaccca
     4861 ggaagtagca gaaagtatcg ctgccttaga aggaagatct gaagaaatcg gcaaaatcgt
     4921 ggatgtaatt acggaaatca ccgaacagac caatctcctg gctttaaatg cagcgattga
     4981 agccgctcga gcaggagaag caggacgggg atttgccgta gtagcaaatg aggtgcgaga
     5041 tttagctgaa gattcagctc aggcagcgca gcagattgcc gggctcgtcg gagagattca
     5101 aagagatact caactggcag tcaaaaatat ggagagagct caagaagagg taaaagaagg
     5161 aaccggtaaa ggggaaaccg tagcccggag tttttcccag attcttaaga ccattcaggg
     5221 agttgtcgag aatatcaatc acattgcctc ttcttctcaa attcttgaga aagcacaaaa
     5281 agacctttta tctactcaag aagaaaccaa tatcgctacc agtgaagtgg ctaaagcagt
     5341 agaagaaatc agctattcca ttggagaagc aactgaacgg gtaaactcta tagttgccgt
     5401 agcagaagaa aatgctgctt cttcagaaga gatttccgct tctactgaag agcaaagcgc
     5461 atcgcttgag gagattacct cagctgtgga atccttagcc cacatggcgc aagaactcca
     5521 gataatggtg agccatttcc aggtatgaga gcccgacatg aaaaaatgaa aaaagccggt
     5581 tagctcaatt gaatcagcac cagcccatct cccatgaagg agtctccaga gtctcttggg
     5641 aaacctatgg ttcttatgga gaatgttttc atgccctcta attatcacca tctccgggag
     5701 ttaaccaaga tttgcgagaa gagaagcctc atgggctgaa aagatgaaag aattactact
     5761 gagaaagcta aagatagcag taaacatgct ctgaagccag agacaataag ttcaagttcc
     5821 tatcatcacc actacgagag gtagacccga acggggtaaa aagaaacagg gtaagaatat
     5881 acttattacc caaaaggcaa ccactatttc ccccttcatt tcggattctg agtcaagagt
     5941 ttccagctga ggtcatagtt ttataataag ccgctgtaaa gggattttat gcaagttagc
     6001 gaaaatattt gtttgacaaa agccaaacag ggagttataa attataaaat atggtattat
     6061 atacacgata taacatcata actggaagta aatggtgctt tttaaatttg ttttagaaaa
     6121 ggaggtgaca aaaagaatag caaatcgatt cagaggtttt ctcagtaagc ctgacttata
     6181 attaaaagaa agaggtgatt ttaagcatgt cgaaaagaat atccaagtta ctggtgataa
     6241 gtgttatttg cgtgttattt ttaagccttg gtagccaggt ttttgctcaa aaacttccgg
     6301 tggtaggaat ttctactggt tcgtcaggaa cttcttggcg agacatcatg atagctgcta
     6361 tgcaggaagt cggcgatgag tacaaggctg caggcaaaat tgctgattac aagatagtaa
     6421 ataatattgt taacggtgac gccacagagc aggctaacat ccttcgcgat tttatatccc
     6481 agggaatgga tataattatg gtgaacccca actctcccga tgcattaaat ggagtaataa
     6541 aagaagccca ggaagaaggt attctggttg tttcttttga tgccacggta accgctcctg
     6601 atgttgtcaa cataaccctg gaccattatg cctggaataa gaaaaatgtg gaattcattg
     6661 cctccactct ccagaaaggg aacgccgttc aaatctatgg tttagaagga catccggcaa
     6721 acaatgaacg aattagagca acctatgagg tgcttgcgaa ctaccctgat atcaagttga
     6781 ttgctgatac ctcaggatac tgggaccaga ccaaggctaa agaagtagcc acccagatca
     6841 tcgcttctgg ccaacaaatt gacgcggtaa tcactcaaga tggtatggca tttggaattc
     6901 tttctgcctt cctagatgcc aataagctcc ctaaagttat gtatggagac ccaggcactg
     6961 ctttctttaa agaatggaaa aaattaagag accagggagc cgatttcaag gcttgcactc
     7021 agcccaatcc tcccggaatt ggtggaactg cttttcgcat agctctgcag ctttataacg
     7081 ggaaaaattt caagccggac atcttgaaag ataatacttt ctattatcag gttagcatgt
     7141 tcgtcaccga tgaaaacttt gatgaggcgt gggaactcct gaaagacaaa ccagacgact
     7201 atctgttaag cgaaattatg tctgaagagg aagtagcagc actatttgaa taaacgagac
     7261 aaatttactc ctaattcgaa ggggtaaagt gagcttctat cccttcgaat taggagtaaa
     7321 gtaaggaatc tgttcaactt tgtatttact atggtgagag ctaaccggta tgaataggag
     7381 accaaagaat attaaaatgc agagagactg ccctgccagc ccgaggaaaa ggcacaagtt
     7441 ccaatgcttt ttctctactt tttttctaaa atgccaggtc ctctggcgtg ccacacccca
     7501 ggtggaatcc acgtgaaaaa aaagagacta tcctctcaag caggggaaac aaaaaacgta
     7561 gagaatggct aattttggag agtgagggat aaagtgtgat agtcttagaa gcgataggaa
     7621 ttaaaaagaa ttttggagga gtagttgctc tatccaatgc caatttgcgc tgttaccagg
     7681 ggagaatcac cggtcttctg ggagccaatg gttcgggtaa aagcactatg tccaaaatta
     7741 tctgcggtgt ttatcaagcc gatgggggag agataaaata taaaggtaga acggtgagat
     7801 accggagtcc gagcgaagct cgaaaagatg gaattgccat ggtgtttcaa aatctcagtc
     7861 tggtggcaga tttgactgtc tggcaaaata tcgtcctggg aaaagaagaa agcaatggta
     7921 tatttttaaa caatgatagt gccaaagaat tatccagaaa gatattggct caacttcttc
     7981 ctgacctaga catcaacaaa aaagtttccc aattaaatcc aggagaaatg cagattgtag
     8041 aaatagccaa ggctatttct caaaatcctc aactccttat cctagatgag cccaccgctg
     8101 ccttagagca agcccaggtt aaaaatcttt ttgactgcat gagagctctc gctaaggaag
     8161 gagtagctat gatttttact tcccatcgcc tctgggaagt aatggaaatt tgcgacgatg
     8221 tcaccatttt cagaaatggt gaaaatgtag ccactattga ttttgaaaaa gatgaaaaag
     8281 atccggaaaa aattataagc tatattactg gagatactca aaaaacaaaa gtggagaaga
     8341 agcacgtggc ggtaactgat gaaacaattt tgagtataaa aaatttaaat tatggcaagt
     8401 ccctgaaaaa tgtgtcattc gacctgaaaa aaggagaaat actgggcata ggagggcttg
     8461 caggacaagg acagcttgag ttgatgcacg ctttagctgg tgctttccat agtcttcatt
     8521 gcgacgctga gctaagagga aagaaaatca aactaaatag accattaaat gccataaaag
     8581 agggaatagt gtttgtcccc ggagataaac aattggaagg tctttttttg aagcgctcgg
     8641 tattatttaa tatgattttt cctaaaatgg gtcttcctgg tcatccttct tttatccctt
     8701 atgatgaata tcggagggaa tgcgaggaga tagtggaggt cctttctata aaaacgccca
     8761 ctgttgatgt accagtagag aacttatcag gcggaaatca acagaaagtc gtggtgggaa
     8821 agtggttgcc ctttgacatc aatgttcttt tgctggctga tccagcaaag gggattgatg
     8881 tcggagcaaa aagagacttg tatcagtata tcgtgaatca agtgcaagaa aagaatacga
     8941 gtgtcattct ttatgccagc gataatgaag agcttgtaga atactgtgac agggttctca
     9001 tcatgtacga agggcagatc gttgcaagtt tagaaggcgg agatataaac caagacacaa
     9061 ttatcgcctg ttctatgcga ttgagataag gtgtggtgaa taatgaataa aaacaatcaa
     9121 aattcctcaa gacctgcttt atcaaattat gtttttcgtc ctgaattttc cagcttatta
     9181 atattgatag tcatgtttac cattactgcc attttacagg aaaatttctt tacccttaaa
     9241 tccatagtca gaaacataaa cgcctttgct ccattgatac ttctagcgat gggcgctgca
     9301 gtagtcatta tatctggtgg aatagacctt tctgctgggt cggcactttc tctttttacc
     9361 tgcgtgctaa cgacagttat gaagaaatct gatcctataa ctggagtcta tgctatcctt
     9421 gtcactcttg ccgtagcaat tttagtgggt gtagttaatg gctttggtat tggctacttg
     9481 agaattcccc ccgttattgt tacctttgct acctcttaca tatggttggg aattggcctt
     9541 ttcttgcgac ccaccccggg gggagaatcg gttgagtggt tcaggatatt ctataacccg
     9601 ggaatgatag aaggaattca tccctatatt aagcaatttg gccgtgttat ccctcctgct
     9661 ttgattttaa tcttagcggc ctgtcttata tggtttatcg ttagcaaaac caggatagga
     9721 agacatatct acgcggtggg aagcaatagc gagagcgctt atgccagtgg tattaatacg
     9781 gcaaaggtgc aaatgcaagc ttgcattatc aattcgatat ttatcttttt agctgctctg
     9841 ttttttgtag gacaaaatca gtccggagac gcaagaatgg gtgaccccct gaccttgagg
     9901 gcaatagcct cagcaattgt gggaggtgta gccatcagcg gaggaaaagg aagcgtttat
     9961 ttcgccctgg taggagctct aattcttaat tttgtgaaca aaattatctt ctttgctgat
    10021 atttctaccg cctaccagac tctggtgtca ggattaatcg tgattattgc cattgctgct
    10081 cccgtagcct ataccctgta tagtaaagaa taatttaaag gagaataacg gtatgaatct
    10141 gggtatatca atgttttttg aaagatataa aaaaataatc gtggctttta tcttgattgt
    10201 gctccttttt gcattgggtg gaataatagt gcccaacttc ttgaccgtag aacaagtttt
    10261 gtttacctta aagctggctt cattcaccgc cctgtttgcc ctgtgtcaaa tgattgtaat
    10321 tgctgctgga ggtagtggtt tagacttatc tgtggggtat gctgctactc ttactgctgt
    10381 tctcaccgcc agcgttatgg atggccaaaa tgccaatctg ggagctgcta ttttaattgc
    10441 tctggctctg ggaagtgcca taggtttagc caacggagcg ctggtttctt tcctggagct
    10501 tccacctctc gtcgtaacca tggctatggc taacattctc cagggcatcg taaacgctta
    10561 tacagcagga agaaatatta ccgggagacc ttcaccgatt ctggcaactc tcgctgccaa
    10621 atccaccggt aaatttccta acatcctttt tatcttattt actatcactg ttttggttat
    10681 gattgtgctt aataaaacaa agtggggtac gattcttttt ggagtagggt ctaatgaaac
    10741 aactgcatac ctgagcggta ttaacgtgaa agcagtaagg tgcgtcgttt ttgtaattag
    10801 tggaatactg gcaagcctca cagggctgct attgattggc aacatgggaa ttgcttttaa
    10861 ggatatggcc tccaactacg ttatgcccag tattgctgcg gtagtggtag gtggagtgtc
    10921 gctcaccggc ggagatggaa attacatcgg agttattttg ggagctatct ttttacaggc
    10981 tttaaccaac ttattgatag catttggtct gggagatgct ggaaaatggt tggcattcgg
    11041 tatcgtgctt tatgttctcc tttcctttta tgccagcgac cgtcgtagcc gataaaatag
    11101 aactataagg gggtaacacc ccctatgacc acgcaagaaa tcaaaagcaa aatataaaac
    11161 aacaaagact accgagggag aaaagtatcc ctcagtgggg agtgctcctt tgtgaacccg
    11221 actatagggg gaaaccaatc cccttataac ccccagaaaa agtgaaaaaa acaaagacaa
    11281 ctggaagaaa agatttccca gcaagatagg agcgttttcc cccttacgaa cccctttatc
    11341 ccgactttga cacttcactt agctgaaacc cgcatcaaat ctatattctt taaattagaa
    11401 tatgctattt tgccactaca ctcactacct taagtggtct acagagatag ttcttcttta
    11461 ggggtgatat ttttaggggg tttaattttg aagagtttta agattttatt actcagttcc
    11521 gttcctttaa ttttgagtaa gaactgttga ttttcaattt ccacttcagc aaaattcagg
    11581 gagtttaaga cttcccgaat ttcctttgga gaagctttct ctttcccgc
//
LOCUS       DAOPSS010000045        11463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4744_length_11463_cov_8.613867, whole genome shotgun sequence.
ACCESSION   DAOPSS010000045 DAOPSS010000000
VERSION     DAOPSS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11463
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4744_length_11463_cov_8.613867"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..449
                     /locus_tag="PKN31_04075"
     CDS             66..449
                     /locus_tag="PKN31_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99135.1"
                     /translation="MDARLNFRHDKKSGTLQRIKTRLPRQAKNSKANSQREARKPQEG
                     YNLRRDLQSFGGFKREQKKPSVPTNLSLFFLAFSEKFQDGLPLKSFELLIFQTEALFS
                     ILRLNSSEPMAIIIYHHFSISSIGS"
     gene            835..1368
                     /locus_tag="PKN31_04080"
     CDS             835..1368
                     /locus_tag="PKN31_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOA99136.1"
                     /translation="MKSQEITKISLLVATGVVLPSLFHVSGIPGAVFLPMHLPALLGG
                     FFLSSGSAAFLLGFILPLLNFFLTGMPPFPGFLVMMGELGGYGILVFFLYRKKRWGVF
                     PSLFASLLGGRLISILGNWILIALVLGKKFSFLPVLNTLFVISLPGVAIQMVLVPLIV
                     KGVENFVQGREASPGSF"
     gene            1331..1927
                     /locus_tag="PKN31_04085"
     CDS             1331..1927
                     /locus_tag="PKN31_04085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA99137.1"
                     /translation="MCREEKQVREAFNRLAGEWDERALSSPDKIRYLLSFLTIPPSST
                     VLDVGSGTGILIPFLREKMGSSGLIVELDVAEEMLEVAKRKFGEEGIEYHQGSAERYK
                     DSRRYDLVIVYSSFPHFWDRDLALQNLISLLIEGGELSIAHSESRQTINQRHRGDGAP
                     PAFTLPPGEELAWELKKRGLEITRVIDNQELYLVTGRK"
     gene            complement(1939..2340)
                     /locus_tag="PKN31_04090"
     CDS             complement(1939..2340)
                     /locus_tag="PKN31_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOA99138.1"
                     /translation="MNQVEKIFCPVCQVSFILREEKEAGKKITCPVCGAVLVLEKDNS
                     SWLARRPDDLTPEEEIRARIENFARLRNYKFNELKEPLVEGLLKKHERYGDFYCPCKI
                     DNIPENVCPCLETRQGSVERNGRCHCGLFWK"
     gene            complement(2337..2585)
                     /locus_tag="PKN31_04095"
     CDS             complement(2337..2585)
                     /locus_tag="PKN31_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOA99139.1"
                     /translation="MKKVMLYALSTCPFCQMTKKYFEKHNIPYDFVDVDLLEDEEKEK
                     TVAKVQEISGRRAFPVIVIGDKVIVGYDELSISEALKE"
     gene            2728..3531
                     /locus_tag="PKN31_04100"
     CDS             2728..3531
                     /locus_tag="PKN31_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA99140.1"
                     /translation="MFDIWAVSTNTYHGFSLEEALTGIAKAGFPYVELACVEGWTNHF
                     SVINHREEDLKKLRNALSRLGLQVASISAHSDLSTPEGVSYLEKALDLARVLGARVVN
                     TGTIEEGSKKGILISNLQVLASEAAKRGVKIGLEIHGDFLKDGRAACELMEELNHPAV
                     GINYDTGNVIFYGGVRPEEDVKNCLPWIVHFHLKDKRGGYKVWDFPPLGEGDIDFGKI
                     FALAREENIEVPWSVEIEFDGKFQHSREFVDQAVKKSFDYLQSLIGEEK"
     gene            3528..4400
                     /locus_tag="PKN31_04105"
     CDS             3528..4400
                     /locus_tag="PKN31_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOA99141.1"
                     /translation="MMEREDFTFYDLRRREKSKSIELLFPGWVEGEERVAIFCPHDDD
                     GILGPGYLLQAIPLFGGEVHVVIFCNGSGGYSVIEHKNIITALRARETVLAYAHLGVK
                     EERIHRLDYDDYSVWPFLGWKLPGGEEGTFRKILPLLRRLKITRVLVPNGYREHLDHY
                     ATFLVGAFDTPQVGDPVMADWGIAKGIKSVLQYAVWSDFSPEDTLLEKDALNVRANRA
                     LCAPREAEEKINLAMSEFKTQAQIIAGLLEQRKERNMEKGMIEVYLSFDPRPRCEYDK
                     YVRRIQEIESKEEK"
     gene            4397..5407
                     /locus_tag="PKN31_04110"
     CDS             4397..5407
                     /locus_tag="PKN31_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA99142.1"
                     /translation="MMKKIALLGAGFIAGVHMEGWKRVEGAEVCAFFETNLEKASAFQ
                     DKYQIPHYNSFSRLLEEEKVDIVDICLPTFLHREYTEMAASHEKHVFCEKPIALTVED
                     AEAMKRICERSGVKLMVGQVLRFWGEYVKARELVEEGAVGKVLSVDAFRLSLPPTWSV
                     DSWILKPELSGGAAVDLHIHDLDYVNWLLGEPQVVFAQGVRSPQNSWDHMVTVVNYGG
                     VIAHIEGGWMMRGDFPFTCGFRILGDEGVLEWSSRAGVNIEEREKTSPLVLYRNGEAK
                     KEMEVREEDPYYLELEYFLDCIENNKPVERGTPEQAILALRLALAARQSAEEGEKVKL
                     GE"
     gene            5407..6681
                     /locus_tag="PKN31_04115"
     CDS             5407..6681
                     /locus_tag="PKN31_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA99143.1"
                     /translation="MRKILLVEKSALWREKIGNWLREEGYYLISVRDRDLAINCLEKE
                     EIDIVLVEEKVLRENSFSLLSFIRQSFPDMIIIALGESNDSFPLQELFSKGVYDYISV
                     SSAPSRILSVIKRAEEKVSLLEENKDLKKRVISRPSFAGIIGVSEKMQRVFSLILQVS
                     QVQRPILLVGEQGTGKELAARAIHQYAFSEEKPFLKVLCVALPLEILPQEKPKEGTIY
                     FEDVHLLPYKQQAEMLAFLEEYRFSKKSSDLRIIASSEVPLEGKVAEGSFRNDLYYFL
                     NAVKIEIPPLRERKEDIPFLVDRFLQEIEEETGREGIRVDKEALRFLIEYDWPGNVVE
                     LRNTLEGMVILSSGDLLTSEDIPDYIKKRQREELLGVKVGMSMEEVEKILIQETLKFT
                     RFNKTRAANILGISLRTLYRKIEQYDLALERN"
     gene            6860..7150
                     /gene="groES"
                     /locus_tag="PKN31_04120"
     CDS             6860..7150
                     /gene="groES"
                     /locus_tag="PKN31_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOA99144.1"
                     /translation="MKIKPLADRLLVKRMEEEEVKKGGIIIPDTAKEAPQQGKVIAVG
                     TGRMGEDGKRIPLEVKEGDRVLFGKYAGTEVEIEDEEYLIMREDDVLGIIEG"
     gene            7167..8783
                     /gene="groL"
                     /locus_tag="PKN31_04125"
     CDS             7167..8783
                     /gene="groL"
                     /locus_tag="PKN31_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888904.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOA99145.1"
                     /translation="MMAKQILFEEEARQKVLGGVKTLADAVKVTLGPRGRNVIIERKF
                     GSPVITKDGVMVAREIDLEDPNENVGAQLVKEVASKTSDIAGDGTTTATVLAEYIYRD
                     GLRAVVAGSNPTLLKRGIDKAVEAVIGKLKEISKEVSGKKEVAQVATIAANNDESIGN
                     IIADAMDKVGKDGVITVEEATGLETTLEVVEGLQFDRGYLSPYFVTDPERMEAVLEDA
                     YILIYEEKISAVKDLLPVLEKVVQRGKPLLIIAEEVEGEALATLVVNKLKGVINCVAV
                     KAPGFGDRRKEMLQDIAVVTGGTFISSELGIKLENITLEDLGQARKIVVDKENTTIVE
                     GAGKKEDIMARMNQIKKQIEETTSDYDREKLQERLAKISGGVAVIRVGAATETEMKEK
                     KARVEDALNATRAAVEEGIVPGGGVALIRCESAIDGLNLDGDEKIGALVVKRALSEPA
                     KMIAQNAGEEGSVIVEKIKAADNVNLGYNALTGKFVDMIEDGVIDPAKVVRVALQNAS
                     SVAGVMLTTELIVTEIPEKEPAPVPPMPEY"
     gene            8876..9139
                     /locus_tag="PKN31_04130"
     CDS             8876..9139
                     /locus_tag="PKN31_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOA99146.1"
                     /translation="MPIYEYQCTQCGKTFEELQSFSDKPISQCPFCKGRVKRLIGRNV
                     GLVFKGSGFYCTDYRSPSSSSSSSCSTCSSDDCSTCNNGGSGL"
     gene            9148..9777
                     /locus_tag="PKN31_04135"
     CDS             9148..9777
                     /locus_tag="PKN31_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOA99147.1"
                     /translation="MDTWGYLLIAGLLVLFAFLWGARRKGSSVSVSEFWQEKEKEFGE
                     NKILSGFSRYLGGHPRFEAVSDGLLFLMSKSLWFENFEKGPNIFGITPPFEKVIFRIL
                     LSQIEEVESLPEKELLKRGFESQLWRYRRLSPRPFYLAVKYKDEWERERTLYFDSMVD
                     ADTWKQELERAKSNYIPEEEIKESGVCPQCGKKIDPDFKLCPYCGCRLS"
     gene            9787..10674
                     /locus_tag="PKN31_04140"
     CDS             9787..10674
                     /locus_tag="PKN31_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOA99148.1"
                     /translation="MSFQAGHYQDFSKATGCTVFLFEQPNRAVAAVRGGAPGGRETAT
                     LFPGNLVEGVDAIFFSGGSALGLSCGEGVVDFLRERKKGFKTPGGTVPIVAGAVIYDL
                     EVGEPAFPDREWGYNAALSSEKVVKEGTIGAGTGATVGKLMGMEGAVKGGFGKGRAKV
                     EGEEISAFVVTNSWGNIYHPRTGELVAGVKMKGRGTTFSPFNTTLTLVVFDFFLSREE
                     LFSLVGVVHSALASCIRPFATLYDGDTIFLVSTGKKKVSNYLSLISGIYKSVTEGVLN
                     SVTKATGLAGILAKNDIIV"
     gene            10771..11271
                     /locus_tag="PKN31_04145"
     CDS             10771..11271
                     /locus_tag="PKN31_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOA99149.1"
                     /translation="MEKRTKKFVLAGVLGAVSVILSLTPLGLIPVPNLASYATTMHIP
                     AIIGGIIGGPEVGALVGLILAFSTLHLFAGNVLACVLPRLLIGVVAYYIWKWSGKKDW
                     GVVLGSLGGTFINTIGVLGLMVVTGFFTWAQVLPVFVLNGSLELVISGIIVLPLVRIL
                     RRVVKF"
     gene            11268..>11463
                     /locus_tag="PKN31_04150"
     CDS             11268..>11463
                     /locus_tag="PKN31_04150"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOA99150.1"
                     /translation="MSRILVIDVGNTHTTWGVYEDKALLSNWRVSTSPYRTSDEWSFL
                     LQNLLEGEGIKRATLDSVVVS"
BASE COUNT         3190 a         2024 c         2935 g         3314 t
ORIGIN      
        1 ggctctaaat aatgggaggg gacacgccct atcgttccca atggaaggaa caaaaaagca
       61 aaaacatgga tgcccgccta aatttcaggc atgacaaaaa aagtggaacc ctccagagga
      121 taaaaacgag attgccccgc caagccaaga acagcaaggc aaattcacaa agagaggcaa
      181 gaaaaccaca ggaaggctac aacctgagaa gggatttaca gagtttcggg gggtttaaga
      241 gggaacaaaa aaagccatcg gttccgacaa accttagcct tttctttctc gcatttagcg
      301 agaaatttca ggatggactt ccactcaaaa gctttgagct tcttatcttt caaacagaag
      361 ctcttttttc catcctccgg cttaactcct cagaaccgat ggcaataata atataccacc
      421 acttttccat ttcgtcaata ggtagttaaa aaattttttg aaaattattt tctttttata
      481 ggttgcgaga taccaagcta ttacgccctc ctcatttttt tcacagttat cgctcgctgg
      541 cataatttct tcatactctg ctttaaagtc ttctccggga gcactaattg ctttttagtc
      601 atacttaaat cagtggctct cgagtgcgct gtagggtgaa ggcaagtgac gtaaatcatg
      661 aggagttacc ttatagccgc ttttgcgaga gtatttgtta atccttttat caaaagaagt
      721 ccagagagta cctttctctc aaaaccacaa aagcgattcc actcgagagc ctcagtttcc
      781 ttgacagatt gttattcgat gttacaattt tttagtggag cgattattga atagatgaag
      841 tctcaggaaa taaccaaaat ttctctatta gttgccacag gagtcgtttt accgtctctt
      901 ttccatgttt ctggcattcc cggagcggtt ttcttgccta tgcatttacc cgctcttttg
      961 ggaggttttt tcctctcatc agggagtgcc gcttttttac tgggctttat tcttcccctt
     1021 ctcaattttt ttctcactgg tatgcctcct tttcctggct ttttggttat gatgggagag
     1081 ctgggaggat atggaatttt ggtcttcttt ttatacagga aaaagagatg gggtgttttc
     1141 ccttctcttt ttgcctctct tttggggggg agattaattt ccattctggg taactggatt
     1201 ttaattgcct tagttctggg taaaaaattt tcttttcttc cggttttgaa taccttgttt
     1261 gtgatttccc ttccgggagt ggccattcag atggttttag ttcctcttat agtgaaagga
     1321 gtggaaaatt ttgtgcaggg aagagaagca agtccgggaa gcttttaacc gtttggccgg
     1381 ggagtgggat gagcgtgctt tatcttctcc ggacaaaatc cgctatcttc tgtcctttct
     1441 cactattcct ccttcttcta ccgttttaga tgtaggttca gggacaggga tattaattcc
     1501 tttcttgcga gaaaaaatgg gttcttctgg cttaattgtg gagctagatg tagctgaaga
     1561 aatgttagaa gtggcgaaaa gaaagtttgg agaggagggc attgaatatc atcaggggag
     1621 tgcggaaaga tataaagaca gccgcagata tgacctggtt attgtttatt cttcttttcc
     1681 tcatttttgg gatcgggatt tagctctcca aaatttaatt tctcttctca tagagggagg
     1741 agagctatcc attgcccact ctgaaagccg gcaaaccatt aatcaacggc accgaggaga
     1801 tggagctcct ccggctttta cccttcctcc gggagaagaa ctggcttggg aattaaaaaa
     1861 acggggcctg gagataacga gagtcattga taaccaggag ctctatcttg taacagggag
     1921 gaaatagggg ggaaccccct atttccagaa taatccgcaa tggcagcgtc cattgcgttc
     1981 cacgctgccc tgtcttgttt ccaggcaagg acatacgttt tcgggaatat tatctatttt
     2041 gcacggacag taaaaatctc catagcgttc atgttttttc agcagtcctt ccaccagagg
     2101 ttctttgagc tcattgaatt tatagttgcg cagccgggca aagttttcta tccttgccct
     2161 tatttcttct tctggagtta agtcgtcagg tcttctggct aaccagctac tgttatcttt
     2221 ttctaaaact aaaactgcac cgcacaccgg acaggttatt tttttccctg cttctttttc
     2281 ttctcgcaga ataaaactca cctgacacac cggacagaat attttttcta cctggttcat
     2341 tctttgagag cctccgagat gctcagttcg tcatatccca ctattacttt atctcctata
     2401 actattaccg ggaaagctct tcttcccgat atttcttgta ctttagccac tgttttttct
     2461 ttttcctcat cttctaagag gtctacatct acaaagtcat aggggatgtt gtgcttttcg
     2521 aagtattttt tagtcatctg acaaaaaggg caggtgctca aggcatagag cataactttt
     2581 ttcatatctt tcactccttt ttaggaaagt tataattcaa accgaaaatt aaagaattta
     2641 ttctttatct atttcccctt gaactattct atctcttttc agaaaaagag aaagatgata
     2701 ctattttatt aaaaaagggg gattaatatg tttgatatct gggcggtgtc taccaatact
     2761 taccatggtt tctctctgga agaagctctg accggtatcg caaaagctgg ctttccctat
     2821 gtagaattag cctgtgtgga aggctggacc aatcacttta gtgtaattaa tcaccgggaa
     2881 gaggatttaa agaagctacg taatgcttta tcccgcctgg ggcttcaggt ggcaagcatc
     2941 agtgcccatt cagacctatc tacccccgag ggagtttctt atttagagaa agctttagac
     3001 cttgctcggg tcctaggggc ccgggtggtt aacactggaa ccatagaaga aggaagcaaa
     3061 aaggggattt tgatttccaa tttgcaagtt ttagcttccg aggcagcaaa aagaggggta
     3121 aaaattggcc tggagattca cggagatttt ttgaaagatg gacgggcggc ttgtgaattg
     3181 atggaggagt taaatcatcc agcagtgggg attaattatg acacagggaa tgtcatattt
     3241 tatgggggtg tccgtccgga agaggatgtt aaaaattgtc ttccctggat tgtccatttt
     3301 cacctcaaag ataaaagggg agggtacaag gtatgggatt ttcctcctct gggagaagga
     3361 gatatagatt ttggtaaaat atttgccctg gcaagagaag agaatataga agttccctgg
     3421 agtgtagaga ttgagtttga tggtaagttt cagcattccc gggagtttgt ggatcaggca
     3481 gtgaagaaat cttttgatta tctgcagagt ttgatagggg aggagaaatg atggagagag
     3541 aagattttac tttttatgat ttgcgccgga gagaaaaaag taaaagtatc gagcttctgt
     3601 ttcccggctg ggtagaagga gaggagcgag tagctatttt ttgccctcat gatgatgatg
     3661 gtattttagg gccggggtat cttttgcagg ccattcctct ttttggggga gaagtgcacg
     3721 ttgtaatttt ctgtaatggt tcgggaggct acagtgtcat tgaacataaa aatatcatca
     3781 ctgctctccg ggctcgggaa accgttttag cttatgctca tctgggagtt aaagaggaaa
     3841 ggattcatcg tttagattac gatgattata gcgtctggcc ttttttgggc tggaagttac
     3901 cgggaggtga ggagggaact ttccggaaga ttttacctct tctccgccgg ctaaaaatca
     3961 cccgggtttt agtgcctaac ggctatcggg aacacttaga ccattatgcc acatttctcg
     4021 tgggagcttt tgacactcct caggtgggag atccagtaat ggctgattgg gggatagcca
     4081 aaggaataaa atcggttctc cagtatgcgg tgtggagcga tttttctcct gaagatacct
     4141 tgctggaaaa agacgctctg aatgtcagag ccaaccgggc gctgtgtgct ccccgggagg
     4201 cagaagagaa gataaatctg gctatgagtg aatttaaaac tcaggctcaa atcatagccg
     4261 ggcttctgga gcagagaaaa gaaaggaata tggagaaagg aatgatagag gtttacctct
     4321 cttttgatcc tcgcccccgt tgtgagtatg ataaatatgt aaggagaatc caggaaatag
     4381 aatctaagga ggagaaatga tgaaaaaaat tgctctttta ggagcaggtt ttattgctgg
     4441 ggtgcatatg gaagggtgga aaagggtaga aggagcagaa gtctgtgctt tttttgagac
     4501 aaatttagag aaagcttccg cttttcaaga taaatatcag attccccact ataattcttt
     4561 ttcccgcctt ttagaggaag aaaaagtaga catagtggat atctgcctgc ccactttttt
     4621 gcatcgagaa tatacagaga tggctgcttc tcacgagaag catgtttttt gtgagaagcc
     4681 catagctttg actgtggaag atgctgaggc tatgaagaga atttgtgaga gaagtggagt
     4741 aaagcttatg gtagggcaag ttcttcgttt ttggggagaa tatgtaaaag cccgggagtt
     4801 agtagaagaa ggagcggtgg gtaaagtttt atcggtggat gcctttcgtc tttctcttcc
     4861 tcccacctgg tcagtggata gctggatact gaaaccggag ttgagcggag gagcagctgt
     4921 ggatttgcat attcacgatt tagattatgt aaactggctg ttaggagaac ctcaagttgt
     4981 ttttgctcag ggagtgcgct ctcctcagaa ttcctgggac cacatggtta ctgtggtgaa
     5041 ctatggtgga gtgatagctc acattgaagg aggatggatg atgaggggcg attttccttt
     5101 cacctgcggc tttcgtatcc tgggtgatga gggagtttta gagtggtctt cccgcgccgg
     5161 ggtgaatatt gaagaaagag agaaaaccag tcccctggtg ctctatcgaa acggggaagc
     5221 aaaaaaagaa atggaagtga gggaagaaga cccctattat ctggagctgg agtattttct
     5281 ggattgcata gagaacaata agcctgtgga gagggggacc ccggagcagg ctattctggc
     5341 tttgcgattg gccttagctg cccggcaatc agcagaagag ggagagaagg tgaagcttgg
     5401 agagtaatga ggaaaatact tttagtagaa aaaagcgctt tatggcgaga gaaaataggc
     5461 aactggttgc gagaagaggg ctattatctt atttccgtta gagatcgaga cctggctatc
     5521 aactgcctgg agaaggaaga aatagatata gtattggtag aggaaaaagt tttgcgggaa
     5581 aatagttttt ctctgctctc ttttatccgt caatctttcc ccgatatgat aataattgct
     5641 ttaggagaga gcaatgattc tttccctctc caggagctgt tttctaaagg cgtgtatgat
     5701 tatatttctg tttcttctgc tccttctcgt attctctcgg taataaaaag agcggaggag
     5761 aaagtttccc ttttagaaga aaacaaagat ttaaagaaaa gagtgattag ccgcccttct
     5821 tttgctggta tcatcggggt atcagagaaa atgcagcgag ttttcagttt aattcttcag
     5881 gtatcccagg tgcaaaggcc cattcttctc gtgggagaac aaggaacagg taaagagctg
     5941 gcagctcggg ctattcacca gtatgctttt tcagaggaaa agccttttct caaagttctc
     6001 tgtgttgctc tgcctctgga aattttacct caggagaaac caaaagaagg aaccatttac
     6061 tttgaagatg tgcatctttt accttacaag caacaagctg aaatgttagc atttttggaa
     6121 gaatatcggt tcagtaaaaa gtcatcagat cttcgaataa ttgcttcttc tgaagttcct
     6181 ctggagggga aagtggctga agggtctttt cgcaatgacc tctattattt cctcaacgcc
     6241 gttaaaatcg agattcctcc ccttagagaa agaaaagaag acattccctt tttggtggat
     6301 cgttttttgc aagaaattga agaggagacg ggaagagaag gaatcagggt ggataaggaa
     6361 gctctccgtt ttctcataga atacgattgg ccgggaaatg tcgttgagtt gcgcaatacc
     6421 ctggagggta tggttattct ttcttctggg gatttgctta cttctgagga catacccgat
     6481 tatattaaaa agagacaacg agaagagttg ttaggagtaa aagtggggat gagcatggaa
     6541 gaggtagaaa aaatcctcat tcaagaaact ttgaagttta ctcgtttcaa taaaaccaga
     6601 gctgccaata ttttagggat aagcttgcgc actctttatc gcaaaataga acaatatgat
     6661 ttagctctgg agcgtaacta aatactttga cattttgtca aggtattttt ttatttttgt
     6721 caaaaattca aatactataa caattctttt gtcattttgg cagaaaattt accttgattt
     6781 accttgccat actcttgcaa aaattgtttt ggggatgtaa aatagacgat ggctaatttg
     6841 atgaaagggg tgatagtgaa tgaagatcaa accattagca gaccgtttgt tggttaaaag
     6901 aatggaagaa gaagaagtaa aaaaaggagg cattataatt cctgatacgg caaaagaggc
     6961 gcctcagcag ggaaaagtga tagcagtagg tacaggcagg atgggcgaag atggaaagcg
     7021 cattcccctg gaagtgaaag agggcgatag agttctcttt ggtaaatatg ccggtacaga
     7081 ggtggaaatc gaagacgaag aatacctcat aatgcgcgag gatgacgttt tgggtataat
     7141 cgaaggttaa aagaagggag gtatgattga tggccaaaca aattctattt gaagaagaag
     7201 cacgacagaa agttttaggg ggagtgaaaa ccctggctga tgccgtgaaa gttaccctgg
     7261 gaccccgggg aagaaacgta attatagaga gaaaattcgg ctctcctgtt atcaccaaag
     7321 acggggttat ggtggctaga gaaatcgacc tggaagaccc caacgaaaac gtgggagctc
     7381 aactggttaa agaagtggct tctaaaacca gcgatatagc tggtgatggg actaccactg
     7441 ctacagtttt agctgaatac atatatcgag atgggttgcg ggcagtggtg gctggttcta
     7501 accctacgct tcttaaacga ggcattgata aggcagtaga agcggtaatc ggaaagctaa
     7561 aggaaataag caaggaagta agtggtaaaa aagaagttgc tcaagtagca actattgctg
     7621 ctaacaacga tgaatctatt ggaaacatca ttgctgatgc tatggacaaa gtgggtaaag
     7681 acggagtcat taccgttgaa gaggcgacag gattagagac tacattagag gtggtggaag
     7741 gactgcagtt tgatcggggc tacctttctc cttattttgt aactgacccc gaaagaatgg
     7801 aagcggttct tgaggatgcc tatattttaa tctatgaaga gaagatatca gcggtgaaag
     7861 acttgctccc ggttttagaa aaagtggtgc agagagggaa gcctctgttg attattgctg
     7921 aagaggtgga aggagaagct ctggctactt tggtggtcaa caagctaaag ggtgttatta
     7981 actgcgtggc agtgaaagct cctggctttg gtgaccgacg aaaagagatg cttcaggata
     8041 tagctgtagt tactggagga actttcattt ccagcgagtt aggaataaaa ttagaaaata
     8101 taactcttga agacctgggt caggcaagaa aaatagtagt agataaagag aataccacta
     8161 tcgtagaagg agccggtaaa aaagaagata ttatggctcg tatgaaccag attaagaagc
     8221 agattgaaga gaccacttct gattatgacc gggaaaaact gcaggagcga ttagctaaaa
     8281 tttctggtgg tgtcgcggtg attcgggtag gtgcagctac cgaaacagag atgaaggaaa
     8341 agaaagcccg ggtggaagat gctctgaatg ccacgagagc ggcagtagaa gaaggaatag
     8401 tcccgggagg cggagtggct ctgattcgct gtgagtccgc cattgatggg ctgaacttag
     8461 atggcgatga gaaaataggt gctctggtag taaagagggc cctgagtgaa ccggcgaaga
     8521 tgattgctca gaatgctggt gaagaaggct cggtaatagt agaaaaaatt aaggctgctg
     8581 ataatgttaa ccttggctat aatgctctca ccgggaagtt tgtggatatg atagaagatg
     8641 gagtaattga cccggcaaaa gtagtacgag ttgctctgca gaacgcatcc agtgtagctg
     8701 gagtaatgct tactacagag ttaatagtta ctgaaattcc agaaaaggag cctgctccag
     8761 ttccaccaat gcctgagtat tgataggtga ggtataataa taaaagtgga cgaagaaaaa
     8821 ccacccggta aaggatatcc tttaccgggt ggtttaccat atggaggtga aagcgatgcc
     8881 gatttatgaa taccaatgca ctcagtgcgg taaaactttt gaggaacttc aatcttttag
     8941 tgacaagcca atatctcagt gccctttctg caagggtaga gtaaaaagac tgatagggag
     9001 aaacgtgggc ctggtattta agggaagcgg tttctattgc accgactacc gttctcccag
     9061 ctcttctagt tcttccagtt gcagtacttg ctccagcgat gactgttcta cctgtaataa
     9121 tgggggaagt ggcttgtaat aaaagagatg gacacctggg gatatctttt aattgctgga
     9181 ttattagttc tttttgcctt cttgtggggg gcaagaagaa agggttcctc ggtttcagta
     9241 tctgaattct ggcaagaaaa ggagaaagaa ttcggagaaa ataaaattct ttctggattt
     9301 tctcgctatt taggaggtca tcctcgtttt gaggcagtga gcgatggcct ccttttttta
     9361 atgtctaaga gtttgtggtt tgaaaacttt gaaaagggtc ctaacatttt tggcattact
     9421 cctccttttg aaaaagtaat ttttcgtatc cttctctcgc aaatagaaga agtggaatct
     9481 cttccagaaa aagagttgct aaaaaggggt tttgaaagcc aattgtggag atatcgtcgc
     9541 ctgtctcctc gcccttttta tcttgctgtt aagtataaag atgagtggga aagagagaga
     9601 actctctact ttgatagtat ggtcgatgcg gatacctgga aacaggagct ggaaagggct
     9661 aaaagcaatt atattccgga agaagaaata aaagaaagtg gagtttgccc ccagtgtggg
     9721 aaaaagatag atcctgattt taaactttgc ccttattgtg ggtgcaggct ttcttaagga
     9781 ggtaagatgt cttttcaagc aggacactat caggattttt ccaaggccac agggtgcacg
     9841 gtttttttat ttgaacagcc taaccgagca gtggctgcgg tgcgaggggg agctccggga
     9901 ggtcgagaaa ctgctactct ttttcccgga aacctggtag agggagtaga tgccatattc
     9961 tttagcggag gtagcgcttt aggtctttcc tgtggagaag gagtggtgga tttcttgcgg
    10021 gaaagaaaaa aaggatttaa gactccgggg ggaacagttc ccattgtagc tggagcggtg
    10081 atttatgatt tagaagtggg agaaccagct ttccccgata gagaatgggg atacaatgct
    10141 gcgttgtctt cagaaaaagt ggtaaaagag ggaactatcg gggcaggaac gggagctacc
    10201 gtgggtaaac ttatgggaat ggaaggagca gtaaagggag ggtttggaaa aggaagggca
    10261 aaagtagaag gggaggaaat atctgcattt gtggttacta attcctgggg aaatatctat
    10321 cacccccgaa ctggagagtt ggtggcaggg gttaaaatga aaggaagagg gacaacgttt
    10381 tctcctttca ataccacttt gactctggtg gtttttgatt ttttcctctc ccgagaagag
    10441 ctattctctc ttgtgggagt agttcactct gctctggcat cttgcattcg cccttttgcc
    10501 actctctatg atggcgatac catttttttg gtatctacgg ggaaaaagaa agtgagtaat
    10561 tacctatctt tgatttcggg gatttataaa agtgtcacag aaggagtttt gaattcggtt
    10621 accaaagcca cagggctggc tggaattctc gctaaaaatg atataatagt ttagttttac
    10681 cgctgaccac aggcagcgag ttggttgatt ggttagttag tgttttttct cgtctggtat
    10741 ctaaaaagaa ctggaacgga ggtgtttttt ttggagaaaa gaactaagaa gttcgtgttg
    10801 gctggtgttt tgggggcagt gtcagtgatt ttgagcctta ctcctttggg gcttattccc
    10861 gttcccaatc ttgcttctta cgctactacc atgcacatac ctgctataat agggggtatt
    10921 ataggtggcc cggaggtggg ggctttagtg ggattgattt tagcattttc cactctccac
    10981 ctgtttgccg gtaatgtttt ggcctgtgtc ttgccccgtc ttttgattgg agtggtagct
    11041 tattacatct ggaagtggtc ggggaaaaaa gactggggag tggttctggg aagcctgggg
    11101 ggaactttta tcaacaccat aggggtgctg ggcttaatgg tggtgacggg tttctttacc
    11161 tgggcgcagg ttttgcctgt ttttgtcctt aatgggagtt tggaattagt catttctggt
    11221 attatagtgc tacctttagt acgtatttta aggcgggtgg tgaagttttg agtagaattt
    11281 tagtaattga tgtgggaaat acccatacca cctggggagt gtatgaggat aaagcgcttc
    11341 tttctaactg gcgagtatcc acctccccct accgtacttc tgatgaatgg agctttttgc
    11401 ttcaaaacct cctggaaggg gaaggaatta aaagggctac tttagacagt gtggttgttt
    11461 cct
//
LOCUS       DAOPSS010000046        11435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4768_length_11435_cov_10.476977, whole genome shotgun
            sequence.
ACCESSION   DAOPSS010000046 DAOPSS010000000
VERSION     DAOPSS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4768_length_11435_cov_10.476977"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2716
                     /locus_tag="PKN31_04155"
     CDS             <1..2716
                     /locus_tag="PKN31_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99151.1"
                     /translation="VGAGDLQALLYETPLERLQWLETVSGAGEWKKKLYQISLRLEKI
                     ESRRRLLEERLKELGLQVEKWKLWAQEEEEYLSLEREWRETRALYIQEMIRLRQEKVS
                     LLKKEIEMEEEKIREGEEEKRKWEDIFSSLSIVWDKCQREKRNIENEIEEKKKEKKKE
                     EEKLYYLLTEMRENRKNSLRIKEKLSRWEVEINLWEERERKWREDFSFVENEIEEIDD
                     KKLMLQLEERENALRELETKKIHLRERERHWKEDREKLEARLRSEMRKEKRTEEEILE
                     LEDKIKRGEEDIRDIESKLERRKEEGKILEEKLEKGKVILRSVSQKINSRRKREQGEE
                     EWEKELNARGWSRRAVQALGWLWENQDIERESPDLELSSQEPGRWCVSSSAVPPVFWR
                     ECKKKEALHLFQSGELPDENLVTSDGSLIFLRSGFLLFPGQLISSAGEPRFYRSWKKR
                     EKKWQGKIASWENKVRDIAKEEKNEEEKLWKKKLETEKEKEKLAYREKEKEEREREIK
                     ELKKEIELITDKKSIEEKELVTIEEEIARVKENISQIRCCIKEREKEKIKKEERERER
                     EKILWEMQSLKKEAEEALEILKREEEEKRRYGERLSEEGNKIRVIEVFLEQKMKEQKQ
                     KEEEEKRLNEEIDLAKKKIQDIAKEREERERKLEKALILEEKLDSEIKEFSRELEKVG
                     SFCPALSSTRVDIKELEYSLQKKEELLRKKRFIPGAGEEFARVEERFSSLKEKNECCK
                     AGLHLVGEGVSLAQKEIRKSFQEFLYEVNERFSCFFKEIFPGGEAQLLFQGDKAEIEV
                     RLPGKRKQNLSLLSSGERSLVALCFLFAVFEVADLPFCFLDEVDANLDHANSLLLAQL
                     LKNISQDRQVVMVTHQEEVMEVADQIIGVTMNDPGVSQAVCLDGKSFQ"
     gene            2730..3647
                     /gene="ftsY"
                     /locus_tag="PKN31_04160"
     CDS             2730..3647
                     /gene="ftsY"
                     /locus_tag="PKN31_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOA99152.1"
                     /translation="MMEKETTWQRWVKGMGKMKEEVKEHFSQLWSKKETGEKDWEELE
                     ELLIQADMGPALSLEIVEEFREFKKSRGGDDWQEWLYQKLFSLLEGNDPSLFASAKEG
                     ELKAIILSGVNGVGKTTAAGKLAYLFRNKGEKVSLIAADTFRAAAMEQLEVWAQRAKC
                     RYFRGTEGADPGAVVFDGISSARHQGDTLVIIDTAGRSHVNKNLLAELEKVVRVTKKL
                     ILPSNLESILVIDALAGQNAFLQAQSLFPVADLSGIILTKWDSQAKGGIVFRIGKEFH
                     LPVKYLGVGEGIEDLVPFDAEEFVKTIVY"
     gene            3768..5105
                     /gene="ffh"
                     /locus_tag="PKN31_04165"
     CDS             3768..5105
                     /gene="ffh"
                     /locus_tag="PKN31_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOA99153.1"
                     /translation="MFEALTDKFNAIFKKLRSKGKLSEEDVDAILKELRMVLLESDVH
                     YRVAKDLTSRVRERAVGKEVMESVTPGEMVIKILWEEIRQILGGEARELNLSGSFPAS
                     VMMVGLQGSGKTTTCGKLGVYLKERGFFPLLVSTDTRRPAAKEQLRILAQTAKLEFFD
                     DPQASTPQEMIKKARDYAREKSLDVILVDTAGRLHVEEELLEELKELIEEEEFSERFL
                     VLDATTGQEAVKVAQSFESWVHPTGLILTKVDTDARGGAVLSIVAQTGWPVKLVGVGE
                     KLNQIEVFHPDRMASRIMGLGDTLTFIEKVDRAISEEEKKKLEKKIEKKELDFNDFLE
                     ELQRVKNVGSFQEILDMFPGQIRGMMSQVDGEKNLKRVEAIINSMTREERENPSLIGA
                     SRKRRIAQGSGTTVQEVNKLLKQFEDFRKLWKQMKKGRPLPSSKGRGFFGLGR"
     gene            5129..5386
                     /gene="rpsP"
                     /locus_tag="PKN31_04170"
     CDS             5129..5386
                     /gene="rpsP"
                     /locus_tag="PKN31_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOA99154.1"
                     /translation="MAVKLRLSRIGRKHLPYYRIVAIDSRKARDGRPIEILGNYSPLA
                     DPPLWSLRTERIEYWISQGAEVSDKVKALLKEAKKLEAQTK"
     gene            5383..5613
                     /locus_tag="PKN31_04175"
     CDS             5383..5613
                     /locus_tag="PKN31_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOA99155.1"
                     /translation="MKDLVEFIVKALVDHPEEVKVSEVEGEQSVIYEIRVAQEDMGKI
                     IGKQGRIARALRTIVKAAGVKRGKKTAVEILE"
     gene            5630..6133
                     /gene="rimM"
                     /locus_tag="PKN31_04180"
     CDS             5630..6133
                     /gene="rimM"
                     /locus_tag="PKN31_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOA99156.1"
                     /translation="MKKVLVGKVLKAQGLRGMVKVLPLTDFEERFSPGSSLWVGLPDA
                     GYRLMTVAEATDEGRTILVRFQEVSSRQSAEEIQGFFLAVEEKELKALPSNHFYYFEL
                     LGLKVVEKGELRGEVVAIVEGPSYDYLVVRKEEKEYDLPFIRAYVQEVNKEEGFIEVE
                     CPGGFWD"
     gene            6109..6834
                     /gene="trmD"
                     /locus_tag="PKN31_04185"
     CDS             6109..6834
                     /gene="trmD"
                     /locus_tag="PKN31_04185"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOA99157.1"
                     /translation="MPRRFLGLKLRIDVVTLFPQAIEPYLNASILGKAQEKGIVKIKV
                     HNLRSFTLDRYKTVDDYPFGGGPGMVLKPEPLWRAVKFIESYTVSTPRVILTSPQGSL
                     FSQEKARELAQEEHLLFLCGRYQGVDERVITLTGAEEISIGDYILSGGELAALVMIEA
                     TVRLIPGVLGDEESIKADSFYKEGLGPPQFTRPRVFQGLEVPEVLLSGNHALIEEWRK
                     KKAEEKTARIRPELLPVAKDKKE"
     gene            6871..7224
                     /gene="rplS"
                     /locus_tag="PKN31_04190"
     CDS             6871..7224
                     /gene="rplS"
                     /locus_tag="PKN31_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOA99158.1"
                     /translation="MDKARVLRAVESSYIKEVPDVRPGDTVRLTLKIVEGGKERFQKF
                     EGLVIGVRGSGINRSITVRKISFGVGVERIFPLHSPSIEKIEILRRGKVRRAKLYYLR
                     GRSRKESRIAEKREA"
     gene            7202..7765
                     /gene="lepB"
                     /locus_tag="PKN31_04195"
     CDS             7202..7765
                     /gene="lepB"
                     /locus_tag="PKN31_04195"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOA99159.1"
                     /translation="MQRSEKLKEENGEPKKKSVWRELIETILWALVIALLIRYFVIEG
                     YYIPSGSMEPTLVPGERVLVAKFIYHFTEPQRGDIIVFRYPLDSRKNLIKRVVGLPGE
                     TIKIENGLVYINGVPLEGELFARPYYDAGYYGQGEKVIPEDSYFVLGDNSSNSDDSRF
                     WGYVPRKNILGRAFLVYWPPQRIKILR"
     gene            7762..8370
                     /locus_tag="PKN31_04200"
     CDS             7762..8370
                     /locus_tag="PKN31_04200"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOA99160.1"
                     /translation="MIEEFLWERGYSSIGGVDEAGRGPLAGPLVAACVVLPRYCYLPG
                     VRDSKSLSPSQREKLYREICSSSLAIGIGMVGERFIDAAGIEKANVFVFQEAIYRASS
                     SRFPEFLILDWVKVPFLQTPSLSLPQAESKSLAVAAASVVAKVFRDEIMENFYQPLFP
                     HYSFSQHKGYGTSLHRREIEEWGLEDCHRLSFCRKYEKQRQG"
     gene            8348..8707
                     /locus_tag="PKN31_04205"
     CDS             8348..8707
                     /locus_tag="PKN31_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOA99161.1"
                     /translation="MKNRDKGKKGELIAQKWVKSKLRLEILETNFRSPWGEIDIIARD
                     RDTWVFIEVKTRESLKAGLPEEAVTREKQNKIRRSALFYLQKKGYNPEDVFFRFDVLA
                     IRKEKGKLQFFYYPGSF"
     gene            8722..10254
                     /locus_tag="PKN31_04210"
     CDS             8722..10254
                     /locus_tag="PKN31_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOA99162.1"
                     /translation="MLARVLSATVWGVDARLVEVEVDVASGLPTFQIVGLPDTAVQEA
                     RERVRSAIKNSGFFFPGGRVTVNLAPASLKKSGSEFDLPIALGVLAATGQMKFTSSSV
                     VAMGELSLSGEIKPINGALPVAHFLSLSGKNYRLFLARENTKEGALVENLEVYGFRHL
                     NEVCDFLQGKIDLLPVTLDRESLFSLEGVGEDLEEVKGQRGGKRALEIAAAGGHHLLL
                     IGPPGSGKTMLARRLPSLFPPLSLDEAIEVTKIHSVAGLLSEKRPLVSQRPFRAPHHT
                     ISDVALIGGGQWPRPGEVSLAHHGVLFLDEALEFRRNVLEALREPLEVGKITVARAST
                     TITYPASFTLALACNPCPCGYLGDTKKTCTCSYRQIARYRAKLSGPLLDRIDLQVEIP
                     RLDFSEIASEKKEESSQEVRERVKRAREIQAERFRGEKIRLNGQMSARYLRRFSSLGG
                     EAKKLLEASMESLALSARAYHRILKVARTIADLEQSETIRVEHVAEAIHYRSLDRKYE
                     EF"
     gene            10256..11338
                     /gene="dprA"
                     /locus_tag="PKN31_04215"
     CDS             10256..11338
                     /gene="dprA"
                     /locus_tag="PKN31_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOA99163.1"
                     /translation="MEDRNFWIAFNHVMGIGPIRFQKLLNVFGDAKTAWRAGVKELSE
                     IIGPGMAQKVEEERKKIEPEKLRERIESKNFKVLTLRDDFYPSLLRNISNPPFLLYMW
                     GDFDFNQLDLFISIVGTRRPTPYGRKITRILASELAKDKWVIVSGLALGIDGEAHSSV
                     VREGGITVGVLGSGLEVIYPSSHRRLAEEIVDGGGAIISEYPPLCPPRPENFPPRNRI
                     ISGLTQGVVVVEAPKRSGALITADFALEQGREVMAVPGSIFSPQSEGANNLIAQGAKL
                     IQNKEDILEALGFSSGKAKKSYGEDSPLSGEKGELFSFIDSSGVFKEELLQLTGWEEG
                     KFFRVLLSLELQGLVQESPGGKIFRL"
BASE COUNT         3487 a         1851 c         3065 g         3032 t
ORIGIN      
        1 tgtaggggca ggagatctac aagccctgct ttatgagaca cctctggagc ggttgcaatg
       61 gttagagacg gtaagcggtg ctggagagtg gaaaaaaaag ctgtatcaga tttctctccg
      121 tttggaaaaa atagagagta gaagaagact tctggaggag cgattaaaag agctgggcct
      181 tcaagtagaa aaatggaaat tgtgggccca ggaggaagaa gaatatctct ccctggagag
      241 ggaatggaga gaaaccagag cactctatat tcaggaaatg ataaggctcc gtcaggagaa
      301 agtttctctt cttaaaaaag aaatagaaat ggaagaagaa aaaataaggg agggagagga
      361 agagaaaaga aaatgggagg atattttttc ttctctttcc attgtttggg ataaatgcca
      421 gagagaaaag agaaatatag agaacgagat agaggaaaag aagaaagaga aaaagaaaga
      481 agaggaaaaa ctctattatc ttttaactga gatgagagaa aacaggaaaa actctctccg
      541 gattaaagaa aaactttccc ggtgggaagt ggagattaat ctctgggaag aaagagagag
      601 gaaatggaga gaagattttt cttttgtaga gaacgagata gaggaaatag acgacaaaaa
      661 gttaatgctt cagcttgaag aaagagaaaa tgctctccgg gagttagaaa caaagaaaat
      721 tcatctgcgg gaacgagagc gtcattggaa agaagacagg gaaaaactgg aagcccgtct
      781 ccggagtgag atgcgcaaag aaaaaagaac agaagaggaa attttggagc tcgaagataa
      841 gattaaaaga ggagaagaag atatcagaga tatagaaagt aaattagaga ggagaaaaga
      901 agagggaaag attttagaag aaaaactgga aaaagggaag gtaattctgc gaagtgtttc
      961 ccagaagata aatagccgaa ggaaaagaga gcaaggggag gaggaatggg aaaaagaatt
     1021 aaatgctcga ggctggtctc ggcgggcggt acaggctctt ggctggctct gggaaaacca
     1081 ggatattgaa agagaaagcc ctgatttgga actgtcttca caagagcccg ggcgatggtg
     1141 tgtttcttct tctgctgtgc ctcctgtttt ctggagagag tgcaagaaaa aagaagctct
     1201 tcatcttttt cagagtggcg agctgcctga tgagaatctg gtaacctccg atggaagtct
     1261 aatctttttg cgcagtggat ttcttttgtt tcctggacaa ttaatttctt cggcagggga
     1321 acctcgtttt tatcgctctt ggaagaaaag agaaaaaaag tggcagggta aaatagcttc
     1381 ctgggaaaat aaagtccggg atattgctaa agaagaaaag aacgaagaag agaagctgtg
     1441 gaagaaaaag ctggagacag agaaagaaaa agaaaaatta gcttaccggg aaaaagagaa
     1501 agaagaaagg gaaagagaga taaaagaact taaaaaagag atagagttaa taactgataa
     1561 aaaaagcatt gaagagaaag aactggttac aatagaagaa gagatagcga gggtaaaaga
     1621 aaatatctct caaatccgat gttgcataaa agaacgagaa aaagagaaaa taaaaaaaga
     1681 agaaagagaa agagaaagag aaaagatttt gtgggagatg caatccctga agaaggaagc
     1741 agaggaggcc cttgagattt taaagagaga agaagaggaa aaaagaagat atggagaaag
     1801 attgagcgaa gaaggaaaca aaatccgggt gatagaagtt ttcctggagc agaagatgaa
     1861 agaacaaaag caaaaagaag aagaggaaaa acgcttaaat gaagaaattg atttagctaa
     1921 gaaaaaaatc caagatatag caaaagagag ggaagagaga gaaaggaaat tagagaaagc
     1981 tcttatttta gaagaaaagt tagattcgga gataaaagag ttttctcgag aactggaaaa
     2041 agtgggcagt ttttgtcctg cactttcctc aacccgtgta gatataaaag agctggagta
     2101 ttctctccag aaaaaagagg aattgttgcg gaaaaagagg tttattcctg gagcaggtga
     2161 agaatttgct cgagtagaag aacgcttttc ttccttaaag gaaaaaaatg agtgctgtaa
     2221 agccggtctg cacctggtgg gagaaggagt ttctcttgct cagaaagaaa tcagaaaaag
     2281 ctttcaggaa tttctttacg aggtaaatga aagattttct tgctttttta aagaaatttt
     2341 ccccggagga gaggctcaac tcctttttca gggagataaa gcagaaatag aagtgcgtct
     2401 gccgggaaag agaaaacaga atttgtctct tttatccagt ggagaaaggt cattggtggc
     2461 cctgtgcttt ctgtttgcag tttttgaggt agctgattta cctttttgct ttctggatga
     2521 ggtagatgct aatttagacc atgccaacag tctgcttctg gctcaattgc tgaagaatat
     2581 ttctcaagac cgacaggtgg tgatggtaac tcaccaggaa gaagtgatgg aagtagcaga
     2641 tcaaataatt ggagtcacta tgaatgaccc gggggtttcc caggcagttt gtcttgacgg
     2701 gaaatctttt caatagaaaa aggagagata tgatggaaaa agaaactacc tggcagagat
     2761 gggtgaaagg tatgggaaaa atgaaggagg aggtaaaaga acatttttcc cagctatgga
     2821 gcaaaaaaga aacaggagaa aaagattggg aagagctgga agaattgctc attcaggccg
     2881 atatggggcc tgccttatcc ctggaaatag tggaagaatt tcgggagttt aaaaagtccc
     2941 ggggaggaga tgactggcag gaatggttat atcaaaaatt gttctctctt ctggagggaa
     3001 atgatccctc tctttttgct tctgctaaag aaggagaact aaaggctata attctttctg
     3061 gagttaatgg agtgggaaag accacagcag ctggcaaact ggcttatctc tttagaaata
     3121 aaggggagaa agttagcctc attgctgctg atacttttcg agctgcggct atggagcagc
     3181 tggaggtttg ggcacaaaga gcaaaatgtc gctattttcg ggggacagag ggcgcagatc
     3241 cgggagcagt ggtattcgat ggaattagct ctgctcgcca ccagggagac actttagtta
     3301 ttattgatac agcaggaagg tctcatgtca acaagaacct gcttgctgaa ttggaaaaag
     3361 tagtgcgagt tactaaaaag ttgattctcc cttctaacct ggagagtatt ttagttatcg
     3421 atgctttagc cggtcagaat gcttttttac aggctcaatc tctatttcca gtggctgacc
     3481 tcagtggtat cattctcact aaatgggata gccaggctaa agggggaatt gttttccgta
     3541 ttggtaaaga atttcatctt ccggtgaaat atttaggagt aggagaaggg atagaagatc
     3601 tggttccctt tgatgcggag gagtttgtga aaactatagt ttactgaaag aacaatgagg
     3661 ggtgcaccgt tttttggttg tttttaaaac aatttctcag ggtttcttga cagagaagag
     3721 cgagccgatt attatcttat atgtttcagt aaaaaggaga gttgttcgtg tttgaagctt
     3781 taactgacaa atttaatgct atttttaaaa agttgcggag taaaggaaaa ttatctgagg
     3841 aggatgtaga tgccattctt aaggaattga ggatggtact actggaatcc gatgtccact
     3901 atcgagtagc taaagacctt acctctcggg taagagaaag agcggtgggg aaagaggtga
     3961 tggagagtgt aactcctgga gaaatggtta ttaaaattct ctgggaggaa attcgccaga
     4021 tattgggagg ggaagcccgg gagctgaatc tttctggttc ttttcctgct tctgtgatga
     4081 tggtgggatt gcagggctca ggtaagacca ccacttgcgg gaaactggga gtatatttga
     4141 aagaaagagg tttctttcct cttctggtct ccactgatac ccgccgtcca gcagccaaag
     4201 aacagctaag gattcttgct caaacggcaa aattagagtt ttttgatgat cctcaggctt
     4261 ctactcctca agagatgata aagaaagctc gagattacgc tcgagaaaaa agtttagatg
     4321 taattttagt ggacactgca ggtagattac acgtagaaga agagcttctc gaggagttga
     4381 aagagctaat cgaagaggaa gagttttcgg aaagattttt agttttagat gctactaccg
     4441 gtcaggaagc agtgaaggta gcccagagtt ttgaaagctg ggtacatcct acaggactca
     4501 tcttaaccaa agtggacact gacgcccggg gtggagcggt tctatccatt gtggctcaaa
     4561 cagggtggcc ggtgaaactg gtgggagtgg gtgaaaagct caatcagata gaagtttttc
     4621 atcccgatag aatggccagc cggataatgg ggttgggaga cactttgacc ttcattgaaa
     4681 aagtggatcg agccataagt gaggaagaaa agaaaaaatt agaaaagaag atagagaaaa
     4741 aggagctaga ttttaacgat tttctggaag agttgcagcg ggtgaaaaat gtaggatctt
     4801 ttcaggaaat tctggatatg ttccccgggc aaatacgagg gatgatgtct caggtggatg
     4861 gagaaaaaaa tctgaaaaga gtggaagcta taatcaactc tatgactcgt gaagagcggg
     4921 agaacccttc tctcattggt gccagtcgca aaaggaggat tgctcaaggt agtggtacca
     4981 cagtacaaga ggtaaataaa ttactcaagc aatttgaaga ttttcgtaag ctatggaaac
     5041 aaatgaagaa aggccgccct cttccttctt ctaaggggag aggatttttt ggcttaggaa
     5101 gatagagacg aaaggagaag aatagaaaat ggcggtgaaa ttgagattgt ctcgaattgg
     5161 tcgtaagcat cttccctatt atcgaattgt ggccattgat tctcgaaaag cgagagatgg
     5221 tagaccgatc gaaattctgg gcaattattc tcccctggcc gacccacctc tgtggtctct
     5281 acggacagag cggatagaat actggatttc tcagggagcg gaagtgtccg acaaagttaa
     5341 agctcttctt aaagaagcga agaagctgga ggctcagaca aaatgaaaga cctggttgag
     5401 tttatagtta aagcactggt agatcatcct gaagaagtga aggtttctga agtagaagga
     5461 gagcagtcgg taatttatga aatcagggta gctcaggaag atatggggaa aatcatcggg
     5521 aagcaaggaa gaattgctcg agcccttcgt accattgtta aggcagcagg agtaaaaaga
     5581 gggaagaaaa cagccgtaga aattctggag taggtaataa taaaataagg tgaaaaaagt
     5641 tctggtggga aaggttctga aagctcaggg gttgcgaggg atggtgaaag tgcttcctct
     5701 cacggatttt gaagaacgct tttctcctgg aagttctctg tgggtagggc tacctgatgc
     5761 aggttacaga cttatgactg ttgcagaagc aactgatgaa gggagaacta ttttagttcg
     5821 ttttcaggaa gtttcttctc gccagtcagc agaggaaata cagggtttct tcttagctgt
     5881 ggaagaaaaa gaattgaaag ctcttccttc caaccatttt tattattttg aattgttagg
     5941 tcttaaggtg gtggaaaaag gagaattaag aggtgaggta gtggctattg tagaaggacc
     6001 ctcttatgat tatctggtgg tgagaaaaga ggaaaaagaa tatgaccttc cttttattcg
     6061 tgcctatgtt caggaggtga ataaagagga gggctttatt gaggtagaat gccccggagg
     6121 tttctgggat tgaaacttcg tatcgatgtg gttaccctgt ttcctcaagc tatagagcct
     6181 tacctgaatg cttctattct gggtaaggcg caggaaaagg gaatagtgaa aataaaagtt
     6241 cataatttac gcagcttcac tttggatcgc tataagacgg tagacgatta cccttttggt
     6301 ggtggaccgg gaatggtgct caaacctgaa cctctgtggc gggcagtgaa atttattgag
     6361 agttatactg tcagcactcc gcgagtgatt ttgaccagtc ctcaggggtc tttgttttct
     6421 caggaaaaag cccgggagct ggctcaagaa gaacaccttc ttttcctttg tggccgctac
     6481 cagggggtgg atgagcgagt gataacctta accggagcgg aggaaatctc cattggagat
     6541 tatatcctct ctggaggaga gctggccgct ctggtgatga ttgaggctac agttcgtctc
     6601 attcccggag ttctgggtga tgaagaatct ataaaggccg attcttttta taaggaagga
     6661 ctgggtcctc ctcaattcac tcgtccccga gtttttcaag gattagaagt accggaagtt
     6721 ttattgtccg ggaatcacgc tcttattgaa gagtggagaa agaaaaaagc agaagagaaa
     6781 actgcccgga tacgaccgga acttctgcca gttgctaagg ataaaaagga atgatatatt
     6841 atagaagatt tccctcagga ggaggttcaa atggataaag caagagtttt acgagcagta
     6901 gaaagtagct atattaaaga agttcctgat gtgcgcccag gagacacagt gcgattaacc
     6961 cttaagattg tagaaggtgg taaagaaagg tttcaaaaat ttgagggttt agtaattgga
     7021 gtgcgaggtt cgggaataaa ccgtagtatt acagtgagga agatttcctt tggagtggga
     7081 gtagagcgta tttttcctct ccattctcct tctatagaaa aaattgagat tctgcgtcga
     7141 ggcaaggtaa gaagggctaa actctattac ttaagaggca ggagtcgcaa ggagtccagg
     7201 attgcagaga agcgagaagc ttaaagagga aaacggagaa cccaaaaaga aaagtgtctg
     7261 gcgagaattg atagaaacta tcctctgggc ccttgttata gcgcttttaa tacgctattt
     7321 tgtgatagag ggatactata ttcccagtgg ctctatggag cctactttgg ttcctggaga
     7381 gcgggttctg gtggccaagt ttatttatca ttttaccgaa cctcaaaggg gagatattat
     7441 agtttttcgc tatcctctcg attctcgcaa aaatctcata aaaagagttg tgggtttgcc
     7501 cggagagacg attaaaattg aaaatggcct cgtgtatata aatggggttc ctctggaagg
     7561 agaacttttt gctcgtccct actatgacgc aggttactac ggtcaaggag agaaagtgat
     7621 tcctgaagat tcttactttg ttttaggtga taacagtagc aatagcgatg atagccggtt
     7681 ctggggatat gtccctcgta agaatatttt agggcgagct tttctggttt actggccgcc
     7741 tcagcgaatt aaaatattga gatgatagaa gagtttctct gggagagagg gtattcttct
     7801 attggaggag tggatgaagc aggaagaggc cctttggccg ggcctcttgt agctgcttgt
     7861 gtggttttgc ctcgctattg ttatctcccg ggggtgagag attccaagtc tctttctcct
     7921 tctcaaaggg aaaagctgta tcgagaaata tgctcttcct ccctggctat agggataggg
     7981 atggtggggg aaagatttat cgatgcagca ggcatagaaa aagccaacgt ttttgttttt
     8041 caagaagcca tctatcgggc ttcttcttct cgttttcctg aatttttgat tttagactgg
     8101 gttaaagtcc cctttcttca gactccttct ttgtctctac ctcaagcaga aagcaaaagt
     8161 ttggctgtgg cagcagcttc tgtggtggct aaagtgttca gggatgaaat tatggaaaac
     8221 ttttatcagc ctctttttcc ccattacagt ttttctcaac acaaaggata tggtacctct
     8281 ctccatcgaa gagaaattga ggaatggggt cttgaagatt gccatcgctt gagcttctgt
     8341 cggaaatatg aaaaacagag acaagggtaa aaagggagag ttgatagccc agaaatgggt
     8401 aaagagcaag ctgcgtctcg agattttgga gactaatttc cgttctccct ggggagaaat
     8461 agatataatt gctcgtgacc gtgatacctg ggtgtttata gaagtgaaaa cgagagagag
     8521 cctaaaggct ggacttccag aagaggcagt cacccgggaa aaacaaaata aaatccgtcg
     8581 ctctgctctt ttttatttgc aaaagaaagg ttataacccg gaggacgttt tttttcgttt
     8641 tgatgtttta gctatccgaa aagaaaaagg taagttgcaa tttttttatt atcctggatc
     8701 tttttagagg ggggtgtaat tttgttagcc agagtcttga gtgccacggt ctggggagtg
     8761 gatgctcgat tggtggaagt agaggtggat gttgcttcag gtcttcccac ttttcagata
     8821 gtaggtcttc ccgacactgc agtacaggaa gccagagaaa gagtgcgcag tgcgatcaaa
     8881 aatagcggtt ttttctttcc tggcggtaga gtaacggtta atttagcccc tgcttcttta
     8941 aaaaagagtg gaagtgaatt tgaccttccc attgctctgg gggtgctggc tgctactgga
     9001 cagatgaagt tcacctcttc ttcggtggtg gccatgggag aattatctct ttccggggag
     9061 ataaaaccga taaatggtgc tcttcccgta gctcattttc tttctttgag tggtaagaat
     9121 taccgattgt ttttagctcg agaaaatacc aaagaagggg cattggtgga aaacttagaa
     9181 gtttatggtt ttcgccacct taacgaagtt tgtgattttt tgcagggcaa aatagattta
     9241 ttgcctgtca ctttagaccg ggaaagtctt ttttctttgg agggagtggg agaagactta
     9301 gaagaagtaa aaggacagag gggagggaaa agagctttag agattgccgc tgctggagga
     9361 catcatcttt tattaatcgg tcctcctggt tcgggtaaaa ccatgttggc ccggagattg
     9421 ccttctttgt ttcctccttt atctttagat gaggcaattg aggtaaccaa aattcacagt
     9481 gtagctggtc ttctttctga gaagcggccc ttggtttctc agcgtccttt tcgtgctcct
     9541 catcatacca tctccgatgt ggctttgatt ggaggagggc aatggcctcg ccctggagag
     9601 gtgagtctgg ctcaccatgg ggttctgttt ttagatgagg ctctggaatt tcgacgcaac
     9661 gttttagaag ccttacgaga gcctttagaa gtaggaaaaa taacagtagc ccgtgcttct
     9721 accactatta cttatcctgc ttccttcact ctggcactgg cttgtaaccc ctgtccttgt
     9781 ggatacttag gagatactaa aaaaacctgt acctgttctt atcggcagat tgcgagatac
     9841 cgggctaaat tatcaggccc tctcctggac cgcattgatt tacaggtgga gatacctcgt
     9901 ttagattttt ctgaaattgc ttcagaaaaa aaagaagaaa gttctcaaga agtgagagaa
     9961 agagtaaaaa gagctcgaga aatccaggct gagcgtttcc ggggagagaa aatccgtctc
    10021 aatggccaga tgagtgctcg ctacctccgg cgtttctcat ctctgggggg tgaagccaaa
    10081 aaacttctcg aggcttctat ggaatcttta gctttgagtg cccgtgctta tcaccggatt
    10141 ctcaaggtag caagaaccat tgctgatctg gagcagtcgg agactattcg ggtagagcat
    10201 gtggcagaag ctattcatta tcgttcttta gatcggaagt atgaggagtt ttgaggtgga
    10261 ggacaggaat ttctggatag cttttaacca cgtgatggga attggtccta ttcgtttcca
    10321 gaaattatta aatgtttttg gcgatgctaa aactgcctgg agggcgggag tgaaagagtt
    10381 aagcgagata attggtcctg gtatggctca aaaagtggag gaagagagaa aaaaaatcga
    10441 gccggaaaag ttgagagaaa gaattgaaag taaaaatttt aaggttctca ccctgagaga
    10501 tgatttttat ccttctcttc ttcgtaacat ttctaatccc ccttttttgc tttatatgtg
    10561 gggtgacttc gattttaacc agcttgacct ttttatttct atagtaggca ctcgcaggcc
    10621 tactccttat ggaaggaaga taaccagaat tttagcttct gaattggcta aagataagtg
    10681 ggtgatagtg agcgggttag ctctgggtat tgatggggaa gctcacagta gcgtggtaag
    10741 agagggaggg attaccgtag gggttttagg aagtggtttg gaggttattt atccttcttc
    10801 tcaccggcga ttggctgagg aaatagtgga tggaggaggc gccataatta gcgagtatcc
    10861 tcctctgtgc cctccccgcc cggagaactt tcctcctcgt aatcgaatta tcagtggctt
    10921 aactcaagga gtggtggtgg tagaagctcc aaagcgcagc ggtgctctga ttacggcgga
    10981 ttttgctctg gaacagggga gagaggtgat ggcggttccc ggttctattt tttctccgca
    11041 aagtgaaggg gccaataatc tcattgctca gggagcaaag ctgatacaga ataaggagga
    11101 tattttagaa gctttaggtt tttcttccgg aaaggcaaaa aagtcttatg gagaggactc
    11161 gccgctttct ggagaaaagg gggagctgtt ttcttttatt gattcttccg gggtgtttaa
    11221 agaagagctg cttcagctta ccggctggga agagggtaaa tttttccgtg ttttactttc
    11281 tctggaatta caaggattgg tgcaggagtc accaggagga aaaattttcc ggttgtaagt
    11341 gagccaacta tataaataat cgggagagcc ataagttttt cgcttcgtaa ccatttttaa
    11401 ttctctttta cactttgccg tttcgggtgt caatt
//
LOCUS       DAOPSS010000047        11373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4797_length_11373_cov_9.029864, whole genome shotgun sequence.
ACCESSION   DAOPSS010000047 DAOPSS010000000
VERSION     DAOPSS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11373
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4797_length_11373_cov_9.029864"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..345
                     /gene="yajC"
                     /locus_tag="PKN31_04220"
     CDS             13..345
                     /gene="yajC"
                     /locus_tag="PKN31_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOA99164.1"
                     /translation="MMNWFISLAHAANEVPDAAAPAAPAAPSIIAQLFPLIFLILIFY
                     FLLIRPQQQRQKRQMELLNSIQKGDKVVTIGGIHGTVAEVDEDDILLEVDKNTKIRFS
                     KSAVATKE"
     gene            390..1577
                     /gene="secD"
                     /locus_tag="PKN31_04225"
     CDS             390..1577
                     /gene="secD"
                     /locus_tag="PKN31_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOA99165.1"
                     /translation="MRRGNLPWRLIVSVVLVVISLVVIIPVKQKIRLGLDLQGGSHIL
                     LECVDTPEAPVDSDAVKRVVEIIRNRIDQLGVAEPVIARQGERRVLVQLPGVTDPQRA
                     VEVIGRTALLEFKDENGNTLLTGAHLKNAQVQYDRFGRPVVSIAFDEEGAKLFAQATT
                     LNVGKPIGIYLDDNLISNPVVQEPIMGGEAQITGGFTLEEAQNLAILLRAGALPVKVE
                     VIENRSVEPSLGQDSITHGLRAAVVGALLVVVFMIIYYGWLGVLADLALASFAIFFLA
                     IFISLRATLTLPGIAGFIVTVGMAVDANVLIFERIKEEYRAGKTWRASIEAGFNKAFH
                     TILDSNITTLIAAVMMFYLGSGPVRGLGVTLTLGIICSMFTGIWVTRALVDFFAEWIK
                     FEA"
     gene            1600..2481
                     /gene="secF"
                     /locus_tag="PKN31_04230"
     CDS             1600..2481
                     /gene="secF"
                     /locus_tag="PKN31_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOA99166.1"
                     /translation="MELIKNPNLNFVGNRRLAYTISIIIIAVGLISLLVQGLNYGVDF
                     VGGTLLQFRFNREVEVEEVREALEGLDLAKSTVQRFSPQEVIIRSPKLSSEEQDEVEK
                     ILGEIFGEVELVRVEDVGPAVGADLRRMAVTAVLVALLGILVYVSIRFEFRPGVTSIV
                     ALAHDALVVLGMLSLFQREFSAPVLAAILTILGYSINDSIVVMDRVRENLALRKKGET
                     FGELVNRSINETLSRTVNTVLTTILPVLALLILGGEMLRDFSFTLLIGLIAGTYSSIF
                     VVSPLWVEWEERKPLRR"
     gene            2488..4185
                     /gene="recJ"
                     /locus_tag="PKN31_04235"
     CDS             2488..4185
                     /gene="recJ"
                     /locus_tag="PKN31_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOA99167.1"
                     /translation="MGKKWQIRKVNEGYLKRLSRSLSMPVSIARVLMNRGVFTLQEAR
                     DFLNPHLSFLQKLQELPNLKEAVLLLDKVIRERGKILIFGDYDADGLTAAAILARFLR
                     LFSSEVEIYIPSRFREGYGLNREAVESILKREPNLVVTVDCGIRDLPGIAALRNKGIL
                     VLITDHHLPDFDLWPQELCVVSTWDRDSREMISPLSGVGIALALAQAYGEYRGTSVIP
                     VEEYIDLACIGTVSDVVPLLEENRVIVRYGLEKLNRNPCCGLRALCEVSGLTGRTIGT
                     QEIGFILAPRINAAGRVEDPQPALALLLSDEYEEAIKLASYLDVLNSRRKKEEEKVLE
                     SVFQDEKNEAYLQDEVVVISGPEWNQGVLGIVASRLSEQLDRPVIALTEIDGVAMGSG
                     RSIAGFNLSRALECSSSLLIRYGGHEMAAGLRLPVDRVGELRDFLNDNFAEEVEKARS
                     RRGLVVDALVGLREVNEETLFWLEKLEPFGEKNPPPLFAVLDASLIRSWIWGRNDQHL
                     RLLVKQGRESQEMVVINGKKKAANLVNSSLVDLAFEVYRDNAGNYPYLKIQDWRIKK"
     gene            4203..4715
                     /locus_tag="PKN31_04240"
     CDS             4203..4715
                     /locus_tag="PKN31_04240"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOA99168.1"
                     /translation="MDLAQYIRNIPDFPQPGVQFKDITTLLKEGPAFRETIERLVGLF
                     EDKEIELVVGIEARGFIVGAPLAYSLGCGFVPVRKAGKLPAPTLSKTYDLEYGSATLE
                     IHRDAIEKGQKVIIVDDLLATGGTTRAVCEMVEELGGRIIGIGFVIELESLKGREKLS
                     SYPVHSLIRL"
     gene            4815..6956
                     /locus_tag="PKN31_04245"
     CDS             4815..6956
                     /locus_tag="PKN31_04245"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOA99169.1"
                     /translation="MDKVSLYHPDFNSDLVNSAFSFSLEAHRNQVRLSGEPFIIHPLA
                     VAHIVADLKMDENTIAASLLHDVLEDSEVTEEDLEKNFGEEITNLVKGVTKLNRGFSY
                     VSREEAEVENYRRLFIAMAQDVRVIIIKLADRLHNMRTLKYQYRDKKETIARETLEIF
                     APIANRLGINRIQTELEDLSLFFLDPGAYCNIQRGIERVKEAQEKQVEEAKQELEARL
                     KKAGIEAEIQSRFKHIYSIYNKLNRQKISLEDLPDLIALRVITREVEECYAVLGMVHT
                     LWKPVEGRFKDYIAVPKSNMYQSLHTTVIGPRGAPMEVQIRTWDMHQVAEYGVAAHWR
                     YKEGKTGESDDKFDERMSWLRQILEWQQDLRSTQEFMESLKISLFDDEVYVFTPKGDL
                     KKLPQGSTPIDFAYLIHTEVGNNCVGAKVNRQMVPLSYELKSGDIVEIITSRASSGPS
                     VDWLKIARTPGARNKIRAFLRKKNQDLNREKGREALEKELEKYSLTPEEKALVTKNLS
                     QFREENQLREEEELYIAIGEGRYSARQVANKVIPYTIRRRLQKKKKESSAVTREEERI
                     VVQGASGLAVKLARCCTPVPGDQIVAFVTKGKGITIHRVDCPNLTRLSQDGERFLEAE
                     WEEREGDKYNAGVVIEALDRPKLLADVSNAISSFQVGIKSVRASTVGNEARIYLILEV
                     KNRQELDDVFREVKKIANVRSVRRGGAFALR"
     gene            6953..7408
                     /gene="dtd"
                     /locus_tag="PKN31_04250"
     CDS             6953..7408
                     /gene="dtd"
                     /locus_tag="PKN31_04250"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOA99170.1"
                     /translation="MKAVIQRVKEARVRVEREVVGEIGRGLLVFLGVHREDGEKDAQF
                     LINKIPYLRIFEDEEGKMNRSVHEIGGEILLVSQFTLCGDCRRGLRPSFDQAASPPEA
                     QKWYDEIGKRWEEEGIKVERGIFGAFMEVEIHNEGPVTIILDSKERRNK"
     gene            7405..8055
                     /locus_tag="PKN31_04255"
     CDS             7405..8055
                     /locus_tag="PKN31_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA99171.1"
                     /translation="MTLQLEHFILGELSTNCYLIHDGAEAFLIDPATPSWEIKEEIET
                     RNLGLRYIINTHGHIDHIGGNAFFKEHFPQAQLVIGERDLIYLTNPDLNLSRDFSSPF
                     ISPLPDWVVRENNLFLNTPWGKLSFLLTPGHTPGSVCLFLPVENWLFSGDTLFAGSVG
                     RTDLPGGSFRDLISSLEEIFNSFPDTVSVFPGHGPFTNLAREKKENFYYLQYLARD"
     gene            complement(8052..9410)
                     /gene="glmM"
                     /locus_tag="PKN31_04260"
     CDS             complement(8052..9410)
                     /gene="glmM"
                     /locus_tag="PKN31_04260"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOA99172.1"
                     /translation="MRKLFGTFGIRRVANEVLTPEMATKLALSFGTFISGEEVVVGRD
                     SRKHSEMLYYAVISGLLSAGCSVTELGVTATPALQWACRRWNQWGAMITASHNPPQWN
                     GIKFMEKTGKGLDREKDEEVEKIYFEEQFTRVPGMKIQSETKKRDIRWDYIEAIIQKV
                     DAQAIREKKFRVALDCANGAPSLITPYLLSELGCQVVSLNAHPDGNFPGRNPEPVPEN
                     LQDLIALIKNSPFAVGFAQDGDGDRLIVIKENGEFVPGDLSVSLVARELAHRETPGPI
                     ATTVATTHILQEIARETNREVIITEVGDLVVAKALQEHGGIFGCEENGGMIFPDFVWG
                     RDGAMAAAQILHLMAREGKPLSELLKELPPYYQVKTRLEIDPHKREKILQEVERTIAE
                     EKNQKEIITIDGYKVIFEDGSWLLIRFSGTEPLVRVFAESKDKEKAENLVKKYLDLIK
                     GI"
     gene            9587..11215
                     /locus_tag="PKN31_04265"
     CDS             9587..11215
                     /locus_tag="PKN31_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99173.1"
                     /translation="MKRVLCSLILVLLLFFASGAGAYEILLYANLTQLPLEDGVVLRI
                     DLGGHELMEEEWGNILESSENIVLLPSLSDIEKRISFLEENKEKVLGIVVSDLFSEEQ
                     PVFSPFLSFTHNYHQVLIFNFLHDDPDVPWDEYLKETEEADSIIVVGTGGNLPLLREK
                     FSAEGEKVQFVERDSSPDFQEKLSVEEKPLLFFFYSSRCPSCRHLKDEVVPPVLDKYK
                     DKIKVIYLDYLFSENYKKLVEWEEHWQVENKTSVEAFSDAGFVAEEDEKEFASRLEEL
                     IQKTIESEKKEVSPPPPGEDLIFRRFEGFTPWVIIGAGFLDGLNPCAFATIVFMVNLL
                     LLLGHSRRRILEIGLTYSITVFVTYLLLGLGLFQFWHVLSAYQTASRILYLIMALLLL
                     VFAALSIKDAIQYKKKGKETDMTLGLPESWRRRINQYLKNSFTEKNLVVAAILSGFVI
                     SLLEAGCTGQVYLPTIMYIARETPYRWQALGYLLLYNAFFILPLLVVFFAIFWGSQSK
                     ALVDFARKNIVFSKIALAILFVCLSLLLLKGALF"
BASE COUNT         3064 a         2140 c         2768 g         3401 t
ORIGIN      
        1 aaggagtgat tgatgatgaa ctggtttatt tctctggctc acgctgctaa tgaagtacct
       61 gatgctgctg cacctgctgc gcccgctgcg cccagcatta ttgctcagct ttttcccctt
      121 atttttctga tccttatctt ctactttctt ctcatccgtc ctcaacagca gagacagaaa
      181 agacagatgg agctgttgaa tagcattcaa aagggagata aagtggtcac aataggagga
      241 attcatggaa cggtagctga agtggatgaa gatgatatat tattggaagt agacaaaaat
      301 accaagattc gcttttccaa gagtgctgtg gccactaagg aataggttat aattaataat
      361 atcagaagcc ggctggggag gaaaagaact tgagaagagg taatttaccc tggagactaa
      421 ttgtatctgt agttttagta gtgatatctc tggtggttat tataccagta aagcagaaaa
      481 ttcgcctggg tcttgatttg cagggaggtt ctcacattct gcttgagtgt gtggatactc
      541 ctgaggctcc tgtagacagc gatgcagtga aaagagtggt agaaatcatc cgtaaccgga
      601 tagaccagtt aggtgtagca gagccggtta ttgcccggca gggggagcgt cgagtgctcg
      661 ttcagttgcc gggggtcact gatcctcaaa gggcagtgga ggtgattgga cgtactgctc
      721 ttctggagtt taaagacgaa aatggtaaca cacttttaac cggagcacat cttaaaaacg
      781 ctcaagtgca atatgaccgc tttggaagac cggtagtttc catagctttt gatgaagagg
      841 gagccaagct ttttgctcag gctactactc ttaatgtagg aaaacccata gggatttatc
      901 tggatgacaa tcttatttct aatccagtgg tgcaagaacc tataatggga ggagaagcgc
      961 agattaccgg agggtttact ttagaagaag cgcaaaatct ggcaatatta ctccgggcgg
     1021 gagctctacc ggttaaggta gaggtgatag aaaaccgctc tgtggagcct tccctgggtc
     1081 aggattctat aactcatggg ctccgagcag cagtagttgg tgctctcctt gtcgtagtgt
     1141 ttatgataat ttattacggg tggttgggag tgctggccga tttagcttta gcttcttttg
     1201 ctatcttttt cctggctatt tttattagtt tgcgggctac tttgacttta ccagggattg
     1261 ccggtttcat agttactgtg ggtatggcgg tagatgctaa tgttctcatt tttgaacgga
     1321 ttaaagaaga ataccgggca gggaaaacct ggcgagcttc gatagaagcg ggattcaata
     1381 aagcttttca caccatttta gattctaaca ttaccacttt gattgctgca gttatgatgt
     1441 tctatctggg gtcaggtccg gtgcgaggtc tgggagtaac tctgacttta ggcatcatat
     1501 gcagcatgtt taccggtatc tgggttactc gagctttagt agatttcttt gctgaatgga
     1561 tcaagtttga agcttgaagg gccaggaggt agaaaataaa tggaattgat aaaaaatcct
     1621 aatctgaatt ttgtgggaaa ccgtcgccta gcttacacca tttctatcat tattatagca
     1681 gtagggttga ttagcttgtt ggtgcaaggg ttaaactacg gtgtagattt tgtcggagga
     1741 acacttctcc agtttcggtt taaccgggag gtggaagtgg aagaggtgcg ggaggcttta
     1801 gaagggttag atctcgcaaa aagcacagtg cagcgctttt ctcctcaaga agtaatcatt
     1861 cgttctccca agctcagctc tgaggagcaa gatgaggtag aaaaaatcct gggagaaata
     1921 tttggggaag tggagctggt aagagtagaa gatgtaggac cagcagtagg agcagatttg
     1981 cgtcggatgg cagtcactgc tgttttagtg gcacttctcg ggattctcgt ttacgtgtcc
     2041 atacgttttg aattccgtcc cggagttacc tctattgttg ctttagctca tgatgctttg
     2101 gtcgttttgg ggatgctttc tcttttccag cgcgaattca gtgctccggt tctggcagct
     2161 attttaacta tcctgggtta ttcaatcaat gattctattg tagttatgga tcgggtaagg
     2221 gaaaatttag ctttgcgcaa aaaaggagaa acttttggag aactggttaa ccggagcatc
     2281 aatgaaactc tttcccgcac cgttaacaca gttttaacca ctattttacc agtcctggct
     2341 cttttaattt tagggggaga gatgcttcgg gacttttcct ttactttatt aattggactt
     2401 atcgctggta cttattcttc catttttgtg gttagcccct tgtgggtaga atgggaagag
     2461 agaaagccat tgcggaggta gatatctttg ggcaaaaagt ggcagattag aaaggtgaac
     2521 gaaggttatt taaaacgctt gtctcgttcc ctttctatgc ctgtttccat tgcccgggtg
     2581 cttatgaacc ggggggtttt cactcttcaa gaagcacggg actttttaaa ccctcatctt
     2641 tcttttttgc aaaaactcca ggagttgcct aatttaaaag aggcggtttt acttttagat
     2701 aaggtgatta gagaaagagg aaaaattctt atttttggtg actatgatgc tgatggttta
     2761 actgctgcag ctattttagc ccgttttctg cgacttttct cttctgaggt agagatatat
     2821 atacccagtc gttttcgaga aggttacgga ctcaaccggg aggcagtaga aagtatttta
     2881 aagcgagaac ccaatcttgt cgtaacagtg gattgtggca tcagggactt acccgggatt
     2941 gcagcgctaa gaaacaaagg catcctggtc ttaattactg accaccatct ccctgatttt
     3001 gacctctggc cccaggagtt atgtgtagta agcacctggg atagagattc ccgggagatg
     3061 atttctcctc tctctggggt gggaatagct ttagctttgg ctcaagctta tggtgaatat
     3121 cgagggacca gtgtaattcc agtagaagaa tatatagatt tagcctgcat agggacggtg
     3181 agcgatgtgg ttcctcttct ggaagagaat cgagtcatcg ttcgttatgg cctcgaaaag
     3241 ttgaaccgga atccctgctg tggtctccgg gctctctgtg aagtttccgg cctcactgga
     3301 agaactatag ggacgcaaga gataggtttt attctggctc cccgtatcaa tgccgcaggt
     3361 agggtagaag accctcagcc ggctctggct ttactcttga gcgatgaata tgaagaggct
     3421 ataaaattgg cgagttatct ggatgtcttg aattctcgga ggaagaaaga agaagaaaag
     3481 gttttagaga gtgtttttca agatgagaaa aacgaagctt atcttcagga tgaggtggtg
     3541 gtaatctcag gaccagaatg gaaccagggg gttttaggta ttgtggcttc ccgtctcagt
     3601 gagcagctgg atcgaccggt gatagctttg actgaaatcg atggggtggc aatgggttcc
     3661 ggtcgcagca ttgcaggttt taatctctcg agggccttag agtgtagctc gtcacttctg
     3721 attcgttatg gtggtcatga gatggctgcc gggctccggt tacctgtcga tagggtaggg
     3781 gaattgcgag attttttaaa cgataatttt gccgaggaag tggaaaaagc tcgctccagg
     3841 cgggggttag tggtagatgc tctcgttggt ttgcgagaag ttaacgaaga gacccttttc
     3901 tggttagaaa agcttgaacc ttttggagag aagaatcccc ctcctctttt tgctgttctg
     3961 gatgcctctt tgattaggag ctggatatgg gggaggaacg atcaacacct ccgcttactg
     4021 gtaaaacagg gaagagaatc acaagaaatg gtggtgatta acggtaagaa aaaggcagcg
     4081 aatctcgtta attcttctct tgtagacctt gcttttgaag tatatcgcga taatgcagga
     4141 aattatcctt atttgaaaat tcaggattgg aggatcaaga agtgaaggag atagagttgg
     4201 atctggatct ggctcaatat ataagaaata tccccgactt tcctcagccg ggggtgcaat
     4261 ttaaagacat cactacctta ttgaaggaag gcccggcttt tcgggagact atcgagagat
     4321 tggtgggact ttttgaagat aaagagatag aattggtagt gggcatagaa gcccggggtt
     4381 ttattgtagg tgcgcctctg gcttattctc tgggttgtgg ttttgtgccg gtaagaaaag
     4441 cagggaagct ccctgccccc actctctcta aaacctatga tttggaatac gggtcggcca
     4501 ctctggaaat ccatcgtgat gccatagaga aggggcagaa agtgataatt gtggatgacc
     4561 ttctggctac gggagggact accagagcgg tttgtgaaat ggtagaagag ttggggggta
     4621 gaatcatagg cattggtttt gtcatcgaat tagaatctct gaaaggtaga gaaaaactat
     4681 cttcttaccc tgtccattct cttatccgcc tttagctctc cgggaatttt aagagatagc
     4741 ttttcgttat aaaatagaaa aattggggag gttagttctt tggctggagt ggggtagaga
     4801 ctatagaatc gctgctggat aaggttagcc tttaccatcc tgattttaat agtgatttag
     4861 taaattcagc tttttctttt tcccttgagg ctcaccggaa tcaggttcgc ttatccggtg
     4921 aaccatttat cattcaccct ttagcagtag ctcatatcgt tgctgatttg aaaatggatg
     4981 aaaacaccat cgctgcttct cttcttcatg atgttttaga agatagcgaa gtcactgaag
     5041 aagacctgga aaagaatttt ggggaagaaa tcactaacct ggtaaaggga gtaactaagc
     5101 taaaccgggg gtttagttac gttagccggg aagaggctga agtagagaac tatcgccgtc
     5161 tctttattgc tatggctcaa gatgtgcggg ttatcataat aaagttagct gaccgcctgc
     5221 acaatatgcg aacccttaaa tatcagtatc gggataaaaa agaaactata gcccgggaaa
     5281 cactggaaat ttttgctcct attgccaatc gcttgggaat caatagaatt caaactgagt
     5341 tagaagacct cagtcttttt ttcctggatc caggagctta ctgcaatatt cagcgaggga
     5401 tagaaagagt taaggaagca caggaaaaac aagtagaaga agcaaaacag gaattggaag
     5461 ctcgtttgaa aaaagcagga attgaagcag aaatacagag tagatttaaa catatttaca
     5521 gtatttataa taaattaaac cggcagaaaa tcagtttaga agacctgcct gacttaattg
     5581 cccttcgggt tatcacccgg gaagtggagg agtgttatgc cgttctggga atggtgcaca
     5641 ctttgtggaa accagtagag ggtagattta aagattatat tgctgttccc aagtctaata
     5701 tgtatcaatc tcttcacacc acagttatag gtcctcgggg tgcacctatg gaagtgcaaa
     5761 ttagaacctg ggatatgcat caggtggcag aatatggagt tgctgctcac tggcgttaca
     5821 aagaaggtaa aactggagaa agcgacgata agtttgatga gcgaatgagc tggctccgtc
     5881 aaatattaga gtggcaacaa gacctgagaa gcactcagga attcatggag agtctgaaaa
     5941 tcagcttgtt tgatgacgaa gtttatgttt ttacccctaa aggagatttg aaaaaacttc
     6001 ctcaaggctc tacccctatt gattttgcct atctcatcca cacggaagtg ggaaacaact
     6061 gtgtgggagc taaagttaac cggcaaatgg ttcctttaag ctatgagctc aaaagtggcg
     6121 atatagtaga aatcattacc tctcgagctt cttctggacc gagtgttgat tggttgaaaa
     6181 tcgccagaac tccgggggct cgtaacaaga ttcgagcttt cttacgtaag aaaaatcagg
     6241 acctcaaccg ggagaaagga agagaggctt tagaaaaaga gttagaaaaa tattctctca
     6301 ccccggaaga aaaagcgctg gtgacgaaaa atctttctca gtttagagaa gaaaatcaac
     6361 ttcgggaaga agaggaactt tatatcgcaa ttggtgaggg gagatacagt gctcgccagg
     6421 tggcgaacaa agtgattccc tatactattc gccgccgatt gcagaagaaa aagaaagagt
     6481 ccagtgctgt tacccgggaa gaagaaagaa ttgtggttca gggggctagt ggtctcgcgg
     6541 taaagttagc tcgctgttgt actcctgttc ctggagacca gattgtggct tttgttacca
     6601 agggaaaagg aattaccatt catcgggtag attgtcctaa tttaaccagg ctttctcagg
     6661 atggggagcg tttcttagaa gctgaatggg aagagagaga gggagataaa tataatgctg
     6721 gagtagtgat agaggctctg gaccgcccca aacttctggc tgatgtttcc aatgctatat
     6781 ccagctttca ggtgggaatt aaatcagtgc gagccagtac tgtgggaaat gaagctcgaa
     6841 tatatcttat tttggaagtt aagaaccggc aagagctgga tgacgttttc cgcgaagtga
     6901 aaaaaatagc caatgtgaga agtgttcggc gaggaggagc gtttgcctta agatgaaagc
     6961 agtaattcag agagtgaaag aagcccgggt cagagtagag agggaagtag tgggagaaat
     7021 tggaagaggt cttctggttt ttttaggagt acacagggaa gatggagaaa aagatgccca
     7081 attcttgata aataaaattc cttatctgcg catttttgaa gatgaggagg ggaaaatgaa
     7141 ccggagtgtg catgaaatag gaggagaaat ccttctcgtt tctcaattta ccctttgtgg
     7201 cgattgtcgg cgggggttac gtcccagctt tgaccaggct gcttctcctc ccgaagcaca
     7261 aaagtggtat gatgaaatag gaaaaagatg ggaagaagaa gggataaagg tagaacgagg
     7321 aatattcgga gcttttatgg aagtagaaat tcataacgaa gggccagtta ctataattct
     7381 ggatagcaaa gagaggagaa ataaatgact ttgcagcttg agcattttat tctgggagaa
     7441 ttgtctacca actgctatct gattcatgat ggtgcggagg cttttctcat cgatcccgct
     7501 actccttctt gggagataaa agaggaaata gagacacgga atctgggctt gcggtatata
     7561 atcaacactc acggtcatat cgaccatatc gggggcaacg ccttttttaa agaacatttt
     7621 ccccaggctc agctggtaat aggagaaaga gacctgattt atttgactaa cccggactta
     7681 aatttatccc gggatttttc ttctcctttt atttctcccc ttcctgactg ggtggtaagg
     7741 gaaaacaatt tatttctgaa cactccctgg gggaaacttt cctttcttct cactcctggc
     7801 cataccccgg gaagtgtttg tcttttttta cccgtggaaa actggctttt tagcggtgat
     7861 actctttttg ccgggtcagt gggaagaact gaccttcccg gggggtcgtt tcgcgatctc
     7921 atctcctcat tagaggagat ttttaatagc tttcctgaca ctgtttcagt gtttcccggc
     7981 catggccctt ttactaattt agcccgggag aaaaaagaga acttttatta tcttcaatat
     8041 ttagcccgag actaaattcc ttttatgaga tctaaatatt ttttgactaa gttttccgcc
     8101 ttttctttat ctttagattc ggcaaaaacc ctcaccaatg gttcagtgcc tgagaaacgg
     8161 attaataacc agctcccatc ttcaaaaatt actttgtaac catcgatggt gataatttct
     8221 ttttggtttt tttcctcagc tattgttctc tctacttctt gtagaatttt ctcccttttg
     8281 tgagggtcta tttccagtct ggttttcacc tggtagtaag gaggcaattc tttaaggagc
     8341 tcactcaacg gttttccttc tctggccatg aggtgaagaa tctgagctgc agccatggct
     8401 ccatctcttc cccacacaaa atcggggaaa atcattcctc cgttttcttc gcaaccaaat
     8461 attcctccat gttcttgcag ggctttagct accactaaat ctcccacttc agttatgatt
     8521 acttcccggt tagtttcccg ggcaatttct tgtaagatgt gagtggtggc tacagtggta
     8581 gcgatgggtc cgggggtttc tcgatgagct aattctcgag ctaccagact cacgctcagg
     8641 tcaccaggga cgaattctcc attttcttta atcactatta gccggtctcc gtctccgtct
     8701 tgagcaaatc ccacggcgaa aggagaattt ttgatgagag caattaagtc ttgtaagttt
     8761 tcaggtacag gttcgggatt tcttccggga aagttgccat cagggtgagc atttagactt
     8821 actacctggc atcccagttc gctcaaaaga taaggagtaa tgagagaagg agctccgtta
     8881 gcacaatcta acgccacccg gaactttttt tctcttatgg cttgtgcatc tactttttga
     8941 attatagctt cgatatagtc ccagcggatg tctctttttt tagtttctga ttgaattttc
     9001 atcccgggaa cgcgagtgaa ttgctcttca aagtatattt tttctacctc ttcatctttt
     9061 tctcgatcta aacctttccc ggttttttcc atgaatttta tgccgttcca ctgaggggga
     9121 ttgtggctgg cagtaatcat agctccccat tgattccatc gtcgacaagc ccactgcagg
     9181 gcaggagtgg cagtgactcc taattctgtt actgaacatc cggcagaaag tagcccggaa
     9241 atcacagcat aataaagcat ctcactatgt tttcgggaat cgcgacctac caccacttct
     9301 tctccggaga taaaagttcc aaaactgaga gctaatttcg tggccatctc gggagtgaga
     9361 acttcgttag ctactcttct gatgccaaaa gttccaaata atttcctcat cttcttcctc
     9421 ctctcgttaa aatagatatt aaattttctt tccctttagt tattttaact taaaagagaa
     9481 ataaatggta taaaacagtt tttaatcttc cagagctttt gattaaagat ataataatag
     9541 gagaaagttc attttaatta aaaaatatga tgaaaggatg gaagttatga aaagggtgct
     9601 ttgctctctt attctggttt tgcttttgtt ttttgcttca ggagcaggag cttatgagat
     9661 tcttctctac gctaacctaa cccagcttcc tcttgaggac ggagtagttt tacggattga
     9721 tttgggagga catgagctga tggaagagga gtggggaaat attttggaaa gctccgagaa
     9781 catagtgctt ttgccctctc tttcagatat agaaaagaga atctcatttc tggaagagaa
     9841 taaagaaaaa gtcttaggaa ttgtggtttc agacttgttt tctgaggaac aaccagtttt
     9901 ctcacctttt ctttctttta ctcacaacta tcatcaggtt ttgatcttta attttctcca
     9961 cgatgacccc gatgttccct gggatgaata tcttaaagaa acagaagagg cagattcaat
    10021 aatagtagtg gggacggggg gaaaccttcc tcttctccgg gagaagtttt ctgcagaagg
    10081 ggaaaaagta cagttcgtag aaagagattc ttctcctgat tttcaagaaa aactttcggt
    10141 ggaagagaag cctcttcttt tctttttcta ttcttctcgt tgtcccagtt gccgacactt
    10201 aaaagatgaa gtggttcctc cagtgttgga taaatacaaa gataaaataa aagtgattta
    10261 tctggattat cttttttccg agaattataa aaagctggtg gaatgggaag aacactggca
    10321 ggtagaaaat aaaacttcgg tagaagcatt ttctgatgct ggttttgtag ccgaagaaga
    10381 tgaaaaggaa tttgcttctc ggttagaaga gctaatccag aagactatag aatcggaaaa
    10441 aaaggaggta agtcctcccc ccccggggga ggacttaatt ttccgtcgat ttgaaggatt
    10501 cactccctgg gtaataatag gagcaggctt tttagacggg cttaatccct gtgcttttgc
    10561 cactatagta tttatggtga atttactttt gcttctggga cattctcggc gtcgtattct
    10621 ggaaattggt ttgacttaca gtataacggt ttttgttact tatctccttt taggtctggg
    10681 tctttttcaa ttctggcatg ttttatctgc ctatcagacc gcctctcgaa ttctttattt
    10741 aatcatggct ttattgcttt tagtttttgc tgctttgagt attaaagatg ctattcagta
    10801 caaaaaaaag ggaaaagaaa ccgatatgac actggggctc cccgagagct ggaggcgccg
    10861 catcaatcaa tatctaaaaa atagctttac tgaaaaaaac ttagtagtag ctgctatttt
    10921 aagtggcttt gttatttctc ttttggaggc agggtgtacc gggcaggtgt atcttcccac
    10981 cattatgtat attgctcgag agactcccta tcgctggcaa gcattaggat accttttact
    11041 ttacaacgct ttttttatcc ttcctcttct ggttgtgttt tttgctatat tctggggtag
    11101 tcaatctaaa gctttagtgg attttgcgag aaaaaacatc gtcttttcta aaattgcttt
    11161 agctatcctc tttgtctgtt tgagcttgct tcttctcaaa ggagctctgt tttagccata
    11221 ggtgcgcata aaagctaatg tcagaatagc cttggcagac tataggggga aaccccaccc
    11281 ctttatccgt catttctgaa tgcttctatc accttttgct cgtcaccccg gcaattttta
    11341 agccgttttt agcctgcccc tgaatgcttc tat
//
LOCUS       DAOPSS010000048        11060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4968_length_11060_cov_8.885597, whole genome shotgun sequence.
ACCESSION   DAOPSS010000048 DAOPSS010000000
VERSION     DAOPSS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11060
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4968_length_11060_cov_8.885597"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..637
                     /locus_tag="PKN31_04270"
     CDS             452..637
                     /locus_tag="PKN31_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99174.1"
                     /translation="MGIQMFFNTVDPSQNHSGVTNKGVIKAFRNRDKGMIEPFRGDDK
                     EGVIGSFLLIVWIIKEF"
     gene            814..1686
                     /locus_tag="PKN31_04275"
     CDS             814..1686
                     /locus_tag="PKN31_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOA99175.1"
                     /translation="MRSMTGFGYVEGAGELGFYKIRLKTLNHRFLELSCRIPREMGMW
                     EDKITSLVKERISRGKVEVRVEFEPREGAFSVEANVVLARSYWKALQKIVQELSLLQQ
                     PDLSMLLQVGEIVQIKEESSLWEEEWENFYPLLDQAIQSLVAYREAEGKRLREDLLFW
                     LKELERLTEEVEKRAPLVQTYYRNKLLQRIEEFLPQVKVDEMLLAQEVIFYVEKSDIN
                     EEIVRLKAHINRMNKLLSQNKSIGRELEFILQEMNREVNTMGSKSCQVEVSSLVVEMK
                     TTIEKMWEQVRNVE"
     gene            1690..2256
                     /gene="gmk"
                     /locus_tag="PKN31_04280"
     CDS             1690..2256
                     /gene="gmk"
                     /locus_tag="PKN31_04280"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOA99176.1"
                     /translation="MSGLLFVLSGPSGVGKSTLRKEVMKRCPALKYSISYTSRAPRRD
                     EKEGVDYYFVDMETFEEMKEKGEFIEWAKVHGNYYGTPRRQVEEWLKEGFDVILEIDV
                     QGAKQVKKNCPDGIFIFIAPPSLKTLEQRLRERNSDPEDEILLRMTNAHLEMQSIRDY
                     NYLVVNDVLEEAVDKLRSIIIAERCRIR"
     gene            2266..2460
                     /gene="rpoZ"
                     /locus_tag="PKN31_04285"
     CDS             2266..2460
                     /gene="rpoZ"
                     /locus_tag="PKN31_04285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOA99177.1"
                     /translation="MLSIDELVQKVGNIYILAIVIAKRAKQLNEGAVPLVELKEAHSP
                     LFVALEELSAGKISFEFVEE"
     gene            2457..3674
                     /gene="coaBC"
                     /locus_tag="PKN31_04290"
     CDS             2457..3674
                     /gene="coaBC"
                     /locus_tag="PKN31_04290"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOA99178.1"
                     /translation="MIVYHPTQFWKGKKIVLGVTGGIAAFKAISLASYFTKAGARVKT
                     CLTENALSLVGKSSFEAVTRERAYVGLFEGGGEILHIDLVRESDAVIVVPATANILGK
                     AASGIGDDLLSTILLVDPSKVILAPAMNVTMWKNPVVQDNLEKVVSRGMKVIYPASGS
                     LACGEEGEGRLPELEELVEGIFYHLYPRTEFQGKKVLISAGATREWLDPARFISNPAS
                     GFMGGALASLARACGGEVKMVVGNVSSRLPSGVEIERVETAQEMEIALKREWGNSDIL
                     FMNAAVSDFRPLSFSPSKIKKEGKEELTLTLVKNPDILSELAKNKGNKIVVGFCAETD
                     NPQEEARKKLQKKNADIIVANLIACGESGFETSTNKAWIVDCRGGEKELPLLDKRDLA
                     EEIVSYLFDHFRG"
     gene            3671..5689
                     /locus_tag="PKN31_04295"
     CDS             3671..5689
                     /locus_tag="PKN31_04295"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99179.1"
                     /translation="MNAHFALLALPLPLFSEFSYLIPPSLAGKIQVGSLVEVEFKNRR
                     LLGWVVALQEESPPGLEIKEILRLAPVAPLSAGGLLWTKILSQLYLTPWGMMLSFFTP
                     ALLPSFWDSAGKRVFPGSHTEASEESFSIPPQGMNFFVFQEKFGRKKKKWEKLIKNGV
                     LRREEETLEVAETTYPRKDSGWWSIATRKERESFLLSFVEKMQEENRKALLVFPDFRA
                     LDYFTSFVENQFPQLRVIKYDSRLASRRRFLAYKLAEEGDFDLILGTRGSIFLPARDL
                     YYHVVFDPEDKGHYSDRPPFHDTFRLLHEKVKIKGGSLEVVGITPSLSMYHFLSTQEF
                     RKRDFPVLSRASAPPVNLISYEKKWGSSLTFSVRKAILQVLNEKGRVLIWVQKRGYTP
                     ALGCRDCGFYYVCPHCGVAFRYYKETRSLICPLCGKKEEIDLFCPRCGGVWLEGWGEG
                     VEKVGEEMKKAFSHQQVVVIDSEKEGEVEEPLSSSILVGTSLLLREEILQGASLLVIW
                     SLEDWLYLPDINAKEEFYLSLQRIQALLGLASPVPPRIFIQGRKGMEKEMSQFLQPPR
                     DFYPPLLEKRKILGYPPYGVLARVVKRYRNRGKDELVLRKLKSSLEEKGIEVGGPFPA
                     QGRKWGEESEEITIHYTRERAEEVFNVVSQFFSREGKRKGEWRIEVEF"
     gene            5707..6210
                     /gene="def"
                     /locus_tag="PKN31_04300"
     CDS             5707..6210
                     /gene="def"
                     /locus_tag="PKN31_04300"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOA99180.1"
                     /translation="MAIREIKTYGSPVLRAKAQPIENVNQEVWNLAQDMKETMRLAKG
                     VGLAANQVGVLWRIITFTNPEEQKDQVLINPQIVHLSEEREKDEEGCLSFPDIYDQVE
                     RAKKVVVKGWDLEGKEITIEGEGLLARILQHEIDHLDGILFVDRLSPARRLLIRNKLK
                     RISVAEE"
     gene            6214..7140
                     /gene="fmt"
                     /locus_tag="PKN31_04305"
     CDS             6214..7140
                     /gene="fmt"
                     /locus_tag="PKN31_04305"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOA99181.1"
                     /translation="MKIVFMGTSSFAVLVLEELLKKEENNILGVVTKVDRPAGRGRKL
                     RPSPVKKKAEEEGLTIWQPERVNAPEFLDILRCVSPEVIIVAAYGQILRRDLLSIPPR
                     GAINVHASLLPSFRGPDPIRSALLQGEKETGVTIMLMDEGVDTGPILAQKKVEIERED
                     NYQSLTEKLGKEGGKLLVETLPRWARGEIAPRPQEGEVSYAPILKKEKARINWEDSAV
                     NIVNQVRAFSPYPGAYTYFQDRRIKILKANSKEGVSAPPGTVVEINPQGFVVAAGEGG
                     VLVEEVQPESRQVMTSREFCCGYHLREGEQLL"
     gene            7153..7827
                     /locus_tag="PKN31_04310"
     CDS             7153..7827
                     /locus_tag="PKN31_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOA99182.1"
                     /translation="MFFDYTFILLIPALILAFYAQAKVSSAYSKLSKKRASAGLTGRE
                     VAEMLVESLGLRVKVEEIPGALTDHYDPTREVLRLSQAVANGSSIADYSIVAHEVGHV
                     LQKREQYPAFAFRSALVPVASFGSQLALPLFLIGLIFYLPALMNIGIIFFSLAVLFQL
                     VTLPVEYNASSRAYLVLDKAGLVTAEEKPYIKQMLNAAALTYVAATAMAALQLLRLVL
                     LRGSRD"
     gene            7828..9105
                     /locus_tag="PKN31_04315"
     CDS             7828..9105
                     /locus_tag="PKN31_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOA99183.1"
                     /translation="MIDYRRRDALQILNRLYTGRVSFLDAEYSRWIKKRNYTEKDRGW
                     LVSLLRNIVKHQSFIDGVLKEYLPRWGKLPMAIQNLLRIGVAELLFHSSPSFAVVNEV
                     VEITKDTGAEFTALVNAVLRKIARKRDYYSAILEEEGMELPPFLEEEWKSWIGEEDLK
                     LLKKSLSLPPPLYVRVNTLKIGEDELRERWREKGIESSKTVLPGALEVDADYSTLLAL
                     PEYQEGLFYPQDLGSQLVVKTISPFIRGRIIDVCCGTGGKALTMAQYLSGGEVFAWDK
                     KEGKVRTLSKFISLFNLGKIKPMVVDTLRPPREFFSTADLVLVDAPCSGWGTIRRNPE
                     ILLKNPDLEDLSRQQFSLLLSASSLVKKGGVMVYCVCSITKKETQKVVKEWELNAGKE
                     WEKVGLGKGEEEMIIWPHQFNSDGFFVAAWRKK"
     gene            9102..10163
                     /locus_tag="PKN31_04320"
     CDS             9102..10163
                     /locus_tag="PKN31_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOA99184.1"
                     /translation="MKETKRENWSSFILRVFAYALIFGGGLALSVYLGLIFFQYYLTS
                     GTISLPDFRNQSLVEAINQISQLGLQAEIKRIENNPEFPSSWVIQQDPPPGVEVKKNH
                     RVKLVINGGDIIAEGGSVATGGSSSPSTSTSVSASGSVSVPDVRQMGLEEAQSLIESS
                     GLKLERVAEITHDSIPAGYVVSQNPPPESEVAKGSAVSLLVSKGKGEAEEFNLVVVPD
                     LVGLRLEEAKRLLAQSNLTVGNVEEIAVKGKDDGVVMRQSPAPGEEVEPGEKIHLQVT
                     KAEEEEAGLKELSLRFVLPSSRSPIKVEVVVTDELGERVVYEREHQGEEMVELTTTTK
                     GEGKVVIYLNGYYYWDKTL"
     gene            10185..10850
                     /gene="rpe"
                     /locus_tag="PKN31_04325"
     CDS             10185..10850
                     /gene="rpe"
                     /locus_tag="PKN31_04325"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOA99185.1"
                     /translation="MAKLAPSILSCDFSQLREELLAAEEGGAEIIHVDVMDGHFVPNF
                     TFGLPILKAVRKILPRVTIDVHLMTDNPRLYIADFVREGADMISFHLEASADYSLIVD
                     EIVEYGGKPGVALAPGTPWQCLSPILDKLYYVHLMTVHPGFGGQKFIPGMEDKIASLR
                     QEIRRRGLSTEIEIDGGVNDQNIEYLASCGASIIAAGSLVFAHQENIAARVRMLSEKI
                     RPY"
BASE COUNT         3136 a         1968 c         2848 g         3108 t
ORIGIN      
        1 ttctttatta tatcacctcg cctctcttcc gtcatttctc aggggtttcc tcatctctca
       61 tgcctgaaat ttagcgaggc atccacctcc cttccgtcat tcccgaaatt ttttatcggg
      121 aatctccccc ttccgtcctc ccattgggaa tcataagggt gtctcacgcc attgtttaga
      181 ttcctgatga atacgttcag gaatgacact ccttcccgcc gtccttcccg aaatccttaa
      241 tcgggaatct cccccttccg tcattcccga aattttttat cgggaatcca cgtttttgct
      301 ttttcctttg atttttaata ctgtggaccc ctgataaaac cattcagggt gacgacctcc
      361 ccctccgtca ttcccgaaat tttttatcgg gaatccagat gctttcaaat actgtggacc
      421 ccggcttaaa gattgccggg gtgacgagga agtgggaatc cagatgtttt ttaataccgt
      481 ggacccctcg cagaaccatt caggggtgac gaacaagggg gtgataaagg cattcaggaa
      541 tagagacaag gggatgatag aaccattcag gggtgacgac aaagaggggg taataggaag
      601 ttttctcctt atagtttgga tcattaagga gttttaagga atggttcaag atctgtaaag
      661 gtagatatac atcttgagta tgaggaatta tactttgtaa aaggtggttg ttttaaacaa
      721 cctcggtaac gattgacagg gttaaactta ttaaataata tggtatcata gttcagactc
      781 tacctacctg attttagaag gagcgtaaat tcgatgagaa gtatgaccgg ttttggatac
      841 gtagaaggag caggagaatt aggtttttat aaaattcgtc tgaaaacttt aaaccaccgt
      901 tttctggaac ttagttgccg tattccccgg gagatgggca tgtgggaaga taaaataaca
      961 agtctggtaa aggagcgtat ttcccgggga aaagtggaag taagagtgga gtttgagccc
     1021 cgggaaggag ctttttcggt agaggctaat gtggttttag ctcgttccta ctggaaagct
     1081 ctccagaaga ttgtgcaaga gttaagtttg cttcagcaac cagacctttc tatgcttttg
     1141 caggtagggg aaattgtgca gatcaaagaa gagagctctc tgtgggaaga agaatgggaa
     1201 aatttttacc cccttcttga ccaggctatc caatctcttg ttgcttatcg agaagcggaa
     1261 ggaaagcgtc ttcgggaaga ccttttattc tggttaaagg agctggaaag attgacggag
     1321 gaagtggaaa agagggctcc tctggttcaa acttactatc gcaacaaatt acttcagcgg
     1381 atagaagagt ttttacctca agtaaaagtt gatgaaatgc ttctggctca agaggtaatt
     1441 ttttatgtgg aaaaaagcga cattaacgaa gaaatagtga gattgaaagc tcatatcaat
     1501 cgcatgaata agctcctttc tcaaaacaaa tcaataggta gagagttgga atttattctt
     1561 caggaaatga atcgagaggt taacactatg ggctcgaaat cctgtcaggt agaggtttcg
     1621 tctttagtgg tggaaatgaa gacgacaata gagaaaatgt gggaacaggt tcgcaatgtg
     1681 gagtgaatga tgagcggatt actctttgtc ctctctggac catcgggggt gggtaaaagc
     1741 actcttcgaa aagaagtgat gaagaggtgt ccggctctga agtattctat ttcctacact
     1801 tcccgtgccc ctcgccggga tgaaaaagaa ggagtggatt actattttgt agatatggaa
     1861 acttttgagg aaatgaagga aaaaggggag tttattgagt gggcaaaagt tcacggtaac
     1921 tactatggta ctcctcgccg tcaggtggaa gaatggctaa aggaaggatt tgatgtgatt
     1981 ttagaaattg atgtccaggg agcaaaacag gtaaagaaga attgtccgga cgggattttc
     2041 atttttattg ctcctccttc tcttaaaact ttagaacaga ggctcaggga gaggaattct
     2101 gacccggaag atgagattct attgcgtatg actaatgccc atcttgagat gcaatccatc
     2161 agggattaca attatttagt ggttaacgat gttctggaag aagcagtgga taagcttcgt
     2221 tccataatta ttgctgagag atgtaggata aggtgaggtg agaaaatgct ttctattgat
     2281 gaactggttc aaaaagtagg taatatatat attcttgcta tagtgatagc caaaagagcc
     2341 aagcaattaa acgaaggtgc tgtacctctt gtagaactaa aagaggctca tagtcctcta
     2401 tttgttgctc tcgaggaatt aagcgccggg aaaatcagct ttgaatttgt agaagaatga
     2461 ttgtttatca tcccacccaa ttctggaaag ggaaaaagat tgtcctggga gtaacgggag
     2521 ggatagcggc ttttaaggct ataagcctgg ccagctattt caccaaagct ggtgcccggg
     2581 taaaaacctg tttgactgaa aacgctctat ctctggtggg taagagctct tttgaggcag
     2641 tgacgagaga gagagcttat gttggtctct ttgaaggggg aggggaaatc ctccatattg
     2701 acctggtgag ggaaagcgat gctgtaatcg ttgttccagc cactgccaat attctgggaa
     2761 aggcggcttc tggtattggc gacgaccttt tgagcaccat tcttttagta gacccgagca
     2821 aagttatttt agctcccgct atgaatgtta ccatgtggaa aaacccggtg gtgcaggata
     2881 acctggaaaa agttgtgagc cgggggatga aagtgattta tcctgcttca ggaagtctgg
     2941 cctgtggaga agaaggagaa ggaaggttgc cggagctcga ggaattagtg gagggaattt
     3001 tttatcatct ttatccccgc acggaattcc aggggaagaa agttttaatc agtgctggag
     3061 ccacccgaga gtggttagac ccggcccggt ttatttctaa cccggccagt gggtttatgg
     3121 gaggagctct ggcgtcactc gcgcgagcct gtgggggaga ggtaaaaatg gtggtgggca
     3181 atgtctcttc tcgtcttccc tccggggtgg agatagagag agtagaaacc gcgcaggaga
     3241 tggaaattgc actaaaaaga gagtggggga atagtgatat tttatttatg aacgctgcag
     3301 tttctgactt tcgccctctt tccttttctc cttctaaaat taagaaagag gggaaagaag
     3361 agttaactct taccttagtt aaaaaccctg atattctatc tgagttagct aaaaacaaag
     3421 ggaataaaat agtggtaggt ttctgtgctg aaacagataa tcctcaagaa gaggcccgga
     3481 aaaaattgca aaagaagaac gcagatataa tagtggctaa cctcatcgct tgcggagaga
     3541 gtggatttga gacctctact aataaagcct ggatagtaga ctgccgggga ggagaaaaag
     3601 aattacctct tttagataaa agagatttag cagaggaaat agtttcctac ctttttgacc
     3661 acttccgggg atgaatgcac attttgcctt gcttgctttg cctcttcctc ttttttctga
     3721 gttttcttat ctaattcctc cttctctcgc agggaaaatt caagtgggga gcctggtaga
     3781 ggtggagttt aaaaatcgcc ggcttctggg atgggtagta gctttacagg aagaatctcc
     3841 tccggggtta gagataaaag aaatattgcg attggctcct gttgctcctt taagtgcagg
     3901 agggttgctg tggaccaaaa ttctttctca gctttattta actccctggg gaatgatgct
     3961 ttcttttttt actccggcac ttttgccttc tttttgggat tcggctggta agagagtttt
     4021 tcctggctct cacactgagg cgtctgaaga gagctttagt attcctcctc agggaatgaa
     4081 tttttttgtt tttcaggaga aatttggcag aaaaaagaaa aaatgggaga agttgataaa
     4141 aaatggggtt ttgcgccggg aggaagaaac tcttgaggta gcagaaacca cttatcctcg
     4201 aaaagattca ggttggtggt ctatcgctac ccgtaaggag cgggagagct ttcttctttc
     4261 ctttgtggaa aaaatgcaag aagagaatcg gaaagccctt ctcgtgtttc ccgactttag
     4321 agcgcttgat tatttcacct cttttgttga aaaccagttt cctcaattga gagtgataaa
     4381 atacgatagt cggctcgcct ctcgccggag gtttctggct tataaactgg cggaagaggg
     4441 agatttcgat ttaattctgg gcactcgagg aagcatattt ttgcccgccc gagaccttta
     4501 ttaccatgtg gtttttgatc cagaagataa agggcattat tccgaccgtc ctcctttcca
     4561 tgacactttt cgactccttc atgaaaaagt taagattaaa ggaggaagtt tagaagtagt
     4621 gggcatcacg ccttctcttt ctatgtatca ttttctgagc actcaagagt tccgaaagag
     4681 agattttcct gttctctccc gggcttctgc tcctcccgtt aatttaattt cttatgaaaa
     4741 aaagtgggga tcttctttaa ctttttcggt gcgaaaagct attctccagg tgttaaatga
     4801 aaaaggcaga gttttaatct gggtacagaa gagaggctat accccggctc tgggctgtcg
     4861 agattgcggt ttttattatg tttgccctca ctgcggagtg gcattccgct attataaaga
     4921 aaccagatct ttaatatgtc ctctgtgtgg taaaaaagaa gaaatagatt tattctgccc
     4981 tcgatgtgga ggagtatggc tggagggatg gggagaggga gtggaaaaag taggagaaga
     5041 gatgaagaaa gccttttctc atcagcaagt agtggttatt gattcagaaa aagaaggaga
     5101 agtggaagaa cctctttctt cttccatcct ggtgggcact tctttacttc tacgggaaga
     5161 aatactgcag ggggcatctc ttttggttat ctggtcgctg gaggactggc tgtatcttcc
     5221 tgatataaat gctaaagaag aattttattt gagtctgcaa cggatacagg ctctcttagg
     5281 tcttgcttct cctgttccac cccggatttt tattcaaggg agaaaaggga tggaaaaaga
     5341 aatgagtcag tttttgcagc cacctcgtga tttttacccc cctcttttag aaaaaagaaa
     5401 gattttaggc tatccccctt atggggtact ggctcgcgtg gtaaaaagat ataggaatag
     5461 agggaaagat gaattggttt taagaaagtt aaagagctct ttagaagaga agggtataga
     5521 agtaggtggt ccttttccgg ctcaggggag aaagtgggga gaagaaagcg aggaaataac
     5581 tatacattat acccgcgaga gagcggagga agtatttaat gtggtttctc agtttttttc
     5641 tcgagaaggg aaaagaaaag gagaatggcg gatagaagta gaattttgaa aaaggggtga
     5701 agttagttgg caatcaggga aataaaaact tatggcagtc ctgtattaag agcgaaagca
     5761 caacccatcg aaaatgtaaa tcaagaagtg tggaatctgg ctcaggatat gaaagagacc
     5821 atgcgtcttg caaaaggagt ggggttggcg gcaaaccagg tgggagtttt gtggcgcatt
     5881 ataactttta ccaatcccga agagcaaaaa gaccaggttc tcattaaccc tcagatagta
     5941 catctgagcg aggaaagaga aaaggatgag gaagggtgtt tgagctttcc cgatatctat
     6001 gaccaggtgg aaagagcgaa aaaagtagtg gtaaaaggct gggatttaga aggaaaggaa
     6061 attaccatag aaggggaagg gctcttagct cgtattttac agcacgaaat tgaccatctg
     6121 gatggaatct tgtttgtaga ccgattgagc cctgctcggc gtttgctcat ccggaataag
     6181 ttgaaaagaa tttctgtagc ggaggaataa aacttgaaga tagtttttat ggggacttcc
     6241 tcttttgccg tcctggtgct ggaggaactt ctaaagaaag aagagaataa tatactggga
     6301 gtggtgacta aagtggatcg cccggcggga agggggagaa aattacgtcc ctctccagtt
     6361 aagaaaaagg cagaagagga agggctaacc atctggcaac cagagcgagt gaacgctccg
     6421 gagtttttag acattttgcg ctgtgtttct ccagaagtta taatagtagc tgcctatggg
     6481 cagattttgc gccgggattt gttgtctatt cctccccggg gggcgataaa tgttcatgct
     6541 tccctccttc cttcctttag agggccagac cccatccgct ctgctcttct tcagggagag
     6601 aaagaaacgg gggttaccat tatgctcatg gatgaaggag tagataccgg tcctatattg
     6661 gctcagaaga aagtggaaat agagagagag gataactatc aatctttaac tgagaagttg
     6721 gggaaagagg gaggaaagct tttagtagaa accttacccc gctgggcaag aggggaaatt
     6781 gcccctcgtc ctcaagaggg agaagtttct tatgctccta tattgaaaaa ggaaaaagcc
     6841 aggataaact gggaagatag cgctgttaac atagtgaatc aggttcgagc tttctctcct
     6901 tacccggggg cttatactta tttccaagat aggagaatta aaattttaaa agctaattca
     6961 aaagagggag tttctgctcc tccgggaaca gtggtggaaa ttaatcctca gggatttgta
     7021 gtggctgcgg gagagggagg agtgttagtt gaggaagtac aaccggagag ccgacaagtt
     7081 atgacttctc gagagttttg ttgtggatac catttgagag aaggagaaca attactgtag
     7141 gaggtgaacg atatgttctt tgattacacg tttattcttt taattcctgc actcattctt
     7201 gctttttatg cgcaggctaa agtgagtagt gcttattcta agctatctaa aaagagagcc
     7261 agtgcaggat tgactggcag agaagtagcg gagatgctgg tggagagttt aggattaaga
     7321 gttaaagtgg aggaaatacc aggagcgtta actgaccatt atgaccccac tcgagaggta
     7381 ttgcgtctct cacaggcggt ggccaatggt tcatctattg ctgattacag cattgtagct
     7441 cacgaggtgg gacatgttct gcaaaagagg gaacaatatc ccgcttttgc tttccgctct
     7501 gcactcgttc cggtggcgag ctttggttca caattagctt tgcctctctt tttgattggt
     7561 ttgatttttt atcttcccgc tcttatgaat attggcatta tctttttcag tctggcagtt
     7621 ctttttcagc tggtaacttt accggtggaa tacaatgcca gttccagggc atatcttgtc
     7681 ttagataaag caggattggt aacggcggaa gaaaaacctt atattaaaca gatgttgaat
     7741 gctgctgctc ttacctatgt ggctgctact gcgatggctg cactgcaact cttgcgattg
     7801 gtgctcctgc gggggagcag ggattgagtg attgattacc gaagaagaga tgccctgcag
     7861 attctcaacc gtctttacac cggtcgagtt tcttttttag atgccgaata cagtcgatgg
     7921 attaaaaaga gaaattatac tgaaaaagac cggggctggc tggttagtct attgcgaaac
     7981 atagtaaagc atcaatcgtt tatagatgga gtgcttaagg agtatttacc caggtgggga
     8041 aagcttccta tggctatcca aaacctcttg cgtatagggg tggcagagct tctgttccat
     8101 tcttctccct cttttgctgt agtgaacgaa gtggtggaaa ttacaaaaga cacaggagcg
     8161 gaatttactg ccctggttaa cgctgttctg cggaaaatag cgagaaagag agactattat
     8221 tctgctattt tggaagagga agggatggag ctccctcctt ttttagaaga agagtggaaa
     8281 agctggatag gggaagagga cttaaaattg ctaaaaaaat ctttatctct tcctcctcct
     8341 ctctatgtgc gtgtgaatac cttgaaaatt ggagaagatg agctgaggga aagatggaga
     8401 gaaaaaggaa tagaaagcag taaaactgtg cttccggggg ctttggaggt ggatgctgat
     8461 tactctactc ttctggccct tcctgagtat caggaagggc tgttttaccc tcaggatttg
     8521 ggttctcagc tggtggtcaa aaccatcagc ccctttatcc ggggaagaat aatagatgtc
     8581 tgctgcggaa cagggggaaa ggctttaact atggctcaat acttatccgg gggagaggtt
     8641 tttgcctggg ataaaaaaga gggcaaggtg aggactcttt ctaaatttat ttctctattt
     8701 aatcttggta aaattaaacc tatggtggtg gatactcttc gccctccccg ggaatttttt
     8761 tccactgctg atctggtgct ggtggatgct ccttgttcag ggtggggaac tatccgtcga
     8821 aaccccgaaa tattattgaa aaacccggat ttagaagatt tatctcgtca gcaattttct
     8881 cttcttcttt ctgcttcttc tttggtaaaa aaaggaggag taatggtata ctgtgtttgc
     8941 agtattacta aaaaagaaac tcagaaagta gtaaaagagt gggaattaaa tgcgggaaaa
     9001 gagtgggaaa aagtgggttt gggaaaagga gaagaggaaa tgataatctg gcctcaccaa
     9061 ttcaatagtg atggattttt tgttgcagcc tggagaaaaa aatgaaggag acaaaaagag
     9121 agaactggag ttcttttata cttagagttt ttgcttacgc tcttattttt ggtgggggat
     9181 tagcgctcag tgtttattta ggcttgattt tttttcaata ttatcttaca agcggtacta
     9241 tatccttacc tgattttcgc aaccagagcc tggtagaggc tatcaatcag atatcccagc
     9301 ttggtttaca ggcagagata aagagaatag aaaataatcc ggaatttcct tccagctggg
     9361 ttattcaaca agaccctccc ccgggagtag aagtaaaaaa gaatcatcgg gtaaaattgg
     9421 taatcaacgg aggagatata atagcagaag gaggaagtgt agctaccggt ggctcttctt
     9481 ccccttctac ctctacttct gtttccgctt ctggttctgt ttctgttccc gatgttcggc
     9541 agatggggtt agaagaagca caaagtctta ttgaaagtag tggcttgaag ctagagaggg
     9601 tggcagaaat aacacatgat agcattcctg caggttatgt agtttctcaa aatcctcctc
     9661 cagaaagcga agtggcgaaa ggtagtgcgg taagccttct cgtgagcaaa gggaagggcg
     9721 aagctgaaga gtttaattta gttgttgttc cggatctggt aggactgcgc ctcgaggaag
     9781 ccaaaagatt actggctcag agcaacctca ccgtgggaaa cgtggaagaa atagcggtaa
     9841 aaggaaaaga cgatggcgta gtgatgagac agagccctgc tccgggagaa gaggttgaac
     9901 cgggagaaaa aattcatctt caggtgacta aagctgaaga agaggaagca ggattgaaag
     9961 agctaagttt aaggtttgtt ttacccagtt ctcgaagccc cattaaagtt gaggtggtgg
    10021 taactgatga gctgggtgag cgagtggttt atgaaagaga gcatcaaggg gaagaaatgg
    10081 tggagttaac cactaccaca aaaggtgaag gtaaagtagt tatttacctt aatggttatt
    10141 actactggga taaaactctt taattgatat gaggaggaaa attcatggct aaactggctc
    10201 cttctattct atcttgtgat ttctctcaac tccgggaaga gttgttagct gccgaggagg
    10261 gaggagcaga aataattcat gtagatgtaa tggatggaca ttttgttcct aactttactt
    10321 ttggacttcc tattttgaaa gcggtgagaa aaattttacc ccgggtgact atagatgtcc
    10381 atcttatgac cgataaccct cgtctttaca ttgctgattt tgttcgggag ggagcagata
    10441 tgattagctt ccatttagaa gcatcagccg attatagctt gatagtagac gagattgtag
    10501 aatatggggg taaacccggg gtagcactgg ctcccggaac tccctggcag tgtttaagtc
    10561 ccattctgga taagctttat tatgtgcatc tgatgaccgt tcatcctggt tttggggggc
    10621 agaagtttat cccgggtatg gaagataaaa tcgcttctct tcgccaggag ataagaagaa
    10681 gaggtttatc taccgaaatt gaaattgatg gaggagtaaa tgaccagaac attgagtatc
    10741 tggcttcttg tggtgcctcc attatagcgg cgggttcttt agtttttgct catcaggaaa
    10801 acattgctgc tcgggtgagg atgctttcag aaaagataag gccatactga ctataagggg
    10861 gaactaatcc ccctataacc cccaaaagaa agggaaagag agcaaaaata atacggagga
    10921 aaaacactcc gtgttgtcat tcctgaatgg ttctatcagg aatccatatt atttaagggc
    10981 aaaagtctct ggattcccga caaaagattt cgggaataat agacctcttc ctcgattcct
    11041 tttttctttc cctcaccctt
//
LOCUS       DAOPSS010000049        11042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_4977_length_11042_cov_9.085192, whole genome shotgun sequence.
ACCESSION   DAOPSS010000049 DAOPSS010000000
VERSION     DAOPSS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11042
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4977_length_11042_cov_9.085192"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..589
                     /locus_tag="PKN31_04330"
     CDS             293..589
                     /locus_tag="PKN31_04330"
                     /inference="COORDINATES: protein motif:HMM:NF021402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HOA99186.1"
                     /translation="MKHRARSSKIKYEHTMIPGLREFLEKEIEPLDFVKSIFPGEIKR
                     TRGTTSGLVVRFKYLTPTGAKLLAQKSSAIQEIFIVTNEPETLKKIIINHQGTN"
     gene            623..1573
                     /locus_tag="PKN31_04335"
     CDS             623..1573
                     /locus_tag="PKN31_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99187.1"
                     /translation="MNASTIPDLDTIRKWIDDYAKRVANLQDEKGKGVELLFLRDAIH
                     DGLLYYRSRGAFIDPEETKLSNFDALLDKNAALFLKIAGKKTLKTERDKISPSPEKWW
                     WWLDEKVRERKSRKIKKTITTAGIVVAVLLVLYFTFFRLPPAEQSYLDALTQAERLIY
                     EGEVDEALENIQKAINIFPDRPTPYIMAGCLKEMIGENEEAQKFFTQAQGLYPNEVNF
                     LIEKSNWYFRLNLLERAYSSIIEALQKEPENLAALNLLGSIYEAENKIPEALKVYTKV
                     LELAEEQGAETMIPIARIKIGMLQFRLPLDIPTSFPEEEQ"
     gene            complement(1578..1964)
                     /locus_tag="PKN31_04340"
     CDS             complement(1578..1964)
                     /locus_tag="PKN31_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOA99188.1"
                     /translation="MKLKEQKSEEFLELICIVCPLGCSLSVVKDNDCFQVKGGCKKGE
                     RYAQEEMTNPRRVVTTTLRIKNGVWRRIPVRTSSPFPRDKIPELMRFLNSLEVEAPQK
                     RGDIMVKNLLGEGVDLVVTRTIERRE"
     gene            complement(1927..3183)
                     /locus_tag="PKN31_04345"
     CDS             complement(1927..3183)
                     /locus_tag="PKN31_04345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOA99189.1"
                     /translation="MPHSIKGDVVVIGGGPAGMAAALSSWEAGARSVFLLERNEYLGG
                     ILPQCIHPGFGLQIWGEELTGPEYAETFIEKLNRTGVEVMTDTTVLELSPERVVKAVN
                     SEGVWELKADSIILSMGCRERTRGMLRIPGHRPAGIFTAGLAQYLVNIAGYLPGEEIV
                     ILGSGDIGLIMARRLTLEGARVKMVVEIKNSLSGLVRNYVQCIQDFNIPLLFSHTVTE
                     VRGKERVEAVMISEVDEGGNPLSGSEREVECDTLLLSVGLIPENELSRQAKIPLSPLT
                     GGPWVDEDFSTDLEGFFACGNVVSVFDLVDYVTLVAERAGRAAVFPSSPQKKYILQTG
                     EGVHTVIPQRIREGKDGFLFIRPQKSMRSVTVFLRWEEKIFYEKKISICRPSEMIKLN
                     LSEIPWPQVKGEIKVEIEGTEIRGIS"
     gene            complement(3167..4618)
                     /locus_tag="PKN31_04350"
     CDS             complement(3167..4618)
                     /locus_tag="PKN31_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOA99190.1"
                     /translation="MSEKFDVIVIGGGVVGCAIAWYLAHFDLQILLLEKDLDVCSGVS
                     KANTGIIHSRSYWTPRTLKGELHLRSLPWIEQAEKELDIEFLKTGALTVAFSRSELEF
                     LKTLKRRGKIKGAEILTPQEARTLEPMLSDKIFAAYYDPETRVASPFQLNLALAESAF
                     LNGVRFIFGQEVTGFEKEGNHIRIFTREGEYLSRWVVSSAGLSSSYLAQRSGENIPSL
                     RALRGEYLVLDRECKDLVRHVLYPVPVGETKGILVSPTTDGNILVGPNLEVAEDEDTS
                     TTKEGLKEVEEGGKKLIPFLPVSQTIATFSGLRPTSPDRDFHIFFSSRFPSLLHLVGI
                     ESPGLTASFGIAEYTGDLLKERGLKLSEKRDFIFRSSFPVFRKLSWEERKKLIQEDAD
                     WGKIVCRCEEVTIAEVKHALLSSPGASTLDGLKRRVRVMMGRCQGGFCGMHLPFLMAE
                     TLGLEEREIVKSGKNSFYLGKETKEVRSGAPLY"
     gene            complement(4611..6047)
                     /locus_tag="PKN31_04355"
     CDS             complement(4611..6047)
                     /locus_tag="PKN31_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOA99191.1"
                     /translation="MGNFFLTVDLGTTSVKLIWWSEKGEKVGEDSFPVELRFPFEGGV
                     EFDPEIMVKELVKRINQSPFPLRGMGITNQRETTLVWDEAGKLVYPAIVWQDRRTATW
                     CEAHREKEEWLFQKTGLFLDPYFSLSKLVWILEKVKISRPLHFGTLDTYILWVLSGGK
                     IFSTDYTNASRTLIFNLRSLCWDEEIIKEFGLEKVLFPQVVPSFHLRGETQITASRIP
                     VYVVLGDQQASFLGQGCFERGSTKNTYGTGCFLMANWGNDWPEDTQRLLVTLASLEED
                     KPIFALEASTFSAGVIIEWLKRIELIKSSQDIERMAESARPLEELLLVPAFCGLGAPY
                     WDPFARGAILGLTPQVGKEELVRGALEAVAFEVGDLIRIIDGISPEGIREISVDGGMS
                     QSRYLLQFQADLSGKIIRVFPEKEATSLGVFYLWANRSGFLSREFIEERKQKTLIFEP
                     RGKEKEREKLWKKWQRGIDKVRGWAKNE"
     gene            complement(6051..6632)
                     /locus_tag="PKN31_04360"
     CDS             complement(6051..6632)
                     /locus_tag="PKN31_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HOA99192.1"
                     /translation="MEDLLDIWHKFPVIGALRTSLWKDINGEELSLCPVFFFLEGNIF
                     TLREALKILPSDLHLFLHLDLFGGITSDESGLLFIRETFPQIRGIISTRARTLNLAKK
                     AGFLTIFRLFLIDSESLRTGLRIAQEISPDALEILPGIIFSQVRDFIPVEGLPPLICG
                     GFIRKEKEVEEIISQGAVAVSTSCRKLWKMNRR"
     gene            complement(6729..6893)
                     /locus_tag="PKN31_04365"
     CDS             complement(6729..6893)
                     /locus_tag="PKN31_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99193.1"
                     /translation="MRLLTTMVALVLISPFLYLALLENYPHSFWTKNFIWIYLFLLLI
                     VFLSNLQNRS"
     gene            complement(6906..7715)
                     /gene="hisJ"
                     /locus_tag="PKN31_04370"
     CDS             complement(6906..7715)
                     /gene="hisJ"
                     /locus_tag="PKN31_04370"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HOA99194.1"
                     /translation="MFFGDYHIHTPRCGDAQGSYDEYIEEALRKGLSEIGFSGHCPQY
                     FLPLEERGRESAIPEEELTLYVREVESLQEKYRSSITIRLGLEVDFVPGKEEELQSIV
                     DFYSWDYLLLSVHYLGTWPFDNPQYLSGYRERDINEIYETYYQTLIAGMKTGFFSAVA
                     HFDLPKKFGFRPAGEVKGEIEALQVCREQNLVIELNTAGRRKPVREFYPAAPILAQAQ
                     KLGLNVCLGSDAHRPEEVGEDFREALALLCRLGFDHIAGWEKGIAVLHPIS"
     gene            complement(7776..8066)
                     /locus_tag="PKN31_04375"
     CDS             complement(7776..8066)
                     /locus_tag="PKN31_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOA99195.1"
                     /translation="MNKQEFVNALSEELKKEGVDVTKKDTAKIVDAFLGVIQEEIKRG
                     GKVQLVGFGTFGVKKRAARRGRNPQTGKEIHIPEATVPFFRPGKGLKELVKE"
     gene            complement(8108..8368)
                     /locus_tag="PKN31_04380"
     CDS             complement(8108..8368)
                     /locus_tag="PKN31_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOA99196.1"
                     /translation="MELLKISSKTRPVSLAGALTGVIREQGRAEMQAVGAGAVNQAVK
                     AIAIARGYLAPSGIDLVCIPAFVDVKIDDTEKTAIRFIVLPA"
     gene            complement(8583..9863)
                     /locus_tag="PKN31_04385"
     CDS             complement(8583..9863)
                     /locus_tag="PKN31_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOA99197.1"
                     /translation="MKAQPECFCCSIRQAQEATEIAGRDKDTVWKVSQEVCAVYSRAD
                     RNWTPAYMTTLAHDVTKKIVGIEDIFYQLKREHNKAALQFYPQLKEWVKRGDNSLERA
                     VKVAIAGNIIDLGVYREVRVEEVLEEVNHAQWGRYEFASWVEDLKNAHTLIYVGDNAG
                     EIVFDRILLEEISPGRKVFFVVKGGPISNDALLEDAQEAGIDEMVEILTTGQAEVGVA
                     LERAPSSLQKLWKEADMIIAKGQGNFETLSEEEGNIYFLLKAKCVPVARELQVKQGDL
                     VLQKRKEKISSSEVKVYSSGGLVVRKNNGEWQILLVKKSDLGLWTLPKGHKEKEEREE
                     ETACREVEEETGYKVKLGPRVGDISFSYVRDHQVFREIASFFLMEVVGKGEKEQEGEI
                     EEAKWFSLEEALQVLSYDNEKSLIGKALELLERR"
     gene            complement(9860..10546)
                     /locus_tag="PKN31_04390"
     CDS             complement(9860..10546)
                     /locus_tag="PKN31_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOA99198.1"
                     /translation="MRQFAVFGLGIFGSSIAIALHEQGFIVLGVDIEEEPVKELAGKI
                     TEVVQADTTDEKVLEALGVRNFDVAIVSIGNDIQSSVLTTLAVKELGIPLVISRAINE
                     MHGKILHKIGADRVIFPEQDTAIRVAKTLAFPYAIRSEELFPGHNIIESQVPPSLYGF
                     TLAKAQLRTRYGITVIAIKRGENYIVSPNATESLSKGDIIYILGSEQQLKKFFKEMAE
                     IRNGKVKVEE"
     gene            complement(10561..>11042)
                     /locus_tag="PKN31_04395"
     CDS             complement(10561..>11042)
                     /locus_tag="PKN31_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOA99199.1"
                     /translation="LLCPFKLGLIQTWQEEKKPMKFNTLRVGLTGVAQVVVGDENAAD
                     HFAPDMVPAFATPMLLSLMDNAAHEAVKNHLPEGYVSVGTRLSISHVAATPKGMQVTA
                     RATLVEIHRNRLIFEAEAFDEVEKVGEGRLERFVVNRDSFLKKLESKLDFIKGLTTE"
BASE COUNT         3333 a         2772 c         1811 g         3126 t
ORIGIN      
        1 actatggtat aaatgtgtct cgctttacgg ttttcagcta taactttcat tatttagata
       61 acccagtcat cctcaaaaaa ttcccttgcc agagtaagta tagcacaatt tatccttcta
      121 ttatagtatc cccaaacctg tccatgaatt ttgcggttaa ctatgaattg acttttaaga
      181 ctaattaaaa acttaagaaa tcgggttgaa agagaataag ttttgaataa aagttgatta
      241 cttctcacaa acagctaaac tattaagaga aattataaaa aaagagttat ctatgaaaca
      301 tcgagccaga agttcaaaaa ttaaatacga acacactatg ataccaggct tgagagaatt
      361 cttagaaaaa gagatcgagc ctctggattt tgttaaatct attttcccgg gagaaataaa
      421 acgcaccaga ggaacaacat caggccttgt ggtacgcttc aaatatctta ctccaacggg
      481 ggcaaagctt ttagctcaaa agtcctcagc cattcaagaa atatttatag taaccaatga
      541 gcccgagact ttgaaaaaga taattattaa ccaccaggga actaattaga tgaaattttt
      601 ataatctact gcagagatga gtatgaatgc ttcaactatt cctgatttag ataccattag
      661 aaaatggatc gatgactatg ctaaaagagt ggctaacctc caggatgaga aaggaaaagg
      721 agtagagctc cttttccttc gagatgccat ccatgatggg ctattatact atcgctctcg
      781 aggagcattc attgacccag aagaaactaa attaagcaat tttgatgctc ttttagacaa
      841 aaatgctgct ctctttctca aaatagcagg aaaaaagact ctcaaaacag aaagagataa
      901 aatctctcct tctccagaga aatggtggtg gtggttagat gaaaaggtga gggagagaaa
      961 atcccgcaaa atcaaaaaga ctataaccac cgcagggata gtggttgctg ttcttctggt
     1021 tctttatttc accttttttc gccttcctcc tgcagaacaa agttatttag acgctcttac
     1081 ccaggcagaa aggctaatat atgaaggcga agtggacgaa gccttggaaa atatccagaa
     1141 agctataaac atttttcccg accgccctac tccttacatt atggctggtt gcttaaaaga
     1201 gatgatagga gaaaacgaag aggcgcaaaa gttttttacc caggctcaag gtctttatcc
     1261 caatgaagta aattttctaa tagaaaaaag caactggtat tttagactta atctcttaga
     1321 gagagcttac tcttccatta tagaagcttt acaaaaagaa ccagaaaatc ttgctgctct
     1381 caacctctta ggctcaattt atgaagcgga aaacaaaatt cctgaggcct taaaagtgta
     1441 cactaaggtt ttagaactgg ccgaagaaca aggggcagaa accatgattc ccattgctcg
     1501 aataaaaatt ggcatgttgc agttccgcct accactcgac atccctactt cttttccaga
     1561 agaagaacag taagtaacta ctctcttcgc tctatagttc tcgttactac caaatccact
     1621 ccttcaccca gcaaattttt taccattata tctcctctct tttgaggagc ttctacctct
     1681 aaagaattca aaaatctcat aagctcagga attttatccc ggggaaaagg tgaagaagtc
     1741 cgtactggta ttctccgcca cactccgttt ttgattcgaa gagtagtggt aaccactcgc
     1801 cgaggattgg tcatctcctc ctgcgcatat ctctctccct tcttacaccc cccctttacc
     1861 tggaaacaat cgttatcttt caccaccgat aaactacaac ctaaaggaca aacaatacaa
     1921 ataagttcaa gaaattcctc ggatttctgt tccttcaatt tcaactttaa tctctccttt
     1981 tacctgtggc cagggtattt ccgaaaggtt caattttatc atttcactgg gccggcaaat
     2041 cgatatcttc ttttcgtaga atatcttttc ttcccatcga agaaataccg ttacacttct
     2101 catgctcttt tgcggccgaa taaataaaaa accgtctttc ccttccctta ttcgctgggg
     2161 aataactgta tgcacccctt ctcctgtttg taaaatatat tttttttgag gagaggacgg
     2221 aaaaactgct gcccgaccgg ctctttctgc caccaaagta acataatcca cgagatcgaa
     2281 gacagaaact acattaccac aagcaaaaaa gccttctaaa tcggtagaaa aatcttcatc
     2341 cacccaggga cctccggtta agggagaaag gggaattttt gcctgacgag aaagttcatt
     2401 ttccggaatt aatcccacag agagtaaaag agtatcacac tctacttccc tctcactgcc
     2461 tgagagaggg tttccacctt catccacttc actaatcatc accgcttcta ctctttcctt
     2521 gcctctcacc tcagttaccg tatgggaaaa aagaagagga atattgaaat cttgaataca
     2581 ttgaacataa ttgcgaacca ggccactcaa tgaattcttt atctctacta ccatttttac
     2641 ccgtgctcct tccaaagtaa ggcggcgagc cataatgaga ccaatgtccc ctgaacccaa
     2701 aatcactatc tcctcacccg gtaaataacc agcaatgttt acgagatatt gagctaatcc
     2761 agcagtaaaa attcctgccg ggcgatgacc aggaatccgc aacattcccc gagttctttc
     2821 ccgacaaccc atgctcaaga taatcgaatc agcctttagc tcccaaaccc cctcggagtt
     2881 caccgcttta accacccgct cgggggatag ctccaaaaca gtagtatcag tcatcacttc
     2941 tacacctgtt ctatttaatt tttctataaa tgtttctgca tactcaggtc ccgtaagctc
     3001 ttctccccat atttgcaagc caaaaccagg atgaatacat tgaggaagaa ttcctcccaa
     3061 atactcattt ctctccagaa gaaaaactga ccttgctcct gcttcccagg aagataaagc
     3121 agcagccata ccggctggtc ctcctcctat tactaccaca tctcctttaa tagagtgggg
     3181 caccacttct cacctctttg gtttctttac ctaagtaaaa agagttttta cccgatttga
     3241 caatttctct ctcttccaat cctaatgttt ccgccattaa aaatggcaaa tgcataccac
     3301 aaaatccccc ctggcatctt cccatcatta ctcgaactct ccttttcaaa ccatccaggg
     3361 tagaagcccc cggcgaagat aaaagagcat gtttaacctc tgctattgtt acttcctcac
     3421 agcgacaaac tatttttccc caatcagcat cttcttgaat aagttttttc cgctcctccc
     3481 aggaaagttt ccgaaaaact ggaaaggaag agcgaaagat gaaatccctt ttctcactca
     3541 gctttaatcc tctctcttta agtaaatccc ctgtatattc tgctatacca aaagaagcag
     3601 taagccccgg agattcaata cccacaagat gaagtaaaga gggaaaacga gaggagaaga
     3661 aaatatggaa atcccgatct ggcgaagtag ggcgcaatcc agaaaaagtg gcaatagtct
     3721 gggaaacagg taggaaagga atcagttttt tccctccctc ctctacttct tttaatccct
     3781 cttttgtggt ggaagtatct tcatcctctg ccacctcaag gttaggccct actaaaatat
     3841 taccatcagt ggtaggggaa accagaattc ctttagtctc tcccaccgga acaggataaa
     3901 gaacatgacg aactaaatcc ttacattccc gatccaaaac caggtattct ccccggagag
     3961 ctcgaagaga aggaatattt tctccacttc tttgagctaa ataagaagaa gatagccccg
     4021 cagaacttac cacccagcga gataaatatt ctccctccct ggtaaatatc cttatatggt
     4081 ttccctcttt ttcaaaaccg gttacctcct ggccaaaaat aaaacgcacc ccattcagaa
     4141 aagcactttc tgccagagcc aaattaagct gaaaaggaga ggccactcga gtctcagggt
     4201 cataataagc agcaaatatt ttatcgctta gcatcggctc taaagtacgc gcttcttgag
     4261 gcgttaaaat ttctgctccc tttatttttc ctctcctttt tagagttttt aaaaattcaa
     4321 gttcgcttcg agaaaaagcc acagtcagag ctccggtttt taagaactcg atatccagtt
     4381 ctttttccgc ttgctcaatc cagggaagag aacgcagatg tagctctccc tttagagtac
     4441 ggggagtcca gtagctacgg gagtggatta ttccggtgtt agctttactc actcccgaac
     4501 agacgtctaa atctttttct aaaagaagaa tctgaaggtc gaaatgggct aaataccagg
     4561 ctatggcaca acccaccact cctcctccga ttacaattac atcgaacttt tcactcattc
     4621 ttagcccaac cccttacctt atctattcct ctctgccatt tcttccatag tttttccctt
     4681 tctttttctt tccctcgagg ttcaaaaatc agagtctttt gtttcctttc ttcaataaat
     4741 tcccgagata aaaaacctga tcggttagcc caaagataga aaacccccag tgaagtagct
     4801 tctttctctg gaaaaactcg gataattttt ccactcaaat cggcctgaaa ctgcaaaaga
     4861 tatctgctct ggctcatacc tccgtctacg gatatttccc ttattccctc tggagaaatc
     4921 ccatctatta tccgtataag gtctcccacc tcaaaagcca cagcttccag cgctccccgc
     4981 actaattcct ctttacccac ctgaggagtt aatcccagaa tagcccctcg agcaaaagga
     5041 tcccagtagg gagcacccag accacaaaaa gcaggaacga gaagaagctc ttctaaggga
     5101 cgagcacttt ctgccatcct ttctatatct tgagaacttt taatcaattc tattctcttt
     5161 agccactcta taattacccc agcgctgaaa gtgctggctt ccagggcaaa gataggttta
     5221 tcttcttcta aagacgctaa agtaaccaaa agcctctgag tatcttctgg ccaatcgttt
     5281 ccccagttag ccatcaagaa gcaacccgtt ccataggtgt ttttagtact ccccctctca
     5341 aaacacccct ggcctaagaa agaagcctgc tgatcaccta aaaccacata aacaggaatg
     5401 cgagaagcgg taatctgcgt ttccccccgg aggtgaaaag aaggcaccac ctgaggaaaa
     5461 agaacttttt ctaaaccgaa ctcttttatt atttcttcat cccagcacaa actgcgaagg
     5521 ttaaaaataa gagtgcggga agcgttggta taatcggtag agaaaatttt tcccccggat
     5581 aaaacccaca aaatgtaggt atctaaagtg ccaaaatgaa gagggcggga aatttttacc
     5641 ttttctaaaa tccacaccaa tttactgaga gaaaagtaag ggtcgagaaa aagaccagtt
     5701 ttttgaaaaa gccattcttc tttctcccga tgagcttcac accaggtagc agtgcgacga
     5761 tcctgccaca ctatagcagg ataaaccagc ttccctgctt catcccacac taaggtagtc
     5821 tctctctgat tagttatacc catacctcga agaggaaaag gcgattgatt tattcttttt
     5881 accaattctt ttaccattat ctccggatca aattccaccc ctccttcaaa aggaaaacga
     5941 agttctaccg gaaaggaatc ttcaccaact ttctctccct tttcactcca ccatatcaac
     6001 ttcacgctgg tagtccccaa atctacagtg agaaaaaaat ttcccatttt ctacctcctg
     6061 ttcattttcc acaacttccg acaactggta gaaaccgcaa ctgccccctg ggatattatt
     6121 tcttccacct ctttctcctt ccggataaat cctccacaaa taagaggagg taaaccttct
     6181 acaggaataa aatccctcac ctgagagaaa ataatcccgg gcaatatttc cagggcatcg
     6241 ggagaaatct cctgcgctat ccggagacca gtacgcagag attcactatc gatgagaaat
     6301 aatcgaaaaa tagtaagaaa ccccgctttc ttagctaaat ttaaggtacg agctcgggta
     6361 gagataatgc ctcggatctg aggaaaagtt tctcgtataa agagcaaccc gctctcatca
     6421 gaagttatcc ccccaaaaag atctaaatgc agaaagagat gtaaatcaga aggtaaaatt
     6481 ttcaacgcct ctcgcaaagt aaatatattc ccctccagaa agaaaaagac tggacacaaa
     6541 gaaagctcct caccattgat atctttccag agagaagtcc gcagagctcc aattaccggg
     6601 aacttatgcc agatatctaa taaatcttcc atatgtacct cctaccaaca aaaaagcctt
     6661 ccgagtaaag caatattact cggaaggctt tttctccttt tctcctcgcc attcctattt
     6721 taacacaatc aactcctatt ttgaagatta gaaagaaaaa caatcaggag cagaaaaaga
     6781 tagatccaga taaagttttt agtccagaaa gaatgagggt aattttctaa aagagctaaa
     6841 tataaaaagg gagaaatcaa aaccagagcc accatagtag tgagcagcct caactttttt
     6901 actcctcaag agataggatg taaaactgca atcccttttt cccagcctgc tatatgatca
     6961 aatcctaagc gacacaaaag tgcaagagct tctctaaaat cttcccccac ttcttccggt
     7021 cggtgagcat cagaaccaag acacacattc agacccagct tttgcgcttg tgcaagaata
     7081 ggggctgcag gataaaattc ccttactggt ttgcgtcgac cagcagtgtt aagctctatc
     7141 accaaatttt gctcccgaca aacttgcaga gcctctatct ctcccttaac ttcaccagca
     7201 ggtcgaaaac caaacttttt aggcaaatca aaatgagcaa cagcgctaaa aaaaccggtt
     7261 ttcataccgg caatgagggt ttggtaataa gtttcataaa tttcgtttat atctctctcc
     7321 ctgtaaccag aaaggtattg aggattatca aaaggccacg ttccaagata atgaacagaa
     7381 agcaaaagat aatcccagga ataaaaatca actatggatt gtaattcttc ttcttttcct
     7441 ggaacaaaat ctacttctaa ccccaagcgg atggtaatag aagaccgata cttttcctgt
     7501 agagattcca cctcccggac atagagagtt aattcctctt ccggaatagc tgattctctt
     7561 cccctttctt ctaaaggaag aaagtactga gggcaatggc cagaaaaacc aatctcactg
     7621 agcccttttc tcaaagcctc ttcaatatac tcatcatagc taccctgtgc atctccacag
     7681 cgaggagtgt gaatatgata atcaccaaaa aacatttttc tcctaaaaac aaaaaaggag
     7741 ggactgtttg tccctcctta agtttaatac tatttttatt ccttaaccag ctctttaagg
     7801 ccttttcccg gacggaaaaa gggaaccgta gcttcaggaa tatggatttc cttaccggtc
     7861 tgcggattcc ttcccctcct tgccgctctc tttttcactc caaaggtccc aaaccccact
     7921 aactgcactt taccgcccct tttaatttct tcctgaatca ctcctaaaaa cgcatccaca
     7981 atctttgccg tatctttctt ggtaacatcc accccttcct ttttcaactc ctcagaaagg
     8041 gcattgacaa attcctgctt attcatactt tccttacctc cttattattt taatttatta
     8101 tttcccctca agcggggagt actataaacc tgatagcagt tttttccgta tcatcgattt
     8161 tcacatccac aaaagcagga atacaaacca gatcgatacc actgggagct aaatagcccc
     8221 gggcaatagc aatggccttt actgcctgat tcactgctcc tgcccccact gcctgcatct
     8281 cagctctacc ctgctccctg attactccgg ttaacgctcc agctaaagac accggcctag
     8341 ttttagaaga tattttcaag agctccatat ttaagcctcc ttagcgttta agaaatgccg
     8401 tttttaaaaa gaaaatctct ttctatttct ggaggatacc tttatccctc cttatactcc
     8461 attttaacgc attttgcaag agctgaacag cctacagacc aaaaatttcg caaatttttt
     8521 ataataatag ccttaatagc tatttttcag aaagagaatg cagaaagaaa ggacagaatc
     8581 acttacctcc tctccaaaag ctccagagct ttaccaatta gagatttttc attatcatag
     8641 ctcaagactt gtagagcctc ttccagagag aaccatttcg cttcttcaat ttctccttct
     8701 tgctcttttt ccccttttcc taccacttcc attaggaaga aagaggctat ttctcgaaaa
     8761 acctgatgat ctctaacata gctgaagcta atatccccca ctcgaggtcc taatttgact
     8821 ttatagccgg tttcttcctc cacttctcga caggcggttt cttcttctct ttcttctttt
     8881 tccttatgac ctttggggag agtccacaac cccaaatcac tttttttcac taaaagtatt
     8941 tgccactcgc cattattctt tcttaccacc aaccccccac tagaatacac cttaacttca
     9001 gaagaagaaa ttttttcttt tcttttttgt aaaactaaat ctccttgctt tacctgtaac
     9061 tcccgagcca caggaacgca cttggccttg agcaagaaat aaatatttcc ttcttcttca
     9121 ctcaaagtct caaaattacc ctgtccttta gcaataatca tatctgcttc tttccacaat
     9181 ttctgaagag aagagggtgc tctttctaaa gctactccca cctctgcctg accggtggtt
     9241 agaatttcta ccatctcatc gatacctgcc tcttgagcat cctctaaaag agcatcatta
     9301 gatatggggc cacctttcac cacgaaaaat acctttcttc ccggagatat ctcttccaga
     9361 agaatccgat caaaaactat ttctccagcg ttatctccca cataaatcag agtatgagca
     9421 tttttcaaat cttctaccca ggaggcaaat tcatatcttc cccactgagc atgattaacc
     9481 tcttccaaaa cttcttctac tcttacttcc cggtaaaccc ccagatcaat tatgtttcca
     9541 gctatagcta ccttcactgc tcgttctaaa ctattatctc ctcttttaac ccattcttta
     9601 agctgaggat aaaactggag tgctgctttg ttatgttctc tcttcaactg ataaaatata
     9661 tcttctattc ccacaatttt tttagtcaca tcgtgagcca aagtagtcat ataagcagga
     9721 gtccaatttc gatcagcccg agaataaacc gcacaaactt cctgagaaac tttccagacg
     9781 gtgtctttat ctcttcctgc tatctcggta gcttcttgag cctgtcttat gctacagcaa
     9841 aaacactctg gttgagcttt cattcttcca cctttacctt tccatttcta atttccgcca
     9901 tctctttaaa aaacttctta agttgctgct cgctacccaa gatgtaaatt atatctcctt
     9961 ttgataagct ttccgtagcg tttggtgata cgatatagtt ttccccacgc tttatggcta
    10021 tcacagtaat accatagcga gttctcaatt gagccttagc taatgtaaac ccataaagag
    10081 aaggaggcac ttgagactca ataatattat gtcctggaaa tagctcttcg cttctaatag
    10141 cataggggaa agccagggtt ttcgccaccc gtatagcggt atcctgctca ggaaatataa
    10201 cccggtcagc accaatttta tgaagaattt tcccatgcat ttcattaata gctcgggata
    10261 tgaccaaggg tattcccaat tctttaactg ccaaagtagt gagtacacta gattgaatgt
    10321 cattaccaat actcactatg gctacgtcaa aatttctcac tcccagggct tccaaaactt
    10381 tttcgtcagt agtatcagcc tgaactactt cggtgatttt acccgctaat tctttaaccg
    10441 gctcttcttc gatatctacc cccaagacaa tgaaaccctg ttcatgaagg gcaatagcaa
    10501 tactacttcc aaagatgccc aggccaaaca cagcaaactg tcgcataaga tctaccccgg
    10561 ctattctgtc gtcaaacctt taataaaatc cagcttagac tctaactttt ttaagaaaga
    10621 atctcgattc actacaaatc tttctaatct tccttctccc actttttcca cctcgtcgaa
    10681 agcctctgcc tcaaagatta agcgattccg gtggatctcc acaagagttg ctcgtgcagt
    10741 aacctgcatg cctttgggag tagcagcaac atgagaaata cttaaacgtg ttcccacgct
    10801 aacataaccc tcaggaaggt gattttttac tgcttcatga gcagcattat ccatcaagga
    10861 gagcaacata ggagtagcaa aagcaggaac catatcggga gcaaaatggt cagcggcatt
    10921 ctcatcaccc accacaacct gagctacccc cgtaagacca actctcaaag tattaaattt
    10981 catcggtttt ttctcctcct gccaggtctg aattaatccg agtttgaagg ggcagagcag
    11041 aa
//
LOCUS       DAOPSS010000050        10975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_5015_length_10975_cov_8.016392, whole genome shotgun sequence.
ACCESSION   DAOPSS010000050 DAOPSS010000000
VERSION     DAOPSS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5015_length_10975_cov_8.016392"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKN31_04400"
     CDS             complement(<1..655)
                     /locus_tag="PKN31_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOA99200.1"
                     /translation="MYLLGTDIGTQGTKTVMVNEKGEFITDAFQEYEVITPRPSWAEQ
                     WPDVWLEAVVKTISECVEKSKVDPREIAGLAISGLYGGSGIPVDEEIKPLYPCLIWMD
                     RRAREETQWVKDHVPQEKIFGITGNYVDSYYGFTKMMWIRNNEPEVWKKIYQFVTPKD
                     YVVYHLTDVLATDYSSAGNIGGVFDIRKRTWSEEMCEILGIPKRMLPDKILKSSDIVG
                     "
     gene            1070..1963
                     /locus_tag="PKN31_04405"
     CDS             1070..1963
                     /locus_tag="PKN31_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOA99201.1"
                     /translation="MLSGILKREVIIFDGAMGTRLQKSGLPAGHPPEEWNLSHPEEIA
                     RVHRSYVEAGSTLIQTNTFGANRIRLERYHLEGKIKEIIHQAAEIAKSTLKEGVLLAA
                     SIGPLGEFIEPYGDLSPEEAQKVFQEQVELLQEEGIKIFHLETFSFSSEALLALEAIK
                     NSGGEAIVSFTFEERGEEHFTTLMGESPHQIVEIFKDESIAALGANCGGGIKEIIEIA
                     RQYRESYPGPLSFKPNAGIPQVVEGEVIYTETPEDFERGTKQLLQLGASIIGGCCGTD
                     EKYIAAIKKAIQEVSAFKPQL"
     gene            complement(1947..2705)
                     /locus_tag="PKN31_04410"
     CDS             complement(1947..2705)
                     /locus_tag="PKN31_04410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA99202.1"
                     /translation="MAKKCIFGPILSRRLGSSLGINLLPYKTCSLNCIYCECGVTTNL
                     TTERKEYIPTQEVIESLKKYAASSQFRSAPPYCITFAGWGEPTLHSALGEIAHFIKKL
                     FPEQKLVLITNGTLFAFYEELSEEVKEMDIIIPSLDAASPQALQAIDQPHPRITLNSY
                     IESLIKLRKHFPGEIWLEIFIVEGINDSERELNFLKEKIALVSPHRVQINALDRNPAR
                     EGVKSPPFYKLEEIASFLGGETILPSPRKSKVVV"
     gene            complement(2721..3266)
                     /locus_tag="PKN31_04415"
     CDS             complement(2721..3266)
                     /locus_tag="PKN31_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOA99203.1"
                     /translation="MLETFIIAIGLGLDAFSLATAFGMCQKACPRDARLRLSFSFGAF
                     QFFMPLVGFGVGRQVARFISSFDHWVVLGILSLVGGKMIYEGWKRDSDTPLIDFSRGW
                     PLFLASLATSIDALAVGFSFALMGKNIWFSALIIGIVALFMTYSGVSLGHKIRGVFSS
                     PEVMGGIAIILVGLKIFLEDL"
     gene            3458..3952
                     /locus_tag="PKN31_04420"
     CDS             3458..3952
                     /locus_tag="PKN31_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOA99204.1"
                     /translation="MMANLSVDEEIISPDQEEALELILQKFKGKPGSLIQALHAAQNL
                     IGYLPPPVLKKVSHVLDIPLSEIYGIVTFYHFFSLKPRGKHIIQVCLGTACYVRGGQE
                     ILNRLEEDLEIGVGEVTEDRLYSLEVMRCAGACGLAPIVKIDNDIYKRVNPAQIMDIV
                     KSYS"
     gene            3957..4517
                     /locus_tag="PKN31_04425"
     CDS             3957..4517
                     /locus_tag="PKN31_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA99205.1"
                     /translation="MKELSLHILDVVENSLRAKAKKVRISILEDTRENRLELVIEDDG
                     EGMDEETLKMVRDPFFSGQGKKVGMGIPLLDQLTRQCEGNLEILSEKGKGTTVRATFQ
                     RDNLDLPPLGKLADTLFVIMASHPEVELEFIHRIDDCQWLFNSKKIEEELDLTEENRG
                     VLLPAIREWIEYQEEILKGGIGNEDK"
     gene            4504..4884
                     /locus_tag="PKN31_04430"
     CDS             4504..4884
                     /locus_tag="PKN31_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOA99206.1"
                     /translation="MKISSIEDLRRIKEEAQKRTLLREGKADYRVVVHMGTCGIAAGA
                     REVMSAILDEIAKRGLTNVMVTQAGCIGLCDREPLVSVEKGEERILYGDITPDRARQI
                     IASHLVNGQIVGDWVVHAEGRARR"
     gene            4890..7976
                     /gene="nuoF"
                     /locus_tag="PKN31_04435"
     CDS             4890..7976
                     /gene="nuoF"
                     /locus_tag="PKN31_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOA99207.1"
                     /translation="MAEQERCQVLLCAGAACISSGALKIKEALVRELQKNALDNEVRL
                     IETGCVGPCNLGPLAIIYPEGVFYQKLTPEDAKEIVEEHLLKGRVVERLLYQPPQEER
                     KKFLREIDFFDKQLKIALRNCGFINPLNIEEYIARDGYLALSRVLTEMTPEEVIAEVK
                     KSGLLGRGGAGFPTGLKWELTRQAKGYPKFIICNADEGDPGAFMDRSILEGDPHSVIE
                     AMTIAGYAVGAEKGYVYVRAEYPLAVERLEYAINQAREYGLLGEDILNSGFNFDVEIR
                     IGAGAFVCGEETALIASVEGKRGMPRPKPPYPAQEGLWGKPTVINNVETYANIPPIIL
                     QGADWFRSIGAENSKGTKVFALAGDINNTGLVEVPMGISLGEVIYDLGGGIRGGKKLK
                     AVQIGGPSGGCIPVGYLNVKIDYDSLKELGAIMGSGGLIVMDEDTCMVDLARFFLEFV
                     QDESCGKCTPCRIGTKRMLEILQRITQGEGREGDIERLEDLANQIKTTALCGLGQTAP
                     NPVLSTIHYFRDEYEAHIREKKCPAVVCRPLFVSPCQHACPLGINIPGYISCIKEGDY
                     LGALQIIREDNPLPSVCGRVCHRLCESKCRRGDMDEPVAIDDLKRFVADYARQNRIAL
                     PVVMDRKRDESVAVVGSGPAGLTCAYYLARLGYSVTIFEALPVAGGMLAAGIPPYRLP
                     REALEYDINQIKAMGVKIELNKAVGKEISWKEVREKFDAVFLGIGAWKSAPLGIPGEN
                     LSGVIAALDFLRDFNLGGYVEVPRRAVVVGGGNAAIDAARTLLRLGAEEVNVVYRRTR
                     GEMPAISEEVEAAEEEGVIFHFLMAPLEVIGENGKVKGIRLQRMKLGEFDRSGRRRPI
                     PVEGAEAFWECDMVVEAVGQIPEVESLGEMLSVDHRGNILVDRYSLSTSLPGVFAGGD
                     AIGEEATVVHAMALGKKAAFSIHQYLRDLKPEEEEVIIRPERPKILEEPPVLQEIPRV
                     KPPELPVRERVCSFSEVKLCLEEEDARREAERCLRCDLEKVLKKYQEIVMAEEEVQ"
     gene            7973..8437
                     /locus_tag="PKN31_04440"
     CDS             7973..8437
                     /locus_tag="PKN31_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOA99208.1"
                     /translation="MTGLAVYEEIDEIIGKHQEEKTPLLSILQEIQERWRYLPGDVLG
                     YVSRKLDVPSSMVWGVATFYSFLKTEPAGEYVIRVCCNAPCHVNGSVDVLKTLQKELG
                     IKEGETSRDGKFTLETTSCLGVCGVAPAMMINDVTYGNLSEDRIREILALYR"
     gene            8448..10082
                     /gene="nuoF"
                     /locus_tag="PKN31_04445"
     CDS             8448..10082
                     /gene="nuoF"
                     /locus_tag="PKN31_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOA99209.1"
                     /translation="MFYRSHVLVEMTSNSLVRGAAQIKERLEREIERLGLDKEIKVLE
                     TGSLGAALPSPFVVVYPDNIVYAPVTVSDVKRIVEEHFWKGRPVGDLIFSPSERREIG
                     VFLPYFPLTKEKRVVLRNSGQIDPTNIEEYIARDGYLALSRVLTEMTPEEVIAEVKKS
                     GLLGRGGAGFPTGLKWEFTKNAEGVEKYIVCNADEGEPGTFKDRLILEGDPHSIIEAM
                     AIAGYAVGAGEGYIYVRGEYPQSINHLRIAIEQAREYGLLGENILGSSFSFEVRIRKG
                     AGAYICGEETALLESIEGKRGIPRNKPPYPAQEGLWGKPTVINNVETLANIPPIILQG
                     ADWFRSIGTPTCTGTKVFTLTGNVNNLGLIEVPMGITLRELIFEVGGGIRGGHNLKLV
                     QTGGPSGGTMTPDLLDVPMAFDTLSRYDSALGSGALTVIDDTHCIVDVVRNFMEFFLY
                     ESCGKCTPCRVGTKRIVEILNRISEGRGEPSDLENLRYMGEHIKSTSFCGLGQAAPNP
                     LLRSLSFFEEEFRAHLEEKRCPVDTCSMKTKTKILI"
     gene            10102..>10975
                     /locus_tag="PKN31_04450"
     CDS             10102..>10975
                     /locus_tag="PKN31_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOA99210.1"
                     /translation="MAKIVEEMKEGTGQKKLVTIYLDGREVKVPEGVSILEACNMEGI
                     KIPTLCYFEGLTPWGGCRICMVEVEGQPNLVASCVTDVREGMKIKTSSKKVREARKTN
                     LELLLSNHPLDCQLCDRNGSCELQDLSYQFGVREIRFPGEKKTYEVDRSSPSIKRDAN
                     RCILCGRCIRACQEIQAVFAIDFANRGFDSLVSPSLGLPLGESVCVNCGQCILACPTG
                     ALSEVSHVEMVWEALEDPEKFVILQTAPAIRVSIGEPFGLPPGTVTTGKMVAAFRRLG
                     FDRVFDTNFAADLTI"
BASE COUNT         3159 a         1949 c         2730 g         3137 t
ORIGIN      
        1 ttcctactat atcagaggat tttaaaattt tatcagggag cattctttta ggaatcccca
       61 aaatttcaca catttcttcc gaccaggtgc gctttcgaat atcaaaaact cctccaatgt
      121 ttcccgctga cgaataatca gtagctaaaa catcggttag atgatagacc acatagtctt
      181 taggagtgac aaactggtag atttttttcc acacttctgg ctcgttatta cgaatccaca
      241 tcattttggt aaagccgtaa taggaatcaa cataatttcc agtaattccg aaaatttttt
      301 cctggggaac atgatctttt acccactgag tttcttctcg tgctcttcta tccatccaga
      361 ttaagcaagg atagagaggt tttatctctt cgtctactgg aatacctgaa cccccataaa
      421 gaccgctgat agctaacccc gcaatctccc gaggatcgac cttggatttt tctacgcact
      481 cagagatagt tttaaccact gcctctaacc atacatcagg ccactgttct gcccaggaag
      541 gtcggggagt gatgacttca tactcttgaa aagcatcagt tataaactca cctttttcgt
      601 tgaccatgac tgttttggtt ccctgtgtac caatatctgt tcctaaaaga tacattttta
      661 ttcacccctt cggctttaat ttctactatt ttaccctacc ttgacgaatt gctcaaaatc
      721 tattttttta aaggggactt ttctttatta cttttcctgt aggagaaaga aggtaaatac
      781 cagacttgta aaaagggaag ttcacttctt tttcgggatt tatagtgttc tgcaaaaccg
      841 gtcatgatag taggggaagc tcccttgtgc tggtgaatga tttttttcac cagtcgtatt
      901 tgctttttac gtttttttga ggtttataag aggatctctt cctcaataat atttgttttt
      961 ttagactttt acgttttttc caggggctta taaggggggg cagcactctc ttatagtctg
     1021 ttctatgata tatttatctt aaatttatct taaaaagagg aggaaaagta tgctgtctgg
     1081 gatattaaaa agagaggtaa taatttttga cggagcaatg ggaactcgct tacagaaatc
     1141 aggtcttccg gcaggccatc ctccagagga atggaacctt tcccatcctg aagagatagc
     1201 ccgggtgcac cgaagctatg tagaggcagg ttctaccctt attcaaacca atacctttgg
     1261 cgccaaccgt attcgtcttg agcgttacca cctggagggg aaaattaagg aaataatcca
     1321 ccaggcagca gaaatagcta aaagtacact gaaagaagga gtgctgctgg ctgcctcaat
     1381 aggacctctg ggagaattta ttgaacctta tggagacctc tctccagaag aagctcaaaa
     1441 agtttttcag gaacaagttg agcttttaca agaagaggga ataaagatat ttcacttaga
     1501 gactttcagt ttttcttccg aagctctttt agccttggag gcaattaaaa attctggtgg
     1561 tgaagccata gttagtttta cctttgagga gcggggcgag gaacatttta ccactttaat
     1621 gggagaaagc ccccatcaaa tagtggaaat ttttaaagat gaatccatcg ctgctctggg
     1681 tgctaactgt ggaggaggga taaaggagat aatagaaatt gcccgtcaat atcgagaaag
     1741 ctatcccggg cctctttctt ttaaacctaa tgctggcatt cctcaagtgg tggaaggaga
     1801 ggtaatttat accgaaactc cggaagattt tgaaagagga actaaacaat tacttcaatt
     1861 gggagcaagc ataattggtg gatgttgtgg aacagatgaa aagtatattg ctgccatcaa
     1921 aaaagctatt caagaagtgt ctgctttcaa accacaactt tagactttct cggtgaggga
     1981 aggattgttt cccctcctaa aaaagaggct atttcttcca gcttataaaa aggaggggat
     2041 tttactcctt cccgggcagg atttcgatcc agagcattaa tttgcacccg atgaggagaa
     2101 acgagggcga ttttctcttt gagaaaattt agctctcttt ctgagtcgtt tatcccttct
     2161 actataaaaa tctctaacca tatttccccc gggaaatgtt tacgtagttt aattaagctc
     2221 tctatgtaag aatttagagt aatccgagga tgaggctggt caatggcctg gagagcttga
     2281 ggagaagcag cgtccaggga aggaattatg atatccattt cctttacttc ttctgaaagt
     2341 tcttcataga aagcaaaaag agttccgttg gtgattagga ctaatttttg ctcggggaag
     2401 agttttttga taaaatgggc gatttctcct aaagcagaat gaagcgtggg ctctccccag
     2461 ccggcaaaag tgatacaata aggaggcgcg ctgcgaaatt gggaagaggc agcatatttt
     2521 ttgagacttt ctatcacttc ttgagtggga atatattctt ttctttctgt ggttaaattg
     2581 gtggtcactc cgcattcaca atatatgcag ttcaagctgc aagttttata agggaggaga
     2641 ttgattccca gagaactgcc taatcttcgt gataatatgg gtccaaaaat acattttttt
     2701 gccataagtt ttccttctct ctacaaatct tctaagaaaa tctttaaccc cactaatata
     2761 atagctattc ctcccattac ctcgggagag gaaaaaactc ctcttatttt atgtcctaag
     2821 gaaactcctg aataagtcat aaatagtgct actattccga taatcagagc ggaaaaccag
     2881 atatttttac ccataagggc aaaagagaat cctaccgcta aggcatcgat actggtagct
     2941 aaagaagcaa gaaagagagg ccagcctcga ctgaagtcta taagaggtgt gtcgctatct
     3001 cttttccacc cttcataaat catcttccct cccactaaag atagtattcc taaaactacc
     3061 cagtggtcaa aagagctgat aaatcgtgct acttgtcgac ccactccaaa acccacgagt
     3121 ggcatgaaaa actgaaaagc accaaaggaa aacgagagac gcaatctggc atctcgcggg
     3181 caggccttct ggcacatccc gaaggctgta gctaaagaga aggcgtctaa acctaatcct
     3241 atagcgataa taaatgtttc taacaatgag atccttcctt tttaaaacct ggtggattat
     3301 accataaagg cttttgcttt ttaaagagat atcgaaactt aatatttatt ctcattcaca
     3361 gattaatttt aaaaagtttt tgtccgaaaa accgattcaa ttgaactact ttttgtaatg
     3421 aattataata catttaaata attacaggga ggaaaaaatg atggctaacc tttcggttga
     3481 tgaagaaata atttctccag accaagaaga agccctggag ctgattttac agaaattcaa
     3541 agggaaacca ggtagtttaa ttcaggcttt gcatgctgcc cagaacttga ttggttatct
     3601 accacccccg gttttgaaaa aggtttccca tgttctggac attcccttaa gcgaaattta
     3661 cgggatagtt actttttatc actttttctc tctcaagcct cgaggaaaac atattattca
     3721 ggtttgcttg ggaacagctt gctatgtgag aggaggacaa gaaattctca atcgtttgga
     3781 ggaggattta gaaattggag taggagaggt gaccgaagac agattgtatt ccctggaagt
     3841 aatgcgttgt gctggagctt gtggtttagc accgatagtt aagattgaca acgatattta
     3901 taagagggta aaccctgctc aaattatgga tatagttaag agctattcct aattttatga
     3961 aggaactttc tttacacatt ttagatgtag tggaaaattc tctccgggct aaagcaaaaa
     4021 aagtaaggat ttctatcctg gaggataccc gggaaaatcg tctggagttg gttattgagg
     4081 atgacggaga aggcatggat gaggaaacat taaaaatggt gagggaccct tttttttctg
     4141 gacagggtaa gaaggtggga atgggcattc ctctcttaga tcaattaacc aggcaatgcg
     4201 aggggaattt agagatatta tcggaaaaag gtaaaggaac cactgtgcga gcaacttttc
     4261 aaagagataa tcttgattta ccccctctgg gaaaactggc tgatactctt tttgtgatta
     4321 tggcttctca tccagaagtg gagctggaat ttatccaccg aatagatgat tgccagtggt
     4381 tatttaacag taaaaagata gaggaagaat tagatttaac cgaggaaaac cggggtgtac
     4441 ttttgcctgc tattcgggag tggattgagt atcaagaaga aatattgaag ggaggaattg
     4501 ggaatgaaga taagtagtat agaagattta agacgaatta aagaagaagc gcaaaaaaga
     4561 actttattgc gagaaggaaa ggctgattat agagtggtag tgcatatggg gacctgtggt
     4621 atagcagcag gagcgagaga ggtaatgtca gccattctgg atgaaattgc taaacgaggt
     4681 ctcactaacg ttatggttac ccaggccggt tgtatcggac tctgtgatcg agaacctctg
     4741 gtgagcgtgg aaaaggggga ggagcgaatt ttatatggtg atattacccc ggacagggct
     4801 cgacaaatca tagcttctca cctggttaat ggccagattg taggagattg ggtagtgcat
     4861 gctgaaggca gggcgaggag gtaaagtgga tggctgaaca ggaaagatgt caggtacttc
     4921 tatgtgctgg tgcggcttgt atttcttctg gggctttaaa aataaaggaa gctctggtaa
     4981 gggagcttca gaaaaatgcc ttggataatg aggtaaggtt gatcgagact ggttgtgtgg
     5041 gtccttgtaa tttagggcca ctggccatca tctacccgga gggggttttt tatcagaaac
     5101 taactcctga agatgcaaaa gagatagttg aggaacatct tcttaaaggg agggtggtag
     5161 agaggttact atatcagccg ccccaggaag aaagaaaaaa gtttttgcga gaaattgact
     5221 tttttgataa acaactaaaa attgctctgc gaaattgtgg tttcatcaat cctctgaaca
     5281 ttgaagaata tatcgctcgt gacggatatt tagctctctc tcgagttttg actgagatga
     5341 ctcctgagga agtaatagca gaagttaaaa agtcaggtct tctgggtcga ggcggagcag
     5401 gtttccctac gggcttaaag tgggagctta cccggcaagc taaaggctat cccaagttta
     5461 tcatttgtaa tgctgatgag ggagacccgg gagcttttat ggatagaagt attttagaag
     5521 gtgacccgca ttccgtaatt gaggccatga ctatcgccgg ctatgcagta ggagcagaaa
     5581 aaggctatgt ttacgttcgg gcagaatacc ctttagcggt agagcgactg gaatatgcta
     5641 taaatcaagc tcgagaatat ggtcttctgg gagaagatat actcaacagt ggttttaact
     5701 ttgatgtcga gattcgtatt ggagcgggag cttttgtctg tggagaagaa acagctttga
     5761 ttgcatctgt agaaggaaaa aggggcatgc ctcgccctaa acctccttat cctgctcagg
     5821 aaggattatg ggggaaaccc acagtgatta acaatgtgga aacctatgcc aatattcccc
     5881 ccatcatatt acagggggca gattggttcc ggtctattgg agcagagaat agcaaaggta
     5941 ctaaagtttt tgctctggct ggagatataa ataataccgg tttagtagaa gtgcctatgg
     6001 gtattagcct gggagaggtc atttatgatt taggaggagg tattcgggga ggcaaaaaac
     6061 taaaagcggt gcagattgga gggccttcag gaggatgtat cccggttggg tatctcaatg
     6121 ttaaaatcga ttacgattct ctgaaagagt taggagccat tatgggctcg ggtggtctca
     6181 tagttatgga tgaagatacc tgcatggtgg atctggctcg atttttcctg gagtttgttc
     6241 aagacgaatc gtgcgggaaa tgcactccct gccgaatagg cactaaaagg atgttagaaa
     6301 ttttacagag gattacccag ggagaaggaa gagaaggaga tatagagcgt ttggaagatt
     6361 tagctaacca gatcaagacc actgctttgt gcggtctggg gcaaactgct cccaatccgg
     6421 ttttgagtac tattcattat tttcgggacg aatacgaggc tcacattcga gagaaaaaat
     6481 gtccagcagt ggtgtgccgg ccattatttg tgtctccctg tcagcatgcc tgtcctctgg
     6541 gtataaatat tcccggttat atttcttgca ttaaagaggg cgattatttg ggcgctttac
     6601 agattatcag ggaagataac ccgcttcctt cagtttgtgg gcgggtttgt catcggcttt
     6661 gcgaaagtaa gtgcaggcgt ggagatatgg atgagccggt agctattgat gatttaaagc
     6721 gttttgtggc tgattatgcc cggcaaaatc ggatagcctt gccagtggtt atggatagaa
     6781 aacgagatga atcagtagct gtggtgggtt ctggtcctgc gggactcact tgtgcttatt
     6841 atctggcccg gctgggttat tcggtaacca tttttgaagc tcttcctgta gcgggaggaa
     6901 tgttagctgc aggtattcct ccttatcgtt tgcctcgaga ggctctggaa tatgacatta
     6961 atcaaattaa ggctatggga gtaaagatag agctcaataa agcggtgggt aaagaaatta
     7021 gctggaagga agtacgggaa aagtttgatg ctgttttcct gggtatagga gcctggaaga
     7081 gcgctccttt aggtattcct ggtgaaaatc tctctggagt aattgccgct ctcgactttc
     7141 tacgcgactt taatttagga ggctatgttg aggttccccg tcgagcagtg gtggtgggag
     7201 gaggaaatgc agccatagac gctgctcgta ctcttcttcg tctgggagca gaggaggtaa
     7261 acgtagttta tcggcgtacc agaggtgaaa tgcctgctat ttccgaagaa gtagaagctg
     7321 cagaagaaga gggcgttatt ttccactttt tgatggctcc acttgaagtg ataggagaaa
     7381 atggcaaagt aaaaggtatc aggttgcaaa ggatgaagct gggagaattt gaccggagtg
     7441 gaagaaggag acctatccct gtagaaggtg cagaagcttt ctgggaatgt gatatggtgg
     7501 tggaagcagt gggtcaaatt ccggaagtgg agagtttagg agagatgtta tctgtggacc
     7561 atcgaggtaa tattttagtg gaccgttatt ctctttctac ctcccttcct ggagtttttg
     7621 ctggagggga tgctataggt gaagaggcta ccgtggttca tgctatggct ctgggtaaaa
     7681 aagcagcctt tagtattcac cagtatctgc gggatttgaa gcctgaggaa gaagaagtta
     7741 taatccggcc agagcgaccc aaaattctgg aggagccacc agttttacaa gaaatccctc
     7801 gagttaaacc accggagcta cctgtaagag agcgagtgtg ttctttttcg gaagtgaagc
     7861 tctgtctgga agaggaagac gctcgtcggg aggcagaacg atgtttgcgc tgcgatctgg
     7921 aaaaagtatt gaaaaaatac caggaaatag ttatggctga ggaggaggtt caatgactgg
     7981 attagcagtt tatgaggaga tagatgaaat tatcgggaaa catcaagagg agaaaactcc
     8041 tcttctttct attctgcaag agatacagga gagatggcga tatctccccg gggatgttct
     8101 aggatatgta tcccggaaat tagatgttcc gtccagtatg gtatggggag tagccacttt
     8161 ttattccttt ctgaaaacag aaccggcagg tgaatacgtc atcagggttt gttgcaatgc
     8221 tccctgtcat gttaatggtt ctgtggacgt tttgaaaacc ctgcaaaagg agttaggtat
     8281 taaagaaggg gaaacttccc gagatggcaa atttactctg gagacgacca gctgtttggg
     8341 agtttgtggt gttgctcccg ctatgatgat taacgatgta acttatggca atctctcaga
     8401 agatagaatc cgagagattt tagcccttta ccggtaggag gacgcgtatg ttttatcgca
     8461 gtcatgtttt agtggagatg accagtaatt ctctggttcg gggagcagct caaattaaag
     8521 agagactgga gagagaaatt gaaaggttag gtttagataa agaaattaaa gttctggaaa
     8581 ctggtagcct tggggcagcc cttccctctc cttttgtggt ggtttaccct gataacatcg
     8641 tttatgctcc ggttacagtg agcgatgtaa aaaggattgt agaagagcat ttctggaaag
     8701 gaagaccagt gggagatttg attttctctc cttcagaacg gagagaaatt ggtgtttttt
     8761 taccttattt tccactgact aaagaaaaaa gggtggtttt acgcaatagc ggtcagattg
     8821 atcccactaa cattgaagaa tatatcgctc gtgacggata tttagctctc tctcgagttt
     8881 tgactgagat gactcctgag gaagtaatag cagaagttaa aaagtcaggt cttctgggtc
     8941 gaggcggagc aggtttccct acgggcttaa agtgggagtt tactaaaaat gcagaaggag
     9001 tggaaaaata tatagtatgc aatgctgatg agggagagcc aggaactttt aaagaccgac
     9061 ttattttaga aggtgaccct cattccataa ttgaagccat ggctattgcc ggttatgcag
     9121 taggagctgg agagggttac atctatgtgc gaggagaata tccccagagt attaaccatt
     9181 tgagaatagc cattgagcaa gcccgagaat atggtttgtt aggggaaaat atattgggtt
     9241 cctcctttag ttttgaagtg cgtattcgta aaggggcagg agcctacata tgtggagaag
     9301 agactgctct tttagagtcc attgagggga aaagaggaat acctcgaaac aagcctcctt
     9361 atcctgctca agagggatta tgggggaaac caacagtaat taacaatgta gaaactctgg
     9421 ccaatattcc tcctatcata ttacagggcg cagattggtt tcggtctatt ggaactccca
     9481 cctgtacagg tactaaagtt tttaccttga ctggtaatgt aaacaatctg ggcttaatcg
     9541 aagttcctat gggtattacc ttaagggaac tgatttttga ggtgggagga ggtatccggg
     9601 ggggtcataa cctgaaatta gttcaaactg gtggtccctc aggtggtacg atgactcctg
     9661 accttttaga tgttccgatg gcttttgata ctctttcccg ttacgattcg gctttagggt
     9721 cgggagcact tacggtaata gatgatactc actgtatcgt agatgtggtt agaaatttta
     9781 tggagttttt tctttatgaa tcgtgcggga aatgtactcc ctgccgggtg gggactaaga
     9841 ggatagtgga aattctaaat agaataagtg aaggcagagg agaaccctcc gatcttgaaa
     9901 atctacgcta tatgggagaa catataaaaa gtacatcttt ttgtggttta ggacaggctg
     9961 ctcctaatcc tcttttgcgc tctttaagtt tttttgagga ggaatttaga gctcacctgg
    10021 aagagaaaag gtgtcctgtg gatacttgtt ctatgaaaac taaaactaaa attttgattt
    10081 gaataaggag ttgaagagcc catggctaaa atagtagagg aaatgaaaga aggaacgggt
    10141 caaaaaaagt tggttactat ttatttagat gggagagaag tgaaggtacc agaaggggtt
    10201 agtatcttag aagcttgcaa tatggaaggt ataaaaattc ccaccctttg ttattttgag
    10261 ggattaactc cctggggagg atgtcgaatc tgcatggtgg aagtagaggg acaacctaat
    10321 cttgtggcct cctgtgttac tgacgtgcga gaaggtatga aaatcaaaac ttcctctaaa
    10381 aaagtgagag aagctcgtaa aactaatctc gagcttttgc tttctaatca tcctctggat
    10441 tgtcaattgt gtgaccgcaa tggttcttgc gagttacagg atttatccta tcagtttggt
    10501 gtgcgagaaa tccgctttcc tggagagaaa aagacttacg aggtggatag aagcagccct
    10561 tcaattaaga gagatgctaa ccgctgcatc ctgtgtggtc gctgtatcag agcttgccag
    10621 gaaatacagg cagtttttgc catcgatttt gctaatcgag gatttgatag tttagtcagt
    10681 ccttcgctgg ggcttccctt aggagagagc gtatgtgtta actgtggaca gtgtattctg
    10741 gcctgtccca caggagcact ttctgaagtt tctcatgtgg aaatggtatg ggaagcctta
    10801 gaagatccag aaaaatttgt aatcttgcaa actgctccag ctataagagt aagtattggg
    10861 gaaccttttg gtttaccccc gggaacagtg actactggta aaatggtagc agcattccgg
    10921 agattgggat ttgaccgggt gtttgatact aattttgctg ccgacctcac catta
//
LOCUS       DAOPSS010000051        10441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_5371_length_10441_cov_8.489601, whole genome shotgun sequence.
ACCESSION   DAOPSS010000051 DAOPSS010000000
VERSION     DAOPSS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5371_length_10441_cov_8.489601"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..458
                     /locus_tag="PKN31_04455"
     CDS             219..458
                     /locus_tag="PKN31_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOA99211.1"
                     /translation="MVVQNLEQIKEWVASIVGEEVVIEVNKGRKKTITKKGVIEKAYP
                     FFFTMITEVDGSSQRASFNYADILTKTIELEITHS"
     gene            511..1215
                     /locus_tag="PKN31_04460"
     CDS             511..1215
                     /locus_tag="PKN31_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOA99212.1"
                     /translation="MEKLLDFPYAVDVKEAKEIQEKLKSLLSFDFPYEDKDIDLVAGI
                     DVSYNTEKEASAAVVVLDFPSLRVREVSWGKAPPPFPYIPGFLSFREGPAIEKALSLL
                     CSQPQIFFFDGQGIAHPRGVGLATHLGILFGMVSVGVAKSNLVGNFEEPPEKQGSFSW
                     IEYQGEKVGMALRTKEKVKPVFVSPGNRIDLEKAKHWTLLVTTKYRLPEPVRQAHIFS
                     EKIKKGGEEYGRYSFS"
     gene            1193..1510
                     /locus_tag="PKN31_04465"
     CDS             1193..1510
                     /locus_tag="PKN31_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOA99213.1"
                     /translation="MDDIPFHELEEESTTENKVVITVVGKDRIGIIAAITSCLAEREV
                     NIEDITQKILGEYFTMILVGDISQCPVSLAELHRDLNHKGEEIGVKVYMQHASIFRAM
                     HRI"
     gene            1519..2883
                     /locus_tag="PKN31_04470"
     CDS             1519..2883
                     /locus_tag="PKN31_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOA99214.1"
                     /translation="MLFDRSEILETLRMLEIENLDVRTVTLGINLLDCRSGDFSSTIE
                     KVAEKIRHLSLPFHEIVEETFIKYGIPVVNRRIAISPVSFLLDGRERKEEVVALARRL
                     DDLARELQVDFIGGFSALVPKGCTPGDLLLIDSLPLVLSQTQRICGSVNLASSRSGIN
                     VDAILKMGEVVKETSLLTAQEGGIGCAKLVIFANAPEDNPFMAGAFHGPGEADAALNV
                     GVSGPGVIKEAIRRKPEADLGEIAEVVKRTAFKITRVGELIGKEIAQKLGVPFGIVDL
                     SLAPTPRVGDSVAEILEEMGLEKCGAWGSTLALALLTDACKKGGAMASSSVGGLSGAF
                     IPVSEDAGMTESVKVGALKLEKLEAMTSVCSVGLDMIAIPGEVSKETISAIIGDEMAI
                     GVVNHKTVGVRIIPVLGKKPGDRVVWGGLLGETVVMEVNPFQSKNFISRGGRVPAPIT
                     SLKN"
     gene            complement(2880..3374)
                     /locus_tag="PKN31_04475"
     CDS             complement(2880..3374)
                     /locus_tag="PKN31_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOA99215.1"
                     /translation="MIISGKALKYGDNIDTDVIIAARYLTSTDPAELARHCLESLDPT
                     FSEKASSAILVAGKNFGCGSSREHAPLAIKASGAKAVIAASFARIFYRNAVNIGLPIL
                     ESEEAAEKIKEGDQLEIDLDKGEIKDLTSGDTFQVNPFPPFLQEIIERGGLMNLVKER
                     VKSD"
     gene            complement(3389..4648)
                     /gene="leuC"
                     /locus_tag="PKN31_04480"
     CDS             complement(3389..4648)
                     /gene="leuC"
                     /locus_tag="PKN31_04480"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOA99216.1"
                     /translation="MGMTITEKIIAAHAQKEKVIPGELVQVKIDLALANDITAPLGIK
                     ELEKQKIDRIFDPDKVVLVADHNTPCKDIASAQNVKLMRDFARKYGISHFFEGGKAGI
                     EHVLLPEEGLIAPGDLVIGADSHTCTYGALGAFSTGMGSTDIAASWVLGETWLRVPES
                     ILFLYEGKRKPFVTGKDLILFTIGKIGVDGARYCAMEFRGSVISELSLDERFTMANMA
                     VEAGAKNGIMEADSKVLNYVSGRTSHLSRVYQSDPDARWKEIIEIDVSSLEPQVAFPH
                     LPSNTHSVYSIPKIEIDQVVIGSCTNGRMEDLRMAAQVLKGREVNPRVRLLIFPGTPS
                     VLKQAEKEGLLRIFLEAGGIIAPPSCGPCLGGHLGVLAEGEKCVSTTNRNFLGRMGHV
                     KSEVYLANPYVAASSAVAGYIAAPDQI"
     gene            complement(4650..6194)
                     /locus_tag="PKN31_04485"
     CDS             complement(4650..6194)
                     /locus_tag="PKN31_04485"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOA99217.1"
                     /translation="MRKIRIFDTTLRDGEQSPGVSLNPQEKLEIAKQLAQLNVDVIEA
                     GFPIASPGDAEGVKLIAQQIKGPAICALARCKEKDIEVAGEALEHAERPLIHVFLATS
                     DIHLQHKLHLTREEALEEAVRAVKKARNYTDEVEFSAEDATRSDWEYLSQIYAAVIKA
                     GATVLNIPDTVGYTIPEEMKELIIYLLEHTPGMDKVEISVHCHNDLGLATANSLAAIS
                     AGATQIECTINGIGERAGNAALEEIAMALHVRKDRFQARTSLNLQEIYRTSRLVSRLT
                     GMLVQPNKAIVGQNAFAHEAGIHQDGVLKDKLTYEIMDAQLIGREERVLVLGKHSGRH
                     AFAKKLEELGYQLSEEELNQAFQRFKQLADQKKEITEADLKAIVSDEVSRSEDIYRLD
                     FVHVCSGNNILPTATVRLIREGGEILEGVSTGVGPVDAAYKAISKMLDISPNLVNYTL
                     QSITGGTEALGEVIVRIIGEDQEYVGRGSNQDVVVASVLAYLAAINRMAQKKKKINPQ
                     KDYKEN"
     gene            complement(6207..7199)
                     /gene="ilvC"
                     /locus_tag="PKN31_04490"
     CDS             complement(6207..7199)
                     /gene="ilvC"
                     /locus_tag="PKN31_04490"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOA99218.1"
                     /translation="MSKVYYDEDANLEVLQGKTIAIIGFGSQGSAQAQNLRDSGLEVI
                     VSDLPGTPGFEKARNAGFSPLSAAEAAQKGDVIQILAPDQYQAGIYKESIAPHLKAGG
                     VLMFSHGFNIHYHQIIPPRDVDVIMVAPKGPGDLVRRMYQEGKGVPSLIAVYQDASGK
                     AKDIALAYARGIGATRAGVLETTFQEETETDLFGEQAVLCGGLTALIKAGFETLVEAG
                     YQPEVAYFECLHEMKLIVDLVYEGGLTNMRQVVSDTAEYGDLTRGPRVINDKVREEMK
                     KILAEIQDGSFAREWILENQANRPVYYALRNKDAQHLIEKTGNTLRGMMPWLKK"
     gene            complement(7228..7722)
                     /gene="ilvN"
                     /locus_tag="PKN31_04495"
     CDS             complement(7228..7722)
                     /gene="ilvN"
                     /locus_tag="PKN31_04495"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOA99219.1"
                     /translation="MEHIISVLVENKPRVLARVSSLFARRGYNIESLAVGPTNDPTIS
                     RITLVVEGNTDTLEQITKQLYKLIDVIKVGDFTQASYVDRELALIKVNAPSDIRAEIV
                     QTVEIFRARVVDVEEKSLIVEVTGTPDKIDALEQLLRKYGIIEMTRTGRIALARGSQS
                     LQEK"
     gene            complement(7743..9407)
                     /gene="ilvB"
                     /locus_tag="PKN31_04500"
     CDS             complement(7743..9407)
                     /gene="ilvB"
                     /locus_tag="PKN31_04500"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOA99220.1"
                     /translation="MKMRGAQMLIELLKREGVEVIFGYPGGAVLDIYDALMDDKDITH
                     ILVRHEQGAAHAADGYARTTGKVGVCIATSGPGATNLVTGLANAYMDSVPLVAITGQV
                     ATSLIGKDAFQEADTTGITMPITKHNFLVTSFEELPDVVRQAFYIASTGRPGPVVIDL
                     PKDIQQQEGEFIFPEKVKITGYRPTYRGHAHQIKVAAQAIQEAERPVLIAGGGVISSG
                     ASEALLKLAEKADIPVTHTLMGKGAFPESHPLSLGMLGMHGTYWANKAVMNCDLLIAV
                     GMRFDDRVTGSLATFAPRARIIHIDIDPAEIGKNVKPSIPIVGDAKMVLSELEKKVEE
                     KKRSSWRGKTEEWKKEYAPRVQEGLSSRRVIEKIYELTHGDNPIVVTDVGQHQMWAAQ
                     IYRCEVPRQWASSGGLGTMGYGLPASIGAQVGNPDRSVVLISGDGSIFMKIQELVTAV
                     NYCLPIKIFVINNSYLGMVRQWQGCLYGGRYSCTKLQNPDLVSLVRAFGGEGLKVEKE
                     EELEEVISRALEVKNKPVLVDCRVAEEENVLPFVPPGKSLEEMILE"
     gene            complement(9423..>10441)
                     /locus_tag="PKN31_04505"
     CDS             complement(9423..>10441)
                     /locus_tag="PKN31_04505"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOA99221.1"
                     /translation="ATALAMSAQKRRIAYESGKRIVELVKENTVAREILNEKSLRNAI
                     KMDLSLGGSTNTVLHLLALAQELGISAISLTTFDELSRKIPHLVMLRPGGELFLEDLH
                     FAGGIPAVEKTLGELLEDNLTVSGKTVREIQKAVEEADVDREIIRTLDNPHSPEGGIA
                     VLYGSLAPDGAVVKQSAVEPEMMVFRGKAICFDSEEEAMEAITHQKVSPGSVIVIRFE
                     GPKGGPGMREMLAPTSAVVGMGLSKEVALITDGRFSGGTRGPCIGHIAPEAFEGGPIA
                     LIREGDEILIDIPHRQIELLVNKEELERRKKDFTPPPPKYKTGLLGRYIKLARPTSQG
                     ASME"
BASE COUNT         2688 a         2445 c         2285 g         3023 t
ORIGIN      
        1 ttctctttat aggctcgttt taaatcctct aggagatgac tcaaagtggt aaagtggaaa
       61 aagactacat tggcgacaat ccttttccag gatggcataa aaataagtaa aaacaaagat
      121 accccggcaa tggattgtgg agaacaatag tttgaaaaat ttgcttttca cccttttttg
      181 gtgtataata gaggaaaatt tctagggaat gatgttcttt ggtagttcag aatcttgagc
      241 aaatcaaaga atgggtggca tctattgtgg gagaagaagt agttatcgaa gtcaacaaag
      301 gaagaaaaaa aacgataacc aaaaaaggag tgatagaaaa agcctatccc ttctttttca
      361 ctatgataac ggaggtagat ggttctagcc agagagcttc ctttaattac gctgacattc
      421 ttactaaaac catagaatta gaaataactc actcttaaaa atttagtcca tcgtcttcct
      481 cttttttcct ctttggttta aaatgcttct atggagaaat tgcttgattt tccctatgct
      541 gtagacgtaa aagaagcaaa ggaaatacag gagaaattga aaagcttatt atcttttgat
      601 tttccttacg aggataaaga tattgattta gtagcaggaa tagatgtctc ctacaatacc
      661 gaaaaagaag cttcagcggc agtggtggtt ttagattttc cttctttgag agtgagggaa
      721 gtatcctggg gtaaagctcc tccccctttt ccttatatcc cgggattttt atcttttcga
      781 gagggtcccg ctattgaaaa agctctatct cttttgtgtt ctcagcccca gatttttttc
      841 tttgatggac agggtatcgc tcacccccgg ggagtggggt tagccactca tctgggaatt
      901 ttattcggga tggtttctgt gggggtagcc aaaagcaatt tggtaggcaa tttcgaagag
      961 cctcccgaaa aacagggctc tttttcctgg atagaatacc agggagaaaa agtggggatg
     1021 gcgctacgca ccaaagaaaa agtgaaaccc gtttttgttt cgccgggcaa tagaattgac
     1081 ttagagaagg caaaacactg gacgctatta gtaactacta aatatcgttt acccgagcct
     1141 gttcgccagg ctcatatttt tagtgaaaaa atcaaaaaag gcggagaaga atatggacga
     1201 tattcctttt catgagctgg aagaagagag cactacagaa aacaaagtgg tcatcaccgt
     1261 ggtgggtaaa gatagaatag gaattattgc tgctattact tcttgccttg ccgagcgcga
     1321 ggtaaacatc gaagatatca cccaaaaaat attgggagag tattttacca tgattttggt
     1381 gggagacata tcccaatgcc cggtgagcct tgcagaacta caccgcgacc tcaaccacaa
     1441 aggggaggag atcggggtta aagtctatat gcagcacgcc agcattttcc gggctatgca
     1501 tcggatttga ggtgaaaaat gctttttgac cgctcagaaa ttttagaaac gctgcgtatg
     1561 ctggaaatag agaatttaga tgttcgaacg gtaactctgg ggattaatct tctggattgc
     1621 cgctcgggtg atttttcttc caccatagaa aaagtagcag aaaagattcg tcatctctct
     1681 cttcctttcc atgagatagt agaagaaact tttatcaagt atgggattcc tgtagtgaac
     1741 cgacggatag ctatatcccc ggtgtctttt cttttagacg gtagagaaag aaaagaagaa
     1801 gttgtagctc tggcccggag attggacgat ttagcacgag agctccaggt tgattttata
     1861 gggggattca gtgctttagt tcccaaagga tgtacccccg gagacttgtt gcttattgat
     1921 tctctccccc ttgttctctc acaaacccag agaatttgtg gctcggtaaa tttagcttcc
     1981 agccgcagcg gaataaatgt ggatgctatt ttaaagatgg gagaggtagt caaagaaacc
     2041 tctcttttaa ccgctcaaga aggaggcata gggtgcgcca agctggtgat ttttgccaat
     2101 gccccagaag acaatccttt tatggctgga gctttccacg gaccgggaga agcagatgcg
     2161 gccttgaatg tgggagtaag tggcccggga gttatcaaag aagccatccg cagaaaacca
     2221 gaagcagatt taggagaaat agctgaagtg gtaaaaagga ctgctttcaa aatcacaaga
     2281 gtgggagagt tgataggtaa agaaatagct caaaagctgg gagtaccttt cggaattgta
     2341 gatttatctc tggctcctac accccgggtg ggagatagcg tagcggagat tttagaggag
     2401 atgggtttag aaaaatgtgg agcctggggt tccactttag ctctggctct tttaactgat
     2461 gcctgtaaaa aaggaggagc tatggcttct tcttcggtgg gaggattaag cggagctttt
     2521 atcccggtta gtgaagatgc cgggatgacc gaatcggtga aagtgggagc cttgaaattg
     2581 gaaaaattag aagctatgac cagtgtttgt tcggtaggtt tagatatgat tgccatccct
     2641 ggcgaggtat ccaaagagac aatttcggcc atcatagggg atgagatggc tattggagtg
     2701 gtgaaccaca aaaccgtggg ggtgcggata ataccagttc tgggaaagaa gcccggagat
     2761 agagtagtgt gggggggact cttaggagag acagtagtta tggaagtcaa tcccttccag
     2821 agtaaaaatt ttatctcccg gggaggaaga gtccccgctc ctattaccag tctaaaaaat
     2881 tagtcagatt taactctttc ttttactaaa ttcatcaatc ctccccgctc tattatctct
     2941 tgcaagaaag gaggaaaggg gttaacttgg aaagtatccc cactggttaa gtctttgatt
     3001 tctcctttat ccaggtcgat ttccagttgg tctccctctt taattttttc cgctgcttct
     3061 tctgattcca gtatgggtag accaatgttt acagcattgc gataaaaaat acgggcaaaa
     3121 gaagcggcaa taactgcctt cgctcctgat gctttaatag ccaaaggagc atgctcacgg
     3181 gaagagccac aaccgaaatt tttccctgct accaggatag cagaagaagc tttctcggag
     3241 aaggtggggt ctaaactttc taagcaatgc cgagctaact ctgcagggtc agtggatgtg
     3301 agatagcgag ccgcgattat tacatcagta tctatattat ccccgtattt cagagctttt
     3361 ccacttatta tcattttgtc acctcgtctt aaatttgatc gggagcagca atgtagcctg
     3421 ccacagcgga gctcgctgct acataggggt tggcaagata aacctcgctc tttacatgtc
     3481 ccattcttcc taagaagttt cggttagtgg tagatacaca tttttctcct tctgctaaaa
     3541 ctcccagatg tcccccgaga cacggtccac aagaaggagg agcaattatc cctcctgctt
     3601 ctaagaaaat ccgcaacaac ccctcttttt cggcttgttt taaaaccgaa ggagttcccg
     3661 ggaaaatcaa gagccttacc cgggggttga cttcccggcc ttttagcact tgcgctgcca
     3721 tcctcaaatc ttccatacgg ccattggtgc aggagccaat caccacctgg tcaatctcta
     3781 ttttggggat agaataaacg gaatgagtat tgctgggtag gtggggaaaa gccacctgag
     3841 gctcaagaga ggaaacatca atttctatta tttccttcca ccgagcatca gggtcagatt
     3901 ggtaaactcg agagagatga gaggttctcc cggaaacata gttgaggacc ttgctatcgg
     3961 cttccattat cccattcttt gctccggctt ctactgccat attggccata gtgaaacgct
     4021 catccagaga caattccgat attactgaac ccctgaactc catagcacaa tatcttgctc
     4081 catctacccc aatttttcct atggtgaaaa ggatgaggtc ttttccggtg acaaacggtt
     4141 tccttttccc ctcatagagg aaaagaattg attctggaac tcgcagccag gtttctccca
     4201 acacccagct ggcggcgata tcagtgcttc ccatccccgt ggaaaacgct cccagcgctc
     4261 cataggtaca ggtatgagaa tcagctccaa tcaccagatc cccgggagct atcagccctt
     4321 cttcgggaag aagcacatgt tctattcccg cttttcctcc ctcaaaaaaa tgagatatcc
     4381 cgtatttccg ggcaaagtct cgcatcaact ttacgttttg agcagaagcg atgtctttac
     4441 aaggagtgtt atggtcggca accaaaacta ccttatcagg gtcaaagatt cggtcaattt
     4501 tctgtttttc cagttccttg atgcccagag gagcagtgat atcattggct aaagccaggt
     4561 caatttttac ttgcaccagt tctccgggaa ttactttttc tttttgagcg tgagcagcta
     4621 tgattttttc agttatggtc atacccattt tagttctcct tatagtcttt ttgagggttg
     4681 attttcttct tcttttgagc cattcggttt atggcagcaa gataggctaa aacactggct
     4741 accaccacat cctggttaga tcctcttcct acatattcct ggtcttcccc tattattctc
     4801 acaataactt ctcccagcgc ttccgttcct ccggtgatag attgcaaagt gtagttaact
     4861 aaattgggag atatatccag cattttgctt atggctttgt aagctgcatc tactggaccc
     4921 actccggtgg ataccccctc cagtatttct ccaccttctc tgattagcct cacagtagca
     4981 gtgggtaaaa tgttatttcc tgagcaaacg tgcacaaaat ccagacgata tatgtcttct
     5041 gagcgggata cctcatcaga aacaatagcc ttgagatctg cttcggtgat ttcttttttc
     5101 tggtcggcta attgtttaaa gcgctggaaa gcctgattga gctcttcttc ggagagttga
     5161 tatcctaact cttctaattt tttagcaaaa gcgtgacgtc cggagtgttt acccaatacc
     5221 agcacccttt cttctctacc gataagctga gcatccatta tctcgtaagt tagtttatct
     5281 ttaagtactc catcctgatg aattccggcc tcatgagcga aggcattctg tcccactatg
     5341 gctttattgg gttgcaccag catcccggtg agtctgctca ccaggcggct ggtgcgatat
     5401 atttcctgaa gattgagaga agtccgggct tgaaaacggt cttttctaac atgcagcgcc
     5461 atagctattt cctctaaggc ggcattaccg gctctttctc cgataccatt tatggtgcac
     5521 tctatctgag tagcaccagc ggaaatcgct gctaaagaat tagcagtggc taaccccaag
     5581 tcgttgtggc agtggacgct tatctcaact ttgtccatac cgggggtatg ttccagaaga
     5641 taaataatca gttctttcat ttcctcagga atagtgtatc ccaccgtatc gggaatgttg
     5701 agaactgtgg ctcccgcttt aatcactgca gcgtaaattt gggagagata ctcccaatcg
     5761 gagcgagtgg catcctcagc agaaaattcc acctcatcag tatagttccg ggctttttta
     5821 actgcccgga ctgcttcctc aagagcttct tctcttgtta agtgcagttt gtgctggaga
     5881 tgaatatcag aagtagccaa aaacacgtga ataagcggcc gctcggcatg ttccaatgct
     5941 tctcctgcca cttcgatatc tttctctttg catcgcgcca gagcacatat agctggtccc
     6001 tttatctgct gagctattaa cttcactcct tcggcatccc caggagaggc aatgggaaag
     6061 cctgcctcta taacgtccac attcagctga gccagttgtt tggctatctc cagtttttct
     6121 tgaggattaa ggcttacccc cggggattgc tccccgtctc gcagggtggt gtcaaaaatc
     6181 cgaattttcc tcattatcct accccctcat tttttgagcc agggcatcat gcctcgcaaa
     6241 gtattccccg ttttctcgat gaggtgttga gcatctttat ttcgcagagc ataatacact
     6301 ggtcgattgg cttggttttc cagaatccat tcccgggcaa aacttccatc ttgaatctcg
     6361 gctaagattt tcttcatttc ttctctaact ttatcattga ttactcgagg accccgggtt
     6421 aggtcgccgt attcggcggt atcacttacc acctggcgca tattggtgag ccctccctca
     6481 tataccagat ccacaattaa tttcatctca tgtagacatt caaagtaagc tacttcgggc
     6541 tgatatccgg cttctactaa agtttcaaaa ccagctttaa tcagagcagt caatcctcca
     6601 cagagcactg cttgctcacc aaataggtcg gtttcggtct cttcctgaaa agtggtttct
     6661 aaaactcctg ctcgggttgc cccaattccc cgggcataag ctaaagctat atctttggct
     6721 ttaccgctcg catcttgata gacagcgatg agagaaggta ctccttttcc ttcctggtac
     6781 attctcctta ctaaatctcc cggtcccttg ggagccacca taattacatc cacatcccga
     6841 ggaggaataa tctggtggta gtggatatta aagccgtgag agaacatcag cactcctccg
     6901 gctttaagat gtggagctat agattcttta taaataccgg cttgatattg gtcaggagcc
     6961 agtatctgaa tcacgtctcc tttctgggct gcttctgcag cactcaaagg ggagaacccg
     7021 gcattgcgag ctttttcaaa tccaggagtt ccaggtaaat ctgacactat aacctccaat
     7081 ccactatctc tcaggttctg ggcttgagcg cttccctggc ttccaaaacc gattatggca
     7141 attgtcttac cttgcaaaac ctcgagattg gcatcttcat cgtagtatac tttgctcata
     7201 ataaaacctc cctttttatt ttataagtta tttttcttgt aaagattggc ttcctcgagc
     7261 cagggcgatt ctccccgttc gagtcatttc tatgatgccg tatttacgta ataactgctc
     7321 taaagcatca attttatccg gagtaccggt tacctctaca atcaagcttt tttcctctac
     7381 atccactacc cgggctcgaa atatctccac tgtttgcact atttctgctc tgatatccga
     7441 gggggcgttt actttaatta gagctaactc ccggtctaca taactggcct gggtgaaatc
     7501 tcccactttt atgacatcga tgagtttata aagttgtttg gttatttgtt ccagggtatc
     7561 ggtgtttcct tctaccacta aagtgatacg agaaatggtg gggtcgttag tgggacccac
     7621 ggccagactt tctatgttgt atcctcgccg agcaaaaaga gaagaaaccc gggctaaaac
     7681 acggggtttg ttctctacta aaacagaaat gatatgctcc actgggcacc tccttctctt
     7741 ttttattcca gaatcatctc ttccagtgac tttccaggag gaacaaatgg taggacattt
     7801 tcttcttctg ctactctaca atccaccaat accggcttgt tcttaacctc caaagctctg
     7861 gagatgactt cctccagttc ttcttctttt tctactttca agccctctcc tccgaaagcc
     7921 cggactaaac tgactaaatc aggattttga agcttggtac aggaatagcg ccctccatag
     7981 agacaaccct gccattgccg gaccatacct aaataagaat tgttgatgac aaaaatcttg
     8041 atgggtaagc aataattgac cgcggtaacc agctcctgga ttttcataaa aatgctcccg
     8101 tctccactga ttaaaaccac cgaacggtca gggttaccta cttgagcgcc aatggaagca
     8161 ggtagtccat agcccattgt tccgaggccc cccgagcttg cccattggcg gggcacttca
     8221 cagcggtata tctgtgctgc ccacatctgg tgctgcccca cgtcagtaac tacaatgggg
     8281 ttatcgccat gagtgagctc gtaaattttc tctatcaccc ggcgggaaga aagcccttct
     8341 tgcactcgag gagcatactc ctttttccac tcttctgtct ttcctctcca ggaggaacgt
     8401 tttttctctt ccactttctt ttccagttcc gagagcacca tcttagcatc tcctactatg
     8461 gggatagaag gtttaacatt tttacctatt tctgccgggt caatatcgat atggattatc
     8521 cgagctcgag gggcaaaagt ggccagactc cccgttaccc ggtcgtcaaa gcgcatacct
     8581 acagcaatta aaaggtcaca gttcatcact gctttattag cccagtaagt tccatgcatt
     8641 cccagcatcc ctaaagaaag aggatggctt tcggggaaag ctccttttcc cattaaagta
     8701 tgagtaactg gaatatccgc tttttctgct aacttcagaa gtgcctctga agctccggag
     8761 gatatcaccc ctcctccagc gattaagacg ggtctttctg cttcctggat agcctgagcg
     8821 gctactttaa tttggtgagc gtgtcctcga taagtgggtc ggtagccagt gattttaact
     8881 ttttcaggga aaatgaattc tccttcttgt tgctgaatgt ccttgggtag atcaataacc
     8941 acaggtcctg gtcttccagt ggaggcaatg tagaaagcct gccggacgac atcggggagt
     9001 tcttcaaaac tggttaccaa aaaattatgt ttggtgatgg gcatggttat tcccgtggtg
     9061 tccgcttctt gaaaggcatc tttacctata aggctcgtgg ccacctgtcc ggtgatagcc
     9121 actaaaggca ccgaatccat ataagcattg gcgagcccgg taaccaggtt ggtagccccc
     9181 gggcctgagg tggctataca cactcccact ttacctgtag tgcgggcata gccatcagca
     9241 gcatgagctg ctccctgctc atgtcttacc aggatgtgag ttatatcttt atcatccatc
     9301 aaagcatcat aaatatcaag cactgctcct cctggataac cgaatataac ttctactcct
     9361 tctcttttca aaagctcaat taacatctga gctcctctca ttttcaactt tatcactctc
     9421 ctttattcca tacttgctcc ttgagaagtg gggcgagcaa gcttgatata gcgccccagg
     9481 agcccggttt tatatttagg aggaggggga gtaaaatctt tttttctcct ctctaattct
     9541 tctttgttta ccagaagctc tatctggcgg tgagggatat cgatgaggat ttcatctccc
     9601 tcgcggatta aagctatggg tcctccttca aaagcctcag gggcaatatg tccgatacag
     9661 ggtcctcgag ttcctccgga gaaacgacca tcagtgatta gagccacttc tttacttaag
     9721 cccattccca ctacagcaga agtgggagcc aacatttccc gcattcccgg tccacctttt
     9781 ggtccttcaa agcgaataac tatcacggaa cccggagata ctttctggtg agtaatagct
     9841 tccatggctt cttcttcgct atcgaagcaa atagcttttc ctcgaaacac catcatttct
     9901 ggctctactg ccgattgctt taccacagcc ccatcgggag ccaaagagcc ataaagtaca
     9961 gctattcctc cctctgggga atggggatta tccagggtgc ggattatttc tctatctaca
    10021 tctgcctctt cgactgcttt ttgaatttct ctcacggttt tccccgaaac tgtgaggttg
    10081 tcttctaaga gctctcctaa agttttttct acggcaggaa ttcccccggc aaaatggagg
    10141 tcctctaaga aaagctctcc tcctggccgg agcatcacga gatgaggtat tttccgggat
    10201 agctcatcaa aagtagtaag ggaaatggcg gaaatcccca actcttgagc taaagctaaa
    10261 agatggagta cggtattggt tgagcctcct aaagataagt ccatctttat agcattgcgc
    10321 agagactttt cattgagaat ctcacgggct actgtatttt cttttaccaa ttccacaatt
    10381 cttttcccgc tttcataggc aattcttctt ttttgagccg acatagcaag ggcggtagca
    10441 c
//
LOCUS       DAOPSS010000052        10328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_5452_length_10328_cov_7.092378, whole genome shotgun sequence.
ACCESSION   DAOPSS010000052 DAOPSS010000000
VERSION     DAOPSS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5452_length_10328_cov_7.092378"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /locus_tag="PKN31_04510"
     CDS             <1..1944
                     /locus_tag="PKN31_04510"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOA99222.1"
                     /translation="IYAVFLVRRIYSFDPGNEKMQSLSSAIQEGAMAFLKREYSFLAI
                     FIIGMFILLTLTLGVTVAICYVCGAICSVGAGFVGMRVATRSNCRTTQAATKSISDAL
                     TVAFSSGAVMGMCVVGFGLVGLSLLYLFVGDPDIINGFALGASSVALFARVGGGIYTK
                     AADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGMGADLFESYVGSIIAAMV
                     LGAAPGAIYGGTSGIVFPLLLAGLGVVASIIGTLFVRAKDEKDLAPALNRGTYISAVL
                     TAIFVLISALLVFRDLGTAKAIGPFVASVSGLIAGIIIGLLCQYYTSSDYKPVQQLAE
                     TAKTGPATVIINGLALGMESTVFPLILICAAILLANWAAGIYGIAIAGLGMLSIVGMT
                     VAVDAYGPVADNAGGIAEMADLDPHVRERTDSLDALGNTTAAIGKGFAIASAALTALA
                     LFVAYSQTVGLRIIDLLHPYTVVGLFIGGMMPFLFSSIAMKAVGTAAFKMVEEVRRQF
                     REIKGLMEGKAQPDYARCVDISTRGAIAQMIFPGLLAVIVPIVVGLVLGKEALGGLLA
                     GAIVTGLLMAIMMANAGGAWDNAKKYIEAGNLGGKGTPVHAAAVVGDTVGDPFKDTAG
                     PSLNILIKLMSIVALVFAPLFMR"
     assembly_gap    1999..2098
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2293..4047
                     /gene="dnaG"
                     /locus_tag="PKN31_04515"
     CDS             2293..4047
                     /gene="dnaG"
                     /locus_tag="PKN31_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOA99223.1"
                     /translation="MLFDDQYLEEIKSRIDIVELVSRYVSLQPAGRNYRALCPFHEEK
                     TPSFLVSPEKGIFHCFGCGVGGNIFTFVMKMESLTFPEAVAFLAEKCGMELPTLGKSI
                     TRQTKERDKLFLLNETLNRYYYQCLLNPGDELAHAARSYIYERRGLKPEIIEQFGIGF
                     SPASGKESIAFLMEAGFSGQDVIKAGVGVVTKSGELLDRFRGRITFALHDVQGRVIGF
                     GGRILGDGEPKYVNVSDTPVFSKGRNLYGLFASRNFIQRSHQAILVEGYMDFLTLFQE
                     GINNCVASMGTSLTKDQAMLLRRYAEEVIICYDSDAAGQAATVRGMNILQEQGITVRI
                     LPIPAPYDPDSFLRQKGREKFLEIMEEAPSLFDFQLELFVKDYGLSSLESKAKIIRGM
                     VPFLQSIRDPVERSLKVSYLAERLKVGESVIYSALRREKAPEAVLSSFVSRESGKVKA
                     EKMLLRAMMESQFWRETVVKELEVQNFSRPEHRRIFRVFADSEEEGNLSPSDLIDIFS
                     GDEEISCCITEIMTREDLQGGLNQEVVEDLIRRLKLASVEEEMQRIQEELSREDNEER
                     LQYYHFLSQEREKLKRLD"
     gene            4059..5156
                     /gene="rpoD"
                     /locus_tag="PKN31_04520"
     CDS             4059..5156
                     /gene="rpoD"
                     /locus_tag="PKN31_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOA99224.1"
                     /translation="MWLSGDNKNKSSPEVSSLMERGKQKGYITFKELNDALGSEAINV
                     DKLDDVYNTLLGLGIEVSDEEGFQEDQPTMEAEDFHFEDIKGIGADDPVKMYLKEIGQ
                     VPLLTKEEEVELAKRVEQGDPEAKRKLIEANLRLVVSIAKKYVGRGMLFLDLIQEGNL
                     GLIRAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLNRTSR
                     QLVQELGRDPTPEEIAEKMGITPEKVREIMKTAQEPLSLETPIGEEEDSHLGDFIEDT
                     GALAPPQAASYSLLKEQLDNVLNTLTDRERRVLKLRFGLEDGRPHTLEEVGQIFGVTR
                     ERIRQIEAKALRKLRHPSRSKKLRDYLDETE"
     gene            5184..5258
                     /locus_tag="PKN31_04525"
     tRNA            5184..5258
                     /locus_tag="PKN31_04525"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5216..5218,aa:Asn,seq:gtt)
     gene            5607..6713
                     /gene="ugpC"
                     /locus_tag="PKN31_04530"
     CDS             5607..6713
                     /gene="ugpC"
                     /locus_tag="PKN31_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOA99225.1"
                     /translation="MANVSLKSVVKRFGEVVAVNKVDLDIADEEFLVLVGPSGCGKTT
                     TLRMIAGLEEVDEGEIYIDDILVNDVPPKDRDIAMVFQNYALYPHMDVYNNMAFGLKL
                     RKFPKDEIDRRVKEAADILGIHELLNRKPKQLSGGQRQRVAVGRAIVRHPKVFLFDEP
                     LSNLDAKLRVQMRAELSKLHDRLKATMIYVTHDQIEAMTMGDRIVVMKDGIVQQVGTP
                     LDLYNKPQNKFVAGFIGTPSMNFLDVVIKKEGDALYLDGESFKIKIPAEIQSALLPYV
                     NKEVTLGIRPQDIYDCNFSQEMAEVDGNVIETTVDIVEPLGSEQTVYLVTGPHVLVAQ
                     LDMRTHTSVGDTLKILVDTSKIHLFDVETEQAIV"
     gene            7054..8043
                     /gene="gap"
                     /locus_tag="PKN31_04535"
     CDS             7054..8043
                     /gene="gap"
                     /locus_tag="PKN31_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOA99226.1"
                     /translation="MKVGINGFGRIGRLVFRRILEEGDLDVVAVNDLTSAKTLAHLLK
                     YDSVHGTICNQVEAKDDALVVDGKEIKVLAQKDPAQLPWRDLGVDLVVESTGKFTARD
                     GASLHLQAGAQKVIITAPAKGPDITVVMGVNHKAYNKDAHHIISNASCTTNCLAPVVK
                     VLNDSFTIQKGFMTTIHAYTNDQVILDFPHKDLRRARAAALSMIPTTTGAASAIGEVI
                     PELKGKLDGMAIRVPTPDVSIVDFVAVVEKETSIEEVNSVLKQAAEGELKGILAYCDE
                     PLVSVDFLHDTHSSIVDALSTKVHGNLVKVLAWYDNEWGYSCRVVDLVKYVMK"
     gene            8055..8825
                     /gene="tpiA"
                     /locus_tag="PKN31_04540"
     CDS             8055..8825
                     /gene="tpiA"
                     /locus_tag="PKN31_04540"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOA99227.1"
                     /translation="MPRIPIAAGNWKMNKTSQEASAYAEAFLPQIKDFKKEVEIIICP
                     PFTALSALASYFRNSPVKLGAQNMHWEEKGAFTGEVSAPMIKEWGCQYVILGHSERRH
                     IFRETSEEVGKKVKKALEWGINPILCVGETVEEREKGETEKIVEEQLLLGIADIPDTK
                     ASSIVIAYEPVWAIGTGKAATPEDAREVISFIREKLLSRFSSEISDNIRILYGGSVSP
                     ENVFNFLREEEIDGTLVGGASLDPLKMIKIVEETLRAY"
     gene            8844..9068
                     /gene="secG"
                     /locus_tag="PKN31_04545"
     CDS             8844..9068
                     /gene="secG"
                     /locus_tag="PKN31_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOA99228.1"
                     /translation="MEWWMIVQIIICVALILVVMFQPRKAGMGGGMFGGMTRADRSSK
                     FRSLPVLSKLTVILAAAFLIFSFLFSFLLT"
     gene            9081..10121
                     /locus_tag="PKN31_04550"
     CDS             9081..10121
                     /locus_tag="PKN31_04550"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOA99229.1"
                     /translation="MIENNGILTLKEVENIKIDPERDFYSATPEEIKKGYTTDIYFVS
                     AQEILHHLDLKDTIVTAEIFPRGSGVMCGVPEVLNLLSSSSVEIWSLSEGEEFSPKEV
                     IMRIRGPYSAFGMFETPLLGILAHSSGWATAARECKVAAGEKIVLSFGARHVHPAVAP
                     VMERSAIVGGADGAACILGAKLAGKEPGGTIPHAAIIIIGDTVVAAQAFQEIIPPHYS
                     RVILVDTFKDEAEESLRVARALGENLEGVRLDTPEERGGVTPGLVEEVRVRLDQAGFS
                     QVKIVVSGGLNPARIQALKEKVDIFGVGSYISGHSPVDMTMDLKEIGGEKIAKRGRLP
                     GIIPNPRLKQIK"
     gene            10138..>10328
                     /locus_tag="PKN31_04555"
     CDS             10138..>10328
                     /locus_tag="PKN31_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99230.1"
                     /translation="MKKYGVLLLAVLVLIGGVSFAEVKNPDTYIHLGIGEPETLDPHY
                     AYDTASGEALGMVYDNLIA"
BASE COUNT         2812 a         1836 c         2642 g         2938 t
ORIGIN      
        1 atttatgctg tttttctcgt tcgccggatc tattcttttg acccgggaaa tgagaaaatg
       61 cagagtttgt ccagtgcaat tcaggaagga gcgatggcgt tcttgaagag agaatattcc
      121 ttcctggcta tctttattat tggtatgttt atacttctta ctctaactct gggggtgacg
      181 gtagctatct gttatgtgtg tggtgctatc tgttctgtgg gcgccggctt tgtaggtatg
      241 agagtggcca ctcgcagtaa ctgtcgcact actcaggcag ctacaaaaag cataagcgat
      301 gctctgacag ttgctttttc ctcaggagca gttatgggga tgtgtgtggt gggatttggt
      361 cttgtgggac tgagcctcct ttaccttttt gtgggagacc ctgatattat caacggtttt
      421 gctttaggag ccagctctgt tgctctcttt gcccgggtag gagggggtat ttacaccaaa
      481 gctgctgatg tgggagcaga tctggtgggc aaagtggaag cgggtattcc cgaggatgac
      541 cctcgcaacc cggcagttat tgccgacaac gtaggtgaca atgtagggga cgtagctgga
      601 atgggagctg acctttttga atcttatgtg ggctctatta tagctgctat ggtgctggga
      661 gcagcaccgg gggccatata tggaggaaca agcgggatag tatttcctct tcttcttgct
      721 ggtctgggag tggtggcttc tattatcgga actttatttg ttcgagcaaa agacgaaaaa
      781 gatttagcac ctgctctcaa ccggggaacc tacatcagtg ctgttttaac tgctattttt
      841 gtcttgattt ctgcccttct tgtttttcga gacctgggaa cagcaaaagc cattggtcca
      901 tttgtggcca gcgtatcggg tctgatagcc ggcattatta ttggtcttct ctgtcaatac
      961 tatacctcca gtgattataa accggtgcag caactggcag agacggctaa aactggtccg
     1021 gctacggtga taattaatgg tttggctctg ggtatggaaa gcactgtttt tccccttatt
     1081 ttaatttgcg ctgctattct tctggctaac tgggcagcag gaatttacgg tattgccata
     1141 gcgggtttgg gcatgctctc tattgtaggc atgacggtgg cagtagatgc ctatggtccg
     1201 gtggctgaca acgccggagg tattgccgaa atggctgatt tagatcctca tgtgcgtgaa
     1261 agaaccgatt ccctggatgc tctgggaaat accacagcag ctatcggtaa aggttttgcc
     1321 attgcctctg cagctctcac cgctcttgcc ctgtttgtgg cttattcgca aactgtgggt
     1381 ttgaggatta ttgacctctt gcatccctac acagtggtgg ggcttttcat tggaggaatg
     1441 atgcccttcc tcttttcctc cattgctatg aaagcagtgg gaactgctgc ttttaaaatg
     1501 gtggaagaag tgcggcgtca attccgggaa ataaaaggat tgatggaagg gaaagctcaa
     1561 cctgattatg ctcgttgtgt agatatcagc actcgagggg ccattgccca gatgatcttt
     1621 cccggtcttt tagcggtgat agtgcctatt gtggtgggtc tggttttagg taaagaggca
     1681 ttagggggat tgctggccgg agcaattgtt accgggcttc ttatggccat tatgatggcc
     1741 aatgctggag gagcctggga taatgccaaa aaatacatag aagcggggaa cttgggagga
     1801 aaaggaactc ctgtccatgc tgcagcagta gtgggagaca cagtgggtga tccttttaaa
     1861 gataccgccg gtccttctct taacatactg attaagctta tgtctatcgt agctttagtt
     1921 tttgctcctc ttttcatgcg ctaaatattc tttctcctcc gggaatattc ccggaggaga
     1981 aagaatttaa ttaaaggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     2101 gtacattccg gtaagaatgc atttttcctc aggaggggtc tggtaaagaa atacttgatt
     2161 ccgggtgttc tgtcaggggt gtttttcgaa ttagaagtac atagataatc gggaatattg
     2221 ccctgaatgg gcaacattct atcgctaaca ttattattgt tcttcccgga tgaggaggaa
     2281 taggtgattt aattgctgtt tgatgatcag tatctggaag aaattaaaag tcgtatagac
     2341 atagtggagc tcgtttcccg gtatgtcagt ctccagccag caggtcgtaa ctatcgtgct
     2401 ctttgtccct ttcatgaaga aaagactcct tcctttctgg tttctccgga aaagggcatt
     2461 ttccactgct ttggatgcgg ggtgggtggt aatattttta cctttgtaat gaaaatggaa
     2521 agcctcacct ttcctgaagc ggtggctttt ttagctgaaa agtgtggcat ggagctaccg
     2581 actctcggta aaagtatcac ccgacaaact aaagagagag acaaactttt cctcttgaat
     2641 gaaactttaa atcgttatta ttatcaatgt ttgttgaacc ccggggacga acttgctcac
     2701 gctgctcgct cttatattta tgaacgcagg gggctaaagc cagaaataat agagcagttt
     2761 ggcattggtt tttctccagc ttcaggtaag gaatccattg cttttttgat ggaggcaggg
     2821 ttttcgggac aggacgtaat taaagctgga gtgggagtgg taacaaaaag cggggaactt
     2881 ttagaccgtt ttcgaggtag aattactttt gccttacatg atgttcaagg gagagtaatc
     2941 ggttttggag gaagaatttt aggggatggg gagcctaagt atgtcaatgt ttctgatacc
     3001 ccggtttttt ctaaaggacg caacctctac ggtctttttg cctcgcggaa ttttattcag
     3061 cgctctcatc aggcgatttt agtagaggga tatatggatt ttcttactct ttttcaagag
     3121 ggaattaata attgcgtggc ttctatgggt acttctctta ctaaagatca ggccatgctc
     3181 ttgcgtcgct atgctgaaga ggtaattatt tgttacgatt cagatgcggc aggacaggcg
     3241 gctactgtga gaggcatgaa tatccttcag gaacagggaa taacagtgcg aatactgcct
     3301 attcctgccc cttatgaccc tgattctttc ctacgtcaga aagggagaga aaaattctta
     3361 gaaattatgg aagaagctcc ctctcttttt gatttccaac tggaattatt tgttaaggat
     3421 tatggtttaa gttctttgga atcaaaagcg aagattattc gaggcatggt tccttttctc
     3481 caatcaattc gagatccagt agagaggtct ttaaaggtta gctatttagc agagaggtta
     3541 aaagtgggag aatcagtgat ttatagtgct ttgagacggg aaaaagctcc tgaggcagtt
     3601 ttaagttctt ttgtgagcag ggaatcgggt aaagtaaaag cagagaaaat gctcctgcga
     3661 gctatgatgg aaagtcagtt ctggcgggag acagttgtca aagaactgga agttcaaaat
     3721 ttttctcgac ctgagcatcg tcgcattttt cgtgtttttg ctgattctga agaggagggt
     3781 aacctttctc cttctgattt gatagacatt ttttctggcg atgaagaaat atcttgctgt
     3841 ataacagaga taatgacccg ggaggatttg caaggaggat tgaatcaaga agtggttgag
     3901 gatttaatac gccggttgaa gctggcttca gtagaagagg aaatgcaacg gatacaggaa
     3961 gagctttctc gagaagataa cgaagagagg ctacagtatt atcattttct ctcgcaagag
     4021 agggaaaaat tgaaaaggtt agattgaaga gaggagagat gtggttgagc ggcgataaca
     4081 aaaataagtc ttcccccgaa gtttcttctt taatggagag aggaaagcag aaagggtata
     4141 ttacttttaa agagttaaat gatgccctgg gtagtgaagc cataaacgta gataagctgg
     4201 atgatgttta taataccctt ttgggtctgg gtattgaggt ttcagatgag gaaggctttc
     4261 aggaagatca acctaccatg gaagcggaag attttcactt tgaggatata aaaggcatag
     4321 gagcagatga cccggtaaaa atgtatctga aagaaatcgg acaggttcct ctcctcacca
     4381 aagaagaaga agtagagtta gcgaaaagag tggagcaagg agacccggaa gccaaaagga
     4441 agctcatcga agccaatctg cggctggtag tgagtattgc taaaaaatac gtgggcagag
     4501 gcatgctctt tcttgacctc attcaggagg gtaatctggg cttaattcgg gcagtagaaa
     4561 aatttgatta tcggaagggt tataaattca gtacttacgc tacctggtgg attcgccagg
     4621 ctattacccg ggctattgct gatcaagcgc gaactattcg tatccctgtc catatggtag
     4681 aaaccattaa taaactcaat cgaacttctc gacaactcgt tcaagagctg ggaagagacc
     4741 ccacacctga agagatagca gagaagatgg gtattactcc ggagaaagtc agagaaataa
     4801 tgaagacagc ccaggagcct ttgtctctgg aaacccctat tggagaggaa gaagacagtc
     4861 acctgggtga ttttattgaa gatactggag ctctcgcgcc tcctcaggct gcctcttatt
     4921 ctcttttgaa agagcagtta gataatgttt taaatactct tacagaccgg gagaggagag
     4981 ttttgaagct ccgttttggc ctcgaggatg gaagacccca tactctggaa gaggtaggac
     5041 agattttcgg ggtaactcga gaaagaattc gtcagattga agccaaggct ttgagaaaac
     5101 tccgccatcc cagcaggagc aaaaaattgc gtgactatct tgacgaaaca gaataagttt
     5161 cctataattc tctgtgaagc cgctcctcgg tagctcaatg gtggagcatc cggctgttaa
     5221 ccggagggtt gcaggttcga atcctgcccg gggagccaga ttgacaattg attggtggtt
     5281 gtttaaagct tttctgaagc attgcctcat ctttcccgca caatgcccga aaaaaataag
     5341 gaaaactgag aatctttttt ctcctgtcta tccctgaatg gccagttacc agttattttt
     5401 aactaaagac tttcccgtgg ttcttctatt cagatatgat agtatttctg tctgggggaa
     5461 gagaaataga gtaaaaaata aaaataccct gcatcaggac actatcttta acttttaagg
     5521 ggttgcaaga ggaaagcaaa tattctaaaa tagagtaagc atttctttat tacttaaatt
     5581 taagtgaatg aagaggagga aaataaatgg ctaatgtttc tttaaagagt gtggttaaac
     5641 gttttgggga agtagtagca gtgaacaagg tggaccttga tattgcagat gaagaatttc
     5701 tggtattagt aggtccttct ggttgtggta aaactactac cttgcgcatg attgccgggt
     5761 tggaagaagt ggatgaaggg gagatttata ttgatgatat cctggtaaac gatgttcccc
     5821 ctaaagatcg ggatattgcc atggttttcc agaactatgc tctttacccc catatggatg
     5881 tgtacaacaa tatggctttt gggttgaagc tccgtaaatt tcccaaagat gaaattgata
     5941 gacgggtaaa agaagcagca gatattctgg gaattcacga gttgcttaac cgtaaaccta
     6001 agcaactctc tggaggtcag aggcagagag tagctgtggg aagagccatt gttcgccacc
     6061 ccaaagtctt cctgtttgac gagccccttt ctaacctgga tgctaaattg agagtgcaga
     6121 tgagagcgga gttgtctaaa cttcacgacc gtctgaaggc caccatgatt tatgttactc
     6181 acgaccagat agaagctatg actatgggag atagaattgt agttatgaag gacggtatag
     6241 tacaacaagt gggtactcct cttgatctct acaataagcc ccagaataaa tttgtggctg
     6301 gttttattgg taccccctcc atgaatttcc tggacgtggt gataaagaaa gagggagatg
     6361 cactttattt agatggagaa tccttcaaaa tcaaaattcc ggctgaaatt caatctgctc
     6421 tcttgcccta cgtaaataag gaagtcactt tgggcattcg tccccaggat atttatgatt
     6481 gtaacttctc ccaggagatg gcagaagttg atggaaacgt gatcgagacg acagtagata
     6541 tagtggagcc tctgggttca gagcaaacag tgtatctggt taccgggcct cacgttcttg
     6601 ttgcgcaatt agatatgcgt actcatacca gtgtgggaga tactttgaaa atactggtgg
     6661 acacctctaa aattcatcta tttgatgtag agacggagca agctatagtt taagttgccc
     6721 tgtcaaagca agagtaggtg agagttgcat tgtggctcct ccgtttttat aataaggggc
     6781 tggagggtaa aaaagtgcgg gttatacccc ggaaaggaaa ttgccttttt gaagaaggct
     6841 tatcgggatg atgagaaaat gcaggatgga ggatgtctta cctctatcgc tggaagcagt
     6901 gaaattataa tctgtagata tactgaaacg tgagaaagaa ataattgttt tagatacccc
     6961 cgtccgcgag acgggggtat ctactgaaaa gaatgcttga gcattctttc tctacactga
     7021 ataaatagta ttcctaagaa aggagcagcg attatgaaag ttggaatcaa tgggtttggt
     7081 agaattggac gtttagtttt tagaaggatc ttagaagagg gagacttaga cgtagtagca
     7141 gttaatgatt tgaccagcgc taaaacttta gctcacttgt taaaatatga ttcggtgcat
     7201 ggaactatat gtaaccaggt ggaagctaaa gatgatgctt tagtagtaga tggtaaggaa
     7261 atcaaagttc tggctcaaaa agaccctgct caattgccct ggcgggactt gggggtggat
     7321 ctggtagttg agtctactgg taaatttacc gccagagacg gagcctcttt gcatttacaa
     7381 gctggtgctc agaaagtaat aatcactgct ccagctaaag gaccagatat caccgtagta
     7441 atgggagtta accataaagc ttacaacaaa gatgcccatc atattatttc taacgcttct
     7501 tgcaccacca actgtctggc tccggtggtt aaggttttga atgatagctt taccattcag
     7561 aaaggtttta tgaccactat tcacgcttat accaatgatc aggttatttt ggactttcct
     7621 cataaagact taagaagggc gcgggcagca gctctatcta tgattcctac cactactgga
     7681 gcagcttcgg ctatcggaga ggttatacct gagcttaagg gcaaattgga cggaatggcc
     7741 attcgagttc ccactcctga tgtctccata gtggactttg tagcggtagt ggaaaaggaa
     7801 acctctatcg aggaggtaaa ttccgttctc aaacaagctg ctgaaggtga attgaaaggg
     7861 attctggctt actgtgatga gcctttagta tcggtggact ttctccatga tactcattct
     7921 tccatcgtgg atgctctgtc taccaaagtt catgggaacc tggttaaggt tttagcctgg
     7981 tatgacaatg agtggggtta ttcctgtcgg gtagtagatt tagttaaata cgtgatgaag
     8041 taggagttat agaaatgcct cgtattccta tcgctgcagg aaattggaaa atgaataaaa
     8101 cttctcagga agcttcagcg tatgctgaag ctttcctccc ccagataaaa gacttcaaaa
     8161 aggaagtaga aataatcatt tgtcctcctt ttactgctct ttcagctctg gcttcttatt
     8221 ttcggaattc tccggtgaaa ttaggagcac aaaatatgca ctgggaggag aaaggtgctt
     8281 ttactggtga agtgagtgct cctatgatta aagaatgggg ttgtcagtat gtcattctgg
     8341 ggcattcaga gcggaggcat atttttcggg aaacctctga agaagtgggt aaaaaagtca
     8401 aaaaagcatt agaatggggt attaacccta ttctttgtgt gggggaaact gttgaagaga
     8461 gagaaaaggg agaaactgag aaaatagtag aagagcaact acttctgggg atagcagaca
     8521 ttcccgatac caaagcatcc tctattgtga tagcctatga accggtctgg gctattggta
     8581 cgggaaaagc ggctactccc gaagatgctc gggaggttat ctcttttatt cgagaaaagc
     8641 ttttatctcg ttttagctct gagatttcag ataatattcg cattctctat ggaggcagtg
     8701 taagtccaga aaatgtattc aattttcttc gagaggaaga aattgatgga accctggtag
     8761 gaggagccag cttagacccg cttaaaatga ttaaaatagt agaagaaact ctgcgagcct
     8821 attagagaga aagaggtcgg aatatggaat ggtggatgat tgttcaaatt ataatctgtg
     8881 tggctctgat tttagtagtt atgtttcaac cccgcaaagc aggtatgggg ggtggaatgt
     8941 ttggcggtat gacgagagca gaccgaagca gtaagtttcg gagtttacct gttttgagca
     9001 aattgactgt aattctggca gcggcgtttc tgattttttc ttttttgttt tcctttctgt
     9061 taacttaata aaggaccaag atgatagaga ataatggaat attaactctg aaagaagtag
     9121 agaatatcaa aatagacccg gagagggatt tttactctgc tactccggag gaaataaaaa
     9181 agggctatac tactgatata tattttgtca gtgctcagga gattttacat cacctggatt
     9241 tgaaagatac catagtaact gcagaaattt ttcctcgagg gtcaggagta atgtgtggag
     9301 tgccggaagt ccttaatctc ctctcttctt cctcggtaga gatctggagt ctgagtgaag
     9361 gggaggaatt cagtcctaaa gaggtgataa tgaggatcag gggaccttac tcggctttcg
     9421 ggatgtttga aacccctctt ctgggaattc tggctcacag ttctggctgg gctactgctg
     9481 ctcgggagtg taaagtggca gcaggagaaa agatagttct ttcctttggt gcccgacatg
     9541 ttcacccggc tgtggctcca gttatggaac gctctgctat tgtaggagga gcagatggag
     9601 cagcgtgtat actaggagca aagctcgcgg gaaaagaacc gggaggtact attcctcatg
     9661 ccgcaattat cattataggg gatacagtgg tagcggcgca ggcttttcaa gagattattc
     9721 cccctcatta ttcccgggta attctggttg atacctttaa agatgaagct gaagaatctt
     9781 tgcgagtggc tcgagcttta ggagaaaatt tagagggagt gcgactggat actcctgagg
     9841 agcggggagg agtaactccg ggtcttgtag aagaagtgag agtgcgctta gatcaggctg
     9901 gtttttctca ggtgaaaatt gtagtctccg ggggtttgaa ccctgcaaga atccaggcgt
     9961 tgaaagaaaa ggtagatatt tttggagtgg gaagttatat cagcggtcat tctcctgtgg
    10021 atatgactat ggatttaaaa gagataggag gagaaaaaat agccaaacgg gggaggttgc
    10081 cggggattat tcctaatcct cggttaaagc aaataaaata aaaaggaggc gttagctttg
    10141 aaaaagtacg gtgttcttct tctggcggtt ttagttttaa taggaggagt gagttttgcc
    10201 gaagttaaaa atcccgatac ttatattcac ctgggtatcg gggaaccaga aacattagat
    10261 cctcactatg cctacgatac ggcaagtggc gaggcattgg gaatggttta tgataacctg
    10321 attgctta
//
LOCUS       DAOPSS010000053        10155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_5551_length_10155_cov_8.439901, whole genome shotgun sequence.
ACCESSION   DAOPSS010000053 DAOPSS010000000
VERSION     DAOPSS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5551_length_10155_cov_8.439901"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..949
                     /locus_tag="PKN31_04560"
     CDS             44..949
                     /locus_tag="PKN31_04560"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HOA99231.1"
                     /translation="MLFIISSLVVLTFIFPVKLNCPSHLHLQAGGEEKVEEIEPLTLW
                     GFPSWFQRATVAIEDKELIFASQEGKEVIFQPRRLEGKTSVLVGVFPVWRKIEVEILP
                     NREDRDGDGFPDMAELSAESERDAFREFFVKVARSQIVSPHPQFKERDCGGLVRFAYR
                     ESLKRGDIIPYTYPRVPLLGVNLFRIKPGSFNPEKIEEDFSVFASSFYLLAHNVVPLG
                     KEEGKAKAGDLLFFFQPVSFSYPYHVMIYEGEGRVIYHTGPIEEGEGVRETTITSLRK
                     HPDINWHPTKDNPNFLGFFRWKILD"
     gene            962..5374
                     /locus_tag="PKN31_04565"
     CDS             962..5374
                     /locus_tag="PKN31_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOA99232.1"
                     /translation="MKKNKIVFWWVTVFLLLLVGEALAQEEIYVSVPAHIYPGEMIQI
                     NISGREIKGAQCSLYQINDPEEYIQSAEKKSFFQVDSWQLRRPLEKEEKWQNTKYSFF
                     QAVRRLASRVLPVSVKEYLRDLFDIHGPLPRRPIAITPSDFRRGTDELKTFWVDLPQE
                     KDSPFFWREVEINPLPPGVYLFTAQGGGKEARVLFSVTHLSCFSRHDREKGVILVQDL
                     KTGIPQEGVKIKIWQGEEREEGFTNNQGLYSWKREWKEGEEAVIMVEKDNEFAFLNFY
                     PYYWEGGKLKAFIYSERPLYKPGQTVLGKAILREVSTDKYIVPPPGEKVEVALVDYRD
                     NEYGKESLLSTPEGSVNFSFSLPQKIEEGYFLIRLRWRDREFYQVISIENYEKPVFTV
                     QLEPEKPVYARGEEVCFSLKGEYYSQRPLEGGEFRYQIFRYPVGYWEKERGEEMWGEG
                     EGKLDEKGEGEIRFLPEGDGAFRYRVHCVVFDPAYRAVEKEEEVQVLMGEFYLSLQPR
                     VYFVKTGETMTYEVEARDAEEKKVPVGKVYGEVYRLEWEKETEKWREIPSQFLDISLP
                     EGTGEMKITLDRAGYYRLEVYTRDRSGNKILASSYFWVTGKDLHYPSRELEVRWDRER
                     YRVGEEARVLIVPPGEGTFTFLFSIEGSELQEAEVKQISAPEEIVIPVLPSYVMGVYV
                     SFSGFSNGKLYSESLSLPLEREKELQIKITPDRERYQPEEKGKISIQVSDAQGKPVKC
                     ELSFAIVDQAIFDLSSWWRENVYDYFYSQFYNLVYGSYSIYSSFYGMGIPFQDMKAQL
                     KGMGGAEGGLLLRKYFPDTLLWRPTLSTDEEGKAEVDFVAPHSLTRWRVEVKAHQGEF
                     FGEAQEYFTTYQPFALGVELPSFLVEGDILKSKATLWNEVAPQEVVIEGESSPEISLE
                     WKREPLKVSLLEEAVFPLEVKAQYPGKAYLRLFARGEYAQDAIELPLEIKERGVEVEE
                     IFYSFLEEKKTSFEFSCDSSSSVELALFSDPRSILLEEIEYMLSYPYRCSEQVASRLI
                     TFSSLPLGKMEDKILEVTEAIREDIYSLYNLQSYDGGWGFWGGEGDLFHTAYTLLALH
                     KVKERGFLVNAEVIERGLSFLTQEISRSEAPSSDSTFSPPSEFDRALSWYALSLWKKD
                     FVPSPLSPLEEMDDKTLALRGLVAREEDKEKVEKELEKRAHVLGDTVFWGTINPQKPW
                     EDDRFPATAFAALFLAERGNPLGEKAYLWLWRSKKGGNLFTTLNRAYFFLVSSHFLKE
                     ESGVPTFRVFCNGALVKEGKEEEKIAIPRQFLKEGSNHLEVEGDNLFMTELKVKSWKK
                     EAPSSHFFSVKKNYYLLVPTKGEKVFFSPQKVDAFHSQEEVVCEIEIQSPDYLEYLII
                     EDGMPAGCELIRKDYTFSLPEEEGAHYPGVIKTLFEGQPTLFVYELFPGKNVIRYYLR
                     IRDQGNFYVKAPLLYLMYFPEVRDYGEEKNLVVE"
     gene            5343..5993
                     /locus_tag="PKN31_04570"
     CDS             5343..5993
                     /locus_tag="PKN31_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99233.1"
                     /translation="MEKKRIWWWNNSCIRSFFLGVVIILFLSQRGGALPAFVIEGGES
                     FPFLPPIVESSYKEAQLCFQRVTGYSPCSSFRIKVLSPEISNTLPSWVAAFSRGSTIY
                     LRSPLLLFQRGILSSTLAHEFFHVFVESFGWQLPSWFEEGMASFFFPTPGEIFPEGAG
                     EFYEGCQESFRIFKEKIGEENLPSFFSTVQEMGFEEAFFIWTGEKEEDFGKHIDGE"
     gene            5983..6699
                     /locus_tag="PKN31_04575"
     CDS             5983..6699
                     /locus_tag="PKN31_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99234.1"
                     /translation="MENDVILKFKNVGKKIRDRVILEGVSFEVREGEIFGLLGGNGAG
                     KSTLIRLLFGLVLPSAGYFYVGDYRCPPHNPEAKALMGGCLDNTSFYGNLTGIENITL
                     FASLSSCYPRRGEIEYFASLLGFEPSLLYKKVSGYSQGEKKKLSLLHSLFPLPRILVW
                     DEPWVGLDPRGAKEVQELILALNAEERVTFFLSSPVSQEVEKIAQRAAILHQGKIKWN
                     DYLTPNVSLEEIYWEIIERK"
     gene            6696..7421
                     /locus_tag="PKN31_04580"
     CDS             6696..7421
                     /locus_tag="PKN31_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99235.1"
                     /translation="MKAIKAEIKKAIHNPRFYGGILISWALAGLVVAQAWRDPLSFYL
                     GKITLSFSLLYPLALFGPVGGIWGGTENIAEERMEGTLSTTLLRPLSRWEWYWGKAGF
                     TLFYNLLLVFTPLLGMALLEKIMWGGGFSFRLLGGGVMVALYISSFSALSYLFSSLFY
                     RPWGGPLLALGIGAFSWIVERLGGGENVQIYLITHHLSSFNIFFLPRIDWHQALSSFL
                     ILGSYTGGGLLIGALIFMRQEEK"
     gene            complement(7738..8868)
                     /locus_tag="PKN31_04585"
     CDS             complement(7738..8868)
                     /locus_tag="PKN31_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA99236.1"
                     /translation="MQYRPFGKLDFQVSALGFGAMRLPSKSADYTDIDEAQALEMIHY
                     AIDHGVNYLDTAYGYHGGKSEILVGKALKGGYRQKVKVATKLPTWLVNAAPDMDRYLD
                     EQLRKLDTEHIDFYLFHDLNKDRWAKLERLEALNWAEKAIQKGKIGHIGFSFHDDRET
                     FKKIIDAYPWTFCQIQYNYLDIDHQAGQFGLQYAAQKGIAVVIMEPLRGGRLANPPEE
                     VQKIFDEAPVKRSPVAWALLWLWNQKEVAVVLSGMSNLQQVKENIEIASSSGVGLLSP
                     EELKIIERAREAFERLPSINCNACKYCLPCPNGVNISRNFELYNDAFQKIAFEDARRK
                     YLALPEEERASSCLGCGDCEDLCPQELPIPELLEKVARAFEE"
     gene            9037..9531
                     /locus_tag="PKN31_04590"
     CDS             9037..9531
                     /locus_tag="PKN31_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA99237.1"
                     /translation="MIEYLTDLEKRDLLDVRELLIEYADWLNLDLSFQGFSEEIETLP
                     GRYSPPEGALILARVDGVAAGCVALRKLSSLVCEMKRLFVRPSYRGMGIGKQLVARII
                     EEAIKRHYQFMRLDTLPLMKDARKIYQDMGFYPISPYYDNPIVGAEFMELNLENWSKK
                     GTRL"
     gene            complement(9965..10040)
                     /locus_tag="PKN31_04595"
     tRNA            complement(9965..10040)
                     /locus_tag="PKN31_04595"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10005..10007),aa:Lys,seq:ctt)
BASE COUNT         2764 a         2013 c         2384 g         2994 t
ORIGIN      
        1 gggaagcaat tttagaatag agggatgaaa tacgaaaaaa atattgcttt ttatcatttc
       61 cagcctggtg gttttaactt ttatctttcc tgttaaactc aactgccctt ctcacctgca
      121 tcttcaggca ggaggagagg aaaaagttga ggaaatagaa cctcttactt tgtggggttt
      181 tccttcctgg ttccagagag ccacagtagc gatagaagat aaagaattaa tttttgcaag
      241 tcaagaggga aaggaagtta tctttcagcc ccggcgtctg gaggggaaaa cttcagtatt
      301 agtgggagtg ttcccggttt ggcgaaaaat agaggtggag attctcccca accgggaaga
      361 tagagatgga gatggtttcc ccgatatggc ggagttaagt gctgaaagtg aacgagatgc
      421 ctttcgagag ttttttgtta aagtggctcg ctcgcaaatt gtctctcctc atcctcaatt
      481 taaggaaaga gattgtggag ggctggtccg ttttgcctat cgagagagcc taaaaagagg
      541 agatatcatt ccttacactt accctcgagt tcccttactg ggagtcaacc ttttccggat
      601 taaaccgggc agctttaacc ctgagaaaat agaagaagat ttttcggtct ttgcctcttc
      661 tttttacctt ctggctcata atgtagtgcc tctgggaaaa gaggaaggaa aggcaaaggc
      721 gggagacctt ctctttttct ttcaacccgt ctctttttcc tatccttacc atgttatgat
      781 ttacgaagga gaaggaagag taatttacca caccggtccc atagaagaag gagaaggagt
      841 gcgagagacc actattacct ctttgcgcaa gcatcccgat ataaactggc atcccacaaa
      901 ggataatcct aactttctgg gatttttccg ctggaaaatt ttagattaga ggggagaaaa
      961 gatgaaaaaa aataaaattg ttttttggtg ggtaacagtt tttctactcc ttttggtggg
     1021 agaagcttta gcccaggagg aaatctatgt tagtgtccca gctcacatct accccggaga
     1081 aatgatacag ataaacataa gtggtcgaga gataaaagga gcacagtgtt ccctttacca
     1141 aatcaatgac ccggaggagt atatccagag cgcagaaaaa aaatctttct ttcaggtaga
     1201 tagctggcaa ctacgccgcc ctctggaaaa agaggaaaaa tggcagaaca ccaaatattc
     1261 cttctttcag gcagtgcgaa ggcttgcttc ccgggtactc cctgtctcag tgaaagaata
     1321 tttgcgtgac ctgtttgata ttcacgggcc cttgcctcgc cgccccattg ctatcacccc
     1381 ttctgatttt cgccggggga ctgatgagct aaaaactttc tgggtggatt taccacagga
     1441 gaaagattcc ccctttttct ggagagaggt ggaaattaac cccctgcctc ccggggtgta
     1501 cttattcact gctcagggag gaggaaaaga agcccgggtt cttttcagcg taacccatct
     1561 atcttgtttt tcccgccatg acagagaaaa aggcgttatc ctggtgcaag acttaaaaac
     1621 tggtatcccc caggaaggag taaaaattaa aatctggcag ggtgaggaaa gagaggaagg
     1681 gtttaccaac aatcagggat tgtattcctg gaagagagag tggaaagagg gagaagaagc
     1741 agttattatg gtagaaaaag ataatgaatt tgcctttttg aatttttatc cttactactg
     1801 ggaaggtgga aaattaaaag ctttcatcta ttccgagcgt cctctgtata agccaggaca
     1861 aactgttctg ggaaaagcta ttttacgaga agtcagtacc gataaatata tcgttcctcc
     1921 cccgggagaa aaagtggaag tggctctggt agattatcga gacaatgagt acggaaaaga
     1981 aagcctcctt tccactcccg aggggagtgt gaacttttcc ttttccctgc ctcagaaaat
     2041 tgaagaagga tatttcttaa tccggttgcg ctggcgagat agagaatttt atcaggtgat
     2101 ttccattgag aattatgaaa agccagtttt tactgtccag ttagagccgg aaaagccagt
     2161 ttatgcccgg ggagaagaag tttgtttttc actgaaagga gagtattatt cccagcgccc
     2221 tttagagggg ggtgaattcc gctatcagat attccgctat cctgttggct actgggaaaa
     2281 agaaagagga gaggaaatgt ggggagaggg agagggaaaa ttagacgaaa aaggagaggg
     2341 agagatacgg tttcttccgg agggagatgg agctttccgt taccgggtgc actgtgtggt
     2401 gtttgacccc gcttatcgag cggtagaaaa ggaagaagag gttcaagtct tgatgggaga
     2461 gttttatctt tccttacaac ccagggttta cttcgtaaaa acaggagaaa ctatgactta
     2521 tgaggtagag gcccgggatg ccgaagaaaa aaaagtaccg gtaggaaaag tctacgggga
     2581 agtgtaccga ttagaatggg agaaggaaac ggagaagtgg agagaaatcc cttctcaatt
     2641 tttagatatt tctcttcctg aaggcacggg agagatgaaa ataactttag accgagccgg
     2701 ttactatcgc ctggaggttt atacccggga taggtcaggt aataaaatac tggcttcttc
     2761 ttacttctgg gtgacaggga aagatttaca ctatccttct cgtgaactcg aagtgcgctg
     2821 ggatagagag cgctatcgag tgggtgagga agccagggtt ttaatcgttc ctcctggaga
     2881 agggactttc acctttctct tttctataga gggctctgag cttcaagaag cggaagtaaa
     2941 gcaaatatct gcaccggagg aaatcgttat tccggtttta ccttcttacg tgatgggagt
     3001 ttatgtttct ttttcggggt tcagcaacgg gaaactatat tctgaatctc tgtccctccc
     3061 tcttgagcga gagaaagaat tgcaaataaa aataaccccg gaccgggaga gatatcaacc
     3121 ggaagaaaaa ggtaaaatct ctattcaggt gagcgatgct caaggaaaac cggtaaagtg
     3181 tgaattgtcg tttgctatag ttgaccaggc tatttttgac ctgagttcgt ggtggagaga
     3241 aaacgtatac gactatttct actctcaatt ttataactta gtttatggct cttactccat
     3301 ttattcttct ttttacggga tgggtatacc tttccaggat atgaaagccc agcttaaggg
     3361 gatgggagga gcggagggag gattattgtt gcggaagtat tttcctgaca ctcttttgtg
     3421 gaggccgacc ctgtcaaccg atgaagaagg aaaggcagaa gtagattttg ttgctcctca
     3481 ttctctcact cgctggcggg tagaagtaaa agcccaccag ggagaatttt ttggagaagc
     3541 gcaagagtac tttaccactt atcaaccttt tgctttagga gtggaacttc cttcctttct
     3601 ggtagaagga gacatcctga aaagtaaagc tactctctgg aatgaagtgg caccccagga
     3661 agtggtaata gagggggaaa gctccccgga aatttccctc gagtggaaga gggagcctct
     3721 aaaagtatct cttctggaag aggcagtgtt tcctctcgag gtaaaagctc aatatccagg
     3781 gaaagcttat cttcggcttt ttgctcgagg ggaatatgca caagacgcca tagagcttcc
     3841 tctggagata aaagaaagag gagtagaagt agaagagatt ttttattcct ttttagaaga
     3901 aaagaaaacc tcctttgagt tttcctgtga ttcttcttcg tctgtggagc ttgctctatt
     3961 ttctgatcct cgcagcatac ttttagagga gatagaatat atgctttcct atccttaccg
     4021 atgtagcgag caggtagcca gccgcttaat tactttttcc tctctacctt tggggaagat
     4081 ggaagataaa attctcgagg tcacagaagc gataagagag gatatttatt ccctctataa
     4141 cctgcagagc tatgatggag gatggggctt ctggggagga gaaggagatt tattccacac
     4201 tgcctatacc cttttggctt tgcacaaagt aaaagagagg ggttttctgg tcaatgctga
     4261 agtaatagag cggggattaa gctttttaac tcaagaaata tcaagaagcg aagctccgtc
     4321 ttcagattct actttttcac ccccgagtga gtttgacaga gctctatcct ggtatgctct
     4381 ttctctatgg aagaaagact ttgttccttc tcccctgtcc cctctggagg aaatggacga
     4441 taaaacttta gctcttcgag gtttggtggc ccgggaagaa gacaaagaga aagtggaaaa
     4501 agagttagag aaaagggctc atgttctggg tgatacggta ttctggggga caattaatcc
     4561 ccaaaaacct tgggaagatg accgttttcc ggctacagct tttgctgcct tgtttttggc
     4621 agagagagga aaccccctgg gagagaaagc ttacctgtgg ctgtggcgga gcaaaaaagg
     4681 aggaaattta tttactactc ttaaccgtgc ttactttttt ctcgttagct ctcacttttt
     4741 aaaagaagaa agtggtgttc ccacttttcg ggtgttttgt aacggagcat tagttaaaga
     4801 agggaaagaa gaagagaaaa tagctattcc ccggcaattt ttgaaggaag gctccaatca
     4861 tctggaggta gaaggagata acctgttcat gaccgaactc aaagtaaaaa gttggaagaa
     4921 ggaagcccct tcctcccact ttttctcggt aaagaaaaat tattatcttt tagtacccac
     4981 aaaaggagaa aaagttttct tctctcctca gaaagtagat gctttccatt ctcaggagga
     5041 agtagtttgt gagatagaaa ttcagagccc cgattactta gaatatctga taatagaaga
     5101 cgggatgcct gctggttgtg agctaatcag gaaagactat accttttctc tccctgagga
     5161 agaaggagca cattatcccg gagtgataaa aactcttttt gagggacagc ccactctttt
     5221 tgtgtatgaa ctttttcccg ggaaaaatgt gattcgctac tacttaagaa tccgggacca
     5281 gggtaatttc tatgtgaagg caccccttct ttatctcatg tatttccctg aagttagaga
     5341 ttatggagaa gaaaagaatc tggtggtgga ataactcctg tatccggagt ttttttctgg
     5401 gagtggtgat tatcctcttt ttatctcaaa gaggaggggc tcttcctgcc ttcgtgatag
     5461 agggtgggga gagcttccct tttttacctc cgatagtaga atcgagctat aaagaagctc
     5521 aactctgttt tcaaagagtg accggctact ccccttgttc ctcttttcgc atcaaagttt
     5581 tatctccaga aatttctaac actttacctt cctgggtggc agcgttttcg cgaggtagta
     5641 ccatttatct tcgctccccg cttttacttt tccagcgagg aattctttct tccaccctgg
     5701 ctcatgaatt tttccatgtt tttgtggaaa gctttggctg gcagcttcct tcctggtttg
     5761 aagagggtat ggcttctttc tttttcccca cccccgggga aatattcccc gaaggagcgg
     5821 gcgagtttta cgaggggtgt caggaatctt tccggatttt taaggaaaaa ataggagagg
     5881 aaaaccttcc ctcttttttc tctactgttc aggaaatggg ttttgaagaa gcttttttta
     5941 tctggacggg agaaaaagaa gaagattttg gtaaacatat tgatggagaa tgatgttatt
     6001 cttaaattta aaaatgtagg aaaaaaaata agggacagag taatcctgga aggagtatct
     6061 tttgaggtca gagaaggaga aatattcgga cttctgggag gaaacggagc aggaaagagc
     6121 actttgatac gccttctttt tggtctggtt ctgccttctg cggggtattt ttatgtggga
     6181 gattatcgct gccctcccca taatcccgag gctaaagcac tgatgggagg atgtctggat
     6241 aacacttctt tttacgggaa tttaaccggg atagaaaata tcactctttt tgcctctctg
     6301 tcttcctgct acccccgccg gggggagata gagtattttg cttctcttct ggggtttgag
     6361 ccatccctgc tttataagaa agtaagtggt tattctcaag gagagaagaa aaagctttct
     6421 cttctccatt ctctttttcc tcttccccga attttagttt gggatgaacc atgggtgggg
     6481 cttgaccccc ggggagcgaa agaagtccag gagctcattt tagctttaaa tgccgaggag
     6541 agggtaactt ttttcctttc ttctcctgtt tctcaggagg tggaaaagat agctcaaaga
     6601 gcagccattt tgcaccaggg taaaataaag tggaatgatt atttaactcc taatgtttct
     6661 ctggaagaaa tttactggga gataatagaa agaaaatgaa agctataaaa gcagagatca
     6721 aaaaagcaat tcataaccct cgcttctacg gagggatttt gatttcctgg gctctggcag
     6781 gtttagtagt agctcaagcc tggcgtgacc ccctctcttt ttatttggga aaaatcactc
     6841 tttccttttc tcttttatat cctctggcac tttttggtcc ggtgggggga atctggggag
     6901 gaacagaaaa tattgccgaa gagagaatgg aaggaactct atcaactact cttctccgcc
     6961 ccctctctcg ctgggagtgg tattggggga aagcaggatt cactcttttt tataacctgc
     7021 tcttggtttt cactcctctt ttagggatgg cattgctgga aaaaattatg tggggaggag
     7081 ggttttcttt ccgcctttta ggaggagggg tgatggttgc gctctacatt agctcttttt
     7141 ctgctttatc ttatcttttc tcttctcttt tctatcgtcc ctggggagga cctcttctgg
     7201 ctctgggaat aggagctttt tcctggatag tagagcgcct gggaggagga gaaaatgtcc
     7261 agatttatct tatcacccat cacctttctt ctttcaacat ctttttctta ccccggattg
     7321 attggcatca agcccttagc tcttttttaa ttctgggaag ctacaccgga ggagggcttc
     7381 tcataggagc gctcattttt atgagacagg aagaaaaata aggtttataa gggagcaaca
     7441 ccccccttat aaacccccga agatacaaaa catctaaaaa aagacataaa taaaaaacta
     7501 ccgggagaaa aagcatatct cccggtatag gggatccttt ttccccttat actggggaag
     7561 acgctcttcc ttcctcagta gttttttacc gagggaggct ctttctccct tggttgcatt
     7621 tattttttct gctttttcaa ttcctttctt ttttgcttac gccgtaggct gtctgttttt
     7681 tatctttatg ttttctgggg gttataaggg ggatctttct tcccccttat agttcattca
     7741 ctcttcaaaa gctcgagcaa ctttctctaa aagctcgggg atggggagct cttgaggaca
     7801 taaatcttca caatctccac acccgagaca tgaagaggct ctttcttcct caggaagagc
     7861 taaatatttc cgacgagcgt cctcaaaagc aatcttttga aaggcatcat tataaagctc
     7921 aaaattgcgg gagatattta ccccgttagg gcaaggaaga caatatttac acgcattaca
     7981 gttgatagaa gggagtcttt caaaagcctc tcgagctctc tcaattatct ttaactcctc
     8041 cggggataat aaacctactc ccgaagaaga agcgatttct atgttctcct tgacctgctg
     8101 gaggttgctc atcccgctca gaaccaccgc tacttctttt tgattccaca gccacaggag
     8161 ggcccaggct actggacttc ttttaacggg agcctcgtca aaaatttttt gcacttcctc
     8221 tggaggattg gctaatcttc ctccccgcaa aggttccatt attaccaccg ctatcccctt
     8281 ttgagctgca tattgaagac caaattgccc ggcttggtgg tcaatgtcga gataattgta
     8341 ttgaatctgg cagaaagtcc agggataagc atcgattatt tttttaaaag tttcccggtc
     8401 gtcgtgaaaa gaaaaaccaa tatgaccaat ctttcctttt tgaatggcct tttctgccca
     8461 gttcagcgct tccaaacgct caagtttagc ccaccggtct ttattcaggt catggaaaag
     8521 ataaaaatcg atgtgctcgg tgtctaactt tctaagttgc tcatccaagt agcggtccat
     8581 atctggagca gcattaacta accaggtagg aagcttggtg gctactttta ctttttgtcg
     8641 atatccacct tttagagctt ttccgaccaa tatttcactc ttcccgccat ggtagccgta
     8701 ggcggtatct aaataattta ctccgtggtc aatggcataa tgaatcattt ctaaagcttg
     8761 agcctcatct atatcggtat aatccgcgct ttttgagggt aaccgcatag ctccaaatcc
     8821 cagagctgaa acctggaaat ccaatttccc gaaagggcga tactgcatta attctcctcc
     8881 tttatttttg gccagtgtta tttatgggtc tttattatat ttttaccata tcggtcaaga
     8941 gagatattgg atggtggtaa aaatggtaaa atattgtgta attgtttaaa aattttttat
     9001 atcacctaat taattataac tatggtcaga gaacaaatga ttgaatattt aaccgacctc
     9061 gaaaaaagag atttattaga tgtaagagag ctgcttattg agtatgctga ttggttaaat
     9121 ttagacctgt cgtttcaggg attttctgaa gagattgaga ctcttcccgg gagatattct
     9181 cctccagaag gagctttgat tttagctcga gtcgatggag tagctgcggg atgcgtagct
     9241 ttgagaaagc tatcttctct cgtatgcgaa atgaaacggc tgtttgtccg cccctcatat
     9301 cggggcatgg gcattggtaa acaactggta gcgaggatta ttgaagaggc aataaagaga
     9361 cactatcagt ttatgaggct cgacactctg cctctaatga aagatgctcg aaagatttat
     9421 caggatatgg gattttatcc catatctcct tactatgata atcccattgt gggggccgag
     9481 ttcatggagc taaatcttga gaactggtca aagaagggaa cccgcttata gttctccccg
     9541 gaaaggcgag gacgaagtta agggagagaa aaaagcggca ctccctccaa actgtcattg
     9601 taagccactg aggaacaaag agcaccttcc tcagtgcttt ttgttctttt atattttcaa
     9661 tcttttgtct ttttaatttc acgtcgtaga ccgtccgttt tccctccctc agttattttt
     9721 gtctttagtc ttttattttt tacctttctt atgtttttca tcttacgccg tgggctgtct
     9781 gtggcgatcc cgaattttta gcgtaatgtg actagctttt aagtcttgag cgatttagca
     9841 gattttgcgg gatactataa aactttccat tcttaaaaac acctcttatg gacaacatat
     9901 tggggagttg gagagggtaa aataaaaaag ggcaatgtgg cttaaaaaaa gccttcttga
     9961 acactggtgg gccgtgcagg aatcgaacct gcagcctact gattaagagt cagttgctct
    10021 gccaattgag ctaacggccc atctagcggt tatcaatata ctatggatag ataaagatgt
    10081 caacacaccc tcactgcaaa tgttctggtg ttaactactt taatattgct gttttcgaga
    10141 actgtttgtt caaga
//
LOCUS       DAOPSS010000054         9793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_5805_length_9793_cov_8.462005, whole genome shotgun sequence.
ACCESSION   DAOPSS010000054 DAOPSS010000000
VERSION     DAOPSS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9793
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5805_length_9793_cov_8.462005"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..670)
                     /locus_tag="PKN31_04600"
     CDS             complement(236..670)
                     /locus_tag="PKN31_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOA99238.1"
                     /translation="MKIPTRYFGEIEVEEGKFILFPWGLPGFENLRQFLLLEEEGFFW
                     LQSVEERDVIFAVCDPFLYFPDYEVDIPSSECQALGIISEEEVIVLAIMNIQSPQDIK
                     VNLLAPVIINCRLKMGKQIVLEDERYSLHHPLFPEKASSSAG"
     gene            complement(684..1865)
                     /gene="flgL"
                     /locus_tag="PKN31_04605"
     CDS             complement(684..1865)
                     /gene="flgL"
                     /locus_tag="PKN31_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOA99239.1"
                     /translation="MRVTQKILTDRSMQNLNNILSRVLRTQDILSSGKEIRRPSDDPV
                     RFNQVLSLRTSIEKLDQYTSNVEDGINWLNLVDESLSSVSGVLEKVRTLVLQGANGTL
                     TAEDREMIATEIEAYLEELIGTANTSYAGRHLFSGTQTLTVPFSREGDIITYYGDNNS
                     IILEIENNTTLAIGFPGDDVFFRGFEASSGDMTSVDFSLHHNNSFTINGKTVNIDSAT
                     ITSLSDLVNLINNDPNLKSEVYALSDGDRLILRSRTASPLSLSGAPLEAWGMIDGGGD
                     ILNSREAEGVFKVVEDIIKDLRNNEVSKLSGEDLGNLDEALDNLLAVRSQVGARTLRL
                     ENSRNRFEEFSVNFKKLLSLNEDIDVAEVVMQLNEHQNVYQMALAATARVIQPTLLDF
                     L"
     gene            complement(1878..3269)
                     /gene="flgK"
                     /locus_tag="PKN31_04610"
     CDS             complement(1878..3269)
                     /gene="flgK"
                     /locus_tag="PKN31_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOA99240.1"
                     /translation="MSNAFFGLEIAKRALQAQQVAMDVTGHNIANSNTVGYTRQSPKF
                     SQETVSSGGLFVLPHLRNLGAGVSVNEVQRLRDKFIDLQIRQESRTASYWKTIDEGLE
                     QIEVIFGEPQEGALSEIFDRFWNTWQELAKSPESQAARALVAETANLLSTAFNHTHNQ
                     FVVQQQQLNEKIAIKVDEVNNYVQQIYDLNQQIMKLSLGGGNANDYKDQLDLLVDKLS
                     NVLDFQVSENSNGTYSLILQGRILVSDKEKRFLEVEANSTTGFWEVKFQGEEQINLDY
                     QNGELKALFYLRDEVVEEYKKYLNDLAGGIINAVNTAHKQGYTLEDPPVKGGDFFVGQ
                     DAATIALNPSIDVDPGKIAASLTGAPGDGENALAVARLRDQAIVDGSTPDDYYRGVVS
                     RLGVEKAESSRLLTNQNLLIDQLDMRKEAVSGVSLDEEMTNLIKYQYAYQAASLLTRT
                     VDEMLDTLVNRIK"
     gene            complement(3291..3800)
                     /locus_tag="PKN31_04615"
     CDS             complement(3291..3800)
                     /locus_tag="PKN31_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOA99241.1"
                     /translation="MIALNDFLSHLLTILEKELRLQTELWQVAEEKRDLLLKNQLEEL
                     VFLLEKEMNLVIQVRDTEKQLTDVWENMVERCNLEPGELNLGRIIELSPPDLALKFGE
                     VQRELKRVVRALREVNSQNAILIDDTLRYIDVVFSLLMGEGENSFYSPRGKEKPAKES
                     KGILINGVI"
     gene            complement(3797..4126)
                     /gene="flgM"
                     /locus_tag="PKN31_04620"
     CDS             complement(3797..4126)
                     /gene="flgM"
                     /locus_tag="PKN31_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOA99242.1"
                     /translation="MIEVKISSNQMESILRIYVNKAKEGEKTVFSPRKEEEGEGDKVF
                     LSRTGEKMGKLQQQYKKIPDIRADLVRELKVKIEKGEYEVRGREVAEKMFAREIADFL
                     INGGKDI"
     gene            complement(4176..4619)
                     /locus_tag="PKN31_04625"
     CDS             complement(4176..4619)
                     /locus_tag="PKN31_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99243.1"
                     /translation="MALAKCKRCKNLFNKIVDKDICPACMEKEEEEFQKVKDFLKRYP
                     EKKLEEISEETGVDKKIILDFIREGRLQLAEIQGEGEFALLTCERCGKSIVTGRFCED
                     CRKKLAALLSSDGDKPSSRSLDEDKLSSRSPNFYFKDAIKKKKEG"
     gene            complement(4606..4902)
                     /locus_tag="PKN31_04630"
     CDS             complement(4606..4902)
                     /locus_tag="PKN31_04630"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOA99244.1"
                     /translation="MRIENKELAISLKPERQSPQVVKKACQDFEAFFLGFIFKKGFQP
                     LFAPSSSPFFSQEETWFREMWVDEIAKKVGENGGWGLSDMLYRKLVEENKSGFS"
     gene            complement(5013..6134)
                     /locus_tag="PKN31_04635"
     CDS             complement(5013..6134)
                     /locus_tag="PKN31_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOA99245.1"
                     /translation="MKIKKSLALFCLVLTVFLAVSPAGGETVRIKDITEVEGVRGNQL
                     VGYGLVVGLAGSGDSRNSLFTNQALANMLEKMGIAVDSQAVRARNVASVIVTAELPPF
                     VQSGEEIDVTVSSLGDAKTLQGGVLLLTPLKAVDGKVYAVAQGPLSIGGFNVGGGGGN
                     VVQQNHPTVGMIPGGGIVERTVETNFVDEVRGVFTLLLREPDFVTASRIARAINEELG
                     GGKARVLDANRVEVSIPEHYQNQVSRLIALVGEIPVEPDVPARVVINERTGTVVIGGN
                     VRILPVAVSHGNLTVTVRTEYQVSQPPPFSPGETVVVPQREVRVEEEPARLFKVESGN
                     TIDDLVKALNALGVTPRDLIAILQAIKKAGALQGELIIE"
     gene            complement(6147..6752)
                     /locus_tag="PKN31_04640"
     CDS             complement(6147..6752)
                     /locus_tag="PKN31_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOA99246.1"
                     /translation="MKRVTAVFFMVVFVSLFFFSEEAKGESLWQDEGWFSDPYSDYRA
                     SRVGDTVTVIINENTKGKNSASSDSNKQTSIGLKAGQGIFDFVPPATLESGNSRGGSR
                     DYGRSISLSGTITCQVTEVLEGGNLRIAGSKEIYLNKERETLSIEGVVNPRYIGADNS
                     VYSTQVVDAVIKLDGTLKPEKKSGLAGLLEGVFGSIMDILF"
     gene            complement(6749..7732)
                     /gene="flgA"
                     /locus_tag="PKN31_04645"
     CDS             complement(6749..7732)
                     /gene="flgA"
                     /locus_tag="PKN31_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOA99247.1"
                     /translation="MKRKVVGCYFFWFLVILCLIGGGEVGALTLEVNLKEKVEVSAEN
                     IALGEIAEISYPEREWQRHLENLDLGYLPPPGGEREIKPEEIYERVVAQKVPQLDYIY
                     FSGAPVCKVITKGELIARGELEEKIQEQLLDIFPFARKIEVNLSSPGEVIIPPGGEVN
                     IVFSSSSSLKPWGTGNGTLEILREGKVEKTLPLRFEIQVYREVVRAKEKLSRGEVISE
                     DQVCSALEVLDYRNEKAFSSVEEVEGKQTKREISAGAILTPSFIQEETLIKRGDLVTM
                     VAKHGGIVVTGTGKARGEGSLGDVIVVENPDSKKRFEGEIIGERMVEVVVK"
     gene            complement(7887..8675)
                     /gene="flgG"
                     /locus_tag="PKN31_04650"
     CDS             complement(7887..8675)
                     /gene="flgG"
                     /locus_tag="PKN31_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOA99248.1"
                     /translation="MIRALWTAATGMQAKQMDLDVIANNLANVNTTGFKKSRVDFQDL
                     MYQVVRVKGTPTSPDTQVPSGIEIGLGVRPGAVQKMFSQGDIYETSNPLDVVIEGDGF
                     FQILTPDGTIAYSRDGSFKLDAQGRMVTSDGFPLEPPITIPEGAESVTVAQDGTVTVT
                     MPGEIEPQEVGVIELARFINPAGLLSIGRNLYLATASSGEPQIGTPGLEGFGTLAQGF
                     LETSNVQIVEEMVRMISAQRAYEINSKAITTADEMMSIANNLKR"
     gene            complement(8722..9471)
                     /gene="flgF"
                     /locus_tag="PKN31_04655"
     CDS             complement(8722..9471)
                     /gene="flgF"
                     /locus_tag="PKN31_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOA99249.1"
                     /translation="MIRGIYTATSALNLQELKQEVWANNIANLDTPGFKKELTWAEAA
                     REANLFRFLGSQNPYSLGRMTAGVQPGTEKLTDFSPGRLEETGNYLDLAIEGEGFFVV
                     STPSGEAYTRAGNFQLDEEGRLVTVSGYPVVGEGGEIILPAGGSVSVDEEGNISVDGE
                     EVDRLRIVDFLNRAVLRKSGENLFVAEGVIPQETTNFELRQGFLEKSNLDPVEAMVTM
                     MEALREYEIAQRILVSQDEALQKAVNSIAQL"
BASE COUNT         2775 a         2528 c         1619 g         2871 t
ORIGIN      
        1 ggtggaagaa agaataacct ccaccgccaa cggctctatc acttctgttc aggctattta
       61 tgttcctgct gataacctta ccgagctcga gacatcagaa tgagtgaaaa ttgagagaaa
      121 gtgacaaact tggggaaaaa gacaaaaact actttgggaa acaaccctca tgacccccct
      181 ctccacacca cacgagcagg caagactcat aaagacgccc ggaaaaaaag aaagcctatc
      241 ctgcagaaga gctagctttc tcaggaaaaa ggggatgatg cagactatat cgctcatctt
      301 ccaaaacaat ctgctttccc atctttaatc gacaatttat gattactgga gccaaaagat
      361 taactttgat atcttgaggc gattgaatgt tcataatagc cagaactata acttcttcct
      421 cgctaattat tcctaaagcc tggcattccg aagaaggaat atctacctca taatcaggaa
      481 aatagagaaa agggtcacaa acagcgaaaa ttacgtctct ttcttccaca ctctgcaacc
      541 agaaaaaacc ctcttcttct aaaagaagga actgccttaa attctcaaaa ccgggcaatc
      601 cccaggggaa aagaataaat ttgccctctt ccacttctat ctcaccaaaa taacgagtgg
      661 gtatcttcat ctctttctcc ttcttaaaga aagtccagaa gagtgggttg aatcacccga
      721 gcagtagcag ctaaagccat ttgatagaca ttctggtgct catttaattg catcactact
      781 tctgccacat caatgtcttc attcagagaa agtaattttt tgaaattaac agagaattcc
      841 tcaaaccgat ttcgagagtt ttccagacgc aaagtgcgcg ctcctacctg agagcgaaca
      901 gccagcaaat tatcgagagc ctcatccaga tttcccagat cctctccaga aagcttggat
      961 acctcattat ttcggaggtc ttttattata tcttccacca ccttaaatac tccctcagcc
     1021 tctcgagaat tcaagatatc cccaccgcca tcaatcatcc cccaagcctc aagaggagcc
     1081 cctgataaac tcagagggct agcagtgcga gagcggagaa taagacggtc tccatcggag
     1141 agggcataaa cctcagactt taagttgggg tcattattaa tgagatttac taaatctgat
     1201 aagctagtta tagtagcaga atctatgtta actgtttttc cgttgatggt aaaagagtta
     1261 ttatgatgta gactaaagtc aacagaggtc atgtcaccag atgaagcttc aaaaccccgg
     1321 aagaaaacat catctcctgg aaacccgata gccaaagtgg tattattttc tatttctaat
     1381 ataatggaat tattatctcc atagtaggta attatgtctc cttcccggga aaaaggaaca
     1441 gtaagagttt gcgtaccaga gaaaagatgc cttcctgcat aagaagtatt agctgttcct
     1501 atcaattctt ctaaatatgc ctctatttca gtcgctatca tttctcggtc ttcagcagtt
     1561 aaagtaccat tagctccctg taaaaccaga gtcctgactt tttctaatac cccggaaacg
     1621 gagctcaagc tttcatctac caggttcaac cagttaattc catcttctac gttagaagtg
     1681 tactgatcca gtttttcaat agaggtacgc aagctcaaca cctggttaaa gcgcaccggg
     1741 tcatcagaag gacggcggat ctctttccct gaggagagaa tgtcctgagt gcgaagcacc
     1801 cggcttaaaa tattattcag attctgcata gagcgatcgg taagaatttt ctgggttact
     1861 cgcattttca ctcctcctta ttttatcctg ttcaccaggg tgtcaagcat ctcgtccacc
     1921 gttctagtca aaagagatgc agcctgatag gcatactgat attttattaa attggtcatc
     1981 tcctcatcta aagaaactcc tgaaaccgct tctttacgca tatctaactg atctatcagc
     2041 aaattctggt tagtaagtag acgactggat tccgcctttt ctactcccaa tcgagaaact
     2101 actcctcgat agtagtcatc tggagtagag ccatctacta tagcttgatc tcgcaagcga
     2161 gcaacagcca aagcattttc cccatctcca ggagcaccgg tcaaagaggc agcaatttta
     2221 ccggggtcaa cgtcaatgga agggttaaga gcaatggtgg cagcatcttg accaacgaaa
     2281 aaatctcctc cttttaccgg tggatcttcc agagtataac cctgcttgtg tgccgtattt
     2341 accgcattaa taattccccc agccaggtca ttcagatatt tcttatattc ttctactact
     2401 tcatcgcgga gataaaaaag agctttcagt tctccatttt gataatctaa attgatttgt
     2461 tcttctcctt ggaatttaac ttcccaaaaa ccggtggtag aattagcttc tacttctaaa
     2521 aatctctttt ccttatcact cactaatatt ctaccctgga gaatcaaact gtaagttcca
     2581 ttggaatttt cactcacttg gaagtctaaa acattagaaa gtttatccac cagaaggtct
     2641 aactggtctt tgtaatcgtt ggcattccct cctcctaagc tcagcttcat aatttgctgg
     2701 tttaaatcat aaatctgctg aacatagttg ttcacttcgt ccactttgat ggctattttt
     2761 tcattgagtt gctgttgttg aacaacaaat tgattgtgag tgtggttaaa agctgtactc
     2821 aacaaattag cagtttctgc taccaaagct cgagcagctt gcgattcagg agatttggcc
     2881 agctcttgcc aggtattcca aaagcggtca aaaatctccg acagagctcc ttcttgcggt
     2941 tctccaaaaa tcacctcaat ctgctccaag ccttcatcta tagttttcca ataagaagca
     3001 gtgcgagatt cctgtcgaat ctgtaagtct ataaacttat ctcgcagacg ctgaacctca
     3061 ttcactgaaa ctccagcacc taaattgcgt aaatgaggaa ggacaaaaag accccctgaa
     3121 gaaacagttt cctgagaaaa tttgggtgat tggcgagtat agcctaccgt gttagaattg
     3181 gcaatattgt gtccggtgac atccatagcc acttgctggg cttgtagagc tctcttagct
     3241 atttccagtc caaaaaaagc attgctcatt ttttatcccc ttctacttta ttatattact
     3301 ccgttgatta gaattccttt actttccttc gctggttttt cttttcctcg cggagaataa
     3361 aaactgttct ccccttctcc catcaaaaga gaaaaaacta catcgatata gcgcaaggta
     3421 tcatcaatca atatggcatt ctgagaatta acttctcgta atgctcttac cactcttttc
     3481 aattctctct gaacctcacc aaatttcagg gccagatcag gaggagaaag ctcgattatc
     3541 cgacccaagt tcagctctcc aggctctaag ttacatctct ccaccatatt ttcccagaca
     3601 tcggttaact gcttttctgt gtcccgtacc tggataacca aattcatttc cttctccaga
     3661 agaaaaacca gctcttccag ctggtttttc aaaagtaagt ctctcttctc ttcggctact
     3721 tgccagagct cagtctgcag acgcaattcc ttttccaaaa tcgtcaaaag atgagagaga
     3781 aaatcattca aggctatcaa atatctttcc ccccattaat taaaaaatca gcaatttccc
     3841 gggcaaacat cttttctgcg acttccctac cccgaacttc gtattctccc ttttctatct
     3901 tgacttttaa ctctcgaact aaatcagctc ttatgtcggg aatcttttta tactgttgct
     3961 gcagttttcc cattttctca ccagtgcgag aaagaaaaac tttgtctccc tctccctctt
     4021 cctctttcct aggagaaaaa acagtttttt caccctcttt agccttattt acataaattc
     4081 ttaaaatgct ttccatctgg ttagaagata ttttcacttc tatcacttcc tttaaaccta
     4141 ctcactatat tatatcggac gaattcctga tttctttagc cttctttttt cttttttatg
     4201 gcatctttaa aataaaagtt gggagagcgg gaagatagct tatcttcatc gagagagcgg
     4261 gaagatggtt tgtctccatc ggaagaaaga agggcagcaa gtttttttct gcaatcttca
     4321 caaaaccttc ctgtaacaat ggatttacca catcgctcac aggtaagaag agcaaactcc
     4381 ccttctccct gtatttcagc cagttgcagc cttccctccc gaataaaatc taaaattatt
     4441 ttcttatcta ctccggtttc ctcacttatc tcttctaact ttttttccgg ataacgtttc
     4501 aagaaatcct tgactttctg aaattcttcc tcttcttttt ccatacaggc aggacaaata
     4561 tctttatcta ctatcttatt aaataaattt ttgcaccttt tacatttagc taaagccact
     4621 cttattttcc tccactaatt ttcgataaag catgtcagac aggccccagc ccccattttc
     4681 cccaactttt ttagcaattt catccaccca catctcccga aaccaggttt cctcctgact
     4741 aaaaaaagga gaggaagaag gagcaaaaag gggttgaaag ccttttttaa aaataaaacc
     4801 aagaaagaaa gcttcaaaat cctgacaggc ttttttcact acctgaggag actgtctttc
     4861 gggtttcaga gaaatagcta actctttatt ttctatacgc atatcgccac cctctttatc
     4921 ctttagaaat atatcataat taatctatga tgccaacacc aaaaagcaaa aactggtgaa
     4981 tcagctttca taggaaaata ccataaaaaa acttattcta tgattaactc tccctgcaaa
     5041 gcaccggcct ttttgatagc ttgcaaaatg gcaatcaggt ctcgaggagt aactcctaaa
     5101 gcattgagag ctttgactaa gtcatctatg gtatttcctg attccacttt aaatagacgg
     5161 gcaggctctt cctccactct gacttcccgc tgaggaacca ctacagtttc tcccggagaa
     5221 aaaggaggag gctgggaaac ttgatattca gtcctcacgg tcacagtcaa attgccatga
     5281 gaaacagcta ccggtaatat ccgcacattc cccccgatta ccacagttcc cgtcctctca
     5341 tttatcacca cccgggcagg aacatcaggt tctaccggga tctctcccac cagagctatc
     5401 aatcgagata cctgattttg gtaatgctcg gggatactaa cttccacccg attggcatcc
     5461 agcacccgag ccttccctcc tcctaactcc tcattaatag ctcgagcaat acgagaagcg
     5521 gtcacaaaat ctggctcccg taatagaaga gtgaaaactc ctctcacctc atcgacaaaa
     5581 ttagtttcta ccgtcctctc cactattccc cctccgggaa tcatgcccac tgtgggatga
     5641 ttttgctgca ccacattccc tccccctcct cccacattga atcctccaat agaaagagga
     5701 ccttgagcta cagcatacac cttcccatct actgctttta agggagtaag cagtaacact
     5761 cctccttgca aggttttagc gtctcccagg gaagaaacag taacatctat ttcttctcct
     5821 gactgaacaa aagggggaag ctcggcggta actattaccg aagccacgtt ccgagctcgc
     5881 actgcctgag aatctacggc tatacccatt ttctccagca tattagctaa agcctgattg
     5941 gtaaaaaggc tattcctgct atctccactt cctgccagcc ccaccaccaa tccatatcct
     6001 accaattgat tacctcttac tccttctacc tcagtaatat cttttatcct cactgtttct
     6061 cctccggcgg gagaaacagc taaaaaaact gttagaacca aacaaaacaa agctaaactt
     6121 ttttttattt tcacctctac cacctgttaa aaaagaatat ccataatcga gccaaaaaca
     6181 ccctccaaaa gccctgccaa accgcttttc ttttctggct taagagttcc atccagtttt
     6241 atcactgcat ccactacctg agtagagtaa acactgttat ctgctcctat gtaccggggg
     6301 tttaccactc cctcaatact caaagtctcc ctttctttat ttaaataaat ctccttgctt
     6361 ccagcaatcc gcaaatttcc cccttccaac acttctgtga cctgacaggt aatggtgcca
     6421 gaaaggctaa tactacgccc gtaatcccta cttcctcctc gagaattacc gctttctaat
     6481 gtcgccggag gaacaaaatc aaaaatgccc tgccccgctt taaggccaat ggaggtttgt
     6541 ttattactat cagaggaagc actatttttc cccttcgtat tttcatttat aatcaccgtt
     6601 acggtatccc ccacccggga ggctcgataa tcagaatagg ggtcagaaaa ccatccttca
     6661 tcctgccaca gagactctcc ttttgcctct tcagaaaaga agaaaagaga aacgaaaacc
     6721 accatgaaaa agacagccgt tactcttttc atttcaccac cacctccacc attctctccc
     6781 ctataatttc gccttcaaat cttttcttag aatcaggatt ttctaccaca attacatctc
     6841 ccagactacc ttctcctcgc gctttcccag tgccggtaac cacaatacct ccgtgttttg
     6901 ctaccatcgt aaccaggtct cctcgtttaa taagagtttc ttcctgaata aaagaaggag
     6961 ttaaaattgc cccggcagaa atttctcttt tcgtttgttt tccttccact tcttctacac
     7021 tggaaaaagc cttttcattg cggtaatcta aaacttcaag agcagaacaa acctgatctt
     7081 cgcttataac ctctccccgg gaaagctttt ccttagctcg caccacttcc cgataaacct
     7141 gaatctcaaa acggagagga agagtctttt ctactttccc ttcccggaga atttccagag
     7201 ttccatttcc tgtcccccag ggctttagag aggaagaaga agaaaaaact atattaacct
     7261 cgccaccggg gggaattatt acttccccgg gagaggaaag attcacttct atttttcggg
     7321 caaaagggaa aatatctaat agctgttcct gaattttttc ttctaattca cctctcgcta
     7381 ttaattcccc cttagttatt actttacaaa ccggagctcc ggaaaaatag atataatcta
     7441 actgaggcac cttttgagcc actacccgct cgtatatttc ttctggtttt atttctcgtt
     7501 ctcctcccgg gggaggcaaa taccccagat ctaaattctc taagtgcctc tgccactccc
     7561 gttccggata ggaaatctct gctatttctc caagagcaat attttccgct gaaacttcca
     7621 ctttttcttt tagattcact tccagagtaa gagctcccac ctctccccca ccaattaaac
     7681 aaagaataac caaaaaccag aaaaagtaac atccaacaac ttttcttttc attactcctt
     7741 atccttcttc cactgaggga tccttactcc ctcagtggac tttctattcc ctcttataat
     7801 gaggaaagtt tttctctcat tagtttttcg ctcaagtttt tgtttttagg ggttataggg
     7861 aaattagttt cctcctccag tcattatcac ctctttaaat tattagcaat gctcatcatc
     7921 tcatcagcag tggttatagc tttggagttt atctcataag ctcgctgggc acttatcatc
     7981 cttaccattt cctctactat ttgaacattg gaagtctcca aaaaaccttg agctaaagtt
     8041 ccaaaaccct ctaaaccagg agtaccaatt tgaggttctc ccgaagaagc agtagctaag
     8101 tataagttcc ttcctatact caaaaggcca gcggggttaa taaacctagc cagctctatc
     8161 actcccactt cctgaggctc tatctctcct ggcatcgtga ctgtcactgt tccatcctga
     8221 gcaacggtaa ccgactccgc tccctcggga atagtgatgg gaggctcaag gggaaaacca
     8281 tcactggtta ccattcttcc ttgagcatct aatttgaaag aaccatcccg gctataagct
     8341 atcgttccat cgggcgtcaa aatttggaaa aaaccatctc cttcaattac cacatctaag
     8401 ggattagaag tctcataaat gtcaccttga gaaaacattt tctgcactgc ccccggcctc
     8461 actcccaaac ctatttcaat tcccgaggga acttgagtat caggagaagt aggagtcccc
     8521 ttcactctca ccacttgata cattaaatct tgaaagtcaa ctctactttt tttgaaaccg
     8581 gtggtattca cattggctaa attattggca atgacatcca ggtccatttg tttagcctgc
     8641 attcctgtcg ccgccgtcca aagagctcta atcattaaaa tcctcccttc ccagggcctc
     8701 ccgcaggacc agccctaatt tttaaagttg ggcaatactg ttaaccgctt tttgcagagc
     8761 ctcatcctgg ctaaccaaga tacgctgagc aatttcatat tctcgcaagg cttccatcat
     8821 ggttaccatt gcctctaccg gatcaaggtt agacttttcc agaaaacctt gtcggagctc
     8881 aaaattagta gtttcctgag gaattactcc ctctgcaaca aataaatttt caccactttt
     8941 tctgagaacc gctcgattta gaaaatctac tattctaagc cgatccactt cttctccatc
     9001 cactgaaata ttcccctctt catccaccga aacactccct ccggcaggta aaataatctc
     9061 tccgccttca cccacgactg gatagccgga tacagtaacc agcctaccct cttcatccag
     9121 ctggaagttg cctgctcgag tataagcctc cccagaaggg gtagaaacta caaaaaatcc
     9181 ctctccctcg atagccaaat ccagatagtt accagtttct tccaacctcc ccggagaaaa
     9241 gtcggtaagc ttctccgtcc ccggctgcac tccagcagtc attcttccta aagaataggg
     9301 attctgagag cccaaaaacc gaaataaatt agcctcccga gcagcttcag cccaggttag
     9361 ctccttttta aaaccaggtg tatccagatt agcaatgtta ttagcccata cttcttgctt
     9421 gagctcctgt aagttaagag ccgatgtagc agtataaatt ccccgaatca attccctatc
     9481 acctcctctc tgggtttttc tactaccatc tctggataaa tttatctgcc aaatactctc
     9541 ttttttaaaa agaaactcta aaattgggta cccttcataa gagaccacaa aaaaagagct
     9601 ccttgcaaac aagctgctct gttttttcat tcccaaaatc agtaatcgag aatccagagg
     9661 tttttaccaa gagaagcttt ttctctctca gttagtcttt attctttagg tttattgttt
     9721 ttcaaaaggg ggttataagg ggggagctcc tccttatagt ttggaccatg acaaaatcat
     9781 tcagggggtg acg
//
LOCUS       DAOPSS010000055         9696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_5885_length_9696_cov_8.134322, whole genome shotgun sequence.
ACCESSION   DAOPSS010000055 DAOPSS010000000
VERSION     DAOPSS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9696
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5885_length_9696_cov_8.134322"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..564)
                     /locus_tag="PKN31_04660"
     CDS             complement(145..564)
                     /locus_tag="PKN31_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOA99250.1"
                     /translation="MIKAFDHVTITVSDLERSIDFYQRVLGFKVLGMLEQNGGDFKIV
                     YLDAGGATIELFHFKEKGRPLTEAKDLDIGIKHIGFKVDNVDEVVKKLKEAGVRFTLE
                     PFDATGGVRIAFFKDPDGILIELIQGELELLPYKESQ"
     gene            complement(894..2357)
                     /gene="aglA"
                     /locus_tag="PKN31_04665"
     CDS             complement(894..2357)
                     /gene="aglA"
                     /locus_tag="PKN31_04665"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032450 - maltose alpha-glucosidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase AglA"
                     /protein_id="HOA99251.1"
                     /translation="MAATKLSIIGAGSAAFSLRLVGDLCKTKELAGSLVSFMDIDENR
                     LNAVYVLAKKYAEELGVDLEFETTTSVEDAIRDSSFVVNTALVGGHSYFEKVRRISEK
                     YGYYRGIDSQEFNMVSDYYTISNFNQLKFMYDVAKMIEKLSPKAWLLQAANPVFELTN
                     LISRNVPINMVGICHGHHAVDHMIEVLGLDKNKVDWQVAGVNHGIWLTRFLYEGKDAY
                     PLIDKWFEEELASWKPTNPFDDQLSPVAKDMYEFYGKMPIGDTVRNGSWKYHYNLETK
                     KKWFGEPWGGVDSELGWKWYQERQAEVAITMQKVARYFQENPEAKLLSRETYDEIVSL
                     AKDEVKEEFTQEVFKILDPEKKSGEQHILLVNALLNNEKVDLVLNVLNKGALPGIPDD
                     VAVEIPVYADKDGIHPYQVEPDIPERIKKMYLMPRILRMEWALEAFLTGDRRVLEEFL
                     IRDPRTKSYDQVVKVIDEVLGLPENEEMRKHYSNSRK"
     gene            complement(2522..3373)
                     /gene="amrB"
                     /locus_tag="PKN31_04670"
     CDS             complement(2522..3373)
                     /gene="amrB"
                     /locus_tag="PKN31_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOA99252.1"
                     /translation="MVRKAAVAGYFYPGRERELRQMIKELFYSEAGPHGKEEELTKWK
                     EVSGVVSPHAGYIYSGAVACWSFYSLSLASPVDTIVIIGPNHRGAGKRVALSDADFWE
                     TPLGEVAVDLEAVEFLASHYPLFSPDSRAHQLEHSVEVQIPFLQYFLPYPFQIVPVTV
                     WDQTLEVALEISRAVQELSRKRKIAVVASSDFSHYEPAQVAEAKDSHAISCIVSLQTE
                     EFYRVIRRENISICGPAGIAALMEYHKRRSQEEGKLLFYSHSGKVSGDYQEVVGYASL
                     IFPVTAE"
     gene            complement(3376..4740)
                     /locus_tag="PKN31_04675"
     CDS             complement(3376..4740)
                     /locus_tag="PKN31_04675"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOA99253.1"
                     /translation="MKFSREAFLAFMFLLIIFSVAEGASWEVEKIIEEVGGELPFLDY
                     PVGILVDDQNQLVLVNDWGNNRFLSLTPDGQIQKALTGLNSPAGLGKDEQRNRIYIVE
                     QKANQVRVINSDDFSTSGFLKAKTPLQEPRGIWIDEEGKIYVADTGKSRIVVFSPEGE
                     ELFTFGKEGMGNDEFYYPRGVAVDGDGHIWVVDTQHHCIKVFDEKGNFLFRFGQGGEG
                     EEDFNQPRYLFIQGNWVFISDYRNHRIKVYNREGVLQEIMGEEGRGEGEFSFPEGLWI
                     DKDGKLWIADAGNNRLQVIDVNFLINPKLHLASLLEEGESQKFMQVMERLSPEEREDP
                     EVARLLLEFFRKRGDLDRAIYQAEDLFLNDSANRDYWKKTLGELYYEKGVHLREEGKL
                     KEAQEFFRKSWQSGNSHSLFLYLWTSFLLLGGSNLFILILAIILLFLIMVFSRLRKGH
                     YRGW"
     gene            complement(4727..5317)
                     /locus_tag="PKN31_04680"
     CDS             complement(4727..5317)
                     /locus_tag="PKN31_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOA99254.1"
                     /translation="MIYYICKYLAIFLLKVFFSFRVKGVNHIPRQGPCILVANHSSNL
                     DPIVLGCASSRRVHFVAKEELFRNPLVSFFLRRLGAFPLHRGEGDKEAVKRIFSLLKE
                     GKVVGLFPEGTRNQGELGTFQRGALKLLLRAEVPVVVAGIRGTYESLPRDKKIPRPFP
                     IQVTFSPPFEVKGWSVEEVEKKIREEMEVLLSDEVQ"
     gene            6160..6354
                     /locus_tag="PKN31_04685"
     CDS             6160..6354
                     /locus_tag="PKN31_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HOA99255.1"
                     /translation="MPNITVEGPPISDIEKKRKLVQTITQVATEVYGLPAQAIVVTIR
                     ENSPENVAIGGNLLSDQKKS"
     gene            complement(6447..7223)
                     /locus_tag="PKN31_04690"
     CDS             complement(6447..7223)
                     /locus_tag="PKN31_04690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOA99256.1"
                     /translation="MITRLSVALDVAKEVGKFVMDHENHIQEIKEKNSPFDVVTEVDK
                     EAQEMIIEGLSSHFAQDLFWGEESNYPLEDFSSTWVIDPIDGTSNYIHNLPLYGISIA
                     YLREGEPIIGVLDFPRLGETFWAEKGKGAFLNGERIRVSDIKEMHRTIVGTGFPHQSA
                     KWQIMEPVYAQIAAECQALRSFGSAALGVAYVACGRLEGYLQLGLSFYDVAAAVCLVR
                     EAGGEVRELGNKEWNVRSRSILATNSKIGMEKFLKDFSLS"
     gene            complement(7226..8410)
                     /locus_tag="PKN31_04695"
     CDS             complement(7226..8410)
                     /locus_tag="PKN31_04695"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOA99257.1"
                     /translation="MPGFSIGELQRWLNSGEHDSVPVFSYDEEEKIVEVRRIGVLSGG
                     GDCPGINAVIHAVVKRAVLRYGWEVVGVLDGFEGMVEGKWRKLEYNDVSGIMPLGGTI
                     LGTSNKADPFRYPVHEEGKLVFRDFSQKVIDNYHQMDIQALIAIGGDGTFNVSQKLAE
                     KGLNIVGVPKTIDNDLGGTDLTFGFDSAVAVVTDAVDRLHTTAQSHHRVMVVEVMGRY
                     AGWIALYGGIAGGGDIILIPEIPFDIDKVCEFILKRGAAGKRFSIVVVAEGARPKGGE
                     MVVQKIVEESHDQIRLGGIGYKVGQDIERKTGLETRTVVLGHLQRGGSPTAFDRILAT
                     RFGAKAVDLIAEGKYGYFPALQGEEIVPLPMEVAISNLKVVPLDSPLLDIARSLGTCM
                     GD"
     gene            complement(8422..9330)
                     /locus_tag="PKN31_04700"
     CDS             complement(8422..9330)
                     /locus_tag="PKN31_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA99258.1"
                     /translation="MRLGFMTACLPEMELEEIVKWAKEKGFGMLEVACWPRVFEKRRY
                     AGTQHIDVENFTEEKAQEIKELFQSNYLEISSLGYYPNNLDPDLESRCFYHEHLKKVI
                     QAAEKLEVKVVGTFVGRDPRMNVEESLEEFKKVFPDLVKFAEDHGVKLAIENCPMLYS
                     LDRWPGGTNLATTPEIWRRMFEMIPSDYFGLNLDPSHLIWQQIDYVKAVYDFKEKIFH
                     VHAKDTRIDWNRLDEVGIFGFGWYIDKVAGTGDIDWNSFINALYEVGYNYVVSIEHED
                     RSFEKDQESKLRGILLAKQLLEPYIV"
     gene            complement(9346..>9696)
                     /locus_tag="PKN31_04705"
     CDS             complement(9346..>9696)
                     /locus_tag="PKN31_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA99259.1"
                     /translation="KNRCSFEIDGSRSAIFWDSERPNELWIGRRDRANELLLKDPSLV
                     SEEARKIISFPGGHNEGFPDTSKQLFREIYQCILEGKRGDFSFPTFTDGLREVVLCEA
                     ILRSAKEGKWIRVN"
BASE COUNT         2824 a         2465 c         1611 g         2796 t
ORIGIN      
        1 gccagcttag tgataaagtt cgtaccttgc cagtgctcta ataaacaggt cttccaaccg
       61 gccgatagtt cgtttctgaa gcacccatct ggccacaagg caaattttat tatcttaaat
      121 agtagctgga tattataaat aagttcactg gctttctttg taggggagta gctctaactc
      181 tccctgaatc agttctatga gtataccatc agggtctttg aaaaatgcaa ttctcactcc
      241 ccctgtggca tcaaaaggct caagagtgaa cctgacacca gcttctttca atttctttac
      301 cacttcatcc acgttatcca ccttaaaacc aatatgctta atccctatat ccaggtcttt
      361 tgcctctgta agaggtctgc ccttttcctt aaaatgaaat aattctatgg tggctcctcc
      421 ggcatccaga tacactatct taaagtcacc gccattttgc tctaacatgc ccaacacttt
      481 gaaccccaga acacgctggt aaaaatctat agagcgctcc aggtcagaaa cggtgatggt
      541 aacatggtca aaagctttta tcatctatca tccttccttt ccatgggaaa ttttcttcca
      601 gccttccatt ttcccgggga ttttcctgac acagatagtt tatccccaaa aggaaacaaa
      661 gtctatccag agaaggatag tagagcagtg ttctttctca aatttatctt tcatatcctc
      721 aatagatgag ttctgattta attcagggcc ttctgcagat ctttcacaaa atgcacctta
      781 ccccaacggc tctctctttt ttatttttac gaggatatag agcataaaga ccacaggagt
      841 aacatccatg ccccgggaga gactttgcct cgcactttta aaaggaacga tggctatttt
      901 ctggaatttg aataatgttt cctcatttct tcattttcag ggagaccaag tacttcgtct
      961 attactttaa ccacctggtc ataagattta gttcggggat cccttatcag gaattcttct
     1021 aagactcttc tatctcctgt cagaaaagct tctaaagccc actccattct taaaattctt
     1081 ggcatcagat acatcttttt aattctttcc ggaatatctg gttccacctg gtaaggatgt
     1141 atcccgtctt tatctgcgta aaccggaatt tccaccgcaa catcgtctgg aatccctgga
     1201 agcgctcctt tgttaagcac attcaaaaca aggtcaactt tctcattgtt taataaagca
     1261 ttaactaaca atatatgctg ctctccactt ttcttttccg ggtctagaat tttgaatact
     1321 tcctgcgtaa attcctcttt aacttcatct tttgcaagag aaactatttc atcgtacgtt
     1381 tcccgggata gcaattttgc ttctggattt tcctggaagt atctcgccac tttctgcatg
     1441 gttatcgcaa cttctgcctg cctttcctgg taccacttcc agcctaattc tgaatccact
     1501 cctccccatg gttcaccaaa ccattttttc ttggtttcga ggttgtaatg gtatttccag
     1561 cttccgttcc ttaccgtatc cccaatgggc attttcccgt agaattcgta catatccttc
     1621 gccacaggag atagctggtc atcaaatgga tttgttggtt tccaacttgc aagctcctcc
     1681 tcaaaccatt tatcaatcag aggataggca tcctttcctt cgtacaaaaa tctggtcagc
     1741 catattccat ggtttactcc ggcaacctgc cagtccactt tatttttatc caatcccaga
     1801 acctctatca tgtggtccac agcatgatga ccgtgacata tacctaccat gtttatagga
     1861 acattcctgg atattaaatt agtcagttca aaaacagggt ttgctgcttg caaaagccag
     1921 gctttgggcg aaagtttctc tatcattttt gcaacatcat acataaattt tagttgattg
     1981 aaattactga ttgtataata atcagaaacc atattaaatt cctgagaatc tatccctcta
     2041 taataaccat atttctcaga aattcgcctg actttttcaa aataactatg tccgccaacc
     2101 agcgctgtat taacaacaaa agatgaatcc cttatcgcat cctccacact ggtagtggtt
     2161 tcaaattcca aatcaactcc cagctcttca gcatattttt tagctaatac atacactgcg
     2221 ttcaacctgt tctcatcaat atccataaag gatacaagac taccggctaa ctcctttgtt
     2281 ttacaaagat ctcctaccaa tctcagagaa aacgctgcac ttcctgcccc aataatgctt
     2341 aatttggtgg ctgccattat caccatacct ccttaaagtt gacttttagc taactttata
     2401 aattccggaa tccagaagca aatccagtaa aaacaattta aaagactctt cacttaaaat
     2461 gcacattgga cgataaaaag cttatagcaa attatagccc tccaggataa ataacctgct
     2521 tttattctgc agtaacagga aaaattagag aagcatatcc cactacttct tgatagtcac
     2581 cacttacctt tccactatgg gagtaaaaga gcagttttcc ttcctcctgt gaacgccttt
     2641 tatgatactc catcaaagca gcaatccctg ccgggccaca gatgcttata ttttccctcc
     2701 ttatcaccct atagaactcc tcagtctgca aagaaacaat acaggatata gcatggctat
     2761 cttttgcttc tgctacctgg gctggctcat aatgagaaaa atcactactg gccactaccg
     2821 ctattttacg cttccgagat agttcttgca cagctcgact tatttctaaa gctacttcca
     2881 aagtttgatc ccagacagta acgggtacaa tttgaaaggg ataaggaaga aaatattgca
     2941 aaaatggaat ctggacttcc acagaatgct ccagttgatg agcccgggaa tcgggagaaa
     3001 aaagaggata atgagaagcg aggaattcca ccgcttccag atctaccgct acttctccca
     3061 gaggtgtttc ccagaaatcg gcatcactta aagctactct tttacctgct cctcgatggt
     3121 tgggcccaat tataactatg gtatccaccg gagaagccag agaaagagag taaaacgacc
     3181 agcaagccac tgctccagaa tagatgtagc ctgcatgggg actgactact ccacttacct
     3241 ctttccattt tgttagctcc tcttcctttc catgaggacc tgcttcagag taaaaaagct
     3301 ctttaatcat ctggcggagc tccctctccc gaccgggata gaaatacccc gctaccgccg
     3361 cttttctaac catctctacc accctctata atgtcctttt ctcaaacgag aaaataccat
     3421 aatcaaaaac aagagaatga ttgccagaat gagaatgaaa agattggagc ctcccagaag
     3481 gaggaaggaa gtccagagat aaaggaaaag agaatgactg ttaccactct gccaggactt
     3541 acggaaaaac tcctgggctt cttttaattt tccttcttcc cgcagatgga cacctttctc
     3601 gtagtaaagc tctcccaaag ttttcttcca gtaatcccga tttgcactat cattcagaaa
     3661 taagtcctct gcttgataaa ttgccctatc taagtctcct ctttttcgga agaactccag
     3721 tagcagacga gctacctcgg ggtcttctct ttcttcggga gaaagtctct ccatcacttg
     3781 catgaatttc tgactttctc cttcttccag caaagaagct aaatgcaatt tagggttgat
     3841 taaaaagtta acatcaataa cctgcagacg attattgcca gcatcagcta tccataattt
     3901 gccatctttg tctatccaga gaccttcagg aaaggagaac tctccctcac cccgcccttc
     3961 ctctcccatt atctcctgga gaactccctc ccggttatat acttttatgc ggtgattgcg
     4021 ataatcagaa ataaataccc agtttccttg aatgaataaa tagcgaggtt gattgaagtc
     4081 ttcttcccct tctcctccct gaccaaaacg gaagaggaaa ttaccctttt cgtcaaacac
     4141 ttttatgcag tgatgttgag tatctaccac ccagatatga ccatctccat ctactgccac
     4201 ccctcgagga taataaaact catcattccc cataccttcc ttcccaaagg tgaaaagctc
     4261 ctccccttct ggactgaaaa ccactattcg agatttaccg gtatcagcca cgtatatttt
     4321 cccctcttca tctatccaga taccccgggg ctcttgtaga ggagttttag ctttcaaaaa
     4381 accagaggta gagaaatcat cactatttat aactctcact tgattggctt tttgctctac
     4441 aatgtagatt ctattccgtt gttcatcttt tcccaggcct gccggactat tcaatccggt
     4501 taaagctttc tgtatctgtc catccggggt taaggaaaga aatctgttat tcccccaatc
     4561 attaactaaa actaactgat tctggtcatc taccagaata cccactggat aatctaaaaa
     4621 aggcaactct ccccccactt cttcaattat cttttctact tcccaggaag ctccctctgc
     4681 cacagaaaaa ataatcaata gaaacataaa cgctaaaaaa gcttctctac tgaacttcat
     4741 cactcaataa tacctccatt tcctccctga tcttcttctc tacttcttcc acactccaac
     4801 ctttaacttc aaaaggagga gaaaaggtca cctggatggg gaaaggacgg ggtatcttct
     4861 tatctcgggg caggctttca taagtccccc ggataccggc taccaccact ggcacttcag
     4921 cgcgaagaag aagcttcagg gctcctctct ggaaagttcc cagttccccc tgattacgag
     4981 ttccttcggg gaaaagacct actacttttc cctctttaag aagagaaaaa atccttttca
     5041 ctgcttcctt atctccttct ccccggtgaa gaggaaaagc tcctaaacgc cgtaagaaaa
     5101 aggagactaa agggttacgg aaaagttcct ctttagctac aaaatgtact ctccgagaag
     5161 aggcacatcc cagcacaatg gggtcaagat tgctggaatg gttagccacc aaaatacaag
     5221 gcccctgcct gggaatatga tttacccctt tcactcgaaa agagaaaaaa accttcaaca
     5281 agaaaatggc taaatactta catatataat atatcatttc tcttcctatt ttactccctt
     5341 tcctctgtga ggttctactc caattttaga gaaaaattcg gggtatcaaa aggttctgca
     5401 tcaaaaaccg gatatactga atgctaaact gaattccacc aaacaagcta agacttgaat
     5461 ccataacttt tccattttac cttatcaaaa taaaacagat ataaaaaaga atagcaaata
     5521 aaagccccac aagcaacaat cagaaatgag gtttccagag agaactcccc cttcataacc
     5581 ccccaaagac aaaaaacaaa gacttaaata aaagtaacct actgaggaaa aaagtgtctc
     5641 tcagtggatt cccaattgag gatttcagaa atggtgaaaa aggagacatt attcctgaat
     5701 ggttctgtca tccttttgtc gtcacccctg agtgcctcta ttgcccctta tccgtcaccc
     5761 ctgagtgttt ctacgagggg tccacagtat ttaaaagcct ttggattccc gattaaagat
     5821 ttcgggaatg acggcggggg catcactcct gaatggttct acgaggagtc tacagtgctt
     5881 aaaagctttt ggattcccga taaaatattt caggcataaa acaaaataaa gggaatattc
     5941 tcacacccgt catttctgaa tgcctcactg tagaaaaaac ctcttactac ccagagggag
     6001 aaaaaaagag attgccacac cagcgatgtg acgggattta cgataacaca cgagggaagc
     6061 cagccatctg aggattgaac agaaaggtaa agaacattat aatagttttt gtgattaact
     6121 accataatcg gggggaatac ctaaaaggag gcgaaaatca tgcccaatat taccgttgaa
     6181 gggccaccaa tttctgatat tgaaaaaaag agaaaactgg tacaaaccat aacccaggta
     6241 gctacagaag tatacggatt acctgctcaa gctattgtgg taaccatccg ggaaaattct
     6301 ccagaaaacg tagcaattgg aggaaactta ttgtcggacc agaaaaaaag ctgagaatca
     6361 ccgggtaatt atacccggtg attctccaaa caccttgctc ttagaaggtt aaaaatactc
     6421 cccactattt taagccccaa aatcgctcag gaaagagaaa aatcttttag aaatttctcc
     6481 atccctattt tagaattagt agcgagaata gaacggctcc tgacgttcca ttctttgttt
     6541 cccagctccc tgacttctcc tcctgcttct cgtaccaaac ataccgctgc tgctacatca
     6601 taaaaagata gtcccagctg taaatatcct tctaagcggc cgcaggctac ataagccacc
     6661 cccagagcag cgcttccgaa agagcgcaga gcctggcact cggcagcaat ctgagcataa
     6721 acaggctcca taatctgcca cttagcactc tggtgaggaa agcctgttcc cacgatagtt
     6781 cggtgcatct cttttatatc tgagactcga attctttctc cattcaggaa agctcccttc
     6841 cctttttctg cccagaaagt ttctcccaga cgaggaaagt ccaaaacccc tattatcggc
     6901 tccccttctc tgagataagc aatagaaata ccataaaggg gtaaattatg aatgtagtta
     6961 ctggtaccat caatggggtc aatcacccag gtagaggaaa aatcttccag aggataatta
     7021 ctttcctctc cccagaaaag gtcttgagca aaatgagaag atagtccctc aattatcatc
     7081 tcctgagctt ctttatctac ttcagtaacc acatcaaaag gactgttttt ctcttttatt
     7141 tcttgaatat ggttctcgtg gtccatcaca aatttcccta cttctttagc cacatctaaa
     7201 gcaaccgaaa gacgagtaat cactctcaat ctcccataca agtccctaaa gagcgagcta
     7261 tatctaagag aggtgaatcc agcggaacaa ctttcaagtt ggatatggcc acttccatag
     7321 gcaaaggaac aatctcttct ccctggaggg ccgggaaata accatatttt ccttctgcta
     7381 ttaaatctac ggctttggct ccaaagcgag tagctaaaat ccgatcaaaa gccgtggggg
     7441 aaccccctct ttgcagatgt cccagcacta ctgttcttgt ctccaggcca gtttttcttt
     7501 ctatatcctg ccctacttta tatccgatac ctcccaaacg aatctgatcg tggctttcct
     7561 ctactatttt ttgcaccacc atctctcctc ctttaggacg ggctccctct gccaccacta
     7621 caatactgaa tctcttccct gctgctcctc ttttcaggat gaattcacag actttgtcta
     7681 tgtcgaaagg aatctcagga atgagaatga tatctcctcc tcctgctatc cctccataaa
     7741 gagcaatcca tcctgcgtat cttcccatca cctccaccac cataacccgg tggtgagatt
     7801 gagcagtagt atgaagacgg tcaacagcat cagtaaccac cgctactgct gagtcaaagc
     7861 cgaaggtcaa atcagttcct cccagatcgt tatcaatggt cttgggaact cccactatgt
     7921 tcaagccttt ttctgcaagc ttttgagaaa cattaaaagt cccatccccc ccaatggcaa
     7981 ttagagcttg aatgtccatt tgatggtaat tatcaatcac cttctgagaa aaatcacgaa
     8041 aaactaactt tccttcttca tgcaccgggt agcgaaaagg gtctgcttta ttggaggttc
     8101 ccaaaatagt ccctcccagg ggcattatcc ctgaaacgtc attgtattct aactttctcc
     8161 atttcccttc taccatcccc tcaaaaccgt ctaatacccc tacaacttcc cagccatagc
     8221 ggagaacggc tctttttacc acagcatgga taactgcatt aataccgggg caatctcctc
     8281 ctccgctcaa aacccctatt cttctaactt ccactatttt ttcctcctca tcatagctaa
     8341 aaaccgggac agagtcatgc tcgccactat tcagccatct ctgaagttct ccaatactaa
     8401 atcctggcat ttaaactaaa attacactat atagggctcc agcagctgtt tagccagtaa
     8461 aatacctcgc aatttgcttt cctggtcttt ttcaaagctt ctatcttcgt gttcgatact
     8521 caccacatag ttatagccca cttcataaag agcgttaatg aaagagttcc aatcgatatc
     8581 tcccgtcccg gccactttat ctatatacca gccaaaacca aagattccta cttcgtctaa
     8641 cctattccaa tcaatacgag tgtctttagc atgaacatga aagatttttt ctttaaaatc
     8701 ataaactgct ttcacataat caatttgctg ccagatgaga tgagaagggt caagattgag
     8761 accgaaataa tctgagggaa tcatctcaaa cattcttctc cagatctctg gagtagtagc
     8821 taaatttgtc cccccaggcc agcggtctaa actatagagc atggggcagt tctctatggc
     8881 caattttacc ccgtggtctt cagcaaattt aactaaatca ggaaaaacct ttttaaactc
     8941 ttccagagat tcttccacat tcatgcgagg atctctccct acaaaagttc ccaccacttt
     9001 tacttccagc ttttctgcag cctgaataac tttcttcaaa tgttcatggt aaaaacaacg
     9061 actttccaga tcgggatcca ggttattggg ataatagcct aaagaagata tctccaggta
     9121 attactctgg aatagttctt ttatctcctg tgctttttct tcagtaaaat tttctacgtc
     9181 gatgtgctga gtaccagcat atcttctctt ttcaaaaact cgaggccagc aagccacttc
     9241 taacatacca aaaccttttt cctttgccca cttaactatc tcttcaagct ccatttccgg
     9301 taaacaagca gtcataaaac ctaatctcat ttgctcaccc tctttttagt taactctaat
     9361 ccattttccc tctttagcac tgcggaggat agcttcgcag agaacaactt ctctaagtcc
     9421 atccgtgaaa gtgggaaaag aaaaatctcc tcttttccct tctaaaatac actgataaat
     9481 ttcacggaaa agctgtttag aagtatcggg aaatccttca ttatgtcctc cagggaaaga
     9541 aatgattttc cttgcttctt cactgaccaa ggaaggatcc tttaaaagca attcattagc
     9601 tcgatctctt cttcctatcc acaactcatt aggtctttcc gaatcccaga atatagccga
     9661 gcgtgaacca tcgatttcaa aagagcaccg attttt
//
LOCUS       DAOPSS010000056         9575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_5987_length_9575_cov_6.426366, whole genome shotgun sequence.
ACCESSION   DAOPSS010000056 DAOPSS010000000
VERSION     DAOPSS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9575
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5987_length_9575_cov_6.426366"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="PKN31_04710"
     tRNA            complement(1..76)
                     /locus_tag="PKN31_04710"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Met,seq:cat)
     gene            complement(256..330)
                     /locus_tag="PKN31_04715"
     tRNA            complement(256..330)
                     /locus_tag="PKN31_04715"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(295..297),aa:Glu,seq:ctc)
     gene            complement(721..1356)
                     /locus_tag="PKN31_04720"
     CDS             complement(721..1356)
                     /locus_tag="PKN31_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOA99260.1"
                     /translation="MKLVKDSISEQIYSIIKEEILLAKRKPGEQINPRKLADEFETSV
                     MPIRDALNQLANEGLVIRKPRVGFFVRSFTPKEAKDLMEVRKLYELYCLEKYFDKIDR
                     KKLSDYLDRSLEKIDISYKDREFDELDENIHDLIVKASNNSWLINGYNSIKNYIVVLK
                     HLDTGQVQAAQREHISLINAILDGDKEKAMKVLEEHIQRVTEELMMSLISQ"
     gene            complement(1550..2368)
                     /locus_tag="PKN31_04725"
     CDS             complement(1550..2368)
                     /locus_tag="PKN31_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOA99261.1"
                     /translation="MKVNFDLKGKAAVITGGGGILCSEMARGLASWGVQVAVLDIRED
                     SALKVAKEIEQSGGEAIAIKADVLNKNSLLEARSRVLEEYGKIDILINGAGGNRKEAT
                     TSEEQSFFDLPEDALQWVFNLNFLGSVLASQVFGEAMVENKTGSIINISSMAAFRPLT
                     KTIAYSAAKAAVTNFTQWLAVHLNQNYSKNLRVNAIAPGFLITEQNRYLLIDERTGEE
                     TERGRRIKDHTPMGRYGEPQELVGAVIWLCSEAASFVNGVVIPIDGGFSAYSGV"
     gene            complement(2396..3136)
                     /locus_tag="PKN31_04730"
     CDS             complement(2396..3136)
                     /locus_tag="PKN31_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA99262.1"
                     /translation="MEGKIAIVTGGASGIGKAIADRLADRGYTVVVCDINEEKGRALE
                     KASHKVKGTYVFKYCNVGEEEDIRRVVEEVEREYGGISVLVNNAGIIRRRKGEEIKIS
                     DWDDVFKINTRGAFIFCKYVSEVMKKQKSGRIINISSVASKMGDITSAPGYGPSKAAV
                     NGLTKTFARELAPYNITVNAVAPHAIETEMSAEWSEEMRQKIISEIPLKRLGQPSEVA
                     AVVEFLVSDEAGFITGEIIDVNGGFLMD"
     gene            complement(3164..4450)
                     /locus_tag="PKN31_04735"
     CDS             complement(3164..4450)
                     /locus_tag="PKN31_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOA99263.1"
                     /translation="MALVLIVFLVLLLLGMPVAFAIGISGFLFFVQQPALPLTMPVQL
                     ILSGTQSFSLLAIPMFIFAGNLLNNTGITSRLVQFASVLTGHMRGGLAQTSVVLSTLM
                     GGVTGSAIADASMEARILGPAMTKRGYDRAYSAVVHGFTSLITIAIPPGIGLVLYGSI
                     GEVSIGRLFAGGILPGLLMAALFMIVVSITSGKKGYLPEREKRASAKEIIPAFISSLW
                     AIFFPIFLLVALRFGFLIPSEAGAFASVYALAVGLIAYRELTWKKFLSAVKYTIMDVG
                     MIMLLIALSSLVSYGMTWEMIPQTLSQLLLGISENPYVITGLIVLMLLVLGMFIDSTV
                     LILLLTSILVPVMKQIGMDLVHFGVLMVITCATGLLTPPVGLVMYSVCSIMGCSLEDY
                     IRECWPFLIAMFILIALMLFFPQIILFIPNLIFGKA"
     gene            complement(4490..5014)
                     /locus_tag="PKN31_04740"
     CDS             complement(4490..5014)
                     /locus_tag="PKN31_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOA99264.1"
                     /translation="MMTIKKIYDLICRGEEIVAGAFMVAIMVIVFAAGFGRTIGYPIQ
                     WGMEMSTFLFAWVVFLSADAAWRKNKHVSVEVLVSKLPTKLQYYITLFNHCLIFGFLL
                     LLVGYGIPLCYHTRFRTFQGIPGFSYMWATLSVPVGSALLSITAVLKIRELVKHGKVA
                     IFETGNEGDMDIDI"
     gene            complement(5130..6119)
                     /locus_tag="PKN31_04745"
     CDS             complement(5130..6119)
                     /locus_tag="PKN31_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014514532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HOA99265.1"
                     /translation="MLKKLTGIIIVLAMVVGFWASVALAQEQYVLRFNHVLAPGHPYH
                     EGFQKWAERVYERTNGGLKIEVFHSAQLGVEEDIIEQIRQGVPVGQNTDAARLGNYVP
                     EIAVINGPYFVESIEEVQKLHESPSIQEWIDKLDKEYGLKVLSFNWVQGYRHFMTKDK
                     EVRTPEDLKGLRIRTAPAPIWQESVRALGATPVALNFDEMYTALQQGAIDGAELVYDN
                     VTSGNLYEVLNYISETRHFLLVNFEVISSKWFYSLPEEYQKILVEECDRAGLETSYLI
                     EENTEKTKEIIREKGVTIIEDVDLEAFRKAGEAAYEVLGISEAKEKVYQEIGK"
     gene            complement(6419..7453)
                     /locus_tag="PKN31_04750"
     CDS             complement(6419..7453)
                     /locus_tag="PKN31_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA99266.1"
                     /translation="MSHKQIGFAIVGYGLAAKLHAQALTQLDEAKLIAIVGRNAKKAS
                     ELAENFGVEALSLDEALRREDIQAFIVATPTGSHLEVVKEIAPRGKHLLVEKPLESNL
                     QRTDELIRTCEEAGVILGAVFQHRYDDASLMLKEYKERGLLGRTVMGSAYVKWYRTQE
                     YYDTRPWRGRVETSGGGALATQASHSIDLLLWFLGEPEKACGFMNTLAHTGIEVEDVA
                     VASIQFKDGALGSIEASTATYPGFPERLEFHFERGSAIIEGGKIIYLNTLSEEIPVIE
                     RGQGGLSGASDPAGVDIQPFVRMLQDFVNALREGKNPPLDGREGRKAVELIEAARISA
                     LESRVVELPL"
     gene            complement(7465..8592)
                     /locus_tag="PKN31_04755"
     CDS             complement(7465..8592)
                     /locus_tag="PKN31_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOA99267.1"
                     /translation="MLEKNNLDSSVKQEIQEKLVRLRKLIEGKNLKALLLKRHVNFSW
                     LTAGGTNVLCMAQDTGVSSILVTDDRSYVIVSNIEAPRMKEEEKVVAKGFELVEYPWY
                     EENEMKIVREICGEGSIGCDITFPGTQDLSGEINRCRYQLLGTEKERYLWLGKEVSWT
                     LEEVLCSLHPGVLESEVAAEIALRLWKKRIEPIGLQVAADERAYRFRHPVAFRNPARR
                     LLLASVMARYGGLVTTVTRMVYFGTPSPEFLKQYRDNVRIECEMIALSRPGNKAVLPL
                     QRAVELYQELGYPEEWKRHHQGGAMGYLPRDYRVDFECQENILENQAFCWNPSIAGTK
                     SEDGFISTSHGPLFITYPVRFPVFNLEVENFHFERPDLLLL"
     gene            complement(8608..>9575)
                     /locus_tag="PKN31_04760"
     CDS             complement(8608..>9575)
                     /locus_tag="PKN31_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOA99268.1"
                     /translation="GLISALLGLFLGTMGMDPISGYSRFTFGSYALEGGLPLLPVMIG
                     LFAVSQVLRDIEEAGVIYQVVNKIGRIWPSREELKRILGASLFGSTLGTLIGAIPGIG
                     GSIATFLAYGQYRKFSKRGEEFGSGVLEGVAVPESANNATTGGALIPLLTLGIPGDIV
                     TAVMLGILILFGVNPGPMLFREQPEVVNRLFASMFVIAFFILVLGLFGARFSPKILAI
                     PKKILSPLVLLLCVVGAYADSSSAFGITVALVFGVIGYFMEKFDFPVAPLLISMILGP
                     IAEGELGRSLLISRGDWSILFVRPISLCFLILAGLSLFFAFRKKD"
BASE COUNT         2924 a         2349 c         1589 g         2713 t
ORIGIN      
        1 tggtggcggt gcggggattc gaaccctggc actccgggta tgagccggat gctctgacca
       61 tctgagctac accgccatcc aatagaatat tataactgtt ttaccacaaa agacaagaat
      121 gccaatggaa tcatttaaaa cttatacttt actcataaac aaccgcctct ctctctggaa
      181 gtagatttat agtcgtcgga ctcagaagtt ttttagagag agtgaggaag ctgcgaaaag
      241 gctctgtgaa gcctttggtg gcccaacggg gaattgaacc ccggcttgcg gattgagagc
      301 ccgctgaact aaccactatt cgattgggcc acacatttat tatatttcct aagaaaggtg
      361 ctgtcaacgt ctacgaaggc tacttgaaaa ttctttcttt atagtcacca aaaaccccag
      421 tgatttctat tctttgcacc tgatttcttc ttttgccaca gcgttgttag ataataacat
      481 cattttttct ctagtggaaa agaaatttta taagcctcta taaagatatt taaatttgag
      541 atagtagcac atacttagaa agaatggtgt ttcttatcca attcttctcc ggttaacaaa
      601 ataattctgc aaagaaaaag agaatagata ggcacccttt accccctgcc ttctatctta
      661 gtcataaatt ggggagaaca atgtccaaac cttatgggcc tcagtatagc tttcatgata
      721 tcattgcgaa atcaggctca tcattaactc ttcagtcact ctttgtatgt gctcttctaa
      781 aaccttcata gctttttctt tgtctccgtc cagaatagca ttgattaaag atatgtgttc
      841 tctttgagct gcttgaactt gaccggtatc taggtgtttg agaacaacaa tatagttttt
      901 aatgctatta taaccgttta ttagccacga gttatttgaa gctttaacaa ttaaatcgtg
      961 gatattctcg tccagttcat caaattctcg gtccttataa gaaatgtcta tcttttctaa
     1021 agatctatct aaataatctg acaatttttt acgatctatt ttatcaaagt acttctctaa
     1081 acaataaagc tcgtaaagtt tccgcacctc cataaggtcc tttgcttctt ttggggtaaa
     1141 agaacgaaca aaaaagccaa cccggggctt acggatcact aacccttcat tagctaactg
     1201 atttaaggca tcccttatgg gcataacact agtttcaaat tcatcggcca gctttcgggg
     1261 attaatttgc tcccctggct ttcgcttagc aagcagtatt tcctctttta ttatagagta
     1321 tatctgctcg ctgatgctat cttttacaag tttcaattta cgatttatcc tcctctcaaa
     1381 ttcaacttca tgcatcttaa gaaaatgagg caatggtaat caaagcctat ttagtatttt
     1441 tttctgatac cggtaaacaa actgttagca caaatctaaa taagccattg accaaaaccc
     1501 catctcactt ctatcaatct ctctactttt tgggccttct tccaacctct taaactcctg
     1561 aataagccga aaatcctccg tctatgggaa taaccactcc gtttacgaaa gaggctgctt
     1621 ctgagcaaag ccagataacc gcccctacta attcttgtgg ctctccatag cgtcccatag
     1681 gggtatggtc tttaattctc cgcccccttt ctgtttcctc accagttctc tcatcgatta
     1741 ataaatatct attttgttca gtgataagaa aaccaggagc gatagcatta acccgcagat
     1801 tcttagaata gttttgatta agatgaaccg ccaaccattg agtaaagtta gtaacggctg
     1861 ccttggctgc agaataagca attgttttag tcaaaggcct aaaggcagcc atggaagata
     1921 tgttgataat tgagccagtt ttattttcca ccatagcttc tccaaaaacc tgagaagcaa
     1981 ggactgaacc cagaaaattc agattgaata cccactgcag agcatcttct ggaaggtcaa
     2041 aaaaactttg ctcttcactg gtcgtggctt cttttctgtt accacccgcc ccgttaatta
     2101 aaatatctat cttgccgtat tcttctaaca ctcttgagcg agcttccaaa agggagtttt
     2161 tattcaatac gtcagcttta atagctatcg cctcaccacc gctttgctca atctctttcg
     2221 ctactttcaa tgctgaatcc tctctgatat ccagaactgc tacctgaact ccccatgaag
     2281 ctaaaccacg agccatttca ctgcaaagaa ttccgccacc accagttata acagctgctt
     2341 tccctttcaa atcgaaattt accttcatta tttgttatca cctcttttaa tttccctaat
     2401 ccattaaaaa gccgccatta acgtcgatta tctctccggt aataaaacca gcctcgtcag
     2461 aaaccaaaaa ctccacaact gctgccactt cagatggttg acctaacctt ttgaggggga
     2521 tttcagaaat gattttctgc ctcatctcct ctgaccactc agcgctcatt tcagtttcaa
     2581 tggcgtgagg ggcaacagca tttaccgtta tattataggg ggctaactcc cgggcaaaag
     2641 ttttggtaag gccgtttact gccgccttag aaggaccata tccaggagct gaggtaatat
     2701 cacccatttt gctggccaca gaagaaatat ttattattcg gccacttttc tgttttttca
     2761 taacttcaga aacgtatttg cagaaaataa aagcaccacg agtattaatc ttaaaaacat
     2821 cgtcccagtc agatattttg atttcttctc cttttcgtct tctgatgata ccagcgttgt
     2881 taaccagcac tgaaattccc ccgtattctc tttcaacttc ttctactact cttctaatat
     2941 cttcttcttc acccacatta caatatttga aaacataagt tcccttaacc ttgtgactgg
     3001 ccttctccag ggcccttcct ttttcttcat taatatcaca tactacaacc gtatatcccc
     3061 tatcagctaa gcggtcagca atagctttgc cgataccaga agcaccaccg gtaacaattg
     3121 ctatctttcc ttccataaac catacctcct gaaaaatctt cgtctacgct ttaccaaata
     3181 ttaagttggg aatgaataaa ataatttgag gaaagaataa cataagagca atcaaaataa
     3241 acatagctat taaaaagggc cagcattccc ttatataatc ctccagagaa catcccataa
     3301 tggaacaaac agaatacatg actaaaccaa ctggaggagt taaaagtcct gtggcacaag
     3361 ttatcaccat caaaactcca aagtgaacca ggtccatccc tatttgtttc attacgggca
     3421 ccaatattga agtgagcaat aaaatcagaa ctgttgagtc tataaacata cccagcacca
     3481 aaagcatcag cacaattaga ccagtaatta cataaggatt ttcactaatc cccagcaaaa
     3541 gctgggagag agtctggggt atcatctccc aggtcatacc ataactaacc agagatgata
     3601 aggcgatcaa aagcataatc atacccacat ccattatggt atatttaaca gcagaaagaa
     3661 attttttcca ggtaagctcc cgataggcta tcaagccgac agccagagca tatactgagg
     3721 caaaagcacc cgcttctgaa ggaattaaaa acccaaaccg caaagctacc aaaagaaaaa
     3781 tcgggaaaaa tattgcccag agactactaa taaaagctgg tataatttct tttgcagaag
     3841 ctcttttttc tcgctcggga agataacctt ttttacccga agtaatggag accactatca
     3901 taaacaaggc cgccattaac agtccgggta aaataccccc ggcaaacagt cgccctatgg
     3961 aaacttcacc aatactccca tataacacaa gtccaatccc cggtggaatg gctatagtta
     4021 ttagagaagt aaaaccatgc actacggctg aatatgcccg atcataacca cgtttagtca
     4081 tcgctggacc taatatcctt gcctccatag aagcatcagc gatggccgaa cctgtaactc
     4141 ctcccatcag agtacttaat actacactgg tttgagctag accaccccgc atatgaccgg
     4201 taagtactga ggcaaattgc accagccgac tggttattcc agtgttgttt agcaggttac
     4261 cggcaaagat aaacattggt attgccagca gagaaaaact ctgggttcca gatagaataa
     4321 gctgaaccgg catggttagc ggcaatgccg gctgctgaac aaaaaacaaa aatcctgaaa
     4381 tacctatggc gaaagctacc ggcatgccca acaacaagag aacaagaaac acaattaata
     4441 ccaacgccat gttttttcct ccttatcagt aagaaacaat agtatattgc tatatatcta
     4501 tatccatatc tccttcattc ccagtttcaa aaatcgcgac tttcccatgc tttacaagtt
     4561 ctcttatctt taaaacggcc gttattgaca aaagggcact acccaccggc acactaagag
     4621 tagcccacat atagctaaat cccggaatac cctgaaaagt ccgaaatctg gtgtgatagc
     4681 aaagggggat gccataccca accaaaagca ataaaaatcc aaaaataaga cagtgattaa
     4741 ataaggtaat atagtactgg agttttgttg gaagcttgct gacaagaacc tccacactca
     4801 cgtgtttgtt ttttctccag gcagcatcgg cacttaaaaa gaccacccag gcaaaaagaa
     4861 aagtagacat ttccatcccc cattgaatgg gataaccaat agttctccca aaccctgcag
     4921 caaagactat taccataatc gcaaccataa aagctccagc tactatttct tcccctctac
     4981 aaatcaagtc atagattttt ttgatggtca tcattaatac cccacattaa atatttttat
     5041 taccctattt acaattaatt tccaaatctg tttatgtctt attaccccca agccaagttg
     5101 gcttgggggt aataacaatc tttaacctac tactttccaa tttcctggta aactttctct
     5161 ttggcttcac ttatgcctaa aacctcatag gctgcttctc cagctttcct gaacgcttcc
     5221 agatcaacat cttcaattat agtcacgcct ttttcccgaa ttatttcttt agttttctca
     5281 gtattttcct caatcaagta agaagtttcc agacctgccc ggtcacactc ttccactaaa
     5341 attttttgat actcctcagg aagactgtaa aaccatttgc tgcttattac ttcgaagttt
     5401 accagcaaga aatgacgagt ttcactgatg tagttgagaa cttcataaag gttacctgag
     5461 gtgacattat catacactaa ttctgctccg tctattgcac cctgctgcaa ggcagtatac
     5521 atttcgtcga agttcaaagc aacaggggtc gcccccaatg ctcgaacaga ttcctgccag
     5581 ataggagcag gtgcagtccg gatacgcaga ccttttaaat cttcgggagt tctaacttcc
     5641 ttatccttag tcataaagtg cctgtaacct tgcacccaat tgaaggacag cactttaaga
     5701 ccatattcct tatccagctt gtctatccat tcctgaatcg aaggggattc atgaagtttt
     5761 tggacctctt caatactttc tacaaaataa ggaccattta ttaccgcaat ttcaggtaca
     5821 tagttaccga ggcgagcagc atcagtattt tgtcccaccg gaacaccttg acggatctgc
     5881 tctatgatgt cttcttcaac tcccaattga gcactatgga aaacctcaat ctttaaaccc
     5941 ccattggttc tttcatatac ccgctcagcc catttctgaa acccttcatg ataagggtga
     6001 ccgggagcca acacatgatt aaacctcaaa acatattgtt cctgagccag agcaactgaa
     6061 gcccagaatc ccactaccat cgctaacaca atgataattc cagttagttt tttaagcatc
     6121 ttagtaaacc tccctttttt aaattaatgt taaatcaata agaaactcca aaataagaac
     6181 ttaagaacgt ttaccatcta tgctttaatc acccccttaa tttatggaaa atccctattt
     6241 tgcccaaacc agcaattttt caattgttaa agaaaccatt agattgtgac atgtaatacc
     6301 tgatatatca catcctaatt ttatcaaacc tggcaaaaaa ttcaatatct aatttataaa
     6361 cattgtttta tgcctaataa atatttcttt actaaattta ataggatgac ttatcttgtt
     6421 agagaggaag ttctaccact ctgctctcca gagcagatat tcgtgctgct tcaatcaatt
     6481 ccactgcttt tcttccttct cgaccatcca aggggggatt cttaccttcc ctcagagcgt
     6541 taacaaagtc ctggagcatt cttacaaaag gctgaatatc cacaccagca gggtcacttg
     6601 ctccagatag gccaccttgt cctcgttcta taactggtat ttcctcagaa agagtattaa
     6661 gatagattat cttgcctcct tcaatgatgg cactccctcg ctcaaaatga aactccagtc
     6721 tctccggaaa accaggatac gtagctgtac tagcttcgat gctacctaga gctccatcct
     6781 taaactgaat agaggccact gccacatctt cgacttctat tcccgtatga gccagagtat
     6841 tcataaaacc acatgctttc tcaggttcac ctaaaaacca gagcaaaaga tctatactgt
     6901 gtgaagcctg agtggcaagt gccccaccac cagaggtctc tacacgtcct ctccagggcc
     6961 gagtatcgta atattcctgc gtccgatacc actttacata agcacttccc attactgttc
     7021 tacccagaag ccctctctct ttatactctt taagcataag agaagcatca tcatagcgat
     7081 gctgaaaaac agcaccaagg ataacccctg cttcctcaca ggttcggatt aattcatcgg
     7141 tacgttgtaa attgctttct agaggctttt ccaccaagag gtgtttacct cgaggagcaa
     7201 tttctttaac tacctcaagg tgactacccg taggagtagc tacaataaaa gcctgaatat
     7261 cttcccttct caaagcctca tcaagggaaa gagcttctac tccaaaattt tcagctagct
     7321 cggaagcttt tttagcattc ctacccacta tagctattag ttttgcctcg tcaagctgag
     7381 taagagcttg agcgtggagt tttgcagcaa gcccataacc aacaattgca aatcctattt
     7441 gcttatggct catagctcac cctcctaaag caaaagcaga tctggtcgct caaaatgaaa
     7501 attttccact tctaggttaa aaaccggaaa acgtaccggg taagttataa atagcggacc
     7561 atgagaagta gaaataaaac catcttcgga tttagtacca gctatagatg ggttccaaca
     7621 aaaggcctgg ttctcgagaa tattttcctg gcactcaaaa tctacccggt aatctcgagg
     7681 caaataaccc atcgctcctc cctgatgatg acgtttccac tcctcaggat atcccagctc
     7741 ctggtaaagc tctactgctc tctgaagcgg tagtacagct ttgttaccag gtcttgagag
     7801 agcgatcatc tcgcattcga tacgtacatt atcacgatat tgtttcaaga attctggcga
     7861 aggtgttcca aaataaacca tgcgggttac cgtggttacc agaccaccat atctagccat
     7921 gacacttgcc agcaataacc ttctagccgg gtttcgaaaa gctaccgggt ggcggaaccg
     7981 ataagcacgt tcatcagcag ctacctggag tccaataggt tcaatccttt tcttccagag
     8041 tctgagagca atttctgcag ctacctccga ttcaagcaca ccaggatgca aactacacag
     8101 tacttcttcg agtgtccagg aaacttcctt acctaaccag agataacgtt ccttttctgt
     8161 tcccagaagc tgataacgac agcgattaat ttctcctgat aaatcttgcg ttcctggaaa
     8221 agtaatatca caaccaatac taccctctcc gcaaatttct cgcacaattt tcatttcatt
     8281 ttcttcatac cagggatact ccacaagttc aaaccctttt gcaactactt tctcttcctc
     8341 cttcatccgg ggggcctcaa tattactgac tataacatag cttctgtcat cagtaaccaa
     8401 gatgctactc accccagtat cttgagccat acagaggaca ttagtcccac cggcagtcaa
     8461 ccagctgaaa tttacatgcc tttttaaaag taaggccttc aaattttttc cttctataag
     8521 tttgcgaaga cggactaact tttcttgaat ctcctgcttt acggaagaat ccagattatt
     8581 tttctccaac atagtctcca cctctttcta atcttttttt cgaaaggcaa agaacagcga
     8641 taatcctgcc agaatcagaa aacaaagcga aataggacgg acaaatagta ttgaccagtc
     8701 accccttgaa atcagaagac tccgacctaa ttctccctca gcaataggac caagaatcat
     8761 actgataagc aatggagcca ctggaaaatc aaacttctcc ataaaataac ctatcactcc
     8821 aaataccaga gcaactgtaa ttccaaaagc acttgaacta tctgcataag ccccaactac
     8881 acataacaaa agtactaaag gagaaagaat ttttttggga atggcgagta tttttggaga
     8941 aaatcgcgct ccaaacaacc ccaacactaa aataaaaaaa gcaatcacga acatggatgc
     9001 aaaaagccta ttaactactt caggttgttc ccggaagagc atgggtccag gatttactcc
     9061 aaaaagaatt aagattccca gcatcactgc ggtaacaata tctccaggga tacccagagt
     9121 taaaagagga ataagtgctc ctccagttgt ggcgttattg gccgattctg gaacagcaac
     9181 tccttctaac actccgctac caaactcttc tcctctttta gagaacttgc gatactgacc
     9241 atatgctaaa aaagtggcta ttgaaccacc aataccggga attgcgccaa ttaatgtacc
     9301 cagcgttgat ccaaagagag aagcccctaa aattcttttc agctcttccc gggaaggcca
     9361 aattcgccct atcttattaa ccacctggta aattactcct gcttcctcta tgtctctcaa
     9421 cacttgagaa actgcaaaga ggccgatcat tacgggcaaa agtggtaaac ctccttcgag
     9481 agcataagaa ccaaaagtaa accgggaata acctgatata ggatccattc ccatagtacc
     9541 cagaaaaagt cccagcagag cagagattaa acctt
//
LOCUS       DAOPSS010000057         9523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6023_length_9523_cov_7.913921, whole genome shotgun sequence.
ACCESSION   DAOPSS010000057 DAOPSS010000000
VERSION     DAOPSS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6023_length_9523_cov_7.913921"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(601..1539)
                     /gene="rbsK"
                     /locus_tag="PKN31_04765"
     CDS             complement(601..1539)
                     /gene="rbsK"
                     /locus_tag="PKN31_04765"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOA99269.1"
                     /translation="MVTPTVAIVGSNMMDLITYYRDRMPKTGETIFGEDFEIGFGGKG
                     ANQGVAVSRLGGKVIMVTAVGDDLFGPLVKNNFLENGISSKFIKTVPGKTSGVAPIFV
                     DEKGRNSIFIIPGANYSLFPEDVIQAEEELQKASFLLLQLEIPLETVYYAIDMAQKLN
                     LKVILNPAPAQELEWNKIQSVFLLAPNESELESLTGIPADSFTHIQEGASFLLEKGIK
                     NVLVTLGEKGSALFTPQESKEFAAYSVNSIDTTGAGDAFLGALSFFLAEGKDIDEAID
                     LSSAYAALSTTRRGTQKSFASREEFENWLKSYSSGA"
     regulatory      2077..2184
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2235..2750
                     /gene="thiW"
                     /locus_tag="PKN31_04770"
     CDS             2235..2750
                     /gene="thiW"
                     /locus_tag="PKN31_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HOA99270.1"
                     /translation="MVNRAAKISFRKTAYVAIFCALAVVSSGVSFPIGPTKVFPFQHA
                     INVVAGIMIGPWYGGLAAFLAGLLRIAIGTGTIFALPGGVPGAIIVGLFYRWLRRDWV
                     ALTEPLGTGFIGAGLSAYLVSPFTGGEATFFFFQSAFLVSSIPGCIIGLLFVRVLRKA
                     ISVDTWEEIGG"
     gene            2753..3994
                     /gene="cytX"
                     /locus_tag="PKN31_04775"
     CDS             2753..3994
                     /gene="cytX"
                     /locus_tag="PKN31_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HOA99271.1"
                     /translation="MSEDIQPVPQQERNLSGMDFFVLWMGAAIAISEVWAGGMLAPLG
                     LIGGLWAIILGHVIGNTPLALGGVLGSDSGVPAMVSVRAAFGKLGSYLASALNIIQLA
                     GWTAIMIIICAQSVDNITQILAGYTNVKLWMLIAGIISTFWALVGTPLWKWMQRIAVF
                     SLAILCLFMTYSVLHNVSTEELLNWRGDGSLPWGAGLDLVIAMPISWLPLVSDYSRFA
                     RESRSAFWGTWWGYFLASSWMYVLGLLLALSTGQANPIPVMLALNWGIIALVIVFFST
                     FTTTFLDIYSGAMSFLNLFPRFQEKLITLFFGLGGTILALVFPMDRYESFLYLIGGIF
                     IPLFGVVIFDYFFYRRRKIEIKELYEGKALDRGALLSWVGGLMIYIIFLRFLPHWGAS
                     FPSFLASGLIYLGTRRKFNDL"
     gene            3984..4790
                     /gene="thiM"
                     /locus_tag="PKN31_04780"
     CDS             3984..4790
                     /gene="thiM"
                     /locus_tag="PKN31_04780"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOA99272.1"
                     /translation="MTYEEKCTLIFKKIKEEKPLVHHITNFVVINDNANTMLAIGALP
                     VMSYAREEVEEMVALSQALVLNIGTLTPETVESAILAGRKANELGIPVVLDPVGAGAT
                     QFRTESALKILQEVKISILRGNTGEIGNLLGIKAEVRGVEGGVVDADTSDLAQRASQK
                     FQTVVAITGKIDVVSDGKRVVKIKNGDILLTRITGTGCMATSLCAACAAVERDYLWAA
                     SSALGFLGVCGEIAARHSLGPGSFRVSLFDTFYGIKKEELKALLSLEVKD"
     gene            4787..5422
                     /gene="thiE"
                     /locus_tag="PKN31_04785"
     CDS             4787..5422
                     /gene="thiE"
                     /locus_tag="PKN31_04785"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOA99273.1"
                     /translation="MKTIDWSLYVITDRKIQKKSNEEVVEEATDAGASVVQLREKDLP
                     TRLWVEEAQKVREVTHRRGIPLIINDRIDIALAVDADGVHLGQEDMPLALARKIAPSL
                     IIGYSVSTVEEALEAEREGADYLGAGTVFPTSTKKDAGEPIGLERLREIKKAVNIPVV
                     AIGGINLSNLEEVLATGVDGVAVVSAIVGAESVKEATRSFRQKIELFRRRP"
     gene            5419..6423
                     /gene="thiL"
                     /locus_tag="PKN31_04790"
     CDS             5419..6423
                     /gene="thiL"
                     /locus_tag="PKN31_04790"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOA99274.1"
                     /translation="MKLKEWGEFGIIDYLKNFFPSRGKRVVLGIEDDCAVVRGPRGFF
                     YLLTCDSQVENVHFILSLFSPFSLGKRVLAVNLSDIAAMGGKPLFALLSWVLRPDMEA
                     EWLKEFMRGIKEEAEAHGVDIIGGNLSRTESQMVFDLTLVGEVENDRPLLLRRNARPG
                     DLVLVTGTLGDSRAGLEIALRGSEEEKEKYSFLLQRFFTPSPRLQVGRLLHSLGERVA
                     TIDVSDGFSQDLGHIVEESEVGAVVEVDKLPISPSLREWSEEENKDPFLFALSGGEDF
                     ELIFTVPRERGKEVKEFIEIESGVPVTIVGEVVSQGGVSLRRDGREEKEVASGWNHFQ
                     "
     gene            6437..7264
                     /gene="thiD"
                     /locus_tag="PKN31_04795"
     CDS             6437..7264
                     /gene="thiD"
                     /locus_tag="PKN31_04795"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOA99275.1"
                     /translation="MKRALTIAGSDSGGGAGIQADLKTFCAFGVYGMSSITSITAQNT
                     QGVEAIFDLPPSLVAKQIECVASDIGVDAAKTGMLSNSSIIEAVAEEIKKFNIEKLVV
                     DPVMVSKSGALLLEREAVDALVHKLFPLSFLITPNLPEASFLVGEEIKDISDMEKACI
                     SLKKWGPRYVLLKGGHLPEEEIVDILFDGEEFISFPARRIATKNTHGTGCTYSAAICA
                     LLSLGYSITEAVKTAHEYMIQVITHSLNLGKGFGPVNHLAPLFLREGVNPEEGGKYW"
     gene            7261..7980
                     /locus_tag="PKN31_04800"
     CDS             7261..7980
                     /locus_tag="PKN31_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOA99276.1"
                     /translation="MKESRIGTSGWSYPHWEGVFYPTHLPQNKWLSYYSQFFDTVEVN
                     SSFYHLPRSTTFSKWRDTTPPEFVFSVKASRYITHIKKMREVEEPLAKLYEAVRELGN
                     KLGPLLFQFPPQLKFNREVFSSFVELLSPDFRYVFEFRNESFFTGEVYEILEKKNICL
                     CFSDTPSFPYQEVITSSFIYLRLHGHGSLYTDFYSEEELEGWGAKIRFWLGERDVYCY
                     FDNDYEGFAVKNAQRLREILS"
     gene            8031..9161
                     /locus_tag="PKN31_04805"
     CDS             8031..9161
                     /locus_tag="PKN31_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA99277.1"
                     /translation="MEKRKLGSTGLSISLLGLGGFHLVELPEKEALRIVNLYLDQGGN
                     YLETAESYGSGNSEEKIGKVAKVRRNDFILATKSAQRSKEGITRALEGSLRRLNTSEV
                     DIFFIHGLNRFEELDEISSSQGALEGVEKAKKEGKIRFVAFSNHVTPEVALNALSLYP
                     FDILMIPLNYFDRFNFPGWEEEVLPRAREKRMGILAMKALADGFLWESEREAIRYTLS
                     LGVDSLVMGANTEEYLRRDIEVVENFVPFTPEEMKKVREEAPELGSYVCRQCDKCLPC
                     PLGIDIPGVFLLEGQYDRQMQDGIIRDPAQYALRDRLRFWLGNQDYAQKAYQELNPKA
                     SVCNECGECEPRCPYKIPIIYKLNLAHEKLTDTRPPGYCRIS"
BASE COUNT         2471 a         1775 c         2355 g         2922 t
ORIGIN      
        1 gggagttata tagtttggac ccctcgtaga accattcagg ggtgacggat aagggcaata
       61 gaggcattca ggggtgacga cacggagatt tttcctccgg gttatttttt atctttttct
      121 tttgtgtttt cgtcttttct ggggtttata agggggcaac gccgccttat agtacttttc
      181 tctctgcctg aaatctttta tcaggcatcc atctccctcc gtcattcccg aaatctttaa
      241 tcgggaatcc acgttttttg cattttcttt gggttttaaa tactgtggac ccctgataga
      301 agcattcagg ggcaggctaa aaacggctcg aaagtcgccg gggtgatgga tgaggagggg
      361 ttgttgcagc gacagacaaa agtagcaatt cttcaacccg tcatcgcgag gctgaggaag
      421 atgctctttc ttcctcagta gtttcatctt tatgttctta ttttacgttt ttgatttttt
      481 tctctttcaa gggttgcagg gggtttaccc ctatagttct gtggtgattc caagtcttta
      541 agctccgatg taacttactt ttaaaatttg agcgatttaa caggttttgc gagaattata
      601 ttaggcgcca gaagaataag attttagcca attctcgaat tcttctctgg aagcaaaaga
      661 tttttgagtt cctcgtctgg tggtagagag agcagcgtaa gcagagctca aatctattgc
      721 ctcatctata tctttccctt cagccaggaa gaacgataaa gctcctagga aagcgtcacc
      781 tgctccagtg gtatctatgg aattaactga ataagccgcg aattctttac tttcctgagg
      841 agtaaaaaga gctgagcctt tctcccccag ggtaaccaac acatttttga ttcctttttc
      901 cagtagaaaa gaagctcctt cctggatatg ggtaaatgag tcagcaggaa ttccagtcag
      961 actttccagc tcgctctcgt tgggggctaa taagaaaaca ctttgaattt tgttccactc
     1021 cagttcttga gctggtgcgg gatttaagat tacttttaag ttgagcttct gagccatgtc
     1081 gatggcataa tatacagttt ctagaggtat ttccagctga agcaacaaga aggaggcctt
     1141 ttgaagttct tcttcggctt gaattacatc ttcggggaaa agggaatagt tggctccggg
     1201 aatgatgaaa atactgtttc ttcctttctc gtctacaaaa ataggagcca ctccactggt
     1261 cttccccggg acggttttta tgaacttgct gctaattcca ttttcgagga agttattctt
     1321 aaccagagga ccaaaaaggt cgtctcctac cgcggttacc attataactt tcccacccag
     1381 acgggaaaca gctactccct ggttggctcc ttttcctcca aagccaatct caaagtcttc
     1441 tccaaaaata gtttccccgg ttttgggcat tctatctcga tagtaagtta tgaggtccat
     1501 catattactt ccgactatag ctacagtagg cgtcaccata atacctccct tgataactaa
     1561 gaagttatta tattatgtct gggaaaactg actgattccc tcttaaactg agctcattat
     1621 accatggttt taggagcatt tggagaattt ggagaatagt cgcgctctct ggggtcgttt
     1681 gaatcttgta aggatgtttt tcctctattg ttagcttgag tggattaagt ctttttcctg
     1741 caaaagtgga tagctttctc atcacctcaa attgcctcct tcaccagttc tttttctaat
     1801 agagctcgtt tatcatttat ctattaagtt atttatctaa tctttagcat agcgaaagct
     1861 actttttgcc aatatcttaa ttctttccct ttctttgtat ttctacgaag atatagagga
     1921 taaccatgtt actttttgct acctgccaag ttcctgggtt gtaaagtaat tgttcataac
     1981 aagaagttta ctaaaaactc gcccgagcat agtttcctaa aacctttttc tgcactggag
     2041 aaaaaggttt gcgaatatgt taaaatggtg aaaattaaat agaagtaggg gagctcttga
     2101 agggctgaga ggagagcgca agctttcgac ccgttgaacc tgatctggtt aatgccagcg
     2161 gagggatgga agtgcaaaca aaaagccacc taccagctgg caggtggctt tttttaatag
     2221 gggagaaaac gaaggtggtg aatagagcag ctaaaatttc ttttcgaaag acggcttatg
     2281 tcgctatttt ctgtgcttta gcggttgttt cttccggtgt tagtttcccc attggcccga
     2341 ctaaagtttt tccctttcaa cacgcaataa acgtggtagc cgggattatg atcggtcctt
     2401 ggtatggagg attagccgct tttttagccg gattgctgcg aattgcaatc gggactggaa
     2461 ctatctttgc cttgccgggg ggagttccgg gagccataat agttgggctt ttttacagat
     2521 ggttgcgtcg agattgggta gccttaaccg aacccttagg aaccggtttt atcggggctg
     2581 gattgagcgc gtatctggtt tcccctttta ccggaggaga ggcaactttt ttcttttttc
     2641 aatctgcttt tttagtaagt agtatccctg gatgtattat cggcctttta tttgttcggg
     2701 ttttgagaaa agctatatct gttgatacgt gggaggagat aggaggatag agatgtcaga
     2761 agatatccag cctgttccac agcaagagag aaacttatcg ggaatggatt tttttgttct
     2821 gtggatggga gcagccattg ccatatctga agtgtgggca ggaggaatgt tagctccttt
     2881 aggactcatc gggggcttgt gggctatcat actgggtcac gtaattggta acactcctct
     2941 ggctttaggg ggagtgttag gtagcgattc aggagttccg gctatggttt cagtgagagc
     3001 agcttttgga aaattgggct cttatctcgc ctcggctttg aacatcattc agttagcagg
     3061 ctggacggcg ataatgataa tcatctgtgc tcaatcagtt gataatatta ctcagatttt
     3121 agcgggatat actaatgtta agctctggat gcttattgcc gggattatta gcactttttg
     3181 ggctttagta ggaactcctc tttggaaatg gatgcaaaga atagcggttt tttctctggc
     3241 cattctctgc cttttcatga cttatagtgt tttgcataat gtgtctacag aggaattact
     3301 aaattggagg ggagatggct ctcttccctg gggggcaggt ttagacctcg tgatagctat
     3361 gcccatttcc tggcttcctt tggtttcaga ctactctcgt tttgctcgag agagccgctc
     3421 tgctttctgg gggacttggt ggggatattt tttagccagt tcctggatgt atgttttggg
     3481 cttattgctg gctctttcta ccggtcaggc taaccccata ccggtgatgt tagctctaaa
     3541 ttggggaatc attgctctgg tgattgtttt tttctctact tttaccacta cctttctgga
     3601 tatttactct ggagcgatgt cttttttaaa tcttttccct cgttttcagg agaaactaat
     3661 tactcttttc ttcggattag gaggcacgat tttagcttta gttttcccta tggatagata
     3721 cgaaagcttt ctgtatctca tcgggggaat ttttatccct ctttttgggg tggtaatttt
     3781 tgactatttc ttttaccgcc ggagaaagat tgagataaaa gaattatatg aggggaaagc
     3841 tctcgataga ggagctcttc tctcctgggt ggggggatta atgatatata ttatctttct
     3901 tcgtttttta cctcactggg gagcttcttt tcccagcttt ctcgcctcag gcttaattta
     3961 tctgggaaca aggaggaaat tcaatgactt atgaagaaaa atgcacactg atttttaaaa
     4021 agataaaaga agaaaagcct ctggttcatc atatcaccaa tttcgtggtc attaatgaca
     4081 atgctaatac tatgttggcc ataggagcac tgccggttat gagttatgcc cgggaggaag
     4141 tggaagaaat ggtagctctg tctcaagctc tggttctgaa tatcgggact ctaactccgg
     4201 aaacagtaga aagcgcaatt ttagcaggta ggaaagctaa cgagttaggt atcccggttg
     4261 ttttagaccc ggtaggggca ggagctactc aattccgcac tgagagtgct ttgaaaattt
     4321 tgcaagaggt taaaatctct attttgcggg gaaacacagg ggagatagga aatctactgg
     4381 gaataaaagc cgaagttaga ggagtggaag gaggagtagt agatgcagat acgagtgatt
     4441 tagcgcaaag agccagtcag aaattccaga ctgtcgtggc cataactgga aaaattgatg
     4501 tagtgagtga cggaaaaaga gtggtcaaga taaaaaatgg ggatattctc ctcaccagga
     4561 ttaccggaac tggttgcatg gccacttctc tttgcgctgc ttgcgcggca gtagagagag
     4621 attacctgtg ggcggcatct tcggctttag gttttctggg agtttgtgga gaaattgctg
     4681 ctcgtcactc tttagggccg ggtagctttc gagtttctct ttttgatacc ttttatggta
     4741 taaagaaaga ggaacttaaa gcgctgcttt ctctggaggt gaaagattga aaactataga
     4801 ttggtctctt tatgttatta ctgatagaaa aattcagaag aaaagtaatg aagaggtggt
     4861 agaggaagcc acagatgctg gagcttcagt ggttcaactg cgagaaaaag accttcctac
     4921 tcgtctatgg gtagaggaag ctcaaaaagt gagagaagtt actcaccggc gggggattcc
     4981 tttaatcatc aatgaccgta tagatattgc tttagcggta gatgctgatg gagtacactt
     5041 aggccaagaa gatatgcctt tagctttagc tcggaaaatc gctccttccc ttataattgg
     5101 ctattcggtc tccacggtag aagaagccct ggaagcagaa agagaagggg cagattattt
     5161 aggagcgggg acggttttcc ccacttctac taaaaaagat gccggggaac ccattggttt
     5221 agaaagactg agagaaatta aaaaagcagt taatataccg gtggtggcca tcggggggat
     5281 aaacctttct aatctggagg aagttttagc aaccggtgtg gatggggtgg cagtggtatc
     5341 ggctattgta ggcgcagagt cggtcaagga agccactcgt tctttccggc aaaaaattga
     5401 gctttttcgg agaagaccat gaaactcaaa gagtggggag agtttgggat tattgactat
     5461 ttgaaaaatt ttttcccttc ccgggggaaa agagtggttt taggaattga agatgactgt
     5521 gcggtggtta gaggccctcg aggttttttc tatcttctca cctgtgattc ccaggtagaa
     5581 aatgtacatt ttattttatc tcttttttct cccttttctc tggggaaaag agttctggca
     5641 gtgaatttaa gtgatattgc cgctatgggg ggaaaacctc tttttgctct tctttcctgg
     5701 gtgttgaggc cagatatgga agcagagtgg ctaaaggaat ttatgcgggg aatcaaggaa
     5761 gaagcggaag ctcacggagt agacattata ggagggaatt tatctcgtac tgaaagtcag
     5821 atggtttttg acttaacttt agtgggagag gtagaaaatg accgcccttt gcttttgcgc
     5881 cgtaatgctc ggcctggaga tttagtttta gttacgggaa ctttgggtga ctcacgagca
     5941 ggtttagaga tagctcttcg gggtagcgag gaagaaaaag aaaagtattc ttttcttttg
     6001 cagcgtttct ttactccttc tcctcgcctg caagtaggga ggttattgca cagtttgggg
     6061 gagagagtag ctacgattga tgtttctgat gggttttctc aggatttagg gcatattgtg
     6121 gaggagagtg aagtgggggc agtggtagag gtggataaac tacctatttc tccttctctt
     6181 cgggagtgga gtgaagagga aaacaaagac cctttccttt ttgctctttc gggaggagaa
     6241 gattttgaat taatttttac cgttccccgg gagcgcggaa aagaagttaa agaattcata
     6301 gaaatagaaa gtggagttcc ggtaactatt gtgggagagg ttgtctcgca aggaggggtt
     6361 tctctgcgta gagatggaag agaagaaaaa gaagtcgcct cgggctggaa tcattttcaa
     6421 tagaggagga aaaaagatga aaagagcgct aaccatagct ggttcagatt cgggaggggg
     6481 agcaggaatt caggctgacc tgaaaacttt ctgtgctttt ggagtgtacg gcatgtcatc
     6541 tattacctcc ataaccgccc agaatactca aggggtagaa gccatttttg acttacctcc
     6601 ctctctggtg gccaaacaaa tagagtgtgt ggcaagtgat ataggagtgg atgcggctaa
     6661 aaccggtatg ctttctaatt cttctatcat tgaggcagtg gctgaggaga ttaaaaagtt
     6721 taatatagaa aaattggtgg tagacccggt gatggtatct aaaagtggag ctttgcttct
     6781 tgagcgagaa gccgtagatg ctcttgtgca caagcttttc cctctctctt ttttgattac
     6841 tcccaatctt cctgaagctt cttttttagt gggggaggag ataaaggata tttccgatat
     6901 ggaaaaggcc tgtatatcgc tcaaaaagtg gggtcctcgc tatgttctcc taaaaggagg
     6961 gcatctcccg gaagaggaga tagtggatat cctttttgac ggagaagaat ttatttcttt
     7021 ccccgctcga cgtatcgcca ccaaaaacac ccatgggaca ggttgcactt attctgcggc
     7081 tatatgtgct ctcctatctt taggatactc aattaccgaa gcagtaaaaa cggctcacga
     7141 atatatgatt caggtgatta cccactcctt aaacctgggt aaagggtttg gtccggtcaa
     7201 ccatttagct cctctttttc ttagagaggg ggtgaatccg gaagagggag ggaaatactg
     7261 gtgaaagagt cccggatagg gacttctggt tggtcttatc ctcattggga gggagtgttt
     7321 tatcctactc atttgcctca aaataagtgg ctttcctact attctcagtt ttttgatacg
     7381 gtggaggtta actcttcttt ttatcatctc cctcgttcca ctactttttc caaatggaga
     7441 gataccactc ctccggagtt tgttttttcc gttaaagcca gccgctacat tacccatata
     7501 aagaaaatga gagaggtaga agaacctctg gcaaagcttt atgaggcggt aagagagctg
     7561 gggaacaaat taggtcctct tctttttcaa tttcctcctc aacttaaatt taatagagaa
     7621 gttttttcct cttttgtgga gcttctttct cctgattttc gttatgtttt tgagtttcgc
     7681 aatgagagtt tctttaccgg agaagtttat gaaattctgg aaaagaaaaa tatctgtctc
     7741 tgtttttccg ataccccttc ttttccctat caagaagtta taacttcttc ttttatctac
     7801 cttcgtcttc acgggcacgg cagcctttat accgattttt acagtgaaga agaactcgaa
     7861 ggatggggag ctaaaattcg tttctggctc ggggagcggg atgtttattg ctactttgat
     7921 aatgattacg aaggctttgc agtgaagaac gctcagcgcc tcagagaaat tttatcttga
     7981 gaaggtatag aattcttact tttgtgagat aatttgaggg ggtgagaagt atggaaaaaa
     8041 ggaaattagg aagtactggc ctttctattt ctcttctggg gttaggcggt tttcatctgg
     8101 tagagctacc agagaaagaa gccctgcgga ttgtcaattt gtatttggat cagggaggaa
     8161 attacttaga aaccgcagag agctatgggt ctggcaattc agaggaaaag ataggtaaag
     8221 tggccaaagt tcgaagaaat gacttcatcc tggctactaa atctgcacaa aggagtaaag
     8281 aaggtataac cagagcatta gagggaagcc tgaggagact caacaccagt gaagtggata
     8341 tatttttcat tcatggctta aaccgttttg aagagctgga tgaaatatcc tcttcccagg
     8401 gtgctttaga gggagtagaa aaagctaaaa aagaagggaa aattcgtttt gtagcttttt
     8461 ctaatcatgt tactcctgaa gtagcactca atgctctttc tctttatcct tttgacattt
     8521 tgatgattcc cttgaattat tttgaccgat tcaattttcc tggctgggaa gaagaagttt
     8581 taccccgggc tcgagaaaaa agaatgggga ttctggctat gaaagcgctg gctgacggtt
     8641 ttttgtggga aagtgaacga gaggctatac gttacaccct gagtctcggg gtagatagtt
     8701 tagttatggg agctaacaca gaagagtatc taagaaggga tatagaagtg gtggaaaact
     8761 tcgtgccttt cactccggaa gaaatgaaaa aagtccggga ggaagctccg gagctgggta
     8821 gctatgtttg ccgccagtgt gataaatgtt taccttgccc tttgggcatt gatatccccg
     8881 gagtttttct tctggagggg caatatgacc gacagatgca agatgggata attcgtgatc
     8941 ctgctcaata tgccctgcga gaccgcctcc gtttctggct ggggaatcaa gattacgccc
     9001 agaaagctta tcaggaattg aaccctaagg cttcggtgtg caacgaatgc ggggagtgcg
     9061 agcctcgctg tccctataaa atacccatca tttataaatt gaatttagct catgaaaagc
     9121 taactgacac tcgtcctccc ggctactgtc gcatatcgta gtcgtgagac tttatattat
     9181 ttcgcaagac ctgttataat gacgccgggg cgaatcgctg cttcttttgg gagagggaaa
     9241 gagtgaaata actatagggg ggcggcgtcc ccctatagct ccctttcttt tttgttatcg
     9301 caatgactct ccttattcgt caccccggca atttttaagc cggggtccat attatttaag
     9361 ggcaaaaacg tggattcccg ataaaaactt tcgggaatga caaaaaaggg atgtcacccc
     9421 tgaatgtttc tatcaggggt ccatggttta aaaaagtatt tagattctcg actaaatttc
     9481 gggaataatg gtgcgagaca cgcctatggt tcacaatgaa agg
//
LOCUS       DAOPSS010000058         9486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6048_length_9486_cov_7.900965, whole genome shotgun sequence.
ACCESSION   DAOPSS010000058 DAOPSS010000000
VERSION     DAOPSS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6048_length_9486_cov_7.900965"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1554
                     /locus_tag="PKN31_04810"
     CDS             124..1554
                     /locus_tag="PKN31_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOA99278.1"
                     /translation="MASSKDNAQDISFFLEAKTPADVRKAIFKLALPAMLEFMLMMLV
                     QMIDMIQVGSLGPWAIAATGLAAQPTMLIFSVFSALVVGTTALVARAAGAGDREYASR
                     VTRQSFVVVLLMGVIVTAVGIPASGAIIRLMGADVDTIGPGTIYMKIIMAGTVFTVGN
                     MVLAASLRGVGDMVTPMISNIVANVVNIFMNWLLINGNLGFPRLEVAGAAIATSFSRL
                     IAFCITMHAVYARDKYISISIHDSYRFEPDVIRSVLNIGTPAAIEQLIMRTGALLFAR
                     LIAGFGTIMFAAHQIAINIDGLGIVPAMAFQMAATTLVGQGIGAQKPELSEEATRQSI
                     IVSTIIMSLIGVAYFFLGKYAIMIFTRDAQVISLGESALKILAFSQPFTAVYFVVAGA
                     LRGAGDTKYAMYASAIGMWVTRLGLGYILADLLNMALIGAWIAVTVDMAVRMVLVLKR
                     FSSGVFEEYSREYGKKYSEESLSESI"
     gene            1723..3048
                     /locus_tag="PKN31_04815"
     CDS             1723..3048
                     /locus_tag="PKN31_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA99279.1"
                     /translation="MSKKILLVNPWIYDFTAFDLWLKPLGLLYLASHLLNQGYEVELL
                     DCLNRLDPEFPPVSRGPRYLREAGCGSYYKEILPKPSFFQDVPRYWGRFGLPFDKVKS
                     RMEKLSPPDFVFITSGMTYWYPGAVVAKELARKIWPEAKTVLGGIYPALCPEHAEGWG
                     FDYVVKATDPLSVMKELECILETDFKVESYSDYFSLRPAFFLYPELSYIVLLTSLGCP
                     FRCTYCASGKLYPSFWCRPPEDIIEEILFSREKYGIKDIAFYDDALLFQGEKHFLPLL
                     KKLEELSLDLRFHLPNSIHPLYVTPQIAQLMKKVGFKTLRMSLETSSSLWQEKTGGKV
                     NNYAFEKATRYFLDAGFAPQEIEVYLLFGLPGIPEEEMVNSIEYVKSLGLKPRLSLYS
                     PIPDTPDYYKIEGLLPEDEPLFHNKIAFLYKSGNALLYQKLQRAIAPED"
     gene            complement(3045..3953)
                     /gene="nadA"
                     /locus_tag="PKN31_04820"
     CDS             complement(3045..3953)
                     /gene="nadA"
                     /locus_tag="PKN31_04820"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOA99280.1"
                     /translation="MRDWKGEIEKLKKERNAVILAHNYQLPEVQDIADFVGDSLGLSI
                     EASKTDAQVIVFCGVFFMAETAKILSPQKVVVIPDQEAGCPMADMITAPQLVDLKNRH
                     PEAAVLCYVNTKAEVKAECDFCCTSANATKVVEKGLNSASSIIFVPDQYLASYVSRKT
                     GREFIVWPGYCPIHAAISGEDVERARELHPQAEVLAHPECRPEVTEKADQVLSTEGMC
                     RYVKSSPADEFIIGTEVGILHRLQKENPTKHFYPVKEEAVCPDMKKINLEKVAFALQE
                     MKYQVELPPSIIQKAALSIERMLQFT"
     gene            complement(3979..5016)
                     /gene="mnmA"
                     /locus_tag="PKN31_04825"
     CDS             complement(3979..5016)
                     /gene="mnmA"
                     /locus_tag="PKN31_04825"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOA99281.1"
                     /translation="MRKKVLVAMSGGVDSSLSCVLLLERGFEVAGVTMHLAKGEEESD
                     TAIKGAREICRALSIPHYVVDFSREFEELVVEDFVSQYLKGKTPNPCVVCNRRLKFGK
                     LLEYARHKGFDFLATGHYAKIEEKEGKYFLRRPKDRKKDQTYFLYDIGRDNLPYIIFP
                     LSDFTKKEVKELSLKYNLHIAEESESKDICFLPEGDYRKFLEERISLDSPGFIVTTEG
                     KIVGEHKGYFRYTIGQRKGLGVAHSRPLYVVAVHPERNEVVVGEREALEARGLVASHF
                     QWLVDELPSSVFAQVRYLQKEKACSVEIVGPDEIRVIFKEKVEMISPGQSVVLYSDDT
                     VLGGGIIEEAF"
     gene            complement(5022..5933)
                     /gene="cysK"
                     /locus_tag="PKN31_04830"
     CDS             complement(5022..5933)
                     /gene="cysK"
                     /locus_tag="PKN31_04830"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOA99282.1"
                     /translation="MRIKDNITQLVGNTPLVRLGKIGKGLPGELVAKLEFFNPCGSVK
                     DRIAVAMIEEAEKQGFLKTGSLVVEPTSGNTGIGLAFVCAERGYRLLLTMPESVSVER
                     RKILTRLGAEVVLTPASRGMQGAIERAQKIVEDTPGAFMPQQFENPANPRAHRETTAR
                     EIWEDTDGEVDIVVAGVGSGGTITGIAQALKEKKPAFQAVAVEPLRSPVLSGGSPGQH
                     SIQGIGAGFIPPVLKLELLDEVIAVSDEDAFSMSERLAKEEGILVGISSGAALWGAVQ
                     VATREENKGKLIVVIIPDTGERYLSLW"
     gene            complement(5944..6915)
                     /locus_tag="PKN31_04835"
     CDS             complement(5944..6915)
                     /locus_tag="PKN31_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOA99283.1"
                     /translation="MRAIGLVSGGLDSALAIFLLKKQGIEVIGLSFLSPFWESKNTLK
                     RLAQELEIELREIEVGEEYLELMKNPQFGYGKNLNPCIDCKIWMLKEARREMEKEGAS
                     FVFTGEVVGQRPKSQLKNTLRLIEKQSGLEGYLLRPLSAKLLPPTIPEEKGWVKREEL
                     LDLQGRGRKRQLELAEKWGIKHFSPPAGGCLLTDPNFSRRLKDLWESEEAYNFESVEL
                     LKVGRHFRLRPSFKLVVGRNQIENEKLKEFVREDDILFFPREGKGPLALGRGEADEPL
                     LQEAASLLARYIHFPGEVVSVGAKTGSLLRVLEVERMSEEEVKKRMI"
     gene            complement(6924..7373)
                     /locus_tag="PKN31_04840"
     CDS             complement(6924..7373)
                     /locus_tag="PKN31_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOA99284.1"
                     /translation="MKIRFSTRSRYGLRFMIELATYYGKKDLVFLREIAEKEGISEKY
                     LTQLAIPLKARGLISSRRGAGGGYRLSRHPAQITAKEVVEALEGGIFPVDCLESPDIC
                     SRTSFCAARKMWKELQDKISETLEKFTLENLAQMLTNSPPSPIDFSI"
     gene            7502..8587
                     /locus_tag="PKN31_04845"
     CDS             7502..8587
                     /locus_tag="PKN31_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA99285.1"
                     /translation="MTSYERIINALEGKPTDILPYIDGFNCLEARLSFFGPQVMKAGW
                     EEIALLEAELFQSDWVIIPAPLNIPGGPGIFCDIISEDDTHLLARTFYGGIWYWRKKP
                     YHAKAIYNPVNNLEDWERLSEPDEEKLRKRIRTLREPVKKLKDRGYFVTMEGKGAFEA
                     TWMLFRGFENTLMDIVLNPERVKKMSERAVNAIIKLGLMVKEECEVDGIWITDDLGAQ
                     KAPYFSPQQYRQIFKENHRKMVEAFKKAGLKVTFHSHGNIMPLFQDLVEVGFDSIDPL
                     DSYDGMDFPALKKEFGEVITLKGGISCTIGQMNKEELKEHIGEAVKIGGDRRFILSGA
                     GGVPPEMTLENFNHYRELIYKARRSEL"
BASE COUNT         2537 a         2302 c         2026 g         2621 t
ORIGIN      
        1 aaaaagtgcc caattgcgat tgataaaaaa tgtcaattat tgtatgatgg tagaatgtag
       61 agtttctaac cgggtctgcc cctgtcagga aatccaacat ttttagaagt aaagggtgat
      121 tttatggcat cgtcaaaaga taacgctcaa gacatatcgt tttttttaga agcgaaaacg
      181 cctgctgacg tacgtaaggc aatattcaaa ctggctttgc cggcaatgct tgaatttatg
      241 cttatgatgc tggtgcaaat gatagatatg atacaggtag gttccctggg cccctgggct
      301 attgctgcaa ccggactggc agctcaacca accatgctga ttttttcagt gtttagtgcg
      361 ctggttgtcg ggactacagc ccttgtcgcg agggctgcgg gagctggaga tcgtgaatat
      421 gcctctcgtg taaccagaca gtcttttgta gtggtgttac ttatgggagt tattgtaacc
      481 gcagtaggta ttcccgcttc tggagctatc atccgattaa tgggtgcaga cgttgataca
      541 attggcccgg gaacaattta tatgaaaatt ataatggcag gaacagtgtt tactgtgggt
      601 aacatggtgc tggcagcctc tttaaggggc gtaggcgata tggtaacccc catgatttca
      661 aatatcgtag caaatgtcgt caacatattc atgaactggt tgttaattaa tggcaacctt
      721 ggattccctc gattggaagt ggcaggcgca gcaatagcca catccttttc caggttaata
      781 gcattttgca ttactatgca tgcggtatat gcccgggata aatatatcag catatccatt
      841 catgattcat atcgctttga gcccgatgta atacgaagcg tgttgaatat tggcaccccg
      901 gcagcgatag agcagctgat tatgaggact ggtgcattat tgtttgcacg actgattgcc
      961 ggatttggca ctataatgtt tgctgcccat cagatagcga taaacattga tggtctggga
     1021 attgtaccgg caatggcgtt tcagatggcc gccaccactc ttgtagggca gggtataggc
     1081 gcacaaaaac ccgaactttc agaagaagca acacgacaat caataattgt cagtacgata
     1141 atcatgtcgt taattggcgt tgcttacttc ttcttgggta agtatgccat aatgattttt
     1201 actcgagatg ctcaagtaat ctccttggga gaaagcgcct tgaaaatttt agcattttcc
     1261 cagcctttca ctgctgttta ctttgttgtt gctggtgccc tgcggggtgc aggggataca
     1321 aaatatgcca tgtatgccag cgcaattggc atgtgggtca cccgcttagg cctgggatac
     1381 atattagcag atttattgaa tatggcactt attggtgcct ggattgccgt taccgtagat
     1441 atggcggtga gaatggtgct ggtgcttaaa agattttcaa gtggtgtatt tgaggagtat
     1501 agcagagaat atggcaagaa gtatagtgag gagagcttaa gcgaaagcat atagtataga
     1561 aaatagcggg cgacaaaaaa ggcaatagag acattcaggg gtgacgagca aggtggcttt
     1621 tcctccgcgt tgttttggtc tttttctctt gtcctttttg tttttctctt tttggaacca
     1681 tgagagggca aagccctctc atggttctgt tataatcttc ttatgagtaa aaaaatactt
     1741 ttagttaacc cctggattta tgactttact gctttcgacc tctggctcaa gcctttaggg
     1801 cttctttatc ttgcctctca tcttctaaac caggggtatg aagtggagct cttagattgc
     1861 ctgaaccgtt tagatcccga gtttccacct gtctctcgtg gtcctcgcta tctgcgagaa
     1921 gcaggctgcg gtagctatta taaagaaatt cttcccaagc cctctttttt tcaggatgtc
     1981 cctcgctact ggggaaggtt tggcttgcct ttcgataaag tgaaatccag aatggagaaa
     2041 ctatcccctc ccgattttgt ctttatcacc tcaggcatga cttactggta tccaggagcg
     2101 gtagtagcga aagagctggc caggaaaatc tggccagagg ctaaaacggt tttaggagga
     2161 atttatcccg ctctctgtcc tgagcacgcc gaaggatggg ggtttgatta cgtggtgaaa
     2221 gctaccgacc ccctttcggt gatgaaggaa ctggaatgca ttttagaaac agatttcaaa
     2281 gtggaaagct atagtgatta tttttccctg agacctgctt tcttccttta cccggagctc
     2341 tcttatatcg tccttctcac ttctttagga tgcccctttc gctgcaccta ttgtgcttca
     2401 ggaaaactct atccttcttt ctggtgccgc ccccccgaag acataataga ggaaatcctg
     2461 ttttccagag aaaaatatgg aataaaagac atagccttct atgacgatgc tcttcttttc
     2521 cagggagaga aacactttct tcccctctta aagaagttag aagagctatc tttagacctt
     2581 cgctttcacc ttcctaactc tattcatccc ctgtatgtta ctccccagat tgcccaactg
     2641 atgaagaaag tggggttcaa gactttgcgg atgagtttag aaacctcttc ttctctgtgg
     2701 caggaaaaaa caggggggaa ggtaaataac tatgcttttg agaaagccac gagatacttt
     2761 ttagacgctg gttttgctcc tcaggagata gaggtatacc tcctctttgg cctgccgggc
     2821 atccccgaag aagagatggt aaattccatc gaatatgtaa aaagcctggg gctaaaacct
     2881 cgcctgtctc tttattctcc tatccccgac actcccgatt attataaaat tgaggggcta
     2941 ctgcccgagg atgaacctct cttccacaat aaaatcgctt tcctctacaa aagcggcaat
     3001 gcccttctct atcaaaaact gcagagagca atagccccgg aagattaggt gaactgaagc
     3061 attctctcga tgctcagcgc tgctttttga ataatcgacg gaggaagctc cacctggtat
     3121 ttcatttctt ggagagcaaa agccactttt tccaggttta tttttttcat atccgggcac
     3181 actgcctctt ccttcaccgg ataaaaatgc tttgtcgggt tttccttttg caggcgatgg
     3241 agaataccca cttctgttcc aatgataaac tcatcagcag gggagctctt cacatagcga
     3301 cacatacctt cggtagaaag cacctgatcc gccttctctg ttacttccgg gcgacattca
     3361 ggatgggcaa gtacctccgc ttgaggatgg agctctctgg ctctttccac atcttctccg
     3421 cttattgccg catgtatcgg acaatatcct ggccagacga tgaattccct tcccgttttt
     3481 cgagagacat aactggccag atactggtcg ggaacaaaaa taatggaaga agcagagttc
     3541 agtccctttt ccaccacttt agtggcattt gctgatgtac aacaaaaatc acattctgcc
     3601 tttacctccg cttttgtgtt cacatagcaa agcaccgctg cctcaggatg gcgatttttg
     3661 aggtcaacaa gctggggagc agtaatcata tccgccatgg ggcatcctgc ctcctggtcg
     3721 gggattacca ctactttttg aggagagagg atttttgccg tctccgccat gaaaaaaact
     3781 ccacaaaata cgataacttg agcatcggtt ttgctcgcct ctatgctcaa tcccagtgaa
     3841 tctcctacaa agtcagctat gtcttgcact tcaggaagct gataattgtg ggctaaaatt
     3901 accgcattcc tctccttttt tagtttttcg atttcccctt tccagtctct catcaatttt
     3961 cacccctaat cgaaaatctt aaaaagcttc ttcaataatc cccccaccca gaactgtatc
     4021 gtcactatac aaaactaccg attggccagg actaatcatc tctacttttt ctttgaatat
     4081 aaccctaatc tcatccgggc cgacaatctc taccgagcag gccttttctt tctggagata
     4141 gcgcacctga gcaaacacag acgagggaag ctcatcaacc aaccactgga aatgagaagc
     4201 caccaacccc cgagcctcga gcgcctctct ttcccctacc accacttcgt ttctctccgg
     4261 atgcactgcc accacataaa gaggtcgaga gtgagctact cctaagccct ttctttgacc
     4321 aatggtatag cgaaaatatc ccttgtgttc acccactatt ttaccttctg tggtaactat
     4381 aaaaccgggg gaatccagag agattctctc ctccaggaat ttccggtaat cgccctcggg
     4441 gagaaaacaa atgtctttac tttccgattc ttctgctata tgaagattat atttcaaaga
     4501 gagctccttc acctcttttt tggtaaaatc ggaaagagga aagatgatat agggaaggtt
     4561 gtctcttcca atatcataga gaaaataagt ctggtctttc ttccggtctt tgggtcttct
     4621 taaaaaatat tttccttctt tttcttcgat tttagcataa tggccagtgg ccagaaaatc
     4681 aaatccttta tggcgggcat actccaggag cttgccaaat ttgaggcgcc ggttacatac
     4741 cacacagggg ttgggagtct tgccttttaa gtattgagag acaaaatctt caacgactaa
     4801 ctcctcaaac tcccgggaaa aatccaccac ataatgggga atagaaagag cgcggcaaat
     4861 ctcccgggca cctttaatgg ccgtatcaga ctcctcctct cccttcgcaa gatgcatggt
     4921 taccccggca acttcaaacc ctctctctaa aagcaaaacg caggatagcg aagaatctac
     4981 tcctccgctc atagccacca gtactttttt tctcacgttg ctcaccaaag agataaataa
     5041 cgctctccgg tatcaggaat aattactaca atgagcttcc ccttgttttc ctctcgagtg
     5101 gccacctgca ctgctcccca cagagccgcc ccggaggaga tacccaccag tattccctcc
     5161 tcttttgcca gacgctcgga catcgaaaaa gcatcctcgt cgctaacagc aattacctca
     5221 tccaggagct ccagctttaa aaccggagga atgaatcccg ctccaattcc ctgaatggag
     5281 tgctgccccg gagagcctcc tgagagaacc ggagagcgaa gaggctccac tgccactgcc
     5341 tggaaagctg gtttcttctc cttaagagcc tgagcaatcc cggtgatggt tccgccactt
     5401 cccactcctg caaccacaat atccacctca ccatcggtat cttcccatat ctctcgagca
     5461 gtggtttccc gatgagcgcg agggttagcc ggattttcaa actgctgagg cataaaagct
     5521 cccggggtgt cttctacaat tttttgggct ctctctatcg ctccttgcat gcctcgagag
     5581 gcgggagtga gaaccacttc ggctcccagg cgagtcaaga ttttccgtct ctctaccgag
     5641 acactttcgg gcatagtcaa aaggagacga tagcctctct cggcacacac aaaggctaaa
     5701 ccaataccgg tattgccgct cgtgggttct accaccaaag acccggtttt gaggaagccc
     5761 tgcttttccg cttcttctat catcgccacg gcgattcggt ctttgacgct cccgcagggg
     5821 ttgaaaaatt ctaattttgc caccagttcc cctggcaagc ccttcccgat ttttcccagc
     5881 ctcactaaag gggtgtttcc caccagctgg gtaatgttat cttttattct cataaatcac
     5941 ctcttaaatc atccgcttct ttacttcttc ttcactcatt ctctctacct ccaacaccct
     6001 caaaagtgag ccagtttttg cacccacaga aaccacctct ccggggaagt gaatatagcg
     6061 ggcaagaagg gaggctgcct cttgaagaag aggctcatca gcttctcccc gaccgagagc
     6121 caggggaccc ttaccctccc gggggaaaaa caatatgtca tcttcccgga caaattcttt
     6181 cagcttctca ttctctatct ggtttctccc caccacaagc ttaaaagaag gacggagacg
     6241 aaaatgtctt cctactttca agagctccac gctctcaaaa ttataagcct cttcgctctc
     6301 ccacaggtct tttaacctcc gggagaaatt ggggtcagtt aaaagacatc cccctgccgg
     6361 cggagaaaag tgttttatcc cccacttttc cgctaattcc agttgccttt ttcttcctct
     6421 cccctgtaaa tcaagaagct cctctctttt tacccagcct ttttcttcag gaatggtagg
     6481 aggaaggagc tttgccgaca aaggacgcaa aaggtagccc tccagcccac tttgcttttc
     6541 tataagacgc aaggtatttt ttagctggga tttgggacgt tgtcccacaa cttcaccggt
     6601 aaaaacaaaa gatgctccct ctttttccat ctctcttctt gcctctttga gcatccagat
     6661 tttgcaatca atgcagggat tcaggttttt tccataccca aattgaggat tcttcatgag
     6721 ctcaagatac tcttctccta cctctatttc ccgaagctct atctccagct cctgagctag
     6781 ccgcttcaat gtatttttac tttcccagaa agggctcaaa aaactgagcc cgatcacctc
     6841 tatcccctgt tttttgagca gaaaaatagc taaggcacta tccagaccac ctgacacaag
     6901 accaattgcc ctcatcagct ctcctaaata gagaaatcta tcggtgaagg agggctattg
     6961 gtgagcatct gagctaaatt ttccagagta aacttttcta aagtctcaga aattttatct
     7021 tgaagctctt tccacatttt ccttgctgca cagaaagaag tccgggaaca gatatcggga
     7081 ctttccagac aatccacggg gaaaattcct ccctctaaag cttctaccac ctcttttgcc
     7141 gtaatctgag cggggtggcg agagagacga tagcctcctc ctgctcctcg ccgagaggaa
     7201 attaaacctc gagccttaag aggaatagcc agttgggtca ggtatttttc tgaaataccc
     7261 tctttttctg ctatctccct gagaaagacc aggtcttttt taccgtaata agtggccagc
     7321 tctatcataa accttaagcc atagcgggaa cgagtagaaa aacggatttt catcactccc
     7381 aaattttata atcctgattg ccttaataat attatagaaa cgaaaatcct ggctgtcaaa
     7441 ctcgaaaacg ttgcaaatga taatataata aaagaaataa aagttaaagg ggaaaggctc
     7501 tatgacttct tatgagagaa taatcaatgc tttagaagga aagcccaccg acattcttcc
     7561 ctatatcgat ggttttaact gtttggaggc tcggctatct ttctttggcc ctcaggtgat
     7621 gaaagcaggg tgggaagaga tagctcttct cgaggctgaa cttttccagt ctgactgggt
     7681 aataatccct gctcctttga atataccagg tggccccggt atcttttgtg acataatttc
     7741 cgaagacgat acccacctcc tggctcgcac tttctatggg ggaatctggt actggcgcaa
     7801 aaagccctat cacgccaaag ccatttataa cccggtaaat aatctggaag actgggaaag
     7861 attgtccgag cccgatgaag aaaagctgag aaaaaggata agaaccctcc gagaaccagt
     7921 gaaaaagctc aaagaccgag gatattttgt cactatggag ggcaaaggag cttttgaggc
     7981 tacctggatg ctctttcggg gatttgaaaa caccttaatg gatattgtcc tgaatcccga
     8041 gagagtgaag aaaatgtcag agcgagcagt aaatgctatc atcaaactgg gtttgatggt
     8101 aaaagaagag tgtgaagtag atggtatctg gataaccgat gatctcgggg cacaaaaggc
     8161 tccctatttt agccctcaac aatatcgaca gattttcaag gaaaatcacc gaaaaatggt
     8221 agaagccttt aaaaaagcag gtctgaaagt taccttccac tcccacggta atattatgcc
     8281 cctgtttcaa gacctggtag aagtgggatt tgattccatc gatcctctcg attcatatga
     8341 tggaatggac tttccagccc taaaaaaaga attcggagag gtgataactt taaaaggagg
     8401 aataagttgc accattggcc agatgaacaa agaagagctg aaagagcaca tcggagaagc
     8461 ggtaaagata ggaggagaca gaagattcat actctcagga gcaggaggag ttcccccgga
     8521 gatgactctg gaaaacttta accattatcg agaactcatc tacaaagctc gtcggagtga
     8581 actatgaaag aagaggcggc aatcacaaga gccgggtttc tcaccgagaa tcgctcaaga
     8641 gcatggtgtg ttcctcctcc cgggtatagc gagaagcgat tttacctatc ccgtagctat
     8701 tctacaataa tgtcccttgg aaaattctca agaagcaccc ctttggtaaa gcaaaacgaa
     8761 gtgatgatgg gatgacctgc agcgctaaaa acaaaaagac aattcaaaag agaagccagg
     8821 tcgtgggttt ttggcgctct tatgtccaga taagccgtag gctttgagat gcccctctac
     8881 tgcttgctgg cagtgaaaac aaacaatgcc catggggggg ttcggccacc tccagcagat
     8941 actcagccga cataaagtct tcatcggctt tcttgattca gcagctgatg atttcctcct
     9001 tcatacctct attccctcaa gatagacttc gtgaacaatg gcagcttttc cttttcaaaa
     9061 actgcagaat tctttataag aatatcaaaa gagtagccgg gaaaagaaag gtggaggtca
     9121 ttataaagaa cgctccagct tcctttcctt tttgggagag gaagctctgt atcgatgaga
     9181 accagcaaat cccagtcact gctcttttga ggatcccccc acgctcggga accaaaaaga
     9241 attactctca ccttttcgat gccctctcga gaaagagcgt ccacaatcac atttttacat
     9301 ttttaatggc ttttgcttcc tctaacattt aatacacgct ccagaatttt ttccgaatat
     9361 attcgctaac tataagcata tcaccaaaca acctgtgaca gaacaataag agacgctctc
     9421 ttcttcgggg gttgtatttg gcaatatgaa tttgcagcat tccggaagtt aatttttgca
     9481 gcacct
//
LOCUS       DAOPSS010000059         9369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6156_length_9369_cov_9.661048, whole genome shotgun sequence.
ACCESSION   DAOPSS010000059 DAOPSS010000000
VERSION     DAOPSS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6156_length_9369_cov_9.661048"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1153
                     /locus_tag="PKN31_04850"
     CDS             92..1153
                     /locus_tag="PKN31_04850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOA99286.1"
                     /translation="MKVLHTADIHLREYEDERWKALQKLIEIGKAEKVELFVISGDLF
                     DRGIDAETLRPKIREIFSNTGFKIVLIPGNHDDESYKSGMYFGEDTIILNDLNEPFEY
                     KDVRIWGLPFEPLEGEKVVEKLRSIANNLTSDRKNILLYHGELLDAFFSRRDFGEEGE
                     ERYMPVKLSYFKDLNLDYILAGHFHSRFDMWKLENGGYFVYPGSPVSITKRETGRRKV
                     NIFEIGQPPREHPLDTFHFEEVVIELDPFMGENPVEIVKERLKASHPEASIILTVRGY
                     INSEEIEMSESELVEQIKEVIKDKPVEEYLEFRDMYTILEDDLFKSFMSKLAETPFTE
                     EKKKELLDLTIQAMMEARL"
     gene            1150..3483
                     /locus_tag="PKN31_04855"
     CDS             1150..3483
                     /locus_tag="PKN31_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99287.1"
                     /translation="MRVKEFSIMRYGPLSNTGRILLHSFNLFWGKNEDGKTLTIDALV
                     KMLLGRSVREFEHINRVEENPEGYVIVESEKGEEIKLPERGDLTKIAGLTSSECCNIF
                     IVRNSNLSIARDAAQESKFYIDVTDRLTGLRTGEIRRIKEALKEMGKITPQGAFRDIR
                     DEKLKTRIEDAQNLIEKIKSLCKETEEKRFDELEKESAELKEEIERIDREMKAFEDAR
                     KREKYERGREALDNFKSSLERLKDLEVYNEEDERLWRDCHRDIMTLEEERKKVAEELE
                     ESKEEFKTINEKLDEAERDFRVLSERKKNLDEEIKPELRAYENKSVELVEQQEKDKVF
                     TLLGIIFAVLLGISLLGVIFSPSPLFYALAILSSVLVLVSVVFQLRLVREKARFAKIL
                     EENKLSLSKLGIEAESVEKILFNIQRFDEGYHRKFEELQDIRRKRENLEEKIENLQSK
                     KIPEIENKLKSLEEKIEEIKRKSKEESLEEYTEKLKLKQKLERLLGEQRSILKSHLGE
                     KGKSLEENISYWSEEIEKLEIYEDKAKNVKYSETAVVDLGKKKAELEEKLKDINEIMK
                     AIRKDLDDIERKVNEILRLEEEYLHCKTLVDLEAVKDELQKFVDECENNRSNVLEVIR
                     IFEEIEAEEKEKVSELFGRGSSTSKYFGEITDGLYEEVVFNREEERIKVRRKDNMMLE
                     AEKLSGGAYDQLYFSIRLALGERLLENKKGFFIMDDPFIKADPDRLRRQIETLKRITE
                     LGWQVMYFSAKGEIKEALREDIDCGNINYVELPGILA"
     gene            4690..5292
                     /locus_tag="PKN31_04860"
     CDS             4690..5292
                     /locus_tag="PKN31_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOA99288.1"
                     /translation="MKKKFDRVYQFKITLKGIRPPIWRRIHVPETYTFWDLHVAIQDA
                     MGWEDAHLHEFEMVSPSTGEKVRIGTPTEEAEVFGQQIIPEEKQKIANWFSMENRVAS
                     YVYDFGDDWEHKIELEKILPREKNIKYPVCIAGKRACPPEDCGGVRGYEEFLEAIRNP
                     EHEEHSIMLEWIGGEFDPEYFNPEEVIFEDPDERRKYLWE"
     gene            5367..5720
                     /locus_tag="PKN31_04865"
     CDS             5367..5720
                     /locus_tag="PKN31_04865"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOA99289.1"
                     /translation="MRDFIKEAEKLLIAARHDLNMGDYYSCFSKSYHAMVLSIKASLS
                     LFEGRFSKTEIEMLNRDVEKKFKDIHDQILLMDNGAGFILNEKEVKEFLEEAGDFVQK
                     SKNDLEEWLQTKGEK"
     gene            5797..6300
                     /locus_tag="PKN31_04870"
     CDS             5797..6300
                     /locus_tag="PKN31_04870"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOA99290.1"
                     /translation="MRESTRTLTYSAIAIALVAIATMSLQIPIPQTRGYINLGDTLIF
                     VFALLWGTRVGALAGGLGSALADLLTGYAHWAPFTLIIKGIEGFLVGAFASREQNLPK
                     RILFLSLAGLEMVGGYFLAGSLLYGPGVAIVEIPGNLVQAGSAVLLSSLVVYAIRRGE
                     KSIFHSI"
     gene            complement(6297..>6977)
                     /locus_tag="PKN31_04875"
     CDS             complement(6297..>6977)
                     /locus_tag="PKN31_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOA99291.1"
                     /translation="IAFDGDGDRVVFADEKGKILKGEEGIIFFLRNTLPHLPGGEKFV
                     YDLKCSQIVPQEVKRMGGIPIPERSGHAHIKRRLLRENAFMAGEISGHYFFRELKRDD
                     GLYAAALFLYYLSRTPYPLSQIRETFPPSHITPDIRIAAKGREDLLPLVENSLSGGEV
                     SYLDGIRVEWEEGWAMIRKSVTEPVYTLRFEGKTSQDIPRLVHRFLRSLPEVEEEVLR
                     HIKKQERN"
     assembly_gap    6978..7077
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7078..7406)
                     /locus_tag="PKN31_04880"
     CDS             complement(<7078..7406)
                     /locus_tag="PKN31_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOA99292.1"
                     /translation="MSIFKECDIRGIFPAEIDEEKTYLIGRAFASSLPAGSRIVVGGD
                     VRKSTPLLKEALIKGLLESGGEVVDLGVVPTPLLYFAVEKLQGEGGIMVTASHNPPEY
                     NGLKITWG"
     gene            complement(7407..8366)
                     /locus_tag="PKN31_04885"
     CDS             complement(7407..8366)
                     /locus_tag="PKN31_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOA99293.1"
                     /translation="MKRKKVLVIGGTGHIGSYLIPSLVAKDYEVVVFSRGKTAAYPEG
                     FWEKEIKMVTVDREKEERTGRWPELIEETGADIIVDIICFEPESAQILIDALTGKDVH
                     LLSCGTVWVYGKTRIVPTPESAPRFPENDYARKKAIIESKLLQATREGKVKATVIHPG
                     HITGPGKTFVTPFGDHNPENLQKIIDGEEIYLLDGGFSTLHHVHPQDVASLFLRAIEK
                     ETRAIGESFNCGASEAMTFWGLGEYFASLFGKEFYFQNISLEEYTEKFGYPEEAAQHV
                     RQGCCVSMLKAQEVLNFTPRYSAREAVALATDDLIKKGTLKVN"
     gene            8463..>9369
                     /locus_tag="PKN31_04890"
     CDS             8463..>9369
                     /locus_tag="PKN31_04890"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOA99294.1"
                     /translation="MEENWREKLEKAPLSRIMEVAYENQVLIPAFNVAYLPMVEAIME
                     ALRETETFALLEVSRPDITRFGAKSFQAVKEEYERFGDLAFSRLHQDHVPVIDEEGEK
                     VDWKSLIEEALFLGYHSVMIDGSRLPLNENIRVTRETVMLAHRRGVCVEGELGSVLGH
                     EKGPLPPYEELFQSGQGFTSPEDAQRFVQETGVDWLSVAIGNIHGAITGAAKDQKKVE
                     ARLNIQHLQQIARVTGIPLVLHGGSGIKREYVLEAIKNGITKINIGTEIRQAYERGLG
                     ENSNIEEAQKETKEKVKELITSYYEI"
BASE COUNT         2975 a         1548 c         2183 g         2563 t
ORIGIN      
        1 ttctctctac tgggttttgg ctgagtcaca acttagtata tacttaggat gtcagatgag
       61 gttattttga gaagaaaggg gggatgaaaa gatgaaagta cttcatacgg cagatatcca
      121 tttaagggaa tatgaagatg agaggtggaa ggcgcttcaa aagctgattg agattggaaa
      181 ggcagaaaag gttgagcttt ttgtaataag cggtgacctt tttgatagag ggattgacgc
      241 cgaaacttta agacctaaga taagggaaat tttctcgaat actggtttta aaatagtcct
      301 gattcctgga aaccacgatg atgaatcata taaaagcggt atgtactttg gcgaagacac
      361 aattatctta aacgacttaa atgagccttt tgaatataaa gatgtaagga tatgggggtt
      421 accttttgaa cccctggagg gggaaaaggt tgttgaaaag ctccgttcta tagctaataa
      481 tttaacttca gacaggaaga atattctact atatcacggt gaattgcttg atgctttctt
      541 ctccaggagg gattttggag aagaaggtga agagagatat atgccggtca aactatctta
      601 cttcaaagat ttgaatcttg attacatact tgccggacac tttcattccc gatttgatat
      661 gtggaagtta gaaaacggag gctactttgt ttaccctggc tcacccgtat caataactaa
      721 aagagagaca ggacggagaa aagtaaatat atttgaaatc gggcagccgc ccagggagca
      781 tcctcttgat acttttcatt ttgaggaagt ggtcatagaa ctggaccctt ttatggggga
      841 aaatccggta gagatagtaa aggaacgact taaggcatcc catcctgaag cgagtattat
      901 cttgacggtt aggggataca ttaacagtga agagattgaa atgagcgaat ctgagcttgt
      961 agagcaaata aaagaagtca ttaaggacaa accggtagaa gaatatcttg aattcaggga
     1021 catgtatacc atacttgaag atgacttgtt taaaagcttt atgagcaagc tcgcggagac
     1081 tccttttacc gaagagaaga aaaaagaatt gcttgacctc acgatacaag cgatgatgga
     1141 ggccaggtta tgagagtaaa ggaattctca ataatgcgat atggtccttt gtccaatacc
     1201 ggtagaatat tgctccatag cttcaatctg ttctggggta aaaatgagga tgggaaaacc
     1261 ctcaccatag atgccctggt aaagatgttg ttggggcgaa gtgtcaggga gtttgagcac
     1321 ataaatcggg tagaagaaaa tccggagggt tatgtaatcg tagaaagcga aaaaggtgag
     1381 gaaattaagc ttccagaaag aggagactta acaaagattg ctggtttgac ttcttcagaa
     1441 tgctgtaata ttttcatagt gaggaatagc aacctgtcta ttgcccgtga cgctgctcaa
     1501 gagagtaaat tttatatcga tgtgacggat cgtctcaccg gtcttagaac aggagaaatc
     1561 cgtagaataa aagaggctct caaagaaatg ggtaagataa cccctcaggg tgcatttaga
     1621 gatattagag atgaaaaatt aaaaacaaga atagaagatg cacaaaactt gatagagaaa
     1681 attaaaagcc tatgcaaaga aaccgaagaa aaaaggtttg acgaacttga aaaagaatct
     1741 gcagaattaa aggaagaaat agaaagaata gatcgggaaa tgaaagcatt tgaagatgcc
     1801 agaaagagag agaaatacga aaggggacga gaggcattag ataattttaa aagctctctg
     1861 gagcggctca aagacttgga agtttacaat gaagaagatg aaagattatg gagagattgc
     1921 catagagaca tcatgactct tgaagaagaa aggaaaaagg tagctgagga gcttgaggag
     1981 agtaaagaag agttcaagac aataaacgag aagttagacg aggcagagag agattttcgg
     2041 gttctttctg agagaaagaa aaatctggat gaggaaatta aaccggagtt aagggcttat
     2101 gaaaataaaa gtgtagagtt agtggagcaa caagaaaaag ataaggtttt cactttatta
     2161 gggataattt tcgcagtatt gctggggata tctttgcttg gagttatttt cagcccttcg
     2221 ccgttatttt atgctctggc aatattgtct tccgtcttgg ttttagtatc ggtagtgttt
     2281 caacttcggc tggtaaggga aaaagctcga tttgcgaaga tactggagga gaataaactg
     2341 tctctttcaa aattggggat agaagcagag agtgtggaaa agattctttt taatattcag
     2401 aggtttgatg agggttatca cagaaaattt gaagagctac aagacataag aaggaaaagg
     2461 gaaaacttag aagaaaaaat tgaaaatttg caaagtaaga aaattcctga gatagagaat
     2521 aaacttaaaa gccttgaaga aaaaatagaa gagataaaaa gaaaatcaaa agaagaatct
     2581 ttagaggaat acaccgagaa gctcaagtta aagcaaaaac tggaaaggtt attaggagag
     2641 caaagaagca tcttaaagag ccacctgggg gagaagggca aaagcttgga agaaaatatt
     2701 tcttactgga gcgaagaaat tgaaaaactt gagatatacg aggataaagc taaaaatgtg
     2761 aaatatagcg agactgctgt agtagatttg ggaaagaaaa aggcagaact ggaagagaag
     2821 ttaaaggaca taaatgagat tatgaaagcc attcgaaagg atttggatga catagagcga
     2881 aaggtgaacg aaattctgcg attagaagaa gaatatctgc actgtaaaac attagtggat
     2941 ttggaagcag ttaaagatga attgcaaaaa tttgttgatg aatgtgaaaa taacaggagc
     3001 aatgtattag aagttataag aatttttgaa gaaatagaag cagaagaaaa agaaaaggtt
     3061 tctgagcttt ttggcagggg gagttccacg tcaaaatatt ttggagagat aactgatggt
     3121 ctttatgaag aagttgtatt caatcgggaa gaagaaagaa ttaaagtcag gcgtaaggat
     3181 aatatgatgc tggaggcaga aaagttatcc ggtggcgcat atgaccagtt gtatttttct
     3241 atcagacttg ctctgggaga aaggcttctg gaaaataaaa aaggattttt tattatggat
     3301 gacccattca taaaagcaga cccggatagg ctacggagac agatcgaaac attaaagaga
     3361 attactgaat taggatggca ggttatgtac ttcagcgcca aaggggagat taaagaggct
     3421 ttgagagaag atatagactg tggcaacatt aactatgtag agcttccagg tatattagct
     3481 taaatatcag gtgatttaca tcggctttac tataccgggg gaagaaggtt aggcagttta
     3541 gaggatgtgc tttaattcct gaggtgtgtg tttgatgaga gccactccca tatttgagct
     3601 attttgctga gcttgtgttg atttctgctc cctttttccc attgctctgg ggaaagagaa
     3661 tagacactat actgcaagat ttattttgcc atcattctaa tcttttcagc tttaaacatg
     3721 ttcgttttgc agaagttaat ggtgctggga gatggctggg aaataaaagc gcgagaaaac
     3781 ttaagaacgg gttctttatt actcaaaaag atgggcatca gcatctcagg caaactttta
     3841 ctgctgatga agttcaataa tgcaattgga aaattgtata attatatcag caatatagca
     3901 tgcatccccg atatagagga atgggagcag aaaaggtagg cgccagaaga gtggtgctcg
     3961 atgttgaaag cgataatatc agcactattg ctttccctgc attttaacag ggtgataaaa
     4021 gaaatctgag atggcaaaaa agataattgc cagcagaaaa tagagctatg ttggattaac
     4081 cactcatttc ccggggtgtc ataatgatcc tcgtttgctc atctgcccga agtagtcatt
     4141 acgagttcgc attttcgctt acgcggcaat cccgagtttt taacccgatg tagcttactt
     4201 ttagcgtttg ttgaggctca aaaaggaaag atcaccacgg gagacgaata agcgaccctc
     4261 gcgatgacag gaaaagaggt tcctcgcaaa agagaagggg ggtgataagt ggatttaacc
     4321 tttatagttc tggattcctg atagagatat tcagaaatga caacaaaaaa aagacaccct
     4381 caccctcacc ttaaccctct cccgaggagg gtaaggggaa aatagttcag gggtgatggt
     4441 aaaagcaagg ggttgataaa gggaaattct tttccccttt atagtctact gtattattct
     4501 gtttacgatt gcattgagaa aatttataat attgctcaat taatggatgg ctcggactcg
     4561 ttatgacctg taaataatga gggggcaggt ggctcgtttt attttgttct aattctgtaa
     4621 agctagactt cacaaatcta ggaaacgtca gtaaaatatg taacaattta aaaataggga
     4681 gaaccttcag tgaaaaagaa atttgataga gtgtatcagt tcaagattac tctcaagggt
     4741 atcagaccac caatctggcg gagaatacac gttccggaaa cttatacctt ttgggactta
     4801 catgtggcta ttcaagatgc aatgggctgg gaagatgccc atcttcatga atttgaaatg
     4861 gtctctcctt ctacaggaga aaaggtgagg ataggaactc caactgaaga agccgaggtt
     4921 tttgggcaac aaattatccc ggaagaaaag cagaagattg ctaactggtt ttctatggaa
     4981 aacagggtgg caagttatgt ttatgatttt ggagatgatt gggaacataa gatagaatta
     5041 gaaaaaatac ttcctcggga gaaaaatatt aagtatcctg tatgcatagc cggcaaaaga
     5101 gcctgtcctc ctgaagattg tggaggcgta cggggatatg aagaattttt agaggctata
     5161 agaaatcctg aacatgagga acacagtata atgttagaat ggattggtgg agaatttgac
     5221 cctgaatatt ttaatccgga agaagttata tttgaagacc ctgatgaacg tcgaaaatat
     5281 ttatgggaat aataggaaca ataacccaca taacttaaca aaagggcaaa gagagggtat
     5341 aaaggtatga atagcgggga aaagagatga gagatttcat aaaagaggca gagaaattat
     5401 taattgctgc cagacatgac ctgaatatgg gagattacta ttcctgtttt tcaaaaagtt
     5461 atcatgccat ggtcctttca ataaaggcgt cactgagctt attcgagggg cgttttagca
     5521 aaacggaaat agaaatgctt aaccgagatg tagaaaaaaa gtttaaggat attcatgatc
     5581 agatattact tatggataat ggggcaggct ttatcttaaa tgagaaagaa gtaaaagagt
     5641 ttttagaaga agcaggtgat tttgtccaaa agtcaaagaa tgatcttgaa gagtggttac
     5701 agacaaaggg agagaaataa tctgtcattt ccctgttaat ggtagctgtg ataaaatagc
     5761 caaatagatg aaccgcgaaa aggagattga aatacgatga gagagtcaac tcgtactttg
     5821 acttattcag ctatagctat cgcgttagtg gctatagcca ctatgagttt gcagattcct
     5881 attcctcaga ctcgaggata tataaatctg ggagatacct taatatttgt ttttgccctg
     5941 ctctggggaa ctcgagtggg ggctttggct ggaggattag gctctgcttt agctgacctt
     6001 ctgaccgggt atgctcactg ggctccgttt actctgataa ttaaagggat agaaggtttc
     6061 ttggtgggag cttttgccag ccgggagcaa aatctcccta aacggattct ctttttatct
     6121 ctggctggtt tagagatggt gggaggatat tttttggctg gctctcttct ttatggacct
     6181 ggtgtggcaa tagtggaaat cccgggcaat ttagtgcaag ctggaagcgc ggttcttcta
     6241 tcctctcttg tagtatatgc catccgcaga ggggagaaat ctattttcca tagcatttag
     6301 tttctttcct gcttttttat atggcggaga acttcttctt ctacctcagg tagagatcgc
     6361 agaaaacgat gaactaagcg gggaatatct tgagaggttt ttccctcaaa gcgaagggtg
     6421 tatactggtt cggtaactga tttgcgaatc attgcccatc cttcttccca ctctactctt
     6481 attccatcga gataggacac ttctcctcct gaaagtgaat tttccacgag agggagcagg
     6541 tcttctctcc ctttagcagc aatccgaatg tcgggagtga tatgagaggg aggaaaagtt
     6601 tcccttattt gagaaagagg gtatggtgtt cgggaaagat aataaaggaa tagagctgca
     6661 gcatatagcc catcatctct ttttagttcc cggaaaaagt aatgaccgct gatttcccct
     6721 gccataaagg cattttcccg gaggaggcgc cttttaatat gagcatgccc agaacgctcc
     6781 ggaataggta tacctcccat tctttttacc tcttgaggga caatctgaga acattttaag
     6841 tcatagacaa atttttctcc cccgggaagg tgaggcaagg tgtttcgaag gaaaaaaata
     6901 attccttctt ctccttttaa aatttttccc ttctcatctg cgaacactac tcgatcccca
     6961 tccccatcaa atgctatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     7081 caggtgattt taagcccgtt atattctgga ggattgtggc ttgcggtaac cataatccct
     7141 ccctctcctt gtagcttttc tacagcaaag tataaaagag gggtagggac aacccccaga
     7201 tcaactactt ctcctccact ttctaaaagt cccttgataa gggcttcttt aaggagagga
     7261 gtgcttttcc gcacatctcc tcccactact attctggaac ctgcaggaag cgatgaggca
     7321 aaagctctcc cgatgagata agttttttct tcgtcgattt ctgcagggaa aattcctctg
     7381 atatcacact ctttaaaaat actcattcaa ttcaccttta gtgttccttt ttttattaaa
     7441 tcatcagtag ccagagctac tgcttctctt gccgaataac ggggagtgaa atttaaaact
     7501 tcttgagctt ttaacatgga tacacagcaa ccctgtcgga cgtgttgagc agcttcttcg
     7561 ggataaccaa atttctcagt gtattcttct aaagaaatat tttgaaaata aaattctttt
     7621 ccaaaaagag aggcgaagta ttctcccaat ccccaaaagg tcatagcttc agaagctccg
     7681 cagttgaagc tttctccaat agctctggtt tctttttcta tagctcttag aaaaagagag
     7741 gccacatctt gagggtgaac atggtgaagg gtggagaacc ctccatccag cagataaatt
     7801 tcttctccat cgattatttt ttgtagattt tctggattat ggtctccaaa aggagtaaca
     7861 aaagtttttc ctggaccggt aatatgtccg gggtggatga cggtagcctt aaccttgccc
     7921 tctcgtgtag cctgcaaaag cttgctctct attatggcct ttttacgagc ataatcgttt
     7981 tcgggaaacc gaggagcgct ttcaggagtg ggaactattc tggttttgcc atatacccac
     8041 actgtaccgc aggaaagaag gtgaacatct tttccagtta gagcgtctat aagaatttga
     8101 gcgctctccg gctcaaaaca tataatatct actataatgt ctgctcctgt ttcttctatc
     8161 aactctggcc agcggccagt tctctcctct ttttctctat ccactgttac catctttatc
     8221 tctttttccc agaaaccttc cgggtaggca gcggtttttc ctctggaaaa taccaccacc
     8281 tcatagtctt tagcgaccag agaggggatt agataactac caatatgccc cgtgcctcct
     8341 ataaccagaa cttttttcct tttcatattt tcctccttta ctttatcatt ttatcctttt
     8401 atagttatat ttaaaaggta gttaattaaa aatagttaat aaaagaaagg agagcgataa
     8461 acttggagga aaactggaga gaaaagttag aaaaagcacc tctttcccga ataatggagg
     8521 tggcttatga aaaccaggtg ttaatccctg cttttaatgt tgcttatctt cccatggtgg
     8581 aggctattat ggaggcatta cgagagaccg agacctttgc tcttttagag gtttctcgtc
     8641 ctgatatcac tcgttttgga gcaaagagtt ttcaagcggt aaaagaggaa tatgagcgct
     8701 ttggagacct tgcttttagc cgactccatc aggaccatgt cccggtgata gatgaggaag
     8761 gagaaaaggt agactggaag tcgctcattg aagaagctct ttttctggga tatcactcag
     8821 tgatgattga tggctctcgt ttacctttga atgaaaacat ccgggttact cgagaaacag
     8881 taatgcttgc tcatcgccgt ggagtttgtg tagagggaga attgggctcg gttctgggac
     8941 acgaaaaagg ccctcttcct ccttatgagg agctttttca gagcgggcaa ggatttactt
     9001 ctcctgaaga tgctcaacgt tttgttcaag aaactggcgt agactggctt tcggtggcta
     9061 ttggtaatat tcatggagca atcaccggcg cggcaaaaga ccagaagaaa gtggaagctc
     9121 gattgaatat ccaacatcta caacaaattg cacgagtgac cgggattccc ctggttcttc
     9181 atggaggttc gggaattaaa agagaatacg ttttagaagc gattaaaaac ggtattacca
     9241 agattaatat aggaactgaa atccgccagg cttatgagag aggtttaggg gaaaattcta
     9301 atatagaaga agctcagaaa gagacgaagg aaaaagttaa agagctgata acttcctact
     9361 atgaaatag
//
LOCUS       DAOPSS010000060         9355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6167_length_9355_cov_9.205591, whole genome shotgun sequence.
ACCESSION   DAOPSS010000060 DAOPSS010000000
VERSION     DAOPSS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6167_length_9355_cov_9.205591"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1627
                     /locus_tag="PKN31_04895"
     CDS             116..1627
                     /locus_tag="PKN31_04895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOA99295.1"
                     /translation="MSYLGIDVGTTGCKVVAFDEEGKIVALSYREYPLYYPQTGWIEL
                     DAEEVYQAVEDSLLEISQKLRHDPPCSLAISSQGEAVVPVGEKGECLARSIVTFDQRG
                     DEFVPFWEEKIGRKRFFELTGTSLSGIGTVNKILWWKKYKQEVFEKARYFLCFEDFIV
                     HRLGVEPAISFSLAGRTMMFEVKKEKWSEEILSLAGIDPARLARPLPSGEVVGEVASS
                     FAQKMGWKEKVIVATGGHDQPCGALGSGVIKPALAMDATGTVECIAPALGELVLSPVM
                     EENNLCCYHHAFPSLYLTMVYNFTGGALLRWFRDNFAQKEKEIAERENCDVYDLILAR
                     IPSQPTNIWVLPHFTTTGTPYFDTQSAGVIVGLKLETSREEFIKALLEGVSYEMKFNL
                     ELLSQAGVKVERLRAVGGGAKSPAWLQIKADVYGLPVEALSVTEAASLGAALLGRRAK
                     EGIEDFAGLINKLVKVREVYEPDKERNALYEERFEVYKRIYPALKNHIWKGEK"
     gene            1624..2676
                     /locus_tag="PKN31_04900"
     CDS             1624..2676
                     /locus_tag="PKN31_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HOA99296.1"
                     /translation="MTLKAAFFLGPEKMEIREVEVPQVEEGEVLLRVAACAICGTDVR
                     IFHHGHSHVQPPQTTGHEIAGEIVEVGKGVEGYKVGDKVVVITVISCGRCRYCRRGLQ
                     NLCPEQKYIGYDYPGGFAEFLKIPREGVERGNLLVLPPDADLLEASLVEPLSCVVNGQ
                     SYLNIGFGENVLVIGAGPIGCMHVELARNRGAGKIFLADISQERLKLAQVVEADEYIN
                     SQEENLTQRILELTDNLGADVIIVAASSGSAQEEALEMVAPQGRISFFGGLPRENPTI
                     KFDSNVVHYKEVGVFGVFASHASQYEEAAKLIYSGRVRAKRLITHVLSLEEVVKGIDL
                     VRQGIALKAVVSMENN"
     gene            2692..3480
                     /locus_tag="PKN31_04905"
     CDS             2692..3480
                     /locus_tag="PKN31_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOA99297.1"
                     /translation="MDPQPLARKVAIVTGSASGIGKGIALRFAEEGADVSCWDLNLEG
                     AKETAREVEKAGRKSLALQVDVTKSGEVEDAAKAVFDQFGKIDILVNSAGIIRAHFIT
                     EVPEEIWDSIITINLKGTFLCIREIAKYMIPQNSGKIISISSKSGKKGGLWLGAYSAS
                     KFGIIGLTQSVAMDLAPYKINVNAICPGNVFSTPMWDLLDKEYSKKLGVPPQEVRKIY
                     VEKVPLGRECTVEDVANVAVFLASSYSDYMTGQAINVTGGQEVH"
     gene            3461..4477
                     /locus_tag="PKN31_04910"
     CDS             3461..4477
                     /locus_tag="PKN31_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99298.1"
                     /translation="MDRRFTELNKIGLVVNPAAGKDIRRLIAYGSVFGNQEKVNYTLR
                     LLRGIESVVSEPVEVMYMPDPYDLVGSVEDDFPSGKIIFHRAPIPVFGDEVDTLNFTD
                     WAVNEEKVDALVVVGGDGTNRIVAKKSALTPLFSLFGGTNNVFAENIEPTVMGLAVGF
                     FLENYSLREKVVKKSKILRARSKEAGKEELALVDVVVVEKASVGARAVWEPELVRLIV
                     VTQSSPLKIGLSSVVGRLISISPEEERGAVVELGEGGEMVKAPIAPGLVEEIKIRRWE
                     FLPLGASISLPCQRGVLALDGEREIILREGENWEIRLEDTGPRRVDVEKLMSLVQERS
                     VING"
     gene            4470..5687
                     /locus_tag="PKN31_04915"
     CDS             4470..5687
                     /locus_tag="PKN31_04915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOA99299.1"
                     /translation="MAKKVIMPKLGLTMEEGIINKWLVKEGDRVNKGDVLFEVATDKV
                     NMEVESPTEGVVLKILYPEGATVPITEAVAYIGKEGEEIPEEEEKPEEKIEAVKMEEE
                     KVEEKRGEAPLPSETERIRVSPVARRLAEEHGIDLSQIQGSGPGGRIVREDVEKAIEE
                     KEKERKLIVEEVKVPREKERIPLSRMRKIIAQRLTESYQSTPHFFLHQEICADELVKM
                     REKILPLVEKETGLRVSYTDILVKMVAKAVEKFPLFRAHLAGEEIEISPEVNIGVAVA
                     LEEGLIVPVVKNANEKGISRITQEINALAQKARSNQLTPEEVSGGVFTVSNLGMWGVD
                     SFTAIINPPEAAILACGAIRKKPFFNGKEIVPAYLWELSLSCDHRLIDGALAAQFLQF
                     LKNLLEEPFTLII"
     gene            5700..7223
                     /gene="xylB"
                     /locus_tag="PKN31_04920"
     CDS             5700..7223
                     /gene="xylB"
                     /locus_tag="PKN31_04920"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOA99300.1"
                     /translation="MEYFLGIDVGTTGCKVVLVNEKGELISKAVAEYPLYTPYPNWAE
                     QDPADWWQGTVQAVKEALQMAEIDPLLVQGVGLTGQMHGSVFLDEKGEVLRPAILWCD
                     QRTARECEEITQIVGEEKLMSINCNPVLPGFTAPKILWLKNNEPHLYEKVAKVLLPKD
                     YIRFLLTGDFATDVSDASGTSLFDVPRRKWSEELMSLLEFPPSWFPPSFESPEVTGYL
                     KKEVAEMWGMGDKVLVVAGGGDNAAGAIGTGIVKPGLVSASLGTSGVVFAFSDEVKVD
                     KKGRVHTFCHAVPGKWHVMGVMLSAGGSLRWFRDRLGMEEKNLASLLEKDSYDILTAE
                     AEKALPGAEGLIFLPYLTGERTPHADPYARGVFFGLTLKHRKNEMVRAVMEGVTFGMR
                     DSFEIMREMNIPIQEVRAIGGGARSALWRKIQASIYEVPLWGVKIDEGPAFGAALLGA
                     VGRGVFSSVEEASEKAVKVTEQVLPSPELVDYYNKAYRLYRDLYPALRDFYPRLQEL"
     gene            7239..8222
                     /locus_tag="PKN31_04925"
     CDS             7239..8222
                     /locus_tag="PKN31_04925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOA99301.1"
                     /translation="MDKKAVCLGELLIDFVSTVSGVTLKDAPGFEKAPGGAPANVAVG
                     LAKLGIDTHFIGKVGRDAFGEFLREVLEKNGVNTRFLFSTEKAKTTLAFVSLTQEGER
                     DFVFYRDPGADMLLDQREIGEECFQGKGVFHFGSITMTHEPAYSATMRAISLAKKYGY
                     LLSFDPNLRPALWRDLEEARFRMRQALKSVDILKVNEEEARFLAGTSDLEQALKFLSQ
                     NFSLALIALTLGKRGCLIVHGDNRLEMEGFSVATVDTTGAGDGFVAGLLSSLYMFWED
                     LRERKEIPESAIAHAARRANAVGALTTTKKGAIPALPTSEEVEIFLKEHPL"
     gene            8219..9157
                     /locus_tag="PKN31_04930"
     CDS             8219..9157
                     /locus_tag="PKN31_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOA99302.1"
                     /translation="MIFVLCANPALDRLLIMEELSLNEVNRASQVTETVGGKGINVAR
                     AIKQKGGNPRLLIFEGGSTGEKIKKGLEKENFLFTSWPSDETRITTIIHEEKTKRHTV
                     INEPGPLVSISTTLACLHFLENNLRKGDYLVLSGSLPRGVRLDFYALLIQISKRKGAF
                     SVLDTSGEPLRRALPAAPFMIKPNVREAQEALGFSISSWEDKIKAVKIFENQGVELVI
                     LSDGKRGVVATYRGEIWKVGMKKEVEGGGYSIGSGDTLVGVVVEYLEKGKSIEESLAA
                     GVSCGLANTFYPGGGIFDEEKAEELEKDILIERIKD"
     gene            9171..>9355
                     /gene="raiA"
                     /locus_tag="PKN31_04935"
     CDS             9171..>9355
                     /gene="raiA"
                     /locus_tag="PKN31_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOA99303.1"
                     /translation="MNIVVQGRNMEISEVMRNYVTKKLSKLERFFDRLMDATVNFTLE
                     RGRVKVESTLTASGVVIR"
BASE COUNT         2652 a         1624 c         2585 g         2494 t
ORIGIN      
        1 tttagaaata gtccagaaaa tagataagca gaaactggag gagattcaaa aagaagccga
       61 tgctctatca gctcttcggt atataaagag tttgctttag agaggaggag agaagttgag
      121 ctatctgggg atagatgtgg gaaccacggg ctgtaaagtg gtggcctttg acgaagaagg
      181 aaagattgtg gccctctcct atcgagagta tcctctttat tacccgcaga cgggctggat
      241 agagttagat gctgaggagg tttaccaggc ggtagaggat agcttgctgg agatatcaca
      301 aaagctgcgt catgaccctc cttgcagtct ggccatttcc tctcagggag aagcggtggt
      361 tccagtagga gagaaggggg agtgtttggc gcgcagcatt gtcacttttg accagcgagg
      421 ggatgaattt gttcccttct gggaggagaa aattggcaga aagcgctttt ttgagctcac
      481 cgggacttct ctttccggga ttggaacggt gaataaaata ctatggtgga aaaagtataa
      541 acaggaagta tttgaaaaag cccgctattt tttatgcttt gaagatttca tagtgcaccg
      601 cttaggagtg gaaccggcta ttagtttttc cttggcgggg agaactatga tgtttgaggt
      661 taaaaaagaa aagtggtcag aagaaattct ttctttggcg ggaattgacc cggcgcgcct
      721 ggctcgccct cttccttctg gggaggtggt gggagaggta gcttcttctt ttgctcaaaa
      781 aatgggatgg aaggaaaaag tgatagtagc caccggaggt catgaccagc cttgtggagc
      841 tttgggttca ggggtgatta aaccggctct ggctatggat gcgacaggaa ctgtggaatg
      901 catagctcct gcgttggggg aactggttct cagcccggtt atggaggaaa acaacctgtg
      961 ttgttatcac cacgcttttc cttctcttta tcttaccatg gtttataatt ttaccggagg
     1021 agcactcttg cgctggttcc gggataattt tgctcaaaaa gaaaaggaaa tagcagagag
     1081 agaaaattgt gatgtatacg acctcattct cgctcgtatt ccatctcagc ccaccaatat
     1141 ctgggtttta cctcatttca ccactactgg cactccctac tttgatactc aatctgctgg
     1201 ggtaattgtg ggactcaagt tagaaacctc tcgagaagaa ttcataaagg cgcttcttga
     1261 aggggtgagt tatgagatga aatttaactt agaacttctc tctcaggcgg gagtaaaggt
     1321 agagaggtta agagcggtgg gaggaggagc caaaagccct gcctggttac agataaaagc
     1381 tgatgtttat gggttaccgg tagaagctct gtctgttacg gaagccgctt ctctaggagc
     1441 ggccctttta gggagaagag caaaagaggg aatagaagat tttgccggtt tgattaataa
     1501 attggtgaag gttagagaag tttatgaacc agataaagaa agaaatgcac tatatgaaga
     1561 gaggtttgaa gtgtataaga gaatttatcc ggctttaaaa aaccatatct ggaaaggaga
     1621 gaaatgactt tgaaagctgc tttttttctg ggtccggaaa aaatggaaat tagggaagtg
     1681 gaagtccccc aggtggaaga gggagaagtg ctccttcggg tggcggcctg tgccatctgc
     1741 ggaactgatg tgcgaatttt ccatcatggt cattcccacg ttcagccccc tcagaccacc
     1801 ggtcatgaaa tagctgggga gattgtggag gtgggaaagg gagtagaagg atataaggtg
     1861 ggagataaag tagtggtgat taccgttatt tcctgtggcc gctgccgtta ttgccggcgg
     1921 ggtttacaga acttatgtcc ggagcaaaag tatattggtt acgattaccc gggtggcttt
     1981 gccgaatttc tgaaaattcc ccgggaaggg gtagagagag gaaatctctt agttcttccc
     2041 cccgatgccg atcttttgga agccagttta gtggagcctt tgtcttgcgt ggtcaatggt
     2101 caatcctact tgaatatcgg tttcggagaa aatgttttag tcattggagc aggacccatt
     2161 ggctgcatgc atgtggagct ggctcgaaac cgaggagcag ggaagatatt tttagctgat
     2221 atttcccagg aacggttaaa gttggctcaa gtagtagaag ccgatgagta cattaactct
     2281 caggaggaaa atctcaccca gagaatactt gaacttaccg ataacttagg agcagatgtg
     2341 ataatagtag cggcctcatc tgggagtgct caagaggagg ccctggagat ggtagcccct
     2401 cagggaagaa tcagcttctt tggaggatta cctcgagaaa atcccaccat taaatttgat
     2461 agtaatgtcg ttcactacaa agaagtggga gtttttggag tttttgcttc tcatgcttct
     2521 caatacgaag aggcagctaa actgatttat agcgggcgag tgagagctaa acgtctcatc
     2581 actcatgttt taagcttaga agaagtggtc aaaggaattg atttagtgcg ccaaggtatc
     2641 gctcttaagg cggtagtatc tatggaaaac aattaaaaag aggtgaagaa aatggatccc
     2701 cagcctctgg ctcgtaaagt agccatagtg actggttcag cttcaggtat tggaaaaggc
     2761 attgcccttc gttttgccga agaaggggca gatgtatctt gctgggattt aaacttagaa
     2821 ggagcaaaag aaactgcccg ggaagtggaa aaagcgggga ggaaatctct ggctctccag
     2881 gtggatgtta ctaaaagtgg agaagtagaa gatgccgcaa aagcagtatt tgaccagttt
     2941 ggtaagattg atattctggt aaactcagca ggaattattc gggctcattt tataaccgaa
     3001 gtcccggaag aaatctggga tagtataatt acgataaact taaaaggaac ttttctctgt
     3061 ataagagaga tagccaaata tatgattcct cagaatagtg ggaaaataat cagcatcagc
     3121 tctaaatcgg ggaaaaaagg agggctctgg ttaggtgctt attcggcttc taaatttggt
     3181 ataattggct taactcaaag tgtggccatg gatttagcac cttataaaat taatgttaat
     3241 gccatttgcc cgggtaatgt tttctccact cctatgtggg accttctgga taaagaatac
     3301 tccaaaaaat taggagttcc tcctcaggag gtgaggaaaa tatatgtgga aaaagttcct
     3361 ctgggtagag aatgcacggt agaagatgta gctaatgtgg cagtattttt agcctcttct
     3421 tattctgatt atatgaccgg tcaggctatt aacgtcaccg gtggacagga ggttcactga
     3481 gctgaataaa attggattgg tggttaaccc cgcagcagga aaagatatcc ggcgattgat
     3541 agcctatggt tcggtttttg gcaaccagga aaaggttaat tatactcttc gcttgcttcg
     3601 gggcatagag agtgtagtga gcgagccggt tgaggtaatg tatatgcctg atccttacga
     3661 tttggtagga tcggtggaag atgattttcc ttccgggaaa attatcttcc accgtgctcc
     3721 tattccagtt tttggtgatg aggtggatac tttaaatttc accgactggg cagtaaatga
     3781 ggaaaaagta gatgctttag tggtagtagg aggagatgga actaaccgga tagtagccaa
     3841 aaagagtgcc ctcactcccc ttttctctct ttttgggggt actaataacg tatttgctga
     3901 aaatattgag cctacagtta tgggactggc ggtgggtttt tttctggaaa attattcttt
     3961 gcgggaaaaa gtggtgaaaa aaagcaaaat actgagggcc agatccaagg aagcggggaa
     4021 agaggagtta gctcttgttg atgtggtagt ggtagaaaaa gcttcagtag gagcaagagc
     4081 ggtgtgggag ccagagttag ttcgtttgat agtggtaacc cagagcagcc ctttaaaaat
     4141 tggcttgagt tcggtagtgg gaagattgat tagcattagc ccggaagaag aaagaggagc
     4201 agtggtggag ttaggagagg gaggggaaat ggttaaggct ccgatagctc cgggtttagt
     4261 ggaagagata aaaatccgga gatgggaatt tttacctctg ggagcttcta tttctctccc
     4321 gtgccagaga ggagttctag ctttagacgg ggaacgggag ataattttgc gagaagggga
     4381 gaactgggag attcggctgg aagataccgg gccccggagg gtggacgtgg agaaattaat
     4441 gagcctggtg caagaaagga gcgtcataaa tggctaaaaa ggtaatcatg cccaaattag
     4501 gtttaaccat ggaagaagga attatcaata aatggttagt aaaagaagga gatcgggtaa
     4561 ataagggaga tgtcctcttt gaagtggcta ctgacaaagt taatatggaa gtagaatctc
     4621 ccactgaagg ggtggttcta aaaattcttt atcctgaagg ggctactgtt cctatcaccg
     4681 aagcggtagc ttatataggg aaagaagggg aggagattcc cgaagaagag gaaaaaccgg
     4741 aagagaaaat agaagcggtg aagatggaag aagaaaaagt agaagaaaaa cgaggagaag
     4801 ctcctcttcc ttcggagact gaaagaatta gagtctctcc tgtggctcgt cgcttagccg
     4861 aggagcacgg aatcgacctt tctcaaatac agggtagcgg cccgggagga agaattgtaa
     4921 gagaagatgt ggagaaagct atagaggaaa aagaaaaaga gagaaaattg atagtagaag
     4981 aagtaaaagt cccgagagaa aaagagcgta tacctctttc tcgaatgaga aaaatcatag
     5041 cccagcgcct gaccgagagt tatcaaagca ctcctcactt tttccttcat caggaaatat
     5101 gtgctgacga gctggtaaag atgagagaaa aaattcttcc tctggtggaa aaagagactg
     5161 gtttgcgagt ttcttatacc gatattctgg tgaaaatggt ggctaaagcg gtggagaaat
     5221 ttcctctctt ccgtgctcat cttgcaggag aagaaataga gataagccca gaagtgaata
     5281 tcggagtggc agtagcactg gaagaagggt tgatagttcc ggtggtgaag aatgctaacg
     5341 agaaggggat atcccggatt actcaagaaa ttaatgctct ggcgcaaaag gcgagatcta
     5401 accagttaac tccggaggaa gtttccggag gagtgttcac tgtttctaac ttgggaatgt
     5461 ggggggtgga ttctttcacc gccatcatca atccccccga agcagctatt ttagcctgtg
     5521 gggcaatccg taaaaaacct tttttcaacg gaaaagagat tgttcctgct tacttgtggg
     5581 agttgagcct ctcttgtgac catcggttga ttgatggagc gctggctgct cagtttctgc
     5641 aatttttaaa gaatcttctt gaggaacctt ttactcttat tatctgagga gggatgaaga
     5701 tggaatattt tttgggaata gatgtgggaa ctacaggatg taaagtggtt ttggtgaacg
     5761 aaaaagggga attgatatct aaagcggtgg cagaatatcc cctctatact ccttacccca
     5821 actgggcaga gcaagaccct gctgactggt ggcagggaac tgtacaggcg gtaaaggaag
     5881 ctttacagat ggcagaaatt gaccctcttc tggtacaagg agtgggactt accggacaga
     5941 tgcacggttc agtatttcta gatgaaaaag gagaagtttt acgcccggca attctgtggt
     6001 gtgatcagcg cactgcccgg gaatgtgaag aaattactca gatagtgggg gaagagaagt
     6061 taatgagtat caattgcaac cctgttcttc ctggctttac tgctcccaag attttgtggt
     6121 taaaaaacaa tgaacctcat ctatatgaaa aagtagctaa agttcttctc cccaaagact
     6181 atatccgctt ccttttaact ggagattttg ctactgatgt ttctgatgct tcgggaactt
     6241 ctcttttcga tgtgcctcgt cgtaagtggt cagaagaatt aatgtccctt ctggaatttc
     6301 ctccttcctg gtttcctcct tcttttgaat ctccagaagt taccggatac ctgaaaaaag
     6361 aagtagcgga gatgtggggg atgggagaca aggttttagt ggtggcaggg ggaggggata
     6421 acgctgccgg ggcaataggc acgggtattg tgaagccggg actggtttct gcgagcctgg
     6481 gaacttcggg ggtggttttt gccttcagcg atgaggtgaa agtggataaa aaaggaagag
     6541 tgcacacttt ctgccacgcg gttcccggta aatggcatgt tatgggagtg atgctttctg
     6601 caggcgggtc gctgcgctgg tttagggata gactgggtat ggaagaaaaa aatctggcat
     6661 ctcttttaga aaaagatagc tacgatattt taactgctga ggcagaaaaa gctcttcctg
     6721 gggcggaagg cttgattttt ctcccctatc tcaccggaga gcgcactcct catgctgacc
     6781 cttatgctcg aggggtgttc tttggactta ctctgaaaca ccgcaaaaat gagatggtaa
     6841 gagcggtgat ggaaggagtt acttttggta tgcgtgactc ttttgaaatc atgagggaga
     6901 tgaatattcc cattcaggaa gtcagagcta ttgggggagg agcacgcagt gctctgtgga
     6961 gaaaaatcca ggcttctatt tatgaagttc ctctctgggg ggtaaaaatt gatgaaggac
     7021 cagcttttgg cgctgctctt ctgggggcgg tgggaagagg agtattttct tcggtggagg
     7081 aggcttcaga gaaagcggta aaagtgaccg agcaggttct tccttctccg gaattggtgg
     7141 actattacaa caaggcttat cgtttatatc gcgaccttta tcctgctttg cgagattttt
     7201 atccccggtt gcaagagcta tagagggagg gatgatgagt ggataaaaaa gcggtttgcc
     7261 tgggagagct cttaatcgac tttgtttcta cagtgagcgg ggttacctta aaagatgctc
     7321 ctggttttga gaaagctccc ggaggtgcac cggccaacgt agcggtgggc ttggctaaac
     7381 tgggaatcga tactcatttt atcggaaaag tggggaggga cgccttcggg gaatttttga
     7441 gggaagtgct ggagaaaaat ggagtgaaca cccgttttct cttttctacc gagaaagcta
     7501 aaactactct ggcatttgtt tctcttaccc aggaaggaga aagggacttc gttttttacc
     7561 gggacccggg ggcagatatg ctattggacc agcgagaaat aggagaggag tgctttcaag
     7621 gtaagggagt ttttcatttt ggctctatta ctatgactca tgaaccggct tacagtgcga
     7681 ctatgcgagc tatttccttg gctaaaaaat atggttatct tttatctttt gatcctaatc
     7741 ttcgtcccgc tctgtggaga gatttagaag aagctcgttt ccggatgcgc caggctttaa
     7801 aatcggtaga tattttgaaa gtcaacgaag aagaagcgcg atttttagcc ggaacttcag
     7861 atttggagca agcgctaaaa tttctttccc agaacttctc cttagctctc attgctctta
     7921 ctctggggaa aagaggctgc ttaattgtgc acggggataa taggttagaa atggaaggat
     7981 tttctgttgc tactgtggac accaccggcg ccggtgatgg ttttgtggct ggccttttga
     8041 gttcccttta tatgttctgg gaagatttga gggagagaaa agagattccc gaaagcgcta
     8101 tcgctcatgc tgctcgtcga gctaatgctg tgggagcgct gaccactacc aaaaagggag
     8161 caattcccgc tctacctacc agtgaggagg tggaaatctt cttaaaggag catcctctat
     8221 gatttttgtt ctttgtgcca atcccgcttt agaccgctta cttattatgg aagagctgag
     8281 cttgaacgag gtaaatcgag cttctcaagt aacagagact gtaggaggaa aaggaattaa
     8341 tgtagcccga gcgataaagc aaaaaggagg gaatcctcgt cttctcattt ttgaaggggg
     8401 aagtaccggg gaaaagatta aaaaaggttt agaaaaagaa aattttttat ttaccagctg
     8461 gccttcagat gaaactagaa tcaccactat tattcacgaa gaaaagacca aaagacacac
     8521 agtaattaat gaacccggac ctctggtttc tatttctacc actttagcct gtcttcactt
     8581 tttagaaaat aatctccgaa agggagatta tctggtactg tctgggtctc taccccgggg
     8641 agtaagatta gatttttatg cccttttgat tcaaatttct aaaagaaaag gagccttttc
     8701 ggttctggat acctcaggag agcctttgcg tcgagcgcta cctgctgctc cttttatgat
     8761 aaagcccaat gtgagagaag cacaagaagc tctgggtttt tctatttctt cctgggaaga
     8821 taaaataaag gcagtgaaaa ttttcgagaa tcaaggggta gaattggtga ttctctcaga
     8881 cgggaaaagg ggggtagtag ctacctaccg gggagaaata tggaaagtag ggatgaagaa
     8941 ggaagtagaa ggaggaggtt attctattgg ttcaggtgat actttagtag gagttgtggt
     9001 agaatatttg gagaaaggaa aaagtataga agaatctctt gctgcggggg tgagttgtgg
     9061 tttagctaat actttttatc ctggaggggg gattttcgat gaggaaaaag ctgaagagtt
     9121 ggaaaaagat attttgatag aacgaataaa ggattaggag gcggcaggcg atgaatatag
     9181 tggtgcaagg aaggaacatg gaaattagtg aagtaatgag aaattatgtt actaaaaaac
     9241 tctctaagtt agaacgcttc tttgaccgcc ttatggatgc tacggtcaat tttaccctgg
     9301 agcgaggaag ggttaaggta gaatctactc tcaccgcaag cggtgtagta attcg
//
LOCUS       DAOPSS010000061         9304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6214_length_9304_cov_8.992756, whole genome shotgun sequence.
ACCESSION   DAOPSS010000061 DAOPSS010000000
VERSION     DAOPSS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6214_length_9304_cov_8.992756"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2444)
                     /locus_tag="PKN31_04940"
     CDS             complement(<1..2444)
                     /locus_tag="PKN31_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99304.1"
                     /translation="MSFCFIWFYGQKRVIDYFTIRFPSELEKKVKSEINIEKFSFRFP
                     NQLRLQGVSGRGEDFSFFVSRAILQWDWRYFFGASPSEKAFYLFLEEVELSSSQKFLQ
                     KLPSFSLSFLPPGEIKIKKIKSLQFSGEEVSLRKDREGKLSLGISLPSGRVEGIFREG
                     DNLHFNFNLVSSNLPFQGEVYWDMKDGKVEGKVSSPYNLSFKAILSFTEENMVFSPLE
                     LWETEENRCLFRGEGKIKNDLSFLEGEGEIYLGEEKNFLDIAMDMSLSSPLWRGKFSL
                     WGNRMDIKGGVSFLERSVLAFNIDSGSSFEGLELKEEMQAITSWEKGNIEINILDTLV
                     KFNRSLDWEGEGNFQGVINKEGQEWKGHLTWRGGYLQMGDFLVSEPLLNVELGQNGKT
                     VLYGEGNWGGGKITLSGEVFSGHLSLQGDFQSIEVEHLVEGMDFPLKGWVSGEWKREE
                     DKSIFLSLKEGKLFWEDWCLGAIEKGEIEFSPSVLIFRDLYLRHGEGVILGNLEKKRE
                     SWEGEIEVKDYPVSYSLEEENIELSLNGNVVVKSEDLFLSLFSPFWKIGKMEGKEFSL
                     AGKVEDGGIEIEDFSCDWEEGHLFLKGKVDPYREVNLKGEIQNLEIPSYWDIEGNLEK
                     IFLSLSGSWDEVIFLIEGEGKNFFWQGEPWGDYFHLLLRGKAPLPQKGETSFLTDYLN
                     PQLLQEGTIEVRGINLEQLMGKAFSYPVSGMTDVVINLDTQKNLWFFYSERLSFVVQD
                     FHFQGKLEGNYDGEDLVVNELFFQELTGGLEVGGSLKWEGKTKTLVGEIKGKVDRSFS
                     IPYGVLQLRGEANIAIS"
     gene            complement(2482..4152)
                     /locus_tag="PKN31_04945"
     CDS             complement(2482..4152)
                     /locus_tag="PKN31_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOA99305.1"
                     /translation="MIAQFKRRRWFILILTCFLLGLSFPVESASISYFPFSQIKRGMK
                     AVGKTVFYGTEVEDFQLEVMDVVEGKKVEESYFVVKVTDKKLEEMGGISAGMSGSPIF
                     IRGKIAGALSYSWETKDNLVGVVTPIEAMLPLWEEDLWEEEAIQGEEVIQEEKPISFS
                     PLVPESTIFVLGLGERASSGVANKLRERFCLKEIFTVPVLSWGKKRTSENDSLQPGSA
                     IGIQLVRGDAEVMSIGTLTLRDEDRILALGHPFLHRGEANYFLSSVYVNFSLQGANLP
                     FKVGKVIKEVGIIDQDRSAGVAGKIGVMPEVSKIVIKVRNEGKEEREYSFEVVRDEDI
                     LVDMLPELVLDAIDRSIDSQMSGSANVNLNLEGEDFSWQEEFFWISDSDIASATSSGL
                     GEVFKTILNNPCRKLNLSEVSIEVDVISGIQHAWLTSLDLPKVIGRNKEMEGKVNLFL
                     WREGERGVSFPFLVPADFLPGAAEITVRGKSSGNLELETNKEEASFSSSLYDYLQKRL
                     DNLHSEGLVVEIFSKAGSFPQDEKVYFTQWVGLPLILEGSVSEEVWIR"
     gene            complement(4159..5172)
                     /locus_tag="PKN31_04950"
     CDS             complement(4159..5172)
                     /locus_tag="PKN31_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOA99306.1"
                     /translation="MWNKRLGIDLGTATTLIYVYGKGIVLNEPSVVAISKNTNEILAI
                     GREAWEMIGKTPEYIVAHRPLREGVISDYEVTRRMLTYFIQQVCGKSLFKPDVVVCTP
                     SGGTGVEKRAVLDAAYRAGARRAYLIEEPMAAALGAGIDITGSSGSMVIDIGGGTTDI
                     AVLSLGGVVASQSVRVAGDQLDEAITKHLRKKFNLLIGEKTAEVIKIEIGWAVPPKEE
                     KTLKVKGRDLVSGLPKEISLTSSEIYKCLSEQLSSIIEAARSVLEKTPPEIAADIGEK
                     GICLTGGGSLLRGIDEMLSKALGTPVYVAEDPISCVALGAGKVLDYLKLLRDGLLYTK
                     REK"
     gene            complement(5190..6449)
                     /gene="murA"
                     /locus_tag="PKN31_04955"
     CDS             complement(5190..6449)
                     /gene="murA"
                     /locus_tag="PKN31_04955"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOA99307.1"
                     /translation="MSKFVIRGSKPLQGEVQIGGAKNAALPILAATLLADSPSLIKNV
                     PHLLDVETMFSVLESLGCKVEKVGEHAYRISPALYNTSEAPYELIRKMRASFLVTGPL
                     IARHGRAQVPLPGGCAIGSRPIDLHLKGFSHLGVEISMHSGYVEAWASQLHEGEVYFD
                     FPSVGATENVMMLAATIEGETVISNAACEPEVVDLANFISLMGAEIEGAGTDTIVVRG
                     TKNLHGCEYTIINDRIEAGTFCVAAAITRGRVEIKGVEYSSLRSVITKLEEIGAVVER
                     KGEDSIFVISENRPKAANIKTMPYPGFPTDMQAQFMALLSLAEGVSVITETVFESRFA
                     HVGELERMGAKIRVEGRSAVVTGVEKLAGTQVTAPDLRAGAALVLAGLAAEGETEVYG
                     VLHIDRGYENLETKLRSLGASIDRCDE"
     gene            complement(6568..8676)
                     /locus_tag="PKN31_04960"
     CDS             complement(6568..8676)
                     /locus_tag="PKN31_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HOA99308.1"
                     /translation="MGKKLIITEKPGVAQDIARALGKMKKKKEYWENEDYIVTWAVGH
                     LVTLAEPEDYDKKLKMWRLALLPVVPDEFKLKAIEGAEERLEVIKKLINSKEVEVIIN
                     ACDAGREGELIFRYIYEFLAGSKPVYRLWLSSMTQEAIKEAFSHLKPGDDYELLAQAA
                     KCRSEGDWLVGINGTRVFTARYKTLLSVGRVQTPTLAILVEREREIEKFVPVPYWEIF
                     AEFSSPSGSYRGKWEKKEDRVWEKEKAQSIVNKVKGQEGKVAEYKERKVKEPHPLLYD
                     LTELQRDANKLFGYSAKRTLDSAQRLYERHKLITYPRTNSRYLSPDLISQVEKCWRML
                     AECDFAEWVKEEFPRGKALKDKRIFDKSKVSDHHAIIPTGEKIDWESLSENDRRIMNL
                     IVKRFIASFYPEAVWVHRSIKTVVEDEKFVSKSKVLLEAGWRAIYGKEEGEEEDFLPA
                     VEKGTTVKTEKAWMEEKETKAPPRYTEATLLSAMEGAGRFVEDEELQEILKEGGIGTP
                     ATRAAIIERLIQVGYVERSGKTLIPTPKGKELINLIENIPVPELSSPQLTGEWEKKLN
                     QIEKGEFSRDTFMEEIRKFTVEMVEKVKSMEDRGEREKMSMPVGNCPLCGAPVHENRK
                     SFSCSRWKEGCPFTIWKKIAGRFINRQEAQKLITTGRTDKLYGFKSRKGKKFSAYLVL
                     QEEGKVEFEFSTREASSGVS"
     gene            complement(8691..>9304)
                     /locus_tag="PKN31_04965"
     CDS             complement(8691..>9304)
                     /locus_tag="PKN31_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99309.1"
                     /translation="DMPIDLILHTPGGLVLAAQQIACAIKRHPAKVTVFIPHYAMSGG
                     TLIALAADEIIMDPNAVLGPVDPQIGTVQAQYPAASILKALEEPNPNRDDQTLILGDI
                     ARKAINQVYQVVLNLVDERLPEEKARELARILSEGRWTHDFPITLEMAKELGLPVQEG
                     LPDEIYQLMDLYPQPQQQRPSVEYVPAPYAPHRRPTGGKEKVE"
BASE COUNT         2668 a         2349 c         1482 g         2805 t
ORIGIN      
        1 gaaatagcaa tattggcttc tcctctcaat tgtagcactc cataaggaat agaaaaggag
       61 cgatccactt tcccttttat ttctccaacc agcgtttttg tctttccttc ccattttaaa
      121 cttcctccta cctccaaacc ccctgtcaat tcctggaaaa acaactcgtt aacaactaaa
      181 tcttctccat cataatttcc ttccaatttt ccctgaaaat ggaaatcctg taccacgaaa
      241 gaaagtcttt ccgagtagaa aaaccacagg tttttctgag tatccaggtt aattactaca
      301 tcggtcattc ccgaaacagg ataggaaaaa gctttcccca ttagttgttc taagtttatc
      361 cctcttactt ctattgttcc ctcctgaaga agctgaggat ttaaatagtc agtaagaaat
      421 gatgtttcac ctttttgagg aagaggagct ttacctcgga gcaaaagatg aaaataatct
      481 ccccagggtt ctccctgcca aaaaaagttc ttgccctctc cttcaataag aaaaataact
      541 tcgtcccagg aaccagagag ggagaggaaa attttttcta aattcccttc tatatcccag
      601 taagaaggta tttccagatt ttgaatttct cccttgagat taacctctcg gtagggatca
      661 accttgcctt ttagaaagag gtgtccctcc tcccaatcac aggagaaatc ttctatttct
      721 attccaccat cttctacttt tcctgctaaa gaaaattctt taccttccat ctttcctatt
      781 ttccagaaag gagaaaaaag ggaaaggaac aagtcttcgc tttttaccac tacatttcca
      841 ttcagcgata attctatgtt ttcctcttcc aaagaataag aaacagggta atcttttacc
      901 tctatttctc cttcccaact ttccctcttc ttctctaaat tccccaggat aaccccctct
      961 ccatgccgga gatataaatc acgaaatatt agaacagaag gagaaaattc aatctctcct
     1021 ttctctattg cacccagaca ccaatcttcc caaaatagtt tcccttcttt cagagagagg
     1081 aaaattgatt tatcctcttc tcttttccac tccccagaga cccaaccctt taatggaaaa
     1141 tccattcctt ctacaaggtg ctccacttct atgctctgga aatcaccctg gagggaaaga
     1201 tgacccgaga aaacttctcc agagagagtt atttttcctc ctccccaatt tccttcacca
     1261 tacaataccg tttttccatt ttgccctaat tctacattta acaggggttc acttaccaaa
     1321 aaatcaccca tctgaagata accacccctc caagttagat gacctttcca ctcctgtccc
     1381 tctttattta ttaccccttg aaaatttccc tctccttccc aatccagaga cctattaaat
     1441 tttaccaaag tatcaagtat attaatttca atatttcctt tttcccaaga agttatagct
     1501 tgcatctctt cttttaattc taatccttcg aaagaagaac ctgaatctat attaaaagct
     1561 aaaacacttc tttctaagaa ggaaacccca ccctttatat ccattctatt tccccagaga
     1621 gaaaatttcc ccctccagag aggagatgaa agactcatat ccattgctat atccaaaaaa
     1681 ttcttttctt ctcccagata aatttcaccc tctccttcta ggaaagaaag atcgtttttt
     1741 attttccctt ctcctcgaaa aagacatctg ttttcttctg tctcccataa ctcaagagga
     1801 gaaaaaacca tattctcctc ggtaaaagaa agaatagctt taaaacttaa gttgtaaggg
     1861 gaagaaactt tcccttccac tttaccatct ttcatatccc agtatacttc cccctgaaag
     1921 ggtaaattac ttgataccag gttaaagtta aagtggaggt tatctccctc ccggaaaata
     1981 ccctccactc tcccggaagg aagagaaatt cccaaagata gtttaccctc tctatccttt
     2041 cttaaggaaa cctcttctcc agaaaactga agcgatttta tctttttgat ttttatctct
     2101 cccgggggaa gaaaagaaag agaaaaagaa ggtaattttt gaagaaattt ctgagaagaa
     2161 gacaattcta cttcttctaa gaacaaataa aaggctttct cagaaggtga agcaccaaag
     2221 aaataccgcc agtcccattg caaaatagct ctagaaacaa aaaaagagaa atcttctccc
     2281 ctcccactaa ctccctgcaa tcggagctgg ttaggaaagc gaaaggaaaa cttttcgata
     2341 tttatttcgc tttttacttt cttctccagt tcagaaggaa accggatagt aaagtaatca
     2401 attactcttt tttgaccata aaaccaaata aaacaaaaac tcaaaaaaaa acaaagccca
     2461 agcagagccc actttctcaa cttatctaat ccacacttcc tcagaaacac ttccttctaa
     2521 aatcagagga agacccaccc attgagtaaa gtaaactttc tcatcttgcg gaaaagaacc
     2581 tgctttgctg aatatctcta ctaccaaccc ttcgctatga aggttatcta atcgcttctg
     2641 caaataatca taaagagagg aagagaaact tgcttcttct ttattagtct ccaattccag
     2701 attaccactg cttttacctc tgacagtaat ctctgctgca ccaggtagaa aatctgctgg
     2761 tacgagaaaa ggaaagctaa cgcccctctc tccttcccgc cagagaaaca aatttacttt
     2821 tccttccatt tctttgttcc gtccaataac tttgggtaaa tctaaagaag taagccaggc
     2881 atgttgaata ccggagatta catctacttc tatactcacc tcacttaaat tgagctttcg
     2941 acaaggatta ttcaaaatag ttttgaatac ctctcctaag ccactggaag tggcgctagc
     3001 gatatctgaa tcgcttatcc aaaaaaactc ttcctgccag gaaaagtctt ctccttccag
     3061 gttaagattg acatttgcgc taccggacat ttgagaatct atactccgat caatagcgtc
     3121 taaaaccagc tcaggtagca tatctactaa tatatcttca tccctcacca cttcaaagct
     3181 gtattctctt tcctctttac cttcattcct gaccttaatt acaattttac ttacctctgg
     3241 cattactcct atcttgcctg ctaccccggc actccggtct tgatcaataa tccctacctc
     3301 ttttattacc tttcccactt tgaaaggtag gttggctcct tgcaagctga agtttacata
     3361 cactgaggaa agaaaataat tggcctcccc tcgatgcaaa aaggggtgtc ctaaagctaa
     3421 tatccgatct tcatcgcgta aggtaagagt cccaatactc attacttcag catctcccct
     3481 caccaattga atgcctatgg cacttccagg ttgcaaagaa tcattctctg aagttctttt
     3541 tttaccccac gaaagtacag gaacagtaaa aatctctttc aggcagaagc gctctctcaa
     3601 tttgttagct actccactac tagctctttc tcccaaaccc aaaacgaaaa tcgttgactc
     3661 aggaacaaga ggggaaaaag aaataggctt ttcctcctgg atcacctctt ccccctgaat
     3721 agcttcctcc tcccacaaat cttcctccca caaaggaagc atagcctcta tgggtgtcac
     3781 cactcctacc aggttatctt tggtttccca gctataagaa agagctccgg ctattttacc
     3841 ccggataaaa atcggagaac cactcatccc cgccgatatt ccacccattt cctcaagttt
     3901 tttgtctgtt accttaacca caaagtagct ttcttctact ttctttcctt ccactacatc
     3961 cattacttcc agctgaaaat cttcaacttc agtaccataa aaaacggtct tacctaccgc
     4021 cttcatgcct cttttaattt gagaaaaagg aaaataagag atagaagcgc tttctaccgg
     4081 gaaagatagc cccaggagaa aacaggtaag aatcagaata aaccagcgcc gtctcttaaa
     4141 ctgagcaatc aattctaatt acttctccct tttagtatag aggagtccat ctcttagcaa
     4201 tttcaggtaa tctaatactt tacccgctcc taaagctaca caggaaatgg ggtcttctgc
     4261 aacatatacc ggggtgccca aggctttgct gagcatctca tctattcctc gcaaaagaga
     4321 ccctcctccg gttaaacaga tacctttttc tccaatatca gcagctattt ctggaggggt
     4381 tttttccagc accgaccgag ctgcttctat tatagaggaa agctgttcac tcaaacattt
     4441 atatatttct gaagaagtaa gagagatttc ctttggcaaa ccagaaacta aatctctacc
     4501 cttaacttta agagtttttt cttctttagg aggaactgcc cagccaattt cgattttaat
     4561 aacctccgcc gttttttccc ctatcaggag gttaaatttc ttccgtaaat gtttagttat
     4621 agcctcatct aattgatctc cagcaactct taccgattga ctggctacca cccctcccag
     4681 ggaaagaaca gcaatatcag tagtacctcc accaatgtca atcaccatgc tccccgaaga
     4741 gccagtgata tctatacccg cacctaaagc agcagccata ggctcctcta taaggtaggc
     4801 tcttcttgct cccgctcgat atgcagcgtc cagtactgct cttttttcta ctcctgttcc
     4861 tcccgaagga gtgcacacca ccacatcagg tttaaagaga ctcttaccac atacctgttg
     4921 aataaagtaa gtgagcattc tccgagtaac ttcataatca ctaattactc cttcccgaag
     4981 aggacgatga gctactatat attcgggagt tttaccaatc atttcccagg cctctctacc
     5041 aatagctaaa atctcgttag tatttttgga tatagccacc actgatggct cgttcaaaac
     5101 tatacccttc ccgtaaacgt aaatcaaagt ggtagcagta cccagatcaa ttcctaatct
     5161 cttattccac ataaataatt cctcccgttt tattcatcac acctatctat agaagctcct
     5221 aaagagcgaa gcttggtttc taagttttca taacctcggt cgatatgtaa aactccatac
     5281 acttcagttt ctccttcggc agctagacca gccagaacca aggctgctcc ggctcgaaga
     5341 tcaggagcag taacttgagt tccagccaat ttttctaccc cagtaaccac tgcacttctt
     5401 ccttccactc gaattttagc ccccattctt tccagttctc ccacgtgagc aaaacgactc
     5461 tcaaaaactg tttcagtaat cacacttacg ccttctgcta aagaaagaag agccataaac
     5521 tgagcctgca tatcagtagg aaaaccagga taaggcatag ttttaatatt agcggcctta
     5581 ggtctattct ctgagatcac aaagatgctg tcttcccctt ttctttctac caccgctcca
     5641 atctcttcta atttagtaat taccgagcga agagaagagt attctactcc ttttatctct
     5701 accctacccc gggtgatagc tgccgctaca caaaaagtac ctgcttcaat tctatcatta
     5761 ataatagtat attcacaccc atgcaaattt ttagtacctc tcacgactat agtatcagtg
     5821 cctgctcctt cgatttctgc tcccattaaa gaaatgaaat tagccagatc caccacttct
     5881 ggctcacaag ctgcattaga tataaccgtt tctccctcga tagtagcagc cagcatcata
     5941 acattttccg ttgctcctac actgggaaaa tcgaagtaaa cttctccttc atggagctga
     6001 gatgcccatg cttctacgta accagagtgc atggatattt ctactcccaa atggctaaaa
     6061 cctttaagat gaaggtcaat agggcgagaa ccaatagcac accctccagg aagaggaact
     6121 tgagcgcggc catgacgggc aataagagga ccggtcacca ggaaagaagc tctcatttta
     6181 cgaatcagtt cataaggagc ttcactggta ttgtataaag caggagaaat ccggtaggca
     6241 tgttctccca ccttttccac tttacaccct aaactttcca gaactgagaa catggtctct
     6301 acatctaaaa gatggggaac atttttaatt aaagaaggag aatcagctaa taaggttgct
     6361 gctaaaatgg gtaaagcggc gtttttagct cctcctatct gtacttctcc ttgtaaaggt
     6421 tttgagcctc ggattacaaa cttactcaac tgttctctct ttccgcctct cgtataatta
     6481 gtgaaagcat caaatcaatt cctgcactat tataatcgat ttttttgaca tctacatcct
     6541 ggtttcctaa aaaacttctt aaaagtgtta ggaaacaccg gaggaagctt ctcgggtaga
     6601 aaattcaaat tctaccttcc cttcttcctg aagaaccaga taagcagaaa acttcttgcc
     6661 tttacgggac ttaaagccgt aaagcttgtc cgttctaccc gtggtaatta atttttgagc
     6721 ttcctggcgg ttaataaatc ttcccgctat cttcttccaa atggtaaatg ggcaaccttc
     6781 tttccatcgg gagcagctaa aagatttgcg attttcatgg actggagctc cacaaagggg
     6841 acagttccca acaggcatag acatcttttc tctttctccc cggtcctcca tacttttaac
     6901 tttttctacc atttccactg taaattttct aatttcctcc atgaaggtat ctcgagagaa
     6961 ttcccctttc tcaatctggt tgagcttttt ttcccattct ccggtaagtt gaggagatga
     7021 tagctcggga actggtatat tttctatcaa atttattaac tctttccctt tgggagtggg
     7081 tataagggtt ttaccactcc tttccacata acctacttgt attaatcgtt caattatggc
     7141 tgcccgggtg gctggagtac caatacctcc ctctttcaat atttcctgta gctcttcatc
     7201 ctctacaaac cggcctgctc cctccatagc tgacaaaaga gtagcttcag tatagcgagg
     7261 aggggctttg gtttcttttt cttccatcca ggctttttcc gttttcactg tagttccctt
     7321 ttcaactgcg ggtaagaagt cttcttcctc tccctcttct tttccataaa ttgccctcca
     7381 acctgcctcc agaagaactt tagatttgct aacaaatttc tcatcctcca ccacagtttt
     7441 gatactacga tgaacccaaa ctgcttcagg ataaaaagag gcaataaatc tttttacgat
     7501 taaattcatg attctcctgt cattttcact taaactttcc caatcaattt tctccccggt
     7561 ggggataatg gcatggtggt cggaaacttt gcttttgtca aatattcttt tatcctttag
     7621 agctttccct cgaggaaatt cttcctttac ccattctgca aaatcacact ctgctaacat
     7681 acgccagcac ttctctacct gggaaattaa atcaggagaa agatagcgag agttggtgcg
     7741 agggtaagtt atcagtttat gacgctcata gagtctttga gcactatcca gagtgcgttt
     7801 agctgaataa ccaaaaagtt tattggcatc tctttgtaat tcagtaaggt catagagaag
     7861 agggtgaggt tctttaacct tacgctcctt gtattcagct actttcccct cttgaccttt
     7921 aactttattt actattgatt gagctttctc cttctcccaa actcggtctt cctttttttc
     7981 ccatttcccc cggtaagaac cagaggggga agagaattca gcgaatattt cccagtaagg
     8041 aacgggaaca aatttttcta tttctctttc tctttcaacc aaaatagcca gagtaggagt
     8101 ttgcactctt cctacagaaa gcagagtttt atagcgagca gtaaacactc gagtgccatt
     8161 gatgcctacc aaccaatccc cctctgaacg acacttggct gcctgagcta aaagttcata
     8221 atcatctccg ggtttaagat gggaaaaagc ctcttttata gcctcttgag tcatagaaga
     8281 aagccaaagg cggtaaactg gtttgctacc cgctaaaaat tcataaatat agcggaagat
     8341 tagctctcct tctctacctg catcacaagc atttattatc acttccactt ctttagaatt
     8401 aatgagtttt ttgataacct ctaacctttc ttctgctcct tctatggctt tcagcttaaa
     8461 ttcatccggc acaaccggta acagagcaag acgccacatt ttcagttttt tatcataatc
     8521 ttctggttca gctaaagtca ctaagtgtcc aactgcccat gtaacaatat aatcttcatt
     8581 ctcccagtat tccttcttct tcttcatttt acccaaagca cgggctatat cctgagccac
     8641 accaggtttc tcagtaatga ttaacttctt acccataata ttcacatcct ttactcaact
     8701 ttttcttttc ctcctgtggg tcgacggtga ggagcataag gagcaggaac atactccacg
     8761 gaaggccgtt gctgttgtgg ttgaggataa aggtccataa gctggtaaat ctcgtctggt
     8821 aaaccctctt gcactggaag tcctaattct tttgccatct ctaaagtaat gggaaaatcg
     8881 tgagtccatc gaccctcgga caaaatcctg gctaactccc gggctttttc ctcgggaaga
     8941 cgttcatcaa ctaaatttaa aactacctga taaacctgat taatagcttt gcgagcaata
     9001 tcacctaaaa ttaaggtttg atcatcccga ttggggttag gctcttccag agcttttaaa
     9061 atcgaggcag ccgggtattg agcttgaact gtacctattt gaggatcgac aggtcctaaa
     9121 acagcattgg gatccataat tatctcatct gctgccagag caatcagcgt tcctcccgac
     9181 atagcgtaat gaggaataaa cactgtaact ttagccgggt gcctttttat agcacaagca
     9241 atttgttgag cagcaagcac cagccctccg ggagtgtgga gaatcaaatc aatgggcata
     9301 tcct
//
LOCUS       DAOPSS010000062         9025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6441_length_9025_cov_9.217503, whole genome shotgun sequence.
ACCESSION   DAOPSS010000062 DAOPSS010000000
VERSION     DAOPSS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6441_length_9025_cov_9.217503"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="floA"
                     /locus_tag="PKN31_04970"
     CDS             complement(<1..939)
                     /gene="floA"
                     /locus_tag="PKN31_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890719.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOA99310.1"
                     /translation="MQFLNYLVPLIIVIVALVIFFYLVPVGLWISALAAGVKVSITTL
                     IGMRLRRVEPASIILPLVKATKAGLDLDVGLLEAHYLAGGNVDRVVNALIAAERAGIP
                     LSFERACAIDLAGRNVLEAVQMSVLPRVIETPEVTAVAKDGIELKVKARVTVRADIDR
                     LVGGATEATVIARVGEGIVTTIGSSASHKEVLENPDSISKTVLEKGLDAGTAFEILSI
                     DIADIDVGQNIGARLQGDQAEADKRVAQAKAEERRAMAVAREQEMKAYTQEMRAKVVE
                     AEAEIPRAIAQALREGKLGVFDYYSLQNLQADTQMRK"
     gene            complement(943..2298)
                     /locus_tag="PKN31_04975"
     CDS             complement(943..2298)
                     /locus_tag="PKN31_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOA99311.1"
                     /translation="MRKICKCWLVGMMLLLFLMTGLSFPQSEEQVIFWVPVKDVPDLG
                     MVDWGLASFVKRALREAEDAGAQAVILEIDTSGGWVDAMLFIKDAIISSSCPTVAFIN
                     HRAWSAGAFIALACETIVFAPDATMGAAEPRSGTPEEIDKPDPKTVSAIRAQIEALAE
                     ARGRDPLVFASMVDRNVEIDGLVKKGELLTLRASQALEYGAGDLIAANREELLSKLGL
                     EGTVRELSPSWSENLARLVTNPVVVPILLTLALVGILMEILTPGFGVPGIVGLVSFSL
                     FFGGRYLAGLAGWEPLIFFLAGATLLIVELFLPGFGIAGISGMGALALSIYLVLRETS
                     ILFWQEAVYQLFFYVALMGGVFLVLLAFLPQSPVWQRLGLKKEITGKVYQIEEKSSEP
                     SVVGKTGIAKSMLRPVGIVEIEGKRWDAITEGEFISPGTKVRVEGVRGNTIVVKSEEG
                     G"
     gene            complement(2402..3358)
                     /locus_tag="PKN31_04980"
     CDS             complement(2402..3358)
                     /locus_tag="PKN31_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOA99312.1"
                     /translation="MKLPERASQPTMYFIGVTTHQSSIMKLFPLWAKELGLNNAVLKG
                     IDIEIHAPREVYREVVSFIKDDPLSLGALVTTHKIDLYEAAKDLFDYLDPYAQLFGEL
                     SSISKKDGRLEGHAKDPISCGLAMEAFIPPDFWKEHGGEVFIMGAGGSARAIAAYLFD
                     PKKEGNMPSKLYVSNRSTPRLYGMEEIMRKINPHINAEYVHSPQPEINDQVLKRVKPY
                     SLVVNATGLGKDRPGSPLTDNCEFPPHSLIWELNYRGDLKFIHQALAQKEKKNLKIED
                     GWIYFLHGWTQVIAQVFHIEIGKELFQRIEEISQDFRPPRLY"
     gene            complement(3360..3923)
                     /locus_tag="PKN31_04985"
     CDS             complement(3360..3923)
                     /locus_tag="PKN31_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HOA99313.1"
                     /translation="MKPFNVVIDFSTGELSPGKKTVRRVSDVKNMFLDREKAEEMIKN
                     GDPLVYEVIYADIPEEAGHLGHCTTIIYPGKVGKEFFLTKGHLHEQLGTAEIYLCLRG
                     KGKLLMESTDGDWAALDMFPGSSSYIPPFWSHRTVNVGQEPLIFYCIFPADAGHDYAT
                     IEEKGFPKLVVEEEGEVVLIDNPKRSR"
     gene            complement(3956..4981)
                     /locus_tag="PKN31_04990"
     CDS             complement(3956..4981)
                     /locus_tag="PKN31_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOA99314.1"
                     /translation="MKAAVFSYPPGIKVIEVPEPQPGEGEVLIRVKAVALCGTDLRIA
                     RRGHADISEGEKRILGHEVVGVIEEVGKGVDDLAPGMKVAIAPNYGCGKCYYCIQGLT
                     HHCPEGKALGITENGGFTEFMVVPQKAVSQGNVFPLLGETDLVSIAFVEALACVVHGF
                     LPLGVGFRDRVLIYGAGPIGLMFLELAQLQGAKEVWMADLSEDRLKVAQERGAKTIVV
                     GKEKVKERIKEAEVVVVAAPSPDAQKEALEIAGIYGRINFFGGLPPQVKEVALPSNLI
                     HYKELVVTGTTKSNNFHFRSALDLLLQEQLDLSYLVSHVLPLERIEEGLSLMERQQSL
                     KVVIVPE"
     gene            complement(5008..6543)
                     /locus_tag="PKN31_04995"
     CDS             complement(5008..6543)
                     /locus_tag="PKN31_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOA99315.1"
                     /translation="MVDLHYLIGSDLGTTATKTGLFDEEGNLIALARQKSNLIYGKDG
                     SVVQDPYEMLSSVVETVKEVVEKSGVSPSSILAIALDGQMAGIMGVDESGEAATPYDS
                     WLDVRCAPYTELMREKAEELIIKRSGMAPSINHGPKILWWKKEQPEVYQKIDKFTVPS
                     CWVSQKLCGLRGEETFIDYTYLHFSCFSHLEDGCWDRELMEIFEVEESKFPLIVKPWQ
                     VVGKIKKEWAEKMGIPAGTPVVAGCGDQAANNLGAGVVERGTAFDVAGTASCFSLSVD
                     RFVPDLEHKTILFPRAVLEGYYYPMAYINGGGLDLEWAKDEFFREWAELPNAFQLIDE
                     EVEKIVSSPSSLVFLPHLRGRNCPGEPYFRGVFAGFSWDHRREHLFRAILEGIAFEYA
                     YYLSIIRKLLPEIDFQEVRVIGGGAKSSFWNRIKASILRIPYVELDREEFAIWGSALL
                     AGFGVGLFSDLKEKSIHSVQKIKTHFPQEEWSAFYDNLFPFYLDTLEKMGKVFDSHKE
                     LFS"
     gene            complement(6537..7562)
                     /locus_tag="PKN31_05000"
     CDS             complement(6537..7562)
                     /locus_tag="PKN31_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA99316.1"
                     /translation="MNKLRFVLVGAGRAGMVHGQNLVHYIPEGELKAVVDVDLKRAEE
                     TSASLGVKSFYSTLDEALEKEDCDAVCIASLTFTHREMIEKAAGAKKHIFCEKPLAQS
                     LEEAEKIQEAVEKAGVKFQIGFMRRYDPGIRRAWEMVREGKIGDIVLIKSTGRGPGLP
                     APWIFDREKSGGMLAEVSSHDIDSVLWFVDKKPHHLHLEAKNFKGQEIREEFPDFYDH
                     YVLTMSFVEGPLGMIDGGCPVGYAYDARIEILGTEGMIKVGETEARGPVLYTSDKKSW
                     RDNQFSWRDRFREGYLEEMKSFIRSVLEDQNPSPSIIDGKRVVKVVELAHKSLGQQSP
                     VLWEGEW"
     gene            complement(7773..8516)
                     /locus_tag="PKN31_05005"
     CDS             complement(7773..8516)
                     /locus_tag="PKN31_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOA99317.1"
                     /translation="MEQKLNRKIPVPLYYQLKEIIKEEIEEGKLKPGDPIPSERELSE
                     KYGISRPTVRQALKELVYEGWLTREKGRGTFISKPKIDYGFIQHFTTFYDDMVEKGYS
                     LRTVILNKEIRKAGKEIARFLQIGEDEEIIFIDRLRFIEDEPIVRVNNHIPLALCPAL
                     VEEDLTDQSLYRIMTEKYGLKPYRAEISLEPIVANEVDSELLDIEKGAPMLLMENVTF
                     TEEGQIIDFFRSRFRGDKGKVRVNVYKER"
     gene            8847..8922
                     /locus_tag="PKN31_05010"
     tRNA            8847..8922
                     /locus_tag="PKN31_05010"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8880..8882,aa:Lys,seq:ttt)
BASE COUNT         2586 a         2310 c         1581 g         2548 t
ORIGIN      
        1 cttcctcatc tgggtatcag cctgcagatt ctggagagaa taataatcaa aaacccccaa
       61 tttcccctct cttaaagcct gagctatagc ccgaggaatc tctgcttctg cctccactac
      121 tttagctcgc atctcttggg tataagcctt catctcctgt tctctggcca cagccatagc
      181 tcttctttct tctgctttgg cctgagccac tcttttatct gcctcagctt gatccccttg
      241 cagacgagct cctatattct gacctacgtc tatatcagca atatcgatag agagaatttc
      301 aaaagcggtt ccggcatcta accccttctc tagaactgtt ttagaaatac tatcagggtt
      361 ctccagaact tccttgtgag acgcagaaga gccaatggtg gtaactattc cttctcccac
      421 ccgagcaatt actgtagcct cagtagcacc tcctaccagc cgatcgatgt cagctctaac
      481 tgtgacccga gcttttactt tgagctcaat tccatcttta gccaccgctg taacttcagg
      541 agtctcaatc acccggggaa gaacactcat ctgaaccgcc tccagaacat tccttccggc
      601 aaggtcgata gcacaagccc gctcaaaaga aagtggaata cccgctctct ctgctgcaat
      661 caaagcattc accacccggt ctacattgcc ccccgccaga taatgagctt ccagcaatcc
      721 tacatccaga tccagaccag ctttggtagc tttaaccaaa gggagaataa tggaggccgg
      781 ctcaactctc cgaagtcgca taccgatcaa agtagtaata cttaccttca ctccggctgc
      841 tagagcagaa atccacaacc ccaccggaac taaataaaag aaaatcacca gagccactat
      901 tacaatgatc aaaggaacta aataatttaa aaactgcaat ttttaccctc cttcctcaga
      961 tttaactact atagtatttc ccctcactcc ttccactctc accttagtac cgggagagat
     1021 aaattctccc tcggttatag catcccaccg ttttccttca atctccacta ttccgacagg
     1081 acggagcatg cttttagcta ttcccgtctt ccctactacc gagggttcag agcttttttc
     1141 ttctatttga taaactttcc cggttatttc tttctttagc cccaatcttt gccatactgg
     1201 actttgggga agaaaagcaa gaagaaccag aaatactcca cccataaggg caacataaaa
     1261 aaataattgg taaacagctt cctgccagaa caaaatactg gtttctcgaa gaacaagata
     1321 aatggataga gctaatgctc ccatccccga aattcctgct attccaaagc caggtaggaa
     1381 aagctccaca atcaaaagag ttgctcccgc caagaagaaa atgagaggct cccaccccgc
     1441 taaaccggca agatagcgac ctccaaaaaa aagagaaaac gaaaccaggc caacaatacc
     1501 cgggactcca aaaccaggag ttaaaatttc cattaaaatt cctactaaag ccagagtaag
     1561 aagaatagga actaccaccg gattagttac caatcgagcc aggttctctg accaggaggg
     1621 agagagttca cgaactgttc cttctaaacc taatttactt aatagttctt cccgattagc
     1681 ggctattaaa tctccagccc catactccag ggcttgagaa gcccggagag ttagaagctc
     1741 tccctttttt accaatccat cgatttcaac attgcgatct accatagaag caaaaacaag
     1801 tggatctctt cctcgagcct cagccagggc ttcaatttga gctcgaatag ctgatacagt
     1861 tttggggtca ggtttatcta tctcttccgg agtaccacta cgtggctcag ctgctcccat
     1921 agtggcatca ggagcaaaca cgatggtttc acaggccagg gcaatgaagg ctcctgctga
     1981 ccaggctcgg tgattaatga acgccacagt aggacaggaa gaagaaatga tggcgtcttt
     2041 aataaaaagc atagcgtcca cccagccacc tgaggtatcg atttccagaa tcactgcctg
     2101 cgctccagca tcttctgctt cccgaagagc tcgtttcaca aaactggcca agccccaatc
     2161 taccattcct aagtctggaa cgtctttaac tggaacccag aatataacct gctcttcact
     2221 ctgaggaaag gataaaccgg tcatcaggaa aagaaggagc atcattccca ctaaccagca
     2281 tttacagatt tttcgcattc ttctctcccc cttttcctga tggatgaacc gcagattcat
     2341 tattaatgct aactgggaaa aagattcttt attcccggat taatgaccag agattatctc
     2401 ttcagtaaag tcggggggga cgaaaatcct gagagatttc ctcaatgcgc tggaaaagtt
     2461 ctttgccaat ttcaatgtgg aaaacttgcg ctattacctg ggtccagcca tgaaggaagt
     2521 atatccagcc atcttctatt ttcaagttct ttttttcttt ttgagctaaa gcctggtgaa
     2581 tgaattttaa atctccccga taattgagtt cccatattaa actatgagga ggaaattcac
     2641 aattatcagt aagaggagaa ccaggacgat ctttacccaa gcccgtagca ttaaccacca
     2701 aagaataagg cttaactctt ttcaaaacct gatcgtttat ttccggttga ggagaatgaa
     2761 cgtattcagc attaatatgg ggattaattt ttctcataat ttcttccata ccataaaggc
     2821 gaggtgtgct acggttagaa acataaagtt tgctgggcat atttccctct tttttaggat
     2881 caaagagata tgcagcaatg gctcgagcgc taccccccgc tcccataatg aaaacttctc
     2941 ccccatgttc tttccaaaaa tcagggggga taaaagcttc catagccaaa ccacaggata
     3001 tagggtcttt ggcgtgaccc tccagccgcc catctttttt agaaatagaa gataattctc
     3061 caaacagctg agcataagga tctaaatagt caaacaggtc tttagctgcc tcataaaggt
     3121 caattttgtg agtggtgacc agagctccca gggagagagg atcatcctta atgaagctta
     3181 ccacctctcg ataaacttcc cgaggagcat gtatttcaat gtcaatcccc ttgagcaccg
     3241 cattatttaa acccaactcc ttagcccaca ggggaaaaag cttcataata gaggactgat
     3301 gagtggtaac tccaataaaa tacatggtag gctgagaagc gcgttctggt aacttcattt
     3361 tacctgctcc ttttggggtt atctatcaat actacctctc cttcttcttc caccaccagt
     3421 ttgggaaacc ctttttcttc gatggtcgcg taatcatgac cggcatcagc agggaaaatg
     3481 cagtaaaaaa tgagaggctc ctgccctacg tttactgtac ggtgagacca aaaaggagga
     3541 atgtaagaag aagaaccagg aaacatatct aaagcagccc agtctccatc agtagactcc
     3601 ataagcaact tcccttttcc tcgtaaacaa agataaatct ctgccgttcc cagttgctcg
     3661 tgaaggtgcc ctttagttag aaagaattcc tttcctactt ttcccggata aataatggta
     3721 gtacagtgac ctaaatgtcc cgcttcttcc gggatatcgg cataaattac ttcataaacc
     3781 agagggtcac cgttttttat catttcctcc gctttttccc tatctaggaa catattctta
     3841 acatcgctca cccggcgaac agtctttttc cccggggaca attccccggt agaaaaatct
     3901 attaccacat taaaaggctt cataatatca ccccttctat ttttattttt tgttttcatt
     3961 ctggaactat aactacttta agagactgtt gtctctccat aagagataag ccttcttcta
     4021 ttctctccaa gggtaaaacg tgacttacaa ggtaagaaag gtctaattgc tcttgcaaga
     4081 gcaaatccag agcgctccga aagtgaaagt tgttagattt ggtagtcccg gttactacca
     4141 attctttgta gtgaattaaa ttactgggaa gggcaacttc ttttacctga ggaggtaaac
     4201 caccaaagaa attaattctt ccataaattc cagctatctc taaagcttct ttttgagcgt
     4261 caggagaagg agcagctact accaccactt ccgcttcctt tattctctct tttactttct
     4321 ccttccccac cactatggtt ttagctcctc tttcttgggc tacctttagg cggtcttcac
     4381 ttaagtctgc catccagact tcttttgctc cctgcagttg agctaattct aaaaacatta
     4441 acccaatggg gccggctcca tatataagaa ctcgatctcg aaagcccacc ccgagaggaa
     4501 gaaaaccatg cactacacaa gctaaagctt ccacaaaagc aatagagacc aaatcagttt
     4561 ccccgagcag gggaaaaaca tttccctgag atactgcttt ttgaggaact accataaact
     4621 cggtaaaccc gccattttcc gttattccca gagctttccc ttccggacaa tgatgagtaa
     4681 gaccctgaat acagtaataa cactttccac atccgtaatt gggagctata gccactttca
     4741 tgcccggggc caggtcatct actccttttc ctacctcttc aatcacccct accacttcat
     4801 gtcctaaaat acgcttttct ccttccgaaa tatcagcatg tccccgccga gcgatacgca
     4861 aatctgtgcc acacaaagcc accgctttca cccggattaa aacctctcct tccccaggtt
     4921 ggggctcagg aacctctatc acctttatcc caggaggata agaaaatact gctgctttca
     4981 aggttgatca cgtcctttta atttgtttca gctaaaaagc tctttatgag aatcgaaaac
     5041 tttacccatc ttttccagag tatcaagata aaaagggaaa agattatcgt aaaaagcact
     5101 ccattcctct tgaggaaaat gagttttaat tttttgtact gaatggatag atttttcttt
     5161 gaggtcagaa aataatccca ctccaaaacc agccagtaac gctgaccccc agatggcaaa
     5221 ctcttctcta tctaactcca cataaggtat acgcaggata ctagctttta tccgattcca
     5281 gaaagatgac ttagcccctc ctcctattac tcgcacttcc tggaaatcta tctccggtag
     5341 gagctttcta attatagaaa ggtaataggc atattcgaaa gctatcccct caagtatggc
     5401 tcgaaacaga tgttccctac ggtggtccca gctaaaaccg gcaaatactc cccggaaata
     5461 gggctcgcca ggacagtttc taccccgaag atgagggaga aaaaccagag aggaaggaga
     5521 agaaacaatt ttctccacct cttcatcaat caactgaaaa gcattaggca actctgccca
     5581 ttctcgaaaa aattcatctt tagcccactc taagtctaat cctcctccat ttatataagc
     5641 catggggtaa taatatccct ctaataccgc tcgaggaaaa agaatagttt tgtgctctaa
     5701 atcaggaaca aatctatcta ctgaaaggct aaaacaagaa gcggtgccgg ctacatcaaa
     5761 agctgtaccc ctttctacca ctccagcgcc caaattatta gctgcctggt ctccacaacc
     5821 agcaaccaca ggagtaccgg cagggattcc catcttttct gcccactctt ttttaatctt
     5881 tcccaccact tgccaaggtt taactatcag aggaaactta ctttcttcca cttcaaaaat
     5941 ttccatcaac tctctatccc agcaaccatc ttccaaatga gagaagcagg aaaaatgaag
     6001 ataagtatag tctataaacg tttcctcacc ccggagacca catagcttct ggctgaccca
     6061 gcaagaagga acagtaaatt tgtcaatttt ctgataaact tctggttgct ctttcttcca
     6121 ccacaaaatt ttagggccat ggttaatgct gggtgccata cctgatcgct taataattaa
     6181 ctcttcagct ttttctctca ttagctccgt atagggagca catctcacat ctaaccagga
     6241 atcataaggg gtagctgctt ctccactttc atctactccc ataatccctg ccatttgacc
     6301 atcaagagca atagcgagaa tagaagaggg agaaactccg cttttttcta ctacctcttt
     6361 tacagtctcc accaccgagc tcaacatctc atagggatcc tgaacaacac ttccgtcttt
     6421 accatagatc aaattagatt tttgccgagc aagtgctatt aaatttcctt cttcatcgaa
     6481 aaggccggtt ttggtagccg tagttcctaa atcactacca atgagataat gcaaatctac
     6541 cactctcctt cccagagaac tggactttgc tgaccgagag acttatgagc aagctccacc
     6601 acctttacca ctctcttacc atcaataata gaaggagatg ggttttgatc ttctaaaaca
     6661 gaacggataa aagacttcat ttcctccagg tatccttccc gaaaacgatc ccgccagctg
     6721 aattgattat cccgccagga ttttttatcc gaagtataaa gaactggacc ccttgcttct
     6781 gtttctccta ctttaatcat tccttctgtt cctaaaatct ctatccgggc atcataggca
     6841 taacctaccg gacaccctcc atctatcatt cctaaaggcc cctcaacaaa actcatagtt
     6901 aaaacatagt gatcgtaaaa atcgggaaac tcttctctta tttcttgtcc tttgaaattc
     6961 ttagcttcca ggtggaggtg atgaggcttt ttgtcaacaa accacaaaac tgaatctata
     7021 tcatgagagg aaacttcagc taacatccct ccgctttttt ctctatcaaa aatccaggga
     7081 gcaggaaggc ccgggcctcg accagtagat ttaatgagaa ctatatctcc gattttcccc
     7141 tctctcacca tctcccatgc tctccgtatc cccgggtcat atcggcgcat aaaaccgatc
     7201 tggaacttta caccagcttt ttccacagct tcctgaattt tttctgcttc ctctaaactt
     7261 tgagccagag gtttttcaca gaaaatgtgc tttttagctc ctgccgcctt ttctatcatt
     7321 tcccgatgag taaaagttaa agaagcaata caaacggcgt cacaatcttc cttttccaga
     7381 gcctcatcca aagtggagta aaaagattta acccccaggc ttgcgctggt ctcttcggcc
     7441 ctctttaaat ctacgtctac tacagccttt agctctcctt cggggatgta atgaaccaaa
     7501 ttttggccat gaaccatacc tgctcttcct gctcctacca aaacaaaacg aagcttattc
     7561 aatgaagaat cactccttct caatttattt attagttaca attcttcctt ctttttcctt
     7621 caagctttaa gtatgcttca gctcctcctg gaaaaaaaca aataactata cctctgtaaa
     7681 ccacaatttt tactctatct atattaattc ctgatgctca agctgcttct cggttcccct
     7741 ttactgcata gagggattag ctaaaatgct ttttacctct ccttatatac gttcactctg
     7801 acctttcctt tatcccccct aaatcgggaa cggaagaaat cgattatttg cccttcttcg
     7861 gtaaaagtaa cattttccat gagaagcata ggggccccct tctcaatatc cagaagctca
     7921 gagtctactt cattggctac gatgggctcc aggcttattt ctgctcgata gggcttgaga
     7981 ccatattttt ccgtcatgat acgatagaga gattgatcgg ttaagtcttc ttccaccaac
     8041 gccgggcaga gagctaaagg gatatgatta ttaactctta ctattggctc atcttcaata
     8101 aatcgcagtc gatcgataaa aataatttcc tcgtcttccc ctatttggag aaaccgggca
     8161 atttcttttc ctgctttccg aatttcctta ttaaggataa cagtacgtag tgaataacct
     8221 ttttctacca tatcatcata aaaagtggta aagtgctgga taaaaccata atcaatcttc
     8281 ggcttagaga taaaagtccc tcttcctttt tctcttgtta gccagccctc atacactaat
     8341 tccttcaaag cctgtctaac tgtcgggcga ctgattccat atttttcaga aagttctctt
     8401 tccgaaggaa tcggatcccc aggcttgagc ttaccttcct caatttcttc ttttatgatt
     8461 tctttcagct ggtaataaag gggaaccggt atcttgcgat ttaatttctg ttccaaaata
     8521 atcacttctc ctccttaatt ttatatttta aagtaataat attaagtggt attaaccact
     8581 tgagaataga ttaagctttt tccatggaat tgtcaagaag tgggagagct gtttggaaaa
     8641 tccccatacc ctgattaaaa gaaggacgaa ataatgtttc ccgtattcta tatcgagact
     8701 caccaagtat ggaggttatg ccttaaatac gctgagaatt gcaaaatttt cttcacagag
     8761 ctttaaattc atcttctctt ctggtagaat aataacgctg ttgacaaaaa ggtagcttta
     8821 ggatatattg tcaagcgctg aagagtgggc cgttagctca gaaggtagag caccggactt
     8881 ttaatccggg tgtcgcaggt tcgattcctg cacggctcac cagagctcta cagagcctct
     8941 cttgcgactt ctccactttc tctaaaagca cgagtccgat gactataaat ctactcccag
     9001 aaagaggcga tttctgtggg gcagg
//
LOCUS       DAOPSS010000063         8943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6494_length_8943_cov_7.222660, whole genome shotgun sequence.
ACCESSION   DAOPSS010000063 DAOPSS010000000
VERSION     DAOPSS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6494_length_8943_cov_7.222660"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1007
                     /locus_tag="PKN31_05015"
     CDS             27..1007
                     /locus_tag="PKN31_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOA99318.1"
                     /translation="MRFGKKLLLALTVVLLSTLLLGVGLAQEEEVNVWVVVHGGIADP
                     FWKRVEKGVMDAATNHSDLEVTYTGPSEYNFTEFIADIEAAVASKPDVLVCTLTEPDA
                     MDEVLRTAIADGLPVIAINAPDLREPPENRIPVLTYVGEDSYHIGVVAAEETLKRFTP
                     KRALFVNHHPGARNIELRGQGFIDTLGKNDIPAEALDITADAVQGAEITLAYLVAHPD
                     TEVIFSTNTLRTEAIVTRLLDEGIDVGNEVKIASMDMSEKVLEYIQEGQVMFTLDQQQ
                     YLQGYLGVEFAYLHAKYGFTPPPAPVSTGPGVITAENVPQLMELSKQGYR"
     gene            1070..2092
                     /locus_tag="PKN31_05020"
     CDS             1070..2092
                     /locus_tag="PKN31_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99319.1"
                     /translation="MLIIQNQEIAHHQNEGIRSKWTFEELRKIPEFGALIAFLALFIF
                     FAICAPRFFSLRNITSLLTIIAELGIMTVGISFLMIGGEIDLSVSSAYATAGFVFVTL
                     ANIFTFPLGSLLAFIVALLVSLLIGFVNSRVVLRGGIPSFIATLGMMMFLRGILLGIT
                     GGRSISYAGDSLVPSLLTGIMAYRFRPSHFWFIAIVVVFSFILNHTVFGNRVFATGGK
                     KLAAYTMGVNVNQVKTACFMISSVLAGLSGVIAISRFSLANVSFGQGMELEAIAASVI
                     GGTLMTGGYGSIIGAALGAGIMGMVRTGLVMAGVPSYWYNAFVGIILVIAAVVNLKLR
                     STILGE"
     gene            2105..2866
                     /locus_tag="PKN31_05025"
     CDS             2105..2866
                     /locus_tag="PKN31_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA99320.1"
                     /translation="MNPKQPLVRMENIQKKFGKVRALKGVDFEVYDQEVVGLLGDNGA
                     GKSTLIKILSGVYRPDGGTIYFQGEKVDINSPEEARKLGIETVHQDLSLIEKMSVARN
                     FFLGKEPTKKIGPLEILDLKKMNRECMIGLKQIGIELRSPTEFVSTLSGGERQAIAIG
                     RAAYFGARILILDEPLRNLSIREQRNTLNHIQEARNKGSSVIFITHNVHHVCPVADRI
                     VLLENGEKLGEVKAGELTPEEIAECIATGQMIKVN"
     gene            3217..>3887
                     /locus_tag="PKN31_05030"
     CDS             3217..>3887
                     /locus_tag="PKN31_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOA99321.1"
                     /translation="MEEDIEEYSTERKDTLSKFREGMIASLPIFIGYIPVAVTFGLLA
                     KSTGLTLLETFLFSALVFAGASQFMALQLIQGGAEPIQIIVATFIMNFRHFLMSSHLS
                     AILPAKEKPWLLPFLSFGITDESFAYLSTSHASKEVDFILGLEYSAYVSWVGGTVTGY
                     LFGNILPPILQASMGLALYTLFIALLVPEAKRSLSVAITAVIGGLTHSCLAWLKIFSP
                     GWNIIL"
     assembly_gap    3888..3987
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4106..4414
                     /locus_tag="PKN31_05035"
     CDS             4106..4414
                     /locus_tag="PKN31_05035"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOA99322.1"
                     /translation="MNNIKIIIGMMIVTFIPRYLPLLAFSRLNIPKPLKKLLRYIFPA
                     ALGALILPGAITAIPNYPAAAAAGIATAIVCSWFREEISLTVFLSVIVTLISLYLFGY
                     "
     gene            complement(4506..5399)
                     /locus_tag="PKN31_05040"
     CDS             complement(4506..5399)
                     /locus_tag="PKN31_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOA99323.1"
                     /translation="MMSSVVKTSVQNKNYATMMKDMKKFTITGKITWKILGAVVWFFL
                     IFTKADAQSYDSLALYLSQNEDIPYYLAQAIAIYPHDPRAFTEGLVYENGFLYESTGL
                     HGESSLRKVNLSTGEIIHLVEIEDQYFGEGLTIVGSTLIQLTWKSRVALLYHKDTLEL
                     EKTLYYPYEGWGVTYDGSHLIVSDGSNLLRFLNPVDFSLIRELPVSSGGKLIDQLNEL
                     EYINGKIYANVWHTDIIVIIDSQDGEVVGWIDVSQLEEKRNTREDVPNGIAYDAENNR
                     LFVTGKRWSHVYEIQLPELIK"
     gene            5457..6485
                     /locus_tag="PKN31_05045"
     CDS             5457..6485
                     /locus_tag="PKN31_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOA99324.1"
                     /translation="MDYTHQEILRQPTTWGKTLNYLQEKESYFSQFLAETHDNVVFTG
                     CGSSYNLSLVASHFWQAGKQVPAKGVPASETFLFPGGVFLPQNQYLLFGISRSGETTE
                     TVKALQHFKDKFRGKTVGISCEENSSLLKVADYELALPFAREESVVMTQSFSSILLSL
                     LFLSLPPNKNIDNLSSLPEILEKYLPSGEKAIKDLASNLSFDKFIFLGNGPYHGVAWE
                     GSLKLKEMSVTPTETFHFLEFRHGPKSVVDEKTLIIALTSQRAFSWEKEVVMEMIDLG
                     ATILHLGKNSLNSSRAIEIIFPEESLDDFSLPVLYLPFLQLLGYYRAKNKGLNPDSPR
                     HLTKVVKI"
     gene            6482..7627
                     /gene="nagA"
                     /locus_tag="PKN31_05050"
     CDS             6482..7627
                     /gene="nagA"
                     /locus_tag="PKN31_05050"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOA99325.1"
                     /translation="MRWGIKGKVVTPGEVIPGMVVVRESRIEGIFPHPGNEDLEVVYD
                     FKDSFIFPGLVDLHLHGLQGEDVTEGGVKSIQKISALLPSFGVTSFLPTTLTADLATL
                     FKIIEETKSEIMASREGARVLGLHLEGPYLSLGRRGAHDPTYLRKPHEEEIKSLIQKG
                     EGVIKRITIAPELPGALEAIEYLAQNGILISLGHSEADYETSKRAWERGAKMVTHLFN
                     GMDPLHHRQPNLLAFALGNDEIYAEIIADKIHVKPEVIKIALKCKAPDHLCIVSDALK
                     VSNLPEGVYELSGQKVEVREGMAYLPSGTLAGSTFLLPQMLYNLRELFSISLPELAKM
                     ASFIPAQLVGKEKELGSIEKGKIADIVVFDNHFQVEAVFINGVMTRG"
     gene            7634..8617
                     /locus_tag="PKN31_05055"
     CDS             7634..8617
                     /locus_tag="PKN31_05055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA99326.1"
                     /translation="MYKLSRGSEVKNIVEELLYLHSLSFGAYPGVLPTSRDFLEWYIE
                     RPGLGLENILLFLYEDKVVSSLFLTLSHIYLEGELTPVGIIDTVMTHPDYRRRGLASA
                     LLKEAEKIMAEKECRMGYLYTVPESPQFYLYQRLGYVDFKRIFHLQREKGEKPFYDRE
                     GKESPHQRVREFLNQNLSSYNGFVYFSEEQWMWRKAKRPVCLPTKLISLQDENNHLLA
                     TLTITEGEITTDKDSEPIIYLSDWYGINQEAKEKVLVKSLRLVGEERKIDCLSSPDNR
                     EEWKILLRYNFRPVIPESAMLHPLDEDIQQKITTIDSKRPWYPLIESIVGV"
     gene            complement(8628..>8943)
                     /locus_tag="PKN31_05060"
     CDS             complement(8628..>8943)
                     /locus_tag="PKN31_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99327.1"
                     /translation="TLSGTIGTNSPDRVVPATRIFKGLAATAIFCVIQSWNEFALAFF
                     LTSFNARTVPTMVTFFLSVLGVIWGEMAAVGVIATLPVLIFAIIVQKYLVRGLTFGAI
                     KS"
BASE COUNT         2638 a         1763 c         1818 g         2624 t
ORIGIN      
        1 tttttaatta attggagggt taaaaaatga gatttggcaa aaagttactt ttagctttaa
       61 cggtagtttt gttatccact cttcttctgg gagttggttt agcccaagaa gaagaggtta
      121 acgtatgggt agtggtgcat ggagggatag ccgatccttt ttggaaacgg gtagaaaagg
      181 gagtaatgga tgcggctacc aaccattctg atctggaagt cacctacact ggcccctcgg
      241 aatacaattt caccgagttt atagctgata ttgaggctgc tgttgccagt aaaccagatg
      301 tcttagtgtg caccttaacc gaacctgacg caatggatga ggtattacgt actgctatcg
      361 ctgacggact cccggtaatt gccattaatg ctcctgactt acgcgaacca ccggaaaacc
      421 ggatcccggt cttaacctat gtgggagaag attcttacca tataggagtg gtagcagctg
      481 aagaaaccct aaaacgattt acccccaagc gagcactgtt tgtcaaccac catcccggag
      541 ctcgtaatat cgaattgaga ggtcagggat tcattgacac gctggggaaa aatgatattc
      601 cggccgaagc acttgatatt acagccgatg ccgttcaagg agctgaaatt accttagctt
      661 acctcgtcgc tcatcccgac accgaggtta tctttagcac taatactctc cgcactgaag
      721 ctattgtaac tcgacttcta gatgaaggaa tagatgtagg aaatgaagta aaaattgcct
      781 ccatggatat gtctgaaaaa gttctggagt atattcagga aggacaggta atgttcaccc
      841 ttgaccagca gcaatatttg caaggttatt taggagtaga atttgcttac cttcatgcta
      901 aatatggttt tactcctcca ccagcaccag tttctaccgg accaggagtt attactgctg
      961 agaatgtgcc tcaactaatg gagttatcaa aacaaggtta tcggtaagag acctaagtgg
     1021 cgtggcggga tcatctggtc ccgcccaatc tggctaggag gtaactacaa tgttaattat
     1081 acaaaaccag gaaatagcac atcatcaaaa tgaaggcatc cgttctaaat ggacttttga
     1141 agaattgagg aaaattccag aattcggggc attaatagcc tttttagccc tttttatttt
     1201 ttttgccatt tgtgctcctc gatttttttc tcttcgaaac atcacttctc ttttaaccat
     1261 tatagctgaa ttaggtatta tgacagtcgg tatatctttc ttaatgattg gaggagaaat
     1321 agatctttcc gtcagtagtg catacgccac agctggtttt gtctttgtta ctttagctaa
     1381 cattttcacc ttccctctcg gttccctgtt agccttcatc gttgccttgt tggtttccct
     1441 attaatagga tttgtcaaca gtcgagtagt attgagagga ggtattcctt cttttattgc
     1501 taccctgggg atgatgatgt tcttgcgggg gattcttctg ggaatcactg gcggacgctc
     1561 tatttcctat gctggagata gcttggttcc cagcttgtta accggaatta tggcctatag
     1621 atttagacca tcccatttct ggtttatagc tatagtagtc gttttttcct ttattctcaa
     1681 tcacaccgta tttggtaacc gggtatttgc cacgggggga aagaaattag ctgcctatac
     1741 tatgggagtt aatgtaaacc aggtaaaaac tgcctgcttt atgatttcat ctgtcttagc
     1801 cggtttatca ggggtgattg ccattagccg atttagcttg gctaacgtct cttttggcca
     1861 ggggatggag ctggaagcaa tcgcagcttc agtaattgga ggtactctta tgaccggagg
     1921 ctatggttca atcataggag cagctttagg tgcaggcatt atggggatgg tgcgaactgg
     1981 tttagttatg gcaggtgtcc ccagctattg gtacaacgcc tttgtgggaa taattttggt
     2041 tatagccgcc gtggtaaatt taaagctgag aagtaccatt ctgggagaat aaagaggtgg
     2101 agagatgaat ccgaaacagc cattagtaag aatggagaat attcaaaaaa agtttggaaa
     2161 ggttagagcc ctaaaggggg tagactttga ggtttacgac caggaagtag taggactttt
     2221 aggagacaat ggagcgggta aatctacttt aattaagatc ctgtccggag tctatcggcc
     2281 cgatggggga actatatatt tccagggtga gaaagtagat atcaattccc ctgaggaagc
     2341 ccgcaaactg gggattgaaa ctgttcacca ggacctctcc ttgatagaga aaatgagtgt
     2401 agctcgaaac tttttcctgg gcaaagaacc taccaaaaaa ataggccctc tggaaatcct
     2461 ggatttaaaa aagatgaatc gtgagtgtat gattggtctt aaacagatag gaattgagct
     2521 tcgctctccc accgaattcg tttcaactct ctctggaggc gaaagacaag ccattgccat
     2581 tggcagagcg gcttattttg gagccagaat tcttattctg gacgaacccc tacgtaatct
     2641 atctattcgg gaacaacgaa acacattaaa tcatattcaa gaagcccgga ataaagggtc
     2701 ttcggtcatt tttattactc acaatgttca tcacgtatgt ccagtggccg accggatagt
     2761 gctgttagaa aatggagaaa agctaggaga ggtaaaagca ggagaactga ccccggaaga
     2821 gattgccgaa tgcattgcta ctggccagat gataaaagtt aactaaaaag aagagcaaag
     2881 gatcaaggat acagtaagcg tcatagtaaa agcagtgccg cttcactact ttactgcttc
     2941 cttaacttat aatttttgag taacttttat agagttaatg ttccttttca tccacttcaa
     3001 tgctttctgg ccaatattct acccttcccg tggggatacc cacagcagtg aacgctttga
     3061 acccatactt attactttgc tatagtaaag taagatggaa aatagcaaat ttctcccttg
     3121 tattatcaaa agtgacaccc tattctttat ttttgagata taataatttt ctattattag
     3181 gaaaaattca gaaaatgcga taggtgaaca ggttatatgg aagaggatat agaagaatac
     3241 tccacagaac gaaaagatac actctcaaag tttagagaag ggatgattgc ttctctccct
     3301 atttttattg gatacatacc agtcgcagtg acttttgggt tattagcaaa atcaacaggc
     3361 ctgactctcc tggaaacatt tcttttttct gccttagtat ttgccggtgc cagtcagttt
     3421 atggctctgc agcttatcca aggaggtgca gagccaatcc aaatcattgt agcaaccttc
     3481 attatgaact tccgccattt tcttatgagt tcacatctat ctgctatttt gcccgcaaaa
     3541 gaaaagccat ggcttcttcc atttttaagt tttggcataa ctgatgagtc tttcgcatat
     3601 ctttctacct ctcatgcctc taaagaagtc gattttatcc taggccttga atattcagct
     3661 tatgtaagct gggtgggagg aactgtcacc ggttatttat ttggtaatat actacctcct
     3721 atccttcaag ccagtatggg attggcactt tacactcttt tcatcgctct tctcgttcct
     3781 gaagctaaaa gatctctctc ggtggcaata actgctgtaa taggaggatt aacgcattca
     3841 tgtcttgcct ggctcaaaat tttttctccg gggtggaata taattctnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnncgc attcatgtct tgcctggctc aaaatttttt
     4021 ctccggggtg gaatataatt ctagccattc taatagccgc aagcattgga gcttggataa
     4081 tagatgaaga tgaagaggag gaccaatgaa taacattaaa ataattatag gaatgatgat
     4141 agttactttt attccccgtt atcttccttt actggccttt tctcgactta atattcctaa
     4201 accgcttaag aaacttctcc gatatatttt tcctgctgct ctgggagcat tgatacttcc
     4261 gggggcaata actgctattc ctaattatcc tgctgcggcc gcagcaggta ttgctacagc
     4321 aatagtctgc tcctggttta gagaagaaat ttccctgact gtatttttgt cagtaatagt
     4381 cactttaatc agcttatacc tttttggcta ttgagtttat atagttaatc gcaaaagcct
     4441 tgtaatacta aggcttttac tccagtgtat ttttatgctt ttgtcttaca tagtaaactg
     4501 cttaactatt taatcaattc cgggagctgt atctcataaa catgagacca tctcttacca
     4561 gtcacaaata agcgattatt ttcagcatca taagcaatac cgtttggaac atcttccctt
     4621 gtatttctct tttcttcaag ctggctaaca tcgatccacc ccaccacttc accgtcctga
     4681 gaatctatta tgacaataat atcagtatgc cagacgttgg cataaatttt accgttaatg
     4741 tattctaact cattaagctg gtcaatgagt tttccccctg agctaacagg caattctctt
     4801 attaaagaaa agtccacagg attaagaaac cgcaacaaat tagaaccatc acttacaata
     4861 agatgacttc catcataagt aacaccccat ccctcataag ggtaatagag ggttttttcc
     4921 agttctaagg tatccttgtg ataaagtaag gcgactcttg acttccacgt tagttgaatt
     4981 aaagtagaac ccacgatagt aaggccctca ccaaaatatt ggtcttcaat ttctactaaa
     5041 tggatgattt ctccggtaga aaggttaacc ttccttaagc ttgattcacc atgtaaacca
     5101 gtactttcat ataaaaaacc attttcataa actaatcctt cagtaaaagc tcgagggtcg
     5161 tgaggataga tagctatcgc ttgagccaga taataaggga tatcttcatt ttgagacaga
     5221 tacaaagcaa gtgagtcata agactgagcg tcagcttttg tgaaaataag aaagaaccaa
     5281 acaacagccc ccaatatttt ccaggtaatt ttaccagtaa tagtaaattt tttcatatct
     5341 ttcatcattg tagcataatt tttattttga accgaagttt ttacaactga actcatcatc
     5401 aacaaaaaaa tgagatataa tggtcttgaa caagaaggat aaaaggagga ataattatgg
     5461 attacactca ccaggaaata ctccggcaac ccactacttg gggaaagacc ctaaattatc
     5521 tccaggagaa agaaagctat ttctctcaat ttttagcaga aacacatgat aatgtcgttt
     5581 ttacgggatg cggaagttct tacaacctct ctctcgttgc cagtcatttc tggcaagcag
     5641 gaaaacaggt tcctgctaag ggagtgcctg cttcggaaac ttttcttttt cccgggggag
     5701 tatttttgcc tcaaaaccag tatctcctct ttggtatttc tcgctcaggc gaaactacag
     5761 agacagtcaa ggctcttcag cacttcaaag ataagttcag agggaaaact gtagggataa
     5821 gttgcgaaga aaattcttct cttttaaaag tagccgatta tgagctggct cttcctttcg
     5881 ctcgagaaga aagtgtagtg atgacccaat ctttttcaag catcctgtta tctcttctct
     5941 ttttgtcttt accgccgaac aaaaatattg ataacctatc atctctccct gaaattttag
     6001 aaaaatatct cccatctgga gaaaaggcca taaaagactt agcatctaac ctttcctttg
     6061 ataagtttat atttttaggt aatggtcctt accacggtgt cgcctgggaa ggttccctaa
     6121 aactcaaaga gatgagtgtg actcccactg agacctttca ttttctggaa tttcgacatg
     6181 gtcctaaatc agtagtagat gaaaaaactt taatcattgc tctaacttca caaagagcct
     6241 tttcctggga aaaagaagta gtaatggaga tgatagattt gggagctacc attctccact
     6301 taggaaaaaa ctcccttaac tcttctcgcg caatagaaat aatatttccg gaagaatcct
     6361 tagatgattt ttctcttccg gtattatatt tgcctttctt acaacttctg ggctactacc
     6421 gagcaaaaaa taaaggatta aatcctgata gcccaagaca cctgaccaag gtagtgaaaa
     6481 tatgagatgg ggaattaaag gtaaagttgt cactcccgga gaagtaattc ctggaatggt
     6541 ggtggtgaga gaaagtcgta tcgaaggcat tttccctcat ccagggaatg aagacctgga
     6601 agtagtttat gactttaaag atagtttcat attccctggt cttgtggatt tgcacctcca
     6661 tggcctccaa ggagaggatg ttaccgaagg tggagtaaaa agtattcaga aaattagtgc
     6721 tcttctgccc tcttttggag ttacttcctt tcttcccacc actttaactg ctgatttagc
     6781 tactttattt aaaattattg aagaaactaa atctgagata atggcttcca gagagggagc
     6841 tcgagttctg ggccttcacc tcgaaggccc ttatctatca ttagggagga gaggagctca
     6901 tgaccctacc tatttaagaa aaccccacga agaagaaata aaatccttaa tccaaaaagg
     6961 agaaggggta attaaaagaa taaccatagc tcctgagctt cccggagcac ttgaagccat
     7021 cgaatattta gctcaaaacg gcattttgat ttccttagga cacagtgaag cagattatga
     7081 gacctcaaag cgagcctggg aaaggggagc taaaatggta actcaccttt tcaacggtat
     7141 ggaccctctt caccatcgcc aacccaatct cttagctttt gccctgggaa acgatgaaat
     7201 atatgccgaa attattgccg ataaaattca tgtaaaaccg gaagtaataa aaatagctct
     7261 gaaatgtaaa gcccccgacc acctttgcat agtaagcgat gctctgaaag taagtaactt
     7321 gccagaaggt gtgtatgaat taagtggaca aaaagtagaa gtaagggaag ggatggctta
     7381 cctaccttca ggaactttag ccggtagtac ctttcttctc cctcaaatgc tttataattt
     7441 gagagagtta ttctctattt ctttaccaga gttagctaaa atggccagtt ttattcctgc
     7501 tcagctggtg ggaaaagaaa aagaactggg tagtattgag aaaggcaaaa tagctgatat
     7561 cgtggtattc gataaccact ttcaagtaga agccgtattt ataaacggag taatgacaag
     7621 ggggtagaca cttatgtata aattgagtcg gggttcagag gtaaaaaata tcgtagaaga
     7681 acttttgtat ctccattctc tttcttttgg cgcttaccct ggagtgttac caactagcag
     7741 agatttttta gaatggtata tagaacgccc aggattaggt ttagaaaaca tcctcctttt
     7801 tctatatgaa gataaagtgg tatctagcct ctttcttact ctatcccaca tttatttgga
     7861 gggagagcta acaccagtgg gtattatcga tacagttatg actcaccctg attatcgtcg
     7921 aagaggttta gcttcggctc ttcttaaaga agcagagaaa atcatggcag aaaaagaatg
     7981 tcgaatgggc tatctctata ccgttcccga atctcctcaa ttttatctct accagagatt
     8041 gggatacgtt gatttcaaga ggatttttca tctacagcga gaaaaaggag aaaaaccttt
     8101 ctatgaccgg gaagggaaag aaagccctca tcaaagggtg agagaatttc taaatcaaaa
     8161 cttatcttcc tataatggct ttgtttactt ttccgaggaa caatggatgt ggcgaaaagc
     8221 gaaaagacct gtttgtctgc ctactaagtt aatatctttg caagatgaaa ataaccatct
     8281 tctggctact cttactatta ctgagggaga aattaccact gacaaagatt cagaacccat
     8341 catctattta agtgactggt acggtattaa ccaagaagct aaagaaaagg ttttagtaaa
     8401 aagcttaaga ttggtaggag aagagagaaa aatagattgt ttgtcctctc ccgataacag
     8461 ggaagagtgg aaaatactcc ttcggtataa cttccggcct gtcatccccg aatctgctat
     8521 gcttcatcct ttagatgaag atattcaaca aaaaataacc accatagata gcaagaggcc
     8581 ctggtacccc ctcatcgagt caattgttgg ggtatagtat tatagtatta gctttttatt
     8641 gctccaaaag ttagacctcg gaccagatac ttctgaacga ttatagcaaa aattaaaacc
     8701 ggtaaagtgg caataactcc tactgctgcc atttctcccc atatcactcc taaaactgag
     8761 agaaaaaagg ttaccattgt aggaacagtt cgagcattaa agctggtgag aaagaaggct
     8821 aaggcaaact cgttccaaga ttgaattaca caaaaaatgg cggtggcagc taaacccttg
     8881 aatattctgg ttgccggaac caccctgtcc ggagagttgg ttccaatggt tccggacaat
     8941 gtt
//
LOCUS       DAOPSS010000064         8865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6570_length_8865_cov_8.705902, whole genome shotgun sequence.
ACCESSION   DAOPSS010000064 DAOPSS010000000
VERSION     DAOPSS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6570_length_8865_cov_8.705902"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..846)
                     /locus_tag="PKN31_05065"
     CDS             complement(316..846)
                     /locus_tag="PKN31_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOA99328.1"
                     /translation="MKRTKALVYLGFLTTLSIVLTRLASIRIPLGGVEVIRIGFGQLP
                     VIMAGIYFGPGSGALVGGLSDFLGFFLNPMGPYLPHFTLAAILHGLLPGLLFHRSSQP
                     LRKRIFWSIAIPQIAVGIFLTSYFLHSIFGIPWNILLLPRLVSASLQIFIYFYLLLLL
                     SRSPFLSFAYLQDSHQ"
     gene            complement(871..1764)
                     /locus_tag="PKN31_05070"
     CDS             complement(871..1764)
                     /locus_tag="PKN31_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99329.1"
                     /translation="MLQATIRMATPLTFAALGGMFCERAGVVNIGLEGIMLNSAFWAV
                     FATHFSGSPGIGLLAGVLSGMALGAILALLTVKYYADQIVIGTGINIFGLGFSAFVCQ
                     KIWGSRGASGVVAGLPPLHFSFLDKIPYLGPLLNNNTILVYISFLAFILSHYFLFYTP
                     WGLRLRAVGENPQSADTAGINVHLYKMIFVTLGSTLAGLGGVFLSLAHLNFFAWGMTS
                     GRGFIALAAMIFGKWTPIGCFLASLLFGFADALQMRIQTLGILPPQIILLLPYLITIL
                     VLTGLVGKATPPSDYKPYIKK"
     gene            complement(1796..2842)
                     /locus_tag="PKN31_05075"
     CDS             complement(1796..2842)
                     /locus_tag="PKN31_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99330.1"
                     /translation="MKLLSKKSLSSFSILPEIIAILLAFLISALILLFSGYSPLVAFS
                     ALFRGAFGSKNAWGQTLTQATPILFTALAFLLTFRTGIFNIGVEGQFLMGAIASALAG
                     IYFSGLPAPLHIALSLLAGGVMGALWGLIPALLKAYLGVHEVITTMMMSYIASYLTSY
                     LVNYPFKAPGWVAQTEPVAKSALLPKILPPTQLSLSFIIALFFSFLVSFFLFHTYLGF
                     QVRAVGLNPEAAENKGIAIKNRQILAFLLSGFIAGIGGGGEILGVHGRFVDGFSPGYG
                     WDGIAVGLVGKLHPGGCILASILFGMLRAGGMTMTRVTKVPLDLSLIIQSLVIILVAA
                     PYFIKTVIMRRKDL"
     gene            complement(2826..4349)
                     /locus_tag="PKN31_05080"
     CDS             complement(2826..4349)
                     /locus_tag="PKN31_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99331.1"
                     /translation="MSKILQTIGLTKSFPGVLANDHVDLEIEEGEIHGLLGENGAGKS
                     VLMSTIYGLYRPDEGKILIRGKEVVIDSPHTAIKHGIGMVHQNLTLVPSFRVWENIVL
                     GKEIKKGLSLNEEETLRKLGELLKVASFEIDLRARTEELSLGSQQRIEILKVLYRGAE
                     ILIFDEPTTILTPQEVDQLMETFRSLKKEGKTIIFISHKLKEIFAVCDRVTVLRKGKK
                     VGTLPIEEATPEKLAEMMVGRKVLFSLPRGREPGEEVILKLENITTPEEKGRVTLRDI
                     NFNLHEGEILGIAGVEGNGQRELAELIVGLIKPQTGKIIFQGEDITFLSPKERIKRGI
                     AHIPDDRLRRGIIGDFTVWENLIIGFEDQPPFIKGKLTLNHSFLRQFSAQKIKEFEID
                     TPGENALVKNLSGGNQQRVVLARELSGNVALLVASQPTRGLDIAATEYLRNKLIELKN
                     QNKGVILISADLDEIRSLSDRVLVLYEGKIMGELSPQSDEYQFGLLMGGKHIDEAFI"
     gene            complement(4351..5373)
                     /locus_tag="PKN31_05085"
     CDS             complement(4351..5373)
                     /locus_tag="PKN31_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA99332.1"
                     /translation="MKKRSFLMLIIMVFLLLLPSVCWGEGKKIGLILATGGLGDKSFN
                     DISYAGAVRASEELGVELDYVEPQAVAEFEGYQRDFASSQEYEIIVCVGFDQADALDK
                     IAQEFPEQKFALVDMTVDQPNVASLLFKANEGGFLLGVQAAYMSETKKIGIVGGMDIP
                     LIRDFFIGFREGVKWADTGAEVLPTAYVGAWTDPTKGKELALSLIDQGCDVIWAAAGK
                     SGLGALEATKERGVYGMGVDACQCYLGERIIASETKRVDIAVYETIKSAFEGTFQGGI
                     LEKGVAEGWISNCRFPEEQSFWEETFNFKHQVEIPSEVMQKVEEAREKISKGEIKVPR
                     PEEFGG"
     gene            complement(5756..6451)
                     /locus_tag="PKN31_05090"
     CDS             complement(5756..6451)
                     /locus_tag="PKN31_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOA99333.1"
                     /translation="MLEELKQKVFEANLLLPRNNLVVLTWGNVSEIDRESNLVVIKPS
                     GVSYEKLRPEMMVVVNEKGEVVEGNLKPSVDTSTHLELYRQFKDIGGVAHTHSTWATI
                     WAQAGRSIPCLGGTHADHFADEIPCTRALTKKEAESNMELNTGKVIVETFKNLDYHEI
                     PAVLEAFHGPFTWGKDSLEAVQNSIVLEEVAKMSYFTLELNPHAVMPEEIIKVRYLRK
                     HGAQAYYGQSITL"
     gene            complement(7123..8529)
                     /locus_tag="PKN31_05095"
     CDS             complement(7123..8529)
                     /locus_tag="PKN31_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOA99334.1"
                     /translation="MKPKENKMGVMPIKKLLINMSLPVMISMFVQALYNVVDSIFVAQ
                     ISEEALTAVSLAFPIQNLMSSIGVGTGVGINALLSRSLGEKNFKESNRAANNGIFLGF
                     LSYLVFLIFGIFFTEIYFKAQTKDLEIFSYGISYLNIICTFSIGVFFQIIFERLLQST
                     GKSFYSMIAQGTGAIINIILDPIFIFGYFGFPKMGVAGAAIATVLGQIVAAILAIIFN
                     IKVNHEITIKIKGFRPCLKTIQKIYSVGVPSIIMMAITSVTTYGMNTILMKFSSTAIA
                     VFGVYFKLQSFVFMPIFGLNNGMVPILAYNYGAQKKERILKAIQLSIIYASCIMLLGF
                     IVIQLFPKQLLSLFNASENMLKIGVPALRIISLSYIFAGFCIICGSIYQAFGNGLLSL
                     ITSALRQLVVLLPSAYLLSLLGNVNFIWWSYPIAESTSLILSIVFLKYIYDKKIQTIE
                     DRQKSVSYSNTENSMNYI"
BASE COUNT         2813 a         1912 c         1556 g         2584 t
ORIGIN      
        1 caatcataga ttatcatctc tttttgaatt tgtcaatagg ggtgttaaga taactcatcc
       61 atgagttatt tgacgccctc ttaagataca ttgacagaag gaagtatcat cgcctctgaa
      121 tgcctctatc aggggtccat ggttttaaaa gcatttagat ttccaataaa ggatttcggg
      181 gatgacggaa aaaggcgagc acagcaggtt ttgtacgcaa ggctttgctt tttggtccta
      241 tctagcttga agttttttcc tccgccaagg ttaattgtta caacaggttt tacagaacgc
      301 tataataaat tctccctatt ggtgagaatc ttgaaggtaa gcaaatgata aaaagggact
      361 tcgggataaa agaagtaaaa gataaaagta aatgaatatc tggagagaag cactgaccag
      421 gcggggaaga agaaggatgt tccagggtat tccgaaaata gaatgcagaa aataagaggt
      481 gaggaaaatc cctacagcta tttgaggaat agctatagac caaaaaattc tcttcctgag
      541 aggttgtgaa ctacggtgaa ataggagccc gggaagcagc ccgtgcaaaa tagcagcaag
      601 ggtaaagtga ggcaaataag gccccatagg atttagaaag aaacctaaaa agtcagatag
      661 ccctcctacc agagctcctg accctggccc aaaatatatc ccggccatta tcaccggtaa
      721 ttggccaaag ccgatacgaa ttacctccac tccccccagg ggaatacgga tactggctaa
      781 gcgggttaaa acaatactta aagtggtaag aaaccccaga taaaccagag cttttgttct
      841 tttcactttt gttgtatacc tccgtatgat ctacttttta atatagggtt tataatcaga
      901 aggaggagta gcttttccta ctaaaccggt caaaaccaga atggttatga gataagggag
      961 gagcaaaata atctggggag gtaatatccc cagagtctgg attctcatct gcagagcatc
     1021 agcaaaccca aagagaagac tggccaagaa acaccctatc ggagtccatt taccgaaaat
     1081 catggctgcc agggcaataa atcctctacc gctggtcatg ccccaggcaa aaaaattaag
     1141 atgggcaaga gacagaaata ctcctcccaa tcccgctaaa gtagaaccta aagtaacaaa
     1201 aatcattttg tagagatgca cgttaattcc tgctgtgtca gctgattgag gattttcccc
     1261 tactgctcgc aatctcaagc cccagggagt ataaaacaag aaataatgag aaagaataaa
     1321 agctaaaaaa gaaatataaa ccagaatggt attattgttt aaaagaggtc caagataggg
     1381 gattttatcc agaaaagaaa aatgaagagg aggaagtcct gctactactc cggaggctcc
     1441 tcgagaaccc caaattttct gacatacgaa agcactaaag cccaatccaa aaatatttat
     1501 tcctgttcct ataacaatct gatcggcata atattttacg gttagaagag ctaaaattgc
     1561 tcccaatgcc attccagaaa gcactccagc taaaagcccg attcccggac taccggaaaa
     1621 atgagtggca aatactgccc agaaagcaga gttgagcata attccctcta aaccaatatt
     1681 aacaaccccc gctctttcac aaaacattcc ccctaaagca gcaaaggtaa gaggagtggc
     1741 catacgaatg gtagcttgta acatactaag gttaaatagc actcctatct ccatcttata
     1801 aatcctttct tctcataatg actgttttta taaagtaagg agcagcaacg aggattatga
     1861 ccagagattg aataattaaa gaaaggtcca gaggtacttt ggtgactcga gtcatggtca
     1921 tacctcccgc ccggagcata ccgaagagaa tagaagctaa aatacagcct cctggatgga
     1981 gttttcccac taaaccaacc gcaattccat cccatccgta tcccggagaa aacccatcta
     2041 caaatcgacc atgcactcct aatatttctc ctcccccacc tatgccagca ataaaaccgc
     2101 tcagaaggaa agccaaaatc tggcgatttt tgatagctat gcctttgttt tctgccgctt
     2161 cgggatttaa gcccactgcc cggacctgaa aacctaagta ggtgtgaaac aggaaaaaag
     2221 acactaaaaa ggaaaaaaat aaagcaataa taaaagaaag agagagctgg gtggggggaa
     2281 ggattttggg aagaagagca cttttggcta caggttcagt ttgggctacc caacccggag
     2341 ctttaaaagg ataattaacc aggtagctgg taagataaga agcaatatag ctcatcatca
     2401 tagtggtgat aacttcatgc actcctaaat aagctttcaa gagagccgga attaaccccc
     2461 ataatgctcc catcactcct cctgcaagaa gagaaagggc aatatgaaga ggagcaggga
     2521 gaccggagaa atatattcca gctaaagcgg aggctatagc ccccattaaa aattgccctt
     2581 ctactccaat attgaaaatt cccgtccgaa aagtaagaag aaatgctaag gcagtgaaaa
     2641 gaatgggggt agcctgggtt aaagtttgcc cccaggcatt tttactgcca aaggctcctc
     2701 gaaaaagagc agaaaaagca accagaggtg aataaccaga aaataatagt attagagcac
     2761 tgattaaaaa agccagaagg atagctataa tttccgggag aatagaaaaa gaggaaagag
     2821 attttttaga taaaagcttc atcgatgtgt ttccctccca taagaagccc gaattggtat
     2881 tcatcacttt ggggagaaag ctcacccata attttacctt cgtatagcac taaaactcta
     2941 tcgcttaaag aacgaatttc gtcaaggtcg gcagatataa gaataactcc cttgttttga
     3001 tttttgagct ctattaattt gtttcttaaa tattcggtgg cagctatgtc caagcctctc
     3061 gtaggttgag aagccaccag aagagctaca tttcctgata actcacgagc taaaaccact
     3121 ctttgctgat ttcctccgct taagtttttt accagcgcgt tttctcctgg agtatctatc
     3181 tcaaattctt tgattttctg ggcggaaaat tgccttaaaa aagaatggtt gagagttagt
     3241 tttcctttta taaagggggg ctggtcttca aaaccaatga ttaaattttc ccagacggtg
     3301 aaatcaccta ttattcctcg ccgcaggcgg tcgtcaggaa tgtgagcgat acctcttttg
     3361 attctttctt tgggagaaag gaaggtaatg tcttctcctt gaaagatgat tttccctgtc
     3421 tgaggtttta tcaatcccac aatcaactca gccagctctc tctgtccatt tccttccact
     3481 ccggcaatgc ctaaaatttc tccctcgtga agattaaagt taatatcccg gagggtgact
     3541 cttccttttt cttctggagt ggttatattt tccagcttca ggataacttc ctctcctggt
     3601 tctctccctc gggggaggga aaatagcact ttccttccca ccatcatttc tgctaatttt
     3661 tcgggagtgg cctcttcaat gggtaaagtt cccacttttt taccttttct caacaccgta
     3721 actctatcgc agactgcaaa tatctccttc aatttatgag agatgaagat tatagttttc
     3781 ccttcctttt tgagacttcg gaaggtttcc attagttgat ctacttcctg gggggtgaga
     3841 atggtggtag gttcatcgaa aataagtatt tccgcccccc ggtagagaac cttcagaatt
     3901 tctattcgct gttgggagcc taaagagagt tcttcagtcc tggctctcag gtctatctca
     3961 aaactggcca cttttaatag ctctcccagt ttgcggagag tctcttcttc atttaaggat
     4021 aggccttttt tgatttcttt tcccagaaca atgttttccc atactcgaaa agaaggaacc
     4081 agagtcagat tctggtgtac cattcctata ccatgtttaa tagcggtatg gggagaatca
     4141 attactactt cttttcctcg tatcagtatt tttccctcat cggggcggta aagcccgtag
     4201 atagtgctca taagaactga ctttcccgcc ccattttccc ccagtagtcc gtgaatttct
     4261 ccttcttcta tttccagatc aacatgatca ttagctaaaa ccccggggaa gcttttagtg
     4321 agaccaatgg tttgtaagat tttggacatt tcagccgcca aattcttctg gacggggaac
     4381 ttttatttct cctttactta ttttttcccg ggcttcctca actttttgca tcacctcaga
     4441 aggtatttct acttgatgct tgaaattgaa agtttcttcc cagaaagatt gttcttcagg
     4501 gaagcgacaa ttacttatcc acccttcagc tactcctttt tccagaattc ctccctggaa
     4561 agtaccctca aaggcagatt tgatggtttc atacacggca atatccactc ttttggtttc
     4621 actggcaata atacgctctc ccagatagca ctggcaggcg tccactccca taccatatac
     4681 gcctctttct tttgtagcct ccagcgctcc cagtccgctc tttcctgctg ccgcccatat
     4741 tacatcgcaa ccctggtcaa taaggctaag agccagctct tttcctttgg tagggtcagt
     4801 ccaggctccc acatacgcag taggtaatac ttctgctccg gtgtcagccc attttactcc
     4861 ctctctaaat cctatgaaga agtctctgat taaggggata tccatgcctc ccactatacc
     4921 aatctttttg gtttcactca tataggcagc ttgtactccc agaagaaatc ctccctcatt
     4981 agccttaaaa agaagggaag ccacgttggg ttggtctact gtcatgtcta cgagagcaaa
     5041 tttctgctct gggaactctt gagcaatctt atccagagcg tcagcttggt caaaacccac
     5101 acaaacaata atttcatact cctgtgaaga agcgaaatct cgctggtagc cctcgaactc
     5161 agccacagct tgaggttcca cataatcgag ctctacccct aattcttcac tggctcgtac
     5221 tgctccggcg taagaaatat cattgaaaga tttatcccct aatcccccgg tagccaggat
     5281 aagcccgatt ttctttccct ctccccagca aacagaagga agaagtaaaa gaaaaaccat
     5341 aatgattaac atcaaaaaac tccgcttttt cattttttct tctctccttt ctttttaaaa
     5401 aattggtatc gacccaaatt attgtaacct tgaaaagggg ggaaagcaag gagtatatct
     5461 ctcctcaaag cgtagtccca agtagccttc gccctattta cttaaacact tttctcactt
     5521 tttcccctct tcagtacctt tttcttcccg gaagatcgaa cacaaacacc cctctcgtaa
     5581 acttgaaaga aaaaacccgg aaatcagatc gagataaagg acagaaattc caaacaacga
     5641 ggccggctaa gaaatccggg aaaaacctct tcactgaaat ttatcatggt gaccttacaa
     5701 ggaatattca aagaaatacc atttagcttc tccaggtctt gaaagatatt ccctcttaca
     5761 aggtaatact ttgaccatag taagcttgtg ctccgtgttt ccgcaagtaa cgaactttta
     5821 tgatttcctc gggcataaca gcgtgggggt taagttccag tgtgaaatag ctcattttcg
     5881 ctacctcttc cagaacaata ctattttgta ctgcctccag gctatctttg ccccaggtaa
     5941 aaggaccatg aaaagcctct aaaacagcag gaatttcatg gtagtccaga tttttaaaag
     6001 tttctactat cactttccca gtattcaatt ccatattgct ttctgcttct tttttagtca
     6061 atgcccgggt acaggggatt tcgtcagcaa aatgatcagc atgggttcct cccagacagg
     6121 gaatgctcct tcccgcttga gcccagatag ttgcccaggt agagtgagta tgtgccactc
     6181 caccaatatc tttaaactgg cgatagagct ctaaatgagt agaggtgtcc actgagggct
     6241 tgaggtttcc ttctaccact tccccttttt catttaccac caccatcatt tctggtctta
     6301 atttttcata agaaacacca ctgggtttga ttaccaccag gttcgattcc cggtcaattt
     6361 cactgacatt tccccaggtt aagaccacca aattgtttct gggtaaaaga agattagctt
     6421 cgaaaacttt ttgttttaat tcttctaaca tttcttcctc ctcatcaaat tcagtatata
     6481 ctcattttaa tgaaaaaagt cctccatttc aatttttaag gagaaaaaac atttttctgt
     6541 tctgaataac ctggacaata aaaagcaaaa cacgtaaaac aataagaaaa accatgttac
     6601 ttcctggcac aaaattattc tttaccctag tgctacatac agaatgttcc cttctctatt
     6661 taaaggttga gcagggtaaa ataacttatc aatttcaaat gtaaaataca tttgttatat
     6721 attacaatac ctgcgaggag acaaaaaaag gaggttatac tatttcaact aaaaatagta
     6781 taacctctgg gaataggccc tatatcctaa aaagaagttg agtattgcct ctccggaagc
     6841 ctgaattggt gagaagtttc tattccaatt gtaaattgtt gtctttgata caccacattc
     6901 actgataaac tctctgttaa tcactgcttt taaattcatc agaataacgt ttgccagttt
     6961 tggatatcgt taaataacct cttttcgtaa cttctatttt tcttgttcaa ctaaatgtgt
     7021 ccataattat atttatttcc tttgttaaca atatcgtttg acatttgtca aaaaatcaaa
     7081 acaaggcata agaaaacttt taaacaaaca ccaaaaggtt ttttatatat aattcatact
     7141 attttctgta ttagaataag aaacactttt ttgcctatct tctatggtct gaattttttt
     7201 atcatagata tatttaagaa agacaatact caatataaga gacgtgcttt ctgcaatagg
     7261 ataggaccac catataaaat ttacatttcc aagtaaagac aataaatatg ctgacggtaa
     7321 caaaaccacc aattgtctca aagcggaagt aatcaaactc aataatccat ttccaaacgc
     7381 ctgatatatt gatccacata taatacaaaa tcctgcaaaa atataactaa gacttataat
     7441 tcgtaaagca ggaactccga tttttaacat attttctgat gcattaaaca atgataataa
     7501 ttgtttagga aataattgaa ttacaatgaa gcctaacagc ataatacagg aagcataaat
     7561 aatgcttaat tgaattgctt tgagaatgcg ttcctttttc tgggctccat aattataagc
     7621 tagaattgga accatcccat tatttaaccc aaagatcggc ataaatacaa aactttgtaa
     7681 tttaaaataa actccaaaaa cagcgattgc tgttgaagaa aacttcataa gaattgtatt
     7741 cattccataa gtagtcacag atgtaatagc catcataatg atagaaggaa ctcccactga
     7801 atagattttc tgaatggttt ttaaacaagg acgaaagcct tttattttga ttgtgatttc
     7861 atgatttacc ttgatattaa aaataattgc taaaattgct gctacgatct gccctaatac
     7921 tgttgcaatt gctgcacccg ctactcccat tttaggaaat ccgaagtatc caaagataaa
     7981 aatgggatct aaaataatat taataatagc acctgttccc tgggcaatca tactataaaa
     8041 actctttcca gtagattgta gtaatcgctc aaatataatc tgaaagaata caccaataga
     8101 aaaagtgcaa ataatgttta aataagatat cccataggaa aaaatttcta agtcttttgt
     8161 ttgtgcttta aaataaatct cggtaaagaa aattccaaat ataagaaaaa ccaaataact
     8221 aagaaatcct aaaaaaattc cattattagc agctctatta gattccttaa agtttttttc
     8281 tccaagactt ctagaaagta gagcattaat tcctactcct gtacccactc caatcgaact
     8341 cataagattt tgaataggaa aagccaaaga aacagcagtt aaagcttctt cactaatttg
     8401 agctacaaat atactatcca ccacattata aagagcttgt acaaacatag atatcatcac
     8461 aggcaaagac atatttataa gcaatttttt aataggcatt acacccattt tattttcttt
     8521 tggtttcatc tcatgctttc tcctctatga caaaacaatg tcacatacag tattatcatt
     8581 ttatgttaaa aaactaattt tatttgcacc gcagtaattc tattctgcaa ctattttaaa
     8641 aatcgaatcc ttatcactca gaatctagta caattaaaaa cagattaggg gatgatgcat
     8701 atcctctcta aactattagg ttagattata tttattttaa tagtaagcgg tgttatattt
     8761 gtttccgctt ttcttcacca acactatttg acaaagaacc aatcttctct gcacttcccg
     8821 ttccggcatt gttaactaaa atatctactc ccccaaactc ttttt
//
LOCUS       DAOPSS010000065         8796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6636_length_8796_cov_6.571445, whole genome shotgun sequence.
ACCESSION   DAOPSS010000065 DAOPSS010000000
VERSION     DAOPSS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6636_length_8796_cov_6.571445"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1712
                     /gene="gguA"
                     /locus_tag="PKN31_05100"
     CDS             183..1712
                     /gene="gguA"
                     /locus_tag="PKN31_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA99335.1"
                     /translation="MSKEILRVENITKKFPGVVALDRVSFDLRRGEVHALVGENGAGK
                     STLMKILSGVYQPDEGTIYLNGQPVVFRDPVEAQRAGISIIYQELNLMPHLTVAQNIF
                     IGREPLKRGGFIDDKKMSEDAATILSQLRVDIDPDIPVHMLPLSKRQMVEIAKALSHQ
                     AEVFIMDEPTSALTESEIEELFKVIRRLRDRGVGIIYISHRLEELKHVADRVTVLRDG
                     RYVSTDEYQSVTTDQIVRKMVGRSLQEKFPPRTSVPTDKKILEVRNISRAGVLHDISF
                     DLYQGEILGIAGLVGAGRTELARAIFGADPKDEGEIFLFGEKINISSPADAIRAGIGY
                     LPEDRKLAGLALEMLLTENITLANTEEISNSLGIISNKKEKQVAEHYVNELDIRTPSV
                     FQVVKNLSGGNQQKTVLAKWLFRKAKVLIFDEPTRGIDVGAKYAIHKLIDSLAKNGVG
                     IIMISSELPEILGMTDRILVLHEGRLVGTLQTRETNQEEILNYAAGLASNTSDKTEIT
                     L"
     gene            1727..2731
                     /locus_tag="PKN31_05105"
     CDS             1727..2731
                     /locus_tag="PKN31_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99336.1"
                     /translation="MAETVSQMEKVRNMMQRFIAVAALIALFVVFSLVTDRFFTIQNI
                     LTIALQTSTLAFMGIGVTYVIITGGIDLSIGSVVALSGVVAGLAVRAGMPVLIGFLLA
                     LLAGIACGAANGLMVTKLNLPPFIATLGMMQIARGIALYVTGARAVAGLPEEFGILGN
                     GALFRTTTTLPNGRVVTKFPGIPYPVIIMVIVAIIFDFVLKHTRLGRFTYAVGSNQEA
                     ARLSGLNVNKTKIIAYIISGGLAGLTGIVTMSRIVTAQPNAGVAYELDAIATAVIGGT
                     SLMGGAGTISGTMIGAFIIGILRNGLNMQGVSYFIQQVIIGLVIIAAVAVDQLRKREG
                     "
     gene            2810..3775
                     /locus_tag="PKN31_05110"
     CDS             2810..3775
                     /locus_tag="PKN31_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99337.1"
                     /translation="MKGFKLSLVVFCVGLLFLSTSVFAQEAVSGEIAVIVKEATSSFW
                     QNVEVGASDAEKELVAQGEQIKVSFLGPAAETKIEEQVNLVENAINRNVLAIVLAPSS
                     PDALVPAVKKANEAGIPVVIIDSLLNYEEGYVSFLATDNRAAGVLCAEELIKRAGTEG
                     KVAVMSYVPGVGSEIGRVGGFIDTIEEKTNMEIVGPFYSQSDMVTALNQTTDVLAANP
                     DLTAIFGANEPTAIGMGRAIEQAGLAGQIVAIGFDGNKDLQEMVRNGTLQGIAVQNPY
                     RMGYDGVKIALAAAKGEEVPKYINTGVVFVTKDNIDSEEAQRVLY"
     gene            3939..4241
                     /locus_tag="PKN31_05115"
     CDS             3939..4241
                     /locus_tag="PKN31_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99338.1"
                     /translation="MKKRGWSIKNLFCAGLMGCFIFVMATTGFAQEGDGYFFGLATHR
                     VVNVWEALFVKSFEWYCEDHGYKCTSMEAGNDPATQITQVRRMVDMGGRWISNWCG"
     gene            4255..5064
                     /locus_tag="PKN31_05120"
     CDS             4255..5064
                     /locus_tag="PKN31_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOA99339.1"
                     /translation="MPAVEYAKEHNVPVITANVDIYSPDVLLYIGFSGFESGKILAEE
                     IVKYLRDEVEPIGEVKGTVLEIRGTMGSATGEDRHNGFVSVLDEYPEVRVITVVGDWD
                     MAQAKTAAETVLRAYEVDAIYGGNGPMAMGAVGAIEALGKNPQDYFVATIDALPEVLE
                     AIGEGKIDVALDQPCPFYTPLAVYYLVEFLEKGEEALPKPGDIVTAEDVNIEGKIHQG
                     VDIWAHDEMWAPAPVVETEYGHPWMQTRGILVTKDNYTLESLWANFELEEW"
     gene            5219..6148
                     /locus_tag="PKN31_05125"
     CDS             5219..6148
                     /locus_tag="PKN31_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99340.1"
                     /translation="MIWVILLGVFLFFGIIKPRTFLTFQNVHFILYVSSMMGFLVFAE
                     TICLLSGNFDLSIGEIAGFSAMVTAVITVKSGLPGYLGIILVVLIGGLCGAFNGLLVG
                     KIRLNPFLVTLGTSIMFDWLTMVVRRGSVLGLPAVYLAPGGARVFGVYLAIFILLGFA
                     ILLAFVLAKTIWGSNLYSVGGNIEATRRCGINTDKVVFSAFVVAGMLAGISGLLYTGY
                     MGVATSNLADGTVFMAFAGAVIGGVSLTGGRGSILNALGGTLLLGIIEAGLTMMAIDP
                     AWQGTFTGVLVLVAILLNQSRQILRDRILMGEM"
     gene            6169..6906
                     /locus_tag="PKN31_05130"
     CDS             6169..6906
                     /locus_tag="PKN31_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA99341.1"
                     /translation="MEIPLIKMEDIHKWFDRTYALRGVNFEIYKGEVVGLIGSNGAGK
                     STLIKILSGVFPPSKGRIFYHDEEIEIKTPKDAIDLGIETVHQDYSLVGERNVAQNIF
                     LGRELVRSAGFIKVLDTQKMEEVASQKVRELGLNISSPTQEVRFLSGGERQGIAIARA
                     MLFKATLVILDEPTTALSVKGCRQVLDFISQLKKNDIACIFIDHNIGRIFPLADRFVV
                     MSHGQIIENVLREDTSVEEIEDLLVSC"
     gene            7010..>8796
                     /locus_tag="PKN31_05135"
     CDS             7010..>8796
                     /locus_tag="PKN31_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOA99342.1"
                     /translation="MLYGFNGKLLRVNLSTGEIRNEAIPDDILKKYLGGAGVALHFLL
                     KELDRGIDPLGEKNKLVFSLGPGAGVKAPGMDIHMVLSKSPLTGGVGESQTPGWWGPR
                     LKKAGWDMIIIEGRAMYPSFLVIDDDRVELRGADYLWGKDTHTVQKILEKELGKDFSI
                     AQIGPAGENLVRFASIVCDLIFINNRMGMGAVMGSKNLKAIAVRGTKDVPVADEKTLE
                     EIYHYFEDNFLTNPLNFLTTQEGIASAVPSSNQDGLFSVRNAQTSFLPGGEIYSVGSF
                     SGGGSLESVPCFSCPARCKKKVVGNNKIEGHYGAPSMESIVDFGFTLDLIDPDFIFQL
                     HEFSRKYGFDTTSSGVTLGFLAECFEKGLISKEETRGIEISFGKKEGILPLLEDMVKG
                     KGWGNEVGKGVKYLSSILGKETIPFALEVKGKEIPLHEPRVKQMLGLGYAISPSGPDT
                     FVVEHDTDFDENAPQLFLDNIQALGLLERLPAPSLEAKKVRMFYYLNQVFSFMDALGL
                     CIFAFAPCRFLPFSLISPLIQAITGWEMSLFRLMKIGETRIQLERLFNLQEGIEKKED
                     WLPERFFEPVKSGPRRGLRINPQDLKEAI"
BASE COUNT         2515 a         1416 c         2191 g         2674 t
ORIGIN      
        1 ttaaagcaac cgatttcaca aattacttca aatttaacaa tattaccaaa atattaagga
       61 ctataattgc cgacaaaaag ctgttgtcaa tgatacaaaa ttattagtaa tcgattgctt
      121 gacaaaatta agtgaacggt gttagcataa tgattaaagt tttttgttat tgaggaggga
      181 ctatgtctaa ggaaatattg cgtgttgaga atataactaa aaaatttcca ggagtagtag
      241 ccttagatcg agtttccttc gatctgagac gaggcgaggt tcatgctctc gtaggagaaa
      301 atggtgcggg aaaatcaacc ttgatgaaga tattaagtgg ggtatatcag ccagatgaag
      361 gaaccatcta tcttaatggc caaccggttg ttttccgtga tcctgtagaa gctcagcgag
      421 ccggtattag tattatttat caggaactaa atctgatgcc tcatttgaca gttgctcaga
      481 atatcttcat cggtagagag cccctcaaga ggggtggttt catcgatgac aagaagatga
      541 gtgaagacgc agccaccatc ctcagtcagc ttcgtgtaga tattgatccc gatatccccg
      601 ttcatatgct tcctctttcc aaaaggcaaa tggttgaaat agctaaagct ttgtctcatc
      661 aagcagaagt ttttattatg gatgagccta cttcagctct aactgaatcg gagattgaag
      721 aattatttaa agttatccgt cggcttcgag accgaggggt aggtattatc tatatttctc
      781 atcgacttga ggaattaaaa catgttgcag atcgagtaac tgtactgcgg gatgggagat
      841 acgtaagtac tgatgaatac caatcagtta cgactgatca gattgtacgt aagatggtgg
      901 ggaggtcttt gcaggagaaa ttcccaccga gaacctcagt tcccacggat aaaaaaatat
      961 tagaggttcg aaacatatcc agagcgggag tattacatga tatctctttt gatctttatc
     1021 agggtgaaat tttaggtata gctggacttg taggggcggg aagaactgag ctggcccggg
     1081 caatttttgg agcggatcca aaagatgagg gcgagatttt tctttttgga gaaaaaatta
     1141 atatttcttc accagctgac gctattcgtg ctggaattgg ttatcttcca gaagatagaa
     1201 aattagctgg cttggcactt gaaatgctgc ttactgaaaa tattacctta gccaatactg
     1261 aggagatatc taatagttta ggaataatct ctaataaaaa agaaaagcag gtggccgaac
     1321 attatgtaaa tgaacttgat atccgcactc ccagtgtttt tcaggtggta aaaaatctta
     1381 gcggtggtaa ccagcaaaaa acggttttag caaagtggct atttcgtaaa gctaaagtgt
     1441 tgatttttga tgaacctacc agaggaatcg atgttggagc taaatatgcc attcataagc
     1501 ttattgatag tttggctaaa aacggagtag gaattattat gatttcttct gagcttccgg
     1561 aaattttagg tatgaccgat cgaattttag ttcttcatga ggggcggtta gtcgggactc
     1621 ttcagacaag ggaaactaat caggaggaga ttttgaacta cgctgccggt ttagcttcta
     1681 atacttctga caaaacagaa ataaccttat aaaggagggt taagttatgg ctgagacggt
     1741 atcacagatg gaaaaggtaa ggaatatgat gcaaaggttc attgcggttg ctgcgcttat
     1801 agctttattt gttgtttttt cgttagttac tgatagattt tttactattc agaatattct
     1861 cacaattgcc cttcaaacca gtactctggc ctttatgggt ataggagtga cttacgttat
     1921 tatcactgga ggcatagatt taagcattgg ttcagtggtt gccctgagtg gagtggtggc
     1981 tggtttggca gtacgagctg ggatgccagt attaattggt tttttgcttg ccttgctggc
     2041 tggcatagct tgtggggcgg ccaatggtct tatggttaca aaattaaatc taccgccttt
     2101 tattgctact ttagggatga tgcaaatagc tcgaggtatt gccctatacg tgaccggagc
     2161 ccgagcagta gctggtttac cggaggagtt tggtatatta ggcaatggcg ctctttttag
     2221 gactaccacc acgttaccca atggtcgagt agttactaaa tttcctggca ttccatatcc
     2281 ggttattatt atggtcatag ttgccattat ttttgatttt gtgctcaagc atactcgttt
     2341 ggggcgtttt acctatgcag taggttctaa ccaagaagct gcccgcctct cgggattaaa
     2401 cgttaataag accaagatca ttgcctatat tatttcggga ggtttagctg gtttaacagg
     2461 aatcgtgact atgtctcgta ttgtaactgc tcaacccaac gccggggtag cgtacgaact
     2521 tgatgctata gctactgcgg ttattggtgg taccagcctt atgggagggg caggtactat
     2581 ctccggcact atgattggag cttttattat tggaatcctg cgtaacggac tgaacatgca
     2641 aggagtatcc tactttattc agcaggtgat tattggttta gttattattg ctgcagtggc
     2701 ggtagatcag ctccgaaaac gagagggtta ggtttatagg gagagtagaa aggggcagat
     2761 tttagtcctt ttgtacatta aaaaacatat aaaaggagga aggtttacaa tgaagggttt
     2821 taagttatct ttagtagtat tctgtgtggg tttgttattt ctttcaactt cggtttttgc
     2881 tcaggaagca gttagtggcg aaatcgcagt tatcgtgaaa gaggcaactt cgagcttttg
     2941 gcaaaacgtg gaagtaggcg cgtcggacgc agaaaaagaa ttagttgctc aaggagaaca
     3001 gattaaagtt tcatttctcg gtcccgctgc tgaaacaaaa atcgaagagc aagttaacct
     3061 ggtggaaaat gctataaacc ggaatgtctt ggctatcgtt ttagctcctt caagccctga
     3121 tgcgcttgtt cctgcagtaa aaaaggctaa tgaagctggt ataccagtag taattattga
     3181 ttcgcttttg aattacgaag agggttatgt ttctttcctg gctactgaca accgagctgc
     3241 tggagttctt tgtgcggagg agctaattaa acgagccggg accgaaggta aagtagcagt
     3301 catgtcttat gtgccgggag ttggtagcga aattggccgg gtaggcggtt ttatcgatac
     3361 tattgaagaa aagactaata tggaaatcgt ggggcctttc tattctcaat ctgacatggt
     3421 gactgctctt aaccagacca ccgatgtatt agcagctaat cctgacctta ctgctatctt
     3481 cggtgcaaac gaacctaccg caattggtat gggacgggca attgagcaag ctggtttagc
     3541 cggtcaaatt gtagctattg gatttgacgg taataaagat ctgcaagaaa tggtgcggaa
     3601 tggtactctt caaggtattg cagtacaaaa cccctatcgg atgggttatg atggggtgaa
     3661 aattgcttta gctgctgcta agggggaaga ggtgcctaaa tacataaaca caggagtagt
     3721 ttttgtaact aaagataata ttgattcaga agaggctcaa cgcgtattgt attaatcgta
     3781 ttaatcggca gaaacctggt ttggtcgggc gccaatccaa cccggccaag ccaggtgttt
     3841 ttttatgcat aaataaaaac cgggaggtgg taaaaaaaag tttatgataa ggttagtgtt
     3901 aattaaataa attattaata gattaatgga ggtaaatcat gaaaaaaaga ggatggtcga
     3961 ttaaaaactt gttttgcgca ggattgatgg gatgctttat ttttgtgatg gcgaccacag
     4021 gttttgccca agaaggggat ggatactttt ttggtttagc tactcatcga gtggttaacg
     4081 tttgggaagc tctgtttgta aaatccttcg agtggtactg tgaagatcac ggttataagt
     4141 gtacttctat ggaagctgga aacgatcctg ctactcaaat cactcaagtt agacgaatgg
     4201 tggatatggg gggtagatgg attagtaatt ggtgcggttg aatctaccgc cctaattcca
     4261 gcagtagagt atgctaaaga gcataatgtg ccagtgatta ctgctaatgt agatatttat
     4321 tcacccgatg ttcttcttta cattggtttt tcaggctttg agtccgggaa aatattagct
     4381 gaagaaatag ttaagtatct ccgagatgaa gtggaaccaa ttggcgaggt aaaagggact
     4441 gttttagaaa taagaggaac catgggcagc gctactggag aagacaggca taatggattt
     4501 gtaagcgtat tggatgaata tcctgaagtg agagtaatca ccgtagtggg agattgggat
     4561 atggctcaag ccaaaaccgc ggcagagact gtacttcgag catatgaggt agatgcaatt
     4621 tatggaggta atggaccgat ggccatggga gcagtaggag ccatagaagc attgggcaaa
     4681 aacccccagg actactttgt tgccaccata gatgctttac ccgaagtgtt agaagccatt
     4741 ggtgagggga aaatagatgt agctttagat caaccatgtc cattttatac tcctcttgct
     4801 gtgtactact tggttgaatt tctggagaaa ggggaggaag ctctgcccaa accgggggac
     4861 atagtcactg ctgaggatgt taacattgag ggaaaaatac accaaggagt agatatttgg
     4921 gctcacgatg agatgtgggc tccggctccg gtggttgaga cggaatatgg tcatccttgg
     4981 atgcaaacca gaggaatttt ggtcactaaa gacaattaca ccctggagtc tctttgggct
     5041 aattttgagt tagaagaatg gtgattcaga agtaagttta agaggggttt gtcttgacta
     5101 cttcaaacaa atccctctta aatactgttt tttgaaagaa gtgctttctg agaaaaagaa
     5161 gtaaggagaa catacagaaa tgaattcaaa aaagctaaaa ttgttcttcg ttgataatat
     5221 gatttgggta atattgcttg gagtgtttct tttctttgga ataataaaac cgcgtacatt
     5281 tctcacgttt cagaatgttc attttattct ctatgtttca tcgatgatgg gttttttagt
     5341 ttttgccgaa actatttgtc ttttaagtgg taattttgac ttatctatag gggaaattgc
     5401 tggcttctca gccatggtta cagcagttat cacggttaaa agtggtcttc caggatattt
     5461 gggtattatt ttagttgtcc ttataggagg gttatgtggg gcgtttaatg gattgttagt
     5521 ggggaaaatc cgtttaaacc cttttttggt tactcttgga acctctatta tgtttgattg
     5581 gcttaccatg gtagttcgga ggggttcagt tctcggttta ccagctgtat atctcgctcc
     5641 gggcggagcg cgtgtatttg gcgtgtattt agctattttt attcttttag gatttgctat
     5701 tttgctggcg ttcgttttgg ctaaaactat ttggggtagc aatctctatt cagtgggagg
     5761 aaacattgaa gctactagaa gatgtggaat taatacggat aaggtagttt tttcagcttt
     5821 cgttgtagct ggaatgctgg ctggtatttc cggactgctt tacaccgggt atatgggagt
     5881 tgccacttct aatctggctg atggcacagt atttatggca tttgctggtg cggttatagg
     5941 tggagtaagc cttactggag gaagaggctc tatcttgaat gcacttggtg ggacgctact
     6001 actaggaatt atcgaggccg gacttactat gatggcaatt gacccggcct ggcaaggaac
     6061 tttcaccgga gtattagtgt tagttgctat tttgctaaac cagagtagac aaatcttgag
     6121 agatcgaatt ttgatgggtg aaatgtagat ttcaggaggt gagaatttgt ggaaatccct
     6181 ttgattaaga tggaagacat ccacaaatgg tttgatagaa cttatgcttt gaggggagta
     6241 aactttgaaa tatataaagg agaggttgta gggttaatcg gaagcaatgg ggcgggaaaa
     6301 tcaactttga taaagatatt atctggagtc tttcctcctt cgaaaggaag gattttttac
     6361 catgacgaag aaatagaaat taaaactcct aaagatgcta ttgatttggg aatagaaacg
     6421 gtgcaccaag attactccct cgttggggag agaaacgtcg ctcaaaatat ttttctaggt
     6481 cgagaattgg ttaggtctgc tggttttatc aaagtgttag atacccaaaa aatggaagaa
     6541 gtagctagtc aaaaagtaag agaattggga ttaaacattt cctccccaac tcaagaagta
     6601 cggtttctct ctgggggtga aagacagggg atagccatag ctcgagctat gctttttaag
     6661 gctaccctgg taattttaga tgaacctact actgctttat cagtaaaagg gtgccgtcaa
     6721 gtattagatt ttatctctca gttaaagaaa aatgacatag catgcatctt tattgaccat
     6781 aacattggtc gtatatttcc tttagctgat aggtttgtag ttatgtctca tggccaaata
     6841 attgaaaatg ttttgagaga ggatacgtcg gttgaagaga tcgaggactt gttagtttct
     6901 tgctgagtca aatgttaaat ttgctttctt aatgaagata cactcgagtg gatgttccat
     6961 catttgcttg caagtatttt atgcgcatac ataaggaagg aggaaaactt tgctttacgg
     7021 atttaatggg aaattattac gagttaatct ttctactgga gaaattagaa atgaagctat
     7081 tccagatgat attctaaaga agtatctggg aggggcaggg gtagccctcc actttctttt
     7141 gaaggaattg gataggggga tagacccctt aggagagaag aataagttag ttttctcttt
     7201 aggtccgggt gcgggagtga aagctccagg gatggatata catatggttt tatctaaatc
     7261 tcccctcact ggaggagtcg gggaatcaca aactcctggt tggtgggggc cacgcttaaa
     7321 aaaagctggc tgggacatga taataataga aggaagagca atgtatcctt ctttcttggt
     7381 aatagatgat gatagggtag agctgagagg agcagactat ctgtggggca aggatactca
     7441 taccgttcaa aagattctgg aaaaagaact gggcaaagac tttagcattg ctcagattgg
     7501 gccggcagga gaaaatttag tgcgatttgc ttctatcgtt tgtgacctta tatttattaa
     7561 taaccgcatg ggtatgggag cggtaatggg gtctaagaac ctgaaagcga tagcagtgag
     7621 gggaactaaa gatgtcccgg tagcagatga aaaaacactg gaagaaatat accattattt
     7681 tgaagataac tttttgacca atcctctcaa ctttttaact actcaagaag gtattgcttc
     7741 tgctgtgcct tcctctaatc aggatggcct tttttcggtt cgtaatgctc aaaccagctt
     7801 tttacctgga ggggaaatat atagcgtggg gagtttttca ggagggggga gtttagaatc
     7861 agttccttgc ttctcttgcc ctgctcggtg taagaaaaaa gtggtaggca ataataagat
     7921 agaaggacat tatggagctc cttctatgga aagcatagtg gatttcggtt ttactttgga
     7981 tttaatagac ccggacttta tttttcagct tcatgaattt tctcgaaagt atggttttga
     8041 taccacttcc tcaggagtaa cccttggttt tttagctgaa tgcttcgaaa aagggttaat
     8101 ttctaaagag gagacgagag ggatagaaat tagttttggt aaaaaggagg ggattttacc
     8161 tcttttagaa gacatggtaa aagggaaagg ctggggaaat gaggtaggga aaggggttaa
     8221 atatttgagc agtattttgg ggaaggaaac tattcctttt gctttagaag taaaaggaaa
     8281 ggaaatacct ctccatgagc caagggttaa acaaatgctg gggctggggt atgccatatc
     8341 accctctggt cctgacactt ttgtagtaga acacgatacc gattttgacg aaaatgctcc
     8401 tcagcttttc ctggataata tccaggcttt aggactttta gaaagattgc ctgctccttc
     8461 cctggaagcc aaaaaagtgc gaatgtttta ttaccttaat caagttttca gttttatgga
     8521 tgctctgggt ctttgtatat ttgcttttgc tccctgtaga tttttacctt tttctttgat
     8581 ttctccctta atacaggcta taaccggctg ggaaatgagc ctttttcgat tgatgaaaat
     8641 aggggagacg agaatacagc tggagcgatt gtttaatctc caggaaggaa tagaaaaaaa
     8701 ggaagattgg ttgccagaga gattttttga gccggtaaag agtggtcctc gccggggttt
     8761 gcgcattaat cctcaggatt tgaaagaagc tataga
//
LOCUS       DAOPSS010000066         8694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6745_length_8694_cov_7.715592, whole genome shotgun sequence.
ACCESSION   DAOPSS010000066 DAOPSS010000000
VERSION     DAOPSS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6745_length_8694_cov_7.715592"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1049
                     /locus_tag="PKN31_05140"
     CDS             6..1049
                     /locus_tag="PKN31_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA99343.1"
                     /translation="MAEKVKWGVIGAGGIARRRTIKETIMYAQMAEIKALMDIDEKVV
                     KEVGREWGIDRWFTDVEKLLQEDIEAVYIASPNYVHYQQAKASLEAGKHVLCEKPLSL
                     TLEEGKELALIAEEKGLKLGVAFMMRFNVYHKEIKRLIEEGALGQLVAGRAQLTCWYP
                     PLPSAWRQDKEKGGGGALIDMGCHCIDLLEWFFGETIEVAAFLDTITHSYLVEDTSTA
                     ILKFRNGAQGIVDNYFNVPDKAAKNILEVYGTKGAVIAHQTIGQDPGGKMWLYQEKGE
                     KSYNAEQEREENAYKEIKLEPLSLYAQEIDAFSQWIRGGEKPVISYEVGLRNSKVIEA
                     IYRAQEEKRVITV"
     gene            1404..1649
                     /locus_tag="PKN31_05145"
     CDS             1404..1649
                     /locus_tag="PKN31_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99344.1"
                     /translation="MIETFRNDGRYSGVTTKQRHPHPNLLPRRERRRIKKIEGEIRKR
                     LIKRNFYGSSALIVLVNDNTTLKKRVCENIPESELNL"
     gene            complement(1639..1797)
                     /locus_tag="PKN31_05150"
     CDS             complement(1639..1797)
                     /locus_tag="PKN31_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99345.1"
                     /translation="MVYNWINLIIGIVTIICPIAAGVKGASLWLHIVIGIIIVLCAYM
                     ANRGVAKG"
     gene            complement(2092..2694)
                     /locus_tag="PKN31_05155"
     CDS             complement(2092..2694)
                     /locus_tag="PKN31_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOA99346.1"
                     /translation="MFFLKKEEEVELVINRSRFVGKSLEVRSREEVKERLHEVMKLFP
                     GATHYCYAFRIGENTVEEFSSDAGEPPGSAGRPILGAIRRGDFTDTMVVVVRFFGGKK
                     LGIRGLIDAYGKTAQLVLEKSGKKAYREIEFLVKIDPVYFSLVVNQLVEVVQDKQLLS
                     IYPDRGEVHLRISQSEEEKIKEILEEGERTGKILEGRRVR"
     gene            complement(2804..3733)
                     /locus_tag="PKN31_05160"
     CDS             complement(2804..3733)
                     /locus_tag="PKN31_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOA99347.1"
                     /translation="MPPLKELAKRPERLTSGHRLCAGCGAPIAIRLILAAADKPVVAA
                     NATGCLEVSTTIFPYTAWNIPWIHSAFENAAATITGVETMYRAKKKRGEIDEDIYFVA
                     FGGDGGTYDIGLQSLSGAWERGHRFVYVCYNNEAYMNTGIQRSSATPLGAWTTTSPVG
                     KVLPGKTQWRKNLTEIAVAHDIPYVAQSVPSNWRDLTLKAEKAFHTEGPAFLNVLASC
                     NRGWRHLPADTLKVNQLAVDCCIWPLYEVENGEYRLTYKPREKKPVEEWLKVQGRFQH
                     LFSPAYRYLIDEFQAYVDREWERLLEKCGETKK"
     gene            complement(3748..4929)
                     /locus_tag="PKN31_05165"
     CDS             complement(3748..4929)
                     /locus_tag="PKN31_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOA99348.1"
                     /translation="MAKVVGFNGNAAVAYAMKQINPDVVAAYPITPQTDCVERFAEYV
                     SEGLVDSEFITVESEHSAMSACIGAAAAGGRVMTATAANGLALMWEMLYIAASMRLPI
                     VMMVTNRALSGNINIHCDHADTMGARDAGWIHLFSENAQEAYDNTIQAIKIAEDLGVR
                     LPIMVTQDGFIISHAVERVEILEDEEVKNFIGAYKGRYPLLDLNNPVTYGPIDLFDYY
                     FEHKRSQIEAIDNCLPVIERVGKEFGELSGRNYELLEGYRLEDAEYAVVALGSTCGTA
                     REAIDSLREKGKKVGLLKMRCFRPFPRESVIEALSPLKGIAVLDRSVSFGGFGGPLFT
                     EVRSALYDAPSRPSIINFFYGLGGRDIYPEDIEKALERTEEVVKAGKIEQHIDYLGLR
                     E"
     gene            complement(4943..5233)
                     /locus_tag="PKN31_05170"
     CDS             complement(4943..5233)
                     /locus_tag="PKN31_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOA99349.1"
                     /translation="MSELKGWKELVPGGLIVEAGNAKEYKTGDWRTEKPELNPEKCIH
                     CFFCWAYCPDNAVIVDAEAGKMLAFNYDYCKGCGICAAACPAKGAITMVPER"
     gene            complement(5226..5801)
                     /locus_tag="PKN31_05175"
     CDS             complement(5226..5801)
                     /locus_tag="PKN31_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOA99350.1"
                     /translation="MKKPLEIRWHGRGGQGVVTASRILADATLREGKYFQSFPEYGPE
                     RMGAPVKAFNRISSQPLRMHCGVTNPEIVVVIDPTLLGLAEILEGLDEKGTIVVNFEG
                     SPQELREMLGFKGGRVVTVDASQISRDILGRVVVNTSMLGALGKVLPEVSMENLLEAV
                     RSQFGEKLRPEVIDKNLLCLTKACEEAQIDE"
     gene            complement(5807..6430)
                     /gene="recR"
                     /locus_tag="PKN31_05180"
     CDS             complement(5807..6430)
                     /gene="recR"
                     /locus_tag="PKN31_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOA99351.1"
                     /translation="MAESYPEALSRLISAFSRLPGIGPKTAQRLAFYLVKSPSLEVEE
                     LLSSLQEVKEKIHFCSVCGFYTEEDRCKICENPQRDRSVVCVVERPQDVLAIEKTGFR
                     GLYHVLQGIISPLSGIGPEKIKIKELLERVSQGEIKEVILAISPSVDGEATALYLAQK
                     LSSFPVLVTLIARGLPSGGDLEFADEVTLQEALEGRRRISLKPRKEE"
     gene            complement(6443..6754)
                     /locus_tag="PKN31_05185"
     CDS             complement(6443..6754)
                     /locus_tag="PKN31_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOA99352.1"
                     /translation="MQNWKNLMKEAQKMQAKIAKMQEELKEKTVEASSGGGMVKVVCN
                     GQQEIIDIEIEPELLEEKDVEMLQDLVLAAVNEALRRSREIAEEEMGKITGGLNLPGL
                     M"
     gene            complement(6705..8324)
                     /gene="dnaX"
                     /locus_tag="PKN31_05190"
     CDS             complement(6705..8324)
                     /gene="dnaX"
                     /locus_tag="PKN31_05190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOA99353.1"
                     /translation="MAYLSLYRKWRPSRFEEVVGQEVSVRILTHSLDKKRLHHAYLFC
                     GPRGVGKTTTARLLAMAVNCEKGITPQPCGECESCRTIQEGNSLDVVEIDAASHRGID
                     EIRELRERVKYVPLQSRFKVYIIDEVHMLTTEAFNALLKTLEEPPEHVIFVLATTEPR
                     RLPDTIISRCVKIIFNPLPEEAVVKKLEEIAREEGAAIEEEVFYLIARKAGGSMRDAE
                     GMLEQILAWGEEEPVDLATASLILREIDSEDFTRLLELIIKGDKGGALLLLNQWIQRG
                     LSPEDISHSLESYLRELLLFRLTRKQDKKREKIRKEWQSLTKQVSEEALQKALERVSY
                     LEFELRRSSQPRVLLELCLLDIIKCLAQPGLPEEKLPEENPVKEEVSQKPEENNGNFW
                     PEILQEVKKAKISLYAFLQAADFREVGSSHAVLSFSSNCQFHKESVEREENLSILKEV
                     LAKRRGGNWKIECVINEKAPEVEVEREREDRKEKREEQLSHNHKSLMGEVIDLFSGVL
                     VDYSLSEEIAKGGWEDAELEKSDEGSPEDAS"
     gene            complement(8362..8627)
                     /gene="ffs"
                     /locus_tag="PKN31_05195"
     ncRNA           complement(8362..8627)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKN31_05195"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT         2452 a         2129 c         1666 g         2447 t
ORIGIN      
        1 aaaccatggc tgagaaagtc aagtggggag tgataggagc ggggggaatt gcccgaagaa
       61 gaaccataaa agaaacaata atgtatgctc aaatggcgga aataaaagcg ttaatggata
      121 tagatgagaa agtagtgaaa gaagtaggca gggagtgggg aatagaccga tggttcaccg
      181 atgtagaaaa acttctacaa gaagatatag aagccgtata tatcgcttct cctaactatg
      241 ttcattacca gcaagcaaag gcatcccttg aagcaggaaa acacgttctt tgcgaaaagc
      301 ctctatcttt aaccctggaa gaaggaaaag agctagcgct tatagcagaa gaaaaagggc
      361 taaaattagg cgtagcattt atgatgaggt ttaatgtata tcataaagaa attaaaaggt
      421 tgattgaaga aggagctctg ggacaattgg tggcaggtag agcccagtta acctgctggt
      481 atccccctct tccctctgcc tggcggcaag acaaggaaaa agggggagga ggagcactta
      541 tagacatggg ttgtcactgt attgacctcc tggaatggtt ttttggagaa acaattgaag
      601 ttgccgcttt tttagatacc attactcatt cttacctcgt agaagatacc tctacagcaa
      661 tacttaagtt tagaaacgga gcgcaaggta tagtggacaa ctattttaac gttcctgata
      721 aagcagctaa aaatatactg gaagtctatg gaactaaagg ggcagtcatt gctcaccaaa
      781 ctatcggaca agacccggga ggcaaaatgt ggctttatca ggaaaaggga gaaaaaagct
      841 ataacgcaga gcaagaaaga gaagaaaatg cttataaaga aataaaactc gaacccttat
      901 ctctttatgc tcaagaaatt gatgctttca gtcaatggat aaggggagga gaaaaaccag
      961 taatcagcta cgaagtagga ttaagaaatt caaaggtgat agaagctatt tatcgagcac
     1021 aggaagaaaa aagagtaata acagtctgag tcctgtcctg aggaagaggg ctgggggaag
     1081 atcttacaag gagaatcgcc tcaccttctt cctctcttct tatactggta gaagattttc
     1141 tcatcagtag tatttactgt ttttccattt tcgcattttt ccgagaatta taaaagggta
     1201 tcccctttaa taatatttcc tgtcatgaaa tcattattgg gaaacttaaa agctctaaat
     1261 acctttagac ttcttataga ggcattcggg agcgacttca cttttcccgt cattcccaaa
     1321 agttttagtc gggaatttag atgcttttgc ccttaaataa tatagattcc tgatagaaac
     1381 attcaggaat gagggacttg gggatgatag aaacattcag gaatgacggg aggtactcag
     1441 gggtaacgac aaagcaaaga caccctcacc ctaaccttct ccctcgaagg gagaggagaa
     1501 gaataaaaaa aatcgaggga gagataagaa agagattgat aaagaggaat ttttatggtt
     1561 cttcagcttt aattgtcctg gttaatgata atacaacttt aaaaaagagg gtttgtgaga
     1621 atatccctga atcagagctt aacctttagc tactccccgg ttggccatat aagcacacag
     1681 aactataatt atcccgatta ctatatggag ccatagcgaa gcccctttta cccccgcagc
     1741 aatgggacaa attatggtta caatcccgat tatcaggttt atccagttat acaccatttc
     1801 tcccttctcc tttttactaa ttcttaactc tctcgcaaac cctctttgag agaattatat
     1861 ctcaacttaa aggacaaaaa tagctatcat aaccggtgga gattcgagtt tcgcggtaga
     1921 accacgcctt ttctttttgc ccaagaagag gtacaagctg tgattaatta aaataaggga
     1981 aaactggaaa atggggaatc ctgacttgaa cataattatg gagaaagaat aaagacgggg
     2041 ttattttctt ttcctaccga tggatcagtt ttttgcttac ccgggcaaat tctaccttac
     2101 tcttcttcct tctaaaattt tccctgtcct ttctccttcc tcaagaattt cttttatttt
     2161 ctcttcttcg ctttgagaaa tgcgaaggtg cacttctcct ctatcgggat aaattgagag
     2221 aagctgctta tcctgaacaa cctctaccag ttgattcacc accagactaa aataaacagg
     2281 gtcaatctta accaaaaatt ctatttccct gtacgctttt ttgcccgatt tctccaaaac
     2341 cagctgagca gttttcccat aagcatcaat caatcctctg atccccaatt ttttaccccc
     2401 gaagaaacgc accaccacca ccatggtatc ggtgaaatct ccccgccgga tagcacccag
     2461 tatgggtctt cctgcacttc ccggtggctc ccctgcgtcg gaagaaaatt cctctaccgt
     2521 attttctcct atgcggaaag catagcaata gtgagtggcg ccgggaaaaa gcttcatcac
     2581 ctcgtgtaac ctctctttca cctcttcccg agagcgcact tccaggcttt tccccacaaa
     2641 tcgagagcga tttatgacta actctacctc ttcttctttt ttaagaaaaa acattttttt
     2701 atcactattt ggagcatcaa tttaaaacat tttcaagcag cttaaaagaa gattaacagg
     2761 agccagaaag ctcgaaagct ctggctcctc tcagggtaaa aaactacttt ttagtttctc
     2821 cgcacttctc gagcagtctt tcccattccc gatctacata ggcctggaac tcatcgataa
     2881 gataacggta agcaggagaa aagagatgtt ggaaccgacc ctgcaccttc agccattctt
     2941 ctaccggttt tttctccctg ggcttataag taagtcgata ttcaccattt tctacttcat
     3001 aaagaggcca gatgcagcaa tccactgcta actgattcac tttcaatgta tcagcaggga
     3061 ggtggcgcca tccccgattg caagaagcca gcacattaag aaaagcgggg ccttccgtat
     3121 gaaaagcttt ttctgctttg agggttaaat ctcgccagtt agagggaacg gattgagcca
     3181 catagggaat atcatgagct acagcaattt cagttaaatt ttttctccac tgggttttcc
     3241 cgggaaggac tttccccacc gggcttgtag tagtccatgc tcctaaggga gtagcactgg
     3301 agcgctgaat accggtattc atataggctt cgttgttgta acacacatac acaaatcgat
     3361 gtcctctttc ccaggctcca gaaagagatt gtagaccaat atcataagtt ccaccatcac
     3421 caccaaaagc cacaaaatat atgtcttcgt cgatctctcc tctttttttc ttcgctcgat
     3481 acatggtctc taccccggtg atggtagcag ctgcgttttc aaaagcactg tgaatccagg
     3541 gaatgttcca ggcggtgtag gggaaaatag tggtagatac ctccagacat cccgtagcat
     3601 tggcagcaac tactggtttg tcagcagcag ccagaatcag tcggatggcg ataggagctc
     3661 cacatcccgc acataacctg tgccccgagg tgagtctctc cggtctttta gccaattctt
     3721 ttagaggtgg cattttctaa aaacctccta ttctcgtaaa cctaaataat cgatatgttg
     3781 ttctatcttt cctgctttta ccacttcctc tgttctctct aaagcttttt ctatatcttc
     3841 aggataaatg tcccttcctc ccagaccata gaagaaattt atgattgaag ggcgagaagg
     3901 ggcatcgtaa agagcagagc gaacttcagt aaacagtggt ccaccaaatc ctccaaagga
     3961 aacagaacgg tctaaaactg ctatcccctt caaaggagaa agagcttcaa ttacgctttc
     4021 tcggggaaag gggcggaaac agcgcatttt gagaagtccc acttttttcc ctttctcccg
     4081 cagagaatca atggcttccc gagccgttcc acaagtggaa cccagagcca ctactgcata
     4141 ttcagcatct tctaaccgat aaccttccag gagttcataa tttctcccgg aaagctctcc
     4201 aaactcctta cccactcgtt caattaccgg taaacagtta tcaatagctt cgatttgaga
     4261 ccttttgtgc tcaaaataat agtcaaaaag gtcaataggc ccgtaggtaa ccggattatt
     4321 taaatcgaga agaggatagc gccctttgta ggcaccaatg aagttcttca cttcctcatc
     4381 ttctaaaatt tctacccgct ctacagcgtg actgatgata aacccatctt gagtaaccat
     4441 aatcgggagt cttaccccta aatcttcagc aattttaatt gcctggatgg tgttgtcata
     4501 ggcttcttga gcgttttctg aaaaaagatg aatccatcct gcatctcttg ctcccatagt
     4561 atcagcgtgg tcacagtgaa tattaatatt gccagacaaa gctcggttgg ttaccatcat
     4621 cacaatggga agacgcatgc tggcagcaat atagagcatc tcccacatga gagctaaacc
     4681 gttagcagca gtggcggtca tcactcttcc ccctgcagca gctgctccta tgcaggcgct
     4741 catagcactg tgttcgcttt ctacagttat aaattcgcta tctaccaacc cttcactaac
     4801 atactcggca aacctctcta cacaatcggt ctggggggtg atagggtaag ctgccaccac
     4861 atcagggtta atctgtttca tagcataagc tactgcggca ttgccgttaa aacccacaac
     4921 tttagccatt tctctcacct ccctatcgct ccggaaccat agtaatggct cctttagcag
     4981 gacaagcagc ggcgcagatt ccacagcctt tacaataatc gtagttaaaa gccagcatct
     5041 ttcctgcttc ggcgtccact atcactgcat tatccggaca ataagcccag caaaagaaac
     5101 agtgaataca tttttccggg ttaagctccg gtttttcagt tcgccaatct ccggttttat
     5161 actctttggc attacccgcc tctacaatta atccgccggg caccaattcc ttccatcctt
     5221 tgagctcact catctatctg tgcctcctca caagctttag taagacaaag caagttttta
     5281 tctatgactt ccggtctcag tttttctcca aactgggaac gaacagcttc taacaggttt
     5341 tccatactaa cttctgggag aactttaccg agagcaccca gcatggaagt attaactacc
     5401 actcttccta aaatatctcg agaaatctgg gaagcatcta ccgtaaccac tcttcccccc
     5461 ttaaaaccta acatctctct cagttcttga ggagaacctt caaaattaac cactatagtt
     5521 cctttttcat ctaacccttc taaaatttcc gctaaaccca aaagagtagg gtctatcact
     5581 accactattt ccgggttagt aactccgcag tgcatgcgga gaggttgaga actgatgcga
     5641 ttgaaagctt ttaccggggc tcccattctc tctggcccat actcggggaa agactgaaaa
     5701 tatttcccct ctcgcagagt tgcatcagct aaaattcgag aggcagtaac tactccctgc
     5761 cctcctcgac cgtgccacct gatttctaag ggctttttca agattactat tcctcctttc
     5821 ttggtttcag gctaattctt cttcttcctt ctaaagcttc ttgcaaagtt acttcatcag
     5881 caaactccag atctccacca gaaggcaatc ctcgagcaat aagagttaca agaacgggaa
     5941 acgacgataa tttttgtgcc aaataaagag cagtcgcttc cccatctact gaaggactga
     6001 tggccaagat cacctccttt atttctccct gactcactct ttccaacaat tccttaatct
     6061 tgattttttc tggaccaatt ccagaaagag gagaaataat accctgcaga acatgatata
     6121 acccccggaa tcctgttttc tctatagcta aaacatcctg gggtctttct acaacgcaga
     6181 ctacgctccg gtctctctga ggattctcac aaattttaca tctatcttct tcagtataaa
     6241 acccacagac agagcaaaaa tgaattttct cttttacctc ctgcagggaa gacaacagtt
     6301 cttctacctc taaagaaggg gatttaacca gataaaaggc taaacgctga gcggttttgg
     6361 gcccaatacc gggtaaacga gaaaaagcag agataagcct tgataaggct tcaggataag
     6421 attctgccaa cgatgttcct ctctacatca aaccaggaag gtttaacccc ccggtgattt
     6481 tccccatctc ttcttcagct atttctcgtg aacgacgcaa agcctcgttc accgccgcca
     6541 gaaccaggtc ttgcaacatt tccacgtcct tttcttccaa taattcgggc tcgatttcaa
     6601 tatctattat ctcctgctgt ccattacaaa ctacctttac catcccacca ccagatgaag
     6661 cttccaccgt tttttccttc agctcctcct gcatcttggc tatcttagct tgcatcttct
     6721 gggcttcctt catcagattt ttccagttct gcatcttccc acccaccttt cgcaatttcc
     6781 tcacttaagg aataatcaac taacaccccg gagaagaggt caataacctc acccatcaat
     6841 gacttatgat tatgagaaag ttgctcctct ctcttttctt tcctgtcttc tctttccctc
     6901 tctacttcta cttcaggagc tttttcattt ataacacatt ctattttcca attccctcct
     6961 cgtcttttag ccaggacttc tttaagaatg ctcaagttct cctctctttc aacgctctct
     7021 ttgtgaaatt gacaattaga gctaaacgat aaaacagcat gagaagaacc tacttctcta
     7081 aagtcagccg cttgcaaaaa tgcgtaaagg gaaattttag ctttttttac ctcttgcaaa
     7141 atttccggcc agaaattacc gttattctct tctggtttct gactaacttc ttcttttacc
     7201 ggattttcct caggtaattt ttcctcgggc aatccaggtt gcgccaggca cttaattata
     7261 tccaataaac aaagttctaa caacacccgg ggttgtgaag aacgacgaag ctcaaattca
     7321 agataactta ctctctctaa agctttttgt aaagcttctt cgctcacttg tttagttaga
     7381 gattgccact cctttcttat cttttctctt tttttatcct gctttcgagt aagacgaaat
     7441 agtagcaact cccgaagata gctttccagg ctgtggctga tgtcttccgg gcttaagcct
     7501 ctttgaatcc attgattaag aaggagaagc gctcctcctt tatcaccctt gattatgagc
     7561 tcaagaagcc gagtaaagtc ctcgctatct atttctctta aaatcaagga agcagtagct
     7621 aaatctacag gctcttcttc tccccaggct aaaatttgtt ccagcatccc ttcggcatca
     7681 cgcatagaac ctcctgcttt acgagctatg agatagaaga cttcctcttc aattgcagct
     7741 ccttcttctc tggctatctc ttctaacttt ttcaccacag cttcttccgg tagggggtta
     7801 aaaatgattt ttacacagcg agaaatgatg gtatcgggaa ggcggcgagg ctcagtggta
     7861 gccagcacaa atataacgtg ttctggtggc tcttccaggg ttttgagcaa agcattaaaa
     7921 gcttcagtcg tgagcatgtg cacttcatcg atgatgtaaa ctttgaagcg agactgcaag
     7981 ggaacatatt taactctttc ccggagctct cgtatctcat ctataccccg gtgagaagca
     8041 gcatcaattt ctaccacatc cagagaattt ccctcctgga tagtccgaca actttcgcat
     8101 tccccacaag gctgaggagt tattcctttc tcgcagttaa ctgccatagc cagaaggcga
     8161 gcagtagtgg tttttcctac ccctcgcggt ccacaaaaaa gataggcatg atgaagtcgt
     8221 tttttatcta aggaatgggt taaaatacgt accgatacct cttgtcctac tacttcttca
     8281 aaacgagatg gtctccattt cctatagagt gacaggtaag ccaatgttct accttcttag
     8341 ttttcaataa actgcctcta atcgtgcacc gacattcgat aatttcttcc cagccgtagc
     8401 agaggcagca aactccggtt aggcaccctt gcggcacccg aagagcttca cgtaccgctg
     8461 ctccctcccg ggcctgacgg ggttagtaaa gattcgtcgc gcaagaccca accttcaccg
     8521 ttccgttcct ccacccggaa ctgaaaagaa aaaacctcct gtcggaattc agccctgctg
     8581 tagcggattg caggtacagg gcaccgctat ctccccgctt agcacgatta gaggcaaatc
     8641 ttggcggaga gggtgggatt tgaacccacg agacaggttc aacgcctgtc tacc
//
LOCUS       DAOPSS010000067         8548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_6890_length_8548_cov_8.533734, whole genome shotgun sequence.
ACCESSION   DAOPSS010000067 DAOPSS010000000
VERSION     DAOPSS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6890_length_8548_cov_8.533734"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKN31_05200"
     CDS             <1..578
                     /locus_tag="PKN31_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOA99354.1"
                     /translation="ALGIKIPYFVEVDYEGFEKVIDALGGVEIEVEKPLKYVDRAQDL
                     YIDIPAGKQLLDGEKALQYVRFRYDPLGDIGRIKRQQEFLQALLSKVDNDPLLLARLP
                     QLIESTRGALTTNLTGDYLKQLIMWFQGLNERIIKMETMPGESIYVNGVSYWEPDLET
                     SREIVQKFFFARREDIENSREDSIGQESGGE"
     gene            553..906
                     /gene="rsfS"
                     /locus_tag="PKN31_05205"
     CDS             553..906
                     /gene="rsfS"
                     /locus_tag="PKN31_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOA99355.1"
                     /translation="MAKRAAESKKAFDLVILDLRGLFPFSDYWLICSGSSAIQTKAVA
                     EEVLKEMEECQLRPSHLEGMEEGEWILIDYGDLMIHIFREGERLFYQLERLWHRAPLL
                     YSEKEGLNLLEEIKD"
     gene            1027..2220
                     /gene="metK"
                     /locus_tag="PKN31_05210"
     CDS             1027..2220
                     /gene="metK"
                     /locus_tag="PKN31_05210"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOA99356.1"
                     /translation="MEKRYQITSESVTEGHPDKISDQVSDAILDAILAQDPQGRVAVE
                     TLVATGLILIAGQVSTSSYVDIPHVARSTVRDIGYTRAKYGFDFETCAVLTAIDEQSP
                     DIALGVNRSLEVKMGEEVEDEELSLGAGDQGMMYGYACNETPELMPFPIVLAHRLAFR
                     LAEVRKKGVLSYLRPDGKTQVTVDYVNDKPVRIDNIVVSAQHHPDISLDTLREDILRE
                     VINPVVPSSYRDDKTQVLVNPTGRFVIGGPQGDTGLTGRKIIVDTYGGISKHGGGCFS
                     GKDPTKVDRSGSYAARYVAKNVVAAGLADRCEFQLAYAIGVARPVSMSIDTKGTGKIP
                     DDEILRLVKEVFDLRPGAIIKNLKLRRPIYKKTAAYGHFGRNDPDFSWEKTDKADVLK
                     EKAGL"
     gene            2235..3491
                     /gene="ahcY"
                     /locus_tag="PKN31_05215"
     CDS             2235..3491
                     /gene="ahcY"
                     /locus_tag="PKN31_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOA99357.1"
                     /translation="MEFEVADLDLASEGKKKIEWTEREMPVVTNIASSWKDVLPLQNL
                     RIAACLHVTAETAVLVRALQAGGAEVRLCASNPLSTQDEVAASLVKDYGISVFAIRGE
                     DHDTYYQHIQKVLEFKPHITMDDGADLVSFVHVHKRDLLSEIKGGTEETTTGVIRLRS
                     LEKNGELGYPVIAVNDALTKHLFDNRYGTGQSTIEAILRATHILLAGKKVVVAGYGWC
                     GRGIAARAKGMGARVAVVEVDPVRALEALMDGNDVLSMKKAASWGEVFITATGNTSVI
                     RGEHFLSLPDGAILANAGHFNVEIDVKELEEISVNKREVRPLVEEYTLGNGKKVYLLG
                     EGRLVNLTCAEGHPPTVMDMSFANQALSVKYLKENASHLEKKVYRVPEEIDREIASLK
                     LQSLGLEIEKLTSRQREYLSSWQEGT"
     gene            3550..3933
                     /gene="rpsF"
                     /locus_tag="PKN31_05220"
     CDS             3550..3933
                     /gene="rpsF"
                     /locus_tag="PKN31_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOA99358.1"
                     /translation="MNNYELGVILRPTLSEEEVNSAVEKLKNFIEERGEVVKVDLWGR
                     RKLAYPIQKYQEGYYVFFNFKFPPSEVAELKRWMGLNEEVIRHLLVKEDEKEETSVSS
                     SSSPEEEISEEAVSEDAAGDVSSQS"
     gene            3914..4288
                     /locus_tag="PKN31_05225"
     CDS             3914..4288
                     /locus_tag="PKN31_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOA99359.1"
                     /translation="MSHLSLNRVFLIGFLPHSPVLRYVPSGIPVVNFSLAVYRTFSSE
                     EEKEWIDYLNIIAWRDLALFCGENLKRGDWVFVEGRIQVRAYESSEGKKRKAYEIVAS
                     EVKLLSYPKNRKREEVTENGSR"
     gene            4275..4526
                     /gene="rpsR"
                     /locus_tag="PKN31_05230"
     CDS             4275..4526
                     /gene="rpsR"
                     /locus_tag="PKN31_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOA99360.1"
                     /translation="MAVDRDEYSGRKKFFRRKRRPCMFCLGKAKIDYKNADFLQRFLS
                     ERGKIVPRRVTGTCAKHQRKVARAIKRARELALLPYAVD"
     gene            4532..5440
                     /locus_tag="PKN31_05235"
     CDS             4532..5440
                     /locus_tag="PKN31_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOA99361.1"
                     /translation="MRQARSTVEGAFLAALTVIFYLSSIYIPVLGFLLSFLCPLPIMF
                     LTIRWDMKAGLLAALVASFIVFIFAGAISALTCFVGFTFLGLIMGLTIKRGYNFIEVM
                     GINTLVSISSKLLLIGLVFLIMGQNPLSETLSFLEEGLKKGLSFFPQEGGIDVEMIAS
                     FIHMVLPAAVFIASLFDTVLNFLLGSWIGKRLDIKFPPFPPFENLQLPRSIFWAFALG
                     WLFVLFGGATFWGKIGLNLRMVTQVLFLVQGASVVYYFLGKYIRSRFWLTIIVVFVAL
                     QPLLSTILSWVGVFDFCFDLRKIRGK"
     gene            5452..5925
                     /gene="rplI"
                     /locus_tag="PKN31_05240"
     CDS             5452..5925
                     /gene="rplI"
                     /locus_tag="PKN31_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOA99362.1"
                     /translation="MKVILLQEVENLGKEGDMVEVKNGYARNYLLPRKLAIEASKGNI
                     TQIEALKKKRAIREAKELEEAKILQERIDGLVLEFEKKAGKTGKLYGSVTSREIAEKI
                     SQVVGINLDRKYLELGEALKEIGEYPVKVNLGKGIYATVQVKVQGEEVEEEAEES"
     gene            5922..7241
                     /gene="dnaB"
                     /locus_tag="PKN31_05245"
     CDS             5922..7241
                     /gene="dnaB"
                     /locus_tag="PKN31_05245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOA99363.1"
                     /translation="MSIERVPPHDLEAEQATLGSMLLDKEALLRVLDILQPEDFYRDN
                     HRIIFESITELFEEDQACDIVTLVAKLRQKNQLEAVGGIDYLTQLVNIVPTAANAEYY
                     AHIVEEKSIFRSVINLCTRIIRESYDADLDAKELLDRAQNWILQLSQKKIRYDFVPLK
                     VIINEAFDRIEELYHRDEHVTGVPSGFVDLDLITAGFQPSDFVVVAARPGMGKTSLCL
                     NIAQYVGTKKDISVAIFSLEMSKDQVAQRMLCSEARIDAAKVRKGFIAEREWPELARA
                     AGRLAEANIFVDDTPSISVLELRAKARRLKAEKGLGLVIVDYLQLMRGYGRPENRQQE
                     ISEISRSLKAMARELNVPVIAVSQLSRAVEQRTPKRPQLSDLRESGAIEQDADLVLFI
                     YREDYYNPNTPKKGIAEVIVAKQRNGPIGTVELMFQKDYARFENLAR"
     gene            7390..8250
                     /locus_tag="PKN31_05250"
     CDS             7390..8250
                     /locus_tag="PKN31_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOA99364.1"
                     /translation="MDKLTIYQRPLLSNPSLLVGFTGWMDGGDVSSGSVAYLQKKFAA
                     EMIAEIDPQEFYLFNFPGSMEEVAQFRPYTLIKEGIITEFHYPQNQFFCASKENLLLF
                     LGKEPNMKWNEYVENIFFLGKEFGVKRIYFIGSVSGLIPHTREPRVTCSVSDERLKKE
                     LEGYNLYFNDYEGPASITTLLTLMAPQKGVEMINLVVEIPMYIQATNPKGISSALGIL
                     SSLLNLPLVGEDLVNMSEEFDRNVEQLVKKYPDLVERIKKLEENYDREILEDDQNFRE
                     WLRRHGIDKI"
BASE COUNT         2568 a         1403 c         2075 g         2502 t
ORIGIN      
        1 gcgctctggg gataaaaatt ccctattttg tggaggtaga ttatgaaggg tttgaaaaag
       61 ttatcgatgc tctgggggga gtagagatag aagtggaaaa acctcttaaa tatgtagaca
      121 gagctcaaga tctttatata gacattcccg cagggaaaca attgctcgat ggggaaaaag
      181 cgttacagta cgtgcgcttc aggtatgacc ctctgggaga cattggaaga atcaaaaggc
      241 aacaagaatt tctacaggct cttctctcta aggtagacaa cgatcctctt ttgctggctc
      301 ggcttcctca attaatagaa agtacccggg gagccctgac tactaatttg actggtgatt
      361 atctcaaaca gttgattatg tggtttcaag gattgaatga gcgaataata aaaatggaaa
      421 cgatgcctgg ggagtcaata tatgtaaatg gagtaagcta ttgggaacca gatctcgaga
      481 cttctcgaga gatagttcag aaatttttct ttgctcggag ggaggatatt gagaattcaa
      541 gagaagatag tattggccaa gagagcggcg gagagtaaaa aagccttcga tctcgttatt
      601 cttgatttac ggggattatt ccctttttcc gattactggc tcatttgcag cggttcttct
      661 gctattcaga ctaaagcagt ggcggaggaa gtcctgaaag agatggaaga gtgtcaattg
      721 cggccttctc acctcgaagg gatggaggag ggagaatgga ttctcattga ctatggtgat
      781 ttaatgattc atatttttcg agaaggagaa cggttatttt atcagctgga aagattgtgg
      841 catagagctc cccttcttta ttcagaaaaa gaaggtttaa atcttttgga ggaaattaaa
      901 gattgattga atttctttct ctattgtggt ttaatgtaaa agtttggttt taaacgaacg
      961 aattctctag aagaggaatt aaattaataa aatcttttct ggcgatggaa gggaggtaac
     1021 attatattgg agaagagata tcaaattact tctgaatcag ttactgaagg tcatccagat
     1081 aaaatctcgg atcaggtttc tgatgccatc ctggatgcta tattagctca agacccccag
     1141 ggaagagtag cagtagaaac gctggtagct acgggcttaa ttttaattgc cggtcaagtc
     1201 agcaccagtt cttatgtaga tattcctcat gttgctcgaa gcacagtaag agatataggt
     1261 tatacccggg ctaaatatgg ttttgacttt gaaacctgtg cagttttaac tgctatagat
     1321 gagcagtctc cagatattgc tctgggagtt aatcgttcgc tggaagtgaa aatgggagaa
     1381 gaagtagagg atgaagagct ttctttaggt gctggagacc agggtatgat gtatggttat
     1441 gcttgtaatg aaactccgga acttatgcct tttcccattg ttctggctca ccgcctggct
     1501 tttcgcctgg cagaagtaag aaaaaagggg gttctttctt acttaagacc tgatgggaaa
     1561 acacaggtta ctgtggatta tgtgaatgat aaaccagtaa ggattgacaa tatagtggtt
     1621 tctgctcaac atcatcctga catttcttta gatactttgc gggaagacat attgagagaa
     1681 gtaataaatc cagtggttcc ttcttcttat agagacgata aaacccaggt tttagttaac
     1741 ccgacaggta ggtttgttat tgggggacca cagggggata ctggtcttac gggaaggaag
     1801 ataattgttg acacctatgg aggcattagc aaacacggag gaggatgttt ctctggtaag
     1861 gatcccacta aagtagaccg ttctggtagc tatgccgctc gttatgtagc taaaaatgtg
     1921 gtagctgccg gtttagctga taggtgtgaa ttccagttag cttatgctat aggtgtggct
     1981 cgtccagttt ctatgagcat agatactaaa ggaacgggga aaattcctga tgatgaaatt
     2041 cttcgcctgg tgaaggaggt ttttgatctc cgccctggag ctataattaa aaatcttaaa
     2101 ctgcggcgac ctatttacaa aaagactgct gcctatggtc attttggacg taatgaccct
     2161 gacttcagct gggaaaaaac tgataaagcc gatgttttaa aagaaaaagc gggattatga
     2221 gaggaggagg gagtatggaa tttgaagtag ctgatttaga tttagccagt gagggaaaaa
     2281 agaaaataga gtggacagaa cgagagatgc cggtggttac caacattgct tcttcatgga
     2341 aagatgtttt gcctcttcag aacttaagaa tagctgcctg tctccatgtt accgctgaaa
     2401 ctgcagtttt agtgagagct ttacaggcag gaggcgcaga agtgcgactc tgtgcttcta
     2461 atcctttaag tacccaggat gaagtagcag cttccctggt aaaggattat ggtatttctg
     2521 tttttgccat tagaggagaa gatcacgata cttattatca acatattcaa aaagtactgg
     2581 aattcaaacc tcatattacg atggatgatg gggcagacct ggtttccttt gtacatgttc
     2641 acaaaagaga tcttctttct gaaattaaag gtgggacaga agaaacaacc accggagtta
     2701 ttcgactgag gagtctggag aaaaatggag aactgggtta cccggttatt gctgtaaatg
     2761 atgctctcac caagcacctt tttgataatc gctatggaac agggcaaagc actatcgaag
     2821 ctattttacg agctactcac attttattag ctggtaaaaa agtggtggta gcaggttatg
     2881 gatggtgtgg gaggggaatt gctgctcgag ctaaaggcat gggagctcga gtagcagtgg
     2941 tagaagtgga tcctgtaaga gcactggaag cgttgatgga tggtaatgat gtattatcta
     3001 tgaaaaaagc tgcttcctgg ggagaagttt ttatcactgc taccggaaat acttcagtta
     3061 ttcgaggaga acatttctta tctcttccag atggagctat tttagctaat gctggtcatt
     3121 ttaatgtgga gattgatgtg aaagagctgg aagaaataag tgtaaacaaa agagaagtac
     3181 gtcctttagt ggaggaatac actttgggta atggaaaaaa agtatatctt ctgggagagg
     3241 gacgtctagt caatctgacc tgtgcggaag ggcaccctcc tacagttatg gatatgagtt
     3301 ttgccaatca ggctctgtca gtaaaatatt taaaggaaaa tgcttctcat ctcgagaaaa
     3361 aagtttatcg agttcctgaa gagatagacc gggagatagc ttccctgaag cttcaatccc
     3421 tcggtttaga aattgaaaaa ttgacttctc gccagaggga atatctttct tcctggcaag
     3481 aaggtactta ataaatttcc ctgtttcacc aaaagtgaaa ccaaaaaaag tccagaggga
     3541 ggaatgaaca tgaataatta cgaactggga gtgatactcc gacctactct ttctgaggaa
     3601 gaagtaaatt cagcagtaga aaaactgaag aattttatcg aagagagagg cgaggtagta
     3661 aaagtagatt tgtggggaag aagaaagctg gcttacccta ttcaaaaata tcaggaaggt
     3721 tattatgtat tcttcaattt taagtttccc ccttcagaag tggcggaact caaaagatgg
     3781 atgggtttaa acgaggaagt tatccgccat cttttggtta aagaagatga aaaagaggaa
     3841 acttctgttt cttcatcttc ttctccggaa gaggagattt cagaagaggc agtgagcgaa
     3901 gacgcggctg gtgatgtctc atctcagtct taatcgagtt tttttaatcg ggtttttacc
     3961 tcacagtccg gtattgcgct atgttccatc cggtatacca gtggttaatt tttcgctggc
     4021 agtgtaccga actttttcct ctgaagaaga aaaagaatgg atagattatc tcaacataat
     4081 agcttggcga gacctagctc ttttctgcgg ggaaaatcta aaaagaggag actgggtttt
     4141 tgtagaagga aggattcagg taagagctta tgaaagctca gagggtaaaa aaagaaaagc
     4201 ttatgaaatt gtagcttcag aggtgaaact tttatcatat ccgaagaata gaaaaagaga
     4261 ggaggtaact gaaaatggca gtagatagag atgaatactc gggacgaaag aaattctttc
     4321 gtaggaaaag gagaccttgc atgttttgtt tgggtaaggc aaaaatagat tataagaacg
     4381 ctgattttct tcagaggttt ttaagtgaaa gaggcaagat tgttcctcgt cgagttacgg
     4441 gcacttgcgc taaacatcag cggaaagttg ctcgggctat aaaaagagcg agagagcttg
     4501 ctcttttgcc ttatgcagta gattaggatt gatgagacaa gccagatcca cggtggaggg
     4561 agcttttctg gctgccctca ccgttatttt ttatttatct tcgatatata tccctgtttt
     4621 aggtttttta ttgagctttc tctgtcctct tccaattatg tttttaacca ttcgctggga
     4681 tatgaaagcg gggttgctgg cggctttggt ggctagtttt atagttttta tctttgccgg
     4741 cgccatatct gctttaacct gttttgtagg gtttactttt ttgggattaa ttatgggtct
     4801 taccatcaaa agaggttata actttataga agtaatgggg ataaatactc tggtttctat
     4861 ttcctctaag ttgttattaa ttggcttagt atttttaatc atgggtcaaa accctctttc
     4921 agaaactctg tcttttttag aagaaggttt gaaaaagggc ctatcttttt ttcctcagga
     4981 gggaggtata gatgtagaga tgatagccag ttttatccat atggttttac ctgctgcagt
     5041 ttttattgcc tctctttttg acacggtact caattttctt ttaggtagct ggataggaaa
     5101 gagattggat attaaatttc ctccctttcc tccttttgag aatttgcaac tgcccagatc
     5161 tattttttgg gcttttgctc tgggatggct tttcgttctg tttggaggag ctactttttg
     5221 gggcaagatt ggtctaaatt tacgaatggt tacccaggtg ctttttttag ttcaaggggc
     5281 atccgtggta tactattttc tgggtaagta tattcgttct cgattctggt taacaattat
     5341 agtagttttt gtggctttgc agccactgct ttctaccata ttgagctggg tgggagtttt
     5401 tgatttttgt tttgatttgc ggaaaattag agggaagtga ggaggatata tatgaaagtg
     5461 atacttttgc aggaagtgga aaatttagga aaagaaggag acatggtaga agtaaagaac
     5521 ggttatgctc gtaattatct tttgcctcga aaattagcta tagaggctag caagggtaat
     5581 attacccaga tagaagcact gaaaaagaaa agagctattc gagaggctaa ggagttagaa
     5641 gaggctaaaa tactacagga aagaatagat ggtttggttc ttgagttcga aaagaaagca
     5701 gggaaaacgg gaaaactcta cggatcggtt acctcccggg aaatagcgga gaagatttct
     5761 caggttgtgg gcataaattt agatcgaaaa tatctggaac tgggagaagc tctcaaagaa
     5821 ataggtgagt atccggtgaa agttaacctg ggtaaaggca tttacgctac agttcaagta
     5881 aaagttcagg gagaagaagt ggaggaggag gcggaagagt cctgagtatc gagagggttc
     5941 cgccccatga cctcgaggca gaacaagcta ctctggggtc gatgcttctt gataaagaag
     6001 ctcttctacg ggtgttagat attttgcaac cggaagactt ttacagggat aatcatcgca
     6061 ttatttttga aagcattacg gagctttttg aagaggacca ggcctgtgac attgtaactc
     6121 tggttgccaa attaaggcaa aagaatcagc tggaggctgt agggggcata gattatttaa
     6181 ctcaactggt aaacattgtg cctacagctg ctaacgctga gtattatgct catattgtag
     6241 aagagaaatc catatttcgc tcagtaatta acctttgtac cagaatcatc cgggaaagct
     6301 atgatgcaga cctggatgct aaagaacttt tagaccgagc ccagaactgg attttacaac
     6361 tttctcaaaa gaagattcgc tatgattttg tgcctctcaa ggtcataatt aacgaggctt
     6421 ttgatcgaat tgaggagttg tatcatcgag atgagcacgt tactggagtt ccttctggtt
     6481 ttgtagattt agacttgatt actgctggtt ttcagccttc tgattttgta gtagtagcag
     6541 cccgtcctgg tatgggtaaa accagtttat gcttgaatat tgctcaatat gtagggacca
     6601 aaaaagacat ctctgtggct atttttagtc tggagatgtc caaggatcaa gttgctcaga
     6661 gaatgctctg tagcgaagct cgaattgacg cggcaaaagt gagaaaggga tttatagcgg
     6721 agagggaatg gccggaattg gcccgggcgg cagggagatt agctgaagca aatatttttg
     6781 ttgatgacac tcccagtatt tccgttctgg agttgagagc taaagctcga cgtcttaaag
     6841 cagagaaagg tttaggttta gttatagttg attatctcca gcttatgaga ggatacggtc
     6901 gcccggagaa tcggcagcag gaaatttcgg aaatttcccg ttccttgaaa gccatggcga
     6961 gggaattaaa tgtgcctgtc atagcagttt ctcagcttag tagagcagtg gaacagagaa
     7021 ctcctaagag gcctcagctt tctgatttac gggaaagcgg ggctatagag caggatgctg
     7081 atttagttct ttttatttac cgggaagatt attataatcc taacacccct aaaaagggaa
     7141 ttgctgaagt tattgtagct aaacagcgca atggccccat aggaacggta gagctgatgt
     7201 ttcagaaaga ctatgctcgt tttgaaaatc tggcccgata attttcaaaa aatagggtaa
     7261 aaaaggaact atttattaaa ctctggatat aaaaaggttg aacctcggag tgtttccctc
     7321 tttaaacccc ctccttagtt aaagaacgct ccggggttca acagaatatt aagattggga
     7381 gggtgggaaa tggacaagct cactatatat caaagacctt tattatcaaa tccttctctt
     7441 ttagtagggt ttactggttg gatggatgga ggagatgttt ccagcggttc ggtggcctat
     7501 ctccaaaaaa aatttgcagc agaaatgata gcagaaattg acccccagga gttttatctg
     7561 tttaattttc ctggaagcat ggaagaggta gcccagtttc gcccttacac tttgattaaa
     7621 gagggaataa taaccgaatt tcactatccc cagaaccaat ttttctgtgc ttcaaaggaa
     7681 aaccttcttc tctttctggg taaagaacct aacatgaagt ggaatgagta tgtagagaat
     7741 atcttttttt tgggaaaaga atttggagtg aagagaattt atttcatagg tagtgttagt
     7801 gggttgattc cccatactcg agaaccgcgg gtaacttgtt ccgtttctga tgaaagatta
     7861 aaaaaagaac tggaaggcta taacctttat ttcaatgatt acgaaggtcc agccagtatt
     7921 accacccttc tcactcttat ggctcctcaa aaaggagtag agatgattaa cttggtggtg
     7981 gaaataccta tgtatatcca ggctactaat cccaaaggta tttcttctgc tttaggaatt
     8041 ctttcttctc ttcttaactt gcctttagta ggagaagacc tggtaaacat gagtgaagaa
     8101 tttgatagaa acgtagaaca gttagtaaag aagtatcctg acttagtaga gcgaatcaaa
     8161 aaattagagg agaattacga tcgagaaatt ttagaagacg accagaattt tcgagaatgg
     8221 ctcagaaggc acggtattga taaaatttga gaaagataaa gctgaaaaaa aggtagtaca
     8281 tctcatcaca gcatcccgca aaacgcaaaa tctattgtga cttatggtta taagggggct
     8341 ttgtccccta taaccctctt tctcatccgt tgtccccgag aggctctatc aggaatccag
     8401 aattatagag aaaaaaactc ccctttgtta ttttccaaat acacaaaaaa ggcaaaatat
     8461 aaaaagctat tgaggaaaga aaaacaattt cctcagtgct ccggtgtaaa cataaaacaa
     8521 agagacaaaa agataaagaa ctattcct
//
LOCUS       DAOPSS010000068         8212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_7291_length_8212_cov_9.947407, whole genome shotgun sequence.
ACCESSION   DAOPSS010000068 DAOPSS010000000
VERSION     DAOPSS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7291_length_8212_cov_9.947407"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PKN31_05255"
     CDS             <1..588
                     /locus_tag="PKN31_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOA99365.1"
                     /translation="DFPSHKIITSSQATIFYLSQMNPHPRVYLLGTPELEIEFLQANF
                     ILVQKDPDFVVLGFDTGLTYQKINQACQFIRSGVPFFATHPDLNCPTEEGPVIDAGSF
                     IKAIEASTDRSPKIFGKPYPEMIEYILQRTEAKRGEVAIIGDRLYTDIAMGKKAGITT
                     ILVLTGETKEEDVKNSPWQPDFIFTSLEEINNHLK"
     gene            599..1927
                     /locus_tag="PKN31_05260"
     CDS             599..1927
                     /locus_tag="PKN31_05260"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HOA99366.1"
                     /translation="MPDKLFFFAEATTFPCKCGKIHHIPTKKTMLGKEVLPRIPQTLS
                     ELNLGKKLLLLFDNNTYEAAGKETERILKKASFEVVPVLLEKPPELKYLEPDEKARER
                     IGSFLFHKPDFILAVGSGVINDLAKFVAHRTQIPYAVVATAPSMDGYSSPGAPMLVGG
                     YKVTYDATPPCAIFMDLEILSQAPLPLIQSGFSDLVGKITANADWVMRNILQEEYFCE
                     YTWELVKGALEILREKAEKIGEREEEAIYALAVALLNSGFSMTMVGDSRPASGAEHLV
                     AHHLEMMALHRRMDPSLHGLRVGGATVIIKKMYDRFIQELKEIDWEKSKNHSFRSQEE
                     ELKIHFGPLFPFIEKDAKEKLKWDKEEIKVADSAFLSQQLQDKLSVIPDPASLLQRAQ
                     APQTLKELGFSEKMIREALLFSRFIRKRVTILDLLGKAGLLEEYIDYALS"
     gene            complement(2298..2912)
                     /locus_tag="PKN31_05265"
     CDS             complement(2298..2912)
                     /locus_tag="PKN31_05265"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOA99367.1"
                     /translation="MKKENWPKWFIIVSVFSLLLGVTGCSDYLPLPTETPDPSPTDTP
                     TPTDTPTPTPVDTGINFTLPDLDGKEVSLLEFRGKPVLVFFFKSYCSYCKEEAPAIQD
                     IYRRHQGELTVMGIAVNESGTSGNLIAPTEEYTQMIRSGFVNSSDWTFPVLIDDYGKV
                     QRECFTRLGVPAFLFLNEKGEIQHTVTGAVSKNELESLLYQYLL"
     gene            complement(2975..5071)
                     /locus_tag="PKN31_05270"
     CDS             complement(2975..5071)
                     /locus_tag="PKN31_05270"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA99368.1"
                     /translation="MPKKENKSSLNLPLLKKGVLIALFGLVLLSPYYRGLYFDYERFL
                     FFAVFWGIAIFFFVAQYFLRQERIIISTIPEWIFIFLVLLYLLNIPLAADKGGAFREF
                     FNYLTFAMFFLLVENLWSSLEDKKWFLLALGLNGIILAFLGIFYQFGWVSPSATPLGM
                     SMRDLFIGGRLHSTFQYPNTTAAYFAMSYIALLTLFLLEKKKSWFLGAIFLSFLSLSG
                     FFFTYSRGGILTLPLALIFLFLLLPRREKIQLAEVLFFSLLVLLLFVSPLENYLVHKD
                     PLFFGFLFSGALILTGVIFLLSKVEEKVASLSNRSLLLGVVSLVGLVVIIFLLALYSD
                     FLPPQLSRRLRDISFTTHSAAERFIFYRDAWRVSFPRLLNGWGGRAWKALYLGYQSAP
                     YFTESTHNFYLQILVEGGLIGLSLIAILLFSLFYPFIKNRHSLPSPDKILIFGVLSLL
                     FMGFTHSFLDVNFSLGAYHFTIWFFAAGVANYLRDYKFSSFPLSFRIHYEFLILLGVV
                     FFVLSILIFQGTNNKIWGDYYAKAGDNDSALYFYQKAARFSFGDAEIHLALSRAWREK
                     FLKEGNPQFREKSKKEAERAHRLSPFYYLYLEQLGVLYVEEGQFEKGLQFLEEAVEKA
                     PLLSSTYEVLVSAYRAVGDFYKSRGEEEKAREYYARGLLVREKFQKNASHSRRNITES
                     ENLQEAFQEFSLLLEN"
     gene            5223..5399
                     /locus_tag="PKN31_05275"
     CDS             5223..5399
                     /locus_tag="PKN31_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99369.1"
                     /translation="MVIFFLRALLSFVAEIITGDFIPGCRRYLYHLPVEISPFLSPSG
                     KYLRNNVIPENSTK"
     gene            5516..6007
                     /locus_tag="PKN31_05280"
     CDS             5516..6007
                     /locus_tag="PKN31_05280"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HOA99370.1"
                     /translation="MNSIQIKPLTYENFQKYGDFVRLDQVSPEKYQRIGQPPVEFYPD
                     LILQNLGSPILGVSLCRVFPRDLKVELTEHHNYTEEGNLPLDGDVYMHVGLATQEVEE
                     MEVEVFEVPKLTFVRLKIGVWHHALYAKGDKPVNVLVLLAERTYHNDCEVKPCNFTFG
                     EKR"
     gene            complement(6142..8154)
                     /locus_tag="PKN31_05285"
     CDS             complement(6142..8154)
                     /locus_tag="PKN31_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018930575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOA99371.1"
                     /translation="MFFKQVGNRLIAQKDGETIWLEPFGKDCLRFRSTLNKEINEENW
                     TLLSQPTIPALIDIQPMKATIKNGKIMAEILADGTVNYFTDGGKILLEELWIDGRVSN
                     ADVLKARNYKALSSSLYRTDLYFKAYEGERLYGMGQYVNGCLDLKGCVLELAQKNTQI
                     SIPFLVSTRGYGFIWNNPAVGRAELAKNHTLWHAKAGKQIDYLVIAGDTPVDIVKKYT
                     DLTGKPPMLPEWALGFWQCKLRYRTQEELLNVAREYKKRELPLSVIVIDFFHWPHQGD
                     WKFDSEYWPDPQAMVDELEKMGIKVMVSVWPTVEINSENYLEMVKRGFLVRTERGVPT
                     IFTFRGINTHFDATNPEAREFIWEKVKENYYKYGIKMFWLDVAEPELGMPGFGYDNIL
                     SYDYDNLRYHLGNGLEVSNIYPFYYAQAFEDGLRLAGEKEIVNLIRCAWLGSQRFGVV
                     VWSGDIPSTFDSLQKQLRAGLNMAMCGIPWWTTDIGGFFDGDPNDPAFQELIIRWFQF
                     GVFCPIFRLHGFRLPYPPDSNKCEPYDLTGGPNEIWSFGEEAYDIIKNLLFLREKIKP
                     YLMEQMKRAHEEGIPIMRPLFIDFPSDDEAYDVEDEYMFGPDLLVAPILYEGMRSRRV
                     YLPEGAKWTSAYDGTVHEGGQWIESEAPLNVIPVFLKNEAKIPISF"
BASE COUNT         2697 a         1693 c         1489 g         2333 t
ORIGIN      
        1 gatttccctt cccataaaat aattacctct tcccaggcta ccatttttta tctttcccaa
       61 atgaatcccc atcctcgagt gtatctttta ggtaccccgg agttagagat tgagtttttg
      121 caggctaatt tcattctagt acaaaaagat ccggactttg tggttttagg atttgataca
      181 ggcctgactt atcaaaaaat aaaccaagct tgtcaattca taagaagtgg agtccccttt
      241 tttgccactc accccgacct aaattgtcct accgaggaag gaccggtaat agatgccgga
      301 tctttcataa aagccataga agcttctacc gaccgatctc ctaaaatatt tggtaagcct
      361 tatccggaaa tgatagaata tattctgcaa aggactgaag ctaaaagggg agaagtagcc
      421 attattggag atcgtctcta tactgacatt gctatgggga aaaaagcggg aatcactact
      481 attctggttc tcaccggaga gacaaaagaa gaagatgtaa aaaattctcc ctggcaacca
      541 gatttcatct ttacttcttt agaagaaata aataatcatc tcaagtaagg agagaaaaat
      601 gcctgacaaa cttttctttt ttgccgaagc caccactttt ccttgtaaat gtgggaaaat
      661 tcatcatatc ccgactaaaa aaactatgct ggggaaagaa gtgctacctc gcatacctca
      721 aacattatcg gagcttaatt tagggaaaaa acttcttctc ctttttgaca ataataccta
      781 cgaagcggca ggaaaggaaa cagaaagaat tttaaaaaaa gcttcttttg aagttgtacc
      841 agtcttactg gaaaaacctc ctgagctaaa atatttagag cccgatgaaa aggctcgaga
      901 acgtatcggt tcttttcttt tccacaaacc tgattttata ttagcagtag gctcaggagt
      961 gataaatgat ttagctaaat ttgtagccca tcggacacag attccttatg ccgtagtagc
     1021 tactgctcct tctatggatg gatattcctc tcccggagct cccatgttag tgggaggata
     1081 taaagtaacc tatgatgcca ctcctccttg tgctattttt atggatctgg aaattctatc
     1141 tcaagctcct cttcctttga tccagtctgg tttctccgat ctcgtgggta aaatcactgc
     1201 caatgctgac tgggtaatga gaaatatttt acaggaggaa tatttctgtg aatatacttg
     1261 ggagctggta aaaggagcct tagaaatatt aagagaaaaa gcagaaaaaa taggagaaag
     1321 agaggaagaa gccatctatg ctctggcggt agcgctcctt aactcgggtt tcagtatgac
     1381 tatggtggga gattctcgtc ctgcatcagg cgcagaacac ctggttgctc accatcttga
     1441 gatgatggct ctgcatcgga gaatggaccc ttctcttcat ggcttgaggg taggaggagc
     1501 aacagtcatc attaagaaaa tgtatgaccg atttatacaa gagctgaaag aaatagactg
     1561 ggagaagagt aaaaaccatt cttttagaag ccaggaagaa gagttaaaga ttcactttgg
     1621 cccactgttt cctttcatag aaaaagatgc taaagaaaaa ctgaaatggg ataaagaaga
     1681 aataaaagta gcggattctg cctttttatc gcagcaattg caagacaaac tatcagttat
     1741 ccctgaccca gcttctctat tgcaaagagc tcaagctcct caaaccttaa aagaattagg
     1801 attctcagaa aaaatgatta gagaagctct acttttttct cgtttcatac gaaaaagagt
     1861 aaccatcctt gacctcctgg gaaaagcagg acttttagaa gaatatatcg attacgccct
     1921 atcctgacgg tttttgtaat taactataaa gcgcggggag ccattccttt tttctgcttt
     1981 cctgcagagg atcgtggcaa caagcaaatc atatcactcc actgagggag ggcaagtgcc
     2041 tccctcagtt gtttttctat ctttcgtatt ttatcttttt tctttttcag ggatttataa
     2101 gggagcatca ctcccttata aatctattat ctgcccgcta cttgtataac aggaaagagg
     2161 aaaaactcct cacgaaaaag aaaaaggagt ccctcgcaaa aagaagaggg gttataagaa
     2221 gtttacccct atagttctat ttaagaaatt agcaagaaac acagataaag atggacaaat
     2281 tgctatctca ttcatattta taagagatat tggtataaaa gagattccaa ctcgttcttt
     2341 gaaacagcgc ctgtcacagt atgttgaatt tctccttttt cgttcaagaa aagaaaagct
     2401 ggaactccca atcttgtaaa acattctctt tgcacctttc cataatcatc gattaaaacc
     2461 ggaaaagtcc agtcactact attaacaaaa ccgctcctga tcatctgggt atattcctcg
     2521 gtaggagcaa ttaaatttcc agatgtcccg ctttcattca ctgctattcc catcaccgtc
     2581 aactctcctt gatggcggcg ataaatatcc tggattgcag gagcttcctc cttgcaataa
     2641 ctacagtaag acttgaagaa aaatactaaa actggttttc cccgaaattc aagaagggaa
     2701 acctctttcc catctaagtc aggaagagta aaattaattc cagtatctac tggcgttgga
     2761 gttggtgtat ctgtaggagt tggtgtatct gtaggtgaag gatcaggagt ctctgtgggt
     2821 aaaggtaaat agtcagagca tcctgttact cccaaaagaa gagaaaaaac tgaaacaatg
     2881 ataaaccact tcggccagtt ttcctttttc atagtttaag ctcctctctt atcatcttat
     2941 tcttttaatg tccaattata caacataacc acattcaatt ttctagcaat aaagaaaatt
     3001 cttgaaatgc ttcttgcagg ttttctgatt cggtaatatt tctccgagaa tggctggcgt
     3061 ttttttggaa tttttctcgc accaataaac ctcgagcata atattccctg gccttttctt
     3121 cctctcctcg agatttataa aaatcaccca ccgctcgata tgcagaaacc aaaacctcat
     3181 aagtagaaga aagaagagga gctttttcta ctgcttcttc taaaaactgc aagccttttt
     3241 cgaattgacc ttcttccaca taaagaactc ccagctgctc taaataaagg taataaaagg
     3301 gagaaaggcg atgagctctt tctgcttctt ttttgctttt ttctcggaat tgaggattgc
     3361 cttcttttaa aaacttttcc ctccaggctc tgcttaaagc caggtgaatt tctgcatctc
     3421 caaaactaaa ccgtgctgct ttttgataga aataaagagc gctatcgtta tctcctgctt
     3481 tagcatagta gtctccccat attttattgt tggtgccttg aaagataaga atggagagaa
     3541 caaagaaaac cactcctaat aatattaaaa actcgtagtg aattcgaaaa gataagggaa
     3601 aagaggaaaa tttgtagtcc cgaagataat ttgccactcc tgcagcaaaa aaccagatgg
     3661 tgaaatgata tgctcccagg gaaaaattga catccagaaa actatgagta aatcccataa
     3721 acagcaaaga taaaaccccg aaaatcaaaa ttttatctgg ggaaggtaaa gagtggcgat
     3781 tcttaataaa aggataaaaa agagagaaaa gcaaaatagc aatgagagaa agccctatta
     3841 gccctccctc taccagaatc tgcaaataaa aattgtgagt actttcagta aagtaaggag
     3901 ccgactgata acctaaataa agagccttcc aggctctccc tccccatcca ttaagaagac
     3961 gaggaaagct gaccctccag gcatctcggt aaaaaataaa tctttccgcc gcactatggg
     4021 tggtaaaact gatatctcgt aatctccgag aaagctgagg aggtaaaaaa tcagaataga
     4081 gggctaaaag aaagataatt accactaacc cgaccagact tactactcct aatagaagag
     4141 aacggttgct aagagaagca actttttctt ctactttgga caaaagaaag ataactccgg
     4201 tgagaattaa agctcctgag aatagaaagc caaaaaagag aggatccttg tgaacaagat
     4261 aattttctaa aggggaaaca aaaagcaaaa ggacaagaag agaaaagaaa agcacttctg
     4321 ccagctgaat tttttctcgg cgagggagaa gcaaaaacaa aaaaattaaa gccagaggga
     4381 gagttaatat ccctcctcga gagtaagtga aaaaaaagcc agaaagagag agaaaagaaa
     4441 gaaaaattgc tcccagaaac cagcttttct ttttctccag aagaaacaga gtcaaaagag
     4501 caatgtagct catagcgaaa taagcagcag tagtgttggg atattgaaaa gtggaatgca
     4561 atctgccacc aataaataaa tctctcatac tcatacccaa gggggtagca gaaggagata
     4621 cccagccaaa ttgataaaaa atacccagaa aagccagaat aataccattt aatcccagag
     4681 caagaagaaa ccacttttta tcttccaggg aagaccacag gttttctacc agcaggaaaa
     4741 acattgcaaa agttaaataa ttgaaaaact cccggaaagc tcctccttta tcagccgcca
     4801 ggggaatatt aagaagataa agtaaaacta agaaaataaa tatccattct ggtatagtgc
     4861 taataattat tctctcctgg cgtaaaaagt actgagcaac aaagaaaaaa atagctattc
     4921 cccagaaaac ggcgaaaaag aggaaacgtt cataatcaaa atacagacct cgataatagg
     4981 gagaaagaag caccaggcca aataaagcta ttaaaacacc ttttttaagg agaggtaaat
     5041 ttaaagaact cttattttct tttttaggca tattcagtta atatttcaaa catatccttt
     5101 aagttattac tgatttatct tcaattatag caaacaatat tacttcctga ctttcctgaa
     5161 agtaaaaaaa tcaaaaatat ttttcagcaa aatcaagcat agtgaaccac aaaatctaat
     5221 ctatggtaat attttttctt cgtgctctct tatctttcgt agcagagatt attacgggcg
     5281 atttcatccc ggggtgtcgt cgttatctct atcaccttcc cgtagaaatc tctccctttc
     5341 tttctccatc gggaaagtat ttgcggaata atgtaattcc agaaaatagc acaaaataaa
     5401 tgaaaataac aaaggaaaaa ataccaaaac tttcatggtc actaattgac cggatataaa
     5461 agactgttac aataaaaaag aggaattttt attatttacc agaggagaga agattatgaa
     5521 tagtatccag attaaaccac tcacttatga aaacttccag aaatacggtg attttgtacg
     5581 gctcgaccag gttagtcctg aaaaatatca gcggattggt caacccccgg ttgagttcta
     5641 tcccgacctg atcttacaaa atttaggtag ccctattctg ggagtgtctt tatgcagggt
     5701 tttccctcga gatctgaagg ttgagctcac cgaacaccat aattatactg aagagggaaa
     5761 ccttcccctc gatggagatg tgtacatgca cgttggactt gctacccaag aggtagaaga
     5821 aatggaagtc gaagtttttg aagtacctaa acttaccttt gtccgactaa agatcggggt
     5881 ttggcatcac gccctctatg ccaaaggaga taaaccagtt aacgttttag tattattagc
     5941 agagagaact taccacaatg attgcgaggt caaaccttgc aatttcacct ttggagaaaa
     6001 gagataaaat tactaacgag aatagatttg gccgggtgaa acagaaatat tactcggcca
     6061 aatctattct taaaagtcac acatgcttca ttatttcccg tggttctatt ctatgctatc
     6121 tttcccggaa agatttgctg gtcaaaacga aataggaatt tttgcctcat tctttaaaaa
     6181 tactggaatc acattcaatg gtgcttcgct ttctatccat tgaccaccct catgcactgt
     6241 gccatcgtaa gcgctggtcc attttgcccc ttcaggtaaa tatactctcc tcgacctcat
     6301 gccttcgtat aaaataggcg ctacaagcag gtcaggacca aacatatact catcttctac
     6361 atcatatgct tcgtcatcac tgggaaaatc aataaataag ggtctcatta ttggtatccc
     6421 ctcttcatga gctcttttca tttgttccat taagtaaggt tttattttct ctcttaagaa
     6481 tagcagattt ttgattatat catatgcttc ttcgccaaat gaccatatct cattaggacc
     6541 accagttagg tcatatggct cacatttatt cgaatctgga ggataaggga ggcggaaacc
     6601 atgcagcctg aaaatggggc aaaatacccc aaactgaaac cagcggataa tgagctcttg
     6661 gaaagcaggg tcgtttgggt ctccatcaaa aaaaccgcct atatcagtag tccaccaggg
     6721 gataccacac atcgccatat ttaagcctgc tctaagctgt ttttgtaaag aatcaaaagt
     6781 agaaggaata tctcccgacc acaccactac tccaaaacgc tgactaccca gccaggcaca
     6841 gcggataagg ttaacaattt ccttttctcc tgctaatcgc aaaccatcct caaaggcctg
     6901 cgcgtagtaa aaaggataaa tgttgcttac ttctaagcca ttacctaaat gatagcgaag
     6961 attatcataa tcataggaca aaatattatc gtaaccaaaa cctggcatcc ccagctctgg
     7021 ttctgctaca tccagccaga acatctttat accatactta taataatttt ccttaacttt
     7081 ctcccagata aattctctag cctcagggtt agtagcatca aaatgggtgt tgattcctcg
     7141 aaaagtgaaa attgtaggaa ccccacgttc cgttctaacc aagaaacctc tctttaccat
     7201 ctctaagtag ttttcactat ttatttctac tgtcggccag actgatacca taactttaat
     7261 tcccattttt tctaattcat ctaccatagc ttgcgggtct ggccaatatt ccgagtcaaa
     7321 tttccaatct ccctggtgag gccaatgaaa aaagtcaata actataactg ataacggtaa
     7381 ttctcttttt ttgtattccc gagctacgtt taaaagctct tcttgagttc ggtagcggag
     7441 cttacactgc cagaagccca aggcccattc cggtagcatt ggtggtttgc cggtcaaatc
     7501 agtatacttt ttaactatat caaccggagt gtctccagca ataacaagat agtcaatctg
     7561 ttttcctgct tttgcatgcc acaaggtatg atttttagcg agttctgctc taccaactgc
     7621 cgggttattc cagataaatc cataccctcg agtagaaact aaaaaaggaa tactaatctg
     7681 ggtatttttt tgagctaatt ctaatacaca tcctttcagg tctaaacacc cgttgacata
     7741 ctgtcccatt ccataaagac gttctccttc ataagcttta aaatacaagt ccgttctata
     7801 caagctactg ctaagagctt tataatttct agcttttaaa acatcagcat tgcttaccct
     7861 tccatcaatc cacagttctt ctaataaaat ctttccacca tcggtaaaat agttgactgt
     7921 tccatctgct aatatttcag ccataatctt tccatttttg atagtggctt tcattggttg
     7981 aatatctatg agagctggaa tagtaggttg agatagtaga gtccaatttt cttcattaat
     8041 ttctttattc aaggtagacc taaaacgtaa acagtctttg ccaaatggtt caagccagat
     8101 agtttcacca tctttttgag ctattagcct gtttccaact tgtttaaaaa acatcttatg
     8161 cactcctttc aaggttatac tatttttagt tgaaattgaa aagagtggct cc
//
LOCUS       DAOPSS010000069         8122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_7378_length_8122_cov_7.866989, whole genome shotgun sequence.
ACCESSION   DAOPSS010000069 DAOPSS010000000
VERSION     DAOPSS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7378_length_8122_cov_7.866989"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PKN31_05290"
     CDS             complement(<1..1356)
                     /locus_tag="PKN31_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOA99372.1"
                     /translation="MTDKIFRWIGLISTIGLGSLLIMGFPGSQIMANHVENLSPDFIK
                     GVDISMLYEIEVNGGKFYDNGIEKDALQILKDYGVNWIRVRIWNDPTDENGKPLGGGN
                     CDYIKMTELARRAKTLGMKVLVDFHYSDWWADPGKQNKPDSWKNLEGASLATAVADFT
                     YEVLKYMEDNDALPDMVQLGNEVNNGFLWPDGKIAGEGGFDSFVTLFNAGAQAVRKIS
                     KDTKIALHLAEGGNSDLFEWFLGNVTTRGIDFDVIGISYYPYWHGTLEELKSNLNNIE
                     KYDKEIAIFETAYAWTLEDADGHPNIFGPDMEITGGYKATPRGQATAIREIMEAVAQV
                     TSGRGLGIFYWEGCWIPVKGAGWKFGEGNPWESQALFDFKGHALPSLRVFNLIYEEDY
                     IEPEFREIISPLNIKIFLGESPALPEKIKILFSDDSIRSLPVKWEHIEEEFLTSPGEF
                     QV"
     gene            complement(1359..3797)
                     /locus_tag="PKN31_05295"
     CDS             complement(1359..3797)
                     /locus_tag="PKN31_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOA99373.1"
                     /translation="MIEKRRPFLTHIILWILIPVILFPIIWIVSTSIRRDEAIFSTKL
                     FSSRVSLQNYRDLIAPEKNIPALVQEMQSLILRTSPYDSWEEKRLKEVLLEDIEAFKG
                     YLQETRDRYERAQSSFDNINLALSSRSNNIKEEVLVSLRELKEYLEKNVPVEESNEEN
                     LKIVLYQLLYPESYNSKLFIYLKEDLEKVSDYHINSPEDYEKALSALEKTYQISLGSL
                     KTELAQLEKKLEENSRNYGIARTEYEKIEEEILSISQILNSEVKSEITSLLEELKAVE
                     IIKNTENKLVSLSKDTSPGAFTPPSLEVLNLLIENLSKYPDFYELSNSLREISSYLAS
                     YEETAEGNLASPYLVLENLYIETSPLLSRLEKNVNDIQEAEKNLIALKEENTLLKNQQ
                     ELLREGIKKVEETLIPATRAEQVQIFIKKIEEQIAGIEEVTKFEELVSYCVSITKDLL
                     NFVTSYIMDFGEDNLSSQIEATTNKLSWFNDYSTFSDRFNKFSKNLDTPLEKSNSLIS
                     DFEKKWADLLRASLNGEKVSVRELDELYNIIKTDYVNMVGSNISVVSRKAGDLMDTIP
                     YRELKKDLNSVDKELFRINQIWTQKTKHYFLRWVKNSIVVAGVAALITTFVCSLAAYP
                     FSRMRFWGRKYGILAMLLIQMFPSAVYMIAVYSLLNLLGKFIPLLGLDTLTGLTFIYL
                     GNIAYNVYLIKGYYDTIPSSLEESAMIDGATRFQTFYKIVIPLALPILTVVVILTFMN
                     IFNEFVFARIILQDAKNYTYAIGLWTFSSGPYQTEWGLFTAAALLGMLPMTVLFLSLQ
                     KYIVSGLTKGAVKG"
     gene            complement(3801..5510)
                     /locus_tag="PKN31_05300"
     CDS             complement(3801..5510)
                     /locus_tag="PKN31_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOA99374.1"
                     /translation="MWLLLIAFNALFIGAGVYLVQNSYYELGAILFIASFLIDLFIIN
                     PRAYPYRYTIPALIFLLLLVVYPICFTIKTAFTNYGTGHIFTRQEAVERLLYDPNYTY
                     AESEEAIGFKVFSVFEDSKPTDDFILLFDINGELYLGDVPSPSKQRGKEILLREGKTY
                     KVTSEEVSVDGKVFKLSPFPESLENINMIVSADKEYKSLYALDDPALQKNTYHFIMLV
                     QKYLANAEYLNQDQGKIIGIRVDTDGKWKFFFVERLYRLAYREIEEDGKVSQKMVIVN
                     TRTNEPLIEDKGSFYDIDENGNQVFLIGYTAWVGLENFLRIFTDKRISGPFMNIFVWT
                     IMWSLFSVLLSFLIGLVFALALNNERLRGRNVYRTLLIIPWAVPYFISVLTWKNGILN
                     ETYGILNKIILPFLGLDPVKWFNDQYWAKLACIIVNTWLTFPYMMTISLGALQSIPDE
                     LYEVAAIDGAGKWQNFRHITFPLLLTVVAPLLISSFAFTFNNFTLIYLLNSGGPPMVG
                     ATTPAGHTDILISYVYKLAFEGRGQEFGFASAISVIIFVLVGGISLLNFKISGAFEEI
                     RNE"
     gene            complement(5600..6796)
                     /gene="malE"
                     /locus_tag="PKN31_05305"
     CDS             complement(5600..6796)
                     /gene="malE"
                     /locus_tag="PKN31_05305"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HOA99375.1"
                     /translation="MKSFKFLPLILVFILVFSSISAAQTPEITIWCSEKQVDILQKLG
                     EEFKAEYGVSVNVQYVEFGSIKPNFLTAAPQGKGADIIVGAHDWVGELVINGLLEPVP
                     PFEGQETFYSTALDAFSYGGNLYGLPYSMEAIALIYNKEYVEEPPKNMEELINIAKRI
                     DATYKGEVRGFITSAAEFYYAAPFILGSGGYVFKETPEGLDVTDIGLASPGAIKGAEL
                     WKRLVDEGILTTGDNYEIMDSLFKEGKAAMIINGPWAVKAYKDAGIDYGVALIPDLEE
                     GLPAKPFVGVQGFMINAKSPNKVLARDFLVNFIATKDTMHKIYLADPRLPSREDVLSL
                     VEDDPDIVGFTQSAANGTPMPNVMEMAAVWGAMNDALNLIINGKASPEDALINAVETI
                     KAQIGK"
BASE COUNT         2440 a         1664 c         1521 g         2497 t
ORIGIN      
        1 aacttgaaac tcaccaggtg aggtaaggaa ttcttcttct atatgctccc actttaccgg
       61 aagagacctt atagagtcat cgctgaataa aatttttatt ttctctggaa gtgcaggact
      121 ttctcccagg aaaattttta tatttaaggg agaaataatt tctctaaatt ccggttctat
      181 atagtcctct tcataaataa gattaaagac ccggagggag ggcagagcat ggcccttaaa
      241 atcgaataaa gcctgacttt cccagggatt tccttcgccg aatttccagc ctgctccttt
      301 taccggtatc cagcagcctt cccagtaaaa tattcccagc ccccttccac tggtaacttg
      361 agctacggct tccattattt ctctgattgc agttgcttgc ccccggggag tggccttata
      421 tcctcctgtg atttccatat ccgggccaaa aatattggga tgtccatcag catcttcgag
      481 tgtccaggcg taggcggttt caaaaatagc tatttccttg tcatacttct cgatattgtt
      541 gagatttgat ttaagctctt caagagtgcc atgccagtaa ggataatagg aaattcctat
      601 cacatcaaag tctatgcctc tcgttgtgac atttcccagg aaccattcga ataaatcact
      661 atttcctcct tcagcaagat gcagggcaat tttggtatct ttactgattt ttctcactgc
      721 ctgtgctccg gcattaaaaa gagttacgaa gctatcaaat ccgccctcgc cagcgatttt
      781 tccgtcaggc cataaaaacc cgttattaac ttcatttccc agttgcacca tatcgggtaa
      841 ggcgtcatta tcttccatgt atttaagaac ctcataggta aagtctgcta ctgcggttgc
      901 taaacttgcc ccttctaaat ttttccagga atctggttta ttctgtttcc ccgggtcggc
      961 ccaccaatca ctgtagtgaa agtctactaa cactttcatt cccagtgttt tcgctcttcg
     1021 agccagttcg gtcatcttta tatagtcaca atttccccct cccagtggtt tcccattttc
     1081 atcggtaggg tcattccata ttcttactct tatccagttc accccgtagt ctttgagtat
     1141 ttgcagagca tctttttcta taccgttatc gtagaattta cccccattta cctctatctc
     1201 atagagcata gatatatcca caccttttat gaaatcgggg gataaattct ctacatgatt
     1261 tgccattatc tgactaccag gaaatcccat tatcagcaag ctccccaacc ctatcgtaga
     1321 aattaaacct atccagcgaa aaattttatc agtcactttt atcccttaac cgcgccttta
     1381 gtaaggccgc taactatgta tttctgaaga gagagaaata atacagtcat gggtaacatc
     1441 cccagaaggg cagcagcagt aaaaaggccc cattctgtct ggtaaggacc gctggagaaa
     1501 gtccacaagc ctatggcata agtgtaattc ttggcatcct gcaatattat tcgagcaaaa
     1561 acaaactcat tgaagatatt catgaaagtg agaatcacga ccacggtgag aataggcaga
     1621 gcaaggggta tcactatctt ataaaaagtt tgaaatcttg tggcaccatc aatcatggct
     1681 gattcttcaa gagagctggg gatagtgtca tagtacccct ttattaaata cacattatag
     1741 gcaatatttc ccagataaat aaaagtgagg ccggttagag tatcaagtcc taataaggga
     1801 atgaactttc ccagaaggtt caagaggctg tagacggcaa tcatatagac ggcactgggg
     1861 aacatctgaa tgagaagcat agccagaatt ccatattttc ttccccagaa tctcatcctg
     1921 ctgaaaggat aagcagcaag agaacatacg aaagtggtaa tcagggcagc aacgcctgct
     1981 actactatag aatttttgac ccatctcagg aaatagtgtt tagttttttg cgtccatatc
     2041 tggtttattc taaatagctc tttatccacg ctgttcaggt ctttcttgag ctctcgatag
     2101 ggtatggtat ccattaaatc tcctgctttt cgagaaacga cgctgatatt agagcctacc
     2161 atattaacgt agtcagtttt tataatatta tataactcat ccagctccct gacgcttact
     2221 ttctcaccat tcagagaggc tctgagcaaa tctgcccatt ttttctcaaa gtcggaaatc
     2281 aaggaattgc ttttctctaa aggagtgtct aagtttttag aaaatttatt aaatctatct
     2341 gaaaaagtgc tgtagtcatt aaaccagctc agtttatttg ttgtagcttc tatttgtgaa
     2401 ctcaggttat cttcgccaaa atccatgatg tacgaggtaa caaagtttaa gaggtccttg
     2461 gtaatggaaa cacagtaact tacaagctcc tcgaactttg tgacctcttc tatgcctgct
     2521 atttgttcct ctatcttttt aatgaatatc tgaacttgtt cagctctggt ggccggaata
     2581 agagtttcct ctaccttttt tatcccctcc cggaggagct cctgctggtt tttaagaaga
     2641 gtattttcct cttttaaagc aatgaggttt ttttctgctt cttgaatatc gtttacattt
     2701 ttttccagtc tgctgaggag aggagatgtt tctatatata aattctccag aaccagatat
     2761 ggtgaagcca gattaccctc tgcggtttcc tcataactgg caagataaga ggaaatttct
     2821 ctcagactat tgctcaactc ataaaagtca ggatatttgc tcaaattttc tattaatagg
     2881 ttcagtactt ctaacgatgg aggagtaaaa gcgccaggag aggtgtcttt gctgagcgac
     2941 acaagcttat tttctgtatt cttgatgatt tctacagctt tcaattcttc taaaagtgaa
     3001 gttatttccg attttacctc tgaatttaga atctggctta tagataagat ttcttcctct
     3061 atcttctcat attctgttct tgctataccg taatttcttg aattttcttc taactttttt
     3121 tctaactgag ctaattcagt ttttaggctg cccagagata tctggtaagt tttttccagc
     3181 gcagagaggg ctttttcata atcttcgggt gagttaatat ggtaatcgga aaccttttcc
     3241 aggtcttcct ttaaataaat aaatagtttt gagttataac tctcagggta aagaagctga
     3301 taaagcacta tttttaagtt ttcttcgttt gattcctcaa caggaacatt tttctccagg
     3361 tattctttta actctcggag agatacaaga acctcctctt ttatgttatt gctccgggaa
     3421 gaaagtgcta aatttatgtt atcaaaagaa ctttgagctc tttcatatct atcccgggtt
     3481 tcttgtaaat aacctttgaa agcttctata tcctcgagca ggacctcttt caaccttttc
     3541 tcttcccagc tgtcgtaggg agaagttctc aaaatcagac tctgcatttc ttgcaccaga
     3601 gcaggtatat tcttttctgg agctatcagg tctcggtaat tttgcagact cactcgggag
     3661 gaaaaaagtt tggtggaaaa gatagcctcg tctcttctta tagaagtaga aactatccag
     3721 atgataggga aaagaattac cggtatgaga atccagagaa ttatatgagt taaaaagggt
     3781 cttctctttt ctatcattgt ctattcattc cttatctctt cgaaagctcc agaaatcttg
     3841 aaattcagga gacttatccc tcccaccagg acaaatatga tgacagaaat agcgctggca
     3901 aagccaaatt cttgacctct cccttcaaag gcaagcttat aaacatagga gattaaaatg
     3961 tcagtatgac cggcaggggt ggtagcgcca accataggag ggccacctga attcagcaaa
     4021 tatatgagag tgaagttatt aaaggtaaaa gcaaagctgc ttatgagcag gggtgccacg
     4081 accgtcaaaa gtagagggaa agttatatgc cggaaattct gccattttcc agcaccgtct
     4141 atggctgcca cttcataaag ctcatcaggg atggattgca aagcccctaa agaaatggtc
     4201 atcatatagg ggaaagtaag ccaggtgttt acaatgatgc atgctaattt tgcccagtac
     4261 tgatcgttaa accatttaac cgggtcaagg cctaaaaagg gaagtattat tttattgagt
     4321 attccatatg tttcattgag tattccattt ttccaggtta aaacggagat aaaataggga
     4381 acagcccagg gaattataag gagagtccgg taaacgtttc tccctcttaa tctttcgttg
     4441 ttcagtgcta aagcgaagac caatcctatg agaaaagaaa gaagtacgct gaataaagac
     4501 cacataatgg tccagacaaa tatattcatg aaaggaccag aaatcctttt atcggtaaat
     4561 atccgaagga agttttccag tcctacccag gcagtatatc cgatcaaaaa tacctggtta
     4621 ccattttcat ctatatcata gaaagaacct ttatcttcaa taaggggttc gttggttcga
     4681 gtatttacga ttaccatttt ctggctaact tttccatcct cttctatttc tcggtatgct
     4741 aaccggtaaa gcctttccac aaagaaaaat ttccatttac catctgtgtc tactctaatt
     4801 ccaatgattt ttccctggtc ttgattcaaa tactctgcat ttgccagata cttttgaact
     4861 aacatgataa agtggtaagt attcttttgt agagcagggt catcgagagc gtaaagggat
     4921 ttgtactctt tatctgcaga tacaatcatg tttatatttt ctaaggattc aggaaaggga
     4981 gaaagtttga agacctttcc atctacactt acctcttcag aagtgacttt ataagtttta
     5041 ccttccctta aaaggatttc tttacctctc tgtttagagg gggaaggcac gtcaccaaga
     5101 taaagttctc cgtttatatc aaataataaa atgaagtcat cagtgggttt tgaatcttca
     5161 aaaacggaga aaaccttaaa acctattgct tcttcgcttt ctgcataagt gtagttcggg
     5221 tcatacagaa gtctttctac tgcttcttgc ctggtaaaga tgtgtcctgt accgtagttg
     5281 gtaaaagcag ttttaatggt aaaacagatg ggatatacaa ccagcaaaag aagaaaaata
     5341 agagcgggaa ttgtgtaccg gtaagggtaa gcccggggat tgataataaa aagatctata
     5401 aggaaagaag caataaaaag tatggctccc agctcgtaat aggagttttg aacgagatat
     5461 actcctgccc ctatgaacag ggcattgaat gctatcagta gaagccacaa aaaaaccttg
     5521 acaattttaa tttaaatctt ctccctccgg atagatttgt gagcctgaag aggtttcttc
     5581 aggctcacaa agggaagact tactttccta tttgagcttt tatggtttct acagcattaa
     5641 taagagcatc ttctggtgaa gcttttccgt tgataatgag gttgagggca tcattcatag
     5701 ctccccatac cgctgccatc tccataacat tgggcatggg ggttccatta gctgcgcttt
     5761 gagtaaagcc cacgatatct gggtcatctt ctacaaggga gagaacatct tctcttgaag
     5821 gaagccgggg atctgccaga tatatcttat gcatagtatc tttggtggct ataaaattaa
     5881 caagaaaatc tctggctaaa actttgttgg gagatttagc gttaatcata aaaccttgaa
     5941 cacctacaaa aggtttggct ggaagacctt cttcaaggtc aggaatcagc gctacaccat
     6001 agtctatacc tgcgtctttg taggctttca ccgcccatgg tccatttata atcattgctg
     6061 cctttccctc tttaaacaga gagtccataa tttcgtagtt atctccggtg gtcaaaattc
     6121 cttcgtctac taatcttttc cagagttctg ctccttttat tgctccggga ctggcaagac
     6181 cgatgtcagt tacatcaagt ccttcggggg tttctttgaa tacgtatcct ccagaaccga
     6241 gtatgaatgg agctgcataa taaaactcag ctgcgctggt tataaaccct cttacctctc
     6301 ctttataggt ggcatctatt cttttagcta tattgattaa ttcttccatg ttctttggag
     6361 gttcctcaac gtattccttg ttataaatta gagctatggc ttccatgctg tagggtagtc
     6421 cataaaggtt acccccgtag ctgaaggcat ccagggctgt agagtaaaag gtctcttgac
     6481 cttcaaaagg gggaacaggt tccagaagtc cgttgatcac cagttctccc acccaatcgt
     6541 gagctcccac gataatatct gctccttttc cttgaggagc agcggtcagg aaattgggct
     6601 ttatagaacc aaactctaca tactggacgt tcacactaac accatattcc gctttaaact
     6661 cttcacctaa tttctggagg atatctacct gtttttcaga gcaccagata gtaatctcgg
     6721 gagtttgggc tgcagaaata gaagaaaaca ccaaaatgaa caccaaaatc aaaggtaaaa
     6781 acttgaaact cttcattaat ataaccctcc ttttctgatg tttagtttac ggaatttaac
     6841 attccaaaaa catttttgac ccccttcgct gatctcttaa aaaccatttg atattgtttt
     6901 tattatatat ttccccctta tctttttcaa ctttcccttc taaacttgga cagtatgaac
     6961 ttattacaga ggttttaaga agaaatatta aaaaaagagg aaaaaagaga cctcattatc
     7021 cggtcttatt ggtgtatatt tgtaaaatac acaaatcact cataaatata acatagccaa
     7081 tatttgtagg attgagaaaa aatggaaatt actttccgta ccctagaagg agaacagatt
     7141 tcctggcttt ccagaaaaca ttacggaaag aattgaacca gctaggaaga gttatcctta
     7201 ttgacgtctt aaaactaaag tagcatttat taggatacaa agaagaaaaa agtttatgat
     7261 aaatatttct tttttaagaa gatcgtttat cagtttctct tgagttttga gagtttatag
     7321 ttaaccacaa aatccattgt aattaaccat catcacgagc cccgcctgat actaatggca
     7381 tactatgaga gcccttatga acccggtaat agggggaatg ctttccccta ttacccctga
     7441 aagagaaaaa aattaaaaac gtaaaacaag aacataaaga tgaaactacc gaggaagaaa
     7501 gagcatcttt ttcagcctcg cgatgacggg ttgaagaatt gctgtttttt tctgtcgctg
     7561 caacaactct cctcattcgt caccccggca atttttaagc cggggcctaa attatttaag
     7621 gaagaaaaca tctggattcc cgataaaagc gctcgagaat gacaaaagag agttataagg
     7681 ggaataaatc acccttataa ctctctgata aaaagcgaaa aaagacaatg aaaaatataa
     7741 aagacaaaaa ccaacttagg gagagaaaac atagtaccta atttagatgt aaaaactaaa
     7801 atataagaaa gcaagaaaaa taattaaaaa ctatgccaga aaagcacccg gcacagaggg
     7861 aagaaatgca tctctttttg cgctggacag tctatttttg ggttctcata agaagatatt
     7921 aggttaccac aggaaaagta aggagacctt cagaaagata atccggaaag gcagtaaaac
     7981 ccatacgata acccttgaag aatgattcct catcaagtaa agaacatcac aatgattttt
     8041 gtggttaact ataactgagg ctttttctcc attgataagt agtatatact attattcatg
     8101 agagaagaag gttctaatct ac
//
LOCUS       DAOPSS010000070         7794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_7772_length_7794_cov_8.464272, whole genome shotgun sequence.
ACCESSION   DAOPSS010000070 DAOPSS010000000
VERSION     DAOPSS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7772_length_7794_cov_8.464272"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /gene="rplM"
                     /locus_tag="PKN31_05310"
     CDS             <1..272
                     /gene="rplM"
                     /locus_tag="PKN31_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOA99376.1"
                     /translation="VVNAEKVKVTGKKKDQKLYRWHTGYPGGFKSETLGSLLNRRPEE
                     VIRRAVWGMIPHNRLGRKMIRKLKIYSGPSHPHQAQNPIPLKDKE"
     gene            278..673
                     /gene="rpsI"
                     /locus_tag="PKN31_05315"
     CDS             278..673
                     /gene="rpsI"
                     /locus_tag="PKN31_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOA99377.1"
                     /translation="MAVNPKYYATGRRKTAVAKVWLTLGSGKIVVNGRDFRDYFPLPV
                     WQILLQRPLALTGSEARFDVEAKVSGGGLSGQAGALAHGIARSLALVDENLRPTLKKA
                     GLLTRDPRMKERKKYGQRGARARFQFSKR"
     gene            772..1851
                     /locus_tag="PKN31_05320"
     CDS             772..1851
                     /locus_tag="PKN31_05320"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOA99378.1"
                     /translation="MAVSTYPLLEIDLSIIEKNIKVLLKKCASWHLHPVVVTKGFLAD
                     APLVYLFYRSGIRSFADSNLENLLHTKKLLPPEVRLSLIRLPMKEEVEEIVAQGIIPF
                     VSHWEMVEALSCEAEKQGKVLPLFLALESGDAREGFLPEEIEEMGDKIDDFPHIKVEG
                     ICSTLACLSGVLPDEEILERLVAFKKGWEEKWGRKIILSVGGTTFLSLWEKEKEIKGV
                     DEIRLGEALLFGNDISRGRNIEWLESGAFTLKAEIVEIKSKEPDRGKETGFDAFGESG
                     FSPEAGKRKRMILALGKQDSDEHQLYFADENAKIVGATSNYLVIDIEESNRVYQLGEV
                     VQFQAGYGAVLRAFLSPYVRKVYHW"
     gene            1862..2803
                     /gene="argF"
                     /locus_tag="PKN31_05325"
     CDS             1862..2803
                     /gene="argF"
                     /locus_tag="PKN31_05325"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOA99379.1"
                     /translation="MDRKRFRGRDFLDLVDYSREEIHYLLDTACELKRLDMMGIRDEK
                     PLKGKIVALLFEKPSLRTRVSFEVAVRHLGGESFYLGSQEVGLGQREAVKDVAQVLDR
                     MVDGLVVRTFAHENLLEIARFASIPVINGLSDLYHPCQVLGDLLTIQEKKGKLRGLKI
                     CFLGDGNNVCHSWINAASILGLELVVSTPERYRISSQIWEEAQERGKKNGARIIYEVD
                     PQKAIEGAEVVYTDVWASMGKEAEKEERKVFLQPYQVNSHLLSKASPDFIVMHCMPAK
                     RGEEITDEVMDGPHSVVVDEAENRLHAQKALLTLILS"
     gene            2816..3577
                     /gene="cdaA"
                     /locus_tag="PKN31_05330"
     CDS             2816..3577
                     /gene="cdaA"
                     /locus_tag="PKN31_05330"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOA99380.1"
                     /translation="MFPAWLWRFIPEVIILYYLFYRLGSLVRGTSLFLSFKWLPLFFL
                     LAGASNFLGFRELKFFWELLIFTYLAGSLIVFQPELRRVFFNRIHHRGESVRDYFFQD
                     EENRSEFINNIALACQTLSRKKIGALIVLERNDNLRDFIQTGVAIDAFFSPELLFSLF
                     LPESPLHDGAAILRENRIVAAGCILPLAESTEVKKLVGTRHRAGIGITEQTDALALVV
                     SEETGKISLAVHGKMAWDIEIGALKKMLKILYKKV"
     gene            3762..5846
                     /gene="fusA"
                     /locus_tag="PKN31_05335"
     CDS             3762..5846
                     /gene="fusA"
                     /locus_tag="PKN31_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOA99381.1"
                     /translation="MNSFTSKNIRNVGLFSHAGSGKTTLSEALLFNAGLLSRRGKVEE
                     GNTVSDWQPEEIKRGISIDLSILPWEWKGCKVNLIDTPGYADFVGNVLGAIRAVDSAV
                     VLLCATSGVEVGTEKVWEYISRENLPVIFFINRMDREGANFDEAVEELQEKINPRATP
                     LFIPVGKESDFRGVIDLLHFRALYFGKEGKVEEGEIPEEMKEVAQRARENLVENIAET
                     NDDLLDLYLEGQEISPSQLEEALRVAIRERHIFPILCGSGVENKGVDILADFLVDFAP
                     SPLERPPVRGINPLTEEEEERKCDENEPFSALVFKIISDPYVGKLALFRVFSGKISSD
                     SRLYNASRGVEEKVGQLLFLRGKNQESAKEIKAGDIGVVAKIDEVYIGDTLADREHPI
                     VFPPISYPEPTYMAAIRPLGKGDEEKISQALSRLMEEDPTFRLYRDPDTKEDIVYGMG
                     DIHLDVLTEKMKRKFGVEVSLTIPQVPYKETIKKPAKAEGKYKRQSGGRGQYGHAFLE
                     LEPLPRGQGFEFVDKIVGGVIPRNYIPAVEKGVQEAMQEGVLAGYPVIDVRATVYDGS
                     YHPVDSSDMAFKIAGSMAFKKGASEAEPVLLEPIMSVEVIVPEEYMGDVIGDLNSRRG
                     RILGMDPQDSYQKIRALVPLAELFRYSVDLRSLTQGQGYFTMKFSHYEEVPAQLAEAI
                     IEKAKKEKEAVS"
     gene            5843..6235
                     /gene="acpS"
                     /locus_tag="PKN31_05340"
     CDS             5843..6235
                     /gene="acpS"
                     /locus_tag="PKN31_05340"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOA99382.1"
                     /translation="MKQGILVGVDIVEIERVEKAVERFGDRFLDRLFLEEELNFYRSP
                     GRRREGIAALFCGKEAVKKLFLSKNFLLPWRQVKIEHTSGGKPEVKLSPLVPAFFEEI
                     DISISHSHQFAIAVAIGVEKEGVNNGTG"
     gene            6222..>7794
                     /locus_tag="PKN31_05345"
     CDS             6222..>7794
                     /locus_tag="PKN31_05345"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOA99383.1"
                     /translation="MELVSSQTMQKAEKALSEKYGVDTLLLMENAGRGVTEEIVKVLQ
                     CHSFSRVYIMCGMGNNGGDGLAIARHLRLRFPTLSILVYLVGKGKELKKDAQFNYQVV
                     KNLSIPVVEISSSQEVELERGALVVDALLGTGITREVEGIYREVIEKINASDNFVVAV
                     DVPSGVEASDGRVMGEAVRANLTVTLGLVKRGLCNFPGREYAGKIKLVDIGIPLSLEE
                     VKLKADGFLITPSMVKEMLPQRHWDTHKISAGVLGVVAGSSPLLGAGLLVCLGGYRSG
                     AGMVVWPLASSLSPLVKLFLPEVVSVNLSSSGEWHYSLSHWEVVEKAFQDRKCGALAV
                     GPGMGTNPSTRLFLEKVIENSRGVIDADALNIISQNIDSWKGHLTGWVITPHSGEMAR
                     ILGVSTPEVNENRIAASLEASSLLGCVTVLKGAGTVVASPRGEIFLNPTGTSVLASAG
                     SGDVLTGIIAGFMAQGMNSLDSAISGVFLHGLAGELLEREGKKQILSQEIAGKLNEAR
                     RMVENGEYTLSFLDGD"
BASE COUNT         2193 a         1377 c         2090 g         2134 t
ORIGIN      
        1 tagtggttaa tgctgaaaaa gtaaaagtga ccggcaaaaa aaaggaccag aagctatatc
       61 gctggcatac tggttatcct gggggattta aatcggaaac tttgggatct cttctgaatc
      121 gtcggccaga agaagttatt cgtcgagcag tgtggggcat gattcctcac aatcgtctgg
      181 gaaggaaaat gattcgcaag cttaaaattt attctggtcc ttctcatcct caccaggctc
      241 agaatcctat tccattgaaa gataaggagt gaataaagtg gcagtaaacc caaaatatta
      301 tgctactggt agaagaaaaa ccgcggtggc caaggtatgg cttactctgg ggagcgggaa
      361 aatagtggtg aacggccgcg attttcgaga ttattttcct ttgccagttt ggcaaatatt
      421 attgcaaaga cctctggctt taaccgggag tgaagctcgg tttgatgtgg aagctaaggt
      481 ttcaggagga ggattgagtg gtcaagcagg ggctctggct cacgggattg cccgttcttt
      541 ggccctggta gatgaaaatt tgcgaccaac tttgaaaaaa gccgggttac taacccgtga
      601 tcctcgaatg aaagagcgca agaaatacgg gcagagaggg gctcgagctc gcttccagtt
      661 ctccaagcgg taaagttttc tttttgcccc cgataggggg cttttcccca ttttcttgct
      721 cttaccgact cattgccttt tagaacatgg gggaagaaag gattgttcaa agtggccgtc
      781 agcacttatc cccttttgga aattgacctg agtataatag agaagaacat aaaggtcctt
      841 ctcaaaaagt gtgctagctg gcacctgcat ccggtagtag taactaaagg ttttttagct
      901 gatgcccccc ttgtttatct tttttatcgc tcagggataa ggagctttgc cgattctaat
      961 ctggagaact tgcttcacac caagaagcta ttaccccccg aggtgcgact ttcactcatt
     1021 cgccttccta tgaaagaaga agtagaagaa attgtagcac aggggataat accttttgtt
     1081 tctcactggg aaatggtgga agcactcagc tgtgaggcgg aaaagcaggg aaaggttttg
     1141 cccctttttt tagctttaga aagtggcgat gctcgggagg gttttcttcc tgaggagata
     1201 gaagaaatgg gggataaaat tgatgatttc ccccatatta aggtagaagg gatttgttct
     1261 actttagcct gtctcagtgg agttttgcct gacgaggaga tattagagag acttgtagct
     1321 tttaagaagg ggtgggaaga gaaatgggga agaaaaatta ttctctctgt gggagggact
     1381 acttttctct ctctgtggga gaaagagaag gaaataaaag gagtagacga aattcgcctg
     1441 ggagaagctc tactttttgg gaacgatatt agccggggaa ggaatataga gtggttagaa
     1501 agtggagctt tcactcttaa agcggaaata gtggagataa aaagtaaaga acccgataga
     1561 gggaaggaaa caggctttga cgcttttgga gaatctggtt tttcgccaga agcgggaaaa
     1621 agaaaaagaa tgattctggc tctgggaaaa caggatagtg atgaacatca attatatttt
     1681 gccgatgaaa atgctaaaat agtaggagcc accagcaatt acctggttat agatatagag
     1741 gaaagcaata gagtttatca attaggagaa gtggtgcaat tccaggcggg ttatggggca
     1801 gtgttacgtg cctttctctc tccctatgtg aggaaagtgt accactggta ggaggaaagc
     1861 tatggataga aaaagatttc gaggtagaga ctttttagat ctggttgatt attcccggga
     1921 ggaaattcac taccttttag acactgcttg tgagctgaaa aggttggata tgatggggat
     1981 aagggatgaa aaacccctga agggtaaaat agtggccctt ctttttgaaa agccctccct
     2041 gcgaacccgg gtttctttcg aagtggcagt gcgacacctg gggggagaaa gtttttacct
     2101 tggatctcaa gaagtgggat tgggacagcg agaggcagtg aaagatgtag cccaggtttt
     2161 agaccggatg gtggatggtc tcgtagttcg tacttttgcc catgaaaacc ttctggaaat
     2221 cgcccgcttt gcttctattc cagtgattaa cggtctttcc gatctttatc atccctgcca
     2281 ggtgttaggt gaccttttga ctattcagga gaaaaaagga aaactccggg gattaaaaat
     2341 ctgtttttta ggagatggca acaatgtctg tcactcctgg ataaatgctg cttctattct
     2401 ggggctggag ctcgtagtaa gcactccaga gcgctatcga atttcttctc aaatttggga
     2461 ggaagcacag gagagaggaa agaagaatgg tgctcgaata atttatgaag ttgaccccca
     2521 aaaagcaata gaaggagcgg aagtggttta tactgatgta tgggctagta tgggtaagga
     2581 ggcagaaaaa gaggagagga aagttttttt acaaccctat caggtgaata gtcatcttct
     2641 atctaaagct tcccctgact tcatagttat gcattgtatg cctgctaaac gaggagaaga
     2701 aattaccgac gaagttatgg atgggccaca ttcagtagta gtggacgaag cggaaaatcg
     2761 tcttcatgct cagaaagctc ttttgactct aattttgagt taggaggagg cctggttgtt
     2821 tccagcttgg ctgtggcgtt ttataccgga agtcataata ctatattatc ttttttatcg
     2881 tttgggtagc ctggtgcgag ggacttccct ctttttatct ttcaaatggc tgcccctttt
     2941 ttttcttctg gctggtgctt ccaatttttt aggatttcga gaactcaagt ttttctggga
     3001 actgttgatt tttacttacc tggcaggaag cttgatagtt tttcagcccg agctccggcg
     3061 ggttttcttt aaccggattc accatcgggg agagtcagtt agagattact ttttccagga
     3121 tgaggaaaac agaagtgaat tcatcaataa tattgcttta gcctgccaga ctctctcgcg
     3181 caaaaaaata ggagctttga tagttctgga acgtaatgat aatttgcgag attttatcca
     3241 gaccggtgtg gctatcgatg ctttcttttc tccggagctc ttgttctctt tgtttttacc
     3301 cgagtctcct cttcacgacg gtgctgctat cttaagggaa aatcgaattg tagcagcggg
     3361 gtgcattctc cctttagcgg aaagtaccga agttaaaaaa ttggtgggaa ctcgccaccg
     3421 agcaggaatc gggataaccg aacaaactga tgctctggct ttagtagttt cggaggaaac
     3481 tggtaaaata tctttagctg tacatggtaa aatggcctgg gatattgaga taggggcatt
     3541 gaaaaagatg ctgaaaattt tgtataaaaa ggtgtgaaaa gtggccagga tagaaagact
     3601 taaaaagagg tggcaagatt ttaaacggaa gcgaaaaaga gatagagaaa tcagaatttg
     3661 ggcttggtta ttagctttga gtttctgttt ttttctgtgg agcgaaatac tgtagtatcg
     3721 aaataaaaag acgaaggctt taaagaaagg gggattattc cttgaatagc tttaccagca
     3781 aaaacataag aaatgtaggt cttttttctc atgccgggtc gggcaaaact acactgagcg
     3841 aggctttact tttcaatgct gggctgttgt cccgtcgagg taaggtggag gaaggtaata
     3901 ctgtctctga ctggcaacca gaagagataa aaagaggtat atctattgac ctttctattc
     3961 ttccctggga atggaaggga tgtaaagtta atttaattga tactccagga tatgctgatt
     4021 ttgtgggcaa tgttttagga gcaataagag cagtggatag tgcagtggtg ttattgtgtg
     4081 cgacctcagg agtggaggtg gggacagaaa aagtttggga atatatttct cgggaaaatc
     4141 tgccggtcat tttttttatt aaccggatgg atagagaagg agccaatttt gatgaggcag
     4201 tagaggagtt gcaggagaaa ataaacccca gagccactcc tctttttatc ccggtgggca
     4261 aagagagtga ctttagagga gtgatagatt tacttcattt tcgagctctt tacttcggga
     4321 aagaaggaaa ggtagaagag ggagagatac cggaagagat gaaagaagta gcacaaagag
     4381 ctcgagaaaa tttagtagaa aacatagctg aaaccaacga tgaccttctg gacctgtatc
     4441 tcgaggggca agaaatctct ccttctcaat tggaagaagc tttacgcgtt gccatccggg
     4501 agcgtcatat attccccatt ctatgtggtt caggagtaga aaataaaggg gtggatatct
     4561 tagctgactt tttagtagat tttgctccct cacccttgga gcgccctccg gtgcggggaa
     4621 ttaatcctct caccgaagaa gaggaagaga gaaaatgcga tgaaaatgaa ccattttctg
     4681 cactggtatt caaaatcatc agcgaccctt atgtaggaaa actggctctt ttccgggttt
     4741 tttctggaaa aatttcttcc gactcccgtc tttacaatgc ctcacgaggg gtggaagaaa
     4801 aagtaggaca actcctcttt ttgcggggaa agaaccagga atcagcaaaa gagataaaag
     4861 caggagatat aggagtggtg gcgaaaattg atgaagttta cattggagac accctggccg
     4921 atcgggaaca ccccattgtt ttccctccca tttcctatcc cgaacctact tacatggcgg
     4981 ctatcaggcc tttgggtaaa ggagacgaag aaaaaatcag tcaggctctg tcccggctca
     5041 tggaagaaga tcctactttc cggttgtata gagacccgga taccaaggaa gacatcgttt
     5101 atggaatggg agatatccac ctggacgttt taacggaaaa gatgaagaga aaatttggag
     5161 tggaagtttc tcttactatt ccccaggttc cctataaaga aactatcaaa aaacctgcta
     5221 aagcagaagg aaagtataaa agacaatcgg gaggaagagg acagtatgga cacgctttcc
     5281 tggaactgga gcctctaccc cgggggcagg gctttgaatt tgtggataaa atcgtgggag
     5341 gggttatccc tcgaaactac attcctgcag tagaaaaagg ggtgcaggaa gcaatgcaag
     5401 aaggtgttct ggctggatat ccagtgatag atgtgcgggc tactgtttat gatggttctt
     5461 atcacccggt agattcttct gatatggctt ttaaaattgc tggctccatg gcgttcaaaa
     5521 aaggggcgag cgaagctgag ccggttcttt tagagccgat aatgagcgta gaggtgatag
     5581 tgcctgaaga gtatatggga gatgttatcg gtgacctcaa tagccgaaga ggaagaattt
     5641 taggcatgga tcctcaagat agctaccaga aaattagagc tctggtgcct ctggcagagc
     5701 ttttccgcta ctcagtggat ttacgctccc ttactcaggg gcagggatat tttactatga
     5761 agttttccca ttatgaagaa gtgccggctc aacttgctga agctataatt gagaaggcca
     5821 agaaggaaaa agaagcggtt tcgtgaagca gggaatttta gtgggagtgg atatagtaga
     5881 gatagagaga gtagaaaaag cggtagagag atttggtgat cgctttttgg acaggctttt
     5941 tctggaagag gaactcaatt tttaccgttc tccagggagg cggcgagaag ggatagcagc
     6001 tcttttttgt ggtaaagagg cggtaaaaaa actcttttta tctaaaaatt tccttcttcc
     6061 ctggcggcag gttaaaatag aacacacctc cgggggaaaa ccggaagtta aactttctcc
     6121 tttggttcct gctttttttg aagagattga catctctatt tctcacagtc accagttcgc
     6181 gatagcggtg gcgatagggg tagaaaaaga aggtgtaaat aatggaactg gttagctcgc
     6241 aaaccatgca aaaggcggag aaggcccttt ctgagaagta tggagtagat actttgctgt
     6301 tgatggaaaa tgctggacga ggagtgacgg aggaaatagt aaaagttctg caatgccact
     6361 ctttctccag agtttatata atgtgcggaa tgggcaacaa tggcggggat gggttagcga
     6421 tagctcgcca tcttcgcctc cgttttccta ctctttctat tttagtttac cttgtgggta
     6481 aagggaaaga gttaaaaaaa gatgctcagt ttaactacca ggtagtgaag aacttatcta
     6541 ttcctgtggt ggagatttct tcctctcagg aggtggagtt agagagagga gctctcgtgg
     6601 tagatgctct tttgggaacc ggtattaccc gggaagtgga gggaatatac agagaagtaa
     6661 tagaaaaaat aaacgcatcg gataactttg tggtggctgt agatgtcccc tcgggagtgg
     6721 aggcaagcga tggtcgggta atgggagagg ctgtgagagc caaccttacc gttactttag
     6781 ggttggtaaa aaggggatta tgcaactttc ccgggcggga atatgcggga aagataaaat
     6841 tagtggacat cggtatccct ctttccttag aagaggtgaa gctcaaagcc gatggatttt
     6901 tgattactcc atctatggta aaagaaatgc ttccacagag gcactgggat actcataaaa
     6961 tttctgccgg agtcttgggg gtggtagccg gctcttctcc ccttttagga gctggccttt
     7021 tagtatgttt ggggggatat cgctcgggag cggggatggt ggtgtggccc ttagcttctt
     7081 ctctttcgcc cttggttaaa ctctttcttc ctgaagtggt gagtgttaac ctttcttctt
     7141 ctggggagtg gcattattct ttatctcact gggaagtggt ggagaaagca ttccaggata
     7201 ggaagtgtgg agcgctggct gtaggtccgg ggatgggaac caatccctcc acacgtctct
     7261 ttttagagaa agtgatagag aactcccggg gagtgataga tgctgacgct ttgaatatta
     7321 tttctcagaa tatagacagc tggaagggtc accttaccgg ttgggtaatt actcctcatt
     7381 ctggagaaat ggcgagaatc ttaggtgttt cgacgccgga agttaatgaa aatagaattg
     7441 ccgccagctt agaagcttct tctcttctgg gatgcgttac ggttttgaag ggagcaggga
     7501 cggtcgtagc ttctcctcga ggagagatat ttttaaatcc tacgggaact tcagtccttg
     7561 ccagtgcagg aagcggtgat gttctgacag ggattatcgc cggatttatg gctcagggaa
     7621 tgaactcctt agatagtgca ataagcggag ttttcctcca tgggttggca ggagaactcc
     7681 tggagagaga aggaaaaaaa cagattttat ctcaggaaat agctggaaaa ctgaatgaag
     7741 ctcgaaggat ggtagaaaat ggagaatata ctctttcctt tctggatgga gatt
//
LOCUS       DAOPSS010000071         7773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_7792_length_7773_cov_8.276108, whole genome shotgun sequence.
ACCESSION   DAOPSS010000071 DAOPSS010000000
VERSION     DAOPSS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7792_length_7773_cov_8.276108"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKN31_05350"
     CDS             complement(<1..228)
                     /locus_tag="PKN31_05350"
                     /inference="COORDINATES: protein motif:HMM:NF040425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HOA99384.1"
                     /translation="MKKTNLESKISTLPLESRQVLIEHPALFELIRELIEIVEKQQQE
                     IAELQREVKHLRDQLHLDSHNSSYPPSTDKNR"
     gene            complement(312..1868)
                     /locus_tag="PKN31_05355"
     CDS             complement(312..1868)
                     /locus_tag="PKN31_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA99385.1"
                     /translation="MKKTGVLDRGKRLAHFYLFRYFRFYSRKFRPQDTIKREEIEKGL
                     QAVVKDGLASQAMSTLTGGAFLVDFALLLGASNFFIGVLAAIPALTQLIQIPATFLVE
                     KTRRRKLIVAIGAFISRIFLLLFALIPFLFSPSRRITALSLVILFHSACNSVVSCSWS
                     SWMRDFIPENILGSFFSRRMALSYSLGAVLSLAGAFYLDFWKRSFSDPLTGYSFLFLG
                     GVIAGFFSFYYLTLPPEPRLEGEEKAIGFAQLFLSPFKDQNFRNLLFFSGSWHFAYNL
                     ATPFFTVYLLRRLEYNMITVVILSTISQVMSILFFRWWGSIADHFSNKSVLLVSSPIF
                     LVSLFLWTFTTLPEKHAFTLPLLIIIHILLGISTAGILLATGNMGMKLAPRGQATPYL
                     ATYNLVNSLSLGIAPLLGGKLADFFAQRELSLSLNWISPTAERSFATLSFEHWDFLFF
                     FALLIGIYALHRLTFVEEVEEEKGTMGGLVTEIGRAVNNISSAGGIFLIENFRFPLRR
                     KEKKKKTYLY"
     assembly_gap    2193..2292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2792..3739
                     /locus_tag="PKN31_05360"
     CDS             2792..3739
                     /locus_tag="PKN31_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA99386.1"
                     /translation="MSILITGGNGLLGSWVTYYFAQKGKDVVSFDLKPRSFDYLEEYQ
                     DHVSFHRGDVLDWSSLVQVFQSQKIEGIIHTPAVMASPQYWDNPYPSTILNVVGTLNL
                     LEFARLYKVPKFLYVSSGAVYGETKDCPGELTHHINPSDLYGASKAGAEFLTLQYGNH
                     YGIDVRIVRPYFFFGPGYLPSEMTPVFRTLLGSIEGLDNLYLEKGRDQRLGFTYVKDT
                     AWGTVLAYEKENPSHRIFNIATDEATSFPDLAKLAQKYSDEPREVILGPGKLFPRGET
                     LDISLAKKELDFSPRYRMEEAVAEYARWIKKAKSRKECG"
     gene            complement(3756..4412)
                     /locus_tag="PKN31_05365"
     CDS             complement(3756..4412)
                     /locus_tag="PKN31_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOA99387.1"
                     /translation="MDSLLFIHNELFPELWKGWMVTLKLVALSIPFGLLTGILIAIGR
                     VYGNRLISSLCRLYVIFFKGCPLLLLLFVIYFGLPSLGIAFSPFVASVLGFVLCNGAY
                     TSEYIRGAIQSVKTGQMIAAQALGMTKWQAVFYIILPQAFRRALPGVGNEMIYLIKYS
                     SLAYMLTCVELTGAGKIVAARSFRFMETFAVVGIIYLFMVSIATKILQWAEKKLSYPG
                     "
     gene            complement(4405..5172)
                     /locus_tag="PKN31_05370"
     CDS             complement(4405..5172)
                     /locus_tag="PKN31_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOA99388.1"
                     /translation="MSTTPVLVVEDIHKSFGKVEILKGASFAVNKGETKVIIGPSGAG
                     KSTLLKCINQLVIPDRGRVFLNGEEVLHSHSQINRFRQKIGMVFQNFYLFDHITALEN
                     VEIALLKVKKMKRAEAREKALEELNRVGMGDKVHLYPAELSGGQAQRVSIARALAMDP
                     EVILFDEPTSALDPELTGEVLEVMRDLAKEGMTMVVVTHEMGFASSVADEVLFMEEGR
                     FLESGPPSQIFFHPREERTKKFLSRISELYGELNKNG"
     gene            complement(5169..5849)
                     /locus_tag="PKN31_05375"
     CDS             complement(5169..5849)
                     /locus_tag="PKN31_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOA99389.1"
                     /translation="MEGLSAVLESLPYILSGIPVTLGLVALSLALGFTLGVPMAVATV
                     YGNRVMAKIIQVFVFVMRGLPNLVLLFLFYFGIFPLLGWNLSPFVIGIITLGLRSAAY
                     QTEIFRGAILSLEEGQMLAALSVGMTKNQAIWNIILPQALRLALPGWSNEYPILLTDS
                     SVTYAIGVAEILTRGSQMVTRTYQPMPIYLTCALIFILLNWGGMQIIQWGEKKAYIPG
                     LGRKIETR"
     gene            complement(5861..6619)
                     /locus_tag="PKN31_05380"
     CDS             complement(5861..6619)
                     /locus_tag="PKN31_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99390.1"
                     /translation="MKKYGVILLALLVLMLGVVNGGWAEEKKTYIVGIDGDYYPFSYI
                     DKDGSPAGFDVESIQWIAQEMGFEVKIVPIAWDGIVPSLLAKKIDMIYSGMTVTEERK
                     EKVDFTDVYWVVNQAVATKEEASWTLEDFLAGKCSIGTQRGCSAAMWIEDNLVKPGIL
                     PENKLKLYDNFPLAANDLLNGRVETIIMDEPIIDKAIEGKPLKKIGVISTGEEYAIAV
                     RKEDTELKALLNEGLKRLMSSPYWEELIEKYEMK"
     gene            complement(6715..7635)
                     /locus_tag="PKN31_05385"
     CDS             complement(6715..7635)
                     /locus_tag="PKN31_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99391.1"
                     /translation="MNSFQYNLARFISFQDKAECERVRQIKKEDITNHPNPDFKIQVI
                     EERDDFYFQFAMDIFTRIVEAREKKESLVLILPVGPVPQYSLVAKMVNQFHIPLDHLH
                     IFNMDEYADEEGNTAPPSWPGSFQKAMWDNFFSLIDPPLRPLEKNIHFPTKEVLPDYA
                     KMIEDLGGADCCYGGVGWCGHIAFWEAHLGFEFGDDLQAFQEAGPRLVELHPMTIMQN
                     ALHSFSGDWSWVPPFANTIGPAQIVGAKYRSFWLDGYLGGGVSWQRFIARLAAHGRVN
                     TLVPASILQNFPGTYTLLGGVADNVEIKMA"
BASE COUNT         2285 a         1640 c         1563 g         2185 t
ORIGIN      
        1 tctattttta tctgtagatg gaggataact gctgttgtga ctatccagat gaagttgatc
       61 tcgaaggtgt ttcacttctc gttgaagttc ggctatctct tgttgctgtt tttcaacaat
      121 ctctatcaat tccctaatga gttcaaataa agcagggtgt tctatcagaa cctgtcgaga
      181 ttctaaaggt agggttgaga ttttactttc cagattagtt tttttcatag tgcacatagt
      241 attcatgact cttaaagaac tgtcaacaga acctcaataa aaatataggc ccctatgtag
      301 ttacttgttt tttaataaag gtatgttttt ttcttttttt cttttcttcg gagaggaaaa
      361 cgaaagtttt ctattaggaa aattcctcca gctgaagaaa tgttattaac tgctcttcct
      421 atctcggtta ccaaccctcc catggttcct ttttcctctt ctacctcttc aacaaaagtc
      481 aaacgatgaa gagcgtatat cccgattaag agagcaaaaa agaacaggaa atcccagtgc
      541 tcaaaagaga gagttgcaaa agagcgctca gctgtggggc ttatccagtt gagagaaagg
      601 gacagctctc gctgagcaaa aaagtcagcc aattttcctc ctaaaagagg agcgataccc
      661 agtgatagag agttaaccag gttataagtg gctagatacg gagtggcctg tccccgggga
      721 gctaatttca tacccatgtt cccggtagct aagagaatgc cagcagtaga aatccccagt
      781 aaaatgtgaa tgatgatgag caacggcaga gtgaaagcgt gtttttccgg taaagtggtg
      841 aaagtccaca agaatagaga aaccagaaat atagggctac tgactaaaag aacagattta
      901 ttggagaaat ggtcagcaat gctgccccac caccggaaaa acaagatgct cataacctgg
      961 cttatggtgg ataagataac aactgtaatc atattgtatt ctaaacgccg taagagataa
     1021 acagtgaaaa aaggagtggc taagttataa gcaaaatgcc aggaaccaga aaagaagagt
     1081 aaattgcgaa aattctggtc tttaaaagga gaaagaaata gctgagcaaa accaatcgct
     1141 ttttcctctc cttccaggcg aggttcggga ggaagggtta aatagtagaa actgaaaaaa
     1201 ccagctatca ctcctcctaa aaaaagaaag gaatagccag tgagagggtc agaaaaagag
     1261 cgtttccaaa aatccagata aaaagctcct gcgaggctca gcaccgctcc taaggagtaa
     1321 gaaagagcca tacgacggga aaagaaactc cctaagatat tttcagggat aaaatctcgc
     1381 atccaggaag accagctgca agagaccaca gaattacagg ctgagtgaaa aagaatgaca
     1441 agagatagag ccgtaatcct ccgggaagga gaaaataaaa aagggatgag agcaaagagc
     1501 aaaagaaata tacgacttat aaacgcccct attgctacaa tcagcttcct tctgcgggtt
     1561 ttttctacta aaaaagtagc aggaatctgt atcagctggg taagggcggg aatagcagct
     1621 aaaactccga tgaagaaatt ggaagccccg agaagcaggg caaagtctac cagaaaagct
     1681 cccccggtca aggtagacat agcctgactt gctaagccat cttttactac tgcctgcagc
     1741 cctttttcaa tttcctctct cttaatggtg tcctgggggc gaaattttcg ggaatagaaa
     1801 cggaagtaac ggaataaata gaaatgagct aatctttttc ctcggtctaa cactccagtt
     1861 ttcttcaatc tttttacctc tcagagagaa attcctgttt aatattacgg tctttcttaa
     1921 gagatagttc ctctattatt atagagggga aatatagttg accgcaaaac ctgttaagct
     1981 gttacaactt taaaaagcaa attacattgg aaattacaaa ctcgagatcg ccacacacaa
     2041 aagaaggctt ttctttaatc tcgcgccgta ggctgtctga ataacaaaac cccggctaaa
     2101 gatatgccgg ggtagtaaat aatctaaact cacgacttct tattagcttt taaaggcagg
     2161 cgaagctcct tttttttatt tcctaactgg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nntcttatta gcttttaaag gcaggcgaag ctcctttttt ttatttccta
     2341 actggtcacg tacgattatt tctaaaacat agtctcctgg tccaaggtca aagttgaact
     2401 tgaaacaaaa gaagatttca taaaccggtt ttttggtgat atagcgcata ttttcatctg
     2461 tgatggtata acttcaagac ccagcataca tacagatgga atcaccagga gaaagaaggt
     2521 agagagtttt ccttatccac cccaaaacta tatagttttc cttccacaaa aaggtactct
     2581 gctatcttaa gaggggcttt attccataag ataaatgtgc ttccttcagg aatattgggc
     2641 ttcctcgatg cttcctcctt tgcaaggtta agagcttttt ccagtaaatt tttctcctct
     2701 tattttttca tcttttgaac ccctttcttt tagattttct gatatcatag aagaaaagta
     2761 aaaatagaaa taaaccggag gaagtgataa tatgtctatt cttataaccg gtggcaatgg
     2821 tcttttaggt tcctgggtaa cctactattt cgcccaaaag ggaaaagatg tagtctcttt
     2881 tgatttaaaa cctcgttctt ttgactattt ggaagaatac caggatcatg tttccttcca
     2941 cagaggagat gttctggact ggtcaagcct ggtacaggtt tttcagtctc agaaaataga
     3001 gggtataatt cataccccgg cggtgatggc ttccccccag tactgggata acccttatcc
     3061 cagtactata ttgaatgtgg taggaactct caatctatta gagtttgctc gactttataa
     3121 ggtgccaaag tttctctatg tgagttccgg agccgtttat ggagagacta aagattgccc
     3181 gggagaactc acccatcata tcaacccttc tgacctttat ggagccagca aagctggagc
     3241 agagtttctc actctccagt atggcaatca ctatggaata gacgttagaa tagttcgtcc
     3301 ttactttttc ttcggtcctg ggtatctccc ctctgagatg accccggttt tccgcactct
     3361 tttaggttct atcgaaggac tagataatct ttatctcgaa aaaggccggg accagagact
     3421 gggatttacc tatgtgaaag atactgcctg gggaacagtg ttagcttatg aaaaagaaaa
     3481 tccctctcat cgaatattta atatcgctac tgatgaggca actagttttc ctgatttagc
     3541 taaattggct caaaaatata gtgatgagcc cagagaggta atattaggtc cggggaaatt
     3601 gtttcctcga ggagaaactc tggatatttc tttagctaaa aaggagttgg acttttctcc
     3661 tcgttatcga atggaagagg cagtggctga atatgctcgc tggattaaaa aagctaaatc
     3721 ccggaaagag tgtggatgaa tacatacata cttctttagc caggatagga gagtttcttt
     3781 tctgcccact ggagaatttt agtggctatg gagaccataa atagataaat tatacccacc
     3841 acagcgaaag tttccataaa acggaaactt cgagcagcca cgattttccc tgctccggtt
     3901 agctctacac aggtgagcat ataagctaaa gaagagtatt tgatgaggta aatcatttcg
     3961 ttgcccacgc caggtagtgc ccggcgaaaa gcttgaggaa gaatgatgta aaataccgcc
     4021 tgccacttgg tcattcccaa agcttgcgca gcaatcatct ggccggtttt taccgattgg
     4081 attgctcctc ttatgtactc agaagtatag gctccgttac agaggacaaa ccctaaaacc
     4141 gaagccacaa aaggtgaaaa agcaattcct aaggaaggga gtccgaagta aattacaaat
     4201 aagagaagaa gaagaggaca ccctttaaaa aagatgacat aaagccgaca gagggaggaa
     4261 attaagcgat ttccgtatac tcttcctatg gcaatcaata tcccagtcaa cagtccaaaa
     4321 gggatagaaa gagcgactaa ttttaaagtg accatccatc ctttccatag ctcggggaac
     4381 aattcattat ggataaaaag caagctatcc atttttgttt aactctccat atagctcaga
     4441 aatccggctg aggaattttt tggttcgttc ttcacgggga tggaaaaaaa tctgggaagg
     4501 aggtccactt tctaaaaatc tcccttcctc cataaataga acttcatcag caactgagga
     4561 ggcaaaaccc atctcatggg taaccaccac catggtcatc ccctctttag ctaagtctct
     4621 catgacttcc agcacctctc cggttaattc cgggtctaaa gcagaggtag gctcatcgaa
     4681 taatattacc tcaggatcca tggctaaagc tcgggcaatg gagactctct gtgcctgtcc
     4741 tcctgatagc tcagcaggat aaagatgaac tttatccccc attcctaccc ggtttagttc
     4801 ctccagagct ttttctcgtg cctcagcgcg tttcattttt ttgactttaa gaagagcaat
     4861 ttccacattt tctaaagcgg tgatatggtc gaaaaggtaa aaattctgga agaccatccc
     4921 tattttctga cggaaacgat taatttgaga atgagaatgt agcacttctt ctccattgag
     4981 aaatactctt cctctatcag gaatgacgag ctgattgatg catttaagga gggtactttt
     5041 gcccgcgcct gaagggccga tgataacttt ggtttctcct ttatttacag caaaagaggc
     5101 tcctttgaga atttccactt ttccaaaaga tttatggatg tcttccacta ctaaaacagg
     5161 tgtagtactc atcgagtttc tattttcctt cctaatccag ggatataagc ttttttttct
     5221 ccccactgga tgatttgcat tcctccccag ttgaggagga tgaagattag agcacaggtt
     5281 agataaatag gcataggctg ataggttctg gtaaccatct ggctaccccg ggttaaaatt
     5341 tcagctactc caatagcata agttaccgaa gaatcggtaa ggagaatggg atactcgtta
     5401 gaccacccgg ggagggccag gcgtagagct tgaggtaaaa taatgttcca gatggcttga
     5461 ttcttggtca tgcccacaga tagagcagct aacatctgcc cctcttccag agagagaatg
     5521 gctcctcgga aaatttccgt ctgatacgca gcacttcgca agcccagagt tataattccg
     5581 attacaaagg gcgatagatt ccaccctaaa aggggaaaga tgccaaaata aaaaagaaag
     5641 agaagcacta aattgggcag gcctctcatc acaaaaacaa atacctggat tatttttgcc
     5701 attacccgat ttccatagac cgtggctacg gccattggta ctcctaaggt aaaccccaga
     5761 gccagagaca gagccacaag tcctaaagtt accggtattc cagataaaat gtaaggtaga
     5821 gattctaaaa cagcagagag gccctccact tttctccttt ttacttcatc tcgtattttt
     5881 caatcagttc ttcccagtag ggggaactca tcaatctttt caatccttcg ttaagcagag
     5941 ctttaagctc agtatcttct tttctcactg cgatagcgta ttcttctcct gtggaaataa
     6001 ctccaatttt cttcaatggt tttccctcga tagctttatc tattatcggt tcatccatga
     6061 ttattgtttc tacccggccg ttgaggaggt cgttcgctgc taaggggaag ttgtcataga
     6121 gtttaagttt gttttcgggg aggattcccg gttttactaa attatcttct atccacatag
     6181 ctgctgaaca gcctctttgc gtacctatac tgcattttcc agccagaaaa tcctcgagag
     6241 tccagctggc ttcttctttg gtggccactg cctggttcac cacccaatac acatcggtaa
     6301 aatctacctt ctctttgcgc tcttcagtaa ctgtcatgcc tgagtatatc atatcgattt
     6361 ttttagcgag caggctgggg actatcccat cccaggctat aggaacaatc tttacttcaa
     6421 accccatctc ttgggcaatc cattgaatgg actctacatc aaaccctgcc ggacttccat
     6481 ctttgtcgat gtaactaaaa ggatagtaat caccgtcaat tcccactatg taagtttttt
     6541 tctcctctgc ccaccctcca ttaactactc ctaacattaa aacgagtaga gccaatagaa
     6601 ttactccgta ctttttcatt ttcatcctcc cttaaagatt tattctttct aatattaaat
     6661 aatggattat ttactgtcaa ttggataagc aaactctccg cagagaaaag agttttaagc
     6721 cattttaatc tctacattat ctgctactcc ccccaatagc gtataagttc cagggaaatt
     6781 ctgcaagata gaagcaggaa ctaaagtgtt cactcgtcca tgtgctgcca aacgagcaat
     6841 aaatctttgc cagcttactc caccccctaa gtatccgtct aaccagaagc ttcggtattt
     6901 agctcccaca atttgagctg gtccgatagt gttggcaaat ggaggaaccc atgaccaatc
     6961 tccactgaaa gaatgtagag cattttgcat aatggtcata ggatgaagtt ctactaatcg
     7021 cggccctgct tcttggaaag cctggaggtc atcgccaaac tcaaatccca gatgagcttc
     7081 ccagaaggcg atatgcccgc accagcccac tcctccgtag cagcaatctg cccctcctaa
     7141 atcttcaatc attttggcat aatcgggaag aacttctttg gtgggaaaat gaatgttttt
     7201 ctccagaggg cgaagagggg ggtcaatcaa gctgaaaaag ttgtcccaca tagctttctg
     7261 gaaggaacca ggccatgatg gaggagcggt atttccttct tcgtcggcgt attcgtccat
     7321 attgaaaatg tggagatggt ctaagggaat gtgaaattga ttgaccattt ttgctaccaa
     7381 tgagtactga gggactgggc ctacgggaag gataagaacc agactttctt ttttctctcg
     7441 agcttccact attcgagtga agatatccat agcaaattga aagtagaaat cgtctctttc
     7501 ttcaatgacc tgaattttga aatcaggatt gggatggttg gtaatatctt cttttttaat
     7561 ctgacgtacc ctctcacact ctgccttatc ttgaaaagat ataaatcggg ccagattata
     7621 ctgaaaagaa ttcattttaa aacctccttt tttcttttag tataccccaa aagggataat
     7681 tcttacatct cttattaact ctctttcctc tgcgaggtat ggagaacttc cctctttctg
     7741 tgtatgttta tagagtttta ccgatccgga gag
//
LOCUS       DAOPSS010000072         7751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_7827_length_7751_cov_8.404106, whole genome shotgun sequence.
ACCESSION   DAOPSS010000072 DAOPSS010000000
VERSION     DAOPSS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7827_length_7751_cov_8.404106"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PKN31_05390"
     CDS             complement(<1..757)
                     /locus_tag="PKN31_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99392.1"
                     /translation="MKKAVLACFLVLVLVLGGVAFGQQVQIPREEALYVAGFQWGPPT
                     TDNPFTGSPMTFVSNPRQHIWIYEPLFTYDGLLTGDFVPILGESFQWRDDLTLEVKLN
                     PNAYFHDGEKVTADDVVYSYKLGQKYPIGYSSVWDWLEDVYKEDDQTVIFKMKADNPN
                     KLMVEDGLGAAYIIPEHIWAKVEEDAGNDIAEIRKFDNKDPIGSGPYRVYYESPETII
                     LERVDDYWGVVLYDGELPRPKYVVHPIFKSNDEG"
     gene            complement(857..1852)
                     /locus_tag="PKN31_05395"
     CDS             complement(857..1852)
                     /locus_tag="PKN31_05395"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOA99393.1"
                     /translation="MERILPATDENIKKGAKILKEGGLVAFPTETVYGLGANVFLPEA
                     VARIFEVKGRPRFDPLIVHISRHGMVSRLFREIPPLAEMLMEKFWPGPLTLVLPKKEE
                     VPDIVTAGLSTVAVRMPAHPVALKLIDWADVPVAAPSANRFGHLSPTQAEEVALDLGE
                     GVDLILDGGKTEIGVESTIIMIEEGKVFLLRPGGLSIEEIEKWVGKIETPPSSPKVLS
                     PGTLKKHYAPRIPLYLFEGKIEDLLLCDLSGIAIVSPFPIADSREEIKVLSEKGDIRE
                     VASNLFSVLRELEEGPFRAIISLTVEEKGLGRAIMDRLKRASSGYVKVQDRKLIV"
     gene            1932..2687
                     /locus_tag="PKN31_05400"
     CDS             1932..2687
                     /locus_tag="PKN31_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOA99394.1"
                     /translation="MTGQSIILGLIQGLTEFFPISSSAHLILLRPIFNLGEQSLWFDV
                     LLHGGTWLAVLIYLIPYYSRAFRQPRIILNILLATIPAGLLGYLFEDIIENSLRNSFL
                     EICLLLVILGVIFLVVKERGNKTLENMTGKEALLIGVAQALALFPGVSRSGITLLAGF
                     LCGLQKKEAVLFSFFLSATTIGGAFILGIRNLSVSGNFLSPELVSGFLASFGSGLLAC
                     FLLLRIVKEKGLKPFGYYRIIFGGTMFIWFLFH"
     gene            complement(2684..2986)
                     /locus_tag="PKN31_05405"
     CDS             complement(2684..2986)
                     /locus_tag="PKN31_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99395.1"
                     /translation="MIKLILEPVPYRYEETVYFYWEYELNSPYQLCFIPFRSLNKFLE
                     EMVRRETEIPESIHIILEDGRIKIWLPVAFYSEQLFNKVENILREKIRSLLEDVMF"
     gene            complement(2987..3622)
                     /locus_tag="PKN31_05410"
     CDS             complement(2987..3622)
                     /locus_tag="PKN31_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOA99396.1"
                     /translation="MNESWELLSIFGSAFLIGFSGALMPGPLLALVLSGTYQVGYKAG
                     PLVVLGHGILELGLVIALMLGLGKVLDIPLVHQVIGIGGGVVLLYLGIDMLRMVRNSE
                     IFSVENSSNSEGGLAFKGLLVSLSNPYWIMWWATVGLALLLSATSFPFWGVVFFFLGH
                     ILSDFVWYSAVSFTLHRGKNFLSPRFYQWLVGICGGFMLFFGIYFISEVIR"
     gene            4235..5971
                     /locus_tag="PKN31_05415"
     CDS             4235..5971
                     /locus_tag="PKN31_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99397.1"
                     /translation="MKKLLSYLKPYWKAALLAPILMLIEVICDLSQPTLLASIVDNGV
                     AKSNLPYILNTGVLMLGIALIGMAGGMGCSVASSIASQNFGASLRSAVFKKIQLLSPL
                     NIDRFKTSSLVTRLTNDITQLQTVVLMSLRIMVRAPLLFIGGIIMAVSLNARLALILT
                     VSIPLLAAVIYYRMKKSTPLFSYMQEKLDRVNAVIRENLSGIRLIKAFVRADHEKKRF
                     ATANDELMYATLRAFNLVITMMPIVMLIMNFSIVAVLWFGGWDIDRGNMEVGEIIAFV
                     NYITQILFSLMMIGNILLFVSRASASAERVNEVLDTDIDIKNADNPDFTPLSSGEVAF
                     ENVSFGYSGDKNQLVLKEISFHAPSGKTIAILGSTGSGKSTLVNLIPRFYDVTSGQVT
                     VDKRDVRSMDLATLRSGMGIVPQDTILFSGTIKDNIRWGKEDATDEEIIEAARVAQAH
                     EFIMSFPEGYDTQLGQRGVNLSGGQKQRIAIARAIIKKPPILILDDSTSAVDVVTEQR
                     IQKALKEFIRGSTIFIIAQRISSVMDADKILVLNEGRIEAEGTHEELLKSSPLYQDIY
                     NSQLGEGVMIDA"
     gene            5964..>7751
                     /locus_tag="PKN31_05420"
     CDS             5964..>7751
                     /locus_tag="PKN31_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99398.1"
                     /translation="MHNPGGGRHDAVYVVKPRDAKNTLKRLWWYLSEHKIRLFLVLFL
                     VAITSVLTLVGPYLIGKAIDNYIIPRDFNGLFRLLILMAAIYVLMSLFTWLQSRTMIN
                     VAQLTIRNMRKDAFDKLQILPVSFFDARPRGDIMSRLTNDIDLINNALSSSLTQIFSS
                     IITLIGTVILMLWLSPLLTGVSMITVPIMLITTNIVTRHTRRYFSEQQRVLGMLNGFA
                     EENISGQKVVKAFVREAKEIERFEVTNQELKNVGIKAQIYSGIMGPLMNVLNNIGFAI
                     VALSGGWLAVKQIITIGTIAAFINYTRQFTRPLNELANQINTIQSAIASAERLFEIMD
                     EPPEPPDPPDAIELKSVKGDVEFRNVSFSYKSDEAVLKNISFHAHPGQTIALVGPTGA
                     GKTSIINLLARFYDPDSGDIFIDGYNIQKITRKSLRSSLGIVLQDTYLFSESVKDNIR
                     YGRLDATDEEVKAAARLANAEQFILNLPQGYDTILSEDGGDLSQGQRQLLAIARAILA
                     DPAILILDEATSSVDTRTEQHIQEAMYKLMKGRTSFVIAHRLSTIRKADMILVIDDGE
                     IIERGNHVQLLKQKGFYYNLYMSQFAHILH"
BASE COUNT         2419 a         1697 c         1503 g         2132 t
ORIGIN      
        1 ttccttcgtc attactctta aaaatagggt gaacgacata ttttggtctg ggcagttcgc
       61 catcgtagag aaccactccc cagtagtcat caactctttc cagaattata gtttctggac
      121 tttcataata aacccgataa ggtcctgagc ctatggggtc tttgttgtcg aatttcctaa
      181 tttctgctat gtcattgcca gcatcttctt ccacttttgc ccagatatgt tcaggaatta
      241 tgtatgcagc acccaaacca tcttccacca ttaatttatt gggattatca gctttcattt
      301 tgaaaataac agtctggtca tcttctttgt aaacatcttc cagccaatcc cagacggaag
      361 aatagcctat tggatatttc tgaccaagct tataggaata aactacatcg tcagcggtga
      421 ctttttctcc atcatgaaaa taagcattgg ggttcaactt tacttccaga gtcaaatcat
      481 ctcgccactg gaacgattct cccagtatgg gaacaaaatc accggttaag agaccatcat
      541 aggtaaacaa gggctcgtaa atccagatat gttgccgggg gttagaaaca aaagtcatgg
      601 gactcccggt aaaaggatta tcggtggtag gaggacccca ctgaaatcct gcaacataga
      661 gagcttcttc tcgaggaatt tgcacctgtt gaccaaaagc taccccccct aaaaccaaaa
      721 ccaacaccaa gaaacacgct aaaactgcct ttttcatatt tatcacccct ctttcatatt
      781 agtcatagag tgtactctat gactaatatg aattatagca taagtaatta tcaattagga
      841 ataaatacca tgaggattac acgattaatt ttctatcttg cactttaaca tatcccgaag
      901 acgctctttt caaccggtcc ataattgctc ttcccaatcc tttttcctct acggttaggc
      961 tgattatggc ccgaaaaggg ccttcttcca actctcgcaa aacactgaag agattactgg
     1021 ctacctctct tatatcccct ttctcactca aaacctttat ctcttctcgg gaatcagcaa
     1081 tcggaaaagg agaaacaata gctattcccg aaagatcaca caagaggaga tcctctatct
     1141 tcccttcaaa aaggtaaaga ggaatccggg gagcataatg cttctttaaa gtaccaggag
     1201 aaagaacttt aggggaagaa ggcggtgtct cgatttttcc tacccacttt tcgatttctt
     1261 caatactcaa ccctcctggc cgaaggagaa agacctttcc ctcctctatc atgattattg
     1321 tagattccac cccaatctct gtttttcctc catccagaat caaatccact ccttctccta
     1381 agtcaagagc cacttcctct gcttgagtag gactcagatg tccaaaacga ttggcactgg
     1441 gagcagccac agggacatct gcccaatcta tcaactttaa agctacagga tgagctggca
     1501 tgcggacagc cacagtggaa agaccggcag taacgatatc aggaacttcc tcttttttgg
     1561 gtaaaaccag agttaaagga ccaggccaga acttttccat caacatctcc gcaagaggag
     1621 gaatttccct aaagagacgg gaaaccatcc catggcgaga aatatgcaca ataagaggat
     1681 cgaaacgagg tcttccttta acttcgaaaa tacgagccac cgcctcaggt aaaaacacat
     1741 tagcccccag tccatagacg gtttcagtgg ggaaagctac taaccctccc tcctttaaaa
     1801 tttttgctcc ttttttaatg ttttcatctg tagccggaag aattcgctcc atttttctcc
     1861 ttttctcctg atattgctct ctttttagtt ctattataga atagcaagca aaaccgagga
     1921 aggagtcagc tatgaccggg caatcaatta tattaggttt aattcaggga ttaaccgagt
     1981 tttttcccat ttcttcttct gctcatctta ttcttcttcg cccgatattc aatctgggag
     2041 aacaaagcct gtggtttgat gtgctgctcc atggaggaac ctggcttgca gtattaattt
     2101 accttatacc ttattattcc cgggcttttc gtcagccccg cattattttg aatatcctcc
     2161 tggccactat ccctgcagga ttactgggat atttatttga agatataata gaaaattcct
     2221 tgagaaacag ctttttagag atatgtttat tattagttat tttaggtgtg atatttttag
     2281 tggtaaaaga aagagggaat aaaactttag agaatatgac tggaaaggaa gctctcttaa
     2341 tcggggtagc tcaagctctt gctcttttcc cgggggtttc ccgctcaggt attacccttc
     2401 tggctggatt tctctgcggg ctacaaaaaa aagaagcggt gcttttttcc ttctttttat
     2461 cagctaccac cattggagga gcatttatct taggaattag aaatttgagt gtttcgggaa
     2521 acttcctctc accggagctg gtgagtggat tcctggcttc ctttggtagc ggattgctgg
     2581 cttgctttct cctactgagg attgtgaaag aaaaagggct gaaacctttt ggttactatc
     2641 gcatcatttt tggggggaca atgttcatct ggtttttatt tcattaaaac ataacatctt
     2701 cgagaagaga gcggattttc tctctcaaaa tattttccac tttgttaaat aattgttcag
     2761 aataaaaggc aaccggcaac catattttta ttcttccgtc ctctaaaatg atatggatac
     2821 tttcaggaat ttcagtttcc ctcctcacca tttcctctaa aaacttatta agactcctaa
     2881 agggtataaa acagagctga taagggctat ttagttcata ctcccagtaa aagtaaacag
     2941 tctcttcata tcggtaaggt accggctcta aaattaactt aatcatttat cgaatcacct
     3001 ctgagataaa ataaatacca aagaaaagca taaaccctcc gcaaatcccc actaaccact
     3061 gataaaaacg gggagagagg aagtttttac ctcgatgcaa agtaaaagaa accgcgctat
     3121 accagacaaa gtcagagaga atatggccaa gaaagaaaaa caccactccc caaaagggaa
     3181 aagaagtagc agaaagaaga agagccaacc ctacagtagc ccaccacatt atccagtaag
     3241 gattagaaag actgaccagt aaacctttaa aagccaggcc accttctgag tttgagctat
     3301 tctctacaga gaaaatttct gaattacgca ccatcctcaa catatctatt cccagataca
     3361 ataaaactac ccctcctcct attcctataa cctggtgaac taaaggtatg tccagcactt
     3421 tccccagacc aagcattaaa gctataacca aacccagttc taaaattcca tgacccaaaa
     3481 ctaccagggg tccagcttta tagcccacct ggtaagtccc ggaaagaacc agagccaaaa
     3541 gcggaccggg catcaatgct cctgaaaaac caatcaggaa agcactgcca aagatagata
     3601 ataattccca gctttcgttc atttcctttt ttttatttat tatataacaa tcagcgagat
     3661 atttactgga aaaaacttaa aaataaaatc tactgaggga gaaaaaaccc ctcaacaaaa
     3721 aacttctgga ttcccgattg aagatttcgg taatgacgta agaagatgaa cgcctcgcta
     3781 aatttcaggc ataatagacc tttccttttc tcaccttgaa atcctctttt ttattcgcct
     3841 cctgcacttt ctccactgac tcttttcttt ctcccttccg caagagatga gaaaaggaga
     3901 agcccgcaag gggtgggaga aagaaaaaaa tcccacgagg atcgcaaaaa cagcctacgg
     3961 cgccaaaagc aaaagagcaa aaaattgaaa ccaaaaacta aaacaattac actggataat
     4021 acgccagaat catgattaca aaatggagag tgaattgcct taaaaaagtg gcctgactcc
     4081 cttttacaaa aatttagagc acttatccac ttactgcaaa acaaaatata ggatataatc
     4141 gtctgtactg aggtatttcc cttagctatt catagaggaa cttatttacc tggtaccaat
     4201 atataaaacg atgagaaaac atatgcagac agaaatgaaa aagttattat catatctgaa
     4261 gccatactgg aaagcagcgc tgttagctcc aattctaatg ctgatagaag tgatatgcga
     4321 tttatctcaa cccactttat tagccagcat agtggataac ggtgtagcta aaagtaattt
     4381 accttacata ttaaatactg gtgtccttat gctgggcata gccttaatag gaatggccgg
     4441 cggaatggga tgttcggtag cttccagtat agccagtcag aattttggag ccagtttacg
     4501 ctctgcggtg ttcaaaaaga ttcaacttct ttcgcctctt aacatagata gatttaagac
     4561 ctcatcgctc gtgacccgcc tcaccaatga tataactcaa cttcaaacgg tagttctgat
     4621 gtcgttgagg attatggtac gtgcaccact actttttata ggtggtataa taatggctgt
     4681 atcattaaat gcccgattag cgcttatcct cacggtatct atacccctac tggctgcggt
     4741 tatctattac cgaatgaaaa aaagcacacc gcttttttct tacatgcaag agaagctgga
     4801 tagagtcaat gctgttatca gagaaaatct ttccggcatc cgactgatta aagcctttgt
     4861 gagagccgac cacgaaaaaa aacggtttgc cactgctaac gatgaactca tgtatgccac
     4921 tctccgggct tttaaccttg ttataactat gatgccaata gtgatgctca taatgaattt
     4981 tagtattgtg gcggtgctat ggttcggggg atgggatata gaccggggca atatggaggt
     5041 aggcgagata atagcttttg taaattatat aactcaaata ctcttctcac tcatgatgat
     5101 tggtaacata ttactatttg tttcccgtgc ttcggcttca gcagaaagag tgaacgaagt
     5161 gttagatacg gacatcgata ttaaaaacgc cgataatccg gatttcacac ctctatcttc
     5221 aggagaggta gcttttgaaa atgtgtcttt cgggtatagc ggagataaaa accagttggt
     5281 gctcaaagaa ataagctttc atgccccatc gggtaaaacc atcgctatcc ttggctccac
     5341 cggttcaggt aaatccacac tggtaaacct cataccccgt ttttacgatg ttaccagtgg
     5401 tcaggtgacg gtagataaaa gagacgtgcg ttcaatggat ttagccactt tgaggtctgg
     5461 tatgggtata gtaccgcagg ataccatact attttcaggt actataaaag acaacattcg
     5521 ctggggtaaa gaagatgcca cagatgagga aataatagaa gcagccagag tagctcaggc
     5581 tcatgaattc ataatgagct tccctgaagg ctatgacact cagttaggtc agcgaggtgt
     5641 caacctctca ggaggacaga agcaaagaat agccattgct cgagcaataa ttaaaaaacc
     5701 acccattctc atacttgacg atagcacaag cgccgttgac gtagtcactg aacagcggat
     5761 acaaaaagcg ctgaaggaat ttataagagg ctccactatt tttataatag ctcaacgcat
     5821 aagctcagtg atggatgccg ataaaatatt agtcttaaat gaaggtcgta tagaggcaga
     5881 aggcacccat gaggaattgc taaaaagtag ccccctgtat caggacatat ataattcaca
     5941 actgggagaa ggagttatga tagatgcata acccgggagg agggcgtcac gatgcagttt
     6001 atgtcgtaaa gcccagagat gcgaaaaata ctttaaaaag actatggtgg tatttatcag
     6061 agcacaaaat aagacttttt ctcgtgctct ttcttgtagc cataacatcg gtgttaaccc
     6121 ttgtggggcc ttatctcata gggaaagcca tagataatta tatcattccc cgcgatttca
     6181 atggattatt tcgtctttta atacttatgg cagctatata cgtccttatg tccttattta
     6241 catggttaca gagtcgcact atgattaatg tagcccagct cactatacgt aacatgagaa
     6301 aagatgcctt tgataaatta cagatattac ctgtaagctt ttttgacgcc agaccccggg
     6361 gagatatcat gagcagatta acaaatgata tagaccttat aaacaatgcc ctgagttcca
     6421 gtttaaccca gatattctcc agtattatca ctctgatagg aaccgtaata ttaatgttgt
     6481 ggttaagccc tctcctgaca ggagtcagca tgataacagt acctataatg ttgatcacca
     6541 ccaacattgt aaccaggcac acccgacgat atttttcaga acagcaaaga gttttaggta
     6601 tgttgaatgg ctttgccgag gaaaatatat cgggacaaaa agtagttaaa gcatttgtcc
     6661 gggaggctaa agaaatagag cgttttgagg tcaccaatca agaactaaaa aacgtgggca
     6721 ttaaagctca aatatattca ggcataatgg gacctttaat gaacgtatta aataatatag
     6781 gatttgccat agtagctctg tccgggggat ggctggcagt taaacaaata ataaccatag
     6841 gcaccatagc cgcctttata aactatacca gacaatttac ccgccccttg aatgaattag
     6901 ctaaccagat taataccatc caatcagcca tagcaagtgc tgaaagatta tttgaaataa
     6961 tggatgagcc acctgaacca cctgatcctc ccgatgccat agagctcaaa agcgtcaaag
     7021 gtgacgtaga atttcggaat gtatcattct cttataaatc agatgaagcg gtactcaaaa
     7081 atataagctt ccatgctcat cccggacaaa ccatagctct ggttggcccc accggagcgg
     7141 ggaaaaccag tataatcaat ttgctcgctc gcttctatga ccccgatagt ggggacatat
     7201 tcatagatgg atataatatc cagaaaatta cgagaaaaag tcttcgctca tctttaggaa
     7261 tagtgttaca agatacctac ctcttttctg aatcagtaaa ggataatatt cgttacggcc
     7321 gattagatgc caccgatgaa gaggtaaaag ctgccgctcg attggccaat gctgaacaat
     7381 tcatattaaa cttgcctcag ggatatgaca ctattctctc cgaagatggt ggagacctga
     7441 gtcagggtca gcgccaactg ctggctattg ctcgagccat attagccgac ccggctatat
     7501 tgatactgga cgaagccacg agctcagtag acactcgcac tgagcaacac atccaggaag
     7561 ccatgtacaa actgatgaaa gggcgcacca gttttgtgat agctcaccgg ctcagcacca
     7621 tccgcaaagc cgatatgata ttagtgatag acgatggaga aataattgag agaggcaacc
     7681 atgtgcaact attaaaacag aaaggtttct actataacct gtatatgagc caatttgccc
     7741 atattctgca c
//
LOCUS       DAOPSS010000073         7570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8070_length_7570_cov_10.686494, whole genome shotgun sequence.
ACCESSION   DAOPSS010000073 DAOPSS010000000
VERSION     DAOPSS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8070_length_7570_cov_10.686494"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2009
                     /locus_tag="PKN31_05425"
     CDS             <1..2009
                     /locus_tag="PKN31_05425"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOA99399.1"
                     /translation="RNGELEDFSPAKITKAIYKALAACGKESEEEAEKLTGEVLKLLP
                     ERVKSSIPEVEEIQDTVEEVLMREGFPEVARAYILYREKRRELREAKQKLFGVKDDLK
                     LSLNAVQVLQERYLLRDEEGRIIETPRKMMKRVARAVAEVDARYGKDVDKAYSEFYEM
                     LTSLYFLPNSPTLMNAGTPLGQLSACFVLPVEDSIESIFDALREMALIHKSGGGTGFT
                     FTHLRPRGDRVSTTGGRASGPVSFMRIFDTATEVVKQGGKRRGANMGILRFNHPDIRE
                     FIHAKEKTGFLENFNLSVAITDEEMEKVRKGEELELCNPKDGKVWEKINARELFKMLA
                     QNAWRSGEPGLIFLDEINRHNPTPSQGIIEATNPCGEVPLLPYESCNLGSINLARMVE
                     GGKIDYQRIKETVKRAVHFLDNVIDANRYPLPQVQKMVEGNRKIGLGVMGFADMLAKI
                     GVSYYSEEALKIAEEVMNFISLQARCASQELAEERGTFPNYPESIWKDKNLPLRNATL
                     TSIAPTGSISLIASVSSGIEPFFALAFRRYVLDNTELLEVNPLLEEKLKSYQIPEEIW
                     KVILEKGNLEGVDVPEELHRLFITALEIPPSFHIRMQAAFQKYVDNAVSKTINLPESA
                     TPQDVEEAYLLAHELKCKGITVYRYGSRPQQVLYLGAEKYCEAC"
     gene            2371..3441
                     /gene="thrC"
                     /locus_tag="PKN31_05430"
     CDS             2371..3441
                     /gene="thrC"
                     /locus_tag="PKN31_05430"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOA99400.1"
                     /translation="MRWPGVLEYFADYLPVTSHTPLITLNEGNTPLVESYQLVEKLGA
                     GKLYFKFEGMNPTGSFKDRGMVVAVAKAKEEGRAVVMCASTGNTSASAAAYAQRAGLS
                     CIVLIPQGKIALGKLSQALMHGARVLAVQGNFDDALSIVRYITSHYPVALVNSLNPYR
                     IEGQKTAAFEVVEWLGESPDYLALPVGNAGNITAYWKGFKELKEKGKITSLPRMLGFQ
                     AEGAAPLVSGKPVSNPQTVATAIRIGNPASWKGAIAARDESGGLIEAVSDEEILQAQR
                     MLAEEEGIFVEPASAASFAGVLKKSEEGFFTGRETIVCVLTGHGLKDPDIAIKQGEGR
                     IEEVGASAEEVVEILGLEREHV"
     gene            3434..4333
                     /gene="thrB"
                     /locus_tag="PKN31_05435"
     CDS             3434..4333
                     /gene="thrB"
                     /locus_tag="PKN31_05435"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOA99401.1"
                     /translation="MFEVCVPATSANLACGFDVLGLALNLFYRVEVSSGGEGFHLYHQ
                     GEGEGKIKEEDNLFFHAVRKTWEKASFPGQGMRVKAFNEIPISRGLGSSAACIVAGVI
                     SANEVMGRVLNLSQMIEVASQIEGHSDNVLPAFLGGFTIALLREGRIFYHQLPFLREV
                     EMVVCIPDYTLSTEEMRKVLPSSYPRDKVVFTLSSLAYLLGSIFTGDKEGFFLSLEDE
                     IHQPYRGAKIKGFSEVKEYIQKRNLGNVAISGSGPALILFLSRPLREEEEEDLGKIFQ
                     SRGVSVEIKKVEAVEDGARVRKL"
     gene            4330..5562
                     /locus_tag="PKN31_05440"
     CDS             4330..5562
                     /locus_tag="PKN31_05440"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOA99402.1"
                     /translation="MSLIVQKYGGSSLADLDKIRLVASRVKKCRDEGHELVVVVSAMG
                     KTTDQLISLAKSLNPFPPERELDMLLSTGEQVSISLLSIALSSLGVSSVSLTGGQAGI
                     ITTDTYTKAKIFHIDTRRIKDLLLKHQVVIVAGFQGRTPQGDITTLGRGGSDVTACAL
                     AAALSADFCEIYTDVQGVFTADPHLVPQAKKIARLSYEEMSEMATLGAKVLQPRAVDF
                     SQAYGVRVHVRSTFSEREGTWVEEINSMEEVRVKAITHSGDMLKIVLLGVPNQPGVAA
                     QIFKALSDFSVFIDMIIQSEGREDYKDLAFVASREDEEKVRKAIACLEKELSFQGMKF
                     DETMSRVSLVGAGIASSPNIAYRMFNALARAGINIDMISTSNSRISCLIPRDLMEEAV
                     KVLHEEFIMQEVRLNEEV"
     gene            5549..6565
                     /locus_tag="PKN31_05445"
     CDS             5549..6565
                     /locus_tag="PKN31_05445"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOA99403.1"
                     /translation="MKKFKVAVAGVTGAVGQEMLKVLEERNFPVSELRPLASSRSQGK
                     KVLFRGKEWTVEALSENSFQGVDIALFSAGASVSREFAPLAAQSGCVVIDNSSAFRQD
                     PDIPLIIPEINPHRIKDYSRRNIIANPNCTTTISLMPLKPLHDYGKIKRIIAASYQAT
                     SGAGAEAMAELEEQVRAYVNQEEIVPRVFPQQIAFNVIPHIDTFLDNYYTKEEMKMVW
                     ESQKILEEENLLISATCVRVPVFRAHSVAINIETERKITRGKAIELLSSFPGVKVVDD
                     PEHLLYPTPLMAVGKDECLVGRIREDISSPSGLSLWVVGDQLRKGAALNAVQIAELLA
                     QEIS"
     gene            6638..6723
                     /locus_tag="PKN31_05450"
     tRNA            6638..6723
                     /locus_tag="PKN31_05450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6671..6673,aa:Leu,seq:tag)
     gene            6791..7063
                     /locus_tag="PKN31_05455"
     CDS             6791..7063
                     /locus_tag="PKN31_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99404.1"
                     /translation="MGETVKFPREDYNNFLELFLFEKKAEGRAPRTISDYKLHVSMFF
                     KRFPRTLRSEKDLRASVLEYFSDDIKPTTFSLRREELEYFRILIII"
     gene            7285..>7570
                     /locus_tag="PKN31_05460"
     CDS             7285..>7570
                     /locus_tag="PKN31_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III decarboxylase-like protein"
                     /protein_id="HOA99405.1"
                     /translation="MTKRQVIKIVLDHKQPPYVPWSFGFTKEAADKLIAHYNNSGHQI
                     NSREELEDFVGNHLLKLGSDIGFFEEVGPNLVQDVFGVVWDRSIDKDIGNV"
BASE COUNT         2141 a         1413 c         1897 g         2119 t
ORIGIN      
        1 aaagaaatgg agaattagaa gatttctctc cagcgaaaat tactaaagcc atttacaaag
       61 ctcttgctgc ttgtgggaag gaaagcgagg aagaggcaga aaaactcact ggcgaagttt
      121 taaaactttt accggagcgg gtcaaatcat ctatacctga ggtagaagaa atccaggata
      181 ctgtagaaga ggtattaatg agggaggggt ttccggaggt agctcgagcc tatattctct
      241 accgggaaaa aagaagagag ctgcgagaag cgaaacagaa gctttttgga gtgaaagacg
      301 accttaaact ttctctaaat gctgtgcaag tgttacagga gcgctacctc ttgagggatg
      361 aagagggaag aataatagag actcctcgca agatgatgaa gcgagtagcc agagctgtag
      421 ctgaggtgga cgctcgctac gggaaggatg tagataaagc ttactctgaa ttctacgaaa
      481 tgctgacctc tctctacttt ttgcccaatt cccctacttt gatgaatgcc ggaactcctc
      541 tggggcagct ttcggcttgc tttgttttac cagtagaaga ttccatagaa tccatatttg
      601 atgccctccg ggagatggct ctcatccata aatcaggggg aggtactggc ttcactttta
      661 ctcatcttcg ccctcgaggg gatcgggttt ccacaaccgg tggcagagcc tcgggtccgg
      721 tttcttttat gcgaattttt gataccgcta ccgaagtagt gaaacagggt ggaaaacgcc
      781 ggggagctaa tatgggtatt ttgcgcttta atcatcctga tattcgagaa tttatccatg
      841 ccaaagaaaa aactggcttt ctggaaaatt tcaatctttc tgtggccata actgatgaag
      901 aaatggaaaa agtgcggaaa ggggaagaac tggaactctg taatcccaag gatggaaaag
      961 tatgggagaa aataaacgct cgggaacttt ttaagatgct ggcccagaac gcctggagaa
     1021 gtggagagcc aggtcttata tttttagatg aaataaatcg ccataatcct accccttccc
     1081 agggtataat tgaagccact aatccttgtg gggaagtccc tcttttacct tatgaatcct
     1141 gtaatctggg ttctatcaac ttagctcgga tggtagaagg agggaaaatt gactaccaga
     1201 gaataaaaga aacagttaaa agggcggtac attttctgga taatgttatc gatgctaatc
     1261 gctatcccct tcctcaagta caaaaaatgg tagagggaaa ccggaaaata ggcttaggag
     1321 tgatgggttt tgctgatatg ttagctaaaa tcggagtttc ctattatagt gaggaagcct
     1381 taaaaattgc tgaagaagtg atgaacttta tttctttgca agctcggtgt gcttctcaag
     1441 agctggccga ggagcgaggc acttttccca attatcctga aagtatctgg aaagacaaga
     1501 atttacccct tcgcaatgcc actcttacct ctattgcccc caccggaagt ataagtttga
     1561 ttgcttctgt atccagtggt atagagccct tttttgcttt ggcttttcgc cgctatgttc
     1621 tggataatac agaactcctg gaggttaatc ctcttttaga agaaaaattg aaaagttatc
     1681 aaattccgga agaaatctgg aaggtaattt tggaaaaagg gaacctggaa ggagttgatg
     1741 ttccggagga gcttcaccgt ctctttatta ctgctttaga aattcctcct tccttccaca
     1801 ttcggatgca ggccgctttt cagaaatatg tggataacgc cgtttctaaa accatcaatc
     1861 tcccggaaag tgctactcct caagatgtgg aagaagcata tcttctggct catgagctaa
     1921 agtgcaaagg gattactgtt tatcgctacg gtagccgacc tcaacaggta ctatatctgg
     1981 gagcagagaa gtattgtgag gcttgttaga ggaaggcttt tgaaattctt tcactaagtc
     2041 ccgggcaata tactacccca tttactagtg tagtgtgata caataatcgt accttttggg
     2101 tgcctcggga attcagttat ccattctcgg ggtccttctc ctttcttcaa gtattttccc
     2161 agtattctcc taaagactat gactatattg gacattctga gtcggttaaa cacccagcac
     2221 caaatacagt attagagagc actgagtaga gagatgccat aaggggttta aatctccttt
     2281 actttttaaa tttagtattg aaaactgatg attcagaaga atagttgtgg tatgctaatg
     2341 aaaatctgtt ttggtaaagg aggtttgact ttgcgctggc ctggagtgtt ggagtatttt
     2401 gccgattacc ttcctgtaac atctcatact cctcttatta ctctaaatga gggaaacact
     2461 cctcttgtgg agtcttatca gctggtagag aagttaggag caggaaaact ttattttaaa
     2521 tttgaaggca tgaaccccac cggctcattt aaagaccggg ggatggtggt agcagtggct
     2581 aaagccaaag aagaaggtag agctgtggtg atgtgtgctt ctactggtaa cacttccgca
     2641 tcagctgcag cttatgcgca aagagccggt ctttcctgca ttgttcttat ccctcagggg
     2701 aaaatcgcgc tgggaaaact ctcccaggct cttatgcacg gggcacgagt tctggcagtg
     2761 caaggaaact ttgacgatgc tctttctata gtgagatata taacttctca ctatccggtg
     2821 gctctggtta actcccttaa tccttatcgt attgaggggc aaaaaactgc ggcgtttgaa
     2881 gtggtagagt ggctggggga aagtcctgac tatcttgcgc tacccgtggg taacgcggga
     2941 aacatcactg cttactggaa agggtttaaa gagctaaaag aaaaggggaa aattacctct
     3001 cttccccgaa tgttaggctt tcaagccgaa ggagcggctc ctttagtttc cggaaaacct
     3061 gtttctaatc ctcaaacggt ggctactgct atccggattg gaaaccctgc cagctggaag
     3121 ggagctatcg ctgctcggga tgagtcaggg ggcttgattg aagcagtgag tgatgaagaa
     3181 attttacagg cgcaaaggat gctggctgaa gaagagggga tatttgtaga gccagcttct
     3241 gcagcttctt ttgccggagt gttgaaaaaa tctgaagagg gtttttttac cggcagggaa
     3301 accatagttt gcgttttaac cggacacggg ctcaaagacc ccgacattgc cataaaacaa
     3361 ggtgagggaa gaattgaaga agtaggagca tccgcagaag aagtggtaga aattttggga
     3421 ttggagagag aacatgtttg aagtgtgtgt tccagctacc tcagccaatt tggcttgtgg
     3481 gtttgacgtt ttaggtctgg ctcttaacct tttttatcgg gtagaggtaa gtagcggggg
     3541 agaagggttc catttgtacc atcagggaga gggagaggga aaaataaagg aagaagataa
     3601 ccttttcttt catgcagtca gaaaaacctg ggagaaggcg agttttcccg gacaggggat
     3661 gagagtaaag gcttttaatg agatacccat ttcccggggt ctgggaagca gtgccgcctg
     3721 tattgtagcg ggagtgatat ctgccaatga ggtaatgggg agggtgttga atttatccca
     3781 gatgatagaa gtagcttcgc aaatagaagg acactctgac aatgttttac ctgctttttt
     3841 gggagggttc accatagctc ttctccgaga aggaagaatt ttttatcatc aacttccctt
     3901 tttacgagaa gtggagatgg tagtttgcat ccctgattat actctatcta ccgaggaaat
     3961 gagaaaagtt cttccttctt cttatccccg ggacaaagta gtttttactc tatcatctct
     4021 tgcttatctt ttaggaagta tttttaccgg agataaagaa gggttctttt tatctttaga
     4081 agatgagatt catcagccct atcggggggc taaaattaaa gggtttagcg aggttaaaga
     4141 atatatccag aaaagaaatc tggggaatgt ggctattagt ggctcaggac ctgctttaat
     4201 tcttttttta tctcgacctt tgagagagga agaagaagag gatctgggaa aaatttttca
     4261 atcccgggga gtaagcgtag aaataaaaaa agtagaagcg gtagaagatg gagcaagggt
     4321 gagaaagcta tgagcctcat agtgcaaaaa tacgggggct cttctttagc cgatctggat
     4381 aaaatcaggc tggtagcttc ccgggtaaaa aagtgcaggg atgaagggca tgaattggta
     4441 gtagtggttt ctgctatggg aaaaaccact gatcagctta tttccctggc taaaagctta
     4501 aacccttttc ccccggaaag agagctggat atgttacttt ctaccggaga gcaagtttcc
     4561 atatctcttt tatccattgc tctttcttct ttaggggtga gctctgtttc tttgaccgga
     4621 gggcaagcag gaatcattac cactgatacc tataccaaag ctaaaatttt ccatatagat
     4681 acccggagga taaaagattt gctcctcaaa caccaggtgg tgatagtagc tggttttcag
     4741 ggtagaaccc ctcaaggaga tatcactacc ctgggaagag gagggagcga tgtcactgcc
     4801 tgcgctcttg ctgcagcact gagcgctgat ttctgtgaga tttatactga cgtccaaggg
     4861 gtgtttactg ctgatcctca tcttgttcct caggctaaaa aaattgcccg cctctcttat
     4921 gaagaaatgt cagagatggc tactttgggg gctaaagttt tgcaaccccg agcggtggac
     4981 ttttctcaag cctatggagt gagggtgcac gttcgttcta ctttttcaga aagggaggga
     5041 acctgggtgg aagaaataaa tagtatggaa gaagtgagag taaaggcaat tactcacagc
     5101 ggggatatgt taaaaatagt gttattagga gtacctaatc aaccgggagt agcagcacag
     5161 atttttaaag ctttgtccga tttttcagtt ttcattgata tgattattca gagtgaagga
     5221 agggaagact acaaagactt ggcttttgta gcttctcggg aagatgaaga aaaagtgaga
     5281 aaggccatcg cttgcctgga gaaagaattg tcttttcagg gaatgaagtt tgatgaaaca
     5341 atgagccggg tatctttagt aggagcaggg atagccagca gccctaatat tgcttatcgg
     5401 atgtttaacg ctttagctcg ggcagggata aatatagata tgatcagtac ttctaattct
     5461 cggatttctt gtctcatccc ccgggatttg atggaagaag cagtaaaagt tttgcatgaa
     5521 gagttcatta tgcaggaggt gaggctcaat gaagaagttt aaggtggcgg tagcaggagt
     5581 gactggggca gtggggcagg agatgctcaa agttttagag gagagaaact ttccagtttc
     5641 agagctccgg cctctggctt cttcccgctc tcaagggaag aaagtgcttt tccggggtaa
     5701 ggagtggaca gtggaagcct taagtgaaaa tagctttcag ggagtagata tcgctctttt
     5761 tagcgccggt gcttccgtaa gccgagaatt tgcccctctg gcggcacaga gtggttgtgt
     5821 ggtgatagat aattcctcag ctttccggca agaccccgat attcccttaa tcattccgga
     5881 gattaatcct catcggatta aagattacag ccgaagaaat atcattgcca accccaactg
     5941 taccactacc atttctctta tgcccttgaa gcctttgcac gactatggga aaatcaaaag
     6001 gataatagct gccagttacc aggctacctc aggcgcagga gcggaagcca tggcggagtt
     6061 agaagagcaa gtcagagctt atgtgaatca ggaagaaata gtgcctcgtg ttttccccca
     6121 gcaaattgct tttaatgtga taccccatat cgataccttt ctggacaatt actacactaa
     6181 agaagaaatg aaaatggtct gggagagcca aaaaatactg gaggaggaaa acctcttaat
     6241 cagcgctact tgtgttcggg ttccagtatt tcgtgcccat tcggtggcta ttaatataga
     6301 aacggaaaga aaaataacca gaggcaaagc tatagagctt ttatcttctt ttccgggagt
     6361 gaaggtagta gatgatccag aacaccttct ttatcctact cctttaatgg cggtaggaaa
     6421 agatgaatgt ttagtgggaa gaataaggga agatatttcc tctccttcgg gtttaagtct
     6481 ctgggtggtg ggagatcaac tgagaaaggg agcagcgcta aacgcggtgc aaattgctga
     6541 acttttagct caagaaattt cttaaagttt tgctctctat agagccaagt taccaggggt
     6601 tgtcagaaaa tataaattgt cttaaaattt cagtgttgcg agagtggcgg aatggaagac
     6661 gcgctggact taggatccag tgggtgaaac cccgtgcggg ttcaactccc gcctctcgca
     6721 ccagaagcgc gtctcttctg tttctctttc taaaaagctc ttttatagtc gatggactac
     6781 ctcatttctc atgggagaaa cagtaaaatt tcctcgagaa gattataata attttttgga
     6841 gctatttctg tttgaaaaga aggctgaagg aagagctccc cgcactattt ccgattataa
     6901 gctccacgtt tcaatgttct ttaagcgctt tcctcgtact ttacgttcag aaaaagattt
     6961 aagagcttcc gttcttgagt atttctccga tgatattaag cccactactt ttagccttcg
     7021 tcgagaagaa ttagaatatt ttagaatact tattatcatt tgatcccgat ttttttagca
     7081 aaatataact tgcttttaaa gtttgagtgg tttaagaggt tttggaggat actgaaatta
     7141 ataaccagtt gagtgggagg gtaatactat tggtgggttc ctaactttag aagaggtttg
     7201 aataatcgaa cgtcagaggt tattatatat caggttcgag tttaatagag gtgaggagcc
     7261 cggtattgga agggggagtg cgttatgacc aagcgccagg taataaaaat tgtacttgac
     7321 cacaaacaac cgccttatgt tccctggtct tttggtttta ccaaagaagc ggcggacaaa
     7381 cttattgctc attataataa ttccggtcac caaatcaatt ccagggagga actggaagat
     7441 tttgttggca atcatctttt gaagttggga agcgatatcg gttttttcga agaggtgggg
     7501 ccaaacttag tgcaagatgt ttttggagta gtctgggacc ggagcataga taaggatatc
     7561 ggcaatgtcc
//
LOCUS       DAOPSS010000074         7489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8175_length_7489_cov_7.864407, whole genome shotgun sequence.
ACCESSION   DAOPSS010000074 DAOPSS010000000
VERSION     DAOPSS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8175_length_7489_cov_7.864407"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PKN31_05465"
     CDS             <1..930
                     /locus_tag="PKN31_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOA99406.1"
                     /translation="IPKRMLPDKILKSSDIVGKLNAKFARETGLQEGTPVVSGGIDAP
                     VAQLSAGVLSEGEHVAMAGTSMCWGTVHGGQYLTPLLVSFPYVVYDDKLIYTFGGSAT
                     SGGLARWFRDQFGKYEKEVEKNTGISAYTLLELQTQDIPPGSEGVVVLPYFMGERSPI
                     WDPDARGTIFGLSLYHSRAHVYRAMLEAAAYSLRHNMEEGIKAGLKLDPECWIVGGVA
                     RSSFWVQIFADITGFDMKRLSKDVEAPFGDAFLAGLGTGVIDKPERIKEWVDFRPTIK
                     VNQDNHHIYDQYYSIYRELYENTKATMKKLADI"
     gene            945..1622
                     /locus_tag="PKN31_05470"
     CDS             945..1622
                     /locus_tag="PKN31_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOA99407.1"
                     /translation="MNLDALYALNCGMYILCSGWEGKFNGLIVNALTQVTAFPPQLVV
                     SVNKESLTSTYIQKSGYFSVSVLEKNTPLPLIGLFGFRSGRDTDKLSQVKHRLGKTGM
                     PIITEHTVAYLEAKVVNELDAGTHFVFLGEILETDFTEKGGEPMTYSYYRDIKGGRVP
                     RTAATYHEETKEKERYRCQVCGYIYDSSFGDPDSGIKPGTLFSDLPPQWVCPICGASK
                     DQFEKIQ"
     gene            1641..2807
                     /locus_tag="PKN31_05475"
     CDS             1641..2807
                     /locus_tag="PKN31_05475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOA99408.1"
                     /translation="MRNFDYCNPTRIIFGKGETDRVGKECQKLGKKVLFIYGGGSIKQ
                     NGLYDRIMKHLQEEKLVVYELGGVKPNPRIDKVREGVAICKKEKIDLVLAVGGGSVLD
                     TGKIVAAGAIYPGDPWDFFIDEHKIPGKIPLVTVLTLAATGSEMNHNAVITNEETLEK
                     LGTHNPHLYPTLSILDPENTYTVPRDQTVNGIVDMMAHVFEQYFHPFPETPLQDRWAE
                     GLMKTIIEYTPRVLQNPTDYDGRAVIMWCGTMALNHLLEEAGGGGEWTCHPIEHVLSA
                     VYDIPHGAGLAIIFPQWMKYVLDVIPHKFAQFAERVWEIPRANKSDQELGLAGIERTR
                     EWFREIGAPTTLREAGIGEEKLTEMARMAVKSGNLGVTKVLDEKDVEEILRMSL"
     gene            complement(2790..3962)
                     /locus_tag="PKN31_05480"
     CDS             complement(2790..3962)
                     /locus_tag="PKN31_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA99409.1"
                     /translation="MDTRNHHFISQWGLSHAVNDIYWFVLPSILPLLLEQFGLSYKSA
                     GVILTLYLGAIALFSFIFGKLTDSLSPFTLIGGGFLIASLGFFLLGWAGNVFVFIFFL
                     FVAASGVSTYHPAAYALVSDSTDKQKGTQLGNFEFWGALGIFIMFLLYGALLQKISWR
                     GILYVTAFPGIIIGYCFLKIFPRPGKEKQSHFSSSPRETGSSLSLGVQILFFLALALR
                     ILSITAIVNFAPTYLVKERAFSPSSANLVTGVLFLGGMLATLVLGRLVDRFPPFPFIL
                     WISALTIPIIWVLAYVQNAIFLMILFFLLGSCWLGFMPAQNYILSSFSSQGKGQIFGL
                     MMAATTLTNAFAPGLFGLVADRIGISSSIRFFSLFVALSFLIFLLLFPHFSLQRHS"
     gene            4066..4434
                     /locus_tag="PKN31_05485"
     CDS             4066..4434
                     /locus_tag="PKN31_05485"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOA99410.1"
                     /translation="MAIDKEQLSLLIEEILEELPPLFKNSISNVEFVVEDYPDPEIQK
                     QFGSRFLLGLYQGIPLPRRNRSYNLVMPDRIVIYLGNLERVAGKNWPEEVKKVLWHEI
                     GHYFGLNDEELWKLSSEEKK"
     gene            complement(4742..5323)
                     /locus_tag="PKN31_05490"
     CDS             complement(4742..5323)
                     /locus_tag="PKN31_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA99411.1"
                     /translation="MYEEKSDREIITEVLNGNDDLFYFLVRRYEKQILNYIYRLVKQK
                     EEAEDLAQETFTKAFFALHQYNHSYEFSTWLFRIALNVCRDYFRRRKILFFSLNTPVG
                     EEEETEWGELIEQNSFLDPDGELDNRELRRELEKAIDNLPLKFKEVIVLRHLEGLSYS
                     DISEITNLPVGTVKTYLHRARKKLQKELKEYLD"
     gene            complement(5334..6521)
                     /locus_tag="PKN31_05495"
     CDS             complement(5334..6521)
                     /locus_tag="PKN31_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA99412.1"
                     /translation="MREKIIFLGVSLVLVVGIFSGCLSVTEKGSLEGEVWGESEPLVG
                     AVVEGGGCTVFTGEGGKFLIEGLSSGEHYFFFSYPEYTGKVIKAEVKAGETRKISQEG
                     RVYLVPEDEESLNNYIIELYSLGFYERALSQADLFLANYPGGNSLPQVLFIKGASFYY
                     LGDFQNAISFLSYVRDNYPNNEFSDDAQYLLAKSFGEGLKDYGRAITEYEWLVNNYPG
                     SEFLGVAYFEMGDCYYILGFYQEAYSNYDKAQSYGGEAGRKALYSAAHCLYKLENYPE
                     AALLFSKYVELYPQTDISDDAQYFVGAAWYKAEEYSKALVAFQNCVNNYPQGTWYNGI
                     LIAPAALLNQGLCLEKLGRYQEAYNIYLRIIREYPGAKWADGSSLIKNVQFRIEWLKN
                     NIL"
     gene            complement(6521..7195)
                     /locus_tag="PKN31_05500"
     CDS             complement(6521..7195)
                     /locus_tag="PKN31_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA99413.1"
                     /translation="MKKVSFYLMVLVLVISCSGGWLRAEEKNLFDQAIEARGRGENQK
                     ALKILERCVEEGIEVHRAYFEMGKILLEQGKYRRAISVSEKAIKAYEEYLEAHPEDHS
                     AWWELGYIYEVRSDYSKEWDKAQEALEKAVELSPSNSFYLLHLGWVYYKKGDSEEAEE
                     AFRKILNKNPKNFQARYFLAATLIEEGRKEEAKEELTYILEESSKEEIAYNWAEEKLK
                     ELKGES"
     gene            complement(7204..>7489)
                     /locus_tag="PKN31_05505"
     CDS             complement(7204..>7489)
                     /locus_tag="PKN31_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOA99414.1"
                     /translation="TENFEVAYHLKYSIPFSALVFMLMGIPLGVQRTKDTRTIGVIVT
                     VILAFAYYMLLSIFRSLGRGGIMEPMIAAWMPNIIFGLPGLILYLTVDRK"
BASE COUNT         2421 a         1576 c         1456 g         2036 t
ORIGIN      
        1 attcctaaaa gaatgctccc tgataaaatt ttaaaatcct ctgatatagt aggaaaactc
       61 aatgctaaat ttgccagaga aactggcctg caggaaggga ctccagtcgt atcaggagga
      121 attgatgctc cagtagctca gctttccgcc ggggtgctat ctgaaggaga acacgtagct
      181 atggctggaa cttcaatgtg ctggggaacg gtgcatggag gacaatatct gactccccta
      241 ttagttagct ttccttatgt agtttatgat gataagttga tttacacttt tggagggagt
      301 gccacctctg gaggactggc ccgctggttc agggatcaat ttggaaaata cgagaaagaa
      361 gtagaaaaaa ataccggcat atcagcttac accctattag aactacaaac tcaagatatt
      421 cccccaggaa gcgaaggagt ggtggtttta ccttatttca tgggtgagcg ctcacctatc
      481 tgggatcccg atgcccgagg aactattttt ggattgtctc tttaccacag tcgagcccat
      541 gtctatcgag caatgttaga agcagcggct tactccttgc gccacaatat ggaagaagga
      601 ataaaagcgg gcttgaagct tgatccggag tgctggatag tagggggagt cgctcgctct
      661 tcgttctggg tacaaatttt tgccgatatc actggcttcg atatgaaaag actttccaaa
      721 gatgtagagg ctccctttgg tgatgctttc ctggccgggc tgggaacggg agtaatcgat
      781 aaaccagaac gaatcaagga gtgggtagac tttcgtccaa caattaaagt aaaccaagat
      841 aaccatcaca tttatgacca atattactcc atttatcggg agctttatga gaatactaaa
      901 gcaactatga aaaagttagc cgatatatag aaaggagagc aaacttgaat ttagatgctc
      961 tttatgctct aaattgcggg atgtacattc tatgttcggg atgggaagga aaattcaacg
     1021 gtcttatcgt aaatgctcta acccaggtaa ccgccttccc tcctcaactg gtggtgagtg
     1081 taaacaaaga aagtttaacc tctacctata tacagaaaag tggctatttt tcagtatcag
     1141 ttctggaaaa aaacactccg ctacctctca ttggcctctt tggcttccgc agtggtagag
     1201 atactgacaa attatctcaa gttaaacacc gcctgggtaa gacaggaatg cccatcatca
     1261 ctgaacatac agtggcttat ttagaagcca aagtggttaa tgagttagat gctggtactc
     1321 actttgtgtt tctgggagaa atattagaaa ccgattttac cgaaaaaggt ggagaaccaa
     1381 tgacttatag ttactatcga gatataaaag gaggcagagt ccctcgcacc gctgccactt
     1441 accacgaaga aaccaaagaa aaagagcgtt atcgatgtca ggtttgtggc tatatctatg
     1501 attcttcttt tggtgacccc gattcaggga taaagcctgg aactctgttt tccgacctac
     1561 ctcctcagtg ggtgtgtccc atctgtggag caagtaaaga tcagtttgaa aaaattcagt
     1621 aaaattgagg aggcacaaaa ttgagaaatt ttgattactg caatcccacc agaataattt
     1681 ttggtaaagg agaaactgac cgggtaggga aagaatgcca gaagctgggg aaaaaagttc
     1741 tctttatata tgggggagga agcattaaac aaaatgggtt atacgataga ataatgaaac
     1801 atctgcaaga agaaaaactt gtcgtttatg agttaggagg agttaaaccc aacccccgta
     1861 ttgataaggt aagggaagga gtagctatat gtaaaaagga gaaaatcgat ctggttttag
     1921 cggtgggagg aggaagtgtt cttgataccg gaaaaattgt agctgcaggt gctatctatc
     1981 cgggagaccc ctgggacttt tttatcgatg agcataaaat cccgggtaaa atacctctgg
     2041 ttactgtttt gactttagct gctactggtt cagaaatgaa tcacaatgcc gtcatcacca
     2101 atgaggaaac tctggagaaa ctcggaactc acaatcctca cctctatccc accctttcca
     2161 ttcttgatcc ggaaaatacc tacaccgttc ctcgagatca aaccgtaaac ggtatcgtcg
     2221 acatgatggc tcatgttttc gagcagtatt ttcacccttt tcctgaaacc cccctacagg
     2281 accggtgggc agaaggattg atgaaaacca tcattgaata cactccccgg gttttacaaa
     2341 accccactga ttatgatggg cgagcagtaa ttatgtggtg tggtactatg gctctcaacc
     2401 atcttttaga agaagcagga ggaggcggag agtggacctg ccaccctata gagcatgttt
     2461 tgagtgcagt ttatgacatc cctcacggag caggattagc tataattttt ccccagtgga
     2521 tgaagtatgt cctggatgtt atccctcaca aatttgctca gtttgcagaa agagtctggg
     2581 aaattcctcg agccaataaa agcgaccagg aattgggact ggcaggaatt gaaagaaccc
     2641 gagaatggtt tcgagaaatt ggcgctccca ctactttaag agaagcagga attggcgaag
     2701 aaaaattaac agaaatggcc agaatggcag tcaaatcagg aaatttaggg gtaacaaaag
     2761 ttttagatga aaaggacgtg gaagagattt taagaatgtc tctgtaaaga aaaatgggga
     2821 aagagcaaaa gaaaaattaa gaaacttaga gctacaaaca aagaaaaaaa cctgatagag
     2881 gaactaattc ctatccggtc ggctactaaa ccaaaaagac caggagcaaa tgcattagtt
     2941 aaagtggtag cagccatcat caaaccaaaa atttgccctt tcccctgaga agaaaaagaa
     3001 gagagaatat agttctgggc aggcataaat cctaaccagc aactccccaa caaaaagaag
     3061 agaatcatca aaaaaatagc gttttgtaca taagcgagaa cccatatgat aggaatggta
     3121 agagcagaaa tccatagtat aaaagggaaa ggtggaaagc ggtctactaa cctacccaga
     3181 actaaagtag caagcattcc ccctaagaac aatacaccag taactaaatt ggcagaagag
     3241 ggagaaaaag ctctttcttt aacaagataa gtaggagcaa aatttactat agcggttata
     3301 cttaaaattc tcaaagctaa agccagaaaa aaaaggattt ggactcccaa agaaagagaa
     3361 gatcctgttt cccgaggaga agaagagaaa tgagattgtt tttcttttcc tggtctggga
     3421 aatatcttga gaaaacaata gccaattatt attccaggaa aggcagtaac ataaagaatt
     3481 cctctccagc ttattttctg caagagagct ccatagagaa gaaacataat aaaaattccc
     3541 aaagctcccc agaactcaaa gtttcctaat tgagttcctt tttgtttatc ggtactatca
     3601 cttactaaag cgtaagctgc cggatgataa gtactcactc ctgaagcggc gacaaaaaga
     3661 aagaaaataa aaacaaagac attacctgcc catcctaaaa ggaaaaagcc taaagaagct
     3721 atcaaaaatc cccctcctat gagagtgaaa ggagaaagag aatcagtaag ttttccaaaa
     3781 ataaaagaaa agagagcaat agctcctaaa taaagggtaa gaatgacacc agctgattta
     3841 tagctcaatc caaattgttc taaaagaagc ggcaaaatag aaggtagaac aaaccagtaa
     3901 atgtcattta cagcatggct cagaccccat tggctaataa aatggtgatt tctagtatcc
     3961 attctttttc tttaaatctc cctggttctc aattatatca gattatccat tccttatgag
     4021 gtataatttt aatattctga tagagaaagg aggaaaaaga ctaccatggc aatagacaaa
     4081 gaacaacttt ccctcttaat agaagaaatt ctggaagagc tccctcccct tttcaaaaat
     4141 agtataagta atgtagaatt tgtagtcgaa gattacccgg atcccgagat tcaaaagcag
     4201 tttgggtctc gctttttact aggactttat cagggtattc ctcttcctcg aaggaataga
     4261 agttacaacc tggttatgcc cgaccggata gtaatttatc taggaaatct ggaaagagta
     4321 gccggtaaaa attggccaga ggaagtaaag aaggttctgt ggcacgaaat cggacattat
     4381 tttggcttaa atgatgagga actctggaaa ttgtcaagcg aggaaaaaaa gtaaataatt
     4441 tttcctgata cctgaaagaa gattaatacc tgagtttatg ttaaatttgg ggtatgagag
     4501 tattccgtta tttgtctcga taagaaaaag cctcaaagaa agaataccaa agtaaactaa
     4561 actggcctgg aaaattcttt tcagtgactc ctgcattgta aataaatgac tcgtcacctc
     4621 catcacctct tgcactgtaa tcaaaaagct ttcaatcttc gtcttagctt tggctctctc
     4681 ctattattcc aactagctca ttgtggtcca ttctccagcc tgctagacca ctttccctcg
     4741 cttaatccag atattctttt agctcttttt ggagtttttt acgagcgcga tgaagataag
     4801 tttttaccgt acccaccggt aaattggtaa tttcagaaat atcactataa gataaacctt
     4861 ctaaatgacg caagactatt acttctttga atttaagagg aagattatca atggcttttt
     4921 ctaattccct ccgtaattcc cggttatcca attctccatc agggtcaaga aaggagtttt
     4981 gctctattaa ttctccccac tctgtttctt cttcttcccc caccggggta ttaagagaaa
     5041 aaaagagaat ttttcttcgc cggaagtaat ctcgacaaac attaagggca atccgaaaaa
     5101 gccaggtaga gaactcatag gaatgattat attgatgaag agcaaaaaaa gccttggtaa
     5161 aagtctcttg agccaggtct tctgcttctt ctttctgctt tactaaacga tagatataat
     5221 ttaaaatttg cttttcgtaa cggcgcacta agaaataaaa caggtcatca ttaccattca
     5281 atacctcggt aatgatttcc cgatcacttt tttcttcata catgttagtt aatctacaaa
     5341 atgttatttt taagccattc aatacgaaac tgaacgttct tgattaaaga gctaccatcg
     5401 gcccacttgg ctccggggta ctcccggata attcgaagat aaatgttgta agcttcttga
     5461 tatctgccca gtttttccag acaaagccct tgattaagaa gggccgccgg agcaatgaga
     5521 attccattgt accaggttcc ctgtggataa ttattaacgc aattttgaaa ggctactaag
     5581 gctttactgt attcttcagc cttataccag gcagcgccga caaagtattg agcatcatct
     5641 gatatatcgg tctgaggata gagctctacg tatttggaaa aaagaagcgc cgcctccggg
     5701 taattttcca atttatagag acaatgagca gcagaataga gagccttcct tcctgcttca
     5761 ccaccatagc tctgagcttt atcataatta gaataggctt cttgataaaa acctaaaatg
     5821 taataacaat cccccatctc aaagtaagct acgcctaaaa attcacttcc tggataatta
     5881 ttcaccaacc attcgtattc tgtgatagcg cgtccatagt cttttagccc ttcgccaaaa
     5941 cttttagcta agagatactg tgcatcatca gagaattcat tattcgggta attgtctcta
     6001 acataagaca aaaaagaaat ggcattctgg aaatccccca ggtaataaaa ggaagctcct
     6061 ttaataaaaa gaacctgagg tagtgaattt cctcctggat aattagcaag gaacaaatcc
     6121 gcctgagaaa gggctcgctc ataaaaaccc agactataga gctcaataat ataattatta
     6181 aggctttcct catcctcggg gactaaatat accctacctt cctgactaat ctttctcgtt
     6241 tctccagctt ttacttccgc ctttatcact ttacccgtat actcaggata ggaaaagaaa
     6301 aagtaatgct ctccagacga caacccctct ataaggaact tccccccctc accagtaaac
     6361 actgtgcatc ctcctccttc taccactgct cccacgagag gctcgctttc tccccaaact
     6421 tccccttcca gggaaccttt ctctgtaacc gaaagacagc cggaaaaaat accaaccact
     6481 aagactaaac ttactcctaa gaagataatt ttttccctca tcaagattcc cccttcaatt
     6541 ctttcaattt ttcttctgcc caattatacg ctatctcctc tttagaagat tcttctaaaa
     6601 tataagttaa ttcttcctta gcctcctctt tccttccttc ttcaatcaat gtagcagcta
     6661 aaaaataacg agcctggaaa tttttagggt ttttattcag gatctttcta aaagcttcct
     6721 cagcttcctc actgtctcct tttttatagt aaacccaacc caagtggagg aggtaaaaag
     6781 aattagaagg agaaagctct accgctttct ccaaagcctc ttgagcttta tcccactcct
     6841 tggagtaatc acttctaacc tcataaatat aacccagttc ccaccaggcg ctatgatctt
     6901 caggatgagc ttccaaatac tcttcataag cctttatggc tttctcagaa acagagatag
     6961 ctcgccgata ttttccctgc tccaggagta ttttccccat ctcaaagtaa gctcgatgca
     7021 cctctattcc ttcttctaca caccgctcta agattttcag tgctttttga ttctctcctc
     7081 ttccccgggc ttctatggcc tggtcaaaaa ggtttttttc ttcagctctt aaccatcctc
     7141 cactacagga aatgaccagc accaatacca tcagataaaa agagaccttt ttcattttat
     7201 acctcacttc ctgtccaccg tcaaatatag gataagacca ggcagaccga aaataatatt
     7261 gggcatccag gctgctatca taggctccat tatccctcct cttcccagag aacgaaaaat
     7321 ggagagcaac atataataag caaaggctaa tataacggta acgataactc ctatggttct
     7381 tgtatcttta gtacgttgaa ctcctaaagg tattcccatc aacataaaaa ccagagcaga
     7441 aaaaggaata gaatacttaa gatgatacgc tacttcaaag ttttcagtt
//
LOCUS       DAOPSS010000075         7395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8300_length_7395_cov_8.654360, whole genome shotgun sequence.
ACCESSION   DAOPSS010000075 DAOPSS010000000
VERSION     DAOPSS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8300_length_7395_cov_8.654360"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            435..1487
                     /gene="nusA"
                     /locus_tag="PKN31_05510"
     CDS             435..1487
                     /gene="nusA"
                     /locus_tag="PKN31_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOA99415.1"
                     /translation="MSKEIFAAIEQIEKTKGIPRETLKEAIEAALLSAYRKSFKSSQK
                     GVSVILDPQDGSIKVFARKTVVDKLKDSTTEILEEEAKRLGIDARVGETVEIEVTPQD
                     FGRIAAQTAKQVIVQRIREAERDLIYAEFVEKQGEIITGIISKKEGSNIIVDLGKTEG
                     LLPFSEQVPTENYRIGGRMKFYIVEVKKTSKGSRIVLSRTHPGLVKRLFELEVPEVHD
                     GLVDIKAIAREPGVRSKVVVESRSDKIDPVGSCIGNRGSRVKNITDELRGEKIDIIRW
                     SNDPRLLIAEALSPARVVKVELDEETDTARVFVPEDQLSLAIGKDGQNARLAARVTSW
                     RIDIKGEKDIGQEDSH"
     gene            1465..1755
                     /locus_tag="PKN31_05515"
     CDS             1465..1755
                     /locus_tag="PKN31_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOA99416.1"
                     /translation="MARKIPIRTCIGCRERKEKKALIRLVKGEEGIEIDPTGKKAGRG
                     FYLCPCRECIDKLNLKNLSHALRVEVREEELKKLKEVLGSYVESQEKEVDNV"
     gene            1748..3679
                     /gene="infB"
                     /locus_tag="PKN31_05520"
     CDS             1748..3679
                     /gene="infB"
                     /locus_tag="PKN31_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOA99417.1"
                     /translation="MSKIRVYKVAENLGMPTKEVVDLLKELGVEVKNHMSNVDEELVE
                     LLQEEISYQKKKEEEARKKQEKTLSFDSLPTLREVAKIFNEEVEELILELADLNMVFG
                     PKDFLPPLVVERLARERGWYVKWEKDLKELTHKMLDTKTYPRPPVVTILGHVDHGKTT
                     LLDAIRSSQVAETEFGGITQKIGAYQVEIDGRKITFIDTPGHEAFTTMRARGAQATDI
                     AVLVVAADDGVMPQTVEAIQHAKAANVPIIVAINKIDKVGANPERVKQQLAEYDLIPE
                     EWGGETIFVNISALQKKGISDLLDLILLQADLLELQARHTGYATGVVIESRLDRGRGP
                     VATVIVQEGILKVGDYFVAGSSWGRVKSLLNYLGKTVREAYPSTPVEVVGFSDLPPAG
                     TKLMVVGEEKIAHLIAERRKEREKEKLLKESRPYSISLEEMLAPEEGKSRELNLILKA
                     DFQGSLEALEKAIASLEEEEVKINIVYQGVGNITETDIMLASASQGIVIGFNVRLLSE
                     AAKIAKREGVEVRLYRVIYEVIDDIEKAIKGLIEPRLVEETLGRAEVRAIFKIPRVGE
                     IAGSYVTQGKIERGALARILRGEEVIKEGAKITSLKRFKEDVREVTQGYECGIGLENF
                     DDFQEGDILEAYVIKEEEK"
     gene            3676..3957
                     /locus_tag="PKN31_05525"
     CDS             3676..3957
                     /locus_tag="PKN31_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOA99418.1"
                     /translation="MRVGACQIDIFINESFSLKDKRRVVNSLKERLRNRFNVSVIEIS
                     TNSRWRKSSLGISCVGNDERSVQLLLQQVVQFVEGDDRLEITNLTIEIY"
     gene            3994..4359
                     /gene="rbfA"
                     /locus_tag="PKN31_05530"
     CDS             3994..4359
                     /gene="rbfA"
                     /locus_tag="PKN31_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOA99419.1"
                     /translation="MKEQRVERVAELIKREVSIILLSRVRDPRLKRFTITRVKVSQDL
                     RQAKVFIYFPEGDKEEKYQALQKATGFIKSELALRLRIKFMPEIVFVIDDTMEKAFQV
                     VELLNQMERKEDGSEKGDN"
     gene            4334..5365
                     /locus_tag="PKN31_05535"
     CDS             4334..5365
                     /locus_tag="PKN31_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOA99420.1"
                     /translation="MEAKKEIIEAFQLHNHFIIISHQNIDGDGLGSMLATFFFLKEEG
                     KESFMVQDEAIPYFYEFLPGKEELLTLEEFYSLNSSSERDVVIVLDCSHPDRLGRLKE
                     LCNGASLLVNIDHHPDNARFGDINWVVPDSPSSTLLVYELIKEAGVKISSDMAINLLT
                     GFVTDTGGFQFVDMDSRLLQVVEDLINSGASLAQIMRYAFHFRRLEALKLLGRALDHL
                     FYDEDFRFSVIYLTKEDFEACGAQEEDSEGIVDYGLYIPGAELSLFFKEVEDGSFRVS
                     LRSQEERDILPIAHYFGGGGHHKAAGFKIEGDFPRVKEEVLSVVRKFLSNPSSLTEEV
                     KKESAGRNT"
     gene            5346..6218
                     /gene="truB"
                     /locus_tag="PKN31_05540"
     CDS             5346..6218
                     /gene="truB"
                     /locus_tag="PKN31_05540"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOA99421.1"
                     /translation="MPGGILNVYKPVGMTSFEITERVGKILGEKAGHTGTLDPFARGV
                     MVVCWGKATRLASFFQALDKTYRARIRFGISTDTYDVTGKIVKVRKGNLSKKEGEELL
                     LKYRGAIKQQVPPFSASKHRGKPRYRYARQGKTLPVQEKEVEIYDLELISWEEGEWPE
                     AEILVRCSSGTYIRSLAHEWGEDSGLGAYLFSLRREKVGDFSLEQSEEITEREIDRQF
                     LLSKSIPPDQGLYWITSVHLEEKEAKRIKHGGFVFSPWQKKGWVKMYEGDKFLGLGKI
                     EDDGELRPRIILGE"
     gene            6221..7204
                     /gene="ribF"
                     /locus_tag="PKN31_05545"
     CDS             6221..7204
                     /gene="ribF"
                     /locus_tag="PKN31_05545"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOA99422.1"
                     /translation="MWQRGIKSAENNILALGFFDGVHRGHQYLLGEAYRLAQEEKAKL
                     HIFTFYPHPRNFLSSEEVKHILTYREKYCLIQELFPQSLLQFVRFNKSFREMTAQEFL
                     TLVEQEIRPRGVVVGENFRFGFQAQGDAQLLKNHLSFRGIEVTVVPSLREGGELISSS
                     RIRWHLESGDLLSANRLLGYSFFLEGRVRRGKGVGRKLGFPTANLYPPSYKLLPASGV
                     YTGYVSGKEIGERNPSLIYVGHRPTVNFSPRRLVEVFIPEVSRELYGERLTIEFQAFL
                     REEMVFPSLSELKNQIEQDLEALRITTEQSAISPFHHCEHGAERGEQATFS"
BASE COUNT         2257 a         1310 c         1889 g         1939 t
ORIGIN      
        1 taagggcaaa aaacatggat tcccacctcc tcgtcacccc ggcaatcctc aagccggggt
       61 ccacagtatt aaaaaccttt ggatgcctga taaaagactt caggcatgac gaaagagggg
      121 gattcccgcc tgaatttcag gcatgacaaa aaggaaacct tcggctcttt ttctttccct
      181 cgctcttcca gagagaaagg caggagggtg tggaggctat cgcagtaaga aggtttagac
      241 tgataagaga ggggaaaata atggtgcctc tgcgatgggt tgacaccatc tgcaaccctc
      301 aaaaatttgc actatttaac gatttgagtt aaaatagtag cagtcgaaaa gtgctgacaa
      361 gaaagagtgg gtcttcccac tctttcttgt ttataagttc tttatacttg gaaaatcctt
      421 ttagggttgg taatatgagc aaagaaattt ttgctgctat tgagcaaata gaaaaaacta
      481 aaggcattcc tcgagagaca ctgaaggaag ccattgaggc tgctcttctt tctgcctatc
      541 ggaagagctt taaaagctcc cagaaggggg tttcggttat tcttgatccc caggatggta
      601 gcataaaagt ttttgctcga aaaacagtgg tagataaatt gaaagactct accacagaaa
      661 tattagaaga agaagctaaa agactgggaa ttgatgcccg ggtgggagaa acagtggaaa
      721 tagaggtgac tccccaggat tttggaagaa ttgctgctca aacagctaaa caggtgattg
      781 tgcagagaat aagagaagca gaaagagact tgatttatgc cgagtttgtg gaaaaacaag
      841 gagaaattat aaccggtata atctctaaaa aagaaggtag caacatcatt gtggatttgg
      901 gcaaaaccga aggcttactt ccttttagtg agcaagttcc taccgaaaac taccggatag
      961 gagggagaat gaaattctac attgtagaag tgaagaaaac cagcaagggt tctcgaatcg
     1021 ttctttctcg tactcatcct ggtctggtga aaaggttgtt tgagctcgaa gttccagaag
     1081 tacacgatgg tttagtagat ataaaagcca tcgcccggga accgggtgtg aggagcaaag
     1141 tggtggtgga aagtcgaagc gataaaatag acccggtggg gtcttgcata ggtaaccgcg
     1201 gctctcgagt gaagaatatc actgatgagt tgaggggaga gaaaattgat attataaggt
     1261 ggagcaatga cccccggctt ctgatcgccg aggccttgag ccctgctcgg gtggtaaaag
     1321 tagagttaga cgaggaaact gatactgccc gggtttttgt gcccgaagac cagctgtctt
     1381 tagccattgg taaagatggg caaaatgctc gtctggctgc tcgggtaacc agctggagga
     1441 ttgacatcaa aggggaaaaa gacattggcc aggaagattc ccattagaac ctgtatcggt
     1501 tgtcgagaaa ggaaagaaaa aaaggctttg attagattgg ttaaagggga agagggtatt
     1561 gaaatagacc ccacggggaa aaaagcagga agaggatttt atctctgtcc ctgcagggaa
     1621 tgtatagata aactgaacct taaaaatctc tctcatgctc tgcgagtaga ggtgagagaa
     1681 gaagaattaa agaaactaaa ggaggtattg ggtagctatg tagaatccca ggaaaaggag
     1741 gtagacaatg tctaagataa gagtttacaa agtagcagaa aacttaggaa tgcccaccaa
     1801 agaggtggtt gatttactta aagagctggg agtagaagtt aaaaaccata tgagcaacgt
     1861 tgatgaagaa ctggtcgagc tattgcaaga agaaataagt tatcaaaaaa agaaagaaga
     1921 agaagctcga aaaaaacaag aaaaaactct tagctttgat tccttaccta ctttaagaga
     1981 agtagcaaaa atatttaacg aagaggtaga agagcttata ctagaattgg ctgatttaaa
     2041 tatggtcttt ggtcctaaag actttctccc tcctcttgtg gtggaaagac tggcaagaga
     2101 aagaggctgg tatgtgaagt gggaaaaaga cctgaaagag ttgacccaca aaatgctgga
     2161 tactaaaacc tatcctcgcc ctccggtggt tactattctg ggtcacgttg accatggtaa
     2221 aaccactctt ctggatgcta tcagaagctc tcaggtggca gagactgaat ttggcggtat
     2281 cactcagaaa attggtgcat accaggtaga gatagatggg agaaaaatca ctttcatcga
     2341 cactcccggt catgaagcat tcactaccat gcgagctcga ggagcacagg ctaccgacat
     2401 cgcagtttta gtggtagccg ccgatgatgg ggttatgccc cagaccgtgg aagctatcca
     2461 gcatgcaaaa gcggccaacg ttcccattat cgtggccatt aataaaatag ataaagtagg
     2521 agccaacccg gaaagagtaa aacagcaact tgctgaatat gacttaatcc ccgaggaatg
     2581 gggaggggaa actatttttg tgaacatttc tgctcttcag aaaaaaggaa tttctgacct
     2641 tttagatctg atccttttgc aggctgacct tctggagctg caagctcgac acaccggtta
     2701 tgccacagga gtggttatag agtctcgcct ggatcgagga agaggcccgg ttgctacggt
     2761 gattgtgcaa gaagggatat tgaaagtggg agattatttt gtagcaggaa gcagctgggg
     2821 aagggtgaag tcattattaa attatctggg aaaaaccgtt cgtgaggctt atccctctac
     2881 gcccgttgag gtggtgggtt tttccgattt accaccggcg ggaactaaat tgatggtggt
     2941 gggggaagaa aaaatagccc atctcatcgc cgagcggaga aaagagagag aaaaagaaaa
     3001 attattgaag gaatccagac cttattctat atctctggaa gaaatgcttg ctccagaaga
     3061 aggaaaaagt cgagagctca atctaatttt gaaggccgac tttcaagggt cgcttgaagc
     3121 tctggagaaa gctattgctt ctttagagga agaagaagta aaaattaata ttgtgtatca
     3181 aggagtaggc aacatcactg aaaccgatat tatgttagct tcggcttcgc agggaatagt
     3241 tattggcttt aacgttagat tgctaagtga ggcagctaaa atagccaaaa gagaaggagt
     3301 ggaggtaaga ctttatcggg tcatctatga ggtgattgat gatatagaaa aagccattaa
     3361 aggtcttata gagcctcgtc tggtggaaga gacgctgggt agagcagaag ttagagcgat
     3421 atttaaaata ccccgggtgg gagagattgc tggttcttat gtgacccagg gtaaaataga
     3481 gcgaggggct ttagcgcgaa ttttgagggg ggaagaagtc ataaaagaag gagccaaaat
     3541 aacctctctc aagagattta aagaggatgt aagagaagta actcaaggat atgagtgtgg
     3601 aataggttta gaaaactttg acgatttcca ggaaggggat atactggagg cttatgtcat
     3661 taaagaagaa gaaaaatgag agtaggagcc tgtcaaattg atatctttat taatgagagc
     3721 ttttctctta aagataaaag aagagtggtt aattctctga aagaaaggct gcggaatcgg
     3781 tttaacgttt cagtgattga aatttctact aattcccggt ggaggaaaag tagtctggga
     3841 atctcctgtg tgggaaacga tgagcggagt gtccagcttc tccttcagca agtggttcaa
     3901 tttgtagaag gagacgaccg cctggaaatc accaatttga ccatagaaat ttattgagga
     3961 ttggaaaata agcggaaagc caggtgaatc ccgatgaaag aacaaagagt agaacgggta
     4021 gcagaattaa ttaaaagaga agtgtcaatt attttgttat ctcgggtgag ggaccctcgc
     4081 ttgaagcgat ttaccatcac tcgagtgaaa gtatctcaag acctgcggca agccaaggta
     4141 tttatttatt ttcccgaagg ggataaagaa gaaaaatacc aggcactgca aaaagctacc
     4201 ggttttataa aaagtgaatt agctcttcgt ctgagaatca agtttatgcc cgaaattgtt
     4261 tttgtgatag atgatactat ggaaaaagct tttcaggtgg tagaacttct taatcagatg
     4321 gaaaggaaag aagatggaag cgaaaaagga gataattgag gccttccagc tccataatca
     4381 ttttataatc atttctcatc agaatattga tggagatgga ttggggtcta tgcttgccac
     4441 ctttttcttt ttaaaagaag aagggaaaga atcgtttatg gttcaagatg aggcaatacc
     4501 ttacttttac gagtttttgc ctggaaagga agagctgctg actctggagg agttctattc
     4561 tctgaattct tcttctgaga gagatgtggt aatagtttta gattgttccc atcccgatcg
     4621 cctgggaaga ttaaaagagc tatgcaacgg ggcctctctt cttgtgaata tagaccatca
     4681 ccccgataac gctcgttttg gagatataaa ctgggtagtc cccgactccc cttcttccac
     4741 tcttctggtt tacgagctaa taaaggaagc aggagtaaaa ataagctccg atatggcgat
     4801 taaccttctt actggttttg tgaccgatac tggtggtttt caatttgtgg atatggattc
     4861 tcgccttttg caagtggtag aagatttgat taactcaggc gcatctcttg cccagataat
     4921 gcgttatgct tttcactttc ggagattaga agctttgaag ctcttagggc gggcgcttga
     4981 ccatcttttt tatgatgaag attttcggtt ttcggtgatt tatttaacta aagaagactt
     5041 tgaagcctgc ggggcgcaag aggaagatag tgagggaata gtagattatg ggctttatat
     5101 cccgggagca gagctttctc tcttttttaa agaagtggag gatgggtctt ttagggtgag
     5161 tttgcgctct caggaagaaa gggatatttt gcctatcgct cattattttg ggggaggagg
     5221 gcatcataaa gcggcgggat tcaaaatcga aggcgatttt ccccgggtaa aagaggaagt
     5281 tttaagtgtg gtgagaaaat tcctgagcaa tccctcatcg cttactgaag aggtaaaaaa
     5341 agagagtgcc gggcggaata cttaatgttt ataagccggt aggaatgact tcctttgaaa
     5401 ttacagaacg ggtgggaaaa atcttgggag aaaaagctgg ccacactggc acccttgacc
     5461 ctttcgcccg aggggtaatg gtagtttgct ggggtaaagc cacgcgcctt gcttcttttt
     5521 tccaggctct ggataaaact taccgggctc gaatccgttt tggtatatct accgatactt
     5581 atgatgttac aggaaaaata gtaaaagtta gaaaaggcaa tttatcaaaa aaggaaggag
     5641 aggagcttct cctcaaatat cgaggagcaa taaagcagca ggtgcctcct ttttctgcca
     5701 gtaaacaccg gggcaaacct cgttatcggt atgctcgcca gggcaaaaca ctccctgtgc
     5761 aggaaaaaga agtagagatt tatgatctgg agctcatatc ctgggaagaa ggagagtggc
     5821 cagaggcaga aattttagtt cgctgctcca gtggaactta catccgcagt ctggctcacg
     5881 agtggggaga agatagcggt ttgggcgctt accttttttc tcttcgtcgg gaaaaagtag
     5941 gcgatttttc tctggagcag agtgaagaga taacagaaag ggaaatagac cggcagtttc
     6001 tcctctcaaa aagcattccc cccgatcagg gtttgtactg gattacctcg gttcacttag
     6061 aagagaaaga agcgaaaagg ataaaacatg gagggtttgt cttttctccc tggcaaaaaa
     6121 aaggatgggt taagatgtat gagggggata aatttttagg tctgggtaaa atagaagatg
     6181 atggagaatt gagaccccgg ataattttag gagaatagat atgtggcaga gaggaataaa
     6241 atctgcagag aataatattt tagctctggg cttttttgat ggagttcatc gcggtcacca
     6301 atacctctta ggcgaagctt accgcctggc tcaagaggag aaagctaaac tgcatatttt
     6361 tactttttat cctcatcctc gaaactttct ctcttcagaa gaggtcaagc acatccttac
     6421 ttatcgagag aagtattgcc tgatacagga gctatttcct cagtcccttt tgcaatttgt
     6481 gcgttttaac aagagcttcc gagaaatgac agctcaggag tttttaactc ttgtggaaca
     6541 ggaaattaga ccccggggag tggtggtggg agagaatttt cgttttggtt tccaggctca
     6601 aggcgacgct cagcttttga aaaaccatct ttcttttaga ggaatagaag taacagtggt
     6661 tccttccctt cgggaagggg gagagcttat ctccagctcc cgcattagat ggcatttaga
     6721 aagcggcgat ttactttctg ctaatcgctt attaggttat tctttctttc tcgaggggcg
     6781 agtccgacgg ggaaaaggag tgggaagaaa attgggattt cccactgcca atctttatcc
     6841 tccttcttac aagcttcttc ctgcatctgg agtgtatacc ggttacgttt ccggaaaaga
     6901 gataggagag agaaaccctt ctcttattta tgtaggccac aggcctacgg taaatttttc
     6961 tcctcgccgg ctggtggaag ttttcatccc ggaagtctct cgagagcttt atggagaaag
     7021 gctgaccata gaattccagg ctttcttgag agaagaaatg gtttttccct ctctttcgga
     7081 gctcaaaaac cagattgagc aagacttgga ggctttacgc atcactacag aacaatctgc
     7141 tatatcccct ttccaccact gcgaacacgg tgcagagaga ggagaacagg ctacctttag
     7201 ttagttgaga atcaggcctt tgctgtgctc tctataataa gaagaaaacg atgcataacc
     7261 attttatagg acttttgctg acctcatagc tcttgtcaaa catttcttta cccatcttta
     7321 atggttgtgc tgatgagcta tcatctttcc tgaagaatga atttcaaatt ctcgagtgcg
     7381 tacagtaaga gagaa
//
LOCUS       DAOPSS010000076         7187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8615_length_7187_cov_8.489624, whole genome shotgun sequence.
ACCESSION   DAOPSS010000076 DAOPSS010000000
VERSION     DAOPSS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8615_length_7187_cov_8.489624"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKN31_05550"
     CDS             <1..182
                     /locus_tag="PKN31_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99423.1"
                     /translation="SISISGKDETVIAGLHTTQELMRVTTRPFFAPLTGLCDRFLSFQ
                     KARLVVMKRTEQPAA"
     gene            complement(443..847)
                     /locus_tag="PKN31_05555"
     CDS             complement(443..847)
                     /locus_tag="PKN31_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99424.1"
                     /translation="MRFLKLVLILSLCLVLLTALAGCSPAGADLEGTPEEDSTTTPTT
                     PTTPPSIDTTACLKECLDFCAEWCQSCTVCNCEEGPVTLPGGETCLGQCFGFCEEWYD
                     ACQECEEEETCQPCQPQEEPEEGCAGGHCPLN"
     gene            complement(1045..2112)
                     /locus_tag="PKN31_05560"
     CDS             complement(1045..2112)
                     /locus_tag="PKN31_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99425.1"
                     /translation="MKKVLLVLIIILFISGSALAQDLIVEKNPEEIFIFAGDSSFYQK
                     ETWVVELQPGENIYSWEKPFSVQEEEVFIQVEGGELVSLISDSSNESTKIIIVAEEQT
                     KGKIYLIFPLLYLDLNYLYQYFWEPDKNVPAGTLLVDITNEGQEPIPESHFIISGKEF
                     IFGLKPYETKRLVVDEFQAVRAEKISRYNPLSYGDTDVHFFWDLFVSGDILFPAKIEC
                     FEGSTSGVVFLGESYTLGESPELEIKVGKSNDITIEEKIEKQDKLNQVYNREGKEVLY
                     DTQEKKVYKVANRSPERKKIEVFVPLQESYELISHSVPLSRVESNQISFVLEIEPQEE
                     VEVVLEVAGKNLTSGFAFKSW"
     gene            complement(2109..3287)
                     /locus_tag="PKN31_05565"
     CDS             complement(2109..3287)
                     /locus_tag="PKN31_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99426.1"
                     /translation="MKKYSLILAFFLSIFFGFSPISHALLSQSVSPDESWLTIYQNID
                     RAWVVEKVTLSLEEGENRFFLTGDNLLRERLYFHPLDPLTSLKTIATTSANGYQVEIE
                     AQEAGSYSFLLSFFMSGFNWKGRYMAIWDERDSLFYLYPSVFLSNHKSMTWENVHISW
                     LLGEPVFLFEETARGIADEMKLAVEEARPRVMKSEKPVAMERVSEYQVFHLPYMVDFP
                     GNSLLQVFPEMQKLSVKEVIRIEEGNEVRVLKMKNQGEPLPGGWITIFRENQSVIGSF
                     EFPGARTQEELEIPVGAVKDFRTERVEVSYKRLKADWNKDKDVVGFWSQKVIRFNLLN
                     HGESSKAVEIIEPLPQGAELTGDENWKWEDGKAYLELTVEPDSEEEIVLQYEFREDWP
                     "
     gene            complement(3521..4405)
                     /gene="hflC"
                     /locus_tag="PKN31_05570"
     CDS             complement(3521..4405)
                     /gene="hflC"
                     /locus_tag="PKN31_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOA99427.1"
                     /translation="MSRKRLTQLTMVLIIIAVVVIIRPWYILDETEQAVILQLGKPIA
                     TIKESGLHLKLPYPLQVVYKFDKRLLEYDSPPEEVITEDKKTLLIDYYARWKIVDPLL
                     FLQTVVSEARAMVRIDDIAYSELRTEVGKVELMDIVATERGQIMERVTAKTNLKAQDF
                     GVEVVDVRIKRADLPSQNEEAVFQRMQTEREQQAKQYRSEGEEEATKVQAAADREKAL
                     ILAEARKEAQIIRGEGEAEALKIYAEAYNQDPEFFAFLRTMEMYEKSFTEKDTLILTP
                     ESEILNYLKSPRQSATRD"
     gene            complement(4398..5390)
                     /gene="hflK"
                     /locus_tag="PKN31_05575"
     CDS             complement(4398..5390)
                     /gene="hflK"
                     /locus_tag="PKN31_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOA99428.1"
                     /translation="MFYDEPEGKVINLHDSKITRFFKRLGYLIIVIVIAIYFLTGIYV
                     VGPDEVGMVRTLGKFSRQVPPGLHYHLPYPFQTVVKPKVTELRRIEIGFRTLYPGPPP
                     HHQLFPEESLMLTGDENIVDCQYIVQYRISDPYAYLFLIRDAENAVRNAAEAAMREVV
                     GKKGIDEVLTVGRSDVQQEARSLTQEILDSYQSGIDVVAVQLQDVQPPQEVAAAFRDV
                     ASAREDRARFINQAQAYRNQVVPQARGEAAKILEEAQAFQATVIKEAEGEAARFLRVL
                     SEYQLNPDISRERIFLETMETVWPQVNKFIFDSQENNESVLKFLPLVTREAEDE"
     gene            5509..7185
                     /locus_tag="PKN31_05580"
     CDS             5509..7185
                     /locus_tag="PKN31_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOA99429.1"
                     /translation="MALLTGWQAVVKALCLEGIQFVYGLPSSPKDLYDALYDEPSIKA
                     IQVRHEAAGGFMAMAHALSTGEPAVCYASQGPGIANITPAMLESLAACAPVILLGAGV
                     DGHIYGKGAFQETDQISIMSPITKWAVRVPYAEKIPWTIRRAFFLATNGQPGPVYIEI
                     PPEVGRNRADIPDYIPAVRHLKTAGDYNQIKKAIELISRSKRPVIVAGGGARRSRAHD
                     ELKELADLLGMPIMTTPSGRGSIEEDHPLHFGGVGIYRNSLGIKTFAEADLLITVGSR
                     NEEFQTGAWKLFPEGAKFIQIDIESFEIGRNWLPDIPIWGDAKIVLASILEGIRDLAT
                     PKWEERREKYSEEKRAYEAQVEEECRKCNDIPIKSKRVVYEINQVFGRNTIMVHENGS
                     QDLWSYYSPYYRVLDRDGDIAPGEQTCMGAGVMGAVGVKLAHPDKKVVCVTGDGAFQM
                     YNQDVPTAVQYDAPVTWVILNNFSLGWPKVGQKELGGRYIATDFTVQPDFVQMAHAYS
                     CYGERIEKPEDIRGALERALKANESGKPAILDFIVDPDDLAEGFNLYRKL"
BASE COUNT         2036 a         1675 c         1465 g         2011 t
ORIGIN      
        1 tctccatatc catatcggga aaagatgaaa cagttattgc agggctacac accacgcaag
       61 agctaatgcg tgtcaccacc agaccatttt ttgcaccttt gactggtctt tgtgaccggt
      121 ttttatcttt ccagaaagca agattggtag tgatgaaaag gaccgaacag cctgcggcgt
      181 aagattaaaa aaaacagaga aagccaaaat aatcacgctg aaaaagaaca ctcagcgcat
      241 ctcccgaaag ggaatgctcc tgcaattcgc tcaaaaacag tcgcaatata tagctttaag
      301 catatcagta atcagcctca gaaagttaaa aagatggcaa aagtccatcg gagagattat
      361 taaaaatata ggcggcaaaa tatgcgcacc gtctcttctt ttttaatttt agcttctcct
      421 tttagaataa cttataaggt aactaattga gaggacaatg gccgcccgcg caaccttcct
      481 cgggttcttc ttgcggctgg caaggctggc aagtttcttc ctcttcgcat tcttgacagg
      541 cgtcatacca ttcctcgcaa aaaccaaaac attggccaag gcaggtttct ccaccgggca
      601 aagttactgg cccctcttcg cagttgcaaa cagtgcagct ctgacaccat tcggcacaaa
      661 agtccaggca ttctttaaga catgcggtag tatcaataga aggtggtgta gtaggtgtag
      721 taggtgtagt agtagagtct tcctcagggg tgccttctaa atctgcacct gcaggcgaac
      781 atccagcaag ggcggttagc aaaaccaggc aaaggctcag gattaaaact aattttagga
      841 acctcacaaa tttcaccttc tttttaagga ttaacttgat tttgataaaa ttgagcctcg
      901 atgacgttga actctgctca ttataccatg caagacaaca acccacaatg cagcatgcct
      961 ttagagcctc tcttcgtcct ctctcatatg ggtatcatta atcttaccag aaccagccac
     1021 ggaaagcgga ttatttgttt aatattacca gcttttaaaa gcaaaacccg aggttaaatt
     1081 tttaccagcc acttctaaaa caacctccac ctcctcctgg ggctcaatct cgagaacaaa
     1141 tgatatctgg ttactttcaa cccgacttaa aggaaccgaa tggctaatta actcataact
     1201 ctcctgaaga ggaacaaaaa cttctatttt ctttctttcc ggggaacggt tggctacttt
     1261 gtatactttt ttctcctggg tatcataaag cacctctttc ccctctctgt tataaacttg
     1321 atttaattta tcttgctttt ctattttttc ttcaatagtg atatcattgc ttttccccac
     1381 ttttatttcc agctcaggcg attctcccag ggtatagctt tcgcccaaaa atacaactcc
     1441 tgacgtagac ccttcgaagc attctatttt agcggggaaa aggatatccc cagaaacaaa
     1501 gagatcccag aaaaaatgaa catcggtatc cccataagag agaggattat aacgcgatat
     1561 tttctctgcc cgaacagcct ggaattcatc aacaacgagt cgtttagttt cataaggctt
     1621 taaaccaaaa ataaattctt tcccggaaat aataaaatgg ctttcaggaa tcggctcctg
     1681 accttcattc gttatatcca ccaataacgt tccagctgga acatttttat ccggctccca
     1741 aaaatattga tacaaatagt tcaggtcaag gtacaaaaga ggaaagatta aataaatttt
     1801 cccttttgtt tgttcttctg ccactattat tatcttggtg ctctcgttag aagaatcact
     1861 aatcaaactc actaattcac caccctcaac ttgaataaac acttcctcct cttgcaccga
     1921 aaacggtttt tcccaggaat aaatattttc cccgggttga agctcaacca cccaggtttc
     1981 cttttgataa aacgaagaat ctcctgcaaa aataaaaatt tcttccggat ttttttccac
     2041 tattaaatcc tgagccagag cactaccgga gataaaaagg attataatca ataccaacaa
     2101 tacttttttc atggccaatc ctcccgaaat tcatattgca aaacgatttc ctcttcgcta
     2161 tcaggttcaa cagtcaattc cagataagct ttcccgtcct cccatttcca gttctcgtct
     2221 ccggttaatt cggctccctg aggcaggggt tctattattt ccacagcttt acttgattca
     2281 ccgtggttta aaagattgaa acgaatcacc ttttgagacc agaaacccac cacatcttta
     2341 tctttattcc aatcagcctt caagcgttta tagctcactt ccacacgttc agtccgaaaa
     2401 tccttgaccg ctcccactgg tatttccagc tcttcctgag ttcttgcccc cggaaattcg
     2461 aagctcccta ttaccgattg attttctctg aatatagtta tccatccccc cgggagaggc
     2521 tcgccttgat ttttcatttt taaaaccctg acctcgttcc cttcttcaat tctaatgact
     2581 tccttcacgg aaagtttttg catctcgggg aaaacttgta agagactatt accgggaaag
     2641 tcaaccatat agggcaagtg aaaaacctgg tattctgaca ctctttccat tgctactggt
     2701 ttttcgcttt tcatcactcg aggccgggct tcttccacag ccagcttcat ttcatctgca
     2761 atcccccttg ccgtttcctc aaataaaaat accggctctc cgagcagcca gcttatatgc
     2821 acattctccc aggtcatgct tttatgattg gatagaaata ctgagggata aaggtaaaaa
     2881 agagagtccc tctcatccca tattgccata taccgtcctt tccaattaaa tccactcata
     2941 aaaaaggaaa ggagaaaaga ataggaaccg gcttcttgag cctcaatttc aacctgatat
     3001 ccatttgcag aagtagtagc aatggttttc aaagaagtta aaggatcgag aggatgaaaa
     3061 tagagtcttt cgcgaagaag attgtctccg gttaagaaaa agcgattctc cccctcctcg
     3121 agtgacaaag tgactttttc aaccacccag gcccggtcaa tattctgata aattgttaac
     3181 caagattcat caggactcac ggattgggaa aggagagcgt gggatattgg tgagaagcca
     3241 aaaaaaatac tgagaaaaaa cgctaatatt aaactgtatt ttttcatact tcacctccgc
     3301 aaacaactaa ttttaactct ttacttccaa agcgcaacct gtctatcaaa acatgtttaa
     3361 tgaagtattt tctcaaattt gtaatatttt taaaactccg cagaaaactt atctcttaaa
     3421 tttacagtta atttcgcggt gacagaaaga agagcgctgc aggcacgctg gaatgtattt
     3481 cccagcattg ttgtgtttag ttttttaata atttacgctt ttaatctcgc gtcgcagact
     3541 gccttgggct tttcaagtaa tttaatattt cagattcagg agtgagaatg agggtgtctt
     3601 tttcagtaaa agatttttca tacatttcca tagttcttaa aaaagcaaaa aattccgggt
     3661 cctggttata ggcttctgcg taaatcttga gagcctcggc ctccccttct ccccggatga
     3721 tttgagcttc ttttcttgct tcagctaaaa tcaaggcctt ttcccgatcg gcagcagctt
     3781 gaactttagt tgcctcttct tcaccttctg agcggtactg cttggcctgc tgttctcttt
     3841 cggtttgcat tcgctgaaaa actgcttctt cgttctggga ggggaggtca gcccgtttta
     3901 tcctgacgtc taccacttcc accccaaaat cttgagcctt gaggttggtt ttagcggtta
     3961 ccctctccat aatttgccct ctctctgtgg ctactatatc catcagctca actttgccga
     4021 cctctgtccg aagctcagaa taggcaatgt catcaattct caccatcgcc ctagcctcgc
     4081 tcaccactgt ttgcaaaaaa agcaagggat ccactatctt ccagcgagca tagtagtcaa
     4141 ttagcagagt ttttttgtct tcggtaatta cctcctcagg aggagaatcg tattccagca
     4201 gccttttatc aaatttgtaa accacctgga ggggataagg tagcttgagg tggagcccgc
     4261 tttcttttat cgtagctatt ggtttaccta actgcagaat aactgcctgt tcggtttcat
     4321 ccaggatata ccagggacgg ataatcacta caaccgctat aattatcagc accatagtaa
     4381 gctgagttaa cctttttcta ctcatcttca gcctctctcg tcactaaagg taaaaacttt
     4441 aaaacgctct cgttattttc ctggctgtcg aatataaatt tattcacttg aggccatacg
     4501 gtctccatgg tttccaggaa aatccgctct cgagagatat cggggttaag ctggtattcc
     4561 gagagaactc ggaggaaacg agcagcttct ccctcagctt ctttaataac cgtagcctga
     4621 aaagcctgag cttcctccag aattttagcc gcctctcctc tcgcctgagg aacaacctga
     4681 ttacggtagg cctgagcttg attaataaaa cgggctcgat cttcccgggc actggctaca
     4741 tctctgaaag cggcggccac ttcctgcggg ggttgaacat cttgcagctg aaccgctacc
     4801 acatcaattc cgctctggta ggagtctaaa atttcttgcg tcaaagacct ggcttcctgt
     4861 tgaacgtcag atcgccctac ggtgagcacc tcatcaatgc cttttttgcc caccacttct
     4921 cgcatagcag cttcggccgc atttcgtacc gcattttctg catcgcgaat aagaaacagg
     4981 taagcataag ggtcagaaat tcgatattga actatatact ggcagtctac aatattttca
     5041 tctccggtga gcattaagga ttcttccgga aaaagctggt ggtgaggagg aggaccgggg
     5101 tacagggtgc gaaaaccaat ttctattctc ctaagttcgg ttactttggg ttttactacg
     5161 gtttgaaaag gataaggaag atgataatga aggccggggg gtacctgccg agaaaatttc
     5221 cccagagttc ttaccatacc aacctcatca ggacctacaa cataaatccc agtcaggaaa
     5281 taaatggcta ttacaattac tattatcaag taccctaaac gcttaaaaaa gcgggtaatc
     5341 ttcgaatcat gcaaattgat aaccttgcct tcgggctcat cataaaacat aacaatttca
     5401 attgtacgct atcagatttg aatttgcaaa ttttgccggg ctctggcaaa gcccctggga
     5461 gaaatataag ctataataca ggtaaaagaa cctgcaagga ggaagaaaat ggcgttgtta
     5521 actggatggc aggcagtagt aaaagctttg tgtttagaag gtattcagtt cgtttatgga
     5581 cttccgagca gtcccaagga tttgtacgat gctctttatg acgagccttc catcaaggcg
     5641 attcaggtac gacacgaagc agcaggagga tttatggcta tggctcatgc tctctcgact
     5701 ggtgagccgg ccgtttgtta tgccagtcaa ggaccgggca ttgcaaatat aacacctgct
     5761 atgctggaat ctttagcagc atgtgccccg gttatactgc ttggagcggg tgtagatgga
     5821 catatatacg gcaagggtgc cttccaagag accgaccaga taagcattat gtcccctatt
     5881 accaagtggg ctgttcgagt tccttatgct gaaaaaatcc cctggactat tcgcagagct
     5941 tttttcctgg cgacaaatgg acagcctggc cctgtataca tagaaatacc tcccgaagtt
     6001 ggcagaaatc gagctgatat ccccgattat atacctgccg tgcggcatct caaaacagcg
     6061 ggagattaca accagattaa aaaagccatc gaactcattt cccgcagcaa acggccagta
     6121 attgttgccg gtggaggcgc acgaaggtct cgagctcacg atgagctgaa ggaattagca
     6181 gatttactgg gcatgcctat aatgactact ccttcaggac gaggcagtat agaagaagac
     6241 catcctcttc actttggagg agtcgggata tacagaaaca gtctggggat taagacattt
     6301 gctgaagcag atttgctgat caccgtgggt tctcgcaatg aagagttcca gactggtgcc
     6361 tggaagcttt ttcctgaagg ggcaaagttc atccaaattg atatcgagtc ctttgagatt
     6421 ggtcgtaatt ggcttcctga tatccccata tggggagatg ccaaaatagt gttggcttca
     6481 atcttagaag gtataagaga ccttgccact ccaaaatggg aagagcgccg ggaaaaatat
     6541 tctgaggaaa aaagggctta tgaggcccag gtagaagagg aatgcagaaa gtgtaacgat
     6601 atacccataa agagcaaacg ggtggtttat gaaatcaacc aggtcttcgg acgcaacacg
     6661 attatggttc atgaaaacgg gagtcaggac ctctggtcat actacagccc ttattatcgg
     6721 gtgttagatc gagatggaga cattgcccct ggagagcaaa cctgtatggg cgcaggtgtt
     6781 atgggtgctg taggagtaaa acttgcccac cccgataaaa aggtagtatg tgtcaccggc
     6841 gatggtgcat ttcaaatgta taatcaggat gttcccacag cagttcaata tgatgcaccg
     6901 gtcacctggg tgatattgaa caattttagc ctggggtggc ccaaggtagg gcaaaaagag
     6961 ctgggtggcc gctatattgc aacagacttc actgttcagc cagatttcgt ccagatggct
     7021 catgcctaca gctgttatgg tgaacgcata gaaaagccag aagatatccg tggtgcactg
     7081 gagcgagcac taaaagcaaa cgaatcgggt aagccggcta tcctcgattt catagtcgat
     7141 cctgacgact tagccgaggg ttttaatcta tatcgaaaac tgtaaaa
//
LOCUS       DAOPSS010000077         7163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8645_length_7163_cov_8.157569, whole genome shotgun sequence.
ACCESSION   DAOPSS010000077 DAOPSS010000000
VERSION     DAOPSS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8645_length_7163_cov_8.157569"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..632
                     /locus_tag="PKN31_05585"
     CDS             138..632
                     /locus_tag="PKN31_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOA99430.1"
                     /translation="MARDINCFFGIGNLTKEPDLRYTPQGTPVCRFSIAMNRSYSGKQ
                     GMVKDVTYITVAAWSKLAEICARYLHKGSRVAVMGELRSSSWEDREGNRRISYEIRAD
                     NVQFLSPPRGAVQEEVEEFSGLGANFEMEEAEPFVYEGGESPLGELEDIPLEKDDGEE
                     VAPF"
     gene            550..2220
                     /locus_tag="PKN31_05590"
     CDS             550..2220
                     /locus_tag="PKN31_05590"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOA99431.1"
                     /translation="MKAEKARWESWKIFPWRKMTGKRLLPFKGKVFILFLFFSLLPLF
                     SLGAQVEINWGEESFYSEAEVRVRQGRTYVNVEELFREIGGIVYYSPIMNKINLGFKG
                     TAWVLSLDKGVAVHDGREVPLDGEVFIEGNAPFVSLDLLGQLFSFASSVSSVPSPSLT
                     PSPEPSFSPLPERERDSFFILSGIRFHPYPEEMRTRVTFDFQGGVPSYSYRIDREENR
                     IAVELLNCDWRQPTSSIAVGDGRIDRIEVTKTADGLRVNIWMQNPIQVKEEKKLPGDN
                     PRIYFDLVSLVEARVEASPEVKPTTTPRASPTVKPTPTTPQEASGLSYNEVNTKVVVI
                     DPGHGGNDPGCVYNGYKEKDVVLAISQKVKAALESKGYQVFLTRSQDVYPSLNDRYRV
                     ANEHRPFVFLSIHCNASPNPQATGVEVFVGGVKPRGEGAGDVASRENQLFLKEGSAAD
                     ANRVGNILSSAYYQSSREVSAELGKIMSEKISSTTGQKNRGLKEAPLVVLDGMYYPAC
                     LIEVGFLSNAEEAKKLASSAFQEKIAQAIAQAVDDFSRNPKFKSFLGE"
     gene            2222..2851
                     /locus_tag="PKN31_05595"
     CDS             2222..2851
                     /locus_tag="PKN31_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOA99432.1"
                     /translation="MQDQIPPRKKYKEKESSWPNWFYVLVGLAIFFAVLYFGEMILRY
                     QQPPEISPSPGVTLPPEEELGKLPPSQEEEVILYFSNPDFTALVGEKRKIKKDSKWME
                     RILEELLKGPKDRSLYNPIPPSTRLNAVFLEGDIAYVDLSVEMAKNQSGGTSQELLSV
                     YAIIDTLTAFPEIRKVKILIDGEETSTLCGHIDISQPLERDERLIAKVQ"
     gene            2862..3632
                     /gene="rph"
                     /locus_tag="PKN31_05600"
     CDS             2862..3632
                     /gene="rph"
                     /locus_tag="PKN31_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOA99433.1"
                     /translation="MGNFYFKKDRATWRGRPDGRQPDELRPVEIRLHYLRYAEGSVLV
                     EAGNNRIICAASVEDRVPPFLRNSGQGWITAEYSMLPRATTTRSLRESIRGQVGGRTH
                     EIQRLVGRALRSVVDLERLGERTIMIDCDVIQADGGTRTWSITGAFIALVEALWNCVD
                     RGILSSGGIVKDYLGAISVGVVGGQELLDLAFEEDSAAQVDMNVVMTGSGDLVEVQGT
                     AEGDPFSRETLNRLLDLAQKGVGEIIVLEKKLLKRGLP"
     gene            3629..4228
                     /gene="rdgB"
                     /locus_tag="PKN31_05605"
     CDS             3629..4228
                     /gene="rdgB"
                     /locus_tag="PKN31_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOA99434.1"
                     /translation="MSGEVYLVTKNEGKLKEVKDIFAPFSISVRSVYEVCDIGEVEEK
                     GESYAHNALIKARAGFVNTGKISLGEDSGLEVDYLRGAPGIYSARFGGENASSGDKIS
                     LLLELLQGVPQEERTARFVCVVALVWEEGEEIFRGVCEGYIAEEPRGSGGFGYDPVFV
                     FPPEGKTFAELGSEFKNRHSHRFKAFRQCAEFLVREVFH"
     gene            complement(4205..4756)
                     /locus_tag="PKN31_05610"
     CDS             complement(4205..4756)
                     /locus_tag="PKN31_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOA99435.1"
                     /translation="MEVQIGKCRLIIKQGDITEEDTDAIVNAANSRLAGGGGVDGAIH
                     RAGGPKIAEECRKIGGCPTGKAVITSGGNLPASYVIHAVGPIYRGGKNNEAELLRSAY
                     ISSLELADKHNLRSVAFPSISTGAYGYPIKEASRIALQAIIDYCKSHPQTTLEEIRFV
                     LFTPQDKEVYVQSLKELMENLSD"
     gene            4844..5971
                     /locus_tag="PKN31_05615"
     CDS             4844..5971
                     /locus_tag="PKN31_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOA99436.1"
                     /translation="MNKVTLLTRIFPEGERVSEVVVEYDSEIDGSNLTPDSYAVEAKV
                     GDTTVERTIKKVYANSSFDLVCSTNRGRFVIIELDHEEAVAKTLVFSSETFLSERPEL
                     IYTVTQKVAITTPEGETIAPFTLQNTDEKHLIVDDFQAFVYRDERSGVEVPYRFFVPR
                     NVNPDKKYPLVVFLHGAGERGRDNFIQLVANKGAVVWAQPGHQALHPSFVLAPQCPRE
                     SSWTGVLSGGEPFEPNPELLALPHLIENLVSQHNIDSDRIYVTGLSMGGFGTWAITME
                     SPHLFAGAIPVCGGGEVKKVDKIKHIPIWAFHAEDDPLVPVAFSRSLVRSLAERGGRV
                     KYTEFLKDYLESQGWSAHSSWIPAYEDETVINWLFEQKRGQ"
     gene            6253..6933
                     /locus_tag="PKN31_05620"
     CDS             6253..6933
                     /locus_tag="PKN31_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014287867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOA99437.1"
                     /translation="MKKWGKFPGVMIFWGVLMMTGISGGGERIILPSPGLRGEVSVEE
                     AILKRRSVRSFLDDALSLGELSQILFSAQGITEKNQGFRAAPSAGALYPLVIYAVVGK
                     VEELTPGVYRYYPQGHAIEKVLEGDRRGELYRAALSQGAIRGAPVSLVFAAIYERTTV
                     KYGERGIRYVHIEIGHAAQNVYLQAEALGLGTVAIGAFYDEEVARVLSLPPEEAPLYI
                     MPVGKVRK"
BASE COUNT         1919 a         1380 c         1875 g         1989 t
ORIGIN      
        1 aggaggtgat aagtggattt agctcttata tctccatata attaaagaaa aaaatttctt
       61 ctctatttga gaatgaagaa taatgtgtta aactcatata cagaataatt ttcgatgaaa
      121 tagggggatg ttttctgatg gctcgagata ttaattgttt ttttggaatc gggaatttaa
      181 ccaaggagcc cgatttacgt tatacccccc agggaacacc ggtttgtcga ttcagtatcg
      241 ctatgaatcg tagttattca gggaagcagg ggatggtgaa ggatgttacc tatatcactg
      301 tggctgcctg gtccaagctg gcagagattt gtgctcgtta cctccacaag ggtagtcggg
      361 tggcagtgat gggagagtta cgttccagtt cctgggaaga tcgcgaaggt aaccgtcgta
      421 tctcctatga aattagagcg gataatgttc aatttttgag ccctcctcga ggagcggtgc
      481 aagaggaagt agaagagttt agtgggttgg gagcaaattt tgaaatggag gaggcagaac
      541 cgtttgtcta tgaaggcgga gaaagcccgc tgggagagct ggaagatatt cccctggaga
      601 aagatgacgg ggaagaggtt gctccctttt aagggaaaag tttttattct ttttttgttc
      661 ttttctcttc ttcccctttt ttctctgggg gctcaggttg agataaactg gggagaagag
      721 tctttctatt ctgaggcaga ggttagagtt cgccagggta ggacttatgt aaatgtagag
      781 gagttgtttc gggaaatagg ggggattgtt tattattctc ccattatgaa taaaataaat
      841 ttaggtttta aaggtacagc ctgggttctt tctctggata agggagtggc agtgcatgat
      901 ggaagagagg tccccttaga tggagaagtg ttcatagaag gcaacgctcc ttttgtctct
      961 ttagaccttt taggccagct tttttctttt gcttcttctg tttcttctgt cccctctcct
     1021 tctcttaccc cctctccgga gccgtctttt tctccccttc ctgagaggga aagagattct
     1081 ttctttatcc tttctggaat tcgcttccat ccttatccgg aagagatgag aaccagggtt
     1141 acttttgatt ttcaaggggg agttccctct tattcttacc gtatagaccg cgaggagaat
     1201 agaatagccg ttgagctttt gaactgtgac tggcgacaac ctacatcttc tatcgcagtg
     1261 ggtgatggga gaatagaccg tatagaggta acaaaaactg ctgatggctt aagagtcaat
     1321 atctggatgc agaatccgat acaggttaaa gaggaaaaga agttgcccgg agataatcct
     1381 cgaatctact ttgatctggt ttctctggtg gaggccagag tagaagcatc gccggaggta
     1441 aagccaacta ccactcctcg agcatcacct acagttaaac ctactcccac cactcctcag
     1501 gaagcttccg gcctttccta taacgaggtt aacactaaag tggtggttat agaccccggt
     1561 catggaggaa atgaccctgg ctgtgtgtac aatggctata aggagaaaga cgtggtgctg
     1621 gctatttctc agaaagtaaa ggcggcttta gaaagtaagg gttaccaggt ttttctgact
     1681 cgctctcaag atgtttatcc ttctttgaac gaccgatatc gggtggctaa tgaacaccga
     1741 ccttttgttt tcttgagcat ccactgcaat gcttctccca atcctcaagc tacaggtgtg
     1801 gaggtgtttg tgggaggggt gaagccccgg ggagaaggag caggagatgt ggccagccgg
     1861 gaaaatcagc tttttttgaa ggaaggaagt gcagcagacg ctaaccgggt gggcaatatc
     1921 ttatcttctg cctattacca gagcagtcgg gaagtcagtg ctgagctggg caaaataatg
     1981 tcagaaaaaa tctcttctac taccgggcag aaaaaccgag gattgaaaga agcacctctg
     2041 gtggttctgg acggtatgta ttatccagct tgtctgattg aagtgggctt tttgagcaat
     2101 gcagaagaag ccaaaaaatt agcttcttct gcttttcagg aaaaaatagc tcaagctatt
     2161 gctcaggcag tagatgattt tagtcgaaac cccaaattta agagcttttt aggagaataa
     2221 aatgcaagac caaattcctc ctcggaaaaa gtataaagaa aaagaatctt cctggccaaa
     2281 ctggttctat gttcttgtgg gtttagctat tttctttgct gttctttatt ttggagaaat
     2341 gattttgcga taccagcagc ctccagaaat ctctccctct cccggagtca ctcttccccc
     2401 ggaggaggaa ttaggaaaac taccaccttc tcaggaagaa gaggtaattc tttatttttc
     2461 taaccccgat tttactgctt tagtaggaga gaagaggaaa ataaaaaaag acagtaagtg
     2521 gatggagaga atattagaag aattgttaaa aggccccaaa gaccgttctc tgtacaatcc
     2581 catacctcct tccactcgtt taaatgcggt gtttttagaa ggagatatag cttatgtaga
     2641 cttgagtgta gagatggcta aaaatcaatc aggaggtact tctcaggagc ttttgagcgt
     2701 ttatgccatc attgacacct tgactgcttt tccagaaata aggaaagtaa aaatattaat
     2761 cgatggagag gaaacttcta ctctgtgtgg tcatatagac atttctcaac ctttagaaag
     2821 ggatgaaaga ttaattgcaa aagttcaata atttccagga attgggtaat ttttatttta
     2881 aaaaggacag ggcaacctgg aggggaagac cggatggtcg tcaacctgat gagttgaggc
     2941 cggtggagat acgcctccat tatctccgat atgccgaagg ctcggtttta gtggaagcgg
     3001 gcaacaaccg cattatttgt gcagcttctg tagaagacag ggttcctcct tttttgcgca
     3061 atagtggtca aggctggatt actgcagaat attctatgct cccccgtgcc accaccaccc
     3121 gtagtttgcg agaatctata aggggacagg tggggggaag aacccacgaa atccagagat
     3181 tggtgggcag agctctgcgc tcggtagttg atttagagag gttgggagag agaaccatca
     3241 tgatcgattg cgatgttatc caggctgatg ggggaacaag aacctggtct ataaccggtg
     3301 cttttattgc tctggttgaa gctctgtgga attgcgtgga ccggggaata ttatcttccg
     3361 gaggtatagt aaaagattat ctgggagcca tcagtgtggg agtagtggga gggcaagaac
     3421 tcctggatct tgcttttgaa gaagattctg ctgctcaggt agacatgaat gtcgtgatga
     3481 ccggcagtgg agatttggta gaggtacagg gaacagcaga aggagaccct ttttcccggg
     3541 aaacactaaa tcgcctttta gatttagccc agaaaggggt tggagaaatt attgttttag
     3601 agaaaaaact cctgaaaagg ggtctgcctt gagtggtgag gtatatttag ttacgaagaa
     3661 tgaggggaaa ctaaaggaag taaaagatat ctttgctccc ttttccatct cggtgagaag
     3721 cgtttatgaa gtttgtgata taggggaagt agaagaaaaa ggggaaagct acgctcataa
     3781 tgctctcata aaagctcggg cagggtttgt aaatacaggt aaaatctctt taggagagga
     3841 ctcggggtta gaggtggact accttcgtgg agcgccgggg atatattcgg cccgctttgg
     3901 gggggagaat gcttcttccg gggataaaat atctctcctt ttagagcttc tgcaaggagt
     3961 cccgcaagaa gagcgcaccg ctcgctttgt ttgtgtggtg gctttggtgt gggaagaggg
     4021 agaagaaata tttcgggggg tatgtgaagg ctacatcgcc gaagaacccc ggggaagtgg
     4081 gggttttggt tatgaccctg tttttgtctt cccccccgag ggaaagactt ttgccgagtt
     4141 aggctctgaa tttaaaaatc gccacagcca ccgctttaaa gccttccgtc agtgcgctga
     4201 atttttagtc agagaggttt tccattagct cttttaaact ctgaacgtag acttccttgt
     4261 cttgaggagt gaagagcaca aagcgaatct cttctaatgt agtttgagga tgacttttgc
     4321 aatagtcaat tatagcctgc aaggcaatgc gagaggcttc ttttatggga taaccgtagg
     4381 cgccggtgga tatagaggga aaagccacgg aacgcaggtt gtgtttatcg gccagttcga
     4441 gactggaaat ataggcactg cgcaggagtt cggcttcgtt atttttccca ccccggtaaa
     4501 ttggtcccac agcgtggatg acatatgaag caggcaggtt ccctccgctc gttataactg
     4561 cttttcccgt ggggcagcct cctatcttcc ggcattcttc agcaattttc gggcctcctg
     4621 cccggtgaat tgctccatct actcctcccc ctcccgcgag gcgagagttg gcagcgttga
     4681 ctatggcgtc ggtatcttct tcggttatat ctccctgttt gattatcagg cggcactttc
     4741 ctatctggac ttccattctt ccacctcctt tttcattata tcacagtatc acaggaagat
     4801 aagtgaggtg atataatgaa aaaaactcaa aggaggagaa gaaatgaaca aagttactct
     4861 gcttaccaga atttttcccg agggagaaag agtgagtgag gtagtggtgg aatatgactc
     4921 tgaaattgac ggaagcaatc ttactcctga tagctacgcg gtggaggcaa aagtgggaga
     4981 cacaacggtt gagagaacca tcaaaaaagt atacgctaac tcctctttcg atctggtatg
     5041 ttctaccaat cgaggaagat ttgtcattat agagttagat cacgaagagg cagtagctaa
     5101 aactcttgtt ttttcttcgg agactttttt atcagaaaga ccggagctaa tttatactgt
     5161 gactcagaaa gtagctatca caactcctga gggggaaacc atcgctcctt ttacccttca
     5221 aaacaccgat gaaaagcacc taatcgttga tgattttcaa gcttttgtat accgggatga
     5281 aaggtcgggg gtggaggtgc cctaccgatt ttttgtcccc cggaacgtta atccggataa
     5341 aaaatatcct ctggtagtat tcctgcacgg agcaggagaa agaggaagag acaattttat
     5401 ccagttggta gcaaacaaag gagcggtggt ctgggcacaa cccgggcatc aagcactaca
     5461 cccttctttt gtattagctc cccaatgccc ccgggagagc agctggaccg gagtgctttc
     5521 aggaggagag ccttttgagc ctaatcctga gctgcttgcc cttcctcatc tgattgaaaa
     5581 tcttgtttct caacacaata tagatagcga tagaatatac gttaccggct tatctatggg
     5641 ggggtttgga acctgggcga taacaatgga aagtcctcat ctttttgccg gagccattcc
     5701 tgtgtgtgga ggaggagagg tgaaaaaggt agataaaatt aaacatatac ccatctgggc
     5761 attccatgct gaagatgacc ccctggttcc cgttgctttt tccagaagtc tggtgaggag
     5821 tctggcagaa agagggggga gagtaaaata tactgaattt ctcaaagact atctggaatc
     5881 tcaggggtgg agtgctcact cttcctggat tcccgcttat gaggatgaaa ctgtaattaa
     5941 ttggttgttc gagcaaaaga ggggccagta gcgggttcat taaaacgatg aagcctggag
     6001 catcccctgg ccgtttaagc gggaagttcc ttttcgggat acgataataa caggtgagag
     6061 attttacttt gatattatag tatcccgaaa aaagtcaaaa aacgggaaat tgccatgcag
     6121 caagggatac tataagttct ccagtggtct tttgcttttt gggggtcatg aggggtcctc
     6181 atagtttgtt attatgtatc gcatcaggta agactcacaa tgacggttaa ttataaaaag
     6241 ggggggagaa atttgaaaaa gtggggaaaa ttccctggag taatgatatt ttggggggtg
     6301 ttaatgatga caggaataag cggaggtgga gaaaggatta tcctgccctc acccgggttg
     6361 agaggtgagg tttcggtgga agaagctatt ttgaaaagac gctcagtgcg ctcttttctc
     6421 gatgatgcac tatctctggg tgagctttct caaattctat tttctgccca ggggataaca
     6481 gaaaaaaatc agggttttcg tgctgcgcca tcagcgggag cgctttatcc tctggtgatt
     6541 tatgcagtag tagggaaagt ggaagaatta accccggggg tttaccgcta ctaccctcaa
     6601 ggacacgcca tagagaaagt gctggaaggg gatagaagag gggagcttta ccgtgctgct
     6661 ctttctcagg gggctatccg gggagctcca gtatctttgg ttttcgctgc aatttatgag
     6721 agaaccactg taaagtatgg agagcgagga attcgctatg tccacataga gataggacat
     6781 gcagcgcaaa atgtctacct gcaggctgaa gcactggggt tgggaacggt agctatcggt
     6841 gctttttatg atgaggaagt tgcccgggtg ctttctttac ctccggagga agcgcctctc
     6901 tacattatgc cggtggggaa ggtaaggaag taaaagctaa aaattttgcc gatggttttg
     6961 aaaggtggga ttttcaagtc cgtcattcct gaatgtattt atcatcccgt tatctctcgt
     7021 tcctgaatgt ctctattgcc ccttattcgt caccccggca atctttaagc cggggtccat
     7081 ggtttttaaa agtatttaga ttcccgatta aatttcggga ataatggtgc gagacacgcc
     7141 tatggttcac aatgaaagga caa
//
LOCUS       DAOPSS010000078         7120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8710_length_7120_cov_9.659306, whole genome shotgun sequence.
ACCESSION   DAOPSS010000078 DAOPSS010000000
VERSION     DAOPSS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8710_length_7120_cov_9.659306"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PKN31_05625"
     CDS             complement(<1..828)
                     /locus_tag="PKN31_05625"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99438.1"
                     /translation="MNSALNKLIFLIKEIEKKKLIYLDVVSPVRPFFALFLEKPLLYV
                     VSDREKRKKVVSQLEALQSVAGKPFLVEGVKREEEFFGVKLQSNFRQKEVVAVVEAQD
                     LHLEVEDLDYFLTHSFLLEKGKETRREEFLDLLFSLGYERQELVRERGEVAIRGEVVD
                     LFPPHLPLPVRTYWWGNQIEKMKFFRPESQRTEEEVKTLFIPPRSGEFKTVPLLSSLK
                     KEVKTIFWDGVIVEDLTLSSRQVFSGIGTQEAQDSFSLEVERIPSFWGKIDDLKDYLE
                     "
     gene            complement(837..1409)
                     /gene="lptC"
                     /locus_tag="PKN31_05630"
     CDS             complement(837..1409)
                     /gene="lptC"
                     /locus_tag="PKN31_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOA99439.1"
                     /translation="MGKRLLLIVSFCVVGFLLVFFLVSSIPQESQDKEEVEEEMEEVA
                     PSPPVVMKGTELRGWEGGKLAWLLQAQDMELNDDNTKAICWGAVRLIVYQEGEEKATI
                     QAQEAEINLDDKDFYFKGEVEVVSSSGEKIVTSELRYRDKDKVLETEAEASVHFDGNF
                     IHCRGLRSDVDFENPEFYQIIKGSFRLSAM"
     gene            complement(1409..3124)
                     /locus_tag="PKN31_05635"
     CDS             complement(1409..3124)
                     /locus_tag="PKN31_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA99440.1"
                     /translation="MNNKVVIIGGVAAGPKVAAKLRRLDPEVEITIIEKGKFLSYAGC
                     GLPYYISGEIKEYRELMETPIGVMRDPEFFENMKRIKVLNRTEAIRIDRENKRVEIKD
                     LESGEVSSLPYDYLVLATGAKVSIPPISAVDLNHPGVALSPTDLAHVYTLHGVEDAEA
                     IRWTIKEKLARRAVIIGGGLIGMEMAESLVKSGLEITILEILPEILPILDEDMGILVR
                     RYCQSEGINIRVGEGAQRLEGEGGVVKRVITDKGSYEADMVVIATGFKPNDDLARKAG
                     LEVGERGGIKVDLFLRTSDPFIFAAGDCIEIENLVCGQPAYMPMGSLANKQGRVVAMN
                     LAGRREAFRGALNSVIFKVFDYTVARSGLGVKQAQNLGYDVEYSLVPAPDRAHYFPGA
                     KQVIIKLVADKRSGRVLGAQMIGKGEVTKSIYALATLLNYQGKVEDISNLDLPYAPPY
                     ASAIDNLCLAANVLRNKLEGDMVGISSQEVEKKRKRGEDFILLDVRTPREYQNIRIPG
                     SVLIPLAELPARANELSPDKEIVTICAAGLRGYEASLILKSKGFQKVMVMDGGMATWP
                     YEIER"
     gene            complement(3153..3452)
                     /locus_tag="PKN31_05640"
     CDS             complement(3153..3452)
                     /locus_tag="PKN31_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99441.1"
                     /translation="MGTFLKVVGVVFFCLGIVAIIFSLVGMSKQKSSPSYSLPPPGEE
                     FYPFPEEEFFSSTSDFFSKLAGIGGILGGFGVLVGGAALFCLGSIYNDVKSLKNR"
     gene            complement(3469..4533)
                     /locus_tag="PKN31_05645"
     CDS             complement(3469..4533)
                     /locus_tag="PKN31_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA99442.1"
                     /translation="MDKFAFIIHAFSPQDIKDHLKFLKILPSSWVESLASRVPPFVLS
                     EVKGVKSEATGKEIGGWFIGLPMTPRVLLEYSFSFVAQQIRKCGFLAEELGAKIIGLG
                     AFTSVAGDGGITVKNGLRIAVTTGDSYTVATALQALEMASSMMEIDLGQEEIAIVGAT
                     GSIGRACALILAEEGKKVRVIGRDPEKVEKVKKEVEEIKGEEVKGFIDIEEGIKGSRA
                     LITVTSALGNLLEPAWIERGAVVCDVARPRNVAEEVAKERQDVLVIEGGVVDVPGEPD
                     FGMDFGYPPGKSYACMAETMILTLEGRFEDYTLGKEIEVERVKEIDLLAQKHGFKVSG
                     LRSFGRELNEEEIEEIKKRI"
     gene            complement(4538..4975)
                     /locus_tag="PKN31_05650"
     CDS             complement(4538..4975)
                     /locus_tag="PKN31_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOA99443.1"
                     /translation="MAKVSSSLVINRSVEEVYDKAKDIEKLATFLPDIKEVKVLSQEG
                     SVVDSFWRGEFQGREIKWKERDFWDEEGKECRFSLIEGDFKQYEGAWRFREEGEDKTR
                     VELEIEYDLGIPLVGALISKFLKKVMEDNAQAMLKALQEVVEG"
     gene            complement(4981..6255)
                     /locus_tag="PKN31_05655"
     CDS             complement(4981..6255)
                     /locus_tag="PKN31_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA99444.1"
                     /translation="MDDELREKQKIVEETIDKYVKYVSPGLARLFRFANFSTVEWRGE
                     GALVFDIYGEAYIDCIGGFGSFPVGRNHPRVVQKVKEQLDRLPLSTRTLFNKQQADLA
                     EMLAQVTPGDLQYSFFGNSGAEAVEGAIKLARFYTGRKKLVSAINSFHGKTMGALSVS
                     GRDVYKKPFEPLVPETYQVPFDDIEALEEVVDEKTAAVILEPIQGEGGINIPHPRYLS
                     RVKEICEQNGALLILDEVQTGLGRTGKMFACEHYEVAPHIMTLAKGLGGGVLPIGAFI
                     STEEIWKVFEDNPLIHSSTLGGNPLACTAGLETLKVLQEENIPAQAEEKGNYWMEELR
                     FLQEKFPDLVKEVRGIGLLIGMEFFDSDVASLLAAEMAERKVLVAYTLNNPRVIRLEP
                     PLTIKKEEIEKVTKALGESLDKVRETLKEIGG"
     gene            complement(6305..7066)
                     /locus_tag="PKN31_05660"
     CDS             complement(6305..7066)
                     /locus_tag="PKN31_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOA99445.1"
                     /translation="MDKKAFLIDMDGVLLYGDQAIPGAKEFIERLREKDYKFLILTNN
                     SRYTPSDLQHRMNDAGIEIEAQHFFSSAMATAHFVSSQKPRGRAFVLGGTGLYQALNE
                     AGYSFTDYRPDFVILGEMESYPYEKIVRACQLIMQGVPFIATNPDSSGPGEGGIVPAC
                     GAVAALIEEASGYAPYFVGKPNPLILRLALRYIDEHSESTIMVGDRMDTDVKVGLESG
                     LETILVLSGVTKREDIGKFPYRPNHILDSVADIEV"
BASE COUNT         1957 a         1930 c         1154 g         2079 t
ORIGIN      
        1 ttccagataa tctttcaaat cgtcaatctt gccccaaaaa gaaggtattc gctctacttc
       61 aagagaaaaa gaatcttgag cttcctgagt accgattccg gaaaatactt gccgggaaga
      121 aagagtcaga tcttccacta tcaccccatc ccagaaaatg gtttttactt ctttttttaa
      181 agaagaaaga aggggtacag ttttgaactc tccgcttcga ggagggataa aaagagtctt
      241 tacttcttct tcagtacgct gactttcggg acggaaaaac ttcatctttt ctatctgatt
      301 tccccaccaa taagtgcgaa ccggaagagg aagatgagga gggaaaaggt ctaccacctc
      361 tcctcttatc gccacttctc ctctttctct aactaactcc tggcgttcat aacccaggct
      421 gaaaagaaga tccaaaaact cctctcttct ggtttccttt cccttttcta aaagaaagga
      481 atgagtgaga aaataatcca gatcttccac ttccaggtga aggtcttgag cttctaccac
      541 tgcaacaact tccttttgcc gaaaattaga ttgtaacttc accccgaaga attcttcttc
      601 cctcttaacc ccctccacca aaaaaggctt ccccgctaca gattgtaaag cttctaattg
      661 agagacgacc ttttttctct tttccctgtc gcttaccaca taaagaagag gtttttctaa
      721 gaaaagggcg aaaaaaggcc gtaccgggga gacaacatcc agatatatta attttttctt
      781 ctctatttcc tttatcagga aaattaactt atttaaagca gaattcataa tacctctcac
      841 atagcactca atcgaaaact tccctttata atctgataaa attcggggtt ttcaaaatcg
      901 acatcactgc gcaaaccccg acaatgtatg aaatttccat caaaatgaac cgaggcttcc
      961 gcctccgttt ccagaacttt atctttatct cgatatcgca actccgatgt tactatcttc
     1021 tctcccgagg aagacactac ctctacttct cctttaaagt aaaagtcttt atcatccagg
     1081 tttatttccg cttcctgagc ttgaatggta gccttttctt ccccctcctg gtagacaatg
     1141 agccttaccg ccccccagca gatggcttta gtgttatcat cgtttagttc catatcctgt
     1201 gcttgaagta accatgctaa ttttcctcct tcccagcctc gaagctctgt tcccttcatc
     1261 acaaccggag gagaaggagc cacctcttcc atttcttcct ccacttcttc tttatcctgg
     1321 gattcctggg gaatagaaga gacaagaaaa aagaccagca gaaaaccaac cacacaaaaa
     1381 ctcacaatca ataataacct ttttcccatt accgttctat ttcataaggc caggtagcca
     1441 tgcccccatc catcaccata actttctgga aacccttgct cttcaaaatc agggaagctt
     1501 cataaccccg caaacctgcg gcacaaatag ttacaatttc tttatcagga gaaagttcgt
     1561 tagctctggc tggaagctca gctagaggaa taagaacact acctggtatg cgaatattct
     1621 gatattctcg aggggtgcgc acatctaaca aaatgaaatc ttctcctcgc ttcctcttct
     1681 tttctacttc ttgagaagaa atacctacca tgtctccttc cagtttattg cgcaggacat
     1741 tagctgccag gcacaaatta tctatagcag aagcataagg aggagcataa ggtaaatcca
     1801 ggttggatat atcttctacc ttcccttgat agttaagaag agtagctaaa gcatagatgc
     1861 ttttggtaac ctcacctttg cctatcatct gtgcacctaa aactctacca cttcttttat
     1921 ctgctaccag cttaataatc acctgctttg ctccgggaaa ataatgagct cggtcaggag
     1981 caggcactaa agaatactcc acatcatacc ctaaattttg ggcttgtttt actcccagac
     2041 cactccgggc tacagtataa tcaaaaacct tgaaaattac tgagtttaaa gctcctcgga
     2101 aagcttctct tcggccagct aaattcatag ccaccactct cccttgctta ttagccagag
     2161 accccatagg catataagca ggttgaccac aaactaaatt ctctatctct atacagtctc
     2221 cagcagcaaa aataaagggg tcgctggtgc gcaagaagag gtccacttta attcctcccc
     2281 gctctcccac ctctaagcca gctttgcgag ctaagtcatc attgggcttg aacccggtgg
     2341 ctatcactac catatctgct tcataacttc ctttatcagt tattactctt tttactactc
     2401 ctccttctcc ttccaggcgc tgcgctcctt ctcctacccg gatattgatt ccctcactct
     2461 ggcagtaacg acgcactaaa atccccatgt cttcgtccag aataggtaaa atttccggta
     2521 gtatttccag aatggtaatt tctaaaccac ttttaaccag gctttcggcc atctccattc
     2581 cgatcaatcc tccaccaata attaccgctc gtctggctaa tttctcttta atagtccagc
     2641 gaatggcctc agcgtcttca acaccatgca gagtataaac atgagctaaa tcggtgggag
     2701 aaagagccac cccgggatga tttaagtcca cagctgagat gggagggatg gaaaccttgg
     2761 ctccggtagc taaaaccagg taatcataag gtaaactgga tacctcaccc gattctaaat
     2821 cctttatttc tactctttta ttttctctat ctatacggat agcttcagtg cggttcagca
     2881 ccttaattct tttcatattt tcaaaaaatt ctggatcccg catcactcca atgggtgttt
     2941 ccattaactc ccgatattct tttatctctc cgcttatata gtaaggtaaa ccacagccag
     3001 cataagagag aaatttacct ttttctataa tggtaatttc tacctcggga tccagacggc
     3061 gtagctttgc tgccacttta gggcccgccg ccactcctcc tataattacc actttattgt
     3121 tcatttgtca ccttcctcgc attccttaac cctcaccggt tttttaaact tttcacatcg
     3181 ttatagatac tacccaagca aaaaagagcc gctccaccca ctaacacccc aaatcccccc
     3241 aatattcctc ctatacctgc taacttagag aaaaaatccg aggtggaaga aaaaaactct
     3301 tcttctggaa aggggtagaa ctcttccccg ggaggaggaa gtgaataaga aggggaactt
     3361 ttttgtttgc tcatccccac cagagaaaaa attatcgcca ctattcccag acaaaaaaat
     3421 acaacaccca ccactttcaa aaaagtgccc actaatatag ccctcccctc aaattctttt
     3481 tttgatctcc tctatttcct cttcgttcaa ttctcttcca aagctccgca gaccagaaac
     3541 tttaaatcca tgtttttgag ccagaaggtc aatctctttt accctctcta cctctatttc
     3601 ttttcccaga gtataatctt caaatcgtcc ctccagagtc aaaatcatag tttccgccat
     3661 gcaagcataa gattttcctg gaggatagcc aaaatccatg ccaaaatcag gctctcccgg
     3721 aacatcaacc actcctccct cgataactaa aacgtcctgt ctttcctttg ctacttcttc
     3781 tgccacgttg cgaggacgag ccacatcgca gaccaccgct cctcgctcaa tccaggctgg
     3841 ctctaacaag ttccccagag ccgaagttac ggtaatcagt gcccgagacc cttttattcc
     3901 ttcttcaata tctataaaac cttttacttc ctctcccttt atttcttcca cctctttttt
     3961 taccttttcc accttttccg ggtcccggcc aattactctt acctttttcc cttcttcagc
     4021 caatatcaaa gcgcaagctc gaccaataga accggtagct cccactatgg caatttcttc
     4081 ctggcctaag tcaatctcca tcatagaaga agccatctcc agagcctgaa gagcagtggc
     4141 cacagtatag ctgtcgccag ttgttaccgc aatacgcaaa ccattcttaa ctgtaatccc
     4201 tccatctccg gcaactgaag taaaagcccc caatccgata atttttgccc ctaactcctc
     4261 ggccagaaat ccacatttgc ggatttgctg agctacaaaa gaaaaggaat attccagaag
     4321 cacccgggga gtcatgggaa gcccgataaa ccaccctcca atctctttac ctgtggcttc
     4381 tgacttcact cctttcactt ccgacaaaac aaagggaggg actcgagaag ctaagctttc
     4441 cacccaggaa gaggggagaa tctttaaaaa cttaagatgg tcttttatat cctgggggct
     4501 gaaggcatgg atgatgaaag caaatttgtc cataccgtca cccttctact acttcttgca
     4561 aagcttttaa catagcttga gcattgtcct ccataacttt cttcaaaaac ttgctgatta
     4621 aagctcccac gagaggtatt cccaagtcgt attcaatttc cagttctacc cgggttttat
     4681 cctctccttc ttccctgaat ctccatgccc cctcatattg cttaaaatcc ccttctataa
     4741 gagaaaaacg gcattctttc ccctcttcat cccagaaatc tctttctttc cacttaattt
     4801 ctctcccctg aaattctccc ctccagaaac tatccaccac tgagccctct tgacttaata
     4861 ccttcacctc ttttatatca ggcaaaaaag tagctaactt ttctatatcc ttagctttat
     4921 cgtagacttc ttccacactc ctgtttatta ccagagaaga agaaacttta gccatttcac
     4981 ttaaccccct atttccttta atgtttctct aactttatcc aaagactctc ctaacgcttt
     5041 ggtaactttt tctatctcct cttttttaat agtaagagga ggttccagac gaatcactcg
     5101 agggttgttg agagtataag caactaaaac ttttctttct gccatttctg ctgccaaaag
     5161 ggaagcaaca tcagaatcaa aaaattccat tcctatcaaa aggcctatcc ctcgtacctc
     5221 tttcaccaaa tcaggaaact tctcctgtaa aaatcggagc tcctccatcc agtaattacc
     5281 tttttcctcc gcttgcgcag gaatattttc ttcctgaagg actttcaaag tttccagccc
     5341 cgcagtgcag gccagaggat tacctcctaa agtagagcta tggataagag gattatcctc
     5401 aaaaactttc cagatttcct ccgtgcttat aaatgctcct atgggtagca cccctcctcc
     5461 caaaccctta gccagagtca taatgtgagg agccacttca tagtgctcac aggcaaacat
     5521 cttccctgtt ctaccaaggc cagtttgcac ctcatccaga atcaaaagtg ctccattctg
     5581 ctcacagatt tctttcaccc gggaaagata gcgaggatgc ggaatattaa ttcctccttc
     5641 tccctgaatg ggctccaaaa tcaccgctgc tgttttctca tccaccacct cttccagagc
     5701 ttctatatca tcaaaaggca cctgataggt ttccggaacc agtggctcaa aaggtttttt
     5761 atatacatct cttcctgaaa cgctcaatgc ccccatagtt ttaccgtgaa agctattaat
     5821 cgccgaaacc agctttttcc ttccggtgta aaagcgagcc agctttattg ctccctctac
     5881 cgcttccgct ccactgttac caaaaaagga atattgcagg tctccggggg taacctgagc
     5941 cagcatttct gctaaatcgg cttgttgttt gttaaaaaga gtgcgggtag aaagaggcaa
     6001 tctgtctaat tgctctttta ctttctgtac cactcgagga tggtttctcc ccaccgggaa
     6061 acttccaaat cccccgatgc aatcgatata ggcttccccg tagatatcaa aaactaatgc
     6121 tccttctcct ctccactcca ccgtagaaaa attggcaaat cggaacagcc gggctaatcc
     6181 tggacttaca tacttcacat atttgtctat ggtttcttct actatttttt gcttttctct
     6241 caattcatca tccatggttt tcctccttta tctatgtttg taattttctc actcttttct
     6301 gcttttaaac ttctatatcc gccactgagt caagaatatg attgggtcgg tagggaaatt
     6361 tgcctatatc ttcgcgcttc gtgactccag agagaaccag aattgtttct aaacccgatt
     6421 ccaatcctac tttgacatca gtatccatcc ggtctcccac cattatggta ctctctgaat
     6481 gctcatctat atatctcaaa gccaggcgca aaattaaggg attaggttta cccacaaaat
     6541 aaggagcgta tccagaagct tcttctatca gagctgccac agctccacag gcaggaacga
     6601 ttcccccctc ccccgggcca gaagaatcgg gattggtggc aataaaaggt actccctgca
     6661 taatcagctg gcaagctcgc acgattttct cataaggata ggattccatc tctcctaaga
     6721 taacaaaatc gggcctgtaa tcggtaaaag aataacctgc ttcattgaga gcctggtata
     6781 atcctgttcc tcccagaaca aaagctctgc ctctgggttt ttgagaactc acaaaatgag
     6841 cggtagccat agcgctgctg aaaaaatgtt gagcctctat ctctataccg gcatcattca
     6901 tgcgatgctg aaggtcagaa ggggtatagc gagaattatt agtcagaatc aagaatttat
     6961 agtctttttc tcttaaacgt tcaataaact ctttggcgcc gggtatagcc tggtcaccat
     7021 agagtaaaac tccatccata tcaataagaa atgctttttt atccataacc tcacctcaaa
     7081 aaccacatct attataattt ctccccctct tattagcaaa
//
LOCUS       DAOPSS010000079         7090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8758_length_7090_cov_8.855295, whole genome shotgun sequence.
ACCESSION   DAOPSS010000079 DAOPSS010000000
VERSION     DAOPSS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8758_length_7090_cov_8.855295"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PKN31_05665"
     CDS             complement(<1..776)
                     /locus_tag="PKN31_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOA99446.1"
                     /translation="MSDIIDFHLETTTVWSVPERGKWYTHNPKFRGNFAPQIARNIIL
                     RYSKEGDTILDPMVGGGTTLIETKLLKRRGIGRDINPEAVQLTIQNLQFEYPGALPQT
                     VEVGDVRDLQGIEDCSIDLILTHPPYLNIIKYSNGKIPRDLSNIGDVDKFCQELDLGI
                     KEFFRVLKEDAYCAILVGDTRKAQHYVPLSYYVLELFLKNGFALKEDIIKVQHHCQST
                     PYWKSLIEKYNFLMIMHEHMFVFRKPVKGENLKRIRYSMG"
     gene            complement(749..1690)
                     /locus_tag="PKN31_05670"
     CDS             complement(749..1690)
                     /locus_tag="PKN31_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOA99447.1"
                     /translation="MYSSLYLDFFKLESFDAIVDIFHDTLIDTNRGYQFFVDWQKVKQ
                     NVEKYRVELHILNSLIRCPDFDSTLCYLVTNYPEVLPVIPLLLAVRDLQIKVVKDFMD
                     ADSDTVTYDFRKRKLSQKELEELIIFFERTGLKDFFQNFSCQSIQDYIAGVEVGLDTH
                     ARKNRSGKAMELVVDPIIQDIVKQRKLPFQIESQKYFRYLSDHYGISVSSSLLNRKAD
                     FLFIGDKGEIINIEVNFFSGTGSKPQEIVDSYINRQNELHGNGFDFIWITDGLGWKGQ
                     KNQIRKGLEMIDYPLNLHFVRKGLLERILCQISSTSI"
     gene            complement(1693..2631)
                     /locus_tag="PKN31_05675"
     CDS             complement(1693..2631)
                     /locus_tag="PKN31_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOA99448.1"
                     /translation="MKVIKERGQPKPFLKWAGGKTQLLNVLATKLPESILSHLYIERY
                     VEPFVGSGAMFFFLKRHFKVNESYLIDSNPELILAFRVVQKDPETLIQALQDLEKTYF
                     CLSEKKRSEFFYHIRNQYNVQRYHLDYHRYNKNWITRASWLIFLNKTCYNGLFRLNRK
                     GEFNVPFGRYKKPVICDEETLQESSRALQDTEIICSDFSLSASYIEKGTLVYFDPPYR
                     PLNSTSNFTSYSREGFSEADQKRLAQFFREMDKRGAYLIQSNSDPKNEDPEDNFFDDL
                     YKGFVIERVPAKRFINCDATRRGEINELIIRNYGAS"
     gene            complement(2798..3769)
                     /gene="pdxA"
                     /locus_tag="PKN31_05680"
     CDS             complement(2798..3769)
                     /gene="pdxA"
                     /locus_tag="PKN31_05680"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013489749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOA99449.1"
                     /translation="MKKPSLIITMGDPSGVGPEIIVKSIRRWDIEAIPIVVGDRKILR
                     KAEEILGVKILWSDFTSPAGNGPFLYDLNNVDLASFSWGEISPSGGKASYEYIEKAVE
                     LMGEGRGEGVVTAPINKEAIHKAGIPFIGHTEIFQELTHSEEALTMFQVKSLRVFFFT
                     RHLPLKIAIEQVKKDKIAQFIPRMHYYLQRLGLSHPRIAVAALNPHGGERGLLGEEET
                     KEIIPALELVRSGGIEISGPYPADSIFWRAFQGEFDAVLSLYHDQGHIATKCLDFYKT
                     VSVTLGLPFIRTSPDHGTAMDIAGKGIANEESMLEACRHAAQYAVYY"
     gene            complement(3766..4917)
                     /locus_tag="PKN31_05685"
     CDS             complement(3766..4917)
                     /locus_tag="PKN31_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOA99450.1"
                     /translation="MVDIPERIANLPRSGIRELMEIALRDPEVIRLEMGEPHFPTPHL
                     ILEKLQDFYWQGEIKYTPTVGIPSLREKIAHHLKKEKGWEVTPEEVIILPGSLFGTVT
                     IFSSLLEPQDEVLVPDPGFTNHFSQVELCGGKMKRYHLREENGFLPDREEIESLITSR
                     TKMILINSPSNPVGVVFPEETLRQLAQLAEEYGLLLISDEAYEKYVYEGEHRGMFPFI
                     NKDNLISIFSFSKTYALTGWRVGYMVAPLYLVKHLAKVEEYLIACTSHLSQKAAEVAL
                     YLPPEEVEKMVDYYRGNRDKAVSLLEKGGFRFFKPQGGYYLWIDIREFGLPSLEFCKT
                     LVEESKVALAPGDAFGETGEGFARLSICRKREDVEEGVKRLVEFRRKRR"
     gene            complement(4930..5583)
                     /gene="fsa"
                     /locus_tag="PKN31_05690"
     CDS             complement(4930..5583)
                     /gene="fsa"
                     /locus_tag="PKN31_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOA99451.1"
                     /translation="MKFFIDTADTGEIKEALKLGVLDGVTTNPTLVSKTGRGFREVIM
                     EICSLVPGPVSAEVVSLQAEGMIKEGRELSSWAPNIVVKVPITTEGLKAIKALSQEGV
                     SVNTTLIFNPLQALMAAKAGATYVSPFVGRLDDISSDGMRLVADILQIFRNYDITTQV
                     IVASIRHPMHVVEAARLGAHVATIPFPVISKLIKHPLTDVGIDQFLADWEKVPEKPF"
     gene            complement(5585..5869)
                     /locus_tag="PKN31_05695"
     CDS             complement(5585..5869)
                     /locus_tag="PKN31_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99452.1"
                     /translation="MFLPGKGLCITLKTEKYNTIAIPWRQVISFFYKEKTATEYKNTI
                     LTKTIENTAGYSLSRIEVLVNVKCGWPRYGRLIKAREKLIILVNQQKREA"
     gene            5937..6965
                     /locus_tag="PKN31_05700"
     CDS             5937..6965
                     /locus_tag="PKN31_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOA99453.1"
                     /translation="MGSVKDLIVLSSPHEEKPGRGKFIFSDRYSVFDWGEMPDHIPLK
                     GASLCLIGAYFFEQLERAGFLTHYQGIEEEGKNKRLFELKNPANEMVVSLVRIIQPPE
                     IEGKYDYNIYQGIRGNFLIPFEFIYRNSLPAGSSLRKRLERGEVKLKDYGWDRIPAPE
                     ERLSPPVVDVSTKLEEQDRYLSWQEVEETGILQKEEIEEIRRTVLAIDGLITKEVERI
                     NLKNEDGKIELAFDKDRNLMVVDVVGTPDESRYTFRGIHLSKEIAREFYRKTSWFEEV
                     EKAKLQNPVGWRGLVKASPPPLPSSLVQLISWVYQRIAMDLTERNWFSDVPSLEEIGE
                     RISQELLI"
     gene            7015..7090
                     /locus_tag="PKN31_05705"
     tRNA            7015..7090
                     /locus_tag="PKN31_05705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7048..7050,aa:Arg,seq:cct)
BASE COUNT         2097 a         1507 c         1398 g         2088 t
ORIGIN      
        1 ttccccatgc tatagcgtat cctctttagg ttttcacctt taaccggttt cctaaataca
       61 aacatatgtt catgcataat cataagaaag ttatattttt ctattagact tttccagtat
      121 ggtgttgact gacaatgatg ctgaactttg attatatctt ctttaagggc aaatccattt
      181 ttcaagaata gttccaacac gtagtaagaa agaggaacat agtgttgagc ctttctagta
      241 tcacccacga gaatagcaca ataagcatcc tcttttaata cccgaaagaa ttcttttatc
      301 cccaagtcta attcctggca aaatttatcc acatctccaa tattagacag gtcacggggt
      361 atctttccat tcgaatattt aataatgtta agataaggag ggtgggtaag aatcaggtca
      421 atggagcaat cttcaatccc ttgaaggtca cgaacgtcac ccacttcgac ggtttgagga
      481 agcgcacctg gatattcaaa ttgaagattc tgaatggtga gctgaacagc ttcgggattg
      541 atatcccgac ctattcctcg ccgtttcaat aacttggttt caataagagt tgtgcctcca
      601 cccaccatag ggtcaagaat tgtatcccct tccttagagt agcgaagaat aatattacga
      661 gctatttggg gagcaaagtt ccctcgaaat tttggattat gagtatacca ctttcccctt
      721 tcgggcaccg accaaaccgt agtggtttct aaatggaagt cgatgatatc tgacacaata
      781 tgcgctccag aagtcctttt ctaacaaaat gtaaattgag aggataatcg atcatttcta
      841 acccctttcg aatctgattt ttttggccct tccaacccaa accatcggtg atccagatga
      901 agtcaaagcc gttaccatgt aattcattct gtcggttaat ataagaatcc acgatttcct
      961 gaggtttaga tccagttcca gaaaagaagt tcacctcaat attgataatt tcccctttgt
     1021 ccccgatgaa tagaaaatca gcttttcgat tgagaagaga agatgaaaca gaaataccgt
     1081 agtgatctga aagatagcga aagtattttt gactttctat ttgaaatggt aattttcgct
     1141 gcttcactat gtcctgaatt atcggatcca caacaagctc catcgcctta ccactgcggt
     1201 ttttgcgagc atgcgtatct aagcccactt ctactccggc aatataatct tgaatactct
     1261 gacacgagaa attttgaaag aagtctttga gccccgttct ttcaaagaag ataatcagct
     1321 cttctaactc tttttgtgaa agctttcttt tccgaaaatc gtatgtcact gtgtctgaat
     1381 cggcatccat gaagtctttg accaccttta tctgtaaatc acgaacagca agaagtagag
     1441 gaataacggg gagtacctca gggtaattgg ttaccagata acaaagcgtg gagtcaaaat
     1501 caggacatcg gataagagaa tttaaaatgt ggagttcaac cctatatttt tctacgtttt
     1561 gtttaacttt ttgccaatcg acaaaaaact ggtaccctcg gtttgtgtca ataagagtat
     1621 catgaaaaat atccacaata gcatcaaaag attccagctt aaaaaaatcc aggtaaagag
     1681 aggaatacat attcaacttg ctccatagtt acgaattatc agttcgttga tttctccccg
     1741 acgggtggca tcacagttaa taaatctttt ggccggcact ctctcgatca caaatccttt
     1801 ataaaggtca tcaaaaaaat tatcttcggg atcttcgttt ttagggtcag aattactttg
     1861 tatgaggtag gctcctcgtt tatccatctc tcgaaaaaac tgagccaatc gtttttgatc
     1921 tgcttcagaa aatccctctc tggaatatga ggtaaagtta gaagtagaat taagaggtct
     1981 ataaggaggg tcaaaataaa ccaatgtccc cttttctata tatgaagccg aaagagaaaa
     2041 gtcactacaa atgatttccg tatcctggag agcacgactc gattcttgca aagtttcttc
     2101 gtcacaaatg acaggttttt tgtagcggcc aaagggcacg ttaaattctc cctttctatt
     2161 taaacggaat aatccattat agcaagtctt gttgagaaaa attagccagg aagctcgtgt
     2221 aatccaattt ttattgtacc gatggtaatc taaatgatac ctctggacat tgtactgatt
     2281 tcgaatatgg taaaaaaatt cactcctttt tttctcagac aagcaaaagt aggttttctc
     2341 aagatcctga agtgcttgaa tgagggtttc aggatctttt tgtactactc taaaagccag
     2401 aataagctcg ggattagaat cgattagata cgattcattg actttaaaat gtcttttaag
     2461 aaagaaaaac atagctccac ttcccacaaa aggttcaaca tatctctcga tataaagatg
     2521 tgagagaatg gactcaggaa gctttgtagc cagaacatta agaagctggg tttttcctcc
     2581 tgcccatttt aaaaaaggtt tcggctgtcc tctttctttg atgactttca tacttctttc
     2641 tccagggttt cagcccatta atcttataag tcttggcttt gatagtttaa ttaaggtaaa
     2701 attattatac cacacaacat aataaacgct tagctaagtt aaagattatg tctttatccc
     2761 cctaaacaga aaaagatagg gagttcgggc tttcaattta ataatatacg gcatattggg
     2821 cagcatgtcg gcaagcttct aacatgcttt cttcattagc tattcccttt cctgcaatat
     2881 ccatagctgt tccgtggtcg ggagaggtgc gaataaaggg caatcctaaa gtcacgctca
     2941 ccgttttata aaaatctaaa cacttagtgg caatatgtcc ctggtcgtga taaagagata
     3001 agacggcatc aaattctcct tgaaaagctc gccagaagat agaatctgcc ggatagggcc
     3061 cggagatttc tatccctcca cttcgcacca gctccagagc gggaataatc tcttttgttt
     3121 cttcctctcc caaaagccct ctttctcctc catgggggtt caaagcagct accgctatgc
     3181 ggggatgaga taggcccaac ctttgaagat aataatgcat gcgagggata aattgggcaa
     3241 ttttatcttt tttgacttgc tctattgcta ttttgagagg aagatggcgg gtaaagaaaa
     3301 aaactcgcaa gctctttact tgaaacatag ttaaggcttc ctcgctgtgg gttaactctt
     3361 gaaaaatttc agtgtggcca ataaaaggaa taccggcttt gtggattgct tctttgttta
     3421 taggagcagt aaccactccc tctccccttc cctcccccat caactctacg gctttttcta
     3481 tatattcata gctcgctttc cccccggagg gagaaatttc tccccaggaa aaagaagcca
     3541 gatctacatt gttcaggtcg taaagaaaag ggccatttcc cgcaggtgag gtaaagtcgc
     3601 tccagagtat ttttactccc agtatttctt cggcttttct gagtattttc ctatctccta
     3661 ctactatggg aatagcttct atatcccacc tccttatact ttttactata atttccgggc
     3721 ccacccctga gggatctccc atggtgataa ttaaagaagg ttttttcatc ttctcttcct
     3781 tctgaattct accagtcttt ttactccctc ttccacgtct tctctcttgc ggcagataga
     3841 gagacgggca aacccctctc ctgtttctcc gaaggcatcg ccaggagcca gagctacttt
     3901 actctcctct accagagttt tacagaactc taaagacggc aaaccaaact cccggatatc
     3961 tatccacaga taatatcccc cctgaggctt gaaaaagcga aaccctccct tttctaaaag
     4021 gctcaccgct ttatcccggt taccccggta ataatctacc atcttttcta cttcttccgg
     4081 aggtaaatat agagcaactt ctgctgcttt ttgagaaaga tgagaggtac aggcgataag
     4141 atactcttcc actttagcta agtgtttaac caggtaaaga ggagcaacca tataccctac
     4201 tctccagcca gtcagggcat aagttttaga aaaagagaaa atgcttatca aattgtcttt
     4261 atttatgaaa ggaaacattc cccggtgctc tccctcataa acatatttct cataagcttc
     4321 atcagaaata agcaaaagcc cgtattcctc tgccagttga gctaattggc gaagagtttc
     4381 ctcaggaaac actaccccca cagggttaga aggggaattg atgagtatca ttttagtgcg
     4441 agaggtaatc aaactttcta tttcttccct atcgggtagg aaaccatttt cctcccggag
     4501 gtgatatctc ttcatttttc ctccacacag ctctacctgg gaaaaatggt tggtaaaacc
     4561 aggatcaggc actaaaactt catcttgagg ctccaaaaga gaggaaaaaa tcgttaccgt
     4621 tccaaacaaa gaaccaggaa gaattatcac ctcttctggt gtcacttccc atcctttttc
     4681 ttttttcaag tgatgggcaa ttttttctcg caggctgggt atacccaccg tgggagtata
     4741 tttgatttct ccctgccagt aaaaatcctg gagtttttcc aagatgaggt ggggagtggg
     4801 gaaatgaggt tctcccatct ctaaacggat aacttccggg tcgcggaggg ctatctccat
     4861 tagctctctt attcccgagc gaggtaaatt agctattctt tcagggatat ctaccatttt
     4921 aggatctcct caaaaaggtt tttcgggaac tttttcccaa tcggctaaaa attgatcaat
     4981 tccaacatcg gtgagaggat gcttgattag cttagatatg acaggaaaag gaatggtggc
     5041 tacgtgagct cccagccgtg ctgcctccac tacatgcatg gggtggcgaa tggaagccac
     5101 aattacctga gtagtgatat cgtaattacg aaaaatctgt aagatatcag ccaccagacg
     5161 catgccatcg cttgatatgt catctaatct tcccacaaaa ggactgacat aggtggctcc
     5221 tgctttggca gccatgagtg cttgcagagg attaaaaatc aaggtggtgt ttacgctcac
     5281 tccttcttga gaaagagctt tgatagcttt caatccttcc gtagttatag ggacttttac
     5341 cactatatta ggtgcccagc tggatagctc tcttccctct tttatcattc cttctgcttg
     5401 caggcttacc acttcagcag aaaccgggcc gggaaccaga ctacaaatct ccataatcac
     5461 ttccctaaaa cctcttcccg ttttagaaac taaagtagga ttagtagtca ctccatctaa
     5521 aactcccagc ttcagagctt ctttgatttc tcctgtatcg gcagtatcaa taaaaaattt
     5581 cacctcaggc ctcccttttc tgttgattta ccagtattat aagcttctcc ctggccttta
     5641 tcaatctacc atagcgcggc caaccacatt tcacattgac aagaacttct attcttgata
     5701 aagaataacc tgcagtgttc tctattgtct tcgtgaggat cgtattttta tattccgtgg
     5761 cggtcttctc tttatagaaa aacgagatta cctgccgcca cgggatagca atggtattgt
     5821 atttttctgt tttcaaggta atgcagaggc ctttacccgg aaggaacatc ttaacaaacc
     5881 cctcagggtg ctataatcta tttaacttgt tatctcaaaa gagaggagct cataatatgg
     5941 gaagcgtaaa agacttaata gttctttctt ctccacatga ggaaaaaccg gggagaggga
     6001 aatttatttt ttctgatcgg tattcagtat tcgattgggg ggagatgccc gaccatatcc
     6061 ctctaaaagg tgcttcctta tgtttaatag gagcctactt ttttgaacaa ctagagagag
     6121 ccggattctt aactcattac cagggaatag aagaagaggg aaaaaacaag aggctatttg
     6181 agttaaaaaa tcctgctaat gaaatggtag tttctttagt gaggattatc cagcctcctg
     6241 aaatagaagg gaaatacgac tataacatct accagggaat aagggggaat tttttaattc
     6301 cttttgaatt catatatcgt aattctctac ctgcaggttc tagcttacgc aaaagattag
     6361 aaagagggga agtaaaatta aaagattacg gatgggatag gattcctgct ccagaggaaa
     6421 gattgtcacc acccgttgta gacgtttcta ccaaattaga agagcaagac cgttatctaa
     6481 gctggcaaga agtagaagag acaggcatat tacaaaagga agaaatagaa gaaattcgtc
     6541 gaacagttct tgctatagat ggcttgataa ctaaagaagt agaaaggata aacctgaaaa
     6601 acgaggatgg aaaaatagaa ctggctttcg ataaagatag aaacttaatg gtggtagatg
     6661 tggtagggac tcctgatgag agccgttata ccttccgggg gatacatctt tctaaagaga
     6721 tcgccagaga gttttatcgg aaaacttcct ggtttgaaga agtagaaaaa gctaagcttc
     6781 agaatccagt gggctggaga ggtttggtga aagcaagtcc tccacctcta ccctcttctc
     6841 ttgtgcaact gataagctgg gtgtatcagc gaatagctat ggatcttaca gaaagaaatt
     6901 ggttctcaga tgtgccttct ttagaagaaa ttggagaaag aattagtcag gagcttctta
     6961 tttgacataa gaaaagagag aaagtaaaat aatcaaggga ataaacattg atgtgcgccc
     7021 gtagctcaaa ggacagagcg gcggtctcct aaaccgcagg ctgagggttc gaatcccgcc
     7081 gggcgcacca
//
LOCUS       DAOPSS010000080         7081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8780_length_7081_cov_10.229149, whole genome shotgun sequence.
ACCESSION   DAOPSS010000080 DAOPSS010000000
VERSION     DAOPSS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8780_length_7081_cov_10.229149"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PKN31_05710"
     CDS             <1..1070
                     /locus_tag="PKN31_05710"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOA99454.1"
                     /translation="IQWVIEVLTQEETRVLESVDEIEAVGHRVLHGGEKLTGSVLVDD
                     EVLEIIRECIDLGPLHNPANILGIEACRELMPDVPQVATFDTAFHGTMPEHAYIYAIP
                     YEYYEKYRIRRYGFHGTSHQYVSERAAKILDKDPRDLKLITCHMGSGVSFAAVKGGKS
                     IDTSMGLTPLEGLVMGTRSGDLDPAIPLFLMRKEGLSAEEMDEILNKKSGVLGVSGIS
                     SDTRDIEAAAPFNHRAQLALDLMIYRAKKYIGSYYAILGGLDGLVFTAGIGENSSYIR
                     KSVCQGLEHLGILIDEERNKVNRKEAFISSDQSRVAVMVIPTNEELIIARNTRALARK
                     RGENDAALHRGSEKKCRKDFP"
     gene            1039..1557
                     /locus_tag="PKN31_05715"
     CDS             1039..1557
                     /locus_tag="PKN31_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOA99455.1"
                     /translation="MKKSVGRTFHDIEEENFPSLSWEGKKLPFAQPVRVDAILTNCGS
                     EILVKGVVDTALVMSCSRCLEPSIQEIKAVFYLEYRNLSRIHRLSDIEAEASESDEVG
                     YFWEEDNYIEVTREVEEVIFINLPMKPLCSPDCRGLCPVCGANRNKEKCFCEEAIDPR
                     LAVLKNWAKENL"
     gene            1679..1858
                     /gene="rpmF"
                     /locus_tag="PKN31_05720"
     CDS             1679..1858
                     /gene="rpmF"
                     /locus_tag="PKN31_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOA99456.1"
                     /translation="MANLTNKTSRCRRNKRRTHWVVKAPQLVDCPRCHQPKLPHRVCL
                     ACGYYKGRQVVKVED"
     gene            1871..2857
                     /gene="plsX"
                     /locus_tag="PKN31_05725"
     CDS             1871..2857
                     /gene="plsX"
                     /locus_tag="PKN31_05725"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOA99457.1"
                     /translation="MRIALDGWGGDFAPEEIIKGAKEAYSSDYTLVLVGPRQELLSLY
                     SQWGMDEEHFPIENASQIVGMGESPTEALKKKPDSTIAKGIDLLAQGKVDAFISAGNS
                     GAVMTTAWMKLKRIVQIERPAIAALIPNPNSCTVLLDVGANVDCKPKHLLHFAVMGAE
                     YAKIVLNLLHPRVGLLNIGEEEGKGNELVRSTYQLFYSQSDFLHFDFAGNVEGHNIVD
                     GKVDVVVCDGFTGNALLKFGEGLIAFISSHLPQQIGNPQEEEKLKEFWRKLDRSEYGG
                     APLLGVEGVCLICHGSSKARDIKSAIRGAQELIKRGIADKIRERLSQLNLKS"
     gene            2864..3811
                     /gene="fabK"
                     /locus_tag="PKN31_05730"
     CDS             2864..3811
                     /gene="fabK"
                     /locus_tag="PKN31_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HOA99458.1"
                     /translation="MMRGRVTEILGIKYPILQGGMAWVSTAPLVAAVSEAGGLGIIGA
                     GGMTGEKLREEIKRTKSLTGQPFGVNLMLLSTYIKEQIEVVKEEKVPIVTTGAGNPGE
                     LVEEFAREGILTIPVVASVALAKRLLRRGIKLIIAEGMEAGGHIGETTTMCLVPQIVD
                     AVSPLPVIAAGGIADGRGMAACFALGAEGVQMGTRFVCTEECEVHHLYKEAIVKAQDR
                     STIVTGTTTGHPVRCLRNRLTRQFEELEARRAPVEEIEALGSGRLREAVCGNVEEGSV
                     MSGQIAGLIKDIKPVQQVIEDIIREFKETVKKLPQAVED"
     gene            3813..4754
                     /gene="fabD"
                     /locus_tag="PKN31_05735"
     CDS             3813..4754
                     /gene="fabD"
                     /locus_tag="PKN31_05735"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOA99459.1"
                     /translation="MGWVVLFPGQGAQKVGMTKAWWDNYRQETEEIMGKASEICGVDL
                     LRLTLEGPEEELNLTFNAQPAILATSLLTWQLWQKEFSLAVDAVAGHSLGEYSALCAA
                     ESIDFEEAIFLVRKRGELMQEAVPPGEGAMVAVLGLSQEKVENVVGEFFSQGKVEVAN
                     INSSSEIVLSLSRSLLPQVVDKLEKEGSKKVVELKVSAPFHSSFMKEAAEKFSSFLEE
                     IEIKPPRYRYVSNVNAQYVSRPEEIRDLLVRQMFSPVRWKEVMDTLYRCESHHIWEMG
                     PGTVLSKLFSREYPDVEVKNVNSWNRGRKEDEEERKR"
     gene            4751..5497
                     /gene="fabG"
                     /locus_tag="PKN31_05740"
     CDS             4751..5497
                     /gene="fabG"
                     /locus_tag="PKN31_05740"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOA99460.1"
                     /translation="MSLENQVAMVTGGRRGIGFTIAQILGKNGARVAINDVASQEEIE
                     KAVEELKEQGIEARGYIADVTKKEEIKTVVQDIINTWGQIDILVNNAGITRDALLVRM
                     KDEDWKAVLEVGVQGVYYCTKEVLRYMMKKRYGRIINISSVVGVMGNAGQTNYSTAKA
                     AILGFTKSLAREVASLGITVNAVAPGFINTEMTKKLPEEVREVWLQQVPLRRWGEPEE
                     VAQVVAFLASRAAGYITGQTIHVNGGLVMI"
     gene            5525..5758
                     /locus_tag="PKN31_05745"
     CDS             5525..5758
                     /locus_tag="PKN31_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOA99461.1"
                     /translation="MDIFSKVKEIVVDQLGVDGEDVAPDASFIDDLGADSLDIVELIM
                     AFEEEFDIEIPDEDAEKITTVGEAVEYIESKVG"
     gene            5771..6478
                     /gene="rnc"
                     /locus_tag="PKN31_05750"
     CDS             5771..6478
                     /gene="rnc"
                     /locus_tag="PKN31_05750"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOA99462.1"
                     /translation="MSSSDIDFSVLEERIGYVFKDEEVLRTALRHKSALEGRDGSCND
                     KLEWLGDAVVGFFVADYLFHHYDKPRSWLSLAKSKLVSEECLAYLARKIELARFIELG
                     KGEERSGGREKDSILASSLEALAGAVFVDSSSYEETRKVLELIFCEAGDISSLNLSVN
                     YKGLLQRWCLQYYNCLPRYETLSQGNQDFYRVEVKIGENSIAWGEGKNKKRAEQEAAR
                     KALEVLSSGDQFPNKGQ"
     gene            6478..>7081
                     /locus_tag="PKN31_05755"
     CDS             6478..>7081
                     /locus_tag="PKN31_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA99463.1"
                     /translation="MYLKKIIIQGFKSFAHLTTIDLSPGLNVVVGPNGSGKSNLIDAL
                     RWVWGDTARELRVTQNREVIFNGSKTVKPLGMAEIEVWWGDRSGVNLKVARRIFASGE
                     SEYFFCDEKIRWREWKENLEKEGLGIERLSAGIVGAGDLQALLYETPLARLQWLETVS
                     GAGEWKKKLYQISLRSEKIESRRRLLEERLKELGLQVEKWK"
BASE COUNT         2105 a         1157 c         1919 g         1900 t
ORIGIN      
        1 ctatacagtg ggtgatagaa gttttgactc aagaagaaac tcgagtttta gaaagcgttg
       61 atgaaattga ggcagtaggt catagagtac tgcacggagg agagaagctc accggctcgg
      121 tgttagttga tgatgaggtt ttagagatta tcagggaatg tattgattta ggtcccttgc
      181 ataatcctgc caatatttta gggatagaag cctgtcggga gttaatgccc gatgtcccac
      241 aggtggcaac ttttgatact gcttttcacg ggactatgcc cgaacatgct tatatttatg
      301 ctattcctta tgagtattat gaaaaatatc gtatcaggag atacggcttt catggtactt
      361 ctcatcaata cgtatcagag cgggcagcca aaatactgga taaagaccct cgtgatttaa
      421 aattaattac ttgtcatatg ggtagcgggg taagttttgc ggcagtaaaa gggggtaaat
      481 ccatagatac ttctatggga cttactcctt tagaagggct ggtgatggga acgcgctctg
      541 gagatttaga cccggccata cccctatttt taatgagaaa agaggggcta tcggctgagg
      601 aaatggatga gatattgaat aaaaaaagtg gggtgctggg tgtttcagga attagtagtg
      661 atactcggga tattgaagct gctgctcctt ttaatcatag agctcaactg gctctggatt
      721 tgatgattta tcgggctaaa aaatatatcg gctcttatta tgctatttta ggaggactgg
      781 atggtttagt ttttactgct ggtataggag agaattcttc ttacattaga aaatctgttt
      841 gtcagggatt ggaacattta ggtatactaa tagacgaaga gcggaataaa gtgaacagaa
      901 aagaggcttt tataagtagc gatcagtccc gggtagcagt aatggttatt cctaccaacg
      961 aagagctgat aattgctcga aatacccggg ctctagcaag gaaaagaggt gagaacgatg
     1021 cggctttaca tcggggaagt gaaaaaaagt gtaggaagga ctttccatga tattgaagaa
     1081 gagaatttcc cttctctgag ctgggaaggg aaaaagctac cttttgcgca accagtaagg
     1141 gtggatgcta tccttacgaa ctgtggaagt gaaattctgg taaaaggagt agtggatact
     1201 gcactcgtaa tgtcctgctc ccgctgtttg gaaccctcaa tacaggaaat aaaagctgtt
     1261 ttttacttag agtaccggaa cttgagtcgt atccatcgtc tctctgatat agaagcagaa
     1321 gccagtgaat ccgatgaagt ggggtatttc tgggaagaag ataattatat agaagtaaca
     1381 cgggaagtag aagaagttat ttttataaat ctacccatga aacctttgtg ctctcctgat
     1441 tgtcgagggt tatgtccagt ttgtggggca aatcggaata aagaaaagtg tttctgtgaa
     1501 gaagcgatag atcctcgatt ggcggtattg aaaaattggg ctaaggaaaa cttataactt
     1561 tcggagataa cacactttta aaaggctaga attctatttt catttgcaat aggtgagaaa
     1621 ttattttata ctatcctaca cttcaggatg gaaagcaaaa gaggaggtat tatgagatat
     1681 ggctaatttg actaataaga cttctcgttg tcgacgtaat aaaagaagaa ctcactgggt
     1741 ggtgaaagct ccccaattgg tagattgtcc tcgctgtcac cagccaaagt tacctcatcg
     1801 ggtttgtctg gcttgtggtt actataaagg cagacaagtg gtgaaagtcg aagactaaga
     1861 gaggctggat atgaggattg ctctggacgg atggggagga gacttcgctc ccgaggaaat
     1921 aataaaagga gcgaaggagg cttattcttc tgattatacc ctggtattgg taggcccccg
     1981 gcaggaactc ctctctcttt attctcaatg gggaatggac gaagaacatt ttcccattga
     2041 gaatgcttct cagatagtag gtatggggga gtcgcctact gaagctctta agaaaaagcc
     2101 cgattctacc atagctaaag gtattgattt actagctcag ggtaaagttg atgcctttat
     2161 atcagcaggt aatagcgggg cggttatgac tactgcctgg atgaagttaa aaaggattgt
     2221 tcaaatagag cgtccagcca ttgccgctct tatacctaat cctaactcct gtaccgttct
     2281 tctggatgta ggagctaatg tggattgtaa gcccaaacac cttcttcatt ttgcagttat
     2341 gggtgcagaa tatgccaaga tagttttgaa tctacttcat ccccgggtgg ggttattaaa
     2401 tattggagaa gaagaaggta aggggaacga gctcgtgaga tccacttacc agctctttta
     2461 ttctcagagt gattttttgc actttgattt tgccggtaac gtagaaggcc acaatatagt
     2521 agatggaaaa gtagacgtgg tggtatgtga tggttttact ggcaatgccc ttttgaagtt
     2581 tggagaaggg ttgattgctt ttatttcctc tcaccttcct caacaaatag gcaaccctca
     2641 agaagaagag aagttgaaag aattctggcg taaattggat agaagtgaat atggaggagc
     2701 acctcttttg ggagtagagg gagtatgcct aatctgtcac ggtagctcaa aagcgagaga
     2761 tataaagagt gccattagag gagcccagga gttgataaaa agaggaatag cagataaaat
     2821 tcgtgagcgt ttgtctcagc ttaatttgaa aagttagagg tggattatgc gaggaagagt
     2881 cacagaaata ttgggtatca agtatcctat tctccaggga ggaatggcct gggtatctac
     2941 tgcgcctttg gtggcggcag tttcggaagc tggagggtta ggtatcattg gagcaggggg
     3001 aatgacggga gagaagctaa gagaggagat caaaagaact aaatctctta ccggacagcc
     3061 ttttggagtg aacttgatgc ttttatctac ttatataaaa gagcaaattg aagtggttaa
     3121 agaggaaaaa gttccaatag ttactaccgg tgcaggaaat ccgggagagc tggtggagga
     3181 atttgcccgg gaaggtattt taaccattcc tgtagtggca tctgttgctc tggctaaaag
     3241 gctattgcgt cggggaataa aattaattat tgctgagggg atggaagcag gtggtcatat
     3301 tggagagaca accaccatgt gtctggttcc ccagatagta gatgctgtca gtccacttcc
     3361 cgtgattgct gccggaggaa tagccgatgg aagaggcatg gcggcctgtt ttgctttagg
     3421 agcagaagga gtacagatgg gcactcgttt tgtctgcact gaagagtgtg aagtgcatca
     3481 tctttataag gaagcaattg tgaaagcgca agaccgctct acaatagtta ctggcaccac
     3541 taccggtcac ccggtacgtt gccttcgtaa tcgtctgact cgccagttcg aggaattgga
     3601 agctcgaaga gcacctgttg aagaaataga agctctgggc tctggtagac tgagagaggc
     3661 ggtttgtggt aatgtagagg aaggatcagt aatgtcagga caaattgccg gtcttataaa
     3721 agatattaaa cctgttcagc aggttattga agatataatc agagaattta aagaaacagt
     3781 taaaaaactg cctcaggcgg tggaggatta aagtgggctg ggtggttctt tttcctggac
     3841 aaggggcaca aaaggtgggc atgactaaag cttggtggga taattatcgc caggaaaccg
     3901 aggaaattat ggggaaagcg agtgaaattt gtggggtaga tttgttgcgt ttgaccctgg
     3961 aaggaccgga ggaagagcta aacctcactt ttaatgccca accagctatt ttagctacca
     4021 gcttgctcac ctggcagctc tggcagaaag agttttctct ggcagtggat gcggtagcag
     4081 gacacagttt aggagaatac tctgcccttt gtgcagcaga gagtatagac tttgaagagg
     4141 caatttttct ggtgcgaaaa aggggggagc ttatgcaaga agccgtcccc ccgggagagg
     4201 gagctatggt ggcagtttta ggactgtctc aagaaaaggt agaaaatgta gtgggagagt
     4261 ttttctctca ggggaaagta gaagtagcca atattaattc ctcttctgaa atagttcttt
     4321 ctctttctcg ttctctttta cctcaagtgg tggataaact tgaaaaagag gggagcaaga
     4381 aagtggtaga gttaaaagtc agtgctcctt ttcactccag ttttatgaaa gaagcagcag
     4441 agaagttttc ttcctttttg gaggaaatag aaattaaacc tccccgttac cgctatgtga
     4501 gcaatgtaaa tgctcaatat gtttcccgtc ccgaagaaat cagggattta ctggtcaggc
     4561 aaatgttttc tccggtaagg tggaaggaag ttatggacac tctttatcgt tgtgaatctc
     4621 accacatctg ggagatgggt cccgggacag tgttgagcaa gcttttttct cgagaatatc
     4681 ccgatgtaga agtaaaaaat gtaaattcct ggaatcgggg gagaaaagaa gatgaggagg
     4741 aaagaaaaag atgagccttg aaaatcaagt agccatggta actggaggta gacgaggtat
     4801 tggttttacc attgctcaaa ttttagggaa aaatggtgcc cgggtagcta taaatgatgt
     4861 ggcatctcag gaggagatag aaaaagcagt agaggaatta aaagagcaag gcatagaagc
     4921 ccggggatac atcgcggatg ttacaaaaaa agaagagatt aaaaccgtgg tacaggatat
     4981 aataaacacc tgggggcaga ttgatatatt ggtgaacaat gccgggatta caagagacgc
     5041 acttctcgtg cggatgaaag atgaggattg gaaagcagta cttgaggtag gggtgcaggg
     5101 agtttactat tgcaccaaag aagtcttgcg gtatatgatg aaaaagagat acgggaggat
     5161 aatcaatatt tcttcggtag taggagtaat gggtaacgcc ggccaaacta actactctac
     5221 cgcgaaagca gctattcttg gctttaccaa atctttagcc cgggaggtgg cgtccttagg
     5281 tataacggtt aacgctgtag ctcctggttt tataaacacg gaaatgacta agaaactgcc
     5341 ggaggaggtg agagaagtct ggcttcagca ggtgcctctc cgtcgctggg gagagcctga
     5401 agaggtggct caagtggtcg cttttctggc ttctcgagct gctggatata ttaccggtca
     5461 aaccatccac gtcaatggcg ggttggtaat gatttaaaaa ctacttcagg taggaggtgt
     5521 aactatggat attttttcta aggttaagga aatagtagta gatcagttag gagtagatgg
     5581 agaagacgtt gctcccgatg cttcctttat cgatgatttg ggggctgatt ctcttgatat
     5641 cgtggagcta atcatggctt ttgaagagga atttgatatt gaaataccag atgaagatgc
     5701 agaaaaaata accacagtgg gagaagcagt agaatatatc gagtctaaag taggataatt
     5761 gtattgcccc gtgtctagtt cggacattga cttttcagtg ctggaggaaa gaattggata
     5821 tgtctttaaa gacgaagaag ttctcagaac tgctctgcgc cacaaatcag cgctggaagg
     5881 aagagatggc agttgcaacg ataagttaga atggttggga gatgcggtag tcggtttttt
     5941 tgttgccgac tacctctttc accattacga taaaccccgg agctggttgt ccttagctaa
     6001 atcaaaattg gtaagtgaag agtgtttagc ttatttagcc cggaagattg aattggcaag
     6061 gtttatcgaa ttaggtaaag gagaagaaag aagtggaggg cgagaaaaag attccatact
     6121 ggccagttcc ttagaagcgc tggctggagc agtttttgtt gactcctctt cttatgagga
     6181 gaccagaaaa gttctggaat taattttttg tgaggcagga gatattagtt ctcttaatct
     6241 ctcagttaac tataaggggc tgcttcagcg gtggtgcttg caatattata attgtctccc
     6301 tcgttacgaa actttaagtc aggggaatca ggacttttat cgggtagagg ttaagattgg
     6361 agaaaatagt atagcctggg gagagggtaa gaataagaaa agagcggagc aggaagcagc
     6421 caggaaagct ttagaagttt tgagcagcgg tgatcaattt cccaataaag gtcaataatg
     6481 tatttaaaga aaataatcat tcaaggcttc aaatcttttg cccatcttac caccattgat
     6541 ctttctccag gtttaaatgt ggttgtgggt cctaatggtt cgggtaaaag taacctcatt
     6601 gatgctctgc gctgggtgtg gggagatacg gcgcgagagc tcagggttac tcaaaaccgg
     6661 gaagtcatat ttaacggctc aaaaacagtt aaaccactgg gaatggctga aattgaagtg
     6721 tggtggggag accggtcagg ggtaaatcta aaggtggcaa gaagaatatt tgcctctgga
     6781 gagtcagaat atttcttctg tgatgaaaaa atccgctgga gagaatggaa agagaattta
     6841 gaaaaagaag gcctgggaat agagcgttta agtgctggta ttgtaggggc aggagatctg
     6901 caagctctgc tttatgagac acctctcgcg cggttgcagt ggttagagac ggtaagcggt
     6961 gctggagagt ggaagaaaaa gctataccag atttctctcc gttcggaaaa aatagagagt
     7021 agaagaagac ttctggagga gcgattgaaa gaactgggcc ttcaagtgga aaaatggaaa
     7081 t
//
LOCUS       DAOPSS010000081         7057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8826_length_7057_cov_8.662525, whole genome shotgun sequence.
ACCESSION   DAOPSS010000081 DAOPSS010000000
VERSION     DAOPSS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8826_length_7057_cov_8.662525"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PKN31_05760"
     CDS             <1..644
                     /locus_tag="PKN31_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99464.1"
                     /translation="NGFALRLSQGFPITFLPPEVGEIGNGFVAYFLPGKGYSFFKRPA
                     GMLPEDMRNLIGVVPFAFLIALLFILIFAFILSRTPFGQHTYAIGGNIDAARRAGIPV
                     DRHLIKVYMISSFFASLSGVLATLIFSIGAPTQFASSLELFAVAAVVIGGASLMGGKG
                     SMLGTFLGVLVLSVLEIGFLMSGILPFYRYVAVGVTLIIAVLIDQSFPELVYD"
     gene            659..1741
                     /locus_tag="PKN31_05765"
     CDS             659..1741
                     /locus_tag="PKN31_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99465.1"
                     /translation="MPTPEINQENKKWLYFLGRNWTILFLLGEAIVFSWMGTGFFSIK
                     NFQNILVASTTILLLAAGETFVIITGGIDLSVGFVRGLISVISAIIMRDLYAAGYSPY
                     VAILWGIVVGIGIGLLPGLVNGILVAKFRVPPFIATFGMYGIAYGVSEILCNSVPIAG
                     LPALAGFIGNGYLLYYLPGQSFTWFSPPQGIAREEIRSLIPLFPNVFMLSLLAILIFA
                     FILSRTPFGQHTYAIGGNIDAARRAGIPVDRHLIKVYMISSFFAALGGVMYTLRFVTG
                     RADAGAARMLDSIAAVVIGGASLYGGTGNMIGSLLGTLIIGVLETGMVNLGLPTHNKY
                     VYVGVILILAVLIDQFFPEFVEKGGI"
     gene            1741..2511
                     /locus_tag="PKN31_05770"
     CDS             1741..2511
                     /locus_tag="PKN31_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA99466.1"
                     /translation="MTDKNTPLLEVKDIVKRFGGLVAVNRVSMEVYPGEVVGLLGDNG
                     AGKSTLIKVVSGVYQPDEGEIYFEGEKVKINNPMDAIKLGIETLYQDLALAENLNVYS
                     NIFLGREKVKNLLGAIPVLDHEYMMEESRKVLSHLGIEIPSLKNRIKNLSGGQRQSVA
                     ISRSVYWNAKLLILDEPTAALGVTEQKKVLDLVRTLSSQGVAVIIISHQLYDVFQVAH
                     RLVVLRRGEKVGERLTKETNPDEIVGLMVGAELVEGSK"
     gene            complement(2515..2673)
                     /locus_tag="PKN31_05775"
     CDS             complement(2515..2673)
                     /locus_tag="PKN31_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOA99467.1"
                     /translation="MERYVCLICGYVYDPEEGDPDFGIEPGTPFSQLPEDWVCPVCGA
                     SKDQFEKE"
     gene            complement(2752..3645)
                     /gene="garR"
                     /locus_tag="PKN31_05780"
     CDS             complement(2752..3645)
                     /gene="garR"
                     /locus_tag="PKN31_05780"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HOA99468.1"
                     /translation="MTTKPVIGFIGLGIMGKPMSYNLLRAGYPLVVYNRTKAKVEEMV
                     KEGAQAAKNPKEVGEKSEVVITMLPDSPEVEEVILGEKGVLEGVERGKIVIDMSSIDP
                     LSTRKIGEALSLKEVEMLDAPVSGGEEGAIKGELSIMVGGKKEIFDKCFPIFQVLGKT
                     ITYMGELGSGEVTKLVNQIIVALNLAALSEAFTLGAKAGLKAETMYQAIRDGMAGSKV
                     MDMKVPRFQERDFTPGFKIDLHLKDLKNALNTALRMKVSLPFTGVVEQVMVALSGEGE
                     GEKDHGAIIKFWEELSQVEVK"
     gene            complement(3659..4585)
                     /locus_tag="PKN31_05785"
     CDS             complement(3659..4585)
                     /locus_tag="PKN31_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOA99469.1"
                     /translation="MAFSIGIDLGGTTLAGVLVDEELNILAEEKIPVDREKRVEKVIG
                     DMELLIRNFKKKGKGDCLGVGLALPGLLDWERGGIVFSENLNWRNVPVIPLLKEKLSL
                     PFFMEHDVRSGAVAEVYFGAARPFQDVLYVSVGTGIAGSLIWKRNIIRGAHEASAELG
                     HIVVEPNGPLCRCGNIGCLEALASGSAMERDAFHLSGKHVKGEEIFERAQEGVSPYQE
                     VVKRASGFLGLGLFNLVQIFDPEVIILGGGVAEAGEWWLEMIREGYENRFFWTPIIPP
                     LLLGQFRGRASVMGAAGLPILQKRGEYSQSIY"
     gene            5394..6032
                     /locus_tag="PKN31_05790"
     CDS             5394..6032
                     /locus_tag="PKN31_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA99470.1"
                     /translation="MKIAVVCASGRAGKLIVEEAMSRGHEVTAVVRDPDKAPQATKVI
                     VKDLFDLTFDDIKDCEVIIDAFGTWTPDTLSQHSTSLKHLCDILSGKPNRLLVVGGAG
                     SLYVDPEHTIRLMDTKDFPDAFKPVASSMAKALDELRKRNDVRWTYISPAADFRVDGP
                     RTGKYTLGGEELIVNSKGESVISYADYAVAIIDEAEKGNHIGQRISVVGEYD"
     gene            complement(6041..6490)
                     /gene="mscL"
                     /locus_tag="PKN31_05795"
     CDS             complement(6041..6490)
                     /gene="mscL"
                     /locus_tag="PKN31_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOA99471.1"
                     /translation="MPKLVKEFKEFASRGSIIDLAVGIAIGSAFGVLVQSLVSDLIMP
                     PIGILLGEIDFSNLFVVLKPGNPPGPYLTLASAKEAGAVTVNYGLFINHLVSFLIIAF
                     TLFLVVKTISQFQHKEEAPLPATKECPYCHTIIPIQAIRCPNCTSQL"
     gene            complement(6795..6971)
                     /locus_tag="PKN31_05800"
     CDS             complement(6795..6971)
                     /locus_tag="PKN31_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99472.1"
                     /translation="MKKPELASEKGKIKEAKDPAGWYMGHELLSPPLSFRLHPYLQEI
                     NRYYFKARDRFIIK"
BASE COUNT         2028 a         1572 c         1364 g         2093 t
ORIGIN      
        1 ccaatggttt cgctctgcgc ctttctcagg gttttcccat tacctttctt ccccctgagg
       61 taggtgaaat tggcaatggt tttgtagctt actttcttcc tggaaagggt tacagctttt
      121 tcaaaagacc ggcgggaatg ctacctgaag atatgcgtaa cctcataggc gttgttcctt
      181 tcgcctttct cattgcccta ttgtttattc tgatttttgc ctttattctc tctcgtaccc
      241 ctttcggaca gcacacctat gccatagggg gaaatatcga tgctgctcgc cgagctggta
      301 tccctgtgga ccgtcatctt ataaaagtgt atatgatttc ttcctttttt gcctctttat
      361 ccggagttct ggctactctt attttttcta ttggagctcc tacccagttt gcttcaagtc
      421 tggagctttt tgccgtcgct gcggtagtaa ttggaggagc cagtctaatg gggggaaaag
      481 gttccatgtt gggaaccttt ctgggagtat tggtgctatc ggtcttagaa attggctttc
      541 ttatgagcgg aattttacct ttttaccgct atgtagcagt aggagtaact ttgatcatcg
      601 ctgtactgat cgatcagagc ttccccgaac tggtttatga ttaaaggaga aaaaaataat
      661 gccgactcct gagattaacc aagaaaataa aaaatggctt tatttcttag ggaggaactg
      721 gactattctt ttcctcttag gagaagcaat agtttttagc tggatgggaa cagggttttt
      781 tagtattaaa aactttcaaa atatcctggt agcttccacc actattcttc ttttagcggc
      841 gggagaaact tttgtcatca taacaggtgg tatcgatctt tctgtgggat ttgtgcgagg
      901 attaatttcc gtaatttcgg ctattatcat gcgagacctt tacgctgcag gttattcccc
      961 ttatgtagcc attctctggg gaatagtggt aggaatagga ataggtctct tacccgggct
     1021 ggtaaacggt atcctggtag ccaagttccg agttcctcct tttattgcta cttttggaat
     1081 gtacggtata gcttatggtg tatcagaaat tctttgcaat agtgttccta ttgccggtct
     1141 tcctgcctta gctggtttca taggcaatgg ttatttatta tattatcttc cggggcagag
     1201 ttttacctgg tttagccctc cccagggtat tgctcgagaa gaaataagaa gtcttattcc
     1261 tctgtttcct aacgttttta tgctttctct cctggctatt ctgatttttg cctttattct
     1321 ctctcgtacc cctttcggac agcacaccta tgccataggg ggaaatatcg atgctgctcg
     1381 ccgagctggt atccctgtgg accgtcatct tataaaagtg tatatgattt cttccttttt
     1441 tgctgcccta ggaggagtga tgtacacctt acgctttgtt accgggagag cagacgccgg
     1501 agctgctcgt atgttagatt ccattgctgc agtagtaatt gggggagcca gtttatatgg
     1561 aggaaccgga aacatgatag gctctttact tggaactcta ataattggag ttttagaaac
     1621 gggaatggta aatttaggtt tacccaccca taataaatat gtctatgtag gggtaatttt
     1681 gattttagcg gttcttatag atcaattttt tcctgaattt gtagagaaag gaggaatata
     1741 atgacggata aaaacactcc tctcctggag gtaaaagata ttgtgaagcg atttggaggg
     1801 ctagtagcag ttaaccgggt gagtatggaa gtttatccag gagaggtggt tggcctttta
     1861 ggagataacg gagcagggaa atctacttta atcaaagtgg tttctggagt ttaccagcca
     1921 gacgagggag aaatctattt tgagggagaa aaagtaaaga tcaataaccc catggatgcc
     1981 ataaagctgg gaatcgaaac cctatatcaa gatttagctc tggccgaaaa cctcaatgtt
     2041 tattccaaca tttttttggg aagggaaaag gtgaaaaatc tgctgggagc gattccagtg
     2101 ttagaccatg agtatatgat ggaagaatcc cgaaaagtct taagtcacct ggggattgaa
     2161 attccttctc tgaaaaaccg aatcaaaaac ctttccggag gacaacgtca atcagtggct
     2221 atctctcgct cggtctactg gaacgccaag cttctcattc tggatgaacc tactgctgct
     2281 ctgggagtaa ccgaacagaa aaaggtactg gatctggtgc gaactttgag ttctcaagga
     2341 gtagcggtaa tcataatcag tcaccaactc tacgatgtat ttcaagtagc acatcgccta
     2401 gtagttttgc gacgggggga gaaagtaggg gagcgcctga ccaaagaaac caaccctgat
     2461 gaaatagtag gacttatggt aggcgccgaa ttagtagaag ggagcaagta aaaatcactc
     2521 tttttcaaat tgatctttac ttgctccaca aacaggacaa acccagtctt ctggcaactg
     2581 agaaaaagga gtacctggtt ctattccaaa atcaggatct ccctcttcag ggtcataaac
     2641 atatccgcag attaaacaaa cgtacctctc catttttttg ctcctctcca aataatttga
     2701 tggagaaatg atagaagact ataaccggtt agtcaagaat cattcaccaa attattttac
     2761 ttctacctga ctcaattctt cccaaaactt tattatcgct ccgtggtctt tctctccctc
     2821 tccttccccg gagagagcca ccatcacttg ttccaccact ccagtaaaag gaagagaaac
     2881 tttcatcctc agggcggtat ttagagcatt ttttaaatct ttgaggtgga ggtcaatttt
     2941 aaaccctgga gtaaaatctc tttcctgaaa ccggggaact ttcatatcca tgactttact
     3001 tcctgccatt ccatctctta tggcctgata catagtctct gctttgagcc ccgctttggc
     3061 ccccagggta aaagcctcag aaagagcagc taaatttaga gccacaatta tctgattaac
     3121 taatttagtc acttccccac ttcctaactc tcccatatag gtgatagttt tccctaaaac
     3181 ctggaaaata gggaagcatt tatcaaatat ttctttctta cctcccacca tgatggaaag
     3241 ctctcctttg attgctcctt cttcacctcc actaactggg gcatctagca tttctacctc
     3301 tttaagagaa agagcctctc ctatttttcg agtagagaga gggtcgatag agctcatatc
     3361 aattactatt ttccctctct ctactccctc taatacccct ttctctccta aaatcacttc
     3421 ttctacttcg ggagaatcgg gaagcatggt aatcactacc tcacttttct ctcccacttc
     3481 cttgggattc tttgctgctt gtgctccctc tttcaccatc tcttctacct tggccttagt
     3541 ccgattataa accactaaag ggtagccagc tcgcaagagg ttgtagctca taggttttcc
     3601 cataattccc aagccaataa atcctattac cggtttcgtt gtcaatgcaa tccccctctc
     3661 aataaattga ctgactgtat tctccccttt tttgaagaat gggcaacccc gccgctccca
     3721 taacacttgc tctacctcga aattgtccta aaagcagtgg agggataata ggtgtccaga
     3781 aaaaccgatt ttcatatccc tctctaatca tctctaacca ccactccccc gcttctgcta
     3841 ctcctccccc taagataata acttctggat caaatatttg caccagatta aataatccca
     3901 ggcctaaaaa gccactcgct cttttaacca cctcttgata gggagaaacc ccctcttgcg
     3961 cccgctcaaa aatttcctct cctttcacat gttttccgct caagtggaaa gcatctctct
     4021 ccatagcact acctgaggcc aatgcttcca agcatcctat attcccgcat cggcacagag
     4081 gaccattagg ctcaaccact atatgtccca actcagcaga agcttcatga gcacctcgga
     4141 ttatatttcg tttccaaatc aggctcccgg caattcctgt tcctacactt acataaagaa
     4201 catcctgaaa aggacgagca gcaccaaaat atacctctgc cacagctccg ctacgcacat
     4261 cgtgctccat aaaaaaagga agggatagtt tttcttttag aagagggatg accggcacgt
     4321 tacgccagtt caggttctca gaaaaaacta tacctcccct ttcccagtct aaaaggccag
     4381 gaagagcaag gcctactccc aaacaatcac ctttcccctt ttttttgaaa ttccggataa
     4441 ggagctccat atcccctata actttctcta ccctcttttc tctgtctaca ggaatttttt
     4501 cttcagcaag aatattcagt tcctcatcca caaggactcc agcaagagta gttcctccca
     4561 aatcaattcc aatagaaaaa gccaaagcac atatccccct ttcttttagc tttttaaaag
     4621 ctcttaatca ttgtggaata aagatgacgg aatttcaagg cttttcatcg aggaagttgc
     4681 aagtctttgt aatcattata cagttttaaa ctaaaagcaa ccaattcaga cataattaaa
     4741 aaattttttc aacacactcc ctggaaatag tctatggaaa tgtcgcccaa aggagatgtt
     4801 tttgcttcca taaaatgtca cctccataga tcgagagtta aaacacacgc ttccataagt
     4861 actcaccatc gcatactaat ctgtaagcta tagagtctct aagatttacg atctttctta
     4921 taaaagacag gaaaggatgg tgaaaagtgc tctctccagg actcgcgggt accaaaattt
     4981 ctttagatca ggagcttcct tttttggtgg catcttttct ccaagtatta gctcataaac
     5041 aagtactact atgctgaaca ttaactccta atcgggaact atggaaaaag cattactact
     5101 acaaaaaaac gtcattccaa atattctcaa aatacaatac ttttgcttac tccgacgaat
     5161 atcagaaatt ttccccggag tgttttccac ctcaccaaaa gataatctgc atgttccatt
     5221 tttgatgata tttaccagta aaggctcaac aaaactggat accacacctc tttttacagt
     5281 aattctctga gaactatttt gataatttta caaaagcact ataatattag tgaaaataat
     5341 ccttgcagag agcataccca aatacctcat atttctaaaa ggaggagtga actatgaaaa
     5401 ttgcagttgt gtgtgcatcg ggaagagcag gcaagttaat agtagaggag gcaatgtcga
     5461 ggggtcatga ggttacagcg gttgtccgtg atcctgataa ggcgcctcaa gccacaaaag
     5521 tcattgtgaa ggatttgttc gacctgacct ttgacgatat caaggattgt gaggtaatca
     5581 ttgatgcatt tggtacgtgg acacccgata ctctctctca gcacagcact tctcttaaac
     5641 acctctgtga tatactcagc ggcaagccga atcgtttact tgttgttggt ggcgcaggaa
     5701 gtttatatgt tgaccctgaa cataccattc gactcatgga taccaaagat tttcccgatg
     5761 cattcaagcc agttgcttca agtatggcca aagcactcga cgagctccgc aaaagaaatg
     5821 atgtgagatg gacttatatt agtccggcag ctgatttccg agtagatggt cctcgcacag
     5881 gtaaatacac ccttggtggt gaagagttaa ttgtaaattc aaaaggcgaa agcgtcatca
     5941 gctatgccga ctatgcagta gccataattg atgaagccga aaaaggcaat catattggcc
     6001 agcgtatttc tgtggttggg gaatatgatt gaccatattc tcatagctga gaagtacaat
     6061 taggacaacg aatggcctga ataggaatga tagtatgaca ataaggacat tctttagtag
     6121 caggaagagg ggcttcctct ttgtgttgga attgactaat tgttttcact actaaaaata
     6181 aggtaaaggc aattatcaga aaagaaacca gatggttgat gaataaccca tagttaacgg
     6241 ttacggctcc agcctcttta gcgctggcaa gagtcaggta aggacccgga gggttacctg
     6301 gttttaacac cacaaaaaga ttggaaaaat ctatctctcc taaaaggata cctataggtg
     6361 gcataatcaa atcggaaacc aaagattgaa ctaacacccc aaaagcgctt cctatagcaa
     6421 tacccactgc taagtcaata atacttcccc ggctagcaaa ttctttaaac tctttcacta
     6481 atttaggcat agagtcatcc ctcctccttt ttattataca gcttagagag tgcaaaaaaa
     6541 agtataactt attaagaaat tagaatgatt atcctgagaa aaaaatagga ttgtctacgg
     6601 tataagatta taagaaagag actaaaaata taaaagaata aaaaactaca agaaagaaaa
     6661 ggcgtcttcc tcaatgtaag agaagcaccc cttacaatct tcccaatatt aaaagtaaaa
     6721 actaaaacaa tcacggcgga gcaagaagca ctccagcata ggggaggaaa atattacata
     6781 tgacctctga ttaactattt aattataaat ctatctctcg ctttaaaata ataacgattt
     6841 atttcctgca aataaggatg aaggcgaaaa gataaaggtg gggagagaag ctcatgtccc
     6901 atataccacc cagctggatc tttcgcctct tttattttac ctttctcact tgctagttca
     6961 ggttttttca tcttctctct tttctccttt ctcgccaaag ctcttcctca tctgggtatc
     7021 agcctgcaga ttctggagag aataataatc aaaaacc
//
LOCUS       DAOPSS010000082         7055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8829_length_7055_cov_7.327571, whole genome shotgun sequence.
ACCESSION   DAOPSS010000082 DAOPSS010000000
VERSION     DAOPSS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_7055_cov_7.327571"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKN31_05805"
     CDS             <1..269
                     /locus_tag="PKN31_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOA99473.1"
                     /translation="TLPLLSGVLTIATLLSILWTFNDFNVIYILTTGGPGTSTDILIT
                     YIYKYAFQYLRFGPAAAMAVITFAILLTVSIIYARNYFRGGEVL"
     gene            271..1101
                     /locus_tag="PKN31_05810"
     CDS             271..1101
                     /locus_tag="PKN31_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOA99474.1"
                     /translation="MKKKNFSYYLVYPAIIFILLVLLFPFFVMISTALKPLEEVYTTP
                     PHWIPKNPTLANFSDVWFKYPLGSYFKNSLIVAVGTTIINTILCIPAAYAVARLRFRA
                     KSFIMYLFLIVQMFSPIIVVISLFKIIARLGLLDNLLSLIFTNGVFTLAFAIWLLSGY
                     FSSVPKEIEEAALIDGCSRIQSITRVLLPVAMPGVITTVIYTFITAWNEFMFALTFIT
                     SMDKRPLTLGLYNFVGRWTVQWQYLMAAAILALIPVVILFMFIEKDLVKGLAGGAVKG
                     "
     gene            1103..2110
                     /locus_tag="PKN31_05815"
     CDS             1103..2110
                     /locus_tag="PKN31_05815"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA99475.1"
                     /translation="METFNFQWHITNRCNLRCRHCYQENFQDSEGGEGYLLLVAHRLM
                     EELEKRDLYTTINITGGEPLLVGEELFSLLHYLNSHPRVEELMIITNGLLLEKNLLFR
                     LEECQKLTTLKISLEGASSFTNDAIRGRGVFEKVVDKIKLAQEISFLDLVLMFTLSKR
                     NVKELSSMLALAQNLGVEGVIVERFIPQGRGREMKEAVLSREEWRSFLEELVNVCELE
                     CTPYELLPYKAFWIKGGKEGEVLGALCNLREALCLMPEGTIFPCRRFPYPLGNILKDG
                     LGKAIDSPFLETLKDRKYLKGRCSYCQVEGCYGCRALAYALFDDPYYEDEQCFWKETM
                     S"
     gene            complement(2100..3056)
                     /locus_tag="PKN31_05820"
     CDS             complement(2100..3056)
                     /locus_tag="PKN31_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOA99476.1"
                     /translation="MPANLTPQYREAEARYRSAKTPEEKLKALEEMLATIPKHKGTEK
                     LQAEIKQKISKLRKSKGERKSKGKLFDPYLIEKVGAAQIAIIGAPNSGKSTLLSLLTR
                     ARPEIADYPFTTHSPLPGMMEYEDIQIQLIDFPPWLEGRIEGNAIGALRRADGIIVVM
                     DGKSERLLDEMESLLQDLEENKFELEKEKTLILVNKIETPLEQENFSIFKELYEEEFA
                     IRGISFLTISAEEKESLKRDIFHLAGIIRIYSKPPGKPPDLSRPFVLKKGETVKDLAR
                     AIHKDLAYTLKGARVWGSAKFEGQLVTPDYVLQDKDIVEIKT"
     gene            complement(3063..3809)
                     /locus_tag="PKN31_05825"
     CDS             complement(3063..3809)
                     /locus_tag="PKN31_05825"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOA99477.1"
                     /translation="MKKYLWVVVILIVGISSIFYLRSRSLENKNNSVFREPWEEKCIG
                     MVEQQIEKRGVKDPLVLKAMREVPRHEFVPENLQNQAYDDTPLPIGYGQTISQPYIVA
                     LMTENLQIVPGCKVLEVGTGSGYQAAILARMGCQVYTIEIIKDLAEKAEEKLKELSYD
                     NVIVKQGDGYLGWEEEAPFDRIIVTCAVDHLPPPLLSQLKEGGKMVIPVGPPWSIQSL
                     WVVEKTRDGITTRDLGAVRFVPLTRESQGE"
     gene            3966..4454
                     /locus_tag="PKN31_05830"
     CDS             3966..4454
                     /locus_tag="PKN31_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HOA99478.1"
                     /translation="MYFHSPTQGRLSFKEAVISILNFIREEPQFHYRLIIGTDSRETD
                     DSPPAQLFVTAIVIHRVGWGARYFWRRTRTHSLYTIRDRIYQEALLSLQISQKLMDFL
                     DSFPDNHLSNYNVEIHVDVGNNGPTRSLIKEIVGMIEGMGFVAKVKPDSFGASRVAHR
                     HT"
     gene            complement(4533..5603)
                     /locus_tag="PKN31_05835"
     CDS             complement(4533..5603)
                     /locus_tag="PKN31_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOA99479.1"
                     /translation="MTVAVVMAGGRGERLWPLSRSYRPKQFVSWKGNSFFRATVDRIT
                     PVVGAENIYVVTQKKYKELALKQAPFISPSNVIIEPQGKNTAPCIGLAAVIISTLKSP
                     EEIMIVLPADHFIGDERTFQKLIRFACRLASDDFLVTLGITPLYPNTGYGYIHYGESH
                     QKEGEMEAFKVLNFTEKPDLETARKYLKEGTYLWNSGIFVWKAKVILEEIEKYMPDLY
                     RGLMKIKEKIGTPQEKEVASKVFKNLESISIDYGVMEKTDRALVIPADMKWSDVGSWV
                     ALEEILEKDEQGNVVLNKHLGIDTQNCIIWTRKPVVTLGVKNLVVVEADDVIFLMPKS
                     HNQRVRSVIEALKRQEEFKKLL"
     gene            6587..>7055
                     /locus_tag="PKN31_05840"
     CDS             6587..>7055
                     /locus_tag="PKN31_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOA99480.1"
                     /translation="MRHVLYYFLLALLVIVFVFPMYWTFTLSLKTHSDIVSPVPKFVF
                     KPTLMNYRGLFTGETGTTSLPASKPDFPRYFLNSFILSGMSTLLAIIIGVPAAYVLVR
                     GKFKEESTRESIAFTLLSFWFGPEIAIALPLYRIYQSIGLLDNYLGLILIYQLI"
BASE COUNT         1868 a         1404 c         1417 g         2366 t
ORIGIN      
        1 ttactcttcc tcttttaagc ggggttttaa ccatagctac tttgctttct attctctgga
       61 cttttaatga ttttaacgtt atttatattt taactaccgg tggtccaggc acttccaccg
      121 acattttaat aacttatatt tataagtatg cttttcaata tcttcggttt gggccggcag
      181 ctgctatggc ggtgataact tttgccatcc tgttaacagt tagcattatc tatgctcgaa
      241 attattttag aggaggggag gtattataag atgaaaaaga aaaactttag ttattattta
      301 gtttatccgg cgattatctt cattctttta gttcttttat ttcctttttt tgtgatgata
      361 tctacagctt tgaagcccct ggaagaagtt tataccactc cccctcactg gattcctaaa
      421 aaccccactt tagcaaattt ctctgatgtg tggttcaaat atcccctggg aagctatttt
      481 aagaacagtt taattgtggc agtaggtacc actataatca atactattct ctgtattccc
      541 gctgcctatg ctgtagctcg attacgattc cgggctaaaa gttttatcat gtatcttttt
      601 ttgatagtac agatgttttc tcccatcatt gtggttatat cgctatttaa aattattgct
      661 cgcctgggtt tgcttgataa tttattgtct ctcatcttca ccaacggggt tttcacttta
      721 gcttttgcta tctggctttt gagtggttac tttagtagtg ttcctaaaga aatagaggaa
      781 gctgctctta ttgatggttg ctctcgaatc caatcgataa caagagttct tttgcctgtt
      841 gctatgcctg gagtaattac tactgttatt tatactttta ttactgcctg gaatgaattt
      901 atgtttgccc ttacctttat tacctctatg gataaaagac ctctaacttt aggcctctat
      961 aattttgtgg gtcgctggac agtacagtgg caatatttga tggctgccgc tattctggct
     1021 ctcattccag tagtgattct ttttatgttt attgagaagg atttggttaa aggtctggca
     1081 ggaggggcgg taaaagggta gcttggaaac attcaatttt cagtggcaca ttaccaatcg
     1141 ttgtaacctt cgttgtcgcc attgctatca ggagaacttc caggattcag aaggaggaga
     1201 agggtatctt cttttggttg ctcaccgact gatggaagag ctggaaaaaa gagacctcta
     1261 taccactata aatataaccg ggggagaacc acttcttgtg ggggaagagc ttttctctct
     1321 ccttcactat cttaactctc atcctcgagt ggaagagctt atgattatta ccaatggcct
     1381 cctcttagaa aaaaacctgc tttttcgctt ggaagagtgc cagaagttaa ccactttgaa
     1441 gatatcttta gagggagcaa gttctttcac caatgacgct atcaggggac ggggcgtgtt
     1501 tgaaaaggta gtggacaaaa taaaactggc tcaagagatt tctttcctgg acctggtgct
     1561 catgtttact ttgagtaaaa gaaatgtaaa agagttatct tccatgttag cgctcgctca
     1621 aaatttagga gtggagggag tgatagtaga gcgcttcatc ccccagggaa gaggaaggga
     1681 aatgaaagaa gcagttcttt ctcgagaaga atggaggagt ttcttagagg agctcgtgaa
     1741 tgtctgtgag ctggaatgta ccccttatga gcttcttccc tacaaggctt tctggataaa
     1801 aggaggaaaa gaaggagaag tgctgggggc tttgtgcaat ttaagagagg cactttgtct
     1861 tatgcccgag gggactattt tcccctgtcg ccgttttcct tatcctctgg gtaatatttt
     1921 gaaagatggt ttagggaaag ctattgattc ccccttttta gaaaccttaa aagatagaaa
     1981 atatctaaaa ggccgatgta gctactgcca ggtagaaggt tgctatggtt gtcgggcact
     2041 cgcttatgct ctttttgatg acccctatta cgaagatgaa cagtgtttct ggaaagagac
     2101 tatgtcttaa tttctactat gtctttatcc tgtaaaacat aatcgggggt gaccagttgc
     2161 ccttcaaatt tagccgatcc ccacactcgc gctcctttta aggtgtaagc taaatcttta
     2221 tggatagcac gagctaagtc ttttaccgtt tccccttttt tcagtacaaa agggcgagaa
     2281 aggtcagggg gctttccggg gggtttacta tatatccgta taattcccgc taaatgaaaa
     2341 atatctctct ttaaactttc cttttcttct gccgagatag taagaaaaga aattccccga
     2401 atagcaaatt cctcctcata caattcttta aaaatggaga aattttcttg ttccagtggt
     2461 gtttctattt tatttactag aatcagtgtt ttttcttttt ccagctcaaa tttgttttct
     2521 tccaggtcct ggaggaggct ttccatttca tctaatagtc tttcactttt accatccatc
     2581 actactatta ttccatcagc tcttcggaga gcccctatgg cattaccttc tattcttcct
     2641 tctaaccagg gaggaaaatc gataagctga atctgaatat cttcatattc catcattccc
     2701 ggaagagggg aatgagtggt gaaaggatag tcggctattt ccggacgtgc acgagttaaa
     2761 agggagagga gggtagactt tccgctgttg ggagcaccga ttatggcaat ttgagcagcg
     2821 cctacttttt ctatgagata ggggtcaaat agttttcctt tgctttttct ttccccctta
     2881 gatttacgca gtttagatat tttttgcttg atttctgcct gtagcttttc tgttccttta
     2941 tgcttgggta ttgttgctaa catttcttcc agagctttta acttttcttc cggcgtttta
     3001 gcgcttcggt aacgggcttc tgcttcccgg tattgaggag ttaaattggc tggcaacttc
     3061 tctcactctc cctgagattc ccgggttaag gggacaaatc ttactgctcc taagtctcga
     3121 gtggtaatgc catctctggt tttttccact acccatagac tttgtatact ccagggagga
     3181 cctaccggga taaccatttt cccaccctct ttcagctggc tgaggagagg tgggggaaga
     3241 tggtctactg cacaggttac tattattcga tcaaaaggtg cttcttcttc ccagcctaaa
     3301 tatccatctc cctgtttcac tattacgtta tcatagctta actcttttaa tttttcctcc
     3361 gccttttcag ctaaatcttt aattatctct atagtataaa cctgacatcc catccgggct
     3421 aaaatagcag cttgataccc tgaacccgtt cccacttcca gcactttaca tccgggcaca
     3481 atttgtagat tttcggtcat caaagcgaca atataaggct gtgagatagt ctgtccgtat
     3541 ccaatgggaa gaggggtgtc atcgtaggct tgattttgca agttttcagg aacaaactcg
     3601 tgacggggca cctctcgcat ggctttgaga accagagggt ctttaacccc tcttttttca
     3661 atttgttgct ccaccattcc gatgcacttc tcttcccacg gttctctaaa aacagagtta
     3721 tttttgtttt ccaggcttct gcttcggagg tagaaaatag aagaaatacc cactattaaa
     3781 attaccacta cccagagata ctttttcata ttcgttattc ctgatattaa aaataaattt
     3841 ctgatttaat tttaacctta attaaggaaa atagtaagca aaaatatact tttagtgata
     3901 aaataagtga aataaaacta aagacgaggt gagttaaaga ccgcgattta gcaatttctc
     3961 aagatttgta ctttcatagt cccactcagg gccgcctgag tttcaaagaa gcagttattt
     4021 ccattctcaa ttttataagg gaagagcccc agtttcatta tcgattgatc attggaactg
     4081 attctcgaga gacagatgat tctccaccag cgcagctgtt tgttactgcc attgttatcc
     4141 acagggtagg ctggggagcg agatatttct ggcggagaac tcgtactcat agcttataca
     4201 ccatacgaga caggatttac caggaagcac tcctatcttt gcaaatttct cagaaattga
     4261 tggatttcct ggattctttt cctgataatc atctttccaa ctataatgtg gaaattcatg
     4321 tagatgtagg aaataacggt cccactcgta gtttgattaa agaaatagta ggaatgatag
     4381 aaggtatggg atttgtagct aaagttaagc cggattcttt tggggcttct cgggtggctc
     4441 atcgccatac ttaaagtaag tgattgactt tcatctgagt acggcatagc gcgaaaggta
     4501 aggcggaggg gaaatagagt ttattatcta aactaaagaa gctttttgaa ctcttcttgc
     4561 ctttttagag cctcaattac cgatcgcact ctctggttgt ggcttttggg cataagaaaa
     4621 attacatcat cagcttccac cactactaag ttttttacgc ccagagttac tactggcttt
     4681 ctggtccaga tgatgcaatt ttgggtgtca attcccagat gtttatttaa aacaacattt
     4741 ccctgttcgt ctttttccag aatctcttcc aaagccaccc aagaacctac atcactccat
     4801 ttcatatcgg cagggatgac cagagcccgg tcagtttttt ccattactcc ataatctatg
     4861 gagatgcttt ctaaattttt gaagactttg ctcgccactt ctttttcttg gggagtacct
     4921 attttttctt ttatcttcat aagcccccgg tataaatcag gcatatattt ttctatctct
     4981 tctaaaatga ctttggcttt ccacacaaat atcccgctat tccacaaata cgttccttct
     5041 ttcagatatt ttctggcagt ttcaaggtct ggtttttcgg taaaatttaa aactttaaac
     5101 gcttccattt ccccttcctt ttggtgggat tctccatagt gaatatatcc ataaccggtg
     5161 ttaggataaa ggggggtaat acctaaggtg accagaaagt catcacttgc cagacgacag
     5221 gcaaaacgga ttaacttctg aaaagttctt tcatctccta taaagtggtc ggcagggaga
     5281 actatcatta tctcttcggg actttttaaa gtgctgatta tgaccgccgc aagaccgata
     5341 cagggagcgg tatttttgcc ctgaggttct attataacgt tggagggaga gataaaagga
     5401 gcctgtttta gagccagttc cttatatttt ttttgagtta ctacgtaaat attttctgct
     5461 cctactactg gtgttattcg gtctactgtg gctcgaaaaa aactattccc tttccaggaa
     5521 acaaactgct tgggacgata gcttcggctc aagggccaca gcctctctcc tcgtcccccc
     5581 gccataacca ctgctactgt cattttatca ccttctttct tatattgtat caaaacaatc
     5641 caggttattc tcattttatt atatgttatt gcagatactg caaggctacg cgttatctct
     5701 tctgtttctt ttgagtgacg ttcgggagag atctttaaaa ataaatacat ctttacttta
     5761 tctgagtcgg gtgttttttt atagcaaggt atattaccct aagagatgtc taatacccag
     5821 gatgagagct tttgtctgga tagagtatta ctgatttctc atcttcagat gttcaatagc
     5881 catcagagct cccagcctat tatattataa actactggga aactggagaa agtagtatcc
     5941 aaaccggcag aaatggaaga acccgcgcat ttgacaatat ctttatagag atacttcacg
     6001 aatctccgag aagcttgctt aagagagacc agaaacgaac gggatgaaac ttctgtattg
     6061 atatcgggag tcggtcttat tatgttttct catactactg ctcccgattt aattatctcc
     6121 ataaaagctt ataagatttc tttaatagta gtataggaaa aatgatatta ctgtgcgact
     6181 gactatgtca cacatagaga agaatgtaac acaaagagga ggaaccaccg cggttttttg
     6241 cagttagcta taaaagcatg aggagaagaa ttttcaaacg gtcttcgcga cactgacaat
     6301 gtggttagtt gtattatact aaacaaggag gtggctggga gaaaaggaga aaaggcttcc
     6361 aaagtagttt attgctttgg aagccttttt gtttaagcaa acaaaaaagg aaggtggtat
     6421 cgagaaaatt attgaaaatt attattagtt aagggaaaag gtactactca gcaacaataa
     6481 actatgggaa ggactatcaa ttcctttgct ttttgacatt aatttttacc gttaaagatc
     6541 aaaggatttg aatataagaa ttgaatagga ggtgagaaaa ccttaaatgc ggcacgtcct
     6601 ctattatttt ttgctggctc tcttggtgat agtatttgtt tttcccatgt actggacgtt
     6661 cactctttcc ttaaaaactc acagtgatat tgtttcgccg gtgccaaagt tcgttttcaa
     6721 gcctacttta atgaattatc gcggtctttt tacaggcgag actggaacta cctctttgcc
     6781 tgcttcgaaa cctgattttc cccgttactt tttgaacagc ttcatcttaa gcggtatgag
     6841 cactctcctg gctattatta taggggttcc ggcagcttat gttttggttc gaggtaaatt
     6901 taaagaggaa tctactcggg aaagtatagc ttttaccttg ttgagttttt ggtttggtcc
     6961 cgaaattgct attgctttac ctctttatcg gatttaccag tctatagggc ttcttgataa
     7021 ttacttaggt cttattctca tttaccagct tattg
//
LOCUS       DAOPSS010000083         7013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8897_length_7013_cov_7.004168, whole genome shotgun sequence.
ACCESSION   DAOPSS010000083 DAOPSS010000000
VERSION     DAOPSS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8897_length_7013_cov_7.004168"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            681..1640
                     /gene="rbsK"
                     /locus_tag="PKN31_05845"
     CDS             681..1640
                     /gene="rbsK"
                     /locus_tag="PKN31_05845"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOA99481.1"
                     /translation="MSRPKILVVGSLNIDMILEIERMPEVGENLFVEKFSMLPGGKGN
                     NQAVACARLGALVSMIGKVGDDEFGERLFANLEQENVDFAFVTRQALTHTGLAFIFVD
                     EKGENRLLVAPGANMELSLTDLDNALELFKSNEFLVLQLEIPQEVVKKCIEYAHRSGM
                     KVVLNPAPAQPFPIEILTANDIITPNKYEAEVLSGVKIRSISDAIKAIQVLDERCPAR
                     KVITLGGEGALASYENNQFVYLPAREVKVEDTTAAGDAFNGGLVVGIGMGKDWIGALK
                     LANNAGAIACTKVGAQSALPHFRDVERLVERIGEGEVKIISGN"
     gene            1687..2964
                     /gene="hisS"
                     /locus_tag="PKN31_05850"
     CDS             1687..2964
                     /gene="hisS"
                     /locus_tag="PKN31_05850"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOA99482.1"
                     /translation="MSLIRVPKGTRDIWGEEIYLWHKVEEVVRSVAYSFQYEEIRTPI
                     FESTELFARGVGEVTDIVQKEMYTFPDKGGRSLTLRPEGTAPIMRAYLEHGLNISNPR
                     VKWFYMGPMFRYERPQAGRMRQFHQFGFEAIGFPQAACDAEIILIAWLVSKKLGLENL
                     SLEINNLGCNKCRDEYVKTLQDFLKGEGDNLCANCKIRAEKNPLRVLDCKEETCQKVI
                     HSPAFPLVQDFLCEECREHQDDLMEILRELDISFSINPYLVRGLDYYTRTAFEIKTGE
                     LGAQDAVAGGGRYDNLSSALGGPPVPGVGFAAGMERMILLLAKKYDPSSLKKQPLFYL
                     APLDVSGEKALLKLASFLGDRTIPFYLEFGDKRVKYHLKKAQKMGINTVIIAGKEEKE
                     RGELVWKDMVSGEQISMKEEELLAKLLEERKKC"
     gene            2958..4697
                     /gene="aspS"
                     /locus_tag="PKN31_05855"
     CDS             2958..4697
                     /gene="aspS"
                     /locus_tag="PKN31_05855"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOA99483.1"
                     /translation="MLRTHTCGELRKEDAGKEVQLTGWVSRLRDHGGILFIDLRDRWG
                     ITQLVVDNKEEIYTQASRVRPEYVIKVKGVVKERPEGLVNPRLSTGDVEIVVKDLEIL
                     SSSLLPPFEIEGNKETDESIRLRYRYLDLRRDKVQKNLILRHQVAQLIRRFLSEKEFL
                     EVETPFLTKSTPEGARDFLVPSRLSPGHFYALPQSPQLFKQILMISGMDRYFQIVKCF
                     RDEDLRADRQPEFTQVDIEMSFIEVEDIIQLIEAMMIYVFKESLGVAVDPPFPRLSYD
                     EAMEKYGTDKPDLRIPWEIDDLTELLGQEITSFSRGGNNFAVKALFLPQGEDFSRKKL
                     DVLSERAKENSLSLSWVKGGGSSPLKNKLSEETWQGMVNKYPFGENALLLLAWGERSN
                     LLNFMGSLRFELGEEWIKDKKPFQFCWITDFPLVDWNEEENRWDSVHHPFTSPREEDL
                     DFLDSHPEKVKSKTYDLVLNGYEIGSGSIRIHQMDLQRRIFNLLNLKEEEIEQKFGFF
                     IEALQYGCPPHGGIALGFDRLIMLMSGESSIREVIAFPKTQKGVCLLTGAPAPVEEKQ
                     LEILGISLQKQKK"
     gene            5686..6765
                     /locus_tag="PKN31_05860"
     CDS             5686..6765
                     /locus_tag="PKN31_05860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HOA99484.1"
                     /translation="MQVIYLMLPAAYFIAGILPILFWNRRTRVSSNIYLLGWLSWFCA
                     VSVKFFIATIQNLNWPIFYTNIPLMVINTTILEAIEVLSAFLFLKYHPALKEARDWKS
                     IVGFGLGFGCGEAITLGVIFLSSISIPFSLEFMVGGVLLSGFVERLSAIAIHLSSACF
                     LALFIVNSKKSYFIMGLLSKILSATMAAIFQVASLPWGLLPSIAFMEIAFAVYAIFWI
                     LILLYVRKKEEIAEELSPEQVVKIDRLNVLILSVIFFFFMYVWSIILKPLLTLPPFLH
                     ILSQVGLFFLVTIIIYPLLGKYRNASATEVAVGGAIALTLYTGAQNIIMSLQPNAGFL
                     TIAMFPFFGIICATGVYRLLIPIRD"
BASE COUNT         2070 a         1213 c         1666 g         2064 t
ORIGIN      
        1 gatagaacca ttcaggggca ggctaaaagc ggcttaaaga ttgccggggt gacgaagagg
       61 tgggaatcca aaggctttca atacttatgg acccctgata gaaccattca ggggtgacgg
      121 ataagggaca atagaggcac tcaggggtaa cgacaaaagg atgatagaac cattcagggg
      181 tgaccccccg ccgtcattcc cgaaatcttt tatcgggaat ccagaggcct ttgtctttaa
      241 ataatttaga tttctgatag agcattcagg ggtgacggca aaaaaggttc atgaggggaa
      301 agaatttccc cccttatacc gggagagaaa aagcgtctct cccggtagtc ttttgttttc
      361 ttaccttttt aatcttttcg ggggtcacag gggtactcct atagttcggt tatagttctg
      421 tgacaataaa agcaagaccg ccatgcgcaa gatgtggccg tgtccacgta atataaggat
      481 tctgtatttt ctcggtgtca ttgttttaat tcagtggaga tgagagctgc atccttactc
      541 ttccgttcca tatagtaaga tggtaaaatt ataaaggcta gcatgctaat aacttcttcc
      601 ttctttttct cctggaggcg gtaagatata gtagaatatt gcaaaacaac aggatttaca
      661 gataatacgg aggtgatacc ttgtctaggc cgaaaatttt agtagtggga agcttaaata
      721 tagatatgat acttgaaatt gaacggatgc cagaagtggg ggaaaatttg tttgtcgaaa
      781 aattttccat gcttcccggt ggtaaaggca acaatcaggc ggtggcttgt gctcgcctgg
      841 gagcactggt gagtatgatt ggtaaagtag gagacgatga gtttggagag cgcttgtttg
      901 ccaatttgga acaagaaaat gtagattttg cctttgtcac tcgacaggct ttgactcata
      961 ctggtttagc ttttatattt gtagacgaaa aaggggaaaa tcgacttctg gtggctcctg
     1021 gtgctaatat ggagctctcg cttaccgatc tggataacgc cttagagctt tttaaatcta
     1081 acgaattttt agttttacaa ctggaaatac ctcaggaagt agtgaaaaag tgtatcgagt
     1141 atgcccaccg ctctggtatg aaagtagtac tcaatcctgc tcctgcccag ccttttccta
     1201 tagaaatttt gactgccaat gatataatta ctccgaataa gtacgaagcg gaagttttaa
     1261 gtggagttaa aattagaagc attagcgatg ctataaaagc tattcaagtt ttggatgagc
     1321 gttgcccggc tcggaaagta attaccctgg gaggagaagg agcgctggca agctatgaga
     1381 ataatcagtt tgtctatctt cctgctcgag aggtaaaagt ggaggatact accgctgcag
     1441 gagatgcctt taatggtggg ctggttgtgg gaatcggtat gggtaaagat tggataggag
     1501 ctttgaagct cgctaataac gctggagcca ttgcctgcac taaagtagga gcgcagagcg
     1561 cccttcctca tttccgggat gtggaaaggt tagtagaaag gataggagaa ggcgaagtaa
     1621 aaataatttc gggaaactga gaagggaaaa ttttattttg ctcttgaaaa ttagaggagg
     1681 aaaataatga gcttaatcag agttcctaaa ggaactcggg acatatgggg agaggagatt
     1741 tatctctggc ataaagtgga agaagtagtc cgttctgtgg cttattcttt tcaatatgag
     1801 gaaataagaa cccccatttt tgaaagcaca gagctttttg cccgaggagt gggtgaagtt
     1861 accgatattg tacaaaaaga gatgtacacc tttccagata aaggagggcg tagcctgact
     1921 cttcggccgg agggaacggc tcccattatg agagcttact tagaacatgg tttaaatatt
     1981 tctaatcccc gagttaagtg gttttatatg ggtcctatgt ttcgctatga acgaccgcaa
     2041 gcagggagaa tgagacaatt tcatcaattt ggttttgaag ctattggctt tcctcaagct
     2101 gcctgtgatg cggaaattat tcttatagcc tggctcgtaa gcaaaaaatt ggggctggaa
     2161 aacctttctt tagaaattaa taacttaggt tgtaataaat gtagagacga gtatgtaaaa
     2221 actcttcaag atttcttaaa aggcgaggga gacaatctct gtgctaactg taaaattagg
     2281 gcagaaaaaa atcctctccg ggttttagat tgtaaagaag aaacttgcca gaaagttatt
     2341 cattctcccg cttttccttt agtgcaagat tttctatgtg aggaatgtcg cgagcatcag
     2401 gatgatttga tggagatttt aagagaatta gatatttctt tctccatcaa tccttattta
     2461 gtaagaggtt tagattatta tacgagaact gcttttgaaa ttaaaactgg ggagctggga
     2521 gcgcaagatg ctgtggcagg aggaggaaga tatgacaatt tatcttctgc tttaggaggc
     2581 cctcctgtac cgggggtagg ttttgctgct ggaatggaga gaatgattct tctcttagct
     2641 aaaaagtatg acccctcttc tcttaaaaag cagcccttgt tttatctcgc tcctttagat
     2701 gtttcgggag aaaaagccct gttgaagctg gcttcatttt taggggatag aactattcct
     2761 ttctatttag agtttggtga taaaagagta aaatatcatc tcaaaaaagc tcaaaagatg
     2821 gggattaata ctgtgattat agcggggaaa gaagagaaag agagaggaga gctggtgtgg
     2881 aaggatatgg tttcgggaga acaaatatca atgaaagaag aagagttatt agctaaatta
     2941 ttggaggaaa gaaaaaaatg ctgaggactc atacctgtgg tgaattgaga aaagaagatg
     3001 caggaaagga agtgcaatta actggttggg tgagccgttt gcgcgaccat ggagggattt
     3061 tgtttatcga tttgcgagat agatggggta ttacccagct cgtggtagat aataaagaag
     3121 aaatttatac tcaagcttcc cgggttaggc cagagtatgt tataaaggta aagggagtgg
     3181 taaaagagcg tcccgaaggg ttagtcaatc ctcgactctc tactggagat gtggagattg
     3241 tagtgaaaga cttagaaatt ttatcttcct cgctcctccc tccttttgaa atagagggaa
     3301 ataaggaaac agatgaatct atccgccttc gctatcgcta tctggacctt cgccgggata
     3361 aggtgcagaa aaatttaatt cttcgccacc aggtagctca gctaatacgt cgattccttt
     3421 ctgaaaaaga attcctcgaa gtggagaccc cttttcttac taaaagcact cctgaaggag
     3481 cccgggactt cctggttccc agcaggctca gtccaggtca tttttatgcc cttccccaat
     3541 ctcctcagct tttcaaacag attttgatga tttctggtat ggatcgttat tttcagatag
     3601 ttaagtgctt tcgagatgaa gacttgcgag ctgaccggca accagagttc actcaggtgg
     3661 acatagagat gtcttttata gaggtggaag atatcatcca gttaatagaa gcaatgatga
     3721 tttatgtttt caaagagtct ttaggggtgg cggtagatcc tccttttcct cgactttcct
     3781 atgatgaggc gatggaaaaa tatggtacag ataaacccga tttgcgcatt ccctgggaga
     3841 ttgatgacct tactgaactt ttagggcagg agataacttc tttttctcgg ggaggaaata
     3901 actttgctgt taaagctctt ttcttacctc aaggggaaga tttctctcga aaaaaattag
     3961 atgttctttc tgagagagca aaagaaaact ctctttctct atcttgggtt aaaggagggg
     4021 gttcctctcc cttgaagaac aaactttctg aagagacctg gcaaggaatg gtaaataaat
     4081 atccctttgg agaaaatgcc ctgcttcttt tagcctgggg agagaggtct aatttactga
     4141 attttatggg tagtttgcga tttgaattgg gtgaggagtg gataaaagat aagaaacctt
     4201 ttcaattctg ctggataact gattttcctc tggtagattg gaatgaagaa gaaaatcgct
     4261 gggactcggt tcatcatccc tttacttctc cccgggaaga agaccttgat tttctggata
     4321 gtcacccgga aaaagtgaag tccaagactt atgatttagt cttgaatggt tatgaaatag
     4381 gctcggggag cattcgtatt caccagatgg atttgcaaag aagaattttt aatcttttga
     4441 atttaaaaga agaggaaatt gagcaaaaat ttggtttctt cattgaagct ctgcagtatg
     4501 gttgccctcc tcatggggga attgctctgg gttttgaccg attaattatg ttgatgagcg
     4561 gggagagttc tattcgagaa gttattgctt ttcctaaaac ccagaaaggg gtatgccttc
     4621 tcactggagc tcccgccccg gtagaagaaa aacaactgga gattttaggt atatctctcc
     4681 aaaaacaaaa gaaatgataa ccatcgtcgt atctttttat catgaagttt ctgggttaat
     4741 ggacaaattt acctatgtag tctataaata aatttatata gtattctaaa cctgttaaat
     4801 tgcacaaatt ttaaaagcaa gttatattag aagttaaaag ctcgggatca cgacagaaaa
     4861 gggaaaggag gaccccttat aatcccccga aagacaaaaa tagagcaacc tgcggagtca
     4921 ataaagaaat aataaaaaga caagagctac tgagaaagga aatctccctc agcgaggtga
     4981 actctctata accccgaaag agaaaaaaca gacggcctac agcgtaaggt taaaacatga
     5041 ataaaaatac aaaatactac tggtagaaaa atcatccacc agtataaagg ggaaagaatt
     5101 cctcccttat gaccccccct tcttttgccg tcactcctga atggttctat cgcttttttc
     5161 gattatgctc tatatcctcg taaaatagaa aagtggatgg cctttgtggt aaagcaagtc
     5221 ttatagaaaa ttttagagaa aggaggccat ccaccgaaag aagaggagct atgaagactg
     5281 gctatatttt ctgcaagatt agtaaaaaga ggattgagag tgcctacctt cataacgagt
     5341 tgcggtgctc ccggactaat caaggctatt gaagtagtat tctaaaagta tcaaaggaag
     5401 atgctggttt catagaatgg agaatttctc tcataattaa ctccttagac aacttaagaa
     5461 agaatttggg tattactgac ggattatcag aaaaagaact actgaaggag aggagatgag
     5521 atgaggtgag gtgaggtgat gagaaggcta tccattttta cggcaaaaaa ggacttgggc
     5581 ataggcggcg gcaatatcgt tggagaagta gtacaataag acgaggcatc gttttagctg
     5641 tcgatttcag aggaaaaaat aaggataaaa gggaggtcgc tacctgtgca agtcatatac
     5701 cttatgcttc ctgcagctta ttttatagct ggaattctgc ctattctgtt ttggaataga
     5761 cgtaccagag ttagttccaa tatatatttg ttgggctggt tgtcctggtt ttgcgcagtt
     5821 tctgtaaagt tttttattgc taccattcag aacttgaact ggcctatttt ttataccaat
     5881 atccctctga tggttataaa tacgaccatt cttgaagcca tagaggttct ctccgctttt
     5941 ttattcttaa agtatcaccc agcactgaag gaggcgcgag actggaagag catagtgggg
     6001 ttcggtcttg gctttggttg tggcgaggct ataacgctgg gagtgatatt tctttcttcg
     6061 atatctatac cattttctct ggaatttatg gtgggaggtg tccttttaag tggttttgta
     6121 gagcggctca gtgccatagc gatacatcta tcgtctgcat gtttcttagc attattcatt
     6181 gtaaacagta aaaagagcta ctttataatg gggttgctat caaaaatttt atctgcaacc
     6241 atggcagcaa tatttcaagt cgcatctctg ccctggggtt tattaccctc tatagcgttt
     6301 atggagattg cctttgctgt ctacgctatt ttttggattc taatattatt atacgttaga
     6361 aaaaaagagg agatagctga ggaattgtca ccagagcaag tagttaaaat agacagatta
     6421 aacgttctca ttctttcagt tattttcttc ttttttatgt atgtctggtc gataatatta
     6481 aagcctcttt tgaccttgcc tccttttttg cacattctct cccaggtggg gttatttttc
     6541 ctcgttacca tcattatata tcctcttttg ggaaaataca ggaatgcatc tgctactgag
     6601 gttgcagtcg gtggtgctat agcgctgact ctttatactg gagcacagaa cataataatg
     6661 agcctgcagc ccaatgctgg ttttctcaca atagcaatgt tcccattttt tgggattatc
     6721 tgcgccaccg gagtttatag gcttttaatc ccgatcaggg attgaaaatg gggtttgctt
     6781 gctggcaaca tgtagcaagc aataagagct tcgggctttt tcctaccctt acctggattt
     6841 actacaagaa cctcttttgc ctgtgaatat tgcgtgtccc ttgtgcgtgg cgatctcgct
     6901 tttcaagaat ggagaattgc catacaataa taccggagta cagaaaagag taaaggacgg
     6961 ggaagaaaga ttaccgtgga ttttgcggtt gagtataata gataaaaaag gag
//
LOCUS       DAOPSS010000084         6984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_8944_length_6984_cov_7.184009, whole genome shotgun sequence.
ACCESSION   DAOPSS010000084 DAOPSS010000000
VERSION     DAOPSS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8944_length_6984_cov_7.184009"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKN31_05865"
     CDS             <1..516
                     /locus_tag="PKN31_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99485.1"
                     /translation="AYRRTAADFDETEHLKEHLHLLKQVRSPFYLDLDDFDRILRWKL
                     RQQYARQKSLRSKNTEDIIRDITCLAFNISHEDEDYELELRLRLLTALRGVGIPVASA
                     VLALCYPEQYAVIDFRGWRQMFGVEKRNFSINDYKRYLKEIKRLAAELGWLPQEVDLA
                     IWEYDRQSGRR"
     gene            532..729
                     /locus_tag="PKN31_05870"
     CDS             532..729
                     /locus_tag="PKN31_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99486.1"
                     /translation="MGKYRNTSATEVAVGGAIALTLYTGAQNIIMNLQPNAGFLTIAM
                     FPFFGIICATGLYRLLILTRD"
     gene            complement(812..974)
                     /locus_tag="PKN31_05875"
                     /pseudo
     CDS             complement(812..974)
                     /locus_tag="PKN31_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
     gene            1121..1354
                     /locus_tag="PKN31_05880"
     CDS             1121..1354
                     /locus_tag="PKN31_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99487.1"
                     /translation="MIVKIEARSHQAPKVQECLTRYGCNIKTRLGLHETSPEYCAEDG
                     LVILELTGKKEENEQLKKELESLEGVTVQYLEI"
     gene            complement(1329..2315)
                     /locus_tag="PKN31_05885"
     CDS             complement(1329..2315)
                     /locus_tag="PKN31_05885"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HOA99488.1"
                     /translation="MLWPPEWSFLPHALNNFRGTLMVVAPPDRGKTTLIRLLTSFFVS
                     QGKKVGWVDSDLGQSNVGPPTTVGMIMVSKKFPLSLRERLFTPYFRFVGDVTPEKRIE
                     ATALYAAELAKMASSKSQVTLIDTCGLIFSLSGLRLKMLKMKSIKPELVLGIGYDKEF
                     EVLKKFYGDQFWTLPPSSQTSRKDYEKRRSYRERLFSLYFSSGKTWEMGWENFRFFSP
                     YHPYFFLQEYQLSDGTPTFKFSSTFSLILSAFPEEKVNNKCRVINPSSLLFSICGLYT
                     ERGEEVGLGIIQNINEENKTVAVKGIRINPGKPEALVPGVTKINAKSPDIEQ"
     gene            complement(2327..3025)
                     /locus_tag="PKN31_05890"
     CDS             complement(2327..3025)
                     /locus_tag="PKN31_05890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOA99489.1"
                     /translation="MRKLKILVWISCLILLILFLQEEAISIETEEDIFGLNLVRSLGG
                     NSQVRKEVALTFDDAPSPFTKEVLKILDNYGVSATFFLVGSQVEKSPEVARQIVAAGH
                     EIGNHSFSHCWSSDDSVETLVEDIKRAEEAIYRATGQVPLYFRPPGGMVNDKVKEACG
                     QTGYGILLWWVDSRDWLLTEEEILEKVKKEVKDGAIILFHNLATTVEVLPQVIEDLRR
                     EGYKFVTISQLLRY"
     gene            complement(3025..3345)
                     /gene="trxA"
                     /locus_tag="PKN31_05895"
     CDS             complement(3025..3345)
                     /gene="trxA"
                     /locus_tag="PKN31_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOA99490.1"
                     /translation="MIELNKDNFKEEVLDSEIPVLVDFWATWCTPCRMMAPIIEDIAN
                     SWQGKVKVCKLNTDDAPEIAIKYGIQAIPTLLIFNGGKEVGRIVGYVPKKTVEEKLQD
                     IIKP"
     gene            complement(3351..3944)
                     /gene="plsY"
                     /locus_tag="PKN31_05900"
     CDS             complement(3351..3944)
                     /gene="plsY"
                     /locus_tag="PKN31_05900"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOA99491.1"
                     /translation="MVNWWVLPLNYLLGSFPCGLIVGWITKGVDIREYGSGSIGATNV
                     SRVAGFLPALIAGVGDASKGFLGAYLATRFLPDPYLWFLALFLLIVGHDWSIFLRFRG
                     GKGVATTLGILFYLSWESALLCFLVWVVMASITRYSSVGSLSGALLMPVFLYLWGKPP
                     EFIVWGIFAAALVFWRHKGNIQRLIQGKELKMGQKGA"
     gene            complement(3950..5191)
                     /gene="fabF"
                     /locus_tag="PKN31_05905"
     CDS             complement(3950..5191)
                     /gene="fabF"
                     /locus_tag="PKN31_05905"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOA99492.1"
                     /translation="MERNVVVTGIGVISPVGSGKDKFWQSLIGGKSGIDRIKSFDPGD
                     FDSQMAGEVKDFDPEDFLPRKEYRRMDRFSQFAVCASLMAMQDASISLENLDRERAGV
                     ILGSGIGGIQTFEEQHQILLTKGPGKVSAFFIPMMIVNMGAANVAIQLKLKGPNSCVS
                     TACEASTHALGEALRIIQRGEADLMVVVGAEASITPLAFAGFCAMKALSTHNDNPAKA
                     SRPFDRTRDGFVMAEGAAALILEEEDKARERGARIYAELKGYGSTCDAYHITAPDPEG
                     EGAARSMKLAIEDARLTTEDIDYINAHGTSTPLNDKVETLAIKKVFGERAYQIKISST
                     KSMTGHLLGGAGALEGAATALSIYHGIIPPTINLEEKDPECDLDYTPGQAFPLKIRSA
                     LSNSFGFGGHNGTLVFCRYGE"
     gene            complement(5205..6188)
                     /locus_tag="PKN31_05910"
     CDS             complement(5205..6188)
                     /locus_tag="PKN31_05910"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOA99493.1"
                     /translation="MSNGEVKILGTGSSVPERIITNFDLEKMVDTSDEWITTRTGIKE
                     RRIAQEEESTSTFALLAAQRALQAAQVSPEELDLIIVATVTPDMLFPATACLLQRELK
                     AHKAASFDLEAGCTGFVYALSLAEKYLRGGGGEKALVVGAETLSKILDWEDRSTCVLF
                     GDGAGAAVLGLSEEEGIIATYLGSDGGGAHLLELPAGCSRMPASLDTVNSRLHYIKMN
                     GNEVFKFAVKIMEEASLEVIKRGQVEIDEVSLFIPHQANIRIINSAAKRLGIPEEKVF
                     VNVHKYGNTSSASVPLALDEAYREGKIKRSDLVLLVGFGAGLTWGSALIRW"
     gene            complement(6185..6625)
                     /gene="fabZ"
                     /locus_tag="PKN31_05915"
     CDS             complement(6185..6625)
                     /gene="fabZ"
                     /locus_tag="PKN31_05915"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOA99494.1"
                     /translation="MELDVNKIQEILPHRFPFLLVDKIISLENDKVVGVKNVTMNEWF
                     FQGHFPGKPVMPGVLILESMAQVGATMMMNMEEFRGCIPYFTSLNGVKFRRPVIPGDQ
                     LVMEVTLLKVRRGIGKLKGVARVNETIVAEGEIGFSLIKEEAED"
BASE COUNT         1963 a         1635 c         1421 g         1965 t
ORIGIN      
        1 gcataccgga gaactgcagc tgactttgac gaaactgagc atttaaaaga acacctgcat
       61 ttgttaaagc aagttcgtag tcctttttat ttagacctcg atgattttga tagaatttta
      121 agatggaaat tgagacaaca atacgcaaga caaaaaagtt tgagaagcaa gaataccgaa
      181 gatattataa gggatattac ctgtttagct tttaatatct cgcatgagga tgaggattat
      241 gagctcgagc ttcggctccg attattgact gcccttagag gagttggaat cccggtagct
      301 tctgctgtgc tcgctttgtg ttatcccgag caatatgcag ttattgattt tagagggtgg
      361 cgccagatgt ttggggtgga aaaacggaac ttttccatca atgattacaa acgatacttg
      421 aaagaaatca agagacttgc tgctgagctg ggatggttac ctcaagaagt ggacttagca
      481 atctgggagt atgaccgcca gagtggtaga agataaatta tatatcctct tttgggaaaa
      541 tacaggaata catctgctac tgaggttgca gtcggtggtg ctatagcgct gactctttat
      601 actggagcac agaacataat aatgaacctg cagcccaatg ctggttttct cacaatagca
      661 atgttcccat tttttggaat tatctgcgcc actggacttt atagactttt aatcctgacc
      721 agggattgaa aacggggttt gtttcaaact ctgtatcctt aagtggttga gcaacttcta
      781 cgggaaggtt ctcgtcaact ttgaatttca tttagctacc cgattcaata acaatatggc
      841 actctttggc taaaagtgca gcataagcca atgcggcact aatatcttcg ggatgaaaga
      901 gaaggataac ctttcaaaat ctcttcccgg ctaactcctt cagcgaggtt atccagtatt
      961 actgagatag gaatcccaac ccgtaaagag gccgaagatt tccttaatac gagctcaact
     1021 gattagaatg gttttctgaa gagagaggtg cttcccctgc accgggcaga tattttctat
     1081 gatataattg aacagggtgg taaaattggc ttatcaaatc atgatagtga aaatagaggc
     1141 tcgctctcac caggcgccta aggtgcagga gtgcttaacc agatacggct gcaacattaa
     1201 aacccgtttg gggcttcacg aaacctctcc ggaatactgt gccgaagacg gactggtaat
     1261 cttagaatta accggtaaaa aggaggaaaa cgaacagctg aaaaaggagc tggaaagttt
     1321 agagggagtt actgttcaat atctggagat ttagcattga tttttgttac tccaggaact
     1381 agcgcttccg gtttaccggg gtttattcta atccccttca cagctactgt tttattttct
     1441 tcattaatgt tctgaatgat gcccagaccc acttcttctc ctctttcggt gtaaagcccg
     1501 cagatggaaa agagcaagga agaagggtta ataacccggc atttattatt tactttttct
     1561 tctggaaaag cactgaggat gagggaaaag gtggaggaga atttgaaagt aggtgtgcca
     1621 tcgcttagct gatattcttg caggaagaaa taaggatgat aaggtgagaa gaaacggaaa
     1681 ttttcccacc ccatttccca agtttttccg gacgagaaat ataaagagaa aagccgttcc
     1741 ctgtagctgc gcctcttctc ataatctttt ctagaagtct gggaagaggg gggtaaagtc
     1801 cagaactggt ctccataaaa ctttttcagt acttcaaatt ctttatcata tccgatacct
     1861 aagactaatt ctggcttgat cgatttcatc tttaacattt tgagacgaag accagataaa
     1921 gaaaaaatca aaccacaggt atcaatcaga gtaacctgcg atttagaaga agccattttg
     1981 gctaactcag cagcatataa agcagtagct tctatccttt tttctggagt tacatctccc
     2041 acaaagcgaa agtagggagt gaataacctt tctcgaagag aaagggggaa ttttttagac
     2101 accattatca tccctacagt ggtaggagga ccgacattag attgccccag atctgaatct
     2161 acccatccca cttttttacc ttgagagaca aaaaaagaag tgagcaagcg gattaaagtg
     2221 gttttccccc ggtctggtgg agccactacc ataagtgttc ctcgaaaatt attcagagca
     2281 tggggcaaaa aactccattc cgggggccag agcatctttt ttacctctaa tatctgagga
     2341 gttgagagat ggtaacaaac ttataacctt ctcttcttaa gtcctcaata acctggggca
     2401 gaacctcaac agttgttgct aagttgtgaa aaagaatgat agctccgtct tttacttctt
     2461 ttttgacctt ttccaggatt tcttcttcag tgagcaacca atctcgagag tctacccacc
     2521 acaataagat gccgtagccg gtttgcccac aggcttcttt tactttgtca tttaccattc
     2581 cccccggagg acggaagtat agagggacct ggccggtagc tcggtaaata gcttcttctg
     2641 cccgcttaat atcctctacc agggtttcca ctgaatcatc actcgaccag caatgagaga
     2701 atgagtgatt acctatttca tgtcctgcag ctacaatctg gcgagcaact tcaggagact
     2761 tttctacttg agaaccaacc agaaaaaaag tagctgatac tccataatta tctaaaattt
     2821 tcaacacttc tttagtaaaa ggactgggag catcatcaaa agtcagtgct acttcctttc
     2881 tgacttggga gtttcccccc agagagcgaa ccagattgag accaaagata tcctcttctg
     2941 tttctatgct aattgcttct tcctggagaa aaagaattaa aaggataaga catgaaatcc
     3001 aaactaatat ttttaatttg cgcatcaggg ttttatgata tcttgcagtt tttcctccac
     3061 agttttttta ggtacatatc ctactattct tcccacctct ttacctccgt tgaaaattaa
     3121 aagggtaggg atagcttgaa tgccatattt aatagcaatt tccggagcat catcggtatt
     3181 taatttacaa actttgacct ttccctgcca ggaattagct atgtcttcta tgataggagc
     3241 catcattcgg cagggcgtgc accaggtggc ccagaaatca accaatactg gaatctctga
     3301 atccagcact tcttctttga aattatcttt gtttaattct atcatgttct ttacgctcct
     3361 ttctgaccca tctttaactc tttaccctgg attaacctct ggatatttcc tttgtgcctc
     3421 cagaacacga gcgctgcggc aaatattccc cagactataa actctggagg ctttccccac
     3481 aggtaaagga aaacgggcat caaaagagca ccgctcaggg aacctacgga ggagtagcgg
     3541 gtaatggatg ccatgaccac ccagacgaga aaacaaagaa gcgccgattc ccaggagagg
     3601 taaaaaagta tccctaaagt agtggctact cctttccctc ctcggaagcg gagaaatata
     3661 gaccagtcat gaccgacaat caagagaaaa agagctaaaa accagagata gggatcgggt
     3721 agaaaacgag tggctaaata agcgcctaaa aatccttttg aggcatctcc cacgccggcg
     3781 ataagagcgg gcaggaaacc tgctacccgg gaaacattgg tggcaccaat actgccactc
     3841 ccatattctc ttatatccac acctttggtt atccagccca ctatcaaccc acaagggaaa
     3901 gaacctaaga ggtagttgag aggcaagacc caccagttga ccattgatat cactctccgt
     3961 agcgacaaaa gactaaagtg ccgttatggc ctccgaatcc gaaagaattg gaaagagcgc
     4021 tcctgatttt aagaggaaaa gcttgaccgg gggtgtaatc cagatcgcac tccgggtctt
     4081 tttcttccag attaatagtg gggggaatga tgccgtgata aatggaaaga gcagtagctg
     4141 ctccctccag tgctcctgct ccccccagaa gatgtccggt catagattta gtggaactaa
     4201 ttttaatctg gtaagctctt tctccaaaaa cttttttaat ggccagggtt tccactttat
     4261 catttaaggg agtagaggta ccgtgagcat tgatataatc gatatcttct gtggttaagc
     4321 gggcatcttc tatcgccagc ttcatagacc gggctgctcc ttctccttct ggatcagggg
     4381 cagtaatgtg gtaagcatcg caggtggagc catatccttt taactcagcg taaattcgtg
     4441 ctcctcgctc tctcgccttg tcttcctctt ctaaaattag tgctgctgct ccctcggcca
     4501 ttacaaatcc gtctcttgtg cggtcaaaag ggcgggaagc tttagccgga ttatcattat
     4561 gggtggaaag ggctttcata gcgcaaaatc cagcaaaagc taaaggagta atagaagctt
     4621 ctgctcccac tactaccatc aagtctgctt ctcctctctg aatgatgcgc agagcttctc
     4681 ctaaggcatg ggtagaagct tcgcaagcgg tagaaacaca actattgggt cctttcagct
     4741 tcaactgtat cgccacattg gctgccccca tgttaactat catcatggga atgaagaaag
     4801 cactgacttt tcccggacct ttagttaaaa gaatctggtg ctgttcttca aaagtctgga
     4861 ttcccccgat gcctgagcct aaaataaccc ctgctctctc tctatccaga ttctcaagag
     4921 atatggaagc atcttgcatg gccataaggg aagcacaaac tgcaaactgg gaaaaacggt
     4981 ccatcctccg gtattctttg cgggggagga agtcttcggg gtcgaaatcc tttacttctc
     5041 cggccatctg actatcaaaa tctccagggt caaagctttt aattctatca attcctgatt
     5101 tacccccgat gagcgattgc cagaatttat ctttgccact acctaccgga cttattactc
     5161 ctatgcccgt gactactaca tttctttcca ccttaaaacc tcctttacca tcggataagg
     5221 gctgagcccc aggtcaaacc ggcgccaaaa ccaaccagga gcactaaatc actccttttg
     5281 attttcccct cccggtatgc ttcgtccaaa gccagaggaa cggaggctga ggaggtgtta
     5341 ccatatttat gaacattcac aaaaactttc tcctcgggaa tgcccagcct tttagctgcc
     5401 gaattaatga ttctaatgtt ggcctgatga ggaataaaaa ggctcacctc atctatttct
     5461 acctggcctc tttttattac ttctaaggaa gcctcttcca taattttcac cgcaaattta
     5521 aatacttcat ttccattcat ttttatatag tgcaatcggc tattaacggt atccagagaa
     5581 gctggcattc gagaacaacc ggcggggagt tccagaagat gagctcctcc tccatcagag
     5641 cccaggtaag tagctataat tccctcttct tcgctcaacc ccaggactgc tgctcctgcc
     5701 ccatcgccga ataacacaca ggtagagcga tcctcccagt ctaatatttt agaaagggtc
     5761 tctgctccca ccactaaagc tttttctccc cctccccccc ggagatattt ttcagccagg
     5821 gataaagcgt aaacaaaccc tgtgcaacct gcttctaagt caaaactggc ggctttatga
     5881 gcttttaact ctctctgcaa taagcaggct gttgcaggaa aaagcatatc aggagtaact
     5941 gtagccacta ttatgaggtc taactcttca ggagaaactt gagcagcctg aagcgccctt
     6001 tgagcggcaa gcaaggcaaa ggtagaagta ctttcttcct cctgtgctat tcttctttct
     6061 ttgataccgg tgcgagtggt aatccattca tcgctcgtat ctaccatttt ttccaggtca
     6121 aaattagtaa taattcgttc tggcacactg gagcctgttc ctaatatttt tacctcacca
     6181 ttgctcaatc ttctgcctcc tcttttatca gagaaaaacc tatttctcct tctgctacta
     6241 ttgtttcatt cactcgtgct actcctttta acttaccaat tcctctccgc accttgagga
     6301 gagttacctc catcactaac tggtctcctg gtataaccgg acggcggaat ttaacaccat
     6361 ttaaagaggt aaaataagga atacatcccc gaaactcttc catgttcatc atcatagtgg
     6421 cacctacttg agccatgctc tctaaaatca aaactcctgg cataacaggt tttcccggaa
     6481 aatgcccctg gaaaaaccac tcattcatag tcacattttt tacccccaca actttatcgt
     6541 tctccagaga tattatttta tctactaaga ggaaaggaaa gcggtgaggc agtatttcct
     6601 ggattttatt tacatctaat tccatttcca catcctcccg attagcatat tttaccccta
     6661 ataatgtata tgaactgagt caaattagca aggagcaata tcgattatta taattaactg
     6721 caaaatccat ggcaacatcc ttatattcgg tactccattt atctgtattg tactccgggg
     6781 cttcccctcg cacggcggtg cagaacggag ctatgaggag gctatctccc ttcatagtca
     6841 atttcaccct aaccctcttc ccggaaggat ttccctcctc ccgtcgtttc cgagatcttc
     6901 agtaggtatc caccccttct gtcgtcgttc ccgaaatttt tagcggggaa tccatgtatt
     6961 taaaaaccat ggacccctga taga
//
LOCUS       DAOPSS010000085         6900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_9092_length_6900_cov_8.577064, whole genome shotgun sequence.
ACCESSION   DAOPSS010000085 DAOPSS010000000
VERSION     DAOPSS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9092_length_6900_cov_8.577064"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1214
                     /locus_tag="PKN31_05920"
     CDS             9..1214
                     /locus_tag="PKN31_05920"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOA99495.1"
                     /translation="MGRQKVVLAYSGGLDTSVAIRWLEEQFNAEVYALTIDVGQGKDV
                     EEVRKKALSIGAKEALVFDAREEFLNEYVLPALWAQAIYEDRYPLATALSRPLIAKYL
                     VKVAEEKGATMIAHGCTGKGNDQVRIEVSVMALNPDLQVVAPARQWGWSREEEIEYAH
                     ANGIPVPTTLEQPYSIDQNIWGRSIECGVLEDPLVEPPAEVFEWTRDPKDAPSEPAYL
                     EIGFEEGVPRLLGGEYYSLLSLVEELNKIGGENGFGRVDHVENRLVGIKSREIYECPA
                     ALILLEAYRDLQNLVLPREVIHFKPHLEEKYAQLVYDGLWYSPLREALDAFMKSFLPR
                     MTGIVRVKLYKGSMQVVGRKSEYSLYDWGLATYDRGDLFDHQASEGFIKIWGLPARVQ
                     ATLERRKKS"
     gene            1211..2665
                     /gene="argH"
                     /locus_tag="PKN31_05925"
     CDS             1211..2665
                     /gene="argH"
                     /locus_tag="PKN31_05925"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOA99496.1"
                     /translation="MTNLWGSRMEKGLDQEVKEFSTSIPEDFFLYKYDLWGSAAHCKM
                     LEKVGIISEKESKEILRGLRVIFQEIEEGKVDPSPFEDIHSLVEIRLREIAGEKIGGK
                     LHTARSRNDQVVLDERLFLRERIGEVIEKISFLQEALLRKAQENKDVVMPGYTHLQPA
                     QPVLFAHHLLAYFFMFQRDIERLEDTRKRVNVLPLGAGALSGTSLPIDRQYVAELLAF
                     PRVQENSMDAVSDRDFILEFLGGLSILFLHLSRWGEEIVLWSSPAFHFLEIDDAFTTG
                     SSIMPQKKNPDVAELLRGKTGEVLSSWVSLAITLKGLPLTYNRDLQQDKPPLFRATKE
                     TLSSLRIAARLTQNIFPDRESMKKALSVGFLTATDLCEYLVGQGVPFRRAHAWVGEMV
                     KKLSASGKHLRDLKKEDWGEYQHLFPENWEEIIDEEKSVRRKISEGSTSPREVEREIK
                     IGEELLAQSRERVSSWRKIEQESWENLMGGSCVL"
     gene            complement(2881..4737)
                     /gene="mnmG"
                     /locus_tag="PKN31_05930"
     CDS             complement(2881..4737)
                     /gene="mnmG"
                     /locus_tag="PKN31_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOA99497.1"
                     /translation="MEEYYDVIVIGGGHAGCEAAHASARMGAKTLLLTTSIHHLALMP
                     CNPAIGGPGKGHVVREIDALGGLMAKATDLSTIHIRRVNTGKGPAVQTLRAQTQRDLY
                     SLIMRKFLENTPHLYLFQGEATEILQDEKGEIKGVKVKNQTTFFAPAVVLCTGTFLNG
                     LIKIGELSYPAGRQGEFPSIDLACNLRNMGLQSGRLSTCSPPRLDRRTIDFSSLKEQK
                     SDDKPLCFSFTGVPQVYEGASVFITRTSKKTCDIIKANLHRSPLLYSISDTSSVRECP
                     SLEDKVYRFPERENHLLFLEPEGKKSNEIYVQGLFTSLPEEVQREIVHSITGLERAHI
                     IRPGYGIEYDYILPTQLKSSLECREIPGLFLAGQINGTSGYEEAAGQGIIAGINAASY
                     VKGQPPLILRRDESYIGVMIDDLVTKGVDEPYRLRTGKVEFRLVVRHSNADLRLSFYA
                     YERGIISEKEYHRVEEKRDKIQKEIERLSRLTIYPTTQLQSLLTQKGTNPVKEAIKAS
                     ALLSRPEISYRDLAPFDPERPFLEPQVIEEVEIEIKYRGYIERQKREIEEFKKMENKV
                     IPPDFDFSSIQVISQEAREKLQKVRPATLGQAARVAGVTPSDIAALRILLEK"
     gene            complement(4771..6321)
                     /gene="purH"
                     /locus_tag="PKN31_05935"
     CDS             complement(4771..6321)
                     /gene="purH"
                     /locus_tag="PKN31_05935"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOA99498.1"
                     /translation="MIQIKTALISVSDKTGLEELATFLQKQGVEIIATGGTARFIRNL
                     GIEIKEVSEITGFPEILGGRVKTLHPVIEGGILARRDNPEDQEDLKKHQIKPLDLVVC
                     NLYPFEKMIEKEETSLEKVVEEIDIGGITLLRASAKNFRYVVSVSSPCQYQELMEEMT
                     KNGGAFPKDKSQKWAIEAFFRTSAYDGHIANYLTRLTYPEEEPFYPQFSLLLRKKYPL
                     RYGENPHQRGAFYEDLSLDPQGFMRYIEHWGEKELSFNNLYDVQAAFSLVREYEDPAV
                     VIVKHNNPCGVATDSNLSVAAQKALSGDPVSAYGGIVAFNRPVDKESAKVFRKLFIEV
                     VIAPSYEEDARKIFSSKKNLRVIQAPLSSPSYFDLKKVDGGFLLQDKDIIDLDKNKLQ
                     VVTEKKPTPQEEEDLLFAWKVAKHVKSNAIVLAKDRETVGVGAGQMSRIDALYIALLK
                     AHGREKGAVLASDAFFPFSDVVEEAGENGITAIIQPGGSIRDEDSIQACNKLGISMVF
                     TGIRHFRH"
BASE COUNT         1844 a         1428 c         1597 g         2031 t
ORIGIN      
        1 gacctaaaat gggtcgacag aaagtagttt tagcttattc aggaggattg gatacttcag
       61 tagctattcg ttggctggaa gagcaattta atgccgaggt ttatgctctt actattgatg
      121 taggccaagg taaagacgta gaagaagtgc gcaaaaaagc tctgagcatt ggagcaaagg
      181 aagcactggt ttttgatgcg cgggaagagt ttttaaatga gtacgttttg cccgctctat
      241 gggctcaggc catttatgaa gatagatatc ctttggctac tgctctttct cgccctttga
      301 ttgccaagta tctggtcaaa gtagcagagg aaaaaggtgc aaccatgata gctcatggtt
      361 gcacgggaaa aggtaacgac caggtgcgca ttgaagtttc agtgatggct ttgaatcccg
      421 atttacaggt ggtggctccc gctcggcaat gggggtggtc aagagaagaa gaaattgaat
      481 acgctcatgc caatggtatc ccggttccca ccactttaga gcaaccttat agtattgatc
      541 agaatatctg gggaaggagc atcgagtgtg gagttttaga agacccgttg gttgagccgc
      601 ctgcagaggt atttgagtgg acgagagacc cgaaagatgc tccttcggaa cccgcttatt
      661 tagaaattgg atttgaggaa ggagtacctc gtcttttggg aggagagtat tactctcttt
      721 tgagtttggt ggaagaatta aataagatag gtggtgaaaa cggctttggt cgagtggacc
      781 atgtggaaaa tcggttagtg ggaataaaat ctcgagaaat ttatgagtgt ccggcagctt
      841 taattctcct tgaagcttac cgtgaccttc aaaatctggt tcttcctcga gaagtaatcc
      901 acttcaagcc ccacctggag gaaaagtatg cacaattggt gtacgatggt ctctggtatt
      961 cacctcttcg ggaagccctg gatgctttta tgaaatcctt tttaccgcgg atgacgggaa
     1021 tagtaagagt taagctttat aaaggtagta tgcaggtagt ggggagaaaa tcggaatatt
     1081 ctctctatga ttggggatta gctacctatg accgaggaga tttgtttgat catcaggcca
     1141 gtgaaggctt tattaaaata tggggtcttc ccgctcgagt gcaggctact ctggagagaa
     1201 ggaaaaaatc atgaccaact tatggggaag tcggatggaa aaaggcttag accaggaagt
     1261 gaaagaattt tccacttcta ttccggaaga tttcttcctc tataagtatg acctgtgggg
     1321 aagtgccgct cactgcaaaa tgttagaaaa agtaggaata atatcggaga aagagagcaa
     1381 agaaattttg cgaggcttac gggtgatttt tcaggagata gaggagggga aggtagatcc
     1441 ttcccctttt gaggacatac atagcctggt agaaatccgc cttcgggaaa tagcaggaga
     1501 gaaaataggc ggtaaacttc ataccgctcg tagccgtaac gatcaggtgg ttttggatga
     1561 gcgcctgttt ctgcgagaga gaataggaga ggttatagaa aaaatcagtt ttcttcagga
     1621 agccttactg cgaaaagctc aagaaaacaa agatgtggtt atgcctggct atactcacct
     1681 tcagccagca caaccggttc tttttgctca tcacctttta gcctattttt ttatgttcca
     1741 gcgggatata gaaagattag aagataccag aaaaagagta aacgtgcttc ctctgggagc
     1801 aggagcgttg tctggaactt cgctccctat cgatagacaa tatgtggctg aacttctggc
     1861 ttttcctcga gtgcaggaaa atagtatgga tgccgtatcc gatcgggatt ttattctgga
     1921 atttttagga ggtctgtcta ttttgtttct tcacctcagt cggtggggag aagaaatagt
     1981 tttgtggagc tctccggcct ttcacttttt agaaattgac gatgctttca caaccggctc
     2041 aagtattatg cctcagaaaa aaaatcccga tgtagcggag ctactacgag gaaaaaccgg
     2101 ggaagtattg agctcctggg ttagtttggc tatcacttta aaagggctcc ctctcaccta
     2161 taatcgagat ttacagcagg ataaaccccc tcttttccgg gccacaaaag agactctttc
     2221 ctctcttcgc atagctgctc gtctcactca gaacatcttt cccgaccgag aaagtatgaa
     2281 aaaagcacta tcggtgggtt ttttgaccgc tactgatttg tgtgaatatt tggtaggaca
     2341 gggagttcct tttcgccggg ctcatgcctg ggtgggagag atggtgaaaa aactatctgc
     2401 aagcggaaaa cacctgcggg atttgaagaa ggaagactgg ggagagtatc aacatctgtt
     2461 ccccgaaaat tgggaggaga taatagatga ggaaaagtca gtgagaagga aaatatcgga
     2521 aggcagcacc tctcctcggg aggtggagag agagataaaa ataggagaag aacttttggc
     2581 tcaatcccga gagcgagttt cctcctggag aaaaatagag caagaaagct gggaaaactt
     2641 gatgggtgga agttgtgttc tctaactttt aagatataca ttaaaagaac tcaaaaaata
     2701 tcagtttgtt cgtttacaat ttgtggatgt ttcgggtaaa gctaaaaatg gggagttccc
     2761 gtttagaagt tgtttgctgc tttagagaga gaagttagtt ctgtggtttg gattgtttat
     2821 tatgttcaat aggtagatgg accagaaaat tttggagagt gataggggga attacactga
     2881 ttatttctct aataaaatac gcagagctgc tatatcagaa ggagtaactc ctgccacccg
     2941 ggcagcttgt cctaaagtgg caggtcttac tttttgtagt ttttcccgtg cttcctgaga
     3001 aattacctga atagaagaga agtcgaagtc agggggaata actttattct ccattttttt
     3061 aaactcttct atttctcttt tttgtcgctc aatgtatcct cgatatttta tttctatctc
     3121 cacttcctca attacctgag gctcgagaaa tggcctttcc ggatcaaaag gagctaaatc
     3181 ccggtagcta atctccggac gagaaagaag agcgctggct tttattgcct cttttaccgg
     3241 atttgttccc ttttgtgtta agagactctg caattgagtg gtgggataaa tagtaaggcg
     3301 agagaggcgc tcgatttctt tctggatttt atctctcttt tcctccaccc ggtgatattc
     3361 tttttcgctt attattcccc gctcataagc atagaaactg agacggaggt cagcgttact
     3421 gtggcgaacc accaggcgaa actctacttt tccagtgcgt aaccgatagg gttcatctac
     3481 tcctttggtt actaagtcat cgatcataac cccgatatag ctttcatctc gccgaagaat
     3541 taaagggggt tgacctttga cgtaacttgc ggcattaatt ccggctatta ttccctgtcc
     3601 tgctgcctct tcgtatcctg aagtcccgtt gatttgtccg gctaaaaaaa ggccgggaat
     3661 ttctcgacac tcgagagatg atttaagctg agtaggtaaa atataatcgt attcaattcc
     3721 atatcctggt cttattatat gagctctctc caggccagtt atagagtgga ctatttctcg
     3781 ctgcacctct tctggtagcg aagtaaaaag accctggacg tagatttcgt tacttttctt
     3841 tccctcaggc tccaaaaaca ggaggtgatt ttctctttcg ggaaagcgat aaactttatc
     3901 ctccagggaa ggacattccc tcactgaaga agtatcggag atggagtaga gaagagggga
     3961 gcgatggagg ttggctttta tgatatcgca agttttttta gaagtacggg taataaacac
     4021 tgatgctcct tcataaacct gaggaacccc ggtaaaggaa aagcacagag gcttatcgtc
     4081 gcttttctgc tctttcaaag aggaaaagtc tatagttcgt cggtccagac gaggaggact
     4141 gcaagtgctg agccggcctg actgcagtcc catattgcgt aaattgcaag ctaaatctat
     4201 cgagggaaat tctccctgtc ttcctgcagg atagctcaac tccccgattt taatcaatcc
     4261 atttaaaaat gtaccggtgc acaaaaccac agcgggagca aaaaaggtgg tttgattttt
     4321 taccttcacc ccttttattt cccctttttc atcttgtaat atttcagttg cttctccttg
     4381 aaaaagatag aggtgaggtg tattttccag gaatttccgc ataattaaac tatacaggtc
     4441 tctctgagtt tgagcacgta gagtttgcac tgccggccct ttcccggtgt taactcttct
     4501 tatatgaatg gtagaaaggt cggtagcttt agccatcagg cctcccaggg catctatctc
     4561 tcttactacg tgtcctttcc ccggtccacc aatggcgggg ttacagggca taagggctaa
     4621 atggtgaatg gaggtagtta gaagaagggt ttttgctccc atccgggctg aagcatgagc
     4681 agcctcgcat ccggcatgac ctcctccaat cacgatgacg tcatagtatt cttccataaa
     4741 ttcttcccgt aaaactaacc tcccggaata ttaatgccgg aaatggcgaa tcccggtaaa
     4801 aaccatactg attcccagtt tattgcaggc ctggatggaa tcttcatctc taatagagcc
     4861 accaggctga ataatagcgg taattccatt ttcccctgct tcttctacta catcagaaaa
     4921 aggaaagaag gcatcggatg ccagcactgc tcccttttct cgcccgtggg ccttaagaag
     4981 agctatatat agagcatcga ttctactcat ctgtcccgct cccactccca ctgtctcgcg
     5041 gtctttggct aacacaatgg cattggattt gacatgtttg gctactttcc aggcaaataa
     5101 taaatcttct tcttcctgag gagtaggttt tttctctgtt actacttgca atttattttt
     5161 atctaaatca attatatcct tatcctggag gagaaaacct ccatctacct tttttaagtc
     5221 aaaataggag ggagaagaca gaggagcttg aataactcgc agattttttt tggaggaaaa
     5281 aatcttccgg gcatcttcct cataagaagg ggcgataacg acctctataa acaattttct
     5341 gaacaccttg gcgctttctt tgtctaccgg acggttgaag gccacaatcc ctccataggc
     5401 cgataccggg tctccagaga gagctttttg agctgctacc gagagattag aatcggttgc
     5461 caccccacag gggttattat gttttactat taccactgct gggtcttcgt attctctgac
     5521 caggctaaaa gcagcctgca catcgtagag attattgaag gaaagctctt tttctcccca
     5581 gtgttctata tatctcataa aaccctgagg gtctaaagac aggtcttcat agaaagctcc
     5641 tcgctggtga ggattttctc catagcgaag agggtatttt ttacgaagta aaagagaaaa
     5701 ttggggataa aaaggctctt cttcagggta ggttagtctg gtaagatagt tggcaatatg
     5761 gccatcgtaa gctgaagtcc ggaagaaagc ctctatggcc cacttctggg atttatcttt
     5821 ggggaaagct cctccgtttt tggtcatctc ttccatcaat tcttgatact ggcaagggga
     5881 agagaccgat accacgtatc ggaaattttt ggccgaggct cgaaggagag taatgcctcc
     5941 tatatcaatt tcttccacta ctttctctaa agaggtttct tccttttcta tcattttttc
     6001 aaaagggtag agattacata ctactaaatc taagggttta atctggtgtt tttttaggtc
     6061 ttcctgatct tctggattgt ccctccgggc taatattcct ccctcgatta ccggatgcag
     6121 agttttcacc cgtcctccca gtatttctgg aaacccggta atctcagaaa cttcttttat
     6181 ctctattccc agattacgga tgaatctggc tgtcccaccg gtggctatta tttccacacc
     6241 ttgtttttgc aaaaaggtag ctaattcttc taaaccggtt ttatcggaga cacttataag
     6301 agcagtcttt atttgaatca atcttttcac ctcctgaatt ctattgctca ttttatccca
     6361 ccgagcaaaa aatgcaaaat tatttctgaa ctcgacttgc gcataaagaa caatggagaa
     6421 agaagcgttc ttcttcggga aagtagcagt gggttccccg tggctctgtg ctggtgggca
     6481 tactggatat tttcctggtt atcatctttt gtgtctggag acactcagga gaggtatttt
     6541 ctcttcctca gactctttat ttttatactt ttagtttctt tgtgtttatg cagcagagtg
     6601 ctgttattgc aaacctcatt catttacgcg gcgtggcaat tcttccccca acggtcgttg
     6661 tgaacctcac ctggcagcct gacaatgttg ggaaaaggta aagagattat aaaactaaga
     6721 taacgatgct cgagaaaaaa tcccgttgtg cgcggtaatc tcttttttct tccccctgaa
     6781 agctcttctt tccttctctc ttcattccca aaattcagtt cggaatccaa acttttcaat
     6841 acttatggac ccctgataga accattcagg ggtgaccccc cgccgtcatt cccgaaatct
//
LOCUS       DAOPSS010000086         6856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_9181_length_6856_cov_8.406411, whole genome shotgun sequence.
ACCESSION   DAOPSS010000086 DAOPSS010000000
VERSION     DAOPSS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9181_length_6856_cov_8.406411"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(411..1649)
                     /locus_tag="PKN31_05940"
     CDS             complement(411..1649)
                     /locus_tag="PKN31_05940"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOA99499.1"
                     /translation="MEKTMTAVKKITFKNGFTLILSPRPDSALVAINLCMNLGNLFES
                     EEERGISALMQETLLKGTQRQSAIDFNRAVENMGATINSSSSYFTGRVVMQGPAINMG
                     QMLKLFFEAIKSPAFQEEEIEKEKTFLLNVLRSLDDDPFQAAMLRFKKAFYGRHPYAF
                     PTMGEEKTLRNLGEEEIWSWYRKVYVPNNMVAAVVGDFDPDEVEKIFHEEMGEIIPKE
                     PLAPYLEKFSPLSLKIVDRKEVRDSWMVVGFKAPGLLEEKERVVFDILNNILGGSMYS
                     RLFLKIREERGLSYQVGSLYVPLRGPSFICAYASFPYVYFDAVVRILWEELRGLSHLE
                     KEEFEEAKNYTRGNFLHCLETVFSLSSLFAFFEKVGLGWEFIFRYEKMLREISVEEAE
                     AVYQRFNGQGESMGGIVPAE"
     gene            complement(1651..2910)
                     /locus_tag="PKN31_05945"
     CDS             complement(1651..2910)
                     /locus_tag="PKN31_05945"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOA99500.1"
                     /translation="MWWLKLKKYAFTYTKKVLYSGLRVVVKRMPSPLVTINFWVPAGV
                     KDEEPEKNGLSHFFEHMVFKGTANYPASLLSRQVQALGGVMNAGTSLDTTDFYLVVPR
                     EYWRKALELEVDLLFHPLFDPQEIEKEKMVVIQEIHLDEDDPGERLIHLLYERVFSGT
                     PYGKPILGREDTVKTFTREDLLEHQKRFYHPTNLTLVVAGDIAEEEVFGEAEKLVGEF
                     RDLENFTFSPFPSLPSPNQQIVSCSMDVHRYYGAIGFLTEGIREDDFYLLRLLAVVLG
                     DGIGSRLNVVLREEKKLVDTIHTVYSYYQKAGIFAIFYTFSQGKKEEIEEEIQKEIVN
                     LISDPPRDEELVRGKNLLRSSLFSVLETTLGTAELLGRSDVTDNIDRVVHHFFNLGSF
                     RSEDLVETVKKYLDFERATFVFIEPGV"
     gene            3137..4324
                     /locus_tag="PKN31_05950"
     CDS             3137..4324
                     /locus_tag="PKN31_05950"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOA99501.1"
                     /translation="MQITERVKKIEPSATLSISARAKALKKEGRDVISFSAGEPDFDT
                     PLVIKEEAKKAIDEGFTKYTPTSGTPELKEAIGKKLEKENGLFYTTQEIIVSCGAKHS
                     LFNAILTLCSPGDEVLLPKPYWVTYLEQIKLAQGKPVFLEPSPSTLTVSIEEIKKALT
                     PHTRLIIINNPSNPTGVVWEEGELEEIARLAKEKDLVIISDEIYEHLTYDGAKVKSIA
                     TFPGMKERTIVINGVSKAFSMTGWRIGYAAGPQDVIEAMGRLQDHSTSNPTSISQRAA
                     WKALQSDSFLVKEMVEKLDERRKLMMQHLSEIPSVTFPTPRGAFYVFANFSPYLGKSF
                     QGKPINNSLELAESLLEEAQIACIPGIAFGMEGYLRFSYATSEENIEKGMKRLKNFIN
                     QMN"
     gene            4337..5572
                     /locus_tag="PKN31_05955"
     CDS             4337..5572
                     /locus_tag="PKN31_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HOA99502.1"
                     /translation="MPKIYILDVTNRDGVQTAHLGLAKIEKTMINFFLNEMGVYQSEF
                     GFPTTRHETNYLNANLELAEMGVLSPIRLSGWLRAIKRDVELAYEMVPGLKYLNLSIS
                     TSDQMIHSKFQEKMSRETVIKSMVEAVETAKACGAQGVGINAEDASRSDIDYLIEFAL
                     AGKEAGAERFRYCDTLGYDSPFTIYERIAKIGEATQMPIELHCHNDLGMVVANSVAGA
                     KAAIDVGCDVFINTTINGIGERAGNADLVSVILAVKYASGMDGEKYQLDERINLKTAW
                     KTCKYASYAFRVPIPITQPGVGANAFAHASGIHADGVLKDRKNYELYDFEELGRGEPE
                     IIETGRQIVIGEYSGIKGFRNVYNQLEIEFKDEEEAREVLELARFANVEKQKPLTSDE
                     LRFIAQYPEQAKKILTLTP"
     gene            complement(5607..6374)
                     /locus_tag="PKN31_05960"
     CDS             complement(5607..6374)
                     /locus_tag="PKN31_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOA99503.1"
                     /translation="MNGRLLKNKLREKKPCIGNWVTFTDPTVTEVLCGAGYDFLVIDT
                     EHAPLGIETVQLHLLATKGEEAVPIVRVPWNDSVYLKRVLDTGAPGVMIPLVRNREET
                     ERAISFCLYPPEGIRGFGPRRAAGYDRRIPEYIEKANEEVLIILQIEHFETIENLEEI
                     LKVERLSAIFIGPWDLSGSLGLLGKINHPRVEGYIDTIIEKAHSANIPVGIAASSKAE
                     DVVGWFKRGVQFVTAGTDYGLLSRGCDNSITSIKKSL"
     gene            complement(6416..>6856)
                     /locus_tag="PKN31_05965"
     CDS             complement(6416..>6856)
                     /locus_tag="PKN31_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA99504.1"
                     /translation="FPDQNLAQKWQFSYVSLEKLLRESDFITLHIPLTQETRGFIGGK
                     ELSLMKRSAYLINTARGGIVDEEALYRALKEGIIAGAALDVFSQEPPGSSPLMDLENV
                     IATPHMASDTKEAIRRMDVVSAENVIRVLRGDPPLSAVNVVEKI"
BASE COUNT         2145 a         1523 c         1272 g         1916 t
ORIGIN      
        1 ggagcttgag tggttaaaga gccaaagtta ctcaatttgg cagacacaat ttcgcccagg
       61 tattaatctc atctgagata ggtctcctca gaagacaaaa gataattgaa agactgtgag
      121 agactttaca gagagactca gtagaactta atctggctta tatcccgact ttagaaaata
      181 tagcccttca ttagtcgctt ttatccagca atacaatagt aaatttggtc gagagccaaa
      241 agaccccccc ggtcaacttt ttttctctgg aggaaaacat aaattagact ttattctccg
      301 ccggaaggaa attcggaagg taggatagaa atgttatatt taaccctcaa gttagggagc
      361 gacacatttt cataaaatat tgacagagag ccaggaatat attgaatttt ttattcagcg
      421 ggaacaatgc ctcccataga ctctccttgt ccattaaacc gctggtaaac agcttctgct
      481 tcttccacag aaatctctct caacattttt tcgtaacgaa aaataaattc ccaacctaag
      541 cccactttct caaaaaaggc aaaaagagag gaaagagaga aaactgtttc taaacaatga
      601 aggaaatttc ctctggtata attcttggct tcttcaaatt cttctttctc gagatgagat
      661 aacccccgaa gctcttccca caagattctt accactgcgt caaaataaac atagggaaaa
      721 ctagcataag cacagataaa agatggaccc cgtagaggaa cataaagaga acctacttga
      781 taactcaaac ctcgctcttc cctgattttc aggaaaagac gagagtacat actccctcct
      841 aaaatattat tcagaatatc aaaaaccacc ctctctttct cttctaataa cccgggagcc
      901 ttaaagccca ccaccatcca gctatctctc acttctttac gatctacaat ttttaaagag
      961 agaggagaaa atttttctag ataaggagca aggggctcct taggtataat ttctcccatc
     1021 tcttcgtgaa aaatcttttc tacttcatcg ggatcaaaat cacccactac tgcagctacc
     1081 atattattag gcacataaac tttcctgtac caggaccaaa tttcttcttc tcctaaattg
     1141 cggagagttt tttcttcgcc catagtggga aaggcataag gatgacgacc gtaaaaagct
     1201 ttcttaaacc gcagcatcgc agcctggaaa ggatcatcat ccagagagcg aaggacattt
     1261 aagagaaagg ttttttcctt ttcaatttcc tcttcctgaa aagccgggct tttaattgct
     1321 tcaaaaaata gttttaacat ttgtcccata ttaatagctg gaccttgcat aaccacccgt
     1381 ccagtaaaat agctagaaga gctatttatg gtggctccca tattctctac tgctcgatta
     1441 aaatctatag cactctggcg ctgagtccct ttcagaagag tctcttgcat taaagcagaa
     1501 atccctcgtt cttcctcgct ttcaaagaga ttacctaaat tcatgcacaa attaattgct
     1561 acaagagcac tatccggtcg aggagaaagt attagtgtaa aaccattttt aaaggttatt
     1621 tttttcactg cagtcattgt cttttccata tcacacccct ggctctataa aaacgaatgt
     1681 agctcgttca aaatcaagat attttttcac tgtttccacc aggtcttcac ttctaaaact
     1741 tcctaaatta aaaaaatgat gaaccacccg gtcgatatta tcggtaacgt cagagcgccc
     1801 caatagctct gctgttccta aggtagtttc cagcacactg aacaaactac tgcgcaacag
     1861 atttttacct cgcaccaatt cttcatcccg ggggggatca gaaattaaat ttactatttc
     1921 cttttgaatt tcctcctcta tttcctcttt ttttccctga gaaaaagtat aaaaaatggc
     1981 aaatatacca gctttctggt aataagaata aacagtgtga atggtgtcta ccaacttttt
     2041 ttcctctcgt aaaactacgt tcaaccgaga accaattcca tcacctaaaa ctactgcgag
     2101 aaggcgcaga agataaaaat catcttctct aattccctca gtcagaaaac ctattgcacc
     2161 gtaatagcgg tgtacatcca tagaacagct tactatctgc tggttaggag aaggaagaga
     2221 gggaaaaggc gagaaagtga agttttccaa atcgcggaac tctcctacta acttctctgc
     2281 ttctccaaaa acctcctcct cagcaatgtc acctgccacc accagggtaa ggttggtagg
     2341 atggtaaaag cgcttctggt gctctaataa atcctcacga gtaaaagtct ttaccgtatc
     2401 ttcccttccc aaaatgggct tcccgtaagg agtaccagaa aatactcttt cataaagaag
     2461 atgaatcaag cgttcccccg ggtcatcttc atctaaatga atttcctgga taaccaccat
     2521 tttctccttt tctatctcct gaggatcaaa aagagggtga aaaagtaaat caacttctaa
     2581 ttccaaagcc tttcgccagt attctcgcgg tactactaaa taaaaatcgg tagtatccag
     2641 ggaagtgcca gcattcataa ctccccctaa agcttgtacc tgacgagaaa gcaaagaagc
     2701 aggataatta gcagtacctt taaaaaccat gtgctcaaaa aaatgagata ggccgttttt
     2761 ttcaggttcc tcatctttca ctcccgctgg aacccaaaaa ttaatagtaa caaggggtga
     2821 aggcattctc ttaactacta ctcgaagtcc agaataaaga acctttttag tataagtaaa
     2881 ggcatacttc ttcaacttta gccaccacac ctttccccta atattctttt ttctctttct
     2941 ctgagaactt taatgcctac cggggggata ccatcttcct tccgtgttcc aaatatttta
     3001 agctaccccc ttgacttttt cttctcacta ctatataaaa aagaataaaa ctctaacttc
     3061 agaaacgcaa atttctcact accgtaagta tatcatccct atttctgaaa gcaattattt
     3121 ttcaggaggt gacattttgc aaataacaga aagagttaaa aaaattgagc cttccgctac
     3181 tctatctatt tctgcccgag ccaaggcttt aaaaaaggaa ggaagagatg taatttcctt
     3241 cagtgctggt gagccagatt ttgacactcc tttagtaatc aaagaggaag ctaagaaagc
     3301 tattgacgaa ggctttacta aatatactcc tacctccgga acgccagagt taaaagaagc
     3361 tattggcaaa aaactggaga aagaaaatgg gcttttttac accactcaag aaataatagt
     3421 ttcctgtggt gccaaacact ctctttttaa tgccattttg accctctgta gccccggaga
     3481 tgaggtactt ttgcccaaac cttactgggt tacttactta gaacagataa aattagctca
     3541 aggaaaacca gtttttcttg agccttcccc atctacactt actgtgagca tcgaagaaat
     3601 taaaaaagct ctcactcctc acactcgtct tattatcata aacaatcctt caaacccgac
     3661 aggggtagta tgggaagagg gagaactaga agaaatagcc cggttagcta aagaaaaaga
     3721 tttagtaata atttctgatg aaatatatga acatctcact tatgatggag caaaagtaaa
     3781 aagtattgca acttttccag gtatgaaaga gagaactata gtaatcaatg gcgtatctaa
     3841 agccttttct atgaccggct ggcgaattgg atatgccgcg gggcctcaag atgtaattga
     3901 agcaatgggg agactccagg atcactctac ttccaatccc acctctataa gccagagggc
     3961 ggcctggaaa gctttacaga gcgattcttt tctggttaaa gaaatggtag aaaagcttga
     4021 tgagcggaga aaacttatga tgcaacatct ttcagaaatt ccctcagtca cttttcccac
     4081 ccctcgggga gctttttacg tttttgccaa tttctctccc tatttaggta aaagcttcca
     4141 ggggaaaccc attaataact ctttagaatt agctgagtca cttttagaag aagctcaaat
     4201 agcctgcatc ccaggaatag cttttgggat ggaaggttac cttcgttttt cctatgctac
     4261 ctcagaagag aacattgaaa aagggatgaa gcgattaaaa aattttatta atcaaatgaa
     4321 ttgaggaggt gtatacatgc ctaaaatcta tattctagat gttaccaatc gtgatggagt
     4381 tcagactgcc cacttaggat tagctaaaat tgaaaaaact atgataaact tttttctcaa
     4441 cgaaatggga gtttatcaga gcgaatttgg cttccccacc actcgtcatg aaactaacta
     4501 cctcaacgcc aatttagaat tggcagaaat gggagtcttg agtcctatta gattaagtgg
     4561 atggttgaga gcaataaaaa gggatgtcga attagcttat gagatggtac caggattgaa
     4621 atacctcaac ctctctatct ctacctctga ccagatgatt catagcaaat tccaggaaaa
     4681 aatgagtcga gagacagtga taaaatccat ggtagaagca gtagaaaccg ctaaggcttg
     4741 cggagcacag ggagtgggaa taaatgccga agatgcctct cgctctgata tagattactt
     4801 aatcgagttt gccttagcag gtaaagaagc aggagcagaa cgttttcggt attgtgacac
     4861 tttaggctat gatagtcctt tcaccattta tgagcgtatt gccaaaattg gggaagcaac
     4921 ccagatgcct attgagctcc actgtcacaa tgacctgggt atggtagtcg ctaattctgt
     4981 cgccggagct aaagcagcaa tagacgtagg atgtgatgtt tttattaata caaccattaa
     5041 cgggatagga gaacgagcag gaaacgctga tttagtttcg gtaattttag ctgtgaaata
     5101 tgccagtggt atggacggag aaaaatatca gctggacgag cgaataaatc tgaaaaccgc
     5161 ctggaaaact tgcaaatatg catcttacgc tttccgcgtc cctatcccca tcacccaacc
     5221 cggcgtaggg gctaatgctt tcgctcatgc ctcaggaatc catgctgatg gagtgttgaa
     5281 agaccgcaaa aactacgagc tttatgactt tgaagagctg ggaagaggag aaccggaaat
     5341 catagaaaca ggaaggcaaa ttgtaatcgg tgaatatagc gggataaagg gattccgcaa
     5401 tgtttacaat caattagaga tagaatttaa agatgaagag gaagcacgag aagtattaga
     5461 attagctcgt tttgctaacg tagaaaaaca aaaacccctg acttcagacg agctgcgatt
     5521 tattgctcaa tatcccgagc aggctaaaaa aatcctcact ttgactcctt aaaggaaggg
     5581 tggtttaccc acccttcccc agatatttac agtgatttct ttatagaagt tattgagtta
     5641 tcgcagcctc gagaaagaag cccgtagtct gtccccgcag taacaaattg cactcctctt
     5701 ttgaaccacc ccaccacatc ttctgcttta gaagaagcag caatccctac tggtatatta
     5761 gcactatgag ctttttcaat aatggtgtca atatatcctt ccactcgagg gtgattaatc
     5821 tttcctaata agcctaaaga accagataaa tcccaggggc caataaaaat cgcgctcaac
     5881 ctttctacct tgagaatttc ttccaaattt tctatcgttt caaaatgctc aatttgcagg
     5941 atgattaaaa cttcctcatt ggccttttca atgtattcag gtatccggcg gtcgtagcca
     6001 gctgcccgac gcgggccaaa tcctcgaatc ccctctggag ggtaaagaca aaaagaaatt
     6061 gctctttctg tctcttctct attgcgcacc agaggaatca tgactccagg tgctcctgta
     6121 tccaaaaccc gtttcagata aactgagtca ttccatggca ctcgcactat gggcacagcc
     6181 tcttcccctt tggtagctaa aagatgaagt tgcactgtct caattcccag aggagcgtgc
     6241 tcggtatcaa tgactaaaaa gtcatagcct gccccacata gaacctcagt aaccgtggga
     6301 tcggtaaaag tcacccaatt tccaatacag ggcttcttct ccctaagttt atttttcaac
     6361 agtcttccat tcataaacaa acctccttgc ttttgcattt ttcagaagtt caatattata
     6421 ttttttccac tacattgact gctgaaagag gaggatctcc ccgaagtacc cgaatgacat
     6481 tctctgctga cactacatcc atacggcgta tggcttcttt ggtatcagag gccatatgag
     6541 gagtagcgat aacattttct agatccatga gagggctact acccggtggt tcctgagaaa
     6601 aaacatctaa agcagctcca gcaattatcc cttctttcag tgctcgataa agtgcttcct
     6661 cgtctacaat cccccctcga gcagtattta taagataggc acttctcttc attagagaaa
     6721 gttctttccc tcctataaaa cccctggttt cctgagtaag aggaatatgc agagtaataa
     6781 aatcgctttc ccgtagcaat ttttccaatg atacgtagga aaattgccac ttttgagcta
     6841 agttttggtc ggggaa
//
LOCUS       DAOPSS010000087         6799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_9275_length_6799_cov_8.540480, whole genome shotgun sequence.
ACCESSION   DAOPSS010000087 DAOPSS010000000
VERSION     DAOPSS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9275_length_6799_cov_8.540480"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..1856)
                     /locus_tag="PKN31_05970"
     CDS             complement(363..1856)
                     /locus_tag="PKN31_05970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOA99505.1"
                     /translation="MSEKNYLVFDFGASNGRAIVASFDGEKATTDVVHRFEHGPVYVT
                     GTLYWDILYLFSELMNGLQASLSQYPDIRSLAIDTWGCDFGFIDENGKLVANPVTYRD
                     KNRISRSSLLYEVIPQRELFELSAGATSEIMGIFQLFSFQYDNSLEIRSGRRMLMIPD
                     LLNYFLTGRIYNEYTNATMTLLCDQYNRTWERRILDRMGISPSILNELIMPGDKIGVI
                     QEDIREQFGVGPLSVVAPATHDTASAVAGIPVTDSRKHWAFISLGTWAIMGLETLKPV
                     VTDAVFASGYGNNAIPEGRNMLVKYIAALWIIQQCRSRWRNEYQRNLSWDEIVTASEN
                     AGPSKAYIDVDDPLFGQPQVDMPGIIQEYCQQKGQEIPNSMGEVARCFYESLVLKFRY
                     NLELLEKISNKSLELLHIVGGGSQNKTLCQWIADATGISVVAGPAETTSMGNLIMQLK
                     ADGEIRNLEEGREIVLRSSSIEQYEPGDKGYWDDVYEKYLRLFSFSS"
     gene            complement(2273..2842)
                     /locus_tag="PKN31_05975"
     CDS             complement(2273..2842)
                     /locus_tag="PKN31_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOA99506.1"
                     /translation="MNLSFDQATEVVLKEAHALLATLDADKVKKFIAHVEKDWPEKRL
                     FFWARGRSFLVLRGFAMRLMHMGYEVHIVGEVDCPAIGEGDVLIVASGSGGTSSVLLM
                     VEKAHQYGAEVVAIVGRPGSPLQEKADEVVEFNPEKAGDSVQLYSNGGGTRFEHSLML
                     FFDSIVLSLVSPRREEAYREMMKRHANLE"
     gene            complement(2826..3479)
                     /locus_tag="PKN31_05980"
     CDS             complement(2826..3479)
                     /locus_tag="PKN31_05980"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOA99507.1"
                     /translation="MKPRFQLALDIVTREKAKQLVSSLLDYVDIIEVGTPLILRYGLS
                     IVTEIKSLVGEKKVFADTKIVDAGGWETEESLRSGADMVSVLAGASFFTLQEAKEVCQ
                     ERGAQLVVDTIDFFFPCPEKMKEIREVNPDFLSLHLASDAFQKGESWLEVMEKSPFLR
                     EGGYSFMVAGGISAVSLPQIMKEVSPAVVVVGSAITNSPHPEEEAREIRRIIDEFEF"
     gene            complement(3483..4778)
                     /locus_tag="PKN31_05985"
     CDS             complement(3483..4778)
                     /locus_tag="PKN31_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOA99508.1"
                     /translation="MAVVKPFRGYFYSSEFIKNHPIDRLAAPPYDVISEKEKARLAEE
                     PYNFVHLTLGKVENGYQKAKELLQSWIREGVIEQDETPSFYIDEQEFQLNSSGRKKRT
                     GFVALVRLESWEKKIIMPHERTMPKYSMDRLELLRATRANLEQIFGIFNDSSGGIDEL
                     LEENKKEENFMFQFTDYQGVTHRLWRIKEKEVIDKIKRILSPRTIIIADGHHRYETSL
                     MYREERRKELQDPWEEIPEDYVMMTLVNMKNPGLLILPTHRLVHDLPEERVRGFFDRC
                     QSHFIVNNFSNEREMEEFLRTAPPHTIGVYDKVEDKWGAITLKDLKVMDEKLGKDNVN
                     RYLDTTILHEVVFKEILGINKEGKGESEYVDYLRGTKDVFALAREENHYQLVFVMRST
                     PLERVEEAVAYAQRMPQKSTYFYPKVWSGLVFRLLDEEE"
     gene            complement(4801..6576)
                     /locus_tag="PKN31_05990"
     CDS             complement(4801..6576)
                     /locus_tag="PKN31_05990"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOA99509.1"
                     /translation="MPFIKVPLLNRVWEVKRGENLLEALRREGIEVDSYCGGMGSCGK
                     CVVKVKGEEISSPNSLEEKHLKEKIKEGFRLACQVEVYGEVEVDVSPSLTSYGEVMVK
                     TQEARREELPVCQAEVNVTKPPIDGVSSLQEILSSQLQGKGKKWSLASLYHLSHLGEE
                     RETEFEVITDEEKIRYVNKLSSDKSFLGMAFDLGTTTVGGELVDLTTGTSLHRLGALN
                     RQTQFGADVISRLRAIQDDPYSLHRLQELLVQTMNDLIQEACEKVNKNPERIFAVTVA
                     GNTIMEHLFLGVSPLSIGVAPYTPVFREEQVLDARELKIVANPEAQVYLFPSLAGYVG
                     GDILSGVVAFDLIERKGLTLYVDIGTNGEIVLIDGENIFCCGTAAGPAFEGAQIKHGT
                     RATLGAISAVEIKDGDIKIYTIGDLAPRGICGTGLIDALACFLREGLLSPTGRFKEND
                     KWSNRLIKEGGSQAFLLSYDPPIFITQEDISQLQLAKAAIQAGREILLKEAGRKEEEI
                     EEVILAGAFGSFINPESAKTIGLIPQGAKTTSVGNASLYGAKKALLSRDFRRKVKVLF
                     QKARYIELSARNDFSDYFYQSLIFN"
BASE COUNT         1931 a         1698 c         1203 g         1967 t
ORIGIN      
        1 caaagaccaa atgacggtcg gtccaccata ccctttactt ttataccata ttaaaccagc
       61 taaagtaatc atactaatga cggcttgggg aattgctgct tttttctgtc gctgcaataa
      121 ttctcctcat ccgtcacccc ggcaattttt aagccggggt ccagactcct tatgacctct
      181 tctttttttg tcatcgcgag ggtcgcaaaa acagacagcc tatggcgtaa gattaaaaag
      241 cacaatagat gaaaaagatt tggaataatt ccgttcgaga gaaagaaagt tacatgggtt
      301 aaacatggtt tttatagata atgccctcga caatgaatca gcccatcaaa cagatgagcc
      361 ctttatgagc taaaagaaaa gaggcggaga tacttttcgt agacgtcatc ccagtaaccc
      421 ttatcacctg gctcatattg ctcaatggat gatgagcgaa gcacaatctc cctgccctct
      481 tctaagtttc tgatctcgcc atcggctttt agctgcatta tcaaatttcc cattgatgtg
      541 gtttccgccg gtccggcaac cactgaaatt cctgtggcat cagcaatcca ctgacagaga
      601 gttttattct gcgaccctcc acccacgata tgaagcaact cgaggctctt attgcttatc
      661 ttctccagta actccaggtt atagcgaaat ttcaaaacca gactttcata aaaacatcgg
      721 gcaacttcac ccatagagtt tgggatttct tgtccttttt gttgacagta ttcctggata
      781 atcccgggca tatccacctg aggttgacca aacagggggt catccacatc aatgtaggct
      841 ttagacggtc cagcattttc tgaagcagtg acgatttcgt cccagcttaa gttcctctgg
      901 tattcatttc tccaccgact tcgacactgc tggatgatcc acaaagcagc gatatatttt
      961 accaacatat tcctaccttc cgggatagcg ttgtttccat agcccgatgc aaaaactgca
     1021 tcagttacaa caggctttag agtttccaaa cccataatag cccaggttcc caaactgatg
     1081 aacgcccaat gttttcgaga atcagttact ggaataccag ccacagctga agcagtatca
     1141 tgagttgcag gagcaacaac ggaaagcggg ccaactccaa attgttccct gatatcctcc
     1201 tgtatcactc caatcttatc accaggcata atcagctcat tgagaatgga gggagatatt
     1261 cccatacggt cgagaattct cctctcccag gtgcggttat attggtcaca taaaagggtc
     1321 atggtagcat tggtgtactc gttataaatt ctcccggtta aaaaataatt taagagatca
     1381 ggtatcataa gcattctccg accacttctt atttccagag agttgtcata ctggaaagaa
     1441 aaaagctgga aaattcccat gatttcgctg gttgcacccg ctgataattc aaacaattct
     1501 cgctgaggaa tgacttcgta taatagcgag gaccgactta ttcggttttt atctcgatag
     1561 gtcaccgggt tagcaaccaa tttcccattt tcatcaataa aaccaaaatc gcaaccccac
     1621 gtatcaatgg ccagagatcg aatatctgga tactgtgaca gggaagcctg aagaccattc
     1681 ataagctcag aaaacagata aagaatatcc cagtaaagag tcccggtaac atataccggg
     1741 ccatgttcaa accgatgaac tacatcggtg gttgctttct ccccatcgaa gctagcaaca
     1801 attgctcttc cgttactggc accaaaatca aacaccagat aatttttttc agacatttgc
     1861 tcctcctcca gctctatagt tttaaccccc tcttcccatc taaatctttc ccccccatca
     1921 aataccaaca aagacgataa aaaaaatact taacttcttg aacttactat catttgctta
     1981 actattatca tctttcatta tattatagcc ttctgcataa aatacatgga ttgtttttct
     2041 acaaagaaat cgggtaaaaa atttaaaatg agtgaaaatt gaattcaatc ttcactctgg
     2101 caactaatat gcaagaatca cattttctgg aggttaagtt cagaaaatgg gcaagagctg
     2161 atcctcatga tgaaaagact ctttcggctc taagcgagtt tttggagggg cacttcatga
     2221 tcacccgtat tcatgagcat attctaatca gtcatcaaaa actgaaatag cgctactcca
     2281 gattagcatg gcgcttcatc atctctcgat aagcttcttc ccgccggggg gaaaccaaac
     2341 tcaagacgat gctatcaaaa aagagcatca aagaatgttc aaaccgggtc ccacctccat
     2401 tagaataaag ctgaacgcta tctcccgctt tctccgggtt aaactccact acctcatcag
     2461 ctttttcttg aagaggagaa ccaggtcttc ccactatagc caccacctct gctccatact
     2521 ggtgggcttt ttctaccatg agcaaaaccg atgatgtccc accagaaccc gaagccacta
     2581 ttaaaacatc tccttctcct atggccgggc aatccacttc tcctacaatg tgcacttcat
     2641 agcccatgtg cataagacgc atagcaaagc cccgcaacac caaaaagctt cttcctcgcg
     2701 cccagaaaaa aagccttttc tcaggccagt ccttttccac atgagcaata aattttttga
     2761 ctttatcggc atccaaagta gcaagaagag catgagcctc tttaaggact acctccgtcg
     2821 cctggtcaaa actcaaattc atcgattatc ctccttatct ctcgagcttc ttcttcagga
     2881 tgtggagaat tagtaatggc tgagcctacc actaccacag cgggagaaac ctctttcatt
     2941 atttgcggca gagaaacagc gcttattcct cctgctacca tgaaggagta acccccttct
     3001 cgaagaaaag gagatttttc catcacctct aaccagcttt cccctttctg aaaagcatca
     3061 gaagcaaggt ggagagaaag aaaatcggga ttaacttccc ttatttcttt cattttttca
     3121 ggacagggga agaaaaaatc aatggtatcc accaccaact gcgctcctct ttcctggcaa
     3181 acttcttttg cctcctgcag agtgaaaaaa gaagcaccag ccagcaccga aaccatatct
     3241 gctccacttc tcagagattc ctctgtctcc cagcctccag catctacaat tttagtatca
     3301 gcaaaaactt ttttctctcc cactaaactt tttatttctg taactataga aagcccgtaa
     3361 cgaagaatta gaggggttcc cacctctatg atgtctacat aatctaaaag ggaagagaca
     3421 agctgtttag ctttttcccg ggtcacaata tccagagcca gttgaaagcg gggtttcata
     3481 gcttactcct cctcatccag caaacgaaaa accagcccgc tccacacctt ggggtaaaag
     3541 taggtggatt tctggggcat tcgctgggca taagcaaccg cttcttccac tctctctaag
     3601 ggagtggagc gcataacaaa gaccagctgg tagtgatttt cctcccgagc taaagcaaaa
     3661 acatctttag tcccccggag ataatcaaca tattcgctct ctcctttgcc ttctttgtta
     3721 attcctaaaa tttctttaaa caccacttca tgtaaaatag tcgtatctaa atagcgattc
     3781 acattatctt ttcccagctt ttcgtccatc acttttaagt cctttagagt tatagctccc
     3841 cacttatctt ccaccttatc ataaacccca atggtgtgag gaggagcagt acgcaaaaac
     3901 tcttccattt cccgctcgtt agagaaatta ttaactataa aatgactctg gcaccgatca
     3961 aaaaaccctc tcactctttc ctcgggcaaa tcgtgcacta atcgatgagt gggaaggatt
     4021 aaaagtccgg ggtttttcat gttgactaaa gtcatcatca cataatcttc tggtatctct
     4081 tcccaggggt cttgcagctc tttccttcgc tcttctcgat acataaggct ggtctcatag
     4141 cgatgatgac catcagcaat gataatggtg cgaggactca gtattctttt tattttatct
     4201 attacttctt tttcctttat cctccacaat cggtgcgtaa ccccctggta atcagtaaac
     4261 tgaaacataa agttttcttc ttttttattt tcttctaaaa gttcatcgat tcctcctgaa
     4321 gaatcattaa aaatcccgaa tatctgctcc aaattggctc gagtagctcg caaaagttct
     4381 aatcgatcca tagaatattt aggcatagtc cgctcgtgag gcattattat cttcttttcc
     4441 cagctttcta aacgtaccag agctacgaaa cccgtccttt tttttcgccc cgaggaattg
     4501 agctgaaatt cctgttcgtc tatatagaaa gagggagtct catcctgttc tattactcct
     4561 tcccttatcc aggattgaag cagctctttt gctttttggt agccattctc tacctttccc
     4621 agagtgaggt ggacaaaatt ataaggctct tcagctaacc gggctttttc cttctccgat
     4681 attacatcat aaggaggagc tgccagacga tcaatgggat gatttttaat aaattctgaa
     4741 gaataaaaat aacctcgaaa cggtttaact actgccatct tccctatctc cttttccagt
     4801 ctaattaaaa atcaaagatt gataaaaata atcagaaaaa tcatttcttg ctgaaagttc
     4861 aatataacgg gctttctgga atagcacttt aacctttcgg cgaaaatccc gagagagaag
     4921 agcttttttg gctccataga gagaagcatt gcctactgaa gtagtcttcg ctccctgagg
     4981 aataagacca attgtcttag cgctttccgg gttaataaag ctaccaaaag ctcccgcaag
     5041 tattacttct tctatctctt cctcttttct tcctgcctct ttcaagagaa tttccctgcc
     5101 ggcctgaatg gctgcttttg ccagttgtag ctgagaaata tcttcctgag taataaaaat
     5161 aggggggtca tagctcaaaa gaaatgcctg gctacctcct tcttttatca gtcgattgct
     5221 ccatttatca ttctctttga accttccggt aggactcaac agcccttccc tcaagaaaca
     5281 agctaaagca tcgatcaggc cagtgccgca tattccccga ggagctaaat ctccaatggt
     5341 gtaaatcttt atatctccat cttttatctc taccgccgat attgctccca gggtagctcg
     5401 agtcccgtgt ttgatctgtg ctccttcaaa agcaggaccg gctgccgttc cacaacaaaa
     5461 aatattttct ccatctatca aaacaatttc tccattagtc cctatatcta cataaagagt
     5521 taaacctttt ctttctatca aatcaaaggc taccaccccg gatagaatat cccctcccac
     5581 ataaccagcc agagagggga ataaataaac ctgagcttcc ggattggcca ctattttaag
     5641 ttcccgggcg tcaagcacct gttcttctcg aaaaactgga gtgtaaggag ctactccaat
     5701 agaaagagga gaaaccccca gaaagaggtg ctccattatg gtatttccgg ctacagtaac
     5761 agcaaaaatc ctttccgggt tcttgttcac tttttcgcag gcttcctgga ttaaatcgtt
     5821 catagtttgt accagaagtt cctgtaaacg atggagacta taggggtcat cttggatagc
     5881 ccggaggcgg gatatgacat ctgctccaaa ttgagtctgt ctatttaaag ctcccagacg
     5941 atgaagagaa gtgccagtgg tgaggtctac cagctctccc cccacagtag tggtacccag
     6001 gtcaaaagcc atccctaaaa aagatttatc agaagaaagt ttattaacat accggatttt
     6061 ttcttcatca gttattactt caaattctgt ctctctttct tccccgaggt gagaaagatg
     6121 ataaagagag gctaaagacc atttcttacc ctttccttgc agctgagaag acaggatttc
     6181 ttgaagagaa gacactccat ctatgggagg ttttgtcaca tttacctctg cctggcagac
     6241 aggaagttct tcccgccggg cttcctgagt ttttaccatc acttctccat aagaggtaag
     6301 agaaggagaa acatctacct ctacttctcc atatacttcc acctgacagg ccagacgaaa
     6361 tccctctttg attttttctt tgagatgttt ttcctctaag gaattaggag aagatatctc
     6421 ctctccttta acttttacca cacattttcc gcaagacccc atacctccac agtaggaatc
     6481 tacctctatt ccttcccgcc gcagggcttc caacagattc tctcctcttt ttacttccca
     6541 aactcgattc agaaggggaa cttttatgaa tggcatttta gtatttatct atcctcttct
     6601 ctcttaactc atcaagaata ccttttcctt tacatcaagt tagtttacca tttttgtcaa
     6661 gaacaaccta cggcaaagcg tagcagcttg caacactctg cctcttttta tcatagtaag
     6721 ggtcgcattt acgtttcctg tggtgatctt actttgaatt gcgtatacac agccatcaca
     6781 acaattaggt agaaaaaga
//
LOCUS       DAOPSS010000088         6422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_9936_length_6422_cov_6.467881, whole genome shotgun sequence.
ACCESSION   DAOPSS010000088 DAOPSS010000000
VERSION     DAOPSS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9936_length_6422_cov_6.467881"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            636..1367
                     /gene="rsmG"
                     /locus_tag="PKN31_05995"
     CDS             636..1367
                     /gene="rsmG"
                     /locus_tag="PKN31_05995"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOA99510.1"
                     /translation="MDNLGKRRGILERGLEELGLSLEKSRIEQMLEYGEMLYFWNQRF
                     NLTGFKTPEEILVDAILDSLTVFSTPLFEEKIERLVDVGSGGGVPAIPIKIFYPRIHL
                     TLVESNAKKCRFLEEVVRELSLDGARVARGRGEELAHEEAFRESFDLSTARALGSLAT
                     NLELTVPFLKTGGVALYYKGKEVKEEIEKSKKALSLLRAEVEKIWEVAVPFRERKNFL
                     VAVRKKDAAPPSFPRRAGIPQKRPL"
     gene            1987..2784
                     /locus_tag="PKN31_06000"
     CDS             1987..2784
                     /locus_tag="PKN31_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOA99511.1"
                     /translation="MPIKEGERVYLRLEDGKNYLLKVEKGKFFSTHLGNIYFDHIIGK
                     EFGEEVYTSQGKKVYLLQPGIVENIFHMKRRTQIVYPKDLGFILLMLDIKENDRVIDV
                     GLGSGAMCGALARLVGERGKVYAYEKREEFIAIARHNLSQWGWEDRVEIKQRDIKDGF
                     EEKDVDALFLDVPNPWDYLKECWRALKGGGRLAIVSPTAGQVMEVLRGLEALPFEDTE
                     VWENLFRQYKTNPSAFRPVDRMVAHTTYMIFSRKVSPPDWEVDLKET"
     gene            2809..3570
                     /locus_tag="PKN31_06005"
     CDS             2809..3570
                     /locus_tag="PKN31_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA99512.1"
                     /translation="MGIIVAVANQKGGVGKTTTCVNLGACLAEKGKKVLLVDIDPQGN
                     ATSGLGIDRRQLKQCIYDLLINGEKARGLVLETGISRLWLLPATIRLAGAEIELVPLM
                     GRESRLKEGLREVVSDFDFIFIDCPPSLGLLTVNALTSAHRVIVPIQCEYYALEGLGQ
                     LMNTFHLVHNFLNPQLELWGVLLTMFDSRTNLSQQVVEETRRVLGDKVFRTIIPRNVR
                     LSEAPSFGKPIIMHDASSIGAAAYRELAEEVLENE"
     gene            3563..4393
                     /locus_tag="PKN31_06010"
     CDS             3563..4393
                     /locus_tag="PKN31_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOA99513.1"
                     /translation="MSKRGLGKGLDALIPGAGAFGARAIQEIDINSIKVNPLQPRTQM
                     DEEGLEDLANSIRVHGVLQPLLVRKAEEGFELIAGERRWRAARRAGLSTVPVIVEEIE
                     EKSKLEIALVENLQREDLPPLDLARGIKKLMEDFNLTQEEVARRVGKKRSTIANLLRL
                     FDLPPSIQDLLQEGKITLGHAKALLSAESQERQEELVEEILTHSLSVREVEKRVKKAA
                     PQEEPAGHSREEKNLQELLTHYLSARVRVGRGKITIEYHGQEDLERLYSLIVGKEGTF
                     "
     gene            4506..5570
                     /locus_tag="PKN31_06015"
     CDS             4506..5570
                     /locus_tag="PKN31_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOA99514.1"
                     /translation="MLDKKEEEQFTIFWLHPTKGQLKRFHFSWSTLLLGALVLVIGVG
                     LGIGGVLWYQSRIKKELCLALEKMEETQKSAAVLEAQKKEQEEKLKQLTQETEAVIKE
                     LESLRELDEKVRNVLEKDLQSRFQKLGIDLSLSSVSVPSYIPLNRFLGELHVAPLGVG
                     GPERLSSFSLVRDPSPPSSSDHDSDFGRQAKALEDNLSWMRTETLARKKSLEEVVEVA
                     QQRDRLLYVVPMRWPTWGRVSSNYGWRNDPFTGRRAWHTGVDIAAPTGRNVVATAEGK
                     VIFTGWNGNYGKCVIVRHQFGYETVYGHLSKILVNTGDAVKKEQIIGQVGSTGRSTGP
                     HLHYEVRRYSEVINPWPHLP"
     gene            5590..6075
                     /locus_tag="PKN31_06020"
     CDS             5590..6075
                     /locus_tag="PKN31_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99515.1"
                     /translation="MKQNSIIGIVIIFLLCFSWPVWGQVGKVVITDVSVQELAGDVSI
                     SIRGEIQAEGEGSIFLQASTTYENKEYFTAPREIKYALSREAFSLSFSREDFELVDLK
                     ENKVEKLPQLAKGERLVVYVYEKKITSPEGQTEEVKQDIERTGYALRGILAKATRVLE
                     F"
BASE COUNT         1838 a         1266 c         1670 g         1648 t
ORIGIN      
        1 cctatgatta ccaatagaag gacgaaaaga agaaacaaaa acgtggattc ccgattgaag
       61 atttcgggaa tgacaaaaaa ggggtcattc ctgaatgtct ctatcaggaa tctaaataat
      121 aggacaggaa accctctatg tattttttcc tcttcctcgg taaaaacatt tggattcccg
      181 actaaatatt tcaggtacga cgaaagaggc gtcactcctg aacgtctcta cgagaggtcc
      241 atatacagta ttcctaaagc ctcttaaatt gctcaaacct tataaaagta agctacatta
      301 tgttaaaaaa ttcgggatta ccccactcag gagtatgcga gaatcatcgt agccttcggg
      361 gtgatctttg gtaatccaac aaaaacgccg ccacagagcc ttcgcctttt gcgatgatat
      421 catgaaggaa acatcccttc ttcctctttt ttctcataga gaggatggta taatgagaaa
      481 actccaggga agggattcta atctttctgg aaagcgtctg tggataacct ggggattaag
      541 ggtcgggcaa agaaggagtg atagaaatcg ctctattaaa gaatgtgccg tttgtagaca
      601 attcacagag ggtggaaaaa aggggaaaat aatctgtgga taacttgggg aaaagaaggg
      661 gaattttaga gaggggactg gaagaattag gcctctctct tgaaaagagc cggatagaac
      721 aaatgcttga gtatggggag atgctctatt tctggaatca acgcttcaac ttaacgggct
      781 tcaaaacccc cgaagaaata ctggtagacg ccattttaga ttctctcact gttttttcca
      841 ccccgctttt tgaagaaaag atagaacgat tagtggatgt ggggagcggg ggaggggtgc
      901 cagccattcc catcaagatt ttttaccccc ggatacatct cactttagta gagtctaatg
      961 ctaaaaaatg tcgtttctta gaagaagtgg tgagggagct atctcttgac ggtgctcggg
     1021 tggcgagagg aagaggagaa gagcttgctc atgaggaggc ctttcgagaa agctttgatt
     1081 tgtctacagc ccgagctctg ggctctttgg ctacaaattt agagctgacc gtgccttttt
     1141 taaaaactgg tggagtggct ctttattata aaggtaaaga agtaaaagaa gagatagaaa
     1201 agagcaaaaa agccctctct cttcttcgtg ctgaagtgga aaaaatatgg gaagtagcgg
     1261 ttccctttcg agagagaaaa aacttcttgg tggcggtgag gaaaaaggat gccgctcccc
     1321 cctccttccc ccgcagagcg ggcattccgc aaaagaggcc attataaagg aaagaggcaa
     1381 tcctctattg ttttgcaccg cctccggagg catcaagagg cccatcataa acttcgcata
     1441 gcagcttgcc ggcatgagaa caagcaggaa agattgaaaa aaccaggata acaacgctgg
     1501 aaaaaactcc agcatgcgcg ctaatcctga ctggccataa taagcatcat ttagtcctct
     1561 cgtctcaaat agtcatcgcg agggtcgtgg tttcgcttct gtggcgatct ctgagttttt
     1621 gacataatgt agctcacttt ttaaaatgac ggaagatgct cccttatagc ccgtcgttgt
     1681 gcccgagatc gttggttggg aatccaccac tctctgtcat tccgatagag acactcagga
     1741 atgacatctg aggagggtca ctgtggcgac agaaaacgct ccactaggag tcttttgcct
     1801 tttctgttta tatgttttta tcttacgtcg taagccgtct gcttttcctt cccccccgga
     1861 caaaattgtt ccacgtggaa caattttgtt tcccctctcc ttgaatccga gcttatgtgt
     1921 tacaattgga agcagtttgt ttcggtattt tagaagtcaa gagtttctcg gattcaagga
     1981 gagaatatgc ctattaaaga aggagaaaga gtatatctgc gattagagga cgggaaaaat
     2041 tatcttctca aggtggagaa ggggaaattt ttcagtactc atctgggaaa tatctatttt
     2101 gaccacataa taggaaaaga gtttggagaa gaagtttaca ccagtcaagg gaaaaaggtt
     2161 tatctcttac agcctggaat agtggaaaac atcttccata tgaaaaggag aacccagatt
     2221 gtttacccca aagatctggg ttttattctc cttatgctgg acataaagga aaacgatcgg
     2281 gtaattgatg tgggtttagg aagcggggct atgtgtggag cactggcccg cctggtggga
     2341 gagaggggga aagtctatgc ctatgaaaaa agagaagaat tcatcgccat agcccgccac
     2401 aatctttctc aatgggggtg ggaagacaga gtggaaataa aacaaagaga tataaaagat
     2461 ggttttgaag aaaaggacgt ggatgctctt tttctggacg ttcccaaccc ctgggattat
     2521 ctcaaggagt gctggagggc cctcaaggga ggaggaaggt tggccatagt ttcacccact
     2581 gccggtcagg ttatggaggt gttgagaggg ctggaggctc ttcccttcga ggataccgag
     2641 gtgtgggaaa acctcttccg acaatataaa accaatccct ctgcttttcg tccggtagat
     2701 aggatggtgg ctcacaccac ttatatgatt ttttctcgaa aagtttcacc tcctgattgg
     2761 gaggttgatt tgaaagaaac ataaaattag tttagaatat acccatctat gggtatcata
     2821 gtggcagtag ctaatcaaaa agggggagtg ggtaagacca ctacctgtgt aaacttggga
     2881 gcttgccttg cagaaaaggg aaagaaagtg ctgcttgtgg acatcgatcc tcagggcaat
     2941 gctacgagcg ggctggggat agaccgtcgg cagctaaagc agtgcatcta cgacctttta
     3001 atcaacggag aaaaggcgag gggattggtg ctggagaccg ggatttctcg tctctggctt
     3061 ctccctgcta ctattcgcct tgcgggggca gaaattgagc tggttcccct gatggggaga
     3121 gaaagccgcc tgaaggaggg gttaagagaa gtggtttctg attttgattt tatttttatc
     3181 gattgtcctc cttctctggg tcttttaacc gtcaacgccc tgacctcagc tcatagagtt
     3241 attgtgccaa ttcaatgtga atattatgct ctggagggtc tcgggcaatt gatgaacact
     3301 tttcatctgg tgcataattt tttgaatccc cagctggagc tgtggggggt gttgttgacc
     3361 atgtttgatt ctcgaaccaa cctctctcaa caggtggttg aagaaacgag aagggttctg
     3421 ggagataagg tttttcgcac tattattcct cgcaatgttc gcctgagtga agctcccagc
     3481 tttggaaaac caataattat gcacgatgcc agctccatag gggcggcagc ctatcgggaa
     3541 ttagctgagg aagtgctgga aaatgagtaa gcgaggactg ggtaagggct tagatgcact
     3601 gatcccggga gcgggggcct ttggggctcg cgctattcaa gaaatagata taaatagtat
     3661 aaaggttaac cctcttcagc ctcgtactca gatggatgag gaggggctgg aagacctggc
     3721 taactccatc cgggtgcacg gagtccttca gccactcttg gtgagaaagg cagaagaggg
     3781 gtttgagcta atagctggag agagaagatg gagagcggca aggcgggcgg gcttatccac
     3841 ggttccggtg atagtagaag agatagagga aaaaagtaaa ttggaaatag ctctggtaga
     3901 aaacctccag cgagaagacc tccctcctct ggacctggcc cgggggatta aaaagttaat
     3961 ggaggacttt aatctaaccc aggaagaagt ggcaagaaga gtggggaaaa agcgctctac
     4021 cattgccaat ctgcttcgtc tctttgattt gcctccttca attcaggacc ttttgcagga
     4081 agggaaaata actttaggtc atgccaaggc ccttttgagc gcagaatcac aggaaagaca
     4141 ggaggagctg gtggaggaaa ttctcaccca ttctctttct gttcgagaag tagaaaagag
     4201 agtaaaaaaa gccgctcctc aggaagagcc tgctgggcac tccagagaag agaaaaacct
     4261 tcaagagctt ttgactcatt acctttccgc tcgagttaga gtgggcaggg ggaaaattac
     4321 catcgaatac catggccagg aagatctgga gcgcctttat tctctgattg tgggaaaaga
     4381 aggaactttt tgaaggataa gcttgaatat tttttttgtt ttatgataaa attcaggtta
     4441 atagtttgaa aagcaggaaa taacgagcac cagttggtaa aataaaactt tcaggaggat
     4501 cgtaaatgct ggacaaaaaa gaagaggagc agtttacgat tttctggctt caccccacca
     4561 aggggcagct taaaagattt catttttcct ggtcaacgct ccttttggga gctctggttt
     4621 tggtgatagg tgttggtttg ggtatcggtg gagtgttgtg gtatcaatcg aggattaaaa
     4681 aagaactttg tcttgctctg gaaaagatgg aagaaaccca gaaaagcgct gctgttttgg
     4741 aggcccagaa aaaagagcaa gaagaaaaac tgaaacagct aacccaggag accgaagcag
     4801 taataaaaga actggaaagc ttgcgagagc tggacgaaaa ggtgcgaaat gttttagaaa
     4861 aagacctgca atctcggttc cagaaactgg ggatcgactt atctctctcc tctgtttcgg
     4921 ttccctctta tatccctcta aatagatttt taggggagct acacgtcgct cccctgggag
     4981 tgggaggtcc ggagaggctt tcctcctttt ctcttgtccg tgatccttca cctccttctt
     5041 cttccgatca cgactctgac tttgggcgtc aggctaaagc tctggaagac aatctttcct
     5101 ggatgcggac agaaactctg gctcgcaaga agtctctgga agaagtagta gaagtagcgc
     5161 agcaaagaga caggttgcta tatgtggtac ctatgcgctg gcctacttgg ggcagggtct
     5221 cttccaatta tggatggcgt aatgacccct tcacaggaag aagagcctgg cacacaggtg
     5281 tggacattgc tgcccccacg ggaagaaacg tggtagccac agcggaggga aaagtgattt
     5341 tcaccggctg gaatggtaat tatggaaagt gtgtgattgt tcgccatcaa tttggttacg
     5401 agacagtata cgggcacctt tccaaaattt tagtgaatac tggcgatgca gttaaaaaag
     5461 agcaaatcat cgggcaagtg ggaagtacag gaagaagtac cggtcctcac cttcactatg
     5521 aagtgagaag gtacagtgag gtgattaatc cctggcctca tcttccatag aaaagaaaag
     5581 ggggaagaag tgaagcagaa tagcataata ggaatagtga ttattttcct tctgtgtttt
     5641 tcctggcctg tctggggtca agtgggaaaa gtggtgataa ccgacgtctc tgttcaagag
     5701 ttagcaggag acgtttccat ttctattcga ggagaaattc aagcagaagg cgaaggaagc
     5761 atatttttgc aagcttccac cacttacgag aataaagaat attttactgc accccgggag
     5821 ataaaatacg ccctgtctcg agaggccttt tctctttctt tttcccggga agattttgag
     5881 ctggtggact taaaggaaaa taaggtagag aagctacccc agctggcaaa aggagagaga
     5941 ttggtagtct acgtttatga aaagaaaatt acctccccgg aaggacaaac cgaggaagta
     6001 aaacaagaca ttgaaagaac cggttacgct ctgcggggga tactggctaa agccaccagg
     6061 gttttagaat tttaaaagca ctcgcttttt ctttttgtgt gttctcccct gaggggggtt
     6121 ataaggggga ttcttcttgt agtcggggag acgtgcttct tctttcttat atttaaccgc
     6181 aaaatttatg gaatatcctt ttttggccat ataagcctcg catagcagcc tgacggtgtg
     6241 gaaaaaagca ggaaagacga aaaaccagga taacaacgct ggaaaaaact ccagcatgcg
     6301 cgctaacccc tcgagtggtc ataatgagcc tcgtagcatc gcatgtgtgg cagttccggg
     6361 ggctataagg gggttctttt ctctctgcct gaaacctaat caggcatcca aaggtttttg
     6421 cc
//
LOCUS       DAOPSS010000089         6353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_10069_length_6353_cov_8.435059, whole genome shotgun sequence.
ACCESSION   DAOPSS010000089 DAOPSS010000000
VERSION     DAOPSS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10069_length_6353_cov_8.435059"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1040
                     /locus_tag="PKN31_06025"
     CDS             303..1040
                     /locus_tag="PKN31_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOA99516.1"
                     /translation="MRKILTWGVLIFVPFLFFLPCSFAQEAQNDQPMVQDVQETPQEA
                     RTDKEVILEKAEKIRYDSAGETFVATGGVVAVQGANRIQCQELTFNLKNNKGSFKGEV
                     IVTRDETEIRSSFMEGDFDAEIYLFKEGVELKKERKEEGGETSFIFWKAPLLYYNGNT
                     EEAWGEEGAEIEWKETKIKADQAHYYPEKEETGEEERIVLEGNVLITEKDREMEVAKA
                     VYYLDTEVLEAEGIIHARFLIQEKSEE"
     gene            1030..3030
                     /locus_tag="PKN31_06030"
     CDS             1030..3030
                     /locus_tag="PKN31_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99517.1"
                     /translation="MRNEVFRLGVFSLFIIFLFAFSVGAQEALELTGERLQYRLGEEE
                     VVVYEGKVSYKDISLTADYIHIFLEKKELEAEGKVVVERGEEGAQAEKAYYNWEMDWL
                     SLEKVRSDLTGKSIEGKFYFRGEEVKEEKIDGERKTEVEGAHLTSCDLEEPHYYIEAK
                     RLVIYPDEKVEIYDLSYWDFGHRLLSLPFYVFFLNRKEQLPFLPVVGHSADLGFYFSY
                     FYNYFVNDYSFGTITLDWWQHGGWGLGVTHYLEREGEEGRLTIYYRDPASSSSTLRGS
                     VKYSKEVDEFTDLSLNLDYSGTLGEADDRYSGFISLLRAKDKSTSKLNLSYSANESSN
                     TENLYTTWNYQYKLRDDLTGELNLNYKEYEKRGEFVDQDLAYGVTFKKTSDLYTYYLR
                     YSGHTDLEGDAYTGDSNKVVFKIPELEVARGKEKIGESDFTYKAGLLLGHYFEEETGV
                     TDERLRLTLNLEGKSEVGGGDLTSFFNFQQDFYGNGFARYIWLGGIKWVDEISPDLSL
                     TLSYQRGDYDGATPFKFDYVRPRTNYLSLGLDYDRSPWGVSLDTGYDFREEEFVDALF
                     SIEYNLEDNKKVELSGSYDITDNEWGSVVFGIAWPMNKEWGIDFSGRWDPQEGEIDGV
                     RVGLTNDLHCREISLFYDQSRDTFWLEYGIKALPGEKIKLGG"
     gene            3035..3556
                     /locus_tag="PKN31_06035"
     CDS             3035..3556
                     /locus_tag="PKN31_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99518.1"
                     /translation="MKNKFFLLVVVALLAVGCARGEQPDWGEGGKNLYITTTWQEISS
                     SYVYYVAISTDSGLIPDSDPDRWNEFYVVKWEDHNFFLRKPGGSFSYFSSPSVSGQEM
                     RVTLSLTDDLGDPNIIRVMVVSTDKGGNVEDFLDEVATFRLKQQSSEPPIYDLAGDAD
                     AAADLLSLKIEVR"
     gene            3557..4729
                     /locus_tag="PKN31_06040"
     CDS             3557..4729
                     /locus_tag="PKN31_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOA99519.1"
                     /translation="MKNKRGKLFWIIFIPFLFTGCFNSGVVPPVVDKGTSWTVMVFMN
                     GDNDLYKYAWRDFNSLEKVGSTDQVKVIVQFDSLGGEAARYLVKKDAFPNLVTSPVLE
                     YLGEVNMGDASGLTDFVRFCAENYPADKYALIIWDHGTGFKNKDISFDFSPEDAITIP
                     ELGMALSLSTMYLGKKIDFLGMDACLMAMMEVAYEVKNHARVMVTSQELVPGEGWNYE
                     AILRGLVSRPMMGERDLARLVVNSYIDYYPVGSFTQSAIDLGGISSLGKALDELSRDI
                     LNDSLTSPLVYLSLGDSAVFFGDADYVDLGSLVSLFSQDSRVKSEEVRESARLVQERL
                     ERCVIYERASGEMAGNARGLSIYFPYFPYNSKYDDLAFSRDTHWDEMIKYLSQWRR"
     gene            4758..5789
                     /locus_tag="PKN31_06045"
     CDS             4758..5789
                     /locus_tag="PKN31_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HOA99520.1"
                     /translation="MDRYEIQIWRTLKKGPCSFWKLLDEQDEHIKGFVERLKTMMEKG
                     WVVYEGGQFSLSLQGEKLAASISPAQEVRCPHCRGGYNFHAFPEAWELYSKLVESRPL
                     PDPRFDQGFMTQEDIFARVAFMYERGDIEGQEILLLGDDDLFSLALLATGFPRSVTVL
                     EVDSRLVDFIEKRGKENNFNLEVYHYNAVDPYPLEDHAFSVFVTDPVESEKGLKVTLS
                     RGAQALRSEGALYFGLTTIESSWQKWYNIEKMLLEMNFVVTDILRRFSSYPDTDNQFD
                     ESYYDQTRMRKLLEVELPLPADADWFRSHFLRCEAVGKPHPLIRDGVDFDASFYLDDE
                     TLATPGWEI"
     gene            6064..>6353
                     /locus_tag="PKN31_06050"
     CDS             6064..>6353
                     /locus_tag="PKN31_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA99521.1"
                     /translation="MKTMKKIVLAVVLVLAFALPVLANPFVDVPLNHWAYDAVQSVAA
                     KGIFIGYPDGTFGGNRAATRYEFAMATARMLAYMEQRIDEAGFATQEDVAML"
BASE COUNT         1771 a         1140 c         1687 g         1755 t
ORIGIN      
        1 tactataagg gggcgctgcc ccctcataac ccccgaaaaa acaaaaaaca taaaaaaata
       61 aaagaccact gagggaggct ttttctccct cagtaaaaag atttggattc ccgattaaag
      121 atttcaggaa tgacggatga ggtggatgcc tcgctaaatt tcaggcatga caaaaagaga
      181 agccctcgga gtgagaggga cggtttagag agcttgcggg gagagggtta gggtgaatca
      241 gctataaaga tgtgtgttta atgatataat aaccaaaaat tgtggtcaag cgagggataa
      301 acttgagaaa aatattaaca tggggagtgc tgatttttgt tccttttctc tttttccttc
      361 cctgctcttt tgcgcaggaa gcccagaacg accaaccaat ggttcaagat gttcaggaaa
      421 ccccacaaga agcccggacc gacaaagaag tgattttaga aaaagcagaa aaaatcaggt
      481 atgactctgc cggggaaact tttgtggcta ccgggggagt ggtggctgtc cagggggcaa
      541 atcgcattca atgccaggaa ctcactttta atcttaaaaa taataaaggt agtttcaagg
      601 gagaggtaat agtcacaagg gacgaaacag aaatccgttc ttcttttatg gagggcgatt
      661 ttgatgccga aatttatctc tttaaagagg gagtggagtt gaaaaaggaa agaaaagagg
      721 agggaggaga gacctccttt atattctgga aggctcctct tctttactat aacggaaata
      781 ccgaagaggc gtggggtgaa gagggggcag agattgagtg gaaggaaacc aaaataaaag
      841 ccgaccaggc tcattactat ccagaaaaag aagaaaccgg ggaagaagaa aggatagttt
      901 tagaaggtaa tgtcctcatc actgaaaaag accgggaaat ggaagtagct aaagcagttt
      961 actatctgga tacggaggtt ctggaggccg aagggattat ccatgctcga ttcttaattc
     1021 aagaaaaaag tgaggaatga agtgtttcgc ctgggagtgt tttccctctt tattattttt
     1081 ttatttgctt tttctgtggg ggcacaggag gctttagagc tgaccggaga gcgcctgcag
     1141 tatcgtctgg gagaagagga agtggtggtt tacgagggga aggtatctta taaagatatc
     1201 tctctcactg ctgattatat ccatattttc ctggaaaaaa aagaactgga agcagaaggg
     1261 aaagtagtag tggaaagagg agaggaggga gcgcaagcag agaaagctta ttacaactgg
     1321 gagatggact ggctgagttt agaaaaagtg cgctctgacc tcaccggtaa atctattgag
     1381 gggaagttct attttcgagg agaagaggtt aaagaggaga aaatagacgg ggagagaaaa
     1441 acagaggtgg aaggcgctca tttaacgagc tgtgatctcg aggagcccca ttactatata
     1501 gaagctaaaa gactggtgat ttatcctgat gaaaaagtag aaatttatga cctttcttac
     1561 tgggattttg gccatcgtct cctctcttta cctttttatg tttttttctt aaatcgcaaa
     1621 gagcagttgc ctttcctgcc tgtcgtggga cactcagccg acctgggttt ttacttcagc
     1681 tacttttata actattttgt gaatgattac tcctttggca ctatcactct ggactggtgg
     1741 caacacggag gatggggtct cggcgtcact cattatttag agcgagaagg agaagagggg
     1801 aggcttacca tctattatcg agacccggct tcttcttcct ctactctccg ggggagcgtt
     1861 aaatacagca aagaagtcga tgagtttact gatttgagct tgaatctgga ctattctggt
     1921 actttggggg aggccgatga ccgttattct ggttttattt ctcttttgcg agcgaaagat
     1981 aaaagtacca gtaagcttaa cttgagctac agcgctaatg agagcagcaa taccgaaaat
     2041 ctctacacca cctggaacta tcaatataag ctgagagacg acctgactgg agagttgaac
     2101 ctcaactaca aagagtatga aaaaagagga gagttcgttg accaggacct tgcctacggg
     2161 gttactttta agaaaacttc tgacctttac acttattacc tgcgatatag tggccacaca
     2221 gatttggaag gagacgccta cactggtgac tccaataaag tggtattcaa aatccccgag
     2281 ctcgaggttg ctcgaggcaa agagaaaatc ggagagtcag attttaccta taaagctggg
     2341 ctgctcctgg gccactattt tgaagaagag accggagtaa cagatgagcg cctgcgcctc
     2401 accttgaacc ttgagggcaa gagtgaggta gggggaggag acctcacttc tttcttcaac
     2461 tttcaacagg atttttatgg taatggtttt gcccgctata tctggctggg agggataaag
     2521 tgggtggatg aaatatctcc tgatctgtct ttgactcttt cttatcagag gggtgactac
     2581 gatggagcca ctcctttcaa atttgattat gtccggcccc gaactaatta tttgagttta
     2641 ggcctggatt acgaccgaag cccctgggga gtgagtttgg ataccggata tgactttcgg
     2701 gaagaagagt ttgtagatgc tttattcagc atagagtata acttagagga taataaaaaa
     2761 gtagaattga gtggtagtta tgatatcact gacaatgagt ggggaagcgt ggtctttggc
     2821 attgcctggc ctatgaataa agagtggggc attgattttt caggaaggtg ggatcctcaa
     2881 gagggagaga tagatggagt gcgagtaggg ttgaccaacg acctccactg ccgggaaata
     2941 agcctttttt atgaccagtc tcgggacact ttctggttgg agtacggaat caaagctctt
     3001 cccggagaaa aaataaaatt aggaggataa atcgatgaaa aataagtttt ttcttcttgt
     3061 cgtggtggcg cttcttgcgg tgggttgcgc tcgaggagaa cagcccgact ggggagaagg
     3121 agggaaaaat ctctatatca ctaccacctg gcaggaaatt agctcttctt atgtatatta
     3181 tgtagccata agcactgact ccggactcat tcctgattct gatcctgata ggtggaatga
     3241 attttatgtg gtgaagtggg aagaccacaa cttcttttta aggaaacccg gtggaagctt
     3301 tagttacttt tcttctcctt ctgtgtcggg tcaggagatg agagtgactc tgtctttgac
     3361 tgatgatttg ggcgacccca atattatccg ggtaatggtg gtgtcaacgg ataaaggagg
     3421 aaatgtagaa gattttctgg atgaagtggc gacatttcgg cttaagcaac aaagcagtga
     3481 gcctccaatt tacgacttag caggagatgc agacgcagca gccgatcttc tctccttgaa
     3541 aatagaggta agataaatga aaaataaaag ggggaagctt ttctggatca tttttatccc
     3601 ctttttgttt accgggtgct ttaatagcgg ggttgttccc ccggtggtgg ataaaggcac
     3661 cagctggaca gtgatggttt ttatgaatgg agataacgac ctgtataaat atgcctggcg
     3721 ggattttaac tctttagaaa aagtgggctc caccgaccag gtgaaggtca ttgtgcaatt
     3781 tgattctctc gggggagaag cggcccgata tcttgttaag aaagacgcct ttcccaattt
     3841 ggttacctct ccagttttag agtatttagg ggaggtaaat atgggagacg cttcagggct
     3901 taccgatttt gttcgttttt gtgcggaaaa ttatccggcc gacaaatatg ctctcatcat
     3961 ctgggaccac ggtacgggat ttaaaaacaa agatatttct tttgatttct cacccgaaga
     4021 tgccattacc ataccagaat taggaatggc tttgtctttg tccactatgt acttagggaa
     4081 gaagatcgac tttctgggaa tggatgcctg tcttatggct atgatggagg ttgcttatga
     4141 ggttaaaaat catgcccggg tgatggttac ctctcaagag cttgtgcccg gggaagggtg
     4201 gaattacgag gccattttga gaggcttggt ttctcgcccg atgatggggg agagagacct
     4261 ggctcgtctt gtggtgaata gctatataga ctattatccg gtggggagct tcacccaatc
     4321 ggctatagac ctgggaggga tttcctctct cggcaaagca ttagatgaac tgtcccggga
     4381 catattaaat gactcactca cttctccttt ggtttatctc tctttggggg attcggcagt
     4441 cttttttgga gatgcagatt atgtagacct gggtagttta gtaagtttat tctctcaaga
     4501 ttctcgagtt aaaagcgaag aggtgagaga aagcgctcgt ctggtgcagg aaagattgga
     4561 gagatgcgtt atttatgagc gtgcttctgg agaaatggca ggaaatgccc ggggcttgag
     4621 catttatttt ccttactttc cttataattc caaatatgat gatttggcat tttcccgtga
     4681 cactcactgg gatgagatga taaaatattt atcacaatgg cggaggtgag ggggagactt
     4741 tagaaaggag caaagttatg gatcgttatg agatacagat atggcgaact ctcaaaaagg
     4801 gtccttgtag tttctggaaa ttattagatg agcaagatga gcatataaag ggttttgtgg
     4861 aacggctcaa aactatgatg gagaagggct gggtggttta tgagggagga caattttccc
     4921 tctctctgca gggagaaaag cttgccgctt ctatctcccc ggcgcaggag gtgagatgcc
     4981 ctcactgtcg aggggggtac aattttcacg cctttcccga agcctgggag ctttattcta
     5041 aactggtaga aagcaggcct cttcccgacc cccgtttcga ccaggggttt atgactcagg
     5101 aagatatttt tgcccgggtg gcgtttatgt atgagcgagg agacatagaa gggcaggaga
     5161 ttcttcttct gggggatgac gaccttttca gtctggcttt gttggctacc ggatttcccc
     5221 ggtcggtaac tgttttagaa gtggatagcc gcctggtaga ttttattgaa aaaagaggca
     5281 aagaaaataa ttttaatctg gaagtttatc actataatgc cgttgacccc tatcctctag
     5341 aagaccatgc cttttctgtt tttgtcactg acccggtaga aagcgaaaaa ggtttaaaag
     5401 ttactctttc ccggggggct caggctcttc gctcagaagg agctttatat tttggtttga
     5461 ctactattga atcttcctgg cagaaatggt ataacataga aaagatgctt ctggagatga
     5521 actttgtggt aacggatatc ttgcgtcgtt ttagttctta tcctgatacc gataatcagt
     5581 tcgatgaaag ttattatgac cagaccagaa tgagaaagct tttagaggtg gaattgccgc
     5641 tacctgccga tgcggactgg tttcgttctc attttttgcg ttgtgaggcg gtgggaaagc
     5701 ctcacccctt aattcgagac ggggtagatt ttgatgccag tttttactta gatgatgaga
     5761 ctctggctac cccgggctgg gaaatttaaa aaatttttta aaaatttctt tttttgttgt
     5821 agtcaaaagc aataatttgt cgtatagtat atataggata aaaaaaggct atcctgtctc
     5881 ttactcctgg gtaaggagaa aggaggtgaa aaaggattct tggtgacccg gtgagaagag
     5941 atgtaaggtg gcagctggaa gtctgagaaa aggagagaga agagaggaaa cagggatact
     6001 ctcggtgatt ctccttggaa gacggaaggc tgaataaaac aaactaccaa ggaggtttca
     6061 aacatgaaaa ccatgaagaa gatcgtatta gcagtagttc tagtgctggc ttttgctctt
     6121 cctgttctgg ccaatccgtt tgtggatgtg ccactgaacc actgggctta tgatgcagtg
     6181 cagagcgtag cggccaaggg catttttatt ggttaccctg atggcacttt tggtggtaac
     6241 cgagctgcaa ctcgttatga gtttgccatg gctactgctc gaatgctggc ttatatggag
     6301 cagagaatag acgaagccgg atttgctact caagaagacg tagctatgct gga
//
LOCUS       DAOPSS010000090         6317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_10126_length_6317_cov_8.800064, whole genome shotgun sequence.
ACCESSION   DAOPSS010000090 DAOPSS010000000
VERSION     DAOPSS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10126_length_6317_cov_8.800064"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PKN31_06055"
     CDS             complement(<1..1094)
                     /locus_tag="PKN31_06055"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HOA99522.1"
                     /translation="METYLVLTIDIGTSSCKVCVFDARGEILKTSKREYPAYSPAPGF
                     AEQNPEEIYQAIKKAVKEALEDFSPREVEFVSFDTMLHSFLLVDREEVPLYPLLNWMD
                     TRCADEVERLKPLYRERKLYFKNAAPLHTIYHIPRTWWFKKNQPHLWEKTSRVLSIKD
                     WILEKITGELFCDYSTASATGLLNLSSFSWDEEILDFAGISLDKLPPLEAPEHTAPLK
                     EGDFTKDTGLLPGTPVIWGGGDGPFANLGDGAFREGEMVVTVGSSGAVRMCSPHPVFD
                     PEERSWCYYLADGIWVGGGAINNGGIVYSWIKDLVGEEDWEWDIHRQRPLFLPFLTGE
                     RSPNWNAYARGILFGLSYFHERSALLQAAF"
     gene            complement(1097..1921)
                     /locus_tag="PKN31_06060"
     CDS             complement(1097..1921)
                     /locus_tag="PKN31_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOA99523.1"
                     /translation="MSYVEELFSLQDKVVFCTGGGGILMSAISEGLGKAGAKVILTDI
                     VPLEERVEALKKEGIEAKGYYMDAMDKKSVEEVAKKVKEEFGGIDALLNAAGGNREDA
                     TTSNEVSFFDLPLEPLQKVVMLNLFGGAIIPSQVFARMMIEDGREGNIINFSSMNAFR
                     PLTRVVGYSAAKAAVSNFTQWLAVYLAKEYSPKVRVNALAPGFFITNQNRYLLTKEDG
                     SLTPRGEAVINHTPLGRFGVPEDLIGTVIWLLSPASSFVTGVVVPIDGGFNAFSGV"
     gene            complement(1963..3459)
                     /locus_tag="PKN31_06065"
     CDS             complement(1963..3459)
                     /locus_tag="PKN31_06065"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOA99524.1"
                     /translation="MSDFSKTVEIIKRLEERMSLSLRVYSSSWYQEKLGMRIYPVKGE
                     EERYLGVWSKDKKLYFADPLFLEPEEEKGKFFFLYPFHFKNYLSLSDYFPDLKPQPAG
                     VKTSLGCGDRLGLVSRAHLEAVKNYPAFPVIAQQSPRELQKMGRTFQDVLLGAVWGVL
                     ESENPVPFGADADHLKDEEYLRAGIESGFTMYTLDGSGVADYYILSKSEKELQKIFDS
                     MSQEEKQIFNRYADRTFTVGSGLELDFQRKKFFPLFKVYFPVINFVEKMNFLLQEELS
                     SYDLEISLDEGEGVTSPEVHFFVAEELHHRGIDFQSLAPRFPGSFEKAIDYEGNIQEF
                     SSSLRNHVTVQQEIGGYRLSLHSGSDKFSIYPVFSQETNQLFHVKTSGTSWLKGLEVV
                     AHKNPQLFRKIYELSEEELEENRKAYQISFTREDIHRDMDTIPDKELPSLFSHPLIRQ
                     FLHISYGSVWQVFKSELSHLFFQYEEEHYRLVRENMERHLHFLFSLNK"
     gene            complement(3524..4921)
                     /gene="aglA"
                     /locus_tag="PKN31_06070"
     CDS             complement(3524..4921)
                     /gene="aglA"
                     /locus_tag="PKN31_06070"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032450 - maltose alpha-glucosidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase AglA"
                     /protein_id="HOA99525.1"
                     /translation="MVKISFMGAGSIRYTLKLVGDLAKTKELSGSLVSLMDVDEERLE
                     AVYDLAKRYIDEVGGNLKLEKTTDREKSLQGADFVINTALYRAPGHEDGYVSYEIMRD
                     LAERYGYYRGIDSQEFNMVSDYYTFTNYNHLKLSLDIAKSIEEICPDAYFIQTANPVF
                     EITELVLRHTNVKVVGFCHGFSGLFEVFHTLGLNPEEVDWQVAGVNHGIWLNRFLYRG
                     ENAYPLLDKWIEEESQHWQPENPWDVHLSPAVIDMYRFYGLLPIGDTCRNGTWKYNYN
                     LDAKKKWYGKFGGIDNEVERPRFYEELRQRKRMMIELSKDPGIKLTKSWPEEFSKDKM
                     SGEQQIPFINAIVNNKATRLVLNILNRGAIKGISDDVVVEIPLEVNGSGIHSEKIEPN
                     LPDRILKYYLTPRITRMEMAVDAFTTGDRKILEELLVRDPRTENYEDIPKLWNEIFSL
                     PFNQEMKSHYSGAKI"
     gene            complement(5037..5246)
                     /locus_tag="PKN31_06075"
     CDS             complement(5037..5246)
                     /locus_tag="PKN31_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99526.1"
                     /translation="MGRKNDSFSKTREIVKKIGERIIFPLRIYSSSDYQELGMTIYPV
                     KGEKRDIKESPFSGWKRSSQLPAVF"
     gene            complement(5268..6062)
                     /gene="iolB"
                     /locus_tag="PKN31_06080"
     CDS             complement(5268..6062)
                     /gene="iolB"
                     /locus_tag="PKN31_06080"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOA99527.1"
                     /translation="MLFFPYQKYPDNWLYQVVDSSNSPLQMESFSLLKLSPHTSYQGE
                     LKGEEAVFVLLQGKVKMEVEGKTLGEIAQRKSIFEDKASAFYLPPGVYYQIEAEEDSE
                     LILVRAKAEKGGSPAFFPPEIVKVKTTGKGTFEREVHTIVGEDFPAEKLIVGETFNKP
                     GLWSSYPPHRHEKNRPPEEYFLEEIYHYRVEPEKGFGIQVLYTEDKELEKAYLVENGD
                     TFVIPRGYHPVVAAPGYRLYYFWSLAGEVRILNPYDDPHHSWTKNL"
BASE COUNT         1836 a         1525 c         1043 g         1913 t
ORIGIN      
        1 tcaaaagcag cctgaagaag agctgacctt tcgtgaaaat aggataaccc gaataaaatc
       61 ccccgagcat aggcattcca gttggggctc cgctctccgg ttaaaaaagg aagaaagaga
      121 gggcgttgtc gatgaatatc ccactcccaa tcttcttccc ctaccagatc ttttatccag
      181 gaatagacaa ttcctccgtt atttatagct cctcctccta cccatatccc atcagccaga
      241 taataacacc agctccgctc ctcggggtca aataccggat gaggagaaca catcctgact
      301 gccccagatg agcctaccgt taccaccatc tccccttctc ggaaagctcc atcacccaaa
      361 ttagcaaagg ggccatctcc cccgccccag ataaccggag tcccgggaag gagccctgta
      421 tctttagtaa aatctccctc ttttagaggg gcggtgtgtt cgggagcctc aagaggggga
      481 agtttatcaa gagaaattcc cgcgaaatca agaatctctt catcccagga aaaggacgaa
      541 aggttcaaga gaccagtagc cgaagcggta gaataatcac agaaaagttc cccggttatt
      601 ttctctaaaa tccagtcttt tatgctgaga actcgagaag ttttctccca cagatgaggt
      661 tgattttttt tgaaccacca ggtccggggg atgtgatata tggtgtgaag aggagcagca
      721 ttcttaaaat agagcttccg ctctcgataa agaggtttta atctttccac ctcatcagca
      781 catctcgtat ccatccagtt aagaagagga tacagaggaa cttcttctct gtctaccagg
      841 agaaaactat ggagcatggt gtcaaaagaa acaaactcta cttctcgggg agaaaaatct
      901 tccagcgcct cttttactgc tttttttatt gcttggtaga tttcctccgg gttttgttcg
      961 gcaaaacccg gagcaggaga ataagcagga tattctcttt tgctcgtttt taagatttct
     1021 cctcgagcat cgaatacaca tactttacag gaagaagttc caatatctat agtgagcacc
     1081 aaataagttt ccataactat acccctgaaa aagcgttaaa ccctccatca ataggcacta
     1141 ccactccagt aacaaaagaa gaagcaggag aaagaagcca tattactgtc ccaatcaaat
     1201 cttctggtac tccaaaccgc cccaggggag tgtgattaat aaccgcctcc ccacgagggg
     1261 ttagactgcc atcttcttta gtgagaagat aacgattctg gttagtgata aaaaaccccg
     1321 gagccagagc attcactcgc actttcgggc tgtattcttt agctaaataa actgctaacc
     1381 attgagtaaa gttactcact gccgctttag ctgcagagta ccctaccacg cgagtgagag
     1441 gacgaaaagc attcatggag gaaaaattaa ttatattccc ctcccgacca tcttctatca
     1501 tcatgcgggc aaaaacttga gaaggaatta ttgctccacc aaataggtta agcataacaa
     1561 ctttttgcag aggctcgaga ggtaaatcaa aaaaagaaac ctcattggat gtcgtagcat
     1621 cctcccggtt ccctcccgca gcatttaata gagcatctat tcctccaaat tcttctttca
     1681 ccttttttgc cacttcctct acacttttct tatccatagc atccatatag tatcctttgg
     1741 cttctattcc ttcttttttt aaagcctcta ccctttcttc taaaggaact atatcagtta
     1801 ggataacttt cgctcctgcc tttcccaaac cttcactaat agctgacatc aaaattccac
     1861 ctcctccagt acaaaatact accttatcct ggagagagaa aagctcctct acgtaagaca
     1921 ttttgttcgc tcctttctga ttatattcct gtctggttta aattatttgt ttaaagaaaa
     1981 tagaaaatga agatgtcgct ccatattctc tcttactaaa cgataatgct cctcttcata
     2041 ctggaaaaag agatgactga gttccgactt gaaaacttgc cagactgaac cataagaaat
     2101 atgcaagaat tgacgaatta aagggtgaga aaaaagggaa ggaagctctt tatccggaat
     2161 ggtatccata tctctatgga tatcttccct ggtaaaagaa atctgataag ctttgcgatt
     2221 ttcttctaac tcctcttcgc ttaactcata aattttccgg aaaagttgag ggtttttatg
     2281 agccactact tccaggccct taagccagct ggtacctgag gttttaacgt ggaaaagctg
     2341 attagtttct tgagaaaaaa caggatagat gctgaactta tcacttccag aatgcagact
     2401 cagtcgataa cctccaatct cctgttgcac agtcacatga ttgcgcaggg aagaggaaaa
     2461 ttcttgaata ttcccctcgt agtcaatggc cttttcaaag cttcccggaa aacgaggtgc
     2521 taaactttga aaatctatgc ctctgtgatg cagctcctcg gctacaaaaa aatgaacttc
     2581 gggagaagtt actccttctc cttcatccag cgatatttcc aaatcatacg aagaaagctc
     2641 ctcttgcaaa agaaaattca ttttttctac gaaatttatc actggaaagt aaaccttaaa
     2701 aagaggaaaa aattttttcc tctggaaatc taactctaaa ccgcttccaa cagtaaatgt
     2761 ccggtcggca tatcgattga agatttgctt ctcctcctgg gacatactat caaatatttt
     2821 ttgcaactct ttttctgatt tagaaagtat atagtaatca gccactccac ttccatccag
     2881 ggtgtacata gtaaaaccgc tttctatccc tgctcgaaga tactcctcat ctttcagatg
     2941 gtcagcatct gcaccaaaag ggacaggatt ttcactttct aatactcccc agacagcacc
     3001 gagtagcaca tcctgaaaag ttcttcccat tttttgaagc tcccgggggg attgttgagc
     3061 aatcaccgga aaagctggat aatttttaac cgcttctaag tgagcccgag acactaaacc
     3121 caacctatcc ccacaaccta aagaagtttt tactcctgcc ggctgaggtt tcaagtcggg
     3181 aaaataatca cttaaggaca aataattctt aaagtgaaaa ggataaagga agaaaaattt
     3241 ccctttttct tcctctggtt ctaaaaaaag aggatcggca aagtaaagtt ttttgtcctt
     3301 cgaccacact cctaaatacc tctcttcttc tcccttcacc ggataaattc tcattcccaa
     3361 cttttcctga taccaggaag aagaatagac acgcaaagag agactcattc tctcttctag
     3421 cctttttatt atctctaccg ttttagaaaa atcgctcatt ttttcctcct gcttattttg
     3481 aaattatatc taatttaaag gtttcaaaac ctttccagcc tgtttagatt tttgcacctg
     3541 aataatgact cttcatctcc tggttaaaag gtaaactgaa tatttcattc cataacttcg
     3601 gaatatcctc atagttttcc gttcttggat ctcttaccag aagctcttct aatatttttc
     3661 tatctcccgt agtaaaagca tctacggcca tttccatcct tgttattcta ggtgtcaaat
     3721 agtattttaa tattctatct ggcaaattgg gttctatttt ttcagaatgt attccacttc
     3781 cgttaacctc aagaggtatc tcaacaacta catcatcaga tattcccttt attgctcccc
     3841 gatttagaat attcaagact aatctagtgg ctttgttatt cactattgca tttatgaagg
     3901 gtatttgctg ttccccgctc atcttgtctt tagaaaactc ttctggccag cttttagtta
     3961 attttatccc ggggtccttt gataattcta tcatcattct ttttctctgt cgaagctctt
     4021 cataaaacct gggtctctct acctcattat ctatacctcc aaacttccca taccacttct
     4081 ttttagcatc gaggttataa ttatatttcc aggttccatt gcgacaggta tctcctatcg
     4141 gcaacagacc gtaaaacctg tacatatcta tcaccgcagg acttaaatga acgtcccagg
     4201 ggttttctgg ctgccagtgc tgagattctt cctctatcca tttatcaagc aaaggataag
     4261 cattttctcc ccggtaaaga aagcggttta accatattcc atggttcacc ccggcgactt
     4321 gccagtccac ttcttctgga ttcagcccga gagtatgaaa aacttcaaac aatcctgaga
     4381 acccatgaca aaaaccaacc accttaacat tggtatgcct taaaaccaac tcggttattt
     4441 caaaaaccgg gttagctgtt tgtatgaagt aagcatcggg acagatttct tctatagatt
     4501 ttgctatatc cagggaaagt tttaaatgat tataattggt gaatgtataa taatctgaca
     4561 ccatgttaaa ctcttgacta tctattcctc tgtaatatcc gtatctctcc gctaagtctc
     4621 tcataatctc atagctaaca taaccatctt catggcctgg cgctcggtac aatgctgtat
     4681 tgatcacaaa atctgcccct tgtagcgact tttctctatc cgttgttttt tctaatttta
     4741 agtttcctcc cacctcgtct atatatcttt ttgccaggtc atacactgct tctaatcttt
     4801 cttcatccac atccattaat gaaaccaaac ttcccgaaag ttctttagtt ttcgctaaat
     4861 ctccaaccag cttaagcgta tatcttatac ttcctgcccc cataaaagat attttcacca
     4921 atcttgtgcc tcctctacca gtaattcgta aactatttca aataaagtaa aaagccgttc
     4981 tcgggccaat ctctaagcca gaaatattgc gagcagctta taattctttt tctagttcag
     5041 aaaacagctg gcagctggct actccgcttc catccagaga aaggagactc cttaatatct
     5101 cttttttctc cctttaccgg ataaattgtc attcccagct cctgataatc actagaagaa
     5161 taaattcgca aaggaaagat tatcctttcc cctattttct taactatttc ccttgtttta
     5221 gaaaaactgt cattttttct tcccataatt tcgaaaacat aatttgctta aaggttcttt
     5281 gtccaagagt gatgcggatc atcataaggg ttcaatatcc tcacctctcc agccaaagac
     5341 caaaagtaat aaagcctgta ccctggagcc gctaccaccg gatgatagcc ccgggggata
     5401 acaaaagtgt caccgttttc taccaaatag gctttctcta actctttatc ttcagtatag
     5461 agaacctgaa tcccaaaccc tttctctggc tctacccgat agtgatagat ttcttccaaa
     5521 aaatactctt caggagggcg atttttttca tggcgatggg gaggataact cgaccacaat
     5581 cccggtttat taaaagtctc tcccactatt aatttctccg ctgggaaatc ttctcccact
     5641 atagtgtgca cctctcgctc aaaggtgcct ttacctgtag tcttcacttt cactatttcg
     5701 ggagggaaaa aagcgggaga gcctcctttt tctgctttag ctctaaccag gataagctcg
     5761 ctatcttcct ctgcctcaat ctggtagtaa actccagggg gaaggtaaaa agcagaagcc
     5821 ttatcctcaa agatagactt tctctgcgca atttctccta aagttttccc ttctacttcc
     5881 attttcacct tgccctgtaa aagaacaaat accgcctctt ctcctttgag ctctccttga
     5941 taagaagtat gaggagaaag cttaagcaga gagaaactct ccatctgaag aggactatta
     6001 gatgaatcaa ccacttgata aagccagtta tccggatatt tttgataagg gaaaaagagc
     6061 atttttgata cctccttaca atttcaaata ataaagcgat ttttccacca aacctgtcaa
     6121 ctaatctctc ctttaaacgt accccatcta aatcatttct attttcttag caaattgaca
     6181 aatacaatag attaattcaa ctacaaacca ttatttcagc aattctttca cctttccaac
     6241 aatatttgat actgataaac caaaattttc cctaagttct tcaaggtctc ccgagcaacc
     6301 gaaatgatcg tttattc
//
LOCUS       DAOPSS010000091         6128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_10521_length_6128_cov_7.205664, whole genome shotgun sequence.
ACCESSION   DAOPSS010000091 DAOPSS010000000
VERSION     DAOPSS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10521_length_6128_cov_7.205664"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKN31_06085"
     CDS             <1..308
                     /locus_tag="PKN31_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99528.1"
                     /translation="DIIPLFIEGGITGIYPIEVSCGMDLVKVRKSFPHLQIMGGIPKG
                     ELKYGRDRIDRALKPVEEIIKEGGYIPFVDHLVPPDVSFENFVYYRNKLNQIIDSIG"
     gene            1188..2195
                     /gene="rhaS"
                     /locus_tag="PKN31_06090"
     CDS             1188..2195
                     /gene="rhaS"
                     /locus_tag="PKN31_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA99529.1"
                     /translation="MKKLITILFLVASMVVFFSVSAFAEGYTVGMVVKNVGNPFMDAA
                     GRGGEEACGELGNSFIFQGPATPTVEGQIEIIENLIAQKVDAICITANDFDALVPVLQ
                     KAKGNNIKVVSFDSAVAPGGRHVHVNQADAELIGKLQVQAIAEMIGYEGEIAILSAGA
                     TMTNQNTWIEYMKEELKDPKYEKIELVTIVYGDDLRDKSYNEAMGLFKSYPNLKGIIS
                     PTTVGLSATAKAITDAGLVGKVKLTGLGLPSEMAEWVHNGACEAFFLWNPIDLGYLAS
                     YVAGLLCDGTITGEVGEEFSAGRMGDYTIVEAEDGGTEVLLGPPFRFDASNIDEWKDI
                     Y"
     gene            2249..3799
                     /locus_tag="PKN31_06095"
     CDS             2249..3799
                     /locus_tag="PKN31_06095"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA99530.1"
                     /translation="MGFLEVDSVVDREDRYILELKGISKFFPGLKALDRVDFNLRAGE
                     IHAIVGENGAGKSTLIKIITGVYQPDEGDIFYEGNTVIFHNPTVANRYQIAAVYQQPA
                     SFPHLTVTENIFLGHPIIHPTTKKLMWKEMKEEAKRLLRSLGSDINPSVTMSSLSVAQ
                     QQIVEIAKALSVNARILIMDEPTASLTRRETEDLYNIARKLRNDGVSIILISHLFEDV
                     YEIADRVTVLRDGKSIGTWNVNEIDEKDLIKAMVGREISQLFPKNKVSFGKELLRVEG
                     LSRLGYFSDISFSLRAGEIVGFSGLVGSGRTEVAQSIFGIAPVDAGKILVEGKEVSIK
                     NPMEAISYGIGYLPEDRLEQGLFLLLSINDNITISIIDQLTNRGWLNPPREHEISNKM
                     LELLQIKAPGIFHPVSSLSGGNQQKVVVAKLLAARPKILIMDEPTKGVDVGAKAAIHQ
                     IMSDLVAEGLGIIMISSEMPEILGMSDNIIVMHEGRITGVFSRSEATQEKLMEAAVKV
                     DRLTQVVS"
     gene            3825..4838
                     /locus_tag="PKN31_06100"
     CDS             3825..4838
                     /locus_tag="PKN31_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99531.1"
                     /translation="MMDEPAKTKREVTFSRLLGFRELGIIIFIVLISVLIGIRNPRFL
                     TWSNFHDILIDTAALSVLSVGMMLVLVTGGIDLSMESVLALTGMVAGMMVKQNPQLSP
                     LFMLILGLLLGGLLGSITGLIVAKGRVVPIIATLSMMYIYRGITFIISGGKWINAYEM
                     SDSFKGIATERFLGLSKLIYIVLVVYVVFYYFVNYTHTGRKIYAVGSNVEAARVIGIN
                     TEFITWLVYAISGALSGLAGVMWVARYASAQNDTASGFVMTIVAACVLGGVSITGGVG
                     TVSGVLLGSITVGIINNALPMIRVSPFWKMALQGLIILVAAIVNIAIARNMEKSQLKR
                     RTI"
     gene            4839..5837
                     /locus_tag="PKN31_06105"
     CDS             4839..5837
                     /locus_tag="PKN31_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99532.1"
                     /translation="MKYRNSLSNRKLNIVFKWEWLLVILIIGISIVNGRLSPHFWSYT
                     GIMDSLAVFLEKGFMVLSMTLVLIIGDIDISVASIVALSSVLMAMSYKLGLPMAVAIL
                     VALATGAGCGYLNGLLISLVPGLSAIIVTLAGSSIYRGIAYILLGDQAIGGFPSWYSY
                     LAWGYVGKTNIPFVMVVFVGCAIIYGLLLHRTAWGRKVYAMGINPVAARFSGVPIAQM
                     KQIIFTLNGLMAGIAAIFLTSKLGSSRPNIATGYELEVIAIAVLGGVSPSGGKGGIVG
                     VCLALILMRLLRYGMGLVNVPGQVMMVIIGIVLVGVVMLPNIFSSRGGKRRTKEIQ"
     gene            5843..>6128
                     /locus_tag="PKN31_06110"
     CDS             5843..>6128
                     /locus_tag="PKN31_06110"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOA99533.1"
                     /translation="MSEVKYSSGNMKYEKLKENLLALGRVIVAFSGGVDSTFLLWVSR
                     ETLGENVLGVLVDLPFLPRREREEAEKIARDLRLPLKVIEISGEDEPLIWR"
BASE COUNT         1731 a          986 c         1505 g         1906 t
ORIGIN      
        1 ttgatataat tcctcttttt attgaggggg gtatcactgg tatatatcct atagaagtga
       61 gttgtggtat ggatttggta aaagtaagaa aaagctttcc tcatcttcaa ataatgggag
      121 gtattcccaa aggggagttg aaatacggac gagataggat tgatcgagcc ttgaagccag
      181 tggaagagat aattaaagaa ggaggatata ttccttttgt tgaccattta gttccacccg
      241 atgtaagttt tgaaaatttt gtttattaca gaaataaatt gaatcagata attgatagta
      301 tagggtaggg ggagataggg tagggggaga taatcgttca aaagagaggt ttataccaag
      361 acctcaactt catatgattg tgtatataac agcttaaatc attaaaactg gatggcgaga
      421 cctcaaaaaa gagggaacac catacattgt ttgactgaat gtgacacttt gatagtgtca
      481 ctacaaaaca tggaaaaaac tagaaataga agagtagagt atatattttt tcacgggtat
      541 taggggttag aagagctaaa aaagcagtaa aactgtcaaa gccagggaga gaactactgg
      601 caatcaatgg tcttatgaat acaggctcaa accagcgggg attctgattt caccacgagt
      661 gtacattaaa aaataaattc gcttttgtct aaagatttca gttattaaga ctcgttaatt
      721 aacggtgtaa ttctaagcac tgctttgtaa gagtaagaga caaaggcgtg gtagctttac
      781 aattgatact attttctgac ttaacttaat ctctatcttg ttttgtaaaa ggaataacca
      841 gtaatttctg accttttagg ccagcttgca agcaaggtaa aattcattat attatatgta
      901 ggagggcgtt ctgcctcctt tgggatgatt ttactgatgc aagttctgtg cctagaaaaa
      961 caagtactgt atgcttttat tggtattttt ggtcaactaa ttattcgaga aaatgaccga
     1021 gatcaaaaaa gtagggatat aaaacttagt gaaaatcaaa gaagggaggt gattaaaaaa
     1081 gattttgaag attgacggtt ggcaatagtt tgctggttgg tgttatttag gagtgcagtt
     1141 tttcggtctg aatttaacaa tcaaaataaa ggaggttcca gttagaaatg aaaaagctta
     1201 ttacgattct ttttttggtg gcttccatgg ttgttttctt ttcagtaagt gcttttgctg
     1261 aaggatatac agttggtatg gtagtaaaga atgttgggaa tcctttcatg gatgcagctg
     1321 gcagaggggg cgaggaagcc tgtggtgaat taggtaatag ttttattttc cagggaccgg
     1381 ctactccaac agtggagggt cagattgaaa tcattgaaaa cttaatagct caaaaggtcg
     1441 atgccatttg tataactgcc aatgattttg atgcgttagt accagttctc cagaaggcta
     1501 aaggaaacaa cataaaagta gtatcctttg attcggcggt agcgcctgga ggtcggcatg
     1561 tacatgtaaa tcaggcagat gcagaattga taggtaagtt acaggttcaa gccatagcgg
     1621 agatgattgg ctatgaaggt gaaatagcta ttctttcagc tggagccact atgaccaatc
     1681 agaatacctg gattgaatat atgaaagagg aattgaaaga tccaaagtat gaaaagatag
     1741 aattagtaac tattgtgtat ggtgatgacc tgcgagataa gagctacaac gaagccatgg
     1801 gtttgtttaa gtcttacccc aacttaaaag gcatcatatc tcctaccacc gtagggctca
     1861 gtgctactgc taaggccata actgatgccg gcctggtggg gaaagttaag cttaccggtc
     1921 tcgggcttcc cagtgagatg gcagagtggg ttcacaatgg agcttgtgag gcatttttct
     1981 tatggaatcc catcgatctg ggctaccttg cctcatacgt tgccgggctt ctgtgtgatg
     2041 ggactataac cggggaggtg ggagaagagt tctccgccgg tagaatggga gattacacca
     2101 ttgttgaggc agaagacgga ggaacagaag ttttattagg gcctcctttc cgatttgacg
     2161 caagcaacat tgatgagtgg aaagacattt attgaactag atgcaattga atggtgagcg
     2221 aggatgtatt ctcgctcacc atttttaaat gggatttctg gaggttgata gtgtggtaga
     2281 tagagaagat aggtatatac ttgagctgaa aggaatttcc aaatttttcc ccggtctgaa
     2341 agcattagac cgggttgatt ttaatcttcg cgcaggagag attcacgcta ttgtgggtga
     2401 aaatggagcg ggtaaatcta ctctaataaa aattattacc ggtgtttatc aaccagatga
     2461 aggcgatata ttttatgaag ggaacacagt gatttttcac aaccccacag tggctaaccg
     2521 ttatcaaatt gctgctgttt accagcaacc cgcgtctttt cctcatttaa cagttaccga
     2581 gaatatattt cttggacatc ctataattca ccccactacc aaaaaactga tgtggaagga
     2641 aatgaaagaa gaagcaaaaa ggcttctccg ttctttaggc tctgatatta atccttcggt
     2701 gactatgagc tctttaagcg tagctcaaca acagatagtc gaaattgcca aggctctttc
     2761 agttaacgct cgtatcctta ttatggatga accgactgct tctctcacca gacgggaaac
     2821 tgaagatttg tataatatag caaggaaact ccgcaacgat ggagtgtcta ttatattgat
     2881 ttctcatctg tttgaggatg tctatgaaat cgctgatcgg gtaactgtgc tccgagatgg
     2941 aaagagtata gggacctgga atgttaatga aattgatgaa aaagacctga ttaaagctat
     3001 ggtggggcga gaaataagtc agcttttccc caaaaacaag gtttcttttg ggaaagaact
     3061 tttgagagta gaaggacttt ctcgtcttgg ttatttttct gatatttcct tttctctccg
     3121 ggccggggaa attgttggat ttagcgggtt ggttggttcg ggaagaactg aagtagcgca
     3181 atcgattttt ggcatagccc cggttgatgc aggaaagata ttagtagaag ggaaagaagt
     3241 atcgattaaa aacccgatgg aggctataag ctatggcatt ggatatcttc cagaagaccg
     3301 gttagaacag ggtttgtttc tgcttctgag cattaatgat aatattacga tttctataat
     3361 tgatcagctt accaatagag gatggctaaa cccgcctcga gaacatgaaa tttctaacaa
     3421 gatgttagaa ttacttcaga taaaagcacc agggattttc caccccgtca gttctttatc
     3481 aggaggaaat cagcaaaaag tggtggttgc caaattactt gctgctcgcc caaaaatatt
     3541 aatcatggac gagccaacta agggtgttga tgtgggagca aaagctgcta ttcatcaaat
     3601 catgtctgat ctcgtagcag aaggactggg tattattatg atttcttctg agatgccaga
     3661 aatattggga atgagtgata atatcatcgt tatgcacgaa ggccgaatta ctggtgtttt
     3721 ttctcgttca gaagctactc aggaaaaact tatggaagca gcggtaaaag tcgatcgttt
     3781 aactcaggtt gtgtcgtaaa atttagcttg gatggtgtag acccatgatg gatgaacctg
     3841 ctaaaacaaa aagagaagtc acgttttcca ggctgcttgg attccgggaa ttggggatta
     3901 taattttcat cgtacttata tccgttttga ttggtattcg caatcctcgc tttttaacct
     3961 ggagtaattt tcatgatatt ttgatagata cagcagccct atctgtcctt tctgtgggga
     4021 tgatgttagt tctggtgacc ggtggtattg acctttctat ggaatctgtg ttagctctaa
     4081 cgggaatggt agcgggaatg atggttaaac aaaatcccca gctttctcca ctttttatgt
     4141 taatactggg attattattg ggaggtcttc tgggatctat tactggtttg attgtagcta
     4201 aaggtcgggt ggtgccgatt attgctactt tgagtatgat gtatatttac cggggcatta
     4261 cttttataat cagcggagga aagtggataa atgcctacga aatgtcggat tcttttaagg
     4321 gaatagcgac agaacgtttc ctaggtcttt ctaagctgat ttacattgtt ttagtggtgt
     4381 acgtagtttt ctattatttt gtcaattata ctcatactgg aagaaaaata tatgcggtag
     4441 ggagcaatgt ggaggcggct cgagttatcg gaatcaatac tgaatttatt acctggctgg
     4501 tttatgccat ttctggtgct ctctccggtt tagccggggt gatgtgggta gctcgttatg
     4561 cctcagccca aaatgatact gcttctggat ttgttatgac tattgtagct gcttgcgttt
     4621 taggtggggt tagcattact ggtggtgtag gaactgtatc tggtgtttta ctgggatcta
     4681 tcactgttgg tattattaat aacgcacttc ccatgattag ggtgtctcct ttttggaaga
     4741 tggccttgca gggtcttatc attttagtag cagcgatagt gaatattgct attgctcgaa
     4801 atatggaaaa gtcgcagttg aagaggagaa caatatagat gaaataccgt aattctcttt
     4861 ctaataggaa acttaacatc gttttcaagt gggaatggct tttggttata ttgattattg
     4921 ggatatcaat tgttaatggt cgcctttcac ctcatttctg gagctatacg ggtatcatgg
     4981 actctttagc agtgttctta gaaaaaggtt ttatggttct ttctatgact ctggtattga
     5041 ttatagggga tattgatatt tccgtagctt ctattgttgc tctctcttcg gttttgatgg
     5101 ccatgtctta caagcttggg cttcctatgg cagtcgctat tttagtagcc ttggctacgg
     5161 gagccgggtg tggatatttg aatggcttgt tgatctcgct tgtacctggc ctatcggcta
     5221 ttattgtcac cctggctggt tcctccattt atagaggaat tgcctatatt ttgcttggtg
     5281 atcaggcaat agggggattc ccctcctggt actcttatct ggcctggggg tatgtaggta
     5341 aaaccaatat accttttgtt atggtggtat ttgtggggtg tgccattata tatggacttt
     5401 tgctccatcg cactgcctgg ggaagaaagg tatacgctat gggaattaac ccggtggccg
     5461 ccagattttc tggagtaccg atagcgcaaa tgaaacagat tatttttact cttaatgggt
     5521 taatggcggg gatagcagct atttttctaa cttccaagtt aggtagctca aggcccaata
     5581 ttgccacagg atatgagctc gaggttattg ccattgccgt tttgggggga gttagcccct
     5641 caggaggaaa gggcggcata gttggtgtat gtttagctct tatattgatg cgtttacttc
     5701 gttatggtat ggggctggta aatgttccgg gtcaggtaat gatggtcatc attggaatag
     5761 ttcttgttgg tgtagtaatg cttcctaata tttttagctc tcgaggggga aagagaagaa
     5821 cgaaagagat tcaatgaatt ttatgagtga agttaaatat tcctccggga atatgaaata
     5881 tgaaaagtta aaagaaaacc ttttagcact gggaagagta atagttgctt tttcgggggg
     5941 agtggattct acttttcttc tctgggtgag tagagaaact ctgggagaaa atgtcttagg
     6001 ggtcctggta gatttacctt ttttaccccg ccgagagagg gaagaagcgg aaaaaattgc
     6061 ccgggattta agacttcctc ttaaggtcat agaaatttcg ggagaagatg agcccctcat
     6121 ctggcgca
//
LOCUS       DAOPSS010000092         6095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_10582_length_6095_cov_7.250000, whole genome shotgun sequence.
ACCESSION   DAOPSS010000092 DAOPSS010000000
VERSION     DAOPSS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_6095_cov_7.250000"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    57..288
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            336..1562
                     /locus_tag="PKN31_06115"
     CDS             336..1562
                     /locus_tag="PKN31_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOA99534.1"
                     /translation="MGEKLRTYEEINEKIRQGKAVVVTAEEVIDLVEEKGVEKVAQEV
                     DVVTTGTFGVMCSSGAFFNIGHTKPRMKVSEAYLNGVMAYSGLAAVDFYIGAAQVAQD
                     DPLNKIHPGEFKYGGGHVIQDLVAGKDVLLEAYGYGTDCYPRRKLVTWINLADLNEAY
                     LFNPRNAYQNYNVAVNLSSKTLYTYMGVLLPKLGNANYSSAGQLSPLLNDPFYRVIGV
                     GTRIFLGGGVGYVAWQGTQHNPGVERNERGIPKDGAGTLAVIGDLKGMNANWLWGVSM
                     RGYGCSMAVGLGVPIPILDEETLYYCAVKDEDILAPVIDYSDAYPNGTGEILGYASYA
                     QLRQGKIKIEGKEVPAASLSSYSRAREIALTLKDWIQKGDFTLTQPVLPLPGKDAGAR
                     FHNLPERPVNNGRVGR"
     gene            1567..1977
                     /locus_tag="PKN31_06120"
     CDS             1567..1977
                     /locus_tag="PKN31_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA99535.1"
                     /translation="MNSSQKVVLRFPPELVERPLVCELALQFGLHFNIMRASISPHRE
                     GLMVIEIIGEKKKQEEGLQFLQENGLEVESLSQDIKMVEERCIHCGSCVGVCPTEALY
                     VEKPSYLVKYDETQCIACEMCVLACPTHAMEALL"
     gene            1974..2702
                     /locus_tag="PKN31_06125"
     CDS             1974..2702
                     /locus_tag="PKN31_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOA99536.1"
                     /translation="MTRYVERWYRGYTKAPDLVPFSVQIKESDLLIWAEKNLQDLAYK
                     RLEKERNNLEAYLRYDPDFLHSLAPVAASPFAPPLCQMMAKAAEKAGVGPMASVAGAI
                     NEMVGEELRQESEEIIIENGGDLLVFSREARVVAVYAGEDSPFSFRIGIKLPPGKKWG
                     VATSSGIVGHSLSFGKADAVVVVAESSAVADAWATSLANRVQEKEDIKKVLKFSQQIT
                     DIQGTLIILGEFLGVEGNIQIERL"
     gene            complement(3862..3938)
                     /locus_tag="PKN31_06130"
     tRNA            complement(3862..3938)
                     /locus_tag="PKN31_06130"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3902..3904),aa:Met,seq:cat)
     gene            4035..4205
                     /locus_tag="PKN31_06135"
                     /pseudo
     CDS             4035..4205
                     /locus_tag="PKN31_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            4393..4797
                     /locus_tag="PKN31_06140"
     CDS             4393..4797
                     /locus_tag="PKN31_06140"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOA99537.1"
                     /translation="MIEPYIMFNGRASEVIDFHEKVFHGTNKRVTHWGNAPTNTDYPV
                     DATKKEWIPHSEIELCDTNLSISDCDQVFQTTHFVSLMARLDMPEEVQRIYEELQADG
                     GKVLMEIEPTFYTKMYAWVQDKFGVSWQLICD"
     gene            4827..5165
                     /locus_tag="PKN31_06145"
     CDS             4827..5165
                     /locus_tag="PKN31_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006289930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOA99538.1"
                     /translation="MKPDNQAMLVTFFEAENNRDWATYSVFLSNDVVWELRSGGQTKT
                     ISGKEQYVEYMRSVYERYDNTFVCEEMYVSGNCNRIVAILKNDIGERSCDIFDFDNGL
                     IVREYEFTLE"
     gene            5175..5603
                     /locus_tag="PKN31_06150"
     CDS             5175..5603
                     /locus_tag="PKN31_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019035172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOA99539.1"
                     /translation="MTISDRMPDSIDIYAFFDAVVKQDAVRLRSFFEPDATIVWVNTN
                     EVFTVDEYIRANCEYPGDWRGRIEDVQCYLCFHDYNRITVVAKVWDGKNNASRVVSFI
                     ELGNTENELIQSMVEYWGDVGEPPEWRQKLAIGKRYRDEK"
     gene            5811..6056
                     /locus_tag="PKN31_06155"
     CDS             5811..6056
                     /locus_tag="PKN31_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOA99540.1"
                     /translation="MATAYLHCIKDDVFIDPCCGSGGLLIKSHPRFKEKYGSDRSVEH
                     LEFYGQEILFSTFAIAKINSLYAAWKRFIFLLSDKKH"
BASE COUNT         1766 a         1185 c         1582 g         1562 t
ORIGIN      
        1 ctcgccctat tgacaaacga ggggggaaat tatacaattt agctcacttt aatagaataa
       61 agactgagag caggattagt aaccagggga aggattagag agagcctgga taaggtggga
      121 gtcaggtatt cagaacctgg tgaaatggcc tgggagttga agctgcgaaa ttaagtagtg
      181 gtttacgggt agtctccgtt aaaaagactg cactcttcca gtgtaaaaga ggttggcttg
      241 tgccagcgaa atagggtggt accacgagat taacccctcg tccctcgacg aggggttttt
      301 tgttatataa ggaagaaata gcaggaggtg agaaaatggg agaaaaactc cggacttatg
      361 aagaaatcaa tgaaaaaatc cgacagggta aagcagtagt ggtgactgct gaggaagtaa
      421 ttgacttagt agaagaaaaa ggtgtagaaa aggttgctca agaagtagat gtggtgacca
      481 ctggcacttt tggagtgatg tgctcatcag gagctttttt taatatcggg cacaccaagc
      541 cccgcatgaa agtcagcgaa gcctatctca acggagtaat ggcctactct ggtttagctg
      601 ctgtggattt ctacatcggg gcagcgcagg tagctcagga tgacccgctc aacaaaatcc
      661 atcccggaga atttaagtat ggtggaggac atgtgattca ggatttagtg gcaggaaaag
      721 acgtccttct cgaggcctat ggttatggaa cagattgcta tccccgccga aagttagtaa
      781 cctggatcaa tttggcggac ttaaacgaag cctacctttt taacccccgc aatgcttacc
      841 agaactacaa cgtggcggtg aacctctctt ctaaaaccct gtatacctat atgggggtct
      901 tgctccccaa attaggcaat gccaattatt cttccgccgg acaactttct cctctgctta
      961 atgacccttt ttatcgagta atcggggtag gaacacggat ttttctggga ggaggagtgg
     1021 gatatgtagc ctggcagggg acgcaacaca accctggagt agagagaaac gaacgaggta
     1081 tacccaaaga tggagcagga accctggcgg ttattggaga tttaaaagga atgaatgcta
     1141 actggttgtg gggagtaagc atgagaggct atggttgctc tatggccgtg ggtctgggag
     1201 tccctattcc tatcctagat gaagaaaccc tttattattg cgcagtaaag gatgaagata
     1261 tactggctcc tgttatagat tacagcgacg cttatcccaa cgggacaggg gagatattgg
     1321 gttatgcgag ctatgcccag ttgcgccagg gaaagataaa gatagaaggg aaagaagttc
     1381 ctgctgcttc tctatccagc tactctcgag cccgggaaat agccctgact ctgaaagatt
     1441 ggattcaaaa aggagatttc actttaactc aaccagttct acctcttccg gggaaagatg
     1501 caggagctcg tttccacaat cttcccgaaa gaccggtaaa taatggaaga gtggggaggt
     1561 gaaaatatga actcttccca gaaagtagtt ttgcggtttc ctcctgagct ggtagagcga
     1621 cctctcgtgt gtgagctggc tcttcagttc gggctgcatt tcaacattat gcgcgcttct
     1681 atttcccccc atcgggaggg acttatggtg atagaaataa ttggagaaaa gaaaaaacaa
     1741 gaagaaggac tgcaattttt gcaggaaaac ggtttagagg ttgaatctct gagccaggat
     1801 ataaaaatgg tggaggagcg ctgcattcac tgcggctctt gtgtaggagt ttgccccacc
     1861 gaggctcttt atgtggaaaa accttcttat ttagtaaaat atgatgaaac tcaatgtatt
     1921 gcctgtgaaa tgtgtgtttt agcctgtccc actcacgcta tggaggctct tttgtgacca
     1981 gatacgtaga acggtggtat cgtggatata cgaaggcgcc ggatttagtg cccttttctg
     2041 tgcagattaa agagagcgac cttttaattt gggcagagaa aaatctgcag gacctggctt
     2101 ataaaaggtt agaaaaagag agaaacaact tagaggccta tcttcgctat gaccctgatt
     2161 ttttgcattc tttagctccg gttgctgctt ctccttttgc tcctcccctg tgccagatga
     2221 tggcaaaagc agcagaaaaa gcaggagtag gtcctatggc ttctgttgcc ggagcaataa
     2281 atgagatggt gggagaagag ctccggcaag aaagcgagga gataataatt gaaaacgggg
     2341 gagatttact ggtcttttct cgggaggctc gggttgtggc agtgtatgcc ggggaagatt
     2401 ctcctttcag cttccggata ggcattaaat tgcctccggg caaaaaatgg ggggtggcta
     2461 cttcttcagg aatagttggc cattctttaa gcttcggtaa agcagatgct gtggttgtgg
     2521 tagctgaatc atcagctgtg gctgatgcct gggctaccag cctggctaac cgggtgcaag
     2581 aaaaagaaga cataaagaag gtattaaaat tttctcaaca aattactgat attcaaggga
     2641 ctttaataat tctgggagaa tttttaggag tggagggaaa tattcagata gaaagacttt
     2701 aaactctttt ttgtggtaaa atttaagaaa atacctggcg ggggataaag aaaatggctc
     2761 tggtaatcat ctttgttttg gcgcttcttg ctatttatgt ctcgggaata gagagaacaa
     2821 agtccatctc tcctgaggtg cgcgaattgt ttcaccaggg tttctctctt gtccgggata
     2881 aaagatatac agaggctctg gaaatttatg acttcatcat aaacttgttg cccgacgatc
     2941 ctacagctta tgcccagcga ggtaacgttt actactattt aggagaaata gataaagcca
     3001 tcgaagatta ttctaaagcc attgagctga agcccgattt tgccgaagct tacaacaatc
     3061 ggggaaatgc ttatctatca aaaggagaaa tggaacgagg agtgagagac ctgaccaggg
     3121 cagtggagct gagtcctcag ttgaaagaag cgtttttcaa tcggggaata gctcgctcag
     3181 aagaaggaaa gtataaggaa gcattggaag atttcagcca ggtaactcga attgaccctc
     3241 aagaggccga cgtatatctt gcccgagccc gtctcttgtt aaaattaaag acttattcca
     3301 accaggttct ggctgatatc aatcattttc tggaatttta cccttctcat cctcaggctt
     3361 attttctgag aggaatatgt ctttcccgcc aggaagatta ttatcaagcc atccaggatt
     3421 tttcccgggc cttagagttt aaaagtgact tccctatggc ttactactac agaggaattg
     3481 cccggattaa acaaggggaa tatgcagaaa ccatagaaga ctttaccgct gttctaaatt
     3541 tagagccgga attttaccct gctcttctgg gaaggggaat agtttattac cagaagaaag
     3601 attatgaact ggctaaagaa gacctcgacc gagcgataaa actcaacccc cggttctata
     3661 aagcttacgg cttcagagct atggtttacc gggcactggg gaaagaggaa gaggcagaaa
     3721 aagactggga agagttttta aaaggaaaca tcatcgatag tttagccgaa gaaaatttag
     3781 aagaagaagc aaatgacctc ttttcctggg gagagaaaat acttctctgg ggcgaaaacg
     3841 aagagctgaa ggaagaagga atggtagcgg gggtcggatt cgaaccgacg acctccgggt
     3901 tatgagcccg acgagcttcc tgactgctct accccgcgcc gtttaatgtt cgcaaaatgc
     3961 ggacagtatt aatattaaac tattttttta ttttgtcaag ttttcgaggg ctctatgcaa
     4021 aaggaggttg aaacatgccc ggaggtaagc tcgacgtaaa aacactggag agctggctgt
     4081 gggaagctgc ctgtaaaatt agaggcccaa ttgatgcgcc taagtataaa gactacattt
     4141 tgccgcttat attcttaaaa aggctatctg atgtgtttga cgatgagatg aatgagctgg
     4201 caaagtaatt tggtcctaga aaattagttg aagagattgt aactgatgat cattgcgtag
     4261 tcacttctat actgaacctg taaatggccg gacacctgaa agtgtggggg ttgtttagga
     4321 gatgcatcta gccgaggaga ttttacacac aaatatggac ttgatcgaaa aaggacaaag
     4381 gaggaaaccg caatgattga accttacatt atgttcaacg ggcgggcttc ggaagtcatc
     4441 gactttcacg agaaagtctt ccatggcacg aacaagcgtg tcacacattg gggcaacgct
     4501 ccgactaaca cggactaccc cgtggacgct acgaaaaagg agtggatacc tcatagcgaa
     4561 atcgaactgt gcgacacgaa cctttccatc tcggactgcg accaagtatt ccagaccacg
     4621 cactttgtgt cgctaatggc gcggctcgac atgccagagg aagtacagcg catatacgaa
     4681 gaactgcaag ccgacggcgg caaggtgctg atggaaatcg agccgacgtt ctacaccaag
     4741 atgtacgctt gggtacagga caaattcggt gtgagttggc agttaatctg cgattgagta
     4801 aaggctcgaa agcgaggaaa cggaaaatga agccagacaa ccaagctatg cttgtgacat
     4861 tctttgaagc tgaaaataac cgtgactggg ctacatacag tgtgttcctt tcgaacgatg
     4921 ttgtttggga gttgcgctcc ggtggtcaga caaagaccat aagcggcaaa gaacaatatg
     4981 tcgagtatat gcggtctgtt tatgagcgtt atgacaacac ctttgtttgc gaggagatgt
     5041 atgtcagcgg taactgcaat cgaatcgtgg ctatcttaaa aaatgacatt ggtgagaggt
     5101 catgcgatat tttcgatttt gataatggct taatcgtgcg agaatacgag tttactttgg
     5161 agtaggaggc gaagatgacg attagcgata gaatgcctga cagcattgac atatatgcct
     5221 tctttgatgc cgtagtcaaa caggacgctg tgaggctacg ttcatttttt gaacccgacg
     5281 caactattgt ctgggtgaac accaatgagg tgttcacggt cgacgagtac atccgggcta
     5341 actgtgaata tcctggcgat tggcgggggc gcattgagga cgttcaatgc tacttgtgtt
     5401 ttcacgacta taaccggatt accgttgtgg caaaagtatg ggacggaaaa aacaacgcat
     5461 cgagggtggt atcgttcata gaacttggta acaccgaaaa cgaactcata cagtcgatgg
     5521 ttgaatactg gggtgatgtt ggcgagccgc ctgaatggcg gcagaagctg gccatcggca
     5581 agcgttaccg agacgaaaaa taaatctttt aattttgcct gaacctttta atttttcctc
     5641 ggaagcgatc gagagggcgt gcaaaaatct tgacagctaa tacagcggcg gtgcggacaa
     5701 tgccgctgaa cacaaatttc ggacaaataa aaaggcccct ttggttacgg cgtgaccgca
     5761 gaaatccctt atatcaagcg gttttcgccc ctctgtcctt aaacgggaag atggcgaccg
     5821 cttatttgca ttgtatcaaa gacgacgttt ttatagatcc ctgctgcggt tctggaggtt
     5881 tgttgatcaa atctcatccc cgatttaaag aaaaatacgg aagtgatcga agcgtcgaac
     5941 atttggaatt ttacggccaa gaaatactat tttccacatt tgccatagcc aagattaaca
     6001 gtttatacgc ggcatggaag cgttttattt ttttgctatc tgataagaag cattgagtta
     6061 acagtgggaa ggagaggtga tgaagcacct tatct
//
LOCUS       DAOPSS010000093         6084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_10609_length_6084_cov_6.153425, whole genome shotgun sequence.
ACCESSION   DAOPSS010000093 DAOPSS010000000
VERSION     DAOPSS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10609_length_6084_cov_6.153425"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1375)
                     /locus_tag="PKN31_06160"
     CDS             complement(65..1375)
                     /locus_tag="PKN31_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA99541.1"
                     /translation="MREGSNLEFKETWRDEYLKTLAAFANSSGGEMLIGVDDKGRSVG
                     AKNSERLLEELPNKIRDKLGLITSVEVEDFEGREIVKVAVKPSFVPVSYNGKFYVRTG
                     STVQELKGNELIQFILKRQNLSWDALPSKAGVEEIDTETVERFKTLATKRLPDISEAD
                     SVEKVLSNLDLIENGKLRNAGVLLFGKNPQKHLMISEARVGKFKSDIDILDTVTAKGN
                     LFKQLDILFDAVKKHLNVKYVIKGQLEREDVWDYPLDAVREAIINALIHRDYLDNADI
                     QIRVYDDKIRFWNPGLLPEGITIDMLRTDHESRPRNKLLAMIFYFAGLIERWGSGTKR
                     MIDLCRAQELPEPEFREEGAGFAVIFYKDIYTEENLRKMGLNERQVKAVMYVKEKGKI
                     AIADYKEISPDVSKKTLYRDLQGLVKKEILHEVGDKKGRRYELR"
     gene            complement(1362..3851)
                     /locus_tag="PKN31_06165"
     CDS             complement(1362..3851)
                     /locus_tag="PKN31_06165"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOA99542.1"
                     /translation="MNNGRITLRQLETHLFQAADILRGKMDASEYKEYIFGMLFLKRV
                     SDVFKVKRQELKEKYKKSPELSKEQIDELLEDPDSYGDTFFVPERARWKNILNLKEDV
                     GNQLNKALAAIEDGNPELDGVLRHIDFNAVKGKTRLKDQQLVDLIHHFNKYRLTNDDF
                     EFPDLLGVAYEYLLKQFADSAGKKGGEFYTPPHVKKLMVRIVKPQEGMTIYDPTVGSG
                     GFLIESRQYVEEQGKNPQNLGLYGQELNGLTWSICKMNMILHDILDAHIENEDTLTNP
                     QFQENGYIKRFDRILANPPFSQNYSRANMQFPERFKYGFTPETGKKGDLMFLQHMIAS
                     LKDNGIMATVMPHGVLFRGGQEKIIREGIVRDDLVEAIIGLPPKLFYNVGIPACIIVI
                     NKNKPEYLKSKILFINADREYGEGRNQNYLRPEDMEKIVTVFDKKLEISKYSRLVDIG
                     EIEENDFNLNIRRYVDNSPDPEIEDVHAHLLSGVPKREVELYREEFLKFGLSLYLLLK
                     EKNEKYLEFREDIAEKSRIKEIIESADTIKDTALKHRKELQEWWNEVKTEIEGFHRDN
                     DLWGFRNRAMQRLKERLLPLGSFDEFKISGIFVNWWEELRYDFKTVESLGWSKNLIED
                     ERIKEKFFEAEIEEIKRLEGKIAELEGELNDLLEGIEDWDEEEQGDKTANKVKEYLEE
                     VTKDLKASQSESAAKEAAKWQRLTLEIEDKERELKKLRKKLKDKEQGLEEKTKRKRES
                     LSEEETKELLLDKFYNLINEQLTRYLNTEKKEIIKIFENLWDKYKVSLLELNEERNRE
                     VKKLNEFLENLGYYRELGIEEQESGAYYEGR"
     gene            4109..4270
                     /locus_tag="PKN31_06170"
     CDS             4109..4270
                     /locus_tag="PKN31_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99543.1"
                     /translation="MRMKKSILWENFPLLLEERKERKNSAIPKIFIGNPSRPHSRDFL
                     SGIHLSRHA"
     gene            4378..4587
                     /locus_tag="PKN31_06175"
     CDS             4378..4587
                     /locus_tag="PKN31_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99544.1"
                     /translation="MTRKSLGWRIRKGLIRGDSFSFIILYPLAIREEFSMVVDKELLL
                     VAEKSMNIGGEKSPPMFMLLIALIA"
     gene            complement(4577..>6084)
                     /locus_tag="PKN31_06180"
     CDS             complement(4577..>6084)
                     /locus_tag="PKN31_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA99545.1"
                     /translation="LRCIGATTLDEYRKYIEKDPALERRFQPVYVGEPSTEETKEILK
                     GLRDKYEAHHRVKISDEAIEAASELSSKYITERFLPDKAIDLMDEAASKKRIEIESIP
                     SEIKILEDELQKLAKEGMAAVQAQEYEKAAALRDEAEELQKRYQEEKERWMRERELSE
                     IVSEKDIAEVVSAWTGIPMAKLVEEEKERLLRMEEELHKRVVGQDEAVIAVAEAIRRS
                     RAGIAEGRRPMGSFLFLGPTGVGKTELAKSLADFLFGDENAILRIDMSEYMEKHSVSR
                     LIGAPPGYVGYEEGGQLTEVVRRRPYQVILFDEIEKAHPDVFNILLQVLDDGRLTDGQ
                     GRTVDFTNTVIIMTSNLGSAYFRGIDPEKDKEALRRAKEKVLEEVRNHFKPEFLNRID
                     EIIVFDPLGREEIKKIVDLMINRLRNRLQAQGMDIVLTPEVKDYLAQAGYDPDYGARP
                     LRRLIQREIESPLSGELIRGTFQPGDVIEVYLEDGEIKFRRKERIEEEVKL"
BASE COUNT         1572 a         1470 c          962 g         2080 t
ORIGIN      
        1 aattcctaat tttatatgcc caataaatgt ctaatatttg tccaatatgt ccaacaatcg
       61 tctattatcg caattcatac cttctaccct ttttgtctcc aacctcgtga agaatttcct
      121 tcttgaccaa cccttgtaaa tctctataaa gtgttttttt agaaacatcc ggagatattt
      181 ctttataatc ggcaatggca atcttccctt tttctttcac atacataact gctttaactt
      241 gtctctcatt cagccccatc tttcttaaat tttcttctgt ataaatatct ttataaaaga
      301 ttactgcaaa ccccgctcct tcttccctga actctggctc tggaagctct tgagcacggc
      361 acaggtctat cattcgcttc gttccactcc cccatcgctc tattagcccc gcaaagtaga
      421 aaatcattgc cagaagctta tttctcggtc gtgattcgtg gtcagttctt aacatatcaa
      481 tagttattcc ttctggaagc agcccgggat tccagaatcg tattttgtca tcataaactc
      541 gtatctgaat atctgcattg tcaagatagt ctctgtgtat cagagcatta ataatggctt
      601 cccttacagc gtcaagagga taatcccata catcttctct ttcaagctgt ccttttatca
      661 cgtatttgac attaagatgt tttttgacag catcaaaaag aatatcaagc tgtttaaaga
      721 gattgccttt tgctgtaacc gtatccaata tatcaatatc cgatttaaac ttacctacac
      781 gagcctcaga tatcataagg tgtttttggg ggttcttgcc aaagagcaga acacctgcat
      841 ttctcagttt gccattctca ataagatcaa gattagagag aactttctct acagagtcag
      901 cttctgatat gtcggggagc ctctttgttg ccagtgtttt aaatctctca acggtctctg
      961 tatcaatctc ttccactcct gctttagatg gtaaagcatc ccagcttagg ttttgcctct
     1021 ttagtataaa ctggataagc tcattaccct tgagttcctg aactgtggag cctgttctta
     1081 cataaaactt tccattgtaa gatactggca caaaagaggg tttaacggca actttaacaa
     1141 tctccctacc ttcaaagtct tcaacttcaa ctgatgtaat taagccaagt ttgtcccgta
     1201 tcttatttgg tagctcctcc aaaagccgct cactattttt cgctcctaca gaacgcccct
     1261 tgtcatccac cccaataagc atttcccctc cgctactatt agcaaatgca gctaaagttt
     1321 ttaaatattc gtccctccag gtttctttaa actcaaggtt actaccttcc ctcataataa
     1381 gctcctgatt cctgttcttc aattcctaat tccctatagt atcccagatt ttccagaaat
     1441 tcattcagct ttttaacttc tctgtttctt tcctcattta gctccagaag ggacacttta
     1501 tatttatccc ataggttttc aaaaatcttt atgatttctt ttttctcagt atttaagtat
     1561 ctcgtgagtt gctcattaat gaggttataa aacttatcca gaagaagttc tttggtctct
     1621 tcttcactta acgactccct tttccttttt gttttctctt cgagtccctg ttctttatcc
     1681 tttaattttt ttcttaactt ctttagttct ctctctttat cctcaatttc taaagttagt
     1741 ctttgccatt ttgcagcttc ttttgctgct gactcgctct ggctcgcttt caggtctttt
     1801 gtgacttctt ccagatactc cttaacctta tttgctgttt tatcaccttg ctcttcctca
     1861 tcccagtctt caatcccttc taagaggtca tttaattctc cttccagttc cgctattttg
     1921 ccctccagtc tcttgatttc ttctatctca gcttcaaaaa acttctcttt aattctttcg
     1981 tcttcgataa gatttttgct ccagcccaaa gattctacag ttttaaagtc ataccttagc
     2041 tcttcccacc agttcacaaa tatgccagat attttaaact catcaaaaga acccagaggc
     2101 aaaagcctct ctttcagcct ttgcatcgct ctatttctga atccccacaa atcgttatct
     2161 ctgtgaaacc cctcaatttc agtttttact tcattccacc attcctgtaa ttcttttcta
     2221 tgctttaagg ctgtgtcttt tatagtatca gcactttcaa taatttcttt gattctactc
     2281 ttctctgcaa tatcctctct aaactccaga tatttttcgt ttttttcctt caagagaaga
     2341 tagagagaaa gaccaaactt tagaaattct tccctgtaga gctcgacttc cctttttgga
     2401 acaccggata agaggtgagc atggacatcc tcaatttcag gatcggggga gttatctacg
     2461 tatcttctga tattcaaatt aaaatcgttt tcttcaattt ctccaatatc tacgagcctt
     2521 gaatattttg aaatctccag cttcttatca aatactgtca caatcttttc catatcttcc
     2581 ggtcgcaaat aattttgatt tcttccctct ccatattctc tatctgcatt aatgaacaga
     2641 attttacttt ttaaatattc cggtttattt ttgttgatga caattatgca ggcaggaata
     2701 cctacattat agaagagctt gggaggcaaa cctataatgg cttcaactaa atcatctctc
     2761 acaattccct cccttataat cttctcctgt cctcctctaa ataacacccc gtgaggcatc
     2821 acggttgcca taattccatt atctttcaga ctggcaatca tatgctgtaa aaacatcaag
     2881 tcgccttttt tacctgtctc cggggtaaaa ccgtatttga atctttctgg aaactgcata
     2941 tttgcccgac tataattctg agaaaaggga ggattggcca aaatcctatc aaaacgcttt
     3001 atgtatccat tctcctggaa ttgagggttg gttaaagtat cttcattttc tatgtgtgca
     3061 tccagaatgt catgaagaat catattcatc ttacatattg accaggtcag tccgttaagc
     3121 tcctgcccat agaggcctaa attttgaggg ttcttccctt gctcttcaac atattgcctg
     3181 gactcgataa gaaaaccgcc tgagccaacc gtagggtcat aaatcgtcat accttcctga
     3241 ggtttaacga ttctcaccat taattttttg acatgaggag gggtgtaaaa ttctcctccc
     3301 tttttccctg ccgaatcagc aaattgttta agaaggtact cataagcaac accaagcaaa
     3361 tctggaaact caaaatcatc attcgtaagc ctgtatttat tgaagtgatg tatgaggtca
     3421 acgagttgct gatctttgag gcgagttttc cccttaaccg cattaaaatc aatatgcctt
     3481 aaaacgccat cgagctctgg atttccatct tctatcgctg ccaacgcctt atttaactga
     3541 ttaccaacat cttcttttaa gttaagaata tttttccatc tggctctttc gggaacaaag
     3601 aaagtatctc cgtaggaatc aggatcttct aagagctcat caatctgttc cttggaaagt
     3661 tccggggatt ttttatattt ttccttcagt tcctgccttt ttaccttaaa cacatctgaa
     3721 acacgtttta aaaacagcat accaaaaata tattctttat attcagaggc atccatcttg
     3781 ccccggagaa tgtcagcagc ctggaaaaga tgagtttcca gctgtcttaa ggttattctt
     3841 ccgttattca tagcgtcatc cttaaacttt caatcctttt aaagcagatt tggctatttc
     3901 gcccaacccg gtttagttta tatcttaact tttgtaatgc ttcctcttca ggacttcagg
     3961 tttttatcaa aaaaccactc ttttagagta ttctcctata aaaaactctc cctgtaaagg
     4021 taacctttta acttgaggac tctggagagg gataaaatat aaactcctct gatggttctc
     4081 tcctatctct tgggggagga ataagggaat gaggatgaaa aaaagtattt tatgggagaa
     4141 ttttcctctt cttctcgaag agaggaaaga gaggaagaac tctgctattc ccaagatttt
     4201 tatcggtaat ccatcccgcc ctcattcccg agatttttta tcgggaatcc acctctctcg
     4261 tcatgcctga aatcttttat caggcatcca aatgctttta aaactgtgga cccctgatag
     4321 aaccattcag gggtgacgaa tttggggatg atagagacat tcaggggcag gctaaaaatg
     4381 actcgaaagt cgctggggtg gcgaataaga aaggggttaa taaggggaga ttctttctcc
     4441 tttataattc tctatcctct tgcgataaga gaggagttct ccatggtggt ggataaggag
     4501 ctccttttag tagcggaaaa aagcatgaac attgggggag aaaaatctcc cccaatgttc
     4561 atgcttttaa tagcacttat agcttaactt cttcctctat tctctccttg cggcgaaatt
     4621 ttatttctcc atcttctaaa taaacctcta taacgtcgcc aggctggaaa gtaccccgaa
     4681 tcaactctcc agaaagaggg ctttctatct cccgttgaat cagacggcga agaggtcgag
     4741 ccccatagtc agggtcataa cctgcttgag ccagataatc ttttacctcc ggggtaagga
     4801 ctatatccat tccttgagct tgaagccgat ttcgcaaacg atttatcatc aaatccacga
     4861 ttttctttat ttcttctcta cctaatggat caaatacgat tatttcatca atcctgttca
     4921 aaaattccgg tttaaaatgg tttcgcactt cttctaaaac tttctcttta gcccggcgca
     4981 aagcctcttt atctttttcc ggatctatcc cccggaagta agcacttcct aaattagaag
     5041 tcataatgat aactgtattt gtaaaatcca ccgttcttcc ctggccatca gttagtcttc
     5101 catcgtccag tacctgcagc aagatattaa aaacatcagg atgggctttt tctatctcat
     5161 caaagagaat tacctgataa ggccttcttc ttactacttc ggttagttgc cctccctctt
     5221 catagcccac atatcccgga ggagcaccaa tcaagcggga gacagaatgt ttttccatat
     5281 attcagacat atcaatacgt aaaatggcat tctcatcacc aaacagaaaa tcagccaaag
     5341 atttagctaa ctcggttttg cccaccccgg tagggccaag aaagaggaaa gaacccatag
     5401 gccttcttcc ttcagcaata cctgccctgg agcgacgaat agcttctgct accgctataa
     5461 ctgcttcatc ctgtcccact actcttttgt gcaactcttc ttccatgcgt agcaatctct
     5521 ctttttcctc ttccaccaat ttagccatag gtatacccgt ccaggcagat accacctcag
     5581 cgatgtcctt ttcgctcact atttcactga gctctcgttc cctcatccat cgttcttttt
     5641 cttcttgata tctcttctgt agctcttctg cctcatctcg cagagcagca gccttttcat
     5701 actcctgggc ctgcactgct gccatgcctt ccttagccaa tttttggagt tcgtcttcta
     5761 agatttttat ttccgaaggg atactctcta tttctattct ctttttactg gccgcttcat
     5821 ccatcaaatc tatggcttta tcgggtaaaa atcgttcagt aatatattta ctggatagct
     5881 cagaagcagc ttctatagcc tcatcactga ttttcacccg gtgatgagct tcgtatttgt
     5941 cacgcaaacc ttttagtatc tctttggtct cttctgtaga tggctctccc acatataccg
     6001 gctggaagcg gcgctctaag gccgggtctt tttcaatgta ttttcgatac tcatccagag
     6061 tggtggctcc aatacagcgc aatt
//
LOCUS       DAOPSS010000094         5960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_10873_length_5960_cov_5.890771, whole genome shotgun sequence.
ACCESSION   DAOPSS010000094 DAOPSS010000000
VERSION     DAOPSS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10873_length_5960_cov_5.890771"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKN31_06185"
     CDS             <1..746
                     /locus_tag="PKN31_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99546.1"
                     /translation="SLWALSVNNERVNEGLVSRSPQLVVSYGESPGEGFNWKSTFSWG
                     YFEEDGSSTWRKDLYLNAGWKGKEGGLELFFWNIDLTGGQIIPSWGSKIWWEKRVFPK
                     WDLSLSYQFSEAQGESPFSFDPGDQKIFSLDSCWGDYEESFLRLQGDYNLNEGNWEEL
                     TAGIGLGNEDFSLGVEGVYSFELGEWKEERYFVRKRIEDCITLEASWYEPEGSLFLSL
                     NLSGLDTEKKAEGLFDEEEEFNPFELKRD"
     gene            761..1192
                     /locus_tag="PKN31_06190"
     CDS             761..1192
                     /locus_tag="PKN31_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOA99547.1"
                     /translation="MEKRLYRSRTDRMLGGVCGGIAEYFGVDPTLIRLLAVVLIVAGG
                     AAIIAYIIAWIVIPEEPKNLSIEEKAEKENVTIPGEKSEERRKDVELIAWILIFVGLI
                     WFCLNLFSLWLPSRFFVRLVFPVALVIVGILLLWERKKEER"
     gene            complement(1546..2559)
                     /locus_tag="PKN31_06195"
     CDS             complement(1546..2559)
                     /locus_tag="PKN31_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOA99548.1"
                     /translation="MPSIKEVARLAGVSITTVSRVINNKGSVKKETAEKVWEAVHLLN
                     YQPNLLARSLRSQQSRLLGLLVPDIESPVFARLAKHLEEIASKKGYSLIFCNTNDDPE
                     KEKSYLEILIRRQVEGIIFSRVSDESLLFKTPQLSKIPYIVLDRTLEKEEAPTIKLDN
                     YTAGVLAASHLLNLGHKKLACLAGPLKIKICRERFYGFLDTLEKSGIGLREELIEEGD
                     FKIEEGRKAMNRILSFSPLEQPTAVFSMNDLMAIGAIQAIRERGLSVPGDISIIGLDN
                     IPLCNVVSPPLTTIAQPFDEMAKEGLSLLLKLIEGRKIRKTIIVVQPQLVIRSSTAPP
                     RKR"
     gene            2811..4013
                     /locus_tag="PKN31_06200"
     CDS             2811..4013
                     /locus_tag="PKN31_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99549.1"
                     /translation="MSNPASLLNIALEKGQGVFRLFPNWVPRAFCIPGKRLKLHPDDY
                     FAFGVHRGGIDERWFASTVRADNGPETLPDEGLSYIYIEDGPRVEKILLKDAIELMGK
                     DILGKEIMEKYGGWTMFAKFFDNMDPLPHHLHLDDEKAARVGQKGKPEGYYFPRQLNN
                     HGGYFPYTFFGLNPGTTRDDILQCLKNWDKGDNGILYLSRAYKLQLGTGWNVPPGVLH
                     APGSLLTYEPQRASDVFAMFQNIVWDRYLSWDMLVKDVPPEHKKDLDYIVDLIDWELN
                     VDPDFYNQYFLKPKPVKAIEEMEKEGYEEYWIAYNTEYFSAKELTIFPRTKVTIREDA
                     AYGLIVIEGRGKFGILEVETPTMIRFGQMTKDELFVTQEAAVNGITIVNESENENLVI
                     LKHFGPKS"
     gene            4015..5523
                     /locus_tag="PKN31_06205"
     CDS             4015..5523
                     /locus_tag="PKN31_06205"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA99550.1"
                     /translation="MEEVILEFDKLTKKFPGVVALDEVSFDIFRGEVHALVGENGAGK
                     STLIKIVTGVYQKTSGEVFYQGKSINFRSPHDALQNGIAAIYQEFNLIPALTVAENIF
                     MGHHFVRGKFVDWKKMEEEARKLLDFLEVDIDVNSRVRDLGVAKKQVIEIAKALSHNA
                     KVLIMDEPTAALAQKEIKTLFRIIRFLKESGVTIIYISHRLEEVYEICDRVTVLRDGK
                     FVATKPVKELKVEELIRMMVGREVGERFPRVSHTIGEEVLRVEGLTRPGVLENINISL
                     HQREILGIAGMVGSGRTELLRAIYGVDPWEEGKIFIRGEEVKITSPPEAISLGIALLP
                     EERKTQGLILLLSVLDNLGLPILPNIANAGFIDEDRLKQIGEEMVEAMNIKTPSLYQR
                     VMYLSGGNQQKVVLGKWFARKCDIYLFDEPTRGIDVGAKVEIYQLMNRLVSEGAAIIM
                     VSSELPEILAMSDRILVMREGKIAGEVNREEANQELILNLALGGKNHYARTP"
     gene            5507..>5960
                     /locus_tag="PKN31_06210"
     CDS             5507..>5960
                     /locus_tag="PKN31_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99551.1"
                     /translation="MPEPLETKKEKVSLVERFFILWDRIGILLAFIVVCILFGILTPV
                     FFNPLNILNVIRQVSIIGVIAVGMTFVILLGGIDLSVGSVVAFTGIIAAGFQVKWGGS
                     LFLSIVIPLLIGGGIGFLNGFISTKGGLHPFIVTLGTMSIFRGATLLVA"
BASE COUNT         1850 a          968 c         1309 g         1833 t
ORIGIN      
        1 tttctctttg ggctttaagt gtgaataacg aaagagtaaa tgaaggtctt gtttctcgtt
       61 ctccccagct ggtggtttct tatggagaaa gtccagggga aggttttaat tggaaaagta
      121 cttttagttg gggatatttt gaagaagatg ggtcttctac ctggagaaaa gacctttatt
      181 taaatgctgg atggaaggga aaggagggag gactagaact ctttttctgg aatatagatc
      241 ttactggagg gcaaataata cccagctggg gaagtaaaat atggtgggaa aagagagttt
      301 tcccaaaatg ggacctttct ctctcctatc agttcagcga agcacaagga gaaagccctt
      361 tttcttttga tcccggggat caaaagattt tttccttgga ttcctgctgg ggagattacg
      421 aagaaagctt tctacgatta caaggagatt ataatctcaa tgaaggaaat tgggaagagt
      481 taactgccgg aataggatta ggaaatgaag acttttcctt gggagtggag ggagtttatt
      541 cttttgagct gggagaatgg aaagaagaaa ggtattttgt acgtaaaaga atagaagatt
      601 gcataactct ggaggcttcc tggtatgagc cggagggtag cttattttta tctctgaatc
      661 tatctggttt agatacagaa aaaaaagctg aaggtctttt cgatgaagaa gaagagttta
      721 atccttttga attgaaaaga gattagggga gggaattgat ttggaaaaga gactatatcg
      781 ttctcgaaca gacaggatgt taggaggagt atgtggaggg attgctgagt atttcggtgt
      841 tgatcccact ttaataagac ttttagctgt agtccttatt gtggctggag gagcagccat
      901 aattgcctat attatagctt ggattgttat tcccgaggag cctaaaaatc tttcaataga
      961 agagaaagca gaaaaagaaa atgtaactat tcccggggag aaaagtgaag agaggagaaa
     1021 agatgtagaa cttattgcct ggatcttaat ctttgtgggg ttgatatggt tttgccttaa
     1081 ccttttctct ttatggttgc cttctcgctt ttttgtccgt ctggtctttc cagttgctct
     1141 ggttatagtt ggtattttgc tactgtggga aagaaaaaag gaggagcgat gaagatcata
     1201 gataaagagg ctagagatac tttaaaatcg gtctaaagag ctctaaaaat tttaccattg
     1261 ataaaaattt cagaatctct caaaaaagtg gagaaaccat ccactagaat tgttaagcag
     1321 taatgaaatg gtgtttataa gaaatagaaa gtagtcaatc ttagctttac agacattaat
     1381 ctacgttgat aaaagggttt tatctgcatt ctactggtat tggtaaagct attttggcct
     1441 ttaaccattt tccaaatatt ctcattcttc gggttataat tttcataatt agcactaaaa
     1501 gataaagttg ataaatcgat tgctcttgaa taacaagaga acaacttatc gctttctagg
     1561 aggagcagta gaacttctta tcactaactg aggttgcact actattattg ttttacgaat
     1621 ttttctgccc tcaataagct tcaaaagaag gctcaaccct tctttagcca tctcatcaaa
     1681 gggttgagct atagtagtta gagggggaga aaccacatta caaagaggaa tattgtctaa
     1741 gcctatgata gaaatatctc cagggacaga taatcctctt tctcttattg cttgtatagc
     1801 tccgatggcc atcaaatcat tcatagaaaa aacagctgtg ggttgttcta gaggagaaaa
     1861 actgagaatt ctattcatgg ctttcctccc ttcttctatt ttaaagtctc cttcttcgat
     1921 aagctcttcc cgtaagccta taccgctttt ctccaaggta tccagaaatc catagaatct
     1981 ttctcgacag attttaattt tcaggggccc ggctaaacaa gcaagctttt tgtgacccaa
     2041 gttcaaaaga tgagatgcag ctaggactcc ggctgtgtag ttatctaatt ttatagtagg
     2101 agcctcttct ttttctagag tcctatctaa aactatgtaa ggtattttag ataactgagg
     2161 ggttttaaac aataaacttt catctgacac ccgggaaaag attattccct ccacttggcg
     2221 ccgaattagg atctcaaggt aacttttttc cttctccgga tcatcgttag tattacagaa
     2281 aatcaagctg taaccttttt tagaggctat ttcttctaaa tgttttgcta atcgagcaaa
     2341 aaccggactt tcgatatcag gaacgagaag gccaagtaat ctagattgct gactacgcaa
     2401 gcttcgagct aagagattag gctggtaatt aagcaaatga accgcttccc aaactttttc
     2461 tgcagtctcc ttttttactg accccttatt gtttattact cgagaaactg tggtgataga
     2521 aactcccgct aagcgagcta cttctttaat tgaaggcaat ctttttctcc ttttttgaaa
     2581 acgtttttca taaaataaga catttacata ggttacatat tatgtaccaa ttaatatata
     2641 gtaaaattag tcattgacaa agtcaaatta atttgttaac gttttctaaa aacgttaaac
     2701 taaataaaaa aagaaggtaa tttgtcagag aatactatag attaataaat ctcttgtttt
     2761 atgttaaact tatgatgtgt ttaaaaatgc cacaataagg agatgaaaca atgtctaatc
     2821 cagcctcact tttgaatatt gccttagaaa aagggcaggg agtttttcgt ttgtttccta
     2881 attgggttcc tcgagcattt tgtatcccgg gaaaaaggtt aaaacttcat cctgatgatt
     2941 attttgcttt tggcgttcac cgaggaggta ttgatgaacg ttggtttgcc tctacagtta
     3001 gagctgacaa tggtccagaa actttacctg atgaggggtt aagctatatt tatatcgaag
     3061 acggtcctcg ggtagaaaaa atccttctca aagatgctat agagttgatg ggtaaagata
     3121 ttttaggtaa agagataatg gaaaagtatg gcggttggac tatgtttgct aaatttttcg
     3181 acaacatgga tcctcttcct caccaccttc atttagatga tgaaaaagca gcacgagtag
     3241 ggcaaaaagg aaagccagaa gggtattatt ttccccgtca attgaacaac catggaggct
     3301 atttccctta tacttttttt ggtttgaatc caggaaccac tcgagatgat attcttcaat
     3361 gcctgaaaaa ttgggataaa ggggataatg gtattttata tctttcccga gcctacaaac
     3421 tccaattggg aaccggatgg aatgttcctc ctggagtttt acatgctcct gggtctttgc
     3481 ttacttatga gccacagaga gcttctgatg tttttgctat gtttcaaaat attgtctggg
     3541 atagatatct ttcctgggat atgttggtaa aagatgttcc tcctgaacat aaaaaagatt
     3601 tagactatat agttgacctc atagattggg aactaaacgt agaccccgat ttttataacc
     3661 agtattttct aaagcctaag ccggttaagg ccatagaaga aatggaaaaa gaaggctatg
     3721 aagaatactg gatagcatat aatactgaat atttttctgc taaagagctc accatatttc
     3781 cccgaaccaa ggtaaccata agagaagacg cggcttatgg cttaattgta attgagggaa
     3841 gaggtaaatt tggaatcctg gaagtggaga cccctactat gattcgcttt ggtcaaatga
     3901 ctaaagatga gctattcgta actcaagaag cagcagtaaa cggtataact atagtcaacg
     3961 aaagtgaaaa tgaaaacttg gttatactaa aacactttgg acctaaatcc tgaaatggaa
     4021 gaagttattt tagaatttga taaactaact aaaaaatttc ccggagtagt agctctagat
     4081 gaagttagct ttgacatttt taggggagaa gtgcatgctc tggtaggaga aaacggagcg
     4141 ggtaaatcaa ccttgattaa aattgtcacc ggggtctacc aaaaaacgtc aggagaggtt
     4201 ttttatcagg gtaagtccat aaatttccgc agccctcacg atgctttgca aaacggaata
     4261 gctgctatat atcaagaatt taatctcatt cctgccttga ctgtggcaga aaatatcttt
     4321 atgggtcacc attttgttcg aggaaaattt gttgattgga aaaaaatgga agaagaagct
     4381 cggaaactcc ttgacttttt ggaagtagac atagatgtta actcccgagt tcgagacctg
     4441 ggggtagcta aaaaacaagt aatagaaatt gctaaagctc tttctcataa tgctaaggtt
     4501 ctgattatgg atgagcctac cgctgcttta gctcaaaaag aaataaaaac tctctttcga
     4561 attatccgct ttctgaaaga aagcggggtt accatcattt atatctctca tcgcttagag
     4621 gaagtttacg aaatctgtga tcgggttacg gtgttacgag atggtaaatt tgtggctaca
     4681 aaaccagtga aagaattaaa agtggaagag cttatccgga tgatggtggg gagagaagtg
     4741 ggtgagagat ttccccgtgt ttcccatacc attggggaag aggttctgag agtggaagga
     4801 ttaactcgcc ccggagtctt ggaaaatata aatatttccc tccatcagag ggaaatatta
     4861 ggtattgcag gaatggtagg ctcaggaaga acagagctct tacgagctat atatggtgta
     4921 gatccctggg aggaaggaaa aatattcatc aggggagaag aagtaaaaat tacctctcct
     4981 cctgaagcaa ttagcttggg aattgcgctt cttcctgaag agagaaaaac acagggactt
     5041 attcttttac tttcggttct tgacaactta ggtttaccca ttctacctaa cattgctaat
     5101 gcaggattta ttgatgaaga tagattaaag caaattggtg aagaaatggt agaagctatg
     5161 aatattaaaa caccttctct ataccagaga gtcatgtatc tttctggagg gaaccagcaa
     5221 aaagtagttt taggtaaatg gtttgccagg aagtgtgata tctatctttt tgatgagcct
     5281 acccgaggaa tagacgtggg agctaaggta gaaatttacc aattgatgaa tcgtttagta
     5341 agtgaagggg cggccataat tatggtttcc tcagaactac cagaaatttt agctatgagt
     5401 gatcgaattt tagtaatgag agaaggaaag atagctggag aagtaaatag agaggaggcg
     5461 aatcaagaat tgattctaaa cttggcgctg ggaggaaaga atcattatgc ccgaacccct
     5521 tgaaacgaag aaagaaaaag ttagccttgt agaacggttt ttcattttat gggatagaat
     5581 aggtatttta ttggctttta tagtggtgtg tattttattt ggaattctaa cccccgtttt
     5641 cttcaatccc ctcaacattt taaatgttat ccgccaggtt tctataatcg gagtaatcgc
     5701 agtagggatg acttttgtaa ttctcctggg aggcattgat ttatcggtag gatcagtggt
     5761 ggcttttacc ggaattattg ctgctggttt ccaagtaaaa tggggaggaa gcctattttt
     5821 gagcatagtt attcctctcc taataggcgg aggtattggc tttctcaatg gttttatttc
     5881 tacaaaagga ggcctccatc ctttcatcgt taccttagga actatgagca tatttcgggg
     5941 tgccactctt ttagtggcta
//
LOCUS       DAOPSS010000095         5920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_10969_length_5920_cov_9.279113, whole genome shotgun sequence.
ACCESSION   DAOPSS010000095 DAOPSS010000000
VERSION     DAOPSS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10969_length_5920_cov_9.279113"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKN31_06215"
     CDS             complement(<1..210)
                     /locus_tag="PKN31_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99552.1"
                     /translation="MGKQEGCGGVSIVLSFPKFSRESTPHRHSFFVIPEIFYRESMFF
                     LFLFVLLSFCLLGVMRGINPPHSHDR"
     gene            488..955
                     /locus_tag="PKN31_06220"
     CDS             488..955
                     /locus_tag="PKN31_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOA99553.1"
                     /translation="MPRCRRGGPPWWEPRFQEAGFRLTMPRQAILEVLSNTEEHPSAE
                     EIYLAVQKIYPNIGLTTVYRTLELLVDMGLVFKFDFGDKRARYELSQGPTSKKHHHHL
                     VCTNCGRIIDYTDFIDEEKELLRRTEAGLSEKYNFKITDHLIQFYGLCDECRG"
     gene            1017..1385
                     /locus_tag="PKN31_06225"
     CDS             1017..1385
                     /locus_tag="PKN31_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOA99554.1"
                     /translation="MPGGDRTGPWGRGPMTGRATGYCAGYSVPGYMNPIPGRGWGAGF
                     GRGCGRGRGWRNWYRATGLPGWARAGYGYPAFGGWAYPYAPAPTPQAEAELLREQAEL
                     LKQALEDIQNRISALEKAEV"
     gene            1482..1859
                     /locus_tag="PKN31_06230"
     CDS             1482..1859
                     /locus_tag="PKN31_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOA99555.1"
                     /translation="MKIAISTEGNSVSPHFGRCPFFTIIDLEDGKVIKKEVIPNPGHE
                     PGFIPQFLHELGVECIIAGGAGKRALSLFQESGIQVILGVTGEIDEVIDKLKDGSLEG
                     GESLCKPGLSKGHTGKECNHHSE"
     gene            1880..2263
                     /locus_tag="PKN31_06235"
     CDS             1880..2263
                     /locus_tag="PKN31_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOA99556.1"
                     /translation="MKIGITARGNNLDSEVDPRFGRCEYFIIVDPDTLEFEVIENFSM
                     EAASGAGVQSGQLVATKGVEAVLTGNIGPNAFQVLQAAGIKVITGVSGKVEEAIEKYK
                     RGEFKPVESSNVDSKFGISEKGMGG"
     gene            2270..2473
                     /locus_tag="PKN31_06240"
     CDS             2270..2473
                     /locus_tag="PKN31_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99557.1"
                     /translation="MPQGRGFGQGAGQGQGRGLGRRGGRRPGAGADGNCVCPNCGTKV
                     PHQRGVPCYSLACPQCGAGMVRE"
     gene            2470..3324
                     /locus_tag="PKN31_06245"
     CDS             2470..3324
                     /locus_tag="PKN31_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA99558.1"
                     /translation="MIVSVASGKGGTGKTTVAVSLALSISEEEVQFLDCDVEEPNASI
                     FLKPQIEEALPVSIPVPRIDQEKCNFCGKCAEVCAYHALAVLPGKVLVFPHLCHGCGG
                     CSLLCPQGAITEVGREIGTMEIGRSGDIEFIQGRLNVGEAMPTPLIRAVREEAKKRRN
                     PAAVTVIDASPGTSCSVVEAVKGSDFCILVTEPTPFGLNDLILAVEVLRKLGIPFGVV
                     INRSGMGNDKVDFYCQENNIPILLRIPFDREIAVLYSQGIPIVQGKKEYMERFRGMMD
                     HIKELSRA"
     gene            3321..4247
                     /locus_tag="PKN31_06250"
     CDS             3321..4247
                     /locus_tag="PKN31_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA99559.1"
                     /translation="MKQILVISGKGGTGKTVLTSSLAALARGAVLADCDVDAANLHLV
                     LHPVVKEVQDFWSGQKAAIDYEQCINCGRCLSLCRFGAVKTLKNQDGSLKKLVIDPVA
                     CEGCRVCYYTCPVDAIRMEDRLSGQLFISETKYGPMVHAKLGIAEENSGKLVALVKQK
                     AQEIAEAEGKTWLIIDGPPGIGCSVIASLAGVDLALIVAEPTLSGIHDMERVMDLAVH
                     FGVLAKVFINKCDINWDNTRTIEEICQKRGIEVIGRLPFSEEVVRSLVAGVPVVKFSQ
                     GEIAEEITTLWRKIEAIFGEGVVLSGKKEQRF"
     gene            4222..5034
                     /locus_tag="PKN31_06255"
     CDS             4222..5034
                     /locus_tag="PKN31_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA99560.1"
                     /translation="MAKKNRGFRYIYGPVASWRLGASLGIDLISQKEKVCNFDCVYCQ
                     LGKTLVFPQQQKTYVPTEAIIAELKSLPEVQLNYITFSGRGEPTLAENLGQAIKAVKE
                     VRSEPVAVLTNSSLMSYPEVREDLSGADLVVAKLDAYSSDSLSKINRPQPGIEFDLVV
                     EGIRQFRANSNTRLALQIMFIEENKNQPEEFWPLLDYIQPDEVQINTPLRHSPIPPLS
                     PEDIFQIKKCFSAYNEHLPEEGKINLVSVYDAKPKEVFPISDESTLKRRGKV"
     gene            5073..5909
                     /locus_tag="PKN31_06260"
     CDS             5073..5909
                     /locus_tag="PKN31_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA99561.1"
                     /translation="MWWSFNKILVATTSGLSGFGEKIKAEQPALSLKERKEAEKPVEV
                     KITVIYDNYSLVEGLETEWGFSCLIEGLEKTILFDTGGRGDLLLENMKKLNINPREID
                     IVFISHDHWDHTGGLLSFLQENPQVEVWLLDSFSPNLKNEVTRSGAILREIKGPSPVC
                     SGAYSTGTLGDAIKEQSLVIESKEGLVLVTGCAHPGVLDIIRSVKSSSNQDIYLVMGG
                     FHLLDLSPEELQEIADRFKESGVKKVGPSHCSGDLTRELFAQEYKQDLVEVGVGKTIS
                     FP"
BASE COUNT         1734 a         1043 c         1548 g         1595 t
ORIGIN      
        1 cctgtcatga ctatgaggag gatttatccc cctcataacc cccaaaagac aaaaagacaa
       61 aaggacaaaa agaaaaagaa aaaacatgga ttcccgataa aagatttcgg gaatgacaaa
      121 aaaggaatga cggtgggggg tggattcccg gctaaatttc gggaatgaga gaacaataga
      181 aaccccccca caaccttcct gctttcccat tgctcttcaa gatagctttc tgaatcctct
      241 ttgtaattcc tgtgaaaacc ctccaagaca gcgcttttta tactcttata aatttccaga
      301 tgagcattat ggctttgggc agcaagtttc ctcgtttcat tccttatacc tctatgtaat
      361 ctctcctcta tttttactta agtgttaagg tatgattttt caaacggtct ctaagattat
      421 tgacaaactt caactgacca tttataatgc taatgaaaac cattttcaat aacaagaggt
      481 tttaagtatg ccaagatgta gaagaggagg gccaccctgg tgggagccca gatttcagga
      541 agccggtttt cggctaacta tgcccagaca ggcaatatta gaagtgctga gcaatactga
      601 agagcacccg agcgcagaag agatatattt agcagttcaa aaaatctacc ccaatatcgg
      661 gctcaccact gtttaccgga ctttagagtt actggtagat atgggattgg tgtttaaatt
      721 tgattttgga gacaaaagag caagatatga gttatctcaa gggcctacga gcaaaaaaca
      781 tcaccatcat ctggtatgca ccaattgtgg tcgaataata gattacactg actttattga
      841 cgaggaaaaa gaactgctcc ggcgaacaga ggcagggctt tccgaaaaat acaatttcaa
      901 aatcaccgac catcttattc agttttatgg tttatgtgat gaatgtcgag ggtaacttaa
      961 aaattttttg gtaataaatg aaaatgattt tcaatattga aagggaggtg tttcaaatgc
     1021 caggtggcga tagaactggt ccttggggac gaggtccaat gactggaagg gctactggtt
     1081 actgtgcagg ttattcagtt cccggctata tgaaccctat tcctggtcga ggatggggag
     1141 caggctttgg ccgagggtgc ggtcgaggcc ggggctggag gaattggtac cgggctactg
     1201 gtcttcccgg ttgggcgaga gctggttatg gttatcccgc ttttgggggc tgggcttatc
     1261 cttatgctcc agcaccaact cctcaggctg aagcagagct actgagagaa caggctgagt
     1321 tattgaaaca ggccttagaa gatattcaaa atcgaattag cgctctggaa aaagccgaag
     1381 tataaaacaa gaagaataaa caaaagaaag gaggtaagaa tctttggtag cttttgtagc
     1441 ttttcttacc tcctttctca ccaaaaagga gtgataatat tatgaagata gctatctcta
     1501 ctgaaggaaa ttcagtctct ccccatttcg ggaggtgtcc gttttttacc attatagatt
     1561 tagaagatgg gaaggtaatc aaaaaagaag tgataccaaa tccggggcac gagcctggtt
     1621 ttattcccca atttttgcac gagttagggg tagagtgcat aatcgctgga ggtgcaggaa
     1681 aaagagcact tagtcttttc caggaatctg gtatccaggt tattttaggg gtaaccggtg
     1741 agatagacga agtaatagat aagctaaaag atggcagttt ggaaggtgga gagagtttat
     1801 gcaaacccgg tttgagtaaa ggccataccg gaaaagagtg taaccaccac tcggagtaaa
     1861 gaccagaaag gagttaaaga tgaaaattgg tataacggcc agaggaaata atttagattc
     1921 ggaagttgac ccccggtttg gaaggtgtga gtattttatt atagtagacc cggacaccct
     1981 ggaattcgaa gttattgaaa atttcagtat ggaggctgca agcggagcgg gggttcagtc
     2041 tggtcaattg gtagcgacaa aaggagtgga ggcagttttg accggcaata ttgggcctaa
     2101 tgcttttcag gtgttacagg cagcagggat aaaagtgata accggagttt ctggtaaagt
     2161 ggaagaagct atagagaaat ataaaagagg ggaattcaaa ccggtggaga gctcaaacgt
     2221 tgactccaaa tttgggatat cggaaaaagg aatgggggga tgagctatag tgccacaggg
     2281 aagaggtttt ggtcaaggag ctggccaagg acagggcaga ggattaggaa gaaggggagg
     2341 taggcgtccg ggagcagggg cggatggaaa ttgtgtttgc ccgaactgtg ggactaaagt
     2401 tcctcatcaa agaggagtgc cttgttattc tctggcttgt cctcaatgtg gggcagggat
     2461 ggttagagaa tgatagtatc ggtagccagt ggaaaaggtg ggacgggaaa aaccactgta
     2521 gcagttagtc tggctctttc tatctcggaa gaagaagttc aatttctgga ttgtgatgta
     2581 gaagaaccta acgcgagcat ttttttgaag cctcagatag aggaagccct accggtttct
     2641 attccagttc ccaggataga ccaggaaaag tgtaattttt gtggcaagtg tgctgaggtt
     2701 tgtgcctatc acgctttagc agtgcttccc ggaaaagtac tggtttttcc tcacctctgc
     2761 catggttgtg gtggttgtag cttgttgtgt cctcagggag caattaccga agtgggccga
     2821 gaaataggaa cgatggagat tggccgctca ggcgatatag agtttatcca aggtaggcta
     2881 aacgtgggag aagcgatgcc cacccccctc attagggcag tcagggaaga agctaaaaaa
     2941 agaaggaacc ctgctgctgt caccgtaatt gatgcctctc ctggaacctc ttgttcagtg
     3001 gtggaagcgg tgaaggggag tgatttttgt attttagtca ctgaacctac tccttttggc
     3061 ttaaatgact tgattctggc agtagaagtg ctgagaaagc tgggaatccc ctttggagtg
     3121 gtgataaatc gctcaggaat ggggaatgat aaagtcgatt tttattgcca ggaaaataac
     3181 attcctattt tattacggat accatttgat agggaaattg ctgttttata ctctcagggt
     3241 atacctatag tgcagggaaa aaaggaatat atggaaaggt ttcgagggat gatggaccat
     3301 attaaggagt tatcgcgggc atgaaacaaa ttctggtaat tagcggaaaa ggtggaacag
     3361 gaaaaactgt tttaacttca tctttggcgg ctttggccag aggagcagtt ctggctgatt
     3421 gtgatgtgga tgcagctaac ctgcaccttg tattgcaccc ggtggtaaag gaggttcaag
     3481 acttttggag tgggcagaaa gcggcaattg attatgagca gtgcatcaat tgtggtcgtt
     3541 gtctctctct ttgtagattt ggagccgtta agactctaaa aaatcaagat ggtagcttaa
     3601 aaaaattggt gattgaccct gttgcttgtg aaggatgtcg agtttgctat tatacctgcc
     3661 cggtggatgc tatccggatg gaagacagac tttcggggca gctatttatt tctgagacta
     3721 agtatggtcc aatggtgcac gctaagctgg gaatagctga agagaactcg ggtaaattgg
     3781 ttgctctggt gaaacaaaag gcgcaggaaa tagccgaagc ggagggaaaa acctggctta
     3841 tcattgatgg tccacccggt ataggatgct cggtaatcgc ttctcttgcg ggagtagact
     3901 tagctttaat agtagctgaa cctactttgt caggtattca cgatatggaa agagtaatgg
     3961 atttggctgt tcactttggt gtgctcgcta aagtttttat aaataaatgt gatattaatt
     4021 gggataacac ccggacaatt gaggagattt gccaaaaaag gggaatagag gtgataggac
     4081 gcttaccttt ctcagaagaa gtagtgcggt ctttggttgc gggagtgccg gtggtaaaat
     4141 tttctcaagg agagatagcc gaagaaataa ctaccttgtg gaggaaaatt gaagctatct
     4201 ttggggaggg ggtagtcctg agtggcaaaa aagaacagag gttttagata tatctacggt
     4261 ccggtagctt cctggaggct gggagcttcc ttggggatag accttatttc tcagaaagag
     4321 aaggtatgca attttgattg tgtttattgc caattgggta aaactttggt ctttcctcaa
     4381 cagcagaaaa cctatgttcc cacagaggca ataatagcag agcttaaatc tctgccggag
     4441 gttcaattga attatattac tttttccggt cggggtgaac ctactttagc tgaaaatttg
     4501 ggccaggcaa ttaaggcagt aaaagaggtt cgttctgagc ccgtggcagt tttaaccaat
     4561 tcatctctga tgagctatcc tgaagttaga gaggatttat ccggggcaga tctggtagta
     4621 gccaagttag atgcttattc ttcagattct ctttcgaaaa tcaatcgccc tcagccgggg
     4681 atagagttcg acctcgtagt agaaggaata aggcaatttc gagcaaacag taatactcgg
     4741 ctggctctcc agattatgtt tatagaagag aacaaaaatc agccggaaga gttttggccc
     4801 ctcctcgatt atatccagcc ggatgaggtt caaattaaca cccccttgcg tcatagtccc
     4861 ataccgcctc tctcccccga ggatattttc cagataaaaa agtgtttttc tgcttataac
     4921 gagcatcttc ctgaggaggg gaaaataaat ctggtttcgg tttatgatgc taaaccaaaa
     4981 gaggtcttcc ctattagtga tgagagtacc ctcaaaagaa gaggaaaggt gtaaaatcac
     5041 ttaaacaatt taccgaaaga gaggcggtct ttgtgtggtg gagctttaat aagattctgg
     5101 tagcaacaac ttcagggctg tcggggttcg gagaaaaaat aaaagcagaa caacctgctt
     5161 tatccctcaa ggagagaaaa gaggcggaaa agccagtaga agtaaaaatc actgttatct
     5221 atgataacta ttcgttagtt gagggtcttg aaacggagtg gggtttttct tgcctgattg
     5281 aaggtctgga gaaaactatt ctttttgata ccggtggaag aggcgacctg ttactggaaa
     5341 atatgaaaaa gctaaatatt aatccccggg aaatagatat agtttttatc tctcatgacc
     5401 actgggacca caccggtggc ttattatctt ttcttcaaga aaatcctcag gtagaagtct
     5461 ggttgttaga ctccttttca ccgaatctga aaaatgaggt cacaagaagt ggggctatct
     5521 taagggaaat taaaggacca agcccggttt gctcgggagc gtattctact ggaactttag
     5581 gggatgcaat caaagagcaa tcactggtta ttgaaagtaa agaagggtta gtactggtaa
     5641 ccggttgtgc tcatccgggg gtgttagata tcattcggtc ggttaaatct tcctctaacc
     5701 aggatatcta tctggttatg ggcggttttc acctgcttga tctatctccg gaggaattac
     5761 aagaaattgc tgatagattt aaggaatcag gagtgaaaaa ggtagggcca tctcactgta
     5821 gtggtgacct cactcgggag ttgttcgctc aagaatataa gcaggatctg gtagaagttg
     5881 gagtgggtaa aaccattagt tttccctgaa gtcaaagcct
//
LOCUS       DAOPSS010000096         5672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_11584_length_5672_cov_8.899769, whole genome shotgun sequence.
ACCESSION   DAOPSS010000096 DAOPSS010000000
VERSION     DAOPSS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11584_length_5672_cov_8.899769"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PKN31_06265"
     CDS             <1..917
                     /locus_tag="PKN31_06265"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOA99562.1"
                     /translation="EMKGELLVAHVPKTIDNDLPLPGNMPTFGFQTAREVATQIVKNL
                     MEDAKTTGRWYIVVMMGRSAGHLALGVGKSAGAHLVIIPEEFGERTVKVEEVCDIIES
                     SMIKRKALGRSDGIAIVAEGVALKFGDVEEIEKMLGRPVPRDPHGHVRLAEVPLGTLL
                     KDEIARRFEARGEKITMVTGDVGYELRCAPPVAFDMEYTRELGYGAVNYLLSPDYSLD
                     MKKKGAMISILEGVLNPIPFPEIMDPETGRTKVRTVDINSYSYEVARSYMIRLEKKDL
                     EDEEMLTGLAQAANLSKEEFKARFSKVV"
     gene            950..3178
                     /locus_tag="PKN31_06270"
     CDS             950..3178
                     /locus_tag="PKN31_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOA99563.1"
                     /translation="MTSFFRYPAFWVVVAALAGFFLQSYFSFSLLWLFLFLFLSLFLA
                     VLFFSRSRIFFWSIIVVWLAVGATVHALSFDLPREMVLREEGKTITIQGNLIEKNNRY
                     FITRTSNLSFYSPSLLLRGGDFKPEEWGYRVRIRAVVRSFNSCSNPGVMDFRLFWGRK
                     RVPAYLEVESWEVVSPPRGWFSLMHQVEEKKRGFLSSWKEELQDEYPWFSALLVGVKE
                     EEFEERRDLLQEVGIYHAFCVSGLHLALLGGIIWFFLNRITFIKRFSWIGALFFTFWY
                     LLFCSISPSSFRAWLFFSLGLWGKIKGRHTLPVSSLLTAFLIMFLLQPEVVYDAGAQL
                     SFASTAGILFLMDYGENFRNKKHFLLGYLGRGVFLTFSVYVFNLPFLIFRGFSFSSLS
                     LLGNLLVLPLLEAALFLSFLSAFLGGIVFLRPLLAFLLRSALRASLFVSAFLEEHVPH
                     FFWDFSQIRNQLWGWVFWLSLLGFFIFLIRRERKKLIWGSVLVGVLLVGGLYLFLPPP
                     LEFWVFDVGQGLATGIVEERTMSFIDAGGIIRGYGNTGKAVLEKFIHYRDIEKVDYVF
                     LTHFHQDHSAGIEPLSSSFPELAVLSLPYFPNFSCFPLSSRAQLEIFPIQGKKENEQA
                     LVFRLTLPHLRILICGDVEEEGIRYLMGWGEENLKAEVVILPHHGKYVSNMAELLSLC
                     DCEVAVISCGENSYGHPSPETIKLLADLNLPYFITQEDGAICFRPTGKSWEVKRFGKG
                     RI"
     gene            3159..4091
                     /locus_tag="PKN31_06275"
     CDS             3159..4091
                     /locus_tag="PKN31_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99564.1"
                     /translation="MGKVEFKTWMNQDIKGRCFVLELAVGGKYFEDHCWPQIVEKLGG
                     GKVVRIKGKKEWEEKGGGAILPSLFGEKKILLLEDLEESLALKVKNISSPHFLVFISS
                     GWRGGKEWSDDTWIKVEPPRYDLENFWKKSAKNKGIFLDNQVLRSLSRWSKEYDWGEE
                     DVEQFLSQFSEGEIVKMEDVELYFEKNERTLLFRFLDATGERNLEGAIFYLRSLLQIG
                     FSPSLLLTNVARRFRLLWQAQETKEETVDLWSGRKLSPFEWREKILKNKNKYNLEEIQ
                     RAFEILYQVDKALKTRNPEPEALLLGMLVKIMAK"
     gene            complement(4084..4371)
                     /gene="rpsT"
                     /locus_tag="PKN31_06280"
     CDS             complement(4084..4371)
                     /gene="rpsT"
                     /locus_tag="PKN31_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOA99565.1"
                     /translation="MPNTKTAWKNLRKNEKRRMRNRSIKSATKTEIKKFRKLVEEGMI
                     EEAHSYLPRVYKKVDMAVSKGVFHKNKAARIKSRLTNKLNQAAAQHESSLT"
     gene            complement(4545..5297)
                     /locus_tag="PKN31_06285"
     CDS             complement(4545..5297)
                     /locus_tag="PKN31_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOA99566.1"
                     /translation="MKKPIVFLSVLVLLVVLAGCGGQSSAPSPSQTQKPQLITVNDGS
                     KNLQNGDPVRGGVTVTFTGSGPADAIIRVYLNGTLLDSTTTNSAGSFTWSWTSGTTEG
                     TFAFAFTAKAEELLESDQETFSLVVDTTPPYFLSCSAKADAPLDAAPTITVQFNEPVV
                     IGDVNLFEHLAYWAVSCVACPGGSHFTVTEATLEEDSQTVTLTGKWDTDQLVTGDQVM
                     VHFIYNSLMDIKDRAGNAFSTTVSILTCTVTP"
BASE COUNT         1440 a         1125 c         1390 g         1717 t
ORIGIN      
        1 gggagatgaa aggagagcta ctggtggctc atgttcccaa aaccattgat aacgaccttc
       61 ctcttcctgg taacatgccc acttttggtt tccagacagc acgggaagtg gctacccaga
      121 tagtgaaaaa tctgatggaa gacgccaaaa ccaccggacg ctggtacata gtggtgatga
      181 tgggacgctc tgctggacat ctcgctttag gagtgggaaa gtctgctgga gcgcatcttg
      241 ttataatccc cgaggagttt ggcgagagaa cagtaaaagt agaagaagtg tgtgacatta
      301 tcgagtcttc catgattaag cgaaaagccc tgggaagaag cgatggaata gcaattgttg
      361 ccgaaggagt ggctttgaag tttggcgacg tagaagagat agaaaaaatg ctgggaagac
      421 ctgtccctcg tgacccccat ggtcatgtgc gtctggccga agtgcctctg ggaacactat
      481 taaaggatga aattgctcgc cgctttgaag ctcgaggaga aaaaatcacc atggtaaccg
      541 gagacgtggg gtatgagctg aggtgtgcgc cacctgtggc ctttgatatg gaatacacgc
      601 gggagttggg gtatggagca gtgaactatc ttttaagtcc cgactatagt ctggatatga
      661 agaaaaaggg agccatgatt agcattttag aaggcgtgct aaaccccatt cctttcccgg
      721 aaataatgga cccggagacg ggaagaacta aagtacggac tgtggacatc aattcgtact
      781 cttacgaggt agctcgttct tatatgattc gtctggagaa aaaagattta gaagacgaag
      841 agatgcttac tgggttagca caggctgcta acttaagtaa agaagaattt aaggctcgtt
      901 ttagtaaagt agtataggct gccggggccg cgcgagcggc cccctttcta tgacttcctt
      961 ttttcgttat cctgccttct gggtagtagt tgctgcactg gcgggtttct ttttgcagag
     1021 ctatttttct ttttctctcc tctggctgtt tttattcctt ttcctttccc tttttcttgc
     1081 agttttattt ttctctcgga gccgaatttt tttctggagc ataatagtgg tgtggctggc
     1141 ggtgggcgct acagtgcatg ctttgagttt tgacctacct cgagaaatgg tcctgagaga
     1201 agaaggcaag accattacca ttcaaggaaa tctgatagaa aaaaacaatc gttatttcat
     1261 tacccggacc tctaacctct ctttttattc tccttcttta cttctccggg ggggagattt
     1321 taagcctgaa gagtggggat atagagttcg tatccgtgct gtggtgcgca gcttcaattc
     1381 ttgctctaac cccggggtaa tggattttcg ccttttctgg ggaaggaaaa gagttcctgc
     1441 ttatcttgag gtggaaagct gggaggtggt ctctcctcct cgagggtggt tttctctgat
     1501 gcaccaggtg gaggaaaaga agagggggtt tctttcttca tggaaggaag aactacagga
     1561 tgaatatccc tggttttctg ctcttcttgt gggggtgaaa gaagaagagt ttgaggaaag
     1621 aagggatttg ctccaggaag tgggcatcta tcatgctttt tgtgtatcgg ggttgcacct
     1681 tgctcttctg gggggaataa tctggttttt cttgaaccgg ataactttca taaagcgatt
     1741 ctcctggata ggggctttat tttttacctt ctggtatttg cttttttgta gcatttctcc
     1801 ctcttctttt cgagcctggc tcttttttag cctgggtctc tggggaaaaa taaaaggtcg
     1861 ccacactctt cctgtctctt ctttgttgac tgcctttctt atcatgtttt tgcttcagcc
     1921 tgaggtggtc tatgatgcgg gggcgcagct gtcttttgct tccactgccg ggattctttt
     1981 tttgatggat tatggagaaa acttccggaa taaaaagcac tttctccttg gctatctcgg
     2041 gagaggggtt tttctcactt tcagtgttta tgtttttaac cttccctttt tgattttccg
     2101 gggtttttcg ttttcttctc tttctctttt gggaaatttg ctggttttac ctctccttga
     2161 agctgctctt tttctctctt ttttgagtgc ttttctgggt ggaatcgtct ttcttcgccc
     2221 tcttcttgcc tttcttttga gaagcgctct tcgggcttct ctttttgttt ctgcattttt
     2281 agaagagcat gttcctcatt ttttctggga tttttctcag ataagaaatc agctgtgggg
     2341 atgggttttc tggctctctc ttctcggatt ttttattttt ctcatcagac gagagaggaa
     2401 aaagttaata tggggttctg ttttagtcgg tgttctttta gtggggggac tttatctttt
     2461 ccttccccca cctcttgagt tctgggtttt tgacgtagga cagggtctgg caacggggat
     2521 agtagaggaa aggacaatga gctttatcga tgccggagga ataataaggg gctatggtaa
     2581 tacaggaaaa gcagttttag aaaaatttat ccactatcgg gatatagaga aagttgacta
     2641 tgtttttctc acccattttc accaagacca ctctgcaggt atagagccac tatcttcctc
     2701 ttttcccgag cttgcagtat taagtcttcc ttattttccc aatttttctt gttttcctct
     2761 ttcttctcgt gctcagttag aaatctttcc catccagggc aagaaagaaa acgagcaggc
     2821 tctggttttt cggttgactt tacctcattt acggatttta atttgtggag atgtggaaga
     2881 agaaggaata agatatctta tggggtgggg agaggaaaac ttgaaagccg aggtggtgat
     2941 acttccccat cacggtaaat atgtcagcaa catggcagag cttctctccc tttgtgattg
     3001 tgaggtggca gtaatttctt gtggagaaaa tagctacggg caccccagcc ccgagaccat
     3061 aaagttgctt gccgacttaa acttgcctta ttttataact caagaagatg gggcgatttg
     3121 ttttcgcccc acaggaaaaa gttgggaggt taaaagattt gggaaaggta gaatttaaaa
     3181 cctggatgaa ccaggatata aaaggtagat gttttgttct ggagctcgcg gtgggaggaa
     3241 aatattttga agaccactgc tggccacaaa ttgtggaaaa gctgggaggg ggaaaagtgg
     3301 tgaggattaa agggaaaaag gagtgggaag aaaaaggggg aggggcgatt ttaccttctc
     3361 ttttcgggga gaagaagatt ctgttgctgg aggatttgga agaaagtctc gctctgaaag
     3421 taaaaaacat cagctctccc cattttctgg tatttatctc ttcaggctgg aggggaggaa
     3481 aggagtggag tgatgacacc tggataaaag tagagcctcc tcgctatgat ctggaaaact
     3541 tttggaaaaa gagcgcaaag aacaagggaa tattcttaga caatcaagtt ctccggtctc
     3601 tttctcgctg gagcaaggaa tacgattggg gagaggaaga tgtagaacag tttttgagcc
     3661 agttttcaga aggggaaatc gtaaaaatgg aagacgtaga gctttacttt gagaaaaacg
     3721 agcgaacgct cctttttcgc tttctggacg ccacaggaga gagaaacctg gaaggagcta
     3781 ttttctacct tcgctctcta ctgcagattg gtttttctcc ctctttgctt ttaaccaacg
     3841 ttgctcgtcg ctttcgtctc ctgtggcaag ctcaggagac gaaagaagaa actgtagacc
     3901 tatggtcagg cagaaaactc agcccttttg agtggagaga aaaaattcta aaaaacaaga
     3961 ataaatataa cttagaagaa atacaaagag cttttgaaat tctctaccag gtagataaag
     4021 cactaaaaac ccggaatccc gagccagaag ctttacttct ggggatgttg gtaaaaataa
     4081 tggctaagta agagagcttt cgtgctgagc agcagcttga ttaagcttgt tggtgagacg
     4141 agatttaatc cgagcagctt tatttttatg aaaaacccct ttgctcactg ccatatcaac
     4201 tttcttatac acccgaggaa gataagaatg agcctcttct atcattcctt cttctacgag
     4261 ctttctgaac ttcttgattt cagttttagt agctgatttg atgcttcggt tacgcattct
     4321 tctcttttcg ttttttcgca aatttttcca ggccgtttta gtgttgggca ctttttccac
     4381 ctcttaaaag attttttaaa aaaagacgat gctaatttta acacggagct ttaatatttg
     4441 caaccggaag gagctcagga tagtgtaaat ttatgaggaa aactttcagg ctctccttaa
     4501 agaccacagc ctgcccgggc aggctgtggt gcaggttcga aatcctatgg agtaaccgta
     4561 caggtgagaa tgcttactgt agtactgaaa gcattcccgg ctcggtcctt aatatccata
     4621 agggaattgt aaataaagtg gaccatcacc tggtcaccgg taaccagctg gtctgtatcc
     4681 cattttccgg ttaaggtaac cgtctggctg tcttcttcaa gggttgcttc agtcaccgtg
     4741 aaatgagagc cacctgggca tgcaacacag ctcacagccc aatatgcaag atgttcgaag
     4801 agattcacgt ctcctataac taccggttca ttaaactgaa cagttatggt gggcgctgca
     4861 tcaagggggg cgtctgcctt tgctgagcag gaaagaaaat aaggtggtgt tgtgtctacc
     4921 acaagagaaa aagtttcctg atcgctttcc agaagctctt cggccttagc ggtaaaagca
     4981 aaggcaaagg tcccttcagt ggttccactg gtccagctcc aggtgaagct tcctgcagaa
     5041 ttggtagtgg tactatcaag aagagtgcca ttcagatata ccctgataat agcatcagcc
     5101 ggcccacttc cggtaaaggt aactgtaaca ccacccctca ccggatcacc attttgcaga
     5161 ttctttgagc catcatttac agttatgagt tgaggctttt gagtttggct gggcgaggga
     5221 gcagagctct gaccaccaca ccctgcaagc accacaagaa gcaccaacac agagagaaaa
     5281 actattggtt ttttcatctt ttttcctcct cttctcctaa ttcaaaatac tattttaaag
     5341 ctcgtcagag aataaatcaa atctttgcac ttgtcagtgt ctatgaaagt ggttggctac
     5401 gctaaatatt gttttacggt tgaaaaaagt agtaagaata gagagctttt tgctcccctg
     5461 ctaaatcggg gttgtatctt ggatatatta gatgtcgagg actctcgtga cgaaaagcag
     5521 ggctttgcgg ttttctggct cattccccgg ctcacccaat atctcttctg cgtgccggga
     5581 ttctcttcga gctctaaagc aggtgtctta atgaaccaaa aagtgcccaa ttgcgattga
     5641 taaaaaatgt caattattgt atgatggtag aa
//
LOCUS       DAOPSS010000097         5651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_11637_length_5651_cov_8.027162, whole genome shotgun sequence.
ACCESSION   DAOPSS010000097 DAOPSS010000000
VERSION     DAOPSS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11637_length_5651_cov_8.027162"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..2693
                     /locus_tag="PKN31_06290"
     CDS             270..2693
                     /locus_tag="PKN31_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOA99567.1"
                     /translation="MRYGHFEGREYVITNPHTPVKWINYVGTIDFGGFVDHTGGMLIC
                     KGDPALNRITKYITQLPASEFKGSTIYVRRRKANRNFLYSPYYVPTLHLYDLYECRVG
                     LGYSRFIMECEGLRSEITVFVPEGESVVLQDVRIKNIGKDPIDELDIIPVVEYSHFDA
                     LKQLTNADWVPQTMTSKAIGNPGSLLILRQCAFMQIGRAENFLTSNWPVSSFETDRKR
                     FLGANEYGTWTSPLALKQKELSNFEPLRGDNIGALIHHLGTLQPGEERRLIIQLGQAE
                     SVEKAIPAIERFRKPEEVDAALSQMEYFWENYLNVCQVETPDREFNLLVNTHNPRQCF
                     ITLNWSRYLSYYQLGYGERGIGMRDSSQDVMAVVSGAPERAKNLLRKLLSIQRQNGSA
                     MHQFNPQTMEATIGDAQEMKDRPKYYGDDHLWLVFAVCHYLTETGDYTFLEEKIPFYE
                     KGEDGKPLEVVTVLEHLERAVEFTWNNVGAHGIPLLGFADWNDTINLRNGAESFFIAH
                     QFSKACRDLCELMEYLGEEEKVKKHQAYAEEMKKRVNEVGWDGEWYLRYFDYDGTPLG
                     SKQNTQGKIFTNAQSWAVISGNAPLERANKALDAVYTYLHTEYGIKLSTPGYDHYDPN
                     IGGVTTYPPGAKENGGIFLHANPWVIIAECMMRNGERAYQYFSEINPIKKNEIIDIYE
                     VEPYVFCQNILGDEHPQFGLGRNSWLTGTASWMYQAATQYILGVRVSHQGLIIDPCIP
                     FEWKIYTVRKKTRGAWYTITVHNPSHVSEGVRSCTVNNETVPLDNGRVVIPFAPEGSE
                     NTVEVVLGQ"
     gene            2975..3640
                     /locus_tag="PKN31_06295"
     CDS             2975..3640
                     /locus_tag="PKN31_06295"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HOA99568.1"
                     /translation="MSSPERKFAVLTADIIGSRKLKDRGFSREQIEQAISELNIARKT
                     FIASKFCLLRGDEIQGVVFPPQKAIMMVRYLRFYLRPVQVRVGIGIGDITTEIDRNNP
                     WNMDGSSFHRARKALEEIKISRQPATFINSLEENFDYNLNVILALMDVISNRWTDGQW
                     EAIHTYEQMGTYQKAAQVLKVRPQNVAKRCQAAHWKVFSEAEKHIHNLISSKFSADNE
                     EEN"
     gene            3641..4393
                     /locus_tag="PKN31_06300"
     CDS             3641..4393
                     /locus_tag="PKN31_06300"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HOA99569.1"
                     /translation="MELSSKWLILELLLFSHGVADFVLQSNKMIENKVTRRRTTYISH
                     FFIVWGLSVLFLYPFISWQAGGVLLGLAFTHLVIDWIGYHLTPPHKIDSNPLGFVLDQ
                     LSHLIFILLAWQWLCHFSHLSSSGFYSEIMTPARIEKLTSVMVVGNVYLYVVFGGAIF
                     IGKAMRSLPQEVRKYEITGMGRYIGILERTIILTFILYQAVTGIAFVLMAKSLTRYQE
                     LNNKSFAEYYLIGTLWSTALALIGGLFFYLCK"
     gene            complement(4965..>5651)
                     /locus_tag="PKN31_06305"
     CDS             complement(4965..>5651)
                     /locus_tag="PKN31_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOA99570.1"
                     /translation="NTTIPVSKSQIFTTAADNQTTVEVHVLQGERPMARDNVSLGRFQ
                     LTGIPPAPRGVPQIEVKFDIDADGILHVSAKDLATNKEQTITIKASSGLSKEDIDRMI
                     REAELHAEEDRRRQEEVEVRNQADNLIYTVEKNLRESGDKLSSETRAQVENALNALRE
                     AMNKDDIADIRRKMDDLQNASHAMAQELYQKTAQSEASASSQAGSSNETTQDENVVDA
                     DYRVVDDDNK"
BASE COUNT         1689 a         1137 c         1271 g         1554 t
ORIGIN      
        1 aaatttagag atcatggttg gcgggttgta tcaattatta tatactggaa aggcacagat
       61 acagtagcta tcaagcagaa gagaatctcg ccacagatga acggggattc aatattttag
      121 atacctctcc agattgagtg agcctttagc tataccaagc aactcggcat catctactac
      181 gattaccctg gtagaaagag aacctcaaag gagagccaac gctaccagga gttcgtcact
      241 ggtaaaaaat attttaggga ggtgtagcca tgcgatacgg ccactttgaa ggaagggaat
      301 atgtgattac taaccctcac actcctgtaa agtggattaa ttatgtaggg actattgact
      361 ttggtggttt tgtggatcac accgggggaa tgctcatctg caaaggcgat cctgcactta
      421 accggatcac taaatatatc actcaacttc ctgcctcgga gtttaaggga tccactattt
      481 atgttagaag aaggaaagct aatcgaaatt tcctctattc tccctattat gtgcccactt
      541 tacatttata cgatctttac gagtgtcgag tagggctggg atactctcgt tttattatgg
      601 aatgtgaagg gctccgctca gaaataacgg tgtttgtgcc ggagggggaa agcgtagtgt
      661 tacaagatgt aaggatcaaa aacataggaa aagaccccat agatgaatta gatataatac
      721 ctgtagtgga atactcccat tttgatgcac tcaaacagct tactaatgca gattgggttc
      781 cccaaactat gacaagtaaa gccataggaa acccgggaag cttacttatt ctcagacaat
      841 gtgccttcat gcaaataggg agagcagaaa atttccttac ttctaactgg ccagtttctt
      901 cttttgagac tgatagaaag cgcttcctgg gagctaatga atatggaacg tggacatctc
      961 cacttgctct caaacaaaaa gaattgagta atttcgaacc gctacgggga gataacattg
     1021 gagctttaat acaccacctt ggcactctcc agcccggaga agaacgaaga ctcattatcc
     1081 agctgggaca agcagagtct gtagaaaaag cgataccggc cattgagcgc tttcgaaaac
     1141 cagaagaagt ggatgctgct ctttcccaga tggagtattt ctgggaaaat tacctgaacg
     1201 tatgccaggt ggaaactccg gatagagagt ttaatctact ggtcaatacc cacaatcccc
     1261 gacagtgttt tattaccctt aattggtcca gatatctttc gtactaccag ttaggatacg
     1321 gagagcgagg cattggcatg agagattcca gtcaggacgt aatggctgta gtctctggag
     1381 ctcccgagcg ggctaagaat ctactcagaa aactcctttc aattcaaaga caaaatggtt
     1441 cagcgatgca ccagttcaac ccccaaacta tggaggctac cataggcgat gcacaagaaa
     1501 tgaaagatag gcccaaatat tatggagatg accatctgtg gttagttttt gccgtatgtc
     1561 attacctcac agagactggt gattacactt tcttagagga gaaaataccc ttttacgaga
     1621 agggcgaaga tggcaaaccc ttagaggtag ttactgttct ggaacacctg gagcgagctg
     1681 tagagtttac atggaacaat gtaggtgctc acggaatacc ccttctgggc tttgcagact
     1741 ggaacgacac aataaatctc agaaacggtg cggagtcatt tttcattgcc catcagttca
     1801 gtaaggcatg cagagatctc tgtgagctta tggagtattt gggagaagaa gaaaaagtta
     1861 agaaacacca ggcctacgct gaggaaatga aaaagcgagt aaatgaagta ggatgggatg
     1921 gtgagtggta tttacggtat tttgactatg atggtactcc tctgggttcc aagcaaaata
     1981 ctcagggaaa gatttttacc aatgcccagt catgggcagt aatttcgggt aatgctcctc
     2041 tggaaagggc caataaagct ctagatgcgg tatacaccta tcttcatacc gagtacggaa
     2101 taaagctcag cacaccagga tatgaccatt acgatccgaa tatcggtgga gttactactt
     2161 atccccctgg tgcaaaagag aacggaggaa tcttccttca tgccaatccg tgggtaataa
     2221 tagctgaatg tatgatgaga aatggagaga gagcttatca gtacttcagc gaaatcaatc
     2281 ccatcaagaa aaacgagatc atcgatattt atgaagtaga gccctatgta ttctgtcaaa
     2341 atatcctggg tgacgagcac cctcagtttg ggttgggtag aaatagctgg ctcactggaa
     2401 cagcgagctg gatgtatcag gcagctaccc aatatatcct gggggtgaga gtttcgcatc
     2461 agggtttaat aatcgatcct tgtattcctt ttgagtggaa gatctacacc gtaaggaaaa
     2521 agacaagagg agcgtggtac acgattaccg tacacaatcc gtctcacgta tcagaaggtg
     2581 ttaggtcctg tacagtaaat aacgagactg tgcctctgga taatggccgg gtggtgattc
     2641 ctttcgcgcc agagggctcg gaaaatacgg tggaagtagt gcttggacaa tgagaaatga
     2701 gaagaaaata gtggaactca ctaactggtg agcaacctgc ggcacaagat aaaagtacaa
     2761 ataattaaga aattaaaata attcgtcgga aagatgcaga acaacatacg gtgtggaata
     2821 aaagatgtac tgagagagta aaaacttctc gatcatagaa ggataggctc ttttttcctt
     2881 attgttctgt gatataatca agcaagtgaa attctcacct tccaaaaggt gaaataaaaa
     2941 tataaccctt agtcgggtga aaaggaatca gtaagtgtct tctccagaga ggaaatttgc
     3001 tgtcttaact gccgatataa ttggctctcg aaaattaaaa gaccggggtt tttccagaga
     3061 gcaaatagaa caggccattt ccgaacttaa tatagccaga aagacgttta ttgcctctaa
     3121 attttgcctc ctgagaggag atgaaattca gggagtggtt tttccccctc agaaggccat
     3181 aatgatggtt agatacttac gtttttatct aagaccagtt caggtcaggg tgggaatagg
     3241 aataggagat atcactacag aaatagatag aaataacccc tggaatatgg atggcagttc
     3301 ttttcatcga gccagaaaag cactggagga aataaaaatt tctcgtcaac ccgctacctt
     3361 cattaattct ctcgaagaaa acttcgatta taatctgaac gttattctgg ctttaatgga
     3421 tgtaataagt aaccgctgga cagatggtca gtgggaagct atccatactt atgaacaaat
     3481 ggggacttat caaaaagcag cgcaggttct gaaagtgaga ccgcaaaatg tagccaaacg
     3541 ttgtcaggct gctcattgga aagtattttc tgaggcggaa aagcacattc ataacctgat
     3601 aagctcaaaa ttttccgctg ataatgagga ggaaaattga atggagctct ccagtaagtg
     3661 gctaatactg gagctattac ttttttccca tggtgtggct gatttcgttt tgcagagcaa
     3721 caaaatgata gagaacaaag taacaagacg acggaccacc tatatttctc acttctttat
     3781 tgtctgggga ctttcagtcc tgtttttata cccttttatt tcctggcaag cgggaggagt
     3841 tctcctgggc cttgctttta cccacttagt gatagactgg attggctacc accttactcc
     3901 tccccacaaa atagatagca atcctctggg ctttgtatta gaccagctat ctcacttaat
     3961 ttttattctc ctcgcctggc aatggctatg ccacttctcc cacctatctt cctctggttt
     4021 ttactctgag ataatgacac cagcccggat agaaaaacta accagtgtaa tggtggtagg
     4081 taatgtatat ctctatgtgg tctttggtgg agctatattt ataggaaaag caatgcgttc
     4141 tctaccccag gaagttagaa aatatgaaat aaccgggatg ggaagatata taggcatttt
     4201 agaaagaacc atcatcttga cttttattct ctatcaggca gttaccggga tagcttttgt
     4261 cttgatggct aagtcattaa ctcgatatca ggaattgaat aacaaatcat ttgccgaata
     4321 ttacctcata ggaactctct ggagcactgc tttagccctg ataggggggc tatttttcta
     4381 cctctgtaaa tgagctctag ggagcaatct acaaaaattt cagaaccact tttctactta
     4441 gcatattcaa atctgatata tttgctccat tgttagcatt cctttcttta cccctttaga
     4501 tttcacacct gaaagggtaa tgtattttta agggtgctgg caatggagtt tctatttcct
     4561 gctgtacttt ttaacttccg ctgtgctgca tttctattct accaaataca caaacatacc
     4621 tggtcgtttg gagctggtag ctgccatggc agtcgtgcaa gaaaatgtct tgtctataca
     4681 tgggggcatt ctgacagcaa gattggcggg gataaactgc gcgaaagatg gtagatcaaa
     4741 cgcataagct aaatacagcg aaggaatgta tcgtaattaa ggaaaaattg aatatacaaa
     4801 acaaggtgcc ctcgagatgg attagcattc tggagaattt cacctgatat caaactcaaa
     4861 taccccttag caaaaaagca aatgaaagca ggaaccctta aaaatccggg aacctgccaa
     4921 acgtcctcgt taagtaatat gagggggggg tgaaccccct catattactt attatcgtca
     4981 tccactactc ggtaatcggc gtcgacgaca ttttcatctt gagtagtttc gtttgagcta
     5041 ccagcctggc tggaagcaga agcttccgat tgagcagtct tttgatagag ctcctgcgcc
     5101 atggcgtgag aagcattttg caaatcatcc atttttcttc tgatatcggc tatatcatct
     5161 ttattcatgg cttcccttaa agcattgaga gcgttctcta cttgagccct ggtttcagaa
     5221 gatagtttgt ctcctgattc ccgcaggttt ttctctacgg tgtaaatcag attatcagcc
     5281 tgattgcgaa cttcaacttc ttcttgtcta cgacggtctt cttcagcatg gagttctgct
     5341 tctctaatca ttcggtcaat atcctcttta gaaagaccgc tcgaggcttt aatggtaata
     5401 gtctgttctt tgttagtagc caggtcttta gctgagacgt gcaaaatacc atcagcatcg
     5461 atatcaaatt ttacttcaat ctgaggtacg cctcgtgggg caggaggaat accagtcaac
     5521 tggaatctgc ccagagagac attgtcccgc gccataggac gttctccctg caagacgtga
     5581 acctctactg tagtctggtt gtctgcagca gtagtgaaaa tctggctttt agaaacagga
     5641 atagtggtat t
//
LOCUS       DAOPSS010000098         5610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_11760_length_5610_cov_7.594599, whole genome shotgun sequence.
ACCESSION   DAOPSS010000098 DAOPSS010000000
VERSION     DAOPSS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11760_length_5610_cov_7.594599"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKN31_06310"
     CDS             <1..630
                     /locus_tag="PKN31_06310"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOA99571.1"
                     /translation="LLEGEGIKRATLDSVVVSCVVPPVLGALEYLFREKWLLPYLFVG
                     PGVKTGLSLRVEAKEVGADRVVNALAASSLYGGDLIIVDFGTATTFCAVTKNREYLGG
                     VIAPGISISAEALYEKTAKLPRVDIEVPSHIVGKNTVEAMRTGIFFGHIGLCREIVKR
                     MKEEFSLEAKVIGTGGWSVFLHRYCDFIDVFNPVLTLEGLRIIYELNQE"
     gene            611..979
                     /locus_tag="PKN31_06315"
     CDS             611..979
                     /locus_tag="PKN31_06315"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOA99572.1"
                     /translation="MSSTKNEEQELAIVGGQSKIRGSFQGGNFLIVEGEFAGSIQCQE
                     LVITRRGKIEAWVEVENMEVWGEVNGFSRVKKVICRKTSRIGGCIMGGGIALEPGVVI
                     EGALAFEKAEDVENEGKESS"
     gene            957..1862
                     /locus_tag="PKN31_06320"
     CDS             957..1862
                     /locus_tag="PKN31_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOA99573.1"
                     /translation="MKEKRVVSASLGSSKRNHRAEVELLGCRVFIERIGVDGDKKKFV
                     ELLQSLDGKVDAIGLGGADLYLRAGQYHYEVKDVARLVSVLRETPIVDGGELKDTWER
                     EVPRYLRESEGIEFKGKTALIMSGMDRYGLAEGLHQEGCNLLIGDLPFAVGVPVALRK
                     LGTLRFLSVLMMPVLRRLPIDVLYPTGEKQEEHQPRFSGYFEKADIIGGDFLYIWRFM
                     PDRLPGKMIITNTVTSQDISEMKEKGVKVLVTTTPEMNNRSFGTNVLQAIFVAVLEKK
                     PEDIKPEEYLELMKELNIKPRVIKL"
     gene            1907..2425
                     /locus_tag="PKN31_06325"
     CDS             1907..2425
                     /locus_tag="PKN31_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOA99574.1"
                     /translation="MEGKVLTIRKAQLEDLQQITEIYNEAVLKTISTFHLYPRSWEEQ
                     VGWFKSHGDKHPLLVAEENGFVIAWACLSPYSDREGYQHTVTDSIYVREEFRRQHIGY
                     ELLSFLAEEAQKLGYHSVVAFIARENLASIRLHEKVGFLYRGELKEVGFKFGKWVDVC
                     LYQKMLEGGEGR"
     gene            2371..2961
                     /locus_tag="PKN31_06330"
     CDS             2371..2961
                     /locus_tag="PKN31_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HOA99575.1"
                     /translation="MGRCVSLSENAGGGRGKIKSREILEKKFPKKEVKTRPGRKGQEV
                     YYLETASVIRRLNEAWEGDWSFEVKDKGIDREAGCVWVLGRLTCGGVVKEQFGSKIIS
                     LSSDGAMVDLGSDLKAATSDALKKCATLLGVGIYLYEEEDEALSRRASQKQKDFLRDL
                     LKEKGKAVDEEMLKTISSEEASRLIDECLKKEESKK"
     gene            2988..3551
                     /gene="amaP"
                     /locus_tag="PKN31_06335"
     CDS             2988..3551
                     /gene="amaP"
                     /locus_tag="PKN31_06335"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOA99576.1"
                     /translation="MNFWGKFFTFIVGIILIGMALGMIAITFNLVPLLTLQEWVVATY
                     LFFSQNIFSQALGVICTVILVLVALLIFRLLIPKREKSLIYHTEQGDIRVSFDSLQSL
                     AQEALRGFDEILQSRAEVEKVGNEARLILHLIVKPEASIPELSPIIQNRVKEKIERQT
                     GVIVKEVKLTIDLKSEDKEEKEEEETV"
     gene            complement(3510..4139)
                     /locus_tag="PKN31_06340"
     CDS             complement(3510..4139)
                     /locus_tag="PKN31_06340"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOA99577.1"
                     /translation="MASIAISLERWGAKLFPRRGIIWGVLGLMIFLLAHPHYPLFSWG
                     IALTCLGEGLRIWGVGYIKNYRGPMQEAERLTTAGPYAYLRNPLYLANGIIGTGIAII
                     SGLWWSIILFWLAFYLLYFPIIRAEESFLENKFGESYKTYAREVPRFFPQLSPYPERN
                     GTFSWRAIEEKEIITLFTLGAAIFLLYLRGFGFLKMLNRLFLFFLLFIL"
     gene            complement(4115..5113)
                     /locus_tag="PKN31_06345"
     CDS             complement(4115..5113)
                     /locus_tag="PKN31_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOA99578.1"
                     /translation="MVIEKVGKISPSALERLVFPFQGKNREEVIWGAQLGRDCGVVQL
                     GRELISLTCDPITGSSFHLGLLATQVVVNDLICSGAEPVALLVTLIFPPGITENEIET
                     IMREIDEAGKELGVSIIGGHTEISEAVNRSIIHCTGLGWIKDGYLPDVSKIEAGDYIV
                     MTKGAGIEGTAILFSEKEKEWQNLLDEETINKGKEFWKMMSVFQEGKIALPFHPHALH
                     DATEGGVLGAIWEACMSRGLGFEIEEGKIPVYPETRVLAQLLDLDPLKLISSGTLIIF
                     TPQPLPLLQALREIVPAEVIGRVVKDPTKVLIRQNGEKEEIKECPQDELWRVLQSA"
     gene            complement(5091..5519)
                     /locus_tag="PKN31_06350"
     CDS             complement(5091..5519)
                     /locus_tag="PKN31_06350"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOA99579.1"
                     /translation="MRGLEFLDKWSKSLSLKSRHDFLSRFLRECPVEVFLPPLVTLEG
                     NIETPGLVRVEGTLKGKVKCPVIIIAEHARATVEVEAQCVYVEGFLRGIVNSSFFYLS
                     SSGKVEGEIKTEILFVDQGAWMKGKLLIEKGRNDGDRKSG"
BASE COUNT         1599 a         1065 c         1372 g         1574 t
ORIGIN      
        1 ctcctggaag gggaaggaat taaaagggct actttagaca gtgtggttgt ttcctgtgtg
       61 gttccaccag ttttgggtgc tcttgagtat cttttccggg aaaagtggct ccttccttat
      121 ctttttgtag gaccgggagt taaaactggc ctctctcttc gggtagaggc taaagaagtg
      181 ggagcagacc gggtagtcaa tgctttagct gcttcttctc tctatggagg agatttaatt
      241 atcgtggact tcggcactgc caccactttt tgtgcggtaa ccaaaaacag ggagtatctg
      301 gggggcgtga ttgctccggg aataagcata tcggctgaag cactttacga gaagaccgcc
      361 aaactccccc gggtggatat tgaagttcct tcccacattg taggtaaaaa tacagtggaa
      421 gctatgcgta ccgggatttt ttttggccat attggcttat gccgggagat tgtgaaaagg
      481 atgaaagaag aattttccct ggaggcaaag gtaataggca caggggggtg gagtgttttt
      541 ttgcaccgtt actgtgattt cattgatgtt tttaacccgg ttttaactct ggaaggtttg
      601 aggattatct atgagctcaa ccaagaatga agaacaagaa ttagccattg tagggggaca
      661 atctaaaatt agaggtagtt ttcaaggagg gaattttttg attgtggagg gggaatttgc
      721 tggttcaatc cagtgccaag agctggtaat aacgagaaga gggaagatag aagcctgggt
      781 ggaagtggag aatatggaag tgtggggaga ggtcaatggt ttttcccggg tgaagaaagt
      841 aatctgtcgc aaaacctctc gcataggggg ttgcattatg ggaggaggta tcgctttaga
      901 gccaggggtc gtaattgaag gagcattggc ttttgaaaaa gcggaggatg tagagaatga
      961 aggaaaagag agtagttagt gcgagcctgg gttcctcgaa aagaaatcat cgtgcagaag
     1021 tagagctttt gggctgtaga gtctttatag agagaatagg agtagatgga gataaaaaaa
     1081 agtttgtaga gttattgcaa agtttggatg gcaaagtgga tgctattggt ttagggggag
     1141 cagacctcta cctgcgggca ggacagtatc actatgaggt aaaagatgta gcgcgattgg
     1201 tctctgtgct ccgggaaacg cccattgtgg acggaggaga gttaaaagat acctgggaga
     1261 gagaagtccc tcgctatttg cgagaaagtg agggaataga atttaaaggg aaaaccgctt
     1321 taataatgag cggaatggac cgttacggat tggccgaagg tcttcatcag gaaggatgta
     1381 atcttctcat tggagatcta ccttttgcgg tgggcgttcc tgtggctctg cggaagttag
     1441 ggactctccg ttttttgtcg gtattgatga tgcctgtttt gcgccgtctt cccattgatg
     1501 ttctttatcc tacgggggaa aagcaggagg agcatcagcc tcgttttagt ggttactttg
     1561 aaaaagcaga cataattgga ggagattttc tttatatctg gcgttttatg cctgatcgtt
     1621 taccgggtaa aatgataatc accaatactg ttacctcgca agatatatcg gagatgaaag
     1681 aaaaaggagt aaaggttctg gttaccacta ccccggagat gaacaatcgt tcttttggta
     1741 ctaatgtttt acaggctatt tttgtggctg ttctggaaaa gaagcccgaa gatataaaac
     1801 cggaagaata tttagagttg atgaaagagt tgaatattaa gccccgagtg ataaaattat
     1861 gacctgtctt tataaaaagg ataaaaagat atacaatatt gaagctatgg aaggaaaagt
     1921 tttgactata agaaaagcgc agctggaaga ccttcagcaa atcaccgaga tttataacga
     1981 agcggtattg aaaaccatct ccacttttca tctctaccct cgttcctggg aagagcaagt
     2041 agggtggttt aaaagccatg gagataagca tccactttta gtagcagaag aaaatggttt
     2101 tgtgatagcc tgggcgtgtt tgagccccta ttctgataga gagggttatc agcatacggt
     2161 tacggactct atatatgtaa gggaggaatt tcgtcgtcag catattggct atgagctttt
     2221 gagttttctt gcagaggagg ctcaaaagct gggttatcat tcggttgtag cttttattgc
     2281 ccgggaaaat ttagccagta tcaggcttca tgagaaagtg ggttttttgt accggggaga
     2341 attgaaagaa gtaggtttca aattcggtaa atgggtagat gtgtgtctct atcagaaaat
     2401 gctggagggg ggagagggaa gataaaaagc agggagattt tagagaaaaa attccctaaa
     2461 aaagaagtaa aaactcgccc ggggagaaag gggcaagagg tttattatct ggaaacagct
     2521 tcggtgattc gaagattgaa tgaggcctgg gaaggtgatt ggtcttttga agtaaaagac
     2581 aaaggcatag accgggaagc cggttgtgtg tgggttctgg gaagattgac ctgcggggga
     2641 gtggtaaagg agcagtttgg ctctaaaatt atctctctga gctctgatgg ggctatggtt
     2701 gacttaggca gcgacctgaa ggcagcaaca agtgatgcgc tgaaaaaatg tgctaccctt
     2761 ctgggagtgg ggatttacct ttacgaggag gaagacgaag ctctttctcg ccgggcttcg
     2821 caaaaacaga aggatttttt gcgtgatctt ttaaaagaaa agggcaaagc agttgatgaa
     2881 gagatgctca agaccatctc ttctgaggaa gcttctcgtt tgattgatga gtgcttaaag
     2941 aaggaagaga gtaaaaaata actgaataag gagaaggagg gttggctgtg aacttttggg
     3001 gaaagttttt cactttcatt gtaggaataa ttctgatagg tatggctctg gggatgatag
     3061 ctataacctt caatctggtg cctcttctta cccttcagga atgggtggtt gccacttatt
     3121 tgttttttag ccagaatatt ttctcccagg ctttaggagt tatatgtact gttattctcg
     3181 ttctggtggc ccttttaatt tttcggctgt taatacctaa acgggagaaa tcccttatat
     3241 atcacaccga gcaaggggat ataagagttt cttttgattc gttgcagtct ctggctcaag
     3301 aagctctgcg gggttttgat gagattttgc aatcccgagc agaagtagaa aaagtgggga
     3361 atgaagcccg tttgattctc catcttatag taaaacccga agcctccatt cctgagcttt
     3421 cgcccatcat tcaaaataga gtaaaagaaa aaattgagcg ccagaccggt gtaattgtta
     3481 aagaagttaa gctgactatc gacttaaaat cagaggataa agaggagaaa gaagaggaag
     3541 agacggttta acatttttaa aaaaccgaaa ccccggagat agaggagaaa gatggcagct
     3601 cctagagtga aaagagtaat tatttctttt tcctcgattg ctctccagga aaacgttcca
     3661 ttcctctccg ggtaaggtga caattgaggg aaaaaacggg gcacttcccg ggcataagtt
     3721 ttatagcttt ctccaaattt gttttctaaa aagctttcct cagcccggat aattggaaaa
     3781 tataaaaggt aaaaagcgag ccagaacaaa attattgacc accataaacc agagatgatg
     3841 gctatgccag ttccaattat accgttagcc aaatagagcg ggttacgcag ataagcatat
     3901 ggtcctgcgg tggtcagcct ttctgcttcc tgcatggggc cgcggtagtt tttgatataa
     3961 cccactcccc agattctcag tccttctcct aagcacgtta gagctatccc ccagctaaat
     4021 aagggataat gagggtgagc aagaagaaaa atcatcaatc ccagcacccc ccagatgata
     4081 ccccggcggg ggaaaagttt agcgccccat ctttctaagc tgattgcaat actcgccata
     4141 gttcgtcctg aggacactcc tttatttctt ctttttcccc gttctgacgg attaaaacct
     4201 tggtcgggtc ttttactact cttcctatta cttctgccgg tacaatttcc cgaagagctt
     4261 gaagaagtgg taagggttga ggggtgaaga ttataagagt tccgcttgaa atcaacttca
     4321 gggggtctaa atccaggagt tgagctaaaa ctctcgtttc aggataaacc gggattttcc
     4381 cctcttctat ttcaaagcct aaacctctgc tcatgcaagc ttcccagatg gctcctaaaa
     4441 cccctccttc tgtggcgtca tgaagagcgt gaggatgaaa aggaagagca attttccctt
     4501 cctggaaaac gctcatcatt ttccagaact cttttccttt gtttattgtt tcctcatcca
     4561 ggagattttg ccattctttt tctttctcag aaaataagat agctgtgcct tcaattcctg
     4621 ctcctttggt catcacgata tagtcaccag cttctatttt actcacatcg ggaaggtagc
     4681 catcttttat ccacccgaga cccgtacagt gaatgataga cctgtttact gcttccgata
     4741 tctcggtatg tcctcctata atactaactc ccaattcttt tcctgcttca tctatttctc
     4801 tcattattgt ttctatttca ttttctgtga ttccgggagg gaaaataagg gtgacgagaa
     4861 gagccacggg ctcagctcca gaacaaatca aatcattcac taccacttga gtagctaaaa
     4921 gtcccagatg aaatgaagaa ccagttatag ggtcgcaggt gagagatatt aactctcttc
     4981 ctaattgcac cactccacag tctcttccca gttgtgctcc ccagattact tcttctctat
     5041 tttttccttg aaaagggaaa accaatctct ccagagccga cggagagatt ttacccactt
     5101 tttctatcac catcgtttct tcctttctct attaataatt tgcctttcat ccaggctccc
     5161 tggtctacaa atagtatctc agtttttatc tctccctcta cttttccaga agaagaaaga
     5221 taaaaaaagg aagaattaac gatacctcgc aaaaatcctt ccacgtagac gcactgggct
     5281 tctacctcaa ctgttgcccg ggcatgctct gctataatga taaccgggca ctttacctta
     5341 ccttttaaag tcccttccac ccgcacgagg ccaggcgttt ctatatttcc ctccagagtc
     5401 accagtggag gaaggaaaac ctctaccggg cattcccgca aaaaacggct caaaaaatca
     5461 tgacgggact ttaaagataa agacttactc cacttatcta agaattctaa cccgcgcatt
     5521 tgtcagtatt atatcagaag aatatcagaa gagaaccatg gggagtaagc ccaaaagaga
     5581 aaaacaaaaa agaaaaaaat ttctcttctt
//
LOCUS       DAOPSS010000099         5604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_11780_length_5604_cov_6.854388, whole genome shotgun sequence.
ACCESSION   DAOPSS010000099 DAOPSS010000000
VERSION     DAOPSS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11780_length_5604_cov_6.854388"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..1358
                     /locus_tag="PKN31_06355"
     CDS             396..1358
                     /locus_tag="PKN31_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA99580.1"
                     /translation="MKIGVFLVLFQNEPLEKALDYVKGIGAEAVEIGTGGYPGNAHLN
                     PEEFLAKEDNIKRLKEAVASRGLEISGLSCHGNPVHPNKEIAEKHHRDFENTVLLAEK
                     LGVKNVITFSGCPGDSENSRYPNWVTCPWPNDFLEVVNWQWEEKLIPYWKKAGQFARD
                     HGVRVAFEMHPGFCVYNPETLLKIRSAVGDVIGANFDPSHLFWQGIDPLLALRALEGA
                     VYHVHAKDTKIDPKNSPVNGNLDTKPYTQESQRSWIFRTIGYGHDLGFWKDFVSTLRL
                     IGYDEVLSIEHEDSLMSGREGFEKAVALLKEVVIKEKTGEAWWV"
     gene            1527..1724
                     /locus_tag="PKN31_06360"
     CDS             1527..1724
                     /locus_tag="PKN31_06360"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOA99581.1"
                     /translation="MKDITPSEEVIKWFSRDPPKRNEFKQKYFQELDHKKELVSLIIQ
                     KAMEEKFILSYGERKKNLITE"
     gene            1829..3025
                     /locus_tag="PKN31_06365"
     CDS             1829..3025
                     /locus_tag="PKN31_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA99582.1"
                     /translation="MSKSKEELYQERLKRYTKAMKNEKPDRIPIRPFVAEFTALYAGY
                     TCQEVTHDYEKAFLAVRKCAADFDWDAIVGNMVYVWTGLTQAINLTYYAVPGIDIPPD
                     VGFQYREPPEEEAFMKSEEYDDLIKDPTGYLFNIWLPRVSRDIVPPKESATFRHNLAL
                     LKGGMAMMQYFDALGRQNQLLREETGTVSAISGILKAPLDILADKLRGYYGLARDLME
                     RPDKVLAACEALMPHLLQVALSGADPEKNVPVAMWMHRTSVPFISPQHFQDIYWPTTK
                     PILEELWNNGHQTLLYAEGNWDFHLSTFRELPAGSVVFHVDRSDIFEAHRVLGDKFCI
                     SGGIPNTLLSIGAPEEVRDYCKKVIDGVARDGGYVMDASAIVQNDARVENVKALTDFT
                     REYGQY"
     gene            3035..3196
                     /locus_tag="PKN31_06370"
     CDS             3035..3196
                     /locus_tag="PKN31_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99583.1"
                     /translation="MEEMKRNPGVCIPWEEKVKELPPISGDKELARRVWEEVDFLGYL
                     FIWHCLVSF"
     gene            complement(3193..4110)
                     /locus_tag="PKN31_06375"
     CDS             complement(3193..4110)
                     /locus_tag="PKN31_06375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOA99584.1"
                     /translation="MVNYLIASNIIFFLATIFSGGFTLSNLLRWGAKFGPLIANGEWQ
                     RLLICIFLHGNLWHLFFNLYALYYLGRMAEEIYGYRKFFGIYIVSGLSGSILSYLLNY
                     SQIGVGASGAIFGLAGAIFASGLKYRHTSLHQVGMNLLPFILINLLIGAFSPAIDNAA
                     HLGGVLSGMLLGWIVSPGTSWIRWKRTLEEVLYWVTIAFVVFAMLTFFVPNLASGKAS
                     IDKIVTFHNEAQKILLKIELGFPPSPLEVEKLSPPDRKAREIKELLLTFLEEGGKNSV
                     LQREIEEKFLSWREEILDKYQGIILEKSP"
     gene            complement(4114..4836)
                     /locus_tag="PKN31_06380"
     CDS             complement(4114..4836)
                     /locus_tag="PKN31_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HOA99585.1"
                     /translation="MISQFLEEKQVNMVAAVLAVIFVVISIILGVAGGFMFAVGDLTG
                     ALASWGIVALFGLIGLILQAIVLYQWSAVINQNLSNTQQMFLYLKERIEDPLRGEIGF
                     FANRMEEFTIPTWPYWVYLAMYIISLFTGWYALLFNLLGFIFLAFYINNIFKSTSKVS
                     NLKNKVYHYLKEEKGVSLSGEVYRIPSRNIITFIILSVITLSIYWLYLLVKLSSEINN
                     YVKSDEKMRKEIEEVFRAAAQA"
BASE COUNT         1650 a         1019 c         1286 g         1649 t
ORIGIN      
        1 cgcttcgtaa ccatttttaa ttctctttta cactttgccg tttcgggtgt caattgcgac
       61 tccctccttt gcttgtgtgc caactctgct ttttctcccc tcggaaggag cttttccttc
      121 ttttcttctt ccccttgtgg gagtgcccgg gtcacctgcg agatcaatga ttggacaggg
      181 tgggtaaggg agggggaaaa gaaagggatt tataaggggg cgaaccccct tataaccgat
      241 agaggtattc agagggatgg atgaggagtg atagggaaaa agcgataggg gataccatct
      301 tgtgtctctg tttcaggaaa aacaaaatat catctttttt caataacttg tttatgatat
      361 gctaaattaa attctattaa taggaggaaa aagtaatgaa gataggagta tttttggtac
      421 ttttccaaaa tgagcctctg gagaaagctc ttgattatgt taaagggata ggagcagaag
      481 cggtggagat tggcaccgga ggatatccag gcaatgctca cttaaacccg gaagagtttc
      541 ttgctaaaga agataatata aaaagactta aagaagcagt agcttcccgg gggttggaaa
      601 ttagtggtct gagctgtcat ggtaacccgg ttcatcccaa taaagaaata gctgaaaagc
      661 atcatcggga ttttgaaaac acagttcttt tagctgaaaa actgggagta aaaaacgtga
      721 ttactttctc cggttgcccg ggtgattcag agaattctag ataccctaac tgggttacct
      781 gcccctggcc caatgatttt ttagaagtag ttaattggca atgggaggag aagctgattc
      841 cttactggaa aaaagctggt caatttgctc gagatcacgg ggttcgggtg gcttttgaga
      901 tgcaccctgg tttttgtgtg tacaatcccg agactttatt aaaaatccgt agcgcggttg
      961 gagatgtaat cggggctaat tttgatccca gccatctttt ctggcagggg atagaccctc
     1021 ttttagccct gagagctctg gaaggtgcag tttaccatgt tcacgctaaa gatactaaaa
     1081 ttgatcctaa aaatagcccg gtaaatggaa acctggacac taaaccctac actcaagaga
     1141 gccagaggtc ctggatattc cgaaccattg gctatgggca tgatctgggt ttctggaaag
     1201 attttgtgag cactttgcgt ctcatcggct atgatgaagt tttgtctatt gagcacgaag
     1261 attctcttat gtcggggagg gaaggttttg aaaaagcagt ggctcttttg aaagaagtag
     1321 tgattaaaga aaaaactggt gaagcctggt gggtgtagtt tttttgagag tgcctgttca
     1381 tttttacgaa tttagggagt taaaaattag tcataaatat taagagagct tatgatgaaa
     1441 cagatgaagg cgatggtttt agaatattgg tagatagatt ttgccctcga ggtttaaggt
     1501 agggaagagc aaatattgat ctatggttga aagatattac cccaagcgaa gaagtgataa
     1561 agtggttttc tcgtgatccg ccaaagcgga atgaatttaa acaaaaatat tttcaggagt
     1621 tagaccacaa aaaagagctg gtttcgctta tcattcagaa agcaatggaa gaaaaattta
     1681 tcctttcata tggagaaagg aaaaaaaatt taataacgga atagttttaa aaaagtatat
     1741 cgaaactaca atgggatgag gtttaaagat gacagtgata gttggttctt ctgtgaggaa
     1801 aagattagat agtaatagga ggtaaaatat gtctaaatct aaagaggagc tctaccagga
     1861 acgcttgaag aggtatacaa aagcgatgaa gaatgagaag ccagacagga ttcccattcg
     1921 cccttttgta gccgagttta ccgctcttta tgctggctac acctgtcaag aagtgaccca
     1981 cgactatgag aaagcttttt tggcggtaag aaaatgtgct gctgattttg attgggatgc
     2041 aatagtgggt aatatggtat atgtgtggac tggtttaact caagctatca atcttactta
     2101 ctatgctgtg ccaggtatcg atattccccc cgatgtgggt tttcagtacc gggaacctcc
     2161 agaggaggaa gcttttatga agagtgagga atatgatgat ctgattaaag accctactgg
     2221 ttatcttttc aatatctggc ttccccgagt ttcccgggat attgttcctc caaaagagtc
     2281 agccactttt cgtcataacc tggctttgtt gaaaggaggg atggctatga tgcagtactt
     2341 cgatgctctg gggaggcaaa accagctttt gcgagaggaa accggaacgg tttctgctat
     2401 ctctggaatc ttaaaagctc cccttgatat cttagctgat aaattacggg ggtattatgg
     2461 cctggctcga gacttgatgg agcgtcctga taaagtatta gccgcttgcg aagctttaat
     2521 gcctcatctt ctccaggtgg ctctttctgg agcagacccg gaaaagaatg ttccggtggc
     2581 tatgtggatg catcgtacct ctgttccttt tatcagtcct cagcattttc aagacatcta
     2641 ctggcctacc actaaaccta ttttagaaga actgtggaac aacggccatc agactctcct
     2701 ttatgctgaa ggcaattggg actttcatct ctctactttt cgagagttgc cggcagggag
     2761 tgttgtcttc catgtggata ggagcgatat atttgaagcc catcgggttt tgggggataa
     2821 attctgtata agcgggggca tacctaatac ccttttatca ataggagcac ccgaagaagt
     2881 tcgggattat tgcaaaaaag tgattgacgg agtagctcga gatggtggat acgttatgga
     2941 tgccagtgct atcgtccaga atgatgctcg agtggagaat gttaaagcac tgactgattt
     3001 tacccgagaa tatggtcaat attaggagga taaaatggaa gagatgaaga gaaatccggg
     3061 agtttgtatt ccctgggagg aaaaagttaa ggaattgccc cctataagtg gggataaaga
     3121 attagcgcgc cgtgtttggg aggaagttga tttcctgggt tacctattta tctggcactg
     3181 tttggtctct ttttagggag atttttccag aataattcct tgatatttgt ctaaaatttc
     3241 ctctctccag gataggaatt tttcctcaat ttctctctgg agcaccgagt tctttcctcc
     3301 ttcttcgaga aaggtgagga ggagctcttt aatctctctg gcttttcggt cgggaggaga
     3361 aagcttttct acctcaagtg gtgagggagg aaaccctaat tctatcttta ggagtatttt
     3421 ttgtgcttca ttgtggaaag tcacaatttt gtctatagat gctttccctg aagccagatt
     3481 gggaacaaaa aaagtcaaca tggcaaaaac tacaaaggca atggttaccc agtagagtac
     3541 ttcttctaaa gttcttttcc accgaatcca ggaagtgcca ggacttacta tccacccgag
     3601 aagcatgcca ctgagcactc ctcctaggtg agcggcatta tctatagcag gagaaaatgc
     3661 gccaatgagg aggtttatca ggatgaaagg caggagattc attcctacct ggtgaaggga
     3721 agtatggcga tatttcaggc cactggcaaa aattgcccct gctaagccga atatggcacc
     3781 acttgctcct actcctatct gggagtaatt cagtaaataa gataagatgc taccactcag
     3841 tcctgagacg atgtagatgc caaagaactt gcgatatcca tatatttctt ctgccatacg
     3901 tcctaaataa tagagcgcat ataaattgaa aaataagtgc cacagatttc cgtggaggaa
     3961 aatacagata agaagtctct gccactcccc attagcaata agggggccga atttagctcc
     4021 ccagcgtagt aaattgctca aggtaaagcc accgctaaaa atggtggcta agaaaaatat
     4081 aatgttagag gcaatcagat aattgaccat taattaagct tgagcagccg ctctaaaaac
     4141 ttcttctatt tcttttctca ttttctcatc gctctttacg taattattga tttcactgct
     4201 tagtttaacc agcaggtata accagtaaat agataaagtg atgacactta aaattataaa
     4261 agttattatg ttgcgtgagg gaatgcgata aacctcgccg gagagagaca ctcctttttc
     4321 ctcttttaaa taatggtaaa ctttgttttt aaggttggac actttacttg tagatttgaa
     4381 aatattgttt atataaaaag ctagaaatat aaatcccagc aggttgaaca ggagggcata
     4441 ccaaccggta aagagactga tgatatacat tgctaagtac acccaataag gccaagttgg
     4501 tatagtgaat tcttccattc gattggcaaa aaatcctatt tctcctcgca gaggatcctc
     4561 tatgcgctct tttaagtaga gaaacatctg ctgggtatta cttaaattct ggttaattac
     4621 tgctgaccac tgatatagga ctatggcctg gaggatgagt ccaataaggc caaagagagc
     4681 aactatcccc caggaggcaa gagctcctgt tagatctccc acagcaaaca taaatccgcc
     4741 tgctactccc agtataatag aaataaccac gaaaatcact gctaatactg ctgctaccat
     4801 atttacctgt ttttcttcta aaaattggga tatcattata ataaccctcc ttttacttta
     4861 tttctttcat tttaacattt atttttgtac aatacgaaaa tttttcttta aaaatgatct
     4921 gctttttgaa aaatatcagt tctgaacaaa gttaggttca ttaattatat aattgtgcaa
     4981 gcttgaagag ccaggcttag aaacttcacg gttagaacaa aattgctcct caaaactcaa
     5041 atggagggat tttgaaatgg ttcactctgg gtgggagttt ttgtctgatt gttttatgat
     5101 agaacactca gtagtttgct taagttcagg aaaaactgta aacagagagg attaaacaac
     5161 ctaccttaaa atctcttttt gttttgacat cagggccacc ttaaggctta taaaactcct
     5221 ttctcaaaaa ctgtatggct atgtgatggt gcaggagaaa gagtcgagta caagggagga
     5281 aggttaccac acttttttag tggtcataaa agtagggggg ttttcaaaca tccccaggtg
     5341 ttttacccca gagctgtgcc catgccaatt atagcaaatg cagtgatgat ggtgtcaaga
     5401 tttttaaggt tctctatcga aagagatggg aaggtttgta aggattatac ttcctctttc
     5461 caggccagct ttgactactc tgggaatttt tactcaatgt atggaataac tatcgttatc
     5521 ctctggtgtt cattaatagc ccgggcaaga gaacacttac tctgggaata actttgttca
     5581 ggggagatat ggatgctcag tgga
//
LOCUS       DAOPSS010000100         5592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_11805_length_5592_cov_8.658479, whole genome shotgun sequence.
ACCESSION   DAOPSS010000100 DAOPSS010000000
VERSION     DAOPSS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11805_length_5592_cov_8.658479"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKN31_06385"
     CDS             complement(<1..322)
                     /locus_tag="PKN31_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOA99586.1"
                     /translation="MSGEVKSGVNRFREVLEKVGLSSRIVEFSCSTRSSEEAAQAIGC
                     EIGQIVKSLVFEGAHTHRPFLVLASGENRVNEKQLECLVGEPVCKGDAQFVREKTGFA
                     IGGVP"
     gene            842..1837
                     /locus_tag="PKN31_06390"
     CDS             842..1837
                     /locus_tag="PKN31_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99587.1"
                     /translation="MPIKNIWITYSQEILENQEKSYYKTRSELKGKTLTMIEEALQEA
                     GWEVNKISVDLPLPQLTEFFSQKIDLIFNLTICVSGIYNQAFLPLLLDANNIPYLGSN
                     SVVHSLCLDRSLSKLCLRGIGIPTPSYFIWSSKEEIPENLDFPLIIKPRFRSHQEIIS
                     AQSVVSNQEELERELQRVFSETRDEVIAEKFLTGREMMIGIWGNGKSASPLPIMEVNV
                     SRQVLLWDFQTKNDKGYVEDVLCPAPLSEEQKNQIEKMALKIFQELHMRDYATFKLIF
                     SEKEEIPFFFELNSLPSLYYRHSAFPEMCNASGIDYKTMVQKLARIAEERVARKL"
     gene            1834..2937
                     /locus_tag="PKN31_06395"
     CDS             1834..2937
                     /locus_tag="PKN31_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOA99588.1"
                     /translation="MNQNQELTFAVLGAGHGGQALAAYLSLKGFRVNLFNRTPERINS
                     IKLMGGMQVEGEIQGFVPLNLVTSDIEEAIRGVKVIMIVVPATGHRYLAERLSNYLED
                     GQIIVLNPGRTGGALEVKQVLKERECQAKVIIAEAQTFIFASRISGPAQVKIFRIKNS
                     VPVAAIPAHQTVEVVQTLRKALPQFVPEDNVLKTSFNNIGAVFHPTITILNAGRIEST
                     HGGFDYYIEGVTPSVALILEEVDKERVKVGEALGIRAITAREWLYSAYDAHGRNLFEA
                     IQNNPGYYGIKAPSTIFHRYLTEDVPASLVPISSIGHMLGTPTPTIDTMIHLACILHG
                     RNYWQEGRTVEKLGLEGLSVKEIREMVVMEEEE"
     gene            2934..4580
                     /locus_tag="PKN31_06400"
     CDS             2934..4580
                     /locus_tag="PKN31_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOA99589.1"
                     /translation="MKTILGASLGNCVHVAGIYRFLSLAEQYGYQAHFLGPAIPTEKL
                     IEAIKEKKPERVIVGYRLSEEAAQNIFQELKEALQKNQLLGKFILILSCTPALENVAR
                     QTGIFDYIFTGKERFEEIVQSITGHKESSPEEKYPPTLPERIKAKSPFPLLRHHFGLP
                     SLSETIEGVARISESRVIDVISLGPDQNAQEFFFQPHLMNEEKRGAGGVPLRHPEDLK
                     KIYEASRRGNFPLLRCYSGTNNLVEWAHLLKETINIAWGAVPLMWYSQLDGRSQRSLP
                     VAIRENQEAMRAYASLGVPLEVNESHQWSLRDAPDSVAAATFYLAAYNAKKAGARYYI
                     AQYMLNTPPGISPQADLAKMLAKAELVKELEGESFTVFTQTRGGLAHFSVDLNVAKGQ
                     LGASTFLGLFLQPQIIHVVGFCEADHLAHPEEVIESAQIVEGVIKDALFGLPQVQNDP
                     VILENKERIKREAKLILEAIKRIGKEEEDPWASPQVLTQAVEEGILDAPHLLGNPHAP
                     GKVKTRIIDGFLRVVNERGEIITEEERLNPLLQRLRLESL"
     gene            complement(4553..4915)
                     /locus_tag="PKN31_06405"
     CDS             complement(4553..4915)
                     /locus_tag="PKN31_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOA99590.1"
                     /translation="MVEVGNIVEGKVVGITKFGAFVELEDGKKGLVHISEISNHFVKD
                     VNEYLKVNDQVRVKVIGIAPDGKIDLSIKKVNEDRDYEAKLEKSKAVFEQKMNRFLKQ
                     SQEKLTDLKRYNDSKRKR"
BASE COUNT         1656 a         1288 c         1155 g         1493 t
ORIGIN      
        1 gaggaactcc cccgatggca aaaccggttt tttccctcac aaactgagca tctcccttgc
       61 agacgggctc tcccaccagg cattccagtt gcttttcgtt aacccggttc tccccacttg
      121 ctaaaactaa aaaagggcga tgagtgtgtg ctccttcaaa aaccagagac ttaacaatct
      181 gccctatctc gcaaccaatc gcctgagctg cttcttcaga gctccgagta gagcaagaaa
      241 actccactat ccgtgaggat aggcccactt tttctaacac ttctctaaac cgatttactc
      301 ctgactttac ctctccagac acagcatttc ccccttttac tatacatttt acctttctat
      361 ctctactttg caaggcctct ggatgaacac tcgctttatg ttttcatctc aaggaaaaaa
      421 tcgaaaccat ggccggtcac agaattcatt gtaacccttc tctcgatgaa aaagcccgtt
      481 atccagggga aaatagccta ttcctcccct atagttctgg aagctacccc tttagcaagc
      541 gcacccctct ttgcgtggca gcaaattaca tcgtgctaaa agcaaccctc gcaatagcga
      601 aagagaaata ggctgtttta tttctttctt cctcagagct atcttcagct ttttacttac
      661 tctgtagctc ttttattttc caaagttatg aacgaacacc ccctttttta taggtctgtg
      721 gtcataacac acttgagtct ataaggcctc cgaaggctta ttgctgcccc gtccggtcaa
      781 aaatttgcac taagaaacca acttgtgtta tcctatttcg gtaaaagggt aaaaggaagt
      841 gatgcctatt aaaaacatct ggattaccta cagtcaggaa attctcgaaa atcaggaaaa
      901 aagctattat aaaaccagaa gcgaacttaa aggcaaaacc ttaaccatga tagaagaagc
      961 attgcaagaa gcaggatggg aagtaaataa aatcagtgta gacctgccct tgcctcagtt
     1021 aactgagttt ttctctcaaa aaatagactt gatattcaat cttaccatct gcgtttcggg
     1081 catttataat caggcttttc ttcctcttct tttagatgcc aacaatatac cctatctggg
     1141 gtctaattcc gtagtccact ctctttgcct ggaccgttct ctgagcaaac tctgcttaag
     1201 ggggataggc atacccaccc cttcttattt tatatggtct tcgaaagagg aaataccaga
     1261 aaatctggat tttcccctca tcataaagcc ccgttttcgc tctcaccagg aaataattag
     1321 cgcacaatcg gtggtctcca accaggaaga gttagaaaga gaattacaga gggttttttc
     1381 agaaaccaga gacgaagtga tagcggaaaa gttcttgact ggaagagaaa tgatgatagg
     1441 aatctggggc aacgggaaaa gcgcctcccc cttacccatt atggaggtta acgtttctcg
     1501 tcaggttctt ctctgggatt ttcaaactaa aaatgacaaa ggatatgtag aagacgtgtt
     1561 gtgccccgcc cctctcagcg aagaacaaaa aaaccagata gaaaaaatgg ctctcaaaat
     1621 tttccaggaa ttgcatatga gggattatgc tactttcaaa ctgatatttt ccgagaagga
     1681 agaaataccc ttcttttttg agctcaactc tcttccctct ctttattatc gacacagtgc
     1741 cttcccggaa atgtgcaacg ccagtggaat agattataaa actatggtgc agaaattagc
     1801 ccggatcgcc gaagagaggg tggccaggaa actatgaacc agaatcaaga attaactttt
     1861 gctgtgctgg gagcaggaca cggagggcag gctctggccg catatctgag cctgaaagga
     1921 tttcgagtca atctttttaa ccgcactcca gagcggataa actctataaa acttatggga
     1981 ggaatgcagg tcgaaggaga aatacaaggc ttcgttcctc tcaatctcgt gacttctgat
     2041 atagaagaag ctatccgggg tgtaaaagtg ataatgatag tggttcctgc caccggtcat
     2101 cgttatctgg ccgagagatt gagcaattac ttagaagacg gccagatcat agtccttaat
     2161 cctgggcgca ctggaggggc attagaagta aaacaggttc taaaagaaag ggagtgtcag
     2221 gctaaagtaa taatagctga agcacaaaca tttatctttg ccagcagaat ttctggccca
     2281 gctcaggtga agattttcag aattaaaaat agcgtgccgg tagctgctat ccctgctcat
     2341 caaacagtgg aggtagtgca aaccttaaga aaagctcttc ctcaatttgt ccccgaagat
     2401 aacgttttga aaaccagctt caacaacatt ggcgcagttt tccatcccac cataaccata
     2461 ctcaatgccg gtcgtatcga aagcactcac ggaggatttg actactacat agaaggagtt
     2521 accccttcgg tagcattgat tttagaggaa gtggataaag aaagagtaaa agtgggggag
     2581 gctctgggaa taagagcaat caccgcccgg gagtggctct attctgctta tgatgcccat
     2641 ggccgcaatc tttttgaagc cattcaaaat aacccgggat attacggaat caaagcccct
     2701 tctactattt ttcaccgcta cctgacagaa gacgttcctg ccagtcttgt tcctatctct
     2761 tccataggtc atatgttagg aactcccact cccacaattg ataccatgat tcaccttgcc
     2821 tgtattcttc atggaagaaa ctactggcaa gagggaagaa cggtggaaaa attggggctt
     2881 gaaggtttga gtgtcaaaga aattcgggaa atggtggtaa tggaggaaga agaatgaaaa
     2941 caattttagg agcatcgctg ggcaattgcg tccatgtggc tggcatatat cgctttctgt
     3001 ctttggctga gcaatatggt taccaagccc attttttagg cccggctatc cccacagaaa
     3061 aactgataga agcaataaaa gagaaaaagc cagaaagggt gatagtgggc taccggttga
     3121 gcgaagaggc agcccagaat atattccagg agctcaaaga agctttgcaa aaaaatcaat
     3181 tactgggcaa atttatcctc attttaagct gcacccctgc tttagaaaac gtggctcgcc
     3241 agacggggat tttcgattac atatttactg gtaaagagcg ctttgaagaa atagtacaaa
     3301 gcataaccgg acacaaagaa agctctccag aagaaaaata tcctcctacg cttccagaga
     3361 gaataaaagc taaaagccct tttcctcttt tgaggcatca ctttggactg ccttctctat
     3421 ctgaaaccat agagggcgta gctcgaatca gcgaatcccg ggttatagat gttatctcct
     3481 taggcccaga ccagaacgct caggaatttt ttttccagcc tcacctgatg aacgaggaaa
     3541 aaaggggagc aggaggcgtt cctctgcgtc acccggagga tttaaaaaaa atatacgagg
     3601 cttctcgccg ggggaatttt cctctcctgc gctgctatag cggaaccaat aatctggtgg
     3661 aatgggctca ccttttgaaa gaaactatta acattgcctg gggagcagtg cctcttatgt
     3721 ggtacagcca gttagacggt cgctctcaaa ggagcctccc tgtagcaatt cgggaaaacc
     3781 aggaagccat gagggcttat gcttcactgg gagtccctct ggaggtgaac gagtctcacc
     3841 agtggagctt acgcgatgct cccgatagcg tagcagcagc caccttttac cttgctgcct
     3901 acaacgccaa aaaagccgga gctcgttatt atattgctca atatatgctc aacactccac
     3961 cgggcatatc ccctcaggcc gatttagcta aaatgctggc taaagcggag ctcgtaaaag
     4021 agctggaagg agaaagcttt actgttttta cccagactcg aggagggtta gctcatttct
     4081 ctgtagacct gaacgtagcc aaagggcaac tgggggcttc cactttcctg ggtttattcc
     4141 ttcaacctca aatcatacac gtagttggtt tttgtgaagc cgaccactta gctcatccgg
     4201 aagaagtaat agaaagcgct caaatagtag aaggagtgat aaaagatgct cttttcgggc
     4261 ttcctcaggt acaaaacgac cctgttattc ttgaaaataa agagaggata aaaagagagg
     4321 ctaaattaat tttagaagca attaaaagaa taggaaaaga ggaagaagac ccctgggcct
     4381 ctcctcaggt actgacccag gcggtagaag agggaatatt agatgctccc catctactgg
     4441 gcaatcctca cgcaccggga aaagttaaaa cccgaatcat agatggattt ttaagagtag
     4501 ttaatgaaag aggagaaata ataacggaag aagaaagact gaaccccctt cttcaacgct
     4561 tgcgcttaga gtcattgtag cgttttaaat cagtaagttt ttcctggctt tgttttagaa
     4621 aacggttcat tttttgctca aagacagctt tgcttttttc caattttgcc tcgtagtccc
     4681 tatcttcgtt gacttttttg atggaaagat ctattttccc atcaggagca atgccaatta
     4741 cttttaccct tacctggtca ttcaccttca aatattcatt gacatctttc acaaaatgat
     4801 tggaaatttc cgatatgtgc accaagccct ttttaccatc ttctaactcc acaaatgctc
     4861 caaatttggt aatacccacc actttacctt ccactatgtt accaacttca accattaact
     4921 tccaactatc ctcctttaca aaaattgagt ttattataca aacgacgccc tgaagtgtca
     4981 agaattcgag ggctctatgt caacaacaat ctccttttga tttgatatga acgggaagag
     5041 tatttctctc caataaattt ccaacactta aaatcataaa atattctccc ccgcacctca
     5101 accttggtat tatgactttt tacttaatac gaaaactggc aacctggtcg cttaaaatat
     5161 agttaaccgc aaaatccatt gtaattaact ctcacctgat cctcgcctga tacctgatgg
     5221 caaaccataa gacggcaata gggggtaaac cccttatgaa cccctttctt attcgtcacc
     5281 cctgaatgtc tctatcatcc cctcagctgt cattcctgaa tggttctatc aggaatctaa
     5341 attatttaag gacaaaagcc tttggattcc cacctcctcg tcaccccggc aatttttaag
     5401 ccggggtcta aattatttaa agacaagaat atttggattc ccgactaaag atttcgggaa
     5461 tgacgagaga ggaggtcatc actcctgaat ggttctacga ggggtctaaa tcagaggaga
     5521 ggaaaccctc ccttttatgt catcgcgagg agcgattgag gtggcgatct cgcttttctg
     5581 agcctcaaaa aa
//
LOCUS       DAOPSS010000101         5584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_11824_length_5584_cov_7.918430, whole genome shotgun sequence.
ACCESSION   DAOPSS010000101 DAOPSS010000000
VERSION     DAOPSS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11824_length_5584_cov_7.918430"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            481..1527
                     /locus_tag="PKN31_06410"
     CDS             481..1527
                     /locus_tag="PKN31_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99591.1"
                     /translation="MNCFLFVTQGELEEKLSLKLYHKMKEAYPEVEILFLSIDNPGRW
                     QKKGLMEIKPPHSSFEDNISKFKRLTLIHSALASRRKFEEKVVFWGDNPSFLLPLFLL
                     TGKKAIYIPVIKSLRPSLSFWHTVFFRFSSAVLANDYELVRNLRRKNIPAYFVGNLLA
                     DLLSSSAFLFSHGRKPIYALFPREEKFSSDLKFFLDVVEKMSRGDKTYFILTIPRKIK
                     TREAREIGEKRGWHWRRSLEGDIMEGYLEKEGAYLNLTRFRGEALQQCSLALSTDDIS
                     TIQATGMGRKVLPIFNLNPEEVVSLIRNPQYLFECNQHLSSRYGRRGGIEKISAYLLQ
                     GIVEDEDFLKKFSK"
     gene            1542..2393
                     /gene="nadC"
                     /locus_tag="PKN31_06415"
     CDS             1542..2393
                     /gene="nadC"
                     /locus_tag="PKN31_06415"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOA99592.1"
                     /translation="MLGLVPWDVDRRLQAFLEEDLGFADITTQGLGDLGKNTVKAKVT
                     AKKEGVMAGGYFALRVFTLLNSQTRGEAKVFEGQNFSPGEVLLEIEGPARAILMGERT
                     ALNLLQRLCGIATKTQELVKLVEGLPVKVADTRKTSPGLRIFEKYAVRCGGGVNHRLG
                     LYDCALIKDNHIALCGSVKEAIKKIRPALPFTAKIVVECTTLAQVQEALEEKADIIML
                     DNMSVENIREAVKIAKGKATLEASGDINPTTIRKVAEQGVDIISTGYITHHATWIDMS
                     LKMEENA"
     gene            2386..3951
                     /locus_tag="PKN31_06420"
     CDS             2386..3951
                     /locus_tag="PKN31_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA99593.1"
                     /translation="MPDRYDVLIIGNGIAGSSAALFLAEAGKSILLLTKGEDLEDTNT
                     AKAQGGIVFRGEGDNPSFLMKDIMEASAFTSYTASARLISRLGPPLVQKLLIDRLQIP
                     FDRRNGKWDLFREGNHSFRRILHVKDYTGKVIQEYLNKAVLHEPLIEVKKNSLALDLI
                     VSSRDTPHPESRYLPLECLGAYVWEEEAQEVIPILASATILATGGLSEIYRYSSGGPW
                     NTGDGFAIARRAGARLSNMEYVQFHPTLLYTPQYSSSFLISESVRGEGAELISANGEP
                     FMYRYHPRGSLAPRDVVARAIFQEMKIHESNCVYLDFRQKMEPRKIKDTFPTIYEELL
                     QRGIDITQEPAPVAPGAHFACGGVLTDTRGRTTIKRLYAAGEVACSGLHGANRLASTS
                     LLEGLTFGYRAARDIVKHSEELPNFAVLPFREKWGEPPPENILRSLKEIIRINMWENV
                     GLIRERRGLSQSLQILTAMKKEIAHLKEEYGISPALLELENMAETALLVTEEAFKNPL
                     SRGTHFRLDAFDP"
     gene            complement(3921..5381)
                     /locus_tag="PKN31_06425"
     CDS             complement(3921..5381)
                     /locus_tag="PKN31_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOA99594.1"
                     /translation="MSQPFKQYLRWLYPGMRVKRWLFLVIVGVFLISLGVSLIVSTPY
                     FRSIYVLWRNFVFGFLNRYWLAIVFGLAWLVGGVALVVYGLQKMNRSVITAISSLPEK
                     EVVNEVFRKRQLEKGPSIVAIGGGHGLYSLLHGLKDYTSNTTAVVTVFDDGGSSGRLR
                     EEMGVLPPGDIRNCLIALADVEPLMSELFQYRFGEDTGLGGHNFGNLFITALAQITGD
                     FQKAVFESSKILAVRGKVLPTTVQNVFLRAECEDGSIILGESQVGTCGKKIKRIFLEP
                     SVVHTTPEVVKAIEEADLIVLGPGSLYTSVLCNLAVEEVREAILRSPAPLVFVCNITT
                     QPGETDGYDFSDHLQAVFQFIPPQRLNYILVNNGIPSEAGEEELSLSGAEMVRGNGEE
                     IPSSVKVIKADLISYHHPSRHDSQKLAQVLMSILLEEKPSWAFYLTLHNDEILKGLNP
                     VHLVNQWRLRARENGENKKKEEKSAPLYGSKASRRK"
BASE COUNT         1665 a         1288 c         1206 g         1425 t
ORIGIN      
        1 cccttcgttc gtcaccccgg caatctttaa gccggggtcc agaagtatct aaaaaccaaa
       61 gaaaaaagca aaaacttgga ttcccgccta aatttcagga atgactgcgg gggtggattc
      121 ccacctgaat ttcaggcatg acggcgggga ggtagattcc cgataaaatc tcgagaatga
      181 cagagagcgt agtctggcag cgtaaaccaa aaaaacaaag agcgggataa taacgccggg
      241 ggaaagattc agtgttgctg cctcagcttt ttaatcttcc tccctttttc ttacgctgcc
      301 atacgaggcc tgcaataatg gactcaaaag tcgcaaagcg cttattcttt acgccaaaag
      361 agaggttaca atgattttta cagttaacta tattataata gataaaattt gccagtcagt
      421 ttcttataga ggacagaact tcaaaaatgg gagttattaa acttagaaaa ggtggcggta
      481 atgaactgct ttttatttgt tacccaggga gaattagaag aaaaattatc tctaaaactt
      541 taccataaaa tgaaagaagc ttatcctgaa gtagaaattc tctttctcag tattgacaat
      601 ccaggaagat ggcaaaaaaa agggctgatg gaaataaaac cccctcactc ttcctttgaa
      661 gacaatatat caaagttcaa gagattaacc cttattcatt ctgccttagc gagcaggaga
      721 aagtttgaag aaaaagtggt gttctggggt gacaatcctt cttttcttct ccctcttttt
      781 cttttaaccg gcaaaaaagc catatatatc ccggtaatta aaagtctccg tccttcttta
      841 tctttctggc ataccgtttt ttttcgtttt tcttccgcgg ttttggccaa cgactatgaa
      901 ctggtgcgta atttgcggag gaaaaatata ccagcttact ttgtgggtaa tctcttagcc
      961 gaccttctct cctcttctgc cttccttttc tcccacggga gaaagcccat ttatgctctg
     1021 tttcccagag aagaaaaatt ttcttctgac ttgaagtttt ttttagacgt ggtggagaaa
     1081 atgtcgcgag gagacaaaac ttatttcatt ctaaccattc ccagaaaaat aaaaactcgg
     1141 gaagcaagag aaatagggga aaaaagaggc tggcactgga gaagaagctt agaaggagat
     1201 ataatggaag gatacctgga aaaagaaggt gcttatttga accttacccg ctttcggggt
     1261 gaagccttgc aacagtgcag tctggctttg agcaccgatg atatttccac tatccaggcc
     1321 acaggaatgg gcaggaaagt tcttcccatt ttcaacctca accctgaaga agtggtctct
     1381 ttaatacgca acccccaata tctctttgag tgtaatcaac atctctcttc tcgatatgga
     1441 agaagagggg ggatagaaaa aatatccgct tatcttttgc agggtatagt tgaagatgaa
     1501 gatttcctca agaaattctc taaatagaaa ggatgataaa tatgttaggg ttagtgccct
     1561 gggatgtaga ccgaagactg caggctttct tagaagaaga cctgggattt gctgacatta
     1621 ccactcaagg attgggtgac ctgggaaaaa atacagtaaa agctaaggtt acagccaaaa
     1681 aagagggagt aatggctgga gggtatttcg ctcttcgagt attcactctc cttaactctc
     1741 aaacgagagg ggaggcaaaa gtttttgagg gacagaattt ttcccccggg gaagtgctat
     1801 tagaaataga aggaccagcg cgggctatcc tcatgggaga gagaactgcc ttaaacctcc
     1861 tgcaacgcct ctgtggtatt gccaccaaaa cccaagagct ggtaaagctg gtggaaggct
     1921 tgcccgtgaa agtagccgac acccggaaaa ccagcccggg tttgcggatt tttgaaaaat
     1981 atgccgtgag atgtggaggg ggggtaaatc accgcctggg gctttatgac tgcgctttaa
     2041 ttaaagataa ccacatcgct ctgtgtgggt cggtgaaaga agcaatcaaa aaaatccgcc
     2101 ccgctcttcc ctttaccgcc aaaatagtgg tagaatgtac tactttagcg caagtgcaag
     2161 aagccttaga agaaaaagca gatattatta tgttagataa tatgagtgta gaaaatatcc
     2221 gggaagcagt taaaatagct aaagggaaag ccactctgga agcctccgga gatatcaacc
     2281 ctaccaccat tcgtaaagtg gcggagcagg gagtagatat aatttccacc ggttatatca
     2341 cccatcatgc tacctggatt gatatgagtc tgaaaatgga agaaaatgcc tgaccgctac
     2401 gatgtcttaa tcattggtaa tggcattgcc ggaagcagcg ctgctctttt tttggctgaa
     2461 gcagggaaga gcatattact cctcaccaaa ggtgaagatt tagaggacac caacactgct
     2521 aaagctcagg gaggaatagt attccgagga gagggcgata atccttcttt tctcatgaaa
     2581 gatatcatgg aagcctcggc attcacatct tacactgcga gcgctcgatt aatatctcgc
     2641 ctgggcccgc ctttggtgca gaaactatta attgatcgct tacaaattcc ctttgaccga
     2701 aggaatggga aatgggacct cttccgagag ggaaaccact ctttccgccg tatcctccac
     2761 gtcaaggatt acacaggaaa agtcatccag gaatacttga acaaagctgt tttgcacgaa
     2821 ccattgattg aggtgaaaaa aaattccctg gcattagacc tcattgtctc ttctcgtgac
     2881 actccccacc ccgaaagccg ctacttaccg ctggagtgcc tgggagctta tgtgtgggag
     2941 gaagaagcgc aagaagtaat tcccattctt gcttctgcta ccattttagc taccgggggt
     3001 ttgagcgaaa tttaccgcta ttcttcagga ggtccgtgga acacagggga tggctttgct
     3061 atagcccgaa gagcaggagc tcgcctgagc aatatggaat atgtacaatt tcaccccacc
     3121 ctcctttata cccctcaata ttcttcctct tttctcattt ctgaatcagt acggggagaa
     3181 ggagcagagc tcataagcgc aaacggagag ccttttatgt accgatatca cccccgggga
     3241 agtttagctc ctcgagacgt ggtagcgaga gcaatttttc aggaaatgaa aattcacgag
     3301 agtaattgtg tttatttaga cttccggcaa aaaatggagc cccgtaaaat aaaagacact
     3361 ttccccacca tttatgaaga acttctccaa aggggaatag atatcacgca ggaaccggct
     3421 cctgttgctc ctggtgctca ttttgcctgt ggaggagtgc tcaccgatac ccggggaaga
     3481 accaccataa aaagattgta cgccgcagga gaagtagcat gcagcggctt acacggagcc
     3541 aatcgtttag cctctaccag tttgctcgag ggacttacct ttggatatcg agcagctcgg
     3601 gatatagtaa aacatagtga agagcttcct aattttgctg ttcttccttt tcgggagaaa
     3661 tggggagaac ctcccccaga aaacatttta cgcagtctga aagaaatcat ccggataaat
     3721 atgtgggaaa atgtaggact aataagggaa agaagaggtc tttctcaatc tttacagata
     3781 ttgaccgcca tgaaaaaaga aattgctcat ctcaaagaag aatacggtat atccccggcc
     3841 cttttagaac tggaaaacat ggccgaaacc gctcttttag taacggaaga agcttttaaa
     3901 aatcctctat cccggggaac tcattttcgc ctggacgctt ttgatccata aaggggagca
     3961 gacttctcct cttttttctt gttctctcca ttttctcggg ctcttaacct ccactggttt
     4021 acaagatgta cagggttcaa gcccttgagg atttcgtcgt tgtgaagagt caaataaaaa
     4081 gcccaggaag gtttctcctc cagaagaata gacataagca cttgagccaa cttctgggag
     4141 tcatgacggc tgggatgatg ataagaaata aggtcggcct ttataacctt aacagaagaa
     4201 ggtatttctt ccccattacc tcgaaccatc tctgcccccg agagagataa ctcctcctcc
     4261 cccgcttcac tgggtatccc attattcacc aggatatagt tcaagcgctg aggaggaata
     4321 aactggaaga ctgcttgcag atgatcagaa aaatcatagc catccgtttc tccgggttga
     4381 gtggtgatgt tacacacaaa aaccagagga gcaggagaac gcaaaatagc ctccctcact
     4441 tcctccactg ccagattgca caaaacactg gtatatagac ttcctggccc cagaactatc
     4501 aggtctgctt cttcaatggc tttaaccacc tcaggagtag tgtgcaccac tgagggctcc
     4561 agaaagatcc tctttatttt tttcccacag gtgcccacct gagattctcc caggatgatt
     4621 gacccatctt cacattcggc acggaggaag acattctgaa cggtagtagg taaaactttt
     4681 cctctcaccg ccaggatttt gctggattca aaaaccgctt tttgaaaatc tccagtaatt
     4741 tgagccagag cagtgataaa gagattacca aaattatgcc ctcccaggcc ggtatcctcc
     4801 ccaaagcgat actgaaataa ttcactcatc agaggctcga catcagctaa agcaatcaag
     4861 cagttgcgaa tatcaccggg gggcaaaact cccatctctt ctcgcaagcg accggagctc
     4921 cctccgtcat caaaaactgt aaccactgcc gtggtgttgg aagtataatc tttcagtccg
     4981 tggagaagag aatataaccc gtgtcctcct ccaatagcca cgatgcttgg tcctttttcc
     5041 agttgccttt ttctgaaaac ttcattaacc acttcttttt ctggaagaga agaaatggca
     5101 gtaataaccg aacggttcat cttctgcagg ccatagacta cgagcgccac gccccccacc
     5161 aaccatgcca gaccaaaaac tatagccagc cagtaacggt ttaaaaaccc aaagacaaaa
     5221 tttctccaca gcacatatat gcttctaaaa taaggagtgg aaacaatcaa gctcacccct
     5281 aaagagatga gaaacacacc cacaatgacc agaaacaacc atcgctttac ccgcatcccc
     5341 gggtacaacc accgcaaata ttgcttaaaa ggttggctca tcaacctttc ctcctcagac
     5401 accagaacag aattatatat taaaccccta aagcatgttt aactttctca tttaccgagt
     5461 ataattttac cacaaactat aagaggagaa aaaccctcct tataaccctc ttctgtcgcc
     5521 gcaatgaagc tcctcatcca tcaccccggc gactttcgag ccgtttttag cctgcccctg
     5581 aatg
//
LOCUS       DAOPSS010000102         5555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_11915_length_5555_cov_7.076545, whole genome shotgun sequence.
ACCESSION   DAOPSS010000102 DAOPSS010000000
VERSION     DAOPSS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11915_length_5555_cov_7.076545"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            859..1899
                     /locus_tag="PKN31_06430"
     CDS             859..1899
                     /locus_tag="PKN31_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOA99595.1"
                     /translation="MKRLLCLAIILGMVAAGFSAIAQEEPVYLGTAIRSLSNPYHAVW
                     AEGATAFAKSLGWEEYNVIQTCEGSSEKQLNDIKALVARSQGNVVFSIDPNQATDTPA
                     IANELEKSGVYFVTWWNKPDEVKVSDYKYWVCHVTFDNRVSGYNTAKILFDSIGGSGK
                     VFALQGLLGNSSTIERWEGFQKALAEYPEIELVGWQAADWEKTKAYNQVSNALIANPD
                     IKAIWCANDAMAIGALEALRARELIGKVPVSGVDGIPEMITAIKNGEAVATVSWDGFW
                     QGGMGLSIPLAVKQGKIKIEELPENKREWIAESILITQENIDWYIENYVEGTPEYDWN
                     DYWGKWVRGIRE"
     gene            1974..2963
                     /locus_tag="PKN31_06435"
     CDS             1974..2963
                     /locus_tag="PKN31_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99596.1"
                     /translation="MESKTAVLSDSEKTWLSGIGANPSLLALIILVIFFSLLNPRFFT
                     LSNLFNILDLASLLVITSLSLAFILMMGSIDLSVEGVVGLCSVVASLLVKNFSNSNDF
                     GFLALIICGLVGGGFGFLNGFILSRFKIPSFMVTLGIGYITTGLGVLITRGNPVVITD
                     WSFRLLSLGKVGFLPFPFILALGLFFVIWFISERTAFGRYVLALGGDETIVQDLGVPT
                     SKIKTKAFLIGGILYGIVGALLTARLGSGDITAPLGFTFDSICACVLGGIAITGGVGN
                     IPRAIIGAFILTILRNGMVLMGISPYVQEGIIGVVLIATVALTIDREKIKIMK"
     gene            3005..4516
                     /locus_tag="PKN31_06440"
     CDS             3005..4516
                     /locus_tag="PKN31_06440"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA99597.1"
                     /translation="MEPVLRMENISKTFPGVRALEDVNIEVFPNEIVGLVGENGAGKS
                     TLVKILAGVYRADKGEIFLRGQKVGIKNPNDARALGIGMVFQEQAVLPNMRVYENLFL
                     GREKFFVRRGTLDQKLMMQEATKILEEVGLELSPQVYVHELNFMERQMVEIARNIWLA
                     RQARVDYPIIILDEPTTVLEEEEIERLFNKLRELKKRVSFIYISHRLKEVVELCDRVY
                     VLKDGQNAGCFFHEEVSEELLRSRMVGRELHEEYYLASKQREPAEKVVLELRNCTKRD
                     AFYDISFRLHQGEILSLCGTVGSGKEELCRALYGEVKLDSGEIWMERERVNINSPDQA
                     FALGIGYISEDRRNEGLILHLPIFENITLPIVHRLVWGFLVKRKEQFRLSQEMINKLR
                     IKAPSPSVLCFNLSGGNQQKVVIAKWLLSQVKVLIMSHPTRGVDVGAKHEIYALIREM
                     AENGMAMIVMGDSFEEDIGLANRIIVMKDGRISGVLDATESKPAPSDLIQYVV"
     gene            4557..5489
                     /locus_tag="PKN31_06445"
     CDS             4557..5489
                     /locus_tag="PKN31_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99598.1"
                     /translation="MDKGKLLALSVVAVLFAVLAFSSPQAFLSRYNIGTLLDYAATYF
                     IAAIGLTFVIMIGSIDLSVGGMLSLFTVVFVLALNYFGFWAYFITIGLGFLLGMANGL
                     VFTKLKIPSFIGTFGTSGVFASLALIVSGGRPIGLRPQALSLLNPLTFELGPIKGSHL
                     LAFVLFGIFFLIQNSTPFGKYVAAIGHSERATLLSGIKVDRTKIFCFALSGLSAALAS
                     IVLVSRMLSGDPTIGTPYQLQVIAAVVVGGTALTGGVGGVFNTLLGTLIITLVGNGLY
                     VVGIDVYYQMIITGIITMVAVILTLDRTKIKVVK"
BASE COUNT         1566 a          817 c         1327 g         1845 t
ORIGIN      
        1 cagaaagaga cgaaggaaaa agttaaagag ctgataactt cctactatga aatagagggc
       61 tcggtacata ttctttctaa aaaactataa aagggatata tgaggttacc ataatggact
      121 ccgaaaactg ggcagagcaa taagactgta agatcattca gtgaatatgg gacgcatcct
      181 cgcaagataa cgaaatggaa gaaacaacta cttgaagaat taccttcaat atttagtgat
      241 aaaagagtaa agacaaatgg agagttaaaa gtaaagtaag aatggctcaa aaaaactatc
      301 ctcttcgggg aggaaaagca taaagtgaca gagccaatga atccggagat actattatat
      361 ggacaatgtg agcttatagg gttttcttgc tcttccacac tttttggtct ttcgaaagat
      421 ttagggagga tgctagaagt aaactgaact ctctacttac ttcttttcga cgatctttta
      481 ctcgtggacg gacgccggga ttgagcctaa tccagtaaga atatctctct ggtagataac
      541 cattacgaac aatagctcgg cgtcctccct catcttttgg agtgtggtat tgattgataa
      601 attcctctac ttcagcttcg gagctggctt tacattgact ttcaaagggg gctgtgatag
      661 attgactgtt agtggtaatt accatttaat gtatttgtct aatcgaagga ggtggtaaaa
      721 tataagttaa agctagctag tacaaattag ttttatgtgt aaatggaata atcatttttc
      781 aagcttagtt tttaaatgtg ttaaaggtta ttatttgatt aacttttatg tgtcaagaga
      841 agggaggcaa gttgctttat gaagaggttg ctttgtttag ctattatatt aggaatggtc
      901 gccgcaggtt ttagtgctat tgctcaggaa gaaccagttt acttgggtac agccatcaga
      961 agtttgtcta acccctacca cgcagtatgg gctgagggag ctactgcttt tgctaaatct
     1021 ctgggttggg aagaatacaa cgtaattcaa acttgtgaag ggtcgagcga gaagcagttg
     1081 aatgatataa aagctctggt ggcccgttcc cagggtaatg tagttttttc tatcgatccc
     1141 aaccaggcta ctgatacacc tgctatcgca aatgagctgg aaaaaagtgg agtttacttt
     1201 gtgacttggt ggaataagcc tgacgaggtc aaagtttctg attacaaata ttgggtatgc
     1261 catgttacct ttgataaccg tgtttctgga tataataccg ccaaaatcct atttgatagc
     1321 attggtggtt caggcaaagt tttcgccttg caaggattgc ttgggaatag ttccacaatt
     1381 gagcgctggg aaggttttca aaaagcattg gctgaatatc cagaaattga attagtgggt
     1441 tggcaggcag ctgattggga aaaaacaaaa gcttataacc aagtttctaa tgctttgata
     1501 gctaatcccg atataaaggc gatatggtgt gccaatgatg ctatggctat tggagcttta
     1561 gaagctttgc gcgcgcgtga attgataggc aaagtaccgg tatccggagt tgatggcatt
     1621 ccagagatga taaccgccat taaaaacgga gaggcagttg ctactgtatc gtgggatggt
     1681 ttctggcagg gaggaatggg actatctatt cctttggcag ttaaacaggg taaaattaaa
     1741 atcgaagaat taccagaaaa caaaagagag tggattgctg agagtatttt gattactcaa
     1801 gaaaatatcg attggtatat tgagaattat gtggaaggta ctccagaata tgattggaat
     1861 gactactggg gaaaatgggt gcgaggtata agagaatagt aataacttta taattttatt
     1921 tagcccggag aagattctcc gggctaaatt tcttaagggg gaataaaaga gacgtggaaa
     1981 gcaaaacggc tgttttatct gatagtgaaa aaacttggtt gagtggaata ggagcaaatc
     2041 cttccttact ggctttaatc attttagtta tattttttag tttattaaat cctcgtttct
     2101 tcactctttc taatttgttt aatattctgg atttggcttc tttacttgtg ataacttcgc
     2161 tttctctggc ctttattctt atgatgggga gtatcgatct ctcggtggaa ggagtagtgg
     2221 gtctgtgtag cgtagtagca agtttattag taaagaactt ttctaactct aatgattttg
     2281 gttttttggc tttaattatt tgcgggttag tgggaggtgg ttttggtttt ttgaacggtt
     2341 tcatactttc tcgctttaaa attccttctt ttatggttac tttgggtatt ggctacatta
     2401 ccactggtct tggagtttta atcactcggg gtaacccggt agtgattacc gattggagct
     2461 ttcgtctatt aagtcttggt aaggttggat ttttaccttt tccttttatt ttggctctcg
     2521 gtttattctt tgtaatctgg tttattagtg aaaggacggc ttttggtcgg tatgttttgg
     2581 ctttgggagg agatgagact attgttcaag acctgggggt acctacttct aaaattaaaa
     2641 ccaaagcttt tttaattgga ggaattctat atgggatagt cggggctctg cttacagcgc
     2701 gcttgggctc aggagatatt actgctcctt tgggttttac ttttgattct atatgtgcat
     2761 gcgttttggg aggtattgct attactggtg gagtaggaaa tattcctcga gctattattg
     2821 gagccttcat tctaactatt ttgcgcaatg gaatggtttt gatggggata agcccgtatg
     2881 ttcaagaggg gattattgga gtggtattga ttgctacagt cgctttgact attgataggg
     2941 aaaagataaa aataatgaaa tgactttgaa ttttggttaa ataagcattg ggaggttcaa
     3001 acttatggag ccagtcttga gaatggagaa tatttctaaa actttccctg gagttcgagc
     3061 cctggaggat gtaaatatag aagtttttcc taatgaaatt gttggtttag tgggagaaaa
     3121 cggagcggga aaaagtactt tagtgaaaat tttggccggt gtttataggg ctgataaagg
     3181 agaaatcttt cttcgcgggc aaaaggtggg gattaaaaat cctaatgatg ctcgagcgtt
     3241 gggaattggg atggtttttc aagaacaagc agtgttgccc aatatgcgag tttatgagaa
     3301 ccttttttta gggcgagaga agttttttgt tcgtagggga actttagacc aaaaactaat
     3361 gatgcaggaa gcaacaaaaa ttcttgagga agtggggtta gaattatctc ctcaggttta
     3421 tgtccatgaa ctaaatttta tggagcggca aatggtagaa attgctcgaa acatctggct
     3481 agctcgtcag gctcgagttg attaccccat tattattctg gacgaaccca ctactgtttt
     3541 ggaagaagaa gaaattgaaa ggctgtttaa taaactcagg gagttgaaga agagagtttc
     3601 tttcatttat atttctcatc gattgaaaga agtagttgaa ctttgtgacc gagtttatgt
     3661 tctaaaagat ggtcaaaatg ctggatgttt ctttcatgag gaagtttctg aagagctttt
     3721 gcgctctaga atggtaggac gagaattaca tgaggagtat tatttagcat caaaacaaag
     3781 ggaaccagca gaaaaggttg ttttggaact aagaaactgt actaaaagag atgcttttta
     3841 tgatatttct tttaggttgc accagggtga aatactgagc ttgtgtggaa cggtaggttc
     3901 ggggaaggaa gagctttgcc gagcccttta tggtgaagtc aaacttgatt ctggagaaat
     3961 ttggatggaa agggaaaggg taaacattaa ttctcctgac caggctttcg ctttaggtat
     4021 tggatatatt tcagaagacc gacgaaatga aggactgatt ttacatcttc ctatatttga
     4081 aaatattaca ttgcctattg ttcatcgttt agtttggggt tttttagtca agcgaaaaga
     4141 acaatttcgt ctttcacagg aaatgataaa taagctacgg attaaagctc cttctccatc
     4201 ggttttgtgt tttaacttaa gtggaggtaa tcaacagaaa gtagtaatag ctaaatggct
     4261 gctttctcaa gttaaggttc tcattatgtc tcatcctact cgaggagtgg atgtaggggc
     4321 aaaacacgaa atttacgctc tcatcagaga aatggcggaa aatggaatgg ctatgattgt
     4381 catgggagat agctttgaag aagatatcgg tttggccaat cggattatag tcatgaaaga
     4441 tgggagaata agcggtgtgt tggatgccac cgagtctaaa cctgctcctt ctgatttaat
     4501 acaatatgta gtgtagtttg agtgattttc aggataaatt tataaggagg acgaaagtgg
     4561 ataaagggaa attgttagct ctaagtgtag ttgccgttct ttttgctgtt ttggcttttt
     4621 ctagtcctca agctttcctc tctagatata atattggaac tcttctggat tatgctgcta
     4681 cttattttat agcagccatc ggtttaactt ttgtaattat gattgggagc atagacttat
     4741 ctgtaggagg gatgctctct ctttttactg ttgtttttgt tttagctctt aattattttg
     4801 gtttttgggc atattttatt accattggtt tgggtttcct tttgggaatg gccaatggct
     4861 tagtttttac caaacttaaa attccttcct ttattggaac ttttggaact tccggggtat
     4921 ttgctagcct agccttaata gtttctggtg gtcgtcccat tgggttgcgt cctcaagctt
     4981 tatctcttct taatcctttg acttttgagt taggaccaat taagggttca catttattgg
     5041 cttttgttct ttttggtata ttttttctga ttcaaaattc tactcctttt ggtaaatatg
     5101 tagcggctat cgggcattca gaaagagcta ctttgttaag tgggattaaa gtagaccgta
     5161 ctaaaatttt ctgttttgct ctatctggac tttcggcagc tttagcctcc atagttttag
     5221 tttctcgaat gctgagtgga gaccccacta ttggaacacc ttatcagctt caggtaattg
     5281 ctgcagtggt agtagggggt actgctttga ctggtggagt agggggtgta tttaacaccc
     5341 tgcttgggac tttaatcatt actttggtgg gtaatggttt gtatgtagtg gggatagatg
     5401 tatattatca gatgataata actggtatca ttactatggt ggcagttatt ttaactttag
     5461 atcggactaa aataaaagta gtaaaatgat tgaaaacaag ggaggtttta ctcttggatt
     5521 taggtctcaa gaataagata gcagtaataa ccgga
//
LOCUS       DAOPSS010000103         5492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12091_length_5492_cov_8.823984, whole genome shotgun sequence.
ACCESSION   DAOPSS010000103 DAOPSS010000000
VERSION     DAOPSS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12091_length_5492_cov_8.823984"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            617..1489
                     /locus_tag="PKN31_06450"
     CDS             617..1489
                     /locus_tag="PKN31_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOA99599.1"
                     /translation="MDITPEVHAQLLEHQKNEITEYHIYLNLSKNPKSSKNQEILEKI
                     AHEELRHYHVFKKYTRKDVEPDRFKIWKYYWISRIFGLTFGLKLMENGEERAQKNYEH
                     LLETIPEISTIIEEENKHENALLGLIDEEWLNYIGSVVLGLNDALVELTGALAGFTLA
                     LQDTKLIALTGSVTGIAAALSMGASEYLSTKAEGTDKNPLQASLYTGVAYILTVILLI
                     LPYLLLSNYYLCLGCTLAIAVAIIALFNYYISVAKGTPFGKRFWEMIGVSFGVAGFSF
                     FIGYLMRSVFGIEV"
     gene            complement(1502..3235)
                     /locus_tag="PKN31_06455"
     CDS             complement(1502..3235)
                     /locus_tag="PKN31_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOA99600.1"
                     /translation="MKGFFVDWIEKPAERKEKRDKIIVLLLVTFYLIVSFYHLGSLKA
                     PQTYWQPSSPGEGACLDLGKEETIKRISFFGGLIGEGEYRLEYSADAETWQEGPLLKP
                     QNVFEWKEADVDLRGRYIRIIAQKSGGMLNEIGFWGEKQTLLPVNAILPLHQGYLLPQ
                     GFPEAFDEQDTVPYQSSYLNSTYFDEIYFARTAYEYLHQVEPYEWTHPPLGKMLISLG
                     ITIFGMNPFGWRFMGVVFGALIIPLMYLLGKKLFGRSEYGLVAAFLMTFEFMHFVQAR
                     IATIDTYVVFFIILMYYFMLVYLSIPYSPSETRRFLLPLFLSGLSFGLGASVKWTGIY
                     AGGGLAVLFFLDLVKKRKENPSSFATFCKKMFPWCVLFFIIVPLFVYCLFYVFFLPGP
                     TGIRDIWRHQLQMFNYHSKLEATHPFSSPWWQWPLMIRPIWLYQGKGLPPGQISSIVS
                     LGNPAIWWGGTLVLLFSLILPLFRKEKALPFILIGFLAQYLPWVLVPRLTFIYHFYNS
                     VPFYILLIVLFYRKIRENYPRYKPFLFGYLALAAFLFFLFYPVLSGEIVSKNYVATYL
                     RWLPSWTFFIN"
     gene            3777..5279
                     /locus_tag="PKN31_06460"
     CDS             3777..5279
                     /locus_tag="PKN31_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOA99601.1"
                     /translation="MKRVVIIVVLLVAAILAGYTEEVSGKQSPEELKYDLDFLIQTLE
                     DVHPDLYRYTPQEEIYKMKQDIEEDLSEPLTRLEFYRKVAPLVTRIGDGHTYITYPVE
                     ELNEYINGGGRLFPLDVRIIGERLFVVVDYTGEIPPGAEILKINGFSISQLIEELTKY
                     VSGEKLSYRIESVNTNFRALLWFAYSWEEFNITYLSPSGKALQKPIKGMTWNELTRKA
                     QELQKEFAKEEKEEDSKKIQYPYYSYASMPELKTGVIDFRAFQDPEKFNLFLEETFRK
                     IKEEEIEHLIIDIRNNSGGNSTLGDALLDYLTDKPWRQFSKIELKVSKQIKEYYKDPL
                     PALKKIYPESYESMYQASQEWYHSVYQEISSLPEGEVFSSSPELIEPSENPLRFHGKL
                     YVLIGSHTFSSAVNFAATVKDYGIGTLIGEETGGLPTHFGDIYPFWLPNTDLPAGVSH
                     KKFYRPSGIDDGRGVLPDIEVTTTLEDVLEGKDPVMEHILEVIKTIELVP"
BASE COUNT         1795 a         1092 c         1118 g         1487 t
ORIGIN      
        1 gatcttactt tgaattgcgt atacacagcc atcacaacaa ttaggtagaa aaagaagaga
       61 gagaatgagt atattgtttt aaaattttcc aaaatttttt taactaccta ttgacaaatt
      121 caaaaagaga tgatatatta tgtttgccat cggttctgag gagttaagcc ggaggatgga
      181 aaaaagagct tctgtttgaa agataagaag ctcaaagctt ttgagtggaa gtccatcctg
      241 aagtttctca ccccaaaaag gtgagaaaga aaaggctaag gtttgttaga accgatggct
      301 tttttattcg gccttataac cacagaacca cagtaagaca cttcccctat aacctgacgc
      361 cctttttccc cctctttaca aagagcaagg ccgtcttttt ttagagacag agctaccaca
      421 caggtgaaaa agtgaggtta attatgacaa cccaacggcg caaggttatg gagagaaaga
      481 cttctccaca gctctcctta agcaatcttt tatcattggc ttcccctctc tttaatcctt
      541 ctcattgttt ttatacctca tcaaggttaa aatagacgca gcaaccttga atacctgaga
      601 gtaggaggga gaaaaaatgg atatcactcc tgaagttcat gctcagttat tagaacacca
      661 gaagaatgaa attaccgaat atcacatcta tctaaacctg tctaaaaacc ccaaatcctc
      721 gaaaaaccag gagattctgg agaaaattgc ccatgaagag ctccgtcact accatgtttt
      781 taaaaagtat acccggaaag atgtagagcc agaccgattc aaaatctgga aatattactg
      841 gataagtcgt atttttgggc tcactttcgg gttgaagcta atggaaaatg gggaagaaag
      901 agcacaaaaa aactatgaac atctactgga aactattccc gaaataagca ctatcatcga
      961 ggaagaaaat aagcacgaaa atgccctgtt agggctcata gatgaggaat ggcttaacta
     1021 tatcggttcg gtagtgttgg ggctcaacga tgctttagtg gagctcactg gtgctctggc
     1081 tggttttact ctggctctcc aggataccaa attaatcgcc ctgactggtt cggttaccgg
     1141 tattgcagca gctctatcta tgggagcttc tgaatattta tccacaaaag cagaagggac
     1201 ggataaaaac cccctccagg cttctcttta tactggggtt gcttatattt tgaccgttat
     1261 tctacttatt ctaccctatc ttctgctaag caattactat ctttgcctgg gttgcacttt
     1321 agctatcgct gtcgccatca tcgctctctt caattactat atttcagtgg ctaaaggaac
     1381 tccttttggg aagcgtttct gggaaatgat aggggtaagc tttggggtag ctggctttag
     1441 ttttttcatt ggttatctga tgcgctctgt ttttggtata gaagtttgaa tgactataat
     1501 tttagttgat gaaaaatgtc cagctgggga gccagcgcaa gtaagtagct acataatttt
     1561 tgctcactat ttctccagag agcacaggat aaaaaaggaa gaataaaaag gcagcaaggg
     1621 cgagataccc aaaaagaaaa ggtttatagc ggggataatt ttcccggatt ttccgataaa
     1681 aaagaacgat caaaagaata taaaaaggta cactgttata aaaatgataa ataaaagtca
     1741 atcgagggac caaaacccag ggaagatatt gagcaagaaa tccgattaaa atgaaaggaa
     1801 gagctttttc ttttcgaaag aggggaagga taagagaaaa gagaagaaca agagtacctc
     1861 cccaccagat agccgggtta cccagagaaa cgatgctcga gatttgtcca gggggcaaac
     1921 ccttcccctg gtaaagccag ataggtcgaa tcataagagg ccactgccac caaggagaag
     1981 aaaaaggatg agtagcttca agtttactgt gatagttgaa catctggagc tgatgcctcc
     2041 agatatctct tatccccgta ggtccgggta aaaagaatac gtaaaataaa caatagacaa
     2101 aaagaggaac gatgataaaa aataaaacac accagggaaa cattttttta cagaaagtag
     2161 caaagctcga aggattttct tttctcttct ttaccagatc gagaaaaaag agcaccgcca
     2221 gccctccccc ggcatagata cccgtccatt ttactgacgc tcctaacccg aaggaaagac
     2281 cagagagaaa aagaggtaac aaaaatcttc tggtttccga aggagagtaa ggaatggaga
     2341 gataaacaag cataaagtaa tacataagga tgataaaaaa taccacatag gtgtctatag
     2401 tagctatacg agcctggaca aaatgcataa actcaaaagt cataagaaaa gcagccacta
     2461 acccatattc acttctgcca aataactttt tccccagaag gtacatcaaa ggaataatca
     2521 aagctccaaa caccactccc ataaatctcc acccgaaggg gttcatacca aaaatagtta
     2581 tccccaaaga gattaacatc ttgcccaggg gaggatgagt ccactcataa ggttctacct
     2641 gatggaggta ttcataagca gtacgggcaa agtaaatctc atcaaagtaa gtgctattta
     2701 gataagagga ttggtaagga acggtatctt gctcatcaaa agcttcagga aaaccctggg
     2761 gaagtaggta tccctggtgt agaggtaaaa tagcatttac aggaagcagg gtctgttttt
     2821 ctccccaaaa accaatttcg ttgagcatcc ctccactttt ttgagctata attctaatat
     2881 atcttcctcg caagtcaacg tccgcttctt tccactcaaa aacgttctgg ggcttaagaa
     2941 gaggtccttc ttgccaggtt tcggcatcag cggaatattc aagccgatat tctccctccc
     3001 cgataagccc cccgaaaaac gatatacgct tgatagtttc ttcctttcct aaatcgagac
     3061 aggctccctc tcccggagaa gaaggttgcc aataggtttg aggagcttta agagaaccta
     3121 aatgataaaa agagacgata agatagaagg taaccaataa caaaacgata attttatctc
     3181 ttttttcttt tctttcagct ggtttttcta tccaatccac gaaaaatcct ttcatttctg
     3241 gcatattttt actacttaag aattatataa cattccgtct gtttgcaatg ctaagaatta
     3301 ccttatttaa aaatcaaagt ggggagaatc atccccataa actgctgaaa gtgctgagtg
     3361 tagtggcaaa atagcatatt ctaatttaaa gaatatagat ttgacgcggg tttcaactaa
     3421 atgaagtgtc aaagtcagag gttataagag tggttcaaag agacgttttc ctctccagcg
     3481 gtgtccactt tttccgggga ttatataaga ggctctcctt cctatagtcc cttctcgtta
     3541 ttcccgaaat ctttagacgg gaatccactg aggaacgctt ttcccctcag tagttttatc
     3601 tttttattca agtttttagt tttagtcttt tattttagtc tctcggggat tataaagggc
     3661 gaccctcata gtcattgcca tgactgattt tatgggatac tataatagcg aattatagtt
     3721 agagacaata agaaaaccaa tggaaaggaa caaaaattga taaaggtgga aagaaaatga
     3781 aaagagtcgt aataatcgtt gtgttgctgg tagcggcaat tttagctggt tacacagagg
     3841 aggtttcagg aaagcaatca ccagaggaat taaaatacga ccttgatttt ctcatccaga
     3901 cactggaaga tgtccacccc gatttataca gatacactcc tcaagaggaa atttataaga
     3961 tgaagcaaga tatcgaggaa gacttgagtg aaccactgac caggcttgaa ttttacagaa
     4021 aggttgcacc gctggtaacc agaattggag atggtcatac ctacattacc tatccggttg
     4081 aggaattgaa tgaatatata aatggagggg gacgcctttt cccgcttgat gtcagaatca
     4141 ttggcgaaag actattcgtc gtagttgact ataccggaga gattccacca ggtgcagaaa
     4201 tactcaaaat taatggattc tcaatttcac agctcattga ggaactcact aaatacgtga
     4261 gcggagagaa attatcgtac cggatagaat cggttaacac aaactttcgg gccctgttat
     4321 ggtttgcata tagttgggaa gagtttaaca taacgtattt atctccctct ggcaaagctc
     4381 tccagaaacc tataaaaggg atgacctgga acgagctcac cagaaaagct caggagcttc
     4441 aaaaagaatt tgctaaagaa gaaaaagagg aagacagtaa aaaaattcaa tatccctatt
     4501 acagttatgc ttcaatgcct gagttaaaaa caggagtaat tgattttaga gcctttcaag
     4561 atccggaaaa gtttaattta ttcttagaag aaacattcag aaaaataaag gaggaagaaa
     4621 ttgagcacct tattatagat attcgcaaca acagtggagg aaactctact ctgggtgatg
     4681 ctctcctcga ttacctgaca gataaaccct ggaggcaatt ttcaaaaatt gaacttaaag
     4741 taagtaagca aataaaggaa tattacaaag acccccttcc agcattaaaa aagatttatc
     4801 cagaatccta tgaatcgatg tatcaagcat cccaggaatg gtatcactca gtgtatcagg
     4861 aaatctcttc tttgcctgaa ggcgaagtat tttcttcttc tccagagctc atagaaccct
     4921 ctgaaaatcc ccttcgattt catggcaagc tatatgtatt aataggctcc cacacctttt
     4981 ccagtgccgt caattttgca gccacagtta aagactacgg catcggcaca cttattggag
     5041 aagaaacagg agggctgcct actcattttg gcgatattta tcccttctgg ctacccaata
     5101 ccgaccttcc agcaggggtt tcacacaaga agttctatag acccagtgga atagatgacg
     5161 gaagaggagt tttaccagat atcgaagtta ccaccaccct tgaggatgtt ttagaaggca
     5221 aagacccggt gatggagcat attctggagg ttataaagac tattgagctt gtgccctgat
     5281 ttttatcctg cagaacctgc tataattatt ctcttttccc gacgtctccg tgagacatta
     5341 ttttcgtatt ccgtggcggt ttctattttt tgatccctta cggagtagaa aaaacgagac
     5401 tgccacgacg ctgaaacacc ttttcccggt ataattattt tctcgtttat ttgcttttaa
     5461 agctcacact gtaggcgtct cgctcgcaaa ga
//
LOCUS       DAOPSS010000104         5477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12132_length_5477_cov_8.415345, whole genome shotgun sequence.
ACCESSION   DAOPSS010000104 DAOPSS010000000
VERSION     DAOPSS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12132_length_5477_cov_8.415345"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..886)
                     /locus_tag="PKN31_06465"
     CDS             complement(281..886)
                     /locus_tag="PKN31_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOA99602.1"
                     /translation="MKRKFDRVYQFKITLKRIKPPIWRRIQVPETYTFWDLHVAIQDV
                     MGWQDYHLHRFEIVNPATGALKLIGLPSDDDDWSRATLSGWRQKIAKWFTPENKVAVY
                     FYDFGDGWEHEVELEKILPRETGVRYPVCLGGKRACPPEDCGGVSGYERLLEIISNPD
                     DDEYEEMLTWLGGKFDPEHFDPKKVVFDDPAIRFKKAFEEE"
     gene            complement(924..2294)
                     /locus_tag="PKN31_06470"
     CDS             complement(924..2294)
                     /locus_tag="PKN31_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99603.1"
                     /translation="MTPVNPREQLIGLGAEKLADALLDLSTRSDQARKLVERLLSSPE
                     DSLRYFKAKLAGLKRRERFVGKGEARSYAQELADFLADIQAVIEDPKTGLELVVALFE
                     IDSKILESCDDSYGFMGEIFRFDATELFTHYARDIANKSYLSDLVFKLYQEDEYGVRE
                     KLVDQASQFLPEETLRFLAQRFWENAENIDKTTTDGQCAARHSLFAVESLARQLQDAP
                     LFEKAALAIWPDLSPATCLNIAEVYLEAQDPGKALHWVKQIPQKTILEEYRRDKLLLD
                     VYRKINDQEKLAEVAWKMFRQHKDIDTLEELLSIIGEHQREKVIAEASQEILVTPSFS
                     YSDLSFLLDTNQIDLAQKYLLENVTALNGDLYNLMLPLAQRFEKENRLLAATVIYREL
                     LESILSRAQSKYYHHGVRYLKKLEELAPQVSDWQGFPPHELYYQKIAETHARKKSFWL
                     KYEKGK"
     gene            complement(2427..2672)
                     /locus_tag="PKN31_06475"
     CDS             complement(2427..2672)
                     /locus_tag="PKN31_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99604.1"
                     /translation="MDGQLFIQFIVLIYLSALRKKMRDTGLIEKYTVWDLLLEWKALT
                     QVCYSSKYGPILTEITKPQTIESLGIDPKHSYNFPGN"
     gene            complement(2791..2961)
                     /locus_tag="PKN31_06480"
     CDS             complement(2791..2961)
                     /locus_tag="PKN31_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99605.1"
                     /translation="MSKTTCGAKKLKDRCKKEITKDKKLLGIENILSACSVGLKVKSN
                     LISREYFITMNL"
     gene            3098..4342
                     /locus_tag="PKN31_06485"
     CDS             3098..4342
                     /locus_tag="PKN31_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOA99606.1"
                     /translation="MNKFYQELFSLFLESPKIDTHEHLLPPKQFRENPDILLSILERS
                     YLSWILSGTTGKSSGLTREELLSRLKTIPASSFYRYLIRAFQFMYEFRENTLNRENWE
                     GLSQQIRESYEKDDMIKKWLSGRLNIQKIILDRYWIVGDFQVDREIFSPVLRLDPFFF
                     GYSREARDHDGQSPYFYADREGIQVETFSEYLSLIDHILQKGMREGISALKLAIAYDR
                     GLSFKQVTRTKAEVAFYRKAGEETPEEIANFQDFIFHYSFQKAEELGLPIQIHTGPGK
                     AFQTAPSLLGPILEEYRTVKVSLFHGGFPWVGEPGAMALFYPHLYLDLVWLPLLSPAF
                     SRLALREWLETTGGARIMMGGDSWNVEGAVGSILFNLETMAQVLGEMVDTGYLSMNEA
                     QEIGKMILWDNPREFFLPPQEK"
     gene            complement(4491..4844)
                     /locus_tag="PKN31_06490"
     CDS             complement(4491..4844)
                     /locus_tag="PKN31_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99607.1"
                     /translation="MPAKREHLRASRILLGTQTPVVHELLELAVKRQGMEHGYLISNS
                     DYLGMIGQLLGQEAKREAVLHILQDWGIVTSEDVDHFSSFIPSRRKSTVSPSALEQKK
                     KHPKKNKTKEKGKNS"
     gene            complement(4810..5085)
                     /locus_tag="PKN31_06495"
     CDS             complement(4810..5085)
                     /locus_tag="PKN31_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99608.1"
                     /translation="MDIKDYTREMADRRFATLETLKTITEWLNATKPTAFLEKGAPVG
                     DDQVREIWRRWVDAYSDLFEKTKTIPDLLIEDVKRLYACEKGTSESQ"
BASE COUNT         1469 a         1186 c         1151 g         1671 t
ORIGIN      
        1 cctgaatggt tctatcagga atccaaatta tttaagggta aaagtctctg gattcccgat
       61 tactaatttc aggactaaca ggggaggccc ccatgctgct tcgcagcacc agataacaat
      121 gaaaacctgg gggctattct gttagtgttt agttttttaa gcttttaagt tttagtcttt
      181 tatttttagt ttttctcctt cgggtggcaa taaggggcac tcccccttat tgccgttcac
      241 aaaacaatca ggcgcgcccc ctatcatctc cttggcgtgc ctattcctcc tcaaaggctt
      301 tcttaaagcg gatggcaggg tcgtcaaata ctactttctt cgggtcaaag tgttcggggt
      361 cgaattttcc tcccaaccac gttaacattt cttcgtattc atcatcgtct ggattgctaa
      421 tgatttctaa gagtctttcg tatcccgaaa cccctccaca atcttccgga gggcaggctc
      481 ttttccctcc cagacatact ggatacctaa ccccggtttc tcgaggtaga attttttcta
      541 gttctacttc gtgttcccag ccatcaccaa aatcataaaa atatactgct actttatttt
      601 ccggagtaaa ccacttggca attttctgcc tccagccgga aagagttgcc ctgctccaat
      661 cgtcgtcatc gctaggaagt cctattagct tcagagctcc tgtagcggga ttaactattt
      721 caaaccgatg aaggtggtaa tcttgccagc ccataacatc ctggatggcc acatgtaaat
      781 cccagaaagt ataggtttct ggaacctgga ttcgccgcca gatgggtggt ttgattcttt
      841 ttaaagtgat tttgaactgg tatacccgat caaattttct tttcatgatt taccccctct
      901 taagattagt gcaaaactta actttattta cctttctcat atttgagcca gaagcttttc
      961 ttccgagcgt gagtctctgc tattttttgg taatacagct cgtgaggtgg aaacccctgc
     1021 cagtcagaaa cttgaggggc aagttcctcc aattttttca aatagcgtac tccatggtga
     1081 taatatttag attgggctcg gctcagaata gattccaaaa gctcccggta gataacggta
     1141 gccgcaagta gccggttttc cttttcaaaa cgctgggcca gaggcaacat gagattatac
     1201 aggtcgccgt ttaaagcagt tacattttcc aatagatact tttgggcgag atcaatctgg
     1261 ttggtatcaa gcaagaaaga tagatcagaa taggaaaaag aaggagtgac caaaatttcc
     1321 tggctggctt cggctatcac tttttctcgt tggtgttcgc caatgatgga caaaagctct
     1381 tctaaagtat cgatgtcttt gtgttgacgg aacattttcc aggctacttc tgctaatttt
     1441 tcctggtcgt tgatttttcg ataaacatct aacaacaatt tatctcgcct atattcttct
     1501 aaaattgtct tttgtggaat ttgctttacc caatgaaggg ctttccccgg gtcttgtgct
     1561 tctaaataga cctcggcaat atttaaacag gtggcaggag aaagatcggg ccagatagct
     1621 aaggcagctt tctcaaacag gggagcatcc tgtagctgac gagctaatga ctctactgca
     1681 aaaagagagt gtcgagcagc acattgacca tctgtggtag ttttatcaat attttcggca
     1741 ttctcccaga accgttgggc caggaagcgc aaagtttcct cgggcaaaaa ttgggaagct
     1801 tggtccacca atttttcccg gactccgtat tcgtcttctt ggtaaagctt gaaaactaag
     1861 tcggacaggt atgatttatt tgctatatcc cgagcataat gggtaaaaag ctcagtggca
     1921 tcgaaacgaa atatctctcc cataaacccg taagaatcgt cacaggattc taaaattttg
     1981 ctgtcaattt caaagagagc taccacaagc tctagcccag ttttggggtc ttctattacc
     2041 gcctggatat cggctaaaaa atctgctagt tcttgggcat aactgcgagc ttctcctttt
     2101 cccacgaatc gctctctccg ctttaggcca gctaattttg ccttaaaata ccggaggcta
     2161 tcctccggag aggatagtaa tcgctcaact agtttcctgg cctggtcgct ccgggtggat
     2221 aaatccaaaa gagcatcggc taacttttca gctcccaatc cgatcagttg ttctctgggg
     2281 ttgacaggtg tcatcgaatt aaacctcacc ctaataactc aagttgattt tcattttacc
     2341 actttttgac ccttgggtta cttttttcag agttggctaa gttcccactg gagccggttc
     2401 catccgcaac ttctaagctt tacatcctaa tttcccggaa aattataact atgtttcggg
     2461 tcaattccca gactctctat agtttggggc ttggtgatct ctgtgaggat gggtccatac
     2521 ttgctggaat agcaaacttg agtcagtgcc ttccactcca ggagcagatc ccaaactgtg
     2581 tacttctcaa tcagcccggt atctcgcatc tttttccgca aagcactcag gtaaatgaga
     2641 acaataaact ggataaaaag ctgaccatcc ataatgggaa aactgtgaat gcggagtcgt
     2701 taaaaccctg ttttcataat tataatcatg actgttatta aaaggatttg tgaagaaaat
     2761 attgccacaa agcagtttat tttcaggttt ttagaggttc atagttataa aatactcgcg
     2821 ggaaatcagg ttacttttaa cctttaatcc tacgctgcag gcactgagga tgttttctat
     2881 ccccagtagt ttcttgtctt ttgttatttc ttttttgcat ctgtctttta actttttagc
     2941 accgcaggta gttttggaca tgacaaaaag ggatgtcacc cctgaatgtc tttacgaggt
     3001 gtccagaggc ttttataaaa agattgcaga gtgaggttac cttctgtggt ttttgtgatt
     3061 aactatataa ttatgataac cgagaggagg tcgaaaaatg aacaaatttt atcaagagct
     3121 gttttctctt tttttggaga gtcctaaaat tgatacccat gagcacttgc ttcccccgaa
     3181 gcagtttaga gaaaaccccg atattctatt atctattctg gaacggagct atttatcctg
     3241 gattctatct ggtaccacag gaaaaagctc tggcttaact cgagaagagt tattgtctcg
     3301 acttaaaacc attcccgcaa gctcttttta ccgctattta attcgagcgt tccagtttat
     3361 gtatgaattt cgagaaaaca ccctcaaccg ggaaaactgg gaaggtcttt ctcagcaaat
     3421 cagagagagc tatgaaaagg atgatatgat aaaaaagtgg ttgtctggcc gattaaatat
     3481 ccagaaaatc attctcgatc gttactggat agtgggagat tttcaagtag atagagaaat
     3541 tttttctccc gttctgcgac tggatccttt cttttttggc tatagtcggg aagcccgtga
     3601 ccacgacggt caaagcccct atttttatgc cgaccgagaa gggatacaag tggaaacttt
     3661 tagcgaatat ttaagcctga ttgaccacat tcttcaaaaa gggatgagag agggaatttc
     3721 tgcacttaaa cttgccattg cctatgaccg gggactttct tttaaacagg taactcgaac
     3781 gaaagctgag gtggcttttt accgaaaagc aggagaagaa accccggagg aaattgctaa
     3841 ttttcaggat tttattttcc attactcttt ccaaaaagct gaggagttag gccttcccat
     3901 tcaaattcac accggcccgg gcaaagcttt tcagacagcc ccctctcttt tggggccaat
     3961 tctggaagaa taccggacag taaaggtttc tcttttccat ggagggtttc cctgggtggg
     4021 agagccagga gccatggcgc ttttttatcc ccatctctat ttagatttag tatggcttcc
     4081 tcttttatcc cctgcttttt ctcggctggc tctcagggag tggctggaga ctaccggagg
     4141 agcccggata atgatggggg gagattcctg gaatgtagaa ggagcagtgg ggtcgatatt
     4201 gtttaatctg gagactatgg ctcaggttct gggagaaatg gtagatactg gatatttaag
     4261 catgaatgag gctcaggaaa tcggtaaaat gattctctgg gataatcctc gggagttttt
     4321 cctcccacct caggagaaat aagcaattta tgatgacaga agggaggaga tacgctgaag
     4381 aaacggccat aaaaagagac tctgtttgcc ctggttaata aataaaatac agggagtatg
     4441 cttaaccaca aaaactctga taatactacc gaggaaaatt cctatctctt tcaggagttt
     4501 ttgccctttt cttttgtttt attctttttt gggtgttttt tcttttgctc aagggctgaa
     4561 ggagagacag tgctttttcg ccgagaagga ataaaagagg agaaatgatc cacatcttcc
     4621 gaggtaacta tcccccaatc ttgcaaaata tgtaaaacag cctctctttt ggcttcttgt
     4681 ccgaggagct ggcctatcat acctaaataa tctgagttag aaatcagata accgtgttcc
     4741 attccctgcc gtttcactgc cagctccaag agctcatgca ctaccggggt ttgagttccc
     4801 agcaaaatcc tactggctct cagatgttcc cttttcgcag gcataaagtc tttttacatc
     4861 ctcaatcagt aaatcgggta tggttttagt cttttcaaat agatcactat aggcatccac
     4921 ccatctccgc catatctctc ttacctggtc atcaccaacc ggcgccccct tttctaaaaa
     4981 ggctgtaggt ttggtagcat ttagccattc agtaatggtc ttcaaggttt ccagagtagc
     5041 aaaccgtcga tcagccatct ctcgagtgta atctttaata tccatggtga ttcctccttc
     5101 acatctttgg ttctcctatt ttactctctc catttctttt tatcaataag cgggtactgc
     5161 gtgcactcga gaatttgaaa ttcgctcttc aggagggatg atagccaacc agcataatta
     5221 ctaaaatggg taaagaaatg tttgaggaca attaagaagt cggcagaagt tttataaacg
     5281 gttatgtgta ggattgtaac accgagatta agatactaaa gagaaatatt ggggtttaag
     5341 gaatgtagag gcttactgga gaaagatact accgcgattc tcaccacaaa gataatgttt
     5401 ccacccaatt tgacagagag ccctcgccct attgacaaac gaggggggaa attatacaat
     5461 ttagctcact ttaatag
//
LOCUS       DAOPSS010000105         5428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12264_length_5428_cov_8.610832, whole genome shotgun sequence.
ACCESSION   DAOPSS010000105 DAOPSS010000000
VERSION     DAOPSS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12264_length_5428_cov_8.610832"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKN31_06500"
     CDS             complement(<1..296)
                     /locus_tag="PKN31_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99609.1"
                     /translation="MFWDIIFLILIFSIYLPALQKKMIEQQRMAAIKSLERKRRSRVI
                     AMIHRQESVSFLGLPIARYINIEDSEKILRAIHLTPEDMPIDLILHTPGGLVLAA"
     gene            complement(317..910)
                     /locus_tag="PKN31_06505"
     CDS             complement(317..910)
                     /locus_tag="PKN31_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOA99610.1"
                     /translation="MVKQMEGKKEVILKTPLSVEEIKFLRAGDKVHLNGVVYVARDLT
                     HQRLIEDLEKGKELPFSFSGSVIYYAGPTPARRGEIIGSIGPTTSSRMDKYLEYFLQK
                     GLRATIGKGERREEVKELLEKYGALYLLACGGAAAYLSTFVVEKEVLDYEDLGAQALL
                     RLKVEQFPLLVAYDAQGGDIFKEGKIKYRNLALYNEY"
     gene            complement(904..1746)
                     /locus_tag="PKN31_06510"
     CDS             complement(904..1746)
                     /locus_tag="PKN31_06510"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOA99611.1"
                     /translation="MKELEFQEIVQQVEKALEELNIISPQLLREKMLEALAAEENDLA
                     REMLSQVMENYVVAEERHLPLCQDTGMVMAKVEKGEEIRIKGGSLRQALDEAIRRAYK
                     NNYFRKSIMSDPFFGKNTGDNTPGVYFFELEEGDELRIDLLVKGGGCDNISSLGILEP
                     GSKPEEVERFVLRELDTKGGQACPPLIVGVGIGGNAPYALYLASRALSRPLGEANPDP
                     RYRELEEKWREDINRLGIGPQGVGGKTTCLEVRIETYPCHIASFPVGLVCSCHVFRQK
                     TLKW"
     gene            complement(2512..3303)
                     /locus_tag="PKN31_06515"
     CDS             complement(2512..3303)
                     /locus_tag="PKN31_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOA99612.1"
                     /translation="MANKYLSREDAPLTPQVWELLDRTMIEVAKAYLSGRRILSWEGP
                     FGLGLKFVSLSNISEGEGVSLSQALSLVEIQRSFRLRKMDLANYERERVTIDLTPLVE
                     ATRECARQEDEIIFQGLTGIPGLLTVTGSQKMKLSSWEETGEAANDIIQAVTLLDEGG
                     FHGPYTLALAPKRYNLLLRIYSQGAKTELEHLKEIVGDGVVKAPFLKKGGVILDSNKN
                     YVSLVVGQDMTVGFIGPEEGDLVFNISESIVPLIREPQAIGILEE"
     gene            complement(3318..3677)
                     /locus_tag="PKN31_06520"
     CDS             complement(3318..3677)
                     /locus_tag="PKN31_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOA99613.1"
                     /translation="MAEFLNPFSGLTPDKKMSLSELIRAIRLSIAAEEEAIHLYTAQA
                     DATDHPLAKKVLLDIADEERVHAGEFLRLLQILTQDEDQFLAEGKQEVEEMREELYQE
                     GKIVEGEPPTVGPLKES"
     assembly_gap    3730..3829
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3830..4089)
                     /locus_tag="PKN31_06525"
     CDS             complement(<3830..4089)
                     /locus_tag="PKN31_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOA99614.1"
                     /translation="MVVVVTPNPCVDKTLFIKENHWGEKIPVQEMQEVAGGKGSNVSR
                     VLKTWGEEAKHLLFLGGYTGNRVKELLEEEKIDTYPVEIESFT"
     gene            complement(4092..5426)
                     /locus_tag="PKN31_06530"
     CDS             complement(4092..5426)
                     /locus_tag="PKN31_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99615.1"
                     /translation="MQYWEEKLDSFDYEERWNAFLKLVEKARKGEIILPPPSPRVNLH
                     FHTFFSFNAYGYSPLHVIWKAKELGLSMAGSVDFDVLDAVPEFQRGGLEVGLPFSSGI
                     ETRVHLPEFPDKEFSSPHEPGVAYYMGNGFTRLPDKGSELEQILWSLKKIAQTRNQKV
                     IEKVNAYLDKVQIDYQEDLLPLTPSGNPTERHIVLAYALKSEALIPDEEERAKFWGEK
                     LAMEKEEVKKLFRDKATFYDFIRSKLIKFGGVGYVKPDAGDFPSRSEVNKMIWGLGAI
                     PSFSWLDGTNSGERDPEMLLEFCLANGVETIFLIPDRAWNIPCEEERKIKLAKLYEIV
                     EETKKRDMPVFVGTELNRYGQKLVDDFDSPYLEPLAPYFLESAWVLWGHVVMEMSSRR
                     GYTSDWAKENFSNRRKRNKFFASLGKNTPAEEKAIKQVGEESTSRLLQQFGE"
BASE COUNT         1512 a         1314 c          893 g         1609 t
ORIGIN      
        1 gcagcaagca ccagccctcc gggagtgtgg agaatcaaat caatgggcat atcctcagga
       61 gtaagatgaa tagctcgcaa tattttttct gaatcctcaa tattaatata gcgtgctata
      121 ggtagaccta aaaagctaac tgattcttgc ctgtggatca tggcaattac ccgcgatctc
      181 ctttttctct ccaaactctt aatcgctgcc attctttgct gctcaatcat cttcttctgc
      241 aaagcaggca ggtaaatact aaaaatgagg ataagaaaga ttatatccca gaacattttt
      301 tacaacctcc cttatatcag tattcattat ataaagcaag attacgatat tttattttcc
      361 cctctttaaa aatatctcct ccttgagcat cataagctac caaaagggga aattgttcca
      421 ctttcaagcg caaaagagct tgagctccca ggtcttcgta atccaacact tctttttcta
      481 ctacaaaagt ggaaagataa gctgctgctc ctccgcaagc cagtaaataa agggctccat
      541 atttctctaa aagctccttc acttcctctc ttctctcccc ctttccaatg gtcgcccgca
      601 aacctttttg cagaaaatat tccagatatt tatccattcg gctactggtg gtgggaccaa
      661 ttgaccctat tatctctcct cggcgagcag gagtgggacc agcataataa ataactgatc
      721 ccgaaaaaga aaagggaagt tctttccctt tttccaggtc ctctatcagg cgttgatgag
      781 ttaaatcacg agccacgtag acaactccat ttaaatgaac tttatcacct gctcgcaaaa
      841 atttaatctc ttccaccgaa agaggagttt ttagaattac ttctttcttc ccttccattt
      901 gcttcaccat ttcaaggttt tctgacggaa aacatgacag ctgcacacta aacccaccgg
      961 gaaactggct atatgacaag gataggtttc tattcggact tctagacagg tagtctttcc
     1021 tcccactcct tgaggaccaa ttcccagtcg gttgatatct tctcgccatt tttcttctaa
     1081 ttcccgatag cgagggtcgg gattggcttc tcctagaggg cgggataaag ctcgagatgc
     1141 gagataaaga gcatagggag cattaccacc tattcctact cctacaataa gaggagggca
     1201 agcttgccct ccctttgtat ccagctccct gagaacaaat ctctctacct cttctggctt
     1261 gctgcccggt tctaaaattc ccagagagct gatattatcg catcctccac ccttaaccag
     1321 aagatctatg cgaagctcat ctccttcttc tagttcaaag aaataaacgc ctggggtatt
     1381 atctccagta ttcttaccaa agaaagggtc agacattatg gactttcgaa aataattatt
     1441 tttataagct ctccttatag cctcatccag tgcctggcgc aagcttcctc ccttaattct
     1501 tatttcttct cctttttcca ctttagccat caccattccc gtatcctgac agagaggaag
     1561 atgtcgctct tccgctacca cgtaattttc cattacctgt gataacattt ctcgagctaa
     1621 atcattctct tcagctgcta aagcttctaa cattttctct ctaagtagtt ggggactgat
     1681 tatattcagc tcttctaatg ccttttctac ctgttgaact atctcttgaa attctaattc
     1741 tttcattttc tacttcctct tcgtagttta attctaaaac cccggagact ccgctttaat
     1801 cctttatttt accaccagta gtggacttct tctttaacat aattattgtg aatctccgct
     1861 attttctccg caacctcctc aaaaacagag aaagttccga tgctcaagag ctttctcgac
     1921 ttgttctggg tctttagctc cgggaataac cgtagcaact tcgggaaaca taagaattca
     1981 tttcccttta actatttcat ccagtatatc aaaagcttca gagttacagt aaagttctgc
     2041 agaaaggaac agagctgcaa aaggcctata gcttctgcac ccaagttaga gaaacttcta
     2101 tccacaagag cattcaagct ttcccgatta taactgaaaa taaaagtgga gaaacagcat
     2161 gtaatgctac tcagatagcc tcttcttcta cctttcccaa tccacttcca atagcccagg
     2221 cgcaaaactg acttctctta ctgttcaacc cgtttgacca aaaaccccgt agttcaagat
     2281 tttatctctc ccattatttt tttataaatt ataacagaaa ataactcaga gcaaagagca
     2341 aagcaaatta acctattaat aaaagaaagg gagataacga aagataccag aagtcttcag
     2401 tcagttgaaa cccgttgaat gcttcgcaaa acaatcttgc tttaggcagg gagacaattg
     2461 taattgtctc cctgcggact tacagggtgt acaattcttg atttttgcca cttattcttc
     2521 cagaatacca atagcttgag gttcccggat aagaggaact atgctctcag agatattgaa
     2581 aactaaatct ccttcctcag gcccgataaa tcctacagtc atatcctgac ccaccactaa
     2641 ggaaacatag tttttgttag aatctaatat tactcctccc tttttgagga aaggagcctt
     2701 tactactcca tctcctacta tttccttcag atgttccagt tctgttttag ctccctgact
     2761 ataaatccgc aaaaggagat tgtagcgctt tggtgctaaa gccagagtat aggggccatg
     2821 aaatcctccc tcatcaagaa gggtaacagc ctgaatgata tcatttgccg cttcacctgt
     2881 ctcttcccag gaagacagtt tcattttttg gcttcctgta acagtcagaa gaccgggaat
     2941 acccgtcagt ccttgaaaaa taatctcgtc ttcctggcga gcgcattccc gagtagcttc
     3001 aacaagaggc gttaagtcta tagtcactct ttctctttca taattggcta aatccatttt
     3061 tctcaaccgg aaagacctct ggatttccac taaagaaaga gcctggctca gtgaaacccc
     3121 ttctccctct gaaatattag acaaagagac gaattttagt cctaaaccaa aaggaccttc
     3181 ccaggaaagg attcgtctcc ccgaaagata agctttagcc acctctatca tggttctatc
     3241 cagcaattcc caaacttgag gggttaaagg agcatcttcc cgacttaagt atttattagc
     3301 catctcttac cctcctttca gctctctttg agaggtccca cagtaggagg ctctccctct
     3361 acaatttttc cttcttgata cagttcttct cgcatttcct ccacctcttg ttttccctca
     3421 gccaggaact ggtcttcatc ctgagtaaga atttgcagca aacgcaaaaa ctccccagca
     3481 tgaactcgtt cctcgtcagc aatatcgagt aatactttct tggccagagg atggtcggta
     3541 gcatcggctt gagcagtata aagatgaatg gcttcctctt ctgctgctat actaagacga
     3601 atagcccgga tgagttctga caaactcatt ttcttatcgg gagttagacc gctgaaagga
     3661 tttaaaaatt cagccatttt cttacctcct taaaaagctt ttaatttatt ctaccacaat
     3721 gaagttatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng taaaggattc
     3841 aatttctacc ggataggtat cgattttttc ttcttctaat aattctttaa ctctattccc
     3901 ggtataacct cctaaaaata aaagatgttt tgcctcctca ccccaggttt taagaactcg
     3961 agatacatta gaaccttttc ctcccgccac ttcttgcatc tcttgaacag ggattttttc
     4021 tccccagtgg ttctctttaa taaacaaagt tttatccacg caagggttag gagtgactac
     4081 cacaaccata actactctcc aaattgctga agcaagcgag aagtagattc ctctcccact
     4141 tgtttaattg ccttttcttc agcaggagtg tttttcccta aagaggcaaa aaatttattt
     4201 ctcttccgcc ggttggagaa attttcttta gcccaatcgc ttgtataacc tctccgagag
     4261 ctcatctcca taactacatg tccccacaga acccaggcac tctctaaaaa gtaaggggca
     4321 agaggttcaa gatagggaga gtcaaagtca tcaactaact tttgtccgta gcggttaagc
     4381 tctgttccca caaaaacagg catatctctt ttttttgttt cttctactat ttcatagagc
     4441 ttagctaatt ttatctttct ttcttcctcg caagggatat tccaggctcg gtcgggaatg
     4501 agaaagatag tctctacgcc attagccaaa caaaattcca agagcatttc cgggtctctt
     4561 tctccactat tagtcccatc taaccaagaa aaagaaggta tagctcctaa cccccaaatc
     4621 attttattaa cttccgatcg cgacggaaaa tccccggcat caggttttac ataacccacc
     4681 cctccaaatt tgatgagctt actgcggata aagtcataaa aagtagcttt atcccggaag
     4741 agtttcttaa cttcttcttt ttccatagcc aatttttctc cccagaactt tgctctttcc
     4801 tcttcatcgg ggatcaaagc ttcacttttt agagcataag caagaactat atgtctctcg
     4861 gtggggttgc cggagggagt aagaggtaac aaatcctctt ggtagtcaat ttgcacttta
     4921 tccaagtaag cattaacttt ttctattact ttctgatttc tggtctgagc aatttttttc
     4981 aaactccaaa gaatttgttc cagctcacta cctttatcag gaaggcgggt aaaaccatta
     5041 cccatataat aagccacccc cggttcatgg ggagagctaa actccttatc gggaaactcc
     5101 ggaagatgaa ctcgagtttc aatacctgag gaaaagggca aacctacttc cagccctcct
     5161 cgctgaaatt caggcactgc atcaagaaca tcaaaatcca cacttcccgc catgctcaaa
     5221 cccagctctt tggctttcca aatgacgtgc aaaggtgaat aaccataagc attaaaagaa
     5281 aagaaagtgt gaaaatgaag attaactcga ggagaaggag gaggaaggat tatctcccct
     5341 tttctagctt tttccactaa tttcaaaaaa gcattccaac gctcttcata atcaaaacta
     5401 tctaattttt cttcccagta ttgaatat
//
LOCUS       DAOPSS010000106         5355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12471_length_5355_cov_8.442264, whole genome shotgun sequence.
ACCESSION   DAOPSS010000106 DAOPSS010000000
VERSION     DAOPSS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12471_length_5355_cov_8.442264"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   5..167
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5..34
                     /rpt_unit_seq="gtttcaatccctcgtaggtaggctacaaac"
     gene            632..1099
                     /locus_tag="PKN31_06535"
     CDS             632..1099
                     /locus_tag="PKN31_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOA99616.1"
                     /translation="MKNKVEKEWEKEFDYFNKRVTPCRRAIMEIFEEEERHLSAEEVY
                     MLLRERGEKVGIATVYRNLDLLSQMGLLHRVNFGDGREHYEMTRVSPPQHHHHLVCTR
                     CGKVIDYSELVREEHEFLDRLERELSSKYDFLIQSHQIYFYGLCESCRSNFES"
     gene            1141..1383
                     /locus_tag="PKN31_06540"
     CDS             1141..1383
                     /locus_tag="PKN31_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOA99617.1"
                     /translation="MEKKQMSLVEMPPGQEGTVVAIDGGQGVVNRLWVMGIFPGRKIT
                     KLASLPAQGPVVIKVGNQELALGRGMAQKVIVEVTR"
     gene            1380..3089
                     /locus_tag="PKN31_06545"
     CDS             1380..3089
                     /locus_tag="PKN31_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOA99618.1"
                     /translation="MKKVVLAGNPNVGKSVIFTQLSGVYAISSNYPGTTVDFSKSTVR
                     KNNTWFELIDAPGTYALSAEAEAEKIAEKLIEEGDIVVNVVDATNLERNLYLTFELLE
                     KKKPMILVLNMWDEAKKRGIEIDREALEEALGVPVIPVVATTGEGIKDLVRALDRAQV
                     PKLIPQDHEKRWEEIGNLLKKVQRFSYREPQWVDFLYRATISPVGGLLIGIGVLIGSF
                     FLIRLIGEGIINYVFDPLFDRFYLPLLEKLDQLLSFSPFLQSLLIGQRIEGVIDFEQS
                     LGVLSTGIYIPIAVVLPYILAFYLILGFLEDIGYLPRLAVLLDGFFHKVGLHGYAIVP
                     TLLGLGCNVPGILGTRVLESEVERFIVATLISVAIPCAAQQALIVGVLGDFGIGPVVL
                     VYSVLFLVWVVTALFLKFVVPGYRPALIIEIPPYRLPYPRAVASKLWMRVREFLKDAL
                     PIVLLGVLGINLLYTTNVFSHLAETFSPFLSRWLGLPGEAIFVLFLGFLRKDLAMGLL
                     IPLGLSPKQLIISSVVLAMFFPCVATFVIILRELGWKRLLQSTVIMVSLSLLVGGLLN
                     VVL"
     gene            3480..4709
                     /locus_tag="PKN31_06550"
     CDS             3480..4709
                     /locus_tag="PKN31_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA99619.1"
                     /translation="MNLKFMSKNLDEEGLKVARDMLSKINPDIRKELVSWSLRHPRYL
                     KSFFHLYQAYNNAQQIRKKELENGLQVPPVLIISITPCCNLRCEGCYASAAGHIKHTD
                     DLKTKAALNVTQWQKIIKEAGELGIFVNIIAGGEPFLFPGLIDLCEEFKEQAFVIFTN
                     GTAITQADYDRLKRSTNLAIVVSVEGSCEYTNKRRGEGVYEKALETIRCLDRIGVPAG
                     ISATITRENFRYWMDADNIDRLIRQNIRILFFLEYIPQSPLPQAGTELADCLAASSLD
                     NTLSESINDHALLLRPEEREEFRSQVLAFRSTKPVFIIHSPGDEEYFGGCVSAGRGFV
                     HVTPAGDLTPCPVSNIATHNLVNSTLREGLASPLFKEICKNEHLLETDGVPCALFAHP
                     EEVSALARLVGAYRTDL"
     gene            4978..>5355
                     /locus_tag="PKN31_06555"
     CDS             4978..>5355
                     /locus_tag="PKN31_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99620.1"
                     /translation="MFVDRRYRNFIIDMDGVIYRGNTPLPHSAEFINFLREIDAKVVF
                     FSNNSTLSRKQYVEKLSNMGIKAREEEIVSSGLITAYCLSKENPGASLYVIGEEGLRE
                     ELGQRGMNLVEVPPYQVDGVVVGM"
BASE COUNT         1460 a          977 c         1285 g         1633 t
ORIGIN      
        1 tgtggtttca atccctcgta ggtaggctac aaacctatat taaactttat tttatggcaa
       61 gctggctatg tttcaatccc tcgtaggtag gctacaaaca aacttcccta tcttgctctc
      121 cgaattgctc cagtatgttt caatccctcg taggtaggct acaaacccgt ttttggggct
      181 taaaataagg atttttaatt ttctatttaa ttttcaaggt gcgcacctga aattattaaa
      241 aactattatt atccttttgt caagtgaaag gagaaaggtt tttttctttc tgtcgatccc
      301 cgggggtttt tacgctaccg gaggtcgaca gaaaattagg tggttatttt gtggttgcct
      361 ttctcttttt ttgccacctg caagcatccg cacttaatga taaaattatt ttatctgaac
      421 tatgtgaggg agctttagct tatttttcaa gcgctggagt ttagcaggaa tctttttagt
      481 tattcaatac agagagattg cccggggcga ctgccagctc ctgccccatt ttttatttcc
      541 cctatcttgc tgatttaccc cggcagtagt ataatactaa aaagaatgat attgataatc
      601 gttatcaaaa tattgtcaag gagataagat tgtgaagaac aaggtagaga aggagtggga
      661 gaaggaattt gattatttta ataaaagagt cactccttgc cggagagcca ttatggaaat
      721 ctttgaagaa gaggaaaggc atctttcggc ggaagaagta tatatgcttt taagggaaag
      781 gggagagaaa gtaggaatag ccacagttta tcgcaatctg gatttgctat ctcagatggg
      841 gcttctgcac cgggtaaact ttggcgatgg cagggaacac tatgagatga ccagggtctc
      901 acctccccag catcatcatc atttagtttg tacccgatgt ggtaaggtga tagattatag
      961 cgagttagta agggaagagc acgagttttt ggatcgcctg gagagagagc tgagtagcaa
     1021 atatgatttt ttaatccagt ctcaccagat atatttttat ggattgtgtg agagttgccg
     1081 atcaaatttt gaatcttaag gtgatggtta gcagggttag cggaaaggcg gagggtaaag
     1141 ttggaaaaga aacagatgtc tctggtagag atgccaccag gacaagaagg aacagtagtg
     1201 gcaattgatg gtggacaagg ggtggtgaat agattatggg tcatggggat ttttccggga
     1261 agaaaaataa caaaattagc ttctctaccc gctcagggac cggtggtcat aaaagtggga
     1321 aatcaagagc tggctctggg aagagggatg gctcagaagg tgatagtgga agtcactcgc
     1381 tgaaaaaagt agttctggct ggtaacccca acgtgggtaa aagtgtcatt tttactcaat
     1441 taagcggggt ttatgctatt tcttccaatt atccgggcac gactgttgat ttttctaaaa
     1501 gtacagttcg gaaaaacaat acatggtttg agttaataga tgctccaggc acttatgctc
     1561 tctctgctga agccgaggca gaaaaaatag ccgagaagtt gattgaagaa ggagatatag
     1621 tggtaaatgt ggtagatgct actaatctgg agcggaatct ttatctcacc tttgaacttc
     1681 tggaaaaaaa gaagcccatg attttggttt tgaatatgtg ggatgaagcc aaaaagcggg
     1741 gaattgaaat agaccgcgag gctttagaag aagctctggg ggtgcccgtt attccagtag
     1801 tagctaccac cggagaggga attaaagatt tggttcgtgc cctggatcgg gctcaagttc
     1861 ccaaattgat tcctcaagac catgaaaaac gctgggaaga aatcgggaat ttactgaaga
     1921 aggtccagcg attttcctat cgagaaccac agtgggtcga ttttctttat cgggctacca
     1981 tatctcctgt aggaggacta ttaattggaa taggggtttt gatagggtct tttttcttga
     2041 ttcgcttaat tggtgagggg ataattaatt acgtttttga ccctcttttt gatcgttttt
     2101 atcttccttt attggaaaaa ttagaccagt tgctttcttt ttctcctttt cttcagagtc
     2161 ttctaattgg tcagagaatc gaaggagtta ttgacttcga acagtctctg ggagtgttat
     2221 ctaccggaat ttatatcccc atagcagtgg ttctgcctta tattctggct ttttatctga
     2281 ttttaggttt tttagaagat ataggatatc ttcctcggct tgcagttcta cttgatggct
     2341 ttttccacaa agttggtttg catgggtatg ctatcgtgcc tactttactt ggcttggggt
     2401 gtaacgtccc ggggatttta ggcacccggg ttttagaaag cgaagtggag cggtttattg
     2461 tagctactct tatatcggta gcgattccct gtgctgctca acaggctctc attgtgggag
     2521 tgctgggtga ctttggaata ggtccggtgg ttttagtata ttccgttctt tttctggtct
     2581 gggtggttac cgctcttttc ctgaagttcg tggttcccgg gtatcgcccg gcgctgatta
     2641 tagaaattcc tccctatcgc ctgccttatc cccgagcggt ggcttctaag ttgtggatga
     2701 gagttcgaga gtttttgaaa gatgctcttc ccattgttct actgggagtt ctggggatta
     2761 atcttctcta tactactaat gttttctctc accttgctga aacttttagc ccttttcttt
     2821 cccgatggtt agggttaccc ggcgaggcaa ttttcgtttt gtttctgggc tttttgcgta
     2881 aagatctggc tatgggttta ctcattcctc tggggctttc accaaagcaa ctgattatat
     2941 ccagtgtggt actggctatg tttttccctt gtgtggctac ttttgtgata atattgcggg
     3001 agttaggctg gaaaaggctt ctccagtcta ctgtaattat ggtttctctg tcgctacttg
     3061 tgggaggact cctgaatgtg gttctataat cttctaataa ggagtgccga atatagcctc
     3121 gtgagtattt agagactatc ctgtttttac ttcataggct ctgtaatgct ttcataagaa
     3181 caattcatct cctgccctgg tgctgttcca gagagcatcg atgtgcactt caatctggtt
     3241 tttagagaaa atacaggttt tgtacctttc ctgtttttga aagactatct tttctttctt
     3301 atccggaaga tgagagatgc tggaatggtt ggaagctaat aagggggaga caaaataccc
     3361 tgcaggttca cactacactg gccagttcga ttgcttgact ctattcagga tatcaaatac
     3421 aatcttccat gtttttattg gagttaaagc agcggggagt tagaaacaag gagaaaacaa
     3481 tgaatttaaa atttatgagt aaaaatcttg acgaagaggg attaaaggta gcaagagata
     3541 tgctcagcaa aataaatccg gatatcagga aagagttagt ttcctggagt ctgcgacacc
     3601 ctcgctatct gaaaagcttt tttcatctgt atcaagccta taacaatgct cagcagatcc
     3661 ggaaaaaaga attagaaaat ggattacaag tcccgcccgt cttaattata agcatcactc
     3721 cctgctgcaa tcttcgttgt gagggatgtt atgcctcggc tgccggccac atcaagcata
     3781 cggacgattt aaaaaccaaa gctgccttga acgttacaca atggcaaaaa ataataaaag
     3841 aggcaggaga attgggcata ttcgttaata taattgccgg tggagagcct tttttgtttc
     3901 cggggttaat agatctttgt gaagagttta aagagcaggc ctttgtaatt tttaccaacg
     3961 gcaccgccat cactcaagcc gactatgaca ggttaaaacg ctccaccaac ctggctatcg
     4021 tagtaagcgt ggagggaagc tgtgaatata ccaacaaaag aagaggagaa ggggtctacg
     4081 agaaagctct ggaaactatc aggtgtcttg accgaatagg tgtgcctgca ggaatatctg
     4141 ccaccatcac ccgagagaat tttagatact ggatggacgc tgataatatc gataggttaa
     4201 ttcgacagaa tatcagaata ttattttttc ttgaatatat tccccaatca cctctgcctc
     4261 aagcagggac ggaattagct gattgtttag cagcatcttc tctggataac actctgtctg
     4321 aatcgataaa cgaccatgct ctcttgcttc ggccggaaga aagagaggaa ttcaggtcac
     4381 aagttctcgc attccgctca acaaaacctg tttttattat tcattctccg ggtgatgaag
     4441 aatatttcgg aggttgcgta tctgcaggaa gaggttttgt ccacgttact ccggcaggag
     4501 accttactcc ctgtccggta tccaatattg ccactcataa ccttgttaac tctaccttaa
     4561 gggaagggct ggccagtcca ttatttaaag aaatatgcaa gaatgaacac ctgctggaaa
     4621 ctgatggcgt accctgtgct ctgtttgccc atcctgagga agtctctgct ctggcaagat
     4681 tagttggtgc ctacaggacg gatttatagg atataaatat ttttgtccgg gctgaagaag
     4741 ctcgaagttt taatgcccct gttcttgata aatctggtta ctaatttctc ttgacctccg
     4801 agagtttaac caaagacctt tcctgaatgg gctgttcata ttatctaata ccgaatgggg
     4861 aatagcggag ggaaaacaaa aagggttgac actgcaccag gcaactagaa gaatagaatt
     4921 tttaaagcga ttctggtaat ataatagatt taataaccag aagaaaggaa aaagagtgtg
     4981 tttgttgata gacgctatag gaattttata atagatatgg atggggtaat ctaccgggga
     5041 aataccccct tacctcacag tgcagaattt ataaatttct tgcgagaaat agatgcaaaa
     5101 gtagttttct tttccaataa ctctaccctt agccggaagc aatatgtgga gaaattgagt
     5161 aatatgggca tcaaagctcg agaagaagag attgtgagct ctggcttaat cactgcttat
     5221 tgtttgagca aagaaaaccc gggagcttcc ctctatgtga ttggggaaga agggttgcgg
     5281 gaagagcttg gtcaacgagg aatgaatctg gtggaagttc ctccttatca ggtagatgga
     5341 gtggtggtgg ggatg
//
LOCUS       DAOPSS010000107         5347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12496_length_5347_cov_8.905140, whole genome shotgun sequence.
ACCESSION   DAOPSS010000107 DAOPSS010000000
VERSION     DAOPSS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12496_length_5347_cov_8.905140"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..915)
                     /locus_tag="PKN31_06560"
     CDS             complement(226..915)
                     /locus_tag="PKN31_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOA99621.1"
                     /translation="MLPLGTLANAGAILIGGMVGMIIRSRLPERISLIVFQGIGLFTL
                     SLGVSLAIKTQNFLLLIFSIVLGSIIGELLDLEKGLNHLGENLKSLIKSKNDRFAEGL
                     VTAFLLFCMGSMTILGAIEEGLGNPPHLLLAKSVLDGFSSIALSASLGAGVIFSIIPL
                     LLYQGGITLFASSLQSFFTDSLILELTATGGLLLIGLGINILEIKKLKVLNMLPSLVV
                     VVILKMLFFHN"
     gene            complement(929..1114)
                     /gene="rpmB"
                     /locus_tag="PKN31_06565"
     CDS             complement(929..1114)
                     /gene="rpmB"
                     /locus_tag="PKN31_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005386334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOA99622.1"
                     /translation="MAKCEICGKTTSFGNKISHSHRVSRKTWRPNIQKVRAVVDGKTR
                     KIKVCTSCLQAGKVKKA"
     gene            1199..3577
                     /gene="recG"
                     /locus_tag="PKN31_06570"
     CDS             1199..3577
                     /gene="recG"
                     /locus_tag="PKN31_06570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOA99623.1"
                     /translation="MIERKIDRELVLKIIEREKQRLDNLSVFGGFSNFLSGWLTERGE
                     GGDFYSLLLNYDNLTLEERSKLLQDMEEKLKSQSDDGDILKVSSPDLPSPEKEKSFSD
                     GEGNLSTPVRYIKGVGPVLEKKLNRLEIKTAEDLIFFFPRYYRDRGEVKPISQLRGEG
                     WETIQGVVTSQSRVATRRGPLLKIAVSDGTGKINLVCFNRNYLAKTLRKGVKVQVSGN
                     FRRSFGNWEVADFDYQLVTKENHRWDRIIPIYSLTEGLSLKKLQSIIYYALDNFLGEV
                     EEILPSSLVARHNFLPKRVAIEELHRPSRSSVAELNSRRSKAHLTIIFEEFLLFALSL
                     KIRRQELADCEVPPCVSDSALVKNFLVQLPFTLTSAQERVVKEIHEDLVSGRLMNRLV
                     QGDVGSGKTLVALLGALQVIDSGRQVAFMVPTEILAEQHYNRFKDLLSSLGVRVGLLK
                     GGTNKEKKKLIEEIRKGEVDLVIGTHALIEENITFPQLGLTIVDEQHRFGVLQRAKLK
                     EKASIPHLLVMTATPIPRTLALTLYGDLDISVVDEKPAGRKPVTTLCFSLKERFKAYN
                     RVRKQIEEGRQAFVVCPAIEESEAEISNVVEVTELLRSSWLSGFRIEMIHGKLPSREK
                     EEIMERFSRGDIDVLVATSVIEVGIDIPNASVMVVENAERFGLAQLHQLRGRIGRGEA
                     AGLCILLAEARTEEARRRMEIMVATDDGFCIAEEDLRLRGPGEFYGVRQHGVPEFHLA
                     DPFEDWSILERANEEAKLILDEDPELKREENRLLKKEINRRFGRYLEFGEIS"
     gene            3578..4141
                     /gene="rsmD"
                     /locus_tag="PKN31_06575"
     CDS             3578..4141
                     /gene="rsmD"
                     /locus_tag="PKN31_06575"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOA99624.1"
                     /translation="MGYLYLVGGKDKGRKIVTPPGKKVRPMPGVMRKAIFEILQEEIP
                     ESVVFDIFAGSGSLGFEALSRGASQVYFLEKDSYLCSLLERNIKTCGFEERARVLRRD
                     FLHSFPFPSRSIKPDIVFVDPPFSMDSKSVLKKLYNFKVILEDALIVFRYPEEQDPFR
                     DCDFFKIEDRRKYGRSILIFGSFKANS"
     gene            4141..4653
                     /gene="coaD"
                     /locus_tag="PKN31_06580"
     CDS             4141..4653
                     /gene="coaD"
                     /locus_tag="PKN31_06580"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOA99625.1"
                     /translation="MKERLTLIAIYPGSFDPLTNGHLDVIERGRKIFDRLVVGILKNP
                     HKQPLFTSEERKTMIEEAVKPFSNVEVEIFDGLLVNFARERGCQVILRGLRAISDYEY
                     ETQIALINRKMAPEIETLFLPTSAEFSYLNSTIVKEIASFGGCIRGLVPPAVEKKLRD
                     KFDRGNLSNF"
     gene            4725..>5347
                     /locus_tag="PKN31_06585"
     CDS             4725..>5347
                     /locus_tag="PKN31_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOA99626.1"
                     /translation="MDILSEIRKRAKALQKRIALPESQDERVLKAAEIAQKEGIASVV
                     LLGEEGTIAEKAQELNLDLSGVEIVNHLRDEKREFYIDDLYQLRKNKGLSREGAEDLL
                     KSPMYYACMMLRENRVDGVVGGAILSSSDVIRPALQIIKTAPGIKLASSCFLMVVPDC
                     PYGENGIFLYADPALNPNPDAEELAHIAITTARTAVFLLDAEPRVAML"
BASE COUNT         1586 a          917 c         1310 g         1534 t
ORIGIN      
        1 ggaacctaat gtttgggtat tacatgaggt aaatgcgtca taggccgggc tactgggtat
       61 aatagaaagg tttgtgcttc caatgcgctt cacgcaggta aaatgactca atatcaagat
      121 aaagagagat ttctctgtat ttagagattg attgcaaata taatctaaag ctacctcttt
      181 tttctattat atccttcagg gatgcaatta cccgggtttt gtggtttaat tatgaaagaa
      241 tagcattttt agaattacca ccactacaag gctgggtagc atgttgagca cttttaactt
      301 ttttatttct aagatgttaa ttccgagacc tataaggagc agtccccctg ttgcggtaag
      361 ctccagtatc agggaatcgg taaaaaaact ttgaagagaa ctggcaaata aagttattcc
      421 accctggtag agaagaagag gaatgatgga gaaaatcacc cccgctccta aagaggcgga
      481 tagagcaatg gatgagaaac catccagcac cgatttagct aaaagaagat ggggagggtt
      541 acccaggcct tcctctatgg ctcccaagat agtcatagag cccatgcaaa agagcaaaaa
      601 agcagttacc aaaccctcag cgaaacgatc attttttgat ttgataaggg attttagatt
      661 ttctcccagg tggttgagcc ctttttctaa atcgagcaat tctcctatta tggagcccag
      721 gacgatactg aatattaaaa gcaaaaagtt ctgggttttt atggctaagg atactcccag
      781 agaaagagta aatagaccaa tgccctggaa gacgataagc gatatccgct ccggtaaccg
      841 ggagcggata atcattccta ccatgccgcc tataagaatt gctccagcgt tagccagggt
      901 tcctaagggt aacataaaaa acggtaattt acgctttttt aacttttcct gcttgcaagc
      961 aagaagtaca aacttttatt tttctggttt tgccgtctac aactgctctc actttctgaa
     1021 tgttgggacg ccaagtcttt ctgcttaccc gatgagagtg gcttatttta ttaccgaagg
     1081 aagtggtttt accacagatt tcacattttg ccattgttat tactcctttc ctttctcaga
     1141 gtcgggtaaa aatatagcag ttttgaagat gtaatgcaag atgggttaaa atagaaaagt
     1201 gattgagaga aaaatagata gagaattagt actgaaaata atagaaagag aaaaacagag
     1261 actggataac ctctccgttt ttgggggatt cagtaacttt ttgagtggct ggcttacaga
     1321 acggggagag gggggagatt tttattctct tcttctgaac tacgataatt taactctgga
     1381 agagagaagc aaactactgc aggatatgga agaaaagctg aaatctcaaa gtgacgatgg
     1441 tgatattctt aaggtctctt ctccagattt accttcacca gagaaagaaa aaagtttttc
     1501 tgatggggaa gggaatctct ctactccggt gagatacatt aaaggagtag gtccggttct
     1561 ggagaaaaaa ttaaaccgtc tggaaatcaa aacagcagaa gaccttatct ttttctttcc
     1621 tcgttattat cgggaccgag gagaggtaaa acccatttct caactccggg gagaaggatg
     1681 ggaaactatc cagggagtgg ttacttctca aagtcgggtg gctactcgac gggggccatt
     1741 acttaaaatc gccgtgagcg atggaacggg taaaataaac ctggtgtgtt ttaatagaaa
     1801 ttatctggcc aaaactttgc gaaaaggcgt gaaagttcaa gtttcgggaa attttcgtcg
     1861 gtcttttggt aactgggaag tagctgattt tgactaccag ctggtgacga aagaaaatca
     1921 tagatgggat agaataattc ccatctatag ccttactgaa gggttaagtt taaaaaagct
     1981 gcaatccatt atttattatg ccctggataa ttttttaggg gaggtagaag aaattcttcc
     2041 ctcttcttta gtggcaaggc acaatttttt acccaaaagg gtagctatag aggaactgca
     2101 ccgtccttcc cgctcttcag tagcggaatt aaactcccga cgcagtaaag ctcacttaac
     2161 tatcattttt gaggaatttt tgctttttgc tttaagtttg aaaatccgtc ggcaggaatt
     2221 agcagattgt gaggtccctc cctgtgtttc tgattcagcg ctggtaaaaa attttttagt
     2281 tcaacttccc tttaccctga cctctgctca agagagggta gtaaaagaga ttcatgagga
     2341 tttagttagt ggacggctaa tgaatcgttt agtgcaggga gatgtgggtt caggaaaaac
     2401 actggttgct ttacttgggg ctctccaggt tattgattcg ggaagacagg tagcttttat
     2461 ggttcctact gaaattttag ctgaacagca ttacaaccgg tttaaagatt tattgtcttc
     2521 tctgggggtg agagtggggc ttctcaaggg gggaaccaac aaagaaaaga aaaaattgat
     2581 agaggaaatc agaaaagggg aagttgatct ggtaattggt actcacgccc ttatcgagga
     2641 aaacattact ttcccccagc tgggccttac tattgtggat gaacaacatc gctttggggt
     2701 gttacagagg gcaaaattaa aagagaaagc ctctattccg caccttttgg tgatgactgc
     2761 cactcctatt cctcggacgt tagcattaac tctttatggg gatttagata tttctgtggt
     2821 agatgaaaaa ccagcgggca gaaaaccggt gactactctc tgtttttctc ttaaagagag
     2881 gtttaaggct tataatcgag tgcgaaagca gatagaggaa ggaagacagg cttttgtggt
     2941 atgtccggcc atagaagaaa gtgaggcaga aatcagtaat gtagtagagg taacagagct
     3001 tttacgctct tcctggcttt cgggctttcg aatagagatg attcatggta aactcccttc
     3061 tcgggagaag gaagaaatca tggaacgttt ttctcgaggg gatatagatg ttttagtggc
     3121 tacttcagtt attgaggtgg gaattgacat tcccaacgcc agcgtgatgg tagtagaaaa
     3181 tgccgagagg tttggcctgg cacaactgca ccagttgaga ggtagaatcg ggagaggaga
     3241 ggcagcaggc ctctgtatac ttttagctga agcccgtact gaggaagctc gccggcggat
     3301 ggaaataatg gtggctaccg acgatggttt ttgcattgcc gaagaggacc ttcgcttgcg
     3361 aggaccgggt gaattttatg gagttagaca gcacggggtg cctgaatttc acctggctga
     3421 cccttttgaa gactggtcta ttctggagag ggctaacgaa gaagcaaagt taatccttga
     3481 tgaagaccca gaattaaaaa gagaagaaaa tcgtttatta aaaaaggaaa ttaacaggag
     3541 atttggccgt tatctggagt ttggagagat aagttagatg ggttatttgt atctggtggg
     3601 aggaaaagat aaagggagaa agatagtcac ccccccgggt aagaaggttc gtcctatgcc
     3661 cggggttatg cgtaaggcaa tatttgaaat tttgcaagaa gaaataccag aaagtgtggt
     3721 ttttgatatt ttcgctggaa gcggctctct gggttttgag gctctaagtc gaggagcaag
     3781 ccaggtttat tttttagaaa aagactccta tctttgttcg cttctggaga gaaatataaa
     3841 gacctgcggt tttgaagaaa gagcccgggt tttgagaagg gattttttgc attcttttcc
     3901 ttttccttct cgaagcatta aaccggacat cgtttttgtg gatcctcctt tttctatgga
     3961 tagcaaaagc gttttgaaaa agttgtataa ttttaaagtg attctggagg atgctctgat
     4021 agtatttcgt tatcctgaag aacaggaccc tttccgagac tgtgattttt ttaaaataga
     4081 ggatcgcaga aagtatggaa ggagcatttt aatattcggc tcttttaagg ccaattcttg
     4141 atgaaggaga gattgacttt gatagcgata tatccgggga gttttgaccc tctgactaat
     4201 gggcatctgg atgtcattga acgaggaaga aagatttttg atcgtttggt agtggggata
     4261 ttaaaaaatc cccataagca gcctcttttt acctcagaag aaagaaaaac catgattgaa
     4321 gaagcagtaa aacctttttc taacgtggaa gtagaaattt tcgatggttt attggtcaac
     4381 tttgctcgtg agagaggatg tcaggttatt cttcgaggtt tgagagctat ttctgattat
     4441 gagtatgaaa ctcagattgc tctcattaat cggaagatgg ctcccgagat agaaactttg
     4501 tttttaccca ccagtgccga attttcttat cttaactcaa ccatagtaaa agagattgct
     4561 tctttcggtg gttgtatcag ggggttagtg ccgccagcag tagaaaagaa attgagagat
     4621 aagtttgata ggggaaatct ttctaatttt taactttaat agaggagata gcttccttta
     4681 aatggaaaag ttatacatac tacttataaa agggagtaga taaaatggat atattgagcg
     4741 agatcagaaa aagagctaaa gcactacaaa aaagaattgc tttgcctgaa agccaggatg
     4801 agcgagttct gaaagcggca gagatagctc aaaaagaggg catagcttct gtagttcttc
     4861 tgggagaaga gggaacgatt gccgaaaaag cgcaagagtt gaatcttgac ctctcggggg
     4921 tggagatagt taatcactta agagatgaga aaagagaatt ttatattgac gacctttacc
     4981 agttgaggaa gaataagggt ctttcccggg agggagctga agacttatta aaaagcccca
     5041 tgtattatgc ctgtatgatg ttgcgggaaa atagagttga tggggtagta ggaggagcga
     5101 ttctatccag ttctgatgtg ataagaccag ctctacagat tattaagaca gcgccgggaa
     5161 ttaaattagc ctctagttgt ttcttaatgg tggttcctga ttgtccttac ggagaaaatg
     5221 gcatttttct gtatgccgac ccggcattga atcccaatcc cgatgctgag gagttagctc
     5281 atattgctat tactaccgct cgcactgccg tttttcttct ggatgctgaa ccccgggtgg
     5341 cgatgct
//
LOCUS       DAOPSS010000108         5270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12717_length_5270_cov_6.994823, whole genome shotgun sequence.
ACCESSION   DAOPSS010000108 DAOPSS010000000
VERSION     DAOPSS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12717_length_5270_cov_6.994823"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..429)
                     /locus_tag="PKN31_06590"
     tRNA            complement(353..429)
                     /locus_tag="PKN31_06590"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(393..395),aa:Arg,seq:acg)
     gene            complement(483..1811)
                     /locus_tag="PKN31_06595"
     CDS             complement(483..1811)
                     /locus_tag="PKN31_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99627.1"
                     /translation="MNKKIAFFVLAGILVLGGGISWAQAPLFREQLVYSLTSFDGSGF
                     SKSFCPQSEETLYFIANFNSVVNPRKTIVYYWPITRRYMAGFSTLNEEVPGKLEIWQG
                     GKLINTLEKQKYVLYYPEGYWSEKATIYLGEDAQEYYDEYKAAVDEFNKKLQEYYQAM
                     NYYQQQLNNFFEEVRRRREAGETGPLDISIPQQPEPPEFVKFYVTEPAEGFILNLPVG
                     NYEIRVRMEDGTIFEGSEKNLVVFTARRREGVGYEIIPGNRWTKRENCDDPSWIIHAV
                     GKNELYFNPFYQEEYNELYYNKLKDPQNIGRLEKWKWVHVDPVDDAQVLLGRGEEILQ
                     TVDKAPYFVKQVPGPELGYEIIPYDEELKMSGYQPTFESYKVTLGEDLVGADYQISTI
                     SRETGEHIPKSERTIRFLKKENGAYLYAVAFLPLVAGAIIIISRSYRVEK"
     gene            complement(1811..2629)
                     /locus_tag="PKN31_06600"
     CDS             complement(1811..2629)
                     /locus_tag="PKN31_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOA99628.1"
                     /translation="MAKKVTRTLFKRDFLYTLYSWGYYAAIFASFLVSSFILKNFIDG
                     IREEDILVSAYPLHQPLYITLIIISIYLVIVSAISISREREQGTLEVLFYGPVTAGNF
                     LWSKYFKDLLLGIIALGFTAVYFYLVSCLTNLGFTTGLIKALFMGVFIISCVVSFGLF
                     ISSLTGRIRSSVVTLLAILGAFLAVQFAYGALLGLGEEATISLPLLYLRQTLSYIFQG
                     INWISPFAFLSRGLDAVVLESWSLYLANIVYCLIYSLIFMALSIFTMNRRGVKA"
     gene            complement(2632..3354)
                     /locus_tag="PKN31_06605"
     CDS             complement(2632..3354)
                     /locus_tag="PKN31_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99629.1"
                     /translation="MKKKVGLSIFLAIVGVVVVLFVVSYFTIRLNYGVLQGKVTDHYS
                     HDVVRRLHLTIDGRSDILFQSKDYQFTHLPPGKHTLRAEAPYYYPFSEELEIKRGVNV
                     FDFTMKGKEIPDLAGIICFADPVDRGIQIEIRFKDSKGVGISDFPALPLQLEGKLYVR
                     EGEDEDNYSRGRLIFEGPIELFWDPNAYLSRNKGLITWDKIQIDPEEEKYGIMELVLR
                     TPQGDFEDVIEDVEFQSPKKEE"
     gene            complement(3351..4298)
                     /locus_tag="PKN31_06610"
     CDS             complement(3351..4298)
                     /locus_tag="PKN31_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99630.1"
                     /translation="MIQTVNLTKEFDGFKAVDNLNLQVDKGDICGFLGPNGAGKTTTI
                     NMILGLMPPTSGKIFLLGEEFNPGKVEVKERIGVVSESLYFYKEMTAREYLLFFGELY
                     QVPKASRRIEELLGALGLINDQNKKVGAFSKGMQQKLAFARALLHDPELLILDEPVAN
                     LDPSSIKQIRDLIEEENHRGKTVFISSHLLSEVERLCKKVAIINKGKLLAEDTMDNIR
                     RRLTDSVEMEIELDKEAESIASSLEKLDFLKGFSVEGHKLEVSLKTDRDYRPELSQFL
                     YQNGYLPLSMKAKEMSLEEAFITITQQNISLLARGEEAA"
BASE COUNT         1523 a         1235 c          992 g         1520 t
ORIGIN      
        1 ttttctcgtt tatttgcttt taaagctcac actgtaggcg tctcgctcgc aaagataccc
       61 cgaaaaaatt tccacagaat tataaatgag gggaatattc tccctacaac ccctcctttt
      121 tttgtctttg cgaagaggaa attatgaggc aacctcgctt ttttctaatt aaaaaacctt
      181 aaaagagcaa gaaccactgg gggaaaagca ttcctcttga tgctagaatg cccggagaaa
      241 ttgcacaata cggcagaaaa gagaaacaga gcgtgaagta aggaaacacc gccatgaatt
      301 ttgcagttgg ctacaaagtg agctgaagat gaaatgattc acaaaaaaac actggcgcgc
      361 ctggagggat tcgaaccccc aacctacaga tccgtagtct gttgctctat ccagttgagc
      421 cacaggcgca ccttacctca ccataattat atttctattc agctataaaa tcaactgtct
      481 ctctacttct ccacccggta agaacgactg attattataa tcgctcctgc tactaaaggc
      541 aagaaagcca cagcgtaaag gtaagctccg ttttcctttt tcaagaaacg aatagtccgc
      601 tcgcttttag ggatatgctc tccagtttct ctggagatgg tagatatctg ataatcagcc
      661 ccaacaaggt cttctcccag agttacttta tagctttcaa aagtgggttg ataaccactc
      721 attttcagtt cttcatcata ggggattatt tcatacccca gctctggtcc gggaacctgc
      781 ttcacaaaat aaggagcttt atctactgtt tgcaagatct cttctcctct tcccagaaga
      841 acttgagcat cgtctaccgg gtcaacatgc acccatttcc acttttccag ccgaccaata
      901 ttttgagggt ctttaagctt attataatac agctcattgt attcttcctg ataaaaggga
      961 ttgaagtaga gttcgttttt acctacagca tggataatcc aggaagggtc gtcacagttt
     1021 tctctcttgg tccagcggtt tcccgggatt atttcatatc ccactccctc tcgccggcga
     1081 gcagtaaaga ctactaaatt cttctcactt ccttcaaaaa tagttccatc ttccatcctt
     1141 accctgattt catagttacc cacaggaagg ttaaggataa aaccctctgc tggctcagta
     1201 acatagaatt ttacgaactc aggaggctct ggttgctggg gaatagaaat atcaagaggt
     1261 ccggtctcac cagcttcccg tcggcggcgc acttcttcaa agaaattatt cagctgttgc
     1321 tgataataat tcatagcctg ataatactct tgaagttttt tgttgaattc atctactgcc
     1381 gccttatatt cgtcgtaata ctcttgagca tcttcgccca gataaatcgt ggctttttct
     1441 gaccaatacc cttcggggta atagagaaca tacttctgct tctccagagt gtttatcaat
     1501 tttccaccct gccagatttc caatttcccc gggacttctt catttaaggt gctgaacccg
     1561 gccatatacc ggcgagttat aggccagtag taaacaatgg tttttcgggg gttcaccaca
     1621 ctattaaaat tggcaataaa gtacaaagtt tcctcgctct gaggacagaa gcttttactg
     1681 aagccagagc catcaaaaga agtcaaagaa taaaccagtt gttcccgaaa aaggggcgcc
     1741 tgagcccagg aaatgcctcc tcccagaact aaaatacccg ctaaaacaaa gaaagctatc
     1801 tttttattca tcaggcttta accccccttc gattcatagt aaatatagaa agagccataa
     1861 aaatgaggga ataaatcaga caatagacta tgttagccaa atacaatgac cagctctcta
     1921 aaaccacggc atccagtccc cgacttaaga aagcaaaagg tgatatccag tttatgccct
     1981 gaaaaatata agagagagtc tggcggagat agagaagggg gagagatatg gttgcctctt
     2041 ctcccaggcc taaaagagcg ccataggcaa attgaacagc caaaaaagct cccagaatag
     2101 ctaaaagagt gaccactgag ctgcgaatcc gaccggttaa agaagagatg aaaagcccga
     2161 agctcaccac acaagatatg ataaaaaccc ccataaacag agctttaatc aaacctgtgg
     2221 tgaaccccaa attggtaaga cagctcacta aataaaagta aacagcagtg aagcctaaag
     2281 ctatgattcc taaaagtaaa tccttgaaat atttactcca gagaaagttt cccgctgtca
     2341 ccggtccata gaaaaggact tctaaagtcc cttgctccct ttctcgagag atggaaatag
     2401 ccgatactat caccaaatag atggagataa taattaaggt gatataaaga ggctgatgca
     2461 ggggataagc cgaaactaaa atatcctctt cccggatacc atctataaag ttttttaaaa
     2521 taaaagaaga aaccaggaaa gaggcaaaaa tggctgcata atatccccag ctatacaagg
     2581 tatagagaaa atctctttta aaaagagttc tggttacttt ttttgccatt ctcattcctc
     2641 ctttttgggt gactgaaact ctacgtcttc aataacatct tcaaaatctc cttgaggggt
     2701 gcggagcacc agttccatga tgccgtattt ttcttcttca ggatcaatct gaattttgtc
     2761 ccaggtaata agtcctttat tgcgagaaag ataagcattg ggatcccaga aaagttcaat
     2821 tggtccttca aaaatcaacc ttccccggct gtagttatcc tcatcttctc cctctctaac
     2881 gtagagtttg ccctccagct gaagtggcag agcaggaaag tctgatattc ccaccccttt
     2941 agaatcttta aagcggattt caatttgaat gcctctgtcc acggggtcgg caaaacatat
     3001 gatgccagct aaatcaggga tttcttttcc cttcatggtg aagtcaaaaa cattaactcc
     3061 tctttttatc tctaactctt cagaaaaagg ataatagtag ggagcttcag cgcggagagt
     3121 atgctttcca gggggaagat gggtaaactg ataatctttg ctctgaaata aaatatcaga
     3181 acgcccatca atggtgagat gtagtcttct aaccacgtca tgcgaatagt ggtcagttac
     3241 tttgccttgc agaaccccat aattcagccg gatggtaaaa taggaaacca caaataaaac
     3301 cacaacaact cctacaatag ccagaaaaat agagagacca acttttttct tcatgccgct
     3361 tcctcccctc gggccagtag agaaatattt tgctgggtaa tggtgataaa ggcttcttcc
     3421 agactcattt ctttagcttt catagaaaga ggaagatatc cattttggta aagaaattga
     3481 gacaattccg gacgatagtc ccggtcagtt tttaaactca cttccaattt gtggccttct
     3541 acgctaaatc ctttcaaaaa gtccagcttt tctaaagagg aagcaatgct ttccgcctct
     3601 ttatctaact ctatctccat ctctacactg tcggtcaatc ttcggcgaat attatccatg
     3661 gtatcctcag ccaaaagttt tcctttattg ataatagcta cttttttgca taacctttct
     3721 acttcagaaa gaaggtgaga agagataaaa accgttttcc cccggtggtt ttcctcttca
     3781 attaagtctc gaatctgctt gatgctgctg gggtctaagt tagctactgg ctcatccaga
     3841 atgaggagtt ctgggtcatg caaaagagca cgggcaaagg ctaacttttg ttgcatccct
     3901 ttggaaaaag ctcctacctt cttgttctga tcgttaatca atcctaaggc tcccaacagc
     3961 tcttcaattc ttctggaagc cttgggcacc tggtagagtt ctccaaaaaa gagaagatat
     4021 tctcgagcag tcatttcttt ataaaaatac aaactctcag acaccactcc aattctttct
     4081 ttcacctcca ctttaccagg attgaattct tctcctaaga ggaaaatttt accgctggta
     4141 ggaggcatca accctaaaat catattgata gtggtggtct tccctgctcc gttggggcct
     4201 aaaaaaccac atatatctcc tttatctacc tggagattta agttatcgac agccttaaag
     4261 ccatcaaatt ccttggttaa gtttacagtt tggatcatga aatttccctg cctcctcttt
     4321 tttttaagct actttatttt aacagaaaga aaaagcaaaa ccactttttt ttagcttctc
     4381 attctataag cgtatctcaa gagaagagcg atagttcctt aaaaatttct ctttaataca
     4441 tggcaatatc tttttttcgc gctccactca tcttccctac cggggaatta ttgggggcaa
     4501 tttcacccgg catgtctttg ccagagtcac tttctcgcca ccccatgaga aatcattaat
     4561 aatagatttc tgataggcct gttcaggagt gacgagcaag gtgctttccc tccgggttat
     4621 tttttatctt tttcttttgt cttttcgtgt tttccggggt ttataagggg gcggcgcgat
     4681 tcaccaccct caccctctcc ccgcaagctc tccccggaag ctctctaaac cgtccctctc
     4741 actccgaggg cttctctttt cgtcatacct gaaatcttta atcgggaatc taaatgcttt
     4801 taaaactgtg gacccctgat agaaccattc aggggtgacg acctccctct ttcccgaaat
     4861 ttaggcggga atccacattt ttgcttcttc tttgattttt taaagccgtg gacccctgat
     4921 agaagcattc aggggcaggc taaaaacggc ttgaaaattg ccggggtgac aaacaagggg
     4981 tgatagaagg attcaggagt gacgacctcc cttctcgtca tgcctgaaat ccttaatcag
     5041 gcatccatgt ttttgctttt tctttttgtc cttctattgt gaaccatagg gatgtctcct
     5101 tccattgttt agattcctcg tagaaacact caggaatgac ctcccccgcc gtcattcccg
     5161 agatcttcaa tcgggaatcc aagtttttgc tttttccttt ggttttcaaa taccatggac
     5221 ccctgataga accattcagg ggcaggctaa aaacggctta aaaattgccg
//
LOCUS       DAOPSS010000109         5254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12767_length_5254_cov_7.797076, whole genome shotgun sequence.
ACCESSION   DAOPSS010000109 DAOPSS010000000
VERSION     DAOPSS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12767_length_5254_cov_7.797076"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            714..1115
                     /locus_tag="PKN31_06615"
     CDS             714..1115
                     /locus_tag="PKN31_06615"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOA99631.1"
                     /translation="MAKLTKSMKEAIDQLAQQIISSAAYQNFQEKRALLANDPNFLSL
                     YQQLEEKQDMLDKLADSEEDIPDEYLEELEEISKQLLSEPLFVDYLKAQDQLISLLEL
                     INDELSSLLGFDFADSINFLKEEEEGEEFWE"
     gene            complement(1873..2769)
                     /locus_tag="PKN31_06620"
     CDS             complement(1873..2769)
                     /locus_tag="PKN31_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOA99632.1"
                     /translation="MFPSHPIAIERWAKSLVESYRKTEKITNIENKHFPSRDKVIEIS
                     EELRELLFPGYLGRTNLSWLNAEYFVGGKLDYIFGELSREIAFSLGDKSPEGEERAYR
                     ETEVFFGKLPAVREMLEDDVQAAFDGDPAAKSLDEVIFSYPCLVAISIYRLAHELFIQ
                     GIPLIPRMMTEYAHSATGIDIHPGAKIGRSFFIDHGTGVVIGETCEIGNNVKIYQGVT
                     LGALSFPKDERGKIIRGIKRHPTIEDDVTIYAGATILGGQTIIGKGSVVGGNVWLTHS
                     IPPYTKVAIEEPRLKISENQKL"
     gene            complement(2784..3011)
                     /locus_tag="PKN31_06625"
     CDS             complement(2784..3011)
                     /locus_tag="PKN31_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOA99633.1"
                     /translation="MDCKEVLGKIYLYLDNEILTESERRQIEEHLKWCFKCCQMYELE
                     RNLENLIRINGRNDEVPASLFFRIENVIAQF"
     gene            complement(2990..3586)
                     /locus_tag="PKN31_06630"
     CDS             complement(2990..3586)
                     /locus_tag="PKN31_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA99634.1"
                     /translation="MVSTKEEEKKYFQELLNPELGALFRTALRMTRNREDAEDLVQET
                     VTKAFAAFDRFEKGTNFRAWIFRILTNTFINNYYRVRDRQKLPSLDEMEEESFFQPIA
                     EGITPEEALLNTLTKEDILKAIEDLPVEFRAVVVLVLVEGFSYKEAAEILDIPIGTVM
                     SRLWRGRRLLQKSLWEYSSTRRNDSERKNQEGWTAKKS"
     gene            complement(3602..3907)
                     /locus_tag="PKN31_06635"
     CDS             complement(3602..3907)
                     /locus_tag="PKN31_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99635.1"
                     /translation="MQELPLKCKNCKALITLEDVTEIGIYKIPAYRGSGYIRYICPNC
                     KDFCEVNFNLEKLEDVARITTKKKKKAKPKGQITIDEIIQFHKELENLKNADFLKDS"
     gene            4260..5027
                     /gene="cas6"
                     /locus_tag="PKN31_06640"
     CDS             4260..5027
                     /gene="cas6"
                     /locus_tag="PKN31_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOA99636.1"
                     /translation="MRIKVTFQSDRKICLPTHHNHIIQGFIYSNLDRALSNMLHSEGY
                     KLGSRTFKLFTFSRLLGKMNYNPQEGFTFYPPVSLLIASPKVDILESLSHFLVKRREL
                     SLGNNKVWTESVEVMMEKKFTKRVQVEMLSPVVTYSTLYTQEGKKKTYYYNPQEKEFG
                     EKIRDNLIKKYLLVNDSPLDDNLQFDIVPLRVNKNHEKIIVYKQTVIHGWMGQYEITG
                     NPELIKVSYDAGLGSKNSSGFGMWEVWREDFPEQRIP"
BASE COUNT         1568 a         1108 c         1061 g         1517 t
ORIGIN      
        1 cctttcattg tgaaccatag gcgtgtctcg caccattatt cccgaaattt agtcgggaat
       61 ctaaatactt ttttaaacca tggacccctg atagagacat tcaggggtga cgaatgaggg
      121 tgatagaacc attcaggaat gacgaacaac gggatgatag aagcattcag gaataacgaa
      181 caaaagcgac aaaaccattc aggggtgacg acacggaggc ttttccctcc gtgttatttt
      241 ttatcttttt cctttgtctt tttgtgtttt cgtcttttcc gggggtttat aagggggcgt
      301 tgccccctca tagtactttt ctgtcattcc cgaaattttt tatcgggaat cctcccccca
      361 ccgtcattcc cgaaatcttt aatcgggaat ccaagttttt actttttctt tggtttttaa
      421 aaaccatgga ccccggctta aagattgccg gggtgacgag gaggtgggaa tccactggtg
      481 aatgcttttt ttagttttta aatactgtgg acccctcgta gaggcattca ggggcagcgg
      541 cctcttatag ttctaatcaa aaggggaaaa gagggggttt gtccctcgaa agaaagagga
      601 cacgaaaggg ataatttgaa tttttaaaaa gaaagtttag aaaaattcta tatggtataa
      661 ccaagtttaa tcttttctgt tacaatagat aaaattatga aggaggtgca cccgtggcaa
      721 aactgaccaa aagcatgaag gaggctattg accaattagc tcaacaaatt atatcttctg
      781 ccgcttacca gaatttccag gaaaaaagag ctcttttagc taacgaccct aactttcttt
      841 ctctttatca acagttagag gaaaaacaag acatgctgga caaattggcc gatagtgaag
      901 aagatatacc cgatgaatat ttagaagaat tagaagaaat tagcaaacaa ttgctttctg
      961 aacctctgtt tgtggattat cttaaagccc aggatcaatt gatatctcta ttggagttga
     1021 ttaatgacga acttagttca ttattgggtt ttgactttgc tgactcgatt aacttcttaa
     1081 aagaggagga agaaggagaa gaattctggg aataagtttc ctccagcttg aggagtaaat
     1141 accttaccct tcccatatcg ggcaaatggt cgagggaagt cttgtgctca tcaaactgaa
     1201 actgacggca gctatcagga ggagtaaaag aatcagggat tttaacacca gcatataaag
     1261 tgttccatcc catttctcgc aagcgtttag cgacagaagg ctcgcatatc tcttctatgg
     1321 gttggttttt ctttattact gtgcacttcc cggtgcttat atccactccc ccaccaagaa
     1381 acgagctggc ggcaggttca aaaaagatac aatatcgaca gacatcaaga cgagtgtggc
     1441 acttagggca gtaaagagga cgactcttta ctgctatccc ctcgttgggg atagttttca
     1501 aagtttcagg gatttctatc cgacaaaaag gacataaaaa agaagaaggc caggtaaccc
     1561 tggtaatttt tggtgattct ttaagaagtt ggattttttc ctcatcggta agaggtagct
     1621 gtggttttct cacccactgg aatttaggat agtggaagaa ggcttctatt ccgtacaata
     1681 aagaacggaa gaaagaaatt cctttctggc gagaaaaaga aaaggcagaa acagcgtaaa
     1741 aaaaggaaag ggaaaaccag aaaataggcc agatatacag aaccactatt tttaaaatag
     1801 gaaagggaaa gaaaagatat aatataaccc cacccagcca gatgagaaga aaaataaggt
     1861 ggcgacgagc actcatagtt tttggttttc tgatattttg agacgaggtt cctcaatggc
     1921 taccttggta taaggaggaa tagagtgagt aagccagaca ttacccccta ctacactgcc
     1981 cttcccgatt atagtttggc ctcccaggat ggtagctcca gcatagatag ttacgtcatc
     2041 ttcaatggta ggatgacgct taatccctcg aataatttta cctcgttcat ctttggggaa
     2101 agatagagcc cccagtgtca ccccctggta aattttaaca ttattgccaa tttcacaggt
     2161 ttctccaatc accactcctg ttccgtggtc aatgaaaaag cttcgaccga ttttggcccc
     2221 ggggtgaata tctataccag tcgctgagtg agcatattcg gtcatcattc gaggtataag
     2281 aggaattccc tgaataaaaa gctcgtgagc caggcgatag atcgagatag ccacaagaca
     2341 ggggtaactg aatattacct catccagact tttggccgcc gggtcaccgt caaaggcagc
     2401 ttgaacgtcg tcttctaaca tctcgcgcac cgcaggtagc ttaccgaaaa atacttccgt
     2461 ttcacgatag gctctttctt ccccctccgg tgatttatcg cccagagaaa aagcgatttc
     2521 tcggctcaac tcgccaaaga tatagtctaa tttgcccccc acaaaatatt cagcgttgag
     2581 ccaggataga ttggttcttc ctaaataacc gggaaagagc aattccctca attcctcaga
     2641 aatctctatc actttatctc gagagggaaa atgcttgttc tctatattag ttattttttc
     2701 tgtcttccgg tagctttcca ccagagactt ggcccatctt tcgatggcta tggggtgaga
     2761 gggaaacaat ttttattcct cgactaaaat tgagcgatga cgttttctat tctgaaaaac
     2821 agactggcag gaacttcatc attacgccca tttattctga ttaagttctc cagatttctc
     2881 tctaactcgt acatctggca gcatttaaag caccacttca gatgttcttc gatttgccgc
     2941 ctttcgcttt cggttaatat ttcattgtct aaatacagat atatcttacc taagacttct
     3001 ttgcagtcca tccttcctgg ttcttccttt ctgaatcatt tctccgggtg gaagagtatt
     3061 cccatagcga cttttgcagc aatcttctac ctcgccacag acgagacata actgtcccta
     3121 taggtatatc cagtatttct gctgcttctt tataggagaa gccctccacc aggaccaaga
     3181 ccaccacagc tctaaactct acaggtagat cctctatagc tttaagaata tcctccttgg
     3241 ttaaagtatt taacaaagct tcctctgggg ttattccctc agctataggt tggaaaaaag
     3301 attcttcttc catctcatct aaggaaggaa gcttttgcct gtctcttacc cggtaatagt
     3361 tgtttataaa agtgttggtt aagattcgga atatccaggc tcgaaaatta gtcccctttt
     3421 caaaccgatc aaaggcagca aaagctttgg tcactgtttc ttgaactaaa tcttcagcgt
     3481 cttctcggtt acgagtcata cgcagagcag tacgaaacag cgcccctaat tcaggattca
     3541 ataactcttg aaaatatttc ttttcttctt ctttggtact caccattacc aatcaacctt
     3601 ttcaggaatc ctttaaaaaa tcagcatttt ttaaattttc cagctctttg tgaaactgga
     3661 ttatttcgtc aatggttatt tggcctttgg gtttggcctt tttcttcttt ttagtggtaa
     3721 tccgtgctac atcctccagt ttctctaagt taaaattaac ttcgcagaaa tctttgcaat
     3781 tgggacatat atatcttatg tatccactgc cccggtaagc aggaatttta taaattccaa
     3841 tttctgttac gtcctccaag gtaatcaacg ccttacaatt tttgcacttt aaaggaagct
     3901 cctgcatttc ttcctcctaa actatttttg aatattttac caatatatag taaataatac
     3961 aaggaaaatt ttttctggcc tctgaacata gctttatata acccgttaca caatttccct
     4021 gaatatttgc cggccttttt ctccctcaac gttgaagtgt ttttcctcca gcgtacgatg
     4081 tgacggggct tgcaatagca ggctgaggtg gattatcctc aggaaaaagg tacaaaaacc
     4141 ccgcaattta gctctcttgc aactctcaag aagaagtacc acctgagggg gcttcctctc
     4201 aggggtggta tttgatagaa taggttacac ttcctgatta taaaaagagg ggggtattgt
     4261 tgagaataaa agttaccttt cagtccgacc gtaaaatatg tcttcccact catcacaacc
     4321 acatcattca gggcttcata tattcaaatc tggatcgggc tctctccaat atgcttcaca
     4381 gtgagggata caagctgggc tcaagaacct ttaagctctt cactttttcc cgacttctgg
     4441 gaaaaatgaa ctataaccct caagaaggat tcactttcta tcctcctgtt tctctcctta
     4501 ttgcctctcc caaggttgat attttagaaa gcctatctca ctttttagtg aaaaggaggg
     4561 aactgagttt aggaaataat aaagtctgga cggaaagcgt cgaagtcatg atggagaaaa
     4621 agtttaccaa gagagtgcag gttgaaatgc tctcgcctgt cgtaacctat agcacactat
     4681 atacccagga aggaaagaaa aagacctatt actacaaccc ccaggaaaaa gaatttggag
     4741 aaaagattcg agataatctg ataaaaaaat acctcttagt taatgactca cccctcgatg
     4801 ataatcttca gtttgatatt gtccctctcc gggtgaataa aaatcatgag aaaatcattg
     4861 tttataaaca aactgtcatc cacgggtgga tgggacaata cgagataacc ggaaaccctg
     4921 agcttatcaa ggtgagctat gatgctggct tggggagtaa aaactcctcg ggatttggta
     4981 tgtgggaagt gtggagagag gattttccgg agcaaagaat accataagcc aggaaagaaa
     5041 taacaagcca cctatgaggg ggaagactat aagagggtaa acccccttat aaaccccgag
     5101 aaaagcaaaa acaaaagata aaaaataaag atttactgag gggaaagctt cctcagtaaa
     5161 agcttttgga ttcccgataa aagatttcgg gaatgacaga aagtactata agggggcgtt
     5221 gcccccttat aaacccccgg aaaagacgaa aaca
//
LOCUS       DAOPSS010000110         5250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12777_length_5250_cov_9.538402, whole genome shotgun sequence.
ACCESSION   DAOPSS010000110 DAOPSS010000000
VERSION     DAOPSS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12777_length_5250_cov_9.538402"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..2496
                     /locus_tag="PKN31_06645"
     CDS             94..2496
                     /locus_tag="PKN31_06645"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOA99637.1"
                     /translation="MEEKKEKTNHNLTIPILGMKCAACASRVEKGLEAIPGIHSVDVN
                     LALSKATINYDSKKTNFQDIINEISKIGYEVPEEEIELLIEGMSCASCATRIEKKLNT
                     LAGVKKAAVNLASNKAYIQFVPGIINQAEIEEAIESLGYGVQTTSRILSEKRKGRKQN
                     RENWQKIRFFVALFLSLPLFYMMVANWTGKGQFIIDPWIQLLLATPVQFFSGWPFYRG
                     AYYSLKSGSASMDVLVALGTSVAYFYSLISLVAGWGVFYFESSAMLITIILLGKLLEE
                     RAKWKASEAVEKLVKLQPQTAHVLQNGIEKDIPAERIKVGDMVAVKPGECIPVDGVIV
                     EGSSTVDESLLTGESMPVEKHPGCEVIGGSINQEGSFIFRATKVGADMVLARIIKMVE
                     EAQGSKAPLERLADRVSAVFVPVIIVLSVITFLGWYIEGAGFASSLMHMVTVLVVACP
                     CALGLATPTAVMVGIGLGAEKGIFFRGGEYLEGIEKVDTVLLDKTGTITWGTPSVDDF
                     FVPSSFDINETWAIISSGEKWSEHPLGQAIAKYGEKLDISPNGKIKNFNALPGKGVRF
                     QYQDEVWYIGKEELLESQGIDLMEVAEYKSRWEREGKTVVVAASGGKVRAIIALTDTI
                     KDEARKVISELKQMGLEVVMVTGDQEVVAQAIAQKAGIDKVFSGVMPDEKVEIVKKLK
                     EEGRTVAMVGDGINDAPALAQADVGIAIGTGTDIAIESADVILMNGELKSLLSALRLS
                     RFTRRKIKQNLIWAFIYNLVTVPLAMFGIFTPIMGGVAMALSSTSVVANSLLLKRYRN
                     KI"
     gene            2663..3118
                     /locus_tag="PKN31_06650"
     CDS             2663..3118
                     /locus_tag="PKN31_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOA99638.1"
                     /translation="MDKNRGVMSNFFRSWRLRWHIVSLEKEKEESLLRLGKRVYELFI
                     ERKDWGREEVRENCQEVLSIEEEIEDLEEKMRRLEGNKPRRICPSCGEYIEEGSLYCS
                     RCGFHQKQQELTCSQCGEKINPQDKFCPHCGQEIKQPSSHKTEEEEKND"
     gene            3111..4412
                     /gene="purB"
                     /locus_tag="PKN31_06655"
     CDS             3111..4412
                     /gene="purB"
                     /locus_tag="PKN31_06655"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006323587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOA99639.1"
                     /translation="MIERYTLPEMKKIWSDEHRFEIWLKIELLALEAQQKLGLLPQED
                     LEKIKKGISFSPDRMQEIEKVTRHDVIAFLTTLSENLGPESRFIHFGMTSSDVLDTAN
                     AVLMKEALDLVLKDMDGVLDSIKNKALQYRYTLTVGRTHGVHAEPTTFGLKMAVWYAE
                     MLRNKERLEKAKENIRVGKVSGAVGNFAHFDPRVEEYVCEKLGLKPDPVSTQIVQRDR
                     YAEVIWALAMVGGSLEKFAVELRNLQRTEIREVEEGFSEGQKGSSAMPHKKNPITGEQ
                     ICGLSRILRGNLVAALENIPLWHERDISHSSVERIILPDCFILTDYLLHTFQRLLDNL
                     VVYPENMRKNLEKSRGLVFSEKILLELIKKGLTREDAYALVQHCAMKTWEDESLSFIE
                     VLKNDSSIREYFSLEELESFFDYNYFLRQVDFILKRAGIEG"
     gene            4416..>5250
                     /gene="purM"
                     /locus_tag="PKN31_06660"
     CDS             4416..>5250
                     /gene="purM"
                     /locus_tag="PKN31_06660"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOA99640.1"
                     /translation="MEERWNYKKAGVDIDLGDKLIQDIKDLVSHTYRPEVISGVGGFS
                     GVFRLGEKWKEPCLVAGSDGVGTKLKIAIDLDKHDTVGIDLVAMCVNDVLCAGAEPIF
                     FLDYLACGKLEAEKFKSIISGIVRGCEEAGCALLGGETAEMPDFYKEGDYDLAGFAVG
                     VVEKDALIDGGKIRKGDVLLGLASSGLHSNGYSLVRKVLQSRGINLSDQINGKILGEE
                     LLRPTRIYVRSVLPLFREVEVKGVSHITGGGMIENIPRILLPHLGVKIYKSKIEIPWV
                     FS"
BASE COUNT         1616 a          829 c         1279 g         1526 t
ORIGIN      
        1 caaatcttgt ctggtgatga gagtgctatt gatgaagtcc tggccactat tttcagaatg
       61 atgagataag gctcaaatag ggaggcccta aagatggaag aaaagaaaga gaaaaccaac
      121 cacaatttga ccattcccat tttgggaatg aaatgtgcag cctgtgcttc gcgagtagaa
      181 aaaggactgg aagctattcc aggaattcat tcagtggacg ttaatctggc tttgagtaaa
      241 gcgacaataa attatgactc taaaaaaact aattttcagg atataatcaa tgaaatatca
      301 aaaatcggtt acgaagtgcc agaagaagaa atagagctct tgattgaagg gatgtcttgt
      361 gcttcttgcg cgacaagaat tgagaaaaaa cttaacaccc ttgcgggagt taaaaaagca
      421 gcggtaaact tagcttctaa taaagcctat attcagtttg tacccggaat aattaatcag
      481 gctgagatag aagaagccat agaatctctg ggctatggtg ttcaaaccac ttctcgtatc
      541 ctttcagaaa aaagaaaagg ccgaaagcaa aatagagaaa actggcaaaa aatcagattt
      601 tttgtcgctc tttttttatc acttcctctt ttttatatga tggtagccaa ttggacggga
      661 aaaggccaat ttatcattga tccctggatt cagctccttc ttgccactcc ggtgcagttt
      721 ttttccggct ggccatttta ccggggagct tactattctt tgaaatcagg tagtgccagt
      781 atggatgtcc ttgtagcttt gggaacttcg gtagcttatt tctatagtct gatttcttta
      841 gtagcgggct ggggggtgtt ttattttgaa tcctctgcca tgttgattac tataatttta
      901 ttgggaaaac tgctggaaga gagagccaaa tggaaagcct ctgaggctgt ggaaaaactg
      961 gtaaagttgc aaccccagac tgcccatgtt cttcaaaatg gaatagaaaa agatatcccg
     1021 gcagaaagga taaaggtggg agatatggtg gcagtgaaac ccggtgagtg catcccagta
     1081 gatggagtaa ttgtagaggg aagctctacc gtagatgaat ctttgcttac cggcgagagt
     1141 atgccggtgg aaaaacatcc tggttgcgaa gttataggag gctctattaa ccaggaagga
     1201 agttttattt tccgggctac taaagtagga gccgatatgg tgttagctcg tatcattaaa
     1261 atggtagaag aagctcaagg ctctaaggca ccccttgagc gtttagctga ccgggtttct
     1321 gctgtatttg ttcccgtaat tatcgttctt tcggttatta cttttttggg ctggtatata
     1381 gaaggtgctg gctttgctag ttctctcatg catatggtta ccgttttagt ggtagcttgt
     1441 ccctgtgctt tgggtttagc tactcccact gcagttatgg ttggaattgg tttgggagca
     1501 gaaaagggaa tttttttccg ggggggagaa tatttagagg gtatagaaaa agtcgatacc
     1561 gttcttttag acaaaaccgg gaccatcacc tggggaactc cctcagttga tgattttttt
     1621 gtcccttctt cttttgacat aaatgaaacc tgggcgataa tctcctcggg agagaagtgg
     1681 tctgagcacc ctttggggca ggccatagct aaatatggag aaaaacttga tatatctcct
     1741 aacgggaaaa ttaaaaattt taatgctctc ccgggaaaag gagttcgttt tcaataccag
     1801 gatgaggtct ggtacatagg gaaagaggaa cttttggagt ctcaagggat agatttaatg
     1861 gaggtcgcag agtataaaag ccgatgggag agagaaggta aaactgtagt ggtggctgca
     1921 tcggggggaa aggtaagagc gattattgcc cttaccgata ccataaagga tgaggctcga
     1981 aaagttatct ctgaactaaa acaaatgggg ttagaggtgg taatggtaac tggtgaccaa
     2041 gaagtagtag cccaagccat agcccaaaaa gcaggaattg ataaagtttt ttctggagtc
     2101 atgcccgatg aaaaagtaga gatagttaaa aaattaaaag aagagggaag aacagtagcc
     2161 atggtggggg atggcataaa cgatgctcca gctctggccc aggccgatgt aggaatagcc
     2221 ataggtacgg gaactgatat cgctatagag agtgctgatg taattttgat gaatggtgag
     2281 ctgaaaagtt tactctctgc tttacgtctt tctcggttta cccgaagaaa gataaaacag
     2341 aatttaatct gggcgtttat ttataattta gttactgtgc cgttagctat gtttggtatt
     2401 tttactccta ttatgggagg agtagctatg gctctcagtt ctacatctgt agttgctaat
     2461 tctcttttac ttaagcggta caggaataaa atttaggcga acttttaacg cttctatcgc
     2521 tttgatattt gaattatgga atagccatct taaaatgatg ttattgtaga aataaaaatg
     2581 acctttcttt atcttgtaaa atatataaga tgaatcatct ctatgttaaa attgaggtaa
     2641 attcaggagg accaagttat aaatggataa aaatagagga gtaatgtcca atttcttccg
     2701 ttcttggcgt ctgcgctggc atatagtctc tttggagaaa gaaaaagaag aatctcttct
     2761 tcggctgggg aagagagttt atgaactttt tatagagcgg aaagattggg ggagggaaga
     2821 agtgagagaa aattgtcagg aagtgctctc tattgaagag gaaattgaag atttagaaga
     2881 aaaaatgcgc cgtttagaag gaaataaacc ccgccggatt tgcccttctt gtggagagta
     2941 tatagaggaa ggttctcttt attgctcccg ctgtggtttt catcaaaagc agcaggaatt
     3001 aacttgctct caatgtggag agaaaataaa ccctcaagac aaattctgcc ctcactgtgg
     3061 ccaggaaatt aaacaacctt cttctcataa aacagaggag gaagaaaaaa atgattgaaa
     3121 gatacaccct tccagagatg aaaaaaatct ggagtgatga acaccgcttc gaaatctggt
     3181 taaaaataga gcttttagct ttagaggctc agcaaaaact aggacttcta cctcaagaag
     3241 atttagaaaa aattaaaaaa ggtatttcct tttcccccga taggatgcaa gagatagaaa
     3301 aagtaactcg ccacgatgtc atcgcttttc tcactacctt atcggaaaat ttaggacctg
     3361 agtctcggtt tattcatttt ggtatgactt cttccgatgt tttagatact gctaatgctg
     3421 ttttgatgaa ggaagcttta gatttagtgc taaaggatat ggatggggta ttggattcta
     3481 ttaaaaataa agctttacaa tatcgttaca ctttaacagt gggaagaact cacggagttc
     3541 atgccgaacc taccactttt gggctaaaga tggcagtatg gtatgcagaa atgctccgta
     3601 ataaggaaag attagaaaaa gctaaggaaa atattagagt gggtaaagtt tctggagcgg
     3661 tgggaaattt tgctcatttt gaccctcggg tagaagaata tgtatgtgag aaactgggcc
     3721 taaaacctga tcctgtttct actcaaattg tccaaagaga ccgctatgct gaggtaatct
     3781 gggctttagc tatggtgggg ggaagtttag aaaaatttgc tgtggagctg cgtaatctcc
     3841 agagaacaga aattagagaa gtagaagaag gcttcagtga agggcaaaag gggtcatctg
     3901 ctatgcccca taagaaaaat cctattactg gagaacagat ttgtggatta agccggattt
     3961 tacgaggcaa tttagtggca gctttagaaa atattcctct ctggcatgaa cgagatattt
     4021 ctcactcttc agtagaaagg ataatactcc ctgattgctt cattttaact gattatctac
     4081 ttcatacttt tcagcgttta ttggataatc tggtagttta cccggaaaat atgagaaaaa
     4141 atctggagaa aagtcgaggt ttggtttttt cagagaaaat acttctggag ctaatcaaaa
     4201 aaggattaac tcgagaagat gcttatgctt tagtgcaaca ctgtgccatg aagacctggg
     4261 aagacgagag cctatctttt atcgaggttc tcaaaaacga ctcttcgatt cgagagtact
     4321 tttctttgga agaactggaa tcttttttcg attacaatta ttttcttcgc caggtagatt
     4381 ttattttgaa aagggcggga atagagggct aaataatgga agagagatgg aattataaga
     4441 aagcaggagt agatatcgat ctgggagata agctaataca agatataaaa gatttagttt
     4501 ctcacactta tagacctgaa gtcatttctg gagtaggggg gttttcgggg gtttttcggc
     4561 tgggagaaaa atggaaagaa ccttgtttgg tagcgggaag cgatggagta ggtactaaac
     4621 taaaaatagc catagatttg gataaacatg atactgtagg catagatctc gtagctatgt
     4681 gtgttaacga tgtcttatgt gccggggctg aaccaatatt ttttctcgac tatttagcgt
     4741 gtggaaaatt ggaagcagaa aagtttaaaa gcataatatc gggaattgtc aggggatgtg
     4801 aggaggcagg gtgtgctctt ttgggaggag aaacggcaga aatgcccgat ttttataaag
     4861 aaggtgacta tgacctggcg ggttttgctg taggagtggt agaaaaagat gctttaattg
     4921 atggagggaa aattcgaaaa ggggatgttc tcttgggatt agcttcttcg ggtctgcata
     4981 gtaatggcta ctctctggtg agaaaagttt tacaatctcg ggggataaat ctttctgatc
     5041 agattaacgg taaaatttta ggagaggaat tacttcgtcc taccagaatt tacgtgagat
     5101 cagttctccc tctttttcgg gaagtagagg taaagggagt gtctcatatt actggaggag
     5161 gaatgataga gaatattcct cgaattctcc ttcctcatct gggagttaaa atttataaat
     5221 ctaaaattga aattccctgg gttttttctt
//
LOCUS       DAOPSS010000111         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12840_length_5228_cov_8.263677, whole genome shotgun sequence.
ACCESSION   DAOPSS010000111 DAOPSS010000000
VERSION     DAOPSS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12840_length_5228_cov_8.263677"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            608..919
                     /locus_tag="PKN31_06665"
     CDS             608..919
                     /locus_tag="PKN31_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99641.1"
                     /translation="MNKKDSTTKRNLLIAGLVTIVLGIIQVLVFPPLIIGVGFVRSLI
                     PFIVSCIFLILGWKKMWLLLIPGAFLLGSLLGVVFNYPYGGALLGAGVGLLISAFPRE
                     E"
     gene            complement(957..3143)
                     /locus_tag="PKN31_06670"
     CDS             complement(957..3143)
                     /locus_tag="PKN31_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOA99642.1"
                     /translation="MFKFKSLRSRLLFWFLILSLIPLLTLAILGITEFQDTLLDQEIE
                     NDKVIALEMGKTLSIMMDEGLGHAHFLTQNYYIMNPDSDVAEKSAVLKDFVSNHTVYA
                     TASLVDSQGIQIADSHDNGIGEDKSSTEWFQAVNSTKKSYLSDLRESIDLGVYIMNFA
                     APVFDEDDNFVGVITIRMDIQKTSEELIRGIQLAETGYVYVYDVSQNKVVIHPDFNIV
                     GKTLEELDLEFLKEVFQEGEEGTHRYTFQGVDKIVTFHKIEPWGFFSGDNLKNWQVVA
                     TFPYDEIVAPARGMAKLIFIIVVITAIIVVFVSRAVAYSLARPLEKITAVAQKVAEGD
                     LAQEVEEIKTQDEIGRLSAAFRGMLEYIRELVTNIINTSSTLAASSEELSSSIEEVSK
                     ATEEIAKTISQIAQGSTEQSVEIEKVNRGAEEIVQKVDAVNQSTAKNLELLQEMQKAL
                     SLNYESLSSTHKVMADTAQESEENRQVAQKGEELLSTLEDNIRSISQATKEVAQSIAT
                     LDERSQEVGKIVDLISGIAEQTNLLALNAAIEAARAGDAGRGFAVVAEEVRKLAGEAG
                     EAAKNIATLVREIQNDTKEVVEKMKGAEERVEEGVAQGEETSKNFFQIRTSAESIIES
                     VSRLSSTLEEAEESLHRAIESAQEVSALSEDNSQLIDQVNQSIRSITEEISSIASVSE
                     ENAASSQEVSASTEEQSASLEEISSATESLAKLAQELQKLVEKFRV"
     gene            3386..3472
                     /locus_tag="PKN31_06675"
     tRNA            3386..3472
                     /locus_tag="PKN31_06675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3420..3422,aa:Leu,seq:cag)
     gene            3580..3816
                     /locus_tag="PKN31_06680"
     CDS             3580..3816
                     /locus_tag="PKN31_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99643.1"
                     /translation="MGEVVKLPQPDDWKNLLELFLFEKKVEGRAPRTISDYKLHATIF
                     FKCFPRALRSEKKLKACILKYFSDDVKPVTSNFR"
     gene            4211..5002
                     /locus_tag="PKN31_06685"
     CDS             4211..5002
                     /locus_tag="PKN31_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOA99644.1"
                     /translation="MDLGIEGKIALVTGSSQGMGKAIARELSREGARVILSSRSSTRL
                     EEAQKEIEKETGGECLFFPCDLSNGEDIKNLVNYVTREMQGVDILVINSGGPPPGNIF
                     SISEEDWYKSFDLLLMSAIRLTKAFLPYMEAKNWGRITYLTSTSIKEPIENLLLSNVL
                     RSGVSALSKSLSRDYAHRNVLFNVVCPGSIATRRTIQLLEDRAARSGQPLEILRQEAE
                     QAIPMKRYGNPEEVASLVVFLCSNQATYLTGTAIQVDGGAIRFVL"
BASE COUNT         1568 a         1125 c         1012 g         1523 t
ORIGIN      
        1 gacaaagaac taaccgagga agaaaaagca tcttcctcgt taaaagcctt tggatgcctg
       61 ataaaaaatt tcaggcatga cagaacagaa ctataagggg acgccgcccc ctttatcaac
      121 ctccaaaaaa agaaaaagac caaaaacaac acagaggaaa aaatctctgt gtgtcactcc
      181 tgaatgcttt tatcgactat aagtggtttc acccccttat aacccacgaa aggcaagaac
      241 taaaaactta aataaaaaga ataaatccac taaaggagaa aaagcatccc tcagtgggtt
      301 cctcgctaaa ttttgggaat agcaaaaaaa actaaggctt ctcaccttct cgggctttcc
      361 ctcttatcag ggcgggataa tcgaacataa aatagtcttt aaaaagccaa cctacccctt
      421 ataagataaa gacgcactga gtagaatgtg agttacaatg ggttttgtga ttaactataa
      481 ccatccttaa aacaaataca gaagaaaaat gaagctggaa aagagcaaaa cttgagaaaa
      541 agaaagcagc acagacaatt atattttgat aaaatatgtg ttaaacaaca aataaggagg
      601 gagcactatg aataaaaaag actctaccac aaagaggaat cttctcattg ccggactggt
      661 aaccattgtt ctggggataa ttcaggtgtt agtttttccc cctcttataa taggagtggg
      721 gtttgttcgc tctctcattc ccttcattgt tagttgcatt ttcctcattt tgggctggaa
      781 aaagatgtgg cttctcctta tacctggtgc ttttctctta gggtcattgt tgggagtggt
      841 tttcaactat ccttatggag gagctctttt gggagccggg gtgggacttt taatcagtgc
      901 tttcccgaga gaggaataat ttctggaggg gggcattgcc cccctcatca tctatctcaa
      961 actctgaatt tttccactaa tttctgtagt tcttgagcca gtttagccag agactcagta
     1021 gccgaagaaa tctcctccag tgatgcactt tgctcctcgg tagaagcaga aacctcctgt
     1081 gaagaggcag cattttcctc tgaaaccgag gcaatagagg aaatctcctc agtaatagac
     1141 ctgatgctct gattaacttg gtctatcaat tgagagttat cttcagaaag cgcactcact
     1201 tcttgcgcac tttctatagc cctatgtaat gattcctccg cttcttctaa agtagaggaa
     1261 agacgagaaa cgctttctat tatactctca gctgaagtcc taatctggaa gaagtttttg
     1321 ctggtctcct ccccttgagc tactccttct tccactcttt cctctgctcc tttcatcttt
     1381 tctaccacct ctttagtatc attctggatt tcccgcacta aagtggctat gttcttagct
     1441 gcttctcctg cttctccagc taattttctc acttcttctg caaccactgc aaagccgcgc
     1501 cctgcgtctc ctgctcgggc tgcctcaatc gctgcattta aagctaagag gttagtttgt
     1561 tcggcaatcc cggatatgag gtctacaatc ttacctactt cttgagaacg ctcatctaaa
     1621 gtagcaatgg attgagccac ttctttggtg gcctgagaaa tagagcgaat attatcttca
     1681 agagtagaaa gcaattcctc acctttctgg gctacttgac gattctcttc tgactcttga
     1741 gcagtgtcag ccattacttt atgagtagaa gacagagatt catagttaag agaaagagcc
     1801 ttctgcattt cctgcaaaag ctctaaattc ttcgctgtag attggttgac agcatctacc
     1861 ttttgtacga tctcttctgc ccctctattc accttctcaa tctctacact ttgttcagta
     1921 gaaccttgag caatctggga aatggtcttg gcaatttctt cagtagcttt ggaaacctcc
     1981 tcaatagagg aggaaagctc ctctgaagaa gcagcaagag tagaagaggt attgatgatg
     2041 ttggtaacta actcccgaat atactctaac ataccgcgaa aggcagcact caatctccct
     2101 atctcatcct gagtttttat ttcctctact tcctgagcca aatctccttc tgccactttt
     2161 tgagctacag cagtaatttt ttccagagga cgagccagag aataagcaac agcccgagac
     2221 acaaagacaa caattatagc ggttatcacc actataataa agattaattt agccattcct
     2281 cttgctggag caacgatttc atcatagggg aaagtagcga ctacctgcca atttttaaga
     2341 ttatccccgg aaaagaaacc ccagggctca attttgtgaa aagtaactat tttatctacc
     2401 ccctggaaag tataacgatg agtgccttct tctccttctt gaaatacctc cttcaaaaat
     2461 tccaggtcca attcctccag tgtcttaccc acaatgttaa aatcgggatg aattaccact
     2521 ttattctgag acacatcata cacgtagaca tatccagtct cggcgagctg tatccctcgt
     2581 atgagttctt cagaggtttt ttgaatatcc atacgaatag ttattactcc cacaaaatta
     2641 tcgtcttcat caaaaaccgg tgcagcaaaa ttcataatat atactccgag gtctatagat
     2701 tccctgaggt cacttaaata agacttttta gtagaattaa ctgcctgaaa ccactcagta
     2761 gagcttttat cctctccgat accattatca tgagagtcag ctatttgaat gccctgggaa
     2821 tcaacaaggg aagcggtagc ataaacagta tggttagaaa caaaatcctt taaaacagca
     2881 gacttttcag caacgtcaga atcgggattc ataatatagt aattttgagt taaaaaatga
     2941 gcatgtccta acccctcatc catcattatg gaaagtgttt ttcccatctc aagagcaatt
     3001 actttatcat tttcaatctc ctggtctaaa agagtgtctt gaaattcggt aattcccaaa
     3061 atagctaaag ttaaaagagg gattaaagaa aggattaaaa accaaaaaag caatctactc
     3121 cgcaggcttt taaatttgaa cattttactt cctcccctat tttcgcgatt tagttctgca
     3181 acccacccat aaataagtat cggcaaaaat gggaaaaact ttagcttgga aaagaagtca
     3241 tctagaatgc ctttcagctt gatctgtgaa tcttctatat cgctcccgtt atttttttct
     3301 actgttatta tcatagaata accatgtctg aaagcctctg taacccttga caagtgagga
     3361 aatttgagat attttactat gggttgccga agtggcggaa atggcagacg cgctggaatc
     3421 aggatccagt gagtgaaagc tcatgcgggt tcaactcccg ccttcggcac caggtgcgct
     3481 atagagctct tttagcctct tctataattt tactctcccc tctctgaaag cacgagtcca
     3541 gtggctataa aatcgactcc cagagagagg tgctttttta tgggagaagt agtaaaactt
     3601 ccacaacctg acgactggaa aaacctttta gagctatttc tgtttgaaaa gaaagtagaa
     3661 ggaagagctc cccgcactat ttccgattat aagctccacg ctacaatatt cttcaaatgc
     3721 tttcctcgtg ctttacgttc agagaaaaag cttaaggctt gcattcttaa atacttctcc
     3781 gatgatgtta aacctgtcac ttcgaacttc cgctaacagt attttagagc tttcttcagc
     3841 tttttagggg gtgaaaatgc catcgagaaa acctcgttgg ttttaaaaaa agagaagtgt
     3901 tttagccttg tgatgcttct ccaaacccac catcgcgaat gctgaggaaa aagactttct
     3961 cctcagtgat ttttctatat tttttgtctt ttttcacttt tcagggggtt atgagaggga
     4021 taaatccctg aaaagttctt tcccgtcatt ctcgaaatca gtagtcagga attcagaagc
     4081 tttgcgagat aacaccacaa aaacactctg gcaggcagtg ggggcccagt ggccagaagc
     4141 cagagctcac agtatttatc gtcatattag attaatgata caatttagca aatttacgag
     4201 gaggaaaaag atggatttag gaatagaagg taagatagct ttagtaactg gaagcagtca
     4261 ggggatgggt aaggccatcg cccgggagtt atctcgagaa ggagctcgag ttattctttc
     4321 ttcccgttct tcaaccaggt tagaggaagc ccaaaaagag atcgaaaaag aaacgggtgg
     4381 ggaatgctta tttttcccct gtgatttaag caatggggaa gacataaaaa atctggtgaa
     4441 ttatgtaacc agagaaatgc aaggagtgga cattttagta ataaatagcg gaggtccccc
     4501 tccaggaaat attttctcta ttagtgagga agattggtat aaatcctttg atttactttt
     4561 aatgagtgcc attcgattaa ctaaagcctt ccttccttat atggaggcta aaaattgggg
     4621 aaggataact tatctaactt ctacttctat caaagaaccg atagaaaacc tcttgctttc
     4681 taacgtctta agaagcgggg tcagtgcttt atctaaaagt ctatcacgag actatgctca
     4741 tcgaaatgtt ctctttaacg tagtttgtcc gggctctatt gctactcgca gaactattca
     4801 gctattagaa gacagagctg ctcgcagcgg tcaacctttg gaaatcttaa gacaagaagc
     4861 cgaacaagct attcctatga agaggtatgg aaatccggaa gaggtagctt ctttagttgt
     4921 atttttatgc tccaaccaag ccacctactt aaccggtacg gccattcagg ttgatggagg
     4981 agctattcgt tttgttttat aaactggaga aagtgaatcc atctcccctg atacgggaat
     5041 atcagattta gccgccgtaa aaaacaaaaa tcataagttg atggtatctt caagaggggt
     5101 tacagaagag ccttacctcc tatagttttc gcggctataa aaattaaata aaaaagccat
     5161 cggctccgac aagctttagc ctttgctttc tcgcatttag cgagaaattt caggatggac
     5221 ttccactc
//
LOCUS       DAOPSS010000112         5222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_12861_length_5222_cov_7.554867, whole genome shotgun sequence.
ACCESSION   DAOPSS010000112 DAOPSS010000000
VERSION     DAOPSS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12861_length_5222_cov_7.554867"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..450
                     /locus_tag="PKN31_06690"
     CDS             289..450
                     /locus_tag="PKN31_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99645.1"
                     /translation="MGIVTQPKGGARVNKVKDVSIDELEYLIEQKVLEILWDFDSRID
                     AHAARSTTI"
     gene            896..1108
                     /locus_tag="PKN31_06695"
     CDS             896..1108
                     /locus_tag="PKN31_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99646.1"
                     /translation="MERSSIDYGEAEILVLVLEKKTGLVLLNEKEVREVAEKLGFRVL
                     GTVGLLIGAKQEELYYLKWYTNYYET"
     gene            1098..1442
                     /locus_tag="PKN31_06700"
     CDS             1098..1442
                     /locus_tag="PKN31_06700"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOA99647.1"
                     /translation="MKLDENLKNQLIERLKPLNPEKVILFGSYAYGEPSKDSDIDLIV
                     VTDDDIMPSNFDEKMQFYLKVSNVLTDIQREIPIDLIVHTKPMHKRFIELNSMFYREI
                     AEKGIVIYERNN"
     gene            1426..1809
                     /locus_tag="PKN31_06705"
     CDS             1426..1809
                     /locus_tag="PKN31_06705"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOA99648.1"
                     /translation="MKEITEEWLERAYDDIETMGEIIDNPRLTNIVALHSQQAIEKSL
                     KAVIEELEIGAIKTHNLEKLFATVDDFIQFEVDWDLVKKLDALYIDARYPSEWGILSY
                     GKPTWKMQENFMNWQWKYTIWSRIF"
     gene            2552..4132
                     /gene="groL"
                     /locus_tag="PKN31_06710"
     CDS             2552..4132
                     /gene="groL"
                     /locus_tag="PKN31_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049018.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOA99649.1"
                     /translation="MAKEIIKDIKAREALGKGVDILANAVRVTLGPKGRNVLLEKKYG
                     LPVVTNDGVTIAQEIEVEESEKNVGVQVAKEIATKTNDLVGDGTTTALVLAQKMLREG
                     LRSIAFGHNPVLFRRGMEEALNRAIEKIKSYSLVVEDRKSISQVASISSNDPVVGEIV
                     AQALEKVGKDGVVTVEESKSAETTIEITEGMQFDQGYLSSYFVTDTERMEVVLEEPLL
                     LICDFKIANIISLLPLLQQVLRMDKPLLIIAEDIEQEVLATLVVNKLQGVLKVAAVKA
                     PAFGDRRKEILGDIAVLTGGQVISQDMGMRLEKVTVDLLGKVDKVRITKDNTTLIGGR
                     GNKKDIEARERQIRAQIEKTTSTYDREKLQERLARLQGGVAIIWVGASSEVELKEKKY
                     RVEDAINAAKAAIEEGIVPGGGALFVHIAQELDFSDLPEEERQGAEALRKALEAPAYW
                     IAENAGYDGSLVVEEIRKGDWRVGFEAQKGEFVDLITEGIIDPAKVLRVALSNAQSIV
                     SLILISEALIFEAPEEKI"
     gene            4187..>5222
                     /locus_tag="PKN31_06715"
     CDS             4187..>5222
                     /locus_tag="PKN31_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HOA99650.1"
                     /translation="MENWFRSDKGLLRTTYGFDDVSLVPGEITIDPQEVDITTTIGGE
                     KLDIPILGAAMDGVIDPRMAVEMGRLGGWGVLNLEGLFTRYDDPYSIIEEMVNQPREK
                     ITDFIQEVYRVPIKEHLVGERIQEIKREGVKAVASLTPSKAETLSKVAQEAGLDILVI
                     QSTVTTLRYHSSTSRVLDLEKFCRETPIPVILGNCATYKVALDLMKAGAAGILVGIGP
                     GAACTTRAVLGIGVPQVTATWDAARARDTYLKATGKYVAVITDGGMRVGGDIAKAIAS
                     GADAVMLGSPLASAQEAPGKGHHWGMATPDPNLPRGTLVKVGIRGTLKEILLGPSHLT
                     DGTMNLVGALR"
BASE COUNT         1567 a          911 c         1291 g         1453 t
ORIGIN      
        1 gtattaaagt atcgtgaccg ctcccagaag ggataaggag atcttcgtaa aaccatcacc
       61 acatccaggt cgctccccac tccccataaa gaggcgtaga gcttttaccg ctttttctgc
      121 tggcttcaat cagtcaaaag ctctccccac catagacgcc aatcctcttt ttaaatattg
      181 taatatagcc acaatttaat tcccttatgc ttttccctcc ctgtaagcca aaaaaagtag
      241 ctcattcccc tttcagactg gcgaaaagct aaagtaggtg atatcacaat gggtatagta
      301 actcaaccga aagggggtgc gagagtaaat aaggttaaag acgtgagtat agatgaactg
      361 gaatacttga tagagcaaaa ggttttagaa atactttggg attttgattc caggatagac
      421 gcccatgctg ctcgcagcac caccatatga ccatgaaaat acgaacgtct atcctgtatc
      481 cttttgttct tttatctttt aatcttacgc cgtaggctgt tatcctggtg tatttccagg
      541 atagaattaa cagatgagtt caagaagaaa ttagcgaaac gtctgaagaa gccatccggg
      601 agaatttccc ataaagaggt attagaggaa tttggccagg attgaatgga cggaagaagc
      661 ggtaaaggat ctcaagataa tcgaaaagca agtagggcga agggttacta agaaactcat
      721 ctggctttcc gaaaattttg aaacaattat ttctgaacta acctcaacac cttggaagag
      781 ttggattgac tgtggtctgc aattcttcga tattgattgt tttgagtgca attagtcggc
      841 tggatctgtt gagaaaacga tgtaagggtg cgaaaccagt aatccccatc gtagtatgga
      901 aagaagtagt atagattatg gagaggctga aatcctggtc ctggtactgg agaaaaagac
      961 gggcttggtt ctgcttaacg agaaagaggt aagagaagtt gccgaaaagt tgggtttccg
     1021 ggttttggga acggtgggtt tactcatagg ggcaaaacaa gaggaattat attatttaaa
     1081 gtggtatact aattactatg aaacttgatg aaaacctaaa aaatcaattg atagagaggc
     1141 tgaaacctct aaatcctgaa aaggtgatcc tttttggtag ttacgcctat ggagagccat
     1201 caaaagatag tgatattgat ttgattgtgg taaccgatga tgatattatg ccaagtaatt
     1261 tcgatgaaaa aatgcagttt tacttgaaag tctctaatgt tcttacagat atacaaagag
     1321 agatccctat tgaccttatc gtacacacaa aaccgatgca taaaagattt atagagctta
     1381 atagtatgtt ttaccgcgaa atagctgaga aaggaatcgt aatatatgaa agaaataact
     1441 gaggaatggc tcgaaagggc atatgatgat attgagacta tgggtgagat catagataat
     1501 cctcgcctga caaatattgt ggctcttcac tctcagcaag ctatagagaa atctctgaag
     1561 gcagttatag aggagctcga aataggagct attaaaactc ataatttgga aaaattgttt
     1621 gctaccgtag atgattttat acaatttgaa gttgattggg atttagttaa gaaacttgat
     1681 gcgctatata tcgatgctcg ctatccgtca gaatggggaa tattgtcata tggaaaaccg
     1741 acctggaaga tgcaagaaaa ttttatgaat tggcaatgga aatatacgat atggtcaaga
     1801 attttttgag gtcatatcca gaatgagaaa taacctgcaa aacggcattt actaatgctt
     1861 gatttctttc gctggagtgt ttttctccag cgtaattagt tttcttcacc tattttgctt
     1921 tcgtctttag cgccgcaggc acgctgggtg tttttctcca gcgttgtttt tccagattca
     1981 ccggtggagc ttttcccacc agtagtattt gctttttatc ttttgtcttt tacgcttttt
     2041 tgtttttcag ctttctcttt attttttggg gattatacag gggcaaaccc cctcatagtg
     2101 cttttctgtc atttccgaaa tcctttatcg gtaatccaag tttttgtttc ttctttttat
     2161 cgggaatcat aggagcgtct cctcccattg tttagattcc tgataaatac attcaggaat
     2221 gacacataaa agacgcattc ccttttcccg tcattccaaa atttagcggg gaatccactg
     2281 agggaggttt ttccttcctc agtaatcttt taatattacg ccgtaggctg tctgtttttt
     2341 ctccctcagt tgtcttttct ttttatttaa gtttttagtt tttgtctttt tgggggttat
     2401 agggaggttt cacctcctta tggttgatag agccattcag gagtgacaga aaaaaagtta
     2461 tgaggggagg ataaatcccc cttattgtct gttataatcc tttatgcgaa tgacttatat
     2521 atccatatat tacgcgagga aggtgaagta agtggccaaa gaaataataa aagatataaa
     2581 ggcaagagaa gctctgggta aaggagtgga tattttagcg aatgctgtta gagttaccct
     2641 gggtcccaaa ggtcgtaatg ttcttctgga gaagaaatac ggtttacccg ttgttacaaa
     2701 cgatggagtg accattgctc aggaaataga ggtggaagaa tcagagaaaa acgtaggggt
     2761 acaggtagct aaagaaatag ccactaaaac caacgatctc gtgggagatg gaactactac
     2821 tgctttagtt ttagcgcaga aaatgctccg ggaagggttg aggagcatag cttttggcca
     2881 caatccagtc cttttccgtc ggggaatgga agaagcacta aacagagcca tagaaaaaat
     2941 taaaagttat agcttagtgg tggaagatag aaaatctatt tctcaggtag cctctatctc
     3001 gtctaatgac ccggtagtgg gagaaatagt ggctcaggct ctggagaaag tagggaagga
     3061 cggggtggtc accgtggaag aatctaaatc ggctgagact accatcgaga taacggaagg
     3121 tatgcagttt gaccagggtt atctttcttc ttattttgtt accgatactg agcggatgga
     3181 agtggtttta gaagaacctc ttctgctcat ctgtgatttc aaaatagcta acattatttc
     3241 tcttcttcct cttttgcagc aagtgctcag gatggataag ccccttctta taattgccga
     3301 ggatatagaa caggaagtat tagctacttt agtagtgaat aaattgcagg gagtgctaaa
     3361 ggtggcagca gttaaagctc ccgcttttgg ggatagaaga aaggagattt tgggagatat
     3421 agccgtttta actggagggc aggtaatttc tcaagatatg ggcatgaggt tagaaaaggt
     3481 aaccgtagat ctattaggga aagtggataa ggtacggata actaaagata acaccacctt
     3541 aatcgggggg aggggaaata aaaaagacat cgaagcccga gaaaggcaga ttagagccca
     3601 gatagagaaa actacttcta cttatgatcg ggaaaagctg caagagaggc tggcaaggct
     3661 ccagggagga gtggctatta tctgggtggg tgcctcgagc gaagtagagc taaaggagaa
     3721 aaaatatcgg gtggaagatg ccataaacgc tgcaaaagca gcgatagagg aaggtatagt
     3781 tccgggagga ggagcgcttt ttgtgcacat agcgcaggaa ttagacttta gtgatttacc
     3841 agaagaagaa aggcaaggcg cagaagcctt aagaaaagct ctggaggcgc cagcctactg
     3901 gattgctgaa aatgcaggat atgacggctc tctggtggta gaagaaataa ggaagggcga
     3961 ctggagagtg ggttttgaag ctcaaaaggg agagtttgtt gacctcataa cagaaggaat
     4021 aatagacccg gccaaagttc tgcgagtggc attgtccaat gctcaaagta ttgtttcttt
     4081 gattttaatt tctgaagctc tgatatttga agcgccagaa gagaaaattt gagattaagc
     4141 aagggtaata gtataatttg gggaaaacgg gagaaaggtg ggtagaatgg aaaattggtt
     4201 tcgaagcgat aaaggtcttt tacgtactac ctacggattt gacgatgttt ctttggttcc
     4261 gggagaaatt accatagacc cgcaggaagt ggatataact actactattg gtggtgaaaa
     4321 attggatatc cccattttag gtgcagctat ggatggagtg atagatcctc gaatggcagt
     4381 ggagatgggg cgcctgggag gatggggagt tctaaactta gaggggctat tcacccgtta
     4441 cgacgatcct tattccatta tcgaggagat ggtaaatcag ccccgggaaa agattaccga
     4501 tttcattcaa gaagtttatc gagttcccat aaaggaacat ttagtgggag agagaatcca
     4561 ggagataaaa agagaaggag taaaagcagt agcctctctt accccttcta aagcagaaac
     4621 tttaagtaaa gtagcacagg aggccggttt agatatttta gtaatccagt ctaccgtaac
     4681 cactctccgt tatcactcct ctacctcccg agtgttagat ctggaaaaat tttgtcgaga
     4741 aactcccatt cctgttatcc tggggaactg tgccacttat aaagtggctt tagatttaat
     4801 gaaagcagga gcagcaggaa tattggtggg cataggtccc ggtgcggctt gtactacccg
     4861 ggcagtttta ggtataggcg tgccgcaagt taccgctacc tgggatgctg ctcgagcgcg
     4921 ggatacctat ttaaaagcca cgggcaaata tgtagcagtg attaccgatg gagggatgag
     4981 agtgggagga gatatagcta aagctatcgc cagtggcgca gacgctgtga tgctggggtc
     5041 tcctctggct tctgctcaag aggcgccagg aaaaggacat cactggggga tggctactcc
     5101 tgaccctaac cttcctcgag gaactctggt caaagtgggg ataaggggga ctttgaaaga
     5161 aattctcctc ggtcccagtc accttaccga tggtactatg aatttagtgg gagctttacg
     5221 gt
//
LOCUS       DAOPSS010000113         5177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_13000_length_5177_cov_9.475791, whole genome shotgun sequence.
ACCESSION   DAOPSS010000113 DAOPSS010000000
VERSION     DAOPSS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13000_length_5177_cov_9.475791"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1240)
                     /locus_tag="PKN31_06720"
     CDS             complement(119..1240)
                     /locus_tag="PKN31_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOA99651.1"
                     /translation="MKAREKTFTVALVICLIGFLLVPGGETQEIVALSLQEAVKLGLE
                     KSNEARLAKEKLEEARVTFEEGKATLLLSPSIIQELSLQNTWKIAQRNYEMTLTQHAL
                     SVEQGYYNVLKAQQSLDLAQNNLKRAESQYQNIQTKYSLGMVAEIDLLSAELELNRAE
                     NEIQKSRRALALAQFQFNQLIGNRENQNYQLSDKLDFQPLEVNLEESLKYALAHRLEI
                     EQAQDNVSLKEKEVEVNSNPYTPPLTLQKSKVQLSQAQINLENTQNSVILEVRQNYST
                     LKDAEELVPLQDQNSKIAQEKLRIAQAQFDAGLITTVDLLDKQYATYEAETAYLQAVF
                     DYRVAQATFFTSLGMSLEERPKTWQKESTEQPETQNQEG"
     gene            complement(1283..2689)
                     /locus_tag="PKN31_06725"
     CDS             complement(1283..2689)
                     /locus_tag="PKN31_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOA99652.1"
                     /translation="MKKSKTSLFPRGANLVVFLWLFSCFSGGLSQAGEIETINLSYVV
                     EKVLANSPEVLNARDAVETAKLNLSSSASQGLSPEVTLSGNWPIINGEDTSDMTWGIN
                     IKDAVYLFDKSPTEKTAQILLQEAESQLLLEEEAAKVQAVTYYLNILNGEKDYNIAQK
                     TLELYQILFEDQKKKWEEGNASSIDLLKAQENLENSKFLLQEKERELKLARENLNQLM
                     GVPLDTFFSLAPLSSPASLGRLDYSSLSLLLQERSSEKEALQREKERKEIQRKETKKA
                     TQPQISLAGEYKGEDFLTRLSLDREGIFSYEIQGGSSSYLSSGSYFSSSEGWGVGLEI
                     SWKIWDGKVTSNQLKATEIEIDQIEREVEALPQTLSLRVEEAVNQLLQAQSNRELTSL
                     VQKSAQDTYRVMEEQLKIGFITDRELLQSELQYQQAEANYQKAENNFWQAILNLLKTL
                     EEPIQIEENQIVIPSFLS"
     gene            complement(2721..3929)
                     /locus_tag="PKN31_06730"
     CDS             complement(2721..3929)
                     /locus_tag="PKN31_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99653.1"
                     /translation="MNLSETLQSAIASILSNKLRSFLTMLGIIIGVAAVVAMTSLGEG
                     TTEEIVGQIGSLGSNQITVIPGRSRGIPGMPVASRGGNMLEWEHYLQLQDFGFPGIKN
                     VIAENTTSLTVTHGRESIRGRVVGTTPQYPEVRNFKVDMGRFFNQKELDNAERVAVLG
                     RTVAQDLFSSPEEALGKKIRVGRITATVIGVMEEKSTGIQDLGEQVFIPITTMQKRLT
                     GSKYIQSMTIQTETEEDLDFVAQFLETFFTNRLKDPEKFSIINQQDILDTINQVTGTM
                     TLFLTAITGISLLVGGIGIMNIMLVSVTERTREIGLRKAIGARPFDILAQFVIESALL
                     SAIGGLIGIVMGIVGSQVISKFTSYPAIISVEAIIIAFSISVGIGLFFGIYPARRASQ
                     LDPITALRYE"
     gene            complement(3916..4668)
                     /locus_tag="PKN31_06735"
     CDS             complement(3916..4668)
                     /locus_tag="PKN31_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99654.1"
                     /translation="MNQPVIKVEDLWKIYQLGEVQVEALRGVSFEIEEGEFVAIMGPS
                     GSGKSTLMNILGCLDRPTRGRYFLDGQEVSQLNENQLAQIRNKKIGFIFQSFNLLPRL
                     TALQNVELPLIYAGIPSQKRKEQAIKSLEAVGLGDRIHHYPNQLSGGQQQRVAIARAL
                     VNDPSIILADEPTGNLDTRSSEEIMHILQELNQEGKTIVLVTHERDIASHTHRILHFR
                     DGQLVDNETIEQPVEALEVLKELVEEEVVESV"
     gene            complement(4686..>5177)
                     /locus_tag="PKN31_06740"
     CDS             complement(4686..>5177)
                     /locus_tag="PKN31_06740"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOA99655.1"
                     /translation="LSTMKVKILINEIDVPEVEKGQKAVVTLDAFPNQQFEGKVTTIA
                     PEGKIVENIVVFETTIELPNPDNILLSGMTAEAEIIIKSKDNAIIIPLEALEERGGKA
                     QVLVKGPDGQPVQKEVKTGLRSDTQVEIEEGLQEGEEVFLILSSTSFQSNFGPPMGMP
                     GGR"
BASE COUNT         1287 a         1121 c         1074 g         1695 t
ORIGIN      
        1 aatctctaag ttgcctttgc aagctcgtct ttttgcatgt gtggtaaccc tggattgcca
       61 gctctatgcc cttactgagg gggatggttt ccccctcagt agtcagtagt ttttgctttt
      121 aaccttcttg attttgtgtc tcaggttgtt ctgtgctctc cttttgccag gttttaggtc
      181 tttcttctaa gctcattcct aaagaggtaa agaaggtagc ttgagccacc cgataatcga
      241 atacggcctg gaggtaagca gtttcggctt cataagtagc gtattgcttg tctaaaaggt
      301 ccacggtagt tattaagcca gcatcaaatt gagcctgggc gatgcgcagt ttttcctggg
      361 caattttaga gttttgatcc tgtaggggaa ctaactcttc tgcgtctttc agggtggagt
      421 agttttgacg tacttctaaa attaccgaat tctgggtgtt ttccagattt atttgtgctt
      481 gagaaagctg gacttttgat ttctggagag taagaggcgg agtgtaggga ttgctattta
      541 cttctacctc tttttctttt aagctgacgt tgtcctgggc ttgttctatt tctaaccgat
      601 gagcaagggc atatttcagg ctttcttcca ggttcacctc taagggttga aaatccaatt
      661 tgtcggaaag ttggtaattt tgattttctc ggtttcctat tagctgatta aactgaaact
      721 gagctaaagc aagagctctt cgggatttct gaatttcatt ttcagctcga tttagctcca
      781 gctcagctga tagcaaatca atttctgcta ccattcccag agaatacttg gtctggatat
      841 tctggtattg gctttctgct ctctttagat tgttttgagc taaatctaaa ctttgctgag
      901 ctttaagaac attatagtat ccttgctcca cagatagagc gtgttgagtc agggtcattt
      961 cataatttct ctgggctatt ttccaggtgt tttgaagaga tagctcctgg ataatagaag
     1021 gagaaagtag aagggtagct ttcccttctt caaaagtgac tcgtgcctcc tccaatttct
     1081 ctttggctaa ccgggcttcg ttgctctttt ctaatcctaa ctttactgct tcctgtagag
     1141 aaagagcaac aatctcttgt gtttctcccc ccgggacaag caaaaaacct attaggcaaa
     1201 ttaccagtgc cacagtaaaa gtcttttctc tcgctttcaa attcccaccc cctaaatgat
     1261 aatatgatta aaccagttat ttttaagata gaaaagaagg aattactatt tggttttcct
     1321 ctatctggat tggttcctcc agggttttca agaggtttaa gatagcttgc cagaaattat
     1381 tttctgcttt ctggtagttg gcttctgctt gctggtattg aagctcgctt tgcagaagtt
     1441 cacggtcggt gatgaaccct atctttagct gttcttccat aaccctgtag gtgtcctggg
     1501 cgcttttctg aaccaacgag gttagttctc gattactctg ggcttgaaga agctggttca
     1561 cagcttcttc aactctgagg ctaagagttt gaggaagcgc ctccacttcc ctttctatct
     1621 ggtcgatttc tatttctgtt gctttcagtt gattgctggt aactttcccg tcccagattt
     1681 tccagctaat ttccagaccc actccccagc cttcagaaga ggaaaaataa ctaccgctac
     1741 tcaggtatga ggagcttcct ccctgaatct cgtaagagaa aattccctct cggtcaaggg
     1801 aaagccgggt tagaaagtct tcccctttgt attctcctgc tagagatatt tgaggttggg
     1861 tagctttttt ggtttctttc ctttgtattt cttttctctc tttttctctc tgcagagctt
     1921 ctttttccga gctcctttcc tgaagcagga gggataatga ggagtaatcc aatctcccga
     1981 gggaagcagg ggaggaaaga ggggctaagg aaaagaaagt atcgaggggg acacccatta
     2041 gttgatttaa attttcacgg gctaatttga gttctcgctc tttttcttgt agcaaaaatt
     2101 ttgagttttc cagattttcc tgggctttaa gcaaatcgat agaagaagca tttccttctt
     2161 cccatttctt tttttggtct tcaaagagaa tttgatacaa ttccagggtt ttttgagcta
     2221 tgttataatc tttttccccg tttagaatgt ttaaataata agtaacagct tgaactttag
     2281 ccgcttcttc ttcaaggaga agttggcttt cggcttcctg taaaagaatc tgagcggttt
     2341 tttcagtggg ggatttatca aaaagataaa cggcatcttt aatatttatc ccccaggtca
     2401 tatcgctcgt atcttccccg tttatgatag gccagttacc cgataaggta acctccgggg
     2461 ataaaccctg agaagcagaa gaagacaggt ttagcttagc agtttctact gcatctcgag
     2521 catttaacac ctctggagaa ttagctaaaa ccttttctac cacataagag aggtttatgg
     2581 tttctatttc cccggcctga gatagtcctc cagaaaaaca gctaaagagc cagagaaaaa
     2641 ccaccagatt tgctccccga ggaaaaagag aagttttaga ttttttcata attccatcag
     2701 ctccccttca gtatggtaaa tcattcgtag cgaagggcgg taatagggtc tagctggctt
     2761 gcccgacggg cggggtaaat tccaaagaaa agacctattc ccaccgaaat agaaaaggcg
     2821 ataattatcg cttccacaga tataatagcg gggtaagaag taaacttaga aatcacctgg
     2881 gaacccacaa ttcccataac tattcctatt agccctccta tggcgctcaa aagagcagat
     2941 tcaattacaa attgagctaa gatatcaaaa ggtcgggctc ctatggcttt ccgcaaccca
     3001 atctcccggg tgcgctcggt aaccgacacc agcatgatat tcataattcc gatacctccc
     3061 acgagaagag agatcccggt gatagcggtt aaaaataaag tcatggttcc agtaacctga
     3121 tttatagtat ctaaaatatc ttgctgattg atgatagaga atttttccgg gtctttcagc
     3181 cgattggtga aaaaagtttc taagaactga gctacgaaat ccaggtcttc ttctgtttct
     3241 gtctggatgg tcatactctg aatatattta ctgccagtta accttttttg catagtggtt
     3301 atgggaatga acacctgttc tcctaagtcc tggataccag tgcttttttc ctccatcact
     3361 ccgataactg tagctgttat tcttcccacc cggatttttt tccccagagc ttcttctgga
     3421 gaggaaaata ggtcttgagc tactgttctt cctaacactg ctactctttc tgcgttgtct
     3481 aattccttct ggttgaaaaa gcgtcccata tctactttaa aattgcgaac ttcgggatat
     3541 tgaggagtag tacctaccac tctccctctt atactttctc taccgtgagt tacagtgagt
     3601 gaggtggtat tttccgctat tacatttttg attcctggaa agccaaagtc ctgaagctgt
     3661 aaatagtgct cccattcaag catatttcct cctcgagaag cgactggcat tcctggtata
     3721 cctcggcttc tcccgggaat gacggtaatt tggttggaac ctaaacttcc aatctgacct
     3781 actatttctt cggttgttcc ttctcctaaa ctggtcatag ctactacagc ggctactccg
     3841 atgatgattc ctaacatagt aaggaaagaa cgaagcttat tggataggat actggcaatg
     3901 gcactttgca aagtctcaga cagattcaac tacttcttcc tccaccaatt cttttagaac
     3961 ctctaaggcc tccacaggtt gctctatagt ttcattatct actaattgcc cgtctctaaa
     4021 atggagaatc cggtgggtgt gagaggcgat atcccgttca tgagtaacta aaactatggt
     4081 tttgccttct tgattgagtt cctggagaat atgcattatc tcttcactcg agcgagtgtc
     4141 caggtttcct gtgggctcat cggcgagaat aatggagggg tcgttaacta aagctcgggc
     4201 aatggccact cgttgctgct gtcctccaga gagttgattg gggtagtgat gaatcctgtc
     4261 tcctaaacct actgcttcca aggattttat agcttgctct tttctttttt gagaaggaat
     4321 tcccgcataa attaaaggaa gttccacatt ttgtaaagca gttaaccgag gaagaagatt
     4381 gaagctctga aaaatgaaac ctattttttt gtttcgaatc tgggccagct gattttcatt
     4441 gagctgtgac acctcttgtc catcgagaaa ataacgaccc cgagtgggac ggtctagaca
     4501 gcctaaaata ttcatcaggg tagattttcc cgaaccggaa ggtcccatga tggcgacgaa
     4561 ttctccttct tctatttcaa aagacactcc ccggagagct tcaacttgca cttctcctaa
     4621 ctggtaaatt ttccagaggt cttctacttt tattactggt tgattcattg ttttcacctc
     4681 taattctacc ttcccccggg catgcccata ggaggaccaa aattagattg aaaagaagtg
     4741 gaagaaagga ttagaaaaac ttcttcgcct tcctgtagtc cttcttcaat ttccacctgg
     4801 gtatcgcttc tcaagcctgt ttttacttct ttttgtacag gttgtccatc aggacctttt
     4861 actaatactt gggcttttcc tcctctttct tccagagctt caagagggat gataatagca
     4921 ttatccttac ttttgatgat aatttctgcc tcagcggtca ttccagatag gagaatattg
     4981 tcggggttgg gtaactctat ggtggtttca aaaaccacta tattttctac tatttttcct
     5041 tctggagcaa tggtggttac ttttccctca aattgctggt tggggaaagc atcgagagta
     5101 accacggcct tctggccttt ttccacctct gggacatcga tttcatttat taatattttt
     5161 actttcatgg tggagag
//
LOCUS       DAOPSS010000114         5102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_13237_length_5102_cov_8.803249, whole genome shotgun sequence.
ACCESSION   DAOPSS010000114 DAOPSS010000000
VERSION     DAOPSS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13237_length_5102_cov_8.803249"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKN31_06745"
     CDS             complement(<1..839)
                     /locus_tag="PKN31_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOA99656.1"
                     /translation="MLACVFEKKEQWAIQEIEEPRLAPDEVLVRVKAAGICGTDVHIF
                     RAEYFSDFPIVAGHEFSGEVVKVGSEVTQFQPGDRVTADPNIFCDRCYFCKINKNNHC
                     LNLQAVGVTRNGAFAELVAVPEKCVFHLPANLSFSEGALVEPLSCVVYGVRRSNIQPG
                     EKVLIFGAGPIGLLLLSLFNVSGASQVVAVDVSEKKLNLAKKLGAQEVVLADGREGKR
                     LREIAPRGFEVVVDATGIPQVMEKEIEFVESDGTFLIFGVAPRDSAIAIDPYEVFQRD
                     LHIV"
     gene            complement(787..2259)
                     /locus_tag="PKN31_06750"
     CDS             complement(787..2259)
                     /locus_tag="PKN31_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873143.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOA99657.1"
                     /translation="MQVKIIVLLIYVAGMILIGYFSMKKTKTVGDFFLANRSLGPWVS
                     AFAYGSSYFSAVLFIGYAGNIGWGFGLSSLWIVVGNTLIGSLLAWLVLARRTRLVTEE
                     MQVMTMPEFLQVRYGSNFLKAFSALLIFVFLVPYSASVYMGLSYLFDVVMGIPYFYAL
                     LLIAGLTGVYLVMGGYLAVSITDFFQGSIMLGGAIFLIVYLVGRPEVGGWSQIVARLR
                     EVDPQLVGAVGPPGFWPLFGLVGLTSLGPWGLPQMVQKFYALKNEKIVYTATILATIF
                     ALICTFSAYLSGSLTHLFFDQLPQINGEPNPELLMPHLIAEVMPPAFSLIFLLLVFSA
                     SMSTLSSLVLVSSSAITIDLLPARFRDKSLPIMRVLCGVFIFFSLLIAFYRVTFIVNL
                     MSISWGAVAGSFLAPYLYGLYWKGANRWGAISSMIAGLVVSLGFSWYYGFNSPLAPLW
                     GSLAMLVPLVVLPVVSILTGGEEDVSLRIREKRAVGNTGN"
     assembly_gap    2403..2502
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2503..2613
                     /locus_tag="PKN31_06755"
     CDS             <2503..2613
                     /locus_tag="PKN31_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOA99658.1"
                     /translation="INPLLVLFGQNICLPRRPRCSHCPVEEFCEKRGVEN"
     gene            2610..3377
                     /locus_tag="PKN31_06760"
     CDS             2610..3377
                     /locus_tag="PKN31_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99659.1"
                     /translation="MNYLWYIATTILIAFLLFVVLFFRKSKFTKKKLLLYYLLSIALP
                     LVIPYRWVEKILFDYLAGLPSALFISLVLILIEWALLFFLIMKANQEMLDAPEEKLFL
                     FLLLGLGFGLGKALRDTLLVYFHSLSAFYGYSQFANFPSFFYILQKIMEAQIEVISAG
                     LLGLAFLSPAGYTFHHLVSYSLLSFIPKGLITLAQVLIQFVPSLSFLASVYLISLLPL
                     TLIIGFGLLWYLYRRRLYQIIQEDETSPHEENFKEGA"
     gene            3379..4065
                     /locus_tag="PKN31_06765"
     CDS             3379..4065
                     /locus_tag="PKN31_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99660.1"
                     /translation="MRKFFSGNKSLSVIVGVVIFLINFMVISSPQAKETFSYKIMVQE
                     KEIGKMIYEVEEENNFLQIKSFIKLAEEEMERDAQLTILRDTYEPLRSQKWVKLPGGE
                     IVIETTYEERKAHIKISSPEGTKEATISLPSPVFDSEEIPYLIGKLSCEQEKVISVLV
                     PISGMVWRGKIQKIAENEEEEIFRFTLGGEITYLRYQKEKPRLVELQAPQRGYNLVLE
                     DKPAPREENN"
     gene            complement(4716..5003)
                     /locus_tag="PKN31_06770"
     CDS             complement(4716..5003)
                     /locus_tag="PKN31_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99661.1"
                     /translation="MPNTLAIYLDETHLRANNLFYDVELSREIEKTLKIPVDIVILNH
                     APDRLVYRALQGLLIKDEDEEFRSDFLLLRWRDSGFSRSHKKIPAGAQKCQ"
BASE COUNT         1606 a         1167 c          872 g         1357 t
ORIGIN      
        1 acaatatgta aatcccgctg gaaaacctca taagggtcga tggctatcgc ggagtctcga
       61 ggagccactc caaaaatcaa aaaagtacca tctgattcta caaattctat ctccttttcc
      121 ataacttgag gaatccctgt ggcatctacc accacctcaa atccccgagg ggcaatttcc
      181 cttaatcttt taccttctct accgtcagct aaaactactt cctgagctcc caactttttg
      241 gccaaattca gtttcttctc acttacgtcc actgccacca cctgagaagc tccacttaca
      301 ttaaacagag acaaaagtaa aagcccgata ggacctgctc caaaaatgag cactttttct
      361 cccggctgga tattgcttct cctcacccca tacaccacgc aagaaagagg ttcaaccagc
      421 gctccttctg aaaaacttag attagcagga agatgaaaaa cacatttctc cggaacagcc
      481 accaattcag caaaagcacc gtttctcgtt actcccaccg cttgcagatt cagacaatga
      541 ttgtttttgt ttattttgca aaaataacaa cggtcacaaa aaatattggg gtcagcggta
      601 acccggtctc ctggctgaaa ctgagtaact tcgctcccca ctttaaccac ttctccagaa
      661 aattcatgcc cggccactat ggggaaatca gaaaaatatt cggcgcgaaa aatatgaaca
      721 tcagtcccgc agattcccgc tgcctttacc cgcaccagaa cctcatctgg cgcaagacga
      781 ggttcttcaa tttcctgtat tgcccactgc tcttttttct cgaatacgca agctaacatc
      841 ctcttcacct cctgttaaaa tagaaactac aggtaacacc accaaaggaa cgagcatagc
      901 taaagacccc caaagaggcg ccaggggaga attgaatcca taataccagg aaaaacccag
      961 agaaaccaca agacccgcaa tcatactaga aatagccccc caccgattag ctcccttcca
     1021 gtatagacca taaagataag gagctaaaaa actccccgcc actgctcccc aggaaataga
     1081 cattaagttc acaataaaag taacccggta aaaagcaatt aaaagagaga aaaaaataaa
     1141 taccccgcac aaaaccctca taataggtaa agacttatcc cgaaaacgag ccggtaacaa
     1201 atcaatagtg atagcagaag aagaaaccag aaccagcgag gaaagagtag acatagaagc
     1261 agaaaatacc agaagaagaa aaattaagga aaaagcagga ggcataactt cagcaattaa
     1321 atgaggcatt aaaagctccg ggttgggctc tccgttaatc tggggaagct ggtcgaaaaa
     1381 tagatgagtc agggaaccag ataaataggc actaaaagtg caaatcaaag caaaaatagt
     1441 ggccagtata gtggcagtgt aaactatttt ttcatttttc aaagcataaa atttctgcac
     1501 catctggggt aaaccccagg ggcctaaact agtcaaaccc acaagaccaa aaagaggcca
     1561 gaaacccgga gggcctactg ctcctaccag ttgcggatct acctcccgga gacgagctac
     1621 aatctggctc catcccccca cctccggacg acccacgaga taaactatca aaaatattgc
     1681 ccctcccagc attatactac cctggaaaaa atcggtaatg cttaccgcaa gatatcctcc
     1741 catcaccaga tataccccgg ttaaccctgc tatcaacaaa agagcataaa aataaggaat
     1801 tcccatcact acatcaaaaa gatagcttaa acccatataa accgaagcag aataaggaac
     1861 taaaaacaca aaaattaaaa gagcagaaaa agccttcaaa aaattactac cgtagcgaac
     1921 ctgcaaaaac tcaggcatgg tcataacctg catttcttca gttactaatc gggtgcgtcg
     1981 agccagaacc agccaggcca gaagagaacc tatcaaagtg tttcccacca caatccataa
     2041 actggaaaga ccaaatcccc aaccaatatt tcccgcatat ccaataaaaa gaacagcact
     2101 gaagtagctt gagccataag caaaggcaga aacccagggg cctaaactgc ggttagccag
     2161 aaagaaatcc cccaccgttt tagttttctt catgctgaaa taacctatga gtatcatacc
     2221 tgccacataa atcagtaaaa caattatttt gacttgcatt ttttgccccc ctgcgtttat
     2281 tattattcta tgaatgatga aaatttaagt aaagcttttg ctattttaca ggaaataaag
     2341 ggacaatggc aagacaacac catcaatagt ttaggtaatg accctttttt agtcctggtt
     2401 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcaatcc tcttttagtc
     2521 ttatttgggc agaatatctg tcttccccgt cgtccccgtt gttctcattg ccctgtagaa
     2581 gaattctgtg aaaaaagagg ggtggaaaat tgaattattt atggtatatc gcaactacaa
     2641 tattaatagc ctttttgctt tttgtagttc tattctttag aaaaagtaag tttaccaaga
     2701 aaaagttact tttgtattac ctcttatcca tagctttacc tcttgtgatt ccttatcggt
     2761 gggtagaaaa aatactcttt gattacttag ccgggctacc ttctgctctt ttcatatcct
     2821 tagttttaat actcatcgaa tgggctttgc ttttttttct gataatgaaa gccaatcagg
     2881 aaatgcttga cgctccagaa gaaaagcttt ttctcttcct tctcttaggg ctgggctttg
     2941 gcctggggaa agcgttacga gatacacttc tcgtttattt ccattctctc tctgcattct
     3001 atggatatag tcagtttgcc aattttccct ccttttttta tatcttgcag aaaattatgg
     3061 aagctcaaat tgaagtaata tccgcaggcc ttttaggtct ggctttcctc tcccctgcag
     3121 gatatacttt tcaccacctg gtttcttatt ctctcctctc tttcataccc aaagggctga
     3181 taactctggc acaggtttta atccagttcg ttccttccct ttccttctta gcttcagttt
     3241 atctcatcag tcttcttcct cttactttaa tcatcggatt tggtcttttg tggtatcttt
     3301 atcgtcgtcg actttatcaa atcatccagg aagacgaaac atctcctcac gaagaaaatt
     3361 tcaaggaggg agcctgaaat gaggaaattt ttttccggga ataaatcact ctctgttatc
     3421 gtaggagtgg taatcttttt aataaatttt atggtaattt cttccccgca agcgaaagag
     3481 acctttagct ataaaataat ggtgcaagaa aaagaaatag gaaaaatgat ttatgaagtg
     3541 gaagaagaaa acaatttttt gcaaatcaag tcttttatta agttagccga agaagaaatg
     3601 gagcgagatg ctcaactcac tatcttgcgg gacacttatg aacctctccg ctctcaaaag
     3661 tgggtaaagc ttcccggggg agaaatagtg atagaaacta catacgagga gaggaaagct
     3721 cacattaaaa tctcctctcc tgaagggact aaagaagcta ccatctctct tccctcccct
     3781 gtttttgata gtgaagaaat accttattta ataggaaaac tgtcttgcga gcaagaaaaa
     3841 gttatatcag tattagttcc catctcggga atggtatgga ggggaaaaat tcaaaaaatc
     3901 gccgaaaacg aagaagagga aattttccga ttcactttag gaggagaaat tacctacctt
     3961 cgctatcaaa aagaaaaacc ccgccttgtg gagctccagg ctccccagcg gggctacaac
     4021 ttagtgctgg aagataagcc tgctccaaga gaggagaaca actaactcat ccggaattgt
     4081 ttaataactt tcaatggtca gggtttcccc attgtagtgt aaacggtgat cgtctttcta
     4141 tcacaagtgg gaataaaccg aacccaccaa acctcacttc cccggatttc cacacgatgc
     4201 tcttcgtcca aagtcataag aattcaactt gcacaataag accttccacc catcaatgta
     4261 ttcacctatg ccgtcaccat aaatgtaaac ccaaccagca atcactgacc cttcacgagc
     4321 acatattagt caataaatgc atcaatatct tgaagttggg attgtagtat atgatagagt
     4381 atttcattgt caactttcca gtaaagatgg actgactaac aaatttctga atttagccat
     4441 attggctaag ttctgcacca ggttttccag tgtataatta ctctttcttt cagcaggata
     4501 aaaccatcgg catgccttct tggcacgccc cattgttcac gagataaaat atgattacaa
     4561 acatcgatac gtccttccat aaccacaatg aagcaatact taacacttcc cagatattag
     4621 ctcatcaata attttctctc cctcttttta gaaatccttc aaaatattta gagctttctc
     4681 aatctctcct aaaaggtaaa caattctttc tctatctact ggcacttttg agctcctgcc
     4741 ggtatttttt tatgacttct tgaaaatcca gaatctctcc atcgaagaag aaggaaatcc
     4801 gaacggaatt cttcgtcctc atcttttata agaagacctt gcaaggctct atataccagg
     4861 cggtcgggag cgtgattcag tataactata tctaccggta ttttaagggt tttttctatt
     4921 tctcttgata attcaacgtc ataaaataag ttatttgccc ggagatgagt ttcatcaaga
     4981 tatattgcta atgtatttgg caatatgaat ttgcaacatt ccggaagtta atttttgcag
     5041 caccttcaat tccagaatta acagttcata gtagaatgag gcaaaggata acttggttcg
     5101 ga
//
LOCUS       DAOPSS010000115         4976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_13676_length_4976_cov_8.225157, whole genome shotgun sequence.
ACCESSION   DAOPSS010000115 DAOPSS010000000
VERSION     DAOPSS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13676_length_4976_cov_8.225157"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /gene="purL"
                     /locus_tag="PKN31_06775"
     CDS             complement(<1..2104)
                     /gene="purL"
                     /locus_tag="PKN31_06775"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOA99662.1"
                     /translation="MEVANGLNVARELGLKEEEYERILAILGRVPTPTEIAMFSVEWS
                     EHCGYLHSRRWLELLPREGEFSPLRGEDAGGIVYDDLAVVFKMESHNHPSQVEPRQGA
                     ATGIGGVIRDILGSGARPIACLDSLHFGPREDERSLYVFRGVVEGVAFYGNCVGVPTV
                     AGELYFYPAFRGNCLINVMCLGIAPREKLTQAKAQGEGNYIVYVGNKTGRDGIGGCSI
                     LASQEFGAEERRPSVQIGDPLAEKCLIEATLEALDTGHIVGIKDMGAAGLTCSTSEMA
                     ASGGSGVEIDLDLVPLREEGMEPWEIMMSESQERMLLCVRRGYEEEIIQIFRKWGLEG
                     EVIGEVKDTGRVTIKWRGEVVADIPALELTRPPVYTPPRERPLYLDEVNNFSLEDVSP
                     PLNYEEVLLRMVSSENLSSRRPIFEQYDHMAGINTVLLPGSGTVVLRVKGKKWGIAVT
                     SDCNPLYCYLSPREGAKMAVAEAARNLSACGARPAGVTDCLNFGSPEKPDRYWQFVEA
                     VEGLSEACRFFGIPVVSGNVSFYNENPEGAIFPTPTIGMVGIIEEVEKVVSAGFGEDD
                     LILVLLGETKEELGGSEYLRIIHNREEGPPPRVDLEGEKNLQELLITLAEEEIVSSAI
                     DLSEGGLALALVESSVTEENPRGVIVDLECLGDMREDVLLFGESGGRALVSVKGELLN
                     SLQRLAQEQNVPCQVIGKT"
     gene            complement(2095..2793)
                     /gene="purQ"
                     /locus_tag="PKN31_06780"
     CDS             complement(2095..2793)
                     /gene="purQ"
                     /locus_tag="PKN31_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOA99663.1"
                     /translation="MKFGIVVFPGSDCGYDCHHVLSQVLEAETVFIWHGDKNLKNCDC
                     VVLPGGFSYGDYLRAGALARFSPVMEAVKEFAENGGLVIGICNGFQVLTEAGILPGAF
                     LPNRGGRFICRHVYLRVENNQTPFTLLFAPGEVIKIPIAHQQGNFFIDSPHQEEVKKH
                     IVFRYCGPQGEIDSRYNPNGSWENVAGIVSREGNILGIMPHPERAAEEILGSEDGRRV
                     FLSVIKWWEDKKWK"
     gene            complement(2802..3059)
                     /gene="purS"
                     /locus_tag="PKN31_06785"
     CDS             complement(2802..3059)
                     /gene="purS"
                     /locus_tag="PKN31_06785"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOA99664.1"
                     /translation="MKYRGKVIVTLKPGVFDPQGVTIRNALHTLSFREVEEVKAGKYL
                     EIILESENCSQAEKRIQEMCDQLLSNPVIESYSYQLEEIKN"
     gene            complement(3087..4289)
                     /locus_tag="PKN31_06790"
     CDS             complement(3087..4289)
                     /locus_tag="PKN31_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA99665.1"
                     /translation="MSEKVGFVTMGEAKGIVENLPELGVGMLGYAFMGKAHTNAYKKY
                     PYIFWPPVAIPRLVAICGRNEKAVREAMVRYGYERYYTDWEEIIEDPAVQILDNTSPN
                     YLHAEPSILALEKGKHVLCEKPLAGNAQEAKKMWEAAQKSKAKAMVCFNYRFVPALRL
                     AKEFISSGKLGEIYHFRAQYLQEWIMDPDFPAVWRLDKNAAGSGALGDLGAHIIDLAR
                     FLVGEITTVSALTRTFIKERKLAEDPRKKVPVTVDDAFVALVEFEKGAIGTLEASRFC
                     AGRKNYQVVEINGSRGSIRFNLERLNELEVYLVGEEPKIFQGFHDVLVTESFHPFYEY
                     WWPQGHIIGWEHTFVHAVAHFLDAIVNNKDIAPYGATFEDGYRCAVICDAILQAAESG
                     KREIVPQD"
     gene            complement(4282..4662)
                     /locus_tag="PKN31_06795"
     CDS             complement(4282..4662)
                     /locus_tag="PKN31_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99666.1"
                     /translation="MDTISKGRMFRSHIESRYPFGEFALIRSRGNLGEVGFVLADEDA
                     ENWPSLYREAWEIASYFRLRIKELGLDNIAMAGVGRDLDFITVSIVVDLSALEKEEIE
                     KIADQVMGILREANPYNKGGGINE"
BASE COUNT         1439 a         1304 c          825 g         1408 t
ORIGIN      
        1 cggttttccc tataacctgg caaggaacat tttgttcttg agccaggcgc tgcagggaat
       61 ttaataattc tcccttcaca ctcacgagcg ctcttcctcc gctttctcca aaaagcaaaa
      121 catcttctct catatccccc aggcattcca gatccacaat tactccccga ggattttctt
      181 ccgttacaga agattctaca agagccagag ccagccctcc ttcactcaaa tcaatagctg
      241 aggagacaat ttcttcttct gccaaagtta taagaagttc ttgcaggttt ttttcgcctt
      301 ctaaatctac ccggggaggc ggcccttctt ctcgattatg aataatcctc agatattcac
      361 tccctcccag ttcttctttt gtttcaccta aaagaactaa aattaaatca tcttccccga
      421 acccagcact taccaccttt tccacttctt ctattatccc caccattcct atagtggggg
      481 tagggaaaat cgctccttca ggattttcgt tataaaagga tacatttccg ctcactaccg
      541 gaataccaaa gaaacgacag gcttcgctca agccctcaac agcttctaca aactgccaat
      601 aacggtcagg tttttcagga ctaccaaaat tcaggcaatc agtaactcca gcaggccttg
      661 ctccacaagc actcaaattc cgggctgctt cagccactgc cattttcgct ccctctcgag
      721 gagaaagata acaataaagg ggattgcaat cactggtaac ggctattccc cacttcttgc
      781 ctttcactct caacactaca gttcctgatc caggaagaag aacggtatta attcctgcca
      841 tatggtcgta ttgttcaaaa atgggacggc gagaagaaag attttctgaa gaaaccatgc
      901 gcaataaaac ttcttcgtag ttgagaggag gtgaaacatc ctccagagaa aaattgttaa
      961 cctcgtccag ataaagaggc ctctcccgag gaggagtata aacggggggt ctggttaact
     1021 ccagagctgg gatatcagca actacttctc ctcgccactt tatcgttacc cttcctgtat
     1081 ccttaacttc ccctattact tctccctcta acccccattt tcggaaaatc tgaataattt
     1141 cctcttcata tcctctcctg acacacagaa gcatccgctc ttgagattca gacatcatta
     1201 tctcccaggg ttccatccct tcttctcgaa gaggaactaa atctaaatca atttctaccc
     1261 cacttcctcc actagcagcc atttcactcg tggaacaggt aagtcctgct gctcccatgt
     1321 ctttaattcc tactatatgt ccggtatcta aagcttctaa ggtagcctcg atcagacatt
     1381 tttccgccag gggatcacca atctgcacac taggtcttct ttcttctgct ccaaattctt
     1441 gagaagctaa gatgctacat cctcctattc catctcttcc ggttttatta cccacataaa
     1501 caatataatt tccctctcct tgcgctttag cctgagttaa cttctcccgg ggagcaatac
     1561 ccagacacat cacgtttatc aggcaatttc cccgaaaagc agggtaaaaa tacagctctc
     1621 cagcaactgt gggtactccc acacaattac cgtaaaaagc tactccctcc actactccac
     1681 gaaacacata caaagaacgc tcatcttccc gaggaccaaa atgcaaagaa tcgagacaag
     1741 caataggccg tgctccgctt cccaatatat ctcgaataac acctccaatt cctgtagctg
     1801 ccccctgcct gggctctacc tgagaagggt gattgtgact ttccatttta aaaaccaccg
     1861 ctaaatcatc ataaactatg ccaccagcgt cctctccccg aagaggagaa aattctccct
     1921 ctcggggtag caactccaac catcttcgag aatgaagata accacagtgt tccgaccact
     1981 ctacagagaa catagctatt tcagtaggag taggaacccg ccctaatata gcaagaattc
     2041 tttcatattc ctcttccttt aatcctaatt ccctggcaac attcaagcca tttgctactt
     2101 ccacttttta tcctcccacc attttataac tgaaagaaac actctccggc catcttcgga
     2161 gcccaaaatc tcctctgctg ctcgctcagg atggggcatt atacctaata tattaccttc
     2221 tctggagact atccccgcca cattttccca cgaaccattg ggattataac gagaatctat
     2281 ttctccttga ggaccacaat aacgaaaaac tatgtgcttt ttaacttctt cttgatgagg
     2341 agaatcaata aaaaagttgc cttgctgatg agcaatgggt atttttatta cctctcccgg
     2401 ggcaaataaa agagtaaaag gtgtttgatt attttctact cgtaaataga catggcggca
     2461 aataaaacgt ccacctctgt tgggaagaaa agctccaggt aaaatcccgg cttcagtgag
     2521 aacttgaaaa ccgttacata ttcctatgac taatcctcca ttttcagcaa actcttttac
     2581 cgcttccatc actggcgaaa aacgggcaag agcccctgct cgaagataat cgccataact
     2641 aaaacccccc ggcaatacta cacagtcaca atttttcaga tttttatctc catgccagat
     2701 aaatactgtt tctgcttcca agacttgaga taaaacatga tgacaatcgt aaccacaatc
     2761 agaccctgga aaaaccacta ttccaaattt cattttcatc ctcaattttt tatttcttct
     2821 aactggtaag agtagctttc gattaccggg ttagacaaaa gctggtcgca catttcctga
     2881 atgcgctttt ctgcctgaga gcagttttcc gattctaaaa ttatttccaa gtatttacca
     2941 gccttaactt cttctacttc tcgaaaagac aaggtatgca aagcattacg aatggtaact
     3001 ccctgtgggt caaaaactcc cggtttcaaa gtcactatta ctttacctcg atatttcata
     3061 gctcccccct gcaaataaaa tttattttaa tcctgtggta ctatttctct tttacctgat
     3121 tctgctgcct ggagaatggc atcacagatt actgcacatc ggtaaccatc ctcgaaagta
     3181 gctccatacg gagcaatatc tttgttatta actatggcat ccagaaaatg agccactgca
     3241 tgaacaaaag tatgttccca gccgatgata tgtccctgag gccaccagta ctcatagaaa
     3301 gggtggaagc tttcggtaac taaaacatcg tgaaacccct gaaatatctt aggctcttct
     3361 cccaccagat aaacttccag ttcatttaat ctttctaaat tgaagcgaat actacctcga
     3421 gacccattaa tctctacaac ctgataattt tttcttccag cacaaaaacg ggaagcttcc
     3481 agcgttccta tggctccctt ctcaaactcc acaagagcta caaaagcatc atctacagtt
     3541 acaggaactt tcttcctggg gtcttctgct aattttcttt ctttaatgaa agtgcgggtt
     3601 aaagcactaa ctgtagtaat ttctcccact aaaaaacggg ctaaatctat gatatgagct
     3661 cccagatctc ccagagctcc tgaacctgca gcatttttat ccaatctcca gaccgcggga
     3721 aaatcagggt ccataatcca ctcttgaaga tactgcgccc ggaagtgata tatctccccc
     3781 agcttccctg aagagataaa ttctttggct aatcgaagag caggaacaaa acgatagtta
     3841 aaacatacca tggctttagc tttagatttt tgagctgctt cccacatctt tttagcttct
     3901 tgcgcattac cagctaaagg cttttcacaa aggacgtgtt tgcctttctc taaagctaaa
     3961 atagagggct cagcatgaag ataattaggg ctagtgttat ccagaatttg cactgccggg
     4021 tcttcaatta tttcttccca atcggtgtag tatctttcat aaccataccg taccatagct
     4081 tcccttactg ctttttcatt ccttccacaa atggctacta acctgggaat tgccaccggt
     4141 ggccagaaaa tataagggta cttcttataa gcgttggtgt gagcttttcc cataaaggca
     4201 taacccagca tccccacccc taactcggga aggttttcta ctattccttt tgcctcaccc
     4261 atggtaacaa atcctacttt ttcactcatt gattcctccc cctttattat aaggattggc
     4321 ttccctcaaa atacccataa cttgatcggc aattttttcg atctcttcct tctccagggc
     4381 ggacaggtct actacaatgg aaacagtgat aaaatctaaa tctctcccca ctccggccat
     4441 ggctatgtta tccagcccca actctttaat tctcaagcgg aaataactgg ctatttccca
     4501 ggcttcccgg tagagagaag gccaattttc tgcatcttca tcagccagaa caaagcccac
     4561 ctctcccagg tttcctcggg aacggatcaa agcaaattcc ccaaaagggt atcgagattc
     4621 aatatgagag cgaaacattc ttcctttaga aatagtatcc attggcctct taaagtatta
     4681 taacttaact tctctgacaa ataaacaggc agcttacggc gtgagataaa agaatttaaa
     4741 aaatacagca gaacaaaaaa ccactgagga agaaagagct tctcggttcc taacaacata
     4801 ataaaaaagt gggataagct caaaagatta ttgagagagg tgtttttctc ccctgggtct
     4861 ggaatataat tttttaaaag gtgttttcac taaaaagtag gactttaaat aaagcttgct
     4921 ttgctacatt tttacaccat taaagaggag ggaaaaacat ggccgagttc aaaaag
//
LOCUS       DAOPSS010000116         4968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_13706_length_4968_cov_7.309587, whole genome shotgun sequence.
ACCESSION   DAOPSS010000116 DAOPSS010000000
VERSION     DAOPSS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13706_length_4968_cov_7.309587"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(537..1838)
                     /locus_tag="PKN31_06800"
     CDS             complement(537..1838)
                     /locus_tag="PKN31_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HOA99667.1"
                     /translation="MLRRDKVDQSYIRRKAEEEGVPVVDFLLRKMKELEKVEGRPSTL
                     FAACPNSETVIKAALRSAKRANAPIKFAATLNQVDEDGGYTGLTQSEFVEMVKIEAEA
                     ISYEGLIIVALDHGGPWLKDQHSIENWSLEETMTAVKKSLEAAIDAGYDFLHIDPTVD
                     KTLPPGEIIPIERVVERTIELIKHAENYRREKGIAPLGYEVGTEEVHGGLANLEVFDR
                     FLSLLREGLEKEGLSEVWPILIVGKVGTDLHTTIFDPEVAKTLAEKAAKYGSFIKGHY
                     TDYVENPEEYPLSGMGAANVGPEFTEEEYNALTQLAQLEDKLKKEKLIARSSLILQVL
                     EKEVYESNRWQKWLKGEEKGKDLYDLSPSRQKWLIKTGCRYIWAQPRVVASRTLLYRN
                     LEQNGYRGEEMVLLQVERAMDKYFRTFNLIDLHDRLKKVVE"
     gene            complement(1855..2790)
                     /locus_tag="PKN31_06805"
     CDS             complement(1855..2790)
                     /locus_tag="PKN31_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOA99668.1"
                     /translation="MRVGCLGELLVEIMRDRVDVPFDVPDIFVGPFPSGAPAIFADCL
                     ARLGEEVFFVGAVGEDDFGKMILRRLKEDGVDIGGVKILPDFTTGVAFVTYFRDGSRK
                     FIYHISQAAAGQIFPSDIDEEQFSRLDYLHVMGSSMLINDNCCDAYLRAIEIVKKKGG
                     KISFDPNFRPELLGAGKARALFQPIVQESFVLFPTQEELEVLSGEKDVDKGTAVLLSQ
                     GPEIIALKQGKKGSTIFTSKEKLFIPAFPVEEVDPTGAGDCYCAGFLAGLSRGLPLIE
                     VGRLANAVGALAVTKKGPMEGAPTQKELEEFLKKF"
     assembly_gap    2893..2992
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3192..3536)
                     /locus_tag="PKN31_06810"
     CDS             complement(3192..3536)
                     /locus_tag="PKN31_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOA99669.1"
                     /translation="MDEAELFHYWNKRLKEEGLGVYKGGREILLPDLIEKYSAPFKEK
                     RKNISLPLEVLSPRQREAIYLLFYDGLSEAEAAETLGISRSSLRTHRDRALEKLKTEL
                     EKNWWRKDNEVK"
     gene            3628..4029
                     /locus_tag="PKN31_06815"
     CDS             3628..4029
                     /locus_tag="PKN31_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOA99670.1"
                     /translation="MFYNLLVMGDWQDKGQRLRELRNKKGWSVRGLAREMGVSKSTVS
                     DVELGRRKPTANYLIKAAKTLGIAERELLEWYGYLEPLPPDLDQKLERVAREFDTLFL
                     DHLGELNEESKKSLVDFLEYLIEKQKREDRD"
     gene            4041..4451
                     /locus_tag="PKN31_06820"
     CDS             4041..4451
                     /locus_tag="PKN31_06820"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOA99671.1"
                     /translation="MISLSPESIEKIESFSSSLPRINSEIEALKLAEKEGIIVEFYDL
                     RGFKGVLAQRRGQTFLGVNQRLREAEEVKTILHELAHYFFHWNNRFRPIFLCQEYTIN
                     REEKEADFFAWCLVEECWRETLSWKFWARDRDFS"
     gene            complement(4687..>4968)
                     /locus_tag="PKN31_06825"
     CDS             complement(4687..>4968)
                     /locus_tag="PKN31_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99672.1"
                     /translation="EEVERNRVKLRIYWIGSSSTKKMLSGLWRAFRFLLMITWQRGAY
                     VWPKRREDIRLFSFLRKELITFVVSVVYLYSEKTRQECIGVFRPSFSVI"
BASE COUNT         1263 a         1199 c         1008 g         1398 t
ORIGIN      
        1 ggtttgtagt aatttttatg ctgtactttt taacttccgc tgtgttgcat ttctactcta
       61 ccaaacacat cgggtcaaca cataaaaagg caaaataaac cacgttggaa gaaaaacagc
      121 cagtgtcgcg gggatctctc ttctggattc ctttcttttt tctcccttcc ggggagggct
      181 ttataatata ttctttctct caccctcacc ccggcaactt gcgagccgta tttagcctgc
      241 ccctgaatgc ttctgcgagg ggtccagaag tatttgaaaa ccatggattc ccgactaaag
      301 attcaggaaa caacggaagg aggaatcatc acccctgagt gggttctgtc acttccctta
      361 tctgtcattc ctgaatggtt ctatcaggaa tctaaattat ttaaagacaa aaacatttgg
      421 atttccgaca aaagatttcg gaaatgaggg ggaagaaacc acaggaaacg ttccccaccc
      481 atagccgccg cgaacaaagc taaccgaggg aggggcttcc ctcggttagc ttcttattac
      541 tcaactactt ttttcaggcg gtcgtggagg tcgatgaggt taaaagtccg gaaatattta
      601 tccatagctc tctccacctg taaaaggacc atttcctctc cccggtaacc gttttgttct
      661 aagttgcgat aaagaagagt gcgactggcc actacccgag gttgagccca gatatagcgg
      721 caaccagttt ttatcagcca cttctggcga gagggagaga gatcgtataa gtctttgcct
      781 ttttcttctc cttttaacca cttttgccag cgattactct cgtatacctc tttttccaga
      841 acctgaagaa tcaaagagga acgagcaatg agcttctctt ttttcaattt atcttccagc
      901 tgagccagct gagtcaaggc attgtattct tcttcggtaa attccggacc cacgtttgct
      961 gctcccatcc ccgagagagg gtattcttca gggttttcca cataatcggt ataatgtcct
     1021 ttgataaaag agccatattt tgccgctttt tcagcgagtg tctttgccac ctcagggtca
     1081 aaaatggtgg tgtgaaggtc tgtccccact tttcctacga tgagtatggg ccacacctcc
     1141 gataacccct ctttttctaa gccttccctg aggagggaga gaaagcggtc gaacacttca
     1201 agattggcca gccctccgtg aacctcttct gtccccacct cataacccag gggggcaatc
     1261 cctttttctc ggcggtagtt ttcagcatgt tttatcagct ctatggttct ctctactacc
     1321 cgctctatgg ggataatctc tcccggggga agagttttat ctactgtggg atcaatgtgg
     1381 agaaaatcat atcctgcatc tatcgctgct tccagcgatt ttttaaccgc tgtcatggtt
     1441 tcttcgagac tccagttttc tatactgtgt tggtctttaa gccagggtcc tccgtggtcg
     1501 agggcaacga tgatcagccc ctcataacta atggcctcgg cttctatttt taccatttcc
     1561 acaaactcag attgtgtaag gccggtatat cctccatctt catccacctg gtttaaagtg
     1621 gcagcaaact taatgggggc attggctctc tttgccgaac gcagtgcagc cttaataacc
     1681 gtctcagaat taggacaagc agcaaagagg gtggaaggcc tcccctccac tttttctaat
     1741 tctttcattt tgcgcagcaa aaaatcaacc accgggactc cttcttcttc tgccttgcgg
     1801 cggatatagc tctggtctac tttatctctt ctcaacaaaa gacttctctc cttttcaaaa
     1861 ttttttcaaa aactcttcta actctttttg agtgggcgct ccctccatgg ggcctttttt
     1921 ggttacagct aaagctccta ccgcattagc taacctgccc acctctataa ggggcagccc
     1981 ccgggaaagg ccggctaaaa acccggcaca gtagcaatca ccagctccag tggggtctac
     2041 ttcctccacc gggaaggcag ggataaataa tttttcttta gaggtaaaaa tggtggaacc
     2101 ctttttcccc tgtttcaagg caattatttc tggcccctga gatagcaaca ccgctgtccc
     2161 cttgtccaca tctttttctc cacttagcac ttccagctct tcctgcgtgg gaaaaagaac
     2221 aaaactctcc tgcacaatgg gctggaacag cgctcgagcc ttacctgctc ccagaagctc
     2281 ggggcggaaa ttgggatcaa aactgatttt tcctcccttt ttcttcacta tttctattgc
     2341 tcgaaggtaa gcatcgcagc aattatcatt gatgagcatg ctggagccca tgacatgcag
     2401 gtaatccaga cgagaaaact gttcttcatc aatatcagag gggaatatct ggccagcagc
     2461 agcttgagat atgtgataaa tgaattttcg gcttccgtct cggaaatagg tgacgaaagc
     2521 tacaccggtc gtaaaatcgg gaagaatctt aacccctcca atgtccactc catcttcttt
     2581 caatctccgg agaatcattt tcccgaagtc gtcttctccc accgctccta caaagaaaac
     2641 ttcttctcct aaacgagcca ggcaatcggc aaaaatagct ggagcaccgc tcgggaaagg
     2701 ccctacaaaa atatcgggca cgtcgaaggg tacatctact ctgtctctca ttatttctac
     2761 cagaagctct cctaaacaac ctactcgcat aagcctctct cctttcagga ttttataatt
     2821 actttaattc tctttttttt ccattgcttc catagcttcc cctattttct ccagaggaaa
     2881 agggtgagcg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgtcccg
     3001 gaataacagg aaacctgaca ttggtttttc cctgctataa gcttcgtagt cgatgagaca
     3061 tgtcccgcag gtctctaact catacctatg gattagaggg taagaagggg agaccccttt
     3121 taccacaagc tttttgaccc cttctaaccc agcagtcaac aaagaaacca actccccttt
     3181 taatcttgct tctattttac ctcattatcc tttctccacc aattcttttc cagctctgtt
     3241 ttaagtttct ccagggctct atcgcggtgg gtgcggagag aggagcgaga gatgcccagt
     3301 gtttctgctg cttctgcctc cgatagccca tcgtagaaga gaagataaat cgcctctcgc
     3361 tggcgagggc tgagaacctc aagaggtagg gagatgtttt tcctcttttc tttgaaaggc
     3421 gcagagtatt tttctatgag gtcgggtaaa agtatctctc ttcctccttt atatactccc
     3481 agtccttctt ccttcaatcg tttgttccag tagtggaaaa gttctgcctc gtccataagc
     3541 acctccttat ttgtccttat tataggacta taagtcctgt gggtcaacaa aagaattttt
     3601 gaattccctc tttctttttg gagataagtg ttctataatt tacttgttat gggcgactgg
     3661 caggacaaag gacagagatt aagagagctg agaaataaaa agggttggtc ggtcagggga
     3721 ttagctcgag aaatgggagt ttccaaatct accgtttctg acgtggagtt aggtagaaga
     3781 aaacccaccg ccaactatct gataaaggca gccaaaactc tgggcatagc agagagagag
     3841 ctgctggagt ggtatggcta tctggagcct ctgcctcccg atttagacca gaaactagaa
     3901 cgggtggctc gagagtttga taccctcttt ttagaccacc taggggagct aaacgaagag
     3961 tccaaaaaat ctctcgttga ttttttggaa tatttgatag aaaagcaaaa aagagaggac
     4021 agggattaaa gatttacccc ttgattagct tatctccaga aagtatcgaa aaaatagaga
     4081 gtttttcttc ctcccttccc cggataaata gtgaaatcga agcgcttaaa ttagcagaaa
     4141 aggagggcat aattgttgag ttttatgact tgcgaggatt taagggagtt ctagcccaac
     4201 gccggggaca aacttttctg ggagtgaatc aaaggttgcg agaagcagaa gaagtgaaaa
     4261 ccattcttca cgagttagcc cactatttct ttcactggaa taatcgattt cgtcccatat
     4321 ttctctgcca ggagtacacc ataaaccgcg aagaaaagga agctgatttc tttgcctggt
     4381 gtcttgtgga ggaatgctgg cgggaaaccc tctcctggaa attctgggcc agagacagag
     4441 atttctctta agctcataaa taagccggga caggcagccc gagagcaacc catggcgcca
     4501 tatcattctg tcactgcaag catggatagc ctaacggttg accgaacctt gatactttcc
     4561 cctggttttt taccttttct gatatttgaa acccagtaaa tacaggcatt cacattttat
     4621 tcttctaaaa gcgtagtggc aaagcaacta cacagcctat ccttcagaca ataaaatggt
     4681 cgctctttaa atgactgaaa agcttggcct aaacactcca atacattctt gccttgtttt
     4741 ctcactgtag agataaacca cggatacgac aaaagtaatt agctcctttc tcaagaacga
     4801 aaacaacctg atatcttctc ttcgctttgg ccatacgtag gcccctctct gccaggttat
     4861 catcaaaaga aacctgaaag ccctccataa accggagagc atctttttag tacttgatga
     4921 gcctatccag taaattctta gctttactct gtttctttct acctcttc
//
LOCUS       DAOPSS010000117         4891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_13998_length_4891_cov_9.593052, whole genome shotgun sequence.
ACCESSION   DAOPSS010000117 DAOPSS010000000
VERSION     DAOPSS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13998_length_4891_cov_9.593052"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKN31_06830"
     CDS             complement(<1..427)
                     /locus_tag="PKN31_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOA99673.1"
                     /translation="MKKILIKGGTLVFPQKGVFQDVDILLESGLITRIGKGIEDSRAH
                     LINASNLLVGPGFINIHVHLREPGRETEENLLTGSRAAARGGFTSVVSMPNTHPPIDN
                     PILVRYLLLRAKEIGLVNIFPAGAITVGLEGKEMAEYGLL"
     gene            complement(424..1350)
                     /locus_tag="PKN31_06835"
     CDS             complement(424..1350)
                     /locus_tag="PKN31_06835"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOA99674.1"
                     /translation="MKWKHKDLLGINLLSKEEIEYLLERGKYFREFLDCSPKKMKYGE
                     GYCLVNFFLEPSTRTRVSFETAGRLLGMEVVNFTSEASSFQKGESLEDTAKTLSRMKF
                     DVLVVRHHQSGVAEYLAQLLPLHVINAGDGMREHPTQALLDLLTLQRKFGYFQGLKVA
                     IVGDILHSRVARSLTLGLKKLGAEVFFSGPPTLLPLAMEHLGAKIIFPVEEAVKGVDV
                     IYLLRIQKERQEAGYFPSIREYAHFFGLKEKWLDRENSRPLIMHPGPVNRGVEIGYSF
                     TEEDFSLIEEQVTCGVAVRMATLETLLQGEEQ"
     gene            2391..4454
                     /locus_tag="PKN31_06840"
     CDS             2391..4454
                     /locus_tag="PKN31_06840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOA99675.1"
                     /translation="MLKRYSFSSGLIILTTLVFVFTVSIFGYLGYHSSIQHSIAQAFD
                     TMQTIVRLTQQNISQAMEERTSDLLLLAKMLKENKVEPYLINHLFQSFDDAILVLNRD
                     GIVIKAWPESREALIGENLSHREYFQKTRESLKPYLGGHIKSILDTNIVSCSIPVIEN
                     GEFQGAIAGGMTLEQGKIRQFVSSTQFLKTGRITILDQNNNILLSTNSENIGKTFPTL
                     PLENQSPATQRLQGKDYLIWMEDIPHSSWKAVASIQKEEILSYSQRTLKIGIILGILI
                     LAITILFLYLLLRKLSQPLSLLSSIAQEYARGDYSLSPLVGGPQEIKQLSSVLDYMRQ
                     AIEEREMLLLKEQTYLKTLLQQMNQGVLVLSPEGKLKFINSRLIELTEYSEEDLIGKD
                     IGELFPSGEKEKIPYLCKWDSSRDKKTERSYLLSSEGKIIPVLLSTSPLTIKEEIMGT
                     LMVITNLQKIEEKEEELEQALEEIKLLNQELEERTQELEIVLAKLDLKLMETDQEKET
                     AQKMAITDSLTQLFNRGFFEKKFSQEFEAAQSDNHPLSLVIVDIDLFKKINDTYGHLA
                     GDKILQDLGKILTSSVREEDLVFRFGGEEFIIILHEEKEEAQKVAERIRQKVEDFSRK
                     GNEGPEKFTISLGVASYPEDAQNARELLIKADQALYQAKKQGRNRVVSYQDDFFSSSP
                     SHIID"
     gene            complement(4458..>4891)
                     /locus_tag="PKN31_06845"
     CDS             complement(4458..>4891)
                     /locus_tag="PKN31_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOA99676.1"
                     /translation="FGLSYFHERSALLQAAFEGVAFRIRSIYDLLQEVMGKPHRVVIS
                     GGFASSDKGQRVLCDVLGAPVELSSYPSAPAKGAFLLSCKLRGKVSSLNELPASFFPS
                     YPVIEPDSEAREFYKEFYEFYQKVYWKNQELFQEFSRFRLA"
BASE COUNT         1524 a         1061 c          869 g         1437 t
ORIGIN      
        1 tcaacagtcc atattctgcc atttcttttc cttctaagcc tacagttatc gctccggcag
       61 gaaaaatatt aaccagtcct atctcttttg ctcgaaggag aagataacga actaaaatag
      121 gattatcaat ggggggatgc gtattaggca tcgagactac tgacgtgaat cctcctcgtg
      181 ctgcagctcg acttccggta agaagatttt cctcagtttc tctccctggc tctcgtaaat
      241 gaacatggat gttgataaaa cctggaccca ccaggagatt agaagcatta atgaggtgag
      301 cccgggaatc ttctatacct tttcctattc gagttattaa cccactttcc agtaatatat
      361 cgacatcctg gaaaacccct ttttggggaa aaaccagagt tccaccttta attagaattt
      421 ttttcattgt tcttctcctt gaagcaaagt ttccaaagtc gccattctta cagccactcc
      481 acaggttacc tgttcttcta tcagagaaaa gtcctcttcc gtgaaactat aaccaatctc
      541 taccccccgg ttcaccggtc cgggatgcat aataagtggc ctactatttt ccctgtccag
      601 ccatttttct ttcaaaccaa aaaaatgagc atattcccgg atactgggaa aatagccagc
      661 ttcctgtctt tctttttgaa tacgcaaaag atatattaca tcaacccctt taactgcttc
      721 ttctacaggg aaaattattt tagctcccag gtgttccatc gccagaggaa gaagagtggg
      781 agggccagag aaaaaaacct ctgctcctaa ttttttcaaa cctaaagtaa gagagcgagc
      841 cacccgactg tgcaaaatat cccctactat agctactttt aatccttgaa agtatccaaa
      901 ttttctctgt aaagtcaaaa ggtcaagaag agcctgggtg ggatgttctc gcatcccatc
      961 accagcattt atcacatgaa ggggtaaaag ttgagccaga tattccgcta ctccactttg
     1021 gtgatgacgc accactaaaa catcaaactt catccgggaa agagttttag ctgtatcttc
     1081 taagctttct cccttctgga aactacttgc ttcactggta aagtttacaa cctccatccc
     1141 caggagccgg ccggctgtct caaatgaaac tcgagttcga gtactgggtt ctaagaaaaa
     1201 attaaccaga cagtaacctt ctccatattt cattttttta ggggagcaat ctaagaattc
     1261 ccgaaaatat tttcctctct ccaggagata ttcaatctct tctttactta aaagattgat
     1321 tcccagaaga tctttgtgct tccatttcac ttctctaacc ctccttatta aaaatcaagt
     1381 aagagggaca attgactatt cttaccacct cttgcctcaa aatgtgattt tatattccat
     1441 agctcattaa cctgataaat ctatcaatga gatttcagaa cttgagaggt attatcactt
     1501 cctctctgct gtgatagaga ttttcactta tttttgacaa tccagccatg ttccccggtg
     1561 gaggatggta tctttttgat attttctcat acttatcata gaagaaaaat agtcatagtt
     1621 tttgccaaga acacacaaaa acccctcgcc agatacgaga ggtttctacc atttttgaac
     1681 ttctctttct ccactttcaa aaccctgaac taggatactc ataaattatt cctctgtcaa
     1741 tcaatcgcca gaaaatccga actttattac agttaaccac agaaactgct gtaacaggta
     1801 taatagagtg ttttccttcc agtataatct ttactttttt cctttttatg tcgtagattg
     1861 tcgttattgc gagaattgtt tgtgccgcat tccgtgataa tctcgtcttt catttttttc
     1921 tcagaaagag gagagaaacc gctctcactc tcaacccctc tctacacttt cttcctccgg
     1981 aaggagttcc ccccttatgt tgaggaagag ctttttcttt tttgcttatg cttctgcctt
     2041 taatattaca tcgttgattg ttctattttt tgtttttagc gctacagact gctaattttt
     2101 tctcttttgg gattaggggt aaccgccctt tataagttat ttgatacccc cttaacatac
     2161 aatgacgaaa aagaaaagtt gagactcaaa aaagcgagat tacttcatcg cttactcctc
     2221 acaaatccag ataagaaagg agtgataggg ggcttacctt ctatagttcg gggttatgag
     2281 ggagggaaaa ctttttttat agttctgctc tctaccgtaa tcatgataac aaaaaagcta
     2341 aatttataag gaaatatcta tctttttgta atttatgata aaatctcttt atgctaaaaa
     2401 ggtattcttt ctcttccggt ttaataattt taactacttt ggtttttgtt tttactgtat
     2461 ctatttttgg ctatttgggg taccattcct ctatccagca ctctatcgct caagctttcg
     2521 acactatgca aaccatcgtt cgcctcactc agcaaaatat ctcccaggct atggaagaaa
     2581 gaacaagcga tcttttatta ttggctaaaa tgttaaaaga aaataaagta gagccttatc
     2641 tgataaatca cttgttccaa tcttttgatg acgctatatt agtgcttaat cgagatggaa
     2701 tagtaataaa agcctggccg gagagccgag aagcgctaat cggagagaac ctcagtcata
     2761 gagaatattt ccaaaaaact cgagaatcct taaaacctta tctgggcggt cacataaaat
     2821 ccatattaga caccaacatt gtatcctgta gcattcctgt aatagaaaat ggggaatttc
     2881 agggagcaat agcaggggga atgactttag aacagggaaa aatcaggcaa tttgtatctt
     2941 ccactcaatt ccttaagacc ggaagaatca ccatcctgga ccagaataat aacattttac
     3001 tctctaccaa ttccgaaaac atcgggaaaa ctttccctac tcttccttta gaaaatcagt
     3061 ctcctgcaac acaacgtctt cagggaaaag attacttgat atggatggaa gacatccccc
     3121 actcttcctg gaaagcagta gccagtatcc agaaggaaga aattttaagc tattctcaaa
     3181 gaactttaaa aattggtata attttaggaa tattaattct ggctattact attttgttcc
     3241 tttatctact attgaggaaa ttatctcaac ccctttcctt gctttcttct atagctcaag
     3301 aatacgcccg gggagattat tctctttctc ctcttgtagg tggccctcaa gaaataaagc
     3361 agctatcaag tgtcttagac tatatgcgcc aggccataga agaaagagaa atgttgcttc
     3421 tcaaggaaca aacttatctc aaaaccttgc tccagcaaat gaatcaaggg gtgctggttt
     3481 taagtccaga aggaaagttg aaatttatca actcccggtt gatagaactc actgaatatt
     3541 ctgaagaaga cctaatcgga aaagatatcg gcgaactgtt cccctctgga gaaaaagaaa
     3601 aaatacccta tctctgtaaa tgggatagca gtagagataa aaagacggaa aggtcttacc
     3661 tcttatcttc agagggtaag ataattcctg tacttctttc caccagtccc ttgactataa
     3721 aggaagaaat aatgggaacc ctgatggtaa ttaccaacct ccagaaaata gaggaaaaag
     3781 aagaagagct ggaacaagct ttagaagaga taaaacttct caaccaggag ttagaagagc
     3841 gaactcagga attggaaatc gtcctggcca aattagacct gaagcttatg gaaacagacc
     3901 aggaaaaaga aaccgctcaa aaaatggcta ttactgactc tctcacccag ctttttaacc
     3961 ggggattttt cgaaaaaaag ttttctcagg aatttgaagc tgctcaatca gataatcatc
     4021 ctctttcttt agtcatagtt gatattgacc tgttcaaaaa aataaatgat acctatggtc
     4081 acctcgccgg agataaaatt ctccaggact tagggaaaat actaacatcc agtgttagag
     4141 aagaagacct ggtatttcgt tttggaggag aagaattcat tatcatactg cacgaagaaa
     4201 aagaagaagc tcaaaaagtg gcggaaagaa tacgccagaa ggtagaggat ttctctcgaa
     4261 aaggcaacga agggccggaa aaattcacta ttagtttagg tgttgcctcg taccccgagg
     4321 atgcccaaaa cgcaagagag ctattaatca aagccgacca agccctttac caggcaaaaa
     4381 agcaaggcag aaatcgagta gtttcttatc aagacgactt tttttcttct tcaccttccc
     4441 acatcattga ctaactttca agctaagcga aaccgagaaa attcctggaa aagttcttga
     4501 tttttccagt acactttctg atagaactca taaaattcct tataaaactc ccgagcttca
     4561 gagtctggct ctataaccgg ataagaaggg aaaaaagagg ctggtagttc attcagggaa
     4621 gaaactttcc cccggagttt acaagacaga agaaacgctc ctttggccgg agctgaagga
     4681 taactactca gctcaaccgg tgctcctaaa acatcacata atactctttg tcctttatct
     4741 gaagaggcaa atcctccact tattactacc cggtgaggct ttcccattac ctcttgcaac
     4801 aaatcataaa tagagcgaat acgaaatgct actccttcaa aagcagcctg aagaagagct
     4861 gacctttcgt gaaaatagga taacccgaat a
//
LOCUS       DAOPSS010000118         4807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_14304_length_4807_cov_6.559343, whole genome shotgun sequence.
ACCESSION   DAOPSS010000118 DAOPSS010000000
VERSION     DAOPSS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14304_length_4807_cov_6.559343"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PKN31_06850"
     CDS             <1..1175
                     /locus_tag="PKN31_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOA99677.1"
                     /translation="SIPYGVLQLRGEANIAISGDEEKPLWQGEVEGEGEIWREGKKYA
                     DFGEVRGIIRGEEVQLRGGNLHIAGLDWAIEGVVSREESEIHLSGGGKLEIPEENMGL
                     SRIESDIVIKACDGKINLQGEAKIFDGWGDFTKVGDTRYYSSLLPQLEEKLEELSLSL
                     ELTLYTANKVRVKTRFLDLEMEGRLKIWGGSGRINGEGKLEVVEGNYDLVLRKIPIRG
                     YITFSPFYGLNPRVNLYGEEEVGGYHIFLEAMGPVDNYKLTLKSEPLLKEEEILSLLF
                     LGEKDGYLSLNDLDWQPLLWKLGQSLLGENFFFRSRFLGGIDIKFLDSRNYGFHGIRW
                     EQGIGENCFIGYTQDLSGKGNSFWNFEINFDKEWSLKMEGDAEGEIGWMLEFNTKF"
     gene            1196..2875
                     /locus_tag="PKN31_06855"
     CDS             1196..2875
                     /locus_tag="PKN31_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOA99678.1"
                     /translation="MVQKKIKRLLCGLVFLLALGIGGTIYAQTISPPVVAIRLEGNEN
                     VNTQLILSAISTSLKEPYNPEKVQADVKAIYDLGYFSKVWIEIKEYPDGIELIFKVQE
                     FPQVKEINISGNTALSYDEIRKVMILSPGQIMNWKIFQHDLERIKALYTDNGFLITAV
                     EEINFSEEGVLSFTIKEGIVEAVEFEGLEKTKEYVLRRELTFEPPLVFDFSKIQESLR
                     AIYNLGFFEDVSMKLEPGSDRDHVIVKVKVEEKLTGEAGLGVGYNTERGWLGYIRYQE
                     SNLGGNAQRVELRYEYGTRTLYRFLFEEPWLFNEPIYFNLAAYDQVEEKKEWVNHEVV
                     GKYEEERVGGYITLGGKIREDWGWRLRYKSEDVDINVLEGNPPDGGGKTNSLTPIVFY
                     DTRDVIDNPHEGWYGTLQAELAGGFLGGDHDYNKYILDLRNYIETGEDTVLALRFLGG
                     IADTELPSYEKFSVGGVSTLRGYDLFEFEGDKMLIFNLEYRWEVSEGTQLVFFGDAGY
                     AWPLFHDISWDEIKTGYGVGVRFDTPIGPVRLDYGIGEAGGQTYFSIGQTF"
     gene            2912..3433
                     /locus_tag="PKN31_06860"
     CDS             2912..3433
                     /locus_tag="PKN31_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOA99679.1"
                     /translation="MRRSNSLVGLILIAIAILVIAIPGWAAEKATVGLIDINKILDAH
                     PNTQKILDAEKKLQEEMEKRQTELNEKGKGKTREEVQKLEEEMNAEWAPVRDQILKER
                     QDLINQRYSDVIAAVKKVAESQKLTLVIRSELRIPVSQNELLEMPIVFYGGIDITDQV
                     IEEIKNIVAAPGE"
     gene            3440..4462
                     /gene="lpxD"
                     /locus_tag="PKN31_06865"
     CDS             3440..4462
                     /gene="lpxD"
                     /locus_tag="PKN31_06865"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOA99680.1"
                     /translation="MKLKEIAELVGGSLIGDPHWEVKGIKEPGEAQEDDLILFYHFPY
                     LEKVLFSSAKAVITKPGMEGKLKGKNVILVDNPRYAFSKLVPYFFPSYFPEKGVHPQA
                     FIHPQAFLGKNVSIGPFVVAEEGATIGDDTVIFPQVYLGRNTQVGKGCIFYPQVVVRE
                     NCWIGDRVILHSGVVIGADGFGYERAKNNYYKIPQVGKVVIEEDVEIGANSTVDRATL
                     GVTRIGAGSKIDNLVMIAHNVKIGKNVVIVAQSGVAGSTEVGDDVVMGAQSGIIDHRR
                     VGKGVQMAARTVVTTNIPDDVIISGFPAQEHKKELKEKALIRKLPELWEKIKNLEKTV
                     SKQVKR"
     gene            4459..>4807
                     /locus_tag="PKN31_06870"
     CDS             4459..>4807
                     /locus_tag="PKN31_06870"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOA99681.1"
                     /translation="MSSVVLQRTLASPCTVEGIGLHSGKSVKVTLLPASENSGIFFRV
                     GKTIIPATIQHVEDTPRCTTLKKGEKQISTIEHFLAACWGSKIDNLEVEVEGEELPGG
                     DGSSIFWFNIFREA"
BASE COUNT         1511 a          672 c         1258 g         1366 t
ORIGIN      
        1 tttctattcc ttatggagtg ctacaattga gaggagaagc caatattgct atttccggtg
       61 atgaggaaaa acctctctgg caaggagaag tggagggaga gggtgagatt tggcgagagg
      121 ggaaaaaata tgctgacttt ggtgaagtaa gaggaataat aagaggggaa gaagttcagc
      181 tcaggggtgg aaacttacac attgccggtc ttgattgggc aatagaggga gttgtttctc
      241 gagaagagtc tgaaattcat ctttcaggag gcggaaaatt agaaatacca gaagagaata
      301 tggggctatc caggatcgag agtgacattg taattaaggc ctgtgacggg aaaataaatc
      361 tccaggggga agcgaaaatt ttcgatgggt ggggagattt cacaaaagta ggggacacca
      421 ggtattactc ttctcttctt cctcaactgg aagagaaatt agaagaacta tctctttctt
      481 tggaacttac cctttatact gctaataaag taagagtaaa aactcgcttt ttagatttgg
      541 agatggaagg aagactcaaa atttggggag gaagcgggag aatcaacggg gagggtaagc
      601 tagaagtggt ggaaggaaac tatgatttgg tgttgagaaa aattcccatt agaggatata
      661 tcacctttag tcctttttat gggttgaatc ctcgagtaaa tttgtatgga gaagaggagg
      721 taggaggtta tcatattttt ctggaggcta tgggtcctgt agacaattat aagttaactt
      781 tgaagtcaga acccctcctt aaagaggagg aaatcctttc tttgcttttt ctgggggaaa
      841 aagatggcta tctttccttg aatgatcttg attggcaacc tcttctctgg aaattgggac
      901 aatctttact gggagagaat tttttcttca ggagtagatt tttgggtgga atagatataa
      961 aatttcttga ttcaagaaat tatggttttc atggtataag atgggaacag ggtattggtg
     1021 aaaattgctt tataggttat actcaagact tgagtggtaa gggaaattct ttctggaatt
     1081 tcgagataaa ctttgataaa gaatggtccc tgaaaatgga aggagatgct gaaggagaaa
     1141 ttggctggat gctggaattt aacactaaat tttgacagtg gaggagggtt gtaaattggt
     1201 tcagaaaaag attaaaagat tactttgtgg attggtgttt ttgttagcat tgggaatcgg
     1261 gggaacaatt tatgctcaaa ctatttctcc accggtagta gctattcgat tagagggcaa
     1321 tgaaaatgtt aatactcagt tgattttatc tgccatatct acctctctta aggaacctta
     1381 taatcccgaa aaagtgcagg cagacgttaa ggctatttat gatctgggat atttctctaa
     1441 agtgtggata gaaatcaaag aataccctga tggtatagag ctgattttta aggtgcaaga
     1501 gtttcctcag gttaaagaaa ttaacatttc tggtaacacc gccttaagtt atgatgaaat
     1561 tagaaaagtt atgattctat ctccaggtca gattatgaat tggaaaatat ttcaacatga
     1621 tttggagcgc ataaaagcgc tttatactga taatggtttt ttaataacag cagtagaaga
     1681 aatcaatttt agcgaagaag gagttctaag ttttaccatt aaagaaggaa tagtggaggc
     1741 ggtggaattt gaaggcttag aaaaaaccaa agaatatgtt ttacgccgag aattgacttt
     1801 tgagcctccc ctggtttttg acttttctaa aattcaagag agcttgagag ccatttataa
     1861 cctgggcttt tttgaggatg tatctatgaa gctggagccc ggtagtgatc gagaccatgt
     1921 gatagtcaag gtcaaagtgg aagaaaagct tactggtgag gcaggattgg gagtaggtta
     1981 taatactgaa agaggctggt taggttacat tcgttatcag gaatctaact taggaggaaa
     2041 tgcacaacga gtggagctaa gatatgaata tggtactcgc actctttatc gtttcttatt
     2101 tgaagagccc tggcttttta atgaacccat ttattttaat ctggctgcat atgatcaggt
     2161 agaggagaag aaagaatggg taaatcatga ggtagtcggt aagtatgaag aagaaagggt
     2221 gggaggatat attactttag gaggaaaaat aagggaagat tggggatggc gactacgcta
     2281 taaatctgag gatgtggaca ttaatgtttt agaaggtaac cctcccgatg gaggaggaaa
     2341 aactaattct ctaactccta tagtgtttta tgatactcga gatgtaatag ataaccccca
     2401 cgaaggttgg tatggaacgt tacaggcgga gttagcaggg ggattcttag gcggtgacca
     2461 tgattataat aaatacattt tagacttaag aaattatatt gaaacaggag aagatacggt
     2521 tttggcttta cgctttttgg ggggcatagc cgatactgaa ttacccagtt acgagaagtt
     2581 ttccgtagga ggagtaagta ccttgcgggg ttatgatttg ttcgagtttg aaggagataa
     2641 aatgctgatt tttaatctgg agtaccgctg ggaggtttct gaaggtacgc agctggtatt
     2701 ttttggtgat gcgggttatg cctggcctct ttttcatgat ataagctggg atgagattaa
     2761 aactggttat ggagtaggag taaggtttga cactcccatt gggccagtgc gtctggacta
     2821 tggtattgga gaagctggtg ggcaaaccta ctttagtatt gggcagactt tttgagagaa
     2881 atttaaaaca caatagaaag gaagaagatc gatgcgaaga agcaactctt tagtcgggtt
     2941 aattttgata gcaatagcga ttttggtgat tgctattcct ggttgggcag cggaaaaagc
     3001 cacggtgggt ttgatagata tcaacaagat tcttgatgct catcctaata ctcagaaaat
     3061 attagatgcg gagaaaaaac tacaggaaga aatggaaaaa agacaaaccg agttgaatga
     3121 gaaaggaaag ggtaaaactc gggaagaggt acagaaatta gaagaagaaa tgaatgctga
     3181 atgggctcca gttagagatc aaattttgaa agagcggcag gatttgataa accagaggta
     3241 tagcgatgta attgcagcgg ttaaaaaagt ggccgagtct caaaaactta ctctggttat
     3301 tcggagtgaa ttgcgtatac ccgtttccca aaatgagcta ttggaaatgc ctattgtttt
     3361 ttatggagga atagatatca ctgaccaggt gatagaggaa attaaaaata tagttgctgc
     3421 tccaggagaa tagaaggctt tgaaactgaa ggagattgcc gaattagtgg ggggaagctt
     3481 aataggtgac ccccactggg aagtaaaagg cataaaagaa ccaggagagg cacaagagga
     3541 tgatttaatt cttttttacc atttccccta tttggagaaa gtgctttttt cttctgctaa
     3601 ggcggtgata actaagccgg ggatggaagg taagctaaaa ggcaaaaatg tgatattggt
     3661 agataatcct cgttacgctt tttctaagtt ggttccttac tttttcccct cctattttcc
     3721 ggaaaaaggg gttcatcctc aagcttttat tcaccctcaa gctttcctgg gtaaaaatgt
     3781 ttcgataggt ccttttgttg tagcagaaga aggagcaacc ataggagatg atactgttat
     3841 ttttcctcag gtttacctag gaagaaatac tcaggtgggt aaagggtgta ttttttaccc
     3901 tcaggtagtg gtgagagaaa attgttggat tggagatcgg gtaattcttc acagtggagt
     3961 agtaataggg gcagatggtt ttggttacga gagagcgaaa aacaattatt acaaaattcc
     4021 tcaagtagga aaggtagtaa ttgaagagga tgtagaaata ggggcgaatt ctactgtgga
     4081 tcgagctact ctgggagtaa ccagaattgg cgctggttct aagattgaca atctggttat
     4141 gattgctcac aacgtgaaga taggtaaaaa cgtggttata gtagctcaat cgggtgtggc
     4201 aggaagcaca gaagtgggag acgatgtagt aatgggagct caaagtggga taatagacca
     4261 tcgtcgagtg ggtaagggag tacagatggc cgctcgaacg gtggtaacaa ctaatattcc
     4321 cgatgatgta attatttctg gttttcctgc tcaagaacat aaaaaagagt taaaagaaaa
     4381 ggctctaatc aggaagcttc ctgagctctg ggaaaaaata aaaaatctgg agaagacggt
     4441 aagtaagcag gttaaaagat gagttcagtg gttttacaaa gaactctggc ttctccttgc
     4501 actgttgaag ggataggctt gcatagtgga aaatcggtaa aggtcactct tctccctgct
     4561 tctgaaaata gtggtatttt cttcagagtt gggaaaacaa ttattcctgc taccatacaa
     4621 catgtggaag acactcctcg ttgcacgacg cttaagaagg gggaaaagca aatatctaca
     4681 atagaacact ttcttgctgc ttgctggggc agcaagatag ataatctaga agtggaagta
     4741 gagggggaag aacttcctgg aggggacggg agttctattt tctggtttaa tattttccgg
     4801 gaagctg
//
LOCUS       DAOPSS010000119         4800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_14331_length_4800_cov_8.831191, whole genome shotgun sequence.
ACCESSION   DAOPSS010000119 DAOPSS010000000
VERSION     DAOPSS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14331_length_4800_cov_8.831191"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1355
                     /locus_tag="PKN31_06875"
     CDS             33..1355
                     /locus_tag="PKN31_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOA99682.1"
                     /translation="MRTTPFWDEYYELLPREELEKIQLSRLKKILIRAYHNLPYYRKK
                     LEERKINPYDIKSLSCLSHLPFTTKEDLRQAYPFGAIAEPLRNVVRVHSSSGTTGTAT
                     VVGYTKKDIESWAELAARALSSYGVDEEDIIQVSFGYGLFTGGLGIHYGAEKLGATVV
                     PASAGNTLRQIQIMKDLKITVLACTPSYALYLAEVARENNINWKESSLRIGVFGAEPW
                     SEEMREDIEKKLPIRAFDIYGLSEVIGPGVAFECEEREGLHISEDHFLPEIIDPQTGR
                     VLPPGEEGELVFTTLTKEGTPIVRYRTGDISSLNYEPCSCGRTLVRMKRVFSRTDDML
                     IIRGVNVYPSQIESIVLSFDGVEPYYQIVLTRENFLDQIEIQVEVTPQFFSDQIKVLE
                     GLKKQIEEKLKTELNLSCEVKLLEPKSIARSEGKSKRVIDLRKKEEIL"
     gene            1352..1768
                     /locus_tag="PKN31_06880"
     CDS             1352..1768
                     /locus_tag="PKN31_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HOA99683.1"
                     /translation="MKQISVFLENRPGSLYEVLYKLKEWGVNLRALSLADTAEFGVLR
                     FIAEDPEEIVEKLKIENFAATLTEVLAVGVEDHPGGLAEVVGILTHQNIPIEYLYAFV
                     SPQGGKAYVVLRVEKLEEAEKVLREKHIPILEKITI"
     gene            complement(1999..2394)
                     /locus_tag="PKN31_06885"
     CDS             complement(1999..2394)
                     /locus_tag="PKN31_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOA99684.1"
                     /translation="MDTISFMELCKTGTLVDIQNAIQSGADVNALDNHLRTPLMFAAR
                     FNPHPEVISFLIQSGADVNAKDRAGWTPLMFAARFSTSPEVVKVLLEKGANAKMVDKS
                     GKKAIDYARENPALQGTEVLSQLEEMGKG"
     gene            complement(2689..3180)
                     /locus_tag="PKN31_06890"
     CDS             complement(2689..3180)
                     /locus_tag="PKN31_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOA99685.1"
                     /translation="MLSKKLEGAVNEQIKNELYSAYLYLAMAAHCEHKNFKGFANWLK
                     IQAKEEVAHAIRLYDFVNDRGGKVVLSAIDQPPAEYQSLTEIFEKVLNHEKGVTTKIN
                     HLYELAKEENDYPLQVHLQWFIDEQVEEEKNPAEILAMLQLIGESGSGIMMLDHELGE
                     RKD"
     gene            complement(3198..4397)
                     /locus_tag="PKN31_06895"
     CDS             complement(3198..4397)
                     /locus_tag="PKN31_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOA99686.1"
                     /translation="MPLVEIKPDIYWIGVNDHTTDLFEGLWPIDEEGVSYNSYLVKDE
                     KIAIVDLAKSLKVDEFLSHVKELVDFSHIDYIILNHLEPDHTGVLNILWKLSPRATIV
                     CSPLAEEMVSSFYGIRERIKVVEDGEEISLGEKTLKFFLTPMVHWPETMMTYEMNSRT
                     LFSCDAFGGYGSLQGAIFDDECSDFEFYRKESLRYYVNIVAKFSPMVIKAIDKLSSLA
                     VDLVAPSHGLIWRKNPSLIVDLYRQWASYAETGGEKGVTLVYGSMYGNTEAMMDAVAR
                     GVAESGVAVEIFDVRRTNPSYILPSLWVNKGVIVGAPTYETGLFPLMVHLLDLVIRKG
                     IKHKKTLFFGSYGWSGGGKREWEKMVSPLNWEIVDSLEFKGGAHSEVLAQGRDLGKKF
                     GEVIKNL"
BASE COUNT         1490 a         1078 c          884 g         1348 t
ORIGIN      
        1 caataaagtg gtaaaatata aactaaaaag aggtgagaac aacgccattc tgggatgaat
       61 actacgagct ccttccccgg gaggagctgg aaaaaattca gcttagcaga cttaaaaaaa
      121 tacttatccg cgcttatcac aatctgccct attaccgtaa aaaactggag gaaagaaaaa
      181 ttaatcctta cgatattaag tctttatctt gcctttccca tctacctttc actaccaaag
      241 aggaccttcg tcaagcttac cctttcggag ctatagctga gcccttgcgc aatgtagtgc
      301 gggttcattc ttcctcaggc accacaggaa cagcaacagt agtagggtac actaaaaaag
      361 atatagaatc ctgggcggag cttgctgccc gagcactttc cagttatgga gtagatgagg
      421 aagatatcat ccaggtctct ttcgggtatg gtctttttac tggagggtta gggattcact
      481 acggagcgga aaaattgggt gctactgtag ttcctgcttc tgccggtaac actcttcgcc
      541 agatccagat tatgaaagac ctgaaaataa ccgtgctggc ttgcactccc tcttatgccc
      601 tttatttggc tgaagtagcc agagaaaaca atattaactg gaaagaaagc agcctgcgca
      661 tcggggtatt tggagccgag ccctggtcag aagagatgcg agaagacata gaaaaaaaat
      721 tgcccatccg ggcttttgat atttatggac tgagcgaagt tatcggaccg ggagtggcct
      781 ttgaatgcga agaaagagaa ggattacata tctctgaaga ccactttctt cccgagataa
      841 tcgaccccca aacgggaaga gttcttcccc ccggggaaga aggagagctg gtatttacta
      901 cccttaccaa ggaaggaacc cccatagtcc gttatcgcac tggagatatc tcttctctca
      961 actacgagcc ttgttcttgt ggaagaacac tggtaagaat gaaaagagtt ttctctcgca
     1021 ccgatgatat gctcatcatt agaggagtaa atgtttatcc ttctcagatt gagagtatcg
     1081 ttttaagctt tgacggagtg gagccctatt accaaattgt ccttacccga gaaaactttt
     1141 tagatcaaat tgagattcaa gtggaagtta caccccaatt tttttctgac cagataaaag
     1201 tactggaagg attaaaaaaa caaatcgaag aaaaattgaa aactgagtta aatttaagtt
     1261 gcgaagtaaa actcttagag cctaaaagta ttgctcgtag cgaaggaaaa tctaaaaggg
     1321 ttatagatct acgaaaaaag gaggagattt tgtgaagcaa atatcagtat ttttagaaaa
     1381 caggccaggt agcctctatg aagtgcttta caaactgaaa gagtggggag taaacctgcg
     1441 agcactttcc ttagctgata ctgccgagtt cggggtcctg agatttattg cagaagatcc
     1501 ggaagaaata gtagaaaaat tgaaaataga gaattttgcc gctactttaa ccgaagttct
     1561 agccgtagga gtagaagatc acccgggggg attagccgag gtagtaggta ttcttaccca
     1621 ccaaaatatc cccatcgaat atctctatgc ctttgtttct ccccaggggg gaaaagcata
     1681 tgtagtgctg agagtagaaa agttagaaga agctgaaaag gtattgaggg aaaaacacat
     1741 tcctatttta gaaaaaatca cgatttgaaa agacatcctg cggcttaaga aaaaccacca
     1801 ggaaaaagga gagattacca cacctagata tgtatgcaat aacactccag gagaactcct
     1861 ttataacgcc ccgaaaggcg tcgttaccgt gcttctgcag ttggtgctaa atctctcctt
     1921 ttctcttttt catgtagata ctttgtttac cccaacaaaa agagcaagaa gaaaatatgc
     1981 tcgaagccac aataaatctc agccttttcc catctcttct agctgggata aaacctcagt
     2041 cccctgaaga gcagggttct ctcgggcata gtctatagct tttttcccgc tcttgtccac
     2101 cattttggcg tttgccccct tttccagaag aacttttact acttcgggag aagtactaaa
     2161 acgagcagca aacattaaag gcgtccaccc tgccctatct ttagcgttga catctgctcc
     2221 agactgaata aggaaagaaa taacttcggg atgaggatta aaacgagcgg caaacattaa
     2281 aggggtgcgt aagtggttat ctaaagcatt gacatccgct ccagactgaa tagcattctg
     2341 gatatccaca agagttccag ttttgcacaa ctccataaaa cttatagtat ccacaactcg
     2401 ccctccttaa gctgcaaatc tatcaaaaaa cacaaataaa aaagccaccg gtcctaacga
     2461 acattagcct tttcttttct caccctttcg ggtgagaaac ttcaggatgg acttccactc
     2521 aaaagcttcg agcctcccat ctttcaaaaa agaggctctt tcttccatcc tctggcctag
     2581 ttcctcagaa ccggtggtaa tcacacttta tcacctcttt tgaactttgt caacaggtca
     2641 aaattaattt tttgaaaaaa tttctaacta ctgaagacag agttcaaatc aatcttttct
     2701 ttctcccaac tcatgatcta acatcattat accgcttcca ctttctccga taagttgcaa
     2761 catagccaat atttctgcag ggttcttctc ttcttctact tgctcgtcga taaaccactg
     2821 gagatgcact tgcaaaggat agtcattttc ttctttagcc agctcgtaca aatgattaat
     2881 tttagtagta actccttttt catgatttaa gactttttca aatatctcag taagagattg
     2941 gtattctgcc ggaggttggt cgatagcact taacaccact ttccctcccc ggtcattcac
     3001 gaagtcatag agccgtatag catgagcaac ttcctcttta gcctgaatct tgagccagtt
     3061 agcaaacccc ttaaaattct tatgctcaca atgggcagcc atcgctaaat agagataagc
     3121 agagtaaagc tcgtttttaa tttgttcgtt aacagcacct tccagctttt tacttaacac
     3181 gagacattcc cccttattta tagattttta atcacttctc caaatttctt acctaaatct
     3241 ctaccttgag ctaaaacttc cgaatgagct ccacccttaa attctaaact atccactatc
     3301 tcccagttaa gaggagaaac cattttttcc cactccctct ttcctcctcc tgaccaacca
     3361 taacttccaa aaaagagggt tttcttatgt tttatcccct tacgaataac cagatctaaa
     3421 aggtgaacca taaggggaaa aagacctgtt tcataagtag gagctcctac tattactccc
     3481 ttatttaccc acagagaagg aagaatatag ctggggttgg ttcttcgaac atcaaaaatt
     3541 tctactgcta ctccactttc tgctactcct cgagccacgg catccatcat ggcttctgta
     3601 ttaccataca tagagccata aactaaagtt acaccctttt ctcctcccgt ttctgcataa
     3661 ctggcccact ggcgatacaa atctacaatc aaagagggat tttttctcca gataagacca
     3721 tgggaaggag ctactaaatc tacggccaag gaggatagtt tatcaatagc cttaatcacc
     3781 atagggctga atttagctac aatattaaca tagtagcgca gagattcctt cctgtagaat
     3841 tcaaaatcac tgcactcatc atcaaatatc gctccctgta gtgacccata acctccaaaa
     3901 gcatcacagg agaaaagagt ccgagaattc atttcatagg tcatcatagt ttctggccag
     3961 tgaaccatgg gagtgagaaa aaactttaaa gtcttctctc ccagggaaat ttcttctcca
     4021 tcttctacta ctttaatacg ttctctaata ccgtaaaatg aagaaaccat ttcctccgcg
     4081 agtggagaac atacaatagt agcccgggga gagagcttcc ataaaatatt caaaacacca
     4141 gtgtggtcag gctctaaatg atttaaaatt atataatcga tgtgagaaaa atcaaccagc
     4201 tcctttacat gacttagaaa ctcgtctacc ttcaaagatt tggctaaatc gacaatggct
     4261 attttctcgt ctttaaccaa atacgaattg taagaaactc cctcttcgtc aataggccac
     4321 aatccctcaa aaaggtcagt agtatgatcg ttaactccta tccagtaaat atccggtttt
     4381 atttcgacta aaggcataac cagaccccct tgcagtgtta tttagtatcc agctttcttg
     4441 attcagacaa aaacacctaa attgacaatt tctattacaa cccccgattt tcttttttat
     4501 tctttatcta ttagtctact catcgagtca cagaaatcag tttattaatt aaatatagta
     4561 gagctaattt tctccaagct tattcgggtg ggaaactacg gttacagttt ttcctgcaga
     4621 ctaaccaccc taaaacatcc gggcttgaga taaccgagct gtggggtata tcgtcagctt
     4681 acaggtttct tcacagatta tattcaaaat agagctcatt caccagaata gatagaggaa
     4741 gaaacctctt tcttattcaa gcttttgaga aatccaatac tagctatggt aataaaattc
//
LOCUS       DAOPSS010000120         4787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_14386_length_4787_cov_9.896027, whole genome shotgun sequence.
ACCESSION   DAOPSS010000120 DAOPSS010000000
VERSION     DAOPSS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14386_length_4787_cov_9.896027"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..>1530
                     /gene="purF"
                     /locus_tag="PKN31_06900"
     CDS             160..>1530
                     /gene="purF"
                     /locus_tag="PKN31_06900"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOA99687.1"
                     /translation="MDEKEKCGVFGVYGLKNASELTYLGLFSLQHRGQESAGIVASDG
                     KMVREKKGMGLVSEVFTSRDLKNLPGHIALGHVRYSTAGSSSPRNIQPFIGESRFGHL
                     AIAHNGNLTNALALWKKLSERGAVFQSSTDTELILHLIAQSSFDGFEDSLKDALWQIQ
                     GAFSLGILSHHQLIAVRDPWGFRPLALGKLGDGYIISSESCAFNVLGAEFVREVEPGE
                     MIIIDEKGLRSFFYAYSPRKAFCIFELIYFARPDSVVFANQVYQARKNMGRVLAEEED
                     YSADMVVPIPDSGIFAALGFSEASGIPLEFAMVRNPYVGRTFIRPGQEARELAVKIKL
                     DPIKELIRGKRIIVIEDSIVRGSTSRQRIATLKEAGAREVHMRVSSPPHIFPCHYGID
                     FPTRGELIASSKSAEEIEKYLGLDSLKYITIDGLKNSIAPPGENYCFACFNGDYPVFP
                     RERGKLI"
     assembly_gap    1531..1630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1669..2157)
                     /locus_tag="PKN31_06905"
     CDS             complement(1669..2157)
                     /locus_tag="PKN31_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOA99688.1"
                     /translation="MSQALVLYYTRTGTTEKMAQAIADTLSEEGVSTDLVDVSQFQID
                     DLTKYDLIVIGSPTYYGTMAAEIKEILDESIKLHGDLNGKIGGAFSSSANRAGGNETT
                     IWSILSALLIHGMVIKGVVEGSHYGPVAVGTFDDKAKKDCQNYARELSQLLKSLKSAP
                     AV"
     gene            complement(2172..4547)
                     /locus_tag="PKN31_06910"
     CDS             complement(2172..4547)
                     /locus_tag="PKN31_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOA99689.1"
                     /translation="MKISLNGKWRVYDEEKEFDFEGNVPGTVQGDLVNLNLMPHPYVG
                     TNEKLFRRLEEKSWIYERTFYIDKLDPKSHYDLVLEGVDTLSQVYVNGEFAGKTENMF
                     IEYRFSIKEYLREGENFLKIKIESPIEIPKTLEKNYGKLHATEETARVYIRKAQYSYG
                     WDWGARIATSGIYRNVYLECYPDARIYGSTAYLEDLDGTVVFSGYVDSIEDELDTYQV
                     EIILDGQKIDSFPVTRDIEKFKFEGKKQIKDIKLWYPNGLGESYLYSAEFKLLKNGKE
                     IYAEEKRVGFRTAKVIRANDGEGESFIFEINGKKLFAKGANWIPGENILSWLTREDYK
                     NLLQMAKDAHMNMLRIWGGGLYEDPAFYDYCDELGILVWQDFMFSCAEYPDHIDWFRK
                     IANEEARHNVIKLRSHPSIVLWCGNNENNWGFEEWDYKIKVDGKNLGNRLYLEDFPLI
                     CSQEDPSRLYWPSSPYGGERANSDRAGDRHIWEVWSGWQDYKHYCRDTSKFASEFGFQ
                     AAPDPKTIDFFASEKDKNMFSPVMLNHNKQVEGPERLLKFINNHYGLVHNFNSIVYLT
                     QLNQAEAIKTGVEHWRNRKYRTAGTLYWQLNDSWPVFSWSSIDYFKRPKALYFYTKRF
                     YNSLLPIAKNKEEQISISIINDGSYKEVDLEVELWELDGNLYKRETYRNLKVPEDSVI
                     SVGQLEVDPAILEHTVAYLGLRQNGETIIENHEIFADLRLHRPPDPKISYERAGKNLI
                     LKCEKPALGVNIRIDDENYPGENFFVLFPGREKVMKEVEGEISLKSMFDYL"
BASE COUNT         1239 a          961 c          943 g         1544 t
ORIGIN      
        1 ctgcagcgcc tggctcaaga acaaaatgtt ccttgccagg ttatagggaa aaccggaggg
       61 gagaaattag tcatcaaaaa aggagggaaa tcacttattt ccctatcagt agaagaattg
      121 aaaaatcgct gggaaggtgg tttgggagaa taaaataaag tggatgaaaa agaaaaatgc
      181 ggggtatttg gggtatacgg tctgaagaat gcttctgagt taacttatct gggtcttttt
      241 tctctccagc atcgaggaca ggaaagtgct ggtatagtag catcagatgg aaaaatggta
      301 cgggagaaaa aaggaatggg tttggtttct gaagttttca cttctcgaga ccttaaaaat
      361 cttcctggcc atattgcctt aggacacgtt cgttattcta ctgccggttc ctcttctcct
      421 cgtaatattc agcctttcat tggcgagagt cgctttggtc atttagctat tgctcacaat
      481 ggtaacttaa ccaatgctct ggctctctgg aaaaaactta gtgaaagagg ggcagtattt
      541 caatcttcca cagataccga gctcattctc cacctcatag ctcaaagttc ttttgatggc
      601 tttgaagatt ctctaaaaga tgctctgtgg caaatacagg gcgctttttc tctgggaatc
      661 ctttctcatc atcaattgat tgccgtgcga gacccctggg gttttcgccc ccttgctctg
      721 gggaaattag gtgatggcta cataataagc tcggagagct gtgccttcaa tgtattgggg
      781 gcagaatttg tgcgagaagt ggagcccgga gagatgatta taattgatga gaaagggctg
      841 cgttcctttt tctatgctta ttctcctcgt aaagcttttt gcatttttga attgatttac
      901 tttgctcgtc ctgatagtgt ggtgtttgcc aaccaggttt atcaggctcg caaaaatatg
      961 ggtagagtgt tagctgaaga agaagattac tcggcggata tggtggttcc tattcccgat
     1021 tcggggattt ttgccgctct gggtttctct gaggcatctg gtataccttt agaatttgct
     1081 atggtgcgta acccttatgt aggaagaact tttataagac cggggcagga agcgcgagag
     1141 ctggcggtaa agataaaact cgatcctatt aaggagctta ttcgaggtaa aaggataatt
     1201 gttattgaag attctatagt gcgaggaagt acttctcgcc agagaatagc taccttgaaa
     1261 gaagccggag cgcgggaggt acatatgcgg gtgagctctc ctcctcatat ctttccttgt
     1321 cattatggta tcgattttcc cacccgggga gaactgatag ccagtagtaa atctgcagaa
     1381 gagatagaaa aatatttagg gctggatagt ttgaagtata taactatcga tggtttaaag
     1441 aatagcattg ctcccccggg agagaactac tgttttgctt gtttcaatgg tgattatcca
     1501 gttttccccc gggagagagg aaaactgatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn tatcaatcag tgctaatgtt ctctttatta ttttaaaact aaacagcagg
     1681 ggctgatttt agtgatttga gaagctggga tagctctcga gcataattct ggcaatcttt
     1741 ttttgcttta tcgtcaaaag tgcccacagc taccggtcca tagtggcttc cctcaactac
     1801 cccttttatc accatcccgt gaattaaaag agcagataaa attgaccaga tggtggtttc
     1861 attacctcct gctcggttgg cggaagaaga aaaagctcct cctattttgc cgtttaagtc
     1921 accgtgaagt ttaatactct catctaagat ttctttgatt tctgccgcca ttgttccata
     1981 ataggtggga gagccgatga ctatcaaatc atatttggtt aagtcatcta tctggaattg
     2041 agaaacatca accaggtcgg tagagactcc ctcttcgctg agggtatcag caatagcttg
     2101 agccattttt tctgtggttc cagtacgagt ataataaagt actaaagctt gagacatatg
     2161 ccttcctcct tctataaata gtcaaacatg cttttcaggg aaatttcccc ttcaacttct
     2221 ttcataactt tttctctacc cgggaacaga acgaagaagt tttctccggg atagttttca
     2281 tcgtcaattc ttatatttac tcctaaagct ggtttttcac attttaatat caggtttttg
     2341 cccgctctct catagcttat tttaggatcc ggggggcggt gaagccttag gtcagcaaat
     2401 atttcgtggt tctcaataat tgtctccccg ttttgtctta agccgaggta ggctactgta
     2461 tgttctaata tggcaggatc aacttctaat tgaccgactg aaatcactga atcttcggga
     2521 accttaagat tgcgataggt ttccctttta tataaattgc catcaagttc ccacaattca
     2581 acctctaaat ctacttcttt gtaacttccg tcatttatta tggaaataga tatttgttcc
     2641 tctttgtttt tagcgatggg aagcaagcta ttgtaaaatc ttttggtgta aaaatataga
     2701 gctttgggtc ttttaaaata gtctatggac gaccagctga aaactggcca ggagtcattt
     2761 aattgccagt aaagtgtccc ggccgttctg tattttctgt ttctccagtg ttctacccct
     2821 gttttaatag cttctgcctg atttaattgg gtgagataga caatagaatt gaagttgtgc
     2881 accaggccgt agtggttatt tatgaatttt agcaatctct cgggtccttc tacttgttta
     2941 ttgtggttca gcataaccgg tgagaacatg tttttgtctt tctctgaagc aaaaaagtct
     3001 atggttttag gatccggtgc agcctggaag ccgaattcgc tcgcaaattt agaggtatcc
     3061 cggcagtagt gcttataatc ctgccagcct gaccagactt cccatatatg tctatctccg
     3121 gcccggtctg aattggctct ttcccctccg taagggctgg aaggccagta tagtcttgag
     3181 ggatcttcct gggaacaaat aagagggaag tcttctaaat acagtctgtt acctaagttt
     3241 tttccatcta cttttatttt ataatcccat tcttcaaatc cccaattgtt ttcgttattt
     3301 ccacaccaca ggacaatgct ggggtggctt ctgagtttta tgacattgtg tcttgcttct
     3361 tcgttggcta tctttctgaa ccaatctata tggtcggggt attcggcaca ggagaacatg
     3421 aaatcttgcc agactaaaat acccagctca tcacaataat catagaaagc agggtcttcg
     3481 tataagccac caccccagat tcgcagcata ttcatatgag catctttagc catctgaagt
     3541 aaatttttgt agtcttccct ggttaaccag ctcaagatgt tttctcctgg tatccagtta
     3601 gcacctttag cgaatagttt tttgccgttt atctcaaaaa tgaaactttc accttcacca
     3661 tcatttgctc ttattacctt tgccgttcta aaccccactc tcttttcctc ggcatatatt
     3721 tctttcccgt ttttgagcaa tttgaattcc gcggaataaa gatagctttc tcctaaacca
     3781 ttgggatacc acaactttat atcttttatc tgttttttcc cctcaaattt aaatttttca
     3841 atatcacgcg ttactggaaa gctatctatt ttctggccat ctaagataat ctctacctgg
     3901 taagtgtcaa gctcatcctc tatagaatca acatagccag aaaaaaccac agttccgtct
     3961 aaatcttcaa gatatgctgt ggagccataa atccgggcgt caggataaca ctccaggtaa
     4021 acattcctgt atataccgct cgtggcaatt cttgctcccc agtcccatcc gtaggaatat
     4081 tgagctttcc ttatatacac tcgagctgtt tcttcggtgg catgcagttt cccataattt
     4141 ttttctaaag tttttggtat ttctatagga gattctattt taatctttag aaaattttct
     4201 ccctccctta aatattcttt tatggaaaat ctatattcga tgaacatatt ttcggtcttc
     4261 cctgcaaatt ctccgttcac atatacctga gaaagagtat ctacaccttc taacaccaga
     4321 tcgtagtggg acttcgggtc taatttgtct atatagaaag ttctctcata aatccaggat
     4381 ttttcctcta atcgtctaaa cagcttctca tttgttccca cataaggatg aggcattaaa
     4441 ttcaaattta cgaggtctcc ttgcactgta cctggaacat taccttcaaa atcgaattcc
     4501 ttttcctcat cgtaaactct ccattttccg tttaaagaga ttttcaagaa actctcctcc
     4561 cctgtgtctg tgttataaat aaattataag ttgtaaatta tttcaggaat tggtttcagt
     4621 agttaaaaat attgtcttaa aatccctgat ttattgtacc atatcattcg atattactca
     4681 ggggagaaat tatgacagtt ctacgtcaat aagtgtgaag ttaaggtgga ccccattgtc
     4741 aagacagaaa agagactttt aaggtgtatt atttcctcct gtttgtt
//
LOCUS       DAOPSS010000121         4736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_14609_length_4736_cov_8.116001, whole genome shotgun sequence.
ACCESSION   DAOPSS010000121 DAOPSS010000000
VERSION     DAOPSS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14609_length_4736_cov_8.116001"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKN31_06915"
     CDS             <1..441
                     /locus_tag="PKN31_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99690.1"
                     /translation="ENIESLNPKFKIEVQGVQWPTYLDAYRSGQLPAFIIGWLADYPD
                     PHNFIFTYFHSNGVYGTTQGQAFIDFARENLDSLVEKAIAETDEAERQKLYEEIQTIA
                     YEDALGITLYQPLEIYPMRDWVKGWEFNPMRSGEVNWEGVYKEE"
     gene            508..1539
                     /locus_tag="PKN31_06920"
     CDS             508..1539
                     /locus_tag="PKN31_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99691.1"
                     /translation="MTFYIIRRLLFLPLTLLGISLLVFSMIGLLSPYQRLATFINSPE
                     RLKGADIDKLVELYGLNDPLPIQYGRWLKNLFQGNLGWSYVGKEPVYDALVRRFPYTL
                     ELALFSIFPIIFLGINLGVISAVNHNRPLDHGLRIFAVVGWSFPDFVFGLFILMIFYS
                     VLGWFPPGNLSLWAEEVVKSSVFHNFTGMTTIDALLNGRLDIFWDAFLHLLGPVLTLT
                     YVQFAFILRITRSSMLEVLQKDYVRTARAKGLDERVVINKHARKNALIPVATVSGQTI
                     IGLLAGAVIVETIFNRTGIGSFMSLAAQQLDYASIMGGTLFFGFILVLGNLIVDISYA
                     FIDPRVRLE"
     gene            1543..2424
                     /locus_tag="PKN31_06925"
     CDS             1543..2424
                     /locus_tag="PKN31_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99692.1"
                     /translation="MNRETRRIFRRLSSDASAILGFALLIFFVIVAILAPFIASPPPG
                     RDPYIMPIVTWSSNPLPPSPEHPFGVAGKRDIFYGVVWGTRTAFRVGIMVTLISTALG
                     LLIGSVAGYFGKLWDEILMRITDIFMAIPFLVAAMVLTTILGTGLDKVMMAMTVFSWM
                     GPARLIRGNILQVKEEQFVLAAKALGVSDFSIIIRHVLPNTIFPVLIQASMRMGSLVI
                     TAAALSFLGVGAPQGYADWGALLNYARNWMLGGQGEALKYWYMTVFPGAAMVLFVLAW
                     NLVGDALRDAFDPRLRL"
     gene            2427..3434
                     /locus_tag="PKN31_06930"
     CDS             2427..3434
                     /locus_tag="PKN31_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99693.1"
                     /translation="MEENNLLLQVQDLKVYFYNEEGQELKAVDGVGFYLRKGETIALV
                     GESGCGKSVTSLAILRLVDSDGKIQGGKILLEGRDLLSLSEREMREVRGRDISMIFQE
                     PSSALNPVYTIGDQIIEAIILHQKLSYHQARKTAEELLRLVGIPEARKRLDEYPHELS
                     GGMKQRAMIAMALSCHPKVLIADEPTTSLDVTIQAQILELIEELQRQLNMGVILITHD
                     LGVVAGMAERVVVMYAGKVVEEGSRWDIFHHPHHPYTWGLLHSIPRLDVKQEKLAIIP
                     GVVPDPLNFPSGCRFRNRCPLEDEVCLTEPSREEIENQHYVYCHHHSQIEVLETRGFS
                     Q"
     gene            3431..4423
                     /locus_tag="PKN31_06935"
     CDS             3431..4423
                     /locus_tag="PKN31_06935"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOA99694.1"
                     /translation="MNNVPLLQVEDLKKYFPIKAGVFRRTVGWVKAVDGVSFEVFPEE
                     TLGIVGESGCGKSTLGMTVMRLYEPTAGSIFFQGEDISSLPASQLKGVRRNIQMIFQD
                     PFTSLDPRMHIGSIVEEGMKVHRVASPPEVRRRALDLLEKVGLGAEYFSRYPHEFSGG
                     QRQRIGIARALALNPRLVVADEPVSSLDVSVRSQILNLLKDLKREYQLTYIFISHDLA
                     VVRQICDRVIVMYLGKVVEIADGDTFFSSPSHPYSVALTSAVPVPDPDYEVKRIILSG
                     DVPSPINPPEGCRFHTRCPVAQEVCSQVEPALEEVGENQWAACHFPGSLSWERA"
     gene            4491..>4736
                     /gene="infC"
                     /locus_tag="PKN31_06940"
     CDS             4491..>4736
                     /gene="infC"
                     /locus_tag="PKN31_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOA99695.1"
                     /translation="MSIEVKYRVNHQIGAREVRLISPEGEQLGVVPLKKALEMAEEAG
                     LDLVEVAPDAHPPVCRIMDYGKFRYEKEKKIKLSRKKQ"
BASE COUNT         1222 a          889 c         1182 g         1443 t
ORIGIN      
        1 gaaaatattg agtctctcaa tcctaagttt aaaatcgaag tgcagggagt tcagtggccc
       61 acttatctcg atgcttaccg gagcggccag cttccagcct ttatcattgg ttggttagcc
      121 gattatcccg accctcacaa ctttatattc acctatttcc acagcaatgg agtttatggc
      181 accactcaag gacaagcctt tatagacttt gctcgagaaa atctggattc attagtagaa
      241 aaagccatag ctgagacaga tgaagcagag cgacaaaaac tctatgaaga gattcagacc
      301 attgcttatg aagatgctct gggcataacc ctctatcaac ctctggaaat ttaccccatg
      361 agagattggg taaaaggctg ggaatttaac cccatgaggt caggagaagt taactgggaa
      421 ggagtgtata aagaagagta atagaaatag aagaaggggg gagaaatctc cccttctttt
      481 tgtaaagcga ggtaagatga aaagaagatg acattttata taattcgccg acttttgttt
      541 cttcctttaa ctttactggg gatttctctt ttagtctttt ccatgattgg attattgagt
      601 ccttatcaga gattggctac ttttattaat agcccggaaa ggttaaaggg agcagacatc
      661 gataaattag tggagctcta tggtctgaat gaccctctcc ctatccaata cggaagatgg
      721 ttgaaaaacc tctttcaagg taatctgggc tggtcttacg taggaaaaga accggtttac
      781 gatgcacttg tgcgacgatt tccctatact ctggagctgg ctttattttc tatctttcct
      841 atcatttttc tgggaataaa cttaggagtg atatctgcgg taaaccacaa tcgtccctta
      901 gaccatggtt tgcgcatatt tgcagtggtg ggatggtctt ttcctgattt tgtttttgga
      961 ttgtttatcc tgatgatttt ctatagtgta ttgggctggt ttcctcccgg gaatttgagc
     1021 ctgtgggcag aagaagtggt caagtcttct gttttccata attttaccgg tatgaccact
     1081 atagatgctc ttctcaatgg aagactggat attttctggg acgcctttct ccacctttta
     1141 ggcccggttt taactttgac ttatgtacaa tttgctttta ttttgcgtat tactcgctct
     1201 tctatgttag aggtgttgca gaaagattat gttcgcactg cccgggctaa aggactggat
     1261 gagcgggtag ttattaataa acatgctcgt aaaaatgctc tcatccctgt ggctacagta
     1321 agtggtcaga ctattatcgg acttttagcc ggagcagtga ttgtagagac catttttaat
     1381 cgtaccggta tcgggagttt tatgtctttg gcagctcaac agctggatta tgcttccatt
     1441 atgggaggga ctctattctt cgggtttata ctggttttag gaaacctgat agtggatatt
     1501 tcctatgcct ttattgaccc ccgggtgagg ttggagtagt tgatgaatag agaaactcga
     1561 cgcatattca ggagactatc gtccgatgct tctgctattt taggttttgc tctgcttatt
     1621 ttctttgtaa tagtggccat cctcgctccc tttattgctt cacctcctcc aggtagagac
     1681 ccctatatta tgcctatcgt tacctggagc tctaatcctc ttcctccttc tcctgagcat
     1741 ccttttgggg tggcaggaaa aagagacata ttttatggag tggtatgggg aactcgcaca
     1801 gctttccggg tgggaataat ggtaacttta atttctaccg ctttaggact tctaataggg
     1861 tcggtggccg ggtattttgg gaaactgtgg gatgaaattc tgatgagaat caccgatata
     1921 tttatggcta ttcctttttt ggtggcggct atggttttga ctaccattct ggggacagga
     1981 ttagacaagg taatgatggc tatgacagtg tttagctgga tggggccagc acggctcatt
     2041 cgagggaata tcctgcaagt taaagaagaa caattcgttt tagcagctaa agctttagga
     2101 gttagtgatt tttccatcat tatccgccat gttttaccca acaccatttt tccggtctta
     2161 attcaggctt ctatgcgcat ggggtctttg gttatcactg ccgctgcttt gagttttttg
     2221 ggagtagggg ctcctcaggg ttatgctgat tggggggcgc ttctaaatta tgctcgaaac
     2281 tggatgctgg gggggcaggg agaggctctt aagtactggt atatgaccgt gtttcccggt
     2341 gcagctatgg tgcttttcgt tctggcctgg aatttagtgg gagatgctct gcgagatgct
     2401 tttgaccctc gtctgaggtt ataaatatgg aagaaaataa tttactttta caagttcagg
     2461 atttaaaagt ttatttttat aatgaagaag ggcaggaatt gaaagcagtg gatggagtgg
     2521 gtttctacct caggaaggga gaaactatag ccctggtggg agaatcagga tgtggcaaaa
     2581 gtgtcacctc tttagctatc ctgcggctgg ttgattctga tggaaaaatc cagggaggga
     2641 agatactcct ggaaggtaga gacttacttt ctctttcaga aagagagatg cgagaagtca
     2701 gaggaagaga tatttccatg attttccagg agccatcttc ggctttaaac ccggtttata
     2761 ccataggaga tcaaataata gaagcgataa ttttgcacca gaaactttct tatcatcaag
     2821 ctcggaaaac agcagaagaa ttactcagac tggtgggtat tcccgaagcc cggaagaggc
     2881 tggatgaata tcctcatgag ctctcaggag gaatgaaaca gagagctatg attgccatgg
     2941 ctttatcctg tcatcctaaa gttttaattg ctgacgagcc tactacttct ctggatgtta
     3001 ctattcaagc tcagattctg gaattgattg aagaattgca gcgacaactt aatatggggg
     3061 ttattcttat tacccatgat ttaggagtgg tggctggtat ggcagagaga gtggtggtta
     3121 tgtatgctgg aaaggtggta gaagaaggaa gccgctggga tatattccat catccccatc
     3181 acccttatac ctgggggctt ttacattcca tacctcgcct ggatgtcaag caggaaaaat
     3241 tggccatcat tcccggggta gttcccgacc ctcttaattt tccctccggt tgcagatttc
     3301 gcaacaggtg tcctttagaa gatgaggtat gtcttactga gccttcccgg gaggagatag
     3361 agaatcaaca ttacgtatac tgtcatcatc attctcaaat tgaggtatta gaaactcggg
     3421 gttttagcca atgaacaatg ttcctttgtt gcaagtagag gatttgaaaa agtattttcc
     3481 cattaaagcg ggagttttcc ggagaaccgt ggggtgggtt aaagcagtgg atggagtaag
     3541 ctttgaggta tttcctgaag aaactttagg tatagtagga gaatcgggtt gtgggaaaag
     3601 caccctggga atgaccgtta tgcggcttta tgagccaact gcaggaagta tatttttcca
     3661 gggggaggat atttcttctc tgccggcttc tcaactgaag ggtgttcggc gaaacattca
     3721 aatgattttt caggaccctt ttacctcttt agaccctcgc atgcatatag gctctatagt
     3781 tgaagaaggc atgaaagtgc atcgtgtggc ttcccctcct gaggttcgac gcagggcact
     3841 ggatttgctg gaaaaagtgg gtctgggagc ggaatatttt tcccgttatc cccatgaatt
     3901 ttccggaggt cagaggcaga gaattggtat tgcgagagcc ttagctctta atcctcgttt
     3961 agtagtagca gatgagccgg tttcttcact ggacgtttcg gttcgttctc aaattttgaa
     4021 tttattgaaa gacttaaaaa gggaatatca actgacctat attttcattt ctcacgacct
     4081 ggcggtggtg agacagattt gtgaccgggt tattgttatg tatctgggaa aggtagtgga
     4141 aatagcggat ggagatactt tcttttcttc tccttctcat ccctatagtg tagctttgac
     4201 ttctgctgtg ccggttccag accccgatta tgaagttaaa cgaattattt tgagcgggga
     4261 tgttcccagc cctattaatc ctccggaggg gtgtcgtttc cacacccgct gtccggtggc
     4321 tcaagaggta tgctctcagg tggagcccgc tttagaggaa gtgggagaaa atcagtgggc
     4381 ggcctgtcac tttccgggta gtttgtcctg ggaaagggct taaaataatt gactaagatg
     4441 aataaatatg gtatgttttt gaaccatcta agaaagaaac tagggaggcg ataagtatag
     4501 aagtaaaata ccgcgttaac catcaaattg gagcgcggga agtacgattg atttctccgg
     4561 agggagagca gttaggagtt gttcctttaa aaaaagccct ggaaatggct gaagaagctg
     4621 gtttagacct ggtagaagta gctcctgatg ctcatcctcc tgtatgccgg ataatggact
     4681 atggaaagtt cagatatgaa aaggagaaaa aaattaaact ttcccgcaag aagcag
//
LOCUS       DAOPSS010000122         4728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_14639_length_4728_cov_8.578001, whole genome shotgun sequence.
ACCESSION   DAOPSS010000122 DAOPSS010000000
VERSION     DAOPSS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14639_length_4728_cov_8.578001"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PKN31_06945"
     CDS             <1..939
                     /locus_tag="PKN31_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOA99696.1"
                     /translation="NTREMFKKAMAGKYAVPGYNFNNMEQLQAIITACVECNSPVILQ
                     ISKGARQYANQTLLRYMVPGAVEMAREMGSNIPIALNLDHGDSFELVKSCVDSGFSNV
                     MIDGSHLPYEENVALTKKVVDYAHPRDVTVEGELGVLAGIEEHVSSEVSHYTDPAQVE
                     DFVGRTGVDSLAISIGTSHGAYKFKLKPGEPFPTLRFDILEEVERRLPGFPIVLHGAS
                     SVLKKYVDIINQYGGALEDAVGVPEDQVRRAAQSAVCKINIDSDGRLVMTAMIRKYLA
                     ENPKEFDPRKYLGYAREELKKMYISKCELLGSAGQA"
     gene            1125..1628
                     /locus_tag="PKN31_06950"
     CDS             1125..1628
                     /locus_tag="PKN31_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOA99697.1"
                     /translation="MIEVALRFSPSEILEMAKELERRGIDFYNRLAQLTSSSSAREVF
                     SFLKEEEKKHLEFFTNLQKELNEGQEILFSTTDESVNYLGAIVESGILDKVLKGVNLT
                     PAGIKMEDALEVGIEIEKESILFYQGLIPMVSPEKREWLDKVIEEEKKHFLRLQSMKR
                     ELKEKEG"
     gene            1638..2600
                     /locus_tag="PKN31_06955"
     CDS             1638..2600
                     /locus_tag="PKN31_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI/YhbO family deglycase/protease"
                     /protein_id="HOA99698.1"
                     /translation="MRRIIVLVESQYQELELWYPLLRLREAGIEAKLVGINVEETFYG
                     RHGFPARAEMAISLINARDFDGVIIPGGFAPDYLRRLPVVLNLVREFYQGGKLVAAIS
                     RGPWVLVSADIVRGKRIAGLVSIKDDVNNAGGDFTDLPVMVDGNIITAQGPDYLPFFM
                     EEVLYFLWQNKLCLDRPSPDGWLTDTTGNTVLLSQLCRGKAGVILFFDSVFSPQGSQM
                     LPSYLSLKEEIEKKKGILIPISSDTFFAQREFIEQNNLPFPLWSDSSAQVIKAFGVWD
                     RSYLARWAAFLVDRQGFLRYGEDCPGGNLDNLPGFRRKLESILG"
     gene            2614..3255
                     /locus_tag="PKN31_06960"
     CDS             2614..3255
                     /locus_tag="PKN31_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOA99699.1"
                     /translation="MFLQDKDKETLINFLKENLSREVSLKFFTQELECETCQSVRSLL
                     EEIVALSPLLHLEIYNFLLDKDKVDLYDIDKIPAIVVEGEKDYGIRFYGIPAGYEFSG
                     FIETLVWVSRGETDLRKESKDFLSSLTREVDIQTFVTVTCPYCPQAVVLGHKMAIEGE
                     KVRSAMIEVEEFPHLAYRYGVRAVPKIVINERVSFEGAVPEEYFLTKIIEAVS"
     gene            3270..4313
                     /locus_tag="PKN31_06965"
     CDS             3270..4313
                     /locus_tag="PKN31_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOA99700.1"
                     /translation="MAQTMKAAVMKGIGQIEIEELPIPIPGEEEVLVRIKSVGVCGSD
                     VHYFVEGRIGDYVVKPPFILGHECAGEVVEIGKKVKNLKPGDRVTMEPGIPCGKCEFC
                     RSGRYNLCPDVVFWATPPVDGTFCEYVTHPANFTYPIAEKVSFEEGALVEPLAVGVYA
                     THRAHAEPGEMALVLGSGPIGLVTIEALSARGVTRIIAVDIFDFRLKKAKELGANFTI
                     NAKEEDVKAQVEELTQGRGVDVVFETAGSVSTSQLTVEVTKKGGRVVLIGLPSQTHFD
                     FELLKVVDKELDVLGIFRYANVYPGCVDLLNAGKVDLQSLITHRFPLEKTQEALQFAH
                     EHKEESIKVVVNL"
BASE COUNT         1364 a          863 c         1161 g         1340 t
ORIGIN      
        1 aatactcggg aaatgttcaa aaaggctatg gctggaaaat atgccgtacc aggttataat
       61 ttcaataaca tggaacagct gcaggccatc atcactgcct gtgtagaatg taattcacca
      121 gtgattttgc aaatttccaa gggtgcccgt cagtatgcca atcaaaccct gttgcgctat
      181 atggttcctg gagcagttga gatggctcga gaaatgggaa gcaatatccc tatcgctttg
      241 aacctggatc atggagattc ttttgagtta gtaaaatcct gtgtagactc tggattttcc
      301 aatgtgatga ttgatggttc tcatcttcct tatgaggaaa atgtggctct aaccaaaaaa
      361 gtagtggact atgcccatcc tcgtgatgtg actgtagagg gagagctagg agttttagcc
      421 gggattgaag aacacgtttc ttctgaagtt tcccactata ctgacccagc ccaggtggag
      481 gattttgtgg gacggacagg ggtagatagt ttagctatct ccatcggcac ctctcacgga
      541 gcctataaat ttaagctaaa accgggcgag ccttttccca ctctccgctt tgatatttta
      601 gaagaggtgg agcgccgact tcctggtttt ccaattgtgc tccatggagc ctcttcggtc
      661 ttgaagaaat acgtagatat tatcaatcaa tatggaggag cgttggaaga tgccgttggt
      721 gtacctgaag accaggtccg tcgtgctgct caatcggcag tatgcaaaat caacatcgac
      781 tccgatgggc gattggtaat gacagctatg attcgaaaat atttagctga aaatcccaag
      841 gaattcgatc cgcgcaaata tctgggttat gcgcgggaag aacttaagaa aatgtacatt
      901 tccaaatgcg aacttctggg tagtgcgggc caagcttgat aatgattatc aaggagcaag
      961 gtatcttctg gatacttgca gcttaaggaa tagtgcctac acaagataaa tctaaaatta
     1021 agctgggtga ggcacgtaca tcaagtgatg cgcacagtga tgaaagcaat aataagtgtg
     1081 agggagatat ctccctcaca ccatagttaa atataagaag ggatgtgata gaggtggcat
     1141 tgcgtttttc tccctctgaa atcctggaaa tggctaaaga gttagaaaga aggggtatag
     1201 atttttataa ccgattagca cagttaactt cctcttcctc agcacgagaa gttttttcct
     1261 tcttaaaaga agaagaaaaa aagcacctgg aatttttcac taatcttcag aaagagctta
     1321 atgaaggcca agaaattctc ttctctacta ctgacgagag tgtcaactat ctgggggcaa
     1381 ttgtagaaag tggaattctg gacaaagtac taaaaggggt aaaccttacc cctgcaggga
     1441 taaagatgga ggatgcctta gaagtgggca tagagataga gaaagaaagt atcctttttt
     1501 atcaaggact tattcccatg gtttccccgg agaaaagaga atggttagat aaagttattg
     1561 aggaagagaa aaaacatttt ttgcgtcttc agagcatgaa gcgagagtta aaggaaaagg
     1621 agggttaaaa attggctgtg cgtcggataa tagttttggt agaaagtcaa tatcaagagt
     1681 tggagttatg gtatcctttg cttcgcttgc gagaagcggg aatagaggca aaattggtag
     1741 gaatcaacgt agaagagact ttttatggtc gtcacggttt tccggcgcgg gcagaaatgg
     1801 ctatttcttt aattaacgcc cgagattttg acggagtaat cattcccggt ggttttgctc
     1861 ctgattatct gcgtcgtctc cctgtagtgc ttaatttagt cagggaattc taccagggag
     1921 gaaaattggt ggcagctatt tctcggggtc cctgggtgct ggtctctgct gacattgtaa
     1981 gaggaaaaag gatagcaggg cttgtatcca ttaaagatga cgtgaataac gcaggaggag
     2041 attttaccga cctaccagtt atggtagatg gaaatattat taccgctcaa ggtcccgatt
     2101 atcttccttt ctttatggaa gaagtcctgt attttctgtg gcagaataaa ctttgtctgg
     2161 atagaccttc tcctgatggt tggctaactg acaccaccgg taatacagtt cttctttccc
     2221 agctctgccg gggtaaagca ggagtaattc tcttttttga ttcggttttt agccctcagg
     2281 gaagtcagat gcttcctagc tacctttctt taaaagaaga aatagagaaa aagaaaggta
     2341 ttttaatacc tataagctca gatactttct tcgctcagcg agaatttata gagcagaaca
     2401 accttccctt tcccctgtgg agcgactctt ctgcccaagt gattaaagct tttggagttt
     2461 gggatagaag ctatctggct cgatgggcag cgtttctggt cgaccgtcag ggctttttgc
     2521 gttatggtga ggactgtccc ggcgggaact tagataatct ccctggtttc cgaagaaaat
     2581 tggaaagtat attaggatag ggggtgacca attttgtttt tacaggacaa agataaagaa
     2641 actctgatta actttttaaa agaaaattta tctcgtgagg taagtctgaa gttttttact
     2701 caggaattag aatgtgaaac ttgccagagc gtgcgctcgc tcttagaaga gatagtggct
     2761 ctttctcccc ttctgcatct cgagatttat aactttcttt tagataaaga taaagtagat
     2821 ctttatgaca tcgataaaat accagctata gtggtagaag gagaaaaaga ctatggtatt
     2881 cgcttttatg ggataccagc aggatatgaa ttttctggtt ttatagagac cctggtgtgg
     2941 gtctcacgag gagaaacgga tttaaggaag gagagtaagg attttctttc ttcgcttacc
     3001 cgggaagtag atattcagac ttttgtaact gttacctgcc cttattgtcc gcaagcggtg
     3061 gttttaggac acaaaatggc tatagaggga gagaaggtac gctcagcaat gatagaggtg
     3121 gaagaatttc cccacctggc ttatcgatat ggcgtgagag cagtgcccaa aatagtcatc
     3181 aatgagcgag tgtcttttga aggagcagtt ccggaagaat atttcttaac taaaattata
     3241 gaagcagtgt cttgagagga ggaaattata tggctcaaac tatgaaagca gcagttatga
     3301 aagggattgg gcaaatagaa attgaagaat tacctattcc catccctgga gaagaagagg
     3361 tgctggtacg gataaaaagt gtgggagtat gcggctctga tgttcactac tttgtggagg
     3421 gaagaatcgg cgactacgtg gtaaagcccc cttttattct gggtcacgaa tgtgccgggg
     3481 aagtggtgga gataggaaag aaagttaaaa atttaaagcc tggagatcgg gttactatgg
     3541 aacctggtat tccctgcgga aagtgcgagt tttgccgctc cggaaggtat aacctctgcc
     3601 ctgacgttgt tttctgggct actcccccag tggatggtac tttttgtgag tatgtaactc
     3661 acccagctaa ttttacctat cctatagcag aaaaggtgag ctttgaagaa ggggcactgg
     3721 ttgagcctct ggctgtggga gtatatgcca ctcatcgggc acatgcagag ccgggagaga
     3781 tggctctggt tttaggtagt ggccctatag ggcttgtaac tatcgaagct ctttcagctc
     3841 gaggagttac ccgaataata gcggtggata tttttgactt ccgcctgaaa aaagcgaaag
     3901 aattgggagc taattttacg ataaatgcta aagaagaaga tgtaaaagct caggtagaag
     3961 aactaactca gggccgagga gtagacgtgg tttttgaaac agcgggaagt gtttccacct
     4021 ctcagctaac tgtagaggtt accaaaaaag gaggaagagt agttctcatc ggtctcccct
     4081 ctcaaactca ttttgatttt gagcttttaa aagtggtaga taaagaacta gatgttctgg
     4141 gaattttccg ttatgctaac gtttaccctg gttgtgtaga tttattgaat gccggaaaag
     4201 tagacctcca atctctgatc acccatcgtt tccccttaga gaaaactcag gaagctttgc
     4261 aatttgctca tgagcacaaa gaagaatcaa ttaaagtagt agtgaatttg tgaaaaattg
     4321 gcgggggctt gctttaaagc cccctatggt accttggaaa attcattgca atatccctct
     4381 tctcagcact ccacccctct tccttctcca gggtatctgc ggtttatctt tttgctttac
     4441 gtgacaattc cgtttctcct tttatcattc ccgaaattta gcggggaatc cgctttcttc
     4501 tgtcataaaa ccattcaagg gtgacgacaa aaggtgataa accattcaga agcaggctaa
     4561 aaacgactcg aaagttaccg gggtgatgaa aaagaagggg tacaggatgc tataattaaa
     4621 atccctcaat aagtacttct ccagaatggt ataattttca cagttattaa ccaattggga
     4681 ggtaaaaatt ttgaaaaaat gccttcgagt ggttctgggg gttttgat
//
LOCUS       DAOPSS010000123         4697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_14765_length_4697_cov_4.866006, whole genome shotgun sequence.
ACCESSION   DAOPSS010000123 DAOPSS010000000
VERSION     DAOPSS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14765_length_4697_cov_4.866006"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..594)
                     /gene="cas2"
                     /locus_tag="PKN31_06970"
     CDS             complement(331..594)
                     /gene="cas2"
                     /locus_tag="PKN31_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOA99701.1"
                     /translation="MFVIMVYDVNQKRVAKVLKIARKYLNWVQNSVLEGEITDAKYEK
                     LKVEITKTIELEEDSVIFYVLRSTRYTKREIIGLSKGGHQVII"
     gene            complement(595..1590)
                     /gene="cas1b"
                     /locus_tag="PKN31_06975"
     CDS             complement(595..1590)
                     /gene="cas1b"
                     /locus_tag="PKN31_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOA99702.1"
                     /translation="MSKSIYVFSSGKLERKHNTIYFEDSEGKRKYIPIENTRELHIFG
                     EVSLNKKILEYLSQKEILLHFYNYYGYYSGSFYPREHYNSGYLILKQAEHYLDEEKRL
                     ILAKSFVRGALQNMEKVLKYYGNRGIELSPLIEKINSFSQSAEEQNTIERLMAIEGNA
                     RQIYYSSFDLIVQKEDFTMEGRTKRPPENRMNALISFGNSLLYTTVLSEIYKTHLDPR
                     IGYLHATNFRRFTLNLDVAEIFKPILVDRVIFYLLNKKVIGPKNFDERLGGIYLDESG
                     RRAFVERFEERLKTTINHSKIGKQVSYRRLIRLELYKLEKHLMGEENYTPFGAEW"
     gene            complement(1587..2084)
                     /gene="cas4"
                     /locus_tag="PKN31_06980"
     CDS             complement(1587..2084)
                     /gene="cas4"
                     /locus_tag="PKN31_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOA99703.1"
                     /translation="MVEDISGTLVQSYSICKRQSWLMAHQVTPDQDYYYLEMGRFLDT
                     ESYDRDKKKIHFENVVLDLIRTRQGNLVVGEIKKSSRAEESARLQLLFYLYRLRERGV
                     YSKGVLLFPKERKKIEVDLTPELEKEIKSILEDIREVIALPSAPPPQKIKYCTHCAYR
                     EFCWS"
     gene            complement(2078..4435)
                     /gene="cas3"
                     /locus_tag="PKN31_06985"
     CDS             complement(2078..4435)
                     /gene="cas3"
                     /locus_tag="PKN31_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOA99704.1"
                     /translation="MARAYSFSNLYSHPDKYLEEHLNQVAQIAVATWEATPVTTLGEF
                     SKNDLTRLIKICGLAHDLGKSTNSFQEYLFSENKEKANETRHSLLSAIATFFLVQDEF
                     REATHLSEDQKKFLSFVGFLAVKRHHGNLQDVLAEAFFDAKEKEALLKQIGDIDADRL
                     SVLVENLKSSGLPSNITRDSWSGWVEAFSLETIKLRRELRNLSREKNLNWYFLTNLVF
                     SLLIDADKSEATGGEVPRKAIDISPSIVSDYKSTLKKEETCLNQLREEAYQKVVNQEI
                     DLTTRIFSLNLPTGLGKTFTSFAFVLRLREKIKEEKGYTPRIIYSLPFLSIIEQNAEQ
                     IEKTLINSGLAVDSSVILKHHHLSDIYYQEGTKEFEPDQAKILIEGWNAEIVVTTFVQ
                     FFHTLISNRNSTLRKFHRLAGSIVILDEVQSIPFKYWLLLKEVFRQLTSSFNSYIILV
                     TATQPLILEKEEVFPLVDPQKYFPRVNRVTIQPSLEQDITIEELVGDLVIEDDKSYLF
                     VMNTISAALLLYQLMKEKVGDKIAYLSSHVVPWERLKRIKEIQNKKFRLAVTTQLVEA
                     GVDIDFDVIYRDIAPLDSLIQSAGRCNRNWSKEEGLINVIRLRDDKRTYSSYIYDSLL
                     LRETEKVLLKSNFLQEQDFFGMVENYFHLLQNKKSSDESRELLEAIYRLRYSSTDQRA
                     GIADFKLIEEDYPKLEVFLEINQEAQEIWNAFLEIKEIKDRWERRAAFDKIKSGFYQF
                     VVSVPLSVENLPPEVSGFRYVNNDSLCDFYDSETGFKLKGGPAIW"
     gene            complement(4410..>4697)
                     /locus_tag="PKN31_06990"
     CDS             complement(4410..>4697)
                     /locus_tag="PKN31_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HOA99705.1"
                     /translation="ELLADFQYQKVVEVEESKEGETDLVTVIPLPYLEGLRIIFEGGK
                     KYFKERIPIRMNEERIVEEYQEVIFESGGKTIQAETKRHWKSESGESLLFF"
BASE COUNT         1322 a          987 c          841 g         1547 t
ORIGIN      
        1 caatccctta taggtaggct aaaaacggag gtatataaaa atgactactg atgctactga
       61 aatctgtttc aatcccttat aggtaggcta aaaacccaat aattgatacc atttacgttt
      121 tcctatactc tcgtttcaat cccttatagg taggctaaaa acccgttcga ggggcttaaa
      181 ataaggattc cttattttct atttagtttt caaggtgcgt gcctgaaatt attaaaaact
      241 attattatca ttttgtcaaa cgaaaataat agatttttcc ctttctgtcg atccccgggg
      301 gtttttgcgc taccggaggt cgacagaaaa ttaaatgatt acctggtgcc cgcctttgct
      361 aaggcctatt atttctcgtt tggtataacg agtactacgt aaaacataga agattactga
      421 gtcttcttcg agctctatcg ttttggtgat ttctactttg agtttttcat atttggcgtc
      481 ggttatttct ccctccaaaa ccgaattttg aacccaattg agatattttc gggcaatttt
      541 caaaacttta gccactcttt tctggtttac gtcatatacc ataataacaa acattcacca
      601 ttcagctcca aaaggagtgt aattctcttc ccccatcaag tgtttttcca gcttatataa
      661 ttccagccgg atgagtctcc ggtaagagac ctgttttcct attttactgt ggttgatagt
      721 agtttttaat ctttcttcga atctttctac gaaagctcgt cggcccgatt catctaaata
      781 tattccaccc aatcgttcat caaagttttt tgggcctatc actttcttat ttaataagta
      841 aaaaataacc cggtcaacca atatgggttt gaagatttca gctacatcta agttcaaggt
      901 aaaccttcga aagttggtag cgtgcagata cccaatacgg gggtccagat gagttttata
      961 aatttcggat aacacagtag tgtaaagcag ggaattgcca aaactgataa gagcgttcat
     1021 tctgttttct ggtggtcttt tggttcggcc ctccattgta aaatcttcct tttggactat
     1081 aaggtcgaaa gaagaataat atatctgccg ggcattaccc tcaatagcca tcaacctctc
     1141 gattgtattt tgttcctctg ccgattgaga aaaggaattt atcttttcta ttaatggtga
     1201 taactcaatt cctcggttcc cataatattt caaaactttt tccatattct gaagagcacc
     1261 tcgaacaaag ctcttagcta aaattaatcg tttttcttca tccagataat gttctgcctg
     1321 ttttaagata agatagccag aattgtagtg ctctcgaggg taaaagctcc ccgaataata
     1381 accgtaatag ttgtaaaaat ggagcaatat ttccttttga gaaagatatt caagtatttt
     1441 tttgttaaga gaaacttccc cgaagatgtg aagttctcga gtattttcga taggaatata
     1501 tttgcgcttc ccttcactat cttcaaagta gatagtgttg tgtttccgct cgagtttgcc
     1561 actggaaaaa acataaatgc ttttactcat gaccagcaaa actccctata cgcacaatga
     1621 gtgcagtatt ttattttttg aggaggaggg gcagaaggca gggcgattac ctcccttata
     1681 tcttccagga tgctttttat ttctttttct aattcgggag ttaggtctac ttctattttt
     1741 ttcctctctt tggggaaaag caaaactcct ttcgaataga cccctctctc tcgtaatcgg
     1801 taaagataaa acaacagctg tagcctggca ctttcttccg ctcgagaact cttttttatt
     1861 tcccctacca ctaaatttcc ctgtctggtt cgaattaaat ctaaaaccac gttttcaaag
     1921 tgaatttttt tcttgtctcg gtcatagctc tccgtgtcta aaaatctgcc catttccaga
     1981 taataataat cctggtcagg ggtaacttga tgagccataa gccaggattg ccttttgcaa
     2041 atggagtagc tttgaaccag agttcctgat atgtcttcta ccatatggct ggccctcctt
     2101 tcaatttgaa acccgtttct gaatcataga agtcacagag gctgtcattg ttaacatagc
     2161 gaaaaccgga aacttcaggg ggaagatttt ccacacttag tggtaccgaa accacgaatt
     2221 ggtaaaaacc actctttatt ttatcgaatg ccgcacgcct ctcccaccgg tctttaatct
     2281 ctttgatttc taaaaaagca ttccaaattt cctgtgcttc ttgatttatt tccaaaaaaa
     2341 cttccagttt aggataatct tcttcaatca acttaaaatc tgctatccct gctcgttgat
     2401 cggtgctgct gtatcttaat cgatagatag cctctaacag ctctctggat tcatcgctcg
     2461 atttcttatt ttgcaacagg tgaaaataat tctccaccat cccaaaaaag tcttgttcct
     2521 gaagaaagtt acttttcaac agaacttttt ccgtttctcg aagaagcaag gagtcgtaaa
     2581 tataagaaga gtaggtgcgc ttatcatctc tcaggcgtat tacgtttatt aatccttctt
     2641 ccttgctcca gttccgatta catctcccgg cagactggat gagagaatca agaggagcaa
     2701 tgtctcgata aattacatca aaatctatat ccactcctgc ctctactaat tgagtggtaa
     2761 cagccaggcg aaacttctta ttttgtattt cctttattcg tttcagacgt tcccagggga
     2821 caacatgcga ggagaggtag gctattttgt ctccaacttt ttctttcatc aattgataaa
     2881 gcaaaagagc agcgcttata gtattcataa caaagagata gcttttgtca tcttctatta
     2941 ctaaatcacc gactaactct tctatagtga tatcctgctc taaacttggc tgaatagtaa
     3001 ctcgatttac tcggggaaaa tatttttgag ggtcaaccaa gggaaagact tcttcttttt
     3061 ctaatattaa aggctgtgta gcggttacca aaattatgta ggagttaaac gatgaggtca
     3121 gttgccgaaa aacctctttt agaagaagcc agtacttaaa aggtatagat tgaacttcat
     3181 ctaaaattac aatcgagcct gctaatcggt gaaacttgcg tagggtggag tttcgattag
     3241 aaattaaagt atgaaaaaac tgaacaaaag tggtaacgac aatctctgca ttccatcctt
     3301 ctatcaaaat tttcgcctgg tcaggttcaa actcttttgt accttcctga tagtaaatat
     3361 ctgagagatg gtgatgtttt aaaatgacag agctatctac tgcaagacca ctatttatta
     3421 aagttttctc tatctgttcg gcattttgtt caataatact taaaaaagga agagagtata
     3481 taatacgagg agtataaccc ttctcttctt ttattttttc tcgaagcctc aagacaaaag
     3541 cgaaggaggt aaaggttttc cctaatcccg tgggaagatt cagactaaaa attctggtgg
     3601 ttaagtctat ttcttggtta accaccttct ggtaagcttc ttcccttaac tgatttaagc
     3661 aggtttcttc tttctttaaa gtggatttat agtcacttac tatggaagga gaaatatcta
     3721 ttgcttttct gggaacctct ccccccgttg cttcgctttt gtctgcatca atcaaaagag
     3781 aaaaaactaa attggtcaag aaataccagt tgagattttt ctccctactc aggttgcgca
     3841 attctcttct caacttgatt gtttctaagg aaaaagcttc cacccatcca gaccaggaat
     3901 ccctggtaat atttgatgga aggccagagc ttttgagatt ttctaccagc acagaaagcc
     3961 ggtctgcgtc tatatctcct atttgcttta aaagagcttc tttttctttg gcatcaaaga
     4021 aagcctcagc cagaacatct tgcagattcc cgtggtgtct cttgactgcc aggaagccca
     4081 caaaagatag aaacttcttt tggtcttctg atagatgggt tgcttctcgg aattcatcct
     4141 gaaccagaaa aaaggtagca atcgccgaga gaagggagtg acgagtttca ttggcttttt
     4201 ccttgttttc tgaaaaaaga tactcctgaa aagaattagt agatttgccc aggtcgtgag
     4261 ctaacccaca gattttgatt aatctggtca aatcgttttt agaaaattct cccagggtag
     4321 ttaccggagt agcctcccag gtggccactg ctatttgagc cacctggttt aaatgctctt
     4381 ccaagtattt atcgggatga gaatagagat tagaaaaaga gtaggctctc gccactttcg
     4441 cttttccagt gtcgttttgt ttcagcctga atggtcttac ctcctgattc aaagataacc
     4501 tcttgatatt cttctactat cctctcctca ttcatccgga tgggaattct ctctttaaaa
     4561 tatttcttcc ctccttcaaa gattatccgc aaaccctcca ggtaaggaag gggaatgacg
     4621 gtaaccaaat ccgtttcccc ttccttactc tcttccacct ccactacctt ttgatattgg
     4681 aaatcagcca ggagctc
//
LOCUS       DAOPSS010000124         4672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_14859_length_4672_cov_7.896036, whole genome shotgun sequence.
ACCESSION   DAOPSS010000124 DAOPSS010000000
VERSION     DAOPSS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14859_length_4672_cov_7.896036"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKN31_06995"
     CDS             <1..404
                     /locus_tag="PKN31_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOA99706.1"
                     /translation="NMAIVLDEYGGTAGLVTIEDLLEELVGEIRDEHDRETAPYRKIG
                     DDEYLVDASLPIESLNELLGLSLPESEEFESVGGLIMELLGKVPEEGEMVEVDDYQLK
                     VERMKGKRILTLRLWVKKKGEEENGEANYSS"
     gene            379..1905
                     /gene="xylB"
                     /locus_tag="PKN31_07000"
     CDS             379..1905
                     /gene="xylB"
                     /locus_tag="PKN31_07000"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOA99707.1"
                     /translation="MGKLIIAHDLGTTGNKATLFSEEGEKIASEFFGYDTYFPDALSV
                     EQNAEDYWEAVVCSTRRLLEKGKVKKEDIGVISFSGQMMGALPVDKNGQPLYRILIWA
                     DRRGVEEVEWIKERIGEDRIYQLTGHRLSANYSLAKILWFRNHLPEIYNKAHKFLLAK
                     DFVVFRLTGQWATDFSDASGTNLFDLARETWSEEILEKVNIEEDKLPPLYPSTMVVGE
                     VTKEAGEECGLLPGTPVVIGGGDGPCAACGAGVVGEGEAYLYLGSSSWIAFASSQPFY
                     DPEKRTFTFHHLEKGLFMPTGTMQSAGGSYQWCRDALCEREKILAQELGVSAYQLMDL
                     EASKVPPGCDGLLFLPYLLGERAPWWNPHARGAFIGLTPSHNKPYLIRSVLEGVSLNL
                     RIILDAFREQGLCPDALRLIGGGAKGDFWSNLLAQVLGVEILRPRYLEEATSLGAAIA
                     GGVGVGIFKGLEVAKELIEIEDHFSPRAETVETYNRLYPIFKKAYLSLLPVFDDLSSF
                     "
     gene            complement(1902..2714)
                     /locus_tag="PKN31_07005"
     CDS             complement(1902..2714)
                     /locus_tag="PKN31_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HOA99708.1"
                     /translation="MPYIAFLTDWGHKSYYVGVTKAVIKKINPQVEIIDLTHEVEVFN
                     VREAMYVLFRAFLDFPSESIFLAVVDYGVGTERKALALQLEKGYTFVGPDNGLFTLIA
                     ENEKVVRIIELNHPQYFYHPLPSSTFHGRDIFAPCAAHLSRGIPIDKLGSPVDLKDLT
                     FLPYQKPEIKNHSLQGEIAYTDRFGNLETNLPRDYLQEIEIKEGEKLIIQIENKKFIA
                     TYQLPYAAVSQGELLVHPDSSGYLEIAAYQGSAQVITKARGGEKVLLQKFIP"
     assembly_gap    2754..2853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3014..3925)
                     /gene="ilvE"
                     /locus_tag="PKN31_07010"
     CDS             complement(3014..3925)
                     /gene="ilvE"
                     /locus_tag="PKN31_07010"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOA99709.1"
                     /translation="MQELEKIWINGDLVPWKEAKVHVLTHALHYGTAIFEGIRCYKTN
                     SGPAVFRLQDHLKRLYDSAHIVRMAIPYSPEELEKGIIDTIKANKLEECYIRPIVFRG
                     YGEMGIDPSSCGVDCVIAVWRWGAYMGEKGIKEGIRAKISSYTRTYVNSGSPRAKTSA
                     NYLNSSLAKVEAKDLGYDEAILLDINGFVSEGSGENIFYVKDGVIFTPHPYSILLGIT
                     RDTVIKIARKLGMEVKETLCTRDDLYLADEAFFTGTAAEITPIIEVDGRTIGTGKRGE
                     ITRLLQEVFFRAVKGEEPDYESWLTKL"
     gene            complement(4005..>4672)
                     /locus_tag="PKN31_07015"
     CDS             complement(4005..>4672)
                     /locus_tag="PKN31_07015"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOA99710.1"
                     /translation="FKTTLEKEHSKKRKRLKTNNRGKTKKYPSNRLILSTLQRLLQLT
                     ITMLYNISVKANINAANTARLGILVSLGIVLHILEGMLPSFFLPGAKLGLANVVTLTI
                     LWRGNWIEAIEVAILRIVLGNILSGNLGVGFFLSLGGGLASLGGMFLSQKKGFSLLVV
                     SIVGALSHNLGQLIIAYFYMKSQSIFYYLPFLVLFSLPTGLLIGYLGQFLVRWEEKIF
                     SPS"
BASE COUNT         1257 a          889 c         1018 g         1408 t
ORIGIN      
        1 ttaacatggc tattgttctg gatgaatatg gaggtacagc aggattagtt accattgaag
       61 atcttttaga agaattggtg ggagaaataa gagacgagca tgaccgggaa actgctcctt
      121 atcggaagat cggggatgat gagtatctgg tagatgcttc cctgcccatt gagagcctca
      181 atgaactttt gggtttatcg cttcctgaga gcgaagagtt tgagtcggta ggaggcttga
      241 ttatggaact tttggggaaa gttcccgagg aaggagaaat ggtagaagtg gatgattatc
      301 agctaaaagt ggaaagaatg aaagggaaaa ggatcctcac cctgcgtctg tgggtgaaaa
      361 agaaaggaga ggaagaaaat ggggaagcta attatagctc atgatttagg aactaccggt
      421 aacaaagcta ctctttttag cgaagaagga gaaaaaatag ccagtgagtt ttttgggtat
      481 gatacttatt ttcctgatgc tctttcagta gaacagaatg cagaagacta ttgggaggcg
      541 gtggtttgtt ctactcgccg gttactggaa aaaggaaaag taaaaaagga agatattgga
      601 gttatttctt tttctggaca aatgatggga gcattaccgg tggataaaaa tggtcaacct
      661 ctctatcgta ttcttatctg ggcagatagg agaggagtag aagaagtaga atggataaaa
      721 gaaaggatag gggaggaccg gatttatcaa ttgaccggcc atcgtctcag tgctaactat
      781 tccctggcaa aaattctctg gtttcgtaat cacttaccag aaatttataa taaggcccat
      841 aagtttcttt tagctaaaga ttttgtggtt tttaggttga ccggtcagtg ggcaaccgac
      901 ttttccgatg cttcaggaac caatcttttt gatctggctc gggaaacctg gtcagaggag
      961 atactggaga aagtaaacat agaagaggat aagcttcctc ctctctatcc ttctacaatg
     1021 gtggtgggag aagttactaa ggaagcgggc gaagaatgcg gacttttacc tggaactccg
     1081 gtggtcattg ggggaggaga cggtccttgc gctgcttgcg gggcgggggt agtgggagaa
     1141 ggagaagcct atctttatct tggttcttct tcctggattg cttttgcctc ttctcaacct
     1201 ttttatgacc cggagaaaag gactttcacc tttcatcatt tagaaaaagg tctttttatg
     1261 cccacaggca ctatgcaatc ggctggtgga tcatatcaat ggtgtcgaga tgcactttgt
     1321 gagagggaaa aaattctggc tcaagagctg ggagtaagtg cttatcaatt gatggatcta
     1381 gaggccagca aggttcctcc tgggtgcgat ggtttacttt ttctacctta tcttttaggt
     1441 gagagagctc catggtggaa tcctcatgct cgaggagcat tcataggctt aaccccctct
     1501 cataataaac cctaccttat tcgatcggtt ctcgaaggcg tcagtcttaa tcttcggata
     1561 atacttgatg cttttcggga acagggactc tgtcctgacg ctttgcgcct catcggcgga
     1621 ggagcaaagg gagatttttg gtctaacctt ttggctcaag ttctgggagt agaaattttg
     1681 cgtcctcgat atctggagga ggctacttcc ttaggggcgg ctattgctgg gggagtgggg
     1741 gtggggatat ttaagggatt agaagtggca aaggaattga tagagataga agaccatttt
     1801 tcgcctcggg cggaaacagt ggagacctac aatcgtttgt accccatatt taaaaaagct
     1861 tatctttctc ttcttccagt gtttgatgat ttgagttcct tttaaggaat gaatttttgg
     1921 agaagaactt tttcccctcc ccttgctttt gttattactt gagcactacc ctggtaagca
     1981 gcaatctcta aataccctga gctatcgggg tggacgagaa gctctccctg ggaaactgca
     2041 gcgtagggaa gttgataagt agctataaat tttttattct ctatctggat aatcaacttt
     2101 tctccctctt ttatttctat ctcctggaga taatctctgg gaagattggt ttctagattg
     2161 ccaaagcggt cggtataagc tatctcgcct tgcaagctgt gatttttaat ctcgggtttc
     2221 tggtagggta aaaaagtcaa atctttgaga tccacgggag agcccagctt atcaatcggt
     2281 attcctctgg ataagtgagc ggcacagggg gcaaatatgt ctcttccatg gaaagtagag
     2341 gagggaagag ggtggtaaaa atattgagga tggtttaact ctattattct taccaccttt
     2401 tcattttcag ctattaaggt gaaaagacca ttatcagggc ccacaaaagt atagcctttt
     2461 tccagttgca gagctaaagc ttttctttct gttcctactc catagtctac cacggctaaa
     2521 aatatgcttt ccgatggaaa atcgagaaac gcccggaaga gcacatacat tgcttctcga
     2581 acgttaaaaa cttccacttc atgagtcagg tcaatgattt ccacctgagg atttatcttt
     2641 ttaattaccg ctttggttac tcctacataa taacttttat gtccccagtc agttaaaaaa
     2701 gctatgtaag gcatgattta ccgataaaaa taattttatt ctttttcagg ctannnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttttta gctttaagat ggagggttcc
     2881 ttcgttcatt atttttccaa aaaactgatt aaagtctttg agtggtgggg aaagcctttc
     2941 ttttatatca cgcatccctt cttggttctc tttaaaggat tcttgtggac tggtaattaa
     3001 ccccttcact tcactataac ttggttaacc agctttcata atctggttct tctcctttca
     3061 ccgcccggaa aaagacttct tgcaggagac gagttatctc tcctcgtttt cctgtgccaa
     3121 ttgttcttcc atctacttct atgatgggag taatttccgc tgctgttcca gtaaagaaag
     3181 cttcatctgc gagataaagg tcatctcgag tgcagagagt ttctttcact tccatgccca
     3241 gtttacgggc gatttttatt accgtatccc gggtaatacc taaaagaatg gagtaagggt
     3301 gaggggtaaa aattactcca tctttcacat aaaatatgtt ctctccgctc ccttcagaaa
     3361 caaagccatt gatatctaat aaaattgctt cgtcatagcc taaatctttt gcttctactt
     3421 tggctaaact tgagtttaga taattggcac tggttttagc tcggggagaa ccagaattaa
     3481 cgtaagtcct ggtgtaagag gatattttgg ctcgaatgcc ttctttaatt cccttttctc
     3541 ccatgtaagc tccccagcgc catactgcta ttacgcaatc gacaccacaa gatgaagggt
     3601 ctatccccat ttctccatac ccccggaaga caatcggtct tatataacat tcctctagtt
     3661 tgttagcttt aatggtatct attatccctt tttccagttc ttccggagaa taaggaatag
     3721 ccatacgcac gatatgggct gagtcataaa gccttttcag gtggtcctgt agacggaata
     3781 ctgcaggacc actattggtt ttgtaacagc ggattccttc aaaaatggct gttccgtagt
     3841 gcagagcatg ggttaagacg tgaactttag cttccttcca gggtaccaaa tcaccgttta
     3901 tccagatttt ttctaattct tgcatttcaa cgctccttat cttatattgc attccttata
     3961 ttctattaga tgaaggcaaa tttgccaatg caggggaaag aaaattagga cggagaaaat
     4021 attttttctt cccaccgtac taaaaactgc cccaaatagc ctatcaataa accagtggga
     4081 agagaaaaaa ggacgaggaa aggaaggtag taaaaaatac tctggctttt catataaaaa
     4141 taggctatta ttaactgtcc caaattatga gaaagtgctc ccacaatgct gactaccaaa
     4201 agagaaaatc cctttttttg agagagaaac atgcctccta agctggctaa tcctcctcct
     4261 aagcttaaga aaaagcctac tcctaagttg ccacttaaga tattgcccag cacgatgcgc
     4321 aatatagcta cctctatggc ttctatccaa tttcctctcc ataaaattgt caaagttacc
     4381 acattggcca aacctaactt tgctcccggc aggaaaaaag aaggaagcat accctctaat
     4441 atgtgtaaca ctatgcccag tgaaactaaa atccctagac gagctgtgtt agccgcattt
     4501 atgtttgctt tcacggagat attatatagc atagttatgg ttaactgcaa aagccgttgt
     4561 aatgtcgata gtatcaatct attggagggg tatttttttg tttttcctct attattagtt
     4621 tttaatcttt ttcttttttt tgagtgctct ttctccagtg tggttttaaa tt
//
LOCUS       DAOPSS010000125         4590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_15203_length_4590_cov_8.897905, whole genome shotgun sequence.
ACCESSION   DAOPSS010000125 DAOPSS010000000
VERSION     DAOPSS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15203_length_4590_cov_8.897905"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PKN31_07020"
     CDS             <1..1138
                     /locus_tag="PKN31_07020"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOA99711.1"
                     /translation="PFFIFFLLAFLLELMPVYLQGGVALSISFTLVYAALLLFSPYFA
                     ASVAFLSAFFATIKSPYYASLFNASQYALSAFLSGQVFQMLGGYEFSWQSLPFYGVAA
                     VSILVFFVSNTFLVGGVISLNQGAPLRLFWREKNIREILFQYFALFPFSLLLYLVYTY
                     IGYWGIILFFFPLMVARYSFKLFADTKKNHLQLLQALIAALDARDSYTAGHSSRVAEL
                     SLQIAEHLHLPPQKREALEYAAVLHDVGKLGIQDSILTKQGELSEDEHSLVCEHPEIG
                     YKILGEVDFLQEAAQIVLAHHERMDGSGYPEGKKGEEIPIEARIVGAADVFDALTSDR
                     PYRRAYSAEEALEIMENEEKGSFDPEVLQILRKIVEEKREKNVS"
     gene            1128..1664
                     /locus_tag="PKN31_07025"
     CDS             1128..1664
                     /locus_tag="PKN31_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HOA99712.1"
                     /translation="MLVDFIVVGLIWGFLRGGKLRNLSQVDFPFLWVIILGFGIEFLA
                     LAGLQPFVVFNLAGRGLVLLGALLNIRLYGMKSMSLGASLNFLVIALNGGKMPVSLEV
                     TSWLGLQKYVELLENNYYPDYTIISSSTLLPFLGDVLPYFSFLFRRFFVASLGDYFLG
                     IGVMLFLVYHLRKEEVKA"
     gene            1661..2539
                     /gene="galU"
                     /locus_tag="PKN31_07030"
     CDS             1661..2539
                     /gene="galU"
                     /locus_tag="PKN31_07030"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOA99713.1"
                     /translation="MKKVKKAVIPVAGLGTRLLPATKTQPKEMLPIVDKPAVQYVVEE
                     AITSGIESILFVTGRGKRAIEDYFDYSVELEQELENRGKEELLDEIKKVGNLVPVYYV
                     RQKSPRGLGDAVYQAREFVGEEPFALILADDIFVTPSPLLGEIMELYRQKPAIYLTVT
                     PVPPQDVSSWGVIKGEKVGEALYRVEDLVEKPEKDKAPSSLAIVGRYVLTPSIFEAIE
                     CTPPGRGGEIQLTDAIRALLGREEVYALELRGKHFGVGDKVGYLKANIAFALEREDIG
                     EEIKTFLREILQEDDR"
     gene            2552..4096
                     /locus_tag="PKN31_07035"
     CDS             2552..4096
                     /locus_tag="PKN31_07035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA99714.1"
                     /translation="MQLTFLGATREVTGSCYLLKTRDKNFLVDCGLIQGKGEERNYQP
                     FSFSPGDIDFILLTHAHLDHSGRIPLLCRHGFRGKIYATPPTIELTHLLWLDTAKIMR
                     EEAERINRKNVRQGKPPIQPLFGEEEVEEAMNLFEPVSYDELVSEGEVEFVFRDSAHI
                     LGGAALEIWGEGSKVVFSGDLGQFYSVMEGSPPVIERADFVVIESTYGNRRHRSLEDT
                     RREFSEAIEGALSKGGKVLIPSFVVDRAQRIIYELFLLQERLDGKYPVFFDSPMGVKV
                     TDIYRQYRNLLAGEIQKLNLEKIDPFSLPGLHYVTTPAESKAINNINQAIVIAGSGMC
                     TGGRIIHHLKHNLYKENTALIFVGFQAQGTLGRALVEGAKRVRIMGEEIVVRAKIYTI
                     NGFSVHADQEDLFRWVEYFQGNPTFLVTHGEEAIAESFAQFLGERGKEALVPHLGDTM
                     DLAKKKVFSAPSLPPEENILEQLESKVSSLESIYPALGEEEKILLRSALILLEEVERR
                     KEESLI"
     gene            complement(4093..>4590)
                     /locus_tag="PKN31_07040"
     CDS             complement(4093..>4590)
                     /locus_tag="PKN31_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99715.1"
                     /translation="RGTKTQLSPDGIIIVEGLHALNPQLGENIPGGEKFKIYVSAITQ
                     INVDDHNRISTTDSRLIRRLVRDSQFRGSRGEGVFADWPRVREGEEKYIFPYQESADI
                     MFNSSLIYELSILRQYAEPLLRAITPESPHYLEAYRLYAFLNHFMPLNPSFIPSNSIL
                     REFIG"
BASE COUNT         1191 a          836 c         1132 g         1431 t
ORIGIN      
        1 tccttttttc attttttttc ttctggcttt tcttctggag ctgatgcccg tttacctaca
       61 gggaggggta gcgctttcca ttagcttcac tttggtttat gctgctttac tcttgttttc
      121 tccctatttc gcggctagtg tggctttttt atctgcgttt tttgcgacca tcaaatcccc
      181 ctattatgct tctcttttca acgcttctca gtatgctctt tctgcttttt tatccggtca
      241 agtgtttcaa atgctgggag gatatgagtt ttcctggcag agcttacctt tttatggggt
      301 agcggcagta tccatccttg ttttttttgt ttccaacact tttttggtgg gaggagtgat
      361 ttccctaaac cagggggcac ctctccgtct cttttggagg gagaagaata tacgagaaat
      421 tcttttccag tactttgctc tttttccttt ttctttactt ttgtatctgg tgtatactta
      481 tataggttac tgggggataa ttcttttttt cttccccctt atggtagctc gttattcttt
      541 taaacttttt gccgatacca aaaaaaacca tctgcagctc ctgcaggcct taattgcggc
      601 actggatgcc agagactcct ataccgccgg tcattcttcg cgagtggctg agctttctct
      661 ccagattgcc gagcatcttc atctccctcc ccaaaaaaga gaggctttgg aatacgccgc
      721 tgttttgcac gatgtgggta agcttggtat tcaggattct attttgacta aacaaggaga
      781 attatctgaa gatgaacatt cattggtgtg tgaacatccc gagattggtt ataagatttt
      841 aggagaagta gatttcctgc aagaagcagc acagattgta ttagctcacc atgagcgaat
      901 ggatggctct ggttaccctg aagggaaaaa gggggaggag atacctatag aggctcgcat
      961 tgtgggagca gctgatgttt ttgatgctct tacctcagat cgcccttacc gtcgggctta
     1021 ttcggcggag gaagctctcg aaattatgga aaacgaggaa aaagggagct ttgatccgga
     1081 agttctccag attttaagaa aaatagtgga ggaaaaaaga gaaaagaatg ttagttgatt
     1141 ttattgtggt gggattgata tggggatttt tgagaggggg aaagttgcgt aatctttccc
     1201 aggttgattt cccttttctc tgggttatca tcctgggttt tggcatagaa ttcctggctc
     1261 tggcgggact gcaacctttt gtagttttta atctggcagg gagaggactc gtccttttag
     1321 gagctttatt gaacattcgt ctctatggta tgaagtcaat gtctctggga gcttctctaa
     1381 attttttggt tatagctctt aatgggggaa aaatgcccgt gtctttagaa gtgacttcct
     1441 ggctgggtct tcaaaaatat gtagagcttt tagagaataa ttattatcct gactatacca
     1501 ttatttcttc ttccactctt ttaccttttt tgggagatgt gttaccttat ttttcttttc
     1561 tttttcgccg tttttttgtg gccagcctgg gagattattt tttggggata ggagtaatgc
     1621 tttttctggt ctaccatctg agaaaggagg aggtaaaagc atgaaaaaag taaaaaaagc
     1681 ggtcattcct gtcgcgggtt tggggactcg ccttttgcct gccactaaaa ctcaacctaa
     1741 agaaatgtta cccattgtgg ataaaccagc cgttcaatat gtggtagaag aagctataac
     1801 ttcaggcata gaaagcattt tatttgttac cggaagggga aagagagcta ttgaggatta
     1861 cttcgattat tcggtagagc tggagcagga attggagaat cgggggaaag aggagttatt
     1921 ggatgaaata aagaaggtgg gaaacctagt tcctgtttat tatgtacggc agaaatcccc
     1981 tcgaggtttg ggggatgcgg tttatcaagc tcgagaattt gtgggagaag aaccttttgc
     2041 tcttatttta gctgatgata tttttgttac cccttctcct cttctgggag aaattatgga
     2101 actttatcgt caaaaaccgg ctatctacct gacagttact ccagtcccgc cccaggatgt
     2161 ttcttcctgg ggggtgataa aaggagaaaa agtgggggaa gctctttatc gggtggagga
     2221 cttagtagaa aaaccagaaa aagacaaggc tccctcctct ttagctattg tggggcgcta
     2281 tgttttaacc ccttctattt ttgaggctat cgaatgtact ccgccaggaa gaggaggaga
     2341 aattcagctc accgatgcta taagagctct tctgggaaga gaggaggttt atgctctgga
     2401 actccggggt aagcattttg gcgtgggaga caaggttggt tatcttaaag ctaacattgc
     2461 ttttgctctg gagcgagaag atataggaga ggaaattaaa acttttttgc gagaaatttt
     2521 gcaggaagac gacagataag gaggtagtgg tttgcaactt acttttttag gagcgactcg
     2581 agaggttact ggttcttgtt atcttttgaa aaccagggat aaaaattttc ttgtggattg
     2641 cggtctgata caagggaaag gggaggagag aaattatcag cctttctctt tttctcccgg
     2701 agatattgat tttatccttc tcactcacgc ccatctggat cactctggta gaataccttt
     2761 actttgtcgt catgggtttc gagggaaaat ttatgctacc cctcccacca ttgagctcac
     2821 ccatcttttg tggctggata cggcaaagat tatgagggaa gaagcagaaa gaataaaccg
     2881 aaaaaatgtt cggcaaggca agccccccat tcaacctctt tttggagagg aagaagtaga
     2941 ggaggctatg aacctttttg agccggtgag ctatgatgaa ttagtcagtg aaggagaggt
     3001 ggaatttgtt tttcgcgatt ctgcccatat tttaggggga gcggcgcttg agatatgggg
     3061 agaaggaagt aaagtagttt tttccggaga cttagggcaa ttctatagtg tgatggaagg
     3121 ttctcctccg gtcatagaaa gagcggattt tgtagtgata gaatctacct atggtaaccg
     3181 tcgtcatcga agcttagaag atactcgccg ggagttttcc gaggctatag agggagcttt
     3241 atctaaagga ggaaaagttt taattccttc ttttgtggtg gatcgggccc agcggataat
     3301 ttatgagctt tttcttttac aagaaagatt agatggaaaa tacccggtgt tttttgatag
     3361 ccctatgggg gtcaaagtca ctgatattta tcgacaatac cggaaccttc ttgccggaga
     3421 gattcagaag cttaacttag agaagataga tcctttttct ctaccgggct tgcattatgt
     3481 taccactcct gcagaatcaa aagctatcaa caacataaat caagctatag taattgccgg
     3541 aagtggaatg tgcacaggag gaagaattat ccatcactta aaacataatc tctataagga
     3601 aaataccgct cttatctttg tgggatttca ggctcaaggg actttgggga gagctctcgt
     3661 agaaggagcc aaaagagttc ggattatggg agaagagata gtagtccgag ctaaaatata
     3721 tactattaac ggtttttctg tgcatgctga tcaggaagac ctgttcaggt gggtggaata
     3781 tttccagggg aaccccactt ttctggtaac tcacggtgag gaagccattg ctgagtcttt
     3841 tgctcagttt ttaggagagc gaggaaaaga ggcgctggtt ccgcatctcg gggataccat
     3901 ggatttggcg aaaaagaaag tattcagcgc tccttctctt cctccggagg aaaatattct
     3961 ggaacaactg gaaagcaaag tctcttctct tgagagtatc tatcctgctt taggagaaga
     4021 agaaaaaatt cttttgcgct ctgctttgat acttttagaa gaagtggaga ggagaaaaga
     4081 agagagcttg atttagccga tgaactcccg aagaatagag ttagaaggga taaaagaggg
     4141 gttgaggggc ataaagtggt tgaggaaagc atagaggcga taagcttcta aatagtgagg
     4201 gctttcggga gtaatagctc gcaggagagg ttcagcatat tgtcttaaaa tactcagctc
     4261 atatattaaa gaagaattga acataatatc agcactttcc tggtagggga aaatatattt
     4321 ttcctctcct tctcttaccc ggggccagtc ggcaaatact ccttctcctc tgcttcccct
     4381 gaattggcta tctctcacca gacgccgaat caatcgagaa tcggtggtag aaatgcgatt
     4441 gtggtcatcg acgtttattt gagttatagc gcttacataa attttgaatt tttctcctcc
     4501 ggggatattt tctcctaatt ggggattgag agcgtgtaat ccttctacta ttatgatacc
     4561 atcgggagag agctgagttt ttgtccctcg
//
LOCUS       DAOPSS010000126         4537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_15441_length_4537_cov_6.773315, whole genome shotgun sequence.
ACCESSION   DAOPSS010000126 DAOPSS010000000
VERSION     DAOPSS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15441_length_4537_cov_6.773315"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PKN31_07045"
     CDS             complement(<1..737)
                     /locus_tag="PKN31_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOA99716.1"
                     /translation="MRKPSGSGDTRYAYLVGRVRALERGLLTQRTLENLLRSENLEQA
                     LRGVAEIPYWGDVFQNVPPRIYDIDKALVSHYWQTIQEINSHSPGDEIARFFIWPFDF
                     SEMKLILKYRLAGKTLEETYPTTLNLVRVNRFLGGETREYLPDLWIKAINDSLEAYEN
                     YRVIQAVEMVLDRHYLEQVYLLAQEMESRVLKNWLLAFVLLAYFRAVFRARYQERKGE
                     LMRLLYFPQPLFPEEKFIEMLSAPAEKV"
     gene            complement(752..1336)
                     /locus_tag="PKN31_07050"
     CDS             complement(752..1336)
                     /locus_tag="PKN31_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOA99717.1"
                     /translation="MPLEDILRKIEEEAELKKKRILEEARTRASAQLERAQKELKEEA
                     DRLLAERRKEVELEVQRRLAQARLAGKNEVGKVKHQAWEKLQESLVAAFVEKIETEGR
                     EWCKEFLIQYVVSGDEEVWMAPREAEIWGSGFVKEINEEKNLSLRFGGIAEDIERGFL
                     LRRGGMMVDLSFVSVVEEYIRENERRISALLFED"
     gene            complement(1350..1823)
                     /locus_tag="PKN31_07055"
     CDS             complement(1350..1823)
                     /locus_tag="PKN31_07055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOA99718.1"
                     /translation="MIDGLAFALLGSALAIGLAGIGSAMGVGIAGEAGAGVLTEDPNK
                     FGQILLMQALPGTQGIYGLLAGFWVLIKLGLFGGTPVEINVAQGLQILFACLPVAIVG
                     LVSGVYQGRTSAASIGMIARRPEETGRAIILPAMVETYAVLALLATILLLNSIQV"
     gene            complement(1841..3808)
                     /locus_tag="PKN31_07060"
     CDS             complement(1841..3808)
                     /locus_tag="PKN31_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOA99719.1"
                     /translation="MAISAMEKVGVIAHHSLRKGILDQLQDLGVLEITEDEKDLFDEF
                     VSPSVELGELERRLGELSYCLDFIDKYRSEKLGIAQSLFPSPIHMEKEDFLSCSFDYI
                     PLYKTFLDWEEELNNIRSNLNRLNSNLEFMKKIQSVEVPLENLRGTLYTRSFLLEFPP
                     KKKEALEEKLKERNLAWLREEFPGKGGNVLWWVIIHKSEEEAMEEVLAELSLSPVSLP
                     QPFEGTPQEIIEDLEHRIGELQEEQSKLIEKAALQEKFEKEFKIAYDYYLSLWERYQS
                     ETKLLKTKETCYISGWVKKEDLPLVEESISQVGKEWVLYHREPKEGESVPVSLENNSW
                     VNNFSVLTHLYGLPNYTEIDPTPFVAGFFFFFFGMCLGDVIYGLVLALSGFIAAACLD
                     ISDSTKLFLRMLAWGGVGAILVGTFTGSWLGDLFDYFPPSLSFLTTLKNGLMVIDPLN
                     NPLPMLIFSLGVGIVQILVGIMLAVMKEWKRGNYAVAIMDHLSWFIFLVSIVMYIVGI
                     AVSPLLASVALPIIIGGVIFLVATQGRHQKNPIMKFLSGLLSLYDLVSYLGDTLSYSR
                     LFALGLSSTIIAILARTLGSLFGGYPYIGWLIALIVALVFHLFNLLMSGLGAFVHSAR
                     LQYVEFFNKFYEDGGKSFKPFGYKTKYVKIS"
     gene            complement(3798..4121)
                     /locus_tag="PKN31_07065"
     CDS             complement(3798..4121)
                     /locus_tag="PKN31_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99720.1"
                     /translation="MIEEAIQEIRELENLKEREINNAEREAAKIIAGVEDQITEMRKK
                     VMQEVEEKAQALRREMLEKGEKEAKKIEEEFHHQAQKLESIAFSRIKDIVSQLVEEMG
                     EEYGN"
BASE COUNT         1413 a         1111 c          719 g         1294 t
ORIGIN      
        1 actttctctg caggggcact cagcatctct ataaattttt cttcgggaaa aagcggttga
       61 ggaaagtaaa gaagacgcat caactcccct tttctttcct gataacgagc tcggaaaact
      121 gctcggaaat aggctaaaag cacaaaggct agaagccaat ttttgagaac cctgctttcc
      181 atttcttgag ccaagagata aacttgctct aaataatgcc tatctaaaac catttccact
      241 gcctgaataa ctcggtaatt ctcataagct tctaaactat catttatagc tttgatccac
      301 agatcaggga gatattctcg agtttctcct cccaaaaatc tgtttactct taccagattt
      361 aaggtcgtgg ggtaagtctc ttccaaagtc ttacctgcta aacgatactt taaaattaat
      421 ttcatttcac taaaatcaaa aggccagata aaaaaacgag cgatttcatc tcctggagaa
      481 tgactgttta tttcctgtat agtttgccaa tagtgactca ccaaagcttt atcaatatca
      541 taaatacggg gaggaacatt ctgaaaaaca tctccccagt aaggaatctc tgctactccc
      601 cgtagagcct gctctaagtt ttctgaacgg agcaaattct ccagggttct ctgagtcaaa
      661 agacctcgct ctagtgctct tactctacct actaaataag catagcgtgt atctccacta
      721 ccactaggct ttctcaaatc tgtaatcatt gttaatcctc aaaaagcaga gcagaaattc
      781 ttctttcgtt ctctctaatg tactcttcca ccacagaaac aaagctcaaa tctaccatca
      841 tacctcctcg cctcaagaga aagcctcgct ctatgtcctc tgctattccc ccaaagcgta
      901 aagataaatt tttctcttca tttatctcct tcacaaaacc actgccccag atttctgctt
      961 cccgaggagc catccatact tcctcatctc cgctaaccac gtactggata aggaactctt
     1021 tacaccattc cctgccttct gtttctatct tttctacaaa agctgccacc aaactctcct
     1081 gtagtttctc ccaggcctga tgtttaactt tacccacctc atttttccct gctaatcttg
     1141 cctgggcaag cctgcgctgg acttcaagct ctacctcttt tctgcgctct gccaataatc
     1201 gatcagcttc ttcttttaat tccttctgag ccctttccaa ctgagcgctg gctctggtcc
     1261 gggcttcttc taaaattctc ttctttttga gttctgcttc ttcctcaatt ttccgcaaaa
     1321 tatcttctaa aggcataatc tggcccctgt taaacttgaa tagaattcaa aagaagaata
     1381 gtagcaagca aagctaaaac ggcataagtt tctaccatgg caggaagaat aatagccctt
     1441 cctgtttctt ctggtcggcg agcaatcatc ccaatagagg cagctgaagt tcttccctga
     1501 taaactcctg aaacaaggcc tacaatggct acaggaagac aagcaaataa gatttgcaaa
     1561 ccctgagcaa cgtttatttc taccggagtt ccaccaaaaa gacccaattt aattaatacc
     1621 caaaatccag ctaataatcc atagatgccc tgcgttccag gcagcgcctg cataagcaaa
     1681 atttgcccaa acttattagg gtcttcagtt aacacacccg cgcctgcttc tccagcaatg
     1741 cctactccca ttgccgaacc aatccctgct aaccctatag ccaaggcaga acctaaaagt
     1801 gcgaatgcca gtccatcaat catagattac agaacctcct tcagcttatt ttgacgtatt
     1861 tggttttata accaaaaggt ttaaagcttt tacctccatc ttcgtaaaac ttgttgaaga
     1921 actctacata ctgcaaacga gcagaatgaa caaaagcacc taatccactc atcaataaat
     1981 tgaaaaggtg aaacactaaa gccactatca aagcaataag ccatccaata taaggataac
     2041 ctccaaataa agaccctaaa gtacgagcta aaatggcaat gatagtacta gaaagaccta
     2101 aagcaaaaag acgtgaataa gaaagggtat cccccagata agataccaaa tcgtaaagac
     2161 tcaaaaggcc agatagaaac ttcataatgg gatttttttg atgtctgcct tgagtagcta
     2221 ctaaaaaaat aacaccccct attataatag gtaaagccac cgaagctaaa agaggagaga
     2281 cagcaatgcc cactatatac atcactatcg aaaccaaaaa tataaaccag ctcaagtgat
     2341 ccatgatagc tacagcataa ttacctctct tccactcttt catgacagca agcattatac
     2401 ccactaaaat ctgcactata cctactccca aagaaaagat gagcatagga agaggattat
     2461 ttaaaggatc aataaccatt aagccgtttt tcagagtagt taaaaaagat aaagagggag
     2521 gaaaataatc aaaaaggtcc cccagccagg aaccagtaaa agtccccacc aaaatagcgc
     2581 caactcctcc ccaggctagc atacgaagaa aaagcttggt agaatcagaa atatccagac
     2641 aagcggcagc aataaaccca gaaagggcca atactaaacc atatataaca tctcccaaac
     2701 acatgccaaa gaaaaagaag aaaaagcctg ctacaaaagg agtagggtct atttcagtat
     2761 agttaggaag accgtagaga tgagttaaaa ccgaaaaatt gttcacccag gaattatttt
     2821 ccaagctgac gggtacgctc tctccttctt taggctctct atgataaaga acccattctt
     2881 tacccacttg ggagatagat tcttctacca gaggtaaatc ttcttttttt acccatccgc
     2941 taatgtaaca agtctcttta gttttcaaaa gcttggtttc actctgataa cgctcccaca
     3001 ggctcaaata gtaatcataa gctattttaa attctttctc aaatttttct tgtagagctg
     3061 ctttctctat aagctttgat tgttcctctt gaagctcacc aatccggtgt tctaaatctt
     3121 ctattatttc ttgaggagta ccttcaaaag gctgaggaag gctcacaggg gaaagagaaa
     3181 gctcagctaa aacctcttcc atagcttcct cttcgctttt atgtattatc acccaccata
     3241 aaacatttcc cccttttccg ggaaattctt ctctcaacca ggctaaattt ctctccttca
     3301 atttttcttc taatgcttct ttctttttag gaggaaactc cagaagaaag ctgcgagtat
     3361 agagagtgcc tcgcaagttc tccagcggga cctccaccga ttgaattttt ttcataaact
     3421 ccaggttaga atttaaccga tttaagttac tacgaatgtt gttaagctct tcctcccaat
     3481 ccaggaaggt cttataaaga ggaatgtaat caaaactaca gctcaaaaaa tcttcttttt
     3541 ccatatgaat aggagaggga aaaagagact gagcaatccc tagcttttct gagcgatatt
     3601 tatcaataaa atctaagcaa tagctcaact cccccagacg gcgttctaat tctcctagtt
     3661 ctactgaggg agaaacaaac tcatcgaaca agtctttttc gtcttctgtt atttccaaaa
     3721 ctcccaaatc ttgcaactgg tcaagaattc cttttcgtag agagtggtga gcaataacgc
     3781 ccactttctc catcgcacta attgccatat tcttctccca tctcttctac aagctgagaa
     3841 acaatatctt ttatccggga aaaagcaata ctctctaact tttgagcttg atggtggaac
     3901 tcctcttcta tcttcttcgc ttctttttct cccttctcaa gcatctccct tctcaaggct
     3961 tgagctttct cttccacctc ttgcataact ttctttctca tttctgttat ctggtcttct
     4021 actccggcga taattttagc cgcttctcgc tcagcgttgt ttatttctct ttctttcagg
     4081 ttctccagct ctctaatttc ctggatagct tcttcaatca ttttctttac ctccgaacct
     4141 gactaaatag tcacgggata tattagcaaa agagtttatt attggcaaga agaaaatggc
     4201 tctataagaa aaatatccaa gccaagcaca caaaaaaaat tccggataga aggggatata
     4261 tcccctatag cccggatgct aagaaaacac caacccctat tctatcctgt cattgtgaac
     4321 catttaccaa gccatctcta atttttcttt cgggtcacag actgtcacga cggttctaca
     4381 atatttaatg tgaaaccggg tattgaaaac acctgctgac ctctgtatcc taaggattat
     4441 tagctaaaac taaagaaagg aggtcaagag ctgcaagata attatatcgc agtaggatta
     4501 gaattaccac agctccagat tttggaacaa agagagc
//
LOCUS       DAOPSS010000127         4535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_15454_length_4535_cov_7.777232, whole genome shotgun sequence.
ACCESSION   DAOPSS010000127 DAOPSS010000000
VERSION     DAOPSS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15454_length_4535_cov_7.777232"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..763
                     /locus_tag="PKN31_07070"
     CDS             251..763
                     /locus_tag="PKN31_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOA99721.1"
                     /translation="MKEETIAGIFENELAGRVKEYRLIDSQTVVVAEWVRFKCQFGCD
                     GYAQSLTCPPYSPEPSRTRKMLEEYRQAAILWKPEDCETLRKIAADLERTLFLSGYYK
                     ALAIPSGPCELCNPCPLEYPCLHPEYARPSMEACGIDVYATVRQHGFPIEVVRSRSCQ
                     PNYYALILIE"
     gene            1117..2526
                     /locus_tag="PKN31_07075"
     CDS             1117..2526
                     /locus_tag="PKN31_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOA99722.1"
                     /translation="MENRNFRRERDSLGEELIPQNAYWGIHSLRARDNFPISQKVPSS
                     LIKAYFAVKWACARANTEIGLLEENTGNAILKACEEGMQGKFDEEIIVPILQGGAGTS
                     LNMNINEVIANRALEILGHQRGSYQIVNPFDSVNLSQSTNDTFVTAVKVAVLWQLDEL
                     EKEIIFIQEELQQKEREFASIAKVGRTQLQDAVPITLGMEFGAFAEAISRDRRRLEKG
                     KELMKEINLGGTAVGTGLNAHPEYRARVVPYLNEITGLSLTRATNAFENTQNLDVFSE
                     LSGLLRSLAVNLRKIANDLRLLSMGPRAGIGEITLPPLQEGSSIMPGKINPVMCEYVE
                     GIAIDILGRDQAIAFYCSSGQLELNHLAPFIAHHILTMIEELKESIHVFAEKCIRGIK
                     ANQDRCNELLEKSFAMATCIVPHLGHERTSELVRECLKEGKIFKDFLIERQIFSEKEL
                     ESIIESSRSSQTLEYPELE"
     gene            2543..3796
                     /gene="hydF"
                     /locus_tag="PKN31_07080"
                     /pseudo
     CDS             2543..3796
                     /gene="hydF"
                     /locus_tag="PKN31_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890447.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
     gene            complement(3928..4152)
                     /locus_tag="PKN31_07085"
     CDS             complement(3928..4152)
                     /locus_tag="PKN31_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99723.1"
                     /translation="MTDDVDSRLKTSGVTKVRKECVKLRLSGLPGKNAGAEKRGAKHE
                     KGKCRNGFCAFIAIPGSYKGLYNWDAGKNT"
BASE COUNT         1277 a         1002 c         1041 g         1215 t
ORIGIN      
        1 gtcgctggga tgtattttcc cagcattatt gatttttagt ttttctatct cttttgcttt
       61 tatcttacgc cgtaggccac ctgctcctcg tgatggtagg aaaagaaagg agttcacaag
      121 agggcaacgc ccctttatat ttctgagtca tcaggaggga catttacccc ctgatattcg
      181 gttgctaata tttagcacca acttttaagt tttgaggtac aatgaggaaa acatctctgg
      241 ggggaaagtt atgaaagaag aaactatcgc ggggatattc gaaaacgagc ttgccggaag
      301 ggtcaaggaa tatcggctaa tcgattctca aacagtagtg gtggctgaat gggtacgatt
      361 caaatgccag tttggctgtg atggatacgc acagagtctt acctgtccac cgtattcgcc
      421 ggagccctcc cgcaccagaa aaatgctcga agaatacaga caagctgcta ttctctggaa
      481 accagaagat tgcgagaccc tgcgaaagat tgccgctgac ttagagagaa ctcttttcct
      541 ttccggatat tataaagcgc tggctatccc ctcaggaccg tgtgagctgt gtaatccctg
      601 tcccttagaa tatccctgtc tgcatcctga atatgctcgc ccttctatgg aagcctgtgg
      661 aatcgatgta tatgcaactg taaggcaaca tggtttcccg attgaggtgg ttcgctcaag
      721 aagttgccag cccaactact atgctcttat cctcatcgaa tagaagaaga caggaaaaat
      781 gaccaccttc ccttgctctg aggctgggtt tcagctatta tcagataaga gaatttgact
      841 ggtcgttttg ctatttatcg ggggatacat gttcgtttac tgttaggggg ctattcctcc
      901 gtccctgaat caagacgaag tttctattga ctgttatgcc tggctcttct gtattatgat
      961 atcgatagaa acaccctggt tcgcactgtt tcccggggaa gcagtcacaa tgtgttttta
     1021 cgcctgctta ttaatttttg ccagtcaatt aaaataaaaa gagacctgtc tggttctcat
     1081 taacagggaa accggataaa aaggagtgaa aagatattgg aaaatagaaa ttttcgcagg
     1141 gaaagagatt ccctgggtga agagttaata cctcaaaatg catactgggg aatccattcc
     1201 ctgagagccc gggataattt tcctatctct caaaaagttc cttcttcgtt gataaaagcc
     1261 tattttgcag taaaatgggc ctgtgccaga gcgaataccg aaatcgggct tctggaggag
     1321 aacacaggta acgcaatact taaagcttgc gaagaaggaa tgcagggtaa attcgatgaa
     1381 gaaatcatcg ttcctatcct tcaaggtggt gccggaactt cgctgaacat gaatataaac
     1441 gaagtgattg ctaaccgggc tctggaaatc ctgggtcacc agagaggaag ctaccagatt
     1501 gtcaaccctt tcgatagtgt caacctgagc cagtcaacaa atgatacttt cgtaactgca
     1561 gtaaaagtgg cagtgctgtg gcagctcgat gaattagaaa aggaaataat ctttatccag
     1621 gaagagctcc agcaaaaaga aagagagttt gcctctatag ccaaggtggg acgaacccag
     1681 cttcaggatg ccgttcccat aactctggga atggaatttg gagcttttgc cgaagccatc
     1741 tcccgagacc gccggagact cgaaaagggc aaagaactaa tgaaagaaat taacttaggt
     1801 ggtactgctg taggaaccgg attaaatgcc caccctgaat acagggcaag agtcgttccc
     1861 tatctcaacg agattactgg attatctctc acgagagcaa caaatgcttt tgaaaacacc
     1921 cagaatttag atgttttttc tgaattatca ggtcttttac gttctctagc cgtgaacttg
     1981 cgcaaaatag ccaatgactt gcgcctgctt tctatgggac cacgagctgg cattggagaa
     2041 attaccctac ctcctctcca ggagggctct tcaattatgc ctggcaaaat caatcctgtt
     2101 atgtgtgaat atgtagaagg aatcgccata gatatattgg gcagagacca ggctattgct
     2161 ttttactgtt ccagcggaca gctggagcta aaccatcttg ctcctttcat tgctcaccat
     2221 attctgacta tgatagaaga gcttaaagaa agcatacacg tatttgcgga aaaatgcata
     2281 cggggaataa aggccaacca agaccgttgc aatgagttat tagagaaaag cttcgctatg
     2341 gcaacctgca tagtccctca cctgggacac gagcgaactt cagaactggt tcgggaatgt
     2401 cttaaagaag gtaagatttt caaggatttt ctaattgagc gccagatatt ttctgaaaaa
     2461 gaactggaaa gcataattga atcttctcgg agttcccaga ccctggaata tccggagtta
     2521 gaataaaagg agtaaaaagg ctatgacaac acaaactaca ccccggggtt tacgacctca
     2581 tatcgggcta tttggcagga gaaatgttgg caaatcttcc cttattaatg ctctaactca
     2641 acaagaagtg gccattgtat cagaaagtcc tggtactacc accgacccgg tattcaaggc
     2701 cattgaatta cttcccttcg gaccggtaac cctcatcgat accgcaggac tggatgacga
     2761 ggggttttta ggagaactcc gcaaaaagaa aaccatggag atactcaggc gctgtgattt
     2821 ggccctcgta atatgggatt ataactcaga ttttaccttt gaaaaggagc ttattgacct
     2881 tttgcgtagc aatggcacga ccataatagg ggtagagaac aaaattgatt gtccttctcc
     2941 aggcaaagct tttatagata aaaatatacc agtctttaga gtgagtgcca aaaccggcga
     3001 aggtattgcc gagttaaagc aagaattgat atctatatta cagagctctt atgaggagaa
     3061 gcctctcatt aaagacctgg tgtctcccgg agacatggtg gtgctggtag tgcctataga
     3121 cctcggggct cccaaggggc ggcttattct cccccaggtg cagaccataa gagacctgct
     3181 cgatgccgaa gctacagctg tagttaccaa agaaagggaa ttagcagaaa ctctgaagaa
     3241 gttaggcagc cctccccgta tggtggttac tgactctcag gtgtttcata aagttgccga
     3301 agtagtacct caggaagtgc cactgaccag tttttcaatt ctttttgccc gctacaaggg
     3361 agacctttga actctcgtag aaggagccaa aactatcgaa actttgaagc ctggagaccg
     3421 ggttttaata tcagaagcat gcacacatca ccctgtgcct gacgatattg gaagggtaaa
     3481 aatacctcac tggctacgcc aacgattggg atttgaaata aaaaccgaaa tcaatgttgg
     3541 agcaagcttt ccggataatc tttcacaata ccgattaata attcattgtg gcgcctgcat
     3601 gctgaacccc aaagaaatgt attatcgtat cttaactgcc aaaagagaag gtgtacccat
     3661 caccaactat ggaatagcca ttgcttacct gcaggggctc tttcccagag tgcttgaagt
     3721 ttttccagaa atgagagtaa agcctcaagg tacagagcct tccaccattg ccttccaccg
     3781 ggtagcaggg gaatagcctc tatagagtgt tttccaaaat ggaagttagc ggtcaggtct
     3841 tttctcttca tcagccaatt ggagattgaa aattgcgtta tctaaaagtc cgggcttttt
     3901 ctctcttgac gaggtaagtt tatcggttta ggtatttttc ccggcatccc agttatagag
     3961 ccctttgtag ctgccaggaa tagcgataaa agcacaaaac ccattgcgac atttcccttt
     4021 ttcatgcttt gctcctctct tctctgcccc ggcattcttt ccgggcaacc cgctcaaccg
     4081 tagcttcaca cattccttcc tgactttcgt tactcctgaa gtcttcaatc gggaatccac
     4141 atcatccgtc attcccgaaa ttttttatcg ggaatccaga ggcttttaaa accatggacc
     4201 ccggcttgaa gattgccggg gtgacggact tggggtgata ggagcattca ggagtgacac
     4261 cccctccgtc attcccgaaa tcttttatcg ggaatccaaa tgcttttaaa taatttagac
     4321 cccggcttaa aaattgccgg ggtgacggac ttggggtgat agaaacgttc aggggtgacg
     4381 aggaggtgtc attgcgagtt cgtggtttcg cttacgtggc aatcccaagt tttttactct
     4441 atctaatttg ctttttagtt ttttgaggct cagaaaagcg agatcgccac ctcaatcgct
     4501 cctcgcgatg acagaaaagg gagggtttcc tctcc
//
LOCUS       DAOPSS010000128         4438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_15879_length_4438_cov_6.386493, whole genome shotgun sequence.
ACCESSION   DAOPSS010000128 DAOPSS010000000
VERSION     DAOPSS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15879_length_4438_cov_6.386493"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PKN31_07090"
     CDS             complement(<1..1087)
                     /locus_tag="PKN31_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA99724.1"
                     /translation="MNSRERVKIALEHREPDRVPVTASFTPEFAQRLRNYFGLPSHFS
                     NPHGGEIHDLEETLGLDIIQYAVGIGNSFYASEEREYICDWGIRWRQVEYETPFGVGH
                     YTEIAEHPLAGDKLYQYVPPDPYREELYTPLGRVMEKYGEKYFILGVTVCTIFEAAWY
                     LRGMDQLLMDMIEDEEKANYVLDIPFHFHLEAAKRLVEMGVDGIWLGDDVGSQKGMLI
                     SPELWRKYLKPRMATICRELKNINPHLKIAYHSDGNIYPIIPELIEIGIDVLNPVQPR
                     AMDPAFLKERYGNKLSFWGTIDEQHTLPFGTTEEVEKETRERLKVMAPGGGFIISPTH
                     HVQLDTPLENFLTFLETVKKYGYYPLSF"
     gene            complement(1143..2279)
                     /locus_tag="PKN31_07095"
     CDS             complement(1143..2279)
                     /locus_tag="PKN31_07095"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOA99725.1"
                     /translation="MKKIVSLLVVLLLVWGVSAVAFAGEVEKSFWGNTKEGAPIYLYT
                     LTNDNGVMVQLTNYGATVVSLYVPDKEGNLGDVVLGFDSVSDYEKPDPYNPYFGATIG
                     RYGNRIAGGQFTLDEKTYTLAQNNGPNHLHGGLKGFKDQIFEAIPMKTPEGPAVRFKY
                     LSHDGEEGYPGNLQVSVTFTLTDDNELKIKYLATTDKPTVVNLTNHSYFNLAGEGSGD
                     ILDHELMIVADNYTPADDTLIPTGIAPVAGTPLDFTTPHKIGERIGELIREEGDDPFR
                     GYDHNFVLNNQTGELALAARAKESSTGRVMEVYTTEPGVQLYTGNWVNTRGKEGKLYG
                     QFSGFCLETQHYPDSPNHPDFPSTVLRPGEVYSTVTIYKFLVEE"
     gene            complement(2348..3496)
                     /locus_tag="PKN31_07100"
     CDS             complement(2348..3496)
                     /locus_tag="PKN31_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOA99726.1"
                     /translation="MRKKVTIKDIAQELNVSVATVSRALNGEKGVSPDLRKKIIIKAQ
                     NMGYFRWPSLNLLNGESKNMVVIFPENDYFWERVEKGVEDAQELLQHFGIEIKVFRTA
                     GHNLTQQNQLLENILQKGNIDGLALVPADFTQLDYFINAFYLQNVAVATFNVDAPLSK
                     RLFYVGQNLNQAGKATGRIFNNLLNDKEGKISILTSNKRAPSHMGRWQGLIDFVHKKG
                     LPLDVSQVVEAKDDEEAYQITKDKLLEEKDLLGIFVSTAFGNIGVGKALKEKGKKDLV
                     VVGNDLGEGWIPYIKEGIIDCCLFQAPYLQGYLTLVFLAKFLLFEENPPNKNIFLPII
                     PVFSENLDGDLLSFEQCVTRHASLSYQFSEKDTREEFSLSIAEQKEVR"
BASE COUNT         1252 a         1032 c          847 g         1307 t
ORIGIN      
        1 aaaaactgag gggatagtaa ccatattttt tgaccgtttc taaaaaagta aggaaattct
       61 cgagaggagt atccaactgc acgtgatggg tgggagaaat aataaaacct cctcccggag
      121 ccatcacttt cagtctttcc cgggtttctt tttctacttc ttctgtggtt ccaaaaggaa
      181 gagtgtgttg ctcatcaata gtcccccaga aagagagctt gtttccatag cgctctttca
      241 aaaaagcggg gtccatggcc cggggctgca cagggttgag aacgtctata cctatttcta
      301 ttaattcagg aataatgggg taaatatttc catcgctatg ataagcgatt tttagatggg
      361 gattgatatt ttttaattcc cggcagatgg tagccattcg gggtttcaag tattttcgcc
      421 acaactccgg agaaattaac atccccttct gcgagcccac atcgtctccc aaccagatac
      481 catcaactcc catttccacc agccttttag ccgcctctaa gtgaaaatga aaagggatat
      541 ccagaacata attggctttt tcttcatcct ctatcatatc cataagtaat tgatccatcc
      601 cccgcaaata ccaggcagcc tcaaaaatag tgcataccgt aactcccagt ataaaatatt
      661 tctcaccata tttttccatc acccttccta aaggagtgta aagctcctct cggtaagggt
      721 cgggaggaac atactggtag agcttatcac cagctaaagg atgttcagct atttcagtat
      781 agtgacccac tccaaaggga gtttcatatt ctacctgccg ccatcttatt ccccaatcgc
      841 agatgtattc cctctcctct gaagcgtaga aagagttacc tatccctact gcatactgaa
      901 ttatatccaa tcccagagtt tcttctaaat cgtgaatttc tcctccatga ggattggaaa
      961 agtgagaagg taagccaaaa tagtttcgca ggcgctgggc aaattcagga gtaaaactgg
     1021 ctgtaaccgg cacccggtct ggttcacgat gctcgagagc aattttcact ctttccctgg
     1081 agttcatccc acctctcctc cattcctccc ctgcctcatg gggcagggga ggaggatttg
     1141 acctattctt ccaccaaaaa cttgtaaata gttactgtag aataaacttc acccggtctt
     1201 aaaaccgtgg aggggaagtc gggatgatta ggagaatcag gatagtgctg agtctccagg
     1261 caaaaaccgg aaaattgtcc atagagcttg ccctctttcc cccgagtgtt cacccaattt
     1321 ccagtgtaaa gctgcactcc aggttcagta gtgtaaactt ccattacccg gccagtgctg
     1381 gattcttttg ctcgagcagc taaagccaac tctccggtct ggttattgag cacaaagttg
     1441 tgatcataac ctctaaaagg atcatcgccc tcttcccgaa tcaactcccc aatccgctct
     1501 cctattttgt ggggagtggt aaaatcaaga ggagttcccg ctactggagc aattcctgta
     1561 ggaataagcg tatcatcggc aggcgtatag ttatcggcca caatcattag ctcgtggtct
     1621 aagatatcac ctgaaccttc tccggcaagg ttgaaataac tatgattggt taaattaact
     1681 actgtaggtt tatcggtggt ggccagatat tttattttca attcgttatc gtcggttaat
     1741 gtaaaagtaa cgctcacctg caagttgccc ggatatcctt cttctccatc atggctcaaa
     1801 tatttaaacc ggactgccgg gccttcggga gttttcatag gaatggcttc aaatatctgg
     1861 tccttaaatc cttttaaacc tccgtgcaaa tggttaggac cattattctg ggccagagta
     1921 taagttttct catccagagt aaattggcca ccggcgattc ggttaccata gcgccctatt
     1981 gtagcaccaa aataaggatt ataagggtcc ggtttctcat aatcggatac actatcgaaa
     2041 cctaacacta catcacctaa gttgccttct ttatccggga cataaagaga tactactgta
     2101 gccccgtagt tggtcaactg caccataact ccattgtcat ttgtgagagt ataaaggtat
     2161 ataggtgctc cttcttttgt gtttccccag aaagactttt ccacttctcc agcaaaagcc
     2221 acggcactaa ccccccacac cagtaacaaa accactaaca aactgactat cttcttcatg
     2281 atctactttc ctcctttaaa gcattttaac tttgcacaag ctaaaaccca gaatatagtt
     2341 aactttccta cctcacctcc ttttgttcag caatagacaa cgaaaattcc tccctggtgt
     2401 ctttctctga aaactgataa cttaaagaag catgtcttgt tacgcattgt tcaaagctca
     2461 aaaggtctcc atccagattt tcggaaaaaa cgggaataat gggaagaaaa atgtttttgt
     2521 tgggaggatt ttcttcaaat agcaggaact tagccaaaaa caccagagtt aaatatcctt
     2581 gcagataagg agcctgaaaa agacagcaat caatgattcc ttctttgatg tagggaatcc
     2641 atccctctcc caggtcgtta cctaccacca ccagatcttt tttccctttt tcttttaaag
     2701 cttttcccac tcctatattg ccaaaagcgg tagaaacaaa aatacccagt aaatcttttt
     2761 cttcgaggag cttatcttta gtaatttgat aagcctcttc gtcatcttta gcctccacta
     2821 cctgagaaac atccaaagga aggcccttct tgtggacaaa atcgattaat ccttgccatc
     2881 gacccatatg ggaaggggct cttttgttac tggtcaagat gctaattttc ccttctttat
     2941 cgttcaagag attattaaat attcttcctg ttgctttgcc tgcctgattc aagttttgcc
     3001 ctacatagaa aagcctcttg ctcaaaggtg catccacatt aaaagtagcc accgctacat
     3061 tctggagata aaaagcgtta atgaagtaat ctaactgggt aaaatcagcg ggaacgaggg
     3121 ctaaaccatc tatgtttcct ttttgcagaa tattttccaa caactggttt tgctgagtca
     3181 aattgtggcc ggcagtgcga aaaactttta tctctatccc aaagtgctgt aataattcct
     3241 gggcgtcctc gacacctttc tcaactcttt cccagaagta atcattctcg gggaaaataa
     3301 ctaccatatt tttagactct ccatttaata gattaagaga gggccagcgg aaatagccca
     3361 tattttgagc ctttattata atctttttcc gtaaatcagg actaactcct ttttcaccgt
     3421 ttaaggcccg gctcacagta gctactgaaa catttagttc ttgagctatg tccttgattg
     3481 taactttttt tctcacactg taatccttaa gataaatttt tactccaaaa taataacact
     3541 atcctaaaaa tctgtcaaat tcttttatca ctacgctacg gaaaaggtcg gtatgttaca
     3601 aagtcccttt gttttcccgt ccgccacagt taactgcaaa aaccacggca acctctatac
     3661 tatgcgctct ctcctcttat ataggttttg atatcgcgat aaatcatcac aaaccccacc
     3721 tcgcaacctg acaaaagcgc aagaaattat agtgcctgct aaatcgctca agacttgaaa
     3781 attaattaca ttaggtaaaa aattcggaat atcaaggctg gagaaggcat gatatcgtgt
     3841 gcggtgattt ttcttttctc gttttctaag gagaagaaaa actaccctaa ccccggaagg
     3901 gtgagggaaa gaaaaaaaga atcaagagag gaagaatatg tggagtctct cgtcaggact
     3961 tcctcctctc tcattcccga aatttagccg ggaatccacc ccctccgtca tgcctgagcg
     4021 cttttatcgg gaatccaaat acttttaaaa accatggacc cctgatagaa ccattcaggg
     4081 gcaggctaaa aacggcttga agattgccgg ggtgacgagg agatgggaat ccaaagtttt
     4141 ttgcccttaa ataatttaga ttcctgatag aaccattcag gaatgacaga taaggtgatg
     4201 atagaggcct tcaagggtga cattccttct ttgtcattcc cgaaatcttt tatcgggaat
     4261 ccatgttttt tcttttatct tttgtctttt tgtgtttttg tcttttgggg ttatgagggg
     4321 gataaatcct cctcatagtc atgataggct ttgcgggaga gtcacagagt gctaaaacca
     4381 ggggggtttt tgattctggg ctttatcgac cgggatagct ttataggaca aaaatact
//
LOCUS       DAOPSS010000129         4437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_15882_length_4437_cov_7.456413, whole genome shotgun sequence.
ACCESSION   DAOPSS010000129 DAOPSS010000000
VERSION     DAOPSS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15882_length_4437_cov_7.456413"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..1284)
                     /locus_tag="PKN31_07105"
     CDS             complement(346..1284)
                     /locus_tag="PKN31_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99727.1"
                     /translation="MNKNKSFFRNQTFFLITVIIGLSLLFGIINPRFLSKGNIFAIFQ
                     QIAVLGICTMSMTLLLVSGGIDLSIGSMIGLSTVIICTLVMNGTNVYVAVLLGFSTSV
                     ICGLINGIIISKSKCIPLIVTLGMSYVYYGLALVISQGLFLTLKGKFQFLGRGRVLGI
                     PIPMIILILMVLGTHFLLKNTKYGRRIVAMGGNEQMAFLSGINVDRLKIINYTLSGAI
                     IGVASLVLVSRLGNVLANVGEGYELRALASAIIGGVTFEGGRGTVLGAFLGVILMGII
                     QNGLNILNVSSYFQTFTIGIIIVIAVIVSNIEKIRK"
     gene            complement(1277..2779)
                     /locus_tag="PKN31_07110"
     CDS             complement(1277..2779)
                     /locus_tag="PKN31_07110"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA99728.1"
                     /translation="MDPIIQTQNIVKTFPGVMALAGISFDLYPGEIHCIVGENGAGKS
                     TFIKILSGFLRPDQGEIQIRDKKYRFLTPHTAQSLGIQVVYQENILVPQMSVAENVFV
                     GRELSSPWGMVNHRKTIEEAQKIIDSLEIDLSAQEAVENLSVAEQQFVKIVRALAFEP
                     QVLIMDEPTAVFNVKDAQIVLNLARSISKRGIGIIYISHRLEEIIDLADRITVFKDGK
                     KVACHDNREEKVDIPTITKEMVGRDINLFYRKEKKAVEKPILEVRGLRLSEASPSISF
                     SLHEGEILGVAGLVGSGRTEIMKALFGAGKKFSGEVFYRGEKINITSPQEAIKIGMGF
                     ITEDRQKTGLAMGLSIIKNITLPSLDDLFPGYLIPLPQEARIVENYVQQLDIKTPSLE
                     QEVRFLSGGNQQKVVLARWLLKEAEIIIFDEPTVGIDVNAKNEIHELIANLAQEGKSI
                     IMISSEMPELIALSDRVIVITRGAISAELKEGEITEENIIFHAFGVNTNE"
     gene            complement(2961..3965)
                     /locus_tag="PKN31_07115"
     CDS             complement(2961..3965)
                     /locus_tag="PKN31_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOA99729.1"
                     /translation="MKKYRRLVSILSLVLVFLTVVGTQVVFAQEGKKLVFGNIAFSRK
                     DVWNNYSILAFEYAARQKGVETIVLDPEGNLEKAVAAMEDLIAKKVDGVSVFTITPEL
                     DVTLYEMAKEAGIPITFENSLPADTSLDYISCVACQYEDIGYAAGKFISENYPGSKLF
                     YVMGAPGMGITELYEQGLHQALEEFGTVELVGTQPTDWGAEQAMNVTQNAIQSGLEFD
                     VIFANNEQMATGVINALKDAGLFGKVKIVATGGGPHGLEMIANGELDATMSAPVSLQG
                     LITFKNLYEYVAKGKKPEKFIPLPIIPITKENLSEAISWEISDENYERAVEYIGGIDD
                     "
BASE COUNT         1279 a          958 c          852 g         1348 t
ORIGIN      
        1 gtggatgcct gataaaagcg ctcaggcatg acggatgatg tggattccca attactaatc
       61 tctggaatag cagagagggg ttgttcccga gcaagaaaag acaggttgct acgggaagcg
      121 agcgactttc acaaccactt tggggaatta tcacgctgcc gatgcaacga ggtgtgagat
      181 ggcatatggg ggagcgatct ctctttttga gtctcaataa actgctaaaa agcaagttgc
      241 atcaggttag agatttaggg tcatgatggc tccccggggt gagattgccc gtaataatgt
      301 aataatatct tggcatgaaa aataaggaga gtgcaaaaaa ttctcctact ttctaatttt
      361 ttcaatattg ctcactatta ctgctattac aatgataatc ccgatggtaa aggtctggaa
      421 ataggaggag acatttagaa tatttaaacc attttgtata attcccatca atattactcc
      481 caggaatgct cctaaaactg tgccccttcc tccttcgaaa gtaactccac caatgatggc
      541 ggaagccaaa gcacgtagct cgtatccttc gccaacatta gctaacacat tccccagccg
      601 ggagaccaaa actaaagaag ctactcctat gatagctccg cttaaagtgt aattaattat
      661 tttaagcctg tctacattta tgcccgagag aaaagccatt tgttcatttc ctcccatggc
      721 tactattctc cgtccgtatt tggtattttt caagagaaag tgagtgccta aaaccattag
      781 aatcaggatt atcataggaa tggggatgcc taaaactctt cctctcccca gaaattgaaa
      841 tttccctttg agagtgagaa acaaaccctg ggatataacc agcgccaatc cataataaac
      901 atagctcatg cctaaagtta caatcaaagg gatgcatttg cttttactta taattatgcc
      961 gtttattagt ccgcaaatta ccgaggttga aaagcctagg aggactgcta cataaacatt
     1021 agttccattc attactaaag tgcagataat aactgtgctc aggccaatca tagagccaat
     1081 ggagagatct attcctccgg atacgagaag aagagtcata gacatagtgc aaatgcctaa
     1141 aacagcaatc tgctgaaaaa tggcaaagat gttaccctta ctcagaaatc gaggatttat
     1201 gattccaaaa aggagagaaa gaccaatgat tacagttata aggaaaaagg tttggtttcg
     1261 gaaaaaagat ttattcttat tcattagtgt tcactccaaa agcatggaat atgatatttt
     1321 cttcagtgat ttctccttct tttaattctg ccgagatagc gcctcttgtg atcactatta
     1381 cccgatcgct cagagcgatc aactccggca tttctgaaga aatcatgatg atggatttcc
     1441 cttcttgagc taaattagct attaactcat ggatttcgtt tttagcattg acatcaattc
     1501 ctacagtggg ttcatcaaaa ataatgattt ctgcttcttt caaaagccag cgggctaaaa
     1561 ctactttttg ctggtttcct ccacttaaaa atctcacttc ttgttctaag gaaggagttt
     1621 tgatatccag ttgctggaca taattttcta ctatccgggc ttcctgagga agaggaataa
     1681 gatatccagg aaataagtca tctaaactgg gaagggtgat gtttttaata atggagagac
     1741 ccatagccaa tccagttttc tgacggtctt cagtgatgaa gcccatccct atttttattg
     1801 cttcttgggg agaagtgatg tttatttttt ccccccgata aaagacctca ccggaaaatt
     1861 ttttccccgc tccaaacaga gctttcataa tttctgttct tcccgaaccc actaacccgg
     1921 ctactcctaa tatctctccc tcgtgaagag aaaaagaaat ggaaggagag gcctcactca
     1981 ggcggaggcc tctcacctct aatataggtt tctctaccgc ctttttttct tttcggtaaa
     2041 acaggtttat gtctcttccc accatttctt tagtgatggt aggtatgtct actttttcct
     2101 ctcgattatc atggcaggcc accttttttc catctttgaa tacagtgata cggtcagcta
     2161 aatctatgat ttcctccaga cggtgggaga tatatattat ccctatgcct cttttactaa
     2221 tactacgggc taaattcaaa actatctgtg catcttttac attgaacacc gcagtgggtt
     2281 catccattat tagcacctgg ggttcaaaag ccagagctcg tacgattttc acaaattgtt
     2341 gttcagcaac gctcagattt tctaccgctt cttgagcaga aaggtcgatt tctaacgagt
     2401 ctatgatttt ttgggcttct tctatcgttt tgcggtgatt taccattccc cagggggaag
     2461 aaagctctcg ccccacgaag acattttctg ccacactcat ttgaggaacc aggatgtttt
     2521 cctgatatac gacttgaatt cctaaagatt gagcagtgtg gggggtgaga aatcggtact
     2581 ttttatcccg aatctggatt tctccctgat cgggcctgag aaacccggat aaaattttta
     2641 tgaaggtaga tttccccgct ccattttctc cgacgataca gtgaatctct ccaggataaa
     2701 gatcaaaaga aataccagct agagccataa ctccaggaaa agtttttaca atgttttggg
     2761 tttgaattat aggatccatt ggttgttcct gtgtcctttt ctttctgtag agagattttt
     2821 tcctcctggg tctttgctat ccttacttgt ataccaactc actatagtgg ttactccttc
     2881 aaggaaatgc ttttgagtgt accaaagtga aaaaaggctt ccctcgaggg aagccttttt
     2941 tcactaaagc ctaaagcttc tcagtcatct atgccgccaa tatactccac ggctctttca
     3001 tagttttcat cactaatttc ccaggaaata gcttcagaaa gattttcttt ggtgataggg
     3061 atgataggca aaggaataaa cttctccggt tttttgcctt tagctacata ttcataaagg
     3121 tttttaaaag taattagccc ttgcaaagat actggagcgc tcattgtggc atccagttcc
     3181 ccattggcaa tcatttccaa accgtggggt cctccgccgg tagcaactat ttttactttg
     3241 ccaaataatc ctgcgtcttt caaagcattt ataactccag tagccatttg ttcattgtta
     3301 gcgaaaataa catcaaattc taagccggac tggatagcgt tttgggttac attcatagct
     3361 tgttcggctc cccagtcagt gggctgagtt cccaccagct ctaccgttcc aaattcttcc
     3421 agtgcctggt gcaggccctg ttcataaagt tcggtaatgc ccatccctgg ggctcccatc
     3481 acataaaata atttgctacc cggatagttt tcactgataa actttccagc agcgtatcca
     3541 atgtcctcgt actggcaggc aacacaagaa atataatcca gggaggtatc agcaggaaga
     3601 gagttctcaa aagtaatggg gattcccgct tctttagcca tctcgtataa ggtgacgtct
     3661 aattctggag ttatggtaaa tactgaaaca ccgtctactt ttttggctat taagtcttcc
     3721 atagctgcta ctgctttttc taggtttcct tccgggtcta aaacaatagt ttctactcct
     3781 ttttgcctgg cagcatattc gaaagccaaa atagagtagt tgttccacac atctttacgg
     3841 ctgaaagcaa tgttaccaaa aactaatttt ttaccctcct gagcaaaaac cacctgagtt
     3901 cccaccacag tgagaaaaac aagaaccaaa gacaaaattg atactaaacg acgatacttt
     3961 ttcattaacc attcctcctt ttaagttatt ttgttgtcta tccagcttcc taatttatac
     4021 cacataaaga gataataaaa aagcccccat ttcaaaaaca aagccttaat aagcgagaga
     4081 aggtcagatt ctgtaagaaa aggaagaatt caataatttt tgtagcctgc tatgatttct
     4141 ctaactgtta gaaagatgga ggccggatag aaaattttaa atggaggcac aaaatccatt
     4201 ttgcctacat cgggttaaaa actcgggatt gctaaccgta cgctggattt ttctcccagc
     4261 gtcgttgttt gcttttttac gccgtcaggc tatcaagcga ggatcgtatg accctttgag
     4321 agagtcttac agagtgaagg atggtgcaat ctttctttta tactcaagcg aaaatctaac
     4381 ctcaccagga ttgaaattac accgggaaaa ataccccagt atattttgtt aagttag
//
LOCUS       DAOPSS010000130         4435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_15889_length_4435_cov_9.086986, whole genome shotgun sequence.
ACCESSION   DAOPSS010000130 DAOPSS010000000
VERSION     DAOPSS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15889_length_4435_cov_9.086986"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKN31_07120"
     CDS             complement(<1..239)
                     /locus_tag="PKN31_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA99730.1"
                     /translation="MRKRKLGWTDLELTVIGFGSWALGGGGWESGWGPQDDEESIAAI
                     HRALDLGINWIDTAAVYGLGHSEEIIAKALRGMSE"
     gene            607..1596
                     /locus_tag="PKN31_07125"
     CDS             607..1596
                     /locus_tag="PKN31_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA99731.1"
                     /translation="MKKYRFSIVLALVFLLVFALPVFAQEKYKIAFVPKLVGIPYFNA
                     MEEGGKKAAEDLGVEFIYTGPVTADVAKQIETIDNLITQGVDAIAVAPNDPAAITPVL
                     EKAKEKGILVLTSDTDGAQEVRDLFVNQAIEKEIGYTTMDELAKAMDYEGEFAIVSCG
                     PTAWNLNTWILYELERLAEYPDMELVTIRYAEEDVQLAINVALDLINAFPNLKGIIGQ
                     CSTSAPGVAEAVTQAGKIGEIVATGVSVPSMMRPYVKNGAVKSFVLWNPVDLGYLTVW
                     AAKYLLDGNQFEEGIEYEVPGISTKPRYAAESKMLILGPPMVFDASNVDDFDF"
     gene            1666..3183
                     /locus_tag="PKN31_07130"
     CDS             1666..3183
                     /locus_tag="PKN31_07130"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA99732.1"
                     /translation="METTILECREISKSFGGVKALKKVDFSLRQGEVHALVGENGAGK
                     STLIRIISGALLPDEGEIWYGGQKVEITSPRLAQGMGISAVYQDPLIYPYLSVVENIF
                     LGREIKTPRGNIDWKRQEEKAQELFASLNIAPHFLRQPMGELSVGLQQLVLIAKALAY
                     ESQVIIFDEPTAILTEHEAERLFQIIETLREQGVGIIYISHRLEEIFQIADRVTVMRD
                     GEVVGDYPLSAVNREMIVELMAGKHLVEEIEHGGEKSEQPVLVVKNLTKKGKFEDISF
                     EAFQGEILAFSGLVGSGRTDVAQAIFGIISPDSGEIYYRGEKFKVHSPRHAMEQGIAY
                     LPEDRKSQGLFPILSVSYNITVSILRQITNHLGIVNTRKEREIAEKYVKELSIRTPGL
                     EAKVFSLSGGNQQKVVLAKWLGVNPRILILDEPTRGIDVAAKAEVHQMIAQLVERGIV
                     VIIISSELPEVLKLADRVIVMHEGKITGVFEGEDISSTNLVRAATGERVLPGKSD"
     gene            3199..4146
                     /locus_tag="PKN31_07135"
     CDS             3199..4146
                     /locus_tag="PKN31_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99733.1"
                     /translation="MINFQRIVRRREFTVLVLILILSAIFSFTTERFLSVSNITNLLL
                     QLSGVAITAIGMTMVIITGGIDVSAGSTLGMACVVSGYLLMGGYSPFLALLLAVLVGL
                     GIGFVNGAIIVWGDVPPIIATLGMMNLIRALMFQTLGGRWISGIPPACRTIGLGKWLG
                     VPIPMWIATFLIIVFSYFLSYRPLGRHIYAVGNNPEAARVSGVNLKSTIIFVYALTGA
                     LAGFAGLINAARTGIVQTNSGMGFELDVIAAVVLGGTSALGGRGTIIGSLLGAFLVGI
                     IKNGMVLLNVPALSEGLVIGALIIISVLADIIRARRETR"
     gene            4143..>4435
                     /locus_tag="PKN31_07140"
     CDS             4143..>4435
                     /locus_tag="PKN31_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99734.1"
                     /translation="MKRDIWDTIIGNRVFFLSVLFIILFILLSLTVPGFLNLYNLTNM
                     TQYGVELGLLALAETLVILSGGGGIDLSVGSIMSLVAIVIGVLIGRVGLSPSLA"
BASE COUNT         1211 a          760 c         1066 g         1398 t
ORIGIN      
        1 ttttcgctca tacctcttaa ggctttagct ataatttctt ctgaatgtcc taatccataa
       61 acagcggcag tatctatcca atttatgcct aaatctaagg ctctatggat agcagcaatg
      121 gactcctcat cgtcttgagg gccccacccg gattcccacc cccctcctcc taaagcccag
      181 gaaccaaagc ctatcacagt gagttcgaga tcagtccaac ctaatttcct tttgcgcatg
      241 tcagacctcc ttggatgatt ttttttaatt attatacctt ataacccaag tttgcttaaa
      301 agctacgagg agagcattct ttatcttttc aaattccaat ctctttttat ctttctaata
      361 cacagaggat ggctttgtgc tatctgaagc ttattgctct tatccggttt ccagtgtttt
      421 taatcacctt tttccaaaaa tgtttcctga ttggttagaa ttaatactta ttcattgcta
      481 aattgatagg aagtttttcc gattgttatg gaatttgagt aaatatcagg aaaaaacgga
      541 ttgacttatc taaagggtat attataatta atattaacca aatacatggt aaaggaggaa
      601 ggttttatga aaaagtatcg tttttccatt gttctcgctc tggtgtttct tttagttttt
      661 gctctgccgg tttttgctca ggagaaatat aagattgctt ttgtgcctaa gttagttggt
      721 attccttact ttaatgctat ggaagaagga gggaaaaaag cggcagaaga tttaggagtg
      781 gaattcatct ataccggtcc agtaactgcc gatgttgcaa aacagattga aactattgat
      841 aatctcatca ctcagggagt agatgctatt gctgttgctc ctaatgaccc tgcagctatt
      901 actccagttc tcgaaaaagc taaggagaaa ggtatcctgg ttttaacgag tgacactgac
      961 ggggctcaag aggtgaggga cttatttgtg aatcaggcta tagaaaaaga aattggctac
     1021 accactatgg atgagttagc taaggcgatg gattatgaag gggaatttgc catcgtttct
     1081 tgtggcccta ctgcctggaa cctcaacacc tggattcttt acgagctgga gaggttagct
     1141 gaatatcctg atatggaatt agttaccatt cgctatgctg aagaagatgt acagctggcc
     1201 attaatgtag ctttagacct tatcaatgct tttcctaatc tcaaaggtat tattggtcag
     1261 tgcagtactt cggctcccgg ggtagcagaa gcagtaactc aggcggggaa aattggagaa
     1321 attgtagcga ctggagtatc ggtgccctct atgatgagac catatgtcaa aaatggtgca
     1381 gttaaatcat ttgtattgtg gaatccagta gacctgggtt acttaaccgt gtgggcggcc
     1441 aaatatcttt tagatggtaa tcaatttgaa gaaggaatag aatatgaagt tcccggaatt
     1501 tctaccaaac ctcgttatgc agcagagagc aagatgctga ttttgggacc accaatggtt
     1561 tttgatgcca gcaatgtaga tgactttgat ttttagccta tagtttatga atcaactttg
     1621 gagagggtct ctggccctct cctttttatt atagggagtt gaaatatgga aactactatt
     1681 ctggagtgcc gggaaatttc caaatctttt ggaggagtta aggctttaaa aaaagtggat
     1741 ttttctctcc ggcagggaga agtacatgct ttagtgggag aaaatggagc cggaaaatct
     1801 actttaattc gaatcatatc tggagctctg cttcctgatg agggagaaat ctggtacgga
     1861 ggccaaaaag tagaaataac ttctcctcga ttggcccagg ggatggggat ttctgcagtt
     1921 tatcaggacc cgctgattta tccctatctt tcagtggtag agaatatttt cctggggagg
     1981 gaaataaaaa cgcctcgggg taatattgac tggaaacggc aagaagaaaa ggctcaagaa
     2041 ttatttgctt ccttgaacat tgctcctcat ttcttgaggc aacctatggg ggagctgagc
     2101 gtcggcctgc agcaattagt tttaattgcc aaagctctgg cctatgaatc tcaagtaatt
     2161 atttttgatg aacctactgc tattctcacc gagcacgaag ctgagagatt gttccaaatt
     2221 attgaaacat taagagagca gggagtggga ataatttata taagtcatcg tctggaagag
     2281 atttttcaga ttgctgatag agttactgtt atgcgggatg gggaagtggt aggggactat
     2341 cctctctctg cagtaaatcg agaaatgatt gtggaattga tggctggtaa acatctggta
     2401 gaagaaatag aacatggagg ggagaaaagc gagcaaccgg tactggtggt taaaaacctt
     2461 actaaaaaag ggaaatttga ggatataagt tttgaagctt tccagggaga gatattagct
     2521 ttttccgggt tagttggttc aggaagaact gacgtggcgc aagccatctt tggtattatt
     2581 tctccagata gtggagagat ttattaccga ggagaaaaat ttaaagtcca ttctcctcgt
     2641 catgctatgg agcaagggat agcttatctt cccgaggacc gcaaaagcca gggtcttttt
     2701 cctattttga gtgtttccta taacatcact gtttctattt tgaggcaaat aaccaatcat
     2761 ctcggtatag ttaataccag aaaagaaaga gaaatagcag aaaaatatgt aaaagagctt
     2821 agtattcgca ctcctggatt agaagctaaa gtgtttagcc taagcggagg taatcagcag
     2881 aaagtagttc ttgctaaatg gttgggggtt aatcctcgga tacttattct cgatgagccc
     2941 acccggggaa tagatgtggc agccaaagct gaagttcatc agatgattgc tcagttagtg
     3001 gaaagaggta ttgtggtgat aatcatttct tctgagttgc ccgaagtatt aaagttggcc
     3061 gatcgggtaa tagtgatgca tgaaggaaaa attaccggag tttttgaagg agaagatata
     3121 agttctacca accttgttag agcagctacc ggagaaagag tacttcccgg taaatccgat
     3181 tagtgggaag ggaaaaccat gattaacttc cagagaatag tgagaagaag agagtttact
     3241 gttttagttt tgattttaat tcttagcgct attttttctt ttactactga gagattttta
     3301 tcggtgagca atattactaa cctcttgtta caactatctg gagtggccat taccgctatt
     3361 ggaatgacta tggtaataat aactggagga atagatgttt cagcgggttc tactctgggg
     3421 atggcctgtg tagttagtgg atatcttctt atgggaggtt attctccctt tttagctttg
     3481 ttgcttgctg ttttggtggg attgggcatt ggctttgtga atggagctat catagtttgg
     3541 ggagatgttc ctcctattat tgctacttta ggaatgatga atttgatcag ggctttgatg
     3601 ttccagactc tggggggaag gtggatatca ggtattcctc ctgcttgccg aaccataggt
     3661 ctgggtaaat ggttaggtgt gcctattcct atgtggattg ctactttcct cattattgtt
     3721 ttttcctatt tcttgagtta tcgacccctg ggtcgtcata tttacgcggt gggcaataat
     3781 cctgaagctg ctcgagtatc aggagtcaat ttgaaaagca ctattatttt tgtttatgct
     3841 ttaactggag ctttagcagg atttgccggt cttatcaatg ccgcccggac aggtattgtt
     3901 cagaccaaca gcggtatggg ctttgaactg gatgtgattg cagcggtagt tttaggaggg
     3961 accagtgcct tgggaggaag gggaactatc atcggaagtc tactgggagc ttttttggtg
     4021 ggtattataa aaaatggaat ggtgctactc aatgtaccgg cgttgagtga aggcttagtg
     4081 attggagcgc taattattat ctctgtatta gctgacataa taagagccag gagagagacg
     4141 agatgaaaag agatatctgg gataccataa ttggcaatcg ggtgtttttc ctatctgtgc
     4201 tttttattat tttgttcatt ttgttatccc ttactgttcc cggtttttta aacctctata
     4261 acctgactaa catgactcaa tatggagtgg aattgggtct tttggctctg gcggagactc
     4321 tggtaattct ctccggggga ggaggaattg acctttcagt ggggtcaata atgagcctgg
     4381 tagctatagt tattggagtt cttattggta gagtaggatt gtctccttct ctggc
//
LOCUS       DAOPSS010000131         4426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_15934_length_4426_cov_8.324868, whole genome shotgun sequence.
ACCESSION   DAOPSS010000131 DAOPSS010000000
VERSION     DAOPSS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15934_length_4426_cov_8.324868"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(503..1693)
                     /locus_tag="PKN31_07145"
     CDS             complement(503..1693)
                     /locus_tag="PKN31_07145"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOA99735.1"
                     /translation="MIQLIKIALVFIVILISLRYKINLGVTLAIAALLLLLFSAVAPI
                     ESLKIVISTFGDYSNLNLMANIVCITILGNVLSYTGQLNLLVKSTQTLFKDPRLVSLA
                     LPALVGILPMPGGAIFSAPLVEASRTSPHTQGEILAFINYWFRHIWEYFLPLYPAILL
                     VSSLSGLSLPWIVIHHLPFFVLAIIGGWLVVRKMEVENSPDPPGKVNFYEIIQPFIPL
                     IPPVLGVFLGIPIWVGGMIGLILAIFLGKLSFSQIISAMFQNFPWSIMIDVAGILIFK
                     TAITQGGVLQPVVDFMISQNMPVILLGLVLAFFIALVSGLTSAFVGVSFPLILPLLSL
                     ASLEKAIPLLYIGGYLGIIFSPTHLCLILSCRYFKTDIEKVYRLLLWPSILVFSWGVV
                     WYFI"
     gene            1895..3694
                     /locus_tag="PKN31_07150"
     CDS             1895..3694
                     /locus_tag="PKN31_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOA99736.1"
                     /translation="MKRFVLSLIVSFCLGWLCFCSAVCAATFFQGIWIDVRSIPLTED
                     GIRDMVRRVHSANFNAILVESFYLGETIYPSPFLSSQGLPSQMSTFEEAGIDPLKIIV
                     EEAHRLSLQVHVWFDMFYVGLNEPGALLSRYPQWSAINRDGSTGYQQGNNHFFWICPE
                     HPEVKEFYSQLFQEVGKNYPIDGIHLDYLRFPDPTLADACYAPEHCQKFEEEYGVDPR
                     EISPLNQPAFYREWNKTRAQFLTDFVTYISEELRHSLPHLIFSCAVKPQGFPEELNPR
                     SLQDWPRWAELGLFDVFIPMTYSSRPPEFKGLLLWINTFLSSSAPFYSGIWGVNLSDH
                     SIAQEITIAREAKSQGVSIFAYPYLTDQTLSFLAAGPFHEKPSPPTIEELSVPRELPP
                     EVKPVSYYQENARRVTALYTGTPVVPDGVLEEIWESAHWQADFTLITGEGEASQDTRV
                     ALLYDSQNLYVAFFFEKNPDEVVFSVTQRDGPAFYDDSVEIFLDPGKSKSFYYQLVTN
                     LVGAQYDGSSLQGAAWNGNWKAGVMEENHLYSLEVAIPFSEITKEPPSGGEEWGINFY
                     RNNLKQEEFSAWSPTPGVYAVPSLFGTVEFSVP"
     gene            complement(4097..4172)
                     /locus_tag="PKN31_07155"
     tRNA            complement(4097..4172)
                     /locus_tag="PKN31_07155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4137..4139),aa:Gly,seq:ccc)
BASE COUNT         1265 a          870 c          957 g         1334 t
ORIGIN      
        1 gcctgaaatt tagcgaggca tccatgtttt ttaaatactg tggacccctg ataaatacat
       61 tcaggggtga cgacctcctt ctctcgtcat tcccaaaatc ttttattggg aatccatatg
      121 tttttactgg gggaggattt tcccaccagt ggcatttact ttttaggttt ttgttttttt
      181 gctttttagg gtgatagggg gtttcccctt gtagtttgga tttctgataa atacgttcag
      241 gaatgacaaa cttgtgggaa tttcactctc actttcaccc cggaagagcg atggaaaaga
      301 gggcttttaa agagaggaag agaaaggaat ccgggtgagg cttcctttta cctttttgct
      361 ctgatttttt tctcaatcgc aatttttaca ttacatcctc gcagtattcc tgataacctg
      421 ttctttcact ctttagagag attatggcat gctattttaa tttttttggt atttgtactt
      481 tattatgcac cacggtctcc tactatataa aataccagac tactccccag gaaaaaacca
      541 ggatactggg ccagagaagc agtcgatata ctttctctat atcagtctta aaatatcggc
      601 aggaaagaat gagacagaga tgggtgggag agaaaataat tcccagatat cctccaatat
      661 agaggagagg aatcgctttc tctaaagaag caagagacag aagaggtaaa attaaaggaa
      721 aactaactcc tacaaaagcc gacgttagcc cgcttacgag agcaataaag aaggccagca
      781 ccaaacctaa gaggattacc ggcatattct gagaaatcat gaagtctact actggctgaa
      841 gaacaccgcc ttgagtaatg gcggttttga atatcagaat tccagctaca tcaatcatta
      901 ttgaccaggg aaaattctgg aacatagcgg agataatctg agaaaaagat aatttgccaa
      961 ggaaaatagc caggattaac cctatcatgc ctcctaccca gattggaatt cctaaaaata
     1021 ctcctaaaac cggaggaatt aaaggaataa aaggttgaat aatctcataa aaattgacct
     1081 ttccgggtgg gtcgggggag ttttctacct ccatttttct aaccactaac cagcctccta
     1141 taatagccag aacgaaaaaa ggaaggtggt gtattactat ccagggaaga gacaaacccg
     1201 atagagaaga gactaaaaga attgctggat aaagaggaag aaaatattcc cagatatggc
     1261 gaaaccaata attgatgaaa gctaaaattt ctccttgtgt atgaggtgag gtgcgagaag
     1321 cttctaccaa tggggcagag aaaatagctc cacctggcat agggagaatt cccacgagag
     1381 caggaagggc cagagaaacc aatcgggggt ccttaaaaag agtttgggtg gattttacca
     1441 ataggtttag ttgaccggtg taggaaagaa cgtttcccag aatggtaatg catactatgt
     1501 tggccattaa attgagatta gaataatccc caaaggtgct tatcactatc ttcaggcttt
     1561 cgatgggagc aacggcagag aaaagaagaa gtagaagggc tgctatggca agagtcaccc
     1621 ctaaatttat tttatatcgt aaagaaatca aaattacaat gaagaccaaa gcgattttta
     1681 taagttgaat caagaattag ccctcctcca ctgttaagtt cactttctcc gattatacaa
     1741 ctaattcctt tatttgagtt tatatcttta gaatcagaga tttagtaaga agaggggtaa
     1801 ggccttcgcc ctcaattttt ggggaaaact tcttctctaa gagaaagtcg gtaccgagat
     1861 ggcattttta gtgcttcttt gagataatac ggccatgaaa agatttgttt taagtttaat
     1921 tgtaagtttt tgtctggggt ggctttgttt ttgttccgcc gtttgtgctg ctactttctt
     1981 tcaaggaata tggatagatg tgcggagcat ccccctgacc gaggacggca ttcgggatat
     2041 ggtcaggcgc gtgcattcgg ctaatttcaa tgctatttta gtggagtcct tttatttggg
     2101 tgaaaccatt tatccttctc cttttttatc ttctcagggg ttgccttctc aaatgtccac
     2161 tttcgaggag gcgggcattg accctttgaa aataattgtg gaagaggctc atcgtctttc
     2221 tttacaggtg cacgtgtggt ttgatatgtt ttatgtggga ctcaacgagc ctggagcctt
     2281 actttctcgc tatccccagt ggagtgccat taaccgagac gggagcactg gttaccagca
     2341 aggaaataac cactttttct ggatttgtcc ggaacacccg gaggtgaagg agttttattc
     2401 tcagcttttc caggaggtgg gcaaaaatta ccccatagat gggattcatc ttgattatct
     2461 tcgttttccc gacccgacgt tagctgatgc ttgctacgct ccagaacatt gccagaagtt
     2521 tgaagaggag tatggcgtcg acccccggga aattagcccc ctgaaccaac cggcttttta
     2581 tcgggagtgg aataaaacac gcgctcaatt tttaacagat tttgtgactt acatttcaga
     2641 agagctgcgt cattcgttgc ctcacttaat tttctcctgt gcagttaaac cccagggttt
     2701 tccagaagag ctgaatccca ggtctttgca agattggcct cgctgggcag aactggggct
     2761 ttttgacgtt tttatcccta tgacttatag ctccagacct ccagagttta agggtctatt
     2821 actctggata aacacttttc tttcttcttc tgcgcctttt tatagcggaa tctggggagt
     2881 taatctttct gaccattcca ttgctcagga gattaccatt gctcgagaag caaaatcgca
     2941 aggagtatcc atttttgctt atccttatct taccgaccaa accttgagtt tcctggctgc
     3001 tggccctttc catgaaaaac cttctcctcc caccattgag gaattatcag ttcctcgaga
     3061 acttccccct gaagttaagc ctgtatctta ttatcaggag aacgcccgac gagtaactgc
     3121 tctttatacc ggtactcctg ttgtacctga tggtgttttg gaagagatat gggaatctgc
     3181 ccactggcag gccgatttta ctcttattac tggagaagga gaagccagcc aggataccag
     3241 agtggctctc ctttatgact ctcaaaactt atatgttgcc tttttctttg agaaaaatcc
     3301 tgatgaagtt gttttttccg ttactcaaag agacggaccg gcattctatg atgactcggt
     3361 agaaattttt ctggacccgg gtaagagtaa aagtttctac tatcagctgg taaccaatct
     3421 ggttggtgct caatacgatg gttcttcttt gcaaggagca gcttggaatg gaaactggaa
     3481 agctggagtt atggaggaga atcatcttta ctctctggag gtagctattc ctttttctga
     3541 aataaccaaa gagccacctt ctgggggaga agagtggggg ataaattttt atcgcaacaa
     3601 tttaaaacaa gaagaattca gtgcctggtc gcctactccg ggtgtctatg ctgtgccttc
     3661 cctttttgga actgtagaat tttctgtgcc ttaaaaaaca tctatgggga gttatcttgt
     3721 tagaagataa ctccccatag atgcaaggag gggattttct aagtttataa cttttcaatt
     3781 actcattttc agtccgaagg gtattatagc tggttattat tttttgtcaa tagttttttt
     3841 ggaaaattaa taaaatattt aataaatatt ttataatgat agcctccaac atagaaaaag
     3901 atgtgaggag tgcgtggtct caagccttca acttgtttta gcaaagaaat tcctcaaaat
     3961 actcctccta ggttttaggt ttagctaatt ttaaaacctt ccccattttg aatccctttt
     4021 ttataagtaa cggcttcagg agttaggaaa ggcaaaatga aaagtaaaaa tggctgaaat
     4081 tagtttggaa aaaaaatgga gcggaaaacg ggatttgaac ccgcgaccct cggcttggga
     4141 agccgatgct ctaccacctg agctatttcc gctcttatac actaatataa taacatgaag
     4201 aatgtttttt tcaagtatcc gaattgtctc aactaaaaat ctcgatacaa atcattctat
     4261 ttgcaggcta tcctccttaa gctgaaggtg gaatagggct cgcattatag tttatagttg
     4321 actacaagaa agtaggtaag ggaagggttt ttgagagaag cattttttaa gaaaaatcac
     4381 cctcacccta cccctctcac tccgagggct tctctttttg tcatgc
//
LOCUS       DAOPSS010000132         4417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_15972_length_4417_cov_8.169188, whole genome shotgun sequence.
ACCESSION   DAOPSS010000132 DAOPSS010000000
VERSION     DAOPSS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15972_length_4417_cov_8.169188"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKN31_07160"
     CDS             <1..272
                     /locus_tag="PKN31_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOA99737.1"
                     /translation="NWVELWEKLSYNPALFLGLDNKGILEEGRDADITIVDPGTRKRV
                     EVSNFESQGKNCPFENWSLPGWPVYTIVDGRIVMAEGSIVESEFC"
     gene            307..1179
                     /gene="pyrF"
                     /locus_tag="PKN31_07165"
     CDS             307..1179
                     /gene="pyrF"
                     /locus_tag="PKN31_07165"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOA99738.1"
                     /translation="MAVGLDPHFQLLPPYLWEKWVEKEGKTWEALSHCVLEFNSLILQ
                     ALSPVVGFAKIQVAFYEALGVPGMAVLKKTIEEAQKKDFIVILDGKRNDIASTATFYA
                     QGYLSQLEVGRDSCLPSFWDVDAITVNPYLGEDSLQPFFEEAKKEDKGVFVLTRTTNP
                     SAPFIQDVGEEKVFIRVARLVEKWGEGVRGESGFSSLGIVVGATYSEDMRFLREEFPS
                     LLFLVPGVGAQRGDFSSLRFAFREDGEGALVTISRDIIFSYLKEKSNEGWGFEEKARD
                     RAIFYQEKLREILK"
     gene            1176..1946
                     /locus_tag="PKN31_07170"
     CDS             1176..1946
                     /locus_tag="PKN31_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOA99739.1"
                     /translation="MSEAVIEEKKCWGERFVQLRLKEEEIARCARPGQFVMVRVQEGF
                     DPLLRRPLAVMKVEESSFYLFWEIVGRGTKILSSLNVGSKLEVLGPLGRGFSLFPSQA
                     LIVAGGRGITPLYFLASHFKENKIPFTFFLGVKDKEELFMVDFLQDFPGAYFWSCEEP
                     VEGGFCGTVLSLFHTWLEDKSFSPGTGVYACGPEGMLKNLYREGKTQNFPLEVSLETF
                     MGCGFGACMSCAVEKSNGKGYFHVCKDGPVFLAQELKW"
     gene            1943..2848
                     /locus_tag="PKN31_07175"
     CDS             1943..2848
                     /locus_tag="PKN31_07175"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOA99740.1"
                     /translation="MNLAVSLGKLNLKNPVILASGTCGYGREISSYLDLNKVGAITVK
                     GLSYYPFSGNPPPRLREVYAGVLNSIGLENKGVYDFIQEDLPFLSSFDTRLLVNIWGE
                     TANEFVQVAQELDRFERVDALELNVSCPNMERGGAVFCSDLEILKSIVGGVRDATRKP
                     LIVKLGPEVKEWGKTLDILENEGADILSLTNSFPALFVDVGEMDFFFKRKFAGLSGPA
                     IKPLALKMVYEVVNYSSLPVIGMGGIVSADDALEFLLVGAKAVALGVANFVDPASALS
                     ILEGIEQYLESKGIESLEDLIGRVK"
     gene            2853..3446
                     /gene="pyrE"
                     /locus_tag="PKN31_07180"
     CDS             2853..3446
                     /gene="pyrE"
                     /locus_tag="PKN31_07180"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOA99741.1"
                     /translation="MEKEEILEIFRETGAFLEGHFLLTSGLHSPVYIEKFKVLQFPRY
                     VEILAGEIAHHFSGEKIDVVAGPAVGGIILAYEVARQLGVRMAFTEREEGKMVFRRDF
                     QLKKGDRVLIVEDVVTTGASVEEVIKAVEGTEAQIVGIGVLVNRGTKKIPFPYPFYPL
                     LEIEAQTYSPEECPLCQKKITLQKRGSRYLSQEESRA"
     gene            3439..>4417
                     /locus_tag="PKN31_07185"
     CDS             3439..>4417
                     /locus_tag="PKN31_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOA99742.1"
                     /translation="MPDWKALLDNEFDLFEPINLAQNLVRIPSVSGEGGEVELARFIA
                     NYFIDRQIKVEWQEVEEGRANLIAEIEGILGEGPNLLFNGHLDTVPLGSGWTYPPLGG
                     EIIENRLYGRGACDMKGALASMMYAAYIVSLFAPSLYGKLKLVFVVDEEGSNLGVKHW
                     IKSYREREEVIDFALVGEPTGLNISLGHRGVAAYQVKVRGKSCHMGIAERGVNAIYLA
                     SQVIQSIEKKNQELKKFADPDLGNPTLHVGRIQGGTFPNVVPDTCGFEVDVRTVPDFP
                     LGRMEETLQDILDSVQQEKELKFNYQIDQSIPYLPPVKISRDLEGVKILS"
BASE COUNT         1226 a          699 c         1186 g         1306 t
ORIGIN      
        1 tcaactgggt ggagctgtgg gaaaaactga gttataatcc agcactcttt ttagggctcg
       61 acaacaaagg gattctggaa gaaggaagag atgctgacat caccattgtg gatccaggaa
      121 ccagaaaaag agtcgaagtt tctaattttg aatcacaagg gaaaaattgt cctttcgaga
      181 attggtcttt accggggtgg ccggtttaca ctatagtaga tggtagaata gtaatggcag
      241 aagggagcat cgtagaaagt gaattttgct gaccgggtga taaaaaagac gcaggagaag
      301 ggacctttgg ctgtaggcct agatcctcac ttccagcttc tacctcctta tctgtgggag
      361 aaatgggtag aaaaagaggg aaaaacatgg gaagcacttt ctcattgtgt tttagagttt
      421 aattctctta ttttgcaggc tctttctcca gtagtgggat ttgcaaaaat tcaagtagct
      481 ttttatgaag ctttgggagt gccgggaatg gcggttttga aaaagaccat tgaggaagcc
      541 cagaaaaagg attttatagt aattctggat gggaagagga atgacatcgc cagcactgct
      601 actttttatg ctcaaggcta tctttctcaa ttagaggtag gaagagatag ttgtttgccc
      661 tctttctggg atgttgatgc tataactgtt aatccctatt taggtgaaga cagcttgcag
      721 cccttttttg aggaagctaa aaaagaagac aaaggagtgt ttgtcctgac tcgtaccact
      781 aatccttctg caccctttat tcaggatgtg ggagaagaaa aagtcttcat tcgcgtggcc
      841 cgattagtgg agaagtgggg agaaggagta aggggagaga gtggatttag ctctttagga
      901 atagtggtag gagccactta ttctgaagac atgaggtttt tacgggaaga gttcccttct
      961 cttttatttc ttgtgcctgg ggtgggagct cagaggggag atttttcttc tcttcgtttt
     1021 gcttttcgag aagatggaga gggggcgctt gtaactattt ctcgagatat aattttctct
     1081 tatctaaaag agaagagcaa tgaaggttgg ggatttgaag aaaaagcaag ggatagggct
     1141 atattttacc aggaaaagtt gagagaaatt ttaaaatgag tgaagccgta atagaagaaa
     1201 agaagtgctg gggagagcga tttgtccagt tgaggttgaa agaagaggaa atagcccgct
     1261 gtgctcgacc agggcagttt gtcatggtaa gggtacagga ggggtttgac cctttattgc
     1321 gtcgtcccct ggcagttatg aaagtggaag aaagtagttt ctaccttttc tgggaaatag
     1381 taggcagggg gacaaaaatt ctttcttctt tgaatgtggg aagtaaatta gaggtattag
     1441 ggcctctggg taggggattc tctctttttc cctctcaggc tttgattgtt gcaggtggaa
     1501 gaggaataac tcctctttac tttttggcct cccattttaa ggaaaataaa attcctttta
     1561 cttttttcct gggggtgaaa gataaggagg agctgtttat ggttgatttt ttgcaagatt
     1621 ttcctggtgc ttatttttgg agttgcgagg agccggtaga gggaggtttt tgcgggacgg
     1681 tgctttctct ttttcatacc tggcttgaag ataaatcttt ttctcctgga actggagttt
     1741 atgcctgcgg tccagaggga atgttgaaaa atctttatag agaaggaaaa acacagaatt
     1801 tccccttaga ggtttcttta gagactttta tgggttgtgg ttttggggca tgtatgagtt
     1861 gtgcagtaga aaagagcaat ggtaagggtt attttcatgt ctgtaaagat ggtcccgtgt
     1921 ttttagctca ggaattgaaa tggtgaatct ggcagtaagt ctgggaaaat taaatttaaa
     1981 aaatccagtg attttggcct ccggtacctg cggctatggg agagaaattt cctcctatct
     2041 ggatttaaat aaagtggggg caataacagt gaagggttta agttattacc ctttttccgg
     2101 gaatcctcct cctcgattac gagaagtata cgctggagtc ctcaattcca ttggtttaga
     2161 aaataaaggt gtttatgact ttattcagga agatcttccc tttctttctt cttttgatac
     2221 tcgccttttg gttaacatct ggggagaaac agcaaacgag tttgtgcagg tggcacaaga
     2281 gttagacagg tttgagagag tggatgcact ggagcttaat gtttcctgtc ctaatatgga
     2341 aagaggagga gcagtttttt gttctgactt ggaaattcta aaatctatag taggcggagt
     2401 gagagatgcg acccgaaaac ctctgattgt aaaattgggt cctgaagtaa aggaatgggg
     2461 gaaaactctg gatattctag agaatgaggg ggcggatatc ctaagtctta ctaattcttt
     2521 tcctgctctt tttgtagatg tgggagaaat ggattttttc tttaagcgaa aatttgctgg
     2581 actctctggt ccagctatta aacctctggc tctgaaaatg gtttatgagg tggtaaacta
     2641 tagctctctt ccggttattg gaatgggagg aattgttagt gctgatgatg ctctggagtt
     2701 tctcctggta ggagcgaaag cggtggctct gggagtggcc aattttgtgg atcctgcttc
     2761 agctctgtct attttggagg gaatcgaaca gtacttagaa agcaaaggaa tagaaagttt
     2821 agaagactta atcgggaggg tgaaatgata caatggagaa agaagaaata ttagaaattt
     2881 ttcgcgagac gggagctttt ctggaggggc attttcttct tacctctggt ttgcacagcc
     2941 ctgtttatat agagaaattt aaggttttgc aatttcctcg atatgtggag attctggccg
     3001 gggaaatagc tcaccatttt tcgggagaaa aaatagacgt ggtagcagga ccagcggtag
     3061 gggggataat tttagcttat gaagtagccc gtcagttagg agtaagaatg gcttttactg
     3121 agcgagaaga gggaaagatg gttttccgtc gtgattttca gttgaagaaa ggagaccggg
     3181 ttttaattgt ggaggatgtg gtaaccactg gagcctcagt agaggaagta ataaaggcgg
     3241 tggagggaac cgaagcccag attgtgggaa taggagtttt agtgaatcgc ggcacaaaga
     3301 aaataccttt cccttaccct ttttatcctc ttttggagat agaagcacaa acttattctc
     3361 cggaagagtg tcctctgtgt cagaaaaaaa ttaccttgca aaagaggggt agccgttacc
     3421 tctcacagga ggaaagtcgt gcctgattgg aaagctttgt tagataatga gtttgacctt
     3481 ttcgagccta tcaatctggc tcaaaatttg gtgcgtattc ccagtgtctc gggggaagga
     3541 ggcgaggtag agcttgctcg ttttattgcc aattacttca tcgaccgcca gattaaggta
     3601 gaatggcaag aagtggaaga gggaagagca aacctgattg ctgaaataga aggaatttta
     3661 ggagaaggtc ctaacctcct ttttaatggt catcttgata ccgttcctct gggtagtggc
     3721 tggacttatc ctcctctggg aggagaaatt atagaaaatc ggctttatgg gcggggagcc
     3781 tgtgatatga agggagcctt agcttctatg atgtatgctg cttatatagt ttctctcttt
     3841 gctccctccc tctatggtaa gctaaaatta gtttttgtgg tggatgaaga aggaagcaat
     3901 ttgggggtaa agcactggat aaagtcttat agagagaggg aagaggtgat agactttgct
     3961 ctggtgggtg aacctaccgg tctcaatatc agtttaggcc atcgaggggt agcggcctac
     4021 caggtaaaag taaggggaaa atcatgtcat atgggaatag ccgagcgagg agtaaacgct
     4081 atttatcttg cttctcaggt gatacaatct atagagaaaa aaaatcaaga attgaaaaaa
     4141 tttgccgatc ctgatctggg gaatcccact ttacacgtag gtagaattca aggagggaca
     4201 tttcctaatg tagttcctga tacttgcggt tttgaggtag acgtacgcac cgttcctgat
     4261 ttccctctgg ggagaatgga agaaactctg caggatattt tggacagtgt tcaacaagaa
     4321 aaagagctga aattcaacta ccagattgac caatctattc cttatcttcc tccggtaaaa
     4381 attagtagag acttagaagg agttaaaatt ctctctc
//
LOCUS       DAOPSS010000133         4406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_16019_length_4406_cov_7.385199, whole genome shotgun sequence.
ACCESSION   DAOPSS010000133 DAOPSS010000000
VERSION     DAOPSS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16019_length_4406_cov_7.385199"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..618
                     /locus_tag="PKN31_07190"
     CDS             184..618
                     /locus_tag="PKN31_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOA99743.1"
                     /translation="MVKAIGSRRATSPPKTYQPLEVEKPWGRFIQYTLNTPTTVKILE
                     INPGEVLSLQSHNYREELWIPLTDGVMAEIDGELYFPEELEPVFVPKHSKHRLSAGDK
                     KVRVLEISFGYFDEEDIVRYEDKYGRTKDELIEALEQKASNW"
     gene            618..1823
                     /locus_tag="PKN31_07195"
     CDS             618..1823
                     /locus_tag="PKN31_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOA99744.1"
                     /translation="MLSSLRIGFLSTYPPTPCGIATFTEDLVQAIPSSSFSPEIIAVS
                     QDDETISYPEEVKFIIQKEKKEDYWQAAEYANKELEAVSIQHEYGIFGGEDGEMVVEF
                     LDRLRKPAITTLHTVLSQPSPGQVAVLQKIIQRSEKVVVMNSLAIPILEDKYQAPSQK
                     LVVIHHGAPSSYSYEKEGQKSALGLSDRIVLSTFGLISRGKGIEYVIQALPEVVHKFP
                     QVVYLIIGATHPRVKAREGESYRNFLEQLVKELNLEEHVIFVNRYLSKEELVKYLVAT
                     DIYLTPYLNSQQIVSGTLAYAMRFGKVIISTPYLYAQELIGKDRGILVKFADSQSIKD
                     ALFRVLENPAYFRQIEKNAQKFGAKLKWTEVGRDYARLFEEIINQETVSFSERNQPDF
                     SSKSLLVED"
     gene            1835..2932
                     /locus_tag="PKN31_07200"
     CDS             1835..2932
                     /locus_tag="PKN31_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99745.1"
                     /translation="MVRDKIDMHILQEKAVLSSIKLDHLASLSDHFGLLQHADYKLPK
                     FEFGYTTDDNCRGLLVVSKFYSISPDPLAKKLAQRYLAFLRWVQKENGQFHNEVSIDR
                     RFLDVVGSEDCQCRTFWALSATLNTAIPVDFKLVIREMLEKFLPTLFSYSSPRPVAFL
                     IFGLKELSRQREITFPNLDIPSAVRTLAGRLVEQYERNASPQWAWFEDIMTWGNALLP
                     GSLFVSYQLLGDKKLLEIARESFDFMSDHVFDHQLIFQPVGNRGWFPRGGEKAQFDQQ
                     PIEAMWMTITGEEAFEATAQPHYRCKARSAIEWFWGRNCHCLPLYDPEDGSCADGLTP
                     DGLNPNRGVESTLSALHSLLLAKEKGILNNN"
     gene            complement(2940..3458)
                     /locus_tag="PKN31_07205"
     CDS             complement(2940..3458)
                     /locus_tag="PKN31_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HOA99746.1"
                     /translation="MKEFPKHLLITGRPGVGKTTLIKRLIPALPPAGGFYTEEIRSCG
                     ERVGFKIVTLAGEEGILAQKGLNSPFRVGSYGVNIEDLEKVGVKAINEALASCEVIVI
                     DEIGKMELCSPEFEKAVREAVNSEKRVLATIPVFPLPFLEGIKSRPDVAIFQLFYNNR
                     EEVYQKIKREFH"
     gene            3662..3844
                     /locus_tag="PKN31_07210"
     CDS             3662..3844
                     /locus_tag="PKN31_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOA99747.1"
                     /translation="MALRIDEEVCSGCELCVQICPDVFEMNSDGICRVKNGADESLDC
                     VQEAIDSCPTAAIIQE"
     gene            3913..3989
                     /locus_tag="PKN31_07215"
     tRNA            3913..3989
                     /locus_tag="PKN31_07215"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3948..3950,aa:Glu,seq:ttc)
     gene            4083..>4406
                     /locus_tag="PKN31_07220"
     CDS             4083..>4406
                     /locus_tag="PKN31_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOA99748.1"
                     /translation="MGKVLKLVQPKTWEEYFEEFLLQKQIEGLRPRTLEDYRYHINHF
                     FNRANLEDWDTIQKRVKEYFTENIAPATFNTRQKTLKAFFRYLVEQGAIPANPITFKK
                     IKEEGK"
BASE COUNT         1295 a          839 c          919 g         1353 t
ORIGIN      
        1 aaagtgacat tttcacagaa acatttaagg gtgacattat cacagaataa ctacactgct
       61 tttcttgtca atttgacatt aattctgttt tcacctacaa taagtacacg acagttaaaa
      121 aagttaatat tgattttctt cgctaattta gtgaggaacc aaaactttga ggaggtgatt
      181 attatggtca aagctattgg ttcccggcgc gcaacttccc ctcctaaaac ctaccagcct
      241 ttagaagtcg aaaaaccgtg gggcaggttt attcagtaca ctttgaatac tcccaccaca
      301 gtgaaaatac tggaaataaa cccgggagag gttttaagcc tccaatccca taattataga
      361 gaggaattgt ggatacctct tactgatgga gtaatggcag aaatagacgg agagttgtat
      421 tttcctgaag agttagagcc ggtgtttgtt cctaaacatt ctaaacaccg tctcagtgcc
      481 ggagataaga aagtcagagt tttggaaatc tcttttggtt attttgatga ggaagatata
      541 gtccggtatg aagataaata cgggcgaact aaggacgagt taatcgaagc cctggagcag
      601 aaagcgagca attggtaatg cttagctccc tgcgaatagg gtttttatcc acttatcccc
      661 ccactccctg tgggattgct acctttactg aagatttggt tcaagcaata ccttcttctt
      721 ctttttctcc agaaattata gctgtatctc aggatgacga gactatttct tatcctgaag
      781 aagttaagtt tataattcag aaggagaaaa aagaagatta ttggcaagca gcggaatatg
      841 ctaacaaaga attggaagca gtaagtattc agcacgaata tggtatcttt ggaggagaag
      901 atggagagat ggtggtggag ttcttagaca ggctgagaaa accagctatt actactttac
      961 atactgttct ttctcagcct tctcctggtc aggttgcagt tctccagaaa ataatccaga
     1021 gatcagaaaa agtagtggta atgaatagtt tagctattcc cattttagag gataaatatc
     1081 aggcaccttc ccaaaaattg gtggttatcc atcatggtgc tccctccagt tattcctatg
     1141 agaaggaagg gcagaagtct gccctgggac tcagtgatag aattgttttg tccacctttg
     1201 gtttgattag ccggggaaag gggattgaat atgtcattca agcgctacct gaggtagtac
     1261 ataagtttcc tcaggtagtg tatctaataa taggagccac tcatccccgg gttaaagcga
     1321 gagagggaga gtcatacagg aattttttag agcaattggt aaaagagctg aatttagaag
     1381 aacacgttat ttttgtgaac cgttatctga gtaaagaaga attggttaag tatctggtgg
     1441 ctaccgacat ttaccttact ccttatttaa attctcagca aattgttagt ggtaccttag
     1501 cttacgctat gcggtttgga aaagttatca tttccactcc ttacttatat gctcaagagc
     1561 tgatcggaaa agatagagga atattggtaa aatttgctga ttctcaaagc attaaagatg
     1621 ctcttttcag ggttttagaa aatcctgctt attttcgcca aatagaaaaa aacgctcaaa
     1681 agtttggtgc taaattaaaa tggacggaag tgggaagaga ttatgcccga ttatttgaag
     1741 aaattattaa tcaagaaact gtttctttct cggaaagaaa tcagcctgat ttctcaagta
     1801 aatcactatt agtcgaagat tagctaaatt ttgaatggtg agagataaga tagatatgca
     1861 tattttacag gaaaaagcag ttctttcctc aatcaagctg gatcatttag cttccctcag
     1921 tgaccacttt ggtcttttgc aacatgctga ttataaactt cccaaatttg agtttggata
     1981 tactactgac gataattgtc gaggactctt agtggtttct aaattttact ctatttcccc
     2041 tgatccgctg gcgaaaaaat tggctcagcg gtatctggct ttccttcgtt gggttcaaaa
     2101 ggaaaatggt cagtttcata atgaagtttc tatcgaccgg cgtttcctgg atgtagttgg
     2161 ttcagaagat tgtcaatgtc gaaccttctg ggctttatca gctactttga ataccgctat
     2221 tcctgtagac ttcaaattag ttataaggga gatgctggaa aaatttcttc ctactctatt
     2281 ttcttactct tcgcctcgcc cggtagcttt tttaattttt gggttgaaag agctttcccg
     2341 gcaaagagaa atcactttcc ccaatttaga tataccttct gctgttcgta ctctggcagg
     2401 aagattagtt gaacaatatg aaagaaacgc ttctcctcaa tgggcctggt ttgaagatat
     2461 tatgacctgg ggcaatgctc ttcttcctgg ttctcttttt gtttcttatc aacttttggg
     2521 agataaaaag ctattagaaa tagccaggga gagtttcgat tttatgagcg atcatgtatt
     2581 tgaccatcag cttatatttc agccagtggg aaatagaggg tggtttcctc ggggagggga
     2641 aaaagcccaa tttgatcagc aacctattga ggccatgtgg atgactatta ctggagaaga
     2701 ggcgtttgag gcaactgctc aaccccacta tcggtgtaaa gctcggtctg ccattgagtg
     2761 gttttgggga agaaactgcc attgtcttcc tctttatgac cctgaggacg gatcctgtgc
     2821 tgatggatta accccggatg ggcttaatcc caatcggggg gtagaaagta cgctttctgc
     2881 tttacacagc cttcttttgg ctaaagaaaa gggaatctta aacaacaact aattcaaatt
     2941 cagtgaaatt ctctttttat tttttgataa acttcttctc gattattgta gaaaagctga
     3001 aaaattgcca cgtcagggcg gcttttaata ccttccagaa aagggagggg aaaaacgggg
     3061 atagtagcta aaactctttt ttcactattc actgcctctc tcactgcttt ctcaaattca
     3121 ggagagcaaa gctccatctt tccaatttca tcgatgacta ttacttcaca agaagcaagg
     3181 gcctcattaa ttgcttttac ccccactttt tctaaatctt caatgtttac tccatagctg
     3241 cctactctga agggagaatt tagtcctttt tgagctaaaa ttccttcttc tccggccaga
     3301 gtaacaatct taaagcccac tcgttctccg caggatctta tctcctcagt ataaaaacca
     3361 ccagccgggg gtaaggcagg aatgaggcgc tttattaaag tggtttttcc tactccaggt
     3421 cttccggtta ttagaaggtg cttgggaaat tctttcatca aatagacaac ttcccttctt
     3481 tatctcgata atgacatttt tgtatatcaa cctaactcat ctgaaacgct gaccctatta
     3541 tctcctaaat attttaaata ttaaagaagt ttttataatt aattcaccac ttttggataa
     3601 atatgctata atgttttccg gtttgcaaaa attacacttt taccagagga ggatatagtt
     3661 tatggcgcta cgaatagatg aagaagtgtg ttcaggatgt gagctgtgtg tccagatttg
     3721 tcccgatgtt tttgagatga attccgacgg tatatgcaga gtaaaaaatg gagctgatga
     3781 gagcctggat tgtgtacaag aggctatcga ctcctgtcct actgctgcta tcattcagga
     3841 ataggaaaag ttttttttga ccccttgcct tatataaagg ggttgtataa aataggtaac
     3901 caaaataaaa gcgcccccat cgtctagccc ggtaaggaca ccgccctttc aaggcggcgg
     3961 cagcggttca aatccgcttg ggggcgccat tatttttgta gagccacttt tagcacttcc
     4021 tccttctctt ttttcctttt cttaaccccg aagggcgttg tccataaaaa gggggtatta
     4081 gaatgggaaa agttttaaag ttggttcaac ctaaaacgtg ggaagagtat tttgaggagt
     4141 ttttattaca aaagcaaatt gagggtctaa gaccacgcac cctcgaagat taccgctacc
     4201 acataaacca tttctttaat agggcaaacc ttgaggattg ggacactatt caaaagagag
     4261 taaaagagta ttttactgaa aacattgccc ccgctacctt taacactcga caaaaaactc
     4321 ttaaagcttt ctttcgctac cttgtagagc aaggagctat tccagcaaat cctataacct
     4381 tcaaaaagat aaaagaagag gggaaa
//
LOCUS       DAOPSS010000134         4329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_16359_length_4329_cov_8.794572, whole genome shotgun sequence.
ACCESSION   DAOPSS010000134 DAOPSS010000000
VERSION     DAOPSS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16359_length_4329_cov_8.794572"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            843..1592
                     /locus_tag="PKN31_07225"
     CDS             843..1592
                     /locus_tag="PKN31_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOA99749.1"
                     /translation="MSPILLGTVASLLAGLATGVGALPVLFGRGQKISHRFRDELLGM
                     AAGIMLAATAFSLLVPAIETGGAWIAALGVAIGGVLLDVFDKIIPHEHFERGREGLGP
                     ELKQIWLFVLAITLHNFPEGLAVGVSFGSGNISDGITIALAIGLQNIPEGLAVAVSLL
                     GVGYNHRQAFTYALLTGLVEPIGGFLGISLVTIMYSFLPLGMALAAGAMLFVISDEII
                     PETHHSPHARSSTYALLLGFIVMMILDTVLG"
     gene            1690..4290
                     /locus_tag="PKN31_07230"
     CDS             1690..4290
                     /locus_tag="PKN31_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOA99750.1"
                     /translation="MKIRTKIPLGFIVLALLAAVVGGFAIYAFQEIGTSLEEVRSSTP
                     LLLATSRIKDFLSEKDSLVSSYLLEEDTATLDQIKGELELLDNKIMTYIEALRLGTES
                     EEFEASSYYSIWEKEEFPYSLPLLEEGTPLYNSLQNLKGFEVGHQSKVNSVTTLWREF
                     LIGRQQRDEAALAMDEPAQVVVNFIQSVAQAVEKFTQPVEEINSLISRWLATGDPAGT
                     IAVSINSYFESFSNDIDTSSMISDQTKKDIKDKLALYRQNWEKLQNNFPIATPEERDN
                     LYMECYRSYSSLKRSIENLKMDKWMDKLNTINQERKNYLLLSDPEAKETARQKVETNL
                     EQMKKFMEEDFLKTYGSVIAETVINDRWKPFVELWNEIVIKDNYLKFAEEQTKKAITE
                     VRRTGQEVLAAMNDMNQEVLDQFDTTVARVSELQNRLSQLLYLIVGIIVILAIILGIV
                     ISRSIVSPINRSVSFAQVLEKGDLTQEISLRRRDELGILFNSLNQASRSLRTFLKEVA
                     EGAETIISSMENLQHASREIAQTGDQIGQTISQVARGSEEQSQNLTEISQNMEDLVNL
                     VKGMAEQLKKQSNKAYTTLEAVDQVMESIQLTGGNLEKVQESVTQAFSLSEEGQKTLK
                     EVVEAMESISNSVLSVGEIVKTLGYSSREIGSITDLITGIADETNLLALNAAIEAARA
                     GDAGRGFAVVADEVRKLAEESAHAAQRIAGLIGDIQKETNKAVNSMEESTKRVESGTQ
                     AVDRARRAFQDIYQANQVVTEETNSISSSFVRVEEISQKIVQLVREMSEISRQNEAET
                     QEVVEKAEEVFTRLSNVASISEENAAAAEEVAASAEEQSAALQEMDKAIQETMDMGRA
                     LQEDLRHFTI"
BASE COUNT         1357 a          821 c          977 g         1174 t
ORIGIN      
        1 cctttttgaa gaggggaagg ccttaaagaa agaatgggga gtgtctgatg aatatattcc
       61 ctatgctgca gcggtgtttg gataccaagc taaagagccc atgtatcgcc ctcctcgtaa
      121 ggagggagtg gtgaaaatca tcagctaacc gggtggtgta attttatgtc tgctacagtt
      181 tccgagaaag agagataccg aaatgtgggt atttattttc tagaggatat aacctcgccg
      241 gaatttagtc accagagtta cttcatttaa acatagcatt tggattaatt tacaacaaag
      301 aatataaaga aatcgggcca gagaaaaact ccccgggggc aacacctgca gattttggtc
      361 ccgagaagca gcatacaatg tcagaagagg ggtgttaccc ctgaacgtat ttatcatctc
      421 ttttcgtcac cccggtgact ttcgagccgg ggtctatgtt attgaggaca gaaactatgg
      481 atgcctgatt aaagatttca ggcatggcga aagagggatg tcacccttga atagttctat
      541 cgactacaag gaggcgctgc ccccttataa acccgtaaga gcacaaaaga taggaaaagc
      601 attaaaataa ttagctggag aaaaaacatt ccaacattgt tatctcagtt ttttaaacct
      661 tttttacttt ttcttatgcg gcaggctacc aaacgaggtt cataacaacc cgcatgtgaa
      721 gtgaagtatt taacgcagag agagaaggca tgatttttct gattaatata atcggtaaac
      781 attaaagtag cttgtcatat ccgggaaatt aagagagaat aaaataaaaa agggtggtgc
      841 ttgtgagtcc tattctttta ggaactgtag ctagtctttt agccgggttg gctaccggtg
      901 tgggagcatt accggttctt tttggaagag gtcaaaaaat ttcccaccga tttcgagatg
      961 aactattagg tatggcagca ggaataatgc ttgccgctac tgctttcagt cttttagttc
     1021 cggcgattga gacgggagga gcatggatag ctgcactggg ggtagccata ggaggagttc
     1081 tgctcgatgt ttttgataaa ataatacctc acgagcactt tgagagggga agagaaggac
     1141 tggggccaga gctaaagcag atctggttat ttgtcctggc tatcacttta cataatttcc
     1201 cggaaggctt agcagtagga gtgagctttg gcagcggaaa tatatctgat ggtattacga
     1261 tagctctggc cattggtcta caaaatatcc ccgaaggact ggcagtagct gtttcccttt
     1321 taggagtggg gtataatcat cgtcaggctt ttacttatgc tttgctcacc gggttggtgg
     1381 agcctatcgg aggattttta ggaatttctc tcgttaccat tatgtattct tttcttcctc
     1441 tggggatggc tcttgccgca ggagcaatgc tctttgtgat tagtgatgag ataatacccg
     1501 aaactcacca ctctccccat gctcgctcat ctacttatgc ccttctttta gggtttatag
     1561 ttatgatgat tctggacact gttttgggat aaacttctaa tcagactacc aaacatcata
     1621 aagtttttgg caaatttgcc gattaactaa tacagaaagt atataaaaat aaaattggga
     1681 gggataaagt tgaaaatccg aaccaagatt cccttgggat ttattgtcct agcactgctg
     1741 gcagcagtag taggtggctt tgccatttat gcttttcaag aaataggcac gtctttagag
     1801 gaggtgagaa gtagcactcc tttactttta gccacttcca gaattaaaga cttcctttca
     1861 gaaaaagatt ctctggtatc ttcctatctt ttagaagaag acaccgccac tctcgatcaa
     1921 attaagggag aattagagtt gttagataat aaaatcatga cctatattga agctctgagg
     1981 ctgggaacag aatcagagga atttgaagcc tcttcttact attctatctg ggaaaaagag
     2041 gaattcccct actccctacc tcttttagaa gaaggaactc ctctttacaa tagcttgcag
     2101 aatttaaaag gttttgaggt tggccatcaa agtaaagtga attcggtaac tacgttgtgg
     2161 cgcgaattcc ttattggtcg ccagcaaaga gacgaagctg ctttagctat ggatgaacca
     2221 gcccaggtgg tggttaattt tattcagtcg gtggcacaag cagtggaaaa gttcactcag
     2281 ccagtagagg aaatcaactc tcttatctcc cgctggttag ctaccggtga tcctgctgga
     2341 acaatagctg tttctattaa ctcttatttc gaatcttttt ccaatgacat cgatacttcc
     2401 tctatgattt ctgaccagac caaaaaagat ataaaagata aattggctct ttatcgtcaa
     2461 aattgggaga aactacaaaa caacttccct attgccaccc ctgaagagcg agacaattta
     2521 tatatggaat gttaccggtc ttatagctct cttaagagaa gtatagaaaa tttgaaaatg
     2581 gataagtgga tggataagct aaacaccata aaccaggaaa gaaaaaacta tcttttattg
     2641 tctgaccccg aagccaaaga aaccgcccgc caaaaagtgg aaaccaatct agaacagatg
     2701 aaaaaattta tggaagaaga ttttctaaag acttacgggt cggttattgc agaaacagta
     2761 attaacgacc gctggaaacc ctttgttgaa ctgtggaatg aaatagttat taaagataat
     2821 tatttaaagt ttgctgaaga gcaaactaaa aaagctatta ctgaagtaag aagaacgggc
     2881 caagaagttt tggcggcaat gaacgatatg aatcaggaag tgcttgatca atttgatact
     2941 actgttgctc gcgtcagtga gcttcaaaac cgattatctc agcttctcta tttaatagtg
     3001 ggtattatag taattctggc cattatttta ggcatagtta tctcaagaag catcgtctcc
     3061 cccataaatc gcagtgtttc ttttgcccag gttttagaaa aaggagattt aactcaggaa
     3121 atctctctcc gccgccggga tgaactggga attctcttta actccttaaa ccaggcttcc
     3181 cggtccttac gaacgttcct aaaagaagta gccgagggag cagaaacaat catatcctct
     3241 atggaaaacc tccagcatgc ttctcgagaa attgcccaaa ccggagatca gattggccaa
     3301 actatatctc aagtagcccg aggttcagag gaacaaagcc agaatctaac tgaaatctct
     3361 caaaatatgg aagaccttgt aaacctggtc aagggaatgg ctgagcagct gaaaaagcaa
     3421 tcaaataaag cttataccac tttagaagca gtagaccagg ttatggaaag cattcagctc
     3481 accgggggca acttagaaaa agttcaagaa tctgttactc aggctttcag cctctctgaa
     3541 gaaggacaaa aaactttaaa ggaagtagta gaagccatgg aaagcatcag caattcggtc
     3601 ctttccgtag gagaaatagt aaaaacatta gggtacagtt ctcgagaaat tggaagtatt
     3661 actgacctca taactggtat cgctgatgaa accaatcttt tggccttaaa tgcagcaatc
     3721 gaggctgctc gagccggaga tgcaggaaga ggatttgctg tggtggctga tgaagtgaga
     3781 aaattagctg aagaatcagc ccatgctgct caacgaattg ctggtttaat tggagacatt
     3841 caaaaagaaa ccaataaagc ggtaaattct atggaggaaa gtaccaagag agtagagagc
     3901 ggaactcaag cagtggatag agctcgacgg gcttttcaag atatttatca ggctaatcag
     3961 gtagtaactg aggaaaccaa ttctatctct tcttctttcg tacgagtaga agagatatct
     4021 caaaaaatcg tccagttggt tcgagaaatg tcagaaattt cccgtcaaaa tgaagctgaa
     4081 actcaagaag tagttgaaaa ggctgaagag gtatttacca gactcagcaa cgtcgcctcc
     4141 atttctgaag aaaatgctgc tgcagccgaa gaggtagctg cctcagccga agagcagagt
     4201 gccgcactac aagagatgga taaggcaatc caggaaacta tggatatggg aagggctcta
     4261 caggaggacc tccggcattt tactatttaa atcccgatta aggaataaag atcattgata
     4321 aagtgtata
//
LOCUS       DAOPSS010000135         4327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_16368_length_4327_cov_7.340356, whole genome shotgun sequence.
ACCESSION   DAOPSS010000135 DAOPSS010000000
VERSION     DAOPSS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16368_length_4327_cov_7.340356"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKN31_07235"
     CDS             complement(<1..583)
                     /locus_tag="PKN31_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA99751.1"
                     /translation="MYWPFFPRFKSLELDFVQWVIVFLCFYGIYQAIVSFRGFWPAKP
                     LPPASSLRRFAILVPAHNEEKVIGSLLDSLNKQSYPREFFDVYVACDACNDATPEIAK
                     EYGGIALLRNDPLHPGKTQNIRWALNNIPLDEYEAVVIFDADNLANPHFLSQMNDYLE
                     AHPGAEAVQGYLDTKNFSDSWLTRVYALAYWYTN"
     gene            complement(616..1146)
                     /locus_tag="PKN31_07240"
     CDS             complement(616..1146)
                     /locus_tag="PKN31_07240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA99752.1"
                     /translation="MIWGIGLFIIAVALLFFFWFFPVFFNGIPWQPTDMKRVRRMLEM
                     AEVKPDEVVYDLGCGDGRIIICAAREFGARAVGVELNPWLYFLALVKVWVRGLSRRVK
                     VIFGNLHQVDLKEADVVTIFLYSHVNDLLQEKFQRELKPGARIVSYVWVLKNWKPVAV
                     DSEHDLYLYVKNGAGW"
     gene            complement(1143..1994)
                     /gene="lipA"
                     /locus_tag="PKN31_07245"
     CDS             complement(1143..1994)
                     /gene="lipA"
                     /locus_tag="PKN31_07245"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOA99753.1"
                     /translation="MNSLPSWIKKRISLGEGEETQKLLQSLSLHTVCESARCPNRGEC
                     FARKVATFLIMGSICTRRCRFCAVEKGIPQPLDFEEPRRVGEASRQLGLKHVVITSVT
                     RDDLPDGGAYHFVATIREIRKVLPFSTIEVLTPDFQGREEDLQVVVKEQPEVFNHNVE
                     TVPRLYPVVRPLASYERSLTILKRVKEISPSIITKSGLMVGLGEGKEEVLEVLEDLRG
                     VGCDVVTIGQYLRPSPRHLEVKEYVPPQVFTWYEDIAYSLGFRGVASGPLVRSSYLAE
                     KFVGEQL"
     gene            complement(1991..2698)
                     /gene="lipB"
                     /locus_tag="PKN31_07250"
     CDS             complement(1991..2698)
                     /gene="lipB"
                     /locus_tag="PKN31_07250"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOA99754.1"
                     /translation="MEKLWFVRGGQIEYSFAHSLQLSLRDKVERENIPGFLLLMEHPP
                     VFTLGKGASRNHLLVDESVLEKEGIKLYEVERGGDITYHGPGQIVGYPIINLNFWRKD
                     VHLFLRSLEEAIIRFLRRFEVEAFRLPPYTGVWVRRETPQKIAAIGIAVKRWVTYHGF
                     ALNISTFLPHFSYIVPCGIIDKGVTSLEHVTGHFYSLEEREKMQEFLAQDLGEVLGFR
                     VKEISKVDLILERGLSL"
     gene            complement(2731..2824)
                     /locus_tag="PKN31_07255"
     tRNA            complement(2731..2824)
                     /locus_tag="PKN31_07255"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2788..2790),aa:Ser,seq:gct)
     gene            complement(3206..>4327)
                     /locus_tag="PKN31_07260"
     CDS             complement(3206..>4327)
                     /locus_tag="PKN31_07260"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HOA99755.1"
                     /translation="SKSKWLYFFIGVLAILIILFVSFAPRAHYTRAYIEENRGKVIVE
                     IPEVFELANIIIAIANHELTNNPYSVFQQGEYYERVLQHFLPFKNHPLISEIRREFSG
                     EDQGSKYYRFRDNSVCYVFKGDSIVNAGLYSVVFSPDLFKEYIDLVEDFARVSGFREF
                     YDANQSYYQEQIQKYKERVPVKKMWDWLENNFPQRYDCYKVIFSPLIQGMHHTERFET
                     KKFKEIVMFICGPDAFHVRLPEKIEEGLLSRAVFTEIDHNYVNPITKKYSDKVEKAFS
                     DIQKWNKQSRYWYTTPVITFNEYMTWAVFTLYAYDTYEKEDFEIINQFTVNQMVNSRG
                     FVLFEQFNNQLLELYINREEGEAIPDLYPEILEWVENYL"
BASE COUNT         1351 a          982 c          758 g         1236 t
ORIGIN      
        1 gattggtata ccagtaggct aaggcataaa ccctggtaag ccaggaatca gaaaagtttt
       61 tggtatccag atagccctga acagcttctg ccccgggatg ggcttctaaa taatcgttca
      121 tctgagagag aaaatgagga ttagctaaat tatcagcatc aaatataact actgcttcgt
      181 attcatcaag agggatattg ttcaatgccc agcgaatatt ctgagtcttt cccggatgga
      241 gaggatcgtt acgcagtaaa gctattccac catactcctt tgcaatttct ggggtagcat
      301 cgttacaggc atcacatgct acataaacat caaagaattc ccggggatag ctttgtttat
      361 tgagactatc cagcagagag ccgataactt tttcttcgtt atgagcagga accaaaatag
      421 caaagcgccg cagagaagaa gcggggggta agggctttgc tggccaaaaa ccacgaaaac
      481 tcacgatggc ctggtatatt ccgtagaagc aaagaaaaac aatgacccat tggacaaaat
      541 ccagctctaa cgacttaaag cgagggaaga acggccaata caatatatca ctcctttttt
      601 aatagttcaa aaaacctacc aaccagcacc atttttcacg tacaaataaa ggtcgtgttc
      661 actatccaca gctactggtt tccagttctt caaaacccaa acataggaaa caatacgagc
      721 tcctggtttt aattctcgct ggaatttttc ttgcaaaagg tcatttacgt gagaatataa
      781 aaatatggta accacgtctg cttcttttaa atctacttga tgcaaattac caaaaattac
      841 ttttacccgg cgggaaagcc ctcttaccca gactttcacc agagctaaaa aatagagcca
      901 cggattaagt tctactccca ctgcccgagc accaaattct cgagcagcac aaatgataat
      961 cctcccatcc ccgcagccta aatcataaac tacctcatca ggcttaactt cagccatctc
     1021 taacattctt ctcactcttt tcatatccgt aggttgccag ggaataccat taaagaaaac
     1081 cgggaaaaac cagaaaaaaa ataaaagagc tacagcgatt ataaatagcc ctatccccca
     1141 gatcataact gttcgcccac aaatttttct gctaaataag aactacgaac taaagggcca
     1201 ctggctactc cccgaaagcc taaagagtaa gctatatctt cataccaggt gaaaacctgg
     1261 ggaggaacgt attcttttac ctctaagtgc cggggagaag ggcgaagata ttgaccaata
     1321 gtaaccacat cacatcccac acctcttaag tcctctaaca cttctaacac ttcttccttt
     1381 ccctccccaa gacctaccat caaacccgat ttagtgataa ttgagggaga aatctctttt
     1441 accctcttaa ggatagttaa tgacctctca taagaagcca gaggtcgcac cacagggtaa
     1501 agacgaggta cagtttccac gttgtggtta aaaacttctg gttgctcttt tactaccact
     1561 tgcaaatctt cttctctgcc ttgaaaatca ggagtgagaa cttctattgt agaaaaaggg
     1621 agaactttgc gaatttctcg aatggtagcc acaaaatgat aggcacctcc atcaggaagg
     1681 tcatcccggg tgactgaggt gataaccacg tgcttcaatc ccagctgccg agatgcttcc
     1741 cccacccttc ttggttcttc aaaatccaga ggctgaggta ttcctttttc taccgcgcaa
     1801 aaacgacacc gtcgagtaca gatgcttccc atgattagaa aagtggctac ttttctcgca
     1861 aaacattctc ctcgattggg gcaacgggca ctctcacaaa cagtatgaag agaaagagat
     1921 tggagcaatt tttgtgtttc ttctccttct cccagggaga ttctcttttt tatccaggaa
     1981 ggtaaactat tcaaagactt aatccccttt ctaaaatcaa atcaacttta gatatttctt
     2041 tcactcgaaa tcctaaaacc tctcctaagt cttgagccag aaattcttgc atcttttctc
     2101 tttcttccag tgagtaaaaa tgaccagtaa catgctccag ggaagttact cctttatcaa
     2161 tgataccaca gggaacaatg taactaaaat ggggaagaaa agtggaaata ttaagggcaa
     2221 aaccatgata agtaacccat ctctttactg ctatacctat agcagcaatt ttctggggtg
     2281 tctctcgcct gacccatact ccggtgtaag gaggaagacg aaaggcttcc acttcaaacc
     2341 tcctcaaaaa ccggattata gcttcttcta aagaacgcaa aaaaagatga acatcttttc
     2401 tccaaaaatt aagatttatt ataggataac caacaatttg ccccggccca tggtaagtga
     2461 tgtctcctcc tctttctact tcatacaatt taataccctc tttctctaaa acactttcat
     2521 ctaccagaag gtgatttcga gaagctcctt ttcctagagt aaaaaccgga gggtgttcca
     2581 tcaaaagaag aaaaccgggg atattttctc tttctacttt atctcggaga gacaactgca
     2641 gactatgggc aaatgagtat tctatctgtc ctcctcggac aaaccagagt ttttccactt
     2701 atcctaccac gactttgcca gacataaact tggcggagag ggtgggattc gaacccacga
     2761 ggcgaggctt aactcgccta atcgcttagc aggcgactgc cttcagccac tcggccacct
     2821 ctccgctttt cgttagtata tcatattttt ttattttctc aacttctata attttaacaa
     2881 gttttgggta gccactttat gacactatca gctaacctat agttagctga aataacgccg
     2941 gagcactata tatccaggcg tgtgcgacag tttcttccct tctgtccccg caagcacaaa
     3001 cagcttacag agtagagcta cagtgaataa tactcctata gctcttagaa aacataaccc
     3061 agtagtattt aaattcctgg ctaccaactt aaggaataga aagtatgagg acaaccttta
     3121 aaggagaaaa ataggatatg cacaacttac aataactcct atgctaccat gagatatata
     3181 ataaggtttg tgataatatt cttcattaaa gataattttc aacccattcc aaaatttcgg
     3241 gatacaaatc aggtattgct tctccttctt cccgatttat atagagctct aacagttggt
     3301 tgttaaactg ttcaaaaagt acaaatcctc tggaattgac catttgattc actgtgaatt
     3361 ggtttattat ttcaaaatct tctttttcat aggtatcata ggcatacaaa gtgaaaactg
     3421 cccaggtcat gtactcattg aaagttataa caggtgttgt ataccagtac cgactctgtt
     3481 tattccattt ttgaatatca gaaaaagcct tctccacttt atctgagtac ttcttggtta
     3541 tgggattgac gtagttatga tctatttcag tgaagaccgc tcgagataaa agtccctctt
     3601 caatcttctc aggaagtctg acatgaaaag catctggtcc acagatgaac atcacaattt
     3661 ctttaaactt tttggtttca aaccgttctg tgtggtgcat gccttgaatc aggggtgaaa
     3721 agataacttt atagcaatca tatcgctgag gaaaattgtt ctccaaccag tcccacatct
     3781 tcttcaccgg gactctttct ttatattttt gaatttgctc ttgataatat gactgatttg
     3841 cgtcgtaaaa ctctctgaaa ccagaaactc gggcaaaatc ttccacaaga tcaatgtatt
     3901 ctttgaataa atcaggtgaa aatactacag aatacagtcc tgcgttgact attgaatctc
     3961 ctttgaaaac atagcaaaca ctattgtccc taaaacgata atacttagag ccttgatctt
     4021 ctcctgaaaa ttcccttctg atttcagaga ttaaaggatg attcttaaag ggaagaaaat
     4081 gctgtaaaac tctttcgtag tattcaccct gctggaaaac gctataggga ttattcgtca
     4141 gttcatgatt agcaatggct attatgatat ttgctaactc aaatacttca ggaatttcta
     4201 ctatcacttt acctctgttt tcctcaatgt aggctctcgt gtaatgtgcc cgaggagcga
     4261 agctaacaaa caatataatt aatatagcca atacaccaat aaagaaataa agccatttac
     4321 ttttact
//
LOCUS       DAOPSS010000136         4112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_17498_length_4112_cov_6.127434, whole genome shotgun sequence.
ACCESSION   DAOPSS010000136 DAOPSS010000000
VERSION     DAOPSS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17498_length_4112_cov_6.127434"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      372..546
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            654..1595
                     /locus_tag="PKN31_07265"
     CDS             654..1595
                     /locus_tag="PKN31_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOA99756.1"
                     /translation="MSKKSSTTRNRIALFFLGLWFLVFSSFPGAQAETISDDAGREVE
                     IPSPCERIVSLSPATTEIVFSLSLGEKLVGVTTYCNYPEEAKKKEKVGTINEISLEKV
                     VELQPDLVLASSLTPPNVVERLESLGITVFVLDPHNISEVIEGIEKVAIISGAKDKGK
                     ELKEAMEEEIREVRDKVSHLSPEERPTVFHLLWHDPLWTAGKDTFVNEFITIAGGQNA
                     FADLSGYVIVDLEELIRRNPDIITVVEDHGEEGSLSYQFLLSDSRLKVVNAIQNHKVF
                     PVDSDIVSRSSARVVDAVRIFASIIHPEIFGEYEYQS"
     gene            1579..2577
                     /locus_tag="PKN31_07270"
     CDS             1579..2577
                     /locus_tag="PKN31_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOA99757.1"
                     /translation="MNTRVKKGLTYLGFLALVLGSVFLAQGVGGGRYQLSHSLSLFFP
                     SDLSPSEKIILFEIRLPRIVLSFLVGGSLSLGGAVLQGIFQNPLVDPYVLGISSGAAL
                     GAVIAILSGVYLGFFTVPLFAFSMALFSAFLVFYLGKIGKRFPLEVLLLAGIGVGFFF
                     SALVSLGMFLAGEDLHQLFFWTMGGLWNSSWQEVKMILPFIVVGAPILFSFFRELNAF
                     LLGEKVAESIGVEVEKVKKQLLFVVSLLVASAVAVSGVIGFVGLVVPHLVRLLGARDH
                     RHLLPFSFLLGGGVLLWADTLARTILYPVELPVGIITSLLGAPFFIYLLRKRKKSL"
     gene            2574..3818
                     /locus_tag="PKN31_07275"
     CDS             2574..3818
                     /locus_tag="PKN31_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99758.1"
                     /translation="MKEYLQVENLSFSYSRRKVLDKLTFGVRRGELVGVIGPNGAGKS
                     TLLYLLAGLIVPESGKIVLGEKDTRFLSRRMWSQKVAMVFQEIPQNLELTSQEVIAMG
                     RFPHIGRWQREGDVDEEKVSWAMEITSTAVFSDRLFSELSGGEKQRVMIARALAQEPE
                     LLLLDEPTSHLDVLHQLEIMDILLELRAAGVAIVSVFHDLNLVSQFCDKALLLKEGRM
                     LSFGGVEEVMKAEEVEKLFGVEFLEATHPFSLRPFFMPLRRIKNETHRGKIHLICGGG
                     SGSPLMRELLEAGFSLSLGVVNQLDSDEELARKLGIPVVQEKPFSPFSPSTMEEAYRL
                     AQKADFIVIAPTYWGRGNLPNLDLAERLVLEGKAVLILEEALNPRLDYTEGAAQEKLK
                     KIIEEGGEVIKDLSAYFSSSSS"
BASE COUNT          981 a          911 c         1038 g         1182 t
ORIGIN      
        1 caagggagag gaaaaaagtt caggaatgag aacatcctct tctcttgttt aggttcctcg
       61 tagaggcatg ccgggtgaca aacaagaagg ggatttataa gggggaattc tttcccgctt
      121 atactgggaa atcttttctt atcagtagtc tttgcctttt aggctttatt tttaatcttt
      181 agtttttaat cttacgtcgt agactgtctg ttttctctcc tccagtagtt tttttgtttt
      241 ttcaagggat cataagggat tgcttcccct tataatcccc ctataaccat gaaaagattt
      301 gcaaaatacc gtagcttaag acacaaaagt tgctcaagaa gtttttcttc cttggtgttt
      361 gtgctataat ttcccgtgga atggtttcag gtggttttgc cttaataggg aagtccggtg
      421 aaaatccggc gcggtcccgc cactgtaacc ggggagcaag ttgccagagc cactgacgaa
      481 agtcgggaag gggcaacaag cgaagatccg ggagccagga gacctgcctg agattgtccc
      541 gaccactctt cgtggaaaga ggaggtggga aatttgctgg attccgcccc agacaagagg
      601 acgctccctc tcttcccccc tcctccacca gactaatata agaggaggaa attatgtcaa
      661 aaaaaagcag cacaacgagg aacagaatag cacttttctt tttgggactg tggtttttgg
      721 ttttttcttc ttttcccgga gcccaagcgg aaactattag tgatgacgca gggagagagg
      781 tggaaattcc ctccccctgc gagagaatcg tttctctttc tcccgctact acggagatag
      841 tcttctctct ttctcttgga gaaaagttgg tgggggttac tacctattgc aattatccgg
      901 aagaagcaaa aaagaaagaa aaagtaggaa ccatcaatga aatcagctta gaaaaagtgg
      961 tggagctgca gcccgatttg gttttagcca gctccttaac tcctcccaat gttgtggagc
     1021 ggttagagag cctggggatt accgtttttg tattagaccc tcacaatatc agcgaagtga
     1081 tagagggcat agaaaaggtg gcgataatat cgggagcaaa agacaaaggg aaagagctaa
     1141 aagaagccat ggaagaggag attcgcgagg tgagagacaa ggtttctcat ctttctccag
     1201 aggagcgacc gacggttttt cacctcctct ggcacgatcc tctgtggact gccggcaaag
     1261 atacctttgt taacgagttt atcaccatcg ctggggggca aaatgcattt gctgatttat
     1321 ctggctatgt cattgtggat ttggaagagc ttattcgccg caaccccgac atcatcaccg
     1381 tcgtggaaga tcacggagaa gagggaagcc tctcttacca atttttgctc tccgatagcc
     1441 gccttaaagt agttaacgcc attcaaaatc acaaagtttt tcctgttgat tccgatatag
     1501 tttctcgctc ttctgcccgg gtggtggatg ccgttcgtat atttgcctcc ataattcacc
     1561 cggaaatctt tggagaatat gaataccaga gttaaaaaag ggcttacata tctcggtttt
     1621 ttagctttag tcctggggag cgttttcctg gctcaggggg tgggaggagg gagatatcaa
     1681 ctctctcact ctctctcact cttttttcct tctgaccttt ctccctcgga aaagataata
     1741 ttatttgaga ttcgcctgcc ccgtattgtt ctttcttttc tcgtgggggg aagcctttct
     1801 cttgggggag cagtgttgca ggggattttc cagaatcccc tggtagaccc ctacgtcctg
     1861 ggtatcagct ctggagctgc tctgggagcg gtgattgcca ttttgagcgg cgtgtatctg
     1921 gggtttttca ccgtccctct ttttgctttt tctatggctc tcttcagtgc ttttctggtt
     1981 ttttacctgg gcaaaatagg aaagagattc cctctcgagg ttttgctttt agcaggcata
     2041 ggggtgggat ttttcttctc tgctctggta tctctgggga tgtttttagc cggagaagac
     2101 ctgcaccagc tatttttctg gactatggga gggctgtgga atagcagctg gcaagaggtc
     2161 aaaatgattt tgccttttat cgttgtgggg gctcccattt tgttctcctt tttccgggag
     2221 ctcaatgctt ttctcctggg agaaaaagtg gcagaaagca taggggtaga ggtggaaaaa
     2281 gttaaaaagc aactgctctt tgtggtttcg ctccttgtgg cttccgctgt cgcggtgagt
     2341 ggggttatcg ggtttgtggg gttggtggtg ccacatctgg ttcgccttct gggcgcaaga
     2401 gaccatcgtc atctccttcc tttctccttt cttttaggag ggggagtgct tctgtgggct
     2461 gacactttag ctcgcactat tttatacccg gtagaactac cggtgggaat cataaccagt
     2521 cttctgggtg ctcctttctt tatctatctt ttgcgaaaga ggaagaaaag tctgtgaaag
     2581 aatatttgca ggtggaaaac ctttcttttt cttattcacg gaggaaagtt ttggataaac
     2641 ttaccttcgg cgtgaggagg ggcgagttgg taggggtgat aggcccaaat ggagcaggca
     2701 aaagcactct cctctacctt ttagccggat taattgtccc cgaaagcggg aaaatcgttc
     2761 tgggagaaaa agacaccagg tttctttccc gccgaatgtg gtcacaaaaa gtggctatgg
     2821 tttttcagga aattccccag aacttagagc tcacctctca agaagtaata gccatgggga
     2881 gattccccca catcgggcga tggcagagag aaggagatgt tgatgaggaa aaggtttcct
     2941 gggctatgga gataacctcc actgcagtct tttctgaccg gctgttttcg gagctctcag
     3001 gaggagaaaa acaaagagtg atgattgccc gtgctttagc tcaagaacca gaattgctgc
     3061 tattagacga gcccaccagc cacctggatg ttttacacca gctggaaatt atggacattc
     3121 ttcttgagct cagagcggcg ggggtggcca ttgtttcggt tttccatgat ttgaacctgg
     3181 tttctcagtt ttgtgataag gcgctcctcc tgaaagaagg gagaatgctc tcttttgggg
     3241 gggtggaaga agtgatgaag gcagaggaag tggaaaagtt atttggtgta gagttcctag
     3301 aagccaccca ccctttctct ctccgtccct tttttatgcc tttgcgtaga ataaaaaacg
     3361 aaacgcatcg gggaaaaatc cacctcatct gcggaggggg gagtggctcg cccttgatga
     3421 gagagctttt agaagccggt ttctccctgt ctctgggggt ggttaatcaa ctggattctg
     3481 atgaagagct ggcccgaaag ctgggaattc ctgtggttca agaaaagcct ttctctccct
     3541 tttctccttc tactatggaa gaagcttacc ggttagctca aaaagctgat tttatagtca
     3601 ttgcccccac ttattggggg agaggcaacc tccccaattt agacctggcc gagcgcctcg
     3661 tcttggaagg taaagcagtg ctaattctag aagaggcact gaaccctcgg cttgattata
     3721 ccgaaggagc ggcacaggaa aaattgaaga aaataattga ggagggagga gaggtgataa
     3781 aagacctctc tgcctatttt tcttcctctt cttcttaagc tgccattgca acaatccaat
     3841 aagctggtca ccactcacac cggggcgctt tctcctcctt tgtagtcctc cctctgttat
     3901 tcccgaaatt cagtgaggca tccatctccc tctccccgca agctctctaa accgtcatcg
     3961 cgagcactga gggcttctct tttcgtcatg cctgaaattt agtgaggcat ccaccccctc
     4021 cctccgtcat tcccgaaatc ttttatcggg aatccaaatg cttttaaata cttctggacc
     4081 ccggcttaaa aattgccggg gtgacgaata ag
//
LOCUS       DAOPSS010000137         4060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_17752_length_4060_cov_8.775531, whole genome shotgun sequence.
ACCESSION   DAOPSS010000137 DAOPSS010000000
VERSION     DAOPSS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17752_length_4060_cov_8.775531"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..803
                     /locus_tag="PKN31_07280"
     CDS             33..803
                     /locus_tag="PKN31_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOA99759.1"
                     /translation="MKRNYLFWLGILVFSLAFAGIASASTLDEIKARGKIIIGTDATY
                     PPMEFYDESGEIVGFDIDLGKAIAEELGVEAVFIDTAWDGIFPALDAKKFDIIMSATS
                     ITEERLKSKAMSDPYYITSQAIAVRKDNDSIKGPEDLVGKVIAVQIGTTGDLAVSEIE
                     GVKEIKRFDTTDKAYMEVMNGRADAVVNDLSEVAYRMKFLPDMKIVATFKEGEENYGV
                     TLRKEDTDLLEAINQALKNIKESGKYDQIYQKWFGEIK"
     gene            826..1482
                     /locus_tag="PKN31_07285"
     CDS             826..1482
                     /locus_tag="PKN31_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOA99760.1"
                     /translation="MQFDFLALYEALPALLVGALATLRITALSIAVGLVIGLGAGLAR
                     VWPNSFLRSISSAYIELIRGTPLLVQIFLVYFGLPALGLNLDPFVAGVLAMGINSGAY
                     VGEIVRGGIESIAQGQMEAALSLGMSWWQSMYYVVLPQALFRILPSLGNEFIALLKDS
                     SLVSTIAIAELTRTGQIIITRTFKSFEIWVGVALFYFIMTYAISRAVKYSEKRMRYSE
                     "
     gene            1475..2215
                     /locus_tag="PKN31_07290"
     CDS             1475..2215
                     /locus_tag="PKN31_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOA99761.1"
                     /translation="MSDWVIKVSNLNKYFGSNHVLRDINFQVKFGEVVSIIGASGSGK
                     STLLRCLNGLEPIQSGEIVVDGVKINGEKVDLSRIRQSIGMIFQSFNLFPHMSVLDNV
                     ILAPHRVKKIPRREAEEIALELLGKVGLAEKAHSYPYQLSGGQKQRVAIARALAMNPK
                     VMMFDEPTSALDPETVNDVLDVMKELAKEGMTMIVVTHEMGFAREVSQRVMHIDEGRI
                     LEEGTPEEIFYHPRNPRTRAFLSKVINI"
     gene            2226..3521
                     /locus_tag="PKN31_07295"
     CDS             2226..3521
                     /locus_tag="PKN31_07295"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOA99762.1"
                     /translation="MEDRKVVKIGVIGFGTVGVGTVKILKERKEEIEKELKVAIQIAK
                     IADKDWERERPLTVPPEIRTYDPWEVVKDPEIDIVVEAIGGVSPTFELVSCALEQGKT
                     VITPNKELIAKKGGELLKIAESSNSELFFEGAVGGGIPIIHSLKGQLIGDDIEEVIGI
                     VNGTTNFVLTEMSLHKTSYAEALKEAQNRGYAEPVPVMDVEGFDAAYKIAILASLAFH
                     SKVEVEKVYREGITLLIPEDIEFAKELGFTVKLLAIAKKLNEAMELRVHPVLLPFDHP
                     LSQVYGVENAIYVKSKTRRLTFRGPGAGGEATGSAIVGDIIEAIRNWKWQCRGRVGCT
                     CLREMEVKPITELITQYCVRILALDVPGVLGKIATQFGEAGVSLSAVKQPVSTPGELT
                     NIYFLTHKTKEESLQRSLEHIRKLEVVKEAYAIRVEEGG"
     gene            3537..3851
                     /locus_tag="PKN31_07300"
     CDS             3537..3851
                     /locus_tag="PKN31_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bh protein"
                     /protein_id="HOA99763.1"
                     /translation="MDKEEITTVLRCLSCQRETEHRLIYQGEMVKSIICQECGLEISF
                     DKDRLLALYGEDLVKRVLTKPRRITEEYKEDLVKFFSSIPFRIITKPYRILKEIDSLR
                     DD"
BASE COUNT         1178 a          675 c         1025 g         1182 t
ORIGIN      
        1 ttgtattatt ctcacaaaaa ggaggagctg ggatgaaaag gaattatctt ttttggttgg
       61 ggatattagt attttctctt gcttttgctg gtatagcaag tgcttctact ctggatgaaa
      121 ttaaagctcg gggaaaaatc atcatcggca ccgatgctac ttatccccct atggagtttt
      181 acgatgaaag cggggaaatt gtgggttttg atattgattt aggtaaagcg attgctgagg
      241 aattaggtgt ggaggcagta tttatagaca ctgcctggga tggtattttc cctgctctgg
      301 acgccaaaaa atttgacatt ataatgtccg ctacttctat caccgaagag agacttaaat
      361 ccaaagcaat gagtgaccct tattatatta cctcccaggc tattgcagtg agaaaagaca
      421 atgattctat taaaggtccg gaggatttag taggtaaggt gatagcagtg cagatcggga
      481 ctactggaga tttagcggtg agtgagatag aaggggtaaa ggagataaag agatttgaca
      541 ctactgacaa agcttatatg gaagtgatga atggaagagc tgatgcggtg gttaatgatt
      601 tatccgaagt tgcttaccgg atgaaatttt taccggatat gaaaatcgtg gctactttta
      661 aagaaggaga ggaaaattac ggagttactt tgcgtaaaga ggataccgac cttctggaag
      721 ccatcaatca agctttgaaa aatattaaag agtcgggtaa gtatgatcag atttatcaaa
      781 agtggtttgg ggaaataaag taatttataa gagaggattt aatttttgca gtttgacttt
      841 ttagctctat atgaggcatt accagcactt ttagtggggg cgctggcaac tttacgaatc
      901 accgctcttt ctatagctgt gggtttggta attggtttag gagccggtct tgcgcgagta
      961 tggcccaatt cctttttgcg gagtatttcc agtgcttaca tcgagttaat caggggtact
     1021 ccccttttgg tgcaaatctt tctagtttac tttggactac cggctctggg tttgaattta
     1081 gacccttttg tggccggggt tttagctatg ggaatcaata gtggggccta tgtgggagag
     1141 attgttcgag gaggcataga atctatcgct cagggtcaga tggaagctgc tctttctttg
     1201 gggatgagct ggtggcaatc tatgtattac gttgtattgc ctcaagctct gtttcgcatt
     1261 cttccttcct taggtaatga attcattgct ctccttaaag attcctcttt agtttctact
     1321 attgctattg ctgagctaac tcgcaccggg caaataatca ttacccgtac ctttaagtct
     1381 tttgaaatct gggtgggagt agctctcttt tattttatta tgacctatgc tatttctcga
     1441 gcggtaaaat attcagaaaa aaggatgcgc tacagtgagt gattgggtca taaaagtctc
     1501 taatctcaat aaatactttg gttctaacca tgttttaaga gatatcaatt ttcaggttaa
     1561 gtttggagaa gtggtatcga taatcggtgc ctctggctcg ggtaaaagca ccttgctccg
     1621 ctgtctcaac ggtttagagc ccattcaaag tggagagata gtagtggatg gagtgaagat
     1681 aaacggagag aaagtagatt taagccgaat aaggcaatct atcgggatga tttttcagag
     1741 ttttaatctt tttcctcata tgagtgttct ggataatgtt attcttgctc ctcatagagt
     1801 aaaaaagatt cctcgccggg aagcagagga aatagcttta gagcttttag gtaaagtggg
     1861 tctggcagaa aaggctcatt cctatcctta tcagctttcc gggggacaaa agcaaagagt
     1921 ggctatcgct cgggctttgg caatgaatcc taaagtcatg atgtttgatg aaccgacttc
     1981 ggctctggac ccggaaacag ttaatgacgt tttagacgtg atgaaagagt tagccaaaga
     2041 ggggatgact atgattgtag ttactcatga aatgggtttt gctcgagagg tttcccagcg
     2101 ggtgatgcac atcgatgagg gtagaatatt agaagagggg accccagagg aaatttttta
     2161 tcatccccgt aatcctcgta cccgggcttt tttgagcaaa gtaataaata tttgaaggtg
     2221 ggaaaatgga agatagaaaa gtagtaaaaa tcggcgtcat aggtttcgga acagtggggg
     2281 taggaacggt taaaatatta aaagagagaa aagaagaaat agaaaaagag ctgaaagttg
     2341 ctattcagat agctaaaatt gccgataaag attgggaaag agaaaggccg ctaacagtgc
     2401 ctccagaaat cagaacttat gacccctggg aagtagtaaa agatccggaa attgacatag
     2461 tggtagaggc aataggggga gtgtctccta cttttgagct ggtttcttgc gctcttgagc
     2521 aaggtaaaac agtgattact cctaacaaag aactcatcgc taaaaaagga ggagagttat
     2581 taaaaatagc tgaaagctca aatagtgagc ttttctttga gggagcagtg ggaggaggaa
     2641 tacccatcat tcattctctc aaaggacaac tgataggtga tgatatagaa gaagttatcg
     2701 gaatagtaaa cggcactact aattttgttc tcactgagat gtctctccac aaaacttcct
     2761 atgctgaggc gttaaaagaa gcacagaaca ggggatatgc agaaccagtc ccggttatgg
     2821 atgtagaagg atttgatgct gcctataaaa tagctattct tgcctcttta gcttttcaca
     2881 gcaaggtaga agtggaaaaa gtctatcggg agggaatcac tcttttgatt cctgaggata
     2941 tcgagtttgc caaagaactg ggatttacag taaaacttct ggccatcgct aaaaagttaa
     3001 acgaggcgat ggagctccgg gttcacccgg tgcttttacc cttcgaccat cctctatctc
     3061 aagtatatgg agtagaaaat gccatttatg ttaaatcgaa aacccgccgg ttgacttttc
     3121 gaggtccagg ggcaggagga gaagccaccg gcagcgctat tgtgggagac ataatagaag
     3181 ctatacgtaa ttggaagtgg cagtgccggg gtagagtagg ctgtacttgc ctgcgggaga
     3241 tggaagtaaa gcccatcacg gaattaatta ctcaatattg tgtgcgtatt ttagcattgg
     3301 atgtgccagg agtgctaggt aaaattgcta cccagtttgg agaggcagga gtcagccttt
     3361 cagcggttaa acagccggtt agcaccccgg gtgagcttac caatatttat tttcttactc
     3421 ataaaacaaa ggaagaaagt ttgcaaaggt cgttggagca tattagaaaa ctggaagtag
     3481 taaaggaggc ttatgccatc cgggtggaag aaggaggttg aaaggaggaa aaacttttgg
     3541 acaaggagga gattaccaca gttttgcgct gtttgagttg ccagcgagag actgagcatc
     3601 gcctgattta tcagggagag atggtaaaat ccataatatg ccaggaatgc gggctggaga
     3661 ttagttttga taaagacagg cttttagctc tttatggaga agatttagta aaaagagttc
     3721 ttaccaagcc tcgccggatt actgaagaat ataaggaaga tttggttaaa ttcttcagca
     3781 gcatcccttt tcgtatcatt accaagcctt atcggatatt gaaagaaata gactctctcc
     3841 gggacgactg agaaaataga gctgcagaag cgggtactta tctgttgttt cccttctgtt
     3901 tctcaatgcc ggggtggtgt ttctacggca tagttttttg gtttcaatat tttattttgt
     3961 gccgcaggct gctttgtttt ttaaagggga ttatagggga ataaagtctt ctattccttt
     4021 gagtagttga tctaattcat aactttcctc atctctcact
//
LOCUS       DAOPSS010000138         4005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_18050_length_4005_cov_8.377722, whole genome shotgun sequence.
ACCESSION   DAOPSS010000138 DAOPSS010000000
VERSION     DAOPSS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18050_length_4005_cov_8.377722"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..1306)
                     /gene="tsaD"
                     /locus_tag="PKN31_07305"
     CDS             complement(284..1306)
                     /gene="tsaD"
                     /locus_tag="PKN31_07305"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOA99764.1"
                     /translation="MIHVLGIETSCDDSCVAVINDKYQVLSNVVSSQTEFHRRFGGIV
                     PEVASRKHLEYLPLVLDQALRDAHLDLENIDLVAVTQGPGLLGSLLMGVCLAKTIALF
                     KDKTFLGVNHLEGHLFAIRLEYPEIQPPFLALIVSGGHTEIIAVRDWGKYRHLGSTRD
                     DAAGEAYDKVSKFLNLGYPGGPIIDRLSEGAQGDRFHFSAGLEDDDTLDFSFSGLKTA
                     VVRHYSSLKEEEKKDEKLIRDLVASFQESVARVLVNKTFKALEIYGFSQIVVGGGVAA
                     NRHLRAKMKEEGEKRGVQVFFPSPSFCTDNGAMIAACGLFHWERGRKDSWEMDPDPRL
                     PLTSIE"
     gene            complement(1320..1802)
                     /gene="rimI"
                     /locus_tag="PKN31_07310"
     CDS             complement(1320..1802)
                     /gene="rimI"
                     /locus_tag="PKN31_07310"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOA99765.1"
                     /translation="MAQRSGDREPYLWEEGDFKISWMRPYHLPQVMAIEQSSFPQPWS
                     YSLFLSELSNQAATYFVAFFQGKIIGYLGLWMVWGEAHITTFAIHPAYRGEGFGKKLF
                     GYALNYAKSQGCSEVSLEVRPSNIKAQNLYHSFGFKKIGVRKRYYADGEDAWVMKKVF
                     "
     gene            complement(1780..2448)
                     /gene="tsaB"
                     /locus_tag="PKN31_07315"
     CDS             complement(1780..2448)
                     /gene="tsaB"
                     /locus_tag="PKN31_07315"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOA99766.1"
                     /translation="MILALDSSTSWLSIALGSAEEVFFHLTHYTQKDHSRWLIKHLSL
                     LQKEFNWEKDLEIVLVGLGPGSFTGVKIANIVAKGIAYSLGKPLGGFSTLEVIAHQVP
                     ERIAQDYEILLPVIKHKRKEVFWMELSPQFQECCSFPEIQAGPKEEVATKYEGRNVLV
                     VTPWVDIAEFLESKGFSFFAPRESLPEARGLIELYRRRENEAMVNRENIFTLVPFYGS
                     KVWG"
     gene            complement(2429..2875)
                     /gene="tsaE"
                     /locus_tag="PKN31_07320"
     CDS             complement(2429..2875)
                     /gene="tsaE"
                     /locus_tag="PKN31_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOA99767.1"
                     /translation="MKIIARWQSKSEEETRKWGEKLAKDFLRPGDLVIVAGELGVGKT
                     QLVKGIAKGLGIKEEVKSPSFSLVHEYRKEGFTFYHLDFYRLEKEEEVVDLGWEEYRE
                     DEKGIVVVEWGDKFPFLFSPPFFLVHLSYREEDTRDIEVYYDSCSG"
     gene            complement(2847..3986)
                     /gene="alr"
                     /locus_tag="PKN31_07325"
     CDS             complement(2847..3986)
                     /gene="alr"
                     /locus_tag="PKN31_07325"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOA99768.1"
                     /translation="MENILFPFWMEIDAQNLHNNVEVLKRWSPSSSLMAVVKSEAYGH
                     GWEAVNLLYDYGLRKFGVASVEEAHELRKRGIKGDIYLLGGFFTEEAEMILEDGLTPV
                     VSSPKELQALEELARKADKIIPFHLKVDTGMGRMGFLPQEINGEWWKRWGNNCHLSLR
                     GVMTHFASAESDPAYTHFQFDNFLSFLGRLPENNPSRERHCCNSAAFLAFPEMHLDLS
                     RVGIAFWGVSPWGRKATLPLDLKPVMRIKARVKQVKTLPPGSSVGYGGTFTTSRETKI
                     AIISIGYSQGLFRSLSNRMEVLVRGKRAPSRGNISMDQMTVEVTGIEGVEKGDIATVV
                     GKEGKEEVTIEEIADLAHTVPHEVLCSLGKIKNRMIYENHCSLAK"
BASE COUNT         1200 a         1026 c          703 g         1076 t
ORIGIN      
        1 tcctgagtgg ttctacgagg aatctaaata atgggagggg atgctcctat ggttccaaaa
       61 agaaaaaaag caaaaacgta gattcccgac taaaaatctt gggaatgaca gaagagagta
      121 aatcctctat aacccccaaa aagacaatga atgcaaacaa atgtaaaaaa gagcacaaaa
      181 gacggaaaag taaaagacta aaaattacac tggagaaaaa gattccctct tccttccaga
      241 aagaagagca ggatgagggg tgataatttt cccccttcgg tcttcactct atagaagtca
      301 agggaagtcg agggtcgggg tccatttccc aggaatcttt tctccctcgc tcccagtgga
      361 aaagaccaca ggcggcaatc atagcaccat tatcggtaca aaaggaagga gaagggaaaa
      421 aaacttgcac tcctcttttt tctccctctt ctttcatttt cgctcgcaga tgacgattgg
      481 ctgccactcc tccccccaca actatctgag aaaaaccata aatttccaga gctttaaaag
      541 ttttatttac taaaaccctc gccacacttt cctgaaaaga agctactaaa tctcgaatga
      601 gcttctcatc ttttttttct tcctctttca gagaagagta atggcgcact actgcagttt
      661 ttaatccact gaagctaaaa tccagagtat catcatcctc taaaccagca gaaaagtgga
      721 aacggtcgcc ttgagcacct tcactcaacc tgtctataat aggacccccc ggataaccta
      781 aatttaaaaa cttggatact ttatcataag cttctccagc agcatcgtct cttgtacttc
      841 ctaagtgacg gtactttccc caatcccgga cagcaataat ttcagtgtgc cccccggata
      901 caatcaaggc taaaaaagga ggctggattt ctggatattc gagacgaata gcgaagagat
      961 gcccctccag atggttaacc ccaagaaagg ttttatcttt aaaaagagcg atggttttag
     1021 ctaaacacac tcccatgagc aaagagccca gaagaccagg accctgagtc accgccacca
     1081 gatctatatt ttctaaatcg agatgagcat cccgaagagc ctgatctaaa accagaggaa
     1141 gatactccag atgtttgcga gaggctactt ccgggactat tcctccaaag cgacgatgaa
     1201 attcagtttg actggatact acattggaca aaacttgata tttatcatta atcaccgcca
     1261 cacaactatc atcacaactc gtttctatac ctaaaacgtg aatcaataaa catcctccct
     1321 caaaagacct ttttcattac ccaggcatct tctccatcag catagtagcg cttgcgcact
     1381 cctatctttt taaagccaaa actgtgatac aaattctgtg ctttaatatt ggaagggcgc
     1441 acttccaatg agacctcact acatccttga gatttagcgt aatttagcgc ataaccaaaa
     1501 agctttttac caaacccttc tccccggtag gcaggatgaa tggcaaaagt ggttatatga
     1561 gcctcccccc ataccatcca cagacccaga tagccgatga ttttaccctg gaagaaagct
     1621 acgaaatagg tagctgcctg gttggataat tcactcaaaa ataaagaata agaccagggt
     1681 tgaggaaaag agctctgttc aatggccata acctgaggca ggtgataagg ccgcatccag
     1741 gagattttaa aatctccttc ttcccataaa taaggttctc tatccccaga cctttgagcc
     1801 ataaaagggc accagagtaa aaatattttc tcgatttacc atcgcttcat tctctctccg
     1861 ccgatacaat tctattaatc ctcgtgcttc tggtaaagac tcccggggtg caaaaaaaga
     1921 gaaaccctta ctttccaaaa attccgctat gtccacccag ggagtaacga cgagaacatt
     1981 cctaccttcg tattttgttg ctacctcttc tttaggacct gcttgaattt ctggaaaaga
     2041 gcaacactcc tggaactgag gtgatagttc catccagaaa acctccttcc ttttgtgctt
     2101 tattacaggg agtaaaattt cgtaatcttg agctatacgc tcgggaacct gatgagcgat
     2161 gacctccaga gtagaaaagc cacccagagg ttttcccaga gagtaagcta taccttttgc
     2221 cacaatgttt gcaattttca ctccggtaaa agagcccgga cccaaaccca ccagaactat
     2281 ctccagatct ttttcccagt tgaattcttt ttgtaaaagg ctcagatgct taataagcca
     2341 gcgagaatgg tctttttgag tatagtgggt tagatgaaaa aaaacctctt cagcagaacc
     2401 taaagctata ctgagccagg aagtactgct atccagagca agaatcatag tacacctcga
     2461 tatctcttgt atcttcctct ctataactta agtgaaccag aaaaaaggga ggagaaaaga
     2521 gaaaagggaa cttatccccc cactctacca ccactatccc cttttcatct tcccgatatt
     2581 cttcccatcc taaatctacc acttcttctt ccttttccag acggtaaaaa tctaaatggt
     2641 agaaagtgaa accttctttt ctgtactcat gaactaaaga aaaagaaggg ctcttaacct
     2701 cttcttttat acctaatcct ttagctattc ccttcaccag ttgagtttta cccaccccca
     2761 gctccccagc cactatcacc aggtctccgg ggcgcaaaaa atctttagct aacttctctc
     2821 cccactttcg agtttcttct tcgcttttac tttgccagcg agcaatgatt ttcataaatc
     2881 atccgatttt taattttacc caaactgcac agaacctcgt gaggtacggt atgagctaaa
     2941 tccgctattt cttctatggt tacctcctct tttccttctt ttccaaccac agtagcaatg
     3001 tctccttttt ccactccctc aattcctgtt acctctacag tcatttgatc catagaaata
     3061 tttccccggg atggtgctct tttccccctc actaacacct ccattcggtt agagaggctg
     3121 cgaaacaagc cttgagaata accaatggat attattgcta ttttagtctc ccttgaggta
     3181 gtaaaagttc ccccatagcc cacagaagag ccaggaggaa gagttttaac ctgctttacc
     3241 cgggccttta ttctcatcac tggctttaaa tccagtggca gtgtggcttt tcttccccag
     3301 ggagaaacac cccagaaggc aatccccacc cgggataaat caagatgcat ttccggaaaa
     3361 gcaagaaagg cggcgctatt acaacagtgc ctctcccggg aaggattatt ttcaggaagc
     3421 cgacccaaaa aagaaagaaa gttgtcaaat tgaaagtgag tgtatgccgg gtcagattca
     3481 gcactggcaa aatgagtcat aactcccctc aaggaaagat gacagttatt tccccacctt
     3541 ttccaccatt ccccatttat ctcttgaggt aaaaatccca ttcgccccat cccggtatct
     3601 actttcaggt gaaaaggtat gattttatct gcttttctgg ccaactcttc cagagcctgc
     3661 agttccttcg gggaggaaac caccggagtc aacccatctt ccagaatcat ttctgcttcc
     3721 tcagtgaaaa atcctcccag aagatatata tcccctttta tacctctttt cctcaactca
     3781 tgcgcttctt ctaccgaagc cactccaaat tttctcaacc cgtaatcata aagaagattt
     3841 actgcttccc atccatgacc ataagcctcc gactttacta ctgccataag ggaagaagag
     3901 ggagaccacc tcttcaatac ctctacattg ttatgcaaat tctgagcgtc aatctccatc
     3961 cagaaaggaa agagtatatt ctccattttc taccatcctt cgagc
//
LOCUS       DAOPSS010000139         3928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_18443_length_3928_cov_6.267235, whole genome shotgun sequence.
ACCESSION   DAOPSS010000139 DAOPSS010000000
VERSION     DAOPSS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18443_length_3928_cov_6.267235"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(680..1537)
                     /locus_tag="PKN31_07330"
     CDS             complement(680..1537)
                     /locus_tag="PKN31_07330"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOA99769.1"
                     /translation="MARSLMVQGTGSGVGKTVIVAGLVRVFARRGIKVAPFKAQNMSC
                     SSGITEKGEEMSIAQILQARAAFQKPDSRMNPVLLKPQGERKSEIIVRGKAWSIKEAS
                     AYYRDNEFLWEVVRESLDSLMQEYDLLILEGAGSPAEINLRGKDITNMRVALYLQSPV
                     IVVGDIEKGGVFASLYGTWALASEEEKKLMRAFIINKFRGDEEILKPGLQEMEELTGV
                     PVVGVLPYVSLSLVEEDSLSGESSSKDCFSCLRGDFSWEEIDQELDWWSKTLEEYLDI
                     KKIEEIIGL"
     gene            complement(1538..2806)
                     /locus_tag="PKN31_07335"
     CDS             complement(1538..2806)
                     /locus_tag="PKN31_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA99770.1"
                     /translation="MARRKKGIVLSRRDYLFNFVVDTLDYSFFSLGMSFGSTVTLLPL
                     LAQRLGASNLEIGLIPAIAYLGWSFPAIWGARVSGKLEKKLPFILRFTLFERLPFLGM
                     ALLAFYLVPHNPSLSLYILFILLGINSFAMGFLGPIWTEMIGKVIHHSRWGLYFACGN
                     GLGALMGMWGSTLAERFISTYPFPRNFGHCFLLASVSMVVSYFFLALTREEKEEVTSS
                     SGEKFLSSFFQVIREDANFLSFLLARIFLALGVVGGAFYTVYALQKFIIPDALVARYN
                     AVLLVSQALSNFIWGPLGDKKGHKLVVLLGATSIVLSNLLALFSPDFRWFFLAFTLLG
                     FNYSAISVGGTAIVLDFAPREKRGLYVGWSSFLSNFPAFFSPILGGKIADRFGYHWVF
                     WLALSLNLVGLLWILWGVREPRAFTNFEEG"
     gene            complement(2782..>3928)
                     /locus_tag="PKN31_07340"
     CDS             complement(2782..>3928)
                     /locus_tag="PKN31_07340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOA99771.1"
                     /translation="FVNIMRFPERYPDCQIFKLETNYRSRAPILELANQIISFNRRQF
                     PKVLHPMKKGGEKPVVVPLRDVMEQAEFVASQILELHDEGHDLQEIAVLYRAHYHSME
                     IQMELTRRGIPFEIRSGLRFFEQAHIKDVVAYLKVVANPRDEMAWKRVLKLYPGIGRA
                     LAEKVWGALSSSPDPLTTLEREEVFSLLPVNARASLASLRDLLFHLEDLDSSPSTLIQ
                     EVLAGGYRDYLVAHYPNYEEREEDLEELANFALRYKSLETFLSELALLGEIEAENVVR
                     GGIEDEKIVLSTIHQAKGLEWDCVFVVWLCEGGFPSPRSLGREENIEEERRLFYVACT
                     RAREHLFLCYPILGERGRSRSMVRKPSRFLQELDPRSYTKWRGGRKG"
BASE COUNT         1295 a          982 c          738 g          913 t
ORIGIN      
        1 gtgtaaaaca agttaccaga taaactatgc aaggataacc cgctaaacta tcacctgaat
       61 ggctctatcg actatgaggg gacgacgccc ctttgtagcc cccctgaaaa agcaaaaaca
      121 caaagacaaa aacaaaaaga aaaggcaaaa acgtggattc ccgataaaag atttcgggaa
      181 tgacggcggg ggaggtcgtc acccctgaat gcccctatca ggggtccaca gtatttgaaa
      241 gcatttggat tcccgattaa agatttcggg aatgacaaaa aaagaggcgt cattcctgaa
      301 tggttctacg aggggtctaa ataatgggag ggaacacttt tatggttcca aaaagaagaa
      361 gcaaaaacgt ggattcctcg ctaaatttca ggcatgacag aaagggaaac ccttccagag
      421 agcggtgaaa ccaaactatg aggaggcaaa acactcctca taacccccag gagactaaaa
      481 cagagcagcc tacggcgtaa gaccaagaaa acaaaaacct aaaaagcaaa gactactggt
      541 gggaaacact ccaccagtat aagggggaaa gaattccccc ttatgaagcc gacaataggg
      601 ggtaaacccc ttataacccc aaaaaaaaac aaaaagcaaa aactgctgag ggagggaaac
      661 tctccagcat gcctcatcgt cataaaccaa ttatttcctc tattttttta atgtctaaat
      721 attcctctaa ggttttactc caccaatcca gttcctggtc tatttcttcc caggaaaaat
      781 ctcccctcag gcaggaaaag cagtctttac tactcgattc ccccgagagg ctatcttctt
      841 ccactaaaga tagagaaaca tagggtaaaa cccccaccac cggaaccccg gtgagctctt
      901 ccatttcctg taaaccgggc tttaaaattt cttcatctcc ccgaaattta ttgataataa
      961 aagcccgcat cagctttttt tcctcttcac tcgccagagc ccaggttccg tataaagagg
     1021 caaaaactcc tcctttttct atgtccccca ccacaataac tggagattga agataaagag
     1081 ccactctcat gttagttatg tcttttcccc gcagatttat ctctgctggg cttcctgctc
     1141 cctctaaaat aaggaggtca tattcttgca tcagagaatc aaggctttcc ctgaccactt
     1201 cccacaaaaa ttcattgtcc ctataataag cagaagcctc ctttatgctc caggcttttc
     1261 ctcggactat tatctcgctt tttctctccc cttgaggttt caaaagaaca ggattcatcc
     1321 gactatcagg tttttgaaaa gcagctcgag cttgaagaat ttgagctatg gacatttctt
     1381 ctcctttttc agtaattcca gaactacaag acatgttctg agctttaaaa ggagctacct
     1441 ttattccccg ccgggcaaaa actcgtacca accctgccac tatcacagtt ttacccactc
     1501 ctgatcctgt tccctgcacc attaaagacc tggccattta tccttcctca aaattagtaa
     1561 aagctcgagg ctccctcaca ccccataaaa tccacagcaa ccccaccaaa ttcagactca
     1621 gcgctaacca gaaaacccag tggtaaccaa atcggtcagc aatttttccc cctaaaatgg
     1681 gagagaaaaa agccgggaaa ttacttaaaa agctactcca ccccacatac aaccctcgct
     1741 tttctcgggg agcaaagtca agaacaatgg ctgttcctcc caccgatata gcactataat
     1801 taaatcccaa aagggtaaaa gctaagaaaa accatcgaaa atcaggagaa aaaagagcca
     1861 gcaaattgct caaaactata gatgtagctc ctaaaagcac cactaattta tgcccttttt
     1921 tgtctcctaa gggaccccag ataaagttac tcaaagcctg agagacaagc aaaacagcat
     1981 tatagcgagc cactaaagca tcagggatta tgaatttttg caaagcataa acggtataaa
     2041 aagcccctcc tactacccct aaagctaaaa atatacgagc cagcaaaaaa ctcaaaaagt
     2101 tggcatcttc tcttattacc tggaaaaaag aagagagaaa tttttctcca gaggaagaag
     2161 ttacctcctc tttctcttcc cgggtaagag ctagaaaaaa ataagaaacc accatcgata
     2221 cagaagctaa caggaaacaa tgaccaaagt tgcgaggaaa aggataagtg ctgataaatc
     2281 gttctgccag agtagaaccc cacattccca tgagagcccc caggccgtta ccacaggcaa
     2341 aatagagtcc ccagcgactg tgatggatga cttttccaat catctccgtc cagataggac
     2401 cgagaaaccc catagcaaaa gaattaattc ctaacaaaat gaacaagata taaagcgaaa
     2461 gagaaggatt atgaggaacg agataaaaag ccagaagcgc catccccagg aaaggaaggc
     2521 gctcaaaaag agtgaatcga aggataaaag ggagtttttt ctctaattta ccagaaaccc
     2581 ttgcccccca gatagcagga aatgaccatc ccagataagc aatagcagga atgagcccga
     2641 tttccaaatt gctggcccct aagcgttgag ccagaagggg aagcagggta actgtagagc
     2701 caaaagacat ccccaggcta aaaaaagagt aatccagagt gtctactaca aagttaaaaa
     2761 gataatctcg ccgggagagc actatccctt tcttcctcct cgccatttag tgtaggaacg
     2821 ggggtctaac tcctgcagga atcgagaagg tttcctgacc atagagcgag aacgccctct
     2881 ttctcctaaa ataggataac acaaaaataa atgttctcga gctctggtac aggccacata
     2941 gaaaagtctt ctttcttctt cgatattctc ctctcttccc agactacgag gagaaggaaa
     3001 acccccctca cataaccaga ctacaaacac acaatcccac tccagacctt tagcctgatg
     3061 aatggtggaa agaactattt tttcatcctc tattcctccc cttaccacgt tctctgcttc
     3121 tatttctcct aaaagagcta actcacttaa aaaagtttct aaagatttat accgcaaagc
     3181 aaagttagcc agctcttcca ggtcttcctc tctctcctcg taattaggat aatgagcaac
     3241 taaatagtcc cggtatcccc ctgcgagcac ctcctgaatt agagtagaag gagaggaatc
     3301 gaggtcctcc agatgaaata gtaaatctct aagagaagcg aggctagccc gggcgtttac
     3361 cggaagaaga gaaaaaactt cctctctctc cagagtagta agaggatcgg gagaggaaga
     3421 aagagctccc catacttttt ctgctaatgc tcgaccaatc ccgggataaa gcttgagcac
     3481 tctcttccag gccatctcat cccggggatt agccactacc ttaagataag ccaccacatc
     3541 ttttatatga gcttgttcaa aaaaacgcag tccggagcga atttcaaaag ggatgccccg
     3601 gcgagtaagc tccatttgaa tttccatgct gtgataatga gcccggtata aaacggcaat
     3661 ctcctggagg tcatgaccct catcatgtaa ttctaaaatc tgggaagcta caaattcggc
     3721 ctgctccata acatcacgca agggcaccac tacaggcttt tctcctcctt ttttcatcgg
     3781 atgtaaaact ttaggaaatt gacgacgatt aaaagaaatt atctgattgg ctagctccag
     3841 aataggagct ctacttcgat agttggtctc cagtttaaat atctgacaat ccgggtatct
     3901 ctcagggaaa cgcatgatat tgacaaaa
//
LOCUS       DAOPSS010000140         3920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_18495_length_3920_cov_9.097801, whole genome shotgun sequence.
ACCESSION   DAOPSS010000140 DAOPSS010000000
VERSION     DAOPSS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18495_length_3920_cov_9.097801"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..696
                     /locus_tag="PKN31_07345"
     CDS             52..696
                     /locus_tag="PKN31_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOA99772.1"
                     /translation="MQLKSRQEVNQFLEKSKIIAIIRAQEAQSLTEAVTALREGGINC
                     IEVTMTTPGALNILEEVSNKVEGVLLGAGTVLDSETARLCILSGARFLVTPSLNFSVI
                     EMAHRYDVPIIPGALTPTEILGAWEKGADVVKVFPAGSLGPGYIKDLKGPFPQIKLCP
                     TGGVNLDNLSSFLKAGASCVGVGGALVKNEFLREKKWGELKELARKFQEAAQNS"
     gene            complement(693..1178)
                     /locus_tag="PKN31_07350"
     CDS             complement(693..1178)
                     /locus_tag="PKN31_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOA99773.1"
                     /translation="MRRNRRNSISSKNRWLYYLIIILFLFFYFQSEEKGAYLVARIID
                     GDTIELSNGEKVRYIGIDTPELHHPQKEVEYYAREAYEANRRLVEGKRVRLELDVEER
                     DRYGRILAYVYVDDLMVNEWLVANGYARVATFPPNVKYAERFLQLEREARQAKIGLWA
                     D"
     gene            complement(1175..2113)
                     /locus_tag="PKN31_07355"
     CDS             complement(1175..2113)
                     /locus_tag="PKN31_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOA99774.1"
                     /translation="MSQVVVLARSFSRGSNEPRELLSQAGLEVVFKKNPEPEKEEKVA
                     ELIGDAEAVIVGVDRIGEVVFSRCSNLRVVSKHGVGVDNIDLEAAKRHGVVVANAPGT
                     NSQSVADMAFLLILACARGLPILLEQVKNKVWSSPFLGVELEDKVLGIVGLGRIGKEV
                     GKRGQGFGMKVIYYDPVVEDKEFTSVSLEDLFKSADFVSLHAPLTKDTEKMVGEKLLS
                     LMKEEAFLINTARGELVDEEALYRFLQEKRIKGAALDVLTFEPPFESPLLSLPNVIVT
                     PHVSAHTKEANIKMGRIAALNVIRVLQGEEPLYRVV"
     gene            complement(2110..2667)
                     /locus_tag="PKN31_07360"
     CDS             complement(2110..2667)
                     /locus_tag="PKN31_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOA99775.1"
                     /translation="MVWIILLVVAILVIYLIVLYNRLVVYKNRVDNSWSQVEVQLRRR
                     YDLIPNLVETVKGYASHEKEVLENVTRARQMAMGAQTPQERGEAEEGITRALRQLFAV
                     VEAYPALRANENFQSLQKELSETEDKIAYARQFYNDTVYKYNTMIEKFPANIFARSLG
                     FSRREYFEAGEGRGPVQVNFREEGK"
     gene            complement(2682..>3920)
                     /locus_tag="PKN31_07365"
     CDS             complement(2682..>3920)
                     /locus_tag="PKN31_07365"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOA99776.1"
                     /translation="LEPHIFPLLPLLAENRYEKILQEEEKLISQGPQVSGLTVFLSFL
                     LVLGGIFYFLLFFYYYFRYGKEPQVDYEREYEQEPPRDISPSLLGSILNQQGASPSIM
                     SRAFLATILDLARRGYLEVEEKEEKKLIGKKDYLNFTFTGKGASKEKDDALLPLEQEV
                     LDLLHRISKDGKNVSTLDIEEWGKEFRGDKSNFLYFIEKWGEEARSFWEEHFFPLLDP
                     ISEVKKTQFFVLGMIYSGIAIFYVFFALTSGNSFWGTFLVIPFIPLTLFLAFLSQKSL
                     SRWSREGALEFKRWQAFRRFISDFSLMREAPSILLHIWDRYLVYAVVLGVAEELLKNL
                     KLYAQERGETLVVPAWYHSGDAAFTLASLDGLARLNNFSNRMENLVHLRQAVSTSTSM
                     GGGFSHGGGGGGGGGSSKAG"
BASE COUNT         1185 a          943 c          707 g         1085 t
ORIGIN      
        1 agattttact ggtaaaatat tgacgaaagc caatttagga ggtagatgag gatgcaactt
       61 aaaagccgtc aggaagttaa tcaatttctg gaaaaaagta aaatcattgc catcatcaga
      121 gctcaggaag cacaatcact tactgaagcc gtaacagcgc tccgagaggg aggaataaac
      181 tgcattgagg ttacgatgac cacccccgga gcactaaata tcttagaaga agtaagtaat
      241 aaagtggaag gagtgctcct tggcgccgga acagttctcg actcagaaac ggcgcgattg
      301 tgcattttaa gtggagctcg atttttagtt actccttctc tcaacttttc cgtgattgaa
      361 atggctcatc gctacgatgt tcccataatt ccaggagcct taactcccac tgaaattctt
      421 ggagcctggg aaaaaggtgc agatgtggtt aaagtatttc ctgctggaag cttaggccca
      481 ggctatatta aagatttaaa agggcccttc ccccagatca agctttgtcc caccggagga
      541 gtcaatctgg ataatctctc ttctttctta aaagcaggag ccagttgtgt gggagtggga
      601 ggagcgttgg ttaagaacga gtttctccgg gagaaaaaat ggggagagct aaaagaactg
      661 gccagaaagt tccaggaagc agcccagaat agctaatcgg cccacaaacc aattttcgcc
      721 tggcgcgctt ctctttccag ttgtaaaaag cgctcagcat actttacatt aggaggaaag
      781 gtggccacac gggcataacc gttggccacc agccattcat taaccattaa atcatccaca
      841 taaacatagg ctaaaattct cccataacga tctctctctt ccacatccaa ttccagccgt
      901 actctttttc cttctaccag ccgacgatta gcttcatagg cttcccgagc atagtattct
      961 acctcttttt gaggatgatg aagctcgggg gtgtctattc caatatagcg cactttttct
     1021 ccattagata gctctatagt atctccatct ataattcttg ctacaagata cgcacctttt
     1081 tcttcacttt gaaaatagaa aaagagaaaa aggataatta ttaaataata tagccaccta
     1141 tttttggaag agatagaatt gcgccgattt ctcctcatac cactcgatat agaggctctt
     1201 ctccctgtaa aacgcggatt acatttaaag ccgcaattct tcccattttt atattggctt
     1261 ctttagtgtg agcagaaaca tgaggagtaa caataacatt aggaagagat agaagaggac
     1321 tctcaaaagg aggctcaaaa gtcaaaacat ccaaagctgc acctttgatt cttttctctt
     1381 gcaaaaatcg gtaaagtgct tcctcatcca ctaattctcc tcgagcagta tttataagaa
     1441 aagcttcttc tttcatcaag gatagcagct tctctcctac cattttttct gtatccttcg
     1501 ttaagggagc gtgaagactg acaaaatctg cgcttttaaa taaatcttcg agagaaaccg
     1561 aagtaaattc tttatcttcc accaccgggt cataataaat caccttcatt ccaaacccct
     1621 gacctctttt tcctacttct ttgccaatac gtcccaaacc tactatgcct aaaactttat
     1681 cctctaactc cactcccaga aaaggggaac tccagacttt attttttacc tgttctaaaa
     1741 gaataggaag ccctctggca caagccagga ttaaaagaaa agccatatct gccactgatt
     1801 gggagttagt gccgggagca ttagccacca ctaccccgtg tcttttggct gcttctaaat
     1861 cgatattgtc cacccccaca ccgtgcttag aaaccacccg taggttggaa cagcgggaaa
     1921 aaactacctc tcctatccgg tctaccccta caatcactgc ctctgcatct ccgataagtt
     1981 ctgctacttt ttcctctttt tctggttcag ggtttttctt aaatactacc tctaaaccag
     2041 cttgactcaa tagttctcga ggttcatttg aaccccgaga aaaagaacgg gccagtacta
     2101 ctacttgact cattttcctt cctccctgaa gttaacctgc actggacctc tgccctctcc
     2161 cgcttcaaaa tattctcggc gggaaaatcc caaacttcga gcaaagatat tagcagggaa
     2221 tttttctatc atagtattat acttataaac cgtatcgtta tagaattggc gagcataagc
     2281 aattttatct tcggtctcag aaagttcttt ctgcaggctc tgaaaattct cattggctcg
     2341 taaagcagga taggcttcta ccacagcaaa caattgcctt aaagctcgag ttataccttc
     2401 ctccgcttct cctcgctcct gtggagtctg agctcccata gccatctgtc gagctcgagt
     2461 tacattttct aacacttctt tctcatgaga agcataacct ttaactgttt ctaccaggtt
     2521 agggataagg tcatagcgac ggcgtaactg tacctctacc tgagaccagc tgttatccac
     2581 ccggtttttg taaactacca aacgattata aagaacaata agataaatta ccagaatcgc
     2641 cactaccagt aaaataatcc agaccatcta tattcctcct ttcaacctgc tttggaactt
     2701 cctcctcccc ctccaccacc tccaccatga gaaaaccctc ctcccatgga agtagaagta
     2761 gaaaccgcct ggcgtaagtg aactaaattt tccatccggt tagaaaaatt atttaatcgg
     2821 gctaacccat cgagagaagc taaagtaaaa gccgcatctc ctgaatgata ccaggcggga
     2881 accactaaag tctctcctct ttcttgcgcg taaagcttta gatttttcaa aagttcctct
     2941 gccactccca gtactacggc ataaacgagg taccggtccc agatatgaag taaaatagaa
     3001 ggagcttccc tcatcagaga aaaatcagaa ataaacctcc ggaaagcctg ccagcgctta
     3061 aattccagtg ctccttctct actccaccgg ctaagagatt tttgagataa aaaagctaag
     3121 aataaagtca gaggaataaa aggtataacc aaaaaagttc cccaaaagct atttcccgag
     3181 gttaaggcaa aaaagacata aaatatcgct atcccgctat aaatcatccc cagaacaaaa
     3241 aactgagtct tcttaacctc tgagattggg tctaaaagag ggaaaaagtg ttcctcccaa
     3301 aagcttcttg cttcttctcc ccatttttct ataaaatata aaaagttact tttatccccc
     3361 ctgaattcct taccccactc ttcaatatcc aaagtagaaa cattcttccc gtctttagaa
     3421 atacggtgca gaaggtctaa aacttcttgc tctaaaggta acagagcatc atccttctct
     3481 ttagatgctc ctttcccggt aaaagtaaaa ttcaaataat cttttttgcc tataagcttc
     3541 ttttcctctt tttcttctac ttccagatat ccccgacgag ccaggtctaa aatggtagct
     3601 aaaaaagctc tactcattat cgagggagaa gctccctgct gatttaatat gcttccgaga
     3661 agagaaggag aaatatcccg gggaggctcc tgttcatatt ctctttcata gtccacttgg
     3721 ggttctttac cgtagcgaaa atagtaataa aagaaaagaa ggaaataaaa aatacctccc
     3781 aaaactaaaa gaaaagagag aaacactgtc aatccggaaa cctgcggtcc ctggctgatg
     3841 agtttctctt cttcttgaag aattttttca tatctatttt cagccagaag aggaagaaga
     3901 ggaaaaatgt gcggttcaag
//
LOCUS       DAOPSS010000141         3847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_18977_length_3847_cov_8.121308, whole genome shotgun sequence.
ACCESSION   DAOPSS010000141 DAOPSS010000000
VERSION     DAOPSS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18977_length_3847_cov_8.121308"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1747)
                     /locus_tag="PKN31_07370"
     CDS             complement(8..1747)
                     /locus_tag="PKN31_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA99777.1"
                     /translation="MKKQSSAFSRIWGYLRPYKVYVIGAFIMALLGAGFNLVVPWLVK
                     GAIDDALIGRDNRLLLFIALGAVILFFFKGLASYLQNLWVSFSGFRATTRLRSELYQH
                     LYFLPASFFRDNPAGEVVSRLTNDVAILQNLFSNTFVNLFMDFFIFSGSIILLFLIHW
                     KLALFSVAVLPLVGFSVEYLGKKVKGVSHLLQKRVASLASLVERAMAGMKIFQSYMTG
                     NYEIEKFEAENEASFDLAMEQVRAKALLTPLVELVSACGVTAVLLYGGREVIQGYLTT
                     GELIAFLGYLLTASTPLSRFSGSFQVLQQSLASAERLFDFLDLPLWGKEMEGQKEITS
                     LQEGIKFKNVSFSYQEEKVLENFNLEIKKGEKVGIVGRSGAGKSTLINLLLRFYDPDE
                     GNIEIDSVDIRELTFSSLRSLMGVVLQDTLILGGTIRENILYGNLDATSEEVRLASVR
                     AHAHEFIVHLEKGYDTEVGEGGARFSGGQKQRIAIARALLKDPPILIFDEATSNLDPE
                     SEKCILDTIANIEEDKIVIVVAHSLSMVKDLDRIIVIEDGRVRGDGTHEELLQSNLYY
                     RRLFGVETFDKAL"
     gene            complement(2204..3304)
                     /gene="lpxB"
                     /locus_tag="PKN31_07375"
     CDS             complement(2204..3304)
                     /gene="lpxB"
                     /locus_tag="PKN31_07375"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOA99778.1"
                     /translation="MGKVFVSIGDVSGDYYGGEIIYLLRQKSEETEFLALGGEKSEKA
                     GARLLYPTASISTVGFWEAVFTFREWEKVWRLSQEAIEREHPDVVLLIDNPGFNLRLA
                     SFCHKKKIPVIYFVPPQVWAWGRKRASRLSQCADWILTIFPWEVDYFQGGRAQVEWVG
                     HPATHLIPQAQFSRLEGDKKRIILLPGSRKKEIINYLTVIKKFIPLLLREREAEFTMI
                     AASQEIQKLLEKEMVDFPVTIKERKELLSVLPGSSLAISSSGTITLEVALAGVPQIIV
                     YRVAFLTFLLARLLMEKTFIGLPNIVMEEEISPELVGRDFSSSRLLREVEKILSDSRS
                     GEKALEFSLKIREKLNRGNPFEEVAQVVSEYL"
     gene            complement(3305..>3847)
                     /locus_tag="PKN31_07380"
     CDS             complement(3305..>3847)
                     /locus_tag="PKN31_07380"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HOA99779.1"
                     /translation="EENVLHEELERLFFSIPKWQDQALLQAVLTKFQEKVELISPLFF
                     LKEYLTPPGKIAGRDPSPEEWKDISYGVRIARFLADEEIGQTVVVKRGTVLALEGAEG
                     TDETIKRGVSLGKEVVVVKMARSQQSFLIDIPAIGEKTVQCLKNGGIIALEAGKTFLL
                     DRENIEEEARNLGVGIVGIN"
BASE COUNT         1204 a          917 c          646 g         1080 t
ORIGIN      
        1 cggaaaatca gagagcttta tcaaaagtct ctacaccaaa aagacgtcga taataaagat
       61 tagattgtaa aagctcttcg tgtgttccgt cacctctaac tcttccatct tcaataacaa
      121 taatccggtc taaatctttt accatactca aggaatgagc cactactatt actatcttat
      181 cttcctcaat gttggcaatg gtatctaaaa tacatttctc cgattcagga tccagattag
      241 aggtagcctc atcaaaaatt aaaataggag gatcttttag caaagctcga gcaatggcaa
      301 ttctctgttt ttgtcctccg gagaagcgag ctcctccttc tcccacttca gtgtcataac
      361 ctttttctag atggacaata aattcatgag cgtgagctct aacagaagca aggcgaacct
      421 cttctgaggt ggcatctaag tttccatata ggatgttttc tcttatcgtt ccccctaaaa
      481 tcagagtgtc ctgtaatact acccccatca aacttctcaa cgaggaaaaa gtcaattctc
      541 gaatatcaac actatcaatt tctatgttcc cctcatccgg gtcataaaaa cggagaagta
      601 agttaattaa cgtactcttt cccgctccgc ttcgtcccac tatccccact ttttctcctt
      661 tttttatttc tagattaaaa ttttccaata ctttttcttc ctggtaagaa aaagaaacat
      721 tcttgaattt tattccttct tgcaaagaag ttatttcttt ttgtccttcc atctctttac
      781 cccacaaagg aagatctaga aaatcaaaga gtcgttcagc agaagccaaa ctctgctgaa
      841 gaacctggaa gctgccggaa aaacgtgaga gaggagtgga agcagtaagt aaatatccca
      901 gaaaggctat taactcaccg gtagtaaggt aaccttgaat aacctctctt ccaccataca
      961 ataaaacagc ggtaacacca caagcagaga ccaattctac cagaggggta agtaatgcct
     1021 tagctcgaac ttgctccatg gccagatcaa agctggcctc attttctgct tcgaattttt
     1081 cgatttcata attgccagtc atataagatt gaaaaatctt cattcccgcc attgctctct
     1141 ctactagaga agccaatgat gccactctct tttgcaagag atgagaaact cccttaactt
     1201 ttttccccag gtactctacg ctaaaaccca ccagaggtaa aaccgctact gaaaacagag
     1261 ccagcttcca gtggataaga aacaacaaaa ttatagagcc agaaaaaata aaaaagtcca
     1321 taaacaggtt aacaaaagta ttagaaaaaa gattctgtaa aattgccaca tcattggtga
     1381 ggcgagacac tacctctcct gcaggattat cgcggaaaaa agaagcagga agaaaataaa
     1441 gatgctgata caattcagaa cggagacgag ttgtggcccg aaatccagaa aaagaaaccc
     1501 ataagttctg aagataagaa gccaatccct taaagaaaaa aagaattact gcccccagag
     1561 caataaaaag aagaagccgg ttgtctcttc ctattagagc atcatcaatg gcacctttaa
     1621 ccagccaggg aacaaccaaa ttaaatcctg ctcccaagag agccatgata aaggcaccta
     1681 tcacatagac tttataagga cgaagatacc cccagatacg gctaaaagca ctactttgct
     1741 ttttcattag tccactctct gagcatttcc gccaggcgtg gagatacacc gcgtagagag
     1801 cgctccttgg gaaagtaaat tcctggttcc ttattcaaca ctccttaaat tacctccagt
     1861 gctttcgata caaatagatt tcccttaatt cctaaaagtt catagaaaga gaaatccgct
     1921 tccacccctc agctaatttc ttccttccac tattttaccc taaaaaggga ttttttccta
     1981 atcttcggaa aagaagaatc tgtgtacaaa tctacctttc gcaatcattt aaaataaccg
     2041 ccatttatca cttcttcctc tcaaccggta ctaatacggg aaaggagaga aataaaccca
     2101 tcctcgagga ggaaaaaagc tgatgcaaaa gatcatgtaa aagtgagtac aatcaataat
     2161 atacttttcc tggctattca cagttacggg caataaagaa tggtcagaga tattctgaca
     2221 ccacctgagc tacttcttca aaaggatttc ctcgattcaa tttttccctg atttttagag
     2281 aaaattcgag agccttttcc cccgaccttg aatcacttaa tattttttct acctccctga
     2341 gaaggcgaga agaagaaaaa tctctgccaa ctaattcagg agatatttct tcttccatca
     2401 caatgtttgg caaacctata aaggtttttt ccatcaacag tcgagctaaa agaaaagtta
     2461 agaaagccac ccggtaaact atgatttgag gaactcctgc cagagccact tctagagtta
     2521 tagttccaga agaggatata gctaaagaac tacctggcag gacggaaagt aactcttttc
     2581 tctcttttat agtcactgga aaatcaacca tttccttctc taaaagcttt tgtatttctt
     2641 gtgaggcagc aatcatggta aattctgctt ctctctctct caagagaagt ggaataaatt
     2701 ttttaatcac tgtcaaataa ttaataattt cttttttccg gctaccaggt aaaaggatta
     2761 tcctcttttt atccccttcc aaacgggaaa attgggcttg aggaataaga tgggtggcag
     2821 gatgccctac ccattctacc tgagctctac ctccctggaa gtaatccacc tcccatggga
     2881 aaatagttaa aatccaatct gcacattgag aaagacgtga agctcttttc ctcccccacg
     2941 cccacacctg aggaggaaca aaataaatca caggaatttt tttcttgtga caaaaagaag
     3001 ctaatcggag gttaaaacca ggattatcaa ttaataatac tacgtcagga tgctctctct
     3061 ctattgcttc ttgagataag cgccacactt tttcccattc ccggaaagta aataccgctt
     3121 cccaaaaacc cacggtagag atagaggccg tagggtaaag aagtcgggca ccggcttttt
     3181 cgcttttttc tcctcccaga gctaaaaatt ctgtttcttc gcttttttgc cgaagaagat
     3241 aaattatttc tcccccgtag taatcgccag aaacatcacc aatagaaaca aaaaccttgc
     3301 ccattcaatt aatccctact attcccactc ccaaatttct tgcttcttct tctatgtttt
     3361 cccggtccag aaggaaagtc tttcccgcct cgagagcaat gattcctccg tttttaagac
     3421 actggacggt cttctctcct atcgctggaa tatcgatcag aaaactttgc tgactacggg
     3481 ccattttaac taccactact tctttgccca gggaaactcc ccgcttaatg gtttcatctg
     3541 ttccttctgc tccctccaga gctagtaccg tcccgcgttt cactaccacg gtttgtccaa
     3601 tttcttcatc agccaaaaaa cgggcaattc gaactccata ggaaatatct ttccactcct
     3661 ctggcgacgg atctctccct gctattttcc ccgggggggt gaggtactct ttcaagaaaa
     3721 agagaggaga aatcaactca actttttctt gaaattttgt aagcacagct tgtaaaagag
     3781 cctgatcctg ccatttagga atagaaaaaa atagcctttc caattcttcg tgaagcacat
     3841 tttcttc
//
LOCUS       DAOPSS010000142         3806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_19263_length_3806_cov_8.185017, whole genome shotgun sequence.
ACCESSION   DAOPSS010000142 DAOPSS010000000
VERSION     DAOPSS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19263_length_3806_cov_8.185017"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      104..244
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            327..896
                     /locus_tag="PKN31_07385"
     CDS             327..896
                     /locus_tag="PKN31_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOA99780.1"
                     /translation="MEQKKLVYGGALAGLSLVVSLLIHFPLIPQAPFLLYDPGDIPIL
                     VAGFKFGPGLGLLLTAVVAVLFALITGQGGPWGVLMHFLATGSYVAVAGFIYQRRRTQ
                     NGAIISLIVAPLFMTVIMAGANLLVTPLYMGVGREVVKGMLLPAIIPFNLLKGVINGA
                     ITLLVYKRISNFIEEPLYKRGKATTRVRS"
     gene            complement(855..1373)
                     /locus_tag="PKN31_07390"
     CDS             complement(855..1373)
                     /locus_tag="PKN31_07390"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOA99781.1"
                     /translation="MRTWLGFSLVSWQEDRLRAFILPQKSKEKALSIFQKKAGFSFCL
                     RPQEKSPFPYLEEAIQNYFEGYPVSFSCPFYLDDLTPFTREVLKATALIPYGKTLTYG
                     QLAEKIGNPRASRAVGRALGQNPLPLLIPCHRVTARYNWGGYSTYGLRFKILLLSIES
                     RPDPGSSFTPFI"
     misc_binding    1469..1684
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1732..2745
                     /locus_tag="PKN31_07395"
     CDS             1732..2745
                     /locus_tag="PKN31_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99782.1"
                     /translation="MKKILLVSLVILLVGMLSVCGWGEEKIKIGIVQIIDHPALNNVR
                     DGVIDALLEVYGYIPGENIEYDIQSAQGDMPTANTIAQKFVSEKVDLIVSIATPASQA
                     AVNATREIPVVFSAVTDPVSAGLVSSLEKPGSNVTGVSDMVPVDRQMYLIKFLFPDAK
                     KVGNLYNAGEVNSVVTNDMAKEACAELGMEILEATVATTADVSMAARSLVGKVDAIYV
                     ATDNTVISALDTVAKVCEDNKIPLFLADPTTVSQGALLALGFDYYLHGRQTADLVARI
                     LQGESPGEIPVEFARKLKLLFNPRTVEVLGLDEEEFTSKLNAFIEDMKAQGVEVSLEA
                     LPE"
     gene            2829..3722
                     /locus_tag="PKN31_07400"
     CDS             2829..3722
                     /locus_tag="PKN31_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA99783.1"
                     /translation="MQEGLLFGILALGVFITFRCLDFPDLTVDGSYPLGAAVFAVMIY
                     HGFGAGWAMLAAFLAGALAGAFTGFLHTFSRIPALLSGILTMTCLYSINLRIMGRPNI
                     SLSENLGHRTIFVILRDWFPEVPEAYLRLAFLLVLIVGIKLLLDFFLGTEIGLSIRAT
                     GDNETLVESQGVNPDSMKLIGVSVSNALVAFSGAMFAQHQGFVDVNMGIGMVVSGLAS
                     VIVGEVLMRGKTIFVMTLQVIVGSIVYRLATAVALNWGYNIGFKPYDLKLFTGLIVII
                     ILSYPAVQERFSRRARTKQNG"
BASE COUNT         1018 a          731 c          988 g         1069 t
ORIGIN      
        1 atttttagtt gaggcaacga taccatttca ctttgatttt aaatgaagca actcggcccc
       61 ttgacagctc gacctcttgc ttttaaaata aacacggtaa aagaaacttc ttcagggcag
      121 ggtgaggatt aaaccaattc ccgaccggcg gtaaagggca gaagccctga gcccgcgagc
      181 cctccggggt agatccggtg agaagccgga gccgacggta aagtccggaa gaaagaagaa
      241 gtaaagatga gaagcatcaa aagccctgga agttcaagtt tccagggctt tttttgaccc
      301 tggagaagaa aaaggaggca tttttcgtgg aacaaaaaaa gctggtttat ggtggagcat
      361 tggctggatt atctctggtg gtaagtttgt taattcattt tcctcttatt cctcaagcac
      421 cttttcttct ctatgacccg ggggatatcc ccattttggt ggccggtttt aaatttggtc
      481 caggattggg cctattgttg actgctgtgg tggcggtgct atttgcgcta atcaccggtc
      541 aaggaggccc ctggggagtg cttatgcatt ttttagctac tgggagctat gtggctgtgg
      601 ctggttttat atatcaaaga cgacgtaccc aaaacggcgc aataatcagt ttaatagtgg
      661 ctcccctttt tatgactgtg attatggctg gagccaatct gctcgtaact cctctttata
      721 tgggggtggg aagagaagta gtcaagggga tgcttctccc tgctattatc cctttcaatc
      781 ttttgaaggg agttataaat ggagccatta ctcttttggt ttataagaga atcagcaact
      841 ttatagaaga gcctttatat aaaaggggta aagctactac ccgggtccgg tcttgactct
      901 atagaaagga gaagaatttt aaaccgcaat ccgtaagtag agtaccctcc ccagttgtag
      961 cgagcggtga ctcggtgaca gggaattaaa agaggcaggg gattttgccc cagagctctt
     1021 cccacggcgc gagaagctcg ggggtttcct atcttctctg ctaattgtcc ataagtgagt
     1081 gtttttccgt aggggatgag ggcggtggct tttagaactt cccgggtgaa aggggtaagg
     1141 tcatctaaat aaaaaggaca ggaaaaagag acggggtaac cctcaaaata attctgaatc
     1201 gcttcttcca gatagggaaa gggacttttt tcctggggac gaagacaaaa actaaacccg
     1261 gcttttttct gaaagataga taaagctttt tccttgcttt tctggggcaa gataaaagcc
     1321 cggagcctat cttcttgcca ggaaacgaga gaaaagccaa gccaggttct caaataagaa
     1381 aaataagctc ttgacactct ttacttatcc tcacttccga gtataattat agctaaatta
     1441 tataagaaat atagcagaaa aaacaaaaaa taatccgaag aagggaaaaa gtagtttttg
     1501 ttaccaattg cagagagccg ggagaggtgg gagcccggta ttggagcaaa tacgaatggg
     1561 tcctggagga gtaaaccgaa agggataatt ccgattaagg tttaccgttt ccccgataga
     1621 ggaaaagagt ggggttagat acccaaaaag ggtggcaccg cgagctttgc ccctcgtccc
     1681 tgtgtggcga ggggcttttg tttatagagc ctaaaaagga ggtaggttaa ggtgaagaaa
     1741 attcttcttg taagtttggt gatactcctc gtgggaatgc tttcggtttg tggttggggg
     1801 gaagaaaaaa tcaaaatcgg gatagtgcaa ataatagacc accctgctct taataacgtg
     1861 agggatgggg taatagacgc tcttcttgaa gtgtatggct atatcccggg cgaaaacatc
     1921 gagtatgaca ttcagtcggc gcagggggat atgcccaccg ccaataccat tgctcagaaa
     1981 tttgtttctg aaaaagtgga tttaattgtc tccattgcca cacctgcttc tcaggcggca
     2041 gttaatgcca ctcgagagat accggttgtt ttttctgcag taactgaccc ggtgagcgct
     2101 ggcctggtga gcagtttaga aaaaccggga agcaatgtaa ccggagtatc tgacatggtg
     2161 ccggtggata gacagatgta tctcataaaa tttctgttcc ccgatgctaa aaaggtgggc
     2221 aatctctata acgctggaga ggtaaactca gtggttacca atgacatggc taaagaagct
     2281 tgcgctgagc tgggtatgga aattctggaa gccacagtag ctaccactgc cgatgtatcc
     2341 atggctgcga gatccttagt ggggaaagtg gacgctatat atgtggccac tgataataca
     2401 gtgataagcg ctttagatac ggtggctaaa gtttgtgagg ataacaaaat tcctctcttt
     2461 ctggctgacc ccaccacagt aagccaggga gcacttttag ctctgggttt tgattactat
     2521 ctccatggtc ggcaaacagc cgatttggtg gcccggattc ttcaagggga aagcccagga
     2581 gaaataccgg tggagtttgc caggaaatta aaacttttgt ttaatccacg aacggtggaa
     2641 gtactgggtc tggatgagga agaatttaca tctaagctta acgcttttat agaagacatg
     2701 aaggctcagg gagtggaagt ttctctggaa gcattgccag agtgataatc aagaggggag
     2761 aagcaaaata cttctcccct ttaaagcaaa agaggaggta gcctattagc aattttttcc
     2821 tttacagctt gcaagaaggt ttattgtttg ggatattggc tctgggggtg ttcatcactt
     2881 ttcggtgtct tgattttccc gacctcaccg tagacggtag ctatccctta ggggcggcag
     2941 tttttgctgt catgatatat cacggttttg gtgcaggatg ggcaatgtta gctgcttttt
     3001 tagctggagc tttagccggg gctttcaccg gttttttgca cactttttct cgtattcctg
     3061 ctcttctttc cgggatatta accatgacct gtctttattc tataaatctc cgaattatgg
     3121 gaaggccaaa tatctctctc tcggaaaatc tggggcatcg aactattttt gttattttac
     3181 gagattggtt tccggaagtg ccagaagctt atctccgcct ggcgttttta ctggtcttaa
     3241 ttgtgggtat caagctctta ctggactttt ttttaggaac agaaatcggc ttgtctatta
     3301 gggccacagg agataacgaa actctggtag agtcgcaggg tgtgaaccct gactctatga
     3361 agctaatcgg ggttagtgtc tccaatgctc tcgtagcctt ttcgggagcc atgtttgctc
     3421 aacatcaagg gtttgtggat gtgaatatgg gtattggcat ggtagtttcg ggcctggcct
     3481 cggtgattgt gggagaggtg ttgatgcggg gaaagacaat ttttgttatg actctccagg
     3541 taatagtggg ctcgatagtt tatcggttag ctactgctgt agctctcaac tggggttata
     3601 acatcgggtt taagccctat gacctgaagc tatttaccgg ccttatagtg attattattc
     3661 tttcttaccc tgctgtgcaa gaaagatttt cccggagggc gaggacaaaa caaaatggat
     3721 aacagagaaa ataaggtaat gctacaactg gaaaatgtga ataaatattt tttccgttac
     3781 actcaggatg agcaatgggc agttcg
//
LOCUS       DAOPSS010000143         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_20051_length_3686_cov_9.566511, whole genome shotgun sequence.
ACCESSION   DAOPSS010000143 DAOPSS010000000
VERSION     DAOPSS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20051_length_3686_cov_9.566511"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /gene="larE"
                     /locus_tag="PKN31_07405"
     CDS             <1..597
                     /gene="larE"
                     /locus_tag="PKN31_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOA99784.1"
                     /translation="LPLKVIEISGEDEPLIWRNTKERCYFCKKRMMRELLALAQKEGY
                     AWVVDGTNQDDEGDFRPGFRATQELGIRHPLLEVGFTKYEIREVSRELHLPTWDKPAS
                     SCLATRIPYGVEITLPLLHRIEQGEELLHNLGFSLSRLRHHGEVARLEISPEEFPLLL
                     EGELRQKVVSGLQALGYTFITLDLEGYHSGSMNRLRSD"
     gene            complement(594..1646)
                     /gene="ltaE"
                     /locus_tag="PKN31_07410"
     CDS             complement(594..1646)
                     /gene="ltaE"
                     /locus_tag="PKN31_07410"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOA99785.1"
                     /translation="MATSHPRWIDLRSDTVTIPTQEMLEAMVQAEVGDDVYGDDPTVN
                     LLQEKAAEKMGKEAALFVPSGTFGNQLAIFTHTQSGDEVLIPESNHILEHEVGAPAVI
                     SRVGLRPLPDQYGQVDIKDLEKRYREEDLHHPRTGLICMENAHSSGKVVPLSNLQEVY
                     QFAQEKGIPVHLDGARIFNAATFLKVKPAQIALYCDSVMFCVSKGLCAPIGSLLVGTR
                     SFIEKARKNRKLLGGGMRQVGYLAAAGLIALEKMTKRLQEDHENARYLAKRLQEIEGI
                     KVAEDRLDINMVFFTVEIERFSPPEFIQFLEEKNIKINLPREGEFRLVTHYWIKKEDI
                     DYFIETGKTYFSRYYT"
     gene            complement(1649..3589)
                     /locus_tag="PKN31_07415"
     CDS             complement(1649..3589)
                     /locus_tag="PKN31_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOA99786.1"
                     /translation="MKEEPERVLFSNGKNGGKKARSLGPVVDLEEHVPPDWWKRIFNS
                     LYLKTDGDVVEDQNITRQEVDYITTILHLSPGDRILDLCCGQGRHSLELARRGFTRVK
                     GLDRSHYLIQKARNQARREGLSVQFKEGDARKLPYPTDSFDVVLILGNSFGYFESIQD
                     DLKVLTEVFRVLKPGGKIFIDVADGEYLRENFQPHSWEWIGKKQFVLRERSLSKDRQR
                     LISREIITHIENGVMADQFYAERLYSVTELISLLEKSGFSRLTTHGEHVPSSQRNQDL
                     GMMEKRILLSGEAKKEWTHPKRIKGATLKVVVLLGDPGQPDPLKPQTVFDEDDIYTVN
                     QLKAALGELKGYQFTYLDQHRTLLHDLLRLRGKVDLVFNLCDEGFNNDARQELHVPAL
                     LDILGLPYTGSGPQCLAYCYDKSLVRGIAKELNIPVPRAFLIKPEDFTFKLPFDFPVL
                     LKPNFGDSSFGITEKSVATNLDDFVEAVSEIRNKFGYDKPILVEEFLPGKDLSLGIIG
                     NPPESYQVLPITEEDYSALPPGLPPICGYEAKWLPDSPYGKVKSLPSKLPEETEKIIV
                     ESSIKLFERLECRDYARFDWRLDREGQPRLLEVNPNPGWCWDGHLAKMAHFAGISYHQ
                     MLEAVLEAAICRLNIVALKDKP"
BASE COUNT          971 a          812 c          819 g         1084 t
ORIGIN      
        1 cttcctctta aggtcataga aatttcggga gaagatgagc ccctcatctg gcgcaatacc
       61 aaggaacgct gctatttctg taaaaaaagg atgatgcggg aactgttagc tctggcacaa
      121 aaagaaggct acgcttgggt agtagatgga accaatcaag atgacgaggg ggatttccgt
      181 cctggttttc gggctaccca ggagttggga atcagacatc cccttttaga ggtaggattt
      241 acgaaatatg aaatccggga ggtttctcga gagcttcacc ttcccacctg ggataagcct
      301 gcctcttcat gtctggctac gcgcattcct tatggggtgg aaataaccct tcctctactg
      361 catcgaattg aacaaggaga agaactttta cataacctgg gcttttctct ctcgcgcctg
      421 aggcatcacg gtgaggtggc tcgtttagaa atatcgccag aagaatttcc tctcctttta
      481 gaaggggagc tgaggcaaaa agtagtttct ggcctgcaag ctctgggcta cacttttatt
      541 actctggact tagaagggta tcattccggg agcatgaata gacttagaag cgattaagtg
      601 tagtatcggg agaaataagt tttgccagtc tctataaagt aatcaatatc ctctttcttt
      661 atccagtagt gggtaaccag gcgaaattct ccctcccggg gaagattaat ttttatgttt
      721 ttctcctcta aaaactggat aaattctggt ggggaaaagc gttctatctc tacggtgaaa
      781 aacaccatat tgatatccag gcggtcttct gctactttta ttccctcaat ttcttgtagt
      841 ctcttggcta agtaacgggc gttttcatgg tcttcttgta agcgcttggt cattttctcc
      901 agagctatca atccggcagc cgctaaatat cctacctgcc gcatacctcc tcctaaaagt
      961 ttacgatttt tgcgggcttt ctcaatgaaa gagcgagttc ccacgagcag agaaccaatg
     1021 ggagcacaga gtcctttaga aacacagaac atcactgagt cgcaataaag ggcgatttgt
     1081 gctggtttta cttttaaaaa agtggcagcg ttaaaaatcc tcgctccatc taagtgcaca
     1141 ggaattccct tttcttgggc aaattgataa acttcctgga gattagaaag aggaactact
     1201 ttaccagacg agtgggcatt ttccatacag attagccctg ttcgagggtg gtgaaggtct
     1261 tcttctcgat agcgtttttc taaatctttt atatctacct gtccatattg gtcaggaaga
     1321 gggcggagcc ccacccggga tattaccgct ggggctccca cttcgtgctc taaaatgtgg
     1381 ttactttcgg ggattaatac ctcgtctccg ctttgagtgt gagtgaatat agctaactga
     1441 ttaccaaaag ttcctgaagg gacaaaaaga gccgcttctt ttcccatttt ttctgctgct
     1501 ttttcttgca acaggtttac tgtggggtca tcaccataaa catcatctcc tacttctgcc
     1561 tgtaccatag cttccagcat ttcctgagta gggatagtga cagtatcgct acggaggtca
     1621 atccatctgg gatgagaagt agccattttc atggtttatc tttcaaggcc acaatattga
     1681 ggcgacagat agctgcttct aagacagctt ctaacatctg gtggtaagaa atcccggcaa
     1741 aatgagccat tttagctaag tgcccatccc agcaccatcc ggggttggga ttgacctcta
     1801 atagccgggg ctgtccctct ctatccagtc gccaatcaaa acgggcatag tctcgacact
     1861 ctaagcgctc aaacaactta atagagcttt ccacaattat tttctcggtc tcctccggta
     1921 gtttactggg taaggatttt actttaccat agggagagtc aggtagccac ttggcttcgt
     1981 aaccacaaat agggggaagc ccgggaggaa gagcactata atcttcctcg gtaatgggta
     2041 gcacctgata ggattcagga gggttaccga taatccctaa actcaaatct ttgccaggga
     2101 gaaattcttc tactaagatg ggtttatcgt agccaaattt gtttcttatt tccgataccg
     2161 cttctacaaa atcgtctaag ttggtggcta cacttttctc ggttatacca aagctggagt
     2221 cgccgaaatt gggcttaagg agaacgggaa aatcaaaggg caatttaaaa gtgaaatctt
     2281 ccggtttaat caaaaaagcg cgagggacag gaatattcag ctctttagct attccccgaa
     2341 ctaaagactt atcatagcaa taagctaaac attgagggcc agaaccagtg tagggtagtc
     2401 ccagtatatc taaaagggca ggaacgtgga gttcttgtcg ggcatcgtta ttaaatccct
     2461 cgtcgcaaag gttgaagacc aggtctactt ttcctcgcag tcgtagtaag tcgtggagta
     2521 aagtgcgatg ctggtctaag taagtaaatt ggtagccttt caattctcct aaagcagctt
     2581 taagctgatt tacagtatag atatcatctt cgtcaaaaac ggtttgaggt ttaagagggt
     2641 caggttgtcc ggggtcaccc agcagcacta ctacttttaa agtagctcct tttattcttt
     2701 tggggtgtgt ccattctttt ttggcttcgc cactcaggag aattcgtttt tccatcattc
     2761 ccaggtcctg gttacgctga gaagaaggaa catgctctcc gtgggtggta aggcggctga
     2821 acccggattt ctctaaaaga gatattaatt ctgtaactga atatagtctt tcggcataga
     2881 attgatctgc cattactcca ttttcaatgt gagttattat ttcccgagaa atcaagcgtt
     2941 gtcgatcttt agacagagat cgctcccgca atacgaattg ttttttacca atccattccc
     3001 aggagtgagg ttgaaaattt tctctcaggt attcaccgtc ggccacgtct ataaatattt
     3061 ttcctccggg tttaagaact ctgaaaacct ctgtcaatac cttgaggtca tcttgaatgc
     3121 tttcaaaata tccaaagcta tttcctaaaa tcaataccac gtcaaagcta tcagtggggt
     3181 agggtagttt gcgggcatct ccttctttaa actgaacact caacccctct cgtcgggcct
     3241 gattcctggc tttttggatt aagtagtgag agcggtctaa accttttacc cgggtgaatc
     3301 cccgccgggc taattccaga gaatgtcttc cctgtccgca acagaggtct aaaatccggt
     3361 ctccgggaga gagatgaaga atagtggtga tataatccac ttcttgtctc gtgatgttct
     3421 ggtcttctac tacgtctcca tcggttttta agtaaagaga gttaaaaatt ctcttccacc
     3481 aatcgggtgg gacgtgttct tccaaatcta caactggccc taatgacctg gcctttttgc
     3541 ccccgttttt accgttagaa aatagaactc gttcaggctc ctctttcatt ttcctccacc
     3601 ttacctggga gattttttat tatgaaacga ttatagaagg atggtgacaa aaagcaatac
     3661 cggagaaaaa ttatttggga aaattt
//
LOCUS       DAOPSS010000144         3559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_20996_length_3559_cov_8.505422, whole genome shotgun sequence.
ACCESSION   DAOPSS010000144 DAOPSS010000000
VERSION     DAOPSS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20996_length_3559_cov_8.505422"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /gene="metG"
                     /locus_tag="PKN31_07420"
     CDS             complement(<1..1294)
                     /gene="metG"
                     /locus_tag="PKN31_07420"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOA99787.1"
                     /translation="MDNSFYVTTPIYYVNDVPHIGHAYTTCAADVLARYYRLRGYQVL
                     FSTGTDEHGQKIEKAARDLGITPQELVDKMVIRFQDLWKKMNISYDEFIRTTDPRHIK
                     VVQDFFRLLDERGYIYLGDYEGWYCVPCETFWPESQLDERLVCPDCGRSLEKLKEESY
                     FFALSRFQEPLLQYLESHPDFIMPESRHHEIESFVRGGLKDQSISRLGLEWGIPVPSD
                     PRHTLYVWFDALVNYLTVAGWGTSEEKFSNFWPYAYHLVGKDILRFHAVLWPGMLMAA
                     SLPLPRRIFAHGWWTVEGEKMSKSRGNVVDPYLMIDRYGVDRFRYFLMREVSFGLDGD
                     FSEKALIERSNADLSDNFGNFVHRTLGMVWRYKKGVVKKPSPYQDGEWEKLLEEVKEG
                     FQYYMERFAFSQALEQVWKLVHFANRYIEREAPWKLHKE"
     gene            complement(1315..1848)
                     /locus_tag="PKN31_07425"
     CDS             complement(1315..1848)
                     /locus_tag="PKN31_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOA99788.1"
                     /translation="MAEDLSPREREEVLEFLYQCLMGKRIFVFSTTPQFGRKCLETNP
                     EGMVITGHYSVSEGRLARVAAEYVGGCGAGRAYCALQPDGVITPCVFMPIVLGNIIEQ
                     PFFQIWRESSVLKELRDRTLYRGHCGECDYQLVCGGCRARAYTYFEDYLAPDAGCKFN
                     QEFWEMIEKEEPYLANF"
     gene            2356..3429
                     /locus_tag="PKN31_07430"
     CDS             2356..3429
                     /locus_tag="PKN31_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOA99789.1"
                     /translation="MLKPKLMLAISLLIAVSIIVFSIPQVTLANQPIKLSVNGEEINT
                     DILPQIINGRVMVPARWVAEALGAKVHWGEETKTVEISTQNWLLQLEKILHQAEEEFA
                     LAPDAKGEIKRLWRVGDNYILLENVWYRDYDYWLCNTYTGQKNWIVSFIENARVEKIE
                     DNEIIFIAKGGGDTGDYDFPYLLRYNLEEKELSRTQMYLQRDVAFGAVSSWSLLLEKV
                     SLQGEDIILNLKVAPDQILAGGFKKPLTIVDYQRDSISMRIYGVTSKKYDNQVIEPDH
                     HLIKKVEWRALSPDEPVKNPELLQMDFPYGAALENINFNRPSIEVKIYTPEKVAYNVS
                     TKIEDDNLIYTIKFNPPVNSHSR"
BASE COUNT         1085 a          830 c          637 g         1007 t
ORIGIN      
        1 cttctttgtg caatttccag ggagcttctc gctcgatgta gcggttagca aaatgtacca
       61 gtttccagac ttgttctaaa gcctgagaga aggcaaaccg ctccatatag tactggaaac
      121 cctcttttac ctcttccagt aacttttccc attctccatc ctgataggga gaaggctttt
      181 tcaccacccc ttttttatat ctccatacca tccctaaagt tcggtgaaca aaattaccaa
      241 agttatcaga taaatcagca ttagaacgtt ctatcagcgc tttttctgaa aagtctccat
      301 ccagaccaaa gctcacttct ctcatcagaa agtatcggaa acggtctacc ccatagcggt
      361 caatcatcaa ataaggatct accacgttac ctcgagattt agacattttt tctccctcta
      421 ccgtccacca accatgggcg aatatccggc gagggagagg taaagatgca gccatgagca
      481 tcccgggcca gagaacagca tgaaagcgaa gaatatcttt acccaccaga tgataggcat
      541 aaggccagaa attagagaat ttctcttccg aagttcccca gcccgctacc gttaaataat
      601 taaccaaagc atcgaaccat acataaagag tatgacgagg atcggaagga accggtatcc
      661 cccactccag ccccagacga gagatgctct ggtctttaag cccccctctt acaaaacttt
      721 caatttcgtg atgccggctt tcgggcatta taaaatcggg atggctttcc aaatattgta
      781 aaagtggctc ctggaagcgg gaaagagcaa aaaaatagct ctcttctttt aatttctcta
      841 aagacctccc gcaatcggga caaaccaagc gctcatccag ctggctttcc ggccagaaag
      901 tttcacaggg gacacaatac catccttcat agtctccgag ataaatgtag ccccgctcat
      961 ctaataagcg gaaaaaatct tgcaccacct ttatgtggcg agggtcagtg gtacggataa
     1021 actcatcata agagatattc attttcttcc acaaatcctg gaaacgaatc accattttat
     1081 ctaccagctc ttgaggggtg attcccaaat cccgagcggc tttttctatc ttttgtccgt
     1141 gttcgtcggt accggtagag aaaaggactt gatacccccg caagcgataa tagcgagcca
     1201 gaacatcagc agcacaagta gtgtaagcat gaccgatatg aggaacgtca tttacatagt
     1261 aaataggagt agtaacgtaa aaactgttat ccatctttca cctcttcaat caaattaaaa
     1321 atttgccaga taaggctctt ctttttctat catttcccaa aattcttgat tgaacttaca
     1381 acccgcatca ggagctaaat aatcctcaaa ataagtataa gcccgagctc gacatcctcc
     1441 acaaaccagc tgataatcac actctccgca atgtccccga taaagggttc tatctctaag
     1501 ttcttttaac actgatgact ctctccagat ctggaagaaa ggttgttcta taatgtttcc
     1561 taaaacaata ggcatgaaca cacaaggagt aattacccca tcaggttgta gagcacaata
     1621 agctctacct gctccgcatc ctcccacata ttccgctgcc acccgagcca gtctaccctc
     1681 cgataccgaa taatgaccag taatgaccat tccttccgga ttcgtctcca aacatttcct
     1741 accaaattga ggggtagtac tgaaaacaaa tatccttttt cccatcaaac actgatagag
     1801 aaattctaac acttcttctc gctcccgggg agaaaggtct tcagccaaaa tttcttgtac
     1861 ccttcccgta atgggtgaga attttcttca gccttactgc tccgtctttt tccggttctc
     1921 ttaatcatca aaatattccc tctgctagtt tagaatcgag ataatgcctt actctttctg
     1981 gattcataaa atgactctat ccattctccc cacctctttg acttatctta tcctatttta
     2041 tacctatttt atcaaggaaa aaaacaaaaa gaactctttt ttgtaaagat ggagcaattt
     2101 tatcggttct cttttttcct gaactttatt tgtaattctt gacgtaaaaa gtggaacagc
     2161 ttaacagccc ctcttttcta cttttcctga agattagcca ctccaccctt taattttcag
     2221 ggtgacatta tcgcagaata agcacattga ggcaactttc ctctttactg gaaatctttc
     2281 cagtgttaag attcaatgac tgaagctaaa atgtaaagtt agtagttcct tattatttcc
     2341 ctgaggagga agtttatgtt gaagccaaaa ctgatgttgg caatatctct tcttatagca
     2401 gtctcaatta ttgtattttc tattcctcaa gtaactttag ctaaccagcc tattaaactt
     2461 tcagttaatg gcgaggagat aaacactgat attctacccc aaattattaa tgggagagtt
     2521 atggttcccg caagatgggt agctgaagcc ctgggagcta aagtacactg gggcgaggaa
     2581 actaaaacag tagaaatttc tacccaaaat tggcttcttc aattagagaa aatacttcac
     2641 caggctgaag aagaatttgc tctagcacct gatgcaaaag gagaaataaa aaggctgtgg
     2701 agagtaggag ataactacat cctattagaa aatgtatggt atagggacta tgattattgg
     2761 ctctgcaata cctatacagg acaaaaaaac tggattgttt cttttataga aaatgcccgg
     2821 gtggaaaaaa ttgaggacaa cgagatcatt ttcattgcta aaggaggcgg cgataccggt
     2881 gactacgatt tcccttatct tctccgttac aatctggaag agaaggaatt atctcgcacg
     2941 cagatgtatt tgcaacgtga tgtagctttc ggagccgtta gctcctggag ccttctcctc
     3001 gagaaagtct ccttgcaagg tgaagatata attctcaact taaaagttgc tcctgaccag
     3061 atattggcag gtgggttcaa aaaacctcta accattgtcg actatcagag agattccatt
     3121 tctatgcgca tttatggcgt tacctccaag aaatacgata atcaggttat tgaacctgac
     3181 caccatttaa ttaaaaaggt agaatggaga gcattatcac ccgatgaacc ggtgaaaaac
     3241 ccggagctac tccagatgga ttttccttat ggtgccgctt tagaaaacat aaactttaat
     3301 cgaccctcca tagaagtaaa aatctatact ccagaaaaag tagcttacaa cgttagcact
     3361 aaaatagagg atgataatct catctatacc attaaattca atccgccggt aaactctcat
     3421 agccggtaaa atcgaatcat aatatcccgc aaaatctgtt atttacaata aggagtaaat
     3481 cctttatcgc ttctcctgtc cgtcatccta aatgtattta tcacccttta ggtgtcactc
     3541 ctgaatgtat ttatcagga
//
LOCUS       DAOPSS010000145         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_21319_length_3514_cov_7.133565, whole genome shotgun sequence.
ACCESSION   DAOPSS010000145 DAOPSS010000000
VERSION     DAOPSS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21319_length_3514_cov_7.133565"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..2254)
                     /locus_tag="PKN31_07435"
     CDS             complement(131..2254)
                     /locus_tag="PKN31_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA99790.1"
                     /translation="MKNLRYPHLFSPIEIGNFVVPNRIVHAPTDISSANADGSVNQRV
                     ITYHEEVAKGGCGFIIVGATTPDKATGRPTVTCVAADEDPMIPGLANLAEAMHRHGAR
                     CAVQIQHPGRQAAWPRKDLFAPTDMVVSLPGSAGHEIVYAEEAGSKGKSIRAMTIEEI
                     YDIIEKFAEAAWRVQQAGFDAVELHGAHGYLIAQFMSPYVNRRNDRFGGSFINRMRFV
                     LEIVDRIHRKCGKDFPVGLRYSGEEYIEGGRTLEESVKVAKLMEESGVAFLDISAGIF
                     EKPAPTMDPMYYPQGWNTYTAEEIKKHVNIPVITTHSLRDPAYCERILAGGKADMVGL
                     SRQMVADPYWANKAKAGETEEIRKCISCLVGCWQESLLVKREMRCAINPAVGDERFIH
                     IQPAQEKYKVAIVGGGPGGMEAARIATLRGHKVTIFEKSNELGGAILYCCAVPGKEKM
                     KWYADWIRRQMNKLGVEVHLQKEVKLEELKAFDAAIIATGGKVTRPDIPGVNLPFVVT
                     FEDVLRCKKRSCEFYTERPEPVECGDTVLVWGDNFGAADTVEKLGFEGKKVFVVTENR
                     EFASWMEPCHRDVMMNRFAGGNGEGLKTRTYAHPVTVITESTVVEIKDQGEVVIQDRE
                     FNKSTIKVDNLVLTQVKCNDDLYQELLDAGIVVAKIGDAKKVRNLRGAVTDGANIGFT
                     LDLDLMMNANNSPIANLPTEVKVSG"
     gene            complement(2278..2979)
                     /gene="deoC"
                     /locus_tag="PKN31_07440"
     CDS             complement(2278..2979)
                     /gene="deoC"
                     /locus_tag="PKN31_07440"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOA99791.1"
                     /translation="MDYSCEDIAKMIDHSLLRPELTDEDIVAGCEVARKYQVAAVCCR
                     PSDVSRVKELLQGSGVKVDAVVGFPHGSHKTKTKVFEAEEAIKDGAEELDMVIHIGKL
                     RSRDFDYVRNDIKAIADVAHSHGVILKVILENCYLDDELKEIGCKLAEEAGADFVKTS
                     TGFSPGGATIEDLKLMRQATSPRVQIKAAGGVRSLDMALKVREAGATRFGATRTADIM
                     EECYQREKQEKKGCC"
     gene            complement(3378..3453)
                     /locus_tag="PKN31_07445"
     tRNA            complement(3378..3453)
                     /locus_tag="PKN31_07445"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3418..3420),aa:Ala,seq:tgc)
BASE COUNT         1009 a          876 c          592 g         1037 t
ORIGIN      
        1 cctccgtgtt tacaggccct ttttggtcaa agtaagttac ttgaatatcc atattacccc
       61 cctcaggata tctctatcca gtcccaacta taagaaatat acttgggact ggatagagaa
      121 agaaaattaa tcagcctgaa actttcacct cagtgggaag attagcaata gggctattat
      181 tggcgttcat cattaaatct aaatccagag taaagccaat atttgcccca tcagtaaccg
      241 cacctctcag gtttctcact tttttggcat ctcctatctt agctactaca atacccgcat
      301 cgagcaattc ctgatacaaa tcgtcgttgc attttacctg agtcaaaacc aaattatcta
      361 ctttgatggt agatttatta aattcgcgat cttgaatcac tacttctccc tggtccttaa
      421 tttccaccac tgtagattca gttattactg tcaccggatg agcataagtc cgggttttaa
      481 gtccttctcc atttcccccg gcaaatctat tcatcattac atcgcgatgg caaggttcca
      541 tccaggaagc aaattctcga ttttcagtta ccacgaagac tttctttccc tcaaaaccta
      601 atttttctac tgtatccgca gctccaaaat tatctcccca gactaaaacc gtatcaccgc
      661 actctaccgg ctccggtctc tcagtataaa attcacagct tctctttttg catcgcaaaa
      721 catcctcaaa ggtaactaca aaaggtagat taacccctgg aatatcaggc cttgttactt
      781 tacctcctgt agctattatt gcagcatcaa aagctttaag ttcttctaat tttacctctt
      841 tttgcaaatg cacctcgaca cccaatttat tcatctgtcg gcgaatccaa tcagcatacc
      901 atttcatctt ctctttgcct ggaaccgcac aacagtaaag aatagctcca cctaattcgt
      961 tactcttttc aaaaatagtt accttatgtc cacgcaaggt agcaatacga gcagcttcca
     1021 tacctcctgg gccaccacct actatggcta ccttatattt ctcttgtgcc ggttgaatat
     1081 gaataaatct ttcatcgcct actgcagggt taatagcaca gcgcatttct cttttgacaa
     1141 gaagcgactc ctgccagcat cccaccaaac aagaaatgca tttcctgatt tcttctgttt
     1201 caccggcttt ggccttatta gcccagtaag gatcagctac catctgacga gaaagcccta
     1261 ccatatcggc tttgcctcca gctaaaattc tttcacaata agccggatct cggagagaat
     1321 gagtagtaat cactggaata ttgacgtgtt ttttaatttc ttcagcagta taagtattcc
     1381 atccctgagg gtagtacata ggatccatag tgggagcggg cttttcaaaa atgccagcac
     1441 tgatatctaa aaaggctact ccgctttcct ccattagctt ggctacttta acgctttctt
     1501 ctaaagttct tcctccttcg atatattctt ctccagaata gcgaagtcca acaggaaaat
     1561 ccttcccaca tttcctgtga atacgatcta caatttctaa aacaaaacgc atacggttta
     1621 taaagctgcc tccaaaacgg tcatttctcc tattaacata agggctcata aactgagcta
     1681 ttaaataacc atgagctcca tgaagctcta cggcatcaaa acctgcttgt tgaactctcc
     1741 aggccgcttc ggcaaatttt tcgatgatat cgtatatctc ctcaatggtc atagcccgga
     1801 tcgactttcc cttagaacct gcctcttcgg catatacgat ttcatgacca gctgaaccag
     1861 gaagagaaac caccatatca gtaggagcaa aaagatcttt gcgaggccag gctgcctgcc
     1921 gcccaggatg ttgaatttgt actgcacaac gtgctccgtg acgatgcatg gcttcagcta
     1981 aattagctaa tcccggaatc atagggtctt catcggcagc cacacatgta accgtaggac
     2041 gtccagtagc tttatctgga gtagtagctc ctactattat gaaaccacat ccccctttag
     2101 ctacctcctc gtgataggtt ataacccgct ggtttactga cccatcagca ttagctgagc
     2161 ttatatcagt aggagcatga acgatacggt tgggaaccac aaaattacca atttcaatgg
     2221 ggctaaaaag atgaggataa cgaagatttt tcattcagat aaccttcctt tctattttta
     2281 gcaacatcct tttttctctt gtttttctct ttgataacat tcttccatta tatccgccgt
     2341 tcttgttgct ccaaagcgag tagcacccgc ttcccttacc ttgagagcca tatccaggct
     2401 tcttactcct cctgctgctt ttatctgcac tcgaggagaa gtagcctgac gcatgagttt
     2461 aagatcttcg atagtagcac caccggggga aaatccagta gaagttttta caaaatcagc
     2521 tcctgcttct tcggctaatt tacagcctat ttcttttagc tcatcatcca gataacaatt
     2581 ctccaaaata acttttaata tcactccatg gctatgagcc acatcggcta tcgctttaat
     2641 atcatttctg acatagtcga aatctcgact gcgaagcttc ccaatatgaa ttaccatatc
     2701 caattcttct gccccatctt tgatggcttc ttcagcttca aacactttgg tctttgtctt
     2761 atgcgaacca tggggaaacc ctactacagc atctactttc accccactac cttgaagcaa
     2821 ctccttcacc cgagatacat cagaaggccg acaacatact gctgctacct ggtatttcct
     2881 ggctacttcg caccctgcta caatatcttc atcagttaac tccgggcgta gtagcgagtg
     2941 gtcaatcatt ttagcaatat cttcacaaga ataatccatt ccacaccccc tataatgtta
     3001 taacaatatc tcaacctatc ttataccact actttcctca ttgtcaagga taaatgggct
     3061 ctataacaaa gtgtggaaaa aatacgcgaa aaaaagtatg gtagcaaaag caagtgagaa
     3121 attaagagga agaagagctt gccatcatag gaaaagatag aaattagcat cacaatttta
     3181 ttgagagtta aaaagatttt taaagattca aagccagaaa agtttactga ttctggtcta
     3241 acttcagagg aaaggaatta aaaaatatct accttaggga ttaactataa aacctttttt
     3301 aattagcaac ggcttcaggg gttagaaaaa gttaaagagg aaacacaaaa gtggctaaaa
     3361 ttagtttgtg gagcttctgg tggagatgac gggattcgaa cccgtggcct ccggcttgca
     3421 aagccggcgc tctcccagct gagctacatc cccacaaaca agaaaatatt ccgcctattt
     3481 ccttttagtc tttctactcc ttagaaagga ggtg
//
LOCUS       DAOPSS010000146         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_21649_length_3472_cov_7.240562, whole genome shotgun sequence.
ACCESSION   DAOPSS010000146 DAOPSS010000000
VERSION     DAOPSS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21649_length_3472_cov_7.240562"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..886)
                     /locus_tag="PKN31_07450"
     CDS             complement(23..886)
                     /locus_tag="PKN31_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA99792.1"
                     /translation="MFKFGVDTFIWSEAFSEKDLWIIPKAKELGFEVLDISISHPESF
                     PTSLVKKKVEEVGIEVVTTTTLGEETNLISPDSDIRAQGIKHMKLLIDINVELGSKIT
                     GGVNYAAWGYLPGRPRTEEEWDWSIDAMREIAEYARERSDLIIAVEPVNRFETHFLNV
                     AEDAVRYCNDVGTGNVKVHLDSFHMIREETSFRGAVETCGKEYLGYVHVCENNRGIPG
                     TGLVPWREFFQALKDIGYNGPLVIESFDPSFEELNRQCAIWRKLAESGEELAVKGLQN
                     LKAIAQEIEKE"
     gene            1098..2279
                     /locus_tag="PKN31_07455"
     CDS             1098..2279
                     /locus_tag="PKN31_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA99793.1"
                     /translation="MNKEVSQRKARALRFIVLMGLVSLFGDIVYEGARGVTGPYLALL
                     GASAFAVGLVGGLGEFIGYALRLLSGFIADRTKAYWFFTFIGYGLIFSIPLLSFANSW
                     WVAALFIIMERAGKAFRSPARDTILSYATKEVGRGFGFGIHEALDQIGAIVGPLIFSF
                     IIFFGGDYRAGFSILWIPALIVIVILVIARNQLPHPEKLETSEEPEEKDSKIFSRIFI
                     LYSLFTFLSVAGFANFPLISYHLKIKSIMSDVQIPTFYALAMGVDAIVAILIGKAYDR
                     VGLKTLMVIPLLSLFIPFLAFSNSIASVIIGVILWGGVLGVHETIMRAAIADLTPISR
                     RGSAYGIFNTIYGLSWLLGGALMGILYEISLSYLIILAALFELASIPVLLVLEREQKT
                     K"
     gene            2357..3394
                     /locus_tag="PKN31_07460"
     CDS             2357..3394
                     /locus_tag="PKN31_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOA99794.1"
                     /translation="MRALFGKAPFKLEWRDLPVPDLQPGKILVRVEAGGICGTDIHFL
                     RYNEEWTPLGHEAVGIVEKASDGVEGVQVGDRVIIENHAACWRCKQCKNGRPQYCENL
                     TTYMSDQAGFSDYLVVRYDMVHHYDPKQLELTHACLAEPLTVALDVTLRAGLELNDEV
                     VVFGPGPIGLMAVKIAKLQGARRVFLVGSNRSTSRGAYRLEIGHKMGADVILSYEENN
                     VVETILAAVPTGVDRVIVTAPPRTIKDAIQISRFGGIIAFNGIEFGAGSQVTFDANEF
                     HFKKLQLRASHAIPNHYFPMAIDLLVQRAIDPTLLLTHVFTLDEAEKAFQTLTNPEEK
                     AIKVVLKQSAR"
BASE COUNT          947 a          784 c          686 g         1055 t
ORIGIN      
        1 acaacaggat ataaaactga aactattctt tctcaatttc ttgagcaata gcctttaagt
       61 tttgtaatcc ttttacggcc agttcttctc ctgattcagc taatttcctc cagatggcac
      121 actgccggtt tagctcttca aaactggggt caaaggattc aataactaaa ggtccattgt
      181 atccaatatc ttttagagcc tgaaaaaatt ctctccaggg taccagtccc gtccccggta
      241 ttcctcgatt attttcacac acatgaacat atcccagata ctctttccca caagtttcta
      301 ctgctcccct gaagctcgtc tcttcccgaa tcatgtggaa agaatccaga tgcactttca
      361 cattccccgt tcccacgtcg ttacagtagc gcactgcatc ttcagcaaca tttaaaaaat
      421 gggtctcaaa ccggttaact ggctctacag caataatgag gtcacttctc tccctggcat
      481 attccgctat ttctcgcatg gcatcaatag accaatccca ttcttcttcc gttcttggtc
      541 ttcctggtaa atacccccag gcagcgtagt ttactccccc ggttatttta gagcctaact
      601 ccacgttgat atctatcaaa agtttcatat gtttaatccc ttgagccctg atatccgagt
      661 caggagaaat aaggttggtt tcctcaccca gggtggtggt agtcacaact tctatcccca
      721 cttcctctac tttctttttc actaaacttg tggggaagct ctctggatga ctgatagata
      781 tgtccaaaac ttcaaacccc aattcttttg ctttgggaat aatccacaga tctttttctg
      841 aaaaagcttc cgaccagata aaagtatcta ctccaaactt aaacatttaa actgcctccc
      901 cctgtcattt tttaaataag ttcactttta tttttaattt tttatcatca ttgttatttt
      961 atcatcttta gatgttatta aaaataaccc ccaagtaatt acgctaatta ctaaaataat
     1021 cgtaacatac ggtcatcttg aaaagacatc agaccagatt tataatacca ccaccagtaa
     1081 taggaggagg gaaaaagatg aataaggaag tctctcaaag aaaagccaga gccctacgat
     1141 ttatagtcct catgggattg gtaagtctgt tcggtgacat agtttacgaa ggagcaagag
     1201 gggttaccgg tccttattta gctcttttag gagcgagtgc atttgcggta ggtttagtcg
     1261 ggggtctggg tgaattcatc ggatatgctc tccgacttct ttccggtttt atagctgacc
     1321 gaactaaggc ctactggttc ttcaccttca ttggttatgg actcattttc tctatccctc
     1381 tcctttcttt tgctaatagc tggtgggtag cagccctatt tataattatg gaaagagcag
     1441 gcaaggcttt ccgctctcca gcccgagata ccattctctc ctacgctacc aaagaagtgg
     1501 ggcgaggatt tggctttggc atccatgagg cgttagacca gattggagct attgtaggcc
     1561 ccctaatttt ctcttttatt atattctttg gtggcgatta tcgtgcaggg ttttccattc
     1621 tctggatacc agccctaatt gttatagtta ttctggtcat cgccagaaac cagctacctc
     1681 atcctgagaa actggaaact tctgaagaac cagaagagaa agacagtaaa atattttcca
     1741 ggatatttat tctctattct ctttttactt ttctctccgt agcaggattt gctaacttcc
     1801 cgctcatttc ttaccacctc aaaataaagt caatcatgag tgatgtccag attcccactt
     1861 tttatgccct ggctatggga gttgatgcta tagtagctat tttaatagga aaagcctatg
     1921 accgagtggg actaaaaact ctgatggtta tccctttact ttccctcttt atcccctttt
     1981 tggctttttc caatagtatt gcttccgtaa tcatcggagt gattttgtgg ggaggagttt
     2041 taggtgttca tgaaaccata atgcgagcag cgatagctga cctgactcct ataagccgta
     2101 gaggttcagc ttatggaatt ttcaatacca tctacggatt atcatggctc ctcggaggag
     2161 ctttgatggg aatactttac gaaatctctc tctcttattt aattattctg gctgcgctat
     2221 tcgagttagc ctctattcct gttttgctgg ttttagaaag agaacaaaaa accaaataaa
     2281 ttaaaattcg ttatttcaaa ctctttcaca tttgttataa tgaccttata aatacaaagg
     2341 atattggagg aaatccatgc gagcactttt tggaaaagca cctttcaagc ttgaatggcg
     2401 ggatttaccc gtacctgact tgcaacccgg taaaatacta gtgcgagtgg aagcaggagg
     2461 tatatgtggt accgacatac attttctccg gtacaatgaa gaatggactc cactgggtca
     2521 tgaggcagtg ggaattgttg aaaaagcaag cgatggagta gaaggagttc aagttggcga
     2581 ccgggtaatc attgagaatc atgctgcttg ttggagatgt aagcagtgca aaaatggacg
     2641 ccctcaatac tgtgagaatt tgaccaccta tatgtcagac caagccggct tctccgacta
     2701 tttggttgtc cggtacgaca tggtgcatca ttacgaccca aaacagctgg agttaacaca
     2761 cgcttgtctt gctgagcccc tcaccgtagc tttagatgtg acattaagag ccggccttga
     2821 gctcaatgat gaggtagttg tttttggtcc aggccccatc ggattaatgg ctgttaaaat
     2881 tgccaaactg caaggtgctc gtcgagtctt tctcgtgggt tccaatcgct ctacttcccg
     2941 aggagcttat cgtttagaaa taggccacaa aatgggtgct gatgtaattc taagttatga
     3001 agaaaataac gtggtagaaa caatattggc tgctgtgcct acaggagttg atcgagtgat
     3061 tgtgaccgca cctccccgga caatcaaaga cgctattcaa atatcccgct tcgggggcat
     3121 cattgccttc aatggtatcg aatttggagc aggaagccaa gttacttttg acgccaacga
     3181 attccacttc aaaaaacttc aactacgggc ttcccatgct atccccaacc actattttcc
     3241 tatggctatc gaccttttgg tccaacgcgc tatcgacccg acattactgc taacccatgt
     3301 ttttacctta gacgaagcag aaaaagcctt ccaaacttta acaaatccgg aggagaaagc
     3361 catcaaagtg gtacttaaac aatctgcaag gtaaattaaa aaatgaaaac tgctgagaca
     3421 ggagccacag gtactttact ggaagaagag cttggcttcc aggtgtataa at
//
LOCUS       DAOPSS010000147         3462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_21715_length_3462_cov_8.245377, whole genome shotgun sequence.
ACCESSION   DAOPSS010000147 DAOPSS010000000
VERSION     DAOPSS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21715_length_3462_cov_8.245377"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..569
                     /gene="ruvC"
                     /locus_tag="PKN31_07465"
     CDS             57..569
                     /gene="ruvC"
                     /locus_tag="PKN31_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOA99795.1"
                     /translation="MPESAHILGVDPGTAITGFGVIESSARGNSLKVVDYGFIATDKG
                     LPASTRLKFIFEDLKKVIEKFAPQEIAVEELFFNKNSKTAIAVGEARGIVLLCAALYQ
                     VPVYEYTPLEVKQSIVGYGRATKNQVIYMVRQILNIEEEINPDDVADALAVAICHAFR
                     SGCRGNVRLS"
     gene            553..1149
                     /locus_tag="PKN31_07470"
     CDS             553..1149
                     /locus_tag="PKN31_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOA99796.1"
                     /translation="MLDYLKGKVVETGEEDWVVEANGFGFRLKVTPFVFPGDIGEERC
                     LLLRVFMRENGEFTLYGFDEEEERSVFDQLQEVRGVGHRTAFKILSRVKWEDLIELTL
                     NEDVSTLETRTNLSSKTIKKLVLELKPRFLKKGWKIEGKERLGGTVEEVREVLSRLGY
                     RPVEIEGVIDDLWNELVKEGADVETWVREALVKLGGGV"
     gene            1146..2117
                     /gene="ruvB"
                     /locus_tag="PKN31_07475"
     CDS             1146..2117
                     /gene="ruvB"
                     /locus_tag="PKN31_07475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOA99797.1"
                     /translation="MKRESVDDFLIRPQSLADFVGQEKVKSNLQVYIQAARSRGEPLD
                     HVLLFGPPGLGKTTLANIIAHEMNTSLRYLSGPTFTRGGDLASVFSTLSAGEVVFIDE
                     IHRLPPPCEEILYGAMEDFALHLVVGKGPGARSIRIQLPLFTLVGATTRVSLLSSPLR
                     NRFGIVEKLDFYQEEEISKIVKRSAQVMGVEIEPGGAEEIAHRSRGTPRIANRLLRRV
                     RDFAQFFGREIIDEEIAKEAFNHLGVDEVGLTDFDRKFLEVLVKHYQGGPVGIENIAS
                     ALGEDPATLEEVCEPFLLKLGFIARTRRGRIITARGRQYLQKVKEIG"
     gene            2110..2676
                     /locus_tag="PKN31_07480"
     CDS             2110..2676
                     /locus_tag="PKN31_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOA99798.1"
                     /translation="MGDKILLHTCCAPCATYVNEALEKEGWEVVNIFYNPNIHPFDEY
                     ARRYETLVLYLEREGKVLKAPFPYNPLEYFSLITEAENRCPKCYFLRLRKVAQWGKER
                     GFEFFTTTLTISPYQDLSAIWEVGKQIEEELGIKFLVRDFREGFRQSVTRSRELKLYR
                     QNYCGCIFSKYEGVKRRLWKRLTGLKFS"
     gene            2643..2873
                     /locus_tag="PKN31_07485"
     CDS             2643..2873
                     /locus_tag="PKN31_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOA99799.1"
                     /translation="METSYWAKIFLILGLVFIVVGVLLLIVPRIPGINKLGRLPGDIV
                     YRRGNFVFYFPLTTCIIISVVLSLLFALFFRR"
     gene            2883..>3462
                     /locus_tag="PKN31_07490"
     CDS             2883..>3462
                     /locus_tag="PKN31_07490"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOA99800.1"
                     /translation="MRKRVILLIFLVLFVCMNGAWGWGKELEVKVNLNKAKEAVFSSS
                     GGLKIEIGKRYFSVPSSYPVHFREKGGKVEWEEENILSSEVYIYPKGKEPILWGKRPY
                     RGSFIVKASSDGILVINQLPVEDYIKGTMKLELSPAWPVEALKAQIIVSRTYALKNLR
                     RHEEEGFNFCATDHCQRYGGINAEDPVTNRLVE"
BASE COUNT          946 a          607 c          904 g         1005 t
ORIGIN      
        1 gaagtttatt ctaactttga aatagcggat gagattttag aatcactgag tagctaatgc
       61 ctgagagtgc tcatatatta ggagtagatc cgggaaccgc tatcaccggt tttggggtta
      121 tagagtcttc tgctcggggt aattccctta aagtggtaga ttatggcttt attgccactg
      181 ataaaggact ccccgccagt acaagattaa agttcatttt tgaggattta aagaaagtaa
      241 tcgagaagtt tgcccctcag gagatagcgg tggaagaatt gttttttaat aagaattcca
      301 aaactgctat tgctgtggga gaagcacggg ggatagtttt attatgtgct gctctttatc
      361 aggtgccggt ctatgagtat acaccacttg aagtcaaaca atccatagtg ggttacggaa
      421 gagccactaa aaatcaggtt atttatatgg tgaggcaaat tttaaatata gaagaagaaa
      481 ttaatccgga tgacgtggct gatgccctgg cagtggctat ttgtcacgcc ttccgttcag
      541 gatgtagagg aaatgttaga ttatcttaaa ggaaaagtgg tggaaacagg agaagaagat
      601 tgggtggtag aggctaacgg ttttggtttc cgcctgaaag tcactccttt cgtttttccc
      661 ggggatattg gagaagaaag atgtcttctt ttgcgcgttt ttatgaggga gaacggagaa
      721 ttcacccttt atggatttga tgaagaggag gagaggtcag tttttgacca gctgcaggaa
      781 gtcagaggtg ttggtcaccg tactgctttt aaaatcctct ctcgggtaaa atgggaagac
      841 ttaattgagt taactttgaa tgaagatgtt tcaactttag agaccagaac caacctcagc
      901 tccaagacca taaagaagct ggtgctggag ttaaaacccc gctttttgaa gaaagggtgg
      961 aaaatagagg gcaaggagcg tttgggaggg acagtggagg aggttagaga ggttttatct
     1021 cgcctgggtt accgtccagt agaaatcgaa ggagtgattg atgacttatg gaatgaactt
     1081 gttaaagaag gagcagatgt ggagacctgg gtcagggagg ctttagtcaa gttaggtggt
     1141 ggggtttgaa aagagaatca gtggacgatt ttctcatacg gccgcaaagt ttagctgatt
     1201 ttgtcggtca ggaaaaagtt aagtccaatc tccaggttta cattcaagcc gctcgctcaa
     1261 gaggtgaacc cttagaccat gtgctattgt ttggtcctcc tggtctgggt aaaaccactc
     1321 tggctaatat catcgcccac gagatgaaca ccagccttcg ctatttatct ggccccactt
     1381 ttacaagagg cggagactta gcttctgttt ttagtacgct ttctgcgggg gaagtagtgt
     1441 ttatcgatga aattcaccgc cttcctccac cttgcgagga gattctctac ggtgctatgg
     1501 aagactttgc tcttcatctt gtggtgggaa aaggcccggg agcgagaagt atacgcattc
     1561 aattgcctct ttttactctg gtgggagcta ctacccgggt gagtcttctc agctctcctt
     1621 tgcgcaatcg cttcggtatt gtggagaagc tggattttta ccaagaagag gaaatttcta
     1681 aaatagtgaa gaggtcggca caggttatgg gagtggagat cgaacccggg ggagcagaag
     1741 aaattgctca tcgttcccgt ggcactcccc ggattgccaa tcgtctgctt cggagagtaa
     1801 gagattttgc tcagtttttt ggcagggaaa taatagatga agaaatagca aaagaggctt
     1861 ttaatcactt aggggtggat gaggtaggcc ttaccgactt tgatcgaaaa tttttagagg
     1921 tgctggttaa acattatcaa ggaggaccgg tggggattga aaatattgcc agcgctttag
     1981 gagaagatcc tgctactctg gaggaggttt gtgagccttt tcttctaaaa ttaggtttta
     2041 ttgcccggac cagaagaggg agaataataa ctgctcgagg tcgccaatat ctccagaagg
     2101 tgaaagaaat tgggtgataa aattctgctg catacctgct gtgctccctg tgccacctac
     2161 gttaacgaag ctttagaaaa agaaggatgg gaagtggtaa atatttttta taaccccaat
     2221 atccatcctt ttgatgaata tgctcgtcgt tatgaaaccc tggttttgta cctggagagg
     2281 gaggggaagg ttcttaaagc cccctttcct tacaaccctc tggaatattt ttctctcatt
     2341 actgaggcag aaaatcgctg tcctaagtgc tattttctgc ggttgcgaaa agtggctcag
     2401 tggggaaaag aaaggggttt tgaatttttc actaccacct taactatcag tccttatcaa
     2461 gatttatcag ccatctggga agtagggaaa caaattgagg aagaattggg tattaaattt
     2521 ctggtccgag attttcggga gggttttcgc cagagcgtga cccggtcgag ggagttgaag
     2581 ctttatcggc agaattattg cggttgtatt ttcagtaaat atgaaggagt aaaaaggagg
     2641 ctatggaaac gtcttactgg gctaaaattt tcttaattct gggtttggta ttcatagtgg
     2701 tgggagttct attgctcatt gtaccccgga ttccggggat aaataaactg gggcgcctgc
     2761 ctggcgatat agtttatcgg cggggtaatt ttgtctttta ttttcccctt acaacctgca
     2821 tcatcatcag cgtggttctc agccttcttt ttgctctctt cttcaggcgt taggaggaaa
     2881 aaatgcgaaa aagagttatc cttctcatat ttttggttct ttttgtctgt atgaatgggg
     2941 cctggggatg gggaaaagaa ctggaagtga aagttaattt gaataaagca aaagaagcgg
     3001 tatttagctc ctccggaggc ttaaaaatag aaataggaaa gcgttatttt tctgttcctt
     3061 cttcttatcc tgttcacttc agggaaaagg gagggaaggt agaatgggaa gaagaaaaca
     3121 tcctttcctc tgaagtttat atatatccta aaggcaaaga accaattttg tggggtaaaa
     3181 gaccctatcg gggaagtttt attgtgaaag catcaagcga tggtatttta gtgataaatc
     3241 agctccctgt tgaagattat atcaaaggca ctatgaagct ggaattgagt cctgcctggc
     3301 ctgtggaagc tctcaaagct caaattatag tttctcgcac ctatgctttg aaaaatttgc
     3361 gacgtcacga ggaagagggt ttcaatttct gtgctaccga ccattgtcag cgctacggag
     3421 gaattaatgc tgaagatccg gttaccaatc gcctggtgga ag
//
LOCUS       DAOPSS010000148         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_22020_length_3424_cov_11.059958, whole genome shotgun
            sequence.
ACCESSION   DAOPSS010000148 DAOPSS010000000
VERSION     DAOPSS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22020_length_3424_cov_11.059958"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..829)
                     /locus_tag="PKN31_07495"
     CDS             complement(287..829)
                     /locus_tag="PKN31_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOA99801.1"
                     /translation="MDFSREERLALLVISLVVVSGLGFIAWRNFSTPGVSEETLLVIQ
                     VDGAVNSPGIYRVKSGTRIFEALELAGGTKKDAQTEDMNLALPVYDGQRISIPSSTTT
                     SLKDRAATPEVRSALQMVNVNTASQKELESLPGIGEVIAQRIIEYREQNGSFSRSEEL
                     LKVKGIGEKKLEAIKDLITF"
     gene            complement(831..3290)
                     /gene="leuS"
                     /locus_tag="PKN31_07500"
     CDS             complement(831..3290)
                     /gene="leuS"
                     /locus_tag="PKN31_07500"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOA99802.1"
                     /translation="MLQDYDFTEIELKWQEIWQKKRIFEVEIDENREKYYVLEMFPYP
                     SGEPHVGHAKNYIIGDVVARYFMRQGYNVLHPMGFDAFGLPAENAAIQHGIHPAIWTQ
                     DKINQMREILKRLGISYDWRREVITCEPDYYRFTQWFFLLFYKNGLAYKKEAPVNWCP
                     SCSTVLANEQVIDGTCERCGTEVVRKKLDQWYFRITRYAEDLLKDMDLLGNWPEKVLT
                     MQKNWIGRSEGAYIDFLLDNGEKVTVFTTRPDTIYGATFFVLAPEHPLVDELVTGTPY
                     EEKVKEFREKMARKSEVERLSDVTEKEGMYIGQEILNPVSGEKIPIWIADYVLLEYGT
                     GAVMAVPAHDERDHQFAQKYGLPIRPVIKKIDGDEYCFTEPGIMVNSGPFSGLPSEEG
                     KKKVIEFLEERGIGKGAVSYRLRDWLISRQRYWGAPIPIIYCERCGEVPVPEEDLPVL
                     LPPDVDFLPGGPSPLARSPEFVNTTCPQCGGKARRETDTMDTFVCSSWYYLRYTSPEA
                     KDRPFRKEDVDYWMPVDQYIGGVEHAILHLLYSRFFTKVLNDLGLVDFREPFSNLFAQ
                     GMITKGGVKMSKSKGNTVSPREIINRFGADTVRVFILFAGPPELDMEWSDQGVEGAFR
                     FLNRVWRTVGRVIEFPTQEIEIDEGRMEALERKVHQTIFRVTRDIQEKFHFNTAISSL
                     MELLNEMIDYVRFLEEKGASSEEKEILVFASRTLVSLLNPIAPHLSEELWQLLGEKDL
                     LSTESWPVYQKEKMYEEEVEVIVQINGRVRSKVVVSRGSSKEEVLALALQDEKVKSWL
                     NHGEIKKSIYVPDKLINLVVG"
     gene            complement(3341..3416)
                     /locus_tag="PKN31_07505"
     tRNA            complement(3341..3416)
                     /locus_tag="PKN31_07505"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3381..3383),aa:Ala,seq:ggc)
BASE COUNT          964 a          842 c          595 g         1023 t
ORIGIN      
        1 caatctcgga gtgattttgt atgctgttac tgcaggatgt tatctttttt tgtatggtgt
       61 ggtaggtaat ttcatatttt ctcttgtgag aatccaaaat tgaaaccaac taccacaaca
      121 ctggagtgcc ttttctttag cgtaaaaatt tctttaattt ttttctcatc tttttagtct
      181 tctttaatct tacaccgtag acaattctat atttttgttt tttctaggag gtcacaaggg
      241 gacgccatct gttgttcggc tctgctatct atgcagaaaa cagcaattaa aaggtaatca
      301 aatcttttat tgcctccagc ttcttttccc ctataccttt tactttaaga agttcctcag
      361 aacgagaaaa agagccattc tgttctcggt attctatgat gcgctgggca atcacttcac
      421 caatccccgg taaagattcc agctcttttt gagaagcagt attaacgttt accatttgaa
      481 gagctgaccg cacctcagga gtggcagctc tatcttttag agaagtagta gtagaagagg
      541 gaatagatat tctctgtcca tcataaacgg gaagagctaa attcatgtct tcagtctgag
      601 catccttttt agttccccct gccagttcca gagcttcaaa aatacgagtt ccggatttga
      661 ctctataaat acccggagag tttactgctc catccacttg aataacaagt aatgtttctt
      721 cactgacccc gggtgtagaa aaattcctcc aagctataaa tcccagccct gaaactacca
      781 ccaaagaaat tactaaaagt gctaatcttt cctcacgaga aaaatccacc tcatcccacc
      841 accagattga ttaatttgtc tggtacataa atgctttttt taatttcccc atgatttagc
      901 cagcttttta ctttttcatc ctgcaaagcc agagctaaaa cctcttcttt agaagaacct
      961 ctggagacca ccaccttact cctcactctg ccgttgattt gtactatcac ttctacttct
     1021 tcttcataca ttttttcttt ttgataaaca ggccagctct ctgtggaaag caagtctttt
     1081 tcccctagaa gctgccagag ctcttcactc aggtgaggag caataggatt tagaagagaa
     1141 accaaggttc gagaagcgaa aaccagtatt tctttttctt cagagctagc tcctttctct
     1201 tctaaaaatc gcacataatc aatcatctcg ttcaaaagct ccatcaaaga gctgatagca
     1261 gtgttaaagt ggaacttttc ctggatgtct cgagttaccc ggaaaatagt ttgatgtact
     1321 ttacgttcca gagcttccat tcttccttca tctatctcaa tttcctgcgt tggaaattca
     1381 ataactctac ctactgttct ccacacccga tttaaaaatc gaaaggctcc ttccactcct
     1441 tgatctgacc actccatatc cagctccgga ggtccggcaa agagaataaa cacccttacc
     1501 gtatcagcac caaaacggtt aatgatttct cgaggactta ccgtgtttcc tttagactta
     1561 gacattttaa ctccaccctt ggtaatcata ccctgggcaa aaaggttaga aaatggctcc
     1621 cgaaaatcaa ctaaacctaa atcattaagc actttagtaa aaaaacgaga gtaaagaaga
     1681 tgaagaatag catgctctac ccctcctata tactggtcta ctggcatcca gtaatctaca
     1741 tcttccttgc ggaatggtct atcttttgct tccggagagg tgtagcgcaa ataataccag
     1801 gaagaacaaa cgaaagtatc catagtatca gtctctcgcc tggccttccc tccgcactga
     1861 ggacaggtag tattaacaaa ctctggactt cgagccagag gggaagggcc accaggcaga
     1921 aaatctacat ccgggggcag caatactggc aaatcttcct ccggcactgg cacctctcca
     1981 cagcgctcac aatatattat gggaataggt gctccccagt agcgctgtcg agaaattaac
     2041 caatctcgca gacgataaga cactgcacct ttacctattc ctctttcctc taaaaactct
     2101 attactttct ttttaccctc ttcagatggt agaccagaaa aagggccaga atttaccatg
     2161 atacctggct cagtaaaaca gtattcgtct ccatctatct ttttgatcac cggtctaatt
     2221 ggaagaccat atttttgagc aaattgatga tcccgttcat catgtgctgg aacagccatt
     2281 actgctcccg ttccatattc taagagcaca taatcagcta tccaaatggg aattttttct
     2341 ccacttactg gatttaaaat ttcctggccg atatacattc cttctttttc ggtaacatcg
     2401 gagagacgct ctacctcact tttccgagcc attttttccc gaaactcttt cactttttcc
     2461 tcatagggag taccggttac cagctcatct accagaggat gttcaggagc cagaacaaaa
     2521 aaagtcgcac cgtagatagt gtcgggacgg gtggtaaaaa cggtaacttt ttcaccatta
     2581 tccaggagaa aatcaatata tgccccctcg cttctaccta tccagttttt ttgcatagtt
     2641 aaaacttttt ctggccagtt tcctaaaaga tccatgtctt ttagtaaatc ttcggcataa
     2701 cgagtaattc gaaaatacca ttgatcaagt tttttgcgaa ccacctctgt tccacagcgt
     2761 tcacacgttc catctattac ttgctcatta gctaaaaccg tagaacaaga agggcaccag
     2821 ttaactggag cttctttttt ataagccaga ccatttttgt agaaaagaag gaaaaaccat
     2881 tgagtgaaac ggtaataatc gggctcacaa gttataacct ctcttctcca gtcatagctg
     2941 atacccaatc gcttcaatat ctccctcatc tggttaattt tatcttgggt ccagatagca
     3001 ggatgaatac catgctggat agcggcgttt tccgccggta aaccaaaagc atcaaagccc
     3061 atagggtgca aaacgttata accttgacgc atgaaatagc gggctaccac atctccgatg
     3121 atataattct tagcatggcc tacatgcggc tcgccagagg gatagggaaa catttctaaa
     3181 acatagtact tttccctatt ttcatctatt tctacttcaa aaatcctttt tttctgccag
     3241 atttcctgcc acttaagctc tatctctgta aaatcataat cctggagcac ctttaaacct
     3301 cccacaaaaa aagctcgccc ccgggggcga gcttatcatc tggtggagct gacgggagtt
     3361 gaacccgtga ccttctccac gccaaggaga cgcgctccca actgcgctac agccccttac
     3421 cttt
//
LOCUS       DAOPSS010000149         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_22289_length_3393_cov_7.985021, whole genome shotgun sequence.
ACCESSION   DAOPSS010000149 DAOPSS010000000
VERSION     DAOPSS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22289_length_3393_cov_7.985021"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKN31_07510"
     CDS             <1..360
                     /locus_tag="PKN31_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99803.1"
                     /translation="RRSPKPQAEGSNPAGRTKGAFRDILTVKKLLFTFLFLPGIVLSK
                     EEDPVQLFQCILQLNQSYPVHRSTCDIDIQAKIKIPLLLPFTINLNRKLNLEDNYREI
                     EITHILFSALGGRSFYY"
     gene            338..703
                     /locus_tag="PKN31_07515"
     CDS             338..703
                     /locus_tag="PKN31_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99804.1"
                     /translation="MVEAFITKGPLSFLSQEKWEDILSPFALAAEKVDSDRHYFWAVR
                     KEIVAHYYHKNLEELEMDNLGRVKKIFLANPTSRGEAEQEYARFKGYRVVKRTRGRAK
                     YNSVNIDFQAETRNYQLYP"
     gene            complement(685..2109)
                     /locus_tag="PKN31_07520"
     CDS             complement(685..2109)
                     /locus_tag="PKN31_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA99805.1"
                     /translation="MLKQLKNAPIRKLIVTIILFLFTGTAIVQGEELLSYSSIIQELR
                     SLWKEGREDILYLLVEDAPLFLASHELTGGIKKIVENRGYPFYLRKSALSLLSREMEG
                     KERGEYLISLFWKEPDIALRVEIIRLLGETDDWIPFFFSILRKEDSGNKEEEIILRWE
                     ALRALAHWKVRESLPFFFRLIEKEDFPSELKEEVDELLSLYDFSVSFYLFTMINSENP
                     FFREIALFALWEREDGDVVNYAHKMSNDRDKKVRNLSQAILWGEGEISHLTLQEEDLN
                     SRLIRVLAKYEKGVNLLIQMGEKDPLLEEQIFESLGKTEDIAFTPCLKEKLKEREWRP
                     FSLLRFLPLEENIEIVFSLLVSRGEELYLQEEEIPPLGKEDLRSNLYLIREILRSGDE
                     GAKLFILKRLPVLGEEALPLIEIAMEEASPLLQREIIRALEEIKAPAAAYFLEKLAFS
                     LDEEIKISARLALAKKYLSQGYSW"
     gene            complement(2162..3187)
                     /locus_tag="PKN31_07525"
     CDS             complement(2162..3187)
                     /locus_tag="PKN31_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOA99806.1"
                     /translation="MATIYDIAKLAGVSPATVSRALNQKGQVKEETRKKILEIAEELN
                     YSPNYLARSLKKKRTFTIALIISDVTNPFFTTLARGVEDKASDQGFNTIFCNTDEDPA
                     KEKEYIDLMLKKRVDGLVVASCSNGDTLLGLKEKEVPVVLVDRKIEDFDGDSVVGDSK
                     EAAYELIRHLTEVHGFREIAMITGPVTISTSKDRVAGYQRALQEAGIPVKPEWIMMGS
                     YKEDFGYQIATRFFSEKKKLPQAVFAGNNFIAIGIVRAARERGIKIPEDLALVVFDDL
                     ESASLLYPFLTVAKQPAYTMGSLATQFLLERIEKRGIREKREVVLKVETIIRRSCGCK
                     GSENNDF"
BASE COUNT          973 a          741 c          615 g         1064 t
ORIGIN      
        1 cggcggtctc ctaaaccgca ggctgagggt tcgaatcccg ccgggcgcac caaaggtgca
       61 tttcgggaca ttttaacagt gaaaaagctc ttatttacct tcctcttttt acccggtatt
      121 gttctaagca aagaagaaga cccggtgcag ctctttcagt gtattttgca gctcaatcaa
      181 tcttatcctg ttcatcgctc aacctgtgat atagatattc aggcgaaaat caaaattccc
      241 cttcttctgc cttttactat taacttaaat agaaaattaa atctagagga taattaccga
      301 gaaatagaaa tcactcacat acttttttct gcactgggtg gtagaagctt ttattactaa
      361 aggacctctt tctttccttt ctcaggaaaa atgggaggat attttatctc ccttcgctct
      421 ggctgcagaa aaagtagata gtgatcgcca ttatttctgg gcagtaagaa aggaaatagt
      481 agcccattat tatcataaaa atttagaaga gttagaaatg gacaatttag gcagggttaa
      541 aaaaattttc ctggctaatc cgacatcccg gggagaagca gaacaggaat atgccagatt
      601 taaaggttac cgggtggtaa agagaacccg gggcagggca aaatataatt cagtcaacat
      661 tgattttcag gccgaaacca ggaattacca gctgtatcct tgagaaaggt atttttttgc
      721 cagagccaat cgagcgctaa tttttatttc ctcatcaagg gaaaaagcta atttctccag
      781 aaaataggca gctgcaggag cttttatttc ctccagagct ctaattattt ctcgctggag
      841 gaggggagaa gcttcttcca tggctatttc aataagaggc aaagcctcct ctcccaggac
      901 gggcaatctt tttaaaataa ataatttagc tccttcatca ccagaacgga gtatttcccg
      961 aattaagtat aggttgcttc tcaaatcttc tttaccgaga ggaggaattt cttcttcctg
     1021 gaggtaaagc tcctctcccc gggagaccaa aagggaaaaa acaatttcga tgttttcttc
     1081 cagaggaaga aaacgtaaaa gagaaaacgg tctccactcc ctttctttaa gcttttcttt
     1141 aagacaaggg gtaaatgcga tgtcctctgt tttgcccaga gattcaaaga tttgctcctc
     1201 aagtaaaggg tccttctccc ccatctgaat tagaagatta accccctttt catacttagc
     1261 cagaactctt atcaggcgag aattcaaatc ttcttcctgc aaagtaagat gggagatttc
     1321 cccttctccc cagagaatag cttgagaaag atttctaact tttttatccc ggtcgttaga
     1381 cattttgtga gcataattta caacatcccc atcttctctt tcccacagag caaaaagagc
     1441 tatttctcga aagaaaggat tttccgagtt tatcatagtg aataagtaaa aagacacaga
     1501 aaaatcgtag agagaaagca attcatccac ttcttctttt aattcggaag ggaaatcctc
     1561 tttttctatc aggcggaaaa agaaggggag ggattctctc actttccaat gagctaaagc
     1621 ccggagcgcc tcccatcgaa ggattatctc ttcttcttta ttacccgaat cctctttccg
     1681 tagaatagaa aagaagaaag gaatccagtc atctgtttct cctaaaaggc ggattatttc
     1741 tactcgtaga gctatatctg gttccttcca gaatagagaa attaagtatt ctcctcgctc
     1801 tttcccctcc atctcccggg aaagaagaga aagagctgat tttcgaagat aaaaaggata
     1861 tcctctgttc tctactattt tctttattcc acctgtgagc tcatgggaag ctaaaaaaag
     1921 aggggcatct tcaactaaaa gataaagaat atcttctcta ccctctttcc agagactgcg
     1981 taattcttga attatagagg agtaagaaag caactcttct ccttgtacta ttgctgttcc
     2041 cgtaaagagg aagagtatga ttgtcactat taattttcta ataggcgcgt tcttcagctg
     2101 tttgagcata gattttcgag gtttttagct tctctcagta ccggaggaag ctaaagcact
     2161 tttagaagtc attattttca ctccccttac atccacagga acgacggatg atagtttcta
     2221 cttttaagac tacttccctt ttttcgcgaa ttcccctttt ttcaatcctt tctaaaagaa
     2281 actgagtagc cagactcccc atggtgtagg cgggttgttt ggctacagtg agaaaagggt
     2341 aaagaagaga ggcagattct aaatcatcaa aaacaactaa agctaaatct tcgggaattt
     2401 taattcccct ttctcttgct gctctaacta tgccgatagc tataaaattg tttccggcaa
     2461 atactgcttg aggcaacttt ttcttttcac tgaagaaccg ggtagctatt tgatagccaa
     2521 aatcctcctt gtaagaaccc atcatgatcc attctggctt caccgggatt cctgcttcct
     2581 gcaaagccct ctggtatccg gctactctat ctttactggt agagatggta acgggaccgg
     2641 taatcatagc tatttctcga aaaccatgaa cttctgtcaa gtgccggatt agctcataag
     2701 ctgcttcttt actgtccccc actactgaat ctccatcaaa atcttctatt tttctatcta
     2761 ctaaaaccac aggaacttct ttttctttta aacctagcaa ggtatccccg ttactgcaac
     2821 tggctactac caatccgtct actctttttt tcagcattaa gtctatatat tctttctctt
     2881 tagctggatc ttcatcagtg ttacaaaaaa tagtattaaa tccctggtca gaagctttat
     2941 cttctactcc tcgagccaga gtggtaaaaa agggattggt cacatcagaa ataataagag
     3001 caatggtaaa ggttcgcttc ttttttaaac tccgcgccag gtagttggga gaatagttga
     3061 gctcttcagc tatctccaga atttttttcc tggtttcctc ctttacttga cctttttgat
     3121 ttagagctct ggacactgtg gctggtgaca ctccagccag cttagctata tcatatatag
     3181 tagccattca agcctcccga ttgaaactac taagagagat tccttctctc tcaatagttt
     3241 tttgcattat ctcttaattt ttaatcttat accacagaaa tgctaatagg tttttcccca
     3301 acataatcgc ttttaactat tttactttat atattttttc ttttttgttc acgctatagg
     3361 ctctatggga tactttccct tctctttcaa aag
//
LOCUS       DAOPSS010000150         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_22783_length_3337_cov_8.304692, whole genome shotgun sequence.
ACCESSION   DAOPSS010000150 DAOPSS010000000
VERSION     DAOPSS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22783_length_3337_cov_8.304692"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..670
                     /locus_tag="PKN31_07530"
     CDS             98..670
                     /locus_tag="PKN31_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99807.1"
                     /translation="MRRWTVMGIVSFFLLLAISPLGAREKEEAILEGRAFVPFITSES
                     QVKERFFSRASITVIDLHTGRVVAEEETGMDGSYEIPVPPRRSYLVQFQKDNQIILDI
                     CERVKKGERYNLSFANARSTAMSLVLLHLFSGKKDPNIIITYKANRILQAESFPLLQQ
                     KVSEAIARGRNPEDDSEIRLLIDHIALSVQ"
     gene            complement(769..2277)
                     /locus_tag="PKN31_07535"
     CDS             complement(769..2277)
                     /locus_tag="PKN31_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOA99808.1"
                     /translation="MILSLRGKLLLGFGLLIVLLLVAVGLGYYSINEVNNAFLILSEL
                     QDYEEASLEEISAITARIDRIQGTLLLVGLLALAAGLISAFFLYRSILGPILELTRGC
                     EMVEKGDLRQEIEVKSKDEIGKVARSFNSMIQKLRELISQVLRVSSALASSSEELSSS
                     IEEISQATEEIAKTISQVAQGSTEQSTELEAISQETQKISQITHHLSEATQRNLNLIA
                     EMKKGLEKNRLSLYEIEKAMKVTSTEGENSEKEARKGQELLFLLAQNIQEISRVTQEV
                     AQSISTLEVRSQEISKIVDLITGIAEETNLLALNAAIEAARAGEAGRGFAVVASEVRK
                     LAEQSAQAAQQISALIEEVKNDTQMAVERMERAEDRVQEGVKESEEVAKNFQNILATV
                     QKVIQSVSNLGLVFEEAKNSQKITEKSAEEVAALSEDNAQMIQEIDSRIQGVSKSISS
                     VALVSEENAASSQEVSASTEEQSASLEELTSASRFLAQLAQELRDLAATFQV"
     gene            complement(2274..2768)
                     /locus_tag="PKN31_07540"
     CDS             complement(2274..2768)
                     /locus_tag="PKN31_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOA99809.1"
                     /translation="MPKEFQLVAFQLGEETYGVDISQVEEIIRLQPITKVPGAPDFVE
                     GVINLRGRVIPVIDLRKRFHLPPREETKNTRIVVVEVPPNTVGMIVDAVDEVLRISEE
                     KIEAPSLIISSIDTEYIKGVGKLENKLLILLDLAKVLTKEEKGALQTMKEEMEKREEG
                     EVEN"
     gene            complement(2747..2980)
                     /locus_tag="PKN31_07545"
     CDS             complement(2747..2980)
                     /locus_tag="PKN31_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99810.1"
                     /translation="MLYEEESLKELEEKVWGSFSEAIFLEDLARKLGAEIVDVWETVL
                     HMEKEGLISFYNWQYKTVIYKKGREEPCLKNSN"
BASE COUNT          889 a          784 c          607 g         1057 t
ORIGIN      
        1 tagttgctgt attttcgagt cggtggtagt atttcatttg taaattagct atcttgttta
       61 gatacttaga taccttgaga gggaggtatt agaaattatg cgtcgctgga ctgtgatggg
      121 gatagtgagc ttttttcttc ttttagctat ttcccccctt ggtgccagag aaaaagagga
      181 agctatcctt gagggtaggg cttttgttcc ttttatcacc tcagaaagcc aggtcaaaga
      241 gcgatttttt tcccgggcat caatcactgt tattgacctt cacaccggaa gagtggtcgc
      301 agaagaggaa acgggaatgg acggttctta tgaaatacct gttcctcccc gaaggtcgta
      361 tctcgttcaa tttcaaaaag ataaccagat aattcttgat atctgtgaac gagtgaaaaa
      421 aggagaaagg tataacctct cttttgccaa tgctcgctct acagcaatga gtcttgttct
      481 cctacatctt ttttccggga aaaaagaccc gaatattata attacctata aagctaatcg
      541 catccttcag gcagagtctt tccctcttct tcagcagaaa gtctccgagg ccattgcccg
      601 ggggaggaat ccagaagatg attcagaaat acggcttctc attgaccata tcgctttgag
      661 tgtccagtaa atgatgctct atataatttc tttcccggtc tcctgctttc ccagagtagt
      721 aataatgagg tctaaaaccc cgggtacaga gctcttcccc caaggtattc atacctggaa
      781 agtagctgcc aggtctctca attcttgagc caactgagcc agaaatcggg aagcagaggt
      841 taactcttct aaggaagcac tttgttcttc agtagatgca gaaacctcct gtgaagaagc
      901 agcattttct tctgatacaa gagccactga gcttatactc ttgcttactc cttgaatacg
      961 gctatcaatt tcctgaatca tctgagcatt atcttcagaa agagcagcta cttcttcagc
     1021 gcttttttcc gtgattttct gagaattctt cgcttcttca aataccaggc ctaaattact
     1081 aacactctgt atcactttct gaacggtggc aagaatattc tggaaattct ttgccacttc
     1141 ttctgattct ttcactcctt cctggacccg gtcttcagct ctttccattc tctctaccgc
     1201 catctgagtg tcgttcttta cttcttcgat taaagcagaa atctgctgtg ctgcttgagc
     1261 tgattgttca gccaattttc ggacttcact agccaccacg gcaaaccctc ttcctgcctc
     1321 tcctgctcgg gcggcttcaa ttgcagcatt taaagctaag agattggttt cctcggcaat
     1381 accggtaatt aaatccacga ttttacttat ttcttgggat ctcacttcca gcgtggagat
     1441 agattgagct acttcctgcg ttacccggga gatttcctgg atattctggg ctaaaagaaa
     1501 aagaagctcc tgccctttcc tagcttcttt ttcactattt tctccttcgg tgctcgttac
     1561 cttcatggct ttttcaattt cataaagaga gagccggttc ttttccagtc cttttttcat
     1621 ctctgctatt aaatttaggt tcctctgagt ggcttcactc aggtggtggg taatttgaga
     1681 aatcttctgg gtctcctggc taatggcttc taactcggta ctctgctcgg tagagccctg
     1741 tgccacctgg gaaatggtct tggcaatctc ttctgtagct tgagaaatct cctcaatcga
     1801 ggaagagagt tcttcacttg aggaagccag agcagaagaa actctcaaca cctgacttat
     1861 gagctcccgt agcttttgaa tcatagagtt aaaactacga gctactttcc ctatctcgtc
     1921 tttactttta acttctattt cctgacgaag gtctcctttt tccaccatct cacaacccct
     1981 ggttaattca agtatgggtc ctaaaatact gcgatagaga aaaaaggcag aaatcaatcc
     2041 tgcagctaaa gctaaaagac ccactaaaag cagggtgccc tggatccggt ctatacgggc
     2101 agtgatggcg ctgatttctt ctagggaagc ctcctcgtag tcttgcaatt cagaaagaat
     2161 aagaaaagcg ttatttacct cattaatgga gtaataaccc aatcctactg ccactaataa
     2221 aagaacaatc aataagccaa accctaagag cagttttcct cgcagggata aaatcaattt
     2281 tccacctctc cctcttctct tttctccatt tcctctttca tagtttgcag agcacctttt
     2341 tcttctttag ttaacacttt ggctaaatct aaaaggataa gaagcttgtt ttctaatttc
     2401 cctactcctt ttatatattc agtatcgata gaagagatta ttagagaagg agcttctatt
     2461 ttttcttcac taattctcaa aacctcatcc acggcatcta caatcattcc caccgtatta
     2521 ggaggaactt ctactaccac aattctcgta tttttagttt cttcccgagg aggaaggtga
     2581 aatctcttcc ggaggtcaat caccgggatt actcttcctc gtaaattaat tactccttct
     2641 acaaagtctg gagctccggg aaccttggta ataggttgca aacgaatgat ttcttccacc
     2701 tgagatatat ccaccccata agtttcttct cccaactgaa aagccactaa ttggaattct
     2761 ttaggcaagg ttcttccctc ccttttttat agattacggt cttatattgc caattataaa
     2821 aggaaatcaa tccttccttt tccatatgaa ggacggtttc ccagacatcc actatttccg
     2881 ctcctaactt acgggctaaa tcttctaaga atattgcttc agaaaaggaa ccccaaacct
     2941 tctcttctaa ttccttcagc gattcttcct cgtacagcaa gactaaccct ttctcgatga
     3001 atctttttct tccttacttc ccgaagagga gctttttttt gaaagaggaa caatgattaa
     3061 attccgatag ttgaagaatg ccgtctggtt atttcttacc tgtaccattt tcatatctac
     3121 tcttacctcc tcttataaat attatcggca gcttctccca aaactttaga gccaggaatt
     3181 tttttgaaaa aagaaatctg tgtattcagc gcaatggaaa tccagcacac aacagaactc
     3241 cattgccagc acccttaaaa taaactacct ttttagatgc tgaatctaaa gggggtaaag
     3301 aaaggatgct aacaatggag caaatatatc atatcag
//
LOCUS       DAOPSS010000151         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_22831_length_3331_cov_9.559524, whole genome shotgun sequence.
ACCESSION   DAOPSS010000151 DAOPSS010000000
VERSION     DAOPSS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22831_length_3331_cov_9.559524"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="whiA"
                     /locus_tag="PKN31_07550"
     CDS             complement(<1..885)
                     /gene="whiA"
                     /locus_tag="PKN31_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOA99811.1"
                     /translation="MDFRTFLKDEIYAQIPSSLPMCRAEWWGIFRGGGTIKRRESNSY
                     LSFSHTDLSLIKKCLWLKNKLYPHLPHFLLQKEKRGINAGKWYSLDILWEENFWEEGG
                     FSDASLLKDMLEGKTARAFLRGLFEARGYISDPLHHYHLEIGPLPSDLVFVVLEHLEE
                     KNLPFQFRERKKENYIYTKNSSVIGSFLHYLGASQAYLQWEKLAIEKSTINDLTRWVN
                     WETANLERTVNSSLRQRQKLQEINLDSLPPRLQEVARLRLEHPYASLREIGDLCSPPL
                     SKSGVYHRLREIEKIAEEQ"
     gene            complement(875..2689)
                     /gene="uvrC"
                     /locus_tag="PKN31_07555"
     CDS             complement(875..2689)
                     /gene="uvrC"
                     /locus_tag="PKN31_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOA99812.1"
                     /translation="MTRRSYTRFEVKKEDWNKLPPECGVYTFRDSRGEIIYVGKAKNI
                     FKRVNSHLHHRYSSPLKRDMAEEIDTIGYFLVPDEHQALLLENELIKTHQPRYNLRLK
                     DDKSYPYIVFSPDGDFPAVRVARRKTTPFGQFFGPFTDVKKVKEGLKLLRSLFLLRGC
                     AISEKRFPLDRPCLDFELGLCCAPCLGSVTKEEYGERVKKAQDFLNGDYERVLSWLEQ
                     EMWKRAEELDFEEAARYRDRWEAVNKIVARYQLVLSRAEDVDFIDMAGDETLASLIVL
                     RVRKGRLIGSESFLTSGGKREEILREFGEEFYLNRFNLPSRVCWEGGEIPEIEEEYAI
                     TFTPPLTREEKEMMTFARSNAEKNLEVEKLKMEKRERASEKAKEELQALLGIPSPPEW
                     VEGVDISTFQGGETVGAVVSFKNGLPDKNNYRKFIIRETDYPNDYECLREVLERHLRS
                     LQEKSSPFPDLILVDGGIGQLNVALEVLENLGVEVPVVSLAEEWEDIYLPGEHQPLRL
                     PPHSEALQFLQRVRNEAHRFAITFHRLRRNKQSFGSILEEVKGIGPKRKKKLLSAFND
                     LREILEADSREVSKNLGIPEKTIEEVKKQLEKNLSRGF"
     gene            complement(2655..>3331)
                     /locus_tag="PKN31_07560"
     CDS             complement(2655..>3331)
                     /locus_tag="PKN31_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA99813.1"
                     /translation="GRFSFNVKGGRCEACQGNGTIKIEMHFLPDVFVTCEHCKGKRYA
                     RETLDIKYKGKNIAEVLDLTVSEALSFFENIPTIRRKLEILDRVGLGYIRLGQPATTL
                     SGGEAQRIKLARELSKIATGRTLYLLDEPTTGLHFADIEKLLDVLLELRSRGNTVLII
                     EHNLDVVKSCDYLIDLGPEGGEKGGYLIAKGTPEEVAKNPASYTGQFLRRVLSLDQEE
                     LYSVRS"
BASE COUNT          913 a          824 c          618 g          976 t
ORIGIN      
        1 ctgctcttct gctatttttt caatctctcg caggcgatga taaactccgg atttagaaag
       61 aggaggagaa caaaggtctc ctatttccct aagagaagca taaggatgtt ctaatcgcag
      121 gcgggcgact tcctgaaggc gaggaggtaa agagtccagg ttaatttcct gaagcttctg
      181 gcgctgacgc aaggaagagt ttaccgttct ttccagatta gcggtttccc agtttaccca
      241 ccgggtgagg tcgttgatag tggatttttc tatggccaat ttttcccatt gcaggtaagc
      301 ttgagaagct cccagatagt gcaagaaaga accaataacg gaagaatttt tggtataaat
      361 ataattctct ttttttcgct cccgaaactg aaagggtaaa ttcttttcct ccagatgctc
      421 aagaactaca aaaaccagat cagaaggaag agggccaatt tctaagtgat aatgatgcaa
      481 ggggtccgag atataacccc gggcttcaaa caaccctcgc aaaaaagcac gggcagtttt
      541 cccctcaagc atatctttca agagagaagc atcagaaaat cctccctcct cccagaaatt
      601 ctcctcccag aggatatcca gagaatacca ttttccggca ttaatccctc ttttttcttt
      661 ttgcaagaga aaatgaggaa ggtgaggata aagtttattt ttcagccaca aacacttttt
      721 tattaaactt aaatcggtat gagaaaagga aagatagcta ttactttctc tccttttaat
      781 tgttcctcct ccccgaaata ttccccacca ttccgctcga cacatgggaa gagaggaagg
      841 aatctgagcg taaatttcat ctttgagaaa agttctaaaa tccacgagat aagtttttct
      901 ccagttgttt tttaacttct tctatagttt tttcgggaat acccaggttt ttactaactt
      961 cccgagagtc ggcctctaaa atctctctta aatcattaaa ggcagaaaga agttttttct
     1021 tcctctttgg tcctattcct ttcacttctt ctaaaatcga accaaaagat tgtttattac
     1081 gccgcaagcg gtgaaaagtg atggcaaagc ggtgagcctc gtttctcact ctctgcaaaa
     1141 attgtaaagc ctcagagtga ggaggcagac gcaagggctg gtgttccccg ggaagatata
     1201 tatcttccca ctcctcagct aaagatacaa caggaacttc aactcccagg ttctccaaaa
     1261 cctccaaagc cacattaagt tgccctatcc ccccatctac aagaattaaa tcgggaaaag
     1321 gagaagattt ctcttgaaga gaacggagat gtctttctaa tacttctctc aaacattcat
     1381 agtcattagg atagtcagtt tctcgaatga tgaacttccg ataattattt ttatccggta
     1441 atccattttt gaaggaaact acggctccca ctgtttctcc tccctggaaa gtggagatat
     1501 ccactccctc tacccactcc gggggtgacg gaatacctaa gagggcttgt agctcttctt
     1561 ttgctttttc tgaagctctt tctctttttt ccatttttag tttttctact tctaaattct
     1621 tctccgcatt actccgagca aaagtcatca tctctttttc ttcccgggtt agaggaggag
     1681 taaaagttat agcatactct tcttctattt caggtatttc tcctccctcc cagcacaccc
     1741 gggaaggaag gttaaaccta ttcaaataaa attcctcccc gaattctctc aatatctctt
     1801 ctctcttccc gcctgatgtg agaaaactct cgcttcctat caagcgtcct tttctcaccc
     1861 ggagcactat aagagaagcc agggtttcat cgccagccat atcaataaaa tctacatctt
     1921 ctgccctgga gagaaccagt tgatatcgag ccacaatttt gttcactgct tcccacctat
     1981 cccgataacg ggctgcctct tcaaaatcca gctcctctgc cctcttccac atctcctgct
     2041 ccagccagga aagaactctt tcataatcgc cattcaaaaa atcttgagct tttttcactc
     2101 tctctccgta ttcctcttta gttactgaac ccagacaggg agcacaacag agacccagtt
     2161 caaaatccag acagggtcta tccagaggga aacgcttctc cgaaatagcg cagcctcgca
     2221 agagaaaaag agagcgcaga agtttcaaac cttctttcac ctttttcaca tcggtaaatg
     2281 ggccaaaaaa ctgaccaaaa ggagtggttt ttcgccgggc tactctcact gctggaaaat
     2341 ccccatcggg agaaaaaact atataggggt agcttttgtc atcttttaag cgcaggttat
     2401 aacggggctg gtgagtttta attaattcat tctccagaag aagagcctgg tgctcatcag
     2461 gaaccagaaa ataaccaatg gtgtctattt cttctgccat atcccttttc aggggagaag
     2521 agtaacggtg atgaagatga gaattcaccc gcttgaaaat gtttttcgct ttacccacat
     2581 aaataatttc tccccgggaa tctcgaaaag tgtacacccc gcactccggg ggtaatttat
     2641 tccagtcttc ctttttaact tcgaaccgag tatagctcct cctggtcaag ggaaagcact
     2701 ctcctcaaaa attgcccggt ataggaagcg ggattttttg ccacttcctc aggggttcct
     2761 ttggctatta gataccctcc tttttctcct ccttctggtc ctaagtcaat cagataatca
     2821 caactcttga ccacgtctaa attgtgctca attattaaaa ctgtatttcc ccgactgcgc
     2881 aactccagca gcacatccag cagtttttct atatcggcaa aatgaagtcc cgtagttggc
     2941 tcatccagaa gatatagagt tctaccggtg gctattttac tcagttctcg agctaacttt
     3001 attctctgcg cctctcctcc tgagagcgtg gtagccggtt gccccagccg gatataacct
     3061 aaccccaccc ggtctaaaat ctctaatttt ctccggatgg tcggtatgtt ttcaaagaaa
     3121 gaaagagcct cgcttactgt caaatccaga acttcagcaa tgtttttacc tttatattta
     3181 atatctaaag tctcccgggc atatcttttg cctttacagt gctcacaggt aacaaacaca
     3241 tcggggagaa agtgcatttc tattttaatc gttccgttac cctgacaggc ttcacacctt
     3301 cctcctttaa cgttaaaact aaagcgtccc g
//
LOCUS       DAOPSS010000152         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_23058_length_3306_cov_4.853584, whole genome shotgun sequence.
ACCESSION   DAOPSS010000152 DAOPSS010000000
VERSION     DAOPSS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23058_length_3306_cov_4.853584"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..94
                     /locus_tag="PKN31_07565"
     CDS             <1..94
                     /locus_tag="PKN31_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOA99814.1"
                     /translation="FTRAQILLQAGTAMLAQANILPQSVLQLLG"
     gene            171..539
                     /locus_tag="PKN31_07570"
     CDS             171..539
                     /locus_tag="PKN31_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOA99815.1"
                     /translation="MEINPLSRNDAEQIISFLVKEEAKSEVNLASSFMSAENHEETEM
                     VEEIEKEDWVWFVRALQDIFRGFNLGITFRVHEETERIIARVIDRETKEVIREIPPEK
                     FLDMIARLQELAGVFIDEMV"
     gene            551..1930
                     /gene="fliD"
                     /locus_tag="PKN31_07575"
     CDS             551..1930
                     /gene="fliD"
                     /locus_tag="PKN31_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOA99816.1"
                     /translation="MAGNLSIDGLISGLDTTDLINQLMEVERIPITQLEARKAGYNQK
                     ISLWQAANTKLLALLTSAGSLTSASTFQAKKVEVSNSDILSATASSSAVSGSYQLRVE
                     RLAYAHQMATTSTYADYDTTTFGEGTITLQVGEGDEVEIAIDSSNNTLEGIKNAINQA
                     DAGVQASIVKVSEGYKLLLASETTGEAGEISITSALTGGGATLSSFDTIQPAQNARIV
                     LGSGSSALVYESSSNVITDFLPGVTLNLKAVSTEVVTLKVDKDLEGIKNSILDLVRRY
                     NDFSQFVKDNTFYDADTKEGGALLGDTTLSRIMSGINGKLFGSVSSPNEKYDSLVALG
                     ISIDRYGQISVDEEKLSKALEDDLGAVENVFRGNKGVAVALKGYLDFVTDPREGTLKD
                     TQDLYKNLIKDIDERIEVMEERLEKQAERLRTQFTAMESALSLLQTQATWLSQQITAL
                     SSFNYYQGK"
     gene            1988..2371
                     /gene="fliS"
                     /locus_tag="PKN31_07580"
     CDS             1988..2371
                     /gene="fliS"
                     /locus_tag="PKN31_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOA99817.1"
                     /translation="MGAYNAALIQKRYQENAVSTASPLRLVIMLYGGVLRFIRVAQKS
                     FSGGDEREGKNNLLQVEKIVLELIGSLNLEEGGEVARNLLRIYQFTLEQCALCNGNNY
                     SQILPPLLKVWEGLEESWREIEKNG"
     gene            2364..2720
                     /locus_tag="PKN31_07585"
     CDS             2364..2720
                     /locus_tag="PKN31_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99818.1"
                     /translation="MAETESDAIVLLKALQEITIKMKEKLGKGEIEEFYSFLSRRQEI
                     IEELDKIGSIQDKEALSLLLEINDLSQEMQKNLSQVMEKELQNIKNMQESLHALRHFR
                     SNLQPPSPPRFLDQKR"
BASE COUNT          986 a          643 c          836 g          841 t
ORIGIN      
        1 gttcaccagg gcacagattc tcttgcaggc gggcacggcc atgttagctc aggccaatat
       61 cttgccgcag agcgtgttgc aacttttggg ttaatataga cccatggggg aggggggaaa
      121 ccctctccct tttatttttt tagagaaagt aaaaggccga ggtgaagtct atggaaatta
      181 accctctatc gagaaatgat gcggagcaga taattagttt tctggtaaaa gaggaggcaa
      241 agagcgaggt gaatcttgct tcttctttta tgtcagcaga aaatcacgaa gaaaccgaga
      301 tggtggaaga gatagagaag gaagactggg tgtggtttgt gcgggcgcta caggatatat
      361 tccggggctt taacttaggg atcacttttc gagttcatga agaaaccgag cgaatcatcg
      421 cccgggtaat tgaccgggaa actaaggagg ttattcgaga aattcctccg gagaagtttt
      481 tagatatgat agcccgcttg caagagttgg caggagtttt tatagatgaa atggtgtgag
      541 gtgatataga atggcaggta atttaagtat cgatggtttg atttccgggc tcgacactac
      601 ggatctaata aatcagctta tggaggtaga gcgcatcccc atcactcagc tggaagcgag
      661 aaaggcaggc tataaccaga aaatttccct ctggcaggca gccaatacca aactcctggc
      721 tcttcttacc tcagcaggta gtttaacttc tgcttctacc tttcaggcga aaaaggtgga
      781 agtgagtaac tctgatattc tctccgccac cgcttcttct tcagcggtga gtggaagtta
      841 ccagctccgg gtggagcggc tggcttatgc tcaccagatg gccaccactt ctacttatgc
      901 tgactacgat accactactt ttggtgaggg gacgattaca cttcaggtgg gagaagggga
      961 tgaggtggag atagcaattg actcttccaa caacaccctg gaggggataa aaaatgctat
     1021 taaccaagcc gatgccgggg tgcaggcttc cattgtgaaa gtatctgaag gatataagct
     1081 ccttttagcg agtgagacaa ccggagaagc aggggagatt tctataactt ctgctctaac
     1141 aggaggaggc gccactcttt cttcttttga tactattcag ccggctcaaa atgctcggat
     1201 agtgctggga agtgggagtt ctgctttggt ttatgaatct tcttccaatg tgataactga
     1261 cttcctcccg ggagtgactc taaatctcaa agcagtaagc acagaagtcg tgaccctcaa
     1321 ggtggacaaa gatttagagg ggataaaaaa cagcatttta gatctggtgc gaaggtataa
     1381 tgattttagc cagtttgtaa aagataacac tttttatgat gcagacacca aagaaggggg
     1441 agcgttactc ggagatacta ccttatcccg gattatgagt gggataaatg gtaagctttt
     1501 tggtagcgtt tcttctccta atgagaaata tgatagcctg gtggcgctgg ggatttcaat
     1561 tgaccgctat ggacaaattt cagtagatga agaaaaactg agcaaggctc tggaggatga
     1621 cctgggagcg gtggaaaatg ttttccgagg taataagggg gttgccgttg ctcttaaggg
     1681 atatttagat tttgtcaccg atccccgaga agggaccttg aaagacactc aagacctcta
     1741 caaaaacctt atcaaagaca ttgacgagcg gatagaggtt atggaagagc ggctggaaaa
     1801 acaagcggag cgcttgagga cacaatttac tgctatggag agtgctctct ctcttttgca
     1861 gactcaggca acctggctca gtcagcagat tactgccctt tcgagcttta actactatca
     1921 ggggaaatga gccgggtggg aaataaaagt ttaaatagaa taaaaacaga aaaaggggga
     1981 gtggaaaatg ggagcttata atgctgcttt gattcaaaag cgttatcagg aaaatgcggt
     2041 gagcactgcc tctcccctga gactggtgat tatgctctat ggtggagtgc tccgttttat
     2101 acgagtagct caaaaatctt tttccggggg agatgaaaga gaagggaaaa acaatcttct
     2161 acaagtagaa aaaattgttt tggaactgat aggttccctg aacttagaag aagggggaga
     2221 ggttgctcgc aatctattga ggatttacca gtttactctg gagcaatgtg ctctctgcaa
     2281 cggaaataat tattctcaaa tccttccccc tcttttgaaa gtctgggaag ggctggagga
     2341 gagctggcga gagatagaaa aaaatggctg aaactgaaag tgacgcgata gttcttttaa
     2401 aggctttgca agagataacc attaagatga aagaaaagct gggaaaaggg gagatagaag
     2461 agttctattc ttttctttcc cgccggcagg agataataga agagttagat aagataggtt
     2521 ctatacaaga taaggaggct ttgtctctac tgcttgagat taacgacctc agccaggaga
     2581 tgcagaaaaa cctctcccag gtgatggaaa aggagcttca aaacatcaaa aatatgcagg
     2641 aaagcttgca cgctctccgc cattttcgct ccaacctcca gcctccttct ccccctcgct
     2701 ttttagacca aaagaggtaa agtagtctct ttaaaaaata aattgtgata agtggagtct
     2761 atagttttcc ccttctctta cagcgggata acctaacggt gcagaacaga actataagtg
     2821 agcgttactc cccccttatg actccgaact acaaaggggc gttgctcttt ataaacccct
     2881 gaacgcctct atcatccttt gctcatcacc ccggcaactt tcgagccggg gtctaaatta
     2941 tttaaggacg tggattccca atagaagatt ttgggaatga caaacgagag agttcctcga
     3001 ctcttttttt ctttccctct cgaagggaga gggtgagggt gaggatgagg atgaaataac
     3061 tataaaaggg gcgccgcccc ccttataaac cctttaaaaa aggaaaaaaa cataaagaca
     3121 aaaagaaaaa gcaaaaacat ggatgcctga taaaagattt caggcatgac gaaaaaagga
     3181 tgtcacccct gaatgtttct atcacctcaa gtccgtcacc ccggcaatct ttaagccggg
     3241 gtccataagt atttaaaaat atggattccc gataaaaaat ttcgggaatg acgaatgatg
     3301 tggatg
//
LOCUS       DAOPSS010000153         3284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_23271_length_3284_cov_7.004645, whole genome shotgun sequence.
ACCESSION   DAOPSS010000153 DAOPSS010000000
VERSION     DAOPSS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23271_length_3284_cov_7.004645"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1344)
                     /locus_tag="PKN31_07590"
     CDS             complement(151..1344)
                     /locus_tag="PKN31_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOA99819.1"
                     /translation="MLRRWHLILLITEIVLLSLFLSSCSQPIEGNLLGVDLGDLKKSE
                     LSSFLEIMEERWLESPLSLKVNGLTISLNKKELGISWNIEAMKRFILKGEGETVPLMF
                     SWDEEKVKSLLDDLAQKTYSTPEDARLEEKRVIPSREGKRLDEERAILELREALSRGK
                     EEITLTTFQLLQPKIDTSIILEKLGTPHLLARYQTSLEGKDEPTVYNIQKASSSINGY
                     ILEKGEIFSFNNIVGRAEKEDGYLETNIMVNGRLSPGYGGGVCQVSSTLYNALLGTEA
                     QILERHPHSGYSETTSYVPPGRDAAVSYGSKDLRFFYPTQKVVIFAYLQEESLICEIW
                     GEKENSLQKTWETQIVSLERKGEKEGLLTVETTVRNGEKIEYRYQDTYLIPWELEQKI
                     KEEFQ"
     gene            complement(1374..2447)
                     /locus_tag="PKN31_07595"
     CDS             complement(1374..2447)
                     /locus_tag="PKN31_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOA99820.1"
                     /translation="MLRSLRKHLDVILIIVVIAFAVTIYYGYGSRRSGGGNQAVAATV
                     NGTAITFYELDQAFRNLISQYDSKTLNQMDENSITFLRRLILENLIQNELLYQEAKSR
                     RIKVSSSEIEEQIELIKAQFPSEEDFRRYLGYQGFNLAALREAIKRELMIQKLEDSLA
                     EEITIPEEEIQKYYEENKSLFTTPPQYHLRQMTFSSQEEAEAVLKRLYVGEDFAQLAK
                     DNSQDSYASQGGDLGWVSETTLPPEVKEVLAPLKDKPLAITPVIKVGEHYCIYQIIEF
                     KPQEEKKYEEVKENIEKLLKEEQKRVKVNHLVAELREKSKITIAESLQIAVESSSPEA
                     DTAPQSSPEATGKEENREEIPTP"
BASE COUNT          900 a          676 c          664 g         1044 t
ORIGIN      
        1 gtctggcagc aggagctaaa cctgggagag gtatataaaa ataaacccac aatagacatc
       61 gaagtgattt cccgcgataa ataaagggaa gaataaaaaa caccggggtg ggatggctca
      121 tcccggtgtt tgtagaaatt taaaaatctg ttattggaat tcttctttaa ttttttgctc
      181 caattcccag ggtatcaagt aggtatcctg ataacgatac tcaatttttt ctccatttct
      241 tactgtagtt tctacggtta agagtccttc tttctctccc tttctctcta aggaaacaat
      301 ttgcgtttcc caggtttttt gcagggagtt ctccttttct ccccatattt cacatattaa
      361 gctctcttcc tggagatagg caaaaataac tactttctgt gtaggataga agaatcgaag
      421 gtctttactc ccatagctta ccgctgcgtc tcttcctggg ggaacataag aggtggtttc
      481 tgaatatccg gaatggggat gacgttctaa aatttgagct tcagtgccta atagagcatt
      541 atataaagta gaagatacct ggcatactcc tcctccataa ccgggagata gtcttccgtt
      601 aaccattata ttggtttcga ggtaaccatc ctctttttct gctctgccta ctatgttgtt
      661 gaaggagaaa atttctcctt tttctaatat gtaaccgttg atggaagaag aggccttttg
      721 aatattataa actgtgggtt cgtctttacc ttcaagggaa gtttgataac gggctaatag
      781 atgaggggtt cccagttttt caagaataat ggaggtgtca attttcggct gcaggagttg
      841 aaaggtagtg agagttattt cttcttttcc ccgggagaga gcttctcgga gctctaatat
      901 tgctctttcc tcatcaagcc ttttcccttc ccgggaggga attacccttt tttcctctaa
      961 gcgagcgtct tctggggtag aataagtttt ttgagctaag tcatccagga ggcttttgac
     1021 cttttcttca tcccaggaaa acataagggg aactgtttct ccctctcctt tgaggataaa
     1081 ccttttcatt gcctcaatgt tccaggaaat tcccagttct tttttattga gggaaatggt
     1141 taaaccattc actttaaggg agagaggaga ttccaaccac ctctcttcca ttatctctaa
     1201 aaaagaggat agctcgcttt ttttaaggtc gcctaaatca acacctaata gattgccttc
     1261 aattggctga gagcaagaag aaagaaacaa agataaaagt actatttctg taattaataa
     1321 gattaggtgc catctacgga gcatggttat tttctaatac ttttctttag aattcacggg
     1381 gtaggaattt cttctctatt ttcttcttta ccagtcgctt ccggagaact ctgaggagcg
     1441 gtgtcggcct ctggagatga gctttctacg gctatctgca aggactcagc tatggtaatt
     1501 ttacttttct cccgcaactc agccactaga tggttaactt tgactctttt ttgctcttct
     1561 tttaaaagct tttctatgtt ttcttttacc tcttcatatt ttttttcttc ctggggttta
     1621 aactctatta tctgatagat acagtagtgc tctcccacct taattacagg agtaatagcc
     1681 agaggtttgt ccttgagagg agcgagaact tcttttacct cgggaggcaa ggtagtttca
     1741 ctcacccatc ctaagtctcc tccctgagaa gcataggaat cttgagaatt atctttagcc
     1801 agctgggcaa aatcttctcc tacatacaaa cgttttaaga ctgcctcggc ctcttcttga
     1861 gaagaaaaag tcatctgccg caggtgatat tgaggaggag tggtgaaaag ggatttattt
     1921 tcttcatagt atttttgtat ttcttcttct ggtatggtga tttcttctgc caggctgtct
     1981 tccagttttt gaatcatcaa ttctcttttt atggcttccc gcagagcagc gagattgaat
     2041 ccctggtatc ctaaatatct tcgaaaatct tcttcagagg ggaattgggc cttgataagt
     2101 tctatttgtt cttctatttc agaagaggaa accttaattc ttctcgattt ggcttcctgg
     2161 tagagaagct cgttttgaat caggttttcc agtattaaac ggcgcaagaa agttatagaa
     2221 ttttcgtcca tctggttgag agttttagaa tcgtactgag aaattagatt acgaaaggct
     2281 tggtccagtt cataaaaagt gatagctgta ccattgactg tagctgctac tgcctggttg
     2341 cctccccctg agcggcgaga accgtaaccg taataaatag ttacagcaaa agctattacc
     2401 acaattatga gtattacatc taaatgcttg cgcaaacttc tcaacattca gtccacctct
     2461 gtgacttcta agtttttaac atctttagaa tactactcca ttagttcatt ttctgtcaac
     2521 tgcttagggg cttttgtgtg gttattctgt gataatgtca cccttaaaac tcttgaaaag
     2581 ctgcttcttt agtagcagaa tgaaagatta ataggacagc ttgcggtgtc cccctcctta
     2641 tcatcgtaaa aagcgaagtg ctccgcggcg atctctcttt tttgtcaccg cgaggcccgt
     2701 gacagcttct tgggtggatt atgtgtagga aaaacgaaaa agaatagtct gcggcgctaa
     2761 aaacaaaagg caaacaaaaa aacatgaaaa tgcaaaaacc actgggagag gaaaacagac
     2821 agcctgcagc gtaagattaa aagactaaaa aacctaaaaa gcaaagaccg ctggtgggaa
     2881 aaccatccac catataaagg gaaaagaatt cccctttatc aattcctttc ttttctttct
     2941 ctctctcttt tttccgggga gagggttaga gtgagggtga atcaactata aaagggcgcc
     3001 gccccctata agcgcccccc ggaaaagacg aaaatacaaa aagacaaaat aaaaagcaaa
     3061 aacatggatt cccgataaaa aatttcggga atgacggagg gggttgttcc tgaatgtatt
     3121 tattgccttt ttccgtcacc cctgaatgcc tctatcatcc ccaagtccct cattcctgag
     3181 tggttctatc atccccaagt ccgtcattcc tgagtggttc tatcaggaat ctaaattatt
     3241 taagggcaag aatatttgga tgcctgataa aagatttcag gcat
//
LOCUS       DAOPSS010000154         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_23386_length_3273_cov_9.181479, whole genome shotgun sequence.
ACCESSION   DAOPSS010000154 DAOPSS010000000
VERSION     DAOPSS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23386_length_3273_cov_9.181479"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PKN31_07600"
     CDS             complement(<1..1001)
                     /locus_tag="PKN31_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA99821.1"
                     /translation="MDKIGCGVIGAGTWGEAHARIFSSEPYSELIAVCDLVEEKARHL
                     AQKYGAKRWYTDIEKLLKDPDIQAVGITTPDFAHREPFVAACQAGKDILVEKPLATNK
                     EDLEAMKDAFQKSSSRVMVDFHTRWNPPIVIAKESIDKGELGELISMYYRLNDIVYVP
                     TQMLSWAEKSSILWFLGSHTVDTLRFLSNSEITRVYSVSRSKVLVQKGINVPDIYQSI
                     LEFENGVIATIENNWIIPNTNPHWNDIKINILGSKGMINMDLTNNQAIERYLEDKSDH
                     PDILIMPLIHGKASGFAHESIRDFVNRLYAKQEFIAGFEDGYRVSMVVFSLLSSAEK"
     gene            complement(1025..2200)
                     /locus_tag="PKN31_07605"
     CDS             complement(1025..2200)
                     /locus_tag="PKN31_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOA99822.1"
                     /translation="MVTLSSSKINDIHKKMIFRFIWEEGKVSRSQIGKAFGLSKSTVS
                     GIVRELIEEGLIVESGWGNSSGGRRSELLSLNPYGPLILSVFLKDGGEGEVAVINLLG
                     TVLDNRHFYLSSKEDAKETVTKISYFLRFFLSSYPENCFLGIGLGVPGIVDHKQGTIS
                     YSAHLGWREVPLSKMIISCLPTDLPLIVSNRTIAATLGEMWFGKGKEAQNFICINSGE
                     AVGAGIVIDGHIYRGLRDGAGEVGHVALLSGEKVCSCGKRGCIESAISLYSLMKELGE
                     EYQGEAAAASFLGEKKDDLRIQELLYNAFSLIGELSALLINVLAPEKIILTGEMARID
                     PDNLLRLVRKKVEEKALGLLADEVKIEVSNLNREQEILWGAALVMENIFSLPRLSKK"
     gene            complement(2439..2648)
                     /locus_tag="PKN31_07610"
                     /pseudo
     CDS             complement(2439..2648)
                     /locus_tag="PKN31_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2662..2802)
                     /locus_tag="PKN31_07615"
     CDS             complement(2662..2802)
                     /locus_tag="PKN31_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99823.1"
                     /translation="MAVNREFPQGVRGENLKLVNDNGSQPTSRSFMKDMVLLGIERLY
                     FL"
     gene            complement(3058..>3273)
                     /locus_tag="PKN31_07620"
     CDS             complement(3058..>3273)
                     /locus_tag="PKN31_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99824.1"
                     /translation="YTIKAACEALGIARSSYYASRKKQTTEKAERKVKEKDAEIIEKI
                     KEIKTLHPFWGYRRKLLAEAPGKHSYQ"
BASE COUNT          934 a          764 c          491 g         1084 t
ORIGIN      
        1 ttcttttctg ctgaagataa aagagaaaat acaaccatac taacccgata accatcttca
       61 aaaccagcta taaactcttg cttcgcatat agcctgttaa caaaatctct aatactttca
      121 tgggcaaaac cactagcttt tccatgaata agaggcataa ttaaaatgtc gggatgatca
      181 cttttatctt ctaagtatcg ttcaatagct tgattattag taaggtccat gttgatcatt
      241 cccttgcttc ccaagatatt aatttttata tcattccaat gggggttggt attagggata
      301 atccagttat tttctatagt agcaataact ccattttcaa attctaatat gctctgataa
      361 atatcaggaa cattgattcc tttttgaaca agcactttag agcgagatac tgaataaacc
      421 cgtgttattt cactattaga caaaaagcgc agggtatcta cagtatgact ccccaaaaac
      481 cacagaatgg aagatttttc tgcccaggat aacatttgag taggaacgta aacgatatca
      541 tttaagcgat aatacataga gattaactca cctaattctc ctttatctat gctctctttg
      601 gctatcacaa taggaggatt ccagcgagta tgaaagtcta ccattactcg agaactagat
      661 ttttggaaag catccttcat cgcctcaagg tcttctttgt tagtggccaa gggcttttct
      721 accagtatat cttttcccgc ttggcaagca gctacaaaag gttcccgatg ggcaaaatcg
      781 ggagtagtaa ttcctactgc ctggatatcg gggtctttta acagcttctc aatatcagta
      841 taccagcgct tagctccata cttttgtgcc aggtgacgag ctttttcctc cacaagatca
      901 catactgcaa taagttcaga ataaggttca ctagagaaaa ttcgagcgtg agcttctccc
      961 cacgtaccag caccaattac tccacaacca attttatcca tttcaatcct ccttcaaatt
     1021 aacttcactt tttacttaga cgaggcaaac taaaaatatt ttccattacc agggctgctc
     1081 cccaaagaat ttcctgttct ctattcaagt tactaacctc tatttttact tcatcagcca
     1141 ataatcctaa agctttttct tctacttttt ttctcaccaa tctaaggaga ttatccggat
     1201 cgattcgagc catttctcca gttaaaatta tcttttctgg agcaagaaca ttaattaaaa
     1261 gtgctgataa ttctccaatc aaggaaaaag cattatagag aagctcttga atcctaagat
     1321 catctttttt ttctcccaaa aaactagcag ctgccgcctc tccctgatat tcttctccca
     1381 actctttcat taaagagtaa agcgatatag ccgattctat gcatcccctt tttccacaag
     1441 agcaaacttt ttcccctgaa agtagggcaa catgccctac ttcccctgct ccatctctta
     1501 atcctcggta aatgtgacca tcgattacta tccctgctcc tacggcttct ccgctattaa
     1561 tacatataaa attttgcgct tctttccctt ttccaaacca catttcccct aaagtagcgg
     1621 ctatggtacg gttactaact attaagggta gatctgttgg aaggcaagaa ataatcattt
     1681 tactcaatgg aacttctctc catcctagat gcgcagaata agaaattgtc ccttgtttat
     1741 gatcgacgat cccaggaact cccaacccta tacccaaaaa acaattctca gggtaagaag
     1801 aaagaaaaaa acgaagaaaa taagatatct tagttactgt ttctttagca tcttcttttg
     1861 aggagagata gaaatgccta ttatctagta ctgttcctaa taaatttatc actgctacct
     1921 ctccctctcc tccatctttc aaaaaaacac ttaaaattaa gggaccataa ggattgaggg
     1981 ataaaagttc cgatcttctt cctccagaag agtttcccca cccactctcc acaataagcc
     2041 cctcttctat caactctcga acaattccgg aaacagtaga tttgctgaga ccaaaggctt
     2101 ttccgatctg agaacgagaa actttcccct cttcccaaat aaagcggaag atcattttct
     2161 tatgtatgtc attaattttg gagctactga gagtaaccat attttatttg tttgttcgac
     2221 ccgggaacga acttttccaa aattctagac cttcctactc tttttgtcaa tgcctcatgt
     2281 agttattgta gttattctgt gataatgtcg cccttaaaat tctcgtgaaa atgtcccttt
     2341 agtaataaaa tggaattacc tatgactcaa aaagaaagaa ataaactata atgcgcccca
     2401 taagtcaaga catattttac cctccaaaag tatagaggtc acgcagcaca ctctccttga
     2461 agttcatagt attgtttctc aaactctcga ggagatttat atcccagttt agaatgggga
     2521 tagaattggt tgtaatcttc ttctatccat tgaccaataa cctcttgggc ttcttctaag
     2581 ctggtaaatt cattaatcca aatcacttct tcttttatgg ttctcatcat cctctctgtt
     2641 tccgcattac ctttggggtt atcataggaa gtacaatctc tctattccca aaagcaccat
     2701 atctttcata aatgatctgc tggtgggttg agagccatta tcgttgacca gtttcaagtt
     2761 ctctcctctt accccctggg gaaattctct atttactgcc atatggaggg ctcttttcca
     2821 ctcctcagct ctaccgcgag agcaaactca gcctactatt ttcttggtat accagtcaat
     2881 tactatgact aaatagaccc accctaaatc agataccatg aatttagtca tgtctattcc
     2941 ccaaatctca tgtggtcttt ctgccctcct tttctcctct ggggagttct tttagctttg
     3001 tacttcttag cttccatcat tagattttct tccttcataa tccttcttac tcgtttctca
     3061 ttgataacta tgttttcccg gtgcctcagc caagagtttt cttctgtatc cccagaaagg
     3121 atgaagagtc ttgatttctt ttatcttctc tattatctct gcatcttttt ctttaacttt
     3181 tctttctgct ttttcagttg tctgtttttt ccgagatgcg taataactgc ttcttgctat
     3241 acccagagct tcacaggcag ctttaattgt ata
//
LOCUS       DAOPSS010000155         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_24525_length_3154_cov_7.059374, whole genome shotgun sequence.
ACCESSION   DAOPSS010000155 DAOPSS010000000
VERSION     DAOPSS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24525_length_3154_cov_7.059374"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            833..1465
                     /gene="nth"
                     /locus_tag="PKN31_07625"
     CDS             833..1465
                     /gene="nth"
                     /locus_tag="PKN31_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOA99825.1"
                     /translation="MEEKERVARILDTLEEHFPDARLLLEFNNPFELLVATILAAQAP
                     DERVNQVTRTLFQRFPTPQALARASLSEIEEVIKSLPYYRQKARFLKESSSILVEKFQ
                     GEVPPDLSELTSLPGVGRKTASIVLANAFNIPAIAVDTHVARVSQRLGLASSSSPSKI
                     EKDLASIIPPERWIRAAHLLGFLGRTTCLARKPRCPECPVREWCPSAASL"
     gene            complement(1431..2903)
                     /locus_tag="PKN31_07630"
     CDS             complement(1431..2903)
                     /locus_tag="PKN31_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99826.1"
                     /translation="MAFIQGRILLPSDTPWARKAAREYMHFEKEEEKTKKLLAPLPDT
                     EIKAFLLESPDLITRTKSNEEGEFFLRVPPGENYFLEFYYQSQLVALAFANSLEEEVD
                     LGTVDSLVTAQALKIKMGEKGSSVSVSELAKNIEQMWARGEGLEKLEAVSNSPVSDPF
                     HLIEYFDFSFLPDGKTLIVNWKSRQPVVARLYYRSFNSSRYHKLTAKHKKEGAFSFGV
                     REFEGYLFYLEMENDQHLLGRTPLRAVRAPLSPEKRKIIIEGREDGPITVARRKVSGE
                     RTISFPKENRDLKLSLYRAMEREFEAEMYFNFLGVEEMPRFLVREGFEELEFAIYFPM
                     EFSFLWGPVGSKPGVSISAEDREKILELWRDEELALPVGRLMEIGYSTPFVEVVAYRG
                     GEYFHLFSNTDLKEKIFLFSLRRLDSLDLEAIIYYWGKLTLHGEGMFEDYSLELDLRG
                     RFEEGKEPLLWTFQKGEVEGEIKLWIDLEVTRKQRKGTTL"
BASE COUNT          805 a          789 c          606 g          954 t
ORIGIN      
        1 cagaggtttt cactgaggga tgttctctcc cctcagtggt ctttgccttt tagtttttgt
       61 cttttttggg gttataagga ggggtatttt ctcccctata gccggattta taagggggga
      121 aacattcccc ccttatactg aggaaggcgt cttcccttcc tcagttgtat ttgttttttt
      181 tgccttttgc ctttttgttt ttagtctttt tgggggttaa taagggggca gcgccccctt
      241 atagtcgata gaggcattca ggggtgacga cacggagtct ttttcctccg tgttattttt
      301 gatctttttc tcttgtcttt tttgtttttt ctctttcggg ggttataagg ggggatttat
      361 ccccttatag ttctgtggca atcccgagtt tttaacccca tatagcttgc tctataaaat
      421 ggcaaaaaag acaccctcac cctactctct cactccgagg gagagggaaa aatacataga
      481 ggttttcccg ttctattatt tagattcctg atagaaccac tcaggaatga cggacacgga
      541 gatattccct cttatactga gggacgtttt cttcctcagt agtatttgtt tttttatgct
      601 tttattgttt tcggtttacg ccgcaggctg cccttttaat cttacgccgt agaccgcctg
      661 ttttctcttt atttaagtct ttagctttgt cttttcaatt ctccaagagg ttggttacag
      721 ggtgaccagt tttcccttta tagttctctc cttttctctt ctttcaattt ctattataat
      781 ttactcaaaa tatctttgct ctttttgact agagctcaga aagaaggaca aaatggagga
      841 aaaagaaaga gtagcccgta ttctggatac tttagaagag cactttcccg acgctcggtt
      901 gcttttagaa tttaacaatc cttttgagct tttagtggcc accattttag ctgctcaagc
      961 tcctgatgag cgggtaaatc aggtaaccag aactcttttt caacgtttcc ccacacctca
     1021 ggctctcgct cgtgcctctc tctccgaaat tgaagaggtc atcaagtctc ttccttacta
     1081 ccggcaaaaa gcccgcttcc tcaaagaatc gagctccatt ttagtggaaa aattccaagg
     1141 ggaagttccc cccgaccttt cggagctcac ctctctgccc ggagtgggga ggaaaactgc
     1201 cagcatcgtt ttagccaatg ccttcaatat tccggctatc gccgtagaca cccacgtggc
     1261 tcgcgtatcc cagagattgg gtcttgcttc ttcctcttcc ccttctaaaa tagaaaaaga
     1321 tttagcctcg ataatccccc cggagcgctg gataagagcg gctcatcttt taggtttttt
     1381 aggtagaacc acctgtttgg ccagaaaacc tcgctgccca gagtgtccgg tcagagagtg
     1441 gtgcccttcc gctgcttcct tgtaacctct aagtctatcc acaactttat ttctccttcc
     1501 acttcacctt tttggaaggt ccagaggaga ggttctttgc cttcttcaaa cctaccccgg
     1561 aggtctaact ccagggaata atcttcaaac atcccttccc cgtgaagggt cagttttccc
     1621 cagtaataaa taatagcctc cagatccaga gaatccagcc ggcgcagaga aaaaagaaaa
     1681 atcttctcct tcaagtcagt gttagaaaaa agatgaaaat actctcctcc ccggtaagcc
     1741 accacttcta caaaaggagt gctatagcct atctccatca atctccccac agggagagca
     1801 agctcctcat ctctccacaa ttctaaaatt ttctcccggt cttctgcaga tatagaaact
     1861 cccggcttgc ttcccaccgg cccccacaga aaagaaaatt ccatgggaaa ataaatagca
     1921 aactccagct cttcaaatcc ctctcgcacc aaaaaccggg gcatttcttc cactcctaaa
     1981 aaattaaaat acatttctgc ctcaaactct ctctccattg cccggtagag agagagtttg
     2041 aggtctctgt tttctttagg gaaagaaatt gtcctttctc ctgagacttt acgacgagcc
     2101 acggtgatgg ggccatcttc tctcccctct ataattattt ttcttttttc tggagaaaga
     2161 ggggcgcgca ctgcccggag aggagttctc cccagaagat gttgatcgtt ttccatctct
     2221 aaataaaaaa gatatccttc aaactctcgc actccgaagg aaaacgctcc ttcctttttg
     2281 tgcttggcgg ttaacttgtg atagcgagaa gaattaaaag aacgataata aagacgagct
     2341 accacgggct ggcgagattt ccagttcact atcagggttt tcccatcagg aagaaaagaa
     2401 aaatcaaaat attctatcaa gtgaaaaggg tcagaaacag gagagttgct caccgcctcg
     2461 agcttttcta acccctcacc tctggcccac atctgctcta tgtttttagc aagctcagag
     2521 actgaaacag aacttccctt ctctcccatc tttattttca aagcctgagc ggtaacaagg
     2581 ctatcaacag tgcccaaatc tacctcctct tccagggagt tagcaaaagc cagtgccacc
     2641 agttgagact ggtaataaaa ctccagaaaa taattttctc caggaggaac ccgtagaaaa
     2701 aactccccct cctcgttgct tttagtccgg gttattaaat cgggactttc aagaagaaaa
     2761 gcttttatct ccgtatccgg aagaggagcc agaagctttt ttgttttttc ttcttctttc
     2821 tcaaaatgca tatattcccg ggccgccttt ctcgcccagg gtgtatcaga aggaaggaga
     2881 atcctcccct gaataaaagc caaaataacc acctctcatt caataaactg tatcttttga
     2941 aaaatctttt tagaataata ccacaaacta aattatatca cccttctcct tttccgtcat
     3001 cgcgagggtc gctacctcgc tggcatagag agggaaagaa tatataatat agaatctccc
     3061 cgaaagggct tccccctccg tcatgcctga aatttaggcg ggaatccaaa ggcttttgtc
     3121 cttaaataat ttagattcct gatagaggca ttca
//
LOCUS       DAOPSS010000156         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_24634_length_3143_cov_7.265220, whole genome shotgun sequence.
ACCESSION   DAOPSS010000156 DAOPSS010000000
VERSION     DAOPSS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24634_length_3143_cov_7.265220"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKN31_07635"
     CDS             <1..269
                     /locus_tag="PKN31_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOA99827.1"
                     /translation="PFYMAFPLSSVDFSIREGREIPIEERDPEEVIAPRGIKITPPGV
                     GVRNPAFDVTPHHLITALITEKGIVYPPFEEVLLNWKTEARQNG"
     gene            262..1419
                     /locus_tag="PKN31_07640"
     CDS             262..1419
                     /locus_tag="PKN31_07640"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99828.1"
                     /translation="MDNNIITIEDVRKLLEFKPEEALVTSFYLNVDPRHVPASKFTTL
                     TRNLLREKQQSLEKEDWPQGLLDSVREDFKKIENFVVNHFELKGRVRGLVIIADAAHD
                     FFQVYRIAQPVKSRVVVDPNPYIRPLVAILDEYHRLMLVLLDQREARLLEVYMGEILE
                     HKAYYSDVRGKVKEGGWYGLEERRIERSIKNKVIQHYQRVADAVLEHFRVGHFEYLFI
                     GMRAEDYSLFLPLLHSYLQPRVKGRLDIGPKDHINTIIEAALKAEREIEEEEENKILE
                     NLVETLNRGGLAIKGIDNVLRSIPLGACQVLVVDDDYHMPGYVCKDCAYLALEEGNCA
                     FCGKEALPVEDVIEEAMEEVLLQRGEIKHIATDNPRLEEISHIGAFLRYKI"
     gene            1513..1677
                     /locus_tag="PKN31_07645"
     CDS             1513..1677
                     /locus_tag="PKN31_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99829.1"
                     /translation="MRSTGAHVLAYLQLKEYLGMEGEIKLGWRFSTRCRLHTAKDMDP
                     RKLKEEFGRD"
     gene            1696..1854
                     /locus_tag="PKN31_07650"
     CDS             1696..1854
                     /locus_tag="PKN31_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99830.1"
                     /translation="MGTRGVLLYGVEEETRSPVRERIDILERGGGFLFNRVHDILPDV
                     LPSNVVAA"
     gene            1887..>3143
                     /locus_tag="PKN31_07655"
     CDS             1887..>3143
                     /locus_tag="PKN31_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOA99831.1"
                     /translation="MMERYCLVLDCGATSLRAVAVSEKGEILHLSSFPNAPSPQEGEK
                     DFLIWDVDEIWRKFTLAIRQVIAEAGKEIFAVTVTTFGADGAPVRRDGTLTYPVISWQ
                     CQRTAPQAKEITNFLSPREIFRITGYQIIPFNTLFKLLWLRNNAPRSLDEADTFLMMP
                     GILSFKLCGEASMDFTSASTTMMLDIERKCWSEPLLSLAGLTPSFLPPLQHPGEVIGK
                     VQAKASEETGIPQGTPVVVTGHDTQFAIVGSLASPEELVLSSGTWEISALRLPYYRDS
                     SSFFERGGLTELDAEENLWNPQMLMIAGGVVEWVRRNFFSDIKDEKNIYEVMVESARS
                     IPPGAGGIVVIPAFMPSGPTKPYDTQGTILGLELTSDRGQVFRAVLEGLSFQLKEAVL
                     ALQEAFGYTPKRVRVVGGGAKNKLWNQ"
BASE COUNT          880 a          557 c          804 g          902 t
ORIGIN      
        1 taccttttta tatggctttt cctttatcca gtgtagattt ctctataaga gaaggtaggg
       61 aaatccccat tgaagaaaga gacccggaag aggtaattgc tcctcgaggg ataaaaatta
      121 ctcctcccgg ggtaggagta agaaaccctg cctttgatgt cactcctcat catttaatta
      181 ctgcacttat taccgagaaa ggtattgttt atcctccttt tgaggaggtg ttactgaatt
      241 ggaaaacgga ggcaagacaa aatggataat aatataatta ccatagaaga tgttagaaaa
      301 cttttagaat ttaaacccga ggaggctttg gtcacttcct tttatcttaa tgtggacccc
      361 cgccatgttc ctgctagcaa atttaccact ttaactcgta atttgcttcg ggagaaacaa
      421 caatctttag agaaagaaga ttggccgcag gggctgttag atagtgtaag agaagatttc
      481 aagaaaatcg agaattttgt tgtcaatcat tttgagttaa agggaagagt tagaggactg
      541 gtaataatag ctgatgcagc acatgatttt ttccaggtct atcgtattgc tcaaccggtt
      601 aaatctcggg tagtggtgga ccctaatcct tatattcgtc ccctggtagc gattttggat
      661 gaataccatc ggctgatgct ggttcttctc gatcaacggg aagctcgttt gttagaagtc
      721 tatatgggag aaatattaga gcataaagct tattactccg atgttcgagg gaaagtaaaa
      781 gagggaggat ggtatgggtt agaagagcgc cggattgaga gaagtattaa aaataaagtc
      841 atacagcatt accagagagt agctgatgct gttctggaac actttagagt ggggcacttt
      901 gagtatcttt ttataggtat gagagcagaa gattactctc tttttcttcc tcttctccac
      961 agctatctgc aacccagagt gaaaggaagg ttggatatcg gtcctaaaga ccacattaat
     1021 accataatcg aagcggcact aaaagcagag agggaaatag aagaagagga agaaaacaag
     1081 attctggaaa atcttgtgga gaccttgaat aggggaggac tggctatcaa agggatagat
     1141 aatgttttgc ggtccatacc tctcggggct tgccaggttt tggtggtaga tgatgattat
     1201 catatgccag ggtatgtgtg taaagattgt gcctatttag ctctggaaga gggaaattgt
     1261 gccttttgtg gaaaagaagc tcttccggtg gaagatgtga tagaagaagc aatggaagaa
     1321 gttcttttac aaagagggga gattaaacat attgctactg ataacccccg attggaagag
     1381 atctctcaca ttggagcttt tctgcgatac aaaatttgag gaaggaaaga tgagggtagc
     1441 ttgacttatc gggacatagc gcttttgacg ctgagtggcg aggaaggata gattcctgtt
     1501 aattttggaa caatgcgctc taccggtgcc catgttctgg cttatctcca gctaaaagaa
     1561 tatctgggaa tggaagggga gataaaattg gggtggaggt tctcaacccg gtgcagacta
     1621 catacagcta aagatatgga ccctcgcaaa ctaaaagaag agtttggtag ggattaggta
     1681 ttctgggaaa aagagatggg aacccgggga gtacttcttt atggtgtcga agaggaaaca
     1741 agaagcccgg tgagagaaag aatagatatt ttagagcgag ggggaggatt tctctttaat
     1801 cgggtgcatg atattttacc tgatgttctc ccttctaatg tagtggctgc ctgaaagtac
     1861 ggaatatatt ctctttgagg aggaaaatga tggaaagata ttgtttggtt ctggattgtg
     1921 gagctaccag tcttcgggcg gtagcagtga gtgaaaaagg agaaatactg catctttctt
     1981 cttttcccaa tgccccttct cctcaggaag gagaaaaaga ttttcttatc tgggatgtag
     2041 atgaaatctg gagaaaattt actctggcca ttcggcaggt tattgctgaa gcaggaaaag
     2101 agatttttgc tgttacagtt actacttttg gagcagacgg agcgcccgtc cggagagatg
     2161 ggactctgac ttatccagtt atttcctggc agtgtcagag gactgctcct caggctaaag
     2221 aaatcaccaa ctttcttagc cctcgggaga tttttcgcat tactggatat cagataatac
     2281 cttttaacac cctgttcaag cttctttggc tgcgaaacaa tgctcctcgt tctctggatg
     2341 aagctgatac ttttttgatg atgccgggta tcctgagttt caagctttgt ggagaagcaa
     2401 gcatggattt tacctctgcc tccactacta tgatgctgga catagagaga aaatgctggt
     2461 ctgaaccttt gctttctctg gcaggtctaa caccttcttt tttgcctcct cttcaacatc
     2521 ctggagaggt aattggaaaa gttcaggcta aagcaagcga agaaaccgga attcctcaag
     2581 gtacgccagt ggtcgtaacc gggcatgata cacagtttgc catagtgggt tctttagctt
     2641 ctcccgaaga gctggtattg agttctggca cctgggagat ttccgcgctg cgccttcctt
     2701 attatcggga ttcttcttct ttttttgagc ggggaggctt aacagagctg gatgctgaag
     2761 agaacttgtg gaatccccag atgcttatga tagccggggg agtggtggaa tgggtgagga
     2821 ggaatttttt ctccgatatc aaggatgaga aaaatatcta tgaagtaatg gtagagtctg
     2881 ctcgctctat tcctcctggt gcaggaggca tagtggtaat tcctgctttt atgccttctg
     2941 gccctaccaa accttacgac actcaaggta ctattttggg attggaattg acctctgatc
     3001 gggggcaggt tttccgtgct gtattagaag gtttatcttt tcaattaaaa gaagccgttt
     3061 tagctcttca ggaagctttt ggttataccc ctaaaagggt gcgagtagtg ggaggaggag
     3121 ctaaaaacaa gctgtggaat caa
//
LOCUS       DAOPSS010000157         3053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_25646_length_3053_cov_7.665777, whole genome shotgun sequence.
ACCESSION   DAOPSS010000157 DAOPSS010000000
VERSION     DAOPSS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25646_length_3053_cov_7.665777"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PKN31_07660"
     CDS             complement(<1..1321)
                     /locus_tag="PKN31_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99832.1"
                     /translation="MEIFWGKYAEEIESSFITLEEEKVISRLWEKDYTLWKPYPQEIV
                     NRLGWLSAPQEMQKNVAQLEDMTAGLRKEGFQEALLLGMGGSSLAPDLFQKVWGNKEG
                     FLNLYVLDSTDPEMVNHYAQSLDPGKTLYLVSTKSGNTLETLSFFKFFYNLAQKKLGK
                     EAGKHFIAITDPGSPLVQLGERYDFRRVFLNPPDIGGRYSAFSFFGLVPAALLGIDLS
                     LLLEKATIASQKCSPSTPLKDNEGALLGTTLGILAQKGRDKLTFFFSSPRWESFGDWL
                     EQLVAESTGKEGKGILPVIDRQPGKPEVYGEDRLFVYIEGEKNDSLNNLLEELKNAGH
                     PVIKISVASHYDLGEQMFIWEFATALSGYWLKINPFDQPDVESTKKFTQEMMRRYKEG
                     SFEEENPVLVEKGFNIFCDFSASSLKEVLEQFLGFVEPGGYISIHAYL"
     gene            complement(1400..1876)
                     /locus_tag="PKN31_07665"
     CDS             complement(1400..1876)
                     /locus_tag="PKN31_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOA99833.1"
                     /translation="MEKRFFLCKLFEKNEGFTLIEVLISSAIFLSLVVAICSLLVPWG
                     RVFNSLSQKIEEEENLLGSVNTLFQLVRYSNNLQSSSDGKELSMNLSSNPLKIYSQGG
                     SLLLKNKGVANPVAEGCSEACFTVEGSKEKPLVSAHLVFGKEVLDIKINSCLLLSP"
     gene            complement(1833..2171)
                     /locus_tag="PKN31_07670"
     CDS             complement(1833..2171)
                     /locus_tag="PKN31_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOA99834.1"
                     /translation="MKNDEGFILISVLIALGIFSLLVLAIGQEINLMGKVGRSSALKA
                     KALVDAVNTMEALKQEEKPVSGSEEKEGFIIESKVKEFSFSSQIYEIKVTVEDKNGKE
                     ILSLQTLREK"
     gene            complement(2161..2610)
                     /locus_tag="PKN31_07675"
     CDS             complement(2161..2610)
                     /locus_tag="PKN31_07675"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HOA99835.1"
                     /translation="MEINPEKFSSGFTILELLIVISIISIISAFSFPHLLNWQTSLLG
                     RLTAQRIASDLRLAKIEALRTGATTKITFDPAQDLFIYRGCNSQPKIVNFPKGVDLYT
                     TNFSQHTISFYPTGTPSMGGTITIRVNGKLMYLTITPVTGRVRIVEK"
BASE COUNT          923 a          609 c          539 g          982 t
ORIGIN      
        1 gaaggtaagc gtgaatagaa atatagcctc ctggctcaac aaaccccaaa aattgttcta
       61 gaacctcctt caaggaagaa gcagagaaat cacaaaatat attgaatcct ttttccacca
      121 gaacagggtt ttcttcctcg aagctacctt ctttgtatct tctcatcatt tcttgagtaa
      181 attttttggt agattctaca tcaggctgat caaaaggatt tatttttaac cagtagccgg
      241 ataaggcagt ggcaaattcc cagatgaaca tttgttctcc cagatcgtag tggctcgcaa
      301 cagatatctt aatcactgga tgcccggcat tttttagctc ctcaagtaaa ttattgagag
      361 agtcattttt ttctccttct atatagacaa ataggcggtc ttctccatat acctctggtt
      421 ttccgggttg cctatcgatg accggcaata ttcctttccc ttctttccct gtactttcag
      481 caaccaattg ttctaaccaa tcaccaaaac tttcccatcg aggagaagag aagaaaaaag
      541 ttagtttgtc tcttcctttt tgggctaaaa ttcctaaagt ggttcccaga agggcacctt
      601 cattatcctt aagaggggta gagggagaac atttttggga ggcaatagtc gctttttcta
      661 aaagaagtga taaatctatt cctaaaagag cggcaggtac aagaccaaaa aaagaaaagg
      721 cagaataacg acctccaata tcaggaggat tcaaaaacac cctacggaag tcatatcgtt
      781 ctcctaactg aactagagga ctaccagggt cagttatagc gataaaatgt tttcctgctt
      841 ctttcccgag ttttttctgt gccagattat agaaaaactt aaaaaaggag agagtttcca
      901 gagtgtttcc tgatttggta gagacgagat aaagagtttt gccaggatct aaagattgag
      961 cataatgatt taccatttca ggatcagtac tgtcaagaac atagagattc aaaaaccctt
     1021 ctttatttcc ccatactttc tgaaataagt caggagccaa acttgaccct cccatcccta
     1081 aaaggagagc ttcctgaaaa ccttcctttc tcagcccagc agtcatatct tctagttgag
     1141 ctacattttt ttgcatttct tgaggggcac ttaaccatcc caggcgattg acaatctcct
     1201 gaggatatgg tttccataga gtataatctt tctcccacag gcgagagatt accttttctt
     1261 cttccagagt gataaaagat gactctattt cctctgcgta ttttccccaa aatatttcca
     1321 ttctatttcc tcctttcaaa gctatcaata ggagaatagc aagataattt ttcctggtca
     1381 agaataaagt attttcaatt tacggtgata ataaaagaca agagtttatc ttaatatcta
     1441 ggacctcttt tccaaagact aaatgagcac tcaccagcgg tttttcttta cttccttcca
     1501 cagtaaagca ggcctcgcta catccctcag ctacaggatt ggctactcct ttgtttttga
     1561 gcaacaaact ccctccttgg gaatagattt tcaaggggtt agaagacaga ttcatactta
     1621 gctctttacc gtcagaggag gattgaagat tgttactata tctcaccagc tggaaaagag
     1681 tatttactga tccgagaagg ttttcttctt cctctatttt ttgagatagg gaattaaaga
     1741 ctcttcccca gggaacaagt aagctgcaaa tagctaccac taaagataga aaaatagcag
     1801 aacttattaa gacttcaata agagtgaagc cttcattttt ctcgaagagt ttgcaaagaa
     1861 agaatctctt ttccattttt gtcctccacc gttactttga tttcatagat ttgtgaagaa
     1921 aatgaaaatt cctttacctt actttctatt ataaaaccct ctttttcttc gcttccagaa
     1981 acaggctttt cttcttgttt taatgcttcc atagtattta cagcatctac aagagcttta
     2041 gccttaagtg cagagcttct ccccaccttt cccatgagat ttatctcttg cccaatagcc
     2101 agcaccaaaa gagaaaagat acctaaagca ataagaacgc ttattaaaat aaaaccttca
     2161 tcatttttca acgatacgca ctctcccggt aactggggtg atggtaagat acattagttt
     2221 tccattcacc cggatagtaa tggttcctcc catggaggga gtaccagtag ggtagaagga
     2281 aatagtatgt tgactgaagt tggtagtata gaggtctacc cctttaggaa aattaactat
     2341 ttttggttga gagttgcaac ctcgataaat aaaaagatcc tgggcgggat caaaggtgat
     2401 tttggtagtg gctccggttc gtaacgcttc tatcttagct aaacgtaaat cacttgctat
     2461 tctctgagca gtaagtctac ctaagaggga agtttgccag ttgagtaagt gaggaaaaga
     2521 aaaagctgaa attatcgaaa tgatggaaat tactattaag agttcgagga tagtaaaacc
     2581 tgatgagaat ttttccggat ttatttccat ttctttccct gtttctttta tttatggaac
     2641 atattatacg ccttttctaa gtttttccac aattggatgc tatattttag caaatttttg
     2701 atatttttct ggaggggcaa aaaattcgat aaagagcagg atagctctta ttaattgatt
     2761 ctgtcatttt cttaatgaga tgagaaggaa gtatctattc cctttgtttt tattccgcct
     2821 ctcatagatc aattaattat attttgacac cttttttaaa tcctgaagca caaaaatatt
     2881 tttttcaaaa ccctctctta gagggtgttg ggtaaaattt aaaattttac caaatcctgg
     2941 ctatttaagt tttaaaattt taccaaattt agatcggaat tcttaaccca acttgcggct
     3001 ttttcccaga aaaacctcct attttcaggt atacttgagg tatagatcct tga
//
LOCUS       DAOPSS010000158         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_25824_length_3036_cov_7.793693, whole genome shotgun sequence.
ACCESSION   DAOPSS010000158 DAOPSS010000000
VERSION     DAOPSS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25824_length_3036_cov_7.793693"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKN31_07680"
     CDS             <1..810
                     /locus_tag="PKN31_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOA99836.1"
                     /translation="QRYGGINAEDPVTNRLVEETKGLVLTYQNELASAVFHSESGGFT
                     ESALDVWGREVPYLVEVASPWEGNAPHSSWRVEFTKEELEESLREARLIQGEILDIRL
                     SSSSEGRVEEVEVITTDGEFSFPASRFREAIGWEKLPSTFFAMKKEEKEQKVRKETFP
                     SSSSSLSSSSFDYDEWMEKDWDLDDIINFLKMREEERNQKKGISPPPRIVVEEEVVSQ
                     GTAFIFEGKGYGHGVGLSQWGAKGMAEEGFSYEEILSHYFPGCRIERTTFK"
     gene            807..1838
                     /gene="queA"
                     /locus_tag="PKN31_07685"
     CDS             807..1838
                     /gene="queA"
                     /locus_tag="PKN31_07685"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOA99837.1"
                     /translation="MSSLVSEFDFSLPPELIAQEPFYPRDHCRLMVVDRERKTWEHRL
                     FYEVLEYLEPPDVLVINDTRVLPARFWVYKEETGGKVEILFLRHRGEDWECLLRPSRK
                     IKVGQILRLQKDSHFSWMVKGQNNGMWLVNPLFSPREEENLFQSFGEAPTPPYVKNRN
                     IRLEDYQTVYARIPGSVAAPTAGLHFTPRLLKKLKEKGVKVVSLTLHVGLGTFQPVKE
                     ERIEDHNMHSEYFVFTESAAQTILEAKEKGGKVVAVGTTVVRVLESIWQRSGTLKGME
                     GETNLFIYPGFHFQVIDWLITNFHLPRSTLFMLVCALAGTDFMKEAYEEAIKNNYRFF
                     SFGDAMLIK"
     gene            1849..2979
                     /gene="tgt"
                     /locus_tag="PKN31_07690"
     CDS             1849..2979
                     /gene="tgt"
                     /locus_tag="PKN31_07690"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOA99838.1"
                     /translation="MENFELLAVDYYSQARLGLLHTAHGVIETPVFMPVGTQGTVKAI
                     APDRLEEMGVKIFLCNTYHLHLRPGEDVVKEAGGLHHFISWPYSLLTDSGGFQVFSLT
                     DLVDVDEDGVYFRSHLDGSLHRFTPEVALQIQMDLGADIVVPLDQCIGFPASSHEERI
                     GAERTLQWAKRSKQFFSSPHQLLFGIIQGGMDEDLRIYCTKETVAMDFAGYALGGLSV
                     GEPPPLTYEIIEKVEPFLPHDRPRYLMGVGTPESIIEGVRRGIDMFDCVLPTRNARNG
                     CLLTHQGKVNIGRQEFSRDFRPVDAECHCYTCLNFSRAYLRHLFKCKEILASELATIH
                     NLYFMVELMENIRKSLREGYFLEFAEEFLAGYESNSSSFSKK"
BASE COUNT          800 a          550 c          787 g          899 t
ORIGIN      
        1 cagcgctacg gaggaattaa tgctgaagat ccggttacca atcgcctggt ggaagagact
       61 aaagggttag tacttactta ccagaacgag ttagcttcag ccgtttttca ttcagaaagt
      121 ggtggcttta ctgaaagcgc tcttgatgtg tgggggagag aggtgcccta tcttgtggaa
      181 gttgcttccc cctgggaagg gaatgcccct cactcatcct ggagagtgga atttacaaaa
      241 gaagaactgg aagaaagcct gagggaagct cgcctcatcc agggagaaat tctggatatt
      301 cgtttatcct cttcctcgga ggggagagtg gaagaagttg aagtgataac tacagatggt
      361 gagttttcct ttcctgcctc ccggtttcga gaagctatag gctgggaaaa gctccccagc
      421 actttttttg caatgaaaaa agaggaaaaa gagcaaaaag tgagaaaaga aacttttcct
      481 tcttcgtcat catcattgtc atcttcttct tttgactatg atgaatggat ggagaaagat
      541 tgggacttag atgatataat caattttttg aaaatgagag aagaagaaag aaaccagaaa
      601 aaaggtattt ctcctcctcc ccggatagtg gtggaggaag aagttgtttc tcaaggtact
      661 gcttttatat ttgagggaaa aggttacggt catggagtgg gtctttctca gtggggagca
      721 aaaggtatgg cggaggaagg tttttcttac gaagagatac tcagccatta ttttcctggt
      781 tgccggatag agaggacgac ttttaagtga gtagtttagt ttccgaattt gatttttctc
      841 tgcctccaga gctgattgct caggagcctt tttatcctcg agaccattgc cgattaatgg
      901 tggtggatag agagcgaaaa acgtgggagc accgcctttt ctatgaggta ctggaatatt
      961 tagagcctcc cgatgttctc gtgataaatg atacccgggt tttgcctgct cgtttctggg
     1021 tgtataagga agaaaccggg ggaaaggtgg aaattttatt tctgcgccac cggggagagg
     1081 actgggagtg tcttttgcgt ccttctcgaa aaataaaggt gggtcagatt ctcagattac
     1141 aaaaagactc tcattttagc tggatggtga aagggcagaa taacggaatg tggttggtta
     1201 accctctttt ttctccccga gaagaagaga atttattcca gtctttcggt gaagctccta
     1261 ctcctcctta tgttaagaat cggaatataa gattagagga ttatcaaacg gtttatgctc
     1321 gcattcccgg gtcagtagct gctcctactg ctggtttgca ctttactcct cgtttgttga
     1381 aaaaattaaa agaaaaagga gtaaaggtgg tgtctcttac tcttcacgtg ggattgggga
     1441 cttttcaacc ggttaaagaa gagagaatag aagaccacaa tatgcactcg gagtattttg
     1501 tgttcacaga aagcgcggca caaactattc tcgaagctaa agaaaaggga ggcaaagtag
     1561 tagcagtggg aacaacggtg gtgagagttc tggagtcaat ctggcagcgg tctggcacct
     1621 taaagggcat ggaaggagag acgaatctct ttatctatcc tggttttcat tttcaggtga
     1681 tagactggct gattaccaat tttcatctcc ctcgctccac cctttttatg ctggtttgcg
     1741 ctttagccgg aaccgacttt atgaaggaag cttacgaaga ggctatcaaa aacaattatc
     1801 gcttttttag ctttggcgat gctatgctca taaaataaga ggtgaagttt ggaaaatttt
     1861 gagcttttag ctgttgacta ttattcccaa gctcgtctgg ggctattaca taccgcccat
     1921 ggagtaatag aaacaccggt gtttatgccg gtaggaactc aggggacggt gaaagctatt
     1981 gctccagatc gcttggaaga aatgggagtg aagatttttc tctgcaacac ttatcatctc
     2041 catctccgac cgggagaaga tgtggtaaag gaagcagggg gtttgcatca ctttatttcc
     2101 tggccttatt ctcttttaac tgatagtggt ggttttcagg tgtttagttt gactgatctc
     2161 gtggatgtgg acgaagatgg agtgtatttt cgctctcact tagatggatc tctccaccgt
     2221 ttcactcctg aggtagcttt gcaaattcag atggatctgg gagcagatat tgtggtacct
     2281 ctggaccagt gcattggctt tccagcttct tctcatgagg agcgcatcgg agcagagagg
     2341 actcttcagt gggcaaagag aagcaaacag tttttttctt ctcctcatca gctccttttc
     2401 ggtataatcc agggagggat ggatgaggat ttacggattt attgcactaa agagacggtg
     2461 gcgatggatt tcgctggata tgctctggga ggtttgagtg tgggtgaacc ccctcctctc
     2521 acttatgaga taattgaaaa agtggaaccc tttttacctc atgataggcc tcgttatttg
     2581 atgggggtgg gcacaccaga gagcataata gagggagtgc gccggggtat agatatgttt
     2641 gattgtgttc ttcctactcg caatgcccga aatggttgcc ttttaactca tcagggcaaa
     2701 gttaatattg gtcggcagga gttttctcgg gattttcgtc ccgtagatgc agagtgccat
     2761 tgctatacct gtcttaattt ttctcgagct tacctgcggc atctgtttaa atgtaaggaa
     2821 atactggcca gcgagttggc tactatacat aatttatatt ttatggtaga gttgatggaa
     2881 aatattagga aaagtttaag agagggttat tttctcgaat ttgccgagga gtttctggcg
     2941 ggatatgaaa gtaattcctc ttctttttct aaaaaataaa gaaggagtga ttgatgatga
     3001 actggtttat ttctctggct cacgctgcta atgaag
//
LOCUS       DAOPSS010000159         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_26185_length_3008_cov_9.644429, whole genome shotgun sequence.
ACCESSION   DAOPSS010000159 DAOPSS010000000
VERSION     DAOPSS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26185_length_3008_cov_9.644429"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="ligA"
                     /locus_tag="PKN31_07695"
     CDS             complement(<1..1073)
                     /gene="ligA"
                     /locus_tag="PKN31_07695"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOA99839.1"
                     /translation="MEDIEKIRSEIEKLREIIRHHDYCYYVLNSPEISDEEYDALFRK
                     LQELEAKYPELVTPDSPTQRVAGQPQEAFAPFFHSRPLLSLNNAFTPEEVRDFDRRVK
                     RMLKVESLDYVVELKIDGLAVNLRYENGIFNRGATRGDGTVGEDVTLNLKTIHSIPLR
                     LRGDRIPRVIEVQGEVFMRKKDLEKLNEERKQKGEPLFANARNAAAGSLRQLDPRITA
                     ERKLDIFNYGAFLIDSDYYPSTHWELLNYLKELGFKINPHVQLISDIEEAIRIHEKWE
                     KERKNLDYDIDGLVIKVNPLSYHEILGATSRSPRWAIAYKFESTYAVTQVLDIEVNVG
                     RTGILTPVAILEPVNVGGAIVKRA"
     gene            complement(1084..2376)
                     /locus_tag="PKN31_07700"
     CDS             complement(1084..2376)
                     /locus_tag="PKN31_07700"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOA99840.1"
                     /translation="MAVSIVVGTHWGDEGKGKIVDYLGENAQVVARAQGGNNAGHTVI
                     VEGEKYVFHLLPSGVLHPGKVGILGDGMVVDPSSLLGEIDFLRQKGKDVSERLFLSSK
                     AHLVLPYHRILDGIYENIRGGRKLGTTGKGIGPAYEDKVSRWGIRVGDLEDEKLFAQK
                     LRVALDYKNLLLERAFNQPPLPYDKILEDYLGYASLLRPYIADTSKMIYQFYREGKNI
                     LIEGAQGTMLDLDHGTYPFVTSSYPVAAGSLLGVGLGLGSISNLEVLGVCKAYTTRVG
                     EGPFPTELREEMGDYLRDKGGEYGATTGRPRRCGWLDGVILRYAARINGITSLALTKL
                     DVLGGIDSLKVCRAYLVEGEEVDDFPVSSHLWDKCEPVYEEMEGWEEDISSVSSYYDL
                     PSSAQRFVEFIEDFMQIPIAIVSVGKERRNTLVRKEIF"
     gene            complement(2389..>3008)
                     /locus_tag="PKN31_07705"
     CDS             complement(2389..>3008)
                     /locus_tag="PKN31_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOA99841.1"
                     /translation="EEEAKKLGDVSYLLQGTTYPDVIESISIYGPSVRIKTHHNVGGL
                     PEHMQLKVIEPLRFLFKDEVRKLAVELGIPEEIVWRQPFPGPGLAVRIIGEVTEDRLQ
                     ILRDMDFIINQELRKSPIYKKLWQSFGVLVPVRSVGVVGDERTYKYVGVLRAVMSEDG
                     MTADWARISYDTMDAIARRVVNEVAGIGRMVYDITSKPPATIEWE"
BASE COUNT          807 a          811 c          525 g          865 t
ORIGIN      
        1 gctctcttta ctatcgctcc ccctacattt accggctcca gtatcgccac cggagtaaga
       61 atccccgtcc gacctacgtt tacttcaata tccagaacct gagttacggc gtaagtggac
      121 tcaaatttat aagctatagc ccaccgaggg cttcggctgg tcgcccctaa aatctcgtga
      181 taggatagag ggtttacttt tatgaccagg ccatcgatat cgtagtctaa attttttctt
      241 tctttttccc atttttcatg aatgcggatg gcttcctcga tatcagaaat cagctgaaca
      301 tggggattga ttttaaatcc cagttcctta agatagttca atagttccca gtgagtggag
      361 gggtagtagt cgctatcaat caagaaagca ccgtaattga aaatatccag ttttctttct
      421 gctgttatcc gggggtctaa ttgccgtaaa gacccggctg ctgcattacg agcgttggca
      481 aaaagaggct ctcctttctg ctttctttcc tcgttcaatt tctccaggtc cttttttctc
      541 atgaatacct ccccctgcac ctctattacc cggggtattc tatcccctcg caagcgaaga
      601 ggaatactat gaatagtttt aaggttgaga gtaacatctt ctcctactgt tccatctccc
      661 cgggtagccc cccggttgaa tatcccattt tcatagcgca aattgactgc taaaccatca
      721 attttaagct ctaccacata atccagactc tctaccttaa gcatcctctt cactcgtcta
      781 tcgaaatccc gcacctcctc gggagtgaaa gcgttattca aactgagaag gggtcgagag
      841 tggaaaaaag gagcaaaggc ttcctgcggc tggccagcta ctctctgagt cggagaatca
      901 ggagtaacga gttccggata ctttgcctcg agctcctgca gttttctaaa gagagcatcg
      961 tattcttcat cgcttatttc cgggctattt aaaacatagt aacaataatc atgatgacga
     1021 atgatttcgc gaagtttttc tatctcactt cttatttttt ctatatcttc cataaaggac
     1081 tttttaaaaa atctcctttc tcaccagagt gttccgccgc tccttcccta ctgaaactat
     1141 agcgatggga atctgcataa aatcttcaat gaattccaca aatcgctgag ccgaagaagg
     1201 taaatcgtaa taagaagaaa ccgaagaaat atcttcttcc catccttcca tctcttcata
     1261 aactggctca cacttatccc acaagtgaga acttaccgga aaatcatcca cttcttctcc
     1321 ttctactaaa taagctctgc atactttgag ggagtctatc ccccctaaaa catccagttt
     1381 ggttaaagct aaagaagtta taccatttat cctcgcggca taacgcaaaa tcactccatc
     1441 cagccaccca caacggcgag gacgaccggt ggtagccccg tattctcccc ctttgtctcg
     1501 cagataatct cccatctctt cccggagctc cgtaggaaat ggtccttctc ccactcgagt
     1561 agtataagct ttgcacaccc ctaacacttc cagattggaa atcgacccca accccagtcc
     1621 tactcccagt aatgatcctg ctgccacagg gtaggaagag gtaacaaagg gataagtccc
     1681 gtggtctaaa tctaacatag tgccctgcgc tccctcaatg aggatatttt taccttcccg
     1741 gtaaaactgg tagatcatct tggaagtatc ggcaatgtaa ggtcttaaaa gagacgcata
     1801 acctaaataa tcctctaaaa ttttatcata aggaagaggc ggctgattaa aagctctttc
     1861 taataaaaga tttttatagt ccagagctac ccttagcttt tgagcaaaaa gcttttcatc
     1921 ctctaagtcc cccactcgta ttccccaccg ggatactttg tcttcataag caggaccgat
     1981 gccttttcct gtagttccca gctttctccc accccgaata ttttcgtaaa ttccatctaa
     2041 aatccgatga tagggtaaaa ccagatgagc tttagagctc aaaaacaacc tctccgaaac
     2101 atctttccct ttctggcgca aaaagtcaat ttctcctaaa agagaggagg ggtcaacaac
     2161 catcccatct cccagtattc ccactttacc aggatgaaga acaccggaag ggagaagatg
     2221 gaaaacatat ttttctcctt ccactattac cgtgtgaccg gcattgttac caccttgagc
     2281 cctggccact acctgggcat tttctcccag ataatcaact attttacctt tcccttcgtc
     2341 accccagtga gttcccacaa ctatactaac tgccattttt ttctcctctt attcccattc
     2401 gatggtagcc ggaggcttgg aagtaatatc gtataccatt cttccaatcc ctgctacttc
     2461 attcactact cgcctcgcga tggcatccat ggtgtcatag gaaattcttg cccaatcagc
     2521 agtcattcca tcttcgctca tcaccgcccg taacactccc acatatttat aagttctttc
     2581 atctcctact actccaacac ttctcaccgg caccagtacc ccaaaagact gccacagctt
     2641 tttataaata ggagattttc ggagttcctg gttaattata aaatccatat cccgcaaaat
     2701 ctgcagacgg tcttcggtaa cctcgccaat tattctcacc gccagtcccg ggccaggaaa
     2761 aggctgcctc cagactattt cctcaggaat acccagctcc acagccaatt ttctaacttc
     2821 atctttaaaa agaaaacgaa gaggctctat caccttgagt tgcatatgct caggtaaccc
     2881 tcccacatta tgatgggttt ttatcctgac cgatggacca taaatggata tgctttctat
     2941 gacatcaggg taggtagttc cctgtaaaag ataagacaca tctcctaatt ttttcgcttc
     3001 ttcttcga
//
LOCUS       DAOPSS010000160         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_27093_length_2930_cov_9.090783, whole genome shotgun sequence.
ACCESSION   DAOPSS010000160 DAOPSS010000000
VERSION     DAOPSS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27093_length_2930_cov_9.090783"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKN31_07710"
     CDS             <1..540
                     /locus_tag="PKN31_07710"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873291.1"
                     /note="catalyzes the reversible phosphorylytic cleavage of
                     uridine and deoxyuridine to uracil and ribose- or
                     deoxyribose-1-phosphate; involved in the pyrimidine
                     salvage pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOA99842.1"
                     /translation="EELAQLGVRLFLRVGTCGAIQEGIAVGDLVISEGAVRMDGASPH
                     YAPLSYPACAHWEMVGALKKACELLRCRYHLGITCSSATFYPGQERYDTFSGYIISSL
                     RGSREEWKKLGVLNYEMEIATLFTVARVLGLRSGAICGVLVNRNEKEEVEEEKIEEIE
                     DRLSQVAAEAARLILSGEE"
     gene            543..1166
                     /locus_tag="PKN31_07715"
     CDS             543..1166
                     /locus_tag="PKN31_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99843.1"
                     /translation="MFLDYWLLEIEKIGEGWLIFLLFGGGIVSGFYFFRLRKVLFPLL
                     TFLVVMGGGGITRGIEKSNLETFSFSGDKASFFIFLFLALFLAGVVASKKGERWGRFF
                     SGFWLSANLYYLLIEISSSVELLRFLGHLSFTVVLFSSLLFALLSLNKKVLNFLSSFL
                     GSSLLVLAYLQVLYRWGGNDTFWDSSPWVLFLFLVMALWMMAVKESD"
     gene            1167..2057
                     /gene="hslO"
                     /locus_tag="PKN31_07720"
     CDS             1167..2057
                     /gene="hslO"
                     /locus_tag="PKN31_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOA99844.1"
                     /translation="MEKYEDKVVWSMVEDTTVVAYVASTTSLVEEARFLHNTSPVATA
                     ALGRALTLAGIMGAALKEGQKVTLHIRCLGPLESILAQANSQGEVRGYVSQPSVFVPS
                     REDKKLNVEEAVGKGSQLSVVKDLGLGEPYAGYIKMPRGGIAYDLAYYFALSEQLPSA
                     CSAGVYVGEKGEVLSSGGFIIHTPLGTPQHVIQKIEDNISLLPPVSSLILEGKSPEEI
                     TRDLLASLSYRVVGEKKLCYHCHCSRERARRTLILLGREELEELLAREGRAEVSCFFC
                     NRVYLFGDKELRELIEKVRK"
     gene            2070..>2930
                     /locus_tag="PKN31_07725"
     CDS             2070..>2930
                     /locus_tag="PKN31_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOA99845.1"
                     /translation="MKGILRIDYEEELNEEQKKVVFADEGPILVIAGAGSGKTRTITY
                     RVARLIEEGVDPSSLLLATFTNKAAREMLHRVECLLGISLEGMWGGTFHHIGNLILRR
                     EAKKIGYDNNYTILDREDQKDLLESTIEEMGIDRRSRRFPKGDVIANIISMSRNTFIS
                     VEDILEKFYPQFLSWASQLSSLFALYQDRKRQLSLMDYDDLLYYTWSLLQEDENTRRK
                     YGSAFHHILVDEYQDTNLLQAEIMDLLAQEHRNLLVVGDDAQSIYSFRGANFVNIMRF
                     PERYPDCQIFK"
BASE COUNT          740 a          515 c          772 g          903 t
ORIGIN      
        1 gaagagctgg ctcaattggg agtaaggctt tttttgcgag tgggaacctg tggagccatt
       61 caagaaggaa ttgcagtagg ggatttggtg atcagcgaag gagcggtgag gatggatggt
      121 gcttctcctc actatgctcc cctttcttat cctgcctgtg ctcactggga gatggtggga
      181 gctcttaaaa aagcttgtga acttttgagg tgtcgctatc acttagggat tacctgttct
      241 tcggctactt tttatcccgg gcaggaaagg tatgatactt ttagtggtta catcatttct
      301 tctcttcggg gtagcaggga agaatggaag aagttggggg ttttgaatta tgaaatggag
      361 atagccactc ttttcaccgt ggctcgagtt ctggggcttc gctccggagc gatatgcgga
      421 gtgctggtca acaggaatga aaaagaagaa gtagaagagg aaaaaataga ggagatagaa
      481 gaccgacttt ctcaggtggc agctgaagct gcccggctga ttctctccgg ggaggaataa
      541 acttgtttct tgattactgg cttttggaaa tagaaaaaat aggggaaggg tggttgattt
      601 ttcttctctt tggaggaggg attgtctctg gtttttattt ttttcgcttg agaaaagttc
      661 ttttccctct tttaaccttt ttagtggtga tgggaggagg gggaatcacc agagggatag
      721 aaaaaagtaa cttagaaact ttctcttttt caggtgataa ggcgagtttt ttcatttttc
      781 tctttctggc tcttttttta gccggagttg tagcgagcaa aaaaggagaa cgctggggga
      841 gattcttttc tggtttctgg ttgagcgcca acctctatta tcttttaata gagatctctt
      901 cttcggtaga gctgttgcgc tttttgggtc atctttcttt tacggtggtg cttttttctt
      961 ctctactttt tgcgcttctt tccctgaaca aaaaagttct aaatttctta agctcttttc
     1021 tggggagctc tcttctggtt cttgcttacc ttcaggtgtt gtatagatgg ggaggaaatg
     1081 ataccttttg ggatagcagt ccctgggtgt tatttttgtt tttagttatg gctctatgga
     1141 tgatggcagt taaggagagt gattgagtgg aaaagtacga agataaagtg gtatggtcta
     1201 tggtggaaga taccacagtg gtggcttatg ttgcctcaac cacttcttta gtagaagaag
     1261 caaggttttt acataatacc agtcctgtgg ctactgctgc cctgggaaga gcactaaccc
     1321 ttgccgggat aatgggtgct gctctcaaag aaggtcaaaa ggtaactttg catattcgtt
     1381 gcctggggcc tttagaaagc atattggcgc aggctaactc gcaaggagag gtgagaggat
     1441 atgtttctca accttcggtt tttgttcctt cccgggaaga taaaaaactt aatgtagaag
     1501 aagcagtggg gaaaggctct cagctttcgg tggtgaaaga tctgggatta ggggagcctt
     1561 atgctggtta tataaagatg cctcgaggag gcattgctta tgatttggct tattactttg
     1621 ccctttctga gcagcttcct tcggcctgca gtgccggtgt ttatgtggga gaaaaaggag
     1681 aggtgttatc atcaggtggg tttatcattc acactcctct gggcactcca caacatgtaa
     1741 tacagaaaat cgaggataat atttctctcc ttcctcctgt gagctctctt attctggagg
     1801 gaaaaagccc ggaggaaata actcgagacc tcttagcttc cctttcttat cgggtggtgg
     1861 gagaaaagaa actgtgttat cattgccatt gttctcgaga gagagcccgt cggactttga
     1921 tactacttgg aagagaagaa ttagaggagt tgttagcccg agaaggaaga gcagaagtta
     1981 gctgtttttt ctgtaatcgg gtttatcttt ttggagataa agagctcaga gagctgatag
     2041 aaaaagtaag gaaataggag gtttgattct tgaaaggaat tttgcgcatt gattatgaag
     2101 aagaattgaa tgaagagcag aaaaaagtgg tctttgccga tgaaggtccg attttagtga
     2161 ttgctggagc aggtagtgga aaaacccgga ccatcaccta tcgagtggca aggctgatag
     2221 aggaaggggt agatccttct tcccttcttt tggctacttt taccaataaa gcagcccgag
     2281 agatgttaca tcgggtggaa tgtcttttag ggataagcct ggagggtatg tggggaggga
     2341 ctttccacca tattggtaac ctcattctcc gccgggaagc aaaaaagata ggctatgata
     2401 acaactatac tattctggat cgggaagacc agaaagacct tctggaatct accatagagg
     2461 agatggggat agaccgccgt tctcgccgtt ttcccaaagg ggatgtgatt gccaacatca
     2521 tctctatgag tcgcaacact tttatctctg tggaggatat tttagaaaag ttttaccctc
     2581 agtttttgag ctgggcgagt cagttaagtt ccctttttgc tctttaccag gacaggaaac
     2641 gccagctctc tcttatggac tatgatgatt tgctttacta tacctggtct cttttacaag
     2701 aagatgaaaa tactcgccgc aaatatggaa gtgcttttca ccatatttta gtagatgagt
     2761 atcaggatac taaccttctg caggctgaga ttatggacct tttagctcag gaacaccgca
     2821 atcttttagt agtgggcgac gatgctcaga gtatttattc gtttcgggga gctaattttg
     2881 tcaatatcat gcgtttccct gagagatacc cggattgtca gatatttaaa
//
LOCUS       DAOPSS010000161         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_27456_length_2903_cov_7.481039, whole genome shotgun sequence.
ACCESSION   DAOPSS010000161 DAOPSS010000000
VERSION     DAOPSS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27456_length_2903_cov_7.481039"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKN31_07730"
     CDS             complement(<1..523)
                     /locus_tag="PKN31_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA99846.1"
                     /translation="MRKIVWSVVLAILLVGAAGVSFAQVLPEIPRAETLIVDILHGRI
                     GNPGNFNVWIPGSQAGHGLQQMLMDALWYVDPQTGEWINALAAEEPEYNEDFTKMTIK
                     IREGIYWSDGVPFTADDVVFTVKNIQSHPGMNSQPQLQEYVKDIYKTDDYTVEMELTK
                     SYSRMHNIFTSLVY"
     gene            complement(644..2026)
                     /locus_tag="PKN31_07735"
     CDS             complement(644..2026)
                     /locus_tag="PKN31_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99847.1"
                     /translation="MDKKAKVTVIGAGSAAFGLSTLVGILRHPDLQGVELFLHDIDEN
                     GLEKIRKLAEKMNEAWGSGVKINSSVERGKALEGADFVILSIAIDREKCWKLDRDIAL
                     RYGINHYAENGGPGAFAHTARNLSAIMPILQDMEKYCPEAWLLNFTNPVPRICTAANL
                     YSSIKTIGICHQINFGYFILAALFADELDLRLPRDFNFRWNDRSMSLFQVMSKKAREK
                     FIIRAWGLNHFTWMINVTEKGTGRDLYPEIERRMEAISPSFEPLTQEVFRVFGLLPVP
                     GDCHLCEYLPYTHNVSRKTWERYDIQMYDFEWAEKGRQKMWEKIERVVEGKEPLEGWK
                     EVETERGEFIIAGILKNLNSYEEAVNIPNQGYIVNLPDEVIVEVPAILGSEGLIGLGG
                     CELPEPIAELCRRQALINELVVRAIVEEDRKLAQEAFAIDPMIDDLEVAKKLLDDYLK
                     TFDSYLRFKL"
     gene            complement(2044..>2903)
                     /locus_tag="PKN31_07740"
     CDS             complement(2044..>2903)
                     /locus_tag="PKN31_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOA99848.1"
                     /translation="VEVHISPPKRDSSRLIAMVIDDIFNPFFTEVFAAAESTARTMGY
                     HLLLLPIEKDYDAFYLQDLKDKKVDGFILATRLSLSRLKEFFSAQVPLVFLGGVLDSP
                     FSRSILFPDEEGAYLATRHLVDLGHSRILCVGGSSKSSLFHNRFQGYKRALKEKQIPV
                     DPQLMVEITPTFEEARQLGLKYLSSENQISAVFAHNDPTACGIMAAAQELGMKIPEDL
                     SVVGFDNTFFSRLTCPPLTSVEIPKKLIGKRSVELLIRLMHNNGVESYVIEEVKLVER
                     KSTAFFSEK"
BASE COUNT          870 a          706 c          490 g          837 t
ORIGIN      
        1 cgtaaaccag agaagtaaat atgttatgca ttctagaata tgatttggta agttccatct
       61 ctactgtata atcatctgtt ttataaatat ctttcacgta ctcttgaagt tgaggttgag
      121 agttcattcc cggatggctt tgaatatttt taacagtaaa tacaacatca tcagcagtaa
      181 atggtacgcc atcgctccag taaatgcctt ccctgatttt tatggtcatt ttagtgaaat
      241 cttcgttata ttctggctct tctgcagcca aagcattaat ccattcacct gtctggggat
      301 ctacatacca taaagcatcc atcaacattt gttgaagccc atgaccggct tgagatcctg
      361 gaatccaaac attaaaattt cctgggtttc caatccgtcc atgtaagata tcaacgatga
      421 gagtctcggc tctgggaatc tccggtaaaa cttgcgcaaa gctcacacca gctgcaccaa
      481 ccagcaaaat agccagcacc accgaccaca ctattttcct catctttttc ctccttttca
      541 aaaatcaatc atgctatact caaaagtttc cttaccacca attaccacac gacttcccct
      601 taccagctct ttctatttta gccagatttc cccgcatcac ctcctataac ttaaaacgta
      661 aataagaatc aaaagttttt aagtaatcat ccagaagttt tttcgctact tcaaggtcat
      721 cgatcatggg gtctatggca aaagcctcct gggctaattt tcggtcttct tctacaatag
      781 ctctcaccac taattcattt ataagagcct gtctcctgca cagttcagca atgggttcag
      841 gaagttcaca tcctcctaat cctatcaggc cttcgctacc taaaattgcc ggaacttcca
      901 ctattacctc atctggcaga ttgactatat atccctggtt agggatattc accgcttctt
      961 cataagaatt gaggtttttt aatattccgg caataatgaa ttctcctctt tctgtttcta
     1021 cttccttcca tccttccagc ggctctttcc cttctactac tctctcgatt ttttcccaca
     1081 tcttttgtct tcctttttca gcccactcaa agtcgtacat ctggatatca tatctttccc
     1141 aggttttgcg actcacattg tgagtgtagg gaagatactc acagagatga caatctccgg
     1201 gaaccggtaa aagaccaaaa acccggaaaa cttcctgagt caaaggctcg aaagagggag
     1261 aaatagcttc cattcttctt tcaatttcgg gatagagatc cctccctgtt cctttttctg
     1321 ttacatttat catccaggta aaatgattaa gtccccaagc ccggatgatg aacttctctc
     1381 tggccttttt actcataact tgaaaaagag acatagatcg gtcattccac ctaaagttaa
     1441 agtctcgagg gaggcgcaaa tctaactcat cggcaaaaag agcagctagg ataaaataac
     1501 caaaattaat ctggtgacag ataccgatgg tctttatact ggaatacagg ttagccgcgg
     1561 tacagatcct gggaaccggg ttagtaaagt tcaaaagcca ggcctcagga caatattttt
     1621 ccatatcctg aagaatgggc ataatagccg ataaattgcg agctgtgtga gcaaaagcac
     1681 ccggccctcc attttcagca taatggttaa tgccatagcg aagagcaata tctcgatcta
     1741 acttccaaca tttttcccgg tcaatggcaa tggagagaat tacaaaatct gcaccttcca
     1801 gagcttttcc cctctctaca gagctgttaa ttttaacccc cgaaccccag gcctcattca
     1861 ttttctctgc caattttctg attttttcta aaccattttc atcaatgtca tgcaaaaaca
     1921 gttccactcc ctgcaaatcg gggtgacgca gtattcctac taaagttgac agaccaaaag
     1981 ctgcacttcc tgctccaatt accgtcacct tcgctttttt atccaacttt cacacctccc
     2041 ccattatttt tccgaaaaaa acgcagtaga cttcctctct accaacttca cctcttcaat
     2101 tacataggac tctactccat tgttatgcat tagacggatt aataactcca ctgaccgctt
     2161 accaattagc tttttaggaa tttctaccga ggttaaagga ggacaggtta atcgagaaaa
     2221 aaaagtatta tcaaaaccta ccactgataa atcctccgga attttcatac ccagttcttg
     2281 agcagcagcc atgatcccac aagcagtcgg gtcattatga gcaaagacag cgcttatttg
     2341 attttcagaa gaaagatatt ttaatcccaa ctggcgagct tcttcaaaag taggggtaat
     2401 ctctaccatt aattgcgggt ctacaggaat ttgtttttcc ttcaaagccc ttttataacc
     2461 ctgaaaacga ttatgaaaaa gagagctctt agaagagcca cctacacaga gaatccgaga
     2521 atggcctaaa tccaccaaat gacgagtagc aagataagct ccctcttcat cagggaaaag
     2581 aatactacga gaaaaagggc tatctaaaac tccccctaaa aaaaccagag gaacctgggc
     2641 agaaaaaaat tctttaaggc gagacaagct caggcgagta gccaatataa aaccatcaac
     2701 tttcttatct ttcaaatcct gaagataaaa agcatcataa tctttttcta taggtagaag
     2761 aagaagatga taacccatgg tgcgagcagt gctctctgct gcagcaaaaa cttcggtaaa
     2821 aaagggattg aagatatcgt ctataaccat tgcaatgagg cgtgatgagt ctcttttagg
     2881 gggagaaata tgcacctcca ccc
//
LOCUS       DAOPSS010000162         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_29455_length_2744_cov_6.182968, whole genome shotgun sequence.
ACCESSION   DAOPSS010000162 DAOPSS010000000
VERSION     DAOPSS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29455_length_2744_cov_6.182968"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..246)
                     /locus_tag="PKN31_07745"
     tRNA            complement(170..246)
                     /locus_tag="PKN31_07745"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:His,seq:gtg)
     gene            complement(329..1069)
                     /locus_tag="PKN31_07750"
     CDS             complement(329..1069)
                     /locus_tag="PKN31_07750"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOA99849.1"
                     /translation="MRFWEIDFLRGCAVITMVIYHLLYNLYSFGSLNINLYQGFWHYF
                     QVATASTFLALAGVSLTLSYNRIIQKGQKPSFIKYLKRGLRILGWGMVITVFTYFTLG
                     DWYVRFGVLHCIGVSTILGYFFLPLKSDVSTLIVSMLCLVLGSILSSQAFPFSFLLFL
                     GFKPYNFHTVDYFPLFPWWGVVLLGIFLGKQLYPGYKRIFSLPDWSRVPLIQAVSFLG
                     QHSLTIYILHQPIIIIILFLLGLIHLPI"
     gene            1441..2721
                     /locus_tag="PKN31_07755"
     CDS             1441..2721
                     /locus_tag="PKN31_07755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOA99850.1"
                     /translation="MSQILAIEGGEPINKKPFPTWPSFSEKTKQEALKPLENGLVNYW
                     TGSKGIEFEKKWAEYCGCRYGVSTTNGTSALHAALASCDIGPGDEVIVPSYSFIASSF
                     CVLQAGAIPVFADVREDTHTLDPQDVEQKITSRTRAIIPVHLYGIPCDMDAIMEIANR
                     HNLYVIEDAAQAHGSEYKGKKAGSIGHVGCFSFCQSKHFTTGGEGGCLVTNDEEIAWK
                     ARSFRDHGYDVKERLRLLEMEKKLFYIHERVGFNYRLTEIQSIIGLCELERFDSWNKP
                     RRIAIGERFLQAFQGHEGILHLPPHQKEDKLVSFWLFPIVVNMERLKCSSIKEFWQAV
                     EAEGVPVSPVLWPQMYRERAYLEHNGFGRVKFPFRSKEYTDPSAVEYDKVFCPNAAWL
                     EERTFCFPCHPVYDDEHVDLMIEAWEKVYEYYKR"
BASE COUNT          901 a          547 c          613 g          683 t
ORIGIN      
        1 agaggaacag ctctaagaaa ctttcccagc cctcaggttg cggaagtttt actacttgcc
       61 ccacagaaat cgcctctttc tgggagtaga tttatagcca tcggactcag gggtttttag
      121 agagaaaacg aggatgagac aagagcctta aatgcagtca atgcgcttct ggggtgagcg
      181 aggggattcg aacccccaac ccctggatcc acagtccagt gctctaaccg tttgaactac
      241 gctcaccact ttattcaaac ttaagtccat taatatttta cacttgttgc ctcttttaat
      301 caataccatt tgcccctaat gggacaagct atataggcag gtgaattagc cccagcaaga
      361 agagaataat aattataatg ggttgatgga gaatgtaaat agttagggaa tgttgaccca
      421 aaaaagacac tgcctgaatt aaaggcactc gagaccaatc gggaagggaa aaaattctct
      481 tatacccggg gtagagttgt tttcccagaa agattcccag tagcaccact ccccaccagg
      541 gaaaaagagg aaagtagtct acagtatgga agttataagg cttaaaacca agaaaaagaa
      601 gaaaggaaaa ggggaaagct tgcgaagaga gaatagaacc caaaaccagg cacaacatgc
      661 ttactatcag agtggaaaca tcgctcttta gaggaaggaa aaaatatccc agaatagtag
      721 ataccccaat gcaatgaagc actccaaagc gaacatacca atcacccaga gtgaaatagg
      781 tgaagacagt aatcaccatc ccccacccca gtattcggag acctcttttt agatatttaa
      841 taaaagatgg cttttgccct ttctggatga tacggttata gctcaaagtg agggaaactc
      901 ctgcaagggc aaggaaagta ctagcagtgg ccacctggaa gtaatgccag aatccttggt
      961 agagattaat gttcaaagac ccaaagctat aaagattgta aagaagatgg tatatgacca
     1021 tagttatcac ggcacaaccc cgcaggaaat ctatttccca gaaacgcata gctatttcct
     1081 ccacgtctta taatatcggg tattattagt tattcataga gaattataac agactataag
     1141 gagacaaatt cctcccctat agccccggaa aaacagaata aacgcaaaca aatatataaa
     1201 aactcaaaaa ggcaatctgc gacacgagtt taaaagcaca aatgatttaa aaactaggac
     1261 aacaatgccg aggaaaaata cccaacattg ttaagggaaa atgtatcgag attaaggtgg
     1321 acacccatgt caagacaaaa agaaaccttt attccccggg agcttcccta ttctgttata
     1381 attgagccga gaagggaagg agtttatccc caaagcctat tttaaaaagg aggatgaaaa
     1441 atgtctcaaa ttttagctat tgagggagga gaaccaatta acaagaagcc ttttcctacc
     1501 tggccatctt tttcggaaaa aacgaagcaa gaagcattga aacctctgga aaacgggttg
     1561 gttaattact ggacaggaag caagggaata gaatttgaaa aaaagtgggc ggaatattgt
     1621 ggttgccgtt atggagtttc taccactaat ggcacatctg ctttacacgc tgctttggcg
     1681 agctgtgata tcggaccggg agacgaggtg attgtaccct cctactcttt tattgcttct
     1741 tctttttgcg ttttgcaagc aggagctata ccagtttttg ccgatgtgag ggaagatact
     1801 catactttag atcctcagga tgtagagcaa aaaattactt ctcgtaccag agccatcatc
     1861 cctgtgcatc tttatggcat cccttgcgat atggatgcca ttatggaaat tgccaaccgt
     1921 cacaaccttt atgtgattga agatgctgct caagcccatg gtagcgaata taaaggcaaa
     1981 aaagcgggta gcataggtca tgtggggtgc ttcagcttct gtcaatccaa gcattttact
     2041 accggaggag aaggaggatg cctagtcact aatgatgagg aaatagcctg gaaggctcgt
     2101 tctttcagag accatggcta tgacgtgaaa gaacggttac gattgctgga aatggagaaa
     2161 aaactatttt atatccacga acgagtagga tttaattatc ggttgacaga aatccagtcg
     2221 attattggtc tttgcgaatt agaacgcttc gattcctgga ataaaccccg aagaatagcc
     2281 attggagaaa gatttttgca ggctttccag ggccacgaag gtattctgca tctcccccct
     2341 caccagaaag aagataaatt ggtttccttc tggcttttcc ccatagtagt aaatatggaa
     2401 agattaaaat gctcttctat aaaagaattc tggcaagcgg tagaggcaga gggagtgccg
     2461 gtttccccgg ttttatggcc tcagatgtac cgggaaagag cttatttaga acacaatggc
     2521 tttggaagag taaagtttcc cttccgctct aaagaatata ctgacccctc agcagtagaa
     2581 tacgataagg ttttttgtcc caatgctgcc tggttagagg aaagaacttt ctgctttccc
     2641 tgtcacccgg tatacgatga tgagcatgtg gatttgatga tagaagcctg ggaaaaagtt
     2701 tacgaatact ataaaaggtg aaaaatatgg ctgagagagt caag
//
LOCUS       DAOPSS010000163         2655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_30713_length_2655_cov_9.066154, whole genome shotgun sequence.
ACCESSION   DAOPSS010000163 DAOPSS010000000
VERSION     DAOPSS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30713_length_2655_cov_9.066154"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1873
                     /locus_tag="PKN31_07760"
     CDS             221..1873
                     /locus_tag="PKN31_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOA99851.1"
                     /translation="MIPFGRRYSRNFRRVREIIKVFRKYGFLYLFRRAHFPEHLTLPF
                     FRKEAARRPPEENLRLAFEELGTTFIKLGQILSTRLDLLPEEYCQELRKLQDRTSPVD
                     FLEIKEVVEQELKKPLEEVFEKFDPVPLASASIAQVHRAILKDGNKVAVKVQKPGVEE
                     KVISDLEILFYLANLADRTTSFAPLTFSEMVTEFQKSLMREIDFLYEAVNARRFRENF
                     SDFEGVYIPRVFDEYTTTRVLTLELIEGLNLSQMAEKGELEIDGPLLARRGAEAVLKM
                     IFEDGFFHADPHPGNIMIREETGELVFLDFGIVGTVDERTRENMVRMLLAILRKDIER
                     VVDILESEFLLSPVESPLALRVDLHEILERYVTANLKEMNISSLVNDFFYLLRKHNLR
                     FPPHLSSLMRALMVVEGTGMMLDPHFTVVPYLEKSLERVFAQRLRWERLSYKVSDYLF
                     DWRKLLVDFPDKAQGLLEDASRGRVRFQLESEDLRSLNRRLDRVSNRLSLSVILGSLL
                     IGSSLIYISSPRPGAMGILGILGFGIAAVLGIFLVIEMLRSR"
     gene            1913..2620
                     /locus_tag="PKN31_07765"
     CDS             1913..2620
                     /locus_tag="PKN31_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99852.1"
                     /translation="MKKLIIIFIALGLSLVLFNSFSWAHWVIEGEGKWQGEVYQFSWE
                     KEGGKMVLSLEEGEKSILRVGVDLAKPELSLIDDLSFTFSTLEDKNGIALSFLFALSS
                     ILGVKEEWLFAEKPSFFLLPDPLSLPPFGDCFPFRINDEATGWYFKEGIHPTMGEMIG
                     DLVEILEEGRLSLPFWREIVKVNGLIVAKEENEELVLRVYQVKEKDTPLELPQKGEDY
                     REVSWVDFLSPWEAILE"
BASE COUNT          740 a          466 c          624 g          825 t
ORIGIN      
        1 tatggagaag gctcttttgt ggttctgcga gcacaaaagt tagaaaacaa cgagttaaat
       61 tagtttcata caacctggca gaaacatttt ctcgaagcat gtgacagaga aacgtggaga
      121 gtaaaatgct tcaatgtttt tgagcttatt ttaaattaaa cacgaaaaag gcttgatatc
      181 tggtaaaata gtcgttagag cttttacagt gaggtaatag atgattcctt ttggccgtcg
      241 ttattccaga aattttcgtc gggtgcggga aattatcaag gttttccgta aatacgggtt
      301 tttatatctc tttcgccgcg ctcactttcc tgaacacctg accctgcctt ttttccgcaa
      361 agaagccgcc aggcgtcctc cagaagaaaa tttgcgttta gcctttgagg aattgggaac
      421 tacttttata aagttggggc aaattttaag tacccgtctg gaccttttac ctgaagaata
      481 ttgtcaggaa cttagaaagt tgcaagatcg gacttctccg gtagatttcc tcgaaattaa
      541 ggaagtggta gagcaagaac ttaaaaaacc tcttgaggaa gtttttgaga aatttgatcc
      601 tgtgcccctt gcctctgctt ccatagctca agtacatcga gcaattttaa aagatggaaa
      661 taaggtagca gtcaaggttc aaaaaccagg agtggaagaa aaagttattt ctgatctgga
      721 aatactcttt tatttagcca atttggccga tcgaacaact tcttttgctc cccttacttt
      781 ttcagaaatg gtgactgagt tccagaagag cttgatgagg gagatagatt ttttatatga
      841 ggcagtgaac gctcgccggt ttcgagaaaa tttttccgat ttcgaaggag tctacatccc
      901 ccgtgttttt gatgagtata ccactaccag agttttgact ctggagttga ttgagggcct
      961 taacctttct cagatggcag aaaagggtga acttgagata gatggccctc ttttagcccg
     1021 tcgaggagca gaagcggttc tcaaaatgat ttttgaagat ggttttttcc atgccgatcc
     1081 tcatcccggc aatataatga ttagagaaga aaccggagaa ttggttttct tagattttgg
     1141 aatagtagga acggtggatg agagaaccag agaaaacatg gtaagaatgc tgctcgctat
     1201 attgcggaaa gatattgaaa gagtagtgga tatattagaa agcgagtttt tgctctctcc
     1261 agttgaatct cctctcgctc tccgggtaga cctccatgag attttagagc ggtacgtaac
     1321 ggccaactta aaagaaatga atatttccag tctggttaac gattttttct atcttctccg
     1381 caaacacaat ttgcgctttc ctcctcacct ttcctccctg atgagagctc ttatggtggt
     1441 agaagggaca ggaatgatgc tcgaccctca ctttaccgta gtcccttatt tagaaaaaag
     1501 tctggaacgg gtttttgctc aacgtctccg atgggagaga ttgtcatata aagttagtga
     1561 ttatctcttc gattggcgaa aattactggt tgactttccc gataaagcac agggattatt
     1621 agaagatgca tcaagaggaa gagttcgttt ccagttggaa agtgaagatt taagaagctt
     1681 gaatcgtcgc ttagaccggg taagcaatcg cctttctctc tcggttattt tagggtcttt
     1741 actcattggt tcttccctga tttacattag ttctccccgg cctggggcta tgggtattct
     1801 ggggatttta ggttttggga tagctgctgt cttgggtata ttcttggtga tagagatgtt
     1861 gagaagtaga taaagggtgt aaatttttct caagaagaga ggaggaaagt tggtgaagaa
     1921 actgatcatt atttttattg ctcttggatt atccctggtg ctttttaatt ctttttcctg
     1981 ggctcactgg gtgatagaag gagaaggcaa gtggcaggga gaggtttatc agttttcctg
     2041 ggaaaaagaa ggagggaaaa tggtgctctc tctggaagaa ggagaaaaga gcatcctgcg
     2101 agtgggagta gatcttgcca aacctgaact ttccttgata gatgacttaa gctttacctt
     2161 ttctacttta gaggataaaa atggcattgc tctttccttt ctctttgcct tatccagtat
     2221 tttaggggta aaagaagaat ggctctttgc cgagaaacct tcttttttcc tccttcctga
     2281 tcctttatct cttcctcctt tcggagattg ttttcccttt cgcattaacg atgaagcaac
     2341 tggatggtat tttaaggaag gtatccatcc cactatggga gagatgatag gagatctggt
     2401 agaaatttta gaagaaggaa ggctttccct tcctttctgg agagagatag tgaaagttaa
     2461 cgggcttatt gtagcgaaag aggaaaacga ggagttagtt ttacgagtat atcaagtaaa
     2521 agaaaaggat actcctcttg agcttcctca aaaaggggag gactatcggg aagtttcctg
     2581 ggtagatttt ttatctccct gggaagcaat tttagaatag agggatgaaa tacgaaaaaa
     2641 atattgcttt ttatc
//
LOCUS       DAOPSS010000164         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_31077_length_2632_cov_6.849825, whole genome shotgun sequence.
ACCESSION   DAOPSS010000164 DAOPSS010000000
VERSION     DAOPSS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31077_length_2632_cov_6.849825"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..1259
                     /locus_tag="PKN31_07770"
     CDS             462..1259
                     /locus_tag="PKN31_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOA99853.1"
                     /translation="MDGKANLLPELEKLVEDSNLPLYLQLKNIIKAQIISGAIQPGDK
                     IPSESELCERYGVSRITVRQAINSLVQEGYLYRKQGVGTFVTSPKLRRRLPRLYSFSE
                     DMRELGLEPSSQVLEQRVIEADEEMAEILKLPDGSRKINKLVRVRLANGEPILLEKTF
                     IPYYLCPDLVKENLEEGSLYSTLREKYNLLLDHAYETYEVGKIRKEEAKILRCQVSLP
                     AFVIERLTYLKTEVPVEWTKSIARGDRLRFTVRLVADQAQIRREIEF"
     gene            1270..2256
                     /locus_tag="PKN31_07775"
     CDS             1270..2256
                     /locus_tag="PKN31_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOA99854.1"
                     /translation="MVKTAFLGIDLGGTNTKVALVDGEGTILERSIIPTRAMRKAEEV
                     VKDIASEAMKLRRRFEERGFRVQAAGIGIPGLIDWEKGICLLLPNFPNKWKEIPIREW
                     LEEELSLPVAVINDVRAITLAEKRFGVGKEVQSLVMMAIGTGIGGGVVLNGDLYIGKD
                     GSAGEFGHITVEPNGLRCGCGSRGCLEAYSSGPAMVAQALRALVQQNDTLIRDTVGDD
                     LSRVNPKVIAEAAQKGDGVAIEIIERAGSYIGQALSCICVVVNPEMIVIGGGVAQAGE
                     LLFKSIRQGLQERLFMIPVDTIQFVPAQLGMDAGAIGTATWAKERLEKGVIG"
     gene            2253..>2632
                     /locus_tag="PKN31_07780"
     CDS             2253..>2632
                     /locus_tag="PKN31_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA99855.1"
                     /translation="MSNTLKVGVIGTGFIGPAHIEASRRTFLAEVVSLADINEEVARE
                     KANQYAVKKYYGDYRDLLQDEEVEVVHICTPNYLHYQMSKDALLAGKHVVCEKPLAMN
                     RQEAEELVELADKKGLVNAVHFNIR"
BASE COUNT          761 a          422 c          689 g          760 t
ORIGIN      
        1 cagcctgacc ttctccctgg aaggacttcc ttcatctatc atgcctgaaa tttagcgagg
       61 taataatata gactcctcgt agaatcattc aggagtgacg ccccgccgtc attcccgaaa
      121 tcttttattg ggaatccaga ggtttctacc gaggggtgct ttctcctccc agtaactttt
      181 gttttttcgt tttggtcttt cggggattat aagggggaat tctttatcac ttatactgag
      241 ggagatcttt tttctccctc cgtggttttt gtttttttat gcttgttttt ttgggttttt
      301 atgtttttcc gggggttata agggaagtat cccccttata gttctcttct gttataatcc
      361 tttatgaggg tgatttgtag tttgtatcat gatggtttta gtggtttaat gttgttaaat
      421 gaggggcagg tgaaaagata atagatgcta aggaaaaagg aatggatgga aaggctaatc
      481 ttctgccgga attagaaaaa ttagtagagg atagtaatct tcctctttat cttcagttga
      541 agaatatcat taaagctcaa attattagcg gtgctattca accaggagat aaaattccct
      601 cagaaagcga attatgtgaa agatacgggg taagcagaat aacagtgaga caggctatta
      661 actccctggt gcaagaaggt tatctgtatc gcaagcaagg agtgggaact tttgttactt
      721 ctcctaagct acgacgaagg cttcctcgcc tttatagttt tagtgaggat atgcgagaat
      781 tagggttgga gcccagctct caagtgttag aacaaagggt gatagaagct gatgaagaaa
      841 tggctgagat tttgaaattg cccgatggga gtcgaaaaat caacaaattg gtcagagtaa
      901 ggttagctaa tggggagcct attttattag agaaaacttt tattccttat tacttgtgtc
      961 cagatctagt aaaagaaaac ttagaggaag gctcacttta ttctactttg agagaaaaat
     1021 ataaccttct gcttgatcat gcttatgaaa cctatgaggt gggaaagatt cgtaaagaag
     1081 aggctaaaat tttaagatgt caagttagcc tccccgcttt tgttattgaa aggttaactt
     1141 atttaaaaac tgaagttcct gtggaatgga ctaaatcgat agcgcgagga gatcgtctgc
     1201 ggtttacggt aagactggtg gctgatcaag ctcaaattcg tcgagaaata gaattttagg
     1261 aggtttcaca tggttaagac agctttttta gggatagatt taggagggac caataccaaa
     1321 gtagctttgg tagacgggga agggacaatt ttggagagga gcataatccc cacccgagct
     1381 atgcggaaag cagaagaagt ggtaaaagac attgcctcag aggctatgaa gctgcgaaga
     1441 aggtttgagg agcgaggttt cagggtgcag gcagcaggta tcgggattcc tggtcttatc
     1501 gactgggaaa aagggatttg tcttttgctc cctaattttc ccaataagtg gaaggaaatc
     1561 cccatccggg aatggttaga agaagaactg tctttaccgg tggcagtaat taatgatgtg
     1621 cgagctataa ctttagctga gaaaagattt ggagtgggaa aagaagtcca gagcctggtg
     1681 atgatggcta ttggtacggg aattggagga ggagtagtat tgaatggcga cctctatatc
     1741 ggtaaagacg ggtcagcagg agaatttggg catatcacag tagagccaaa tggactacgc
     1801 tgtggatgcg gtagcagagg atgtttagaa gcttattctt caggacctgc tatggtagca
     1861 caagctttac gagccctggt tcaacaaaat gatactttaa tccgggatac agtgggggat
     1921 gacctcagtc gagttaaccc taaagttatt gctgaagctg cccagaaagg ggatggagta
     1981 gccatagaaa taattgagcg agctggttct tacattggcc aggctctttc ctgcatttgt
     2041 gtggtagtta acccggagat gatagttatt ggaggaggag tggctcaggc aggagagctt
     2101 ctttttaaaa gcattcggca gggtttacaa gagaggcttt ttatgattcc ggtagatact
     2161 attcaatttg tgccagcgca attaggcatg gatgctggag ctattggcac agctacctgg
     2221 gcaaaagaga gattggaaaa gggggttata ggatgagtaa tactctaaaa gtaggagtaa
     2281 taggcactgg ttttattggt ccggctcata tcgaagcatc tcgtcggact tttttggccg
     2341 aggtggtaag tttagctgat ataaacgaag aagtggcccg agagaaggcc aatcagtacg
     2401 cagtaaagaa atactatggt gattatcggg atctcttgca agatgaggaa gtggaagtag
     2461 tacacatttg tacccctaat tatcttcatt accagatgag caaagatgca cttctggctg
     2521 gaaaacatgt agtttgtgaa aagcctttgg ctatgaatcg tcaagaagca gaagagttag
     2581 tagaactggc tgataaaaaa ggactggtaa atgctgttca tttcaatatt cg
//
LOCUS       DAOPSS010000165         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_31243_length_2620_cov_8.324366, whole genome shotgun sequence.
ACCESSION   DAOPSS010000165 DAOPSS010000000
VERSION     DAOPSS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31243_length_2620_cov_8.324366"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..872
                     /locus_tag="PKN31_07785"
     CDS             36..872
                     /locus_tag="PKN31_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOA99856.1"
                     /translation="MVLGVLILVSGFSSFSWGEKLSIGVSIAPLCSLVQEVGKDKVEI
                     FQLIPNGADPHTYEPRPSDVRKIEEGQAFFLVGLGLDFFLEEIIKNVAEGKPIFYLYQ
                     GINLIKEGEGANPHIWLSLPNAQIMVKNISQALSTLDPENSAYYERNAGEYIQKLAEL
                     DHWFREKVQNLRQRSFVAQHPAWDYLARDYNLELKGVIEKSPGKEPSPRELKNLIEEM
                     RKEDIRVIFAEPQLNQKIAQVLAQETNAQVVLLDPLGYFPEKPYFELMRENLNKILEA
                     MK"
     gene            875..1660
                     /locus_tag="PKN31_07790"
     CDS             875..1660
                     /locus_tag="PKN31_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOA99857.1"
                     /translation="MQEPIIEVKNVSFYYPPLEVLEDVNLTVKKGEFLAIIGPNGGGK
                     TTLLKIILGLLKPQKGEVYIAGKRVEELGKWRSKIGYVPQTAQIDYTFPITVEEFVMT
                     GRFGLIGLGKRPQAHDWEMVRKALREVKMEEWSKKQIGELSGGQRQKILIARSLALEP
                     EILILDEPTTAVDPESSESFYELLLRLHEQGITIITVSHDVGVVAQYVDSIACLNRRL
                     IKHDRPEEVIREGVLEEMYGREAAFFHHGAIPHIVVRETPPER"
     gene            1689..2471
                     /locus_tag="PKN31_07795"
     CDS             1689..2471
                     /locus_tag="PKN31_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOA99858.1"
                     /translation="MQRALFIGLIVGVLGATLGVFVVLKRLSFMGAGISHAAFGGIAL
                     GYFLGWDPILTAVIFCISVGIGISMLIENNRIKEDTAVGIFFTSTMALGILLIGLKEG
                     YNIDLFGYLFGNILAVGREDLVISLAIGAIIAFSLYFFFKELILSILDPEMARVIGLP
                     VSFLNHLLVALIALFIVISVRIVGIILVSALLTIPAATALQWSKNLRKVLVLSSIDSL
                     GCVLGGLALSYYLDTSPGATIVILATLVFFTTFLLPLPLKKK"
BASE COUNT          773 a          484 c          609 g          754 t
ORIGIN      
        1 attgggaggt aaaaattttg aaaaaatgcc ttcgagtggt tctgggggtt ttgattttgg
       61 tttctggttt ttcttctttt tcctggggag aaaagctatc aataggagta tccattgctc
      121 ccctgtgtag tttggttcag gaagtgggaa aagataaagt agaaatattc cagcttatac
      181 ctaacggagc ggatccccac acctatgagc ctcgtccttc cgatgttagg aagatagaag
      241 aaggacaggc tttttttctc gtgggattgg gcttggattt ctttctggag gaaataatta
      301 aaaatgtagc agaggggaaa cccatctttt acctgtacca ggggataaat ctcataaaag
      361 aaggagaagg agccaacccc catatttggc tttctcttcc caatgctcaa ataatggtaa
      421 agaatataag ccaggctcta tctaccttag acccggaaaa cagtgcctat tatgagagaa
      481 acgccgggga gtacattcaa aaactcgcag agctggacca ctggtttaga gaaaaagttc
      541 aaaatttgcg tcagcgctct tttgttgccc agcatcctgc ctgggactat ttagctcgag
      601 actataacct ggaactaaaa ggagtgatag aaaagtctcc cggaaaagag ccctctccta
      661 gagagctcaa aaatctgata gaagaaatga gaaaagaaga tattcgagtg atttttgccg
      721 aacctcagct caatcaaaag atagcccagg tattagctca agagaccaac gctcaagttg
      781 tccttcttga ccctttagga tattttccag aaaagcccta ttttgagctt atgagagaaa
      841 acttaaataa aatcctggag gctatgaaat aaagatgcaa gagccaatta tagaagtaaa
      901 aaatgtctct ttttattatc cccctcttga ggtattggaa gatgtaaacc tcacggttaa
      961 aaagggggag tttctggcta taatcggacc taatggggga gggaaaacca ctttactcaa
     1021 aataattctg ggacttttga agcctcaaaa aggagaagtg tatatcgcgg gaaaaagagt
     1081 agaagagctg gggaagtgga gaagtaaaat tggctatgtt cctcaaactg cccagattga
     1141 ctacactttt cccattactg tagaagagtt tgtgatgacc ggaagatttg gcctgatagg
     1201 cctagggaaa agaccgcagg cacatgattg ggaaatggta agaaaagcct tgagagaagt
     1261 aaaaatggaa gaatggagca aaaaacaaat tggagaacta tctggaggac agagacaaaa
     1321 aattttgatt gctcgctcac tggctcttga gcccgagata ttgattttag atgaacccac
     1381 aacagcagta gatccagaaa gtagtgaaag tttttatgag ctccttcttc ggctccatga
     1441 gcaaggaatt accatcatca ctgtttctca tgatgtggga gtggtagctc aatatgtcga
     1501 tagtattgcc tgcctaaacc gaaggttgat aaaacatgac cgcccggaag aggtaataag
     1561 agaaggagtg ctggaagaaa tgtatggcag ggaagcagca ttctttcacc atggtgctat
     1621 cccccacatt gtagtgcggg aaacacctcc cgagaggtga agagttggaa atcttacgat
     1681 ttactttcat gcaaagagct ttatttatcg gactcattgt gggggtttta ggagcaacgc
     1741 tgggggtttt tgtagtttta aagaggcttt cttttatggg ggcagggata tcccatgctg
     1801 ctttcggggg tatagcttta ggatattttt tagggtggga ccctatttta accgcggtaa
     1861 ttttttgtat ctcggtaggg ataggaatat caatgctcat cgaaaacaac cggataaagg
     1921 aagacactgc agtaggtatt ttttttacca gcactatggc tttaggaata ctactcatcg
     1981 ggctaaaaga agggtataac atcgaccttt tcgggtattt atttggaaat atactggcag
     2041 taggtagaga ggacttagta atatctctgg caataggagc aataattgcc ttttctctct
     2101 acttcttttt taaagaactg attctctcca tattagatcc ggaaatggcc cgggtaatcg
     2161 ggctaccggt cagcttctta aaccatcttc tcgttgcttt aattgctctt tttattgtca
     2221 tttctgtaag aatagtggga attatactgg tatcagcact tttaactatt cctgccgcca
     2281 cagctttaca gtggagcaaa aacctcagaa aggttttggt cctttcctct atcgatagct
     2341 taggatgtgt cttgggagga cttgccttat cctattattt ggatacttct cctggggcaa
     2401 ccatcgtaat attggccact ctggtatttt ttacgacttt tctcttgccc ttacctttaa
     2461 agaagaagta actttatttt ttctttttca acctccgggt ttatactcaa ggcctcggcg
     2521 ctcttttgac ccctttcacg atgatgtgtt ttccctaaag ctgtgtttgg tagaatagaa
     2581 atgcagcaca gcggaagtta aaaagtacag caggaaatag
//
LOCUS       DAOPSS010000166         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_32149_length_2559_cov_6.382987, whole genome shotgun sequence.
ACCESSION   DAOPSS010000166 DAOPSS010000000
VERSION     DAOPSS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32149_length_2559_cov_6.382987"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..535
                     /locus_tag="PKN31_07800"
     CDS             401..535
                     /locus_tag="PKN31_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99859.1"
                     /translation="METKKFVYWQEGNIWVGYLEEHPDYWTQGESVEKLKENLRELYQ
                     "
     gene            complement(765..1151)
                     /locus_tag="PKN31_07805"
     CDS             complement(765..1151)
                     /locus_tag="PKN31_07805"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HOA99860.1"
                     /translation="MAILQVYQRQLEVAMQKFGDIVSEGEIIYTESKEPLKLRLNIVD
                     GSIVDVFYSSGGKYSYHWDRRIINGTIYRHDNAPHKRWREIKTFPKHFHEETEYAAKE
                     SYISADPLFAIEEFLEFVKSMLIGSV"
     gene            complement(1117..1410)
                     /locus_tag="PKN31_07810"
     CDS             complement(1117..1410)
                     /locus_tag="PKN31_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99861.1"
                     /translation="MKAEIIEEISRYIALDKNSLLDKGVESLLKEKKRGVMLDRLEIL
                     SRYKVSSAEELERKIREGEIEEHPAWEDLIALENLDSALEKINGYLASLSAST"
     gene            complement(1504..1992)
                     /locus_tag="PKN31_07815"
     CDS             complement(1504..1992)
                     /locus_tag="PKN31_07815"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HOA99862.1"
                     /translation="MPVVSDASPLILLAKIRRLDLLKKLYSEVLIPKEVKEEVTKQEV
                     PPIISGIREGWIKVKEVELSSEVKEMGRELGLHEGEIYALSLALHANVKQVLADDKLA
                     RVGARILGLQAIGCLGVVMKAYEMDVITRNEAISAIQELVKAGLWISPEVLGEVLTSL
                     ED"
     gene            complement(1976..2281)
                     /locus_tag="PKN31_07820"
     CDS             complement(1976..2281)
                     /locus_tag="PKN31_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HOA99863.1"
                     /translation="MSGEVMAQLPEELMKEIEYWAEREKVDKSTLLTKIMEEGLNVWK
                     MNKALEMYRLQKVTLWKAAEIAGVSLAEMLAELPKRKIIFQYDLDELREDLEYARSK"
BASE COUNT          649 a          608 c          476 g          826 t
ORIGIN      
        1 agtttttgct ttttctttgg ttttaaagac tgtggactgt ggaccccggc ttaaagattg
       61 ccggggtgac gaacgaaggg gtagtagaag cattcaaggg caggctaaaa acggctcgaa
      121 agttgccacg gggtgacgaa taaggagggt cgttgcagtg acagaaaata agggattgat
      181 aagagggaat tctttcccct ttatgctgag aaagacgttt ttcctccctc agtggttttt
      241 acgtttttct tttaattcct taagggggat cagtttcctt ctatagccgg ggccatagga
      301 ggttcactcc ttgtagctat agttcggtca ctgaagcttc caagagatga aaggtattcg
      361 cagactttaa aaggtacctg gctgagagga ggtagaccac atggaaacga aaaagtttgt
      421 ctactggcag gaaggcaaca tatgggttgg atatcttgag gaacaccccg attactggac
      481 ccagggggaa agtgtggaga aattaaagga aaacttaaga gagctttatc agtaattaag
      541 cagtggagca attccttgtg ttcgcaaaat ggctgacttt ggaagtggga tggaaagaag
      601 agacctgctt tgaaaattag aagaaatggg ttgtgttctt atcagacatg gtaaagagca
      661 ttatattggt tcaaaatccg cggacgaagg cctcacaacc aatttcccac tatctactgt
      721 aacaaagccc ggagttggga gattctgcct cggcctcctt cttgctacac acttcctatc
      781 agcatgcttt ttacaaactc taaaaattcc tcgatggcaa acaatgggtc agcacttatg
      841 tagctctcct tagcagcgta ttccgtctct tcatgaaagt gtttcgggaa agtcttgatt
      901 tccctccagc gcttatgtgg agcattatca tgccggtaaa tcgtcccatt aattattctt
      961 ctatcccagt gataggagta tttccctcca gatgagtaaa aaacgtctac aattgagcca
     1021 tccactatat ttagccttaa ctttaatggc tctttgcttt ctgtataaat gatttcgcct
     1081 tcactcacga tgtcaccaaa tttttgcatt gctacttcaa gttgacgctg ataaacttgc
     1141 aagatagcca tttatttttt ccaatgcgga atcaagattt tccagagcta ttaagtcttc
     1201 ccaggctgga tgctcctcta tttctccctc ccgaattttt ctttctaatt cctctgctga
     1261 agaaacttta taccgagaga gtatttccag tcggtcaagc atgactcccc ttttcttttc
     1321 ctttaaaagt gattcgacac ctttgtccag caaggagttt ttgtccagag ctatataccg
     1381 acttatctcc tcgattatct cagccttcat tgctatcctc ctcgtgaaat cttgcatcca
     1441 atgctattat atcatgacca tctataatcc aatgtacgat gattgtccct tttcatatcc
     1501 ccgtcaatcc tccaacgaag tcaacacctc tcccaagacc tcaggggaaa tccataatcc
     1561 tgctttaacc aattcctgaa tagcactaat tgcttcgttc ctggtaatga cgtccatttc
     1621 atatgctttc ataacaacac ccaggcatcc gatagcctgt agccccaaaa ttctagcacc
     1681 aactcttgcc aatttatcat ccgctaaaac ctgctttaca tttgcatgca aagcgaggga
     1741 cagtgcatat atctctccct catgtagtcc cagctctctt cccatttcct ttacttccga
     1801 agaaagctct acctctttca cttttatcca tccttctcta attcctgaaa ttattggagg
     1861 aacttcttgc tttgttactt cctctttaac ctcttttggg atgagcacct ctgaatataa
     1921 tttctttaaa aggtctaatc ttctgatttt ggccaggaga atgagtggac ttgcatcact
     1981 tactacgggc atattcgagg tcctctctca actcgtcgag gtcatactga aatataatct
     2041 ttctctttgg caattctgca agcatctcag ccagcgaaac acctgctatt tctgcggctt
     2101 tccataaagt cactttctgt aacctgtaca tctccagtgc tttgttcatc ttccatacat
     2161 ttaatccttc ctccattatc ttggttagca atgtggattt atctactttt tccctttctg
     2221 cccagtactc tatctctttc ataagctctt caggaagctg cgccataact tctccagaca
     2281 tatttattca ccccctgttt tggtaattca accgaaacaa tgttcgaggt tctgtattga
     2341 ttcttagaac ctggcattct tctatatttt atcactaatc cagccgtata ttttttgata
     2401 ccattcttca ttttcgtcct gggctcatag tctaaagccc tgcttgtctt ctcgatggag
     2461 tctccaccac gcgtgatttg cctatcttat tatcactccg aactggagcc ggagagcttc
     2521 ctcttctgtt atgcctgaaa tcttttatcg ggaatccac
//
LOCUS       DAOPSS010000167         2542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_32417_length_2542_cov_5.827101, whole genome shotgun sequence.
ACCESSION   DAOPSS010000167 DAOPSS010000000
VERSION     DAOPSS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32417_length_2542_cov_5.827101"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..399)
                     /locus_tag="PKN31_07825"
     CDS             complement(232..399)
                     /locus_tag="PKN31_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99864.1"
                     /translation="MNKGTLSPGGEEEVFREMCDNVLLLREPVTNFSQVLKDLAKYFS
                     EWVVLFKDDTI"
     gene            complement(457..2388)
                     /locus_tag="PKN31_07830"
     CDS             complement(457..2388)
                     /locus_tag="PKN31_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOA99865.1"
                     /translation="MLGVCYYPEHWSKEQITSDIPRMRELGIQWVRIGEFAWSLIEPA
                     PGKFEWQWLDEVLNLFELEELKVILGTPTATPPKWLVDRCPDILPVDKEGRIRKFGSR
                     RHYCFSNSNYREEVRRIVSILAQRYGNHRAVSGWQLDNEYGCHDTVRCYCPSCQKAFR
                     MWLENRYGDIDTLNEAWGTSFWSQRYRSFEEIELPNLTVTTPNPSHLMDYFRFSSNQV
                     CSFNQLQVEILRKLSPHRFITHNFMGGFVDFDHFTLAQDLDLASWDSYPLGHTISFFG
                     STDLEESYIRRGHPDISAFSNDLYREVGGGRFWIMEQQAGPVNWAPYNPIPAPGMVRL
                     WTLEAIAHGAEVVSYFRWRQVPFAQEQMHSGLLHPDSTPGPGFYEVQKVARELANIEL
                     PSVRKSSVALIFDYEAAWVFEIQPHGEGVSYLHLVFQFYSALRQLGLDVDVLSPRASL
                     EGYKFVVVPSLPIVSKEALESLQDFAGIVVFGPRTGSKTETYSIPLVLPPGPLQELIS
                     LKVTQVESLPPQMEKVHWQNREYPVGIWKEWVETELQPEMTFADGRGAAFRYDRYYYF
                     AFWPGEALLVDFFAWLVKEAGLNPERLPKGLRLRRRGDLVFAFNFADQVSEVPAPATA
                     HFLLGSREIQPGEVAIWRE"
BASE COUNT          726 a          644 c          497 g          675 t
ORIGIN      
        1 cccaataaca catatttgcg agctaatttc atttattcca acctccttcc tgattatgaa
       61 ttagctaact caacaactca gctcatccga gctaacaact atagaatatc tcctatcgct
      121 cagattgtca aacagaggag aagcaaaaat aatgccaacc ccttgtaggg atatttttat
      181 tttcccctct attattagct tttgaaactc tctccattca gcattagata attagatagt
      241 atcatcttta aataaaacaa cccattcaga aaagtattta gctaaatctt ttagaacttg
      301 agagaaattg gtgaccggtt ctcttaaaag aaggacattg tcacacatct ctcgaaagac
      361 ttcctcttct ccccctggtg atagtgttcc tttattcata gagtaaaagc catctgttac
      421 acctcaccgc tttcttatcc tggagatata aagattttat tcgcgccaga tggctacctc
      481 accaggctgg atttccctgc ttcccagcaa aaaatgggct gttgccggag caggaacctc
      541 agaaacttgg tcagcaaagt taaaggcaaa aaccaggtct cctcttcttc ggaggcgtaa
      601 ccccttaggt aatcgctccg gattgagacc ggcttctttt actaaccagg caaaaaagtc
      661 caccaaaagt gcctcaccgg gccagaaggc aaaatagtaa tatctgtcat accggaatgc
      721 cgctcctctg ccgtcggcga aggtcatctc tggctggagc tctgtctcaa cccattcttt
      781 ccagataccc accgggtatt cccggttctg ccagtgtact ttctccatct gaggcggtaa
      841 actttccacc tgggtaactt ttaaggagat aagctcctgc aaaggtccgg gaggaagaac
      901 gagaggaata ctataggttt ctgttttgct tcccgttcgc gggccaaaaa ccactattcc
      961 cgcaaaatcc tgcaaactct ctaatgcttc tttgctcact atgggtaagc tgggcactac
     1021 cacaaattta taaccctcaa gagaggctcg aggagaaagc acatcgacat ccaaaccaag
     1081 ttgtcgtagg gcactgtaaa actggaaaac cagatgaaga tagctgactc cctcaccatg
     1141 gggctgaatt tcaaaaaccc aggcagcctc gtagtcaaaa atcaatgcca ccgagctttt
     1201 cctcacagaa ggaagctcaa tatttgctaa ctctcgagct actttttgta cttcgtaaaa
     1261 gccagggccg ggggtagaat caggatgcaa caaacctgaa tgcatctgtt cctgagcaaa
     1321 aggtacctgc ctccagcgga aataagaaac cacctctgca ccatgagcta tggcttccag
     1381 tgtccagagc ctgaccattc caggagcagg aatagggtta taaggagccc agtttaccgg
     1441 tcctgcctgc tgttccatta tccaaaatcg ccctcctccc acttcccgat agaggtcatt
     1501 actaaaagca gaaatgtcgg ggtgacccct gcgtatatag ctttcctcta aatcggtaga
     1561 gccgaaaaac gatatagtgt gtcctaaagg gtaactatcc cagctggcta aatcaaggtc
     1621 ttgagccaga gtgaaatggt caaaatcgac aaatcccccc ataaagttgt gggtgataaa
     1681 gcggtgaggg gaaagcttcc gcaggatttc cacctggagc tggttgaaag aacatacctg
     1741 atttgaggaa aaccggaaat aatccataag atgagagggg ttaggagtgg tcaccgttaa
     1801 gttaggaagc tcgatttctt caaaagaacg gtatctctga ctccagaaac ttgttcccca
     1861 ggcttcgttg agagtatcta tatccccgta gcgattttca agccacatcc tgaaggcttt
     1921 ctggcatgaa ggacagtaac accgtactgt atcatggcat ccatattcat tatcaagctg
     1981 ccagcctgaa actgccctgt gattaccata gcgctgagcc aatatcgaaa caatacggcg
     2041 gacttcttcc cggtaatttg agttagaaaa acagtagtgt cttcttgaac caaactttcg
     2101 tattcgccct tctttatcca ccggaagtat atcggggcaa cgatcaacca accacttagg
     2161 aggggtggca gtgggcgtgc ccaatatcac tttgagctct tcgagctcga ataaattcag
     2221 cacttcatcc agccattgcc attcaaattt ccccggagct ggttcaatta aactccaggc
     2281 aaactccccg attctcaccc actgaatacc cagctcccgc attctgggaa tatcacttgt
     2341 tatttgttct ttactccagt gttccggata gtaacaaact cccaacatct ttttattcct
     2401 ccttattaga caggttttat aacttacatt gcagataaat cttactttac tttcccacta
     2461 tactgatgac attttaccaa aacaccatcg actaaaactt cccgaggtat ttctttttta
     2521 catttttcca taaccacagg ac
//
LOCUS       DAOPSS010000168         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_33813_length_2465_cov_8.314938, whole genome shotgun sequence.
ACCESSION   DAOPSS010000168 DAOPSS010000000
VERSION     DAOPSS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33813_length_2465_cov_8.314938"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1894)
                     /gene="glpQ"
                     /locus_tag="PKN31_07835"
     CDS             complement(32..1894)
                     /gene="glpQ"
                     /locus_tag="PKN31_07835"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: protein motif:HMM:NF008354.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOA99866.1"
                     /translation="MFSDVQDKFPINPQTIEAMNNILDFEKPEFVILVGDNCTGAIGS
                     AEEFNLYVQQMVEPMEKRGIPWAHVYGNHDEDHVEKTGLTKELQQGIYESFPHCLSQR
                     GPEEVEGVGNFVLPVYSSDGSKEVFAIWGLDSNAYLEDNNSWYGGRLENDAILENGLQ
                     GSHNYDFIKFSQIRWYWDTSVEMEKKWGYKIPGILFTHIPLPEHRLIVMNPEPTGIIG
                     EKNEDVCNSPINSGLFAAILQRGDIKGIFVGHDHVNDFVGNYCGIKLGYDANIGFGTY
                     GLEGEDKDRLRGARVFRINERTPQAIETWMRYASEFDKNKSSELLSSLLVIAHRGASG
                     YLPEHTLESYAMAYALGADYIEQDVVLTKDKVFICLHDIYLEPTTNVEELYPERKRED
                     GHWYAADFTLEEVKKLRVHERSKADGTPYFPERFPVNYSMFEVPTFEEAIQLIQGLNK
                     STGKNIGIYPEFKQPSWHEKEKLEGGEKILLDILQKYGYTEKNSGIFVQCFEPEALQK
                     MRFELGTELPLIQLIGSGEEKDAQMLTSEGLEEIASYADGIGILKDYIENNPELVSLM
                     REKGLLVHAWTFRKDAVPEKYPSYEKELEQLCRECNIDGLFTDFPDIVIKFLER"
     gene            2125..2271
                     /locus_tag="PKN31_07840"
     CDS             2125..2271
                     /locus_tag="PKN31_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99867.1"
                     /translation="MKKERFCVILPSFLCQSFGHLWGIEVEIWLKCAILRYEEIIRGV
                     DLAY"
BASE COUNT          685 a          538 c          453 g          789 t
ORIGIN      
        1 tctctttttc tgaagagagc agcaaagaat cttatctttc taaaaacttg attactatat
       61 ccgggaaatc tgtaaacaac ccatctatat tgcattcccg gcaaagctgc tccagctcct
      121 tttcataaga aggatatttt tctggaacag catcttttct gaaagtccag gcatgaacca
      181 atagcccttt ttcccgcatc aaactaacca gttccgggtt gttttctata taatctttta
      241 agattcctat gccatcggca tagctggcta tttcttctaa gccttcgctg gttagcatct
      301 gggcatcttt ttcttctccg ctaccgatta actggattag aggaagctca gttcccagct
      361 caaagcgcat tttttgaaga gcttctggtt caaaacactg gacaaaaatt ccggaatttt
      421 tttctgtata cccatatttc tgaagtatat cgagaaggat tttttctccc ccctcgagtt
      481 tttctttttc gtgccagctg ggttgcttga attcgggata aattcctata tttttacccg
      541 tacttttgtt aagcccttga atcaattgaa ttgcttcttc aaaggtaggc acttcaaaca
      601 tagaataatt taccggaaag cgctcaggaa aatagggtgt gccatcggct ttcgaacgtt
      661 cgtgtactcg tagtttttta acctcttcca gagtgaaatc agcagcatac cagtgtccat
      721 cttctctttt gcgctctgga tataattcct caacgttggt agtaggttca agataaatat
      781 cgtgcaagca aataaagact ttgtctttgg tcaggactac gtcttgctct atgtagtctg
      841 ctcctaaagc ataagccata gcataagatt ctaaggtatg ttctgggaga tatccactgg
      901 ctccccggtg agcgattact aaaagggaag aaagcaattc tgagctctta tttttatcaa
      961 attcactggc atagcgcatc caggtttcta tggcttgagg agttctttcg ttaattctga
     1021 acactctggc acctctcagt cggtctttgt cttctccttc taaaccatag gttccgaatc
     1081 ctatgttggc atcatagccc agtttgattc cgcagtaatt gcccacaaaa tcattaacgt
     1141 ggtcatgacc tacaaaaata cctttgatat ctcctctctg taagatggca gcaaatagtc
     1201 cactgtttat gggagagtta caaacatcct cgtttttctc tcctattata ccagttggtt
     1261 ctggattcat aactatcagt ctatgctcgg gtagaggtat atgagtaaag agaatgcccg
     1321 ggattttata gccccatttt ttctccattt ctaccgaagt gtcccaatac catctaatct
     1381 gggagaactt tatgaaatcg tagttatggc taccctgtaa tccattctct aaaattgcat
     1441 cattttcaag cctgccacca taccaggaat tattatcttc aaggtaagca ttggaatcta
     1501 acccccaaat tgcaaacact tctttactgc catctgaaga atataccggt aaaacaaagt
     1561 tacctactcc ttcaacttct tcaggaccac gctggctcaa acagtgagga aatgattcgt
     1621 aaattccttg ttgtaactcc ttggtaaggc ctgttttttc tacatggtcc tcgtcatgat
     1681 taccataaac atgagcccag ggaattcctc ttttttccat aggttctacc atttgctgta
     1741 cataaagatt gaattcctct gcgcttccga tagctccagt acaattatcc cccaccagta
     1801 tcacaaattc cggtttttcg aagtctaaaa tattgttcat agcttctatg gtctggggat
     1861 taatgggaaa tttgtcctgc acatctgaaa acataacaat cttaaaggta ccctgagaat
     1921 taaatttaag cgtgggcttt gggggaagaa cagcttgtgc ctccaatgag cacattgata
     1981 acaatgagcc cacgcatatt ccaaagaatg ccaagattaa aagacttctt ttcataaaac
     2041 tacctccttt tcattttctc cagtttaaga gaagtttttt aagagcaaat taagctaatg
     2101 ttaaatttat ttaaaaacgc ggtaatgaaa aaagagagat tttgcgtcat tttgccatct
     2161 ttcctgtgcc agagctttgg ccatctctgg ggaatagagg tggaaatctg gctcaaatgt
     2221 gctatattac gatatgagga aataataagg ggggttgatt tagcctacta agagctctga
     2281 gccgggaaag tcccttttct actgcggtta ttcaatccgg aacttaattt attatgttat
     2341 agccctcata gcacactaca ccatcatcga agaaaaaagc tcgagacctg gtggtttttt
     2401 ataaaaaatc agatgacccc agaaatataa gcctttctac ctgggctttg acaacgtgag
     2461 tggag
//
LOCUS       DAOPSS010000169         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_35193_length_2390_cov_6.504497, whole genome shotgun sequence.
ACCESSION   DAOPSS010000169 DAOPSS010000000
VERSION     DAOPSS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35193_length_2390_cov_6.504497"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            528..2066
                     /locus_tag="PKN31_07845"
     CDS             528..2066
                     /locus_tag="PKN31_07845"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOA99868.1"
                     /translation="MNKNRFLQGRGKLVILVLLALLLGGSTLAGAQPRISIRFQLLPD
                     VYISIPRSPQITLSIDKGEGANYYIDDPITIRYRANRDGYVNLIDYLPDGGVSILVRD
                     EFIRSGEEGRFAGKVYGPAGVERIVVLLTPDKVSERDLESFIKSPHQADRIFGTRYAT
                     NRIHFQVLARMESTVLTIEPDDLTMDPGTSVVLTATLKDVRGRPITGEEIRWSANAGR
                     LSTSRTTTDLRGQGRVTFYAPSQPDTVTIEARFPGGVNSASASARATIRVERKTYYTT
                     LEVAPSSFTIDGGGNIKLVATLKDENGKPVYGRTIYWEVTEGDLDKYSTPTDSSGKAV
                     VYYYAPEVDEVTDVKITAQFRGVGGLAPASFEVWGTIEPLPVALTSSIFYLDFGSGTP
                     RGNIDRLRYSGDIVLGFSLNDLYVLEMKPREAIEFEFNPGGSPREGAIFLWLQGESGG
                     KARVSVNGNRLATISVSRDLIAPGGEEKIIIPRNVLVRGINRLRIEADPGRNIRVQKV
                     IIVF"
BASE COUNT          608 a          517 c          577 g          688 t
ORIGIN      
        1 gaatttatta cctcaaactg cttgactttt aacatagaac attcccgaaa tctttaatcg
       61 ggaatccacg tttttgcttc ttcttttgtc cttccattgg gaatcatagt ggcgttttct
      121 cctattattt ggattcctta taaatacact caggggtgac aaaaaagagg agaggtttat
      181 aagggggaat tctttcccac ttatactggg aaatcttttc ttcccggtag ttttttgcat
      241 tgtctgtttt ctctccctca gtaggttttt tgttttttca agggatcata ggggatatta
      301 gttcccctat aatctgccgc gggctacccg ctccgagggt aaaaaagagt tgcttcctca
      361 gccgcttctt gcaaatacaa aaggagagga attatagcag attttgcggg tgctattggc
      421 cactaaagca gcctgttccc cccctctgtc gggaaaggaa ctgggaaaga aaaaaagggg
      481 tatgatataa taaacgaaga agttaaacga tgaaaggagg agaaaaaatg aataaaaatc
      541 gttttctcca gggaagagga aagctggtta tcctggttct gcttgctctc cttcttggag
      601 gaagtacgtt ggcaggagct caacctcgga tctccatccg ttttcagctt ctacccgatg
      661 tgtatatttc cattcctcgc tcgccgcaga ttactctatc tatagataaa ggagaagggg
      721 caaactatta tatagatgac cctatcacca ttcgctatcg agcaaatcgg gacggttatg
      781 ttaatcttat agactattta cccgatggtg gggtaagcat tttagtccgt gatgaattca
      841 ttcgctctgg agaagagggg agattcgcag ggaaggtata tggtccggcg ggagtggaga
      901 gaatagtggt tcttctcacc cccgacaaag tgagtgaaag agacttggag agctttataa
      961 agtctcctca ccaagcagat agaatttttg gcactcgcta tgccactaac cgtatccact
     1021 tccaggtgct ggcgcggatg gaaagcacag ttttgacaat agagcctgac gatttaacta
     1081 tggatccggg gacttctgtg gttttaactg ctaccctaaa ggatgtaaga ggaagaccca
     1141 taacgggtga ggagattcgc tggtcggcga atgccgggcg cttgagcacc tcccgtacta
     1201 ccacagacct ccggggtcag gggagagtaa ctttctatgc tccctctcaa cccgatacag
     1261 taacaataga agcccgtttc ccgggaggag taaattcagc ttctgcttct gcccgggcta
     1321 cgattcgggt agaaagaaag acatattaca caactctcga ggtagctcct tcgtctttta
     1381 ccatcgatgg aggagggaac ataaagcttg tggccacctt aaaagatgag aatgggaagc
     1441 ccgtatacgg ccggactatt tactgggagg taaccgaagg agacctggat aaatactcta
     1501 ctcctactga ttcttccggt aaggctgtag tttattacta tgctccggaa gtggacgaag
     1561 ttaccgatgt gaaaataact gcccagtttc gaggagtagg tggactggct ccggcttctt
     1621 ttgaggtctg ggggacaata gagcctctac cggttgctct tacatcttcc atcttttatc
     1681 tcgactttgg tagcggcact ccccggggta acatagatag gctgcgctac tcaggggata
     1741 tcgttctggg tttctccttg aacgatttgt atgttttaga aatgaaacct cgagaagcga
     1801 tagagtttga gtttaatcct ggggggtctc ctcgagaagg agccattttc ctctggttgc
     1861 aaggagagtc aggaggaaag gcccgggttt ccgtaaacgg aaaccgctta gcaactatca
     1921 gcgtaagtcg ggacttaata gctccgggcg gagaagagaa aattattatt cctcgaaatg
     1981 tactggtgcg gggaatcaac cgcctgcgaa tagaagcaga tcccggtcgg aacattcgcg
     2041 tgcagaaggt aattattgtt ttttagcgct gtttgaagat agcatcaaat aagccctctc
     2101 cccgagaggg cttatttttt tcctcctatt ataagacccc tacaccgggg catccttcct
     2161 caagtcagag tgctttttct ccaggttgtt tttgatcttt ttctttttgt ctttttgtgt
     2221 tttggagact ataggagttg attcactctc accctctttc tctcatcatg cctgagcgct
     2281 tttatcaggc atccagagat ttttgccctt aaataatttg gattcctgat agaaccattc
     2341 aggaatgacc cccttttttg tcattcccga aatcttttat cgggaatcca
//
LOCUS       DAOPSS010000170         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_35725_length_2361_cov_9.705117, whole genome shotgun sequence.
ACCESSION   DAOPSS010000170 DAOPSS010000000
VERSION     DAOPSS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35725_length_2361_cov_9.705117"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..78
                     /locus_tag="PKN31_07850"
     tRNA            4..78
                     /locus_tag="PKN31_07850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Ile,seq:cat)
     gene            99..1199
                     /locus_tag="PKN31_07855"
     CDS             99..1199
                     /locus_tag="PKN31_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOA99869.1"
                     /translation="MERLETVIGFLEAIFPSSLAFPSDPTGLQIKGQDQVEKILVALE
                     LNPRVFRQAITENYDFLYLHHPPIWEPITSLSIDDPWLSMLGELYHQKVSILAHHTNL
                     DSAKNGLAEQWIKILSLEGKIQPLEPHSLRQFKVVTFVPPSHRDKVLEACFSSGGGKI
                     GNYEECSFLLSGKGTFRPQEGAHPFLGKVGEREEAEEIRIEVNVEESFLPQVVENIFS
                     SHPYEQPVVDVYPLFSSGDTGLGRMVTLSSPVSQEELEKRISLAFPLVAHYPDIDFPI
                     HYRKIALCPGSGKSLLERVIKEKPQAFLSGDLSHHDIARLNLWGITYYSIPHEEGERK
                     ALKEIYKYLKEEVDKQGLKVELNFETEEKRNE"
     gene            1192..1896
                     /locus_tag="PKN31_07860"
     CDS             1192..1896
                     /locus_tag="PKN31_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOA99870.1"
                     /translation="MSEILETLLALQDIDLKIDSEKRKIIKKEEEIVDINKQLEELKA
                     NLEEKKHNFEKARLQLAREELELKELEEKLKAAKNKLYSGEISSPKELSQWEKTMEKL
                     EESKSSLEDSVITSLENAEELQEEIQEKTQFYSQKEKNLLLLLNQLQEELETIRKSIS
                     EKEREREKIIASLPQEIVSTYEELRRKFPDPVTYLSGETCEGCNLTVPLAAVKAVRKG
                     EELVRCPNCGRFLNRK"
     gene            1871..2317
                     /locus_tag="PKN31_07865"
     CDS             1871..2317
                     /locus_tag="PKN31_07865"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOA99871.1"
                     /translation="MVDSLTESKELIAFIDGASKGNPGESSYAFILGNEEGTIIMETA
                     ARIGIATNNEAEYRALLAVLDQVLERGGKKLRVFSDSQLLVKQINHLYQVKSSRLLPL
                     YQEAQRKIHKLGHFEIFHIPREENQKADRLASEILTQKLSFKIGRK"
BASE COUNT          786 a          480 c          512 g          583 t
ORIGIN      
        1 tgagggccca tagctcagcg gtagagcagc cggctcataa ccggtcggtc cctggttcga
       61 atccgggtgg gcccaccatt ctatttgatg gagagaagat ggaaagatta gaaactgtaa
      121 ttggcttcct ggaagcgatt tttccttcct ctcttgcttt cccttctgat cccactggtt
      181 tgcaaattaa agggcaagac caggtagaaa aaatactggt agctttagag ctaaatcccc
      241 gggtatttcg tcaagcgata acagaaaact atgattttct ctatcttcat catccaccca
      301 tttgggagcc tattacttct ctttccattg atgacccgtg gctatctatg ttaggagagc
      361 tttatcacca aaaagtttcc atccttgccc atcataccaa cttagatagt gctaaaaatg
      421 gcctggccga acagtggata aagattttat ctctggaggg aaaaatacaa cctctcgaac
      481 cccattccct tcggcaattt aaagtggtta cttttgtccc tccttctcac cgagataaag
      541 tactagaggc ctgtttttct tctggagggg ggaaaatagg caactatgaa gaatgtagtt
      601 ttcttctctc cggaaaagga acttttcgac ctcaagaagg agctcaccct tttttgggaa
      661 aagtgggaga aagagaagag gcagaagaaa tcaggataga ggtcaacgtg gaagagagtt
      721 ttttgcctca ggtggtagaa aacatttttt cttctcatcc ctatgagcaa ccagttgttg
      781 atgtctaccc tcttttttcc tccggagaca cgggtttagg aaggatggtt actctctctt
      841 cccccgtttc ccaggaagag ttagaaaaga gaatctctct tgcttttcct ctggtagctc
      901 actatcccga cattgatttt cccatccact accgaaagat agctctttgc cctggaagcg
      961 ggaaaagtct tttagagaga gtgataaaag aaaaacctca agcttttttg agcggagacc
     1021 tctcccatca tgatatagcc aggctaaacc tgtggggtat aacttattac tctattcctc
     1081 acgaggaagg agaaagaaaa gctttaaagg aaatatataa atatctgaaa gaggaagttg
     1141 ataaacaagg attaaaagta gagttaaatt ttgaaaccga ggaaaaaaga aatgagtgaa
     1201 attttagaga cccttctggc tttacaggat atcgacctca aaatagacag cgagaagaga
     1261 aaaataatca aaaaagaaga agaaatagtc gatattaata agcaattaga agaactaaaa
     1321 gccaacctgg aagagaagaa acataatttt gaaaaagcac gcctccagtt agcccgggaa
     1381 gagctggagt tgaaagagct ggaagaaaaa ttaaaagccg ccaaaaataa actctacagc
     1441 ggggaaatca gctctcccaa agaactgtct cagtgggaaa aaacaatgga aaaattagaa
     1501 gaaagtaaaa gctccttaga agatagcgtt atcacttctt tagaaaacgc tgaagaatta
     1561 caggaagaaa ttcaggaaaa gacccaattt tattcccaaa aagagaagaa ccttctcctc
     1621 ttgctcaatc agttgcagga agaattagaa accataagaa aatcaatttc agaaaaggaa
     1681 agagaaagag aaaaaataat cgcttctcta ccccaggaaa tagtttccac ttatgaagaa
     1741 ttgagaagaa aattccctga cccggtcact tatttaagtg gagaaacctg cgaaggatgc
     1801 aacctaaccg tcccattagc tgcggttaaa gctgtgcgta agggagaaga attagtacgg
     1861 tgccccaact gtggtcgatt ccttaaccga aagtaaagag ctcatcgctt tcatcgatgg
     1921 agcatctaaa ggaaaccccg gagagagctc ttatgctttt atattgggca atgaagaggg
     1981 aactatcatc atggaaaccg ctgcccgaat cgggatcgcc accaacaatg aagcagaata
     2041 tcgagctctg cttgcagtgc tggatcaggt tttagagcga gggggaaaaa aacttcgagt
     2101 tttctccgat agccagctcc ttgtaaaaca aataaaccat ctatatcagg taaagtcttc
     2161 tcgcttgctt cctctttatc aagaagcaca gagaaaaatc cacaaactcg gccattttga
     2221 gattttccac ataccccggg aagaaaatca aaaagctgac cgtctggctt cggaaatatt
     2281 aactcagaaa ttgagtttta aaatagggag aaagtaggcc ggacaaccgc gggagaaatc
     2341 ctgaggaaag tccgagctcc a
//
LOCUS       DAOPSS010000171         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_37697_length_2264_cov_8.671797, whole genome shotgun sequence.
ACCESSION   DAOPSS010000171 DAOPSS010000000
VERSION     DAOPSS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37697_length_2264_cov_8.671797"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1259)
                     /locus_tag="PKN31_07870"
     CDS             complement(72..1259)
                     /locus_tag="PKN31_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOA99872.1"
                     /translation="MTSAEIIEKEARYFLGTYNRLPVVLERGEGCYVFDLEGKRYLDL
                     MAGIAVNVLGYSFPPLQEAIKREVDKLLHASNLYYSVPQVELAERLVEKSGFSRVFLV
                     NSGAEANEVALKMARFYRKRKYGKGYKFISFYRSFHGRTLLTLALTAQEKFHQDLDPF
                     PPGIEYATLNDLQSVEKVLDEEVCAIIVEPVQGEGGIYPCEREFLEGLRKLCDEKDVI
                     LIFDEVQCGIGRVGKFFAFESFGVRPDIVTLAKGLGGGLPLGAVLVDEKVSASVHRGD
                     QGSTFGGNPVCARSACVVVDEVSEENFLAAVREKGKYFEKQLKKLVSTFPIAKEVRGK
                     GLMLGFEVPGKAKKVAEEMLKEGVLVNACNDDVVRFLPPLIINQEEIDEGVEALKKVL
                     ERI"
     gene            complement(1423..2220)
                     /gene="argB"
                     /locus_tag="PKN31_07875"
     CDS             complement(1423..2220)
                     /gene="argB"
                     /locus_tag="PKN31_07875"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOA99873.1"
                     /translation="MKIVVKIGGKNIDFKNEDYFRVLAQLVKEGIRLALVHGGGPQIT
                     KFLEKEGRESQFVEGLRVTPPQDMEITEMVLSGLLNKMLVGIFHRQGIPACGISGRDG
                     FLLEGEKYYPASGDRKVDLGRVGEVKKVNPRLVHVLWEGGFLPVISPIAADREGESLN
                     VNADWAAARLAVALQADKLFLFSDVPGVLRDVEDRDSLIEVLSWAEIEILKEEGKIRG
                     GMIPKIEMAEEALRGGVKEVFIGEGKEMENLPLFLAGGKMRGTKVVL"
BASE COUNT          622 a          630 c          316 g          696 t
ORIGIN      
        1 gtatccaatc ctcctgaata agctaaaact actttctgtc gacccatttt aggtcctcct
       61 tcttcataaa actatattct ttctaaaact ttcttcaatg cttctactcc ctcatctatt
      121 tcttcttgat tgataattaa agggggtaaa aacctcacca cgtcatcatt acaggcattg
      181 actaaaactc cctctttgag catctcctca gcaacttttt ttgcttttcc gggaacctca
      241 aaacctaaca tcaagccttt acctcgaact tcttttgcta ttggaaaagt agaaactaac
      301 ttcttgagct gtttttcaaa atattttccc ttttctctta ctgcagcaag aaaattttcc
      361 tcgcttacct catctactac cacacacgct gaccgcgcac acaccgggtt ccccccaaaa
      421 gtgcttccct ggtctcctcg atgaactgaa gcagaaactt tttcatcaac taaaaccgcc
      481 cccagaggca agcctcctcc caagcctttg gctagagtaa ctatatccgg cctgactcca
      541 aaactctcaa aagcaaaaaa ttttcccact cttcctatac cacattgcac ttcatcaaaa
      601 atcaaaatca cgtctttctc atcacataat tttctcaaac cctctaaaaa ttctctctca
      661 cagggataaa ttcctccctc cccctgtact ggctccacta tgatggcgca tacctcctca
      721 tctaaaactt tctctacgct ttgcaggtcg ttaagagtag catattctat ccccggagga
      781 aaggggtcta aatcctgatg aaatttttct tgagcagtga gagctaaagt caaaagagtt
      841 cttccatgaa aagagcgata aaaggagata aacttatagc ctttaccata tttcctcttg
      901 cgataaaaac gggccatttt caaagctacc tcattggctt ctgcaccgct gttaactaaa
      961 aacacccgag agaaaccaga tttttccacg aggcgttctg ctaactctac ctgaggtaca
     1021 ctataatata gattagaagc gtggagtaat ttatccactt ctctttttat agcttcctgg
     1081 agagggggga aagagtaacc taaaacgttt actgcgatcc cggccatgag gtcaagatat
     1141 ctttttcctt ctaaatcaaa gacgtagcat ccttctcctc gctccagtac caccggtaaa
     1201 cgattgtaag tccccaaaaa atagcgtgct tctttttcta tgatttctgc agaagtcatt
     1261 tacctcacct tctttcttgt actgagaaag acttcgtctt cctcagttgt ttttctctac
     1321 attttcatgc ttttaatctt acctcatagg ctgtctgttt tttgcttttc ccgagggaga
     1381 ataaggggac aaagccctct tattctccct tgcagactgc ctttaaagca ccaccttcgt
     1441 tcccctcatt ttcccccccg ctaaaaagag aggaaggttt tccatctctt ttccctcgcc
     1501 aataaatacc tcttttactc caccccgtag agcttcttca gccatctcta ttttgggaat
     1561 cattcctccc cgaatcttcc cctcttcttt caggatttca atttccgccc aggacaaaac
     1621 ttctatcaga gaatctctat cctccacatc ccgtaaaact ccaggaacat cagaaaagag
     1681 gaacaactta tcagcttgca aagccacagc caagcgagct gctgcccaat cagcatttac
     1741 atttaagctc tctccttctc tatcagctgc tatcggagaa ataacaggca aaaatcctcc
     1801 ctcccacaaa acgtgcacca ggcgaggatt cactttcttt acttccccta cccttcccaa
     1861 atctaccttt ctatctccag aggcagggta atacttttcc ccttccagca aaaaaccatc
     1921 tcttccgctt attccacaag caggaattcc ctgacgatga aaaataccta ccaacatttt
     1981 atttaaaaga ccgctcaata ccatttctgt tatttccatg tcttggggag gagtaactcg
     2041 caaaccttct acaaactggc tttctcttcc ttctttttct aagaatttag taatctgagg
     2101 ccctcctcca tgcactaaag ctaatcttat cccctctttt actaattggg caagcactcg
     2161 aaagtagtcc tcatttttaa aatcgatatt tttgcctcca atttttacca ctatcttcat
     2221 agtttcctcc tcctgattta caggaaataa taattaatca caaa
//
LOCUS       DAOPSS010000172         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_38713_length_2217_cov_7.901480, whole genome shotgun sequence.
ACCESSION   DAOPSS010000172 DAOPSS010000000
VERSION     DAOPSS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38713_length_2217_cov_7.901480"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..486
                     /gene="rpsO"
                     /locus_tag="PKN31_07880"
     CDS             217..486
                     /gene="rpsO"
                     /locus_tag="PKN31_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOA99874.1"
                     /translation="MALTKERKQEIIEQFRIHEADTGSPEVQIAILTERIKDLTEHLK
                     THPKDYHSRRGLFLMVGQRRRLLNYLKKYHIDRYYAILERLGLRR"
     gene            494..>2217
                     /locus_tag="PKN31_07885"
     CDS             494..>2217
                     /locus_tag="PKN31_07885"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOA99875.1"
                     /translation="MFNITKQFEISFGKESLLIETGKLAKQAGGAVTVKYGGTVVLVT
                     ATAAEEPKEDIDFTPLLVEFEERFYAAGKIPGGFFKREGRPGQGAILNARLVDRSIRP
                     LFPELFRNDIQVVVTVLSVDETHPPDAISILGASLALGISDIPFDGPLAACRMGLVGD
                     ELVINPNLEELSSSKLDMIIAGSQEGITMIEAGAQEVQESQVAQAMMLGYQEIVKAIK
                     AQEDIISQIGKTKREIVIPPYFNDLSSWLEDSYRASIREVITIPEKTEREKETRLLMK
                     KAQEEAMEKFESISLFTMAWERIMGEEVKELILTSERRQDGRTPKEIRPVSCEAGFLP
                     RTHGSSLFTRGQTQALVITTLGASSEEQKVDALQDEEAKRFMLHYNFPPYSTGEVRPM
                     RGPGRREIGHGALAERALECFIPPEGEFPYTIRVVSEILESNGSSSMATVCGGSLSLM
                     DAGVPVRSACAGLSIGLVKSDEKYLLLRDILGSEDHYGEMDFKVAGSRNGITDIQLDV
                     KNRGLSWEILGQALEEAKEGRFYILDIMDGAIDRPRSSVSPYAPRIEILDINPDRIGA
                     LIGPGGKV"
BASE COUNT          645 a          398 c          571 g          603 t
ORIGIN      
        1 ggaagtccat cctgaaattt ctcgcagaat gcgagaaagc aaaggctaaa gcttgttgga
       61 accggtggct tttttgtttg gttttgtaac ctgcttgaaa gtttctgctt ttttaagctt
      121 ataagtttac ttcttcaggg ggttagccag ctatatttac agtaaagtcg gggtgtggta
      181 aactactgaa taagtataaa tgggaggtgt agatgcttgg ctttaactaa agaaagaaaa
      241 caagaaatca tagaacaatt cagaattcat gaggcagata ctggatctcc tgaggtgcag
      301 attgctatcc ttactgagag gattaaagat ctcaccgaac atctgaaaac ccatcctaaa
      361 gattatcatt cccgacgggg gctctttttg atggtaggac agagaaggag attattaaat
      421 tatttgaaga aataccatat agatcgctat tatgctattt tagaaagatt aggattgaga
      481 agataaaggg aggttgttta atataacgaa acaattcgaa attagttttg gtaaagaaag
      541 tctgttaata gagacaggga agttggctaa acaagctgga ggagcggtga cagtcaaata
      601 tggaggcacc gtggtgctgg tgaccgctac tgctgcagaa gagcctaaag aagatattga
      661 ttttacaccg cttcttgtag aatttgaaga acgtttttat gctgcgggta aaatccctgg
      721 agggttcttt aaaagagaag ggaggccagg acagggggct attttgaatg cccgtctggt
      781 agaccgttct atcagaccac tttttcctga gcttttccgc aatgatatcc aggtagtggt
      841 tactgttctt tcggtggatg aaactcatcc tcccgatgcc atatctatct taggtgcttc
      901 tttagcttta gggatttccg atattccctt tgatggccca ttagctgcct gccgtatggg
      961 actcgtggga gatgaattgg taattaaccc taacttagaa gagttgtcct ccagcaagct
     1021 ggatatgata atagcaggct cccaggaagg gattaccatg attgaagctg gagctcaaga
     1081 agtgcaggaa tcacaggtag ctcaggctat gatgctgggc tatcaggaaa tagtcaaagc
     1141 cattaaagct caagaggata ttatttctca gatagggaaa actaagaggg aaatagttat
     1201 tcctccttat ttcaatgacc tttcttcctg gctggaagat agttatcgtg cttctattag
     1261 agaggtaatc actattccgg agaaaaccga gcgagaaaaa gaaacaagac tcctcatgaa
     1321 aaaggctcaa gaagaagcga tggagaaatt tgagagcata tctcttttca ccatggcttg
     1381 ggagcgaatc atgggagagg aagttaaaga attaattttg acaagtgaaa gaagacagga
     1441 tggtcggact cctaaagaga taagacctgt cagttgtgaa gcagggtttc tacctcgcac
     1501 tcatggttcc agtctcttta cccggggtca aactcaggct ctggttatca ccaccttagg
     1561 agcctcgagc gaagagcaaa aagtagacgc tcttcaggat gaggaagcca aaaggttcat
     1621 gttgcactat aattttcctc cttacagtac gggagaagtc cggcctatgc gaggtccggg
     1681 gcggagagaa atcgggcacg gggctctggc agaaagagct ctggagtgtt ttatccctcc
     1741 cgagggggag tttccttata ccataagagt ggtttcggaa attttggaat ctaatggttc
     1801 ttcttccatg gctacggttt gtggaggaag cctgtcgctg atggatgcag gagttccggt
     1861 tcgatctgct tgtgccggtt tatccatagg tcttgttaaa tcagacgaaa agtaccttct
     1921 cttacgagat attttaggtt cagaagacca ttatggagag atggacttta aagtagcagg
     1981 aagtagaaac ggtataaccg atatccagct agatgtaaag aacagaggtc tgagctggga
     2041 aatactgggg caggctctag aagaggctaa agaagggcga ttttacattc tggatatcat
     2101 ggatggagcg atagaccgac ctcggagttc tgtatctcct tatgctcccc ggatagaaat
     2161 tctggatatt aaccccgacc gcattggtgc cttaattggt ccgggaggaa aagtcat
//
LOCUS       DAOPSS010000173         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_40679_length_2132_cov_7.980260, whole genome shotgun sequence.
ACCESSION   DAOPSS010000173 DAOPSS010000000
VERSION     DAOPSS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40679_length_2132_cov_7.980260"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="PKN31_07890"
     CDS             complement(<1..2071)
                     /locus_tag="PKN31_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015591480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOA99876.1"
                     /translation="MEENRKIKIPGMLYILISFIPWIIYWILCSMGNTLGVLIPLIIS
                     LILINPQVQRRDFNLMDITSLLYFSIANVATFIFNVSVFMEQNGFMGYFVLFLMALIS
                     IIVKQPYTLQVSKRDYPEVYWKDKSFLAINNLITAVWAGIFLSNAVIFLFFSFPFTVI
                     LSNVLVALGIIFSTIFPIKAPAYFTTKEFKRYDWNVRVEPKIPKGENEYDVIIVGSGI
                     AGLVCGALLSRRGYKVLVMEQHSQVGGYCSSFQRGGFVFNTGVEDVSGLWERGPVTRL
                     LEVLGLKREDLFVRNTTRYILKGEEISANNLEEFTNALSSIHPEEKDNISSFFSDAER
                     AYIECYQGTEIYGVPLPAELIVKVWGERELLDYPRKCPHFYDWMNKTYIEKLDEYFND
                     EDLKYLMSALLGYVGTEPEKTPASSALTACVSYYLHGGYFPKGGAQRFANTLRDFVEE
                     QGGEVLTRHKVDKIVVEKGKVTGVRRGENTFEAPIVVANANAKTTFLELVGEDILDKE
                     FTRYIKGLKMSPSAFMVFLGADVDLSSYPTLIKNLDEGYEIIINSNADPTLAPDGKSS
                     ITLIRGANYHDFPERGTEEYVKKKEEMKEMLIQKAEKVIPELKGHIVVQDAATPKTLE
                     RYTLMPEGALYSFDQSVEVKRPYFKTPLKGLYLASASTFPGGGIEAVVMSGIICANDI
                     CGWEGGLL"
BASE COUNT          653 a          500 c          340 g          639 t
ORIGIN      
        1 atagcaatcc accttcccac ccacatatat cattggcaca aataattccg gacattacca
       61 ctgcctctat tccccctccc gggaaagttg aggcacttgc taaatacaga ccctttaagg
      121 gtgttttaaa atagggtctt ttaacttcta cggactggtc gaaggaataa agagcgcctt
      181 caggcatcaa ggtatatctc tccagagttt ttggcgttgc tgcatcctga acgactatat
      241 gccccttaag ttcaggtata accttctccg ctttctgaat tagcatctct ttcatttctt
      301 ctttcttctt tacatactct tcagttcctc tttctggaaa atcgtggtag tttgctcctc
      361 ttatcagggt aatactggat ttaccatctg gtgccaaagt tggatcagca ttagaattga
      421 tgataatttc atacccctca tctaaattct taataagcgt aggataactc gataaatcca
      481 catcagcacc caaaaatacc ataaaagcag agggcgacat ctttaatcct tttatatacc
      541 tggtaaactc cttgtccaaa atatcttctc ccaccagctc taaaaaggta gttttagcgt
      601 tggcattagc aacaactatg ggggcttcaa aagtattctc tcctcttctt acccctgtaa
      661 ccttaccttt ttccaccact attttatcta ctttgtgcct ggttaaaact tctcctcctt
      721 gctcttcaac aaaatctctc aaagtattgg caaaccgttg tgcaccacct ttagggaagt
      781 aacctccatg gaggtaatag gaaacacaag cggtaagtgc gctgctggca ggtgttttct
      841 ccggctctgt ccccacataa cccaggagag cgctcatcag atattttaag tcttcatcat
      901 taaagtattc atcgagtttc tctatatagg tcttattcat ccagtcataa aagtgaggac
      961 actttcgagg ataatctaaa agttccctct ctccccaaac tttcacgatt aattctgcag
     1021 gcaagggaac accataaatt tctgtaccct ggtagcattc tatataagct cgctcggcat
     1081 cactgaagaa ggaagaaata ttatcttttt cttcgggatg aatgctggag agcgcatttg
     1141 taaactcttc taaattgtta gcgctaattt cctctccttt caaaatatat cttgtagtat
     1201 ttcgcacaaa taagtcttct ctttttagac ctaatacctc caaaaggcgg gtgacaggac
     1261 ccctctccca caacccactt acatcctcca ccccggtatt aaaaacaaac cctcctcttt
     1321 gaaaagaaga acaataacca cccacctgag aatgctgctc cataaccagt actttataac
     1381 ccctccgaga gagcaaagca ccacacacca agccagcaat acctgagccg acgattataa
     1441 catcatattc attctcccct tttggaattt tcggttctac tctcacattc cagtcgtatc
     1501 ttttaaattc tttagtagtg aagtaagcag gcgctttgat aggaaaaatt gtggagaaaa
     1561 ttatgcccag ggcaaccaga acattggaca atataacggt gaaaggaaaa gagaaaaata
     1621 ggaatataac cgcattagat agaaaaatac cagcccaaac cgcagttatc aggttattta
     1681 tagctaaaaa agatttgtcc ttccagtata cttctggata atctcttttt gatacctgaa
     1741 gagtataagg ttgttttaca attatggaaa tcaaggccat caaaaataga acaaaatatc
     1801 ccataaagcc attctgctcc ataaatacac tcacattaaa gataaaagta gcaacattag
     1861 ctatactaaa atagagaaga gaagtaatat ccatcaaatt gaagtctcgt ctctggactt
     1921 gaggatttat gagaattaaa gagattatta aggggataag cactcctaaa gtatttccca
     1981 tgctgcacag tatccagtaa ataatccagg ggataaacga aatgagtata taaagcatac
     2041 ccggtatttt tatttttcta ttttcttcca tttccctctc cttttttatc tattatactc
     2101 aaccgcaaaa tccacggtaa tctttcttcc cc
//
LOCUS       DAOPSS010000174         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_41915_length_2083_cov_8.040927, whole genome shotgun sequence.
ACCESSION   DAOPSS010000174 DAOPSS010000000
VERSION     DAOPSS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41915_length_2083_cov_8.040927"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKN31_07895"
     CDS             complement(<1..416)
                     /locus_tag="PKN31_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442363.1"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HOA99877.1"
                     /translation="MSTERPSRPKMPQRPPEERIKDFNEVPLGLSEEVAVAEARRCIQ
                     CKKPTCIEGCPVEIDIPGFIKLLSQGELDGAIAKIKEKNSLPAICGRVCPQEDQCESK
                     CILGRRGQPVAIGYLERFLADYEREKGVKAPSLPPPR"
     gene            complement(406..1260)
                     /locus_tag="PKN31_07900"
     CDS             complement(406..1260)
                     /locus_tag="PKN31_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOA99878.1"
                     /translation="MAKILKKEVLTPQIKLMVIDAPWVARKARPGQFVVLRINEQGER
                     IPLTIADFDLQEETVTIIFQEVGKTTMVLGDLEEGDEILDFIGPLGKEIEEKYFGHVV
                     CVGGGVGIAPTYPKARAFKEKGNRVTSIIGARSSNLLFWEEEMRRVSDELFVTTDDGT
                     YGEKGFVTQVLERVLNKDKVDLVVAVGPVIMMKMTSLLTEKYSIPTLVSLNPIMVDGT
                     GMCGCCRVTVGGKCKFTCVDGPVFDGHQVDFDELMARQRIYLEEEKRALDAWLMETEK
                     EGAPYHVY"
     gene            1413..1943
                     /locus_tag="PKN31_07905"
     CDS             1413..1943
                     /locus_tag="PKN31_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOA99879.1"
                     /translation="MKKLRFISVSLVAILSIFLAVGVASAQAISSQPVYYAKFMDTVS
                     QVEELSTFVEVVKAAGLEETLNKGEYTIFAPTNDAFDALPEGKLERLLQPENKETLKD
                     ILTYHVIPRRLSVDDFRAGEKYKTLNGHLIHVFFEEPPIVINRKINVVGSYAASNAII
                     YMVDKVIFPPEGPKPK"
     gene            complement(2007..2081)
                     /locus_tag="PKN31_07910"
     tRNA            complement(2007..2081)
                     /locus_tag="PKN31_07910"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2047..2049),aa:Thr,seq:cgt)
BASE COUNT          590 a          510 c          376 g          607 t
ORIGIN      
        1 cgaggaggag gaagagaagg agctttcact cccttttctc tttcataatc agctaaaaaa
       61 cgctctaagt atccaatggc aacgggttgt cctcttctgc ccagtataca cttgctttca
      121 cactggtctt cctggggaca cactcttccg catatagcag gaaggctatt tttctccttt
      181 attttagcaa tggccccatc caattcaccc tgcgatagaa gtttaataaa accaggaata
      241 tcaatttcca ccggacaccc ttcgatacag gtgggttttt tgcactgaat gcaacgacga
      301 gcttctgcta ctgctacctc ttccgatagc cctagaggaa cttcattaaa atctttaatc
      361 cgctcttcgg ggggacgttg aggcatttta ggtcgagaag gacgttcagt agacatggta
      421 gggggctccc tccttttctg tttccattaa ccaggcatct aacgctcgtt tttcttcctc
      481 taaataaatc cgctgacggg ccatcaattc atcaaagtct acctggtggc catcaaaaac
      541 tggaccatcc acacaagtaa atttacactt tccccccact gttacccgac agcaaccgca
      601 cattcccgtt ccatctacca ttatgggatt aagactcacc agagtgggaa tggaatactt
      661 ttcagttaag agactcgtca tcttcatcat tattaccggc cccaccgcta ctaccaaatc
      721 caccttatct ttatttagaa ctcgttccag aacctgagtt acaaatcctt tttcaccata
      781 tgtcccatcg tcagtagtaa caaaaagctc atcactcact cttcgcattt cctcttccca
      841 gaaaagaaga ttagagctgc gagctccgat gatagaggta actcgatttc ccttttcctt
      901 aaaagctcga gccttaggat aagtgggggc aatacccacc cctcctccta cacaaactac
      961 gtgaccaaaa tatttttctt cgatttcttt tcctaaaggt cctataaaat ctaaaatctc
     1021 atccccttct tctaaatccc ctaacaccat agtggtttta cctacctctt gaaaaattat
     1081 ggtaactgtt tcttcttgca gatcaaaatc agcaatagtt aagggaatac gctccccctg
     1141 ttcattgatg cgtaaaacca caaattgacc gggcctggct ttgcgagcca cccaaggagc
     1201 atcaattacc atgagtttaa tctgaggggt gaggacttct ttttttagaa tctttgccac
     1261 tttctttcct ccttctgagt ttagaaaacc agaaaaaatt atactatata atccttcttt
     1321 gtttagggta tattttagac cataccgttt tattttccaa atcctatgtt ataatgtttc
     1381 ccagtaaaat aaaaggaaag gagaaggtca aaatgaaaaa attgcgtttt atctctgttt
     1441 cacttgtagc tattcttagc atatttctcg cggtaggtgt tgcatcagct caagccattt
     1501 catctcaacc tgtctactat gctaaattta tggacaccgt ctctcaagtg gaggagttga
     1561 gcacttttgt cgaggtagta aaagctgctg gattggagga gactctcaac aagggtgaat
     1621 ataccatttt tgctcccaca aatgatgcct ttgacgctct tccagaagga aaactggaaa
     1681 gattgctcca gcccgagaac aaagaaactt taaaagatat actcacttat cacgtaattc
     1741 ctcgtcgtct ctcagtagac gattttcgag caggggaaaa gtacaaaacc ctgaacggcc
     1801 atttaatcca tgtctttttt gaggaaccgc ccatcgtgat taatagaaaa atcaacgtgg
     1861 taggatctta tgctgcttct aacgctataa tctatatggt ggataaagta attttcccgc
     1921 cagaaggacc caaaccaaaa taaaggtgaa tactaaaaag cgcccctttt caaaaataaa
     1981 gtttaggggc gcttacaatt tttagatgga gccggcgagc ggactcgaac cgctaacctg
     2041 cgcattacga atgcgctgct ctaccattga gccacaccgg ctt
//
LOCUS       DAOPSS010000175         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_41968_length_2081_cov_8.008391, whole genome shotgun sequence.
ACCESSION   DAOPSS010000175 DAOPSS010000000
VERSION     DAOPSS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41968_length_2081_cov_8.008391"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1335)
                     /locus_tag="PKN31_07915"
     CDS             complement(298..1335)
                     /locus_tag="PKN31_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOA99880.1"
                     /translation="MKAIVKIRKEKGLELKEMEVPRPGKGEVLVEIKAAAICGSDIKI
                     YKWDSFAQSIIHSLPFIPGHECAGEVVEVGEGVKSIRLGDKVASETHIPCGQCWQCTH
                     GRPHTCENMKLFGHNINGCFAEYAVIPEVSTRKIPSSLTFEEGCMLEPMGIPYRAVEK
                     GKVEKEAVVVVGCGPIGQFAIGFSHVMGAGQIIAVDVNEERLKIAEAMGAHYLLNPEK
                     EKVKERIKELTQDWGKGPGVVIEASGNVSALREALDYLRVGGKLLILGQSTNPLEVRV
                     SPDIVFKEAEIYGFFGREIWDTWEKTEKLLVEGKMKVDPVITHRFSLEDYEIAFEKAA
                     KGEGGKIVFLP"
     gene            complement(1345..>2081)
                     /locus_tag="PKN31_07920"
     CDS             complement(1345..>2081)
                     /locus_tag="PKN31_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOA99881.1"
                     /translation="LYHEARNYLISDVGGAVEGAIEGGADEVIIFDMHYSGLNLNLEE
                     IHPRAKVVMGKPRKIYPFFSELYQKIKGVMMVGFHAMAQAPGGLLTHSYDHSMKAIYL
                     NGILVGEIGMEAAMAGYYHVPLILVTGDSEGVKEAENLLGEIETATVKYAINEHSALC
                     LPLELTRKEIKEKAKKATENINRFSPFRINPPYAIEVEFFEKEGAQKMEGFPGLQKIN
                     EVKVRVEGENLPLLWESFIGFYQKYD"
BASE COUNT          574 a          543 c          340 g          624 t
ORIGIN      
        1 aggggtctat attatttact aagaacaaga acatctggat tcccgattac tgatttcggg
       61 aatgatggag gagaacaata agggggtgaa ccccctttat aacccccaaa aaaacttaaa
      121 aacccaagat tactgagggg agaaaaagcc tccctcagtg gattcccgat aaaagggctc
      181 gggaatgaca agtaagatag gacaaaactc gagatcacca cgtaaatgaa actacgagtt
      241 ctcaataacc atattcaaaa caaatattac cgtaagtttt gcagtcagct atactcctca
      301 aggaagaaaa acaattttcc ctccttctcc ttttgctgcc ttttcaaaag ctatttcata
      361 atcctctaag gaaaaacggt gagttatgac cgggtctact ttcattttcc cctctaccag
      421 taatttttct gttttttccc aggtatccca gatttccctt ccaaagaaac cataaatctc
      481 tgcttcttta aatactatat ctggagacac tctcacttct aaggggttag tagattgacc
      541 caggatgaga agcttgcccc ctacccggag ataatccagc gcctcacgca gagctgaaac
      601 gtttcctgaa gcctcaatca ccactcccgg tccttttccc cagtcttggg ttaactcttt
      661 tattctctct ttaacctttt ccttctcagg gttcaagaga tagtgagcac ccatagcttc
      721 agcaattttc agcctctctt cattcacgtc cacagcgata atttgacccg cccccattac
      781 atgagaaaag cctatagcaa attgaccaat agggccacac cccaccacca ctaccgcttc
      841 tttttctact tttccttttt ctactgcccg gtaaggtatc cccataggct ccagcatgca
      901 gccttcttca aaagtaagag aggaggggat ttttctggta gatacctcgg gaataaccgc
      961 atattcagca aagcacccgt tgatattatg accgaaaagc ttcatatttt cacaggtatg
     1021 aggtctacca tgagtgcact gccagcactg accacaggga atatgagtct ctgaagccac
     1081 cttatccccc aatcgaatac ttttcactcc ttctcccact tccaccactt ctcctgcaca
     1141 ctcatgcccg ggaataaaag ggagagaatg aattatactt tgagcgaaag aatcccattt
     1201 atatattttt atatctgagc cacagatagc cgctgcttta atttctacta agacttctcc
     1261 cttccctggc cggggaactt ccatctcttt caattccaat cctttttctt tcctgatttt
     1321 aacaatagct ttcatgttca ttcctcaatc atatttctgg taaaacccga taaagctctc
     1381 ccacaaaaga ggaagatttt ccccttccac ccggactttt acctcattaa tcttttgtaa
     1441 acccggaaat ccttccatct tctgtgctcc ctctttttcg aaaaactcca cttctatagc
     1501 ataaggaggg tttattcgaa aaggtgagaa tcgattaata ttttccgttg cttttttagc
     1561 tttttctttt atttcttttc tggttaactc cagaggtaaa cacagagcgc tatgctcatt
     1621 tatagcgtat tttaccgttg ccgtttctat ttctcccaga agattttcag cctcttttac
     1681 tccttctgaa tctccagtaa ctaaaatcag tggcacatga taataaccgg ccattgctgc
     1741 ttccatgcct atctctccca ccagaattcc gttcaaatag atggccttca tagaatggtc
     1801 ataagaatgg gtcaacaacc ctcctggagc ttgagccatg gcatgaaacc ccaccatcat
     1861 tactcccttt attttctggt agagctcaga aaaaaagggg taaattttgc gtggttttcc
     1921 catcaccacc ttagcccggg gatgaatttc ctccaggttc aagtttaaac cagaatagtg
     1981 catatcaaaa attataactt catccgctcc cccttctatt gctccttcca ccgctccacc
     2041 tacatcagaa ataaggtagt ttcttgcttc atggtaaaga g
//
LOCUS       DAOPSS010000176         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_42248_length_2071_cov_7.697421, whole genome shotgun sequence.
ACCESSION   DAOPSS010000176 DAOPSS010000000
VERSION     DAOPSS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42248_length_2071_cov_7.697421"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            496..1698
                     /gene="hisZ"
                     /locus_tag="PKN31_07925"
     CDS             496..1698
                     /gene="hisZ"
                     /locus_tag="PKN31_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOA99882.1"
                     /translation="MELVLGMKDSYPREVKRKREMEFVLRNLFEKWGYEEVEPPTIEY
                     YSTLENTLGDEAKERVYKFVDRNGKLVSLRPEFTTSIMRMYSKKEMRETAPHRVYYVG
                     KIFRYPSSPLRVEDELTQLGLESLGKNGVDTDAEIIALALSALLALQELGIEDFMLDI
                     GHVGIFQGVLSSFPLSPSEKLSLKSLLKKKDFVALEKFTSISSHPLFPFLLHLPFLRG
                     KREILEETKKNYPLSPPLIDTLNYLGEIWDIISDFHLEKHAFINLGLIRDFDYYTGVV
                     FEGFSPRLGYPLLAGGRYDELLEKSGSSLPACGFALFLERLMEVQKESIKKDRPPFIS
                     IAYPPVFRSSIFALAEKMREEGIPIIIEEQKGEDIQVLQGKELIYQGKDEEKIKTLIQ
                     ERFKSWES"
     gene            1686..>2071
                     /gene="hisG"
                     /locus_tag="PKN31_07930"
     CDS             1686..>2071
                     /gene="hisG"
                     /locus_tag="PKN31_07930"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOA99883.1"
                     /translation="MGKLTISIPTGRMKEESLALLSRLPQCPPLDFVNSRRLVVEQED
                     LPWRIIFSHPKDSSTYVEFGAADIGIVGKDILLERENEVYELLELPIGKCTMVLAAPL
                     STSREELLEKEALRIATTYPNFTRRYL"
BASE COUNT          622 a          391 c          453 g          605 t
ORIGIN      
        1 ggtatatcgt cttccctgat tgagtgggta gagagctggt tactatcttc tggcttcaga
       61 gtcagagggg tttatctcgg atgcaatagt tgtaacagtt gatagcccgg atgttaaaaa
      121 gctgcccttc tcgattttgg ttacgatcat aaatatgaat gtgaccatga acgcgatagc
      181 gaggttggtg tttttttgat gatgcgggta aaagcagaga acctccccga tgagcaaggt
      241 cgcttccttt atgaactcct tgaggggggg agcatgggct accttgaata gatcataacg
      301 acaatgctta atataaatag aaaactataa aaacatcaaa aaataatgtc actataaaat
      361 aaggaaaaaa tgggaaacag aaaaaccggg ggtgtaacca attaaaattt tctttcatca
      421 catatataat atgattcttt accattattt tgctaaaata gtttaaacaa ttcttcaatg
      481 agaggagata atggcttgga attagtttta ggtatgaaag attcttaccc ccgggaggta
      541 aaaagaaagc gggagatgga gtttgtcctg cgcaatcttt ttgagaaatg gggatacgaa
      601 gaagtggagc ctcctaccat agaatattat tctaccctgg aaaacacctt gggagatgaa
      661 gccaaagaaa gggtgtacaa atttgtagac cgaaatggga agttagtctc tctccgtcct
      721 gagttcacca cctccataat gcggatgtac agcaaaaaag agatgagaga aactgctcct
      781 caccgggttt actatgtagg gaaaatattt cgctatccct cttcacctct gcgggtagaa
      841 gatgagctta ctcagctggg cttagagagt ttaggcaaaa acggcgtaga taccgatgcc
      901 gaaatcattg ctcttgctct ctcggctctt ttggctcttc aggaattggg gatagaagat
      961 tttatgctgg atatcggtca tgttggtata ttccagggag tactatcttc ttttcctctt
     1021 tctccctcag agaaactctc tttaaaaagt ttgctcaaga aaaaggattt tgtagctctg
     1081 gaaaaattta cttctatctc ttcccaccct ctttttcctt ttcttctcca tcttcctttt
     1141 ttacgaggaa aaagagagat cttagaagaa acgaaaaaaa actaccctct cagtcctccc
     1201 ttaattgata ctttaaacta tctgggagag atatgggaca tcatcagcga ttttcacctc
     1261 gagaagcatg cctttattaa tttagggctt ataagagact ttgattatta taccggagta
     1321 gtatttgaag gtttttctcc ccggttaggg tatccacttt tagcgggagg aagatatgat
     1381 gaacttttag aaaaatcggg aagtagtttg ccagcctgcg gatttgccct gtttttggag
     1441 cgattgatgg aggtgcaaaa agaaagcatt aaaaaagacc gtcctccctt cattagcata
     1501 gcttatcctc ctgtctttcg ctcttcaatt tttgccctgg cagaaaaaat gagagaagag
     1561 ggaattccca taatcataga agagcaaaaa ggagaagaca tccaggtttt acagggaaaa
     1621 gagcttattt accagggaaa ggatgaagaa aaaattaaaa cacttatcca ggagaggttt
     1681 aagtcatggg aaagttaacc atatctatac ctaccggcag aatgaaagag gaatctttag
     1741 cgcttctttc tcgcttaccc cagtgtccac cccttgattt tgttaattcc cgacggttgg
     1801 tggtagagca agaagattta ccctggcgta tcattttttc tcaccccaaa gactcctcta
     1861 cttatgtgga gtttggggca gcagatattg gaatagtggg gaaagatatt cttttggaga
     1921 gagaaaatga agtttacgaa ttattagagc ttcctatcgg gaaatgtact atggttttag
     1981 ctgctcccct atctacttcc cgagaggaac tattagaaaa agaggccctg cgtattgcca
     2041 ctacctatcc taacttcacc cgtcgatatc t
//
LOCUS       DAOPSS010000177         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_42401_length_2066_cov_6.183491, whole genome shotgun sequence.
ACCESSION   DAOPSS010000177 DAOPSS010000000
VERSION     DAOPSS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42401_length_2066_cov_6.183491"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PKN31_07935"
     CDS             complement(<1..871)
                     /locus_tag="PKN31_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOA99884.1"
                     /translation="MVQKKIKRLFWGLVFLLVLGMGGMVYAQTVSPPVVAIRVECNEN
                     ISEQLILSAVYTSLKEPYNPEKVQADVKSIYDLGYFSKVWVETKEYPDGIELIFKVQE
                     FPPVKEINISGNTALSYDEIRKVMILSPGQIMNWKIFQHDLERIKALYTDNGFLITAV
                     EEINFSEEGVLSFTIKEGIVEAVEFEGLEKTKEYVLRRELTFEPPLVFDFSKVQESLR
                     AIYNLGFFEDVSMKLEPGSDRDHVIVKVKVEEKLTGEAGLGVGYNSERGWLGYIRYQE
                     SNLGGNAQRVELRY"
     gene            complement(892..>2066)
                     /locus_tag="PKN31_07940"
     CDS             complement(892..>2066)
                     /locus_tag="PKN31_07940"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOA99885.1"
                     /translation="SIPYGVLQLRGEANIAISGDEEKPLWQGEVEGEGEIWREGKKYA
                     DFNNVRGIIKEGEIQLGSGSLHIAGLNWIIEGVISREESEIHLSGGGKLEIPEENVGL
                     SRVESNVVIRACDGIINLQGEAKIFDGWGDFTKGGDTENYSSLLSQLEEKLEELPLSL
                     ELTLYTANKLRVKTRFLDLEMEGRLKIWGGSGRINGEGKLEVVEGNYDLVLRKIPIRG
                     YITFSPLYGLNPRVNLYGEEEIGGYHIFLEAMGPVDNYKLILKSEPLLEEEEILSLLF
                     LGEKDAYLSLDNLNWQPLLWKLGQFLLGKDFFSKGGLFDGVEIKFPGSDYSDFYGLRL
                     EKSIGENYFIGYTQDLSGEGNSSWDFKINFDKEWSLKMEGDTEGEINWMLEFNTKF"
BASE COUNT          618 a          523 c          264 g          661 t
ORIGIN      
        1 catatcttag ctccactcgt tgtgcatttc ctcctaagtt agattcctga taacgtatgt
       61 agcccaacca acccctctca ctgttatacc ccactcccaa tcctgcctca ccagtcagct
      121 tttcttccac tttgactttg actatcacat ggtctcgatc actacctggc tccaatttca
      181 tagacacatc ctcaaaaaag cccagattat aaatggctct caagctctcc tgaactttag
      241 aaaagtcaaa aaccagggga ggctcaaaag ttaattctcg gcgcaaaaca tattctttgg
      301 ttttttctaa gccttcaaat tccaccgcct ccactattcc ttctttaatg gtaaaactta
      361 gaactccttc ttcgctaaaa ttgatttctt ctactgctgt tattaaaaaa ccattatcag
      421 tataaagcgc ttttatgcgc tccaaatcat gttgaaatat tttccaattc ataatctgac
      481 ctggagatag aatcataact tttctaattt catcataact taaggcggtg ttaccagaaa
      541 tgttaatttc tttaaccggg ggaaactctt gcaccttaaa aattagctct ataccatcag
      601 ggtattcttt ggtttctacc catactttag agaaataccc cagatcataa atagatttaa
      661 cgtccgcttg cactttttcg gggttataag gttccttaag agaggtatat acagcagata
      721 aaatcaattg ttcgcttatg ttttcattgc attctactcg aatagctacc actggtggag
      781 aaacagtttg agcataaacc attcctccca ttcccaatac taacaaaaat accaaccccc
      841 aaaacaacct tttaattttt ttctgaacca atttacaacc ctcctccact atcaaaattt
      901 agtgttaaat tcaagcatcc agttaatttc tccttcagtg tctccctcca ttttcagaga
      961 ccattcttta tcaaagttta ttttgaaatc ccaggaagaa ttaccctcac cactcaaatc
     1021 ttgagtataa cctataaagt aattttcacc aatacttttc tccaatctta agccataaaa
     1081 gtcagaataa tctgaaccgg gaaattttat ctctactcca tcaaaaagtc cacccttgga
     1141 aaaaaaatct ttccctaata aaaattgccc caatttccag agaagaggtt gccaattaag
     1201 gttatccaga gagagatagg catctttttc tcctagaaaa agcaaagaaa gaatttcctc
     1261 ttcttcaagg agaggttctg attttaaaat taacttataa ttatctacag ggcccatagc
     1321 ctccagaaaa atatgataac ctcctatctc ctcttctcca tacaaattta ctcgaggatt
     1381 caacccataa agaggactaa aggtgatata tcctctaatg ggaatttttc tcaacaccaa
     1441 atcatagttt ccttccacca cttctagctt accctccccg ttgattctcc cgcttcctcc
     1501 ccaaattttg agtcttcctt ccatctccaa atctaaaaag cgagttttta ctctcaattt
     1561 attagcagta taaagggtaa gttccaagga aaggggcagt tcttctaatt tctcttctag
     1621 ttgagaaaga agagaagagt aattttcagt atcccctcct ttggtaaaat ctccccagcc
     1681 atcgaaaatt ttcgcttccc cctggagatt tattattcca tcacaggctc taattacaac
     1741 attactctcg actctagata gccccacatt ctcttctggt atttctaatt ttccacctcc
     1801 tgaaagatga atttcagact cttctcgaga aataactcct tctattatcc aattaagacc
     1861 agcgatatgc aagctcccac ttccgagctg aatttcccct tcctttatta ttcctcgtac
     1921 attgttaaaa tcagcatatt tctttccctc tcgccaaatc tcaccctccc cctccacttc
     1981 tccttgccag agaggttttt cttcatcacc agaaatagca atattggctt ctcctctcaa
     2041 ttgtagcact ccataaggaa tagaaa
//
LOCUS       DAOPSS010000178         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_44557_length_1992_cov_9.186371, whole genome shotgun sequence.
ACCESSION   DAOPSS010000178 DAOPSS010000000
VERSION     DAOPSS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44557_length_1992_cov_9.186371"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1437
                     /gene="tilS"
                     /locus_tag="PKN31_07945"
     CDS             82..1437
                     /gene="tilS"
                     /locus_tag="PKN31_07945"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOA99886.1"
                     /translation="MHSLVSKVRKFIVKYSLISPGDKVLVAFSGGPDSTALSQILLYL
                     REIIGFEMALFHLNHGLRGEEARRDENFCISWAKDKGVPIFVERQDVMSEKTAQRLSL
                     EEAARVVRYQMMREVAQVWGASKIALGHTMDDQGETVLLNVFRGTGLQGLQGMKFKND
                     IFIRPLLAVRREEILEFLEEEGIPFVEDSSNRDVSFLRNRLRNLIPVLEKEFNPQLIP
                     ALFRLSLNVQKEEASEPEVTVPIEKKGALSRTPLSYFKDVSSPELLKLVRSFIKEARG
                     NLWDISRGQIEEVANLLKKGKGETSLPGEFKVFIRDGYLWASPGSFFLADVPMWSFPL
                     QLPGENFFPDLGIAIRGNWKGEERKKTLQFVAWDFEKCCPPFQVRNFRNGDRIWQGGK
                     EIKVKELFSRQGIVREWRKLIPFICDREKILWIPGLALDERIRVRENSKQILVVSISW
                     L"
     gene            1449..>1992
                     /gene="hpt"
                     /locus_tag="PKN31_07950"
     CDS             1449..>1992
                     /gene="hpt"
                     /locus_tag="PKN31_07950"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOA99887.1"
                     /translation="MEPFIEQILISEKEIQAKVKELGEKISKDYQGEELRVVGILRGA
                     FIFMADLVRNIKLPLSVDFMSISSYEDDCETTGVVRILKDLDKPITNRHTLIVEDIVD
                     TGLTLKHLKEVLSTRSPASLKVCALLNKKDRRVVKDLAIDYCGFDIPNYFVVGYGLDF
                     AERYRNYPFIFVLKPEYYQKR"
BASE COUNT          567 a          314 c          499 g          612 t
ORIGIN      
        1 agagcatccg gctcataccc ggagtgccag ggttcgaatc cccgcaccgc caccaataac
       61 agattttgat cctaatgacg agtgcactct ttagtaagca aggttagaaa gtttattgtt
      121 aagtattccc ttatttctcc tggagacaaa gtattagtgg ctttctctgg aggtcctgat
      181 tctactgctc tctctcaaat tcttctttat cttcgggaga ttattggctt tgagatggct
      241 ttgtttcacc tcaatcatgg cctgcgaggg gaagaagcaa ggcgagatga gaatttttgc
      301 atctcctggg ccaaggacaa gggagtgcct atttttgtgg aaagacaaga tgtaatgagt
      361 gagaagaccg ctcagcgcct ctctttagag gaagctgccc gggtagttcg ttatcagatg
      421 atgagagaag tagctcaggt ttggggggca agtaaaattg ctttaggaca tactatggat
      481 gaccagggag aaaccgttct tcttaacgtc tttcgaggta cgggtctcca gggtttacaa
      541 ggtatgaaat ttaaaaatga tatttttatt cgtcctttac tggcggtgag gagagaggaa
      601 atattagaat ttcttgaaga ggaaggtatt ccttttgttg aagacagttc taatcgggat
      661 gtttcttttt tgaggaatcg actgcgaaat ttaattcctg ttttagaaaa ggaatttaat
      721 cctcagctta ttccggctct gtttcgttta tctcttaatg ttcaaaaaga agaagcttca
      781 gaacccgaag taaccgttcc catagagaaa aaaggagcgc tgagcagaac gcctctttcc
      841 tattttaaag atgtttcttc cccagaattg cttaagttag ttcgttcttt tattaaagaa
      901 gctcgaggta atctgtggga tataagtcgg ggacaaattg aagaagtggc taatttgctg
      961 aagaaaggaa aaggagagac atcgctgccg ggagaattca aagtttttat aagggatgga
     1021 tatctctggg cttctcctgg ttcatttttc ctggctgatg ttcctatgtg gtcttttcct
     1081 cttcaacttc ctggtgagaa cttttttccc gatttgggaa tagctattag aggaaattgg
     1141 aaaggagagg agagaaaaaa aacgcttcag tttgtggctt gggattttga gaaatgttgc
     1201 cctcctttcc aggttcggaa ttttcgaaat ggagatagaa tctggcaagg aggcaaagag
     1261 ataaaggtta aagagctttt ttcccgacag gggatagtga gagaatggag gaagttaatc
     1321 cccttcatct gtgataggga aaagatacta tggattcctg gtttagccct tgacgaaaga
     1381 ataagagtta gagaaaatag caaacagatt ttagtggtgt ctattagctg gttgtgagag
     1441 gtggcagagt ggaacctttt attgaacaga ttttgatttc ggaaaaggaa atacaggcta
     1501 aagtgaaaga gctgggagag aaaatcagta aagactacca gggtgaggaa ttaagagtgg
     1561 tagggatttt gcgcggtgct tttattttta tggctgattt agtacggaat attaagcttc
     1621 ccttaagtgt tgactttatg tcgatttcaa gctatgagga tgattgcgaa accactggag
     1681 tggtgaggat acttaaagat ctggataaac ctattactaa tcgtcatact ttaatagtgg
     1741 aagatattgt ggatactggt ctaaccctga agcatcttaa ggaggtacta tctactcgta
     1801 gcccggccag tctaaaagtg tgtgctttgt tgaataaaaa ggataggcgt gtggtgaaag
     1861 acttagcgat agattactgt ggttttgata tacctaatta ttttgtggtg ggttatggtc
     1921 ttgattttgc agaaaggtac cgtaattatc cttttatatt tgttttaaaa ccagaatatt
     1981 atcaaaaacg gc
//
LOCUS       DAOPSS010000179         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_44891_length_1982_cov_8.720810, whole genome shotgun sequence.
ACCESSION   DAOPSS010000179 DAOPSS010000000
VERSION     DAOPSS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44891_length_1982_cov_8.720810"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..737)
                     /locus_tag="PKN31_07955"
     CDS             complement(90..737)
                     /locus_tag="PKN31_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOA99888.1"
                     /translation="MFKLFIWDLDNTLIASSPLLWGAFSWVAEKYGNKKMTPSEIVSL
                     YGPPEDVVIERIVGKEKKDEALREFYRFYQEKHDELVGVFPLLLEIINYLRGHGVKQA
                     LFTSKGRKSAEITLQKLNIKDLFDLVVCGDEVARPKPYPDGVIKILETLLIKPEEALY
                     LGDSPLDAEAAKSAGVRFALTLWDSLPREEEEEKEVEWVFHSPEELWEWVQKVYN"
     gene            complement(730..1782)
                     /gene="hisC"
                     /locus_tag="PKN31_07960"
     CDS             complement(730..1782)
                     /gene="hisC"
                     /locus_tag="PKN31_07960"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOA99889.1"
                     /translation="MSYFREAINFMEEYIPGEQPEKEGYIKLNTNENPYPPSPLVEKK
                     IKEALGLGKISLYPHPLGGELRERAQEVYGLPLDWIVVGNGSDELLNLLFRLFLDKGD
                     RVAYPFPTYTLYRTLAFLQEAEREEIPFPPDFSLPESFLTSPAKLKVVCNPNSPTGTF
                     LPLSQIEKLLSLSSCPVIVDEAYVDFAPCSALPLLQKFPNLIIVRTFSKSFSLAGLRV
                     GLLFAHPDIVRGVLKIKDSYNVGILSQAGGAAALEDMEHMRKNVEKIKSTREWFSAEM
                     EKLGFLVYPSQANFIMARKEGRDLKFLYEELKKRKILVRYFPQWPDSLRITIGKEEEM
                     KTLLDTIQEIIGRVNV"
     gene            complement(1779..>1982)
                     /locus_tag="PKN31_07965"
     CDS             complement(1779..>1982)
                     /locus_tag="PKN31_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOA99890.1"
                     /translation="TFLNCPARRIVEVPVHYCCFEIPDVYVVGYGLDFQGDFRNLLEV
                     YTLYDPSGRSTQILKQMGKGGKK"
BASE COUNT          570 a          452 c          366 g          594 t
ORIGIN      
        1 taaatggctt tagtaatttt cgctggagag aaatcttcta attctccatt tctttttctc
       61 accttttccc aggacaaagc tatccctcct caattataaa ctttttgcac ccactcccag
      121 agctcttccg gagagtggaa tacccattct acctcttttt cttcttcctc ttctcgaggg
      181 agagagtccc agagagtaag agcaaagcgc actcctgcac ttttcgcggc ttctgcatcc
      241 aaaggagaat ctcccagata tagagcctcc tccggcttaa tcagcaaagt ctccagaatt
      301 tttatcaccc catcaggata aggtttggga cgagcaactt cgtctccaca caccaccagg
      361 tcaaacaaat cctttatatt taatttttgt aacgtgattt cagcgctttt tcttcccttg
      421 gaggtaaaaa gagcttgttt taccccatgc cctcgaaggt agtttattat ctccagcaaa
      481 agaggaaaaa ctccaaccag ctcatcgtgc ttttcctggt aaaaacggta aaattcccga
      541 agagcttcat ctttcttctc ttttcctact attctctcta ttaccacatc ttcaggtggg
      601 ccataaagac tgactatttc ggagggagtc attttcttgt ttccatactt ttctgccacc
      661 caggaaaaag ctccccacag aagaggagaa ctggctatta gagtgttatc taaatcccag
      721 ataaaaagtt taaacattta ccctccctat tatttcttgt atcgtatcaa ggagagtttt
      781 catttcttct tcttttccta tggtaatacg cagagaatcc ggccattggg gaaaataacg
      841 aaccagtatc tttcttttct ttaactcttc atagagaaac tttaaatctc ttccctcttt
      901 cctggccatg ataaaattag cctgggaagg ataaaccaga aaacccagtt tttccatctc
      961 tgcggagaac cattcccggg tagatttgat tttttctaca ttttttctca tgtgttccat
     1021 gtcttctaaa gctgctgctc ctcctgcttg agacaaaatt cccacattgt aggaatcttt
     1081 gatttttaaa acccccctca ctatgtcagg atgagcaaaa agaagcccca ctctaagacc
     1141 agccagggaa aaagatttgg aaaaggttct tactattatg agattgggaa acttttgaag
     1201 aagaggaaga gcactacagg gggcaaaatc aacatatgct tcatctacta ttacaggaca
     1261 agaagaaaga gaaagtaatt tttctatttg cgatagagga aggaaggttc cagtggggga
     1321 gttagggttg caaactactt ttaatttagc tggagaggtc aaaaaactct ccggaagaga
     1381 aaaatctgga ggaaaaggaa tctcctctct ctctgcttct tgaagaaaag ctaatgtccg
     1441 ataaagggta taagtgggga aaggataggc cacccgatct cctttatcta aaaaaagacg
     1501 aaaaaggagg tttagaagct cgtccgagcc atttcctact actatccagt caagaggaag
     1561 tccataaact tcctgagctc tttccctcag ttctcctcct aaaggatgag gatagagaga
     1621 aatcttgccc aggccgagag cttctttaat ttttttctct acgagaggtg aaggaggata
     1681 gggattttcg ttggtattga gttttatgta cccttctttt tccggttgct caccaggaat
     1741 atattcttcc ataaaattga ttgcctctcg aaaatagctc atttcttacc tccctttccc
     1801 atctgtttca aaatctgggt gcttctaccg ctggggtcat acaaagtata aacttcaagt
     1861 aagttacgga aatccccctg aaaatccaga ccgtagccta ccacatagac atcaggtatt
     1921 tcaaaacaac agtaatgtac aggaacttct acaatccttc ttgctggaca attcaggaaa
     1981 gt
//
LOCUS       DAOPSS010000180         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_48554_length_1870_cov_5.757576, whole genome shotgun sequence.
ACCESSION   DAOPSS010000180 DAOPSS010000000
VERSION     DAOPSS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48554_length_1870_cov_5.757576"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..578)
                     /locus_tag="PKN31_07970"
     CDS             complement(243..578)
                     /locus_tag="PKN31_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99891.1"
                     /translation="MDISQFLSLLLHELSNLEFVEKVDFHTEVFILKGRVFLQKERFL
                     QVYFNELTGTVAFALIEQGKRIWGVDYDKGRGWHLHSVDHPEAHENINPPTIEEIVAF
                     LSRAWGLLD"
     gene            complement(569..883)
                     /locus_tag="PKN31_07975"
     CDS             complement(569..883)
                     /locus_tag="PKN31_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99892.1"
                     /translation="MLTISLISPKLGELLIKTTKVSDIDHALEKVFKEYIDLKLKDLD
                     MKIKEFENKWKMDFNEFKTRLEKGTLPQDSYSFEVEQDFWEWEEAESLKAHYESLKEE
                     WT"
     gene            complement(1244..1504)
                     /locus_tag="PKN31_07980"
     CDS             complement(1244..1504)
                     /locus_tag="PKN31_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99893.1"
                     /translation="MVSSQREARKPQEDYNLRRDLQSLKSLRGKKKPSVPTSFSLFFL
                     ALAGEKFQDGLPRKGLELLIFQKEALFSILQLSSSEPMANII"
BASE COUNT          466 a          403 c          373 g          628 t
ORIGIN      
        1 aattttttat caggcatcca ctgaggaaga tgttttccct tcctcagtag tctttgtttc
       61 ctattatgtt ttttcagctt ttccttgttt ccggggggtt atgagggggt ctttttcccc
      121 cctcatagtt gtttgcaagg gggaaaactt ttttcttctc cagcgctgag gaaacatcac
      181 ctcggcgtgg ttgttgatgc tggtgtcctt gagctaatat ggttagaaga gaggtaaatt
      241 tttcaatcca gaagacccca tgctctagat aaaaaagcta ctatttcttc tatagttgga
      301 ggatttatat tttcgtgagc ttcaggatgg tcaacagagt gtaaatgcca tcctctcccc
      361 ttatcataat caacacccca aattctcttt ccttgctcaa tcagggcaaa agctactgtt
      421 ccagtgagtt cattaaaata gacctgtaaa aacctctcct tctggagaaa gactctacct
      481 ttgaggataa aaacttcggt atggaagtca actttttcca caaattcaag atttgatagt
      541 tcatgcaaaa ggagagagag aaattggctt atgtccattc ttcttttaat gattcatagt
      601 gagcctttaa actttcagcc tcttcccatt cccagaagtc ctgttctact tcaaaggaat
      661 aagaatcctg gggtagggtt cctttttcca atctggtttt aaattcatta aagtccatct
      721 tccatttatt ctcaaattct tttatcttca tatcgaggtc tttcaacttc aaatcaatat
      781 actctttaaa taccttctcc agggcatggt cgatgtcact aactttggtt gtttttatta
      841 acaattctcc aagttttgga gatataagag atatggttag cacccccttt cagcaagaga
      901 atgtctcttg cctcaacttt accacgtctg agtcaaaaac ttttcttttt accctcttgt
      961 ccctttgctt cccatctatc tatgttcttt ttatattata gcatgctcat ctacgataga
     1021 ttatggtgct ctgcaaaacc tgtcatttaa ctatcattgt gaaggaagct ggagattaac
     1081 cgctcctcgt attttcttct cccacccccg gagagagtgc ccgggttccc tgccaaacga
     1141 ttgtttggca gggtgggtaa gggaggggga aagaaagggg ttcttttttt ctattttcca
     1201 aaaatttttc cctcccccct attaacgaat tcagaaagag atgctatatt atatttgcca
     1261 ttggttctga ggagctaagc tggaggatgg aaaaaagagc ttctttttga aagatgagaa
     1321 gctccaagcc tttgcgcgga agtccatcct gaaatttctc gcctgcgagg gcgagaaaga
     1381 aaaggctaaa gcttgtcgga accgatggtt ttttctttcc ccttaaactc tttaaactct
     1441 gtaaatccct tctcaggtta tagtcttctt gtggctttct tgcttctctt tgcgagctca
     1501 ccattcctgg tggggcaatc tgttttcctt ttctgaggta atttaataaa gagatcgcca
     1561 cgcatggcaa aatacgccat gctcgcgatg acaagaaaga aagggagggt ttcttcttct
     1621 cttatttgga ttcctcgtag aaccattcag ggttgacgac cttctcctcc gtcattcccg
     1681 aaatttttta tcgggaatcc aaaggttttt ttgccctttt tctttctttt tggaactgta
     1741 ggggcgtcac ctcccattat ttggattcct gatagagaca ttcaggaatg acagacaacg
     1801 gggtgataga accattcagg aatgacgggc aaggtgatgg tggaggcgtt caggagtgac
     1861 gacctcctct
//
LOCUS       DAOPSS010000181         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_51906_length_1781_cov_9.038239, whole genome shotgun sequence.
ACCESSION   DAOPSS010000181 DAOPSS010000000
VERSION     DAOPSS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51906_length_1781_cov_9.038239"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="PKN31_07985"
     CDS             <1..154
                     /locus_tag="PKN31_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOA99894.1"
                     /translation="GLKKVGENQLIVISALDNLIKGAAGQAVQNLNLMFGKEEKEGLP
                     EDVLYP"
     gene            165..1379
                     /gene="argJ"
                     /locus_tag="PKN31_07990"
     CDS             165..1379
                     /gene="argJ"
                     /locus_tag="PKN31_07990"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOA99895.1"
                     /translation="MESWKILEGGLDNVSGFSSYGVHCGIKKGKNDLAAILSDTPAAA
                     AGVFTQNRVSAAPVLVTREHLLKERKLQLVVANSGVANACTGERGIEDARKVADLAAR
                     YFKVDHRLVAVASTGVIGEFLPLTKIEKGIEELSQLLPVKKSSREATKAIMTTDTFPK
                     ERALSFSWEGKTVHLGGIAKGSGMIKPNLATMLSFIVTDLGIDALVLDQVLKEAVNQS
                     FNRVTVDGDTSTNDMVLILANGKSGVEVKKSNDPVFSIFREALFWLTQELARDLARDG
                     EGATKFVEIIVRGARTKGEAQKGAFTVAESPLVKTALFGEDPNWGRIMAALGRSGIEM
                     IPDKVSLEVNGVTLVEGGMKAKGVEREEAQEAMKKKELKIIVDLGIGEGEFEVWTCDL
                     SLEYVRINSHYS"
BASE COUNT          600 a          266 c          487 g          428 t
ORIGIN      
        1 gggactcaaa aaggtgggag agaatcagct gatagtaatt tctgctctgg ataacttgat
       61 taaaggagca gccgggcagg cagtgcagaa tttaaatctg atgtttggga aagaggaaaa
      121 agaaggtttg ccggaggatg tattataccc ttgaggaggt agagatggag agctggaaaa
      181 ttttagaagg tggattggat aatgtttcag ggttttcttc ctatggagta cactgcggaa
      241 taaagaaagg gaaaaatgat ctggcagcta ttctctctga tacaccggct gcggcagcag
      301 gagttttcac tcaaaatcga gtttcggcag caccagttct ggttacccga gagcatttat
      361 taaaagagag aaagttacaa ctggtggtag caaacagtgg agtagctaat gcgtgcaccg
      421 gggaacgagg aatagaagat gccaggaaag tggctgacct tgctgctcgt tattttaagg
      481 tagatcatcg gctcgtagcg gtggcttcta cgggggtgat tggtgaattt ttgcctctca
      541 ctaaaataga aaaagggata gaagaattat ctcaattgtt accagtgaag aaatcgagca
      601 gggaagcaac caaagctata atgactactg atacttttcc caaagagcga gctctctctt
      661 tttcctggga aggaaaaact gtccacctgg gaggaatagc taaagggtca gggatgatta
      721 agccaaattt ggctactatg ttgtctttta tagttaccga tttaggcata gacgctctgg
      781 ttttagatca agtattaaaa gaggcggtga atcaaagctt taatcgggtg acggtggacg
      841 gagatacgag tactaatgac atggtattga ttctggctaa tggtaaaagt ggagtagaag
      901 ttaagaagag taatgatcca gttttttcca tttttcgaga ggctcttttt tggttaactc
      961 aagagttagc tcgggattta gcccgagacg gagagggcgc cactaagttt gtagagataa
     1021 tagtgcgagg agcgagaaca aaaggagaag cacaaaaggg tgcttttacc gttgctgaat
     1081 ctcctctggt aaaaactgct ctttttggag aagaccctaa ctggggaagg attatggctg
     1141 ctctggggcg aagcggaata gaaatgattc ccgataaagt ttctttagag gttaacggag
     1201 ttactttggt agagggagga atgaaggcta agggagtgga gagagaagaa gctcaggagg
     1261 cgatgaagaa aaaggagctt aaaattattg tagacctggg aataggagag ggagagtttg
     1321 aagtgtggac ttgtgattta tctctggaat atgtacggat taatagccat tatagctgac
     1381 agcaacctta ttgcccccca aaaaattaaa tacagaataa aaagataaaa atcaccgagg
     1441 gagaagggag agaaaacaga cggtctgcga cgcaagataa aggcaaaata gataaaaaag
     1501 attcagaaaa ataacgttgg gaaaatatcc cagcgtgcta cgacgtaaga ctaaaagtat
     1561 gaaaaacaaa aaattaaaat taaaaggaca agaacaactg aggaagaaaa tgccttcctc
     1621 agtgaagggg aaaagaactc ccggaatatg agagggaaac actccccttc gatcctcagc
     1681 ggcgagggga gcaagataaa gtgcaaaaga gaaaaatgac atgatgtttg tgattaatta
     1741 ttatttcctg taaatcagga ggaggaaact atgaagatag t
//
LOCUS       DAOPSS010000182         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_52096_length_1776_cov_7.175479, whole genome shotgun sequence.
ACCESSION   DAOPSS010000182 DAOPSS010000000
VERSION     DAOPSS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52096_length_1776_cov_7.175479"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PKN31_07995"
     CDS             complement(<1..1110)
                     /locus_tag="PKN31_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA99896.1"
                     /translation="MTSEERILKTLSHQEPDHVPFDLGGTIVTGITKGIYSRLLEYRG
                     LTREVRVLDYVQQLAQIDEDVARWLEIDTKCGFPGISRKNPLIKEEGNYFYFEDEWGI
                     RWGMPKSGGLYFDVISNPLAKAENIDDVEKYPWPDPGAEILKDLEEEIGRQNQEKILI
                     VESFGSGIFETALRLRGYEQFLIDLTLAPGLAEAILEQILEVKLRYIDLLNQNFKGKI
                     HILREGDDLATQQSLLISKEMYQRFIKPKHRQLFEYAKKSLGPSFYIFFHSCGAVYDL
                     IPDFIEAGIDILNPVQLSASKMNPEKLKRDFGKLITFWGGGVDTQGTLLRGSKDDIRE
                     EVKRNIETFAPGGGYVFAAVHNIQDDIPVENLVTMW"
     gene            complement(1123..>1776)
                     /locus_tag="PKN31_08000"
     CDS             complement(1123..>1776)
                     /locus_tag="PKN31_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOA99897.1"
                     /translation="EVDGIILGSSRIKEEQIREIAEGDIPCVIINRIIENVPSIFADY
                     ENGAYQATKYLVNLGHERIALINGPNSAQPSLWREKGFLRALKESGIEVDESLLSFNS
                     PVVEGGYVATLKLLSIDNPPTAIFAYNDLVALGAMKAIREKNLLVSKDISVVGYDDIF
                     LSAYLDPPLTTVSQPKYMMGKLAADLLFRLINGEKVKNSQISLKPELIIRSSCGVRV"
BASE COUNT          533 a          410 c          277 g          556 t
ORIGIN      
        1 ccacatagtc accaagtttt ctaccggtat gtcatcttga atattatgta ctgctgcaaa
       61 tacgtagcca cctcctggag cgaaagtttc gatattcctt tttacctctt cacgaatatc
      121 atctttactc ccccgcaata aagtcccctg ggtgtctaca ccacctcccc aaaaagtgat
      181 cagttttcca aaatcccttt ttaacttctc aggattcatt tttgaagcac taagctgaac
      241 agggtttaat atatcaatcc ctgcttcaat aaaatccggt attaaatcat aaactgctcc
      301 acaagagtga aaaaagatat aaaaagaagg acccaacgat ttttttgcat actcaaaaag
      361 ttgtcggtgt ttaggtttta taaaccgctg atacatttct ttagaaataa ggaggctttg
      421 ttgagtagcc aggtcatctc cctctctcaa aatgtgaatt tttcctttaa aattctgatt
      481 tagaagatcg atatagcgca atttaacttc taaaatctgt tccaaaatag cttcagccag
      541 accaggagca agagttaaat ctattagaaa ctgttcatag cctcgcaacc gcaaagcagt
      601 ttcgaaaatg cccgaaccaa agctctctac tattaatatc ttttcttgat tctggcgacc
      661 tatctcttct tccaggtcct ttaaaatttc ggctcctgga tcaggccagg gatatttctc
      721 aacatcgtca atattttctg cctttgcaag tggattagaa attacatcga aatataaacc
      781 tccacttttt ggcatgcccc atcgaatgcc ccattcatct tcaaaataaa aataattccc
      841 ttcttcttta atcaaaggat ttttgcgaga tataccaggg aaaccacact ttgtatctat
      901 ttccaaccag cgagccacat cttcatctat ctgagctaat tgctggacat agtcaagaac
      961 tcgaacctcc cgggtcaaac ctcgatactc cagcagtctg gaataaatac ctttcgtaat
     1021 tccagttact atagtccctc ccagatcaaa aggaacgtgg tctggttctt ggtgagataa
     1081 agtttttaaa atcctctcct cggaagtcat tttagtattc tcctaaactc ttactccaca
     1141 agaagacctg attatcaatt ctggtttcaa acttatctga ctatttttga ctttttctcc
     1201 atttattaat cggaaaagta aatcggcagc taatttaccc atcatatatt tgggctggct
     1261 gacagtagtt agcggcggat cgaggtatgc ggaaaggaaa atgtcatcat aaccaaccac
     1321 ggaaatgtcc ttagaaacca acaaattttt ctcccttatt gccttcatag ctcccagagc
     1381 tacaagatcg ttataagcaa aaatagcagt aggagggtta tcaatactta ataatttaag
     1441 agtggctacg tatcctcctt ctacaacagg agaattaaag ctcaaaaggc tctcatcaac
     1501 ttctattcct gattctttta aagctctcag gaatcccttt tctcgccaca aactgggttg
     1561 agcactattt ggaccattga ttagagcaat tctttcatgt cccaaattca caagatattt
     1621 tgttgcttga taagcaccat tttcgtagtc agcaaaaata ctgggaacat tctcaattat
     1681 cctatttata attacacagg gaatatcgcc ttctgctatt tctctaatct gctcttcctt
     1741 aatccgggag gaccctaaga taatgccatc tacctc
//
LOCUS       DAOPSS010000183         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_54414_length_1720_cov_5.310511, whole genome shotgun sequence.
ACCESSION   DAOPSS010000183 DAOPSS010000000
VERSION     DAOPSS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54414_length_1720_cov_5.310511"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..>1720)
                     /locus_tag="PKN31_08005"
     CDS             complement(365..>1720)
                     /locus_tag="PKN31_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOA99898.1"
                     /translation="IEVIENTMIQGILLDEENRVKGAWGIGILDNDYFFIDAPAIIIA
                     TGGAGQVFLENLFPPLATGDGWTAALRAGAEFVNMEFIQIGLSSKETKIACSGSFMRS
                     LPEIVNSEGEDILLKYYKDKYSQEELINILFSKGWSWPLSYEEESHIIDVAVAKEIQE
                     GRQVFLSYTQNPDFLEKDSLPERVLEWYRSSLGEDLVRGDLFTSPLLRLKKMNREVYE
                     LLRSKGINLDEKGKFEIVPAVQHFQGGIKINEKAETNIEGLYACGEAAGGQHGANRPG
                     GNSLLDTQVFGKISGQEAAIYSSSVKRSKITGEEVETIKKQFPRFSREVLGGNWIRQS
                     IREIMTRNAGVIRVPHNLREAYKEISSITEREILNVSESLKDYLETLNIHLLSQLLLL
                     TMYERKESRGPHLLFKNEDSMELYPRDDGAWAYHYIVAKLKYGAITLEKRPVNRGNSK
                     R"
BASE COUNT          477 a          422 c          244 g          577 t
ORIGIN      
        1 gagttttcaa ccccaggata gaacaaccag ctaaaacatt ggcctccgcc cctcccactt
       61 ctacctgcca ggaatcgctt ttcaaaaaag ttttaagtct ttccggggtc agacgaatca
      121 aaggttctcc atagcttata atatcagttt tcataatatc atccttccaa atcttacctt
      181 aagttctatc atttttccca aaaccccctc agattaagct tttatgtatt ttcatcctcc
      241 aactatgttg aaaaggaagt actttgctac ttccagatac cacgctttag cccttatagc
      301 atgcagcgtc ccaattctcc tcctgttttc tgatcgggga agagcaaact actaaaagat
      361 agttttatct tttagagttt cccctattaa ccggtctttt ttctaaagtt atagctccat
      421 atttcagttt agcaactata tagtgataag cccaagctcc atcatctcga ggataaagct
      481 ccatactatc ctcattttta aatagtaaat ggggacctct actttctttc cgctcataca
      541 tagtgaggag aagcaactgg gatagcagat ggatatttaa agtctctaaa taatctttca
      601 agctttcgct aacattgaga atttctcttt ctgttatact ggaaatttcc ttataagctt
      661 ccctcaaatt atggggaacc cttataacac cagcatttct ggtcataatt tctctaatac
      721 tttgccttat ccagttccca cccaaaactt cccgggaaaa tctcggaaac tgctttttta
      781 ttgtttctac ctcctctcca gtgattttgc ttctctttac tgaagaagag taaattgctg
      841 cttcctgacc tgatattttc ccaaaaacct gtgtgtcaag caaagaattt cctcctggtc
      901 tgttagctcc gtgttgcccg ccggcagctt ctccacaggc gtacagtccc tctatgtttg
      961 tttcagcttt ttcatttatc tttattcccc cttgaaaatg ctgcactgca gggactattt
     1021 caaatttccc tttttcatct aaatttattc ctttacttct aagtagttca taaacttctc
     1081 tgttcatttt tttaagtctc aataagggtg aggtaaataa atcgcctctt accaaatcct
     1141 cccctaatga gcttctatac cactctaata ctctttccgg cagggaatct ttttctaaaa
     1201 aatcgggatt ctgagtatag cttaaaaaaa cctgtctccc ctcttgaatt tctttagcca
     1261 cagccacatc aataatgtga gattcttcct cgtaagagag tggccagctc catcctttgg
     1321 aaaaaagaat atttattaat tcttcctgag aatatttatc tttataatat ttaagtaaaa
     1381 tatcttctcc ttcactgtta actatttcag gtaaagatct cataaaactc cccgaacagg
     1441 ctattttagt ttccttagaa gacaagccta tctggataaa ctccatattt acaaactctg
     1501 caccagccct caacgctgct gtccatccat ctcccgtagc cagaggagga aagagattct
     1561 ccagaaacac ctgtcctgca ccaccggtag ctataattat ggccggagca tcaataaaaa
     1621 aataatcgtt atccagaatc cctattcccc aggcaccttt aaccctgttt tcttcgtcca
     1681 ataaaatgcc ttgaatcata gtattttcta taacttctat
//
LOCUS       DAOPSS010000184         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_56261_length_1678_cov_9.084412, whole genome shotgun sequence.
ACCESSION   DAOPSS010000184 DAOPSS010000000
VERSION     DAOPSS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56261_length_1678_cov_9.084412"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKN31_08010"
     CDS             complement(<1..313)
                     /locus_tag="PKN31_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOA99899.1"
                     /translation="MQKRAEKLSPATLILSGFILIILSGTFVLLLPFMVNPGNKLRFI
                     DALFTATSATCVTGLIVVDTATFFSFWGQLVILVLIQIGGLGYMTLNTILALALRRRI
                     EY"
     gene            complement(373..1503)
                     /locus_tag="PKN31_08015"
     CDS             complement(373..1503)
                     /locus_tag="PKN31_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA99900.1"
                     /translation="MLGRERALMSLNHQVPDRIPLDLGSTNCTTMTKVAYDNLKKYLG
                     IERETRFMMENFQIVFIDEEVLQILEIDTRGVHPRPVFFKNMVDDRTYFNEFGIKYHM
                     PEEGLYYDMVEHPLAGKSIEELKDYEWPDPARTMDLSGVRERAKNLKESNQYLLVGDM
                     IDTGIFEPCWYLRGFENFLVDLLTDQDFVTTLLEGMYNYQLKRYSLFLQEAGEFLDVV
                     FVGDDLATAESVIMSPKTYRDIIKPYHKEYFKNLKKLAPHARLLYHSCGSIFSFIPDL
                     IEVGVDILNPVQVSAQNMDTKVLKEKFGGELSFWGAIDTTRVLPRGTKKEVKEEVRKR
                     IDDLGPEGYILTSVHDIQPDVPPENVITMFEEARNYGVFLSH"
BASE COUNT          515 a          386 c          270 g          507 t
ORIGIN      
        1 gatattcaat cctccgccgg agagctaagg ctaaaatagt atttaaggtc atatacccca
       61 gccctccaat ctgaatgagc acgagaatca ccagttgtcc ccagaaggaa aaaaaggtag
      121 ccgtatctac cactattaaa ccagtaacac aagtagcaga agtagcagta aataaagcat
      181 ctatgaaacg caatttattt cccgggttca ccataaacgg caaaagcaga acaaatgttc
      241 cactcaaaat gataagaata aaacctgaaa gtattaaagt agcaggagaa agtttttccg
      301 ctcttttttg cactttttcc cggagagaga atgattctct gccctcggat aagaccaccc
      361 gtatcaacct tctcagtgag agagaaaaac cccgtaattt ctggcttctt caaacatagt
      421 tattacattt tccggaggaa catcaggctg aatatcatgc actgaagtta aaatataccc
      481 ttccggaccc agatcatcta ttcttttcct tacctcttct tttacctctt ttttagttcc
      541 tcgaggaaga acccgggtgg tgtctattgc tccccagaaa gagagttccc ccccgaactt
      601 ttcctttaat actttagtat ccatattctg agccgaaacc tgaaccggat ttaagatatc
      661 tactcccacc tctattaaat cgggaataaa agaaaaaata cttccacagg agtgatagag
      721 aagccgagca tgaggagcca gcttttttaa atttttgaaa tactccttgt gataaggttt
      781 aattatatct cgataggttt tgggactcat aattacactt tcagcagtag ctaagtcatc
      841 tcctacaaaa accacgtcta gaaactctcc tgcttcctgg agaaaaagag aatacctttt
      901 tagttgataa ttgtacatcc cctctaacag ggtagtaacg aaatcctggt cggtcaaaag
      961 gtccaccaaa aaattttcaa agccccggag ataccagcaa ggttcaaaaa taccggtatc
     1021 aatcatatct cctaccagaa gatactggtt gctctcttta agattctttg ctctttccct
     1081 cactcccgag agatccatag ttctggccgg gtctggccac tcgtaatctt ttagttcttc
     1141 tatgcttttt ccggctagag ggtgttctac catatcatag taaagacctt cctcgggcat
     1201 atgatacttg ataccaaatt cattaaaata ggttctatca tccaccatat ttttgaaaaa
     1261 aactggccgg ggatgaacac cccgagtatc tatttccaga atctggagca cttcttcatc
     1321 aataaaaaca atttgaaaat tctccatcat aaaccgagtt tctctttcta ttcctaaata
     1381 tttctttaaa ttatcataag ccactttggt catagtagta cagttagtac tccctaaatc
     1441 cagaggaatt ctatccggaa cctgatgatt cagtgacatt agagctcttt ctcttcccag
     1501 cattttatcc tccctttttg ttttagagac ttataataaa cttagtgaat tttataactt
     1561 tccattgaaa attatccttt atattttacc agaataagca aactttattc gaccctctgc
     1621 ctcaacttga aactcaccag gtgaggtaag gaattcttct tctatatgct cccacttt
//
LOCUS       DAOPSS010000185         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_57024_length_1662_cov_7.158681, whole genome shotgun sequence.
ACCESSION   DAOPSS010000185 DAOPSS010000000
VERSION     DAOPSS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57024_length_1662_cov_7.158681"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            419..493
                     /locus_tag="PKN31_08020"
     tRNA            419..493
                     /locus_tag="PKN31_08020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:451..453,aa:Val,seq:cac)
     gene            complement(514..1179)
                     /gene="rpe"
                     /locus_tag="PKN31_08025"
     CDS             complement(514..1179)
                     /gene="rpe"
                     /locus_tag="PKN31_08025"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOA99901.1"
                     /translation="MIKVSASLDCADYLNLGEEVKKLEEAKIDMLHIDIMDGVFVPNF
                     ALGTNLLRKLRPATSLLFDVHLMVQNPESFIPLFADLGADMITFHAEVSSRLHQLVDR
                     IKKEGKKVGLALNPSTPPELLSYLLPYLDLVLLMTVDPGFVGQKFIPEVIPKVEKVRE
                     MVEKKGLNLDIAVDGGIGEKTVPPLKRAGANVFVAGTTSIFSGREDVEKAALEFKEFC
                     EKC"
     gene            complement(1196..>1662)
                     /locus_tag="PKN31_08030"
     CDS             complement(1196..>1662)
                     /locus_tag="PKN31_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOA99902.1"
                     /translation="LGGARMLTGYDVDEQKFPLARALGFDLTLSPAGEKEEVDLAIEA
                     SGSNRALLDALRIVRKKGKVLLVGNQGQDVNLNPQEIGIILRRELTVVGTWNSDFSSL
                     NSDWKRLLELEERGRFSLSPLISHRVTLEEAPSFIMEMQQKHFPYTKVIIEP"
BASE COUNT          530 a          354 c          294 g          484 t
ORIGIN      
        1 actttatcat atcatctcct cttatgtacg aagaatatga gaggttagtt cctcaaaccc
       61 ctcaaatagc gtgaaaaaac tgctaattta gaagataaag caagtcttct tgtgaaacta
      121 ttgtgaattg gaaagaagat tttctctccc agcgattaaa cagaatgaaa caatcacttt
      181 aagagtactc tttcatcttg acattagggc ctactttaaa accggcttta agaatgaaat
      241 acggacattg atgattacat tgatcaggaa agaaattatt gaacaaggta gtttgaaggt
      301 gcagaagaaa cagctttgaa aacatgattt attatagaat aaccacaacc gcttttgcca
      361 aggtattgaa gaaaatgata acactatggt agaatagtgt gtgtagaaaa gtttttgggc
      421 gcggttaact cagcggtaga gtgccatcct cacacggtgg aagtcgctgg ttcgaatcca
      481 gcaccgcgca ccattttatt taagaaaaga atcttagcat ttctcacaaa attccttaaa
      541 ctctaaagct gccttctcta cgtcttcccg accagagaaa atactggttg ttccagccac
      601 aaaaacatta gccccggctc tttttagagg aggaacagtt ttttctccaa tccccccatc
      661 tacggcgatg tccaaattca agcctttttt ctctaccatt tctcttacct tctcaacctt
      721 gggtatcact tcaggaataa atttctgtcc tacaaatccc gggtctacag tcattagaag
      781 taccaggtct aaataaggta aaagatagga aagaagctca ggaggagtag agggattaag
      841 agccagcccc acttttttac cttctttttt aatcctatca actaactgat gtagacgact
      901 gctcacctca gcatgaaaag taatcatatc tgctcctaaa tcggcaaata gaggtataaa
      961 agattccgga ttctgtacca taaggtgaac atcaaaaaga agagaggtgg ctgggcgaag
     1021 ttttcttaaa agattagtac ctagagcaaa gttaggaacg aaaactccat ccataatatc
     1081 aatgtgaagc atgtctattt ttgcttcttc taactttttt acttcttccc ccagattaag
     1141 ataatcagca caatctaaag aagcagaaac ttttatcacg atttgactga cctccttatg
     1201 gctctataat aactttagta taaggaaaat gtttttgctg catttccatt ataaaagagg
     1261 gagcttcctc cagagttact cggtgagaaa tcaaaggaga taaagagaat cttcctctct
     1321 cttctaattc caataatctt ttccaatctg aattcagaga cgaaaaatcg gaattccagg
     1381 taccaacaac ggtaagctct cggcgaagaa ttataccgat ttcttgagga tttaagttca
     1441 cgtcttgtcc ttgattcccc accaggagaa ctttgccttt ctttctcact attcttaaag
     1501 catctaaaag agcccggttg cttcctgaag cctcaatagc taagtccacc tcttcttttt
     1561 cccctgccgg agataaagtg agatcaaaac ctaaagcacg agctaaagga aatttttgct
     1621 catccacatc atatcctgtg agcattcgag ctcctcctaa ct
//
LOCUS       DAOPSS010000186         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Atribacteria bacterium isolate AMR_MDS_1464
            NODE_64688_length_1521_cov_7.051842, whole genome shotgun sequence.
ACCESSION   DAOPSS010000186 DAOPSS010000000
VERSION     DAOPSS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854669
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Atribacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Atribacteria bacterium
            Bacteria; Atribacterota; Atribacteria.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:12:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,608
            CDSs (total)                      :: 1,572
            Genes (coding)                    :: 1,566
            CDSs (with protein)               :: 1,566
            Genes (RNA)                       :: 36
            tRNAs                             :: 34
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 2 of 6
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Atribacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64688_length_1521_cov_7.051842"
                     /isolate="AMR_MDS_1464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053509"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKN31_08035"
     CDS             complement(<1..286)
                     /locus_tag="PKN31_08035"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873291.1"
                     /note="catalyzes the reversible phosphorylytic cleavage of
                     uridine and deoxyuridine to uracil and ribose- or
                     deoxyribose-1-phosphate; involved in the pyrimidine
                     salvage pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOA99903.1"
                     /translation="MNYYAYHLALREEEVKGIKLALLPGAPERCCKIASAFGDFEELA
                     SHREFKTVKAESEEGKILVVSTGIGGPSLSIAVEELAQLGVRLFLRVGTCG"
     gene            342..>1521
                     /locus_tag="PKN31_08040"
     CDS             342..>1521
                     /locus_tag="PKN31_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA99904.1"
                     /translation="MPKKTCYLYWEKEKIPVPQGKTLQEVAKEIFPSWERIMAAQLEG
                     EIVDLNTRVERDSKVKFFDFSHPEGRRTYLRSLLFVLSYAVEKTFPGRRLKVLYSLGE
                     GLICEIEGVNISPEQLEQIEEEMRNTIKEDLPFIREVIDWEEATAIYQKRGRKDLVKL
                     FRYWRTPAVTVYRLQDFYDHYYGPLVPSTGYLKNFGFVLVPPQFIIQFPTGEDLQRLP
                     TYTFRPKLFATFQEAVRWAEILAIENVGELNEAIARGEGREIISIAEALHEKKIAQIA
                     DLITQKKGTVHLVLIAGPSSSGKTTFARRLYIQLRVNGWHPITISLDDYFRSRGEINE
                     EDYEKPEALDLILFEEQIKALIRGEEVEIPRYNFITGTREPRGTKTQLSPDGIIIVEG
                     L"
BASE COUNT          476 a          362 c          307 g          376 t
ORIGIN      
        1 ctccacaggt tcccactcgc aaaaaaagcc ttactcccaa ttgagccagc tcttctaccg
       61 ctatactcaa agaaggcccc ccaatgccag tagaaactac caaaatttta ccttcctcgc
      121 tttctgcctt taccgtttta aattcccgat gagaagctaa ctcttcaaaa tcaccaaaag
      181 cagaagcaat cttacagcat cgctccggag cccccgggag gagagccagt tttatccctt
      241 ttacttcttc ttcccttaaa gccaaatgat aagcataata attcattact cttcacctct
      301 tctctatttt ctgctaaaat agggaaaagg ggaagaggta aatgccgaaa aaaacttgtt
      361 atctttactg ggaaaaagaa aaaatacctg tcccacaagg aaaaaccctc caagaggtag
      421 cgaaagaaat atttccctcc tgggaaagaa ttatggccgc tcaattagaa ggagagatag
      481 tagacctcaa caccagagta gaaagagaca gcaaagtaaa gttttttgac ttctctcatc
      541 ccgaaggcag aagaacttat ctgcggagtc tcctttttgt cctgagctat gcggtagaaa
      601 aaactttccc cggtcgccgg ctaaaggttc tttattccct gggagagggg ctaatttgtg
      661 aaatagaagg ggtaaacatc tctcctgagc aattagagca aatcgaggag gagatgagaa
      721 acaccataaa agaagacctc ccctttatcc gagaagtaat agactgggag gaagctactg
      781 ctatttatca aaagcgagga aggaaagacc tggtaaagct tttccgctac tggagaactc
      841 cagcagtaac cgtttatcgg ctgcaagact tttatgacca ttattatgga cctcttgttc
      901 cttctaccgg gtatctaaaa aactttggct ttgtcctggt tcctcctcaa ttcataattc
      961 agtttcccac cggagaagac ctgcagcgtc ttcccactta tacttttcgt cccaaacttt
     1021 tcgccacttt tcaagaagca gtccgctggg cagagatttt agccatcgaa aacgtaggag
     1081 agctaaacga agccattgcc cggggagaag gaagagagat catatccatt gctgaggcgc
     1141 ttcatgagaa aaaaatagcc cagattgccg acttaatcac ccagaaaaaa ggcacagtac
     1201 atttagtatt aatcgccgga ccttcttctt cggggaaaac cacttttgcc cgtcgtctct
     1261 atatccagct ccgggttaac gggtggcatc cgataaccat atccttagac gattacttcc
     1321 gttcccgagg agaaataaat gaggaagatt acgagaagcc agaagcttta gacttaatat
     1381 tattcgaaga acaaataaaa gcactaatcc ggggagaaga agtggaaatc cctcgttata
     1441 actttatcac cggcactcga gaaccccgag ggacaaaaac tcagctctct cccgatggta
     1501 tcataatagt agaaggatta c
//
